LOCUS       DATRNX010000001        65454 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRNX010000001 DATRNX010000000
VERSION     DATRNX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 65454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65454
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            241..411
                     /locus_tag="VBC81_000001"
     CDS             241..411
                     /locus_tag="VBC81_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911074.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            complement(452..652)
                     /locus_tag="VBC81_000002"
     CDS             complement(452..652)
                     /locus_tag="VBC81_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6911075.1"
                     /translation="MSQEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(807..1766)
                     /locus_tag="VBC81_000003"
     CDS             complement(807..1766)
                     /locus_tag="VBC81_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO6911076.1"
                     /translation="MAENFQHKSYREQVELLESRGIVFSGRKAKSKAEYSLSVISYYK
                     IKEFAKPFAKIQKDGQKKKIDYQGTKFEIVISRYYQDKNLRLNLLHAIEDIEVAIKTK
                     IAYVLGKNGLGSYGYLDFSKWCNKEEYCKHYLSYSENNFKKQLKRELRKASSSELDEK
                     LKLDRQKYPPIWLAVNMLTFGQMVNLLELMSTTNLTQISSTFSCSNPELISWLKCINL
                     ARNICAHNSNIIDFKFITVSKLKEEWKDFLFEYKEGVFSNRIALPFLIVLEMMGKINP
                     KYHFRDIIDSLHKLIKDEDTAKYYGFASMNTIKEIKKKKNFRG"
     gene            complement(2105..2824)
                     /locus_tag="VBC81_000004"
     CDS             complement(2105..2824)
                     /locus_tag="VBC81_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6911077.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
     gene            complement(2826..3164)
                     /locus_tag="VBC81_000005"
     CDS             complement(2826..3164)
                     /locus_tag="VBC81_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6911078.1"
                     /translation="MNEFTTQIITVSVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISAEVGGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVK
                     RMNDEWKKGQ"
     gene            complement(3166..3462)
                     /locus_tag="VBC81_000006"
     CDS             complement(3166..3462)
                     /locus_tag="VBC81_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911079.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNNNKEK"
     gene            complement(3472..3633)
                     /locus_tag="VBC81_000007"
     CDS             complement(3472..3633)
                     /locus_tag="VBC81_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911080.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            complement(3696..4112)
                     /locus_tag="VBC81_000008"
     CDS             complement(3696..4112)
                     /locus_tag="VBC81_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6911081.1"
                     /translation="MKQWNIVGKPFPIYQDGVVVKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIRLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            complement(4123..4275)
                     /locus_tag="VBC81_000009"
     CDS             complement(4123..4275)
                     /locus_tag="VBC81_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911082.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            complement(4284..8111)
                     /locus_tag="VBC81_000010"
     CDS             complement(4284..8111)
                     /locus_tag="VBC81_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6911083.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFNGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWTDQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQEILDNAVFKANQALASADGKNTNYYGTEMPVDDPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKNLSDSAKKDATEALTNLISESKKLTDKMSALSKTEVEHYSTTNLQLTQLDG
                     TVKGLQTSYEALLKKDGDITQTLANYKQTIDQNSTSITANKKTVDGTLSSLQTQVTQT
                     ANEITTRLSQTNIEALITSKSAVIADNKVKETADSFSREITRVNNTIDNLKIGTVNLL
                     SGTKNWTKNWFNRNNWQSDDDNLTIGNFSLTVLKRKAMWSGISQLYAVKSGENYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVTSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEDLTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGWFMVGGF
                     MLSKGTADLPWSESKQDIKDNVTAINTIVAQTANSWAIKNLTSNGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLTDLNTGQIEFYTDNPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            complement(8124..8966)
                     /locus_tag="VBC81_000011"
     CDS             complement(8124..8966)
                     /locus_tag="VBC81_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6911084.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSHTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            complement(8979..13205)
                     /locus_tag="VBC81_000012"
     CDS             complement(8979..13205)
                     /locus_tag="VBC81_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO6911085.1"
                     /translation="MAGTFDGAIFADIGANTKDYEAAMARIVSTTQNAFRKAQDAAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNSFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIAQKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGAMVASGLSTASGIIGAFAATVSGLPAIFATAAARGVAIL
                     GAMTQAMGAVVSVAMAAIGPAAILGLVVAGLGLVNNQFGTQIDKLLNIAITRGSQIIT
                     NLVNGITSQIPQLIASGAELISKFANAITVLLPVIIQAGVQLISSLVQGVGQNATSLI
                     SSAIQIIGTFISSIASALPQLLAVGMELIVNLVQGIVQNIPLIIQYAQQIINNFGASL
                     QANMPSIVNNGIAILTNLVQGIIQMLPAVVQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGLQLVVQLAVAIIKGLPQ
                     IISAGIQLIIGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMSGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INVIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            complement(13234..13464)
                     /locus_tag="VBC81_000013"
     CDS             complement(13234..13464)
                     /locus_tag="VBC81_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911086.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNISNDFAERVAKLNLHFQNL"
     gene            complement(13539..13994)
                     /locus_tag="VBC81_000014"
     CDS             complement(13539..13994)
                     /locus_tag="VBC81_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911087.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            complement(14045..14677)
                     /locus_tag="VBC81_000015"
     CDS             complement(14045..14677)
                     /locus_tag="VBC81_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6911088.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     TLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLKDREVYPAKFGYGKIDEIEYSEGVEDLVEASYALNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSSPGAVPEKLSEGH"
     gene            complement(14689..15048)
                     /locus_tag="VBC81_000016"
     CDS             complement(14689..15048)
                     /locus_tag="VBC81_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911089.1"
                     /translation="MIYSPSTLYLNEVKNRLGTLNIPIYFKLPKSDVLEPFIVIGSNV
                     SDTSKTAQTGAVVGDFGLNIDAFLPATSRTDAEEVKSRMIMSLGRNKRINAQLLMDNS
                     VGREVYHIAINITEIVI"
     gene            complement(15052..15396)
                     /locus_tag="VBC81_000017"
     CDS             complement(15052..15396)
                     /locus_tag="VBC81_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6911090.1"
                     /translation="MSLTYRMRGLDRFLRSVERKQKSARIAVDKKLSKSAARIERQAK
                     ILAPVDTTWLRSQIYNEQQKLLHYRVVSPVLYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            complement(15393..15671)
                     /locus_tag="VBC81_000018"
     CDS             complement(15393..15671)
                     /locus_tag="VBC81_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911091.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            complement(15682..16038)
                     /locus_tag="VBC81_000019"
     CDS             complement(15682..16038)
                     /locus_tag="VBC81_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6911092.1"
                     /translation="MDKELLLVEVKIFKGIAKNDASQDELIKLAIDESIERILAKLNE
                     YSDEELLEIPRRLTFIARDVAIKRYNRLNSEGAKADSEEGRSFSWEDYLTEYDSILRN
                     VAYGKRCRGKGVARFI"
     gene            complement(16044..16940)
                     /locus_tag="VBC81_000020"
     CDS             complement(16044..16940)
                     /locus_tag="VBC81_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6911093.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRREELT
                     SDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGAGLQKALSLSWAKLATFNEFE
                     GSQLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKVYST
                     AVDNLVFASLNVRGGDLGGLFADYTDETGLIAAARQRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEADKEVPGAVPGG"
     gene            complement(16959..17528)
                     /locus_tag="VBC81_000021"
     CDS             complement(16959..17528)
                     /locus_tag="VBC81_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6911094.1"
                     /translation="MAEENVATETTEQVDTQKEAVEQAKQEHERTFTRADISKMMTAE
                     RAKWEAEQSDAIEKARTEGERLAKMSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     QTLLVEKGLPLEFIDVVLGTTAEEVKTNIDNLQIIFDKAVEKRVNNRLTQKPPRTGNG
                     SVGMTKAEIMAVEDDDERMRLIAENRNLF"
     gene            complement(17671..17874)
                     /locus_tag="VBC81_000022"
     CDS             complement(17671..17874)
                     /locus_tag="VBC81_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911095.1"
                     /translation="MNKRIKKKRKLERAIVMLIAENAMQAEALRNQHKRIEQLEQIVE
                     HNAQATNNELDYIKNNYKKKWKK"
     gene            complement(17879..18067)
                     /locus_tag="VBC81_000023"
     CDS             complement(17879..18067)
                     /locus_tag="VBC81_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911096.1"
                     /translation="MGVRPPSFTTISIHQYSRIIKEEDMFIWRMVLVALGVLVLILII
                     GIAAIAVKSIIAEVKKEG"
     gene            complement(18095..19543)
                     /locus_tag="VBC81_000024"
     CDS             complement(18095..19543)
                     /locus_tag="VBC81_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEO6911097.1"
                     /translation="MLMDKKNQQQDHLDYWQKRQEDIVSYLDRADLDIFNELSRVYSN
                     QSLSLQKDLYDFYNRFAEDNKITYQDAVKKLRHEDLSDYAANANRYREQAEKDPELLK
                     RINEQYVSAQATRLDMLNLELIHRAGTMRGILDGLFSDHLRKAASYAYQKSVGGRSGT
                     INGPALEQLIKTPFDGYNYSEQLWGNVDNLVKDLQEAFKKGFVRGLGPREMARELGKR
                     YDVARHRAETLIRTDGTMIINNATAKRYIDAGIKYYRDLVQLDDRTTQICRSKARENK
                     KKLLSEIQPGINAAPYHFNCRTTIIPDEDELNTDVEPIADKSTKYFKDVTSDWIDGQE
                     YESQISMLNEYTKGGGTYRVDGHNVVLDHSNYEYRVANWLSKKTGLQVEMVPRVNFPE
                     NINTPDYLVDDVPFDLKEITGSGKNVIDGNLRKAKKQAPNIIFDITKTPLSFEEILGQ
                     LEHIYKIDRRGLNISIIKNKDEVLAVVEKERR"
     gene            complement(19503..21032)
                     /locus_tag="VBC81_000025"
     CDS             complement(19503..21032)
                     /locus_tag="VBC81_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6911098.1"
                     /translation="MFERKFLSGTRFNENANRQFIMLQEDFDAIEYNSQKWIDQLKKY
                     ISSFETEQLDRLKELKRYYLGDNNIKYRPAKTDEYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNEDSDIQKNINLMSVRNNEDYHNVAIKTDLSIYGRAYELLTVEEVDNGTEI
                     KLYQLPAEQTFVIYDDTYQHNSLMAVHFYDIDYGAGKRKQIVKVYTSNMIFTYEDFNQ
                     ETKGMSLKDEEEHYFSGVPVNEYSNNDDRAGAYESVLDNIDAYDLSQSELANFQQDSV
                     DAILVLSGNPYTGADENDFFEDGRINPNGRLGISIGFKKAKVIVLDDNPNPDGAKPDA
                     YYLKKEYDMAGSEAYKNRLVSDILRFTFTPDIQDLKFSGVQSGESMKYKLMASDNYRE
                     KQERLFKKGLMRRLRLAANIWSIKGNESTVYSQVNDTSIVFTPNVPQNDNEIVSAAQS
                     LYGIVSDQTIFEILNAVTGVDAEAELKRLKEEADEKQVMPEPRNVGDANGQEEPTAGP
                     S"
     gene            complement(21047..22327)
                     /locus_tag="VBC81_000026"
     CDS             complement(21047..22327)
                     /locus_tag="VBC81_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6911099.1"
                     /translation="MTTKQRPKINIVIKYPSKVFNKHIYDKLYNYDNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAN
                     RNEAYYKIYALGQFATLEKLIFPKYEKRILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKSLGYAKEEIRGDSAEKKSNQELRNLGIQ
                     RMIDVKKGAGSVMQGIQYMLQYDIVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSSDRNKRLNNAKYYFR"
     gene            complement(22317..22748)
                     /locus_tag="VBC81_000027"
     CDS             complement(22317..22748)
                     /locus_tag="VBC81_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6911100.1"
                     /translation="MSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARSIGQENLT
                     KPDIKAYIDERIEKLESAKIATQEEVLQYLTSIMRGDQQEKTLISIGELGQEIVDIDV
                     GAKDRIKAAELLGKRYRLFTDKIEMDISSDVTISVGEWDDD"
     gene            complement(22869..23282)
                     /locus_tag="VBC81_000028"
     CDS             complement(22869..23282)
                     /locus_tag="VBC81_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6911101.1"
                     /translation="MSKLSNAQLKAFDEWLFDYRDIERKIAIRKLELQTPVSTDINVG
                     GGKSNLVAKETEDIVIKWSKDGKIKSYENFRESVDRVKALLDDELNYIFDLRWGTGSN
                     NTWEEIAYKIHTSRAGTYRKRDRILTLFAQQIGKL"
     gene            complement(23360..23692)
                     /locus_tag="VBC81_000029"
     CDS             complement(23360..23692)
                     /locus_tag="VBC81_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911102.1"
                     /translation="MSLIDEVKECGSESHKKWFDRYFDGLKIEEKLKQSAMKGFSGYK
                     ISIPKNDEYLARRLDSQKTIDLLKYRLGDGFDVEIKNLESSYKIFGKPVVLSRDLFIF
                     WRTGDKNG"
     gene            complement(23695..23925)
                     /locus_tag="VBC81_000030"
     CDS             complement(23695..23925)
                     /locus_tag="VBC81_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911103.1"
                     /translation="MNEQNNKLHKQSIVIVVLTVAVSVLLTTNIALYNYYQPQITGLQ
                     QQLIRTQYRLKESSEQNQRQTKRIAELTGNGG"
     gene            complement(23922..24158)
                     /locus_tag="VBC81_000031"
     CDS             complement(23922..24158)
                     /locus_tag="VBC81_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6911104.1"
                     /translation="MTGELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLVEYWLLESVNE"
     gene            complement(24155..24370)
                     /locus_tag="VBC81_000032"
     CDS             complement(24155..24370)
                     /locus_tag="VBC81_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911105.1"
                     /translation="MKIDYIDFFERVVPGWMRESNKKMQELGFGTEQYWVWVNMSIVD
                     ICESYGNDDLVNGQFHMIWEWLERKTK"
     gene            complement(24367..24765)
                     /locus_tag="VBC81_000033"
     CDS             complement(24367..24765)
                     /locus_tag="VBC81_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO6911106.1"
                     /translation="MTIEFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMF
                     MDKFAQHVPKEKLNGPIRLTTKWLFPKIKKSVNGQYKDTKPDTDNLNKLPKDCMTELG
                     FWNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(24792..24989)
                     /locus_tag="VBC81_000034"
     CDS             complement(24792..24989)
                     /locus_tag="VBC81_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911107.1"
                     /translation="MKEYKKRMIVKYQELKSRAEKLSVLINKFYIDSLDFKLQCPIEL
                     LVAQWHAMGAYLKILEQRAAI"
     gene            complement(25359..27638)
                     /locus_tag="VBC81_000035"
     CDS             complement(25359..27638)
                     /locus_tag="VBC81_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6911108.1"
                     /translation="MAENDFNLLPLLDYINPATVDYQTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTITGATITQLAKDNGWTSSEYRNSDDAHELSW
                     DDTIDRDYKIVDKNWIESKEIQEPRNWNPVQDLITYIDTLFESTDKVGYVTETYPITL
                     DTGEIVYKPTKGAYDRTAGQLIESLQKNPTDLGAVFGDFKEEAGAWIRFNPLDGNGVK
                     NDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEY
                     RKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGVTRNGHKQFLIDTNVGKTNYEEWYQ
                     WIEDLNDDLPDPETLADEWDNMPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELS
                     IALAEGKEWLGWQCEQGKVLYVNLELDRPSALHRFRDVYEAMSLPPANIKNIDIWNLR
                     GKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATEL
                     GCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEELIKSRSEKAA
                     AKIYQQALQEKALAYYQQEVTLDDLESRYQMQQHFDKAIKDIMIKQPYLEAVKKAQYE
                     VEISTAWRVEGTLREFAKFQPVNMWFSYPVHDVDTTGVLADISLEDNVPTWKKNFEKK
                     SPRESREKKSQKVETAINSLNDGIEPVTIDNLIEYFSTEDKPVSEKTIRRWIKENGKF
                     EVKNKQILPIEEPKTNKSL"
     gene            27709..27960
                     /locus_tag="VBC81_000036"
     CDS             27709..27960
                     /locus_tag="VBC81_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911109.1"
                     /translation="MPEIKITFSDGSIVIFHEEQSFQSVVKSNDAMSLSKIYSLWNHV
                     HDGLIPSFLELIANSQFFFDLENPQIYYASNAVIKIEAI"
     gene            complement(27953..28150)
                     /locus_tag="VBC81_000037"
     CDS             complement(27953..28150)
                     /locus_tag="VBC81_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911110.1"
                     /translation="MENINEQIEKFISNFADEAIEKSETYSEAILYVDKHSSLTEFGQ
                     VVKKAIQEKIRDIALNSRIIK"
     gene            complement(28159..29742)
                     /locus_tag="VBC81_000038"
     CDS             complement(28159..29742)
                     /locus_tag="VBC81_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6911111.1"
                     /translation="MKLREYQEEARSAVQQEWEQGKKKTLLVLPTGCGKTIVFSKIIE
                     DRVKLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAESTSLGSWYRVVVGSVQTL
                     QREKRLSKFPTDYFDTIVVDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCSNRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKEKLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVENMTDETEVAFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPASEKQLKTLEKFGI
                     FTDEIGNAGKAAKLLDRLDKRRQSGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            complement(29748..30356)
                     /locus_tag="VBC81_000039"
     CDS             complement(29748..30356)
                     /locus_tag="VBC81_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911112.1"
                     /translation="MGKAKDYTGQRFGRLVVIERLPQDPYTKAKYKCQCDCGKLTIVN
                     SSNLATGHSTSCGCIVTKHGFARKERLYNIWVGMRQRCRDKNSNDYIKYGGRGITICD
                     EWEEYIQFKQWASSNGYRDNLSIDRIDVNGNYEPSNCRWANSTEQANNQRNNHLITFK
                     GKTQTIKQWSSELGISNITLLTRLNRGWSITKALTTPTQKGV"
     gene            complement(30356..30961)
                     /locus_tag="VBC81_000040"
     CDS             complement(30356..30961)
                     /locus_tag="VBC81_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911113.1"
                     /translation="MTEYNNNFEREFGWDDTIQEDAKEFITLQPGDYVFTVTDFERAR
                     HTPNPQKTGKLPACNKAVISLEIETDEGVASLKHNLFLHSSTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGATGVCSVKNRTYNDNVYNEVKSMIYADSVDWTKVLNANTQPQAPQ
                     PTYQQPAQQTTNFAPQQQPAPQSYQQGQMQASQQQGGWGGF"
     gene            complement(31057..32130)
                     /locus_tag="VBC81_000041"
     CDS             complement(31057..32130)
                     /locus_tag="VBC81_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6911114.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAFVKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTADNNKKKATGGTRVLYTQHHPAWDAK
                     NRHGLQEEMPLDYAGIAHIFAQAPSQPVAETPPVQQPVPTQPIHEPVHEQQAQMSGPT
                     AATEYPSVLPKALTDLMTPEQVTPDELVAVANIRGHFPPLTPIENFPPDYWNMIVANW
                     GATLEVIKTQVRATEQPFTVEGS"
     gene            complement(32145..33464)
                     /locus_tag="VBC81_000042"
     CDS             complement(32145..33464)
                     /locus_tag="VBC81_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6911115.1"
                     /translation="MTITINKLEIENVKRIKAVKVEPSSKGLTIIGGNNNQGKTSVLD
                     SIAWVLGGNKFKPSQAAREGTMVPPTLKITMSNGLIVERKGKNSSLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELELKEKELYNQRHTIGV
                     IADQKEKFAKEQPYYNDAPKELISIADLIQQQQEVLAKNGENARKRQNLSVIQQNHQF
                     KQSEVEHLKQQLATAEKQLQELSSDLEIAQRDTMSLIDESTAEIEENISNIEEVNRKV
                     RANLDKDKAEEDAKHQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKAHSFKTAEGGSFAETVAPTWQGGF"
     gene            complement(33448..33678)
                     /locus_tag="VBC81_000043"
     CDS             complement(33448..33678)
                     /locus_tag="VBC81_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911116.1"
                     /translation="MIEVERQPKNTYSRIRCSNNIYDEIANIANECDLTLKEVTDALL
                     NYGLANAQIISSEKVVTESKLVIGDFDNDNHN"
     gene            complement(33671..33898)
                     /locus_tag="VBC81_000044"
     CDS             complement(33671..33898)
                     /locus_tag="VBC81_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911117.1"
                     /translation="MLKEVLLENELLRDENRRLTNEMADYYFTNVAKANLLDIIVNEG
                     YILDSTLEKCIAKLDNVDRQTLEKNWSDDND"
     gene            complement(33892..34041)
                     /locus_tag="VBC81_000045"
     CDS             complement(33892..34041)
                     /locus_tag="VBC81_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911118.1"
                     /translation="MKKLFKKLFVKKNQVIETRQKWTIETHGWEANARRYDNMIERNN
                     RGRTC"
     gene            complement(34046..34357)
                     /locus_tag="VBC81_000046"
     CDS             complement(34046..34357)
                     /locus_tag="VBC81_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6911119.1"
                     /translation="MPKAELVYRPAKQSEKADYGDYVHLCQIWEGLTVGTAKVWAAEM
                     RNHPDFKQFINNPTHRIVFINYEGFKLFVTWKSRNRYKPKKETLAEMLENIKKERQLG
                     V"
     gene            complement(34437..34628)
                     /locus_tag="VBC81_000047"
     CDS             complement(34437..34628)
                     /locus_tag="VBC81_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6911120.1"
                     /translation="MPDIAVGREKVVTFLKSNNIKKSDLAAAYGLNRQEVTNILSGST
                     RGPKANQFILRVIADYSIE"
     gene            34679..35467
                     /locus_tag="VBC81_000048"
     CDS             34679..35467
                     /locus_tag="VBC81_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911121.1"
                     /translation="MSRYSDYKYANTILKSIEERPEKYLIIHYSCESFYNLGSKSPRI
                     ASISVRQFNNAQTNNFSIHQYSEILDTPITDDTYETIEKELLTDFFTFVDRNTDKNWI
                     HWNMRDTVFGFHAIEQRFKVLGGTPIIIDDDKKIDLAYLFKKMYGGGYIDNPHIEKLL
                     ELNNLTPHRFLTGKQEADAFSSRQYHELSMSTSSKVNSFSTFLTLAINDDLKTNTPKW
                     KMRGTNLSGLLSAFQETTSGKVIIGIINLIVGGIVGAIIAKYIQ"
     gene            complement(35418..35663)
                     /locus_tag="VBC81_000049"
     CDS             complement(35418..35663)
                     /locus_tag="VBC81_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911122.1"
                     /translation="MKKNIVDLQKRNEHFQWVADSLEGKENELYVERDWYDNPTLISK
                     EDAKKEVEQVQQKLILLQKKSFIEYILQLLHQLFRRQ"
     gene            35959..36306
                     /locus_tag="VBC81_000050"
     CDS             35959..36306
                     /locus_tag="VBC81_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911123.1"
                     /translation="MFPTFEKIKELCKQRGITLIQLEETLGYSRNTLYKLKTQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPNIAKDSQEYTSDDLRKMAEKAKTFDGKPLTESDID
                     AIQNIIEIYLKGR"
     gene            36310..36696
                     /locus_tag="VBC81_000051"
     CDS             36310..36696
                     /locus_tag="VBC81_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6911124.1"
                     /translation="MTIEEIVDCHKVNLAYFDNELWPRPGIYIDDIKVIFVNKSLSLD
                     AQKRVILHELGHCERNIGDYKTLREKHELEADRYMISHLIAEALSQLEDKREFNYLQF
                     MQYHQLTTTADECMVIDEYYNLLKVV"
     gene            36714..37265
                     /locus_tag="VBC81_000052"
     CDS             36714..37265
                     /locus_tag="VBC81_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911125.1"
                     /translation="MDINKIKEFAVSTSEKAKDGIIKANEMRKRASQESKIVLPPTIN
                     LGFNTPTAIRKTVEGDYYIGFYSEKPDLFKFVDFQFDGSRIIEKTVTKGKTTQKGRSG
                     STIIGGLIGATINPTAAVLGGLAGANRGKKGTINSTSTTITEEIPGKAQLTLRNLKTN
                     DIKIIKTKLTQAQANNIQNFFSK"
     gene            37380..38522
                     /locus_tag="VBC81_000053"
     CDS             37380..38522
                     /locus_tag="VBC81_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6911126.1"
                     /translation="MWSEKHKSGKVNFVERYKDPYTNKWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTNLIDEWWIFYQQEIKRSSIVTLKGNIREIRAEFGIN
                     IPVVNIDPRYVQNYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPKI
                     KKTLNDWKKIEEKYLEEEEIKRLLKELFRRPSTHRLGFLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYINGEKTSPKTLASYRETIMSKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTRNGVPIQINSFNLALKKANERLEQPINKNITSHIFRHTLVS
                     RLAENNVPLKAIMDRVGHADAKTTVQIYTHITKKMKSNIADIMENY"
     gene            complement(38610..38885)
                     /locus_tag="VBC81_000054"
     CDS             complement(38610..38885)
                     /locus_tag="VBC81_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6911127.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(38988..39590)
                     /locus_tag="VBC81_000055"
     CDS             complement(38988..39590)
                     /locus_tag="VBC81_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6911128.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            complement(39565..40404)
                     /locus_tag="VBC81_000056"
     CDS             complement(39565..40404)
                     /locus_tag="VBC81_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6911129.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(40397..41233)
                     /locus_tag="VBC81_000057"
     CDS             complement(40397..41233)
                     /locus_tag="VBC81_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6911130.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(41346..43004)
                     /gene="recN"
                     /locus_tag="VBC81_000058"
     CDS             complement(41346..43004)
                     /gene="recN"
                     /locus_tag="VBC81_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6911131.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(43016..43489)
                     /locus_tag="VBC81_000059"
     CDS             complement(43016..43489)
                     /locus_tag="VBC81_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6911132.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(43476..44303)
                     /locus_tag="VBC81_000060"
     CDS             complement(43476..44303)
                     /locus_tag="VBC81_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO6911133.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(44296..45168)
                     /locus_tag="VBC81_000061"
     CDS             complement(44296..45168)
                     /locus_tag="VBC81_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6911134.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(45168..45383)
                     /locus_tag="VBC81_000062"
     CDS             complement(45168..45383)
                     /locus_tag="VBC81_000062"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6911135.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(45361..46701)
                     /locus_tag="VBC81_000063"
     CDS             complement(45361..46701)
                     /locus_tag="VBC81_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6911136.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(46827..47663)
                     /locus_tag="VBC81_000064"
     CDS             complement(46827..47663)
                     /locus_tag="VBC81_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6911137.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(47660..48514)
                     /locus_tag="VBC81_000065"
     CDS             complement(47660..48514)
                     /locus_tag="VBC81_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6911138.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(48653..50347)
                     /locus_tag="VBC81_000066"
     CDS             complement(48653..50347)
                     /locus_tag="VBC81_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6911139.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(50634..51368)
                     /locus_tag="VBC81_000067"
     CDS             complement(50634..51368)
                     /locus_tag="VBC81_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6911140.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(51361..52053)
                     /locus_tag="VBC81_000068"
     CDS             complement(51361..52053)
                     /locus_tag="VBC81_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6911141.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
     gene            complement(52194..52424)
                     /locus_tag="VBC81_000069"
     CDS             complement(52194..52424)
                     /locus_tag="VBC81_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6911142.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            52722..54983
                     /locus_tag="VBC81_000070"
     CDS             52722..54983
                     /locus_tag="VBC81_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6911143.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            55169..55624
                     /locus_tag="VBC81_000071"
     CDS             55169..55624
                     /locus_tag="VBC81_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6911144.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            55688..55990
                     /locus_tag="VBC81_000072"
     CDS             55688..55990
                     /locus_tag="VBC81_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6911145.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(56060..58852)
                     /locus_tag="VBC81_000073"
     CDS             complement(56060..58852)
                     /locus_tag="VBC81_000073"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6911146.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(59137..59907)
                     /locus_tag="VBC81_000074"
     CDS             complement(59137..59907)
                     /locus_tag="VBC81_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6911147.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(59917..60705)
                     /locus_tag="VBC81_000075"
     CDS             complement(59917..60705)
                     /locus_tag="VBC81_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6911148.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(60707..60961)
                     /locus_tag="VBC81_000076"
     CDS             complement(60707..60961)
                     /locus_tag="VBC81_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6911149.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(60964..61569)
                     /locus_tag="VBC81_000077"
     CDS             complement(60964..61569)
                     /locus_tag="VBC81_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6911150.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(61581..62255)
                     /locus_tag="VBC81_000078"
     CDS             complement(61581..62255)
                     /locus_tag="VBC81_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6911151.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(62261..63541)
                     /gene="ftsZ"
                     /locus_tag="VBC81_000079"
     CDS             complement(62261..63541)
                     /gene="ftsZ"
                     /locus_tag="VBC81_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6911152.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(63563..64936)
                     /gene="ftsA"
                     /locus_tag="VBC81_000080"
     CDS             complement(63563..64936)
                     /gene="ftsA"
                     /locus_tag="VBC81_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6911153.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(65209..>65454)
                     /locus_tag="VBC81_000081"
     CDS             complement(65209..>65454)
                     /locus_tag="VBC81_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEO6911154.1"
                     /translation="IKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNKSTDKTQT
                     QNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
BASE COUNT        19530 a        13267 c        10362 g        22295 t
ORIGIN      
        1 gatttgctta cttcaatcgg aactctgttg gttggtatag caagcataat cacagcaata
       61 aaaaaagaac ctaaaaagaa aaaccggcca cggagattca aataaggttc tagtagtagt
      121 ttggggctca agccccttgc cactactgat agtatatcat atctaagaca aatatgaaat
      181 atttgattat tttcgcgatt tgtttagttg tattttactt tattaataag gatgattgaa
      241 atggataaag aattaacacc tcaagaaaaa gcaaataaaa agtgggcaga aaacaataga
      301 gaacatagaa cctatctatc aaaacgatct actgctcgta gttttattaa caaaaatgct
      361 acaaaagaag acttattaga attaaaacaa ttaattgaaa gcaaactcta gacacacaaa
      421 aaaccgccct caattagaga gcggttaata tttatttcag tttttctttg acagcatcta
      481 ctgcctcttc aacagcatct ttagcatcat ctgctagttc tttgccttta gcaattgttt
      541 tttcgacaaa tccttttgct tctaactctt tatcaccggt tagcttccct gcaccttctt
      601 taagactgcc tgacgcttgt tcaacttttg cttttagttt ttcttgtgac ataatgtggc
      661 tccttattat ttttattcta tggtaacatt ttgattattt ctaggcaaat aaaaaagcaa
      721 gaaccgctag tgtcaggcga ttcttgctag tgtgattatc tcatgattat gcgagggtgt
      781 caataagggg aagagaaatc caagcgtcag cctctaaagt ttttcttttt tttgatttcc
      841 tttatagtgt tcattgatgc aaagccataa tattttgctg tatcctcgtc cttaataagt
      901 ttatgcaaag aatcaatgat atctcgaaaa tggtatttag gatttatttt acccatcatt
      961 tccaagacaa ttaagaaagg tagagcaatc ctgtttgaaa aaacaccttc tttatactca
     1021 aacaaaaaat ctttccactc ttcttttaac ttagagacag ttataaattt aaaatctatg
     1081 atatttgaat tgtgggcgca aatattacgg gctagattaa tacattttaa ccaagaaatt
     1141 aattcaggat tactacatga aaaagtgctt gaaatttgag ttaaattagt tgttgacatc
     1201 aattctagca aattaaccat ctgcccaaat gttaacatat ttaccgctag ccaaatagga
     1261 ggatattttt gtctgtctag ttttaatttt tcatccaatt cagaggatga tgcttttctt
     1321 agctctcttt ttaactgttt tttaaagtta ttttcactat aggaaaggta gtgtttgcaa
     1381 tactcttctt tgttacacca cttagaaaaa tctagatagc catagcttcc taagccgttt
     1441 ttccccagaa cataggcgat ttttgttttt attgctactt caatatcttc aatcgcatgg
     1501 agtaaattta atctcaaatt cttatcctga taatatctag atataactat ctcaaatttc
     1561 gtaccctgat aatctatttt tttcttttgt ccatcttttt gaatttttgc aaaaggcttt
     1621 gcaaattctt ttattttgta gtaagaaatc acagataaac tatactctgc tttactttta
     1681 gcttttcttc cactaaagac aatacctcta gattccaaaa gttcaacttg ttctctgtaa
     1741 cttttgtgtt gaaaattttc cgccatgaaa caccctcaat tttttagcaa aaaaaccccc
     1801 catcagaaca tgtctgcgcg tatgcgaggg aggccattga tatttatata tacattatat
     1861 atttttatat agcttattgt caatgatttt ccctttatat aattaaactt tttaataaaa
     1921 tatatttttc atccagtaaa aggcacctaa atacctcctg aaaaatctgt tcaaaataat
     1981 aaacagcccc cgcaaaagcg agggcatttg tcttatctaa aggaatttta cctcctaaat
     2041 tgttttttta gttgcggtgt aacttacacc attaacagac cgacatttat atcgctctgt
     2101 tggtttacat atctgttgca tcaattaagt aagcatcttc tacccactga ttagactgtg
     2161 gagcgcctat ccttgcccag ccttttactt tttcgtaaac tcgaactcta gtaccagcaa
     2221 caagcaactc cttatcagtg ctattgacgt caggcttgga ctcaacgtaa taatcttctg
     2281 aaattgttgc ttcgtaatat ggcatatttg aattacttag cggtgtgtta acgtctaact
     2341 ctttttcaaa tttagataca actgatttat tatcaacctt agcatttgac tgtttcccag
     2401 agtaacgata agcgtaaaca tacggttgac cattatatcc ccagatttca tcatggttat
     2461 tcactgtaat tgagttataa ccataattac agtgaataat attgtctgga tcaacaaaca
     2521 taccagtatg tccaaaagct ccagccgaag ccccacgttt accccaaata aaaatatcac
     2581 ctctttgcgc attccaatta gtattttctg caataagaac ataaccgttt tttatcaacc
     2641 aatcgtgctc atattctgta tttactgccc agccattatc tgatgcgcct gctgagcgta
     2701 gagcaaaata gacagagcta gagcaatcgt aagatgaagg accatttcga tagtccatcg
     2761 agtaagtaac tttacctttt ctagatgcca tccatgcgat agcttgctca atattaatta
     2821 ccatattatt gacctttctt ccactcatca ttcatgcgtt taaccgcagc ttcgataaaa
     2881 gtttcaagtt gtgtctgtgt aagattaata ttatattgcg ataaaccatc aataacagcg
     2941 gttttagctt ctgttagctt atcttgtccc ttgataccac caacttccgc agatatttgt
     3001 tcaactgcct caacagcatt acgagcgaca atttctgcga ttttaacagc tttttctcca
     3061 ccttttttta taagtaattt ttttacttcg tgcgtgagaa tacctgcgat gataccaaaa
     3121 attggtactg atactgtaat gatttgtgtt gtaaattcgt tcatgttatt tctccttatt
     3181 attattaaat aatgttttga tttgctcttt attgatgatg atatcatcct cgacacgacc
     3241 taaacgctcc tcgtgacgat caataacagc ttttgtaatc tctcggtctc tatctatatt
     3301 tttgagctcg tatgctagtt ctttgagtga gtccttgagc tgagccatag caatctcgtt
     3361 agcttccatt gcttttttaa agggattaac aataaatccc catactccca aaatagataa
     3421 agcagcacct gcaaaagcgc caacttgcac aaaatctatc attaagttac ctcactctga
     3481 ttctttatca gtaacttttt gactaagtaa ctcaagcaca acatctcgta aattaaataa
     3541 tttaggtact gcttcgaaat cacaaatacc attcgcaaca cgacggtacc aaacgtcaac
     3601 aataagatga tcttttttaa aagtatatgt catattaaat tctccttttt ttattaaata
     3661 atatagtgtt ataacggtat tcaacgttaa ttccattagc ttgattccat cgatgtagac
     3721 ggcacggaaa ggctagatac acttgatgtc actaagtcag ttaactcgac aactgcttta
     3781 tcaactttaa gctgcatatc ttttgttgct tgcgacatct tagaaattgc ctcatcagcc
     3841 tctgcaattt ttttgattaa agttactttg tcagctttta gttgctcaat atcttttaca
     3901 gactcaagca ttgcgaaatc aacgacattt tcttttgcaa aagtttcaag tacaagtctt
     3961 atcagtgatt gattatcctt atcaacatga ttaccaatta gctcttgtgg taaatatgta
     4021 ccatcgtcgc cttgtaagcg tacatccgtt tttacaacca ctccatcttg atagattgga
     4081 aatggttttc caacaatgtt ccattgtttc atgttactca cctcactttg ctcctggctt
     4141 agttatctca tctaattgct gatttaactc agcaatttgt aattgtaatt gctcattttg
     4201 agcttgtaga gttgctttat ctaaagataa ttgcgctatt tgtaaagcta aatttgattt
     4261 tattttttct tccatttttt ctcctaaatc tttcctggaa tagtataact gtagcctttt
     4321 tcagtcgttt tattattgtg tagtagctgc aagttatcaa taatcatatt aattgtatct
     4381 ttcatgcttg tataagttga tgtatttttc catagccaaa tatctcctac ttttatctta
     4441 gacgttacac gatgatttat gttacgtgca tctattgata gctgatttgg caaagtaatg
     4501 atttcccaac catcagcgtt agtgtacgca gagcttgcta agtgaatact atcgccaact
     4561 aaatcaagag tatcgatgtc tttaccattc caagcgcgaa tacctacgaa accaccgtcg
     4621 tttgacgatt cacttcccca tcgattagaa cctataaccg ttacgccagc cgcgcccttt
     4681 ccttcaacat tacctgttgc aaatttaacg aattggttag gataacctgc agtaatacgt
     4741 ttaatagctg gattatctgt gtaaaattca atttgtcctg tattaagatc ggttaacatt
     4801 gcaccgtttt gcgatattaa aacgccgcta cgtatcaggt ccgctgacat tcttccgctg
     4861 gtaatattac tagcatctaa attaatgaca tttatacgat tagcatcaat tgttcctgct
     4921 gtaatcttat ctgcagttaa atcttttatc atcgcattag taataatgcc attatcaata
     4981 atagattgac ctgacaaatg tattaactta ccttccagtt ttacgttacc atcagtcaaa
     5041 ttaagctgac tcaatacagt accgttagaa gtcaaatttt taatagccca actgttcgct
     5101 gtttgtgcga ctattgtatt aatcgcagta acattatctt tgatatcttg cttggattca
     5161 gaccagggta gatcagcagt acctttagat aacataaaac caccaaccat aaaccagcct
     5221 ggatcactgg ccaacattgc gaaccgtggt ctgattttac cgtctcgagt tggaataaat
     5281 gttattttaa atcgtctaat ggtttgagtg acgtctttaa taatagtttc acgaggagtt
     5341 gtgcttgtaa tatgatgatt ccaaagatca tacaagtaga aatatagatt tcctgcttgt
     5401 tctctagaaa tgtatgcagt aaacgtatac tcttgatctt ttttgacatc atattcgatt
     5461 aaaggtgaca ccttattacc gcttacccat tttttaaaaa caaatgggta aggcgattgt
     5521 gttagatcct ctagtactgc accttctgtt tgccatcctg tgaatttttt tgtgccaggt
     5581 aaaatgttgt tatcaagctg actaactgtt gcaactatac cgtcagccgt ttgggtaact
     5641 cgtgacaagc gtccatccac attagatact gttgattgta tattatcaac tttttgtatt
     5701 gtagctgtca gactctcgtt ttgtttacca atcagttgac tatgactatt aactgtatcg
     5761 ttgattttat taaattcaac agtcatttta tcttcttgat ccgctgggga ctctgaatag
     5821 tcacttgtaa catttccatg ttcaagctgg taacctgcaa tatacaacca accattttca
     5881 ttaaagcgtt cgaagcgtgg taaaatatag ccatcagaag ttacgttaaa tttaataacg
     5941 atacgttgcc aatccgtgtt taaagtgatc gacttgaaac taatacttat cttagcctgt
     6001 tgcgattgat catttggtaa caaatataaa ttaacaacat cattatctat gcttgattta
     6061 gcatatgcac taaaaacgta attctctccg ctttttacag catacaattg tgatatacca
     6121 ctccacattg ctttgcgctt tagcactgtt aagctaaaat taccaatagt caagttatcg
     6181 tcatcagatt gccagttatt acgattaaac caatttttag tccaattttt agtaccagat
     6241 agtagattaa ctgtacctat ctttaaatta tcaatagtat tattaacacg agttatttct
     6301 ctgctaaaac tatctgcagt ttccttgact ttattatcag cgataacagc agacttactt
     6361 gtaatcaacg cttcaatatt tgtttgagat aatctcgttg taatttcatt agccgtttgt
     6421 gtgacttggg tctgtagact gctaagcgta ccatcaacag tctttttgtt agcagtaata
     6481 cttgtactat tttgatcaat cgtctgctta taattagcga gtgtttgagt gatatctcca
     6541 tcttttttta acaatgcctc gtagctagtt tgtaatcctt tgactgtacc atcaagttgt
     6601 gtcagttgca gattagttgt tgaataatgc tcaacttctg ttttagataa cgcagacatt
     6661 ttatccgtca gttttttaga ttcactaata aggtttgtca atgcttctgt agcatctttt
     6721 ttggcagaat cagacaaatt ctttgcttga ttaattaatt cgtcttggct aacattttta
     6781 gcaacaattg cattatattt ttcagttaac tgttcatcag caagtttcat tgctgcatta
     6841 acctctatga ttttattagt aatttctttc tttacaaaat caatatcgtc gctaaaagaa
     6901 ttgataatcc attcagcacc attccagaaa tacattctgg tagtatcgcc aacagttaaa
     6961 aaaagcaaat caccctttct tagcgtgcct tttggatcat ctactggcat ctccgtacca
     7021 tagtagttag tgtttttacc atcagctgat gctagtgctt gatttgcttt aaatactgca
     7081 ttatctagta tttcttggtt attattaatc ttacttgata attggctaac agctacagtt
     7141 tgttttttga ttgtaccaat atcgttacaa gtgactttat ggttaatcag ctgacctgtt
     7201 acatcatact cactttcaaa cgatactatt cggatttttt ccttaaatcc tagtgtctca
     7261 ttgatagcca taatataatc accggcaata ggttgagtgt actgataacc tgcttttgta
     7321 agatcttcaa tgtcaatttt tacagcgata ttgtaagacg aatctacatt ttgttttagt
     7381 actgatttta aattgtcagc gttagtgtac cgctcatcaa caacaggctc accctcaatt
     7441 cgtccatatt cctttgcaag tggactttca tattctactt cgagccttcc tttggaatga
     7501 tcctcttggt cagtccacgc tccaaaaccc ttctgatatg taataaaatc attgattttc
     7561 ttttcaatta tgatttcatt catattgaaa tttttacgta cgattgttga taaatctgtt
     7621 cctgtttttt gtagaattcg aacaaccttg ccacgaacaa aaaattcaac tcctgcactc
     7681 ttaatgatgt cattaaataa atcaagtcgc gatttatatc caaacgattg cttttcgaaa
     7741 gatttgacaa ctacttctaa actgtaagta tacccgctgc cattaaaaat aaagttcaaa
     7801 tatgcttgga aagtatgtga accatcattt aattgcttat gtagtgatga tttatcaaaa
     7861 tcataaaaaa attgatgaac tgcatcaaat tcaacttgaa ttttgttacc agtatcagta
     7921 ggtttagcat aaataatctt gtaatactca tcatcaaact caagtttcca acctttatcg
     7981 accttgttta gtacctcttc gttagtataa attgttcctg ataaagacct ttctccgtta
     8041 actgcattag ttgttttaac aacagcttga gcttcaaact cattatcttt atgatctaaa
     8101 aaagttgtca atctatcact tccttacttg taaagttctt taaagtttaa aaacttgatt
     8161 gtgccttgaa aatttgtgct gtagagaata gtcttgttga ctttttgctt taaaacaaaa
     8221 tattcatgat tggttctagc agtcacattt atcttattca atgttgtgga tataccagtg
     8281 attacaataa cgtcgccttg attaagtggt gcactatatt gataagccca gcgcctccca
     8341 tcgatttcaa gataaaaact actttgtcca ccagttgaag ttaactctac agaccatggg
     8401 tattctaact ggctaaaagg tgcagtacca gaataattaa acgttccgtt agtaatgact
     8461 atattttgtg caatagtttc accaaatgga agttctgcag tctcaaatgc aattgtaaag
     8521 ttgtatttga gaccttcact agacttacca acaaaagtgt actcaggttc acttgagaca
     8581 taaactttcc aacgatagtg ccacgctgta tgagattgat ttaataagtc taaatcacct
     8641 gctttcatcc caggcaattc gaaatcatat aaattttctt tctgaggata cattttagta
     8701 atgtaaaaag gctcactatc cactagtaag ccattcacat catctttttt tgtcataaaa
     8761 tctttaatag aagctacaac taagcgccca ttaaccttaa ctttttttgt gccatactta
     8821 gcactagcaa agattttacc gcttcgacct ttcactgttc gactatcaac ctcaagagat
     8881 gatgaacggt catcgacatc aagtattcgt agaccaatct ctgaaaatcg aaaggattga
     8941 tcactctttt gtactaataa atccattttt ctcctctctt atgtcgcaaa gttgaagtaa
     9001 ttatctttat tactttctct tgcttcttta gatttaatag ttgtataaat ttcatcacca
     9061 acaagttcat tatgaacctc gaacgttgga ctagtcaatt gttggttgcg tacctcgtcg
     9121 cttagattat caagtgatga tgatacacct gcacttgata tgtttgcaga tgtcgctaat
     9181 tgtgaatctg tttcccatcg ctgatcagtc acagcattag cgtactcttt acctattaca
     9241 ttgatatcat ccatccaatc ctgcatacca agtgcaaaac cttcaccagt ataagcacca
     9301 agttctcgcg ttactcgcga aggcgaatga atatccattg cgcttctgat tgttgcagcg
     9361 acattagcag caatactatt agcaatagcc ataatagttc ctgcagtgct tgctaggcca
     9421 tttgcaaatc caatcccagt attataacct gcagatctag caccactcgc tgctgcattc
     9481 attgcagata caaccgaatt cattgcactt cttgttacac tcacagcgct gttcattcca
     9541 cttgaaattg acgaacgaat actagacata gcatttgaaa ctgtagactt agcagaatta
     9601 aaggaacttg taaaggtgga tttaatatta tttccacctg attttacagt attattaata
     9661 ctattcattc cactagtaac agtgctgttc attcctgaca ttgaacttga aactgtagat
     9721 ttagcttgtg taaaactgct attcacactg ttagatatat ttgcaccgcc tgattttgca
     9781 gcatttgcag cttgattcat ccctgtagat acagtgctag ttatacctga catgttatta
     9841 gctacaccac tagccatacc agctgtagca ccagtaactc cagaactcat tccattcgcg
     9901 gaagtggtag catttgcttg tgcagtttgc atattacttg ttgcctgttg cgataattcc
     9961 aagaaattcg gtagagaccc cagactcatt tggctagtag cgttattaac attagcagtc
    10021 atttgattag cttgattagt agcatttgta tttgctaaag tcatgttcga actcaacgct
    10081 tcattaaatc ctgccgcatt cagcaaacct tgatttgcca tagatgctgt ttgtgcattt
    10141 acatttgttg ccatctgagt agcatttgtt gtcgcattta aattagcaag accagtattt
    10201 tgactaatgc tatttagcat agtagttgtg tctatgttag tttgattagc cattgtggtc
    10261 gtctgcatac caacattcat agtcattgtt gacatgtctg atgtaacttt agcggcactt
    10321 gttgagctct ttcctgtgat ttggtcccac atagaactaa atccaccttt aatgccttcc
    10381 catacccctg aaagagcatt cggaatagcc tcaagcatag ctttgccaag tcctataatc
    10441 aattgaatac cagctgaaat aatctgtggc aaccctttaa taattgcaac agccaactgc
    10501 acaactaact gtagccctgc agatataatt tgtggtaatg cttgtgataa accagatatc
    10561 aatgactgaa taatctgcat cgctgcttgg attacctgtg gtaaattttg aataatacct
    10621 tgcactaaca tgattataat ctggatgccg ccttggatta cctgtggcaa atactgagct
    10681 ataccggaaa taaaacctgt gatgacttgg gttgcaattt gtacaactgc gggtaacatt
    10741 tggataatgc cttgcactaa attagttaag atagcaatgc cattattaac aatgcttggc
    10801 atgtttgctt gtaagcttgc cccaaaattg ttaataattt gttgagcata ttgaataatt
    10861 agtggaatat tttgcacaat tccttgaact aaattaacta tcaattccat accaactgct
    10921 aaaagctgag gtaatgcact tgcaattgaa ctaataaatg tccctataat ttgtattgct
    10981 gaactaatta aactcgtagc attttgtcca acaccttgaa ctaaactaga tattaattgg
    11041 acacctgctt gaataataac aggtaacaat acagtaatag catttgcaaa tttagatata
    11101 agttcagcac cactagcaat taattgaggt atttgtgatg taatgccatt taccaaatta
    11161 gtgataattt gagaacctct agtaattgct atatttagca atttatcaat ttgagtacca
    11221 aattgattgt ttactaaacc aagacctgct acgaccaatc ctaaaattgc cgctggccct
    11281 attgctgcca tagcaacgct taccacagct cccatggctt gtgtcattgc accgagtata
    11341 gcaacacctc tcgctgccgc agttgcaaaa atggcaggta aaccacttac tgttgctgca
    11401 aaagcaccaa ttattccgct agcagttgac aatccactgg ctaccattgc tccgaatgat
    11461 cccaacgctg aaccaacagt cccaagagca acaccaatct taccaataat tgatattaaa
    11521 ggcataatag ttgcaatagc acttgcagga tttaataagg cccaagctgc tattgcagta
    11581 ggacctatag atgctatctt tgatttgatt ctatcaatag ttgctgacga tacttcacca
    11641 gtttttgcaa attcacctaa agcagtattt aataatccaa aagctgattt aattccacta
    11701 gttagtggac taaagtcaat attcaccatc ttggagattc tagcgaatgc tgcaacaagg
    11761 tttggcatta ttgagttcac agttgcaaat gcagaattta tagctggctt aatactatta
    11821 atggattctg caatggtctt cataccatta gctttggcag cttcatcaaa ggctgaaatc
    11881 atacctgcta cgccttttac gacggcggta tgtacgttct tccatgcagt tcggattcca
    11941 ccagccgatg attgggccat ctcagcaaaa ccaccctgcg ccttgtttag ttcaatcatt
    12001 ttatcagcga attcttgggc agtaatactg ccgtctgaaa gagctgcttt taagtcctga
    12061 acaccatttt taccaaatcc gaatgactct gccattttag acattaagcc aggggcagct
    12121 tcagacacag aattaaactc ttcggcatat atcttaccag accccaacga ctggttaaac
    12181 tgtctaagtg cttgctcagc cccttcagta gtcgctccat aaccaatcat tgcattatta
    12241 aatgctaatg ctagctctgt tcctttatct aagctacctg tagttatagc taattgttgg
    12301 gcacttttaa cagcactgtc tagtggtgta ggaagtcctt caattccttt agataattta
    12361 tctattgatg ctttagactg ttgtgcagaa tacccaaata gcgccatcgt tttcggaaag
    12421 cggttcatgg tatctacacg acttattgca ccgtccattg ctcctgtgat tgcttggatt
    12481 cctttctgag caatggccat caaactaaaa gcagaaatga tacttcccac agtgcttctt
    12541 agtttttcac cagcagatga tgcgcttgcg aatctctgac taataccatt tagggcacta
    12601 gttgcagaac tggacatcct gctaaaacta ttgctaagac ctgtgcttaa cttagtcgct
    12661 aaattagtaa ccgctgaagt gattctaccc ccaaaactac tactgatttt atctgcaaca
    12721 gtactcgctt tagaactaac agagttgaaa gcagctgaga cggcacttgt gatcttagac
    12781 gaaaagctaa ctactgcact tgttgcactt gtgaatgcgg attttatggg ttgagggatg
    12841 gcgctcgata acttctgtac gctattttga ataattgaaa aagctttatt gaacccattc
    12901 tttatgggtt cgggaatttt ttcaccgata gatgctgcta tcctttgaat ttgaccaaga
    12961 gataacttga gacctgtact atatgcatta ccgagtcttt gccctaatga ttgaccatta
    13021 tttgctaatt gagccataat ttgaccaatc atctgtatca ttttgttgct gttgttaact
    13081 gcagcatcct gcgcttttct aaaagcattt tgtgtagtac taacaattct agccatagct
    13141 gcctcataat ctttagtatt tgcaccaata tcagcgaata tagccccatc aaatgtacct
    13201 gccattaaat ccctcctttc ttgttatatc ttgttataaa ttttgaaaat gtaaattgag
    13261 cttcgctaca cgttctgcga aatcgtttga aatattagtt agatcttgtt tattttgata
    13321 agttcgttta attttgttac gttgtttatc taatttgagc ttattagcat tgacgttttt
    13381 agcatttaac gtgtatctga gatttaaagc aagttcagag aggttttcac gttctgcaat
    13441 ttgcttgtac tgcaaacctt ccataatcgc atctaattcc gttcttgtac acttgtaaat
    13501 aatatcaatg tccgtcaggc caagttgagc acaattaatt aagagattgc gtctttcatt
    13561 ttcccaatca tgtcttttac gatttggatt tgagcttcgt ccgcatcttc tcgagcttct
    13621 aaatatttca ctgatttctc catgctttcg atatatttcg aaatcttctc tttgaaaaaa
    13681 ccagattcaa ccatgtcatt ctcaatttct ttaaataagg cttctgtcga cccttcttct
    13741 tcgactagct ctgcgataga atcaagcact tcatcttctg acaatgcttt tttaccgctg
    13801 cctacagaca gcttgactaa atctactaca gctgaatcat cacgctctaa gattttataa
    13861 aatagcgtac cgacgccgtc agcgttacgt tcccctgtct ctttattcac agtcccaagt
    13921 ctattattta tcttaaacat tgttttaaaa tcaaatttaa tttcaatact gcgttttgct
    13981 actgttaatt ccatttatgt atttctcctt aaaaataaaa ggtagcgaaa tgctacctta
    14041 taaattagtg tccttctgat aatttttcag gaactgctcc cggtgaactt cctgaaactt
    14101 cttttttctg gatattgtca taatcgccag tagtttcgcc tgggttttga tatgcataaa
    14161 cacttgttag caaagcgata tcttctgccg tcagtggaaa tttgccatct tttaaactat
    14221 cgataatatt caatgcatat gatgcttcta caagatcttc aacaccttct gaatattcaa
    14281 tctcgtcaat ttttccatat ccaaacttag caggataaac ttctctatct tttaaagttc
    14341 ctgaaccttt ttctttcaaa gattcgtctg cgataacacg ccaaactttg acagatttac
    14401 cagttgtttt agctatttct acaatttcaa ttgctttgtc tttcggtgtg tagtatgatg
    14461 tcaaatcaat tgagtgctca tctgttgctt tttcaagcac acgtccttgt tgagtttgtt
    14521 catcaataaa atctccaccg agcgtcgttg tcccatctgt gcgatacgct ggcagaattg
    14581 ctttactacc taattcagca tcaacagatt gaataaaata aaatatattt ttacctttaa
    14641 taggcttagc tgtagtaatt tcaactgtag tttccattta attctccttt aaattactat
    14701 ttctgtgata ttgatagcaa tatggtagac ctcacgacct acagaattat ccatcaataa
    14761 ctgtgcatta atccgtttgt ttcgtcctaa ggacataatc attctagact taacctcctc
    14821 tgcatctgtt ctgctagtgg caggtaagaa tgcgtcaata tttagcccaa aatctcccac
    14881 aacagctcct gtctgcgctg tttttgatgt atccgataca ttggaaccta tgacaataaa
    14941 cggctctaaa acgtccgatt ttggtaattt aaaatagatt gggatattaa gtgttcccaa
    15001 tctatttttt acctcattca gatataaagt tgatggtgag tatatcattg atcacctctt
    15061 aaacattttt ttgaggttag ccattaacac tggccattct tttctcaggg cagggtctaa
    15121 aaatggttga gcttccattt tacgagtacc taactcaaga taaatagaat ataaaacagg
    15181 agaaaccact ctatagtgca agagtttttg ttgttcattg tagatttgac ttctcagcca
    15241 tgtagtatca accggggcta gtattttagc ctgtctctca atcctagctg ctgatttgct
    15301 aagtttttta tctacagcga ttcgtgctga cttctgcttg cgctcaacac tgcgtaaaaa
    15361 cctgtccagt cctcgcatac gataagtaag actcatacat aaataaccgt gctattttta
    15421 tggtgcgcta gtccctttat caacctcggt ttccccttgt agataatctt gctaaagcca
    15481 tcgtaatggc cttgcaagtg caacttaaaa gcgttcaaat catatttacc aaataacccc
    15541 atctgttcag attttgtgaa agtattttcc atgcagggga tttttccgct aggtttttca
    15601 acagttcttt tatctagaaa atcgtcctgc tcatcgacat acactaagat aactctatct
    15661 ttgtaaatca tttatgacct cctaaataaa tctagcgaca ccttttccac ggcacctttt
    15721 accataagca acatttctaa gaattgagtc atattctgtg agatagtctt cccaactaaa
    15781 agaccgacct tcttcagaat ctgccttggc tccttctgaa ttaagccgat tatatctttt
    15841 aatagctaca tctcttgcga taaatgttaa acgtcgtggt atttctaaca gctcttcatc
    15901 agaatactca ttgagtttcg caaggatacg ttcaatactt tcgtcaatgg caagtttaat
    15961 caattcgtct tgtgaggcat cattcttagc aataccttta aaaattttta cctcaactaa
    16021 aagtagttcc ttatccattt attttaccct cctggaactg ctcctgggac ctctttatct
    16081 gcttcaatag ttgcctctac aactccttca ggaatttcag caaatagtac attcgctcca
    16141 aaaaatactg actcataagt taagttagat aattgacgtt gacgagctgc agcaatcaag
    16201 ccagtctcat ctgtgtagtc tgcaaataga ccacctaaat ctccaccacg tacattcaac
    16261 gaagcaaaaa caagattatc aactgcagtt gagtaaactt ttccttctgg cacagatggc
    16321 ataacgataa cattctgcat ccctaaaaag ttctttagta acgtcatccc aaaaacatta
    16381 gaagcatctg cacctacttt agtatcacca aggtaatttg ctacatctaa aggagataca
    16441 aaagaaacaa gttgagaccc ctcaaactca ttaaaagtag ctagttttgc ccatgataaa
    16501 gatagtgcct tctgaagacc agccccttta acttttgttg gttttgtttt gaggaatgta
    16561 aagaacgcat ccttaattcc attctgaagc tcacgcataa tgcgcttatc tgcttcagtg
    16621 atagcacgag ctgcaccatg gcgagcaatt gcctcagctg tagttgcacg acgtttcttg
    16681 aaccacttca ctgtatagtc tttatcttta gttcgagtaa ccttagacag agggattgtt
    16741 tccccttctc caggatcagt ttgatctaaa gtcacttccc acttataggt ctgaattttt
    16801 aaatcactgg ttaactcttc acgacgagta acccctagta gtttcagtaa gtcattaatg
    16861 tttttggaaa acttgttaac aaaatcaatc gatttaatgt ctcccaagtc tgccatggtg
    16921 tttaaatttt tttcagccat aatattcctt tcttattctt aaaataagtt acgattttca
    16981 gcaattaaac gcatacgttc gtcgtcgtct tctactgcca taatctcagc tttagtcatg
    17041 ccgacagagc cgttgccagt ccgtggtggc ttctgagtca aacgattatt aacgcgcttt
    17101 tcaacagcct tatcaaaaat aatttgtaaa ttatcaatat tagtcttaac ttcttctgca
    17161 gtagtaccta aaacaacatc aataaattct aatggcaaac ctttttccac aaggagcgtt
    17221 tgagtctcaa tacgcatttc acgttctgcg actgcttttt ctcgttctgc gattgcatca
    17281 agacgttttt gttcttcttc cttagcacgt tcatccttgg acattttggc caatcgttcg
    17341 ccctcagtac gtgccttttc aatagcgtca gattgttctg cttcccattt agctcgctca
    17401 gcagtcatca tttttgaaat atccgcacga gtaaatgtac gctcatgttc ttgcttagct
    17461 tgttcaacag cttctttctg agtgtcgact tgctcagtag tttctgttgc tacattttct
    17521 tctgccataa ttttctcctt tagtggttac gccaccaacc gatagtctag ttttacgtcc
    17581 tgcgacgaaa cagtgtagct tttagtgtca tcaacaagtt tggacataat aaaaagccgt
    17641 atcgctacga ctaatctaaa tgattagtaa ttatttcttc cattttttct tatagttatt
    17701 ctttatataa tcaagttcgt tatttgtcgc ttgagcatta tgttctacaa tttgttcaag
    17761 ttgttcaatt cttttatgtt gatttctaag agcttctgct tgcatggcat tctctgcgat
    17821 aagcatcaca atagctcgtt cgagtttacg tttcttctta attcgtttat tcattgcttt
    17881 atccttcctt cttcacttct gcaataattg acttaactgc tattgctgca atgccaataa
    17941 tcaatatgag caccaataca cccagtgcta ctaataccat tcgccaaata aacatatctt
    18001 cctccttgat aattctgcta tactggtgta tacttatggt agtgaaagac ggtggtctaa
    18061 cgcccatgaa gcagttttct gtggaggcgg tgggtcatcg tctttctttt tctactacag
    18121 ctaacacctc gtctttgttt ttgataattg atatattgag acccctgcga tctatcttat
    18181 aaatatgttc taactgcccc agtatttctt caaaagatag tggggttttt gtgatatcaa
    18241 aaatgatatt aggtgcttgc ttttttgctt ttcgtagatt accatcgatg acgtttttac
    18301 ctgaacctgt gatttctttc aagtcaaatg gaacgtcgtc aactaaatag tctggagtgt
    18361 taatattttc ggggaaatta accctcggaa ccatttcaac ttgtaatcct gtcttcttag
    18421 ataaccagtt agcaactcta tattcgtagt ttgaatggtc aagtactacg ttatgaccat
    18481 caactctgta agttccgcct cctttagtgt actcattcag catagatatt tgagactcat
    18541 actcttgccc atctatccaa tctgatgtga catccttaaa atacttagta cttttatcag
    18601 cgataggttc aacatcggta ttaagttcat cctcgtcagg aataattgta gtcctacaat
    18661 taaagtgata tggagcagca ttaattccgg gctgtatttc agataagagt ttctttttat
    18721 tttctcttgc cttacttcga catatctgag tagttctatc atctagctga actaagtctc
    18781 gataatactt aatacctgca tcaatgtatc gcttggctgt agcattattg ataatcatcg
    18841 tgccatcagt acgaataagc gtttcagccc tatgtctggc tacgtcgtaa cgtttgccca
    18901 attcacgagc catttctcta ggaccaagac ctctgacaaa tccttttttg aatgcttctt
    18961 gtaaatcttt tactagatta tcaacatttc cccagagttg ctctgagtaa ttatagccgt
    19021 caaatggtgt cttgattaac tgttctagag ctggcccatt aattgttcct gagcgaccac
    19081 ctacagattt ttggtaagca taactagctg cctttcttag atgatcgcta aacaaaccat
    19141 ctaagatacc tctcatagta cctgcacgat gtataagctc taaattaagc atgtcaagac
    19201 gagtagcctg cgctgataca tattgttcat taatacgttt aagtagctct gggtctttct
    19261 cagcttgctc tctatatcga ttggcatttg ctgcataatc acttaaatct tcgtgtctaa
    19321 gcttcttaac agcatcttga taagttatct tattatcttc agcaaaacga ttataaaaat
    19381 catataaatc cttttgtaaa gacaatgatt ggttactata tactctagat aattcattga
    19441 aaatatctaa gtctgctcta tctaaataac taacaatatc ttcttgacgt ttttgccagt
    19501 aatcaagatg gtcctgctgt tggttcttct tgtccattag catcacctac atttcttggt
    19561 tctggcatta cttgcttctc gtcagcctct tctttcagac gttttaattc agcttcagca
    19621 tcgacaccag ttactgcgtt taaaatttca aaaatagttt gatcgctaac aattccataa
    19681 agactctgtg cagccgaaac aatttcatta tcattctgcg gcacattagg agtaaatact
    19741 atactagtat cattaacttg actatatacc gtcgattcgt tccccttgat tgaccagata
    19801 ttggcagcta aacgtaaacg tctcatgagt ccttttttga acaaccgttc ttgcttttca
    19861 cggtaattat ctgaagccat tagcttatac ttcattgact cgccagattg aacaccagag
    19921 aattttaaat cttgaatatc gggagtaaaa gtaaagcgta agatatctga cactagtctg
    19981 tttttatacg cttcacttcc tgccatatca tattcttttt ttagataata cgcatctggc
    20041 ttagcaccat ccgggttagg attgtcatca aggacaataa cttttgcctt cttgaatcca
    20101 atagatatac ctaaacgacc gtttggattt atacgtccat cttcaaaaaa atcattttcg
    20161 tctgctcctg tatacggatt accagagaga actaggattg catctacaga atcttgttga
    20221 aagttagcaa gctctgactg tgataagtcg tatgcatcaa tattatctaa gactgattca
    20281 taagcgcctg ctctatcatc attattagaa tattcattaa ctggaacacc actaaagtaa
    20341 tgttcttctt catctttcag actcattcct tttgtttctt gattaaagtc ctcgtaagta
    20401 aatatcatat ttgaagtata gaccttaaca atttgcttac gtttacctgc gccgtaatcg
    20461 atgtcgtaga aatgtacagc catcaaagaa ttatgttgat aagtatcatc ataaataaca
    20521 aatgtctgtt ctgcgggtag ttgatagagt ttaatttctg tcccattatc tacttcttca
    20581 acggttaaaa gctcgtaagc acgaccgtaa atggataagt ccgttttgat tgctacattg
    20641 tgatagtctt cgttgttacg aacactcata agattaatgt ttttttgaat atcactatct
    20701 tcgttcttgt actcgacagg cacaccaagc atataacctt gctcaaagac tgtaatgtac
    20761 ttagcaaaat cactagcaat acgattgtca gctgcatatt catcggtctt tgctggacga
    20821 tatttgatat tgttatcacc aagataatat cgctttaact ctttcaatcg gtctagttgc
    20881 tcagtctcaa aactagaaat atattttttt aactgatcaa tccatttctg cgaattatat
    20941 tcaatagcat caaaatcttc ttgcaacatg ataaattgcc tgttcgcatt ttcattaaat
    21001 cttgttcctg ataaaaattt tctttcaaac aagttcaaat ccctctctat ctaaaataat
    21061 atttagcatt atttagtcgt ttatttctgt cagatgattg atatattcta tcttgtactg
    21121 catatctgat agcatcaatg caatggttat agctgtcaac tggctcattg atatactcgt
    21181 tagttttctt atcttttttc caagtgtaat tctccagctc ttcaattgtt ttaacacacc
    21241 tttcgtcaac aacgatatcg tactgtagca tgtactgaat tccttgcata actgaaccag
    21301 ctcctttttt tacatcaatc attctttgaa tacctaaatt tctcaactct tggttagatt
    21361 tcttttcagc tgaatcccct ctgatttctt ctttggcata tccgaggctc tttatagcat
    21421 tagctatttt atcattggtc aaattctttc tgacatattc ctccaagatg tatagcttct
    21481 tgtttgcatc atcgattttg acatgcaaaa atgccgaagg atcattaatg aatccgtagt
    21541 ccaaaccaaa aaaagaaggc aagtgtgata acttgtcttt gtttagtatt cttttttcat
    21601 actttggaaa tatgagcttt tctaaagtcg cgaactgacc tagagcataa atcttgtaat
    21661 aagcttcgtt tctgtttgct agttcctcta tattttctct agtaacatca tctaaaaacc
    21721 tattatcttt atatgtagtt tgataaacta cagtgttttt aggtgacttg ataaagaaag
    21781 ctttataaac ccagtttgct ttactcacag gattgaacat gagatagact tgcttctcta
    21841 gatgtttctt atcccttaaa cgcaaagtca actgtgtgta atcatcaagt gtaaactcac
    21901 tagcttcttc cattacaaca tcagatatcc ctttaattga cttaatcttt tctggattat
    21961 ccatgccttt gaaaataaat tccgagccat tcggcaatgt tattctaaaa gctgacatat
    22021 ttatcttgca tttatccaag acgccaaaat acgacaagtt agacataata tcagcaaata
    22081 cagagtctct gacagttgca ccaacttttc gaaggacaag aatctttctc ggatgtttaa
    22141 actttggatt gagtgctttt aagataattt tttggaatac accgtgtgat tttccagaag
    22201 atgcacctcc atagtgtacc tctgtgaagt tatcgtaatt atatagttta tcgtagatat
    22261 gtttattaaa gactttgcta ggatacttga tgacaatatt aattttaggg cgttgcttag
    22321 tcgtcatccc actcacctac actgatggtt acatctgaac ttatatccat ttcaattttg
    22381 tcagtgaata atctgtaccg cttacctagt agctcagctg ccttgattct atcttttgca
    22441 cctacatcaa tatcgacaat ctcttgtcct aactccccga tgctgatcag tgttttttct
    22501 tgctgatcgc ctcgcatgat tgatgtaagg tattgtaaaa cttcttcttg cgtggcaatc
    22561 tttgcagatt caagcttttc aatcctttca tctatataag ctttaatgtc aggtttggtc
    22621 aagttttctt gacctattga cctcgctgtc tttttactgt aacctgcttt aatagccgct
    22681 gctgtcgcgt tagctgagat gatgtactca tctgcaaaac gcttttgttt tagtgttaat
    22741 ttactcaatt ttccatcacc tccttcgtga cataataaaa agacaagaca ctatttgcct
    22801 tgccttcaaa ctcatactac caatttatca tcaaaaaagt gagaaaacca cccttttttg
    22861 tctcaaactt acaattttcc aatttgttgt gcaaatagag tcaaaattct atcccttttt
    22921 cggtaggttc ctgctctaga cgtatgaatc ttataagcta tctcctccca ggtattatta
    22981 gaaccagttc cccaccttag atcaaaaata taattcaatt catcgtcaag cagtgcttta
    23041 accctatcaa cactttctct gaagttttca taacttttga ttttaccgtc ctttgaccac
    23101 tttatgacaa tatcttctgt ctctttagct actagattag acttcccccc gcctacattg
    23161 atatctgtac taacaggcgt ctgcagctca agttttctaa ttgcaatttt acgttcaata
    23221 tcgcgataat caaacaacca ctcatcaaaa gcctttaact gtgcgttaga taatttactc
    23281 attccgtcac ctcttctcta aactgccgat aataaacggt aaaaataata ttaaaagtat
    23341 caaaacaact gctgtgttat catccattct tatctcctgt tctccaaaaa ataaataaat
    23401 ctctacttag tacaactggt ttaccaaaga ttttatatga tgactcaaga ttttttattt
    23461 caacatcaaa accatcacca agtcggtatt ttagtaaatc tattgttttt tggctgtcaa
    23521 gtcgtctagc taagtattca tcattttttg gaatagagat tttataacca gagaatcctt
    23581 tcatcgcaga ctgtttaagt ttctcctcga tttttaaacc atcaaagtac ctatcaaacc
    23641 attttttgtg agattctgaa ccgcattctt ttacttcatc aattaatgac aactttatcc
    23701 cccatttcct gtcaactccg caatccgctt tgtctgtctc tgattttgct ctgacgactc
    23761 tttcaaacgg tactgcgtgc gtattagttg ctgttgtaaa cctgtgattt gtggttggta
    23821 gtagttgtac aatgcaatat ttgttgttaa caacacgctg actgctacag ttaatacaac
    23881 aatcacaatt gactgtttgt gtaatttgtt gttttgttcg ttcattcgtt cacactttct
    23941 agtaaccaat attcaacgag acgttttgta tattcgagtg ctttttctaa atcctgagtc
    24001 gcattgcctt tgtatctatg tcgtttaatg tatttatcaa tgtgactttc catgattgcg
    24061 acaaactggt cccatgggta tgtcttatac cacgactcaa tcaaatcaat ttccccatga
    24121 ttataatgct ttggtttatg tactaattca cctgtcattt tgttttcctt tctagccact
    24181 cccaaatcat gtggaattgg ccattcacca aatcgtcgtt gccataactc tcgcaaatat
    24241 caacgattga catattaacc catacccaat attgttcagt tccaaaacca agctcctgca
    24301 tttttttgtt actttctctc atccagccag gaaccactcg ttcaaaaaaa tcaatataat
    24361 caattttcat aactcttcca ccttcacata tattcctact gtatcagccc agaacttctc
    24421 aataatttcc gaagcaacct gcgcatcatc attccaaaat cccaattcag tcatgcaatc
    24481 tttaggtaac ttgttcaaat tatctgtgtc gggtttagta tctttatatt gtccgtttac
    24541 tgattttttg attttcggaa ataaccactt ggttgttagt cttattggac catttaattt
    24601 ttctttaggg acatgttgag caaacttatc cataaacatt gccctagctt cctttagtga
    24661 atctggttca tagaattgtg gtttcccatt taccacacgg accttttttt gctgatgtgt
    24721 tgtggttggt atttttttca ttgggataaa aaattcaatt gtcatgatca acattatcca
    24781 agtttatttt ttcaaattgc tgcacgttgt tctaaaattt ttagataagc tcccattgca
    24841 tgccattgtg ctactaataa ttcaatagga cattgtaatt taaaatctag actatcaata
    24901 taaaatttat taattaatac tgataatttt tctgcacggc tttttaattc ttgatattta
    24961 acaatcattc tttttttgta ttctttcatt tttgtcactt cccatctttt tttattttcg
    25021 cgcatagtca atgtcaaggg acatggttac agggttacaa ggggcggatg catagccccc
    25081 ttgttcctgt tcatgtaccc attgaccaat tgggacattt cctaaatatt cttctttcgg
    25141 agaaagagaa tattctgtcc ctcaattttg tccctcaaaa gtcaaggaca ttttcgagaa
    25201 attttcgagt ttgtccctat ttttgtccct gaaaatcatg taatagggac acggacattt
    25261 tcgagtttgt ccctattttt cagggacatt ttcgagggac actttcgaat ttgaccctcg
    25321 attttgtctg tccctgtccc tgtccctctg tgaaatattc acaaactttt attggtttta
    25381 ggttcttcta ttggtaaaat ttgtttattt ttaacttcaa atttaccgtt ttcttttatc
    25441 catctacgaa tagttttttc agaaactggc ttatcttcag tagaaaaata ttcgattaag
    25501 ttatcgattg tgactggctc tattccatca ttcaatgaat taattgcagt ctctactttt
    25561 tgagatttct tttccctgga ctctcttgga ctcttcttct caaaattctt cttccaagtc
    25621 ggcacattat cttctagtga tatatcagcc aagacacccg ttgtgtctac atcatgcact
    25681 ggatagctaa accacatgtt aactggttgg aatttagcaa actcgcgtaa agtcccttca
    25741 actcgccagg cagtggaaat ctccacctca tactgggctt tcttgaccgc ttccagatag
    25801 ggctgtttaa tcatgatgtc cttgatagcc ttgtcaaaat gttgctgcat ctgataacga
    25861 ctttccaaat catctagcgt tacttcctgt tgatagtagg ctagcgcttt ttcttgcaag
    25921 gcttgttggt aaatcttagc ggctgctttt tctgagcgtg acttgatgag ttcctcagtc
    25981 aattctagct ctactaggtc aatcagtgca tcagggtcac gggcaaacac tcctgaacca
    26041 cttgcacggt ccatagattt tttaccgcct tggctcccct ttgagtgatg gtggcagtaa
    26101 atcacactac aacctaactc agtagctact ttgtcaaact gattggtaaa gtgagccatt
    26161 tggtccgcac tattttcatc accagtcagc accttataga tagggtcaat gatgaccgct
    26221 tggtaatttt tctttaagct acgacggata agcttaggcg ctaacttgtc catgggaacg
    26281 gtctttccac gtaggttcca aatatcaata ttcttgatgt ttgctggtgg caagctcata
    26341 gcttcgtata catcacggaa gcgatgcaaa gctgacggtc tatccagttc cagattgaca
    26401 tacaagacct taccttgttc acactgccag cctaaccatt ctttaccttc agccagcgcg
    26461 atggatagct caattaatgc aaacgactta ccagcttttg atggtccagc aatcaacatc
    26521 ttgtggcctt gacgcaaaac tcctttgatg agttccggtg ccaattctgg catattatcc
    26581 cattcgtcag ctagcgtctc tgggtcaggc aaatcgtcat ttaaatcttc aatccattga
    26641 taccattctt cgtagttggt tttacccaca ttagtatcaa tcaagaattg cttgtgccca
    26701 ttccgagtca caccaggcat gcgtgataat cgactagggt tgcggttctg tgtgtcaata
    26761 tcaagtccat tctttttaca aatttgatag atataatcaa cccgtttgcg gtactcctga
    26821 taatcacgag catctacttt gacaatagcg tgtaatgatt ttttaccgct atggactaag
    26881 gttgctattg gcaattctag ttctttaaac aaagcatact gtttaccaag ttccatgctg
    26941 tcggattcaa ccaaggcata tctaaagtct gttacattgt cattcttgac accatttcca
    27001 tctagtggat taaaacgaat ccatgcacca gcctcttctt tgaagtctcc aaatactgct
    27061 cctaagtcag taggattttt ttggagtgat tcaatcagtt gaccagcagt cctgtcatac
    27121 gctccttttg ttggtttata aacaatctct cccgtatcaa gggtaattgg ataggtctct
    27181 gttacataac caactttgtc agttgattca aataaggtat ctatgtaagt aattaaatct
    27241 tgaactggat tccagtttct tggctcttga atctctttcg attcaatcca atttttatcg
    27301 acaatcttat aatcacgatc gattgtatcg tcccagctga gttcatgagc atcatcgctg
    27361 ttacgatact ctgatgatgt ccatccatta tctttagcta gctgtgtgat tgttgcccct
    27421 gtgatagtcc caaagccatt gccttggaaa ctatcccatt ttgcaaaaca ctcacctttt
    27481 ttatatctac tatcagattg tgaccaaaca tcccaatcca ttgctgtata accttcatgc
    27541 tttagggcca ttcctacttg aacccatgtt tgatagtcta ccgtggcagg attgatataa
    27601 tccagcaacg gtagcaaatt aaaatcattc tctgccatgt tatcctttct gcacatgtta
    27661 tcctttctgc atttgatata attaacctaa agaaaggagg tcaactatat gcctgaaata
    27721 aaaattactt tttcagatgg ttctattgtt atatttcacg aagaacagag ttttcaaagt
    27781 gtagtaaaat caaatgatgc tatgtcctta tcaaaaatat attcattatg gaaccatgtt
    27841 catgatggat taattcctag cttcttagaa ttaattgcta actcgcaatt tttctttgac
    27901 ttagaaaacc cacaaattta ttacgcttca aatgctgtta taaaaattga agctatttaa
    27961 taattctgct attcaaggca atatctctga ttttttcttg aatagctttt tttactactt
    28021 gcccaaactc ggtgagactt gagtgtttat cgacatataa aattgcctcc gaatatgttt
    28081 cggatttttc gattgcttca tcagcaaaat ttgaaataaa cttttcaatc tgttcattaa
    28141 tattttccat tttttctcct attctggttg atattctcga gcattgatgc cttttggtag
    28201 gcgccatccg ctagcagcaa tacgattaat catgtttgat gctacttcaa atttccacat
    28261 acctacattt ttgaaaccgt atcgctcaag cattcgtatt tgttttggag ttgttagacc
    28321 tgattgacga cgtttatcca gtctatctaa taatttggca gccttaccag cattcccaat
    28381 ttcgtcagta aatatgccaa atttctcaag tgttttaagt tgtttttcag aagctggcgc
    28441 catctcccaa ccaaattccg gtacatagtc cactaagtct tcagcgttga tagacatttc
    28501 aaattgcaac ggatcaacta atgcacgttt acgtttacgc atttctgcta actgcttagc
    28561 gagtgcttct tctcgctctg caacaacatc tttgctggct acttcttcag cttctaacaa
    28621 ttcaaaagct acttcagttt catcggtcat gttctcgacc attttttgtg caacttctgg
    28681 tgtttttgca attagatgag caggacgaca aagctcatgg cgttcggtat gccataaaaa
    28741 atctaataac agtagttttt ctttccccgg cgctaatcgt gtgccacgac cgaccatttg
    28801 actataaaga gctcttactt ttgtgggtct cagtacaact acgcaatcga ctgtcgggca
    28861 atcccatcct tcagttaaaa gcattgaatt gcatagtaca ttatatttat cattatcaaa
    28921 gtcttctagg acttcagcac ggtctttcga ttctccattg acctctgctg ctttgaatcc
    28981 tttttggttt agaatatcac ggaatttttg ggaagtttta acaagtggta aaaatacaac
    29041 tgttttgcga ttagaacatt gcttaaccat ttcatcggca atttgttcta ggtacgggtc
    29101 aagcgccgtc ccaatctcac ttgctttaaa atctccagct tgctgactca ctgtagatag
    29161 gtccaaagtc aaaggaatag taactgctgt aatttttgat aaataaccag atttgatagc
    29221 atctaccaaa gaatactcat aagctaaact atcgaaatat ttacctagat tttgtttgtc
    29281 accacgatct ggtgttgctg taactcccaa aacattgcta ctatcaaagt gttgtagaac
    29341 tctttggtaa ccgtctgaaa tagcatgatg cgcttcatca acaacaattg tgtcgaagta
    29401 atctgttgga aatttactca aacgcttttc tcgctgcaac gtctgcacag aaccaacaac
    29461 cactcgatac catgagccga gcgatgtact ttctgctttt tctaaagccg tgcccaatcc
    29521 tgttgctgtc attagcttat cacttgcttg ttccagcaac tctgaccgat gagccaatac
    29581 taaaacacgt tcgcctagtt taactctatc ttcaataatc tttgagaaga caatagtctt
    29641 accacaacca gtaggaagga caagaagggt tttcttcttg ccttgttccc actcttgttg
    29701 cactgctgac cttgcctctt cttgatattc tctaagtttg atgtaaatca cactcctttt
    29761 tgtgtaggtg tcgttaaagc ttttgtaata ctccaaccac gatttaatct tgtaagtaaa
    29821 gtaatattag aaataccaag ttcacttgac cattgtttta ttgtctgagt cttaccttta
    29881 aatgtgatta aatggttatt tctttgatta tttgcttgtt cggttgaatt agcccatcta
    29941 caattagatg gttcataatt tccgttaaca tcaatacggt caatagataa attgtctcga
    30001 taaccattcg atgatgccca ttgtttgaat tgaatatatt cttcccattc gtcacatata
    30061 gttattccac ggccaccgta tttaatataa tcattggaat ttttatctct gcagcgttgt
    30121 ctcattccaa cccaaatatt ataaagacgc tcttttctag caaaaccatg tttagtaaca
    30181 atgcatccgc aactggtact atgaccagtt gctagattac tactattaac aatcgttaac
    30241 ttaccgcaat cacattgaca tttatatttt gcttttgtat atggatcctg aggtaaacgc
    30301 tcaatcacta caagcctacc aaatcgttga ccagtgtaat ctttagcttt tcccattaga
    30361 atcctcccca tccaccttgt tgttgagatg cttgcatttg accttgttga tatgactgag
    30421 gtgctggttg ttgttgaggt gcaaaattag ttgtttgctg agctggttgt tgatacgttg
    30481 gttgtggcgc ttgtggttga gtatttgcat tcaatacttt agtccagtca acgctatcag
    30541 cataaatcat cgatttcact tcattataaa cattgtcgtt atatgttcta ttcttaacac
    30601 tacatacacc cgtcgcacca actactgtgt tccaattcat ttttaatggc tcaccgtgtt
    30661 ttttctgacc aattgcccca aagaatgctg ataacattcc ttcggtagag ctatgtaaaa
    30721 atagattatg cttcaaacta gcaacacctt catcagtttc aatttcaaga gaaattactg
    30781 ctttattaca tgctggcaat ttccctgttt tttgtggatt tggtgtgtgg cgtgcacgtt
    30841 caaagtctgt tactgtgaag acatagtcac caggttgtaa tgtaataaat tcttttgcat
    30901 cttcttggat tgtgtcatcc catccaaatt cacgttcaaa gttgttattg tattcagtca
    30961 ttttatttct cctattttaa ttttaaaaag tattgttagc tattggaata aatccaattt
    31021 ttgttatatt ttgctattat ttctaattcc caaaatctat gagccttcca ctgtaaaggg
    31081 ctgttctgtt gctcggactt gtgttttaat aacttccaat gtggctcccc aatttgctac
    31141 aatcatgttc cagtaatctg gtggaaagtt ttcaatagga gtcaatggag ggaagtgtcc
    31201 tctaatattt gctactgcta ctaactcatc tggtgtaact tgttcaggcg tcatcaaatc
    31261 cgtcaaagcc tttggtagga cagatggata ctctgttgct gcagttggtc ccgacatttg
    31321 cgcctgttgc tcatgtacag gttcgtgtat aggttgagta ggcaccggtt gctgtactgg
    31381 cggtgtttct gctactggtt gacttggtgc ttgtgcgaat atatgtgcga taccagcgta
    31441 atcaagtggc atttcttctt gtaatccatg acggtttttt gcatcccacg ctggatgatg
    31501 ttgtgtatat aagacacggg taccgccagt tgctttcttc ttgttgttat cagctgtcat
    31561 cactacagtt ttataattag cgaacagaac catgtcagcc cattctttaa ctaatggtgc
    31621 agtctgtgag cttgtctttt tacctagctt gagttcccag cgatcataag ctcccatttc
    31681 atcaggttgc tcaaatttac gcatttgggc atgagcagta aggacgacat tgatacctaa
    31741 ctctatcaat tcctgcaaca tatttaggaa acgtccaact tcttcttttg tgtaagtgta
    31801 accattaccc caaccaaaat cttcaattcc tttttttcca tgttgagcac agacatcatc
    31861 tactattaag ctttctgccc agtcaatagt gtcaatgaca agtgtcttac agcatgtagg
    31921 gttagctttt acaaaagcaa tttgattttt tagcatagtg tagcttgtag gcttgtccat
    31981 gcgtgccacg tccatgttgt cagttgaacc ctctgtgtct ataaacaacg gctctggaaa
    32041 ttgcgctgca aaacttgact tcccaattcc ttctggtcca tagataatga cacgttgtgc
    32101 ccttgcccgt tttcctctag taatttgcat atttacctct ctttctaaaa tccaccttgc
    32161 caagtcggtg ctactgtttc agcaaaacta ccaccctctg ctgttttaaa gctatgggct
    32221 ttattttcca ctgagtatcc atcttctata atgatggcac actcttcgcc agttgataca
    32281 cgggttgcaa tagcttgcaa accctcctgc tctagccatt taccaaattg ttcaagtgtg
    32341 atgttgtcca tctgttcgag tttatcaatc aaaacaaagc cacaatctgg ttttagttta
    32401 cgtacaatag cagttgctac tattagttgt tggctgcctg acatgttatc ccactcttga
    32461 ccaaggtaaa gtaactttcc atcatctact gacaagcctt caagtggcag atctgcatta
    32521 atcaataagt ctcgtttttg ttgacgaatt gactcaatat cattagtcaa tatgttatat
    32581 tgttcacgct gatgtttagc atcttcttca gctttatctt tatccagatt agctctgact
    32641 ttgcggttga cttcttcaat attagaaatg ttttcttcta tttctgcagt tgattcatct
    32701 attaatgaca ttgtgtctct ttgtgcaatt tccaagtcac tagataactc ttgtaattgt
    32761 ttctctgctg tggctaattg ttgttttaaa tgttcaacct ctgactgttt aaattgatga
    32821 ttttgttgaa taacggatag attctgacgt tttctagcgt tttccccatt ttttgctaac
    32881 acttcttgtt gttgctggat taaatcagcg atactaatta attcttttgg ggcatcatta
    32941 tagtatggtt gctccttagc aaacttttct ttttggtcag caataacacc aatagtatga
    33001 cgttgattat agagttcttt ttctttgagt tctaattctg ccaactgatc accaactcca
    33061 atgatttcaa gtaagacatc tgctttttct tttggagttc catccataaa ttttggtaag
    33121 ttaatagcta actcttctac aaagctatca agcaattgtt gaccaccttt ttgcccgtta
    33181 ggatcaataa ctttcaaact agaattctta cccttacgct caacaatcaa accatttgac
    33241 atggtaattt taagcgtagg tggtaccata gttccctctc gtgctgcttg actaggttta
    33301 aacttattac cacctaatac ccaagcaata ctatccagaa cgctagtttt accctgattg
    33361 ttattgccac cgatgatggt taatcctttg gatgatggtt caacttttac agctttaatg
    33421 cgctttacgt tttcgatttc taacttatta attgtgattg tcattgtcaa aatctcctat
    33481 tactaattta ctttcggtaa ctactttttc agaactaata atttgagcgt tagcaagacc
    33541 atagtttagt agtgcatcag ttacttcttt tagtgttaaa tcacattcat ttgcaatatt
    33601 ggcgatttca tcataaatat tatttgaaca tctaattcgt gagtacgtat tttttggttg
    33661 tctttcaact tcaatcatta tcatcactcc aatttttttc tagtgtttgt ctatcaacgt
    33721 tatctaactt agcaatacat ttttccagtg tgctatctaa gatgtaacct tcgttgacga
    33781 taatatccaa taaatttgct tttgctacgt tcgtaaaata ataatctgcc atttcattag
    33841 ttagtcgtct attttcatca cgcaacagtt cattttctag taatacttct tttaacatgt
    33901 acgccctcta ttattacgtt caatcatatt gtcgtaacgt cgtgcattag cttcccagcc
    33961 atgtgtttcg attgtccatt tttgacgtgt ttctataacc tgatttttct ttacaaataa
    34021 ttttttaaat aatttcttca ttatgttata ctcctaactg tctttccttt ttaatatttt
    34081 ccaacatttc tgccagcgtt tcctttttag gtttatatct atttctagac ttccaagtaa
    34141 caaacaactt aaatccctcg taattgatga atacaattct atgcgttgga ttattgataa
    34201 attgtttaaa atctggatga tttctcattt ctgctgccca aacctttgcc gttccgacgg
    34261 ttaatccctc ccagatttgg caaagatgca cataatcgcc atagtcggct ttttccgact
    34321 gcttagctgg tctgtagact aattcagctt ttggcatttc ttacctccaa tctgtgatat
    34381 aatgtaagta gaatttttga taaggggctg actgcaatca gttccttttt ttgcatttac
    34441 tcaatgctgt aatctgcaat cactcgcaga ataaactggt tagcctttgg tcctctagtt
    34501 gagccactaa gaatgttagt gacctcttga cgattgagac cataggctgc tgctaaatca
    34561 ctcttcttga tattgttact ttttagaaag gtaacaactt tttctcgtcc aactgcaata
    34621 tctggcatat tttctccttt cttttctgat ataataaaaa taaaaacttg aggtaaaaat
    34681 gtctcgatac tctgattata aatacgctaa tactatcctt aaaagtattg aagaacgacc
    34741 agaaaaatat ctaattatcc attactcctg cgaaagtttt tataatctag gtagtaaaag
    34801 tcctagaata gcttccattt cagttcgtca attcaataat gcccaaacca acaacttttc
    34861 cattcatcag tattccgaga tattagatac cccaattact gatgatactt atgaaactat
    34921 cgaaaaagag ctgttaacag atttcttcac atttgttgac agaaataccg acaaaaattg
    34981 gattcactgg aacatgagag atactgtctt tggctttcat gctattgagc agcgattcaa
    35041 agttctaggc ggcacaccaa taatcattga tgatgacaaa aagatcgatt tagcctatct
    35101 gtttaaaaag atgtacggtg gaggatatat tgacaatccg catattgaaa aattacttga
    35161 attgaacaat ctaaccccac atcgttttct gacaggtaaa caagaagctg acgctttctc
    35221 ttcgagacaa taccatgaac taagtatgtc aacatcaagc aaggtcaata gcttttcaac
    35281 attcctcact ttagctatca acgatgattt aaaaactaac acccctaaat ggaaaatgag
    35341 aggaactaac ctatctggat tactttccgc ttttcaagaa acaacctcag gaaaagtgat
    35401 aatcggtata atcaacctta ttgtcggcgg aatagttggt gcaataattg caaaatatat
    35461 tcaataaatg atttcttttg aagtagaata agtttttgtt gtacttgctc tacttctttt
    35521 tttgcatcct ctttggaaat aagagtcgga ttgtcgtacc aatctctttc cacgtaaagt
    35581 tcgttttctt tcccctctaa gctatcagcg acccactgaa aatgctcatt acgcttttgt
    35641 agatcaacga tgtttttttt cattttccct ccttgtttta aaatctgtaa acaagaaaca
    35701 actaaatttt caacttttta gtatataacc acttgacaac tcacacctaa aaagttagaa
    35761 ttaaagcata ttaaagaacc ttaacaaatc aactaaaacc cgttcgccaa aactgtttta
    35821 tagttgtttg cttagttgtt tttttagtta tctcttgctt acaaaaaata tgatacacct
    35881 atttggttga attgtcaaca tttttcaccc aaaaagttga aatatttttt gtcatgcctt
    35941 agaaaggttg ataaatcaat gtttccgacg tttgaaaaaa tcaaagaatt gtgcaaacag
    36001 cgtggaatta cacttattca actagaggaa accttaggat atagccgaaa tacactctat
    36061 aagttgaaaa ctcaaaaacc caacgctgaa agaattgctg aaatagcaga ctacttcaat
    36121 gtctctacag attacttatt aggtagaaca gataatccca atatagctaa agatagccaa
    36181 gaatacactt ctgacgatct tagaaaaatg gcagaaaagg ctaaaacatt tgatggcaag
    36241 ccacttactg aatcagatat tgatgcaata caaaatatta ttgaaattta tttgaaagga
    36301 agatagtcta tgactattga agagattgta gactgtcaca aagttaatct cgcatacttt
    36361 gataacgaat tatggccacg acctgggatt tatatagacg atattaaagt tatttttgtc
    36421 aataaatcac tatcgttaga tgctcaaaaa cgggttatcc tgcatgaact cggacattgt
    36481 gaaagaaata ttggtgacta taagacacta cgtgagaaac acgagctaga agctgacaga
    36541 tacatgatta gtcatttaat cgctgaagca ctttctcaat tagaagataa acgagagttt
    36601 aactacctac aatttatgca atatcatcaa ctaacaacta ctgcagatga atgcatggta
    36661 attgatgaat actataattt acttaaggtc gtgtaatata gtgaggagat tatatggata
    36721 taaataaaat aaaggaattt gctgtgtcaa catcagaaaa ggctaaagac ggcattatta
    36781 aagcaaacga aatgagaaaa agggcatcac aagaaagcaa aatagtctta ccgcccacaa
    36841 ttaatttagg ttttaataca cctactgcta ttcgtaaaac agtggagggt gactactata
    36901 ttggttttta ttctgaaaaa cctgatttat ttaaatttgt ggattttcag tttgatggtt
    36961 cacgaattat tgaaaaaaca gtaacaaaag gaaaaacgac tcaaaaaggt cgttctggta
    37021 gtacaattat tggaggatta ataggtgcaa ctatcaatcc tactgctgca gtacttggcg
    37081 gattagctgg tgcaaatcgt ggtaaaaaag gaactattaa cagtacctct actactataa
    37141 ccgaggaaat tccgggtaaa gctcaattaa cactcagaaa tttaaaaaca aatgatataa
    37201 aaataattaa aacaaaacta actcaggctc aagctaacaa tattcaaaat ttttttagca
    37261 aatagaaaaa gccccactct agccggcaag caatgaagtg aggcaaactg tacaataaat
    37321 taggcattaa aaagcccttt ttattgtacc cattttatca agaaatgagg taaaaatcaa
    37381 tgtggtcaga aaaacacaaa agtggaaaag taaattttgt agaaagatat aaagatccat
    37441 atactaataa atggaaacgt acatcagtac tgatggaaaa agatactcct cgaattagaa
    37501 aagaagctca aagaatatta gatgctaaga tagctgatat cgtaagaaag ttgcaaacct
    37561 ctgacatgct atttacaaat ctaattgatg agtggtggat attttaccaa caggaaatta
    37621 aaagatcgtc tattgtcact ttaaaaggca atattcgaga gattagagca gaatttggga
    37681 tcaatatacc tgtagttaat atcgacccta gatatgtcca aaattatctg gataatctcg
    37741 attgctctag aaacaaaaaa gagcgaaaca agtctatgct taatcttatc tttgattatg
    37801 ctgttagtct agatatcatc aaggataatc ctgcaagacg cgctaaactg ccaaaaatca
    37861 agaaaacact taatgattgg aaaaagattg aagaaaaata tcttgaagaa gaagaaatca
    37921 agcggttgtt aaaagaatta tttagaagac ctagcactca ccgcctggga tttctatcag
    37981 aatttatgag tcttaacgga tgccgtattg gtgaggctat tagcatagag ccggataata
    38041 ttgattttaa aaacaaaact ctacagttgc acggaactta tgatcgcact aatggttaca
    38101 taaatggaga aaagacctct ccaaaaaccc ttgcgtccta tcgtgaaacc attatgagca
    38161 aacgtgaaat ggaaatttta caagagatgg agtttattaa cgaactcgaa aaaaatacaa
    38221 ataaacgata cagagacatg gggtatatat tcacgactag gaacggtgtt cctatccaaa
    38281 taaattcctt taatttagca ttgaaaaaag caaacgagcg gcttgaacaa ccaatcaata
    38341 aaaatatcac tagccacatc ttccgtcata ctttagtcag tcgtcttgct gaaaataacg
    38401 taccactaaa agcaatcatg gatcgtgttg gacatgctga tgcaaaaact actgtacaaa
    38461 tttataccca cattacaaag aaaatgaaat ccaacatagc tgatattatg gaaaactact
    38521 aactttatgc cccaaatatg ccccaaatat aaaaaaagaa ccttgtcgct atcaataaac
    38581 attgatatga caaggttttt ctagacgaat tatttaacag cgtctttaag agctttacca
    38641 gctttaaatg ctggaacttt tgatgctgca atttcgattt ctgcaccagt ttgtgggtta
    38701 cgacctttac gagctgcacg ttcacgaact tcaaagttac caaaaccgat taattgtact
    38761 ttttcacctt cagcaaggta atctgctaca gctgcaaaaa ctgcatctac tgctgctgct
    38821 gaatcttttt tagttaattc tgtagcttct gctacttttg caattaaatc ttgtttgtta
    38881 gccatgttaa caaatcctcc aaataaattc taaacttata caacataagt tttaacagat
    38941 tatatgatat ctaaaaaaag gcttaaggtc aagtatttaa cgtcatttta gggctttttc
    39001 ttaaaaatat cgaaactaaa attatcgttt actaaatcaa ttgcagtcgc tttcaaataa
    39061 ataccttctt tattttttaa atcttgcaaa ttaatattaa taaccgattt tttaggtttt
    39121 atatctacaa agtttggcaa tttataagat gattttatat attgtaacac atctttttct
    39181 ggaagcggaa gagttcctac tgaaaatgag gtcactttaa gttgaacagc gccatttgtt
    39241 aaacgataag gttcaaaata gatgtataaa ggcacttcat aacctaataa ttgataagag
    39301 ccttcaaata gtatagaaga tgaagcagca taaatcttat aattcatctt cttagtttga
    39361 tattgtttta aataaagtgc aattgtctta ttcagttgcg acttattggt cgtaaaagta
    39421 cctactttta ctttatcttg taccccagtc gaaatttttc ctgtattagg ctcacgtact
    39481 tgaattaaac gacttgcaat cactgctgtg aaggaaaggt taattgccaa caataagaga
    39541 aatgcccatt tccaaaaatt taaattacgg ccagtttttt cttgtttcat ttattttctc
    39601 cataacggcg ttagacatga tttgatagcc aatattattg ggatgaaaat ggtctccagt
    39661 aaagagagca tcattagtga tacttgcctg actatttgat gactctgtaa taccctcttt
    39721 accatttatt cccttataaa ggcggtcatt aattgggaca aaataaacat tttctgaagc
    39781 atcaactact tcttttgtag ctttattcca attatcaata acggtttgca ttttagttaa
    39841 ttgtggaaag tttaggtaaa aaggattata aatgcctaaa acataaatag gcaatttagg
    39901 attatcttgt cttgcttttg caagaatttc tttcaaacgt tccttatatg cttctgctgg
    39961 tttctcaaag gaatttagtg ataaatgact gagctcttta cgaataacag ccaagacatc
    40021 attaccacca acagttagcg tcaataaatc agctttctct aaatcttttt cgatttgagg
    40081 atctgtcgtc atacgtttta aaatttgttg actagtattc ccagacacac cataattaac
    40141 agaagtcact tggtaagagt atcgattatg gagtgattct gatagcagtg ggacaaaacc
    40201 accttgagag gttgtatccc ccacaccttc ggtcagagaa tctccaagag caacatagtt
    40261 aagtgggata actttctttg ttaggaagtc tttttttgtt aatttaggat ttgattttgg
    40321 aatgatcaag gaaaaaatcc caaaagataa aagaagactt accagaaaaa aagaaagtcc
    40381 ggtaagtatt tttttcttat tcataacgaa ccataacagc aaatgcatcc tcaccagtat
    40441 gagtttggat gattgagcca gtttctaata cggatatagg agcacctaga acagctagtt
    40501 tttctttaaa gttattagcc atatcagctt taccacagta ggagatgcca atttcagcaa
    40561 tttttcgtgt ttgggcactt tcaacaaaat tatctaacca tttgctgaag gtctttagac
    40621 cgcgaccttt aacaatcggt actaattcat ggtttgttag ctccataatt acttttatgt
    40681 taagcaatga acttaggaga cctgttacac gtccaatacg accacctttt actaaatttt
    40741 caagggtcga tacgccaata aataattctg acttctggcg tacttcttcc acacgagcca
    40801 aaattgtatc taaatcagct ccctctttag ctaatttcgc ggcttctaca acctggaatt
    40861 tttgacactg gtctgtaaaa gtagaatcaa taactgtaac atctgcacca gcaatattag
    40921 ctccctggcg tgatgcttca atagttcctg atagtgtatg cgttaaatga attgcaatga
    40981 tatgttccgc accttcattc attaattttt cataaatttc tgcaaaaaca cctacaggtg
    41041 gttgacttgt ttttggcaat tccttacttc cacgcattag gttcaagaat tccccttgtg
    41101 cttttaagtc attgtctgaa tacaaggtac catctatcat aactgacaag ggaacaactg
    41161 taatgtcaag ctctttaata agttctggtt caatggtaat ggaagaatcc gttacaattt
    41221 tgattttact catatcctat cctattctat actttaatat ttcaataccc ttattataac
    41281 aaaatttaag tgtatcaaca ttaatttctc ctaacaatgt tagcttttta aagacgttac
    41341 caaagctaac taccaagtaa ttctttagct tgagtacgtg cagtatcagt tacattgttt
    41401 cctgccaaca ttttagcgat ttcttctaca cgttcttcat agcttaaaag ccttaccgtt
    41461 gaaactgttg aagagtcact ggaaattttt tcaataaaat attggtaatc cgctattgca
    41521 ataacctgag ctaaatgaga aattgctaaa acttgtccat gactaccaat cttgtgaatt
    41581 ttttgagcaa tagcctgggc cacgcgacca gaaactcctg tatccacctc atcaaataca
    41641 atggaagttt tatcttcctt acgtgaaaaa gcagatttaa tagctaacat caatcgagat
    41701 aactctcctc ctgaagccac ttttaccaat ggcttaaagc cctctccggg attcgttgaa
    41761 atataaaatt caacgatttc gttcccttct ttattaaatt ttccttttgt aaattgaact
    41821 tggaaatcag ctttctccat ataaagctct gttaattctt gtttgatttc attttccaat
    41881 tgtttagcta gcttatgacg ctctaaactt aattgatttg ctgattcaat caaatcgtgt
    41941 tctaaaatct ttaattcttt ttctaaagca tctgaggact cttcactacc tgtcaataga
    42001 ctatattctt tagtaatgtt atcaaaatag tctaaaacat cattcacatc accgccatat
    42061 tttcgcgtta ttgtgttaat aacatctagt ctattttcaa tttcttgtag taaaccagcg
    42121 tcaaaatcta aatcatcgat aacgtcacct aaacgtttag taacttcttc aataacgtag
    42181 taagcttctg aaagattggt ggaaagatct ttatattctc gatcaaattc ttctaaagcc
    42241 ataaggtcat tcattgcaga acgaacattc gataaacttg aaaactcttc gttatctaac
    42301 ataagatatg catttgtcaa agtatctgca atattcttat gattcattaa tttatcacgt
    42361 tgcttgagta gcgtttggtc ttcatctgat ttaagggcta cagactcaat ttctgctatt
    42421 tgaaattcta gcatttcaat acgtgattta ttctcttgtt catttttttg cttatccaat
    42481 acacgtttac gaagctgacg ataagcatca aaaagacttt gataacgctc ttttatgaca
    42541 ttaaattctg tattaccaaa ttcatctaac atcagaatgt gcatgttagg tttcattagt
    42601 tcctcttggt catgctgacc atgaatgtct accaaataat gccccacagc ttttagtgtt
    42661 gacaaattca ccatttgccc attaattcga ctaacgctgc gcccattttg aaagatttct
    42721 cggcgaataa ttaattcatc tgctaattca atgccatttt cttccaataa ttggactaat
    42781 gattgatttt tttcaataga gaaaaatcct tcaatttctg ctttgttagc accatggcga
    42841 atcacttcaa cactagcacg ggctcctaac atcatattca tagcatcaat aatgatagat
    42901 ttccctgcac cagtctcacc agttaaaaca gtcattcctg tttcaaaatt aagtgaaatt
    42961 tcttcgataa tagcgaaatt cttaatagaa atttctaaaa gcataaggtc ctctactaat
    43021 cttttttctc caacattgca atcattgatt gacgaatgtg atcagcatct gcttctgatt
    43081 ttgcaatcaa aaggagacta ttatcgtcag ctgtcaaact aaaaatatgt tcttgacaat
    43141 gtgacattat gaaggtttta attaattgac tgttacctgg tatgacatta atattgataa
    43201 attgctctaa acctagtagc ttatctgata ttgataaaat acttgtttta ataggctttg
    43261 ccacagcagt tgtaaagata gggtcgtttt catttgacaa accgtaaata tagcgacctt
    43321 ttgctgatgg cacttttata atgccaattt cattcatatc acgtgaaata gttgcttgag
    43381 ttagagttac cccataagct tccaatctgc gtaacaactc atgttgtgtt tcaacagcat
    43441 ggttaagaac aatttgctta attaaattta aacgttcact cttcttcatt tttcttaaat
    43501 tccttatgtg ctttttctat aacatcttgt atttggtcaa gcacaagatt ttgtggatct
    43561 tgacactttt gcaaatgcat taaaaactca atatttccat gtccaccttg aacgggcgaa
    43621 aaatcaagat gtttaaccgt atatccataa tctttcgtga aattggtcac tgttgtcaaa
    43681 accttttcat gaaccaactt gtctttgaca ataccatttt taccaatttg ctcacgacct
    43741 gcttcaaatt gtggtttaat taatgccact acttgtccac catccactaa aatttctttt
    43801 agagctggta aaatcaaatt aagagagata aatgagacat ctatcgatgc aaattcaggc
    43861 agtccctcct tgaaatcttc tttttgggca tacctaaaat tatattgttc catagaacga
    43921 acacgatgat cctgacgtaa cttccaaact aattgatttg ttcctacatc tactgcgtaa
    43981 actaaacgcg ctcctgattg tagcataaca tcagtaaaac cacccgtaga ggcgccaata
    44041 tctatagtta gcttatctgc aactgaaatt tcaaaaactt gtaaagcttt ttctaatttc
    44101 aatccacctc tactaacata ttttagtttt tcacctttta gttttaattc agtatcgtct
    44161 gcaacctttt cgcctggttt atcataacgt tctccattga taacgttaat caccagtcct
    44221 gccataacac cacgtttcgc ttgctctcgt gtatcaaaaa gtccctgttt ataggcaaga
    44281 acatctaccc tctctttagc cattcaaccg taatccctct attatatcta ttatttttct
    44341 agcattaaag ttagcttttt tctctagatt ttgaaaaatt gcctgagctt ttttcaaagt
    44401 atcgtcaagt atttcctgtg acttatccaa acccaataaa tttggataag ttgtcttttc
    44461 tgctacaatg tctttatttg gtgtcttacc caattcttca aaactagcag tcacatctaa
    44521 aatatcatca cgtacttgaa aagcatgacc gattaaaagg ccagcttgat gcagttttcc
    44581 ttttacttct tccgacttct cagctaaaat acctgctgca acaaagggat aagctaacag
    44641 gcgtcccgtt ttattaatat gaatcaagct taaatcgtca atagataaaa ctttgttttc
    44701 tcccttcata tctaacattt gtccaccaac catgccaaaa ctacccgcag acatagacaa
    44761 taactctatt aacctaacag taacatcagc tttaaaacca gcttttacaa ctaaatcaaa
    44821 tgggtctaaa aaaagggaat ctccagctaa aaccgctgta gcttcatcaa actttttatg
    44881 gtttgttaag cggccacgac gaaaatcatc attatccatc gccggtaaat catcatgaat
    44941 taaagagcca gtgtgaatca tctccaaagc agcagccaca tcataatgac catctattag
    45001 ttcaactcca aatccctcta aaatttctaa aaggatgcgt ggacgaatac gtttcccacc
    45061 tccatcaact gagtagagga tagccttaat taaatcagga gaaacgacac tatgtgtctg
    45121 tttgtaatga cgatgaatag cttcatcaat tttttcaata gtaaccatca agtatccatt
    45181 tctacttccg ttccgtcagc ttgcatcact tttacaagcg tctcttcagc ttcttttaaa
    45241 gttttttgta actcttttga aataagcatt cctttttgaa attcagcaat ggcatcttct
    45301 aaggcaacat cacctgtttc taaacgtgag acaattgtct ctaattcctg caaattttct
    45361 tcaaatgttt tcttgtctga catccttcac ctcaacttct aaaagaccat cttgcatctt
    45421 aacttgcaaa tgatctcctt catttattcc attagtagta gatactaaag tatggttttt
    45481 ttcgataata gcgtaaccac gcgctacaat tctagaacta tccaatgata taagcgcatc
    45541 ttgagccttt tcaaaacgtg ctagcttgct gtcatactga ctagtcattg tacttgataa
    45601 tagtcgtcta ctttgcacaa cacgctcttg gtaagtatga agccgctgtt ttaaatctat
    45661 gccttgcagt ttttgatgaa gcaaaccttg cttttgtctt actgtttgta gtttatcacg
    45721 catagaatat gttaactgct gatttaggtt atctaatttt tgaaggaaac catcatatag
    45781 acgctctggt tgcctaaaaa taactgattg ctttgatttt tgcaacctct cctcttttgt
    45841 cctgataaga cgaaggctag actgatacat tctattttca cgttcagtaa tccaagacaa
    45901 gatatcaatt ttagtaaccg ttgtagcaag ctcagctgct gcagttggag ttgctgctct
    45961 gcgatcggct acaaaatctg ctaaagttgt atcagtttca tgtccgacac tagaaataac
    46021 gggaagacga gattcaaaaa tagcttctac aacacattct tcattaaatg cccataagtc
    46081 ctcaatagaa cctcccccac ggccaacaat taatagatcc aaatcctttt tctcgttagc
    46141 taaagcaata gtttgtgcta tctcctgagc tgcgccctct ccttgaactt ttgtggggaa
    46201 aagtaatatt tccactcctg ggaatcgacg agatactgtt gtaataatat cccgaataac
    46261 agctccactt ggactagtca caactcctat ttttctaaca aattgaggaa gtaattgctt
    46321 atgacgatca tcaaagtaac ctgcttgaga gagtttcttc tttagttgtt caaattggat
    46381 agcaagagct cctataccat ccggttctgc tttttcaaca ataatagagt aggagccgct
    46441 tggttcataa agttgaacac ggccaactac attaaccttc atcccttcct ctaattcaaa
    46501 acccagtttt ttgaattgac ctgaccacat tgtcgcctga atgactgatt tatcatcttt
    46561 taaagagaag tattgatggt ttgggcgacg acgaaaatta gacacttgcc cagttaaata
    46621 aaccctctct aaataagggt ctttgtcaaa ttttaatttt aaatacttag tcagtgtcga
    46681 cacggataaa tagtctgaca tagcacctcc tcatgagagt tttattaact tcattatacc
    46741 aaaaaaactt aaaaaatacc tatttgctct acaaatagct cgataaagca aaagaagtgt
    46801 catgctaaca cctctctact tattttttat ttagatagtc tagccatttc ttttggaatc
    46861 tcatcactaa tcgttgtcgg tagaacaacg taggcttctt tagataattc ttgagcaatt
    46921 gccgaatgta gataaactcc cactgatacc ttatctagag gagaggcttc tttaaattgc
    46981 gctaacatac ctgcaatcat accacacaaa gtatccccca tccctccagt cgcctggtaa
    47041 ggacctccta ctataatttc tttttcgtct aaatcttgaa aaatacgcgt atgcgaactc
    47101 ttagcgacta agatcgtccc tttgggaaaa gatttaagag cggtttgggt attttctttt
    47161 gtctgttgcg atacagcaat acctgacaat cgctcccatt ctttttgatg aggtgtcaac
    47221 actaaattct tagcctttgt ttgcggaaac gcaccttctg ataacagagt caaggctgat
    47281 ccgtcaatga tgacactttg atgtgattga atcttctcca tggtccggtt caaaattttt
    47341 cgagatgatt ctgatactcc taatccagga ccaattacaa taacatcact ttgaacaatt
    47401 gatttttcca accatttgta atcatcacta tcaaacgcca ttacctctgg tagttgacta
    47461 tgcaaagagg ggatattgca actttgggtt gctacagtaa ctaatcctgc accagttttg
    47521 acacaggcca aagctgccat tataatagca cctccatagg gataaaaacc tcctatcaga
    47581 agaacactac cataatcacc tttatggctt gatttagggc gtggcttaat taacgccctc
    47641 gcttcttgtt caccaacaat catagactca cacttctaag agctgcttga taagtttgtt
    47701 ccaaaagcat tgtaatcgtc ataggtccta cgcccccagg aacaggtgtt atcatactag
    47761 caacttctgc cacttgttca aatacaacgt ctccaatcaa tttaccattt tcatcgcgat
    47821 tcataccaac atcaatcaca acagcacctt ctttaacgaa gtcttttgta acaaagtgcc
    47881 cctgaccaat cgcaacaata aggatatcag cctcctttgt tacctcagaa aggtttcgag
    47941 ttcttgaatg tgtcaaagta actgttgcgt ttttatcaag gagaagttgt gccattggct
    48001 taccaacaat attggacctt ccgatgataa cagcatgttt tccctctaaa tccacatggt
    48061 actctctaaa catctccata ataccagctg gcgtacaagg taccatcatc ggtcgtcctg
    48121 accaaagatg acccgtattc attgggtgga aaccatcaac atctttctta ggatctattg
    48181 ctaaaataat ttttttatcg ttaatatgtt gtggtaacgg aagttgaact aaaataccat
    48241 ggatactttt atcttcattg tattgatgga taatatcaat cagttcttct tgagaaatgg
    48301 attcagataa tcgtaaagtc tcacttttaa aaccagcttc taatgcagag cgttctttat
    48361 ttcgaacgta cacttggcta gctggattat ccccaacaag gattacagct aaaccaggta
    48421 tgataccatg ctgttccttt aacctttcaa cttttcgccc taactcagcc tgcatttttt
    48481 gtgataaggc tttaccatct attaattcag tcattgatac tccttcttca tccgtttttt
    48541 ctatattata tcaaaaaata gcgattatgc tattttatat cgctattcat tttgtatcaa
    48601 ttttaatcaa tagaaaaggc aatgaatccc tcatcgcctt tttacatatg acttatttag
    48661 tactattcat tttagcacga caagccgact caattgcatt aagtttcgat aaactatctg
    48721 cttctgtaca accaatcgta taaaggtaac attttatctt aggttctgtc cctgacggcc
    48781 ttaaagcata ccatgaaccc tcattaaaat aatattttaa acaattttgc tttggaaaat
    48841 ccttataacc atccttgaaa tcaatagaat tctccaatgt catctcacct acttgtaata
    48901 ttgggttctg tctaaaatcc tccataatac gactaatacg ttcttgcccc tcagcaccct
    48961 ctaactcaag agaaaattgg cgctcgttgt aatagccaaa ttcatcgtaa atggtttgca
    49021 aaacgtctaa aagtgtttgc cctcgttctt tataataggc agtcatttct actaccatca
    49081 ttgaagcact cacagcatct ttatcacgta caaaagtgcc atagcaaaaa ccaatacttt
    49141 cttcatagcc aaagagataa gttttatcct ttgagatatc atattcatta gcttttccac
    49201 aaatattttt aaatcctgtt aaagtttcaa cagtttcaat attatattta tctgcaataa
    49261 cttttgatag atcaccagtt acaatggatt ttaccaatac aggatgatgt ggcaaattcc
    49321 ctaaggcaca tcgttgtgaa aaaatataat aggaaagaag tgcccctatc ttattaccat
    49381 ttaagaaaat atattctccc ttactatctt tgacttccaa tgctactcgg tcacaatctg
    49441 gatctgtggc aagtaagaca tctgcatcaa ctgactttcc tagagattct gaataggcaa
    49501 atgctttagg aacttcaggg tttggatagc caactgttgt aaaatcagga tcgggcattt
    49561 cctgctcagg tactacataa atattttcaa aaccacggcg tcttaaaact tcgcgcacag
    49621 gtaaatttcc tacgccattt aaaggagtat aaactactcg gactgactta tcaatgttag
    49681 tatcattaat agttaaacca agcacttctt ttttatatgc ttcttcaata ctctcatcaa
    49741 tataacttgc caaaccactt gccagagcct cttcaaaggg tatttgctta atttgctgat
    49801 aatcggttat agcatccata tgattggcaa tttggtcagc aatatcatct aaaatctgag
    49861 atccttcttt ccaatatgcc ttataaccat tataagcttg aggattatga ctagcagtaa
    49921 ccatcacacc cgaaacacat cctagagcac gaatagcata tgagcacatt ggtgttgggc
    49981 gaatcccttt ataaatataa gacttaatac catttgctgc cataatggaa caagttaatt
    50041 ctgcaaattc cttagattga taacggacat cataactaac tgcaattcca cgtgcaatag
    50101 cttcagggcc atgatcaata atcgtattag ctaatgcttg tgctgctttc cccaccatat
    50161 aagtattcat acgattggtt cctgctccaa gtttacctct caatcccgcc gttccaaatt
    50221 ctaatgtttt gtaaaaacga tcctgaattt cagattcatc tcctttaata gcttctaaat
    50281 ctgacttaat atctttaccg agtgagtcgt tttctaacca ctcttgataa atctctttat
    50341 aattcatatg agacatccta ttctaagacc aaaatggtaa aaatcagctc catgataaga
    50401 gattttagtt cttcaggagc taactgaata gtatcaaatt aaccttataa agtcaactta
    50461 ttttataaat taactacact taatatattt aaagcatcta aatttttttc atcaccctat
    50521 cattcttaat ctacattata tatagactta ccaacttcca aacaatacag taacaattta
    50581 ctcataacaa tggaataata aaagtctaaa acaagttgtt ttagacaatg aaattataat
    50641 actttactta agaagtctct tgtgcggatt tcttttgtct gctcaaatac tttcttaggg
    50701 gtcccttgct caacaataat ccctgcatcc ataaaaatga cacgatccgc tacttcacgt
    50761 gcaaaaccca tttcatgagt gacaataacc atcgtcatac cagatttagc taaatcttgc
    50821 ataacagtca agacttcacc taccatttca ggatcaagag ctgaagtagg ttcatcaaaa
    50881 agaaggacat caggattcat tgcaagacct cttgcaatag caatccgttg ttgttgtcct
    50941 ccagataagc ttgctggata agcattagcc ttctctttga gtccaacttt ttcaagtagc
    51001 tcgtatgctt ttgtctgagc atcaagctta gaaagtccct ttgtcttaat aggtgataaa
    51061 gtaatatttt ctagtacagt catattggga aatagattga actgttgaaa aaccatgccc
    51121 attttttcgc gcattttaaa aatatcattc tttttgtctg ttatatcaat cccttcaaaa
    51181 gtcactgttc cctttgttgg tacttccaag agattcattg ttcttaaaaa tgttgactta
    51241 ccagagccag aagggccaat aataaccacc acttctcctt gatgaatatc caagtcaatg
    51301 ccttttaaaa cctcattttt cccaaaactt ttatgaagtc cttgtattga aataattggc
    51361 tcaaccatct atttttaccc ctttcccaag atatttctcc atttgtttta acaaagctga
    51421 gagaatcgtt gtcaacatta aatagtaaaa tgctgcaaat aataacggtg caactggtga
    51481 gtaagtagcc gttacaactg attgtgctcc gttccataat tccatgacac caatagtttg
    51541 aaggagagca ctatctttaa taattgtaat aaattcattc cctagagcag gtaaaatatt
    51601 tttaaaagct tggggtaaga taacatagcg aagtgtattt ttaggtcgaa tccccaacga
    51661 gtaagctgct tctatttgtc cagatggtac agcctcaatc cctgcacgta caatttccga
    51721 aatataggca ccactattta aggaaatgat aatgatacca ggtaaaagtc gtgtaaaatc
    51781 taaatctaaa acaccaaagc taattgttgg taaattgtta aaatgcatcc atgcgaaagc
    51841 aatcataatt tgaactacca tcggtgtccc acgaaatacc catacataga aattagctaa
    51901 tattgtgaga aaatgtaaat tagtacgctt tactaaagca attaacacgc ctataatagt
    51961 tccaaaaaaa acaacacatg ttgaaatcat aatggttacc atcacaccat aattaaaata
    52021 ggaccaatat tgtggtaaaa atgaaaaatt cattgacatc tcctaaaaaa gtaattgcca
    52081 atagtctatc acacaaagaa taaaaataca agtttaaata aatatttttg tttttattct
    52141 attttaatgt atatttagat ataaaaaaag accttcaagg tcttttataa ttatcagtat
    52201 aataaatcgt aaatttccat tgcgataagg tcaatgctgt caaatgaata tgattcacgc
    52261 gcttctacat cacgtaatgt gaaaacagga ccgttttcag ggtcattact aaaagatact
    52321 tctgctacaa caacaccttc acgttcaaaa ttacgtttta aattaccacc atcagtcgcc
    52381 attaattcga ggcgattgat gattctcact aaatgtgatt ccatcttact tactcctata
    52441 tattttctag tcttttctat tttaacacaa aagtgaccaa agcaacatga aaagcaacat
    52501 ttttactaac tttttaacga ttttatcgtc taaaatccgc aaataacgat ttttctcagt
    52561 agtcatctat ttttcaattt agcactctaa tatattcagt gctaaaaaca gaactttttt
    52621 cttgatttct atgctttagg tgctataata aaagcataaa gtttgactat atttgactat
    52681 atacacatca atagttaaat gtcataagaa aggggtagcc tatgctctgt caaaactgca
    52741 aactaaacga atcaactatt catttgtata caaatgttaa tggaaaacaa aagcaagttg
    52801 atctttgtca aaattgctac caaattatta aaactgaccc taataatcca cttttttcag
    52861 gtttaaatca tgtttcacat gcgcctggtg gcatcaatcc tttctttgat gatttctttg
    52921 gagatttaaa taatttcaga gcctttaatg gccaagattt accaaacact cctcctacac
    52981 aagcaggagg aaaccgaggt ggaggaaacg gtaatggacg aaataataat cgcaaccaaa
    53041 ctgcaactcc tcctcaagcc aaagggattc tagaagaatt tggtatcaat gtcactgaaa
    53101 tagcccgtca tggtgatatt gaccctgtta ttggacgtga ctcagaaatt attcgtgtta
    53161 tcgaaatcct taatcgtcgt acaaaaaata atcctgtact tattggcgaa cccggcgtag
    53221 ggaaaactgc cgttgttgaa ggacttgctc agaaaattgt tgatggtaat gtgcctcata
    53281 aacttcaagg caaacaagtt attcgtttgg atgttgtgag tctagtacaa ggaacgggca
    53341 ttcgtggtca atttgaagaa cgcatgcaaa agttgatgga agaaattcgt caacgtcaag
    53401 atgttattct attcatagat gaaattcacg aaatagttgg agctggtaca gctggcgaag
    53461 gtagtatgga tgcaggtaat attttaaaac ctgcccttgc acgtggagaa ttacaactag
    53521 taggtgccac tactctcaat gaatatcgta ttatcgaaaa ggacgctgcc ttagaacgcc
    53581 gtatgcaacc tgtaaaagtc gatgaacctt ctgttgaaga aaccattact attttgaaag
    53641 gtatccaaaa aaaatacgaa gattatcatc acgtaaaata taataatgat gccatagaag
    53701 cagctgcagt actatctaat cgttatatcc aagaccgctt tttacctgat aaagcaatag
    53761 acttattaga tgaagctggt tctaaaatga acctaacact aaattttgtt gatccaaaag
    53821 aaattgatca acgtctcatt gaagcagaaa atttaaaagc gcaagcgact cgtgaagaag
    53881 attacgaacg tgcagcttac ttccgtgacc agattgcaaa atataaagaa atgcagcaac
    53941 aaaaggtcga cgatcaagat acacctatta ttaccgaaaa aacaattgag cacatcattg
    54001 aagaaaaaac gaatatccct gttggtgatt taaaagaaaa agaacaatct caattaatta
    54061 atctcgcaga tgacttgaaa cagcatgtga tcggccagga tgacgctgtc attaagattg
    54121 caaaagctat tcgtcgtaat cgagttggtc ttggtagccc aaaccgtcct attggttcct
    54181 ttttatttgt aggaccaacc ggtgttggta aaactgaact ttctaaacaa ctagcaattg
    54241 agctctttgg ttcagctgat agtatgattc gttttgatat gtcagagtac atggaaaagc
    54301 atgctgttgc taaattagtc ggagcgcctc caggatacgt gggatacgag gaagctggac
    54361 aactaactga aaaggttcgt cgaaatcctt actcgctcat ccttctagat gaaattgaaa
    54421 aagctcatcc cgatgtcatg catatgttct tgcaggtcct tgatgacggt cgattaacag
    54481 atggacaagg aagaactgtt agttttaaag ataccattat catcatgacc tcaaatgctg
    54541 gttctggtaa aactgaagca agtgttggct ttggtgcctc acgagaaggt aggacgaatt
    54601 ccgtactagg tcaactaggt aacttcttca gccctgaatt tatgaaccgc tttgacggta
    54661 ttattgaatt caaggcttta gataaagaga atctcctcaa tatcgttgat attatgttat
    54721 ctgacgttaa tgcacgtctc gccatcaatg gtattcatct agatgtcact gataaagtga
    54781 aagaaaaatt ggttgattta ggttacgatc ccaaaatggg agcacgtcca ttacgtcgta
    54841 ccattcaaga acatattgaa gatgctatca cagattacta tcttgagaat ccaagcgaaa
    54901 aagaacttcg tgctattatg actagcaatg gaaatatcat aataaaatct tctaaaaaaa
    54961 ctgaggaaag tacaaaaggt tgagtaagaa actcaacctt ttttcacact cttctcaaaa
    55021 aataatagcc tctataaagc tgtttctctc ctatctcgtc tttaaatttt aatacaatta
    55081 ttaaggaaat attccaaaaa ttttagtata ataaataaaa aataattaaa gcttttaaaa
    55141 tcataaaaca caatcaggag agagaactat gactaatcct actttcggtg aaaaaataga
    55201 taatgtagac tataggtcac gatttggtgt ctatgctatt attccaaatc caactcatga
    55261 taaaattatt ttagtacagg cacctaatgg tgcttggttt cttccaggtg gggaaattga
    55321 agaaaatgaa aatcacctag aagctttaac tcgtgagtta attgaagaat taggttattc
    55381 tgcaacaatt ggccattact atggtcaagc tgatgaatat ttttactcta gacaccgtga
    55441 tacttactac tataatcccg cctatattta tgaagttact gcctatcata aggagcaagc
    55501 gccactagaa gatttcaacc atctagcctg gtttcctatc caagaagcta aagaaaagct
    55561 aaaacgagga agccatagat ggggtgtcca agcttgggaa aaaaatcacc attctaggaa
    55621 ataattcata cctttcttcc taaatagtgt tataataaac ctaataagaa tcagggggaa
    55681 aatagttatg agactaatta atacaaccag tagtcatcct gaactagttc gaaatcaact
    55741 ccaaaataca gatgcaaaat tagtggaagt ctattcagct ggcaacaccg atgtcgtttt
    55801 tacaacagca cctaaacatt atgaattatt aatttcaaac aaatatcgtg ctatcaaaga
    55861 tgaagaatta gaagctattc gtgagttctt cttaaagcgt aaaatagatc aatctattat
    55921 tattcaagag cagatgaaat cactccatac tgccaagtta attgaaattt catatccaac
    55981 aacagcataa ttaaaagaag cctaatcaag gcttcttttt tgatgactac taaacaaata
    56041 tagcattaaa taatttttat taagcttcaa aaccttgagc tactgcttca ggataatatt
    56101 gctcaataat tgctgcacta gcatcacaaa cagctacacc atacgaacgc atccttgtgg
    56161 ttggatcaat acggcgtgag cgttcgcaga cttcaccttc agcgtgttca acagtaaatg
    56221 caacaccttc aaatgatact gagtcagctg gtttgtctgc ttcatcagca attgttaatt
    56281 gtgagacaat catcaagagt gcaatatcgc tatttaacgc tgttaataac gttttcactt
    56341 cttgactagc ataaattgtt aaatgtgctt ctagagattt accaatgact ttagcgttac
    56401 gtgcttcttc gagagctttt tgagcttgtg ttcgtaacgt catgaatgct gaccactctt
    56461 caagaatttc ttcttgacca gagaatgtct gtgcaacagg catttctgct aattgaacaa
    56521 attcctcctc ctcatgttca aggtatgacc aaatttcttc agcagtatgt ggtagaatcg
    56581 gagtcaaaag ttttgtaagt ttaactaaaa tatcgtagaa aacagtttgc atacgacgtc
    56641 gttctggact gttagccgct tcaatgtaaa ccacatcttt tgcaaaatca aggtaaaatg
    56701 ctgatagatc aactgttacg aagttaacga cagctttata aattgccata aaatcataag
    56761 cagcataagc tttgttgatt gtatccacaa cttgattaaa tttaatagtc atataacgat
    56821 caacggcacc taaattttca taggctactg catcttgttt aggattaaaa tcggaagtat
    56881 ttgcaatcaa gaaacgcaat gtatttctga tcttacgata agtttctgat gtttgtttta
    56941 agatatccat tgaaatacga acatcattac ttgaatcaac agaagttacc cacagacgca
    57001 agatttccgc accaaattgt ttttcaacat cactcggaag aattgtattt cctagagatt
    57061 tagacatttt ctcaccttta ccatcaagta caaaaccttg agataagact gctttgtatg
    57121 gagcgtgacc attaacagca actgatgtaa ttagtgatga gttgaaccaa ccacggtatt
    57181 gatcagaacc ttcaagataa agatctgctg gataggaaag gttttcacga gcattcatta
    57241 ctccattcca tgatgaacca gaatcaaacc aaacatccat gatgtctgtt tctttctcaa
    57301 agagaccatt aggtgaacct ggatgagtat aacctgctgg tagaaggtcc ttagcatcac
    57361 gttgccacca aacgattgag ccgtattcag caaataaatc tgcaacgtga tcagtcactt
    57421 cttttgtcat gatagctgta ccatcttctg cataaaaaat tggaagtgga acaccccaag
    57481 cacgttgacg tgagattacc cagtcgccac ggtcacggat catgttataa agacgttttt
    57541 taccccactc aggttggaaa tttgtctttt caatttcatc gaggatttct tgacggaatt
    57601 tcgaaacaga ggcaaaccat tgtggaaccg cacgccaaat gattggcttt ttcgtacgcc
    57661 agtcaaatgg atatgagtgg ttgataactt cgctggctaa aagaagatct cccaattttt
    57721 cttttacaag tggtgtcact ttgtcataga attgaccttc aaaatcagga ccagcatttt
    57781 ccatcatcaa accacgacta tctacggtaa ctacaacatc aagtccatta gcaataccaa
    57841 cgttatagtc atcttcacca aaaccaggag ccgtgtggac aatacctgta ccagaatctg
    57901 ttgtaacatg gtctccaagg ataaccaact cttctacttc tgtatcccat ggatgttctg
    57961 taacaatgtg attaagttct ttaccagtgt gatgagtcac aatttcaaag ttttcccagc
    58021 caaacttagc agcgagacta tctacaagaa cctctgcaag aaggtattta cgctcactac
    58081 ctactggtgc aactacaaca tactccatat ctggtcctac tgttaaaccg cgtgaagctg
    58141 ttacagtaaa tggtgttgtc gtccaaacga cgatataggt atctgtatca agaattccct
    58201 taccatcttt aactttattg gcatagtaga gtgatgtcga atcaatatca tgatattcga
    58261 tttcagcctc agcaagggca gactctgatg accatgacca atacactggt ttagcaccac
    58321 gatagatata tcctttatct gccatagcac cgaaaacacg tacttgatct gcttcataat
    58381 ctggtgtcag tgtaatataa ggattttccc aatcagcaga aacgcccaga cgtttaaaat
    58441 catcacgttg tttgtcaact tggctgagag cataatcacg acacatttca aggtattcag
    58501 ccaagtccat ttctttacgt ttaacccctt ttttagctaa tacttgctca attgggagtc
    58561 catgagtatc ccaaccaggc acataaggag ctcgaaaacc tgacattgat tttgaacgta
    58621 caataatgtc tttagaaatt ttatttagtg catgtcctac atgaatattc ccattagcat
    58681 agggaggtcc gtcatgaagg tgaaaggctg gttttccttc attcaatgct tgacgttttt
    58741 tataaatatc agcttgatcc catgcttctt gccattgagg ttccttattt ggaagccctg
    58801 cacgcattgg aaaagctgtt tgtcctaaat tcaaggtttc ttttaatttc atactaactc
    58861 cttatctcat agtaatagtt atcacaattt tttaaattgc taaataaaaa agctttcatc
    58921 acataaagga cgaaagctcg tggtaccacc ttaattcaga gcagaaaata gctatttctt
    58981 actccctcaa caacccgtaa ggtgggttaa gccttctacc ttacttaaat aatagttcag
    59041 gtagaatgat tgaaaatgat aatttcaata ttagggattg tagggctctc accatctcct
    59101 actcgctgat aatataatat taaaaccttg tttttcttaa tcttcaatat ttaatttaaa
    59161 agtttgagtt tcacctaaat tgatcggttc ttccatttgg aaattattag tatctaataa
    59221 acctgactct tcaagttgtt tgttactttc ttcaacacga cgctgtaatt cttccatttc
    59281 atctggtgag aattgacgag tagcatcaaa tgactcagtg tcatccacca ctggtaaagc
    59341 atcatcttca tcaagaactt tttcaacaac ttctttgaaa gaagcatcag aattttgaag
    59401 ataaatagct gttggtttca acaattcttc ccaagcagat gagttagcta attttaactg
    59461 accttctaat tcagaaagta agcgttggtg gaaaacacgt gattgacgtt tcaaatcttc
    59521 tgtttctata gcgacacgct tagcatcatc tgtcgcatca cgaaggattt gatttgcttt
    59581 taatttagcc tcatcaatta aatgttgagc atcaaatgta gctttcccca ttaggttaga
    59641 tgcttcatcc tgtgctgaaa ttttcacacg ttcagcagtt tcttgagcta aaataactga
    59701 ttgacttaac gattccttca tttcgttgaa gtaagcgatt ttctcttcta aatctttaat
    59761 gtattgctct tgctcacgat tacgtctaat caagtcttcg taatcgtcaa caacaatctc
    59821 taaaaattca ttaacttctt cttcgctata accgcgaaat tttgatgaaa atgttttatc
    59881 tttaatttca agtgctgtaa gtggcatgtt ttctccttag ttatgtatca ttttgtctac
    59941 ttctaactta tatttttgat ttttagttaa ccctaggtta tggttcaacg taaatcgacc
    60001 atatcctcta acactaatca aatccccctc tactaattgt tcagaaactc gattgacgat
    60061 tgcataattg accttgactt tatctgcctc aatcaatttc gtcgactgtg ttcgtgaaat
    60121 ctttaaaatc gttgctaaaa ttttatcaag acgtaaactg gaagcaataa cagttagttt
    60181 ttgaatatct tgtttgggag ttagaagttt ggacaaggga acttcagcta attgtacact
    60241 agcagttcca atttttgtaa ctgaatgaac aaggtgattt gtcatttgac tatcgaccaa
    60301 aacctgagca cagccttctt caactaggat atcccctaaa attgatcgtt ttacacctaa
    60361 gtaattcagt aaagttccca taattttagc atgggtaagg tgattaaatt tagcattata
    60421 ctttatttca attaggctaa ggttaaaatc aaccatatct aactgatagt attccggtgc
    60481 tattattact ttaacatatt ctgtttgaaa atactgacca gaagtgtaat aatgactacc
    60541 tcgcctctct agaacacttt cgagtatttt aaattcccta ggatttagaa attcagtggt
    60601 aataaacgaa taggtgtttt cgacatattg agctaaatgg tctattttat tgataaacgc
    60661 atactcttct ggtcgaaaat gctgataaat atcatctaac gtcatcttat gtaaaaagca
    60721 agattaaaaa tctactcaac atatttagta aaaagaatgc tactattact gtaaaatcta
    60781 gacctaaaaa ttgtagatta aatcttctga gtgggcgaat aatcggatca acaatctgta
    60841 ccactagtct accaatccat gaatcgtatg ctcccggaag ccaagataac aaggcatagg
    60901 ctagtaagat ataagaatag accctaattg tttttaataa tattaatagt actaatatca
    60961 tatttatcgt ctcttcatat caaaatcaaa actcgtctct tgaccagtct ttggaatatt
    61021 catctcctca atatcgacca taacattggc tggtgttaat agaaacattg aactcccaac
    61081 cttttgtaat gaaccatata atactctact tgcaccatca atataatcca agcaacgtcg
    61141 tgcttgcgca tcaagcatat attgaaaatc aatcaagaca cattcattaa cgataagtaa
    61201 atcaacaatt tcttgtgcat cctcatactt acggggatac ttcagagcaa ttgttacttg
    61261 ttctctctgt tgtcgaactg agtctttatt ttgaatccta ggagtagctt gctgataagc
    61321 attgtctctc tgtacccttt gtctctcctg acgattagga ttataggtag aagttctcgg
    61381 acgactacca atcatctctt cctctgcgga gttagtcata tgactacgct gagaagcttc
    61441 ctgtgctgta gctgctctgg aatctctttg cactgaagtt ctctcttgta cttcgtgtac
    61501 ttcattttca cttacatcgt cagtgtcaaa ataagaaata attttgtcaa atctatcttt
    61561 taatgccata ttcccctcca ttatttaaag aatgaagttc ctatccttac aaatgttgat
    61621 ccattttgaa tagcaatatc ataatctcta ctcatgccca tgcttaattc tgtaaacggc
    61681 attcgttcta tgttcctagc ttgaagcctt ttcctaagac tctcagtctc ttcaaaaatt
    61741 gatgatattt cctctttagt tgcttttaag ggagccatag tcatgatgcc gataagttca
    61801 attttatcgt accgagaaat taagttcagt gcatcatcta tttgctcaat agtaaaacca
    61861 tgcttactgt cttctcgtga tatattaact tgtagaaaac atttaactgg tttttgagca
    61921 cgtttttgaa tctcagctgc taacttaaca gaatctaaag catgaaaata atcaacataa
    61981 ttaatgacat ctttaacttt tcgacgttgt aaactaccaa tgagatgcca tgtaagcttt
    62041 tcatctttta atgcttgata tttttcaaga aatttatcaa cacgattttc accgatatga
    62101 ttaacacctg ttctaataag cgcttctgtt gtttgacagt taacatattt tgttacagct
    62161 acgatatgaa ctgattcagg agcgcgacct gcttttagtg ctaatttact aacattgtca
    62221 aaaatcgctg ttttattttc ttgtaaattc atcattttaa ttaacggttt ttaaagaatg
    62281 gaggtgtttc aagttcatca tccatatcat cattttctga aaacgttgac atagacaact
    62341 tgctatcaag ttcaccttct gtcggacgtg aaatattatc acgacgaagg tcccaattac
    62401 caaaagcaga actttgttgc tgattttgtg catgaggctg attttgttga gttggcattt
    62461 cgcgagactc tgtcatatca aagttgccac gacgatcaaa gtttgaattt gaagtacttt
    62521 gacgttctga aggtgcttga ttagttggag ctgatgttgt aaaaccagat acttgattag
    62581 ttttatcttt acgcacacct gttgcaacaa ctgttacgcg gatttcgtct ttcatatcca
    62641 tgtcaattga agtgcctaac caaatattga cacctttacc tgctgcttgg ctaacaatct
    62701 ctgatgcttc ctctgcttca gtaagtgtca tatccatacc gccggtaaca ttgacaataa
    62761 cgtcttctgc tccatcaatc gttgtttcaa gaagtggtga ataaattgct tttcgagcag
    62821 cttcagtaat acgttcctca ccagaaccga taccaattcc catcagtgca ttacctttat
    62881 ttgccataac tgttttaaca tctgcaaagt caaggttaat taaacctggg ttagtaatca
    62941 aatctgtaat accttgaaca ccttgacgta aaacattatc tgcttcacta agagcttcaa
    63001 gcaatggtgt cttcttatct acaatttcaa gaagattatt atttgaaatg ataagcagtg
    63061 tatcaacttg ttctcttaat tcttgaatac cttcgatagc gaagttagaa cgtttattac
    63121 cctcaaagcc aaaaggacgt gtgataaccg ctactgtaag tgctcctaaa ctctttgcaa
    63181 tacgtgcaat aactggagcc gcacctgtac cagatccgcc ccccatacct gctgtgataa
    63241 ataccatatc agcgccagta agtgcttctg ttaaaacttc ttcactttct tcagctgctt
    63301 tacgaccaac ttcaggttgt cctcctgcac caagtccgcg agttaatttt ggaccaagtt
    63361 gaatgacagt ttcagctttt gagctactta aggcttgaat atcagtattt gctgcaataa
    63421 actcaacgcc agcgacacct tcgtcaatca tgcggttaat agcgttaccg ccaccgcctc
    63481 caacaccaat aactttaata actgcacctt gtactgatgc tgtatcaaat gaaaatacca
    63541 tttttattcc tcacttattg ttttagtcaa acatacttcc aaaaattcct ctgatgcgct
    63601 caccaatatt agttttaggc tcagtaggct cttgagcgtt ttctcttgct cggattggtt
    63661 ctaaattaga atcttccata gaagaggtta acggctcaga ataaggcatt gtcgacattg
    63721 tatttgtttt ttctttataa tcaaagtcga ctggtttatg tcttagcatt tcgtcgccag
    63781 ttacagcatg ttgtgcgata atgtcaactt ctgacatcat tcctacataa tcaacgatac
    63841 taataacatt agcgaacatt ggattgcgaa tacctacttg gtttggaacg tgcaatttaa
    63901 ctctagttcc aaaaatttgt tgagcaacct cgacaactcc tggcataatt gcaccacctc
    63961 caacaagaat aataccacca ggtaaatcaa gtaaacgacc tctacccaag tcttgtttga
    64021 catgttctaa aatttgacga atacgtcctg aaataatttg cgaaagatag ctttctgtaa
    64081 tttcaacagg ttcctcgtta ccaacaacat taacttgaac tgtatcagaa gtacttgctt
    64141 cttcaacatt agcttgtcca aagttgaatt tcaatgcttc ggcaatttct actgttgtac
    64201 gtaacacttt agaaatatct ttggtgacat aatcgctacc ttcagaataa atattagtat
    64261 attgcaattc ttgattacgc atagaagcga ctgttgtttg tccgcctccc atatcaatca
    64321 cagttgcacc aaactcacgt tcaccttcgt ttagaactga ttttgctaag gctagagggg
    64381 caatcacaac atgctctact tttatacctg ctcgttcaac agttttacga agattatgaa
    64441 ggatagttgt tggtccagta tagataagac cacgcatctc aaggcgaatc cccatcatcc
    64501 ctctagggtc tctaatccct tggaaaccat ctacaatgaa ttcaagcgga atcaatgaaa
    64561 taacttctct ttcaggagta atgcttttag tcaatgctga tttaacaaca ctttcgacat
    64621 cttcatcctt aatttctttt gattcattgg gaacaggaat cattccttga gttggttcaa
    64681 tttggagaag atttgctggt aatccaacat tgattttgtc aatggtgatg cctgctttct
    64741 cctcagcttg ttttaccgcc tctttgattg cagttgctgc tgcctcaata tcaataatta
    64801 taccatcttt tacaccggaa ctagggacgt tgctaacccc aattacattc atttcatttg
    64861 caataaattc tgcaactaaa actttaatcg agcttgttcc tatatccaaa ccagtaaaaa
    64921 agccatttct agccattcac tcgacctcgc tatctttcca ttcttttcta ttctacatct
    64981 gccaaaaaca gaattattca gaaaatatta taccataaaa aaagcagaat tgttaccaaa
    65041 ttacgttttt ttattaagca acatcaaatt gaaatatttc taacttattt aaaccattcg
    65101 caacttataa caaacttaaa tttttttcaa gaaatcaatt tttactagca atctatgact
    65161 gacttgatat gtataaaaaa gactgataaa tccagtcttt ttttatcgct aattaacatt
    65221 ttgagggttg ggtgcctgct ctgttgctat ctgttgtcct tgttgattag tatttgagtt
    65281 atttgtttgt ccttgactat tttccgcaac ctgaccattt tgtgtttgtg ttttatcagt
    65341 tgatttattt tttgtatctt ccgctttcac aggggttgat tcaatagtac ttgttgttgt
    65401 gtaaactccc acttccatat caacaataga aggttcctta aggttcttct taat
//
LOCUS       DATRNX010000002        51744 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRNX010000002 DATRNX010000000
VERSION     DATRNX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51744
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            64..228
                     /locus_tag="VBC81_000082"
     CDS             64..228
                     /locus_tag="VBC81_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911155.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(211..681)
                     /locus_tag="VBC81_000083"
     CDS             complement(211..681)
                     /locus_tag="VBC81_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911156.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            742..954
                     /locus_tag="VBC81_000084"
     CDS             742..954
                     /locus_tag="VBC81_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911157.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            954..1295
                     /locus_tag="VBC81_000085"
     CDS             954..1295
                     /locus_tag="VBC81_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911158.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            1342..1872
                     /locus_tag="VBC81_000086"
     CDS             1342..1872
                     /locus_tag="VBC81_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6911159.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            1872..2156
                     /locus_tag="VBC81_000087"
     CDS             1872..2156
                     /locus_tag="VBC81_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6911160.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            2180..2398
                     /locus_tag="VBC81_000088"
     CDS             2180..2398
                     /locus_tag="VBC81_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911161.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            2407..3738
                     /gene="dnaB"
                     /locus_tag="VBC81_000089"
     CDS             2407..3738
                     /gene="dnaB"
                     /locus_tag="VBC81_000089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6911162.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            3740..4342
                     /locus_tag="VBC81_000090"
     CDS             3740..4342
                     /locus_tag="VBC81_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911163.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            4320..5066
                     /locus_tag="VBC81_000091"
     CDS             4320..5066
                     /locus_tag="VBC81_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO6911164.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            5067..5888
                     /locus_tag="VBC81_000092"
     CDS             5067..5888
                     /locus_tag="VBC81_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6911165.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            5888..6136
                     /locus_tag="VBC81_000093"
     CDS             5888..6136
                     /locus_tag="VBC81_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911166.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPRPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            6129..6623
                     /locus_tag="VBC81_000094"
     CDS             6129..6623
                     /locus_tag="VBC81_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6911167.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            6620..6949
                     /locus_tag="VBC81_000095"
     CDS             6620..6949
                     /locus_tag="VBC81_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO6911168.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            6949..7368
                     /locus_tag="VBC81_000096"
     CDS             6949..7368
                     /locus_tag="VBC81_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6911169.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            7365..7571
                     /locus_tag="VBC81_000097"
     CDS             7365..7571
                     /locus_tag="VBC81_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911170.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            7561..7977
                     /gene="ssb"
                     /locus_tag="VBC81_000098"
     CDS             7561..7977
                     /gene="ssb"
                     /locus_tag="VBC81_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6911171.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            8558..9877
                     /gene="ltrA"
                     /locus_tag="VBC81_000099"
     CDS             8558..9877
                     /gene="ltrA"
                     /locus_tag="VBC81_000099"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6911172.1"
                     /translation="MIAQKAINIFEKVQVFQRKIYLSTKADNKRKFGVLYDKVYRKDI
                     LKVAWFYVKRNKGSAGIDDFTIEEIEAYGVQKFLDEIEDQLRNKKYQPKAVKRVYIPK
                     ANGKKRPLGIPTVRDRVVQTAVKIVIEPIFEADFQEFSYGFRPKRSANQAIREIYKYL
                     NYGCEWVIDADLKGYFDTIPHDKLLLLVKERVTDKSIIKLLSLWLEAGIMEDNQVRSN
                     ILGTPQGGVISPLLANIYLNALDRYWKNNRLEGRGHDAHLIRYADDFVILCSNNPKKY
                     YQYAKQRIDKLGLTLNEEKTRIVHATEGFDFLGYTLRKSKSHKSGKYKTYYYPSRKSM
                     KSIKGKVKDVIQTGQHLNLPDVMERLNPMLRGWANYFKAGNSKQHFKSIDNYVIYNLT
                     IMLRKKHKKSGKGWREHPPSWYYNYFGLVCLRKLSTNINDDSQRYGR"
     gene            10010..10270
                     /locus_tag="VBC81_000100"
     CDS             10010..10270
                     /locus_tag="VBC81_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911173.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            10267..10614
                     /locus_tag="VBC81_000101"
     CDS             10267..10614
                     /locus_tag="VBC81_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911174.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            10611..11075
                     /locus_tag="VBC81_000102"
     CDS             10611..11075
                     /locus_tag="VBC81_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911175.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            11185..11727
                     /locus_tag="VBC81_000103"
     CDS             11185..11727
                     /locus_tag="VBC81_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6911176.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            12017..12304
                     /locus_tag="VBC81_000104"
     CDS             12017..12304
                     /locus_tag="VBC81_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911177.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            12304..12663
                     /locus_tag="VBC81_000105"
     CDS             12304..12663
                     /locus_tag="VBC81_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6911178.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            12751..13236
                     /locus_tag="VBC81_000106"
     CDS             12751..13236
                     /locus_tag="VBC81_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911179.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            13229..14941
                     /locus_tag="VBC81_000107"
     CDS             13229..14941
                     /locus_tag="VBC81_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6911180.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            14950..16092
                     /locus_tag="VBC81_000108"
     CDS             14950..16092
                     /locus_tag="VBC81_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6911181.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            16142..16684
                     /locus_tag="VBC81_000109"
     CDS             16142..16684
                     /locus_tag="VBC81_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO6911182.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            16695..17957
                     /locus_tag="VBC81_000110"
     CDS             16695..17957
                     /locus_tag="VBC81_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6911183.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPELVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            17978..18313
                     /locus_tag="VBC81_000111"
     CDS             17978..18313
                     /locus_tag="VBC81_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911184.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            18310..18615
                     /locus_tag="VBC81_000112"
     CDS             18310..18615
                     /locus_tag="VBC81_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO6911185.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            18615..18962
                     /locus_tag="VBC81_000113"
     CDS             18615..18962
                     /locus_tag="VBC81_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911186.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            18949..19293
                     /locus_tag="VBC81_000114"
     CDS             18949..19293
                     /locus_tag="VBC81_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6911187.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            19308..19991
                     /locus_tag="VBC81_000115"
     CDS             19308..19991
                     /locus_tag="VBC81_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6911188.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            19991..20467
                     /locus_tag="VBC81_000116"
     CDS             19991..20467
                     /locus_tag="VBC81_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911189.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            20653..23388
                     /locus_tag="VBC81_000117"
     CDS             20653..23388
                     /locus_tag="VBC81_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6911190.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLGAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            23385..24107
                     /locus_tag="VBC81_000118"
     CDS             23385..24107
                     /locus_tag="VBC81_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6911191.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDPDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            24108..27782
                     /locus_tag="VBC81_000119"
     CDS             24108..27782
                     /locus_tag="VBC81_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6911192.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTEAVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKEIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            27799..28029
                     /locus_tag="VBC81_000120"
     CDS             27799..28029
                     /locus_tag="VBC81_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911193.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            28032..28370
                     /locus_tag="VBC81_000121"
     CDS             28032..28370
                     /locus_tag="VBC81_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911194.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            28405..28815
                     /locus_tag="VBC81_000122"
     CDS             28405..28815
                     /locus_tag="VBC81_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911195.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            28818..29147
                     /locus_tag="VBC81_000123"
     CDS             28818..29147
                     /locus_tag="VBC81_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6911196.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            29151..30557
                     /locus_tag="VBC81_000124"
     CDS             29151..30557
                     /locus_tag="VBC81_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6911197.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            30764..30949
                     /locus_tag="VBC81_000125"
     CDS             30764..30949
                     /locus_tag="VBC81_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO6911198.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            31010..31414
                     /locus_tag="VBC81_000126"
     CDS             31010..31414
                     /locus_tag="VBC81_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO6911199.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(31628..31698)
                     /locus_tag="VBC81_000127"
     tRNA            complement(31628..31698)
                     /locus_tag="VBC81_000127"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31664..31666),aa:Cys,seq:gca)
     gene            complement(31832..33364)
                     /gene="ahpF"
                     /locus_tag="VBC81_000128"
     CDS             complement(31832..33364)
                     /gene="ahpF"
                     /locus_tag="VBC81_000128"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6911200.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(33382..33942)
                     /gene="ahpC"
                     /locus_tag="VBC81_000129"
     CDS             complement(33382..33942)
                     /gene="ahpC"
                     /locus_tag="VBC81_000129"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6911201.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            34219..34989
                     /gene="rpsB"
                     /locus_tag="VBC81_000130"
     CDS             34219..34989
                     /gene="rpsB"
                     /locus_tag="VBC81_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6911202.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            35083..36123
                     /locus_tag="VBC81_000131"
     CDS             35083..36123
                     /locus_tag="VBC81_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6911203.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(36282..36743)
                     /locus_tag="VBC81_000132"
     CDS             complement(36282..36743)
                     /locus_tag="VBC81_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6911204.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            36916..37380
                     /locus_tag="VBC81_000133"
     CDS             36916..37380
                     /locus_tag="VBC81_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6911205.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            37377..39824
                     /locus_tag="VBC81_000134"
     CDS             37377..39824
                     /locus_tag="VBC81_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6911206.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(40044..40535)
                     /locus_tag="VBC81_000135"
     CDS             complement(40044..40535)
                     /locus_tag="VBC81_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6911207.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(40549..41190)
                     /locus_tag="VBC81_000136"
     CDS             complement(40549..41190)
                     /locus_tag="VBC81_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6911208.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(41311..42288)
                     /gene="dusB"
                     /locus_tag="VBC81_000137"
     CDS             complement(41311..42288)
                     /gene="dusB"
                     /locus_tag="VBC81_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6911209.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(42272..43147)
                     /gene="hslO"
                     /locus_tag="VBC81_000138"
     CDS             complement(42272..43147)
                     /gene="hslO"
                     /locus_tag="VBC81_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6911210.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(43287..44543)
                     /locus_tag="VBC81_000139"
     CDS             complement(43287..44543)
                     /locus_tag="VBC81_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6911211.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(44540..45358)
                     /locus_tag="VBC81_000140"
     CDS             complement(44540..45358)
                     /locus_tag="VBC81_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911212.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(45482..47734)
                     /gene="gshAB"
                     /locus_tag="VBC81_000141"
     CDS             complement(45482..47734)
                     /gene="gshAB"
                     /locus_tag="VBC81_000141"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6911213.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            complement(47904..48578)
                     /locus_tag="VBC81_000142"
     CDS             complement(47904..48578)
                     /locus_tag="VBC81_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6911214.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIETH"
     gene            48940..49962
                     /locus_tag="VBC81_000143"
     CDS             48940..49962
                     /locus_tag="VBC81_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6911215.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            50333..51625
                     /locus_tag="VBC81_000144"
     CDS             50333..51625
                     /locus_tag="VBC81_000144"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6911216.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
BASE COUNT        16586 a         9213 c        10868 g        15077 t
ORIGIN      
        1 tatttattgt tgtcaagaag ttcgctaact ttttagcaaa aataataaaa ggagggtaga
       61 tgaatgggaa tcggttcaat gacagtgaca gttaaagtta ctaacatgga taaatttatt
      121 gaacttagta aagagttcaa taaaaaagct cgcgagctag aagaactagc tcacgaactg
      181 aagtcatttg attttgaagg cgaagttgta tcaactgata gcaactagct caaatgattc
      241 atcatctgaa aaaagataaa cagggtattc ttctttagtt gcaaaattga agaaatcata
      301 tccaaaatta tcttttttga tgtgggaatt tttttcggag agagagtcaa gattgacctg
      361 agatagtagc ttaaagattt gcacacgagc ttgttcagca tcgttaactc tttgcaaggc
      421 tctcaagtgc tcttcgctag ggaaagctgc tatcaaatca gctctgtcat ctatgtaagt
      481 tctagtgtca tcaagatagc catattttag attatagtct acaaatgacc gctgttcata
      541 ttcttgatag gtttcaaagc tgacaaggtt acctttgtaa cgatagacga gaacgtcatc
      601 ttgtaactct tcttcgaaat ttgtgatgcc aattagaaat ttatggcatg cctctatgat
      661 acgaatttga ttcaaattca tggcttgacc tcctttcata aaagatttca agtcaattat
      721 accaaactag aaaggaaaaa tatgagtcaa caacagaaaa aatggattca gctagtgaaa
      781 gataagctga atgaagagca aatgacacag acacatctag cccgtgcttg tggagtcgca
      841 aaagcgacaa tctcagagct actaaaatac ggaaaaggta gtgtccgtct gaaaaataaa
      901 gtctcagaca ttcttcacat tgatgaaagc tggacggact tggaggaaga ttgatgccga
      961 taacaagaga aatgacaatc actgaaatca atgttctcaa tgctatcaag aatagcgcta
     1021 cttatgatct tccgattcaa gccagagagc taaggcagca gctaggactt agcaaacgat
     1081 cacttgaagc agtcattgag aacctgagag tgatttacaa gcaaccaatc gttgccaaaa
     1141 aaaaacagcc tagcggatac tatcttccaa gaaatgaaga agaaagaaac gacggcttag
     1201 ccccatacaa aagacagata ttgactgagc aaaataatct tgctgctatt atgtctgtca
     1261 atctggaaga atattgggca acagaaaaag cctgacggca atcaggctca caaaatatta
     1321 ttacaagagg attatatcac aatgaacgaa gtttggaaag atataccaat tgttaaagga
     1381 tattatcaag tttccaatct tggtcgtgtt cggtctattg gtagaacagt caatgcaaaa
     1441 cagagaacaa gaaaaacaaa aggacgcatc ttaaaacaga gcttatcttc aggctatgca
     1501 atagtgacac tttcagtcaa tggattaagg aagtcgataa gagttcatag actagtagct
     1561 gaagcattta taccaaatcc aatcaataag cgaacgataa atcatattga tgaaaataaa
     1621 cttaataata gagtagataa tttagaatgg gcgacagata aggaaaatgc taatcatggg
     1681 aatagaacaa cgaaatcatc tttaggtcgt tgtaaacctg tagaacaatt tacacttgaa
     1741 ggggaattca taaacacgtt tgactcaata aaatctgcat caatgaaaac aggtatctcg
     1801 tcacaaagaa taacagcaac agcaatgggg caccaaaaac agactcacgg atataaatgg
     1861 aggtacgcat aatgaatgat ctaatgattc aaatgcttga ccagttcgaa gctggtctaa
     1921 tggacagaac gatgaaagta atgcacgttg tgacagacga aaagtataga tttcctttag
     1981 aactcaacaa gtctcaatgc gctttaatgc tcttaggtac taaagatact gggacatttg
     2041 atgaacgctt taactgtcat aaggattttc cgaggattcc aaatgctcgt gagaaatacc
     2101 ctcgtgatga agtcattgaa tggtatcaca agaattggca aagaactgtt atctaagaaa
     2161 gaacaaaagg aactatatca tgaacaattt acaaattatc gcagtaggca cacttatttc
     2221 agtaaccctg attgagtctc ttatctcaaa tatcaagctc agaaaagctc tcaagaacaa
     2281 acagacagct gggactgtca ttgctcaata tatcccacaa cgaggcttca ttgactacaa
     2341 gactggaaga cgtgttgaca ttgaccctga aacaaagaaa gaagtctttg ttgactgagg
     2401 aaagctatgg atgaattaaa agtattgcct cacgatatcc aggctgagca gtcggttctt
     2461 ggttcaattt ttatcaaacc tgaaaaaatg attgaggtgg ctgagtatct gaaaccaaat
     2521 gacttttaca ggccagctca caagattcta tttaaggcca tggtgagcct tgctgatcgt
     2581 ggcgaggcca tcgatattgt cacaatcaag tcaacgctgg aaagtacgga tgaacttggc
     2641 atggttggcg gtatcagcta catcgctgag attgttaatg cagtgccaac cagctcacac
     2701 gctgagcatt atgccaagat tgttgctaaa aaagcacagc tacggtctat cattgacaac
     2761 ttgtctgact caattggcaa tgcctatgat gaggacatgg atattgatga gattatcgca
     2821 aaagctgagc gatcacttat cgaagttagt caagccagta acaagagtag tttcagacct
     2881 atccatgatg tccttttgga aaatcattca aagattgagg aacgctcaaa caataccagt
     2941 caaatcactg gtattgaaac aggattttat gactttgaca agctgataac aggcttgcat
     3001 gaagaccagc taattgtctt agcggcacgg ccagcgatgg ggaagacagc cctagctctt
     3061 aacatagctc agaatgtggc aactaagtcc aataaggctg tggccgtctt ctcgcttgaa
     3121 atgggggctg aaagccttgt tgagcggatg ttgtcagctg aaggaacaat catcaaccat
     3181 catattagaa caggaaattt gacggttaat gagtggcaac gtcttatcta tgcacagggt
     3241 cagcttgctg aagctccaat ctttatagat gacacagctg gagtcaagat tactgacatc
     3301 agagcaagag ctaggaggct atctcaagag actgacggac ttggattgat agtcattgac
     3361 tatcttcaac tcattcaagg gtcacggtct gacaaccgac agcaagaggt atctgagatt
     3421 tctaggcagt taaagattat tgccaaggaa ttaaaagtgc cagtcattgc actatcacag
     3481 cttagccgtg gggttgagca aagaaacgac aaaaggccta tcatgtccga cttaagggaa
     3541 tcaggcagca ttgagcaaga cgctgatatt gtcgccttcc tctatcgtga tgcttattac
     3601 caagacaaaa aagaaggtca gccagaaaac gacatcactg agctgatcat cagaaagaac
     3661 agacacggaa accttggcac agtcaagctg tatttccaca aggaatatac caagttttct
     3721 agcgttgagg aggaatgaaa tgaaaaaatt tgaccactat ttcagcaacc atattgctgt
     3781 aaaaaacgaa tctgatacag tcaccattgt tgactattac aatccagaaa agggctatga
     3841 gtataactta cgttacattt tcgatagaaa aaattcatcc cttgctatca caggagactt
     3901 tggggaatta gttgcacgaa atttctacaa tatgggtgaa tgggacacat tctacaatca
     3961 ctatactggt catctggggt attttcttga aaagttacta tgttcaagta ggccagttca
     4021 ttactatgat actgacaagg ctaaaagaga aattctcaat gaattctttg atggtgctga
     4081 ttatgatgat ttagattttg atgatgaatt cctacttgat caattatttg acaattttga
     4141 cgaagataaa ggatttactt atattcttcc agaggctttt gaacgtttta gcgaagatat
     4201 ggaagaatat gaggtctatg aatttctatc aagggctggc cgatacgtca atgatgtctt
     4261 tgaactttac ttagacgctt ataagagagc ttatgagtat ctaacaaagg aggttgatga
     4321 tggcacaacg cagaatgttt agtagaaaaa tcactgagac tgaccgtttt ttggaaatgc
     4381 cactatcatc ccaagctctc tatttccatt tgaacatggg agctgatgat gagggcttta
     4441 tagataaggc aaagacgatt cagagaacta ttggcgctag tgatgatgat atgaaattac
     4501 tgattgcaaa agggttcttg attccctttg atagtggtgt agtcgttatc cgtcattggc
     4561 ggattcacaa ctatatccag tctgaccgct ttcaatcaac cttataccag tcagaaaagg
     4621 ctcagctaga gtatgacaag tcaaaaacat ctagtcttaa acctattgga aattgtatac
     4681 aaaatgtatc caaaatggag acacaggtta ggttaagtaa gggtagctta gataaggata
     4741 gcttaactac ctatcctaca gtttcagaca atgaggaaga agatattcca tacaaggaaa
     4801 ttatttctta cttgaatgaa aaagctaata gaaattacag acctaacatt cagaaaaaca
     4861 aaactcttat caaggctaga tggtcagaag gattcaggtt agatgatttc aaacatgtga
     4921 ttgataccac ggtcaaggat tggtcaggca ctaagtatga aaagtatctc agacctgaaa
     4981 cgctctttgg ttctaagttt gaaggctatt tgaatcaagc accacgaatc aagacggaaa
     5041 caattgacgg aaggttgggc ttttagatga atccttttaa gaattttgaa actaggcaag
     5101 tcttggaaga aacctgcgag gttcacggtt gccagctctg gctgactaaa gtaccgatta
     5161 agggacggct tgaagaactc aagcagtgtc cagagtgtac caaggccgct atcaacatct
     5221 ttgaaaataa gttgaacagt cagagcaaaa tcaacagcaa gcttgctgat acatatgctg
     5281 tctttgaaag agatagcttg gtatcagaca agctaagagg caagagcctt gagaactatg
     5341 aaatcaagga tgatattgac cagcaagcta tcaattatgc taagcgtatg gaacagttct
     5401 accgacagga tagaacaggc aatgccattg taactggccc ttctggtgtt ggaaaaagtc
     5461 atctgactta cggcttggcc aagtttatga acgaacaatt caaagcctac gaatcaccaa
     5521 aatcagtcct cttcatctca ctggtgagcc tttttaccaa aatcagagaa agcttcaaag
     5581 ctgacaatgg gtaccgacag gctgagatga ttgagctgct aactagtgtg gactacctct
     5641 ttctggacga tctaggcaag gagagtcgca aaggcgacag ccagaacaat gagtggactc
     5701 atcagattct ctatgagatt ttggacaatc ggagtaacac aatcatcaac acgaatctga
     5761 gcagtaagga gattaaggcc ttatatgctg acaattacgg taatggtgct ctttctagtc
     5821 ggattcttga gggagtgact ggcaatagct ttgcttaccc taaggatatg gaagatagga
     5881 ggtattgatg agctataaag ccacattcat gctatatggt tacaaagctg ttccgattgg
     5941 tctatttgat acaaaggagt tggccgctca agcaattgtt gaccacatca ttgaaaactc
     6001 tggaatacct agacccaggg tagttgccac tatgaaaatc cgaagcaagg aaatcagaat
     6061 tgattatggt tctaaaactt gctactactt gatcacttta attaataata aaacggagga
     6121 aaaacaacat gactaaaaca atgacacttg aagaaaaggt tgaacagtgg tttattgacc
     6181 gtaacttaca tgaggccaat cctgttaagc agtttcagaa gatgatggag gaaactgggg
     6241 agctttactc tggtattgct aaggacaaga gtgaaattat ctatgattcg cttggagata
     6301 tgcaagttgt cctgattggc attgaacaac aaatcaagaa tggcgctcag attgaagcaa
     6361 gtccacaaga tatggagttg ttgctattgg cttcaagttt gggggaaatg gcacagaaac
     6421 tctacaagca tatcttccac aatgaaacaa aaacaccgct tatccgtcca gaattaagct
     6481 tgcttcactc tactattcat gcaatagcta tccacaactt gacaactgct gatgactgcc
     6541 tatcaatcgc ttatgaagaa atcaaagaca gaaaaggcaa gttgatcaat ggcatttggg
     6601 ttaaggagga ggatttgaaa tgaaaaacat gaattggaaa gcattcttca acaatctcaa
     6661 ggcctttgca agtgtcatca tcattactgt atttgttctt ggattcaact atctgtttta
     6721 tgagattggc taccagaaag gccatcagga agctgacagg gtgattatct atgtcgctga
     6781 taatgctggc gctgagatgt ttggcaaaat cactgataaa gaaatcattg aaggccgtca
     6841 cacggttaca gctggcgctt atggaaaatt tctggtcact gaggaacaat acaatgaaat
     6901 caccgttggt gatgacatac cagactacct gaaaggacgt ggaaactaat gacaaaatgt
     6961 aattttgaag caggacacaa accaattgac acaattggac aactagccaa agaagtcctt
     7021 gatgggaaat atggcgatgg tgaggaacga agaagaaatc taggctgttt atacgatcta
     7081 gttcaaacaa gagttaatta tatggttcat gaacgtgaca gcaagttcac aggtatcaag
     7141 atttacgaca atgtgaacaa acctagccat tatcaaggaa agaatggcat ggaagccatc
     7201 gatgttgtca gaaatttcat tggacagctc acaggagtat ctgctttcta ctggggaaac
     7261 tcaatgaagt acctgttacg cttccagggc aagaacggcc ttgaagacct caaaaaagca
     7321 agaaagaacc ttgactggtt gattgaagaa atggagaaag agaaatgaaa aaatcaatag
     7381 gaatccttga atttaaagaa tttgatgctt ttgttggtgg aaaggttatg cctgtctatc
     7441 ttcatagtgc taacccaaac gtgccaacga ctaataaaaa tgaggctatg agggttacca
     7501 aaaagagata cagaaaagag ttctctgaca agtacatttt cagaaaggtt ggtgaggttg
     7561 atgatcaata atgttgtact tattggccgc ttgacaagag atgttgagct acgttacacg
     7621 ccgtcaaaca tcgcaaacgc tacttttaac ctagcagtca atcgaaattt caagaatgct
     7681 gctggtgatc gtgaagctga tttcatcaac tgtgtgatgt ggcgacaaca agctgaaaac
     7741 ttggccaatt ggacgaaaaa aggaatgctg attggtatta ctggacgaat ccagacaaga
     7801 agctacgaaa atcagcaagg tcagcgtatc tatgtgactg aggttgttgc tgacagcttc
     7861 cagattcttg aaaagcgtga taattcaacg aaccaggcaa gcatggatga ccaattgcca
     7921 ccatcatttg gaaatagtca gcctatggat atttcagatg atgatctacc gttttgagtg
     7981 tttcgctaag cttctctcaa tctaacaggt gaaagtcctg tcctaacaat taaccagttc
     8041 ggttaggtag ctatggcata cgtatgcgga gtaatccaaa tgcggaagca atgccgacta
     8101 acgttatccc cagtaggctg atattcattg gtcttatctc agtccgtggg agcgagcaaa
     8161 cctacagtcc gtaacgaaag tgaacttccg ctaagtgtcg ttaaggtcat tccaatgagt
     8221 ataactgaga gagttggaaa tagagttgtc gagcctttag aatgggggcg aagactggaa
     8281 ttgttgtcag ttctaactgg tatatactgg caataaagct ctcggcatat agaagatgga
     8341 atgtaggtaa agatgagcga ggaacgaaag agaccctttt ctatcataca tatcccatgt
     8401 atgtaaggtt agatataagg aaaacaccga agttctaatt gacgtaggaa tagggagtcg
     8461 gagaggttca tagtaccgat aatcatacga gacaacaaaa ctcgatgtag agaaggagcc
     8521 ttgcctttat cggtcttacc gatgaaagag gaactagatg attgctcaga aagctataaa
     8581 catctttgaa aaggttcaag tatttcaacg gaagatatat ctatcgacca aggcagataa
     8641 taagcgaaag tttggtgttt tatacgacaa agtatatcgt aaagatatcc tgaaagttgc
     8701 gtggttctat gtaaaaagga ataaaggttc ggcaggcatt gatgacttca cgattgaaga
     8761 aattgaagcc tacggtgtac agaaatttct tgatgaaata gaagaccagt tgagaaacaa
     8821 gaaatatcaa cctaaggcag taaagcgagt ttatattcca aaagcgaatg gtaaaaagag
     8881 accgttggga attccgacag tccgagatag agttgttcaa acagctgtga aaatagttat
     8941 agaaccgata ttcgaagcgg actttcaaga attttcttat ggttttagac ctaagcgaag
     9001 tgctaaccaa gctataagag agatatataa atatctcaat tatggttgtg agtgggttat
     9061 tgatgccgac ttaaaaggtt actttgacac gatacctcat gataagttac tacttttagt
     9121 taaggaacga gtaactgata agtctattat caagttactt agtttatggc tagaagcagg
     9181 tatcatggaa gacaatcaag tgagaagtaa tattttaggt actccacaag gaggtgtcat
     9241 atcaccgttg ttagcgaata tctacctaaa tgctttagac agatattgga agaacaatcg
     9301 gttagaagga agagggcatg atgcccactt gatacgttat gcggatgact ttgttattct
     9361 atgctcaaat aatccgaaga aatattatca gtatgcgaaa cagcgtattg ataagctagg
     9421 attaacattg aacgaagaaa agacaagaat tgtgcatgcg acagagggat ttgattttct
     9481 aggctatacg cttaggaaat caaaatctca caaaagtggt aagtataaaa cctactacta
     9541 tccttcaaga aaatctatga aatcaataaa aggcaaagta aaagatgtta tccaaacagg
     9601 acaacacctg aaccttcctg atgtcatgga aagattgaat ccaatgttga gaggatgggc
     9661 taattatttc aaagctggga actctaagca acacttcaag agtatagata actatgtcat
     9721 atataatcta acaattatgc ttagaaagaa gcacaagaag tctggaaagg gatggaggga
     9781 acatccgccg tcatggtact ataactactt tggactggtt tgtttaagga agttgagtac
     9841 caatatcaat gatgatagtc agagatatgg tagataactt gtgaatggct ataacgtcga
     9901 agaaagaaga tattgggaaa gccgtgtaag ggaaaacctt acgcacggtt tgacgagggg
     9961 tttctgagga gatggctcaa atcagcgacc tactctacag gaggtgctta tgaacgaata
    10021 taagaaaccc acctacatcg tcattcaaga ggcaatggct gagcgtatta gatttcttga
    10081 agatgaatta tataacagag catacaaaga cattgaacgg ctagaagttg agaatgaccg
    10141 tctgaaggtt agatgccttg atttgcagtt ggaaaatgct gactatgtct gggatgacat
    10201 gtgtcgctct gctattgctc gtgcaaaatc agaaaatcga agaccacaca gaagatggag
    10261 gggaagatga agttaaggct tgaagaacta agaaacaaac ttggcctcag tcgcagagat
    10321 gtgcatgaag ccactggcat ttctatgaac acgctgctac attatgaaaa aggcggtaca
    10381 ccatctattg gtcaggttga aaatatcgct aaggcttaca atatcaaccc agcctggttg
    10441 cttggttgga ttgatgaagg ccagcaacca caaccacttg aaaccgttgt tgaaaagatt
    10501 atctatgtgg aaaaggaagg ctgtagatta ccgccatatt ggaacaatga taataatggc
    10561 agaatcatca agtggaaaga atcaagaaga gcattccaaa ggagaacaat ttgacaaaga
    10621 aaacagcaag taagacaaga cgtgatttcc ttgagtttga gcttgaggct aagtatctaa
    10681 aaattgataa acttattggg cagcgccgtc atgaactgga aagaatctat gcagtcaaaa
    10741 acctgacaat gccaggaatc gatgattcag gagctagtcg cagtggaact tcaactaata
    10801 cttctgaaaa tctagctgtt gcttatgcca gtgacccaat gattctgaaa ctggaagagt
    10861 tccaaaatgc gatatcagaa ttactagagg tacttgaacc cgatgataag aaaattttcc
    10921 atctgaggtg gggagaacat actggatatg attggattca agtttggcac atcatggaaa
    10981 atggcgagac aggttatctt tacagacata gcaagcagat ttatagaaga cgtgaagtca
    11041 tccttgatac acttgcaaaa ttattgttca tgtaacttgt caaaaaaaca tatagaattg
    11101 acagaaacaa tctgatagat tgatagtgtc gctaagcacc gagaaatcct tggtgcttta
    11161 tttttttgag aaaggagcaa agcgatgaat attgttgaac cgctacgaga caaagacgat
    11221 atacaagcca tgaaggacta tctatcatct tggaatgaaa agtattacat gctatttctt
    11281 ttgggcatca atacaggttt ccgtgttgga gatattctca agctaaaggt caaagatgtc
    11341 caaggctggc acatcaaagt tagggaacaa aagactggta agtataaaag tattaagatg
    11401 acaaggccac tcaaaaacga attgagggaa tttgtcaaag acaaggaact tcatgagtat
    11461 ctatttcaga gtcgtgtcgg aaagaacaag gcgctcagtt ataagacggt ttactggttt
    11521 cttaaaagag ctgctgaaga cctcggcatt gacaatgttg gaactcatac aatgcgaaaa
    11581 acctttggct atcattacta caagaagtac aagaacgttg ctgacttgat gtcactattc
    11641 aatcattcaa gcccagcagt tacactaatc tacatttgtg taaggcaaga tgagcttgat
    11701 actaagatga gtaattttag cctctaatat ttttttgatt ttttcaacta tccataacga
    11761 ggaagtttct agtctatatt ttgaagtgag cttaaagcct tgtcctaatt agttttctag
    11821 tgtgaaacaa aattggataa aatataagat atagatagtt cagcatggtt attttacata
    11881 atttcagcca tagaaaaata atcttgtcaa aaaaatgggt attattgaca aaaacaatct
    11941 gatatatttg taacatgaga aaaatccaga gagcaagctt aggcatgttc ttttttttgt
    12001 tgggggtgac gatgccatga aagatgaaaa tgcatttgat ggaattaggc ttgaagctgg
    12061 tagcacttta gaaatctatc tcacgaagaa cgacttagaa catattgcaa atggatatga
    12121 ggtgacttta gatattaagc ctaacgagac tgtaaataaa atcgtaataa agccaagttt
    12181 tgtaaataat attctaaatc cgttaatcaa ctatgacaaa agaatagtga gtgaagcaga
    12241 tttaaagttt agagacattt caagagaggt tgcaagagat agttttgcat tggggtcaat
    12301 gtagtgggct atagaaaccc taaacactct gactggttca gagcttggca gattaaattc
    12361 tacaactcga aaccttggag aactctgaga aataaaatca gaaaagctaa gcgtatgcgt
    12421 tgtgacatgt gcggacgttt gattcacggt aagagtattg ttgaccacat catagagatt
    12481 gacgaaacta attatcaaga tgaatctatc actctcaacg aagagaactg tcagttactt
    12541 tgtcttgaat gtcacaacac aaaaactttt caaagtaaaa taaatttaaa tttagataat
    12601 cggaatataa atttattttt gattttttta tttttgaatt tttgtggact ccccccattt
    12661 tgaattttga cagcgccaaa ataataacgg tgtcaatcct cttgtgtacc tctcccccaa
    12721 aattgacgaa aattgataca agaaaggagc atgattttga aaatcaatga agttttagaa
    12781 aagctaggaa taagtcgtgc tacccttacc aggtatcgaa aaaagctggg catatttgaa
    12841 gaaactaggt caaatatcac caaaagtcag ttcaaagagt tggaaaagct tgccaatcaa
    12901 cggcaaaagt acacaaggca ggaacgtgtt gagctatctc gtaagacttt caagctgatt
    12961 ccaaaagaaa aaatgcttga aatcagtgat aatgattcag ttgtgttgaa aaatctcaaa
    13021 actcaataca atcacaatca aaaagtgatt gaaaacttcc agcttgaaat caataaggtc
    13081 atcaatgacg gtgagctacc tgataagtac ctacttgatg gaatggaaaa gtatcaaaag
    13141 cttaacatgc agatcatgtc aacgattgaa aagcaaagtc cacagggtga cagtctcaaa
    13201 gaaatgattc aggagaagtt agcacgttat ggttgagatg agatattttg ataagtatgc
    13261 tcagctcatc tatactggta agattcgtat ttgtaagctc acaatgaaat caattagacg
    13321 tgttgagcga tacaaagagc aatacctctt caaacaggag gaagctgaca aacggattga
    13381 gttcattgag gaagagtgca gcaatactaa aggccttgct ggtaagttac gcttagcatt
    13441 accacaaaag gtttggttag aaacaacgtg gggcttttat cacacggttg aggttactaa
    13501 gaccaatcct gataccttgg aagaatacac agattatgaa gaaaggcgtc tcattcatga
    13561 ggtgcctatt attgtgcctc gtggcacagg taagactact cttggttctg ctattgctga
    13621 ggttggtcaa atcattgacg gtgagtgggg tgctgatatt cagcttcttg cttacagtcg
    13681 tgaacaggct ggctatttgt tcaatgcctc aagggcgatg ttgtcgaatg aagaaagctt
    13741 gctgcactat atgcgtgagg ctgacatcct acggtcaacc aagcaaggta tcttgtatga
    13801 aacaactaac agtcttatgt ctatcaagac ttctgactat gaaagccttg acggtactaa
    13861 tgctcactac aatatctttg atgaggtgca cacttatgat gatgacttca tcaaggttgt
    13921 gaatgatggt tccagccgta agcgtaagaa ttggataacc tggtacattt ccacaaatgg
    13981 aactaagcgt gacaagctct ttgataagta ttacaacatc tgggtagata tccttgatga
    14041 caagattatc aatgattctg tcatgccttg gatttatcag ttggacgatg tgtcagagat
    14101 tcatgaccct gatatgtggc agaaagctat gccattactt ggtatcacga cagagaaaga
    14161 aaccatcgct cgtgatattg agatgagcaa gaatgatcca gcacaacaag ctgagctgat
    14221 ggctaagact ttcaatcttc ctgtcaacaa ctatcttgct tacttcagca atgaagagtg
    14281 taaaggttgg tcagataagt ttgatgagag tttgtttgtc ggagatgatg aacggaacgc
    14341 ccgttgtgtg attgggattg acttgtcaga tgtcaatgac atctgctcta tctcttttat
    14401 ggttgtgcgt ggggaagaac ggcactatct aaacaagaaa ttcatgccac ggcataccat
    14461 tgagacattg ccaaaggaac tgcgtgataa gtacacggag tgggaattaa gtggcatgct
    14521 gcatgtgcat gaattggact acaatgacca agcctatatc tttgaagagt tacggcagtt
    14581 tatgagtgac aacagaattt tgcctgtggc agtcggttat gaccgctaca atgcaaggga
    14641 acttattcgc ttgtttaacg actactacgg ggatatttgt cacgatattc cccagacggt
    14701 caaatcgtta tcaaatccgc tcaaggttta caaggagaag gctaagatgg gcaaaatcat
    14761 ctttgatgat cctgtggcga catggaatca tgccaacgtc cgtgtcaaaa ttgatgccaa
    14821 taacaatatt tttccaaaca aggaaaaggc aaaagaaaag attgacgtct ttgctagtca
    14881 gctagatgcc tttatctgtt atgaaaattt caaggaagac ttgagctact actttgattg
    14941 aggtgaagaa tgaacaacta tttgaaaaat ttgaaggagg tttttgctag gattttccgt
    15001 ccaaacaatc ggaaatctac aagaacctat cttcaaagaa gtatctccta ctggcgtagg
    15061 aactccatct atttggataa catctacaat aagatttcaa ctgacacagc tcagttaagg
    15121 tttaagcatg tcaagattac tcgtaaccca ggcggtgtcg attcgatggt ttggtatgag
    15181 catagtgatt tggctgaggt gctaacagtt tcaccaaatc cactagaagt gcctgttgtc
    15241 ttttggtcaa atgtaactag ggccatgctg cgtgacggtg tggcggttgt tgtaccacgt
    15301 tggaagaatg gccgactggt tgaaatatgg cttgctaaga agacagtgac ttggacagca
    15361 gagagcgtgg agcttatgct tgatgatgtt gctgttgagc ttcctcttac tgatgtgtgg
    15421 gtatttgaaa atcctaagtt gaatgtcacg gctcaactca atcagattac tgagcttatt
    15481 gacattaact tgaatgcttt gacagaaaag cttagcgatg gcaattctag cttgagaggt
    15541 ttcttaaagc taccaactaa ggcagctgat gaacatttga agcaacaagc cagggaccgt
    15601 gttgatagta tgcttgactt ggccaagaac ggtggcattg cttatcttga gcaaggcgaa
    15661 gaattccagg aacttagcaa agactattct actgcttcta aggaagaatt agagttcttg
    15721 aaatcacagc tttataatgc tcatggtatc aatgagaaat tatttacctg tgactatact
    15781 gaggaacaat atagagccta ctattctagc gtcatgaagt tatatcagcg tgtctattct
    15841 gaggagatta acagaaaata cttcacgaag acagcacgga ctcagggcaa taagttactg
    15901 gtcttctttg atatggctga catgatttca tttaaggatt tggttgaagg tggcttcaag
    15961 tccaaatatg ctggattgat gaactcaaat gagttccgtg aaacctatct tggtttacct
    16021 ggctatgagg gcggtgaagt atttgaaacc aacctgaatg ctgttcgtat cggtgcagaa
    16081 gaatctgagt agaatctaaa gggtgggtgg ttggcatatc tttcacgaaa ggaggtaagc
    16141 aatggaaaaa ctaaaaacct ttgttgtgaa gtcggttgag gatgaatcag ctgactttca
    16201 ttttgaggct tacgcttcaa cctatgacaa tacagacaga gaaggcgatg tgatggccaa
    16261 ggggtgtttt gataacactc tgaaatctaa ggctgttgtc cctatgtgtc ttaatcatga
    16321 ccgtaactgt gttattggta agcatgaatt gtctgtggat gaaaaaggct tgcgaacacg
    16381 ttcaacgttt aatcttagtg atccagaggc taagaaaact tatgacctca tgaaaatggg
    16441 ggcattggat agcctgagta ttgggttctt tattaaagat tatgagccta ttgacgctaa
    16501 gcaaccttat ggcggatgga ttttcaagga agttgaaatc tttgaaattt ctgttgtgac
    16561 cgtgccagct aaccctcaag caactgttga caatattaag gaatttgata tgtcggcggt
    16621 tgataagcga atcgctcagg tgaacatgag gcaagaaatc atgagtaaac ttgcaaaaat
    16681 ttaaaggaga catgatgaaa aaatcactca ttgagctttt ggaagctcgt caaaaagcaa
    16741 ctgatgaact ggctgaggta aagcttaaaa aagccactat tgaagcaaag atgaaatctt
    16801 caaccattga agacgatgac ttggaacagt taaaaactga tgcagaaagt ttggtttctc
    16861 aagcaacagc tattaaggaa acaattgctg ggctggattc tgacattgaa gaaactgaag
    16921 aagaactcag caaagctgct aaaatcatta aagaaaaaca gaaaggtaat acacctatgg
    16981 attatttgaa aaccaaagcc gctgctcttg actttgtccg tattctgatg gacaacgaag
    17041 gtagcgcaaa cagtgcccgt aaagcgtggg aagctaacct tgttgaaaaa ggagtgacta
    17101 acctcactaa gattcttcca gaacttgttc ttatcgctat tcaagatgcc tttactaact
    17161 ataatgggat cttgaaccac gtttcaaaag atccacgtta tgcggtgcgt gttgctcttc
    17221 aaactcaagt atctcaagct aaaggacaca aagctggtaa gactaagaaa gatgaagact
    17281 tcacattctt ggatttcact atcaactctg cgacagttta tatcaaatat gcctttgaat
    17341 attctgactt gaagaaagat acaacaggcg cttacttcaa ctatgtgatg aaagaacttg
    17401 ctcaaggctt catccgaact attgaacgtg ctgttgtcat tggtgatgga aaatctaaca
    17461 gtgctgaaga caaaatcact gaaatcaaat caattgctga agaaactgaa gcgaatctct
    17521 ttgaaacaca agaaatcaat gttgctggtg tatttgacaa tgctgttctt gaaacgctgg
    17581 ttgctggtat tgataagatg gttccaaata ctactccaat tcttgtcact tcaaaagcca
    17641 ttgctcgtaa attgaaattg gtcaaagatg cagaaggtcg ctacatcgat cctcaaccat
    17701 ttgctccaat tgcaaccaac ggaaatgtta ttgctggttt tcaagtttac atctatgact
    17761 ggatggacgg tgcaactaac ccaatcattg catttgctga ccaagcttat aagatgattg
    17821 gtgatgaggc tgccgctgac cgctttgatg attatgatgt aacagtcaac cgccgtcata
    17881 ttgagcttgc tagtgttatg ggtggtcgtt tggctcagta caaatcagcg gttaagttta
    17941 caaaccaagc aggttaaata tagaaagggg agtctgaatg acaatcctta aaacaatcaa
    18001 agaaatggta gaagttgacg ttgaagagga tatctttgac gtccaacttt tacgatatat
    18061 caacagtggg atttcatatt tacaaagaaa tgctattcct gttatccgta tcgataaaga
    18121 aacggagtta gagggatgga cagacattaa ggaagaagat agggagacag tgcttgactg
    18181 gttacatctg agatgcgtcc aacgttttga caaatctttg atgacaggca gtgctgctac
    18241 tatggaatgg attgacggag agatgacaaa tcttttgtat caactcaagg caatctatga
    18301 agtgaagaca tgaaatcttc aagagtagct atcattcttt gctatgatga gcgtgttgag
    18361 gttgaaaaag gtgtctggga gaaaaagacc gtagaaaaga aagtcaaagc taagaaagag
    18421 aaaatctatc agcgaaggct tgataaagct atggcagacg gtcaagttct gactgctagg
    18481 tttagtgttc gttccaacta tgtgactgac actcttgact atgtcaaata taatggcaaa
    18541 gagtacaagg ttaacattgg gacagaatca gatgatggtc attacactat catcgaactt
    18601 ggagaactga aataatggcc aagaaatttt tcactagaca agatattcaa tctatccttg
    18661 aaacaaaccc tttgaaggct aaggtcttct acatggaacg tgaggagaaa tcttctcctg
    18721 acaatgtcat cttgtattat cggttaacac caggcagtag cattactgct gatgatagag
    18781 tgcacatgag aaaagtgact ttgcaagtta gtcactacca caagaagaaa ctagacagca
    18841 ttgaaaattt gatgctgtct aattttatgt gtgagccaaa tcaattgaat ctaaaacaac
    18901 ctgacactga ctatctactg acaacttaca gacttgaggt gtttacaaat gggcggtggt
    18961 agcgtgaaga tgacaccgct aaaggtggat atcaagaatc aagttttaga gaacattaag
    19021 aaggctgccc aaagtacaga aaatgacata agaaccaaaa gccctagacg aaacggtgtt
    19081 tacgaaaaag gttggacaca cgacatcata gatgacgccg ctgttgttca taataatggc
    19141 aaagaaaaat ctctatccca cctattagag aatggtcatg ccactaaaaa cggtggcttt
    19201 gttgccccta gagagcatat cagaccagcc tacatcaaga ataaggagaa attccttaat
    19261 gatatgaaat ccattaaaat cacacctaaa taagaaagga gtcttacatg acttatcaat
    19321 acgatacaag ggaagtcact cacggtaatg cgaatggttt ttatgccaaa attgctaaaa
    19381 ctgatgctgg tgccttagac ttgcagaaac cttatccctt tacagggcta cgaagcacat
    19441 cctttgaaac ttctcaggaa tcaaatgcct actatgcaga caatgtggaa catgttcgtt
    19501 tgcaaggtaa aaaatccaca gaagggtcta ttacaaccta tcagattcct aaacagttta
    19561 tgattgatca cttaggtaaa aagcttacga actctacacc accagctttg attgatactg
    19621 gtgtcaatac taacttcatt tggggttatg ctgaaactgt tacagatgag tttggagctg
    19681 agattgaaga gttccacatt tggacgaacg ttaaggcatc tgcacctaaa ggcagcacat
    19741 caactgatga aacatcagcg acaccaaaag aaattgaaat cccttgtact gcctctccaa
    19801 ataacttcat tgtggattct gaaaagaaac ctgtatcaga aattgtatgg cgtgatgaca
    19861 gcaaaggaac cgtccgtggg aagtttgaca agttgtttgc tgacaagtca ccaggcaagc
    19921 tgattgattt catcaatgaa gcgcttggaa caaccactac acctaccaca actgttagcg
    19981 gaggctctta atgattaaaa aagaactatc attcacagcg tttgatagtt acggtgaaga
    20041 aagagagcac actgaaacag tgcgctttct ttactcttta ccagctatca agatgtatga
    20101 acagcgaact ggtcgtaact tctttgatga taaccaaaaa gcacttacag catatacaca
    20161 acttgctctt gcaactggtg taaacggtaa cctatctgat ttaactgatg aagagaaaat
    20221 caagatgatg cctctactta tggaacctga tttcatgaac ttcttgactg aagtcattcc
    20281 ttgcctttat ggtgaggttg aaaatggacg tttagtacaa aatgagctta ccgctgaaac
    20341 agcttcatta gctccttggt ttggtgattt gattgatatt ggtttcttct cagacctctt
    20401 ctatgaattt aaccgaagca gagcaaaggt gccgcaagat aaaaaaaagc cttttcagaa
    20461 gttgtaactt ccgaaaaggt ttataaggtt atctttgaaa atcggatgga tgttttttgg
    20521 gcagaatccc aacactttaa ctatttgatg ggaacacttc accaaatgag catcaatcag
    20581 gaagagaaga aaaccttatc caacgctgat ttacttagcg tgatgtcaga ttaaaaagaa
    20641 aggaggaaat ctatgactga aacgtttgaa ggcttatacg tcaaatttgg tgctaatact
    20701 gttgaatttg ataggtctgt aaaaggtatc aacactgcct tatctagttt gaaaaaagac
    20761 ttcaataaca tcaacagaca attgaagatg gatccagaca atgttgactt gttgaatcgt
    20821 aagttggtta acttgcaaga acaggctcgt gttggtgcta tgaaaattgc tgaactcaaa
    20881 aagcaacaga aggcactggg agaatctgaa gttgggtcag cacagtggaa taagcttcaa
    20941 cttgaaattg ctaaggttga atcacagatg aagattgttg ataaggcaat ggagtcaaca
    21001 aagaaacaca ttgaagatgt aggagaccca aagtctattc tgaatcttaa caaagaactt
    21061 gataatgttg ctaaagagct tgatattgtc aatcaaaagc ttgagctaga cactgacaat
    21121 gtcgaactag cagagcaaaa aatgaaacta cttggtaaac agtcggaatt ggttggggat
    21181 aaagtccaag aattaaagaa aaaacaagct gcccttggcg atgagaaaat aggtacagaa
    21241 gaatggcgtc aacttcaaaa tgaaatcggt caagctgaag ttgaagttct aaagattgac
    21301 cgtgcaatgg acattcttgg tgagtcaagc cgttctgcaa ctggagacat caaagaggca
    21361 accagctatt taagagctga tgtcatgatg gatgttgcag ataaggctgg tcagattggc
    21421 cagaaaatgg ttgacgctgg gaaaatgaca gtagatgctt ggtctgagat agatgaggct
    21481 ctggacaccg tcacaaccaa aactggtctg actggtgatg ccttagcaga gcttcaggaa
    21541 attgctaaag acattgctac tggtatgcct accagctttc agaatgctgg tgatgccgtt
    21601 ggggaattga atactcagtt cggtttgact ggggaaaagc tgaaatcagc atctgaatta
    21661 cttatcaagt atgctgagat taacgaaaca gacatttcaa gctctgccat ttctgcaaaa
    21721 caagctattg aagcttacgg tttgacagct gaagacttgg gaatggtctt agacaatgtg
    21781 accaaagccg ctcaagatac aggacagtca gttgacacga ttgttcaaaa agccattgac
    21841 ggtgctcctc agattaaagg tttgggactt tcttttgaag aaggtgctgc actgatcggt
    21901 aagtttgaga aaagcggtgt ggattcatct gctgctctat cctctctatc gaaagctgct
    21961 gtcatctatg ctaaagacgg taagactctg acagatggat tgaatgagac tgttagtgct
    22021 attcaaaatt ctactagtga gacagaggct ttaagtattg cctcagaaat ctttggtagt
    22081 aaggctgctc ctagaatggt cgatgctatt cagcgtggtg cttttagctt cgatgactta
    22141 gctgaagcag ctaaaagttc ctctggtact gtctccacca catttgatga gacgcttgac
    22201 ccaatagata agttgactca gtattctaac caagcaaaag agggaatggc agaacttggc
    22261 ggtaaattgc ttgagactgt catcccagct ttagaacctt tgatgggtat gcttgaatct
    22321 tctgtcaatt ggtttactag cctaaacgaa actgatcaac agactatcgt gattcttggc
    22381 ctagttacaa ctgctgtgat gatgttgctt ggtgcaattg caccgctggt catcgccata
    22441 ggggcaatag gtgcgcctgt cggaattgta gtggcggcaa tagtaggggc tattgccgtc
    22501 ataacactta tcatccaagc aatcatgaac tggggagcca taactgaatg gcttcagtca
    22561 acgtgggatt cttgtgctgc ctggctttct gaattgtgga ctaacatagt cacgactgcc
    22621 accacagcgt ggtcaaattt cactgcctgg ctttctggcc tttggtcttc agtagtctca
    22681 actggacagt ctttgtggtc tagctttact agttccttgt ccaatatttt ctcaagtttg
    22741 attacaggtg ctcagtctct gtggtcaagt ttcacttcca ctctttccaa tttgtggtct
    22801 ggactggtct caactgggtc aaatttgttt aataatttga gtagcacgat ttcaggaatt
    22861 tttaatggga tactttcaac agcaagcaat atttggaatt ccataaaatc cactatttcc
    22921 aatgcaatag atggggcgaa aaatgcagtg tccaacgggg tcaatgccat caagaatctg
    22981 tttaacttcc agattaaatg gcctcatatt ccactacctc acttccgtgt gagtggttct
    23041 gctaaccctc tggattggct aaaaggtggc ttaccaagta tcggcattga ctggtatgcc
    23101 aagggcggta tcatgaccaa accaacccta tttggcatga atggaaaccg tgcaatggtt
    23161 ggcggtgagg ctggcgctga agccatcttg ccattgaata agtcaaccct gggggcaatt
    23221 ggtcaaagta ttgctaacac gatgaataca tcgaacaata ttaacgttaa cttctctggc
    23281 gtcactatca gggaagaagc tgaccttaac agactagcca acgtggttgg aaatcgtatt
    23341 gctgaagaat tgcaacgtaa aactaatttg agaggaggaa tggcatgaca aaaatcaatg
    23401 aacttaccat tgacggtgtg aaaacatcat cttttaagtg tgatgtattg gttgaaacta
    23461 gaccaaatgt cattgtctcc agctccaaaa cagctctatt agagcatgat ggtattagtg
    23521 gtgcagttgt gcaatcgaat agacaccgtg ggctaattga gaagccttat catatcactt
    23581 taattgagcc aagcgatgaa gaaatttatc gcttttctgc tcttttgaac cgtgaaaagt
    23641 tttggttgga aaatgaacag gaaccaacta ttaggctttg gtgttataag gttgatagct
    23701 ttgagattgg aaaagacgaa tttggtgcct gggtggtcga tgttaccttc atctgccacc
    23761 ctaccaagtt ttttaaaaca actgacaccc agactttgac tgggaatggg gttttgaggg
    23821 tgcaagggtc agctcttgct ttcccgaaga ttacagtggt tggtcaaagc gcttctgaga
    23881 catcgtttac ggtggggaat caagtgatta aacttgaaaa gctctcagaa tcgcttgtga
    23941 tgaccaacga tcctgacaat cctagcttta agacggctag tggcaagctt attaagtggg
    24001 ctggtgattt tatcacagtt gatactgcta aggggcagaa tgtcggtgtt gttttaggtg
    24061 ctggcataac gtccttgaaa tttgaaacag tttgggggtg ggcatagttg ctctttttac
    24121 ttgataaaga tgttagaacg gtcaaatgga atggcattcc acttcacgag gccagctctg
    24181 ccattgtcaa agaagaaacc aacagtgatt tctacctgac tgttcgctat cctatcacgg
    24241 attcaggtat ctatcaactt atcaaagagg atatgctgat taaggcgcct acgcctgtgc
    24301 tgggtgctca gctgtttaga atcaagaaac ctattgagaa tgatgatagc ttggatatca
    24361 ctgcttatca tgtttctgac gacatcatga agcggtctat cactcctgta agtgtagttg
    24421 gtcaaggttg tgctatggca ctgtctcaga tggttcaaaa tgctaagact ggtctaggtg
    24481 atttttcgtt caccagcgat attatggaca gcagaacctt caacacgaca gagacgaaaa
    24541 cactgtattc agtccttatg gacggtaaac acagcattgt tggaacgtgg gagggtgagc
    24601 ttgtccgtga caattttgct ctttctatca agcgtagccg tggggctgat cgtggtgttg
    24661 tcatcacaac acacaagaac ctcaaatcct atcaacgaac caagaactct cagggtgttg
    24721 tcacaaggat tcatgcacgg tcaaccttca aacctgatgg agctgaagat gaagtgacgc
    24781 tcagagtgtc tgttgacagt ccactaataa actcttatcc ctacatcaat gagaaagagt
    24841 atgagaacaa caacgctgaa accgttgaag accttagaaa gtgggctgag gctaagttta
    24901 caaatgaggg cattgataag gtttctgatg ccattgagat tgaagcctat gagcttgacg
    24961 gtcaagttgt aaatctgggc gacacggtta acctcaagag tagaaagcac agtgctgacc
    25021 tctacaagaa ggccattgcc tacgagttta acgctttaac ggaagagtat atctccataa
    25081 cttttgatga taagccagga gttggtggtt ctggggtgtc aagtggcttg tctaatgttg
    25141 ctgatgctat acttgtagca agtgccacag ctcaggacgt tgctgttcaa agggctgtga
    25201 aaaatgctaa cgctgccttt gatgcagagt ttggtaaaac aaaaacaaaa attaatgatg
    25261 atatcgaaat cgcaaaagcc aaagtagaaa gctttaaatc ggaactatct aatcgcatgg
    25321 ataatcagct tcttcccctt gcgacagaag ctaagaattt agccagtcag gcacaagcag
    25381 acctgaccag aaaagaaata gagcttcgag ctgaattaaa tagacaagtc acaagcacag
    25441 aagctgtaaa aatctctcta acaaatctta gtcacaatat ggatatcatt aagcaaaaag
    25501 ccctaaacga ccttagagac gcagagacaa ggctaaagga agctgatagc gtccaacaac
    25561 ttgcgacaaa gcgggttgag gataaactga caggacttag cacgaaactg gagtcattct
    25621 ctgttggtgg ctataattat gtcattgacg gcggagaacc taaagaacta atggctaact
    25681 tctatggtaa gacctatgat attaatcccc aactactcga acgaactagt caagcgacct
    25741 tgagttttag ctatgaagca gaatcaacta gtcgtttaga agttagactt tataaaaaga
    25801 tgcacaccgg ggacactagt aagattacaa ttattgtcat gcctaacttt gacttatctc
    25861 cagggaaagg atttatctct cagagctttg atttaggtgg tgttatgcct gaccctagaa
    25921 accaagcctg gcttgtgatg cgggggacaa atgctaaccc cttgacgctc tcgaaagtta
    25981 agctggaacg tggtaccgtt gctacagact ggaataatcg cgatgaaacc ttaaaagcta
    26041 gcttcgccga atacaagcag accgttgatg aaaacttagc caaccttaga acaagtacgg
    26101 aaaccttggc aggtcaactg acaagtgctg agtctagcat taggcagacg tcggaatcct
    26161 ttagcaatcg tttggttagt cttgagacct ataaagatag tgaatcaaat cgagctagtc
    26221 gttactttga agcaagcaag tctgaaaccg ctaagcagct atcagcctta cgaacagaag
    26281 ttaatggctc ttatgtggat aagtcaacct atgaggaaaa tgcaagaggt ttgacaagac
    26341 gctttgaaag tctgtcatca gggacacaga actatgcttt ggggactgaa agagatgtct
    26401 atctaaacgt ttcagggact accgtctatg acctttatag cttcggcact accttagcaa
    26461 taaaagagat tgaaatggga gatactgtaa ctattgcatt taactatttt gcggatagtc
    26521 ggacggcttt tggttcttat gaactggagc tatacggaca tgcaggcaaa atcgaacggg
    26581 ttggtgaggt acagagaacg atatcaaatc gtggtaccta taccgcatct tttgtggcaa
    26641 acaatgccaa tttcagggca aactccctta aaattagatt tacggatagc cgacttaagt
    26701 ttagagtgac aacacttcgt gtgactaagg ggacaattcc tgcggactgg agtccatctc
    26761 cagatgacct aaaagcctac tcggatacta agcttgaaca gacagctaat gagattaaag
    26821 ctagtgtaac aagtcttgat cataaaactc ttaagcaaac tgatattact atgacttctg
    26881 aagggattgt cctaagagca ggcaagacga gtaatgatgt ggcaagagct attggctctt
    26941 actttaaggt cactcctgat gctatagctc tgtttagctc actgataaaa gttagcggga
    27001 acatgttggt tgatggttct gtaactagtc gtaatttggt aacaggcgct gtcgaaacgg
    27061 gacatgttaa ggctggagca atcacaggag tgcttttagc agctgaagct gtaacggcag
    27121 aaaaacttaa agtcgaccaa gctttcttta acaagttgat ggccaacgat gcctacttga
    27181 agcaactatt tgccaagtca gccttcatca ctcaggttca atccgtgaca atatctgcca
    27241 gtcagatttc gggcggtgtt attaaagctt tgaataatgc catggaaatc cagatgaaca
    27301 gtggtcagat actttactac actgaccaag ctgcactgaa acgggttttg agtggctatc
    27361 ctactcagtt tgttaagttt gcaactggta cagtatctgg taaaggaaat gctggtgtta
    27421 cggtcattgg ttctaaccgc tacggtacag aatcaaccaa tgatggtgga tttgttggag
    27481 tccgtgcatg gaacggctca aacattgact cacttgactt agttggtgat aaaatccgat
    27541 tagcaagctc tgcctttgac aattcagatg gttgggatgt tcggacactt gattcaggct
    27601 tgaaaatcac accacacaat agggctgctg aacgcaatag ccgaattgag gttggtgatg
    27661 tctggatttt aaaaggaaat ggatcctatt cttctctcag agatattttg aactcattca
    27721 atgagaactt ctcaaaaggc ccaaatgctg actcttacac ttactaccat tcggggttct
    27781 aactagaaag gaacaatcat gaaccaagaa caacaactca atcaagcgct acgcttgacc
    27841 gtcaatgaat tgacagccca gttggccaat gagtcaacca ctaagaactt gctggctatc
    27901 cagctgactg aagttgatca ggagaaacag caactgactc agcaaaatgc tgaacttcaa
    27961 gcaagagttt ccgaactaga aggcttgctt gatgaacaaa ctcaaccaga aatcattgaa
    28021 ggagaataac catgactaca acagaaaata atttacttga ccttgaaaca atcacagaac
    28081 cctttgactt ggcgacagca ctcaagtaca tgaaggaaaa tggtgagtta atccgttgca
    28141 agaatgctgt caatgacttc tacatgtacc gtgacatgca gaaacgtcct gtcatcgtca
    28201 atggtcgccg tcaattcaag gatgtggaaa ctgtctgggc atttaaccaa tggggcggga
    28261 caacacccac aatcaacatg gctgatctct ttaatcttga atactacatc atgacctttg
    28321 atgagaacgg aaatcctgac tggactgagc ctcacttgga ggataagtaa gctcatctaa
    28381 aataaacagg ataggagata gtgaatgcca ccatggttaa tagatagcac tgtggttgtg
    28441 gcgatggtga cggtgctggg cggtctgttc agcactatta tcaccacttc agccaatcgg
    28501 aaagaccagc tgataaaaca tcagtatgag gatatcaagg aagatttgtc tggcttgatt
    28561 gacaaggtta agacgattga tcatacaacc acggaaacaa agaaaatttc agaaatcaca
    28621 aaagacggta cgcttaaaat ccagcgttac cgtctttttc acgatttgac aaaggaaatt
    28681 tctcaaggct acacaactat tgaacatttc agggagttga gcattctctt tgagagttat
    28741 caactgcttg gcggaaatgg ggaaattgaa gccttgtttg agaaattcaa acagttacca
    28801 attgaggagg actaaacatg acacaaatta cagatattat catcagctca gctatgggaa
    28861 ttttgactat tttggcagga attgcagtgc aagctgtcaa ggtttatttg attaaaaaag
    28921 gcggtgaaaa ggccgtcttg attacagaaa tcttggccaa gaacgctgtt aatgctgtgg
    28981 agcaagtggc cactgaaact ggcttcaaag gagctgacaa gctaacttct gctaaggctc
    29041 agattttagc tgagttgcaa aaatacaata tccacatgtc agacgatgat ctcactttgt
    29101 ttgttgaatc atctgtcaaa caaatgcacg atgcctggaa ggagtaacct atggaaatca
    29161 acacagaaac agccattgcc tggatgtctg cacgtcaagg caaggtcagc tattccatgg
    29221 actaccgtga cggacctaac agctatgact gttccagctc tgtctattat gctctcaggt
    29281 cagctggtgc aagctcagca ggttgggcgg tcaacactga gtacatgcac gattggctga
    29341 ttaaaaacgg ttatgagctt attgctgaaa acgtggattg gaatgctgtc cgtggtgata
    29401 tagcaatttg gggcatgcga gggcactcaa gcggagctgg tggtcatgta gtcatgttta
    29461 ttgaccctga aaatatcatt cactgtaact gggcaaataa tggcatcaca gtgaacaact
    29521 acaatcagac agcggctgct agtggctgga tgtattgcta cgtttaccgt ttgaaaagcg
    29581 gagcttctac ccaaggaaaa agccttgata ccttggtcaa ggaaaccctt gctggtaact
    29641 acggtaatgg cgaagcacgc aaggcagtgc ttggcaatca atatgaggct gttatggcag
    29701 tcatcaatgg caaaactacg actaatcaaa agactgttga ccaacttgct caagaggtaa
    29761 tcgctggcaa acatggcaac ggtgaagctc gtaaaaagtc gcttggtagt caatatgatg
    29821 cagttcagaa acgagtgacg gaattgctca aaaaacagcc ctctgagccg tctaaggctc
    29881 aagaggtaaa caagcccacg gaaaccaaaa caagccaaac agagctaact ggacaagcca
    29941 cagccaccaa agaagagggc gacctctctt tcaatgggac tatcttgaaa aaagcggtgc
    30001 tggacaagat tctgggcaac tgtaaaaagc atgatattct tccaagttat gcactgacta
    30061 tcctacacta tgaaggtctt tggggaactt cagcagttgg gaaggcagat aataactggg
    30121 gcggtatgac ctggactggt caaggcaacc gtccaagcgg tgtcactgtc acacaaggtt
    30181 ccgcacgtcc ttcaaatgag ggcggtcact atatgcacta tgcaagcgtg gatgactttc
    30241 tgacagactg gttttatctt ctaagggctg gtggctctta caaggtgtct ggtgctaaaa
    30301 ccttcagtga ggctatcaag ggaatgttta aagttggcgg tgctgtctat gattatgctg
    30361 caagcggctt tgatagctat attgtcggag cttcaagccg cctcaaggct attgaggcag
    30421 aaaatggttc actggacaag tttgataaaa ctaccgacat tggtgacggt agcaaagaca
    30481 agattgacat tactattgaa ggtattgaag ttaccatcaa cggtatcact tatgaactga
    30541 ctaaaaagcc agtttagaat tagatacgat aagacaaaat aagccctcag caattgccgg
    30601 gggctgtttt tccgttataa cggactttcc tcaaaatatc ggttataatg gacttttttt
    30661 gataaaaatt ttaaaaatat ttgttaaaaa caagtgtttt ctattgacaa gtgtttaaaa
    30721 caagtgtata atacaattaa agataaggaa aggagataag ccaatgacag agcgagagct
    30781 taagaaaatt gctaagaagc aaggttttag taaaactaac tttggcaaag ggtcacacga
    30841 agtttggaaa catccagacg gacggacagt gacgatacct aagccaaagg aaaaagatta
    30901 cagaccaggc acactgaaca acattctcaa agtcttgtat ggggagtgag gacgctcctc
    30961 cccgtacccc taaaagggga tactctgatc attggcttaa tctaatatta tgaaatacac
    31021 ttatttagca ttgtttgaag tggataaaga aaacggtggc tataacattt ctttccctga
    31081 ttttcctgga gcatttagtg aagctgatag cttgaacgag gccattttta acgctcgtga
    31141 agtccttgaa atctatacaa ttatgtttga agacgagggc aaagagtttc ctaaagcatc
    31201 atcattcaag gctcttgcaa gcaatttagc aagtgacgaa gatgtgattc aggctatttc
    31261 agttgatact gagcttgtcc gtgagcgtga acgctctaag attgtcaaca agactgtcac
    31321 actgccaagc tggcttgtgg aagttggaaa agaaaacaag gtcaacttta gccagctgtt
    31381 gcaaaaagca atccgtgagg aattgcaagt ataagaaaga gcggtcaaga tggccgcttt
    31441 tttctgtata aaaaattatg aaaatagata gtaatacttt tcaaaaattg acctatgaaa
    31501 aaccgtccgc cccaaattcg ccccaaatta tttttaaagt tagcctgatt taacctgatg
    31561 gaaaatcaaa aaagcctata aaatcaggct tttcattcgg ttaattcctg ttaattcagg
    31621 tactgaaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccgtgcctta
    31681 ccacttggct ataccgccat aacagataat attctatcga aaaaaaaaag attcgtcaag
    31741 tctctttcgt aaaatagcca attttagccc ttaaacttcc tcatattacc aaaaaaagag
    31801 attgatacaa tgggtatcaa tctcttttag attattgtct gattaaataa tcaaatgcgc
    31861 caatggcagc tgtggcacca gaccccatgg aaataatgat ttgcttgtag gcagcatctg
    31921 tacagtcacc tgcagcaaaa ataccaggga tatttgttga gccaaattta tcaacgacaa
    31981 tttcttgacg ttcattgagt tcaatgccgc tatccttgag ccaagaagta cttggtacaa
    32041 gaccaatttg tacaaaaaca ccttctaaat caatgtgttt ttcttcgttt gtatcacgat
    32101 cggtatagtt taaaccagtg acatgatctt caccaacgat atctttagta gcaacattct
    32161 taagaatagt taaattgtca gttttagctg ctcgttcttg cagtacttgg tctgctttga
    32221 gctcaggtaa gaattctagg actgtaacat gtttagtgac accggctaga tctaaagcag
    32281 cttccatacc tgaattacca ccacctataa cagcaacatt ttttccttcg aaaagagggc
    32341 catcacaatg tggacagtaa gtaacacctt tattgcggaa ttcttcctcg ccaggaacgt
    32401 tgatatttcg ccacttagca cccagagcta ggatagcagt tttagcttgt aaaatagctc
    32461 cgttagccag tgttacctca actaattctt ttttctcaat gccagttgct aattgactct
    32521 tgataatatc aatatcgtaa gatttggtat gctcttcaat ttgagccatt aatttagggc
    32581 cttcggtgta cagtgtacca atcatgttct caataccaac tgtttcgata acttgtccac
    32641 caaaagtttc tgctaaaata cctgttttta accctttacg agcggcataa attgcagcac
    32701 tgttacctgc aggaccacca ccaataacta gaacatcata gacacctttg tcagcaaatg
    32761 cttcagcatc aagaggtcca tctagttgtt ctaaaagctg ttcaattgta gcacggccag
    32821 aagtaaattc ttcttggtcc ttataaacag taggaacaga catgatacct tttgatttga
    32881 cttcgtcttg atacatgcct ccttcaatca tagtatgagt gatatttgga ttcaaaacag
    32941 ccattatatt aaaggcttga acgacatctg gacaattatg gcaagttaat gaaacataag
    33001 tttctagatt gatagttttc tcaatcccct taattcgttt gataatatct tcatcaactt
    33061 ttggtgcgcg accagagact tgcaacaaag ctaaaatgaa agatgtgaat tcatgcccca
    33121 ttggcaagcc tgaaaaaata acgcgacttt cttgcccttt ttttgcaatc ccaaaactag
    33181 gttgacgttt taaatgtgtt gattcaaggc tgatacgatc agacatagcg acaatttcat
    33241 ctagaaaatc ctttactttt tgagaattat cgttatctcc taaatcagtt tgtaatacaa
    33301 tatcagattc tagcaaatct aaatattgcg ctaattgagc cttgatttct ttatctaaga
    33361 ccatggatta cctcctggtt attaaatttt acctacaagg tctaaactag gagttaaagt
    33421 ttcagctcct tctttccatt tagctggaca aacttcacct ggatgttggc ggatgtattg
    33481 agcagcacgg actttatcaa tgagagtact tgcatcacgt ccgatgccat cagcattaat
    33541 ttccatcatt tggatgacac cgtctggatc aatgataaat gtaccacgtt gtgctagacc
    33601 atcttgtcca aggacatcaa aaccttgtga aataaggtgt gaagggtcac caatcatagg
    33661 atatgtgata gtaccaacga catctgagtc atcatgccat gctttatgaa caaaatgagt
    33721 atcagtagag acagaataaa cttctacatc aagagattta agtgtttcgt attgctcttg
    33781 aaggtcacca agttcagttg ggcaaacaaa tgaaaagtct gctgggtaga aacaaaaaac
    33841 tgcccatttt cctttaacgt cttcatttgt aacagtgatg aattttccat cgtgataagc
    33901 ttgagctgaa aattcaataa tttcttttcc gactaatgac ataataatgt cctcctttat
    33961 tttttaatag cctaattata aacgaaatac ttttaaatct ccaataaaaa cgtttacatt
    34021 tttctaagaa aagtatgatt tttctaatag agtggaagat tttatagctt gtcaaatcta
    34081 actttttttg ataaactact atagttgctt aaagtgacaa atactcatct tagatcaatt
    34141 gtggtcttgt tgccgaaagg ttaggctgca agtcgaagtc tgagagagga aaaaacattt
    34201 ttaaaggaga acttactcat ggcagtaatt tcaatgaaac aacttcttga ggctggtgtt
    34261 cactttggtc accaaactcg tcgctggaac cctaagatgg ctaagtacat cttcacagaa
    34321 cgtaatggta tccacgttat cgaccttcaa caaactgtaa aattagctga ccaagcttac
    34381 gaatttgttc gtgatgctgc agcaaacgat gcagttatct tgtttgttgg tactaagaaa
    34441 caagcagctg aggctgttgc tgaagaagct aaacgtgctg gtcaatactt cattaaccac
    34501 cgttggttag gtggaactct tactaactgg ggaactatcc aaaaacgtat tgctcgtttg
    34561 aaagaaatca aacgtatgga agaagaagga acttttgaac ttcttcctaa gaaagaagtt
    34621 gcattgttaa acaaacaacg tgcacgtctt gaaaaattct tgggtggtat cgaagatatg
    34681 ccacgcatcc cagatgtaat gtatgttgtt gacccacata aagagcaaat cgctgttaaa
    34741 gaagctaaaa aacttggtat tccagttgta gctatggttg acactaacgc tgatccagat
    34801 gatatcgatg ttatcatccc agcaaacgat gacgctattc gcgccgttaa attaattact
    34861 tctaaattag ctgatgcagt tattgaaggt cgtcaaggtg aagatgctga cgttgacttc
    34921 gctcaagaag cacaagcaga ttcaattgaa gaaatcgttg aagtagttga aggatcaaac
    34981 aacgactaat atttaattta ttgtaaatcc taaaacgggg cagatgcaaa ccgctctgtc
    35041 tcgttttttt aatcattatt tgaaattaag aggagaacga aaatggcaga aatcacagct
    35101 aaacttgtaa aagaattgcg tgaaaaatca ggtgcaggcg ttatggacgc taaaaaagca
    35161 ttagtagaaa ctgatggtga ccttgataaa gcgattgaat tacttcgcga aaaaggtatg
    35221 gctaaagcag ctaaaaaagc agaccgtgtt gctgctgaag gtttaacagg tgtttatgtt
    35281 gatggtaacg ttgcagcagt tattgaagtt aacgctgaga ctgactttgt tgctaaaaac
    35341 gaccaatttg taacattagt taatgaaact gctaaagtta ttgcagaagg acgtccttca
    35401 aacaatgaag aagcacttgc tttaacaatg ccttcaggtg aaactttaga acaagcattt
    35461 gtaactgcaa ctgctacaat tggtgaaaaa atttcattcc gtcgcttcgc tttagttgaa
    35521 aaaactgatg agcaacattt tggtgcatac caacataatg gtggacgtat cggagtaatt
    35581 actgttgttg aaggtggcga cgatgcactt gctaaacaag tatcaatgca cgttgcagct
    35641 atgaagccaa ctgttctttc atatactgaa cttgatgcac aatttgttca tgatgaatta
    35701 gcacagttga accacaaaat tgagcaagac aacgagagtc gtgctatggt taacaaacca
    35761 gctcttccat tccttaaata tggatcaaaa gctcaattga ctgatgaagt tattgcccaa
    35821 gcagaagaag acatcaaagc agaattagct gcagaaggta aaccagaaaa aatctgggat
    35881 aaaatcgttc caggtaaaat ggatcgtttc atgcttgaca acactaaagt tgaccaagag
    35941 tacacactcc ttgctcaagt ttacatcatg gatgatagca aaacagttga agcttatctt
    36001 gagtcagtaa atgctaaagc agttgctttc gtacgttttg aagttggaga aggtattgag
    36061 aaagcttcta acgactttga agcagaagtt gcagctacaa tggcagcagc acttgaaaaa
    36121 taattaacac tcgctttata ttataatttg gcagcaaaat gttgtcagta aatatatttt
    36181 aaaataagac acttggctta agtcaagtgt cttattttgc tatcgaattg gtggattggc
    36241 aaatattaaa aaggagagac agtcatctca ccttaacttt tctagaatag gttgaagccg
    36301 ataatggata atgttcccat gatgcatcca gctagtataa cacctagcat aacagcaata
    36361 acactatgtt taaattccca atctagaagg ctagctgcaa gtgtgccagt ccatgctcct
    36421 gttccgggta agggaatgcc tacaaacaat agcaaggcaa taaacaatcc tttttctcca
    36481 gctacttttt caagtttttg accaccgcta tgtccttttt taaggcacca tgtgaaaaac
    36541 ttaccagtat aaggtttatc tgctccccat tctagaactt tgcgagcaaa gaagaagatg
    36601 ataggtacag gtagcatgtt tccaacaaca ccgatagcta gcgcttccca caaaggtatt
    36661 ccattagcga ttgcaaatgg cacagctcct cgtagttcca ctaagggaat cattgaaatc
    36721 aaaaatgtaa taatgtaatt cattaaataa aacctttcta tggtattatt ccattttatc
    36781 ttatcttgct agtaatgaca actaaaatcc gcggtcattc tagttttact atccttagat
    36841 agatttcatt tccgtatctt ttaaaaattt gatataataa tagtcagtta tagtcaaaga
    36901 ggaaggagtg aatttgtggc cataaaaaat acttccgata atattgaaga atatataaaa
    36961 tcattattag aacaatcagg aattgctgaa ataaagcgtt ccaatttagc tgatactttt
    37021 caagttgttc ctagtcagat taattatgtc attaagacac ggtttactga aagccgtggt
    37081 tatgtggttg agagtaaacg aggtggtgga ggttacatcc gcattgctaa ggttcatttt
    37141 tctgaccaac accaattatt tggaaatatg ttatcaacga ttggcgagcg tatcagtgaa
    37201 caagtctttg atgatttgat tcaattatta ttcgatgaag agataattac agaacgtgaa
    37261 ggaaatttga ttctggcgac atcaggtgat gacgtcttag gagagcaagc ctctgttatt
    37321 agagctcgta tgctccgtaa attattacaa cgattagata gaaaaggatt ccatttatga
    37381 gtcattattc cataaaatta caagaagtat tcagattggc gcaatttcaa gcagcacgct
    37441 atgaaagtca ctatttagaa agctggcatc ttcttttagc aatggtttta gttcatgatt
    37501 cagtagctgg tttgacattt gctgaatatg agtctgaagt tgcaattgaa gagtacgaag
    37561 cagcaacaat tttagcttta ggtagagcac cgaaagaaga gattaccaat tatcaatttt
    37621 tagagcaatc accagctttg aaaaagatct taaagttagc agaaaatatt agtatcgtag
    37681 taggagctga agatgtgggt actgaacatg tcttgcttgc tatgttggtt aataaagatt
    37741 tattagccac acgtatctta gaattagttg gctttagagg tcaagacgat ggtgaatcag
    37801 ttcgtatggt tgatttacgt aaggctctag aacgtcatgc cggttttaca aaagatgata
    37861 tcaaagcaat ttatgaatta cgtaatccta aaaaagcaaa gtcgggtgct tctttttctg
    37921 atatgatgaa accaccaagt actgctggtg atttagctga tttcacacgt gatttatcac
    37981 agatggcggt tgatggggaa attgaacctg ttattgggcg tgataaagaa atttcacgta
    38041 tggtccaagt cttaagtcgt aaaacaaaga ataatcctgt cttagttgga gatgcgggtg
    38101 tcggtaaaac agctttggcg tacggtctag cacaacgtat tgctaatgga aatattcctt
    38161 atgagcttag agatatgcgt gttctagagt tagatatgat gagcgttgtt gctggaactc
    38221 gttttcgtgg tgattttgag gaacgtatga atcaaatcat tgcagatatt gaagaagacg
    38281 gtcatattat tcttttcatt gatgaactgc atactattat gggttctggc tctggtattg
    38341 atagcacact tgatgctgct aatattttaa aaccagcttt ggctcgaggc actttacgca
    38401 ctattggagc aacaactcaa gaggaatatc aaaagcatat tgaaaaagat gctgctttat
    38461 ctcgtcgctt tgcaaaagtt ttagtagaag aaccaaatct tgaagacgca tatgaaattc
    38521 tacttggatt gaagccagcg tatgaagcct ttcataatgt taccatttca gatgaagcag
    38581 ttatgactgc tgtaaaagtg gctcatcgtt atttgacaag taagaattta ccagactcag
    38641 ctatagactt acttgatgaa gcttctgcca ctgtgcaaat gatgattaag aaaaatgctc
    38701 catcgctttt aacagaagtt gaccaagcta ttttagatga tgatatgaaa tctgcttcta
    38761 aagctctaaa agcaagtcat aaggataaaa agcataagcc tattgccgta accgaagatc
    38821 atattatggc aactctcagc cgtctatctg gtattcctgt tgaaaaattg acacaagctg
    38881 atagtaaaaa atacctcaat ttagaaaaag aattgcataa acgtgttatc ggtcaagatg
    38941 atgcagttac tgccattagt cgtgctatcc gtcgcaatca atctggcatt cgtactggta
    39001 agcgtcctat tggttccttt atgttcttgg gaccaactgg tgttggtaag acagagttag
    39061 caaaagcctt ggcagaagtg ttgtttgatg atgaatcagc cctaattcgt tttgatatgt
    39121 cggaatatat ggaaaaattc gcagcttctc gcctgaatgg ggcacctcca ggttatgttg
    39181 gttatgatga aggaggagag ctgactgaaa aagtacgcaa taaaccatat tcagttttac
    39241 tttttgatga agttgaaaaa gctcatccag atatttttaa tgtattatta caagtgcttg
    39301 atgatggcgt tttaacagat agtcgaggac gaaaagttga cttttcaaat actataatca
    39361 ttatgacttc taatttagga gcaactgcat tacgtgatga taaaacagtt ggttttggag
    39421 ctaaggatat cagccatgat tatactgcta tgcaaaaacg tattatggag gagcttaaaa
    39481 aagcttatcg accagagttt atcaatcgta ttgatgagaa agttgtattc catagtctca
    39541 gtcaagataa tatgcgtgaa gttgtgaaaa ttatggttaa acctttaatt ttagcgctta
    39601 aagataaagg tatggatctg aaattccagc cttcagcctt gaaacacttg gcagaggatg
    39661 gctatgatat agaaatgggt gctcgtccac tgcgtagaac tattcaaacg caagtagagg
    39721 atcatttatc agaactacta ttggcaaatc aagtgaaaga ggggcaagta attaagattg
    39781 gcgtatccaa aggcaaattg aaatttgata ttgcaaaatc ataaaacatt ccagtaccga
    39841 tgggtactgg aattttaata taaaaagaaa atgtccaaaa tattttggac attttcttat
    39901 agatttatga aaagtaaaag gatctatatc taacaattgc acttgtagat ataggtacga
    39961 ctgaaagtcg ttgaaaaatt ttaggataaa tatattataa tatcctaatc ttttgctaac
    40021 ttaaaataaa tctaaagatt accttaagat ttcaatcgct tttgtaacca tacaatgtca
    40081 tgccattgtt caaatttaaa cccgattttt gggaaatgtg cgacttgatt aaatcctctt
    40141 tttttgtgga atgcaatgct tatatcattt ggtaaactga tacaagcgag tagattaaca
    40201 tacccttggt taactaattc tttctccaat gtatcatata gttggctacc aatttttttg
    40261 ctacgtacat ctttatcaag gtaaatagaa agctcgcaag tccagtcata ggaagctcta
    40321 gcgtagtaag ttgaagcata tgcataacct aatattttcc cattttcttc agcaactaaa
    40381 tatgggaaag atttgctgat atttttgata cgattagtga attccgatat actaggaata
    40441 tcgtattcaa acgttattgt agtgtcagta acgtagggag catagatagc aagtaattct
    40501 ttagcatctg agggtaggac aggtcttata atcatagata tcacctaatt atagtaaatc
    40561 aagtttttgc aattcggaat caatagtgtc aaggacgtgt tgaagatcct ctggattttt
    40621 gacgaaatca agtttgttac catcgatccg aattttaggt gaaacgtcgt aattttcata
    40681 ccattctgga tattcagagt ggacttgttt gtagtagtcg tataattctg ggttagaatc
    40741 tacttgctca aagctgcgtc ctcgtttgtc aatacgttct aacattttat caaatgaaac
    40801 atcaatgtag actagcaaat caggtctctt cttaggcata ccttctaatt cttccaacat
    40861 atttgcaaga agttctttat agatatctag ctctgtttta gtcacatttc cattcttaaa
    40921 atttagagtg aggaatagtt catcttcaaa aatagaacga tccaaaacat tgttgttagc
    40981 cttataggct tcttttatag attggaatcg tttatttaaa aagaatattt gtagtaaaaa
    41041 agcatatttt ttgggatctt gatagtaaag atctaaaact gggttattat caaccgcctc
    41101 atagaaaaca tctgtcccta ggtgttgtcc tagagcagct gcaagggagc ttttacctgc
    41161 tccaatggtt ccagctaaaa caatcaacat aattctcctt aattctgtta tactaaaata
    41221 atttgttata cttttgagtt ttcattacac aatgatttat gctaaatttg tacagagata
    41281 ggaaaaaaca gtcaaaggac tgtttttgat ttagcgtaaa cctgcaaaaa gttcttgaac
    41341 ttctgctagt gtttctgcac gtgaaacagc tcctctaatt tttgctgcgc ctgattttcc
    41401 acgtagataa tgaggtgcta accctcgaaa ttcacgaacg gcaatagttt caccttttaa
    41461 attaactagt cgagtgagat gatcctcggc aacatctaac atcttctcaa atggtaggtc
    41521 gggaaggatt tcgcctgttt caaagaaatg attaatttgt gtaaagatgt aaggattgct
    41581 acgagcacca cgtccaacca ttatagcgtc agctccaatc tcttcaatca taaatttggc
    41641 atcgtgaaca gtacgaatgt caccattggc aataaaagga atactagtaa cagctttagc
    41701 gaccttgcca agtgtttcat ggtcacacgt gccagtatac atttgttcac gtgttcgacc
    41761 gtgcatagca agtgcagata caccagcaga ttcggcagcg agtgcatttt ctacagcatt
    41821 agatgagtca gaccatcctg tacgcatttt tactgttaat ggaatgtcaa gtacagacgt
    41881 tacctcttta acaatatgat aaattttttc agggtcacgt aaccatttag ctccagcttc
    41941 gtttttaaca actttgttaa ctggacatcc catattgatg tctacgatat ctgctttcgt
    42001 attattttgg ataaagtcag ctgcacgttt taagccttct gcatcgccac caaatagttg
    42061 aattgacata ggatgttcat tttcatcgat atggagcata tgtaaggtct tttcgttgtt
    42121 ataaagtaac cctttctcag aaatcatttc cataacaacg agaccagcac caaattcttt
    42181 agcgatagta cgaaaagcag aattagtaat acctgccatc ggtgctagta cggtacgatg
    42241 aggaatttca accttcccaa tcataaatga actatttaat tgagtcatta attatcttct
    42301 ccaaatcttc ttctgtgaaa tagtaagtct ggttgcaaaa ttgacaagta atttcaacac
    42361 ctttatcttc atccttcatg gcttgcaatt ctttagttcc taaagtcaat agagcggctt
    42421 caaaacgttc ttttgaacag tcacaataga aggctaaaga gtcttccgat aagcgcttgt
    42481 agtcgtcttc tccgtaaata gctgaaagga gagattctat atgattttca gactctagca
    42541 gacttgaaat agaaggcatc tcctgaatgc gtttttcata gcgagaaatt tcttcgtcag
    42601 aagcacctgg tagcacctga agcatgaatc cacctgcaac tttaacctta tcctcgtcat
    42661 ctaagaggac atttaaaccg acggcagaag gtgtttgctc actttcggtt aaataataag
    42721 caaaatcttc tccaatttct cctgtaatta gtggagttgt tgaagtgtaa ggttgcccag
    42781 ttccgtaatc ggtaataacg acaaagtgtc cattccccat gaaaggtcca actaagacct
    42841 ctccagtagc tgttttcttg atatcaacac ccgtattttg gatgtaacct ttaacatttc
    42901 ctttagtatc agctacagaa ataatatgac caaaagagct atctccaata accttgacag
    42961 ttactttact atttcctttt tgatttgctg ctaaaatttg atttgcaatt aatgttctcc
    43021 caagagctac agtagaactt gaaagtgtct gatgtttttc ttgagctgtt cttacagttt
    43081 ctgtacaatc aaggacatag gcccggaaag aaccggaagt tgaaattgat tttataattt
    43141 tatccataag tcatattata gcataaaagg cttattttgc attggagata gtcaggaaaa
    43201 tatagatgaa aacggtataa attaatgata taaaaacgat actgtgtttt acagtatcgt
    43261 ttttatatag tggatatgat agtaagttaa gactcatcaa ctttttcttg ggtagattta
    43321 ccttcgttga gtaaggcttc aactattttt ttatcacgaa ttttgacaga atcattgatt
    43381 gtttcagccg atttaataac agcagattct aattgggcac gttccttacg tcctttgtct
    43441 atggcagcga taataccatt attttgagcc actaagcttt cagctaatgc agtgacagat
    43501 ttaatagaaa cagtggggct ttgtgcggtc ttctctaaca tcggaatcgc ttctttacta
    43561 gtttcagcca gcatttgcaa tgctgcatta ttagcgttga caatagcatc agcagtgaca
    43621 ccggatttga cagattgttg catcatgcct aactgagcga ttgagagttt cattgttgga
    43681 atggtatttc gacgtaacat accaagtttc tgacgcatat ctgacgatac tttgaccaag
    43741 tttcgcatct gtggtgttgt tgcccatgca acgtagagac ggctgacata ttccgtatgt
    43801 tgctgttcga gggtattgat aacttcagtc attcgggcta attggttact tttaatttga
    43861 tattcggacg tttggctatc taatgctaga atttcttgtt gtaagtggct tgcacgattg
    43921 gcagcctcgg cttgactcga ttcaataaaa gcaataactc caaccaaatt ttcaatagat
    43981 ttagtattat cttcaatgag catttcagca gagacgatat ttcttgccaa agtatcttct
    44041 tgtttgacaa catttgctgc catcatatcc attttttgct cgatgttttg tgagtcaaaa
    44101 taaaattcct gtagcgaggt cttgctttgt ttgaataatt tttgaatcaa gtttggtttt
    44161 ttctctaatt ctgccggagt agcatcttta tatttggcaa taaatccatt tagttcgcga
    44221 ttagcatttt ttagtaaatc atcaacttga ggaatttgaa tttttttctg ctcagacaag
    44281 atatgattaa cagtagtatt aacgccttct actgcggatt gtccaaaatc aaggagcgca
    44341 ttttggtcac cgacaaaagt atctaccaaa gctggtgttt tttcagagat agcagacttt
    44401 tgtgctggtg ttagtttttc aaaaaaggca atttgcccag tttggcttgt tgtctggttg
    44461 gaaataattt ctgttgtttt atctgtttta gtgatagcat tgtctgcaat ttggtcaata
    44521 tcaaaattaa aggtatcgct catttattgt tcctttcgtc ttgcatcatt cttaggctaa
    44581 cgtcaaaatc tttcatatca ctttcattca attggcgcag cgtttcatct aaatctaaat
    44641 taaatttttt aattgcttgt aatgcttgtt ctaggcgttc cttagcattg ttaaaatttt
    44701 taggagattg tttgattctg atgtagccag ccagaacatc tgtaaaacgt ttcatattgg
    44761 cttgatgaat tgctgctaat tcagctttgt tatctgcctc ttcaatcttt tttaagatag
    44821 catagtgatc atcttgaata ttgtgataga acggttttaa ttccggagca atgcgtatca
    44881 tttcatcttc ctgatcagaa ataggttcta gttctggaga ggcatgtagc tttattaatt
    44941 gttcgttaat ctcattacta aatgtatttg cttttttggt gatacgttta tagatattag
    45001 gctcaatggc tttttgtagt tggtttgctt cattacgaat taggtccaat tgaggtaata
    45061 tttgatgtgc taatttgata tattccttag aacgattgtc tttttttaaa atttccaagt
    45121 gtctaatgtc ttggtcagtc tctttaatat cggattttaa agcttcaata cgttggtttg
    45181 atgtaaaaca tttaagacgc tttttatcat aaacgtaata ggcacaataa ccaccaccaa
    45241 taattaacag agtagcaatc acctcactcc aaaagtctga aatcagggaa gcaagaacaa
    45301 caataccaag taatgttatt agattatgag aatttttgga atagcgtttt ttaaccataa
    45361 taatatttaa cctaactttc tgtagttgtt aatattttat cttaaatgaa ctagtgatga
    45421 caagggataa ctttatttta accaaaaatt ccttaagctt agcctaagga actcttttta
    45481 gttataattc tgggaacagt ttagccaaaa ttcgaggtgt aatagattga ccaggcccct
    45541 cttgacagta ggtgtgcata tacatcaatg ggttaaagtt tagttcaatg caagtagcat
    45601 ttttcttatc ttttgagtat gcttgagtgg cattaggaat aattaaatca actccacaaa
    45661 cccaagcccc catagcctct gccatctcag ctgctagttg tttatatgta ggatccattg
    45721 tatttgttac atctatagag tcaccgccag tggaaatatt agaattacgg cgaagttcta
    45781 tttttgttcc ttctggaggg atactgttaa cagtgtatcc ttgttgttcc aacattaact
    45841 gttctacttc tccgagctca ataacctcta gaggagagcg atgatcatag cctctgagag
    45901 ggttttggtt tttaagttta actagctgac taatagtatg aataccatct ccaacgacat
    45961 tagctgctac cctaagcaat acagcaatac agtccccctc aaggacgaag aaacggtatt
    46021 cggtaccctc aatatattct tccacaagaa tagcgctatc ttcggcaaaa gcaatgtcaa
    46081 ttgctttttc ataagaagct aagttagctg atgttttaaa aatagaaatg cctaaaccaa
    46141 agtttgtaga ttttggctta acgacgattg gtttatcttg gatttgtgaa aaatagttaa
    46201 gtgcctcttt acggtcagta aattcatctc caaaaggagt tgggaaatgc ttttcatcta
    46261 ggattttttt tgtaacaact ttgttagcca tagcgagcgg tacaatatag ttatcttttg
    46321 aggtcatatt accattttta acgtactcaa tatgactatt gtgccataat tttaggaatt
    46381 gatcttgttc atccagtact tcgaagttga ctcctttttg gataacgtca aatagtagca
    46441 attgggtaga aagttccatc gtttcatatc cctttaaggc gtagggagct tcccaagcat
    46501 aatcgtgata aatttgtcct tgtctttgtc caaatgtttc taaggaaagt ccttcaatca
    46561 tttctaaaag ttgaccagct accgttaatt taggtgactg aatttgcctt tttacagatt
    46621 ccaataggtc ttgatagtaa ggtggtaagt taaaatgttg gataacagat tgcatagcat
    46681 ccactaagtc agaaactggt gcttgattag gtaatttttc taatggatga ctgagtgcta
    46741 taaggtcatt taatctattg gcttccttga tatcttggtc aatatgacta ctagaatcta
    46801 tccacaaaag cgccaataag aaaaggtgaa cagtatcaac agtttcctgc gttataccga
    46861 ttgggctaaa tgggttgaga tcaaaagtgc gaaattctaa atatgtaatt cctttttcta
    46921 aataattacg acaggactta ctaccacgga gacgaacagg tgaataaaat tctttctcag
    46981 caatcaattg cccacttttt acagcatttt ctagatcgtt gacataatcc ttcaagctag
    47041 tataagaaat acgaatatct ttatgattca catagcctaa gtgactattt ctaagtgacc
    47101 ggacagggtt attaagtttc tgatttaaaa agtcttcttc agctactggg ctcgctccgt
    47161 atagataagt tagtaaccaa cggtaacgta aaaaattttg agacaatttc atgtaaagct
    47221 ggttctgaaa atcaatagca ttatctgctt gactcaattc aaagagagaa gttaataact
    47281 cttgacctaa accgagatta tagtgaattc cagagatgga ttgtattagt tttccataag
    47341 ttttctccaa atatttgcga taatcatatt caaaagcatc ctctaattgg gcaatctgta
    47401 tgtcttcttc cctcactttg ggtggcatag aaagcggcca taagtattca tcgtggttga
    47461 tagatcgtcc agcgacatca cttatagctt tcaaaaatcg aatggcttct tggctatcct
    47521 ttgcaatagg tgtaatgagt tctaattgag gttcactata atccgtttgg atataaggat
    47581 gatagttacg actccctaat gttttgggat ggggtgtttg agcaaccctt tgagtgggtt
    47641 ggtgaatacg aaggctttct ctctctaagc caaatgtagc ttgtagaatc ggtagatgag
    47701 agtggcttct ttgtaacagt tgatcgataa tcacgacaac attcctccta gtaacagatg
    47761 atactagttt atcaaaaaaa taataaaaac taagttttac ggcacagaaa actaaaataa
    47821 tcttatactg gtgtcataaa aaataaaaga ttgaactttt gagtccaatc ttattaatct
    47881 ataagttaaa aagagcttta gagttaatga gtttcaattt ttttaagata acgatagacg
    47941 cttggttcgg agatgttgag aacttcagcg acctttgaaa cggcaccttt taattggaat
    48001 acaccttttt cgtgaagttt agaaacaatt tctaccttaa cttcttgact taaatgaata
    48061 ttctgattta aaagactagg atcaacaatc tcactaataa tatcctgaat attgcctgat
    48121 agaacttcta caggttcctc ctgttgaggg agagaaattt tttgagggga cttagatagt
    48181 attttgttaa catttaaatt aactaagtct agaatatcca aagcaatatt ttggtatgca
    48241 gaaatatcaa ggttgataca taacatccct tcaagttcat tttgagcatt cttgatgaag
    48301 aaagtagatc cacgaacttc tttattgaga gggctgacaa tcgccttata gttagtaaca
    48361 aaatcttttt ccttataaac tttattcttg attaaatcaa gggcgaaagt cgttagaggt
    48421 gaacttatgg ttcgtccact aatgtgacta ttaaccaact caccaatata gatatcattt
    48481 tcatctacta catgcaaaac aatttcgtaa ttatcaccta aaacattatg gagaaacgtt
    48541 attatcgtct tccaatagtc taatttttct ttatccatat cgtagttcct ctatgctaat
    48601 cttactatta tagtaatata tttatggtta aaagacaata tcagactatt ttaaaagatt
    48661 aatgtaataa aaaattatcg taataaaaaa aagttaagaa aataaaaaat tattattgac
    48721 tatggttatg cttttaggta aaatgtgatt atattagcaa ctaaggagta atccatgaga
    48781 taagcctaat aattatgtcg gacttctttg ttgcacctta tccagttcgt agtacaatta
    48841 agtttcagta ttacctaaag gcgcctttat tgaaattgaa gcagtagtac acagacactg
    48901 agaataaaaa acttaactat ttttagcggg agtatattta tgaatatcat tattgggacc
    48961 agtcttttaa tacttgtatt agcgattttc actttattta attataaagc accctacgga
    49021 acaaaagcaa tgggagcatt ggcatctgcg gcttgtgcat ccttccttgt agaagctttt
    49081 caagattctt tctttggtaa agttttggga tttcaatttt taagtgaagt gggtggtgct
    49141 aatggttcac tttctggtgt agctgcagct atattggtta ctattgctat tggtgtaacc
    49201 ccaggatacg cagttttaat tggtttatct gtttctggaa caggtattat tcctggtttc
    49261 cttgcaggat atttagtggg atttcttgtt aagtggatgg aaagaaatat tcctggtggt
    49321 ttagatctta tttcaattat tattattggt gcaccattaa cacgtcttgt ggccaaactg
    49381 ttgacgccgc ttattaatag cactttgctc accattggag atatcttaac atcaggtgct
    49441 catagcaacc ctattttaat gggggttata cttggcggga caattgtagt agtggcgaca
    49501 gcaccacttt cttctatggc attgacagct atgctaggat taaccggaat gcctatggct
    49561 ataggagcct tgtctgtctt tggttcgtca tttatgaatg gtgtactttt ccataaatta
    49621 aaacttggaa gtcgtaaaga taatatagct tttgctgttg agcctctaac tcaagctgac
    49681 gtgacttcag ctaaccctat tccaatctat gtcactaatt ttgttggtgg tgcagcttgt
    49741 ggtattttaa ttgccttgat gaaattagtt aatgatactc ctggaacagc gacaccaatt
    49801 gcaggatttg ctgtcatgtt tgcctataac ccaatgataa aagtactaat aaccgctcta
    49861 ggttgtatta tcctatcttt actagcaggc tattttggag gcattgtttt taaagattat
    49921 aaattagtta caaaagaaga gctacaggct agagataact agttacttca tgaaaagcta
    49981 atagtaaata tcattaatat agatatgata tctgacttgt gataactgtt attgataatt
    50041 taaaattcaa cgactctaat aaaaagattg ttgaattttt ttattgttaa gaatgctggt
    50101 atcagtaaat ttcaagattt ctttatcaac tataatgtta gttttttacc gtttttttac
    50161 acttctaata attatttttt tctttttttc cgataatatt tgaggaattt ggcaaaaaaa
    50221 aacatttttt gctataataa ggtgtcagac aaactcgcag gtgaaattcc tgccatgttt
    50281 ttccagaaag gattggggag tcccaaaaag gctcactcag tgttaaataa atatgacttc
    50341 agttgttgta gtaggtaccc aatggggtga cgaaggtaaa ggtaaaatta cagatttttt
    50401 atctgctgac gcagaagtaa ttgcacgtta tcaaggtggt gataacgctg ggcatactat
    50461 cgttattgat aacaaaaaat ttaaacttca tttaattcct tcaggaattt tcttcaaaga
    50521 aaaaatttct gtcattggta atggtgttgt cgttaatccg aaatcattag ttaaagagtt
    50581 agcttatctt cacggagaag gtgtaacaac ggataacctt cgtatttcag atcgtgcgca
    50641 tgttatcttg ccataccaca tcaagcttga ccaactacaa gaagatgcta aaggtgataa
    50701 taaaattgga acaaccatca aaggaattgg gccagcttac atggacaagg ctgcgcgtgt
    50761 cggtatccgt attgctgacc ttcttgatag agaagttttt gctgagcgtt tgaaaattaa
    50821 tttagctgag aaaaatcgtt tatttgaaaa aatgtatgac agtacacctc ttgaatttga
    50881 tgatattttc gaagaatatt atgaatatgg tcaacaaatt aagcaatatg tgactgatac
    50941 atccgttatc ttaaatgatg ctcttgatgc cggtaaacgt gttctttttg aaggagcaca
    51001 aggagttatg ctcgacattg atcaaggaac atacccattt gtaacatctt ccaatccagt
    51061 agcaggtggt gtcacaattg gttcgggagt tggaccaagt aaaattaata aagtggtagg
    51121 tgtatgtaaa gcttacacta gccgtgttgg cgatggacca ttcccaacag aactttttga
    51181 tgaggttggt gaccgtattc gtgagattgg taaagagtat ggtacaacga ctggtcgtcc
    51241 tcgtcgcgtt ggatggtttg attctgttgt tatgcgtcac agccgtcgag tatcaggtat
    51301 tactaacctc tctctgaatt caattgatgt tctttcaggg cttgatacgg tgaaaatttg
    51361 tgtggcttat gaccttgatg ggaaacgtat tgactattac ccagcaagcc ttgaacagct
    51421 aaaacgttgt aaaccaatct atgaagaatt accgggctgg tctgaagata ttacagcttg
    51481 ccgtagctta gatgatcttc cagaaaatgc acgtaattac gttcgccgtg ttggcgaatt
    51541 ggttggtgtt cgtatttcta ctttctcagt agggcctggt cgtgaacaaa caaacattct
    51601 tgagtctgtt tggtcaaata tttaatatta aaaatctgac tgtacagtca gactttttga
    51661 tgtttacttt ttctttttaa tagatagata ctaataagac ttaaggactt cccagaaatc
    51721 aataaaacta ctataattga taaa
//
LOCUS       DATRNX010000003        44145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRNX010000003 DATRNX010000000
VERSION     DATRNX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..777)
                     /locus_tag="VBC81_000145"
     CDS             complement(<1..777)
                     /locus_tag="VBC81_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HEO6911217.1"
                     /translation="MWGLSFISEEDFYNHVQLTIDKYGKKLQSFDLKRFNKNIVDPIK
                     LIFDKSVYRSSWEQIISNEIFRQRDKSNNNDIGYFHQTIFQYIEHCRVPNNGEEGGWD
                     VIFENPNGITMPDGSQVSRVYVEMKNKHNTMNSASSGKTFMKMQNQLLQDDDCACFLV
                     EAIAKNSQNIQWEPKVDGRKMGHKYIRRVSLDQFYSLVTGQEDAFYNMCMVLPEVINK
                     AVSELDSSIIPNDTVFDEIRDISAQQNYDSEDLSIAMSFYL"
     gene            complement(778..1779)
                     /locus_tag="VBC81_000146"
     CDS             complement(778..1779)
                     /locus_tag="VBC81_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO6911218.1"
                     /translation="MKSIELFAGAGGLALGIEKAGFDTIGLVEFDSAASETLKYNRPN
                     WNVIHDDVANISKLDLEEYFLIKKGELDLLSGGAPCQSFSYAGKRLGLEDTRGTLFYH
                     YAVFLEKLQPKMFLFENVKGLTSHDKGKTYKTILNVFEEEGYTVQSKVLNAWDYGVAQ
                     KRERMIMVGIRNDLVNMTSFEYPKPHDYKPVLRDILLDCPPSQGMQYSEQKRKIFELV
                     PPGGYWKDIPESIAKEYMKSTWYMGGGRTGILRRLSLDEPSLTVLTSPTQKQTERCHP
                     LEVRPFTIRENARIQSFPDDWEFQGSVGNQYKQVGNAVPVNLAYEVGLKIKESLEVL"
     gene            complement(2074..2439)
                     /gene="rplL"
                     /locus_tag="VBC81_000147"
     CDS             complement(2074..2439)
                     /gene="rplL"
                     /locus_tag="VBC81_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6911219.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(2503..3003)
                     /locus_tag="VBC81_000148"
     CDS             complement(2503..3003)
                     /locus_tag="VBC81_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6911220.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(3033..3166)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3436..5544
                     /locus_tag="VBC81_000149"
     CDS             3436..5544
                     /locus_tag="VBC81_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6911221.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAEQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(5637..6581)
                     /gene="mmuM"
                     /locus_tag="VBC81_000150"
     CDS             complement(5637..6581)
                     /gene="mmuM"
                     /locus_tag="VBC81_000150"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6911222.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(6586..7962)
                     /locus_tag="VBC81_000151"
     CDS             complement(6586..7962)
                     /locus_tag="VBC81_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6911223.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(8092..8742)
                     /locus_tag="VBC81_000152"
     CDS             complement(8092..8742)
                     /locus_tag="VBC81_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6911224.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(8754..9403)
                     /locus_tag="VBC81_000153"
                     /pseudo
     CDS             complement(8754..9403)
                     /locus_tag="VBC81_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            9495..10043
                     /locus_tag="VBC81_000154"
     CDS             9495..10043
                     /locus_tag="VBC81_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6911225.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(10105..10701)
                     /locus_tag="VBC81_000155"
     CDS             complement(10105..10701)
                     /locus_tag="VBC81_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6911226.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(10712..11938)
                     /gene="clpX"
                     /locus_tag="VBC81_000156"
     CDS             complement(10712..11938)
                     /gene="clpX"
                     /locus_tag="VBC81_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6911227.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(12156..12650)
                     /locus_tag="VBC81_000157"
     CDS             complement(12156..12650)
                     /locus_tag="VBC81_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6911228.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(12730..13569)
                     /locus_tag="VBC81_000158"
     CDS             complement(12730..13569)
                     /locus_tag="VBC81_000158"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6911229.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            13618..14895
                     /locus_tag="VBC81_000159"
     CDS             13618..14895
                     /locus_tag="VBC81_000159"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6911230.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            14897..16180
                     /locus_tag="VBC81_000160"
     CDS             14897..16180
                     /locus_tag="VBC81_000160"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6911231.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            16272..16720
                     /locus_tag="VBC81_000161"
                     /pseudo
     CDS             16272..16720
                     /locus_tag="VBC81_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
     gene            16713..17369
                     /locus_tag="VBC81_000162"
     CDS             16713..17369
                     /locus_tag="VBC81_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6911232.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            17456..18370
                     /locus_tag="VBC81_000163"
     CDS             17456..18370
                     /locus_tag="VBC81_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6911233.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            18471..19325
                     /locus_tag="VBC81_000164"
     CDS             18471..19325
                     /locus_tag="VBC81_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6911234.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(19370..19588)
                     /locus_tag="VBC81_000165"
     CDS             complement(19370..19588)
                     /locus_tag="VBC81_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6911235.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(19656..20651)
                     /locus_tag="VBC81_000166"
     CDS             complement(19656..20651)
                     /locus_tag="VBC81_000166"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6911236.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(20648..21640)
                     /locus_tag="VBC81_000167"
     CDS             complement(20648..21640)
                     /locus_tag="VBC81_000167"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6911237.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(21633..22577)
                     /gene="mvaD"
                     /locus_tag="VBC81_000168"
     CDS             complement(21633..22577)
                     /gene="mvaD"
                     /locus_tag="VBC81_000168"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6911238.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(22559..23437)
                     /gene="mvk"
                     /locus_tag="VBC81_000169"
     CDS             complement(22559..23437)
                     /gene="mvk"
                     /locus_tag="VBC81_000169"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6911239.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(23553..24782)
                     /locus_tag="VBC81_000170"
     CDS             complement(23553..24782)
                     /locus_tag="VBC81_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6911240.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(24782..25468)
                     /locus_tag="VBC81_000171"
     CDS             complement(24782..25468)
                     /locus_tag="VBC81_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911241.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYIFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(25470..26096)
                     /locus_tag="VBC81_000172"
     CDS             complement(25470..26096)
                     /locus_tag="VBC81_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6911242.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(26204..27958)
                     /locus_tag="VBC81_000173"
     CDS             complement(26204..27958)
                     /locus_tag="VBC81_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911243.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(27948..29765)
                     /locus_tag="VBC81_000174"
     CDS             complement(27948..29765)
                     /locus_tag="VBC81_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911244.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(29812..30252)
                     /locus_tag="VBC81_000175"
     CDS             complement(29812..30252)
                     /locus_tag="VBC81_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6911245.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            30521..32593
                     /locus_tag="VBC81_000176"
     CDS             30521..32593
                     /locus_tag="VBC81_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6911246.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(32630..33040)
                     /locus_tag="VBC81_000177"
     CDS             complement(32630..33040)
                     /locus_tag="VBC81_000177"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6911247.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(33110..34459)
                     /gene="gdhA"
                     /locus_tag="VBC81_000178"
     CDS             complement(33110..34459)
                     /gene="gdhA"
                     /locus_tag="VBC81_000178"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6911248.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            34627..35136
                     /locus_tag="VBC81_000179"
     CDS             34627..35136
                     /locus_tag="VBC81_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6911249.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(35177..36946)
                     /locus_tag="VBC81_000180"
     CDS             complement(35177..36946)
                     /locus_tag="VBC81_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911250.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(36951..38690)
                     /locus_tag="VBC81_000181"
     CDS             complement(36951..38690)
                     /locus_tag="VBC81_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911251.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(38757..39230)
                     /locus_tag="VBC81_000182"
                     /pseudo
     CDS             complement(38757..39230)
                     /locus_tag="VBC81_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(39227..41095)
                     /locus_tag="VBC81_000183"
     CDS             complement(39227..41095)
                     /locus_tag="VBC81_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6911252.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(41106..42314)
                     /locus_tag="VBC81_000184"
     CDS             complement(41106..42314)
                     /locus_tag="VBC81_000184"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6911253.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(42499..43347)
                     /locus_tag="VBC81_000185"
     CDS             complement(42499..43347)
                     /locus_tag="VBC81_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6911254.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(43347..43727)
                     /locus_tag="VBC81_000186"
     CDS             complement(43347..43727)
                     /locus_tag="VBC81_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6911255.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(43799..>44145)
                     /locus_tag="VBC81_000187"
     CDS             complement(43799..>44145)
                     /locus_tag="VBC81_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911256.1"
                     /translation="TETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSLDHLSA
                     PLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLAALSNN
                     QEVIDRHPKKKF"
BASE COUNT        14311 a         8801 c         7065 g        13968 t
ORIGIN      
        1 taaatagaat gacattgcaa tagataaatc ttcagaatca tagttttgtt gagcactaat
       61 gtctctaatt tcatcaaata ctgtatcatt tggaataatt gaagaatcta attctgatac
      121 agccttatta atgacttctg gcaaaaccat gcacatatta tagaaggcat cttcctgtcc
      181 cgtcacaagt gaatagaatt ggtctaaact aacacgtctt atatatttat gtcccatttt
      241 tcgaccatca acttttggct cccattggat attttgagaa ttttttgcaa ttgcctcaac
      301 taaaaaacac gcacaatcat catcttgtaa tagctgattt tgcattttca taaaagtttt
      361 accagaagaa gcagaattca ttgtgttatg cttatttttc atttccacat agacacgact
      421 gacttgagaa ccatctggca ttgttatacc atttggattt tcaaaaatga catcccaacc
      481 accctcttct ccattattcg gaactcgaca gtgctctatg tattggaaaa tagtttgatg
      541 aaaatatcca atatcattat tgtttgattt atcacgttgt ctaaaaatct cgttacttat
      601 aatttgttcc cacgaacttc tataaacaga tttatcaaaa atcaatttta ttggatcaac
      661 aatattttta ttaaatcgtt tcaaatcaaa ggattgtaat tttttgccat acttgtctat
      721 agtcaattga acatgattat aaaaatcttc ttcactaata aatgataagc cccacattta
      781 taatacctcc aaactttctt ttatcttcaa tcctacttca taagctagat ttacaggaac
      841 agcatttcca acttgtttat attgatttcc tacacttcct tgaaattccc aatcatctgg
      901 aaaactttgt atgcgtgcat tttcacgaat agtgaacgga cgtacttcca gtgggtgaca
      961 gcgctctgtc tgcttttgag ttggagaggt gaggacagtc aaagagggtt catctaaact
     1021 caatcttctt aaaatacccg tacgaccacc acccatatac caagttgatt tcatatattc
     1081 ttttgcaatg ctctctggaa tgtctttcca gtatcctcct ggaggtacta gttcaaatat
     1141 ttttctcttc tgttctgaat attgcattcc ttgacttggt ggacaatcaa gtaaaatatc
     1201 ccttaatact ggcttataat catgaggttt tggatattca aaagacgtca tatttactaa
     1261 atcattacgg attccaacca taatcatccg ttctctcttc tgagcaacac cataatccca
     1321 tgcattcaga acttttgatt gcactgtata gccttcttcc tcaaagacat tcaaaatagt
     1381 cttgtaggtc ttacccttat cgtggctggt caaacctttt acgttttcaa aaagaaacat
     1441 tttaggttgc aacttttcca agaaaacagc atagtgataa aacaaagtac cacgagtatc
     1501 ttccaagcct aaccgtttcc ctgcataaga aaaagactga caaggagctc ctccactaag
     1561 taagtctaat tctccttttt taattaaaaa atattcttcc aaatcaagtt ttgaaatatt
     1621 agcaacatca tcatgaatca cattccaatt tggtcgatta tatttcaacg tttcagaagc
     1681 agcgctatca aattcgacta atccaattgt atcaaaaccc gctttttcaa tacctagtgc
     1741 cagacctcca gcacctgcaa ataattcaat tgacttcaaa tataactcct taattgattt
     1801 ttaatgcgca ttctataaca ataattacta gttattacag aaaataagtg cttgctttct
     1861 attataccac tatttcactc atagtataat gaaattcaat gataaagata accttgaatt
     1921 gaaatcttta aataccaaaa ataaaacccc accaagaata aaacttagtg gggttgtggt
     1981 ggggaactaa aatttttccc accattttat caccatgaaa tcgagtttaa accgtgattt
     2041 actaggcttc cacaatcaaa ttgcggtact cttttattta agagtaactg aagcacctgc
     2101 agcttcaagt ttttctttga tttcgttagc ttctgcttct gaagcacctt ctttgataac
     2161 tgatggtgcg ttatcaacga tagctttagc ttctttaaga ccttcgcctg tgatttcacg
     2221 aacaactttg ataacgccaa cttttttgtc tccagcagct gttaattcaa cgtcaaatga
     2281 gtctttagca gcagcagctt caccaccagc agcggcagca gctacaggag cagcagcagt
     2341 tacaccaaat tcttcttcga tagcttttac aagatcgtta agttcaagaa ttgtagcttc
     2401 tttaatttca gcaataatat tttcaatgtt taatgccatt gttatttcct ccaaaaatat
     2461 gttttaatat aaaattatgt agctaggcta cttgaagagt gcttatgcca cttcttcgtc
     2521 tttttctgca actgctttga cagcatatgc aatgttgcgt actggcgctt gaagtactga
     2581 aaggagcatt gaaagcatac cttctttgtt tggtaattta gcaagagcgt taatttcttc
     2641 aactgaagtg aatttaccgt ctactgaacc acctttgatt tcaagagctt ctgcatcttt
     2701 agcgaaatcg ctaataactt tagctggtgc aataacatct tcgttagaaa acgcaactgc
     2761 tgatggacca acaaaaagtt ctttaaggtc ttcaagacct gctttttcag ctgcacgagt
     2821 taaaattgag tttttgataa ctttaaactc aacatcgctt tcacgaagtg aacgacgtaa
     2881 gttagtatct tgctcaacag taagaccacg tgaatcaaca acaacgatac ttgctgctgc
     2941 tttcattttc tcagcaataa gttcaacttg ttcagctttt tttgcaataa ttgcttcact
     3001 cattagtttt acctccgttt ttattttttg ggataggcac aaaaaaatcg cacctaaacc
     3061 tagacacgaa agtacaatac gttattttat aatgacgttt tgtgcctcgg taggaatatt
     3121 atgagcactg ctcccctact gtcttaggtc agtttcatca gaaactttaa atagtataac
     3181 ttataattct agcattgtca ataattttta ttatttttct gaaataattt tatagtacca
     3241 aaaaatacta aaatagttat ctttatatca aaaaaataat attttagtat ttatttattg
     3301 acctttactg actaaaggtg tattatagta ccagaaagat aattaaatat ttaaattaga
     3361 cctctctata tgtattaaag tataggaatc aataattaac tttcaaaaat taaaatcaat
     3421 tgaggtattt aatatatggc aaataataat ttttataatc gtgacccttt tggaaatatg
     3481 gatgatattt ttaattctct aatgggaaat atggggggct ataacagtga gaataagcga
     3541 tacttaatca atggacggga agttactcct gaagaattca gtcaatatcg tcaaactgga
     3601 aaactccccg gtcaagagct aaataaccaa aacactccta caaaccaagt ctctgcagat
     3661 agtgttttga ctaaattagg aactaattta accgatcaag cacgtcagca tttgctagat
     3721 ccagttattg gacgaaacaa agaaattcag gaaactgcag aaattctagc tcgtcgtact
     3781 aaaaataatc ctgttctggt tggtgatgca ggtgttggta aaacagctgt tattgaaggt
     3841 ttggcacaag caattataaa tggtgatgtc cctgctgcta tcaaaaataa agaaatcatt
     3901 tcgatagata tttctagcct tgaagctggc acccaatata ggggtagttt tgaagaaaat
     3961 attcaaaata tcataaaaga agttaaggaa acaggaaata ttattctctt ctttgatgaa
     4021 atacaccaaa tattaggagc cggttcaact ggtggtgata gtggctctaa ggggctcgca
     4081 gatatactta agcctgcact ttcacgtggt gagttaacgg ttataggtgc aactactcaa
     4141 gatgaatatc gtaacaccat tctcaagaat gctgccttag cacgtcgctt taacgaagtt
     4201 aaagtcaatg caccttctgc acaagatact ttcaatatcc ttatggggat aagaaacctc
     4261 tatgagcaac atcataatgt tgttttacct gactctgttt tgaaagcagc tgtagattta
     4321 tctattcaat acatcccaca aagaagtctc cctgataaag caattgacct tattgatatg
     4381 actgctgcac acctagctgc gcaacatccc gtaactgacc ttaaatcctt agaaaaagaa
     4441 atcgctgagc aaagagacaa gcaagaaaaa gctgtcaata ctgaagattt tgaagaagct
     4501 cttaaggtca agacacgcat tgaagagtta caaaaccaga ttgataatca taccgaagga
     4561 caaaaagtca ctgcaactat caacgatatc gctatgtcaa ttgagcgtct aactggagtt
     4621 cctgtatcaa atatgggtgc tagtgatatt gaacgcttaa aagaacttgg caatcgtcta
     4681 aaaggtaaag tcattggtca aaatgatgct gttgaggcag ttgcgagagc tattcgccga
     4741 aatcgtgctg gtttcgatga tggtaatcgt ccgattggta gctttctatt tgttggacca
     4801 actggagttg gtaaaaccga gctagcaaag caattagctt ttgatatgtt cggatcaaag
     4861 gatgctatcg ttagacttga tatgtctgaa tataatgatc gtacagcagt ttctaagcta
     4921 attggagcga ctgctggtta cgttggttat gacgacaata gtaatacact aacagaacgt
     4981 atccgtcgaa atccttattc tattgttctt cttgatgaaa tcgaaaaagc tgacccgcaa
     5041 gttatcacgc ttctccttca agttctagat gatggccgtt taactgatgg tcaaggaaac
     5101 actattaact ttaaaaatac tgttatcatt gcaacctcaa atgcaggttt tggaaacgaa
     5161 gcatttacag gtgacagcga taaagacttg aaaattatgg aacgaatttc tccatatttc
     5221 cgtccagaat ttctaaatcg tttcaatggt gttattgaat tctctcacct aagcaaagat
     5281 gacttaagcg aaattgtaga tttgatgctt gatgaagtta accaaacaat tggcaaaaaa
     5341 ggaattgacc ttgtggtaga tgaaaatgtt aaatcacact taattgaact gggttatgac
     5401 gaagcaatgg gagtacgtcc attgcgccgt gtcatcgagc aagaaattcg agatcgcatc
     5461 acagactact atctcgatca tacagacgtt aaacacctaa aagctaattt gcaagatggc
     5521 caaatcgtca tttctgaaag ataaaaacaa aacccgtttg gaagaatcca agcgggtttt
     5581 ctagtggatt gaaaaaggaa atcaaactta ccctaacctt cttcaagata ctttacttat
     5641 tttaaatgtg cagagaggtc agcaatatca gctggtctag ttcggcaaca acctccgaca
     5701 acttgggcgc caagtttttg ccagtcactt gtattttcaa gtagggtgtg tgaatgatca
     5761 gggctagatt gccaagattg gcttgcccca tcataaacct ctccagagtt tggataggtc
     5821 acaagaggct tgttagtctg ctctgctatt gcttgcaaga aatcagcaac taacgatgga
     5881 cttgaacagt taatgcccag cgccacgacc tgtgggctaa catcaatagc tttcgccaag
     5941 tctgcaacag cactaccatc tgagatggtc ttaccatctt gagaggtgaa tgacatataa
     6001 gcctcaacct gagggaaatc ctcggcaagt agctcaataa gagcctcagc ctcctgagcg
     6061 tttggaatgg tttcaagtgc cagaatgtca acaccctcat ctaaaagaag ctcaatacga
     6121 tggcgatgga agtttttaag accttgctta tcaatatcgt agagtccagt gtactcagag
     6181 ccatccgcta aaaaggcagc gtagggacca acatccccag aaatcaaagg atagattctc
     6241 tctgattttt cttcttttgt caaacttttc caaacttgct cacgcgcagc ttttgccaac
     6301 tgaacagtca agcgaatcaa atcttctgcc tgactttcag agacaccaac ttgagctagt
     6361 ccttgtaaag tcgcctgata agttgatgtc gtcacaatat cagcgccagc acgaatataa
     6421 tcttcatgaa tggtctgaat agctgctgga tcctcaatca gatatttggc tgaccaaagt
     6481 ttcccagaaa catcacaacc tctactctca agctcggtcc ccaaggcacc atgtaggatg
     6541 agagtttttt tactctctaa aagttcttta aatcttccca taagatcacc atttcttgta
     6601 acgtagtttg tgataaatat agcagaaaat aataaatgga acaccgaagt aaagcccagc
     6661 acgttgtgag ctatcccaag caatccctac aatcgaaatc aagagcaaaa tgattgtgat
     6721 ataagggaga actggtgtaa aaggtgtttt gtaagacaaa tcttctagtt gattctcttt
     6781 caagaattcc ttgcggaaat tgatttgtgc gactggaatg gctagccata cgacaacaac
     6841 agcaaagccc gcgattgaaa ccaaggctag ataaactgtg tctgcagcgt aaatacttga
     6901 aaagagcgac agcactgctc ctgccattga caagagaaga gcacgcattg ggacaccgtg
     6961 tttattgatt ttcacaacag attttgacaa cataccttca ttggcaaggg accagagcat
     7021 acggcttgat gcgtagagac ctgagttacc agcagacagg atggctgtca agataacgaa
     7081 gttcatgata tccgccgtaa aagggattcc catcttgtca aagacatcaa cgaatggtgc
     7141 tgtggatacg cctgcctctt tcattggcaa tagcgaagct aggacaacaa ttgtcagtac
     7201 aaagaaaaca accaagcgac cgattgtcgt tttaatagcc cttggtacag cttctttggg
     7261 attatccgtt tcaccagccg caataccaat taactcagta ccagagaagg catagttaac
     7321 agccaacatg actgagacaa ctgcaacgat accatttgga aaggcaccat tggcagtcag
     7381 atgagtgaag agaatagcct tgtgctgacc ttcaaaggaa actagaccaa acatagcacc
     7441 taagcccaag ataataaaga taatgatagc aataacctta atacttgaga agaaagactc
     7501 tgcttctgca aaaaagcgta cgctaagagc atttaaacca aaaatcacaa gggcaaaaaa
     7561 ggaagcaaat gcccaagccg gcacatttgg gaaccagcgc tgcatcagca tggcagcacc
     7621 taaaaattca gtccccaagg cgaccgtcca acaaatccaa tatagccaag caacagtaaa
     7681 acctgttcca ggactgataa acttagtggc ataagtgtgg aatgaccccg tcaccggcat
     7741 ggcaaccgcc aattccccaa gtgatagcat gaccaaataa accacaacgg caccaatcaa
     7801 ataagacagc acagctccaa gcggaccagc ctgtgcaatg gtataacctg aactcaagaa
     7861 aagcccagtc ccgataacac cacctagcga taacataaag agatgacgac tggtcatctt
     7921 acgctggaat ttaccttcat cttcataacg atgattttcc ataacaacct ccaagaaact
     7981 aatactttta tcctaccaca atctagtcgt attggggaat aggtattagc tatcatatat
     8041 gataaagaaa aactattaca aaaaatcagc gaatcataaa tagcggtaac cttatttttt
     8101 tatccttaca aaattaacga ccttatgaaa acggtccatt tgccaaggac taataatata
     8161 attggtctcc ttatccttca atagctgtac aggtgcaata atgctagtta gtacacagac
     8221 aatcaacatg ataaaaccac ctagccaatc tccccaagtg agcattcctt ctgcactagc
     8281 ttgatagatg tatgcgcaag ctattggtac taggagaaat actgtcgttg ctagaccagg
     8341 attataccaa gttttaagtt taatattcat ctgaaaacca tgacccaaga gctgcgttag
     8401 actaaagaac atgactccta atccaagcca ataagcttga ggaaaagcaa tactagcaat
     8461 gtaaagcaac caagcaatag tattaaccaa cataatagac tgagtattgc ctggaaaaca
     8521 atccatcagc tcttcttcat cataaacaac atagttaatg ataggtgatg ccccaccggg
     8581 aaactgatat tcttcaaatt gatgcgctag aagtgccata aagtaaaaat acaatagata
     8641 gtgaagcatt gaccagtcag gtctaaaaac aagcaatatt actgctaaaa taataaataa
     8701 aattgctcca aaacgatacc agtaatttct aaaatatgac atgataatac ctcctattta
     8761 aactgtgttt cacgaaaatg tgttaaaaat ggttttgagc gtgaaagatc aacatcacta
     8821 aatgcgtaat ttgaatgtcg acctaaacgc ttatagattg gggaacgaaa ggctataaag
     8881 tagttaagaa caatccatat cagcctaaaa accaatcaag tccagaatac aacttccaat
     8941 catgagctaa ataatagaca gaaacaggga caagacctac taaggtcgta agaagtcctg
     9001 gattatacca cttcttcaag gaaagattaa aaaagaaaag atgcatggca agttctgcaa
     9061 atgcaaaaat catgactgca agtgttaaaa atggtatatt gggacaaaaa atcggcaaaa
     9121 gataaacaat cactgcaaac cactgattcc cgaaaaaggc tgatagatgg tttaagtccc
     9181 acttactagt atcagtttca accgatttaa gttcaactag catagtgata agcggaaaac
     9241 ctccagggaa accaaattct tcgaaaaagt gtaattgaat aaaaagtccc gaaataagta
     9301 gtgactgcac tcgtaaatct caatttccaa aaaataatgc taaggcaaaa agagaagcaa
     9361 gatagggaca aaagaggtac cattttgtta tcaatgcttt cataagatga cctccaagat
     9421 ttgtgacatt tgataccata tagtataatc aagtgaacct ataatatcaa tacctttgga
     9481 ggttttatgc acaaatgact gaaagtgtga cagttaataa aaagagaata aaacaaatca
     9541 ctcaaacaca agaggatatc ttaacagctt ttggccaact tttggaagag tatccctatg
     9601 agaaaattca gattagccaa attgccaaga aatctggcta tgctagaagg accttttacc
     9661 gacactttga cagtcgagat gacctactta cccttttcat cgaaagacta acattaaatc
     9721 tctttaagca acttggtcaa ttggagcaac ctacttttag ccaagttttc caaaattttt
     9781 ttagttattg gtctgattat aaatcccttc ttctcattct tagaaagaat gacctactcc
     9841 ctcaatttca acaatcctgg ttccgacata ttgatttgat agagttaggt cgtggtgatt
     9901 tatcttccaa tacctatgct cagcgatttg cgattggtgg tattttcagt gttctaattg
     9961 agtggattca tcaggactgc caaacaagta tcgaagaatt aactcaagtt tccttcgata
    10021 ttatcaatca tttaaaaaat taaaaagagc tttgcagtag tctcaacagc tactgcaaag
    10081 ctctttttgt cataaactct caaattataa ctcagataaa atagtatccc atgattcatc
    10141 aagacctgtg cggtcaacag atgaaaagac gatgaaatgg tcctttttat caaagtttaa
    10201 tttcttctta atagacgact catgtttatt ccacttgcca cgaggtattt tatctgcctt
    10261 tgtcgctaca ataattactg gaatctcata gtacttaaga aattcataca tttgaatatc
    10321 gtccgcactt ggatcatgac gaaaatcaac taaactaaca acaacacgaa ggttatcacg
    10381 tgttacaagg tactcttcaa tcatcttgcc ccacttagca cgctcagttt tagacacctt
    10441 agcgtagcca tatccaggga catcgacaaa acgcagtttg tcatcaatgt tataaaagtt
    10501 gagcagttgt gtcttacctg gcttactcga cgtacgcgct aggtttttac gaccaagaag
    10561 cgtgttgata aagcttgatt taccaacatt tgaacgacca gcgagagcaa cttctgggag
    10621 atcatcctga ggataatgtg acttgttagc tgcactcaat aaaatagacg cattatgagt
    10681 atttaaaaat tcttctgcca tctctatctc cctaagccgt ctctaaaaca ggcttgtcag
    10741 taccctcaac agcagccttt gtgatacgaa cttttgttac atcttcttgg cttggaattt
    10801 caaacatgat atcaagcatc gtttcttcaa taatagaacg taaaccacgt gcaccagtct
    10861 tgcgctcgat agccttatca gcgatagcca atagagcatc ctggtcaaat tccaattcta
    10921 caccatcata agataataag gtttggtatt gtttaaccaa agcattgcgt ggttctgtca
    10981 gaatacgaac cagatcttct gcagtaagaa gttctaacgc tgcaactact ggtaaacggc
    11041 caataaactc tggaatcagt ccaaactttt gaatatcctc agaaattatc tcttgcatat
    11101 aagaagcgtt gtcatcaatt ttacggcttg tctgtccaaa accaataact ttttcgccta
    11161 aacgttgctt cacaaggtct tcaataccat caaaagcacc accgacaata aaaaggatgt
    11221 tcttggtatt aatttgaatc atttcttggt taggatgttt acgcccaccc tgtgggggaa
    11281 cacttgctac cgtaccctca ataattttaa gaagggcttg ctgtacacct tcaccagaca
    11341 catcacgtgt gatagaaaca ttttcgcctt tcttagcaat tttatctatt tcatcaacgt
    11401 agataatacc acgctcagca cgttcgacat tataatcagc agcttgaatc aatttaagaa
    11461 gaatattctc aacatcttct ccaacgtatc ctgcttcggt caatgaagtc gcatctgcaa
    11521 tagcaaacgg tacattaagg cttttagcca gtgtttgtgc taagaaggtt tttcctgagc
    11581 cagttggacc aatcatcaaa atgttggatt tttgcaaatc tacatcatcg tcactactct
    11641 cggtataact aacacgcttg taatgattgt agacagcaac tgctaaagca cgtttagcac
    11701 gatcttgccc tacaacatat tgatttaata tttctaatag ttccttaggt tttggtactt
    11761 ctgctaaatc agccagtact tcctcagcta attcttcctt aataatttct tgtgataagg
    11821 ccacacattc attacaaatg aaaacaccat tacctgcaat aatttttttt acttcatctt
    11881 ggcttttgcc acaaaatgaa caatagacat tcatatcgtt attacgattc ccagccattt
    11941 tttcctcaac ttttctattc atttaattta aaataatgtt ccataaaagg catggattgt
    12001 gttgacaagg ttagtgaaag cattcatcaa aaaaactgtt cctaatccat atcttttttt
    12061 ctttatagca ggaattgcac ataaaacaca aatcacagct aaaaatgcgg taaagacccc
    12121 aaaaaggcta cgttgcattg aatagtattc cttccttatt tttcaaaata tgtcacagta
    12181 aaagcatgag cattctgctc atccttttca aaataatctc tagagataac tttaaactca
    12241 gatagattaa catcaggaaa ataggtatca cctttgaatt ttccatgaac tttagttttt
    12301 atgattgctt cacaataagg cagaaatgct ttataaatac ttgcaccacc tacaataaat
    12361 aaggtcttat tatgttcctg aaaccatttt ataacttgtt caacactatt taggactgtc
    12421 actccatctg cttggaattg ttcatctttt gttaagatga ttgtctctct accaggtaaa
    12481 acacgacggt tcattccatc aaaggtcttt cgtcccataa gcaaagcctg ccccatggtc
    12541 gtttctttga agtgatgtaa ctctttagga agcctccatg gtaaaccgcc attaactcca
    12601 atcaaatggt cttcatcttc agcccaaatg gcaattattt gctttgtcat gacttttttc
    12661 ctcactaact tatctctaga tattctaaca aattttacct caaattgctt gttaaaaaac
    12721 atgagatagt tagattgcta aatcaaaacg taattgaggt ttaattgggt cataatcaac
    12781 taacgcaaaa tcatctggtt taatatcaaa aaagtctgtt ccatctggaa cattcaatac
    12841 tagtttgggg ttacactcag acggttggcg ttttaagagt tcctgagctt gttcaaactg
    12901 gttatcataa atatgaagat tattgataaa atagaaaaac ttcccaactc tccaaccaaa
    12961 atgctttgca atcatcatct gaagtgctac atactgcata gcattaatat gatgtgcaac
    13021 taacatgtca ttagaacgtt gtgttaatgt ggcatccaaa taaagctcac cattaacgcg
    13081 tctcacatca aacatcgttt gaaaagcaca aggtagtagg ccttctgttt cttcaaatgc
    13141 ttcatagtcc cataatgaaa taacattacg acgattccaa gggttatctt caagctgttt
    13201 taagagtttt gatataatat tatgtttttt aacaactgca ccgtagcgct gacctatcgt
    13261 gccagtatgg ccaacttccc aatcattcca gtatgtcaca ccatatttat cattcaaaac
    13321 tgctaaatca tttgtctgat cttgataaat ccagaaaatt tctttaatag cagattttat
    13381 tggaattgga cgtagagttg taataggaaa ttcacccttt gataaatcat actctgcaaa
    13441 ggcacctgta atatacttag aattagccat ttccccattt ttatatctag gtctggcttg
    13501 ctcgctaaat acaccttcag acataatttt cgtgatattg tctttaaata ataaatcggc
    13561 ttttgtcatg agatacctcc aaagtcttat tttatcagat tttaaaaaga tattctagtg
    13621 agaaacaaga ctttgaaggt taaagctttt tttcatattt ttagcttctt tatggtaaaa
    13681 tggaatttag caaaaatgac ttttattcag aaaggtttac caatgaaaat aggaattgat
    13741 aagattggtt ttgcaaccag tcaatatgtc cttgaaatga ctgatttggc tattgctcgc
    13801 caagttgacc ctgaaaaatt cagcaaggga ttattgttag attcacttag tattacgcca
    13861 gttacagaag atatcgttac acttgcagct tctgcagcta atgatatttt atctgatgag
    13921 gacaaagaaa ctattgatat ggtgattgtc gctacagaat caagtatcga tcagagtaag
    13981 gcggcttctg tctatgtaca tcaactttta gagattcaac ctttcgcaag gtcattcgag
    14041 atgaaagaag catgctatag cgcaactgct gctcttgatt acgctaaatt acatgtagaa
    14101 aaacatccag attctaaagt attagtgata gcttcagata ttgctaaata tggcattaaa
    14161 tctactggag aatccactca aggtgcaggc agtatcgcta tgcttattag tcaaaatcca
    14221 tccattctag agctaaaaga ggaccatcta gcgcaaaccc gtgatattat ggacttttgg
    14281 cgaccaaatt attctgatgt tccttatgtt aatggcatgt tttcaacaaa acaatatcta
    14341 gatatgctaa aaactacttg gaaagtatat caaaaacgtt ttaatactag tctaagtgac
    14401 tatgctgctt tttgtttcca tattcctttt ccaaaattag ctttaaaggg ctttaataaa
    14461 attctagata ataacttaga tgaacaaaag aaagctgaac tacaagaaaa ttttgagcac
    14521 tctattacct atagtaagaa aattggaaat tgctatactg gctcattgta tctaggactt
    14581 ctatcgttac tagaaaatag tcaaaattta aaagcaggtg atcaaattgc cttcttttca
    14641 tacggtagcg gagctgttgc tgaaattttt accggtcagt tagtagacgg ctaccaaaat
    14701 aaacttcaga gcgatcgtat ggatcaactc aataaacgtc aaaaaattac agtaactgag
    14761 tatgagaaac tcttctttga aaaaacaatc ctagacgaaa atggaaatgc taacttcaac
    14821 acctaccgga ctggcacttt ttctctagac tctatttgcg aacatcaacg tatctacaag
    14881 aaaattaata attaatatga caaaaatttc ctggacagga ttttcaaaaa aatcccctga
    14941 agaacgtatt cattaccttg aagaacaaga ttttttagct gattcttccc tagaaatagt
    15001 gacgaatcag gacttactct ctttatcact agccaatcaa atggctgaaa atgtcatcgg
    15061 tcgaatagcc ttaccatttt cacttgtccc agatgttttg gttaacggta aagtctatca
    15121 agtcccatat gtcactgaag aaccgtcagt tgttgctgct gcaagttttg ctgctaaaat
    15181 tataaaacga tctggtggtt ttctgacaac cgtccataat cgtaaaatga ttggacaagt
    15241 agccctttat gatgtacaag atagtcaaca cactaaagaa tctatattaa atcaaaaaca
    15301 gcaattatta gaaatagcca acgctgctca tccatcaata gtcaagcgtg gaggcggtgc
    15361 ttgcgaccta actattgaga ttaaagaaga ctttttgatt gtttacctta tggttgatac
    15421 aaaagaagcc atgggtgcta atatggttaa caccatgatg gaagcattat cttcgccatt
    15481 agaagacatt tcaaaaggca aaagcttaat gtcaatttta tctaactatg ctaccgaatc
    15541 tttagtgact gctacatgcc gagttgattt aagatttcta agtcgccaaa aagaggaggc
    15601 catcaagtta gctcaaaaaa tgacgatggc aagtcaatta gctcaagtag acccttatcg
    15661 tgctagcaca cataataaag gtatttttaa tggcattgat gctatcgtct tagctactgg
    15721 aaatgattgg cgagctattg aagctggagc gcatacctat gctgtcaaag atgggcaata
    15781 tcgaggatta tctagatggt cttataaggt tgatgataat tgcctagaag gtactctgac
    15841 gctaccgatg cctgttgcaa caaaaggtgg atccattggt attaacccta gtgtccacct
    15901 agctcacgat ctactaggtc gtcctaatgc taaagagttg gcaagtatta ttctttcgat
    15961 tggactagct caaaattttg cggctcttaa ggcattggtt agcacaggaa ttcaagctgg
    16021 tcacatgaaa cttcaagcaa agtcgttggc actacttgct ggagctaaag aagaacaaat
    16081 ttcagaagtc gttaaacagc ttctagactc taaacacatg aatctagaga cagcacaaaa
    16141 aattgtcaat aaacttacta aaagtaaaaa aagaatttga gaaaaatcat ctcaaattct
    16201 ttttttactt gacttgaatt tccataagtg tataatctag ttataaagac tagattatat
    16261 ggagttttaa aatgaataaa aatcaatacc caaaatggga agaattacct gaactagatt
    16321 tgtatcttga ccaagtactt ctctatgtga atcaattaat taatcctaaa acaataacta
    16381 acgataaact actcaccgct tcgatgatca acaattatgt caaacataat tatatatcca
    16441 aacctataaa aaaaaataca atcgccgtca agtagcacga ttgattgtta ttacagcttt
    16501 taaacaaatt tttgctattc aagaaatcag tcaaacatta gaacttctga cagctgacaa
    16561 tcattctgaa gaagcatata acggatttgc tgcttgtatg aacaaagaag aggttcacga
    16621 tttaccacca gttgtcatta gtgcctgtca gacgttgaac ctttatcaag aaacacaaaa
    16681 attagtttta gaactaggag gaaaaaataa aaatgcttaa agaaatgtct atcaaattaa
    16741 gtcctcaact ttcctttggc gaagagcttg caaatagtat cactcacgct gtaggagctt
    16801 tgcttatgct aatcctatta ccaatcaccg ctgtctatag tcacaaccac ttcggattac
    16861 aagcagcact tggtacctca atattcgtca cttctttatt tttgatgttt ttatcatcaa
    16921 gtatttacca ctccatgact tataacagtc ttcaaaaata tgttctacgc atgattgatc
    16981 actctatgat ttatattgct attgcaggta gttatactcc tgtcgcattg tcacttattg
    17041 gcggctggtt aggatatctc atcatttttt tacaatgggg gattacatta tttggtatcc
    17101 tttataaaat cttcgctccc aaaatcaatg ataaatttag ccttgtactt tacctcatta
    17161 tgggatggct ggttatcttt attttccctg ctattatcac aaaaactgga ccagcttttt
    17221 ggggattact cttggctggt ggtatttgct atactatcgg tgcactcttt tatgctcgca
    17281 aacgtcctta tgatcacatg atttggcacc tttttattct attagcttct attttacaat
    17341 acattggaat tgtctacttc atgctataaa atttaactac attttattga ttattttttt
    17401 gctaaaccat gatataataa taatcatcta aatcttagag agaaggcgtg gacatatgaa
    17461 aagtgcttat atctttttta atccaaaatc tggtaaagat gaacaagcat tggcaaaaga
    17521 agtaaaatct tatttaatag aacatgattt tcaagacgat tatgttcgta ttattacacc
    17581 ttcatctgtt gaggaagctg tcgccttagc aaaaaaagca tctgaagacc atatcgactt
    17641 ggttattcct ttagggggag atggcactat taataaaatc tgtggtggtg tctacgctgg
    17701 tggtgcttat cctactatcg gactagtacc tgccgggacc gtcaataact tttcaaaagc
    17761 tttgaacatc ccgcaagaga ggaatctcgc tcttgaaaac cttctaaacg gtcatgtaaa
    17821 gtccgtagat atctgtaaag ttaatgacga ttacatgatt agcagtttaa cacttggtct
    17881 ccttgctgat attgctgcta atgtcacttc tgaaatgaag cgtaaattag gcccttttgc
    17941 tttcttaggt gatgcctacc gtatattaaa gcgtaatcga tcatattcta tcactcttgc
    18001 ttacgataat aatgtacggt ctttacgcac gcggttactc cttattacca tgaccaattc
    18061 gattgccggt atgccagcct tctctccaga agcaacaatt gatgatggac tttttagagt
    18121 ctacactatg gaacatattc acttcttcaa attattgttg catctaagac aatttcgtaa
    18181 gggagatttt agtcaagcaa aggagataaa acattttcat accaataatc tgactatttc
    18241 aacttttaaa cgtaaaaaat cagctatccc taaagttcgt attgatggcg atccaggtga
    18301 ccaattacct gtaaaagttg aagtcatccc taaagcctta aagtttatca ttcctaactc
    18361 attaccataa tataaaagga cctagacata agtattagag taaatactat ctgtctaggt
    18421 tcttttttgt tatacttaaa gcaataaata tcattgaaag gaagtatacg atgacaacat
    18481 ttactgctaa atttattgac caagagtggg aagtcccagt agaatcaggt cgctaccata
    18541 tgattgttgg ggaattttgt ccttatgcac aacgtccgca gattgcgcgc caactgcttg
    18601 gtttggataa acatatttct atcagttttg tggatgatgt tccaagtgat ataggactta
    18661 tttttagcca accagaacag gtcactggtg ctaagtcgct tcgtgatatt tatcatttga
    18721 ctgacccgac ctaccaaggt ccttatacta tcccaatttt aattgacaag accgataatc
    18781 gtattgtctg taaggaatct gcagatttgc ttcgactttt tactacagat ttctctgatt
    18841 tgcatcaaga ggatgctcct gtccttttca gccaagaaac tgcttcactt attgataatg
    18901 acattaaaga cattaataag aacttccaaa gcttaatgta taagttagct tttcttgata
    18961 aacaagcaga ttatgatact tatagtaagg aattcttcac ttttctagat caaaaagaac
    19021 acttactagg acaacgtcct ttcttgctcg gagataatct aagtgaggtt gatattcatt
    19081 tctttacacc attagtccga tgggatattg ctgggcgtga tcttctacta cttaatcaga
    19141 aagcgctaga agactaccca aatatttttt cttgggctaa aactttatac aatgacttta
    19201 atttaaaaac attaaccaat ccacaatcca taaaaaataa ctattactta ggcaaatttg
    19261 gaagagctgt tcgtcatcat accatcgtcc caacaggtcc aaatatggca aaatgggaaa
    19321 aataaagcta aaaagctaag caattcacag ccttagcttt ttagctatac tagtccaaag
    19381 tggaccaatc aaattcaata aaagtcttga agagatctcc ataagtacca ctgtaagcaa
    19441 tatctttatc tttataagtc attttcgtaa taggataatt gtcaggaaca gtaacacatc
    19501 ccattaaaac tcgaataaaa tcatttggat tttcaaaaac ttggcatcgt tcagtgtcgt
    19561 aagcatcttt ataatacagt ttaatcatct tattacctct gacgttttac aaggaaactt
    19621 tgacaggaag ctatatctac ttcacaaaaa taatatcatt tagcattggc ttccttcaat
    19681 tgaccataca atatataatt gacttgtcgt aaatcagtta tttttttaca attcagtgca
    19741 cacattatca ttcttaaatc ttctttccaa gaattgagaa ttgcaataac atcatctaca
    19801 ggataacgct caactaattc taaaactgtt ctagaaagtc caacagcttt agcacccaac
    19861 actaaacatt ttaccatatc taaaggatga cgaatgccac cagatgctag gatatccatt
    19921 ttgtccatca tagattgagc gtttattaag gattgagctg ttgtttgccc ccaggtattg
    19981 agatacgaac ggtcgcgtcc acgttgattt tcgatgtaag caaaactagt ccccccacgc
    20041 cccgaaatat ctacagttgt gatacctata tcgtaagcat cctttattga ttgtaaatcc
    20101 atcccaaaac caacctcttt taaaataagt gggcattgga tattgtcgac gtattctttt
    20161 aagtgagaac gccacatgtg aaattcacgc tctccttcag gcatcaaaag ttcttgcatc
    20221 acattaacat gaacttgtaa aaaaatgggg ttcatagcct taacggttga ctctgctgct
    20281 ggtacaggct tgtctaaccc tatatttgtc gccagtttta aatcaggata caagtctgtt
    20341 gttgggtagg aatcatcttc atcgtttttg agagctgccg aatatgaacc tgtcaccata
    20401 acaatccctg tagcttgagc aacctgtgcc aatttatgat tgactgcttt ccctttttca
    20461 ctaccgcctg tcatagcatt gatataaaaa ggaaattcaa agctttgacc agcaaaatgt
    20521 gttgataaat cgatatcgtt gacattatat tttggtaagg atgaatgtat taactcaata
    20581 tcgtcaaaac tattataatg cgactgatat ttaagagcat attttatgtg atcatctttt
    20641 cgatttgtca tgacagtgtc tccttactaa gtagttcaat accagcactc tcccaacgct
    20701 tgattagagt ttgattatca tttttattaa aggatatggc tataccacaa tcgcctcctc
    20761 cagatcctga agattttgtg actatatcta ggcctttgct tgcctcttta agcttttgaa
    20821 gtttatcggt gtaaatagat ggatgaagat taaataatag caagctaatt tcttctaaac
    20881 atctcttaat cgcttcttta tctccttctt taaaggctga aagtaactgg acaacttgat
    20941 actttgtatc atctaaaaaa cgctgcgtaa tagttgattt aactatattt atcatttctt
    21001 ttgaaatgga cggttgcatt gtccatccca ctaaaaaatc catctccaat aaagcttgta
    21061 ttttggtaat acgataaccc cattcagctt ccaatacttg ttccaaaggt tttgtttcaa
    21121 tcatttttga aacagcccta cgatcaaaag ctgaataaga aattaaatgc tcataagcaa
    21181 tacaagcaat atctcccatt gagccattat cacctctttt taagagaaca taggcagata
    21241 atttaaaaag caagtcgctt ggcatctcta tctcataaag cgcagccata gctttaatag
    21301 tcaaaacgac aacacttccg ctagagccta taccaaattt aagtccatag cgctctaatt
    21361 ttcccgtaat ttcgaggtga atcggtttta tatttttgcc aaaattaatc aaccattctt
    21421 ccataagcaa tatcgtttcc tgaatgagac tatattgctt atctggctgt aagcttgccg
    21481 tatagttaaa catatcagaa taaaggctgt agttatctgc aaaagtagct aatgcagtca
    21541 tataaatagg aacgttcttt aaaatagcta cttgcccagg atacagtatc gcgtattcac
    21601 ctgcaatata taatttgcca cctgtttgca ccttaaccat ctgctaattc ctttgtcgtt
    21661 gaaacaataa tacgataatc tttctccaag atagcagcta atgcctcaag gtcttgacgt
    21721 aagcaaagta ctttaacatt tggacccgca tccatcgtat aataacattc atgacctttt
    21781 tcgcgaagtt tcttcacaac atccatagct ttatatgtct cttctgtcaa atatgaaaag
    21841 gctggagttg ctgttttagt tgttgagtgc attgcaagtg cattacgctc tgtcagttga
    21901 cctacctttt gaaaatcatt attttttagg tatactaaca tatcttgata atcttgttca
    21961 gattgcctta cccattcatt aaaagtagtt gaagtttcgg tgcagatttt cataccttca
    22021 cggcttgaaa tcggctttct tttgtcattt aagaccagca taatcatagc taaatctaaa
    22081 ttagtgtgaa ctttatagat atcaccacta tctttatccc aagcagcaac aggtccaaaa
    22141 aaagagcgtg aagaggagcc tgaagcaaac ttggcttctt gagctagctg tgattggcta
    22201 aggttagtac caaaaaaatc attgcaggcc tttactaagg ctgataaacc acttgatgac
    22261 gatgataagc ctgcagctgt tggcatatta ttagttgttt caatctttac aaagccggac
    22321 ctattttgtc gaaagcgatc caagatagct gacattttgt catgttcatg atcattttga
    22381 agaacaccag agatgtaaaa ttcatctttc ttagcatcct tcccaagggc agtcaaacgt
    22441 gtttcagtgt acatgttttc aagtgttaga gaaatactac tggtagctgg aatcattttt
    22501 tcagcatctg cttttcccca atatttgata atagcaatgt tagcataaga cttaacactt
    22561 atactttttc cgtccatgtg ttgacagctc cttctctttc taattgctga cttaagcgct
    22621 cagcttctct tttttctttt acaagtgcga taatacaacc acctaatccg ccgccactca
    22681 ttttagcgcc tagagcacca caactaatag ctacctctac cagatgatca gcttctaaac
    22741 ttgagacacc caattgtttt aaactttgat gtgctttagt catcaagcta ccaagttgtt
    22801 tggaatcact tttatgaata gcatcctctg atgcctctgc taaataacct aattctttta
    22861 aaaagggtag tactgcctcg ccagatgatt tcaccttatc cactgcttct ttagtatgcc
    22921 catgaattcc tgtatcagca ataactaaat aagcattcaa atgcatgggt actgttgaaa
    22981 aaccaatatt gcggataaat ttaatagtat tttcactaag acaagttttt gcatctaatc
    23041 cactcggatt tgaatgtgct atcatttctg ctcgattaac aaggcattct aacaaatctg
    23101 cctccaaatc ttcatcaaaa taatcaaaaa ctgcacgaat agctgcaata gcaactgcag
    23161 ctgatgatcc cataccacgt ctttctggga cttgtgactc aatatgatat gcaattttag
    23221 aagaagtttt acctaaatag tccaaggcag caaaaatggc tgtcgacaaa ggatcagtca
    23281 tatctaatgc tattaattga ggagcctctt caataaggca agttacttca atatttttta
    23341 aaggaatagc aatggctgga taaccatata ctactgagtg ctcacccatc aagataatct
    23401 tgctatgagc ctttcctata ccaaactttt ctttcatctt tttctcttta caattctatt
    23461 accttactat tttaatataa atccttaaaa aaagcgactt taacgtgata ataacataaa
    23521 ttaaaggaac tacaattgca gtccctttaa tcttaaatgt aaatgacaaa ggtaatggta
    23581 tcatgtttat aattcacatc gatacttcct ttaaatagat gcaccaaact agttacgata
    23641 gaaagaccaa taccatatcc cgattttttg ttattatgtg actcatcctc tcgataaaat
    23701 cgttcaaaaa acttgctata gtctatgtct ttaccattct tataagtatt tgaaacttca
    23761 agcttagctc gtctcaaacg acttgaacgt gataacttaa ctgtcacagt ccccatagga
    23821 tcacaatact tattagcatt atcaaccaat aatgtaacca attcaaataa tgatttttct
    23881 tcagctttga catgaatacc tggttggata ctcattataa agtctttacc atctttaatg
    23941 attggacctt taaaatcttc tgcagcatct tttgtaatat gtgaaaagtc taagtcaacc
    24001 atacttatat caggctgttc ttcaaagcgg gcaagactga ccatgccatt aattagaccc
    24061 gttaaacgtt ggatttgatc atttgtagat ttagtccact ctgattcacc actcatcatt
    24121 tccaccaatt cattattagc ggagatgata gcaagtggag tttttagttc atgtcctgca
    24181 ttggtaataa aacgcctttg cttttcatag ttagcaacaa atggtaagat aactctccct
    24241 gataaaactg aaacaattac catgaagacg ataaaactta ccaaggacat ccatattgaa
    24301 agttgcaata atctttgatt atcaagatat tggttggttg tatctaaaaa taccactaaa
    24361 taatcatttt taccaacctt ttttagtaga taagaataag tactaaaatt ataggtgata
    24421 tgacctgaat gactattttg tgatacgata cgcttggcat aattttttat ttgcctatcg
    24481 cttacttcaa agatatttct agaatcaaaa ctagtcacgt taccttttgc atctgttcgc
    24541 gcactgaaat aacggaattg gaaaatagcg tccgttgata ggtcatcacc taattcttta
    24601 ctaaattcaa gtttattcgg tatacgtcca ttgttgtctg caaggatagt taacacacga
    24661 taaatttcat tcttcgtttg atagtgatta gcagagttga ggacaccgac tactgaaaat
    24721 aagacaacaa gaatagctag cgcagctatt ccgataaaac gaagtcgtaa atttctaaac
    24781 attagccacc atttatttca cttaatttat agctactacc ttcttgacca ataatctcca
    24841 agttagcacc aattgcctgc aatttttgtc tcaaataaga aacatagata aagatgtatc
    24901 cctcatctac atcttcttgg tccttatctg ctccccagac atgttcgaaa agttgttgtg
    24961 tagataattc tttatcatgg ttcaacatga aaaatgctaa cattttagct tctttacccg
    25021 ctagacgaat agtgttttca cattgcaact cttgttcacc gactgataag gttactcttc
    25081 ccaatgacaa tacattaggt gtgaaatctt ccaatcttcg ggacatagag cgaagccttg
    25141 ctaataattc ttttagagag aagggttttg ttaaataatc atctgcacca gcatctagac
    25201 ctgttactct atcatcaatt tctgccatgg ctgttagcat aataatgtga gatttatttc
    25261 ctgattgtct aatttctttg acagcctcaa cacctgtttt aacaggcatc atgacatcca
    25321 taaccatgac atcatatgca ttttgattag ctaaatcaat agcagtctga ccatcgtagg
    25381 cgacatcaac aacataacct tgatgactaa ttgccgtact aaggacacgt gacatctgtt
    25441 cttcatcttc tgcaattaat actctcattt tattccttct ttgttccact ctctatcaat
    25501 tgacgaatcg tttgcatcgt taaatcaaca gatgctattt catcagcaca acgttttaat
    25561 tctcttacaa ttcgttcggg attttcaata tcatgcttgt atttcatctg atgttctaaa
    25621 ctagcccaag aatcttgcgc aattgtacgc aactgaatct ccatgtaata tttaccttct
    25681 gaattaccca aacagtccgg atagggagta tcaatttcta aaatcacatg gtaagagcga
    25741 tacccatttg gcttaacatg ctggatatag tctttttcaa ctacaatacg acaatcatca
    25801 aatgtcttaa tacgttctac aatttgataa atgtcgttaa caaaaccaca aataatacgg
    25861 actccaatac tatctctaat aacttttagt gcagaatcta ctgttacagg taactgttta
    25921 cgacgacatt tctctatcat actatctgat gtcttaatac gaccaatgat atgttcgtac
    25981 aatttatagc ctgtttcaac cttagcttta tcattttcta attggatacg ctgactgaaa
    26041 tcttctaaaa ttaatggtag atatcgacca taatctccat aaatatttgt ttccataaga
    26101 aaccttccta ttctctctta agctccatta tatcatagaa acttaaggca aactaaaaag
    26161 gaatgagaat tttttcttcc catttccttc taaaatgttt atgttaagct gattcgaatt
    26221 gagaattata cagatcagca taaaaaccac tttgagacat caaaacatca tgattgcctt
    26281 gctcaatgat attcccatct ttcatgacca aaatcaagtc tgcgttttta atcgttgata
    26341 aacgatgtgc gataacaaat gatgttcttc cttccataag tttgtccata gctttttgaa
    26401 ttaactcttc tgtacgtgta tcaacagatg aagttgcttc atctaagata agtaagggag
    26461 aatttttcaa aagagctcga gcaatggtta ataattgctt ttgtccaact gacaatgtca
    26521 cagcgtcatc aaggacagta tcataccctt taggtagtgt cattataaaa tgatgtatac
    26581 cgactgcctt agcagcttct tgaacctcat catcactgat tgtcgtctgg ttaaaaatca
    26641 aattttcttt aatagttccc tcaaacaacc atgtatcttg aagaaccatc gagaaagcat
    26701 catgaacttg actacgtttc atgtcttgga tattgatgtc atctatggaa atactacctg
    26761 aattaacatc ataaaaacgc atcaataaat taacaattgt cgttttacct gcgccagttg
    26821 gtcctacaat agcaactttc tgaccaggct gtgcctccga agtaaagtca tgaataatag
    26881 tcttgttatc actataacca aacttgacat gttcaaagga cacatgcccc ttaaacttag
    26941 gaagatttaa cactttgtgt ttatcctctt gcatatttgt ttcatttaaa aattcaaaaa
    27001 cacgtcccat ggctgcacta gcagactgta attgtgtaat tccttgagca atctgaccaa
    27061 taggctgtgt aaaaatacga acataggtca taaaagcaac aattgtaccc atagtaattg
    27121 ttcctttgat agctaacatg gcaccaacca cacacaccat cacataacca aaattaccaa
    27181 taaactgcat cagaggcatc ataattccag agaaaaattg tgatttccac atggaactgt
    27241 gaagtttatc atttaatagt tgaaaatctg acttagcctg ctgtcttgca ttatgactag
    27301 taacaatgtt gtgtcctgag taaatttctt ctacatatcc agatatatta gccaaattca
    27361 cctgttgctt tttaaataaa ggctgactct tcaccataat gattgaagaa agagcgaatc
    27421 ctgaaaatac cgatataatc gccgttgttg ctaaatgcca atctgttttg aacatcataa
    27481 ttaatgaacc aaccaataac actgtagatg caaccatagt aactaaactt tggttaaaag
    27541 actgtgtcat caaatccaca tcatttgtaa cacgagataa agtatcacct tgagtatggc
    27601 tatcaaaata actcaaaggt attttattga ttttatcagc aatagcattg cgcatacctt
    27661 gagcaaattt ttgaataata gtcgtcacaa taaaacttgc tagataggat actaatgcac
    27721 ctaaaacata taatactgcc aattgccctg caatatgacc gattttggct aagtcaatat
    27781 taccagccaa gccttttacc ataagatccg tcatctcttt aagacggtct ggtcctataa
    27841 ctgttgtcaa gcttgataaa gttgctaaca caattgctaa cgcaaaaaga agtttgtaac
    27901 ctttgaggta cggagataaa ttagagaaaa aagacgtttt ttgtgattta gttttcattt
    27961 tccaattcct ccttagacaa ttgagaatag gctatctctt gatatacttc attatttgat
    28021 aacaattcct tatgtgttcc ttgcccaaca actttacctt gatctagcac taaaatgaga
    28081 tcagcatcca tgatagttga aatgcgttga gcgacaatta atttcgtcat agcagctgtt
    28141 ttttctttca gcacatggcg taaaatatga tctgttttat aatctaacgc tgaaaatgag
    28201 tcatcaaaaa tgagaatttc agggtgacgt gctaaagcac gtgcgattgc caagcgttgt
    28261 ttttgtcctc ctgaaaagtt actaccacct tgagcgactt ctgtatctaa tcctttttct
    28321 ttagcttcta taaattcttt agcttgcgct aattcgagtg cttgccaaat ttgttcatca
    28381 ctaagttcct ccctgccact ctctccaaaa atcatattag aacgtactgt cccactaaag
    28441 agaacagctt tctgtggaat atagccaatt ttattattca aatcattgtg agagtattcc
    28501 tttatatcaa tgccatcaag tttaattgac ccctctgtgg cgtcataaaa acgtggaact
    28561 aaattgacta aagtggattt tccagaacct gtagacccaa taaatgccac tgtctgacca
    28621 gtatctgctt tgaaagatac atgttcaata accgcttcag aatctttact ataatgaaat
    28681 gacacatctt gaaactcaat ttgacctttt ggtgcacttt ggatatgact attttcttta
    28741 aaagttactg ttggggctaa agttagtacc tcattaatac gtctagccga tactaaagca
    28801 cgaggcatga tgataaaaat agctaccatc atcataaatc ccataacaac ttgcatagca
    28861 taagatgaaa aaacaaccat gtttgaaaaa agatccactc gatcagtgat agctgattta
    28921 gcagccaata cagatacact tgttcctgta actttaggca cagaaatatc attaatcaaa
    28981 taagagccaa tccaataaat agctaaagtc aatccagatg aaaccactgt catcacagga
    29041 ttcataagcg acatcaatct ataaacaaaa aggttcaatt ttgttaaatc gtcattttca
    29101 cgtttgaact tatctgtttg atacttttca gcattgtagg cacgaataac tctaacacca
    29161 gagagtgcct ctctagtagt tgcattaagc gcatctgtaa aaccttggat ttgacgttgt
    29221 ctcggaaaag caagtaagag tagaacgcta agtaatatca taacgataat aaccgctact
    29281 ccaactgccc cagtccattg atcgctttta cccccaattt tagtcaaggc ccatattgcc
    29341 ataattggtc cacgggtaac aacctgcatt cccattacaa taaggatctg tagctgagtc
    29401 aagtcgttag ttgtgcgcgt taatagagag gggatagaaa atcgcttaat ttctgcatca
    29461 gaataatcca tcacatggtt aaaaatatct gaacgtaatc gactagtaaa actagctgct
    29521 gtcctagcac ctaaaaaacc taccattact gctactaaaa aactaccaaa tgaaaaagca
    29581 agcattttta gtccaggatc ccaaagagcc tctaactttg tacctttagt ttgcaacata
    29641 cgtgtgatat cagacatata ttctggagtc tttaggtcca tccaaaccgc tagacacact
    29701 aatatagtac tcgccacaat catcaaccat tctttaccat tgagtcgctt gaaaatatta
    29761 aacatcgcaa actcctttta ctttcatatt tatcctatta tcctaaaata gttaatcaaa
    29821 atcttccatc gccatatttt tagctaatgt tctaataacc tttttggtag tacgtatctc
    29881 ttctttagta atacctgcta acatggtact gtgcaatttt tctaagaaaa tttcaaattg
    29941 tgtagccttt tgtttaccta gatgagtcaa ataaaggtat ttcacacgct tatctgtctt
    30001 agatggaaca atcgcaataa atccattttt ttccatccgt tttaccaaat tagacgctac
    30061 agacttagaa atgtgtaaaa tttcttcagc atcttttata gacatatcct tgtcaggatg
    30121 tttatagaga tgcatcacta agtgtccttg tggacccgct aaatgttcaa catcatactc
    30181 ttttgcataa cgatctaaat acttttcaag ttgattaaca agtattcgtg ctttttgaag
    30241 aggattctcc ataataatac tcctttaata gttatcgtga gaactatttt aaaacaacct
    30301 ttattaattg tcaattaaaa gttaccgtga gaactatttt tacattaata tttctaaaaa
    30361 acacctatca ttcgtcaaat aaaaaatgat ttcgcttact ttttaatatt aaaaatatat
    30421 gttcgctttt tatattttta attataaatt tatattgatt tttaatgtaa atgatagtaa
    30481 aatacaaatg taatacttat ttaatttaca ggagatttaa atgaaaaaga aaattatttt
    30541 gaaaagtagt gtccttggtt tagtcgctgg gacttctatt atgttctcaa gcgtattcgc
    30601 tgaccaagtc ggcgtccaag ttataggcgt caatgacttt catggtgcac ttgacaatac
    30661 tggaacagca aatatgcctg acggaaaagt tactaatgct ggcactgctg ctcaattaga
    30721 tgcttatatg gatgatgctc aaaaagattt caaacaaact aaccctaatg gtgaaagcat
    30781 tagagttcaa gctggtgata tggttggagc aagtccagct aactcagggc ttcttcaaga
    30841 tgaaccaacc gttaaaacat ttaatgcaat gaatgttgag tatggcacat taggtaacca
    30901 tgaatttgat gaaggtttgg cagaatacaa tcgtatcgtt actggaaagg cccctgctcc
    30961 agattctaat ataaataata ttacgaaatc atacccacac gaagctgcaa aacaagaaat
    31021 tgtagtggca aacgttattg ataaagttaa caaacaaatc ccttacaatt ggaaacctta
    31081 cactattaaa aatattcctg taaataacaa aagtgtgaac gttggcttta tcggaatcgt
    31141 taccaaagac atcccaaacc ttgtcttacg taaaaattat gaacaatatg aatttttaga
    31201 tgaagctgaa acaatcgtta aatacgccaa agaattacaa gctaaaaatg tcaaggctat
    31261 tgtagtcctt gctcatgtac ctgcaacaag caaggatgat attgctgaag gtgaagcagc
    31321 agaaatgatg aaaaaagtca atcaactctt ccctgaaaat agcgtagata ttgtctttgc
    31381 tggacacaat catcaatata caaatggtct tgttggtaaa actcgtattg tacaagcgct
    31441 ctctcaagga aaagcctatg ctgatgtacg tggtgtccta gatactgata cacaagattt
    31501 cattgaaacc ccttcagcta aagtaattgc agttgctcct ggtaaaaaaa caggtagtgc
    31561 cgatattcaa gccattgttg accaagctaa tactatcgtt aaacaagtaa cagaagctaa
    31621 aattggtact gccgaggtaa gtggcatgat tacgcgttct gttgatcaag ataatgttag
    31681 tccggtaggc agcctcatca cagaggctca actagcaatt gctcgaaaaa gctggccaga
    31741 tatcgatttt gccatgacaa ataatggtgg cattcgtgct gacttactca tcaaaccaga
    31801 tggaacaatc acctggggag ctgcacaagc agttcaacct tttggtaata tcttacaagt
    31861 cgtcgaaatt actggtagag atctttataa agcactcaac gaacaatacg accaaaaaca
    31921 aaatttcttc cttcaaatag ctggtctgcg atacacttac acagataata aagagggcgg
    31981 ggaagaaaca ccatttaaag ttgtaaaagc ttataaatca aatggtgagg aaatcaatcc
    32041 tgatgcaaaa tacaaattag ttatcaatga ctttttattc ggtggtggtg atggctttgc
    32101 aagcttcaga aatgccaaac ttctaggagc cattaatccc gatacagagg tatttatggc
    32161 ctatatcact gatttagaaa aagctggtaa aaaagtgagc attccaaata ataaacctaa
    32221 aatctatgtc actatgaaga tggttaatga aactattaca caaaatgatg gtacatatag
    32281 cattattaag aaactttatt tagatcgaca aggaaatatt gtagcacaag agattgtatc
    32341 agacacttta aaccaaacaa aatcaaaatc tacaaaaatc aaccctgtaa ctacaattca
    32401 caaaaaacaa ttacaccaat ttacagctat taaccctatg agaaattatg gcaaaccatc
    32461 aaactccact actgtaaaat caaaacaatt accaaaaaca aactctgaat atggacaatc
    32521 attccttatg tctgtctttg gtgttggact tataggaatt gctttaaata caaagaaaaa
    32581 acatatgaaa taataaaaat aggtttggat aaaatatcca aacctatttt tatatcaata
    32641 tgccttccaa atgatctaac tcgtgttgac aaatttgagc tggaaaatct gtcaacttga
    32701 tttgctgttc tttccaattg atatcacgat aagaaatcgt aatgtgatga tatcgttgtg
    32761 tacttctgca acctacgaga gaaaggcaag attcttctgt ctcatatgga tccgattttg
    32821 caactaatac agggttaaac attactaaat ttgtaatacc cacattgatg ataatgacac
    32881 gcttcagact accaatcata ttggctgcca tacctacaca ataattttga ttagcatgca
    32941 aagtatcctg taaatctttt gctaagctca catctgctct actagccatt tgtgattttt
    33001 gttgtaaaaa gaaagtatct ctaacaattg gttttatcat ttattttaac ctaactctat
    33061 tatgataata ctagtatacc ataggttagc taaggactat gataagagat taaacaatac
    33121 cttgtgcaga catagcttga gcaactttta aaaagcctgc aatgttagca cctagaacca
    33181 ggttaccact atcgccaaat tcatcagccg ctttggaagc attcacaaaa atagttttca
    33241 taatacgttg caattcttga tcaacttctt caaaagtcca tgccctacga ctagaatttt
    33301 gtgccatttc aagcgctgaa acagctactc ctcctgcatt ggcagcttta gcaggaccaa
    33361 aagcaacccc tgccttttgg aaaactttaa tagcacccaa ggtagatggc atatttgctc
    33421 cttctgttac tgccaagaca ccattttcaa ctaataactt agcgtcttct tcgtctaatt
    33481 cgttttgagt agcacacggg aaagctaaat ctgctttaat agaccagata ctaccttgat
    33541 ctgctggtgt gaaattagct tttggatgtt tctccgtata tttaacaata cgtgctcttt
    33601 caacctcttt tatctgtttc aatgtttcaa ggtcaatacc gtctggatca taaacataac
    33661 ctgaagaatc tgacacagct acaacagttg cacccaactc ttgtaatttt tcagtagcat
    33721 aaatagccac gttaccagat cccgaaacca gtgcaacttt gccagttaaa tcttgaccac
    33781 gcgctgccag catttcttta gcaaaataga ctgccccata accagttgct tctgtacgag
    33841 ccaaagagcc accataagtc agtccttttc cggttaaaac cccattttga taaccattta
    33901 aacgcttata ttgaccataa agataaccaa tttcacgtcc acctactcca atatcgccag
    33961 ctggcacatc taaatctggt ccaatgtatt tttgaagttc ggtcataaaa ctttgtgtga
    34021 aacgcatcac ttcattatct gatttccctt ttggatcaaa gttcgaccct cctttgccac
    34081 caccaattgg tagacctgtc agtgaattct taaaaatttg ttcaaatcct aaaaatttga
    34141 caatagattg tgttactgta gggtgaaaac gtaaaccacc tttataagga ccaatagctg
    34201 atgaaaattg tacacgatat cctcggttaa cttgaacttg gcctttatca tctacccatg
    34261 gaactctaaa agaaatcacg cgctcaggct caaccaaacg ttctaaaaga ttttcttcaa
    34321 tgtactgagg gtatttatca aaaactggta ctagtgactc aaaaacctct tcaaccgctt
    34381 gtagaaattc ttcctcatgc tcattttgct tcttcacttt ctctaaaaca cttgctacat
    34441 atgtctttcc gcttgtcatt tattttcctc cgtattgttt taatatagtt agcattataa
    34501 attgtcagaa ttttcttgtc aactatttgc tatggtatca ttattaggag caattttaaa
    34561 ggtaattatt tgacttttac tgactttcgt tctataatga gacaaatatt tgattggagt
    34621 atctatatga aatggttccc acttgttgga ggtataattg ccttattagc tggcttctat
    34681 ctctttataa atcctgtaac ggttgttgca actctgggtt ggttcattgc tttatttatc
    34741 tttatttatg gcatttcaaa tttagtacgc tacttgcaag ctgattctta tcaccgtagc
    34801 ccttggtatc tggttcagag tatccttgct attatttttg gtatcatttt actatccagc
    34861 tcagcaatgt cactttcaaa tactgtcatg actattttag catactggct actggtatct
    34921 ggtattttac gacttttggg tggtttccaa atgcaaaaaa ttaatttccc aggtgccaaa
    34981 tcctataaac gttcggctta tattgcactc attttaggta ttattttact ggttcaacct
    35041 accttatcag cagccatcat tggaagattt gtagccttta tattaatagc aattggagct
    35101 tcggctattt acacttttac tcgtattaat tattaaaagg acatgtttcg tgtccttttt
    35161 ttatctattg atgacgtcat ttactatttg aaaattggct attatataat tcgtaataaa
    35221 atcctttatc tgctaggagt ttctggtgat taccttgttc gatgatttga ccatccttta
    35281 gaactaaaat gttgtctgcc tcttgaatcg ttgatagacg gtgtgcaatg acaaaactag
    35341 ttcttccttc catcaatttt ttcatagctt tttgaataag caattccaac cttgtatcaa
    35401 cagatgaagt cgcttcatct aaaattaaaa ttttaggatc tgctaataaa gcacgtgcaa
    35461 tagtcaacag ttgcttttgt cctaaggaga tattgcttga ttcttgattc atcaccatat
    35521 tgtagccacc aggtaacgtt cgaataaagt ggtcaacatt cgcagctttg gcagcagcaa
    35581 ctatatcctc atcactggct tcaagattac cgaatctaag attttctttt attgtacctt
    35641 catataacca agcatcttgc aacaccatgc caaattgttg ccggaaatct tggcgtgata
    35701 ggtgtctaat atcgtgacca tctacggtga tagcaccttc actaacatcg tagaatctca
    35761 tgagtaagtt tataagtgtt gtcttacctg cacctgttgg ccctacaatt gcaatcattt
    35821 gccctggttc tacatctaag ttaaagtcgc ggataagtgg cttatccgga gaatagtgaa
    35881 aatcgacacc atgaaagctg acttgaccgg ttaagtcatg gctcaatttt tcagtgactt
    35941 gattggcttc ctcttcttca tctaagactt caaaaatacg ttctaaggaa gattttgcac
    36001 tttgcaaaac acctgctaat tgtgtaatgg tttgaacggg ttgactaatt tgccaaacat
    36061 actgcacaaa tgcctgcatg ttaccaattg ttaaggtacc agcaataact tgtaaaccgc
    36121 caacaaatgc aatgataaga taaataaaat cagagatact attcaatact ggcatcataa
    36181 ttcctgaaat gaagctagct ttgaaaccta catgacgtag attatccgtt atatcacgaa
    36241 attcttgact agacgcctct tctctaccat ataattttat gatattgaaa ccagttaatt
    36301 tctcttgtac aaaaccattc agttctccca gaatctttgc ctgttcttta aagtaaggtt
    36361 gggatctttt tagtatcgct tgtgcactaa aataagttac aggaatacaa gcaatgataa
    36421 tcatggctaa tggtacattt agataaagta ccataacgac tactagaata atacttaaaa
    36481 aagcattgat aatctgtaaa aagctctgtt gtaaagcatt tgagaccgtt tcaacatcac
    36541 ttgtaaaacg tcccaacata tctccaaatt ggtgcttatc aaaatacgat acaggaactt
    36601 tattgatttt acgattcaaa tcatgacgga tatcacggat acttttttga acgacagtag
    36661 tcataaaata atttgagcca taagaaccta attcatagaa aacacctctg aaaaaataaa
    36721 taatcagaat accagcaata taaggaacgt tcaattctgc cccagaaact cccttagcca
    36781 tatcaacaag gttagcagtc aactctgtta tcgctaaccc taaaataaaa ggctccagaa
    36841 tactcataaa actagataaa actttcaaaa aaatcgctaa gaaaagagta gctttgtagc
    36901 gagttaggta agaccataat ctcaaaaaaa tagatttttt tctcatacaa tcactctcct
    36961 tctgataagt tttggttctt cagttgtgac tcagcgattt cacgataaat agcattattt
    37021 tctattaatt gagcgtgggt accacgaccg acaatttcgc cttcatcaag gacaataatc
    37081 tgatcagcat ccataatcgt acccaccctt tgagcaacta tcaaaaccgt agaatcaccg
    37141 gttacttctt ttagacgcgc tcttaaagta gcgtctgtct tataatcgag agcagaaaat
    37201 gaatcatcaa aaatatataa atctggatct ttaacaacag ccctagcaat ggataaccgt
    37261 tgtttttgac ccccagaaag attgctccca ccttcagcta aatgcgtttc aaaggcttct
    37321 tggtgactct caataaactc tttagcttga gaaatatcaa ccgcttgtct aagatcatca
    37381 atagtagcat cagcttttcc atattttaaa ttctctccta ttgtccctgt aaataaaaga
    37441 gctttttggg ggataaatcc aatcttttgg cgaagtgatt taaggttata atctcttaca
    37501 tcaactccat ctactaagat ttttccaagt gtcacatcat aaaaacgtgg aatcaaatta
    37561 acaagagaag attttcctga acctgttgaa ccaataaaag caattgtttc tccaggctta
    37621 gctttaaaag aaatatcatg caaaacggga ctctctgttt ctcctggata agcgaatgtt
    37681 acattatcaa attctaaatg ccctttaagt ttcgtatccg taacaccttc ggtattagga
    37741 ttaatagaaa tcggcatatc cattacctct ctaatacgat ggctagatac caccatacga
    37801 ggatacatag taaaaagatt ggcaaatagc aaaaatgaaa agagagcatg gaagctatat
    37861 tcgataaaag caactaaatc ccctatttta atagcacctc tttgtaaggg atccaaagca
    37921 aaccaaacga tagccacaat cattgcaata ataatttgaa cgaaaagtgg ttctgttagc
    37981 cctgttaatt taaaaagacc agttgaagta tctgtgtaac gttggttagc gacttgaaat
    38041 ttttgtgatt gaaaattctc tcttgcaaag gccctaacaa cgcgtaaccc tgttaaattt
    38101 tcacgaacat attgattgat tttatcaagc atagtctgtt gtctttcaga taaaggtttt
    38161 gtttttatag ctacatataa aatgactcct accaaaagag gcatcgcaac cgctacaagc
    38221 caagccaaag atggactcgt aattagtatc ataaccacgc taaaaatcat taccatagga
    38281 gttactaggc ctaaacgtaa agacatttca gcaaattgca tcaaaacaaa agtatcgctt
    38341 gtcatacgtg tcactagtga agatacacct atctgttcat attcatgatg ggagtattct
    38401 tgaagcttag catacatatc attacgcata tctctaatca ttgttgtcgt taagcgacta
    38461 gatgcgtaag tcatcgtaat acgcccaata atacctagta caacaaatat aaacatgatg
    38521 aacgtccaca gataaactcc agtccgatca ccttttgtaa cgccattatc aatcataccc
    38581 gctaaggctg tgggcattcc taggataacc gtcacaaaaa gcatagctcc tagtaaatca
    38641 agccataacc aatttgggta ccttttcaaa taagaccaaa tatatgccat atatgtctcc
    38701 ttttctttcc tcttatctca aaataattaa tccatgttca ctaatccact ttcaacttat
    38761 aaatctttaa tcatccaaat atccatagct gtatgacctt gatcatttgc tattggttgc
    38821 gataaggcac aatatcccat atgcttataa accgcagttg ccctactcaa aatactggct
    38881 gtctctaaat aaagttgtct atacccaatt tttcgagctt ctacttcaat cattttcact
    38941 aaatcagtag caagcccctt accacggaaa cgttctgcaa tgtacacctt ctgcatctct
    39001 gcaattagat ttttcagcgg accaaagccg cttcagccaa taatctcatc tctctcctca
    39061 atgacaaaga atcctgactt ctctattttt tcgtagtatg aggtcaagtg atctaagtga
    39121 gggtctgaat atgctgtacc aggtttatct aaatcatagg cgcgtaaact ctgtcgaatt
    39181 aattgtgtaa ctgcttggtc atcattttta ataatcggac ggataatcat gtgtctaacc
    39241 cactaaggta ctcataacgg tcatactttt ctagaagtgc ttcatttttt gcatctaatt
    39301 ccttttgtaa atcagataac cttgtaaaat cactaccaca tgtttgcata tcatcttcta
    39361 tacgagtgat agtattttcc aatatcatca tatcgtcttc aattgtcgcc cattcttgtt
    39421 tttcaaagta agacattctt ttacgacttt gcttttcacg acttgtcttg gtactctctt
    39481 ttttactgat aacttcatta ttttgctcat taaatgcttt ttcatctaaa taatcagtat
    39541 aattaccaaa aaattcacgg atatcgttat cttcaaatgc aataatttta ttagccactt
    39601 tatctaaaaa gtaacgatcg tgactaactg taatcacagg accaccaaag ccttgtaaaa
    39661 aattttcaag aacagttaat gtagcaatat ctaagtcatt tgtcggctca tcaagtagta
    39721 acacgttagg cttttcaatc aggattttta aaaggtaaag tcgttttttc tcaccacctg
    39781 ataattttgc aatttgtgtt ccatgtgtcg aacgtggaaa gagaaattgt tccaatagtt
    39841 ctgtcacact tgttgtaccg acactagttt taacctcatc tgcaacctct tgcaaataat
    39901 taataacacg ttttgagcca tccatattat gaagttgttg tgaaaagtaa cctacacgta
    39961 tcgtttcacc aatagagaca ttacccgaat ccggctgtaa atcttgaaca attaaattaa
    40021 gtaaggttga ctttccaaca ccattatctc caacgatgcc aatacggtct ttattttgaa
    40081 ttaacaaatt aaagtctttc aagatagatt tatctgggta agaaaaagag acattttcaa
    40141 aattaataac ctttttccca attcgacttg tttcaaatgt catttccaaa tcgcttgtat
    40201 cgcttgtttg gtgtaaatcg ttttttagat tttgaaaacg attaatacga gcctgttgtt
    40261 tcgttgcacg agcttgtggc tgagtacgca tccaagctag ttcctgttta taaagctgtt
    40321 tctttttatg taaactagca gcatcacgct cgtcttgttc tgcacgaagt cggacataat
    40381 cctgataatt gccttgatat tctgtaatct gtgccttatc taattcaaaa atacgtgttg
    40441 caacattgtc tagaaaataa cgatcatgag ttataaaaag cactgtcttt ttactatttt
    40501 tcaaaaaatt cgttaaccat gcaatagtgt caatatctaa gtggttagta ggttcgtcta
    40561 agagcaataa atctgcatca tttaataata cttgcgctaa ttgaacacgt cttcgtaatc
    40621 ctcctgataa ttcaccaacc gacaactgca aatcagtaat acctaatttg gataatactg
    40681 ttttgacttc gctctcaata gaccaagcat ctaaagaatc catttctgcc attactttct
    40741 ctagacgtga ttgcttactt tcttcgtagt gattaagcaa taattcatat tctttaatta
    40801 aagccatctc tcttaagtca gaagaaagta cggtgtccaa aattgtctga gaatcatcaa
    40861 agtctggttc ttgttttaaa taagcaatct tataatcatt agctgatgaa aaaggggaac
    40921 gatcaccatc aaaacctaat tcacccgaaa taacatctaa tagtgttgtc tttccagttc
    40981 cattgacacc aataatccca atacggtcta aactatggat gataaatgaa acattactaa
    41041 agaccgtctt atcaccaacc gacttagtca atccatctac taaaaaatcg ctcatataat
    41101 cctctctaat ccatcaagta ctgcttaaca aaatgtaaaa tagcctcttc ttcattaatc
    41161 agttgtccta aaacaatggc taattcaatt tgagagataa tatctcccat ctgtggacca
    41221 ggttttattc ctaatttctt gattaaggtg ccaccattga caactatctc atgcctatca
    41281 tggatagcta actcactatc tagttgttct attttttcga aatcaaccat caagcctctt
    41341 gctttgcgaa taccttccgc ttgttttatt atagtttttc cataacgata aagatgttct
    41401 ttagtcaatc ccatttgttc acgaatacga taaacatcca ctatatgctc aacatctttt
    41461 tgaaagtgag ttgatgtctt ccattttttt aaaaaagtct ttgtgtgttc aacttccata
    41521 ctcaagatga gtgatgccca agcttgttct gatgtctcaa ataaaaaatc tttatctaac
    41581 tgggacaaaa aggcgcttaa ttgtaattcc ctatttttta agcatggtag ataattaaaa
    41641 gcatgactat ctattagtgc cagcattcct ttacgccaat aaggtgctaa tagaagttta
    41701 tcaaattcaa taaaagaacg ctcaacagat attttctcca ataatggcgc gtgctttttc
    41761 attgcctcaa aagttgttgt ttcgatatca aaatttagac tagcagaaaa acgcaaacct
    41821 cgcataattc gaagagcatc ttcattaaaa cgctcactgg cgagtcctac tgctctaaga
    41881 aggtgattat ctaaatcatc caatccatga aagagatcaa taacttctcc atcttcattt
    41941 aaagcaaagg cattaactgt gaaatctcga cgttttagat cttcttctag cgacctaaca
    42001 aaattgacct gactcggacg cctataatca acataaacct cctcagtcct aaaagtggta
    42061 atctcatatt caccaccttt ttccaatact aagactgttc catgttcaat accaacatca
    42121 actgtccttt taaaaatctg ctttgtttct tctggataag atgacgttgc tatatcaaca
    42181 tcatgaattg gacggtcaag taaaacatcc cgaacactac cacctacaaa gtatgcttca
    42241 taaccggctt tttttatctt ttttaatatt ggtaaagcct tctgaaactc agaaggcaaa
    42301 taatttaatc tcatcatact attccaaatc taaacgaact aaagtcgcat tattatttct
    42361 tataaatgga ttcagaaaca aaaagaactg cctaagggac actccctacc tactcaaatt
    42421 atcaaaaaag aatctcctaa cttcttcttt gatatctgca ataaggatca acctccaagc
    42481 aactcttttt atcacacatc aattttttgg ggtaatacca aaggcaaccg caccttcacc
    42541 aagatgagtt gcaataacgc caccaaatga tacaatttct aaatcgtctt taagaccagc
    42601 tttagctaac aaattatata attgttctgc cttatcttgt gctctagagt gaataatagc
    42661 aatatcatat tcaccatctg ctgtcatttc tttgacaatc tcagccaagc gttttaaggc
    42721 ttttttctct gtacgcactt tctcatagac aacgatttta ccttcttcat taaaatgtaa
    42781 gaccggcttg atacttaaaa ggttaccaat gatagctgaa ccgtttgaca atctaccacc
    42841 ttttactaga tgattcaaat cattaacaac aataaatcct tcaatttttt caatttgtga
    42901 ttgtatcttg ttaactatga catcaaaatc catcccttca cgtgaacaca tcaaagcatt
    42961 tctaactaaa ttgccctgtg gtgctgaagt tattttagta tccggaaaag caattgtcaa
    43021 atttggatgt tcttcaataa gaaactgaat attctgccag aaacctgata tccccgctgc
    43081 aatgaataaa ccaaggacgt gtgtataccc ttctttctcc aattgacata agagatcatc
    43141 tagttcagct aagctaggct gacttgtttt aggtaattct tttgatgccg ctagcttatc
    43201 ataatattgg tctaatgtca gattttgtcc ctcaatataa gttttaccat caatgataat
    43261 tggaatatct aaaacataga catcttcatg attttcgata gcctcaggca agtaagctga
    43321 ggtatccgta ataagtgcta atttcattaa ctattaaact ccaatgttac tcctggtcta
    43381 tctaaagcaa tttctgtaac ttcataagtc agacgtttaa cgatttctaa aagaggagct
    43441 gctagattct caacttcatc ttgactaaac tcactaggct cattgataag gcgcccttgg
    43501 atatgagcta tttgtgaaat gacaccagaa ataacaaact catcacgtac aataacaaat
    43561 tgtaacactg ctacaatgct cgttactttt gctgcttcat ctctattagc taattggaaa
    43621 gtaacttcaa aatttgtttt tggggtacca ttttcctctt cccattctaa attgcgcgca
    43681 tcatagtggt attgattgac aaattcttgt tcacgaatga cttccatgta ttttctcctt
    43741 gatagacaat atgtattatt atagcataaa tttgtagatt cttaaagcag gtgggaatct
    43801 agaacttctt cttaggatgt ctatcaatca cttcttgatt gttactcaag gctgctaaac
    43861 ctttttctgt aagtgccaac cctctgacaa caaacaactt ccctaattcc tcttctgtaa
    43921 aagcaatagc tattgcctga tctaaatcag agcgtttcat ctttgacaag ccttttacgt
    43981 ttttttcttt taagaatagt tttaaaagtg gagcagataa gtgatctaaa gaatcaagcg
    44041 gtgactccat tcttacaaat ccatttttta ttaaaaattc taggtgttct tttccattta
    44101 taccataaat gtattcaaaa tattttgaaa aacttgtttc agttg
//
LOCUS       DATRNX010000004        42402 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRNX010000004 DATRNX010000000
VERSION     DATRNX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42402
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..392)
                     /gene="dhaS"
                     /locus_tag="VBC81_000188"
     CDS             complement(<1..392)
                     /gene="dhaS"
                     /locus_tag="VBC81_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6911257.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYFESYCEQLVDKLLSDYSKSNFN"
     gene            566..1555
                     /gene="dhaK"
                     /locus_tag="VBC81_000189"
     CDS             566..1555
                     /gene="dhaK"
                     /locus_tag="VBC81_000189"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6911258.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1593..2171
                     /gene="dhaL"
                     /locus_tag="VBC81_000190"
     CDS             1593..2171
                     /gene="dhaL"
                     /locus_tag="VBC81_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6911259.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            2171..2545
                     /locus_tag="VBC81_000191"
     CDS             2171..2545
                     /locus_tag="VBC81_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6911260.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2555..3268
                     /locus_tag="VBC81_000192"
     CDS             2555..3268
                     /locus_tag="VBC81_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6911261.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3533..3937)
                     /locus_tag="VBC81_000193"
     CDS             complement(3533..3937)
                     /locus_tag="VBC81_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6911262.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(4076..4790)
                     /locus_tag="VBC81_000194"
                     /pseudo
     CDS             complement(4076..4790)
                     /locus_tag="VBC81_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4883..5992)
                     /locus_tag="VBC81_000195"
     CDS             complement(4883..5992)
                     /locus_tag="VBC81_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6911263.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5989..6240)
                     /locus_tag="VBC81_000196"
     CDS             complement(5989..6240)
                     /locus_tag="VBC81_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911264.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(6263..6997)
                     /locus_tag="VBC81_000197"
     CDS             complement(6263..6997)
                     /locus_tag="VBC81_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6911265.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(7063..7419)
                     /gene="rsfS"
                     /locus_tag="VBC81_000198"
     CDS             complement(7063..7419)
                     /gene="rsfS"
                     /locus_tag="VBC81_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6911266.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7421..7942)
                     /locus_tag="VBC81_000199"
     CDS             complement(7421..7942)
                     /locus_tag="VBC81_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6911267.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(8005..8592)
                     /gene="yqeK"
                     /locus_tag="VBC81_000200"
     CDS             complement(8005..8592)
                     /gene="yqeK"
                     /locus_tag="VBC81_000200"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6911268.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8589..9221)
                     /locus_tag="VBC81_000201"
     CDS             complement(8589..9221)
                     /locus_tag="VBC81_000201"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6911269.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9351..9668)
                     /gene="yhbY"
                     /locus_tag="VBC81_000202"
     CDS             complement(9351..9668)
                     /gene="yhbY"
                     /locus_tag="VBC81_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6911270.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIRVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9761..10879)
                     /gene="yqeH"
                     /locus_tag="VBC81_000203"
     CDS             complement(9761..10879)
                     /gene="yqeH"
                     /locus_tag="VBC81_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6911271.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10879..11439)
                     /locus_tag="VBC81_000204"
     CDS             complement(10879..11439)
                     /locus_tag="VBC81_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6911272.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11523..12437)
                     /locus_tag="VBC81_000205"
     CDS             complement(11523..12437)
                     /locus_tag="VBC81_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6911273.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12567..14009)
                     /gene="gatB"
                     /locus_tag="VBC81_000206"
     CDS             complement(12567..14009)
                     /gene="gatB"
                     /locus_tag="VBC81_000206"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6911274.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(14009..15475)
                     /gene="gatA"
                     /locus_tag="VBC81_000207"
     CDS             complement(14009..15475)
                     /gene="gatA"
                     /locus_tag="VBC81_000207"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6911275.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15475..15777)
                     /gene="gatC"
                     /locus_tag="VBC81_000208"
     CDS             complement(15475..15777)
                     /gene="gatC"
                     /locus_tag="VBC81_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6911276.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15915..18560)
                     /locus_tag="VBC81_000209"
     CDS             complement(15915..18560)
                     /locus_tag="VBC81_000209"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6911277.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18573..19403)
                     /locus_tag="VBC81_000210"
     CDS             complement(18573..19403)
                     /locus_tag="VBC81_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6911278.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19414..20064)
                     /locus_tag="VBC81_000211"
     CDS             complement(19414..20064)
                     /locus_tag="VBC81_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911279.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            20232..21197
                     /locus_tag="VBC81_000212"
     CDS             20232..21197
                     /locus_tag="VBC81_000212"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6911280.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(21244..21792)
                     /locus_tag="VBC81_000213"
     CDS             complement(21244..21792)
                     /locus_tag="VBC81_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6911281.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21859..22644)
                     /gene="codY"
                     /locus_tag="VBC81_000214"
     CDS             complement(21859..22644)
                     /gene="codY"
                     /locus_tag="VBC81_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6911282.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22770..23981)
                     /locus_tag="VBC81_000215"
     CDS             complement(22770..23981)
                     /locus_tag="VBC81_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6911283.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            24248..24700
                     /locus_tag="VBC81_000216"
     CDS             24248..24700
                     /locus_tag="VBC81_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6911284.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24755..26137)
                     /locus_tag="VBC81_000217"
     CDS             complement(24755..26137)
                     /locus_tag="VBC81_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6911285.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            26206..27168
                     /locus_tag="VBC81_000218"
     CDS             26206..27168
                     /locus_tag="VBC81_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6911286.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(27203..28081)
                     /gene="aroE"
                     /locus_tag="VBC81_000219"
     CDS             complement(27203..28081)
                     /gene="aroE"
                     /locus_tag="VBC81_000219"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6911287.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(28179..29093)
                     /locus_tag="VBC81_000220"
     CDS             complement(28179..29093)
                     /locus_tag="VBC81_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6911288.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(29384..31399)
                     /gene="recG"
                     /locus_tag="VBC81_000221"
     CDS             complement(29384..31399)
                     /gene="recG"
                     /locus_tag="VBC81_000221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6911289.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(31479..33017)
                     /locus_tag="VBC81_000222"
     CDS             complement(31479..33017)
                     /locus_tag="VBC81_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6911290.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPELTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(33110..34210)
                     /locus_tag="VBC81_000223"
     CDS             complement(33110..34210)
                     /locus_tag="VBC81_000223"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6911291.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVTSLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(34207..34566)
                     /locus_tag="VBC81_000224"
     CDS             complement(34207..34566)
                     /locus_tag="VBC81_000224"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6911292.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(34591..35598)
                     /locus_tag="VBC81_000225"
     CDS             complement(34591..35598)
                     /locus_tag="VBC81_000225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6911293.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(35724..38252)
                     /gene="secA"
                     /locus_tag="VBC81_000226"
     CDS             complement(35724..38252)
                     /gene="secA"
                     /locus_tag="VBC81_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6911294.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(38361..39308)
                     /gene="manA"
                     /locus_tag="VBC81_000227"
     CDS             complement(38361..39308)
                     /gene="manA"
                     /locus_tag="VBC81_000227"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6911295.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(39426..40307)
                     /locus_tag="VBC81_000228"
     CDS             complement(39426..40307)
                     /locus_tag="VBC81_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6911296.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(40375..42294)
                     /locus_tag="VBC81_000229"
     CDS             complement(40375..42294)
                     /locus_tag="VBC81_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6911297.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
BASE COUNT        13327 a         8536 c         6738 g        13801 t
ORIGIN      
        1 tgattaaagt tagatttact ataatcagaa agaagcttat ctactaactg ctcacagtaa
       61 gactcaaaat acgaagaaaa atcattttga tcttcaattt gaaataattt aatatagaat
      121 tcttgattca ttttaaaaaa agtcaacaac tctgataaca actgaaaacc tgagatatag
      181 tctaaattat ccgtaacttg ttcgcttaat tctgtttgga aaatccattc taataaagca
      241 tatttatcaa caaagtgatt ataaaatgtt tgacgtctaa tacctgcatc ttccataata
      301 tcactcaccg atattttgtc aaaagcttgc gaaataaata aacgtttaaa tgacttggct
      361 atttttttct tagtaataag cgaagaagtc ataatttctc ctttatttta tattgaattg
      421 ctgatgattt attatggttt agtgtaacat gatttatgat tattgaaaac cctaacaaag
      481 cggacaaaca agaaaatctg tccattccag aaaaggctta caaattttat aataaaagag
      541 aattaaacat aaaaggagtt tccaaatgaa aaaaattctc aatcaaccaa ctgatgttgt
      601 tactgaaatg ctagatggat tagcatatgt tcataatgat ttagtacatc gcatagaagg
      661 ttttgatatc attgctcgca atgaagagaa gtcaggaaaa gtggctctca tttctggcgg
      721 aggaagtgga catgagcctt cacatgctgg tttcgtaggc gaaggaatgt tatctgcagc
      781 tgtgtgtggt gcagtattta cttctccaac tcctgaccaa gttcttgaag caattaaaga
      841 agctgatgag ggggctggag ttttcatggt tatcaaaaat tattcagggg atatcatgaa
      901 ctttgaaatg gcacaagata tggctgaaat ggaaggcata gaagttgcaa gtgtggtcgt
      961 tgatgatgat attgcagtcg aagacagtct ttacacacaa ggaaaacgtg gtgtagctgg
     1021 tacgatactc gtccataaaa ttctaggaca tgctgctcgt catggcaaat cacttcagga
     1081 aatcaaagct attgctgatg aattagtacc aaacattcac actgttggac ttgcactaag
     1141 cggggctact gttcctgaag taggcaagcc aggatttgtt cttgcagaag atgaaattga
     1201 atttggtatc ggtattcatg gtgaaccagg ttatcgtaaa gaaaaaatgc aaccttcaaa
     1261 ggctttagcg acagaattag ttgataagtt aattgaatcg ttcgatgcta aatctggcga
     1321 aaaatatggt gtccttatta acggtatggg tgcaacccca cttatggaac aatatgtctt
     1381 tgctaacgat gttgccaaac tccttgagga taaaggcatt gaagtgaatt acaaaaaact
     1441 tggtaactat atgacttcaa ttgatatggc tggtttatca ctaaccttga ttaaactgga
     1501 aaatcaagag tggttagaag cacttaatag tgacgttaca accattgctt ggtaatcatt
     1561 cctgttaatt agaacaaaag gaaggaactc ttatggaagt aaaaactgca atagaatgga
     1621 tgcacacttt taaccaaaaa attcaatcca acaaggacta tttatccgaa ctagatactc
     1681 caataggtga tggcgaccat ggtggcaata tggcgcgtgg tatgactgct gttattgaaa
     1741 accttgataa caatgaattt agtagtgctg ccgatgtttt caaaaccgtg tccatgcaac
     1801 ttttaagcaa agtaggcgga gcatctggac cactctatgg ttctgcattt atgggaatta
     1861 caaaagctga acagagtgaa agtaccattt cagaggcact tggcgcagga cttgaaatga
     1921 ttcaaaaacg tggtaaagct gaacttaatg aaaaaacaat ggttgatgtt tggcacggtg
     1981 ttattgaagc gattgaaaaa aatgaattga ctgaagaccg tatcgattcc ttagtagatg
     2041 ctacaaaagg gatgaaagca acaaaaggac gcgcttctta tgttggagaa cgctcagtag
     2101 gacacattga tccaggatca ttttcttcag gattattatt taaagcatta ctagaagttg
     2161 gaggtgtcta atgtctgata ttggtattat tgttgtttct cactctaaaa atattgctca
     2221 aggagttgtt gatcttattt cagaggtagc aaaggatgtc tcaataactt acgtgggtgg
     2281 aacagaagat ggtgagattg gtacttcttt tgatcaagtt caacagattg tagagcaaaa
     2341 tgataaaaag acgttattag ctttctttga tttaggttca gcaaaaatga atttagagtt
     2401 agtagctgat ttttcagaaa aaaatattat cattaactct gtcccagtag tggagggagc
     2461 ttacaccgct gctgctcttc tacaggcagg tgctgacctg gatagtattc aatcacaatt
     2521 agctgaatta actatcaata aataaggaga tattatgact caatttcttg gggaattttt
     2581 aggaacattt attttggttc ttcttgggga tggcgtagtt gcaggaaatg tgctcagtaa
     2641 aaccaaagaa gaaggaactg gatggactgc tattgttttt ggctggggaa ttgcttgtac
     2701 cgttgctgtt tatgtttctg gattattcag tcccgctcat ctaaatccag cagtcacatt
     2761 agcaatggct tctattggag caatttcttg gggacaagtt atccccttta tcattgcaca
     2821 gatgttagga gcaatggttg cggcaactat tttatggtta cactattatc ctcattggaa
     2881 agaaacaaaa gatagtggac ttatccttgc cagcttctca accggacctg ctatccgcca
     2941 tacaccatcg aatctccttg gtgaaattat cggtactgct attcttgtga ttacaattat
     3001 ggcaattggt cctagtaaag ttgcagcagg tcttgatcca attatcgttg gtatagttat
     3061 tttcgcagta ggattctctc ttggtccaac aactggttat gctattaacc ctgcacgtga
     3121 cctagggcct cgtcttatgc atgctatctt acctattgaa aataagggta actcagattg
     3181 gtcatacgct tggattccag ttgtaggtcc aattatcggc ggtgtccttg gggctatctt
     3241 atacaatatt gctttgactt tagtttaact aaaaaaccag aaagttaata ctttctggtt
     3301 tttcatagtt gcagtatgct ttcaaaatag aaaaaacacc aaaatactta gtgttctttc
     3361 tggagatttt atgaaaaaag tttaggatgt ttatagtata accgccctta cttaacgttt
     3421 ccttaaaagc tcggttatta acattacttc ctaaattact atttatattc tccaagaaat
     3481 gactactatt aagctaaaaa gcgctattga catagcgctt tttagtctag tattatttag
     3541 tttcagtagt aactccatca aaatcttctt cattttcttc agcttcttct cttgtggcac
     3601 ggataatacc atctttgtga tgagctggac tgtaaattgt atagagtttt aacatctcag
     3661 tttcagaagt gttaattacg ttatgttcag cacctgccgg aactattata gcgtctccat
     3721 ctgcaacttc atattcatta ccatcaatga caaccttacc atgaccagct tcaaaacgga
     3781 aaaactgatc attttctgca tgaatctcgc taccaatttc tccaccaaca ggcagtgtca
     3841 ttaaaacgag ttgacaatgt tctgcggtat agaggacttg gcggaaattt gtatttccta
     3901 gtgtcaattt ttcgatattc ccatgaaatc ctttcataac gatacctacc tttctatgcc
     3961 tacagtctac tctttttgct ccttactgtc aaaaatttag ataagagtct gacattttta
     4021 ataatatcta ctgttccttg attcctatca tttcattgca aacctaaaat atagcctaat
     4081 gacaatagct atctatagct ttagaaacaa ataatgctgt tccctggcct ccccaattat
     4141 caatagactt ttggaaacgc tcatcttaga tgtacattat ttcaagactt gctaataaat
     4201 cgttagtgca gttgtaaaac tgagctgtga tataatcttg taaaatctga acttgttttt
     4261 gaactttctc atctgtcgga gaaagttctt tcaactgtcc aaagtcttta aaaatctgac
     4321 tcatagcttg tgaaatcatt gaaaaatcat tcttactatg gctattttca aattctttgt
     4381 aactatctgt atccccccca gcgtgttcta gcttcctctt gaaatacttc taattcttct
     4441 tgattataag ctgtaaaatc tgacatattt ttacccctct ggatagactt tgcatgttca
     4501 attacttctt ctagtcgctg tttctttagt tccagccacc taatttgatc aagaagtgct
     4561 tgattacgat cataatttgg actttccatt atctctttta tttttttaag aggaaattcc
     4621 agttccttaa ataacaaaat ttcttgtaac ttaatcagtg attcatcatc atagtaacga
     4681 taaccattct ctcctacaaa tgaaggagat aacaaatcta tttcatcata ataatgaagt
     4741 gtgcgcacac taataccaga aatgtgactt atttctttaa ctgttttcat aataacctct
     4801 ctgtaaagag tataatgtat aacgttaagt aagggtcaag ggaaaaatat tattttgtga
     4861 tgattaattt ataaagctta aatcaaaggt cactttgact aacaggttga taaaaaggga
     4921 tacgtcccca tgtttgctct gctatattag catttcccat ctgataaaca gaatctgctc
     4981 gttgcgtcaa actatcccac gtttgtgaac caatcctcgt cacgatatcc acagacttct
     5041 tcacagactt taaatgctgt tgaccttttt gtgtaaaacc taagacatga atagcatttg
     5101 gtataggact ttccacagca tttatcaaga tataggttaa taaacgtcga attcttgctt
     5161 tggtataccg tttagttgcg actttatcaa ctaactcatc cacagtctcc acataacgaa
     5221 tttgactttt tatacgattg gctatctctt cattcacttg ataaatttgt gtcaaatcag
     5281 aatgcgtcat tatctgatat ttgagtaaac taaaataatc ttgccaaact acttgtggac
     5341 tattcaaaaa caagtcactg ttaggcatac ctttttctac aaagaaacga tcagattgat
     5401 gtttacgaag acttgttgca gaagcaaata tctctgtttt ttctgatgaa tgataaccag
     5461 ctccttgacg ttgtatcgga tttaatcgaa taccattttg gctagctgct ttggtgtagg
     5521 ctaagcctaa aatgtgattt ggggtttgtc ccgaaaatgt aatccctgca aattcttgcc
     5581 acatagcttg cattttttgt ggataggaaa gagaatccga ttgttttttc aaaaaagctt
     5641 ccatctcctc ttttttattc acataaatat ccccaatacg ggcataatca agcatttctt
     5701 cggtaccgaa gcataagtta tccacaccta atcgtgccag gatagacaca gctccactag
     5761 cgaaataatc cgctgactgt accgaaacca gaaaagggag ttcaatcact aaatcagcac
     5821 cattttctag tgccatctgg gcacgggtcc atttatctac aatcgccggc tcaccacgtt
     5881 gcataaaatt acccgacatg gctattactt ttatgccttg agcttgttct aaaaggtact
     5941 tatgcccatt atgaaaggga ttaaattcgg caacaattcc tgtaactgtc atacttcttt
     6001 ctctttcaat tgattattta aagtaacagc atcaataata tctaggtgat aagttttacg
     6061 aaggtatatg agacgatctg tttctttttc aaatggtttt aatgccgata ataaatccaa
     6121 atgaagttta gggttcatca ataattccaa aataacttta gactttttct gacttacccg
     6181 acgttcaata atataaagta tcccaaatat catcagacaa gcgtataaga atattatcat
     6241 ctttttacct ccaaattatt acttatttat gagctacaaa aaaccagcga gcagaagttg
     6301 cagttggttt tttatcttca aaatctgcat acaccttaac atctttaaaa cctgcctgtt
     6361 caagtaaaat atcataagtc aaaatatcat aagtacgctc ttcatgaact tcatcatgac
     6421 gtgtaaatcg accatcttct tcttgaacaa aaaatgttaa ttcatggaca atagaatgag
     6481 gtgcatcatc ttcataagta tcccacacca tagcaaagtc atctgcattt tcatgataag
     6541 aataaccagg aaatacttta tctgtttgat aagtcgaatg aacatcaaaa ataaagacgc
     6601 cattttcttc taaggcctta tagacttcga taaaaacatc accaacctcg acttcatctt
     6661 gcatataaca aatagagtct gaatagcacg tgatgagatc atatttacca acattagata
     6721 aatctaacat atttccttca ataaactgga tactttggtg agcagaagtt gcacgttttt
     6781 tggcaagttt cagcatatcg ccactaagat caagtcctgt aacagcataa ccagcttgtg
     6841 caaatcgaac agactgaatt cccgtaccac atgctaattc caaaagtttt ttcttacctt
     6901 ttggaaaatg acgaagtgaa aaatcagtcc atttatcata taaagtatca tccatgacag
     6961 catcataaac agctgcaaaa gtttcatacg ttgccatata atagttctca aatctttctt
     7021 gatattacat ctctcaaaaa gcagtcccaa gactgcttag ctttattctg tcataaagac
     7081 ttcagcatca agcaatggtg cttcatgcca aagtttttca aggttatagt gctgtctctc
     7141 atcttcagag aagatatgaa ccacgacgct attgaggtca agaagtaccc aaccagcttt
     7201 actatcgcct tcaaggtggc tagcatcccc accatttcct tttacttgtt cacggatatt
     7261 gtctgcgatt gcttctaact gcctactatt acttgcactc ataatcacaa agtaatctgc
     7321 aacactcgtt acaggctgta aatctaaaat tacaatatct tctgctcgtt tttcatccgc
     7381 agccttaacg actagctgta gtaagtcttt ttctgtcatt ttattcctcg tttattaatt
     7441 tcaataagct atcttttgct accattgtcg caaaacgatg atgccaaata ttctcgtaat
     7501 gttttttaat agaatcccca cttaaagttg gtgtatcaaa tgttaggtgt ccaccttgtg
     7561 gaataaagat atcataacca tattcaaaag caacttttac tgatgtatca aaacagtatt
     7621 ctgtttgaag tcccatcatg attaattgac taatatgctt actttgcaag taatcatgta
     7681 aatgagtatc tttaaaacaa ctattgtaag ttttatcaat tattttgtga tgcgtcacaa
     7741 gatgtcgctg ttcccaaatt tcccactgtg gagaaccttg aggcaattcc ttatcatgat
     7801 gccttatcca tatcacttca caattagaac ctttggcact taaaagtaaa tcatctaaaa
     7861 gctctgcaaa atttgttaaa tgctttggtt tcttatccat aataccttgt tggatatcaa
     7921 ttagtaaaag tgcacaagtc atactttcct ccaaaatgac cactgcatcg ccaaacctat
     7981 catgaccatt attcaataaa agaattagtc gagataaggt atataagcat tataggtttc
     8041 aatcgtttta ggatagatag gctgagcctt acttgctaaa aacgcaactg ttctagcagt
     8101 ctcataagct actgctttat taaggtcaac tttcgctaac tcacgagctt cttcaactcc
     8161 tggaaaatct cgattgtgtt caatgtaatc tgctacataa acgattttat ccaatgtgga
     8221 catttgggct gaaccaacag tatgttttgc aatagcagct aaaatatctt gatctttgat
     8281 agctagatcc tcttgtattt tataaatgcc aacaagacca tgccaaatat tattacccca
     8341 tttttttagg tcgggatcta gttggtattt atcaattaat ctaagaaatt catcatcaga
     8401 tagttcctta gcataatcat gtaataaagc agctagaccc gctttttcct tgtcataacc
     8461 ataccgttct gctaactcta ttgcagcacg ctctactcct aacacatgat taaaacgttt
     8521 gtcggacatc atatgacgca ctttactcaa aagttcagtc cgatctaaac ctgtgtaatc
     8581 tttgtaggtc atttgtaaag accttctttc ctgatataat ctaatacctc tctaggcagt
     8641 aaatagttgg gctgacgatt acttttaata aattgacgaa tcatacttga tgagatatcc
     8701 atcaatggca gatccaccca aataacggga tatgatgtac cagctttata ttttggacgt
     8761 tgaacaccaa caaactgcac cattttaaca agttcatcaa tacgatgcca cttaggtaga
     8821 tattccacca tatctgcccc aataatgaaa taataatcaa catctggatt tttttcaatt
     8881 agaagcttca tcgtatcgta tgtatagctg atacatttac gttcaatttc aattggttca
     8941 atagataaac catcaatgcc ttctattgct aattcaagca tcttgagacg atgctgttca
     9001 tcaattgtct cttttttatc aatatgtggt ggctgaaatt caggcatcaa cagaacttgg
     9061 tctaaacaca attgctgacg tacttgatct gcaacaacaa ggtgggcgtt atgaacaggg
     9121 ttaaaatttc ctcccataat tccaatttgt tttcggttag tatctcgctt tttctcttct
     9181 aattcaactt ttgtaaatgg tgttagcaat tcgagtgcca taattcctcc atgaacaagt
     9241 agtatctcta aaaaaacaaa cactgattat catgatcagc tatcatacgg aagtgtaact
     9301 taggtaaatc gttttttagt tctatactct atttcttaat ctattgtagt ttagtttgat
     9361 tttactgctt ttactttaac tgaaatttta cgattttctt tgcgcgctga ctctttataa
     9421 agaatcaaga tacgtcctat tttaaggaca gtatcgcagc caatttcatc ttccagaact
     9481 tcagcgacat cgtggatatc ttcatcggta ttttgtaaaa gggttactct gattagctca
     9541 cgtgcatcga gtgcattacg tacgcttgtt ttgatttgat catttaaacc atttttccca
     9601 atttgaataa tgggtttcat agaatgtgct tctgatttta agaaagcacg ttgtttagat
     9661 gttaacatat tagtttcctt tttatctatt ataggattat gactgtatca tcagctaaat
     9721 tagatgaaaa accgtcatat gttgctggtt aatttagtta ttaaattaaa gctttgcgta
     9781 aaacaactgc cacgccttct ggagcccaag cagctactac tactggattt tctgcttctg
     9841 atttcacacg tatccaacca agacccgaat aaacaatatc cattttatct ttgattgtaa
     9901 attcatgacg aactaattta ggaaaatctg aaacttcctt acctgttggt ggtgtcaata
     9961 attttccaac atgtttagtg taaaattcat ctgctccaac tagtttagtg cggtgtaaat
    10021 tcaggttatt atcaaaatat gccgtaaaac cttgtttttg accagaaata aaatcaaaac
    10081 gtgctaggcc agccaagaat aatgtttgtt ctgaattaag ctgataagtt ttgggtttta
    10141 tttctttttt aggactaaca tatttaagat tttttgctgt caaataatga gccatttgat
    10201 gacgatgaat aatacccggg gtatcaaaaa tatagctacc atcatcaaga ggtatttcta
    10261 ttttatctaa cgttgtacct gggaaacgtg aggtcgtaat aacatcacga ctaccagtaa
    10321 tttcacgaat aatagcgtta ataagagttg actttccaac attagtaacc ccaacaacat
    10381 aaacatctcg accatgacga tacttttcaa tcgtatcaat caaatcctta atagcatgat
    10441 gattttgggc acttgtcaag atgacatcaa caggacgaag cccctcttca tgagctctct
    10501 cagttaacca ttgtgttact tttccatctt taaccgattt gggaagaata tcttttttat
    10561 ttcctactaa aagaacatcg tttcctgcaa cgaaacgact aagaccagga ataattgaac
    10621 cattgaaatc aaaaatatca atgacattca ccacaagggc atcactatct ccaacctcat
    10681 gtagtaattt caaaaattca tcatcagtga tatgaacgtc agtaatctca ttatagtggc
    10741 gcaatctgaa acaacgctga cagtataatt ctcctgtctc taaacctttt tcaagtgctg
    10801 cacgaggtgt atagccagca gcatctttat tttcagtttg aatcctggct ccgcagccaa
    10861 tacaaaataa ctcttccatt aaattccttt ttgatatacg attttaccgt aattctcttc
    10921 tatttttttc caaacgcgac gttcacgtaa gcgattgaac ttggtattcc aagcatcaga
    10981 tttaacaatt ggttttacca atacggactt tattcctgcg cgataactag cacgaatatc
    11041 tgtcatcaat tgatctccaa ccataatcac ctcgtcacga tcaaagccat aacgctcaat
    11101 tgccatgttt ataccacgag taaatggttt catcgcacga cttacaaaat ccacaccaaa
    11161 acgtgatact gcacgttcta cgcgagcatg attgttatta gacactacta caactgaaat
    11221 atccgctgtt gtcatctcat ctaaccatgc acgcacctca gcagtaccat ctggattatt
    11281 ccaagcaatg agtgtattat ctaaatcaac taaaaccgca cgaatgccat gacgtaataa
    11341 atcttctgct cttaaatcat aaactgcctc aaccacgaag gtcggcctat aatcatctat
    11401 actcaatttc atctccaaaa aatatatttt actattcatt ctaccataat tcttaagaga
    11461 tagcgagtaa agaaaaaagg tctttaaaga cctttaaatt agggtttcaa ccccaaaaga
    11521 tactatctta ctcgtttcat tttcttcaat gcaattaaat ctttgtaaga gattagagtt
    11581 acagcaagaa aaataaacag catcccaagt aaatctattg gataaaaaat ttctcctaaa
    11641 acaagtactg tcaaaaatac cgaagaaact ggctctaccg atgctaataa gctacccttg
    11701 acagcaccta caatactaac ccctttaagg aaaaaagtgt aagcaaaaat agtcccaata
    11761 ccaataatgc caatataagc aagtatactc atcacattaa tttgtaaggg gtattgccac
    11821 gcttttgtca ccaaactaaa taaaatccca cctataaaca ttcctgaacc aataacaatc
    11881 gtacttcccc attcatgaat aagtttcaca ggtaataaaa tataaataga ataagtaatg
    11941 gcggaaccaa gcccccacat taatcctttc ggtgtgatag ctaaacttcc taacttccca
    12001 tgcgttgcaa tcatataggt tcctaaaatt gccatagcaa ttgctattat ttcaaaagat
    12061 gttggtcttt gtctgtttag gatacaaaca atactcaaaa taagtattgg tgccatatat
    12121 tgcaaaaccg ttgcagttcc agcatttgtt aaatgaatcg ctctgagaaa agcatattga
    12181 tttaaaacta aaccaaatat agaaaaaagt aaaacctgct ttaaaaattt tggctgtttc
    12241 cacgctgcta ctaaatgctc cttttgtttt gatctagcta aagacaagag aaagatacct
    12301 gttatcaaca atcgtaaaga agtcaaaagg ttaacgtgta ctccgtgcga catcaagtat
    12361 tgaccactaa ttccagaaat gccccatgct agtccggcag ctaaggtcat cattgttcct
    12421 ttttcttttt tagtcatttt tcccctcatt tttatttctt aaacactgta gttaaagtca
    12481 tctgataaat atcttagctt cttaattatt tatccctagg tgttcgtgct aaattagcga
    12541 ataaaaacca gccaaaaggc tgatttctat tcttctttta attttgctaa ttcttgcgct
    12601 aacagcttca gagcaacttg tggattagct tgtccttttg ttgctttcat tagataacct
    12661 gtgaaggcct tatcagcatt gcgttttcct gacttaaagt caatcacagc agcttcatta
    12721 tctgcaaaga cttgatgaat aattgggatt aatacttcag gatctgaaat ttgaactaaa
    12781 ccagcctttt taacgaattc ttcagctgaa ccaccatttt tagctaaatg cacaaaaact
    12841 tttttagcaa ttttggatga aattgtaccg tcagcaatca agccaatcat ctcaaccaaa
    12901 ttctcaggag tcagaccaat ttcttcgatt gattttgatt cactgttaag aaattgcgcc
    12961 acttctcctt gtaaccaatt ggaaacttgt tttgcatcac ccccaatagc aacagctttt
    13021 tcaaagaaat cagaagttgc ttttgtagct gttaattggc tagcatcata agatgataga
    13081 ccaaaagacg atacatattt cgcacgacgt tcctgtggaa attctggtaa ttctaaacga
    13141 acttgatcaa tccattcatc cgagatatca aatagtggta gatctggttc agggaaatag
    13201 cgataatctg atgagccttc cttgacacgc atcaaaatag tctcacctgt tgtttcatca
    13261 aaacgacgag tctcttgctg aatttgacca ccagatcgta aaacttgtgc ttgacgtttt
    13321 tcttcatgta tcaaaccttt acgtacatta ttgaatgagt taaggttctt aagttctgct
    13381 ttagtaccaa actcttcttg accataagga cgaagtgaaa tattggcatc tacacgcata
    13441 gaaccttctt ccattttaac gtcagaaatt ccagtatatt ggataatctc tttgagagct
    13501 gtcaaataag cataagcttc ctcaggagaa cgcatatcag cttctgatac aatttcaatc
    13561 agtggaacac cttgtctatt aaggtcaaca taggaataac catctgtccc atgtgtattt
    13621 tttcctgcat cttcttcgag gtgggcacgc tcaatacgaa ttttttttcg tgtaccatct
    13681 tctaattcga tttcaatcca accattgtaa ccaataggct catcaaactg tgaaatttga
    13741 taagctttag gattatctgg atagaaataa tttttacgat caaaatgcat gttttgatgg
    13801 atatccatat tcaaggcaag tgccgccttg attcctgcat caataactcc tttattcatg
    13861 actggaagga cacctgggaa agaccagtca attacatttg tattagcatt ttgttcctga
    13921 ccaaaatgag cggaagaagg tgagaaaatt ttagagttcg tatttaattc aacatggact
    13981 tcaagtccaa tgactgtttc aaaattcatt agttctcacc tccaaaaatt ttaggttgtt
    14041 gtttatgata atctgtcgtt gcttcgaagg cagcagcaac ctgatatatt gtttcttcac
    14101 tgaatttcgg accaatcaac tgcataccaa ccggtagacc ttgatcaaaa ccagcaggga
    14161 ttgaaatacc tggtaaacca gccaaattaa ctggtattgt tagaatatca gctaggtaca
    14221 ttgcaactgg atcgtgattt aaagaatcta agtcaaaggc tgttgttgga gctgttggac
    14281 ctaaaatcaa gtcataatct gcgaaaactt tttcaaaatc ttgaataatc aaactacgaa
    14341 cttgacctgc ttttttataa taagcatcgt aatacccaga agataaactg aacgttccca
    14401 gcataatgcg acgcttaact tcgtctccaa aaccttcaga tcgtgtattg acatagatat
    14461 catccaagtt tttataattt tctgtacggt atccataacg aataccatca aaacgttgta
    14521 agtttgaaga agcttccgat gaagcaacaa tataataaac agcaacaccg tatttagaat
    14581 gcggtaaact aacttcctca ataactgcac ctagtttttc cagatgtttt gcggccttaa
    14641 taatcgtttc tttaactcct tgcgctattc cttctcccaa atattctttt ggtaaagcaa
    14701 ttttcattcc ttgaatatct tgaccaattt tagcagtaaa atcgcctaca gtacgctcag
    14761 aagaagttga atctcttaca tcatgcccag aaataactgt caaaagctgt gcattctctt
    14821 ttactgtttg agacatcggt ccaatctggt caagcgaact accgaaagca aacaaaccaa
    14881 agcgtgatac acgtccgtaa gttggcttca taccaacgat tccattaaat gaagccggtt
    14941 ggcgaattga accacctgta tcagagccca atgataaacg tacttgtcct gaagctactg
    15001 ctgctgcaga accacctgat gatcctcccg gaactttact atggtcccaa gcgttatttg
    15061 tctttttaaa ataagatgtt tctgtagagc ctcccatggc aaattcatcc atgtttgctt
    15121 taccaatcac aatcatatct ttagcatata acttttcaac agccgtagca tcaaagattg
    15181 gctcatagtt atagagcatt ttagaagcag ctgttgtaag aatccccttt gttgaaatat
    15241 tatctttaac tgctaaggga atacctgaca taacattatc agcatcaatt cctttatcat
    15301 caatttcttt ggcttgagct attgccatct catcagaaat agtaataaaa gatccaaccg
    15361 cttgttcacg cgcatgaata tcttccaatg tagcttttgt taattcagtt gctgaaattt
    15421 cttttttaac gagaaaatca tgcaactgat caattgattg attattaaac gacattaggc
    15481 atctcctcca tcctcaagaa ttgcaggtac tttaatataa taatcttgat gctgtggtac
    15541 atttttaaat aagtcatcac gattatggcc tggttgagca atatcttcac gcatcacagt
    15601 tttgcgatct gccatggtcg ttgttactgg tacgccttct gtgtcaactt catttaagag
    15661 ttcaatcata tcaacaatct ttgacaaact tgacgcaaat tctttagtct cttgatcaga
    15721 aaagcgcaat tttgacagat gggcaacatg acgcacttct tcttcagaaa ttttcatgag
    15781 ttctccttat ctctgtatca ttttcgtttt aagatagtct ctactattat agcatattaa
    15841 actctcaatg ttatcacatt atcccagctt ttcataataa acgcttctta cctatgataa
    15901 gaagcgttta ctgtttattt atctattgtt atatcatttc tgctttgttt gattttagcc
    15961 tgtgcagccg ctaatttagc aataggaatc ctaaacggcg aacacgatac ataatgtaac
    16021 ccctgttggt aacagaactc aatagatgat ggttctccac catgctctcc acagatacct
    16081 attttaaggt ttggcttaac ttcttttcct aacctcactg cctgctcaat aaaccttcct
    16141 attccttttt gatctaaaac ttggaaaggg tcttttttta gaagtccttt atcaacgtat
    16201 tctcctaaga attttccagc atcatctcgt gaaaatccaa agctcatttg tgttaaatca
    16261 ttggtcccaa aactaaagaa atccgcaaac tttgcaatat catccgctat catacaagct
    16321 cttggtgttt caatcattgt cccaataggg aatgataagt taattttttt agatttactc
    16381 tcctcaacaa ttgtctcttc aatcagatca cgtattatag agatttcttc atgcgctgtc
    16441 actaaaggaa tcataatttc tggctttgcc tcatatccct ctttcattgc aagtatagcg
    16501 ccttgtacaa gagctctcac ctgcatttgg taaatttccg gataggtaat cgcaagtcta
    16561 caaccacgat gacctaacat agggttgaat tcttctaatg tttgaatccg tttttcaata
    16621 gctaaagtag atatccccat ttgtcttgcc atagattcta tagactcttt atcatggggt
    16681 aagaattcat gtaaaggagg gtcaagcaaa cgaatcgtac aagctttacc tttcaatact
    16741 ttaaaaattt gataaaaatc gtctctttgg aaagataaaa gcctttctag agccttcctt
    16801 cggctcaata tttcatccgc taaaatcatt tctctaacga ctggaatacg ttcatcgtca
    16861 aaaaacatat gttccgtgcg acaaagacct attccttctg ctccaaagtc caaggccttt
    16921 tgagcatctc tcggattatc tgcatttgaa cacaccatca tatctcgatt ttcatctacc
    16981 catttcataa atatctcaaa atcacgatct acgctagtct ctgccatcgg aacttgtcct
    17041 atataaacat ttcctgtagc accatcgatt gataacatct caccttcttt tatcgtttga
    17101 tgcccaatag aaatttctcg tcttacttca tcaaccaata attgactaca gcctgcaacg
    17161 catggtttcc ccattcctct ggctactact gccgcatggg aagtcattcc gcctcttgct
    17221 gttataatcc cgtttgcact aaccatcccc tcgatatctt caggtgatgt ctcttgtctg
    17281 accaatagca ctggatttcc ctttttcatc tctttaacaa catcttctgc atggaaataa
    17341 actttcccat aagcagctcc aggagaagct ggaagtcctg tcgtcaaaat aatagctttc
    17401 tttaaacttt tcaagtcaaa agaagggtgt aataactgat ctaattgttt gggctcgata
    17461 cgtagtatag cttcttcttt actaattagg ccttcattta cttgatctac tgctattttg
    17521 atagctgcct tcgctgtacg cttaccgctt ctcgtctgaa gcatataaag attaccattt
    17581 tcaattgtaa actccatatc ttgcatatcg cgataatgag cttctaactt ttgggtaacg
    17641 cttacaaact cttggtaaat aataggcatt tgttctgcca attttgatat ggattgtggt
    17701 gttctgatac ctgcaacaac atcttctcct tgtgcattaa tcaaatactc accaaataaa
    17761 tttgctgctc cagttgatgg gtttcgtgta aaagcaactc ccgttccgct gttatttccc
    17821 atattcccaa aaaccatagc ttggatatta acggctgtcc caagcgtctc cggaatatca
    17881 tttaatttac gataaatctt agctcttgga ttattccatg atttaaaaac tgcctcaatt
    17941 gctaacaaaa gctgcctttt aggatcttgc ggaaattttt ctcctgcttc tttttgatat
    18001 agttccttgt aaaattcaac aagtctcttt aaatctgaac cagttagttc tgtgtcatct
    18061 tgataacatt tatctgtctt aagtcgatct aaaatggtat caaatttata ttttggaatg
    18121 cctgtaacca catctgcaaa catttggata aaacgacgat aactgtcata agcaaaacgc
    18181 tcgtcttctg tctttttcac taaaccttgg acactacgat catttaaccc taaatttaaa
    18241 actgtgtcca tcataccagg cattgaaaaa actgatcctg accgaactga caccaacaaa
    18301 ggatcatcaa cacttcctaa ttgcttgttc tgttcaacct ccaattgtgc cattgcttga
    18361 tcaatttgac taagaatact ctctcggata tggcaagcgt tatcataata atcattacaa
    18421 gcctgtgttg taatagtaaa accttgtggt accggtaacc caatactcgt catttcagct
    18481 agattagccc ccttaccacc tagcagttcc ttcatttcct tacaaccttc atcaaaatga
    18541 tacacaaact tagtttccat cagcgtctcc ttttacagat ttttagctac ttgtccattt
    18601 gtttttaaga tttctaaaat aatcgttgct gtttcttcga ttgctttatc agatacatta
    18661 atgattgggc aacctaaatt tttcataact tcttcagcat acctcgtctc ctccaaaatc
    18721 cgttctaaac tggcataatt tgccgtacta gaaagaccta aggactttaa tcgaactttt
    18781 cggacttgat taagatgatc aaccgaatta gttagtccga taatccttct agaatcaatc
    18841 atacgtaatt ctttaggaac aggaacctct ggaaccaaag gaatattaat aactttaata
    18901 tttttatctg ctaaaaacat acttaatggt gttttagaag ttcttgaaat tcctataata
    18961 actaaatctg cttgtaaaat acctcgcgga tctctcccat catcatattt tactgcaaat
    19021 tctattgatt ctactctttt gaaataatca ttgtctaagc gtcgtaaaat ccctggttct
    19081 cccaaaggat ctatacccga tatccttgat attccttgaa taacatttgt taataagtca
    19141 acataagcat aatgttcact ttcacatcgt ttctgtgcat aggaagccaa agcaacatca
    19201 actaaactaa acatcataaa tactgtcttc tggctagcct cttcaaaaac ttgttctaag
    19261 cgttcctgac tgttaatata ggaaaaacgt tgaaaatgcc aatcatcatg tcctggaaat
    19321 tgtgacaaac atgctttcgc aatagctttg gctgtttccc ctaaagaatc tgaaataata
    19381 aatatagtga gttgatcttc catcagtttc ctcctaataa tctcttattt ctaaaaacaa
    19441 actagcgagg attgttttag ataatttccc gatcacttta aatttttcgg gatattgctt
    19501 atcatgacga acgacaggga gactatccac ttttctgctc actaatttat cagctgccgc
    19561 aaaaagactt tcattttcta aaacagttgt cacgtgtggc atacgtgtca tcaccattcc
    19621 tattggcatt ttagaaagat ctcctccgcc aatactggtt tttagtaaat ctttacgtga
    19681 cacgactcca cagagaaaat catcatcatc caagataaaa gcacaaccag catcttccat
    19741 aaaaatatgt acaataacat cataaactga atctttttga tgaactgtca gagggatccc
    19801 cataatttct gaaactgtca tcttttcaaa atgacttgtc cctattgaag catgatactg
    19861 tcctaaataa aaataaccaa ccttaggttt tgcatctatg atacctagca tacttaaaac
    19921 cgccaaatca gaccttagtg ctgctcttgt tactcctaat ttctcagcta ttagctcacc
    19981 agtaatgggc tgaaactgtt tgataatatc aataattctt tcttgccttt gatttaattg
    20041 aataagatca ccttctttat atattgtata ctatttattt tatatgttat attatatatc
    20101 tttttttact gaaatcaagt aaaaaaagat actattttaa aaataatcat actatatatt
    20161 tgcttttaga catatctttt gttaaacttt cttcgaaaac gttttcgaat ataagtatcg
    20221 gaggttgtaa tatgacaatt aaaaatctca ctgttgcagg aagtggcgtt ctaggtagtc
    20281 aaatcgcctt tcaagcagct tacaaaggaa tgtctgtcac catatatgac attaacgatg
    20341 aagctttaaa taagggaaaa gaacgcatca aaaaattagc taaagtctat caatcagaaa
    20401 ttgaaacagc aaaagaagca tatagtgata aagctaaaag tatcaagtat aataaaaact
    20461 tattgcctag cttagaccat atttttcttt caaaagttgc cgattcatta gacttgattg
    20521 ctgatttacc aaaccaaatt actttttcaa aaaacttaga ccaagctgta agtgacgccg
    20581 atttagtaat cgaagctgtc ccagaaacag ttagtataaa agaagatttt tacaaacaat
    20641 tagctaaagt ggcaccttca aagacaatct ttgcaacaaa ttcttcaaca cttgttccaa
    20701 gtcagtttgc tgatataaca ggacggcctg acaaattttt agcaatgcat ttcgctaaca
    20761 atatttggca aaataatatt gttgaaatta tgggacataa gggaactgac gacgaggttg
    20821 tcaaagaagc tttggcattc tcaaaagata ttggtatggt acctttatat attcataaag
    20881 aacaacctgg ctatattctt aattcaatac tagttccatt cttagagagt gcattagcat
    20941 tgtactataa taaggtttct gattctgaaa ctattgataa aacctggaag ttaggaacag
    21001 gagcaccgat gggaccttta gaaattttag atattattgg catcgatacc gcttataata
    21061 tcatgaagaa ttattcagat accaccagcg atcctaatag tttacatgct caccttgcaa
    21121 aaatgcttaa agaagaattc attgataaag gacgtaccgg taaagctgct ggacacggct
    21181 tttatgatta cgactaaact ataaaagagg ttagataaaa atctaacctc ttttataatt
    21241 caactaaaga atagttgcac caagaacagt tttaaaatga tttaaagccc attgatgatt
    21301 actatcattt aagctggcta cagcagctgc tgggacctca attttataac ctaagttata
    21361 agcatcaatt gctgtgtgta atacacaaat atctgtcagt actcctgtca atatcaaagt
    21421 atctactcgt ctttcacgta ggcgaatatc taaatcagta ccagaaaagg cagaataatg
    21481 gcgcttatct atccaaaaaa cacgactatc ttccttaata gtttcatata gagttcccaa
    21541 aggaccatat aggtttctac cagaagtccc tttaatatta tgctcaggaa aaagattggt
    21601 ctctggatga aaaacatctc caatatcatg attatcaatc gcaaaaaaaa tataatcccc
    21661 tgaccggtat gctttctctg taacgtcagc tatagctgaa gcgatagatt gcgctggttt
    21721 acctgccgtt aatttcccat catctgccac aaaatcatat gtgtagtcta ttgagatgag
    21781 tgcttttgtc atttttcttc ttatctcctc ttgatttcaa aaaaggaact gaaagctact
    21841 cccagtccca tgctatgatt aattatattc ttttaatttg tcaaaaatac cttcgttaat
    21901 aactttgagg tatgttccct tcataccgag tgagcggctc tcaatgattc ccgcagactc
    21961 caatttacga agagcattta caataacgga acgtgtaatt ccaatacggt ctgcaataac
    22021 agatgccgtt aggcgtcctt caagtccatc taattctcct aagatagcag ctacagcttt
    22081 catttctgaa taagataaag tgttgatagc cattgtcacc gctgtttgtt tacgaatatt
    22141 ttcttctaga ttttctgttt gaaggttaag taattgaata ccaacaacgg tgcttgcaat
    22201 ttcaactaaa atcaaatcat cgtcgctaaa ttctttatca ttacgccaga taataaatgt
    22261 tcccaagcgc attccaccac cataaattgg agcgattgtt gtaataccat cttggaagtt
    22321 ttcctttgtc tcaactggga aaattgacaa atcattatct actgataagt tagcttctgt
    22381 atcataaaca cgactagcag atttaacata gtaatctggg aattgttttg tttcaaaaaa
    22441 ttcttccaca cggtcagtgt ttgttttata tttcatggca tagccaagta agttaccacc
    22501 accattaata atacatgcgt tacagtcaat aatatcagct aattgagccg ccattgtatt
    22561 atatggtaat tcggcatcta agctatctac ggaacgctgt aagatggatg taatttttct
    22621 agttttttct aataaattcg gcatttcctt catcctttgt atgttttgcg ttacaattat
    22681 atcaaagcat tttagctttt gcaaatcgat atcagtacat tttaagaact tctattgtta
    22741 atttaatgtt tatctaataa aaattcattc tagcgcttgt attggcttaa aaagcgagcc
    22801 attttttctt gtagttcagt caattcatca acacgtggta agtaaacaat tctaaaatgg
    22861 tcagcggtat tcatattaaa tcctctaccg tgtgttagta atactttttc ttgttttaag
    22921 aaattaagca caaactcttc atcattatca atacggtaca tatctgtatc aattttaggg
    22981 aaaagataca gtccagcatt tggttttaca gcagataaac caggtatttc attaattgct
    23041 ttatggatat aattacgttg ctcgtagata cgacctcctg gcaacaacat actatcaata
    23101 gattgctgtc cgcctaacga tgtttgaata acctgttgag caagaacatt tgaacacagc
    23161 ctcatgttag ctaacatatt caaaccttca atataacctt taacatgttg tcgcggacca
    23221 gacaatacca tccaaccaac gcggaaacca cagatgcgat gtgatttaga taagccagat
    23281 aaagtaactg taaaaatatc ctctgcaata gaagcaattg ggatatgctc cattccatcc
    23341 ataactaaac ggtcataaac ctcgtctgaa aaaataatca agtcattttg tctagcgata
    23401 tccacaatct cttgcagaat ttcacgcgga tagactgctc cagttggatt atttggatta
    23461 atgaggacga ttgcttttgt tttggatgtg atttttgatt tgatatcatc aatatcagga
    23521 taccaattag cttcctcatc acaaatataa tgcacagcat ttcctccagc aaggctaaca
    23581 caagctgtcc ataacgggta atctggcatt ggaactagaa cctcgtcatc attatcaagc
    23641 aaagcttgca ttgacataga gattccctca ctaacaccat taacaatgta tatatcatcc
    23701 atgtcaacat ggatgttttg taactggtaa tactgcataa cagccttacg agctgaaaaa
    23761 atacctttcg aatcagagta accttctgat tcacgcgcat tcgtaattaa atcacgaatg
    23821 acttcatctg gcgcttcgaa gccaaaggca gctggatttc cagtatttaa ccgcaatatc
    23881 ttttcaccat ttgccctcat gcggtccgcc tcttctaaaa cagggcctct aatatcataa
    23941 gccacatgtt ccagcttcat tgatttatca aatatcttca taaaaatacc tcgtatcttc
    24001 cattataaaa caaatattct caaaattgaa acatttactg cggtttatca agcgattaag
    24061 ttaatattaa gctatttcag aaaactaaca taaaacgctt tcatttttct agaaaaaacg
    24121 ttataatata gctataaaat gaataaccaa catatctaac ttactcatca ctcactcctt
    24181 ctgataagat ggctagttga gtgcaagagg ttatctcttt tggttatatt tagaggagga
    24241 caggcttatg tcacaaaaat atgaacgcat ccttgtcgct attgatggat caacagaatc
    24301 tgaacttgct tttgaaaagg cagttaacgt tgctcttcgc aatgattctg aattaatctt
    24361 gacacatgtt atagatacac gtgctctaca aagctttgcg acatttgata cttatatcta
    24421 tgaaaaatta gaaaaagaag caaaagatgt ccttgaggaa tatgaaaaac aagctcgtga
    24481 aaaaggagca gataaagtta gacaagtcat tgaatttggt aatcccaaaa cattacttgc
    24541 acatgatatt ccagaaaaag aaaaagtaga cttgattatg gttggtgcaa caggtcttaa
    24601 tacctttgaa cgtttcctta tcggatcttc ctcagaatac atccttcgtc acgctaaagt
    24661 agatttactc attgtacgtg accctaataa aacaatgtaa atttaatacg ctcactttct
    24721 aaaatatcct gagttatatt aactcaggat atttttattt tcgatttaag gatttctgaa
    24781 tttggctatc taattcacgt cgaattgatg gctcaggagc gaaatgttct tcccagtcgt
    24841 ctggttttaa aattttatga gtaacaggat caaaatgagc tttgccatct ggaaagattt
    24901 tacccatatt ggcctcatgt actgtatcaa aaagaggttt tgggtcgact cccataagaa
    24961 caaaggagcc ataagtcaga taaagtaaat ctgttaaggc atccacttct cccactaatg
    25021 gtgtttctgg atggtcttta gaaataacct tatctcttgc tttatcaata gctaagtgta
    25081 aattacggat agattggtca aatacttctt ggttaccttg actagcagca tacaagaact
    25141 caacgatttc ttctacttta aagtccgacc taaaaccagc ttccttactg tcatataagc
    25201 gtggcgtttc aatagtttca ccatccatta atagatggaa agattttact ttattgaaat
    25261 tctcgtcacg actactgaat gttttttcaa tctcaaattg gataagtcca taatgggcta
    25321 atgccttaga gataccatca tcattattgt cagcagttgt ataacgtgca ttttctttca
    25381 cttgctgact agcgttcccc atggcaacac caataccaac ttgagataac atctcaatat
    25441 cattatcaga atctccaaat gctatcactt ctgataaatc aaaaccatag cattgaccaa
    25501 cacgttcaat tcccttaact tttgactgcc cttcagaaat gacatccatt gagtaaggac
    25561 tgcttcttgt taatttaaca cgaggaaatt gtttttgaat tcgctctgat tcgccctctg
    25621 ttgcaactaa aacgacctga taaataggtt ctcgtaagat aaccatgagg ctatcaatat
    25681 tttgaggttt aatccgacga ataaaatgct taaagcttcg ttcaatcgct tttgcccatt
    25741 ttctcggaac aaggctagat acaatctgcc ccaaacggct agttcctaga ccaataatac
    25801 ctgaaccgag taatcctgag gctgttccta atgaaatttc tcttcttctt gctccagcat
    25861 aacgaatcag acgatagaca gttgtcttag ataattgatt ggtataaata atttcacttc
    25921 tactatagat atattgacca ttataggtta cagcaaaatc taaaccaagg ttttctaaaa
    25981 aaggttgtac aaaactagga cctcgccccg ttgctaaacc gactagtatt ccttggtctt
    26041 tcaaatttcg aattgcttta attgttgact tttgtacatt cttacgatca ttaagaagtg
    26101 taccatcaat atcaaaaaat actgccttaa ttgccactgg gtcaacatcc cctctttttt
    26161 atgataaaat aaattatatc acaacttgaa agaagtataa aatcaatgaa aaaaatcctt
    26221 gttctacaca caggtggaac gatttccatg aatgctaacg agaagggaca ggtcatgtcc
    26281 agcgccgata acccaatgaa atacgttgat ttatcattag atgaccttga tttgactgtt
    26341 gtagattttc taaatcttcc cagtcctcaa atcacccctc atcatatgct tgatatctat
    26401 cactatctga agcaacatgc cagtaatttt gatggagttg tcattacgca tgggactgac
    26461 actttagaag aaacagcata ttttttagac acaatgatac tccctaaaat cccaatcatc
    26521 attaccggtg ctatgagaag cacaaatgaa cttggtagcg atggcgttta taattaccta
    26581 tcagccctcc gtgtcgctaa tagtactaaa gctgccgaca agggggtgct agttgtcatg
    26641 aatgatgaga tacatgctgc taaatatgtc acaaaaacac atacaacaaa cgtttcgaca
    26701 ttccaaaccc ctacacatgg cccccttggg attattatga aacaagacct gcttttcttt
    26761 aaagcaactg aagagagagt tcgttttgat ttagataaaa ttacaggaac tgttcctatc
    26821 gtgaaagcat atgctggcat gggtgatagt ggtatcatta gctttttaaa ttctcaaaat
    26881 attagcggat tagttattga agcactcggt gcagggaata tgcccccaaa agctgcacaa
    26941 gaaatcgaag aattaattga gcaaggtgtc cctgttgttc tagtgtctcg ctgttttaat
    27001 ggtattgctg agccagttta cggatatgaa ggtggcggag caaaattaca agaatctggt
    27061 gtcatgtttg ttaaagaact aaacgctccc aaagccagac tcaaattact cattgcactc
    27121 aatgcaggtc tcacagggca aaatctaaaa gactatattg aaggttgata aaaaactcta
    27181 gaaatatttc tagagttttt tattaatttt cagtttgtaa tactctctca acactgctaa
    27241 taggcatttc ttgattcgtt attaattgaa atgcttctgc tccttgataa atcagcatcc
    27301 ctagaccatt ataagcatgt ttcactccat tttgtctaac aaatcgtaac aatgctgttt
    27361 ctttaggctt gtaaaccaag tcgacaacta ctaaattcgg tgttaaaatt tctggatcgt
    27421 taattaaact ataattatct aatggcctca ttcctaaact agtagcatca atataaaaat
    27481 gactagttct aatagcgtct ttaaatgctg tcttattttc tagataatca acgactacct
    27541 ttatttgaaa ctgtttttta attttatctg ataagtcaat gaccttatcg taatttgagc
    27601 tgttacgatt aaataatcta atttccgcaa ctccctccat agctgcttga actgcaactg
    27661 ctttacctga accaccaata ccagctattg taattatttt atttttagca ctgaaacctt
    27721 gagctgctaa agctttaaaa caaccaatgc catctgtcat atggcctact aaacgtccgg
    27781 ttccaccttg attaacgata gtatttacag cacccactaa tttagcttga ggagataaat
    27841 catctagcaa agggataaca ctctgtttaa atggcattga aacattaaca ccacgaatac
    27901 tcaatgccct gacacctcga acagcttctg ttaatttacc ctcttctact tcaaatgtca
    27961 gataggcata attcatgttt ttttcttgaa aagaggtatt ccacattaac ggggatagag
    28021 agtggcgtgc aggattggca attagaccag ttagtaaagt ttccccattt aatgaattca
    28081 ttgtataacc ttctttctta acaatttatt tcagttcatt ttaccaaaaa ctgactcaat
    28141 taaaaagaga gtatgtaatt tatactctct cccttagttt aaggcaatat attacctgca
    28201 ctacggtaaa tatcatacca ttctttacgg gttaaagcga tatcattagc cttatcaatc
    28261 gcctttaaac gagatggatt cattgaacca acaatcgcct gcattttggc aggatgacgt
    28321 aaaatccatg caatcacaat agcttcgctg ctaacattgt agttttttgc cagtttagct
    28381 atggtttcat ttaattcctt ataatccgga tgattaacaa ataaaccacg agataaatca
    28441 atttgaaatg gagaccaagc ttgaatggta actcttttaa gacgacaata atcaacaatg
    28501 ccatcatcat gttcaattga agctttattt aacatgttaa catttaaccc tgcatcaaac
    28561 attggtgtat gtgctggcga taactgaagt tgattcactg ctaagggctc gtcaaggtag
    28621 gattgtaaca actccatttg aaaccggttt tgattgctaa caccaaagtg ttttacttta
    28681 ccttctgccc tcagtttggt aaatgcttct gctacttctt caggctcaac taatacatct
    28741 ggacgatgaa gaatgagaaa atctaaatat tctgtcccta atctttctaa aataccatct
    28801 actgatgata aaatatactc tttagaaaaa tcaaagtaac cttctcgtat cccacattta
    28861 gattgaataa tcattttgtc acgattgaca tttaaatgtt tgattgcatc tcgaaagcgt
    28921 aattcagata gccctccccc ataaatatca gcatgatcaa aaaaattaat acccaaatct
    28981 aatgctgttc caactacttc ttcagcttgt tttcctttca agtcactcat tctcatacaa
    29041 cctaaagcaa ttcgagttgc ttgaatgcct gtttgtccaa tgttttgtaa catatgcacc
    29101 ccattctaat ctgtttgtat taacagaaag attaatcaat tcattataac aaaatttcta
    29161 tgaaaaggct tactattaca aatgcaaaaa gctgagtaaa tttaactcaa ctttttttct
    29221 gatactcatt attgactaaa taagtcggta agaaaaatct attctgaaaa gtactattat
    29281 aaattacagt aatcattatt gtcataacat acctccacat ctgtgatagg aatatcatat
    29341 gacagcaagc ttaaactgag cttagcacag ccttaataga aacttaatca aaatctgaat
    29401 gttggcgtaa attatctaga ataagtgccc attctgtatt ttctttccaa ttattatctt
    29461 tgacaatatc actagcaaca cgtctggctt cttctaaaat aggataatcc tcaacgatat
    29521 cagctacttg aaattctgga attccagact gacgagtacc aaagatttca ccagaaccac
    29581 gcatttttaa atccgactca gctaaaacga aaccatctgt cgtttctgtc atgattgtca
    29641 ttcgtttttt cccagaatca gttttgggat tagccactaa aacagcgtat gattgtttat
    29701 atccacgacc aacacgccca cgaagttgat gtagctggct taatccaaaa cgatcggcat
    29761 ccataataat catgatcgtt gcatttggga catttacccc tacttcaata actgttgtag
    29821 atactaaaat atgacttttt ttatccttga aatcttgcat tatagcatct ttttcatcat
    29881 ttttcatacg tccatgtaca agagcaacct tagcaattcc ttcaaaataa gtagataatt
    29941 cagcatgcaa tgctactgca ttctttaaat ctaaagcttc tgactcttca atcaacggtg
    30001 aaatgacata cacttgagca tctttttgca attcaccttt tacccattcc aacacagtac
    30061 ctagctgctc gtgtttcacc cagcgtgtaa taataggttt acgaccggca ggtaattcat
    30121 caataataga aacatccatt tctccaaaag ctgtgattgc tagagttcgg ggaattgggg
    30181 tggctgtcat cattaaaaca tcaggatttt ctcccttttc acggaaaatt ctacgctgtt
    30241 taacaccaaa acgatgttgc tcgtctgtaa ttacaagccc cagtttatgg tactgtaccg
    30301 agtcctggat aagagcatga gttcctacaa tcatatcaac cgagccattt gcaatagctg
    30361 ctaaaaccgt acgcttaaca gctgctttca tacccgaagt taatatagcg attgataaat
    30421 ctggaaataa ctcttgcaga ctaatgtagt gttgttcagc taggatttcc gttggaacca
    30481 tcaaagccga ttgaaaacct gctgtataag ctgcatacat tgatagacca gcaatgactg
    30541 tctttcctga tcctacatct ccttgcaata aacgattcat atgagctccc gatgacatat
    30601 cagataatat ttcatctaaa gagcgctttt gagcattcgt tagaataaaa ggtaaagatg
    30661 aaatctttgt ctccatagca tgtttactat aaagaataga caaaccattt gtttcagatt
    30721 tattttcggc tttcaaaact tgaaggttca tttgaaagta aaataattct tcaaatttaa
    30781 tccgacggag cgcttgctta tactctgtga tatcttttgg gaaatgcata gctaaacaag
    30841 cctgactacg acccatcaat cggtattttc ccaataaagt agctggtaaa ttttccttca
    30901 attccaaatg cgcattgatt tcaaaagctg acttaatagc tttaatcaaa gtagactgtg
    30961 aaattccctg agatacgcga taaacaggtt gcatgtcatc ttcaacttga gctaaaacct
    31021 tcatcccagt aatagctgat ttagttgcat cccatttacc aaagacagct acctcttgac
    31081 caagttctat tttatcagct aaatagggtt gattaaaaaa actaacattt aagacagctt
    31141 ccccttgacg caatttgaaa cttaaacggt tacgtttaaa accataatat tgtacattag
    31201 ctggagtaac gactaagcct gtaatgactg ctttttcacc atccactaaa tcaaaaacag
    31261 attttgattt aaaatcttca tagcgaaacg gataataaag aagtaaatct tctactgtat
    31321 aaatatctaa tttctgaaat ttttctgctg attttggtcc aaatccctta agattagaaa
    31381 ttggtgattg taatagcata agattctcct tctaatatct tttattatac taaaagatca
    31441 ccaaaaaggc gcctcaagag cgcctttatt atgtctaatt atttatggcc aataacataa
    31501 gttaattgtg cagcttgtgc tgaactaaaa gtacggtaag agacaatacc acgtcccatt
    31561 gcattagatt ctgaaatgag aactgaacca tcttttttaa cttcttcaac aatagctacg
    31621 tgaccgtaag tgccatcagc accagcttgt cctggtgaaa atgatacagc atagcctact
    31681 tttggtgaat gtgttgtttc aaagccagcc ttatgttgcc aatctccacc attacccata
    31741 aatggatcaa atgtataacc taactcttta gcgcggttaa agacatacca tgtacattca
    31801 ccccatggat aagttgatgc aatatagcta gctgtgttaa ccgctgttga taagctaaaa
    31861 ccacttggta aatctgctaa acttgctgtc gataaagctt tcaaggcagc aggaatttct
    31921 ggtgtccctc catgttgatc aatccagcgg tctaaatctt ccataatctg ggcacgacgt
    31981 ttaccagttc cagagttatc tgtataataa acaccaccct cagtaactgt attaagttgt
    32041 ccagaagata atttctgtgc tttcaaatct tggatttcaa agctagagtt gttgttttta
    32101 ataattgtat tttttgtcaa caacataatt gcttcttcat cattataagc actagcatgg
    32161 ctagagtcat gatcaaaggc accataacca aacatattag cacctggcat cttagccact
    32221 ccttgagttc ccaatgaact ttcaaggaca gcaatagcta caatagcacg gacatctaaa
    32281 ccactatctt tttcccattg taataactta gcaccattga tacgattaga atcatattga
    32341 atccctaaag attttaagta cccatctaat tgttcagctt tgataccgta acgatgtgac
    32401 aataaatagt gagagtaggc atcctgacca gaccagtgtt ctacgtagaa tggcgttgct
    32461 gtcactggtg aaatcggtgt tgataataag ttatcatcaa aggataaagc aggaacagaa
    32521 gcattctgat ttgtctgaac actttctatc tgagacggag cgggagttat tttaggctct
    32581 actaatggta aggtaagctc aggttttaat tcagtctttg ttgattgacc tggcttactg
    32641 tctgcttgac tactgtttgt agtattagtg ctaggagtag atgctgttga agcagaatca
    32701 gatgttttag catcttcgtt ggaagaagat tgatgtgatg aacttgatga tgattcattg
    32761 cttgatgatg tctctgtcgt accaatctct gttttttctg ttgagatact tccttcggct
    32821 gaaggtgaag cagtttctga atctgtaggt tgtgtttttt cctgtttctc tgcttcagaa
    32881 gaactttctg ttgatgctgt tgccattgtc aggcttgttt ctggattaat agtttctgct
    32941 aaggtcacta aaggtgatga tagcgataag actaaagtgg ataacaacaa agttgccgtt
    33001 actttttgtt ttgacatata ctaatgattc ccttctataa aaattgtcac tgattagtct
    33061 aacatatttt taaatttttt aaaacagtta actactgtgt catttgtttt taataatatc
    33121 tcggaatacg atcgcttaaa agacaaagta cttcataatt aatcgtctgt cttttttgag
    33181 cgacgtccgt agttgtaatg ttacaactac cttgttgacc aatcaaggtt actttagttc
    33241 cgatagtata tttttgaggt aaacggatag tcatttgatc catcgagaca cgcccaataa
    33301 tttcacaaag ttctccattt actataactg aaaatccttg catatctcgt gtccatccat
    33361 cagcgtagcc aatcggtact gttccaacaa attcgtctcc agtaacttga taggttgcgc
    33421 catagccaac ttgagaacca tcgtgtagtt gtttaacatg tactagctca gactctaaac
    33481 ttaaagcagg gtttataggg taaggtaagt ctaaatcagt tccacttgga tttaagccgt
    33541 acataacaac acccaatcta acggcattaa aaattgtctc actatgccaa aggcttgtag
    33601 cagaattact agcatgaaca aggtccggac aattatctaa tcctgaaata aagtctttaa
    33661 aaaaagtaag ttgttgattg aatttacagt tatcggcttc atcagctgtt gcaaaatggg
    33721 tgaaaatccc cttgacagag gcaccacttt cccccaattc agaaattagc ttatttcctt
    33781 catctaactg gcgaacacct atacgtccca ttccactatc aactttgata tgtacttcta
    33841 gaccagataa atctactgct gatgtctgac ataaacgtaa ccactccaag ctagtaactg
    33901 ttagagtaat attttcattt ttggctagta taacctgctc aggcataaca acacctaata
    33961 ccaatatcat ttttacaatt cctgcttgac gtagctcaat agcctcatct aaattggata
    34021 cgcaaaaacc atctacaata gactcaatgt attttgaaac ttcaacagca ccatgcccat
    34081 aagcattagc cttaactaca gcaaatgttt ttttatcact tggaatgtgc tcttgtacag
    34141 atttaacgtt atttgcaatc gcctctaaat ctatcaaggc tctggttggt ctatgataac
    34201 ttgaaatcat ttttcttcct ccaaaataac actagcttgt gcataattac cactatgaga
    34261 tattgaaaca aaagattttc cattaaacgg ttcttttgta atcaaaggcg ctcctttatc
    34321 atccgataaa atttcgatat catgaaaatt aactttccca attcctgttc caagtgcttt
    34381 gctataagcc tcttttgctg cccatcgccc tgttaaaaaa gacatctgac gcttgggatt
    34441 ggaaattcct ttaaaaagaa gcaattcttg ttcggtcaaa acgcgttctg caaaacgttg
    34501 attacgctca tatgctttag taatcgcctc tatctcttgt aaatcaattc cgtgtccaac
    34561 aatcatgtgt aactcctata agttcgatac ttacaaacta ttgtaaatat ccctaattag
    34621 catttctgtt ttatcccaac ctagacatgg atctgtgata gacttaccaa aaacatctgg
    34681 tttatcttgt cttccatctt ctaaataaga ttctatcaaa aaaccacgaa catagtttct
    34741 aattttagta tgccactgtc ggtctgcaag tactgatttc acaatgcgaa tttgttctaa
    34801 aaattgctta cctgaattgt catgatttgt atcaacaaca ataaagggat tttgcaaatc
    34861 agtctcttca taatgagtaa tagtttctaa aaggttttca taatgataat tagggatact
    34921 tcgatagtcg gcattatgat atcctctcaa aataacatgt gacagcaaat taccatctgt
    34981 tctaacttgc ttattttgat agaataattc ctgttgattt tgtgctgcat agaccgcatt
    35041 aaacatgact cttaaattac cagacgttgg atttttcata cctactggtg catctatacc
    35101 tgaagatata aaacggtgtc cctgatcttc cactgaacgc gcaccaatag catagtaaga
    35161 aactaaatca tccatcactg ataacatctc tggataaagt aattcatcag ctgtcaggag
    35221 tccagtctct cgaatgatgt catagtgcat cttacgtgct tgaaaaaaaa ctcctagttt
    35281 gtcagaatgg tgaactaagc ctttataacc atcaccattc gttcttggct tagcggtata
    35341 aactctcata accaaaaaca ttttatcttt aaaggcagct tccaatttag ctaaacgctt
    35401 tgcataagtt agcaccgcct ttggattatc cgcagaacaa ggacctacta ttactagaaa
    35461 ccgctgatcc tcacctttta tgatattttt taactcttct tctttggcta attttttttg
    35521 gctaacttct agatctaagc tagttttctg cttcaatatt tcaatattaa ttttatcact
    35581 taactgatga aatcccatgt tttttcctaa tacaacctaa gtctattaat aagctctgct
    35641 atacaatata gtcgtcatac tattagacaa gtaatgataa aacagtcttc tcagactgtt
    35701 ttatttttat tttaaaatca ttgttattgt aaacatctca gaccatgaca atttttaaac
    35761 ttcatgccag agccacaagg acatttatca ttacgtttaa tcccttggta ttctggtgat
    35821 tcattattca taggtacatg ttgtgccgag atattttgct ctgctgttgt cgtagcatgt
    35881 tgagaagcac gttcacgctc ttgttcatga atttgagctt tcatcaatgt acgtgtcaca
    35941 tcaaactcaa tcgaaccaat catatcttgg aacatacgga aaccttctga ttgatactca
    36001 acgattgggt tattttgagc ataaccacga agtccaacgg agttacgtaa ttgatctaag
    36061 gcatcaatat gctctgtcca ttgattatcc actaccatca agattaagac tttttggaat
    36121 tcgataatag cttctggatc ttttaattta gcaatttgtt gctcataaac agctaaggca
    36181 cgttcataaa gaagttcttt aatttcagcc tcttttagac cacgaagttc tgaaactgtg
    36241 atagcttctt catctaataa cgccgatctt gcaaaattaa caatagcttc tgctgcagta
    36301 tttttatcgc ttctgctatg tgcatcaact gctcttttaa tcgtacgttt aatcatccct
    36361 ttgagctctg atcctaaatc acgctcagca gtaatcactt ctctacggtt agcataaata
    36421 atctcacgtt gttcacgcat aacatcatcg tattgcaaga cttgtttacg tgtgtcatag
    36481 ttattacctt caacgcggcg ttgagctgat tcaacttgtc gtgtcaacat ttttgattta
    36541 ataactgtat catcttcagc taaattcatt cgctcaagaa ccactttaat acgatctgta
    36601 ccaaagcgac gcatcaaatc atcttctaac gaaagataga attgtgattc acctggatct
    36661 ccttgacgac ccgaacgacc acgaagctgg ttatcaatac gacggctttc atgacgctct
    36721 gtaccaataa cacataaacc accaagttca cgaacccctt caccaagctt gatatcagta
    36781 ccacgaccag ccatgttggt tgcgattgta acagcaccac gttgcccagc attcataatg
    36841 atttgagctt ctttaaagtg atttttagca ttcaaaactt catgtggtac tccagcagca
    36901 actaattttc tagaaattaa gtctgacgtt tcaacagcta ctgtaccaac cagtacaggt
    36961 tgtccttgtt cataacgttc tttgacatct gctaccaccg ctctaaattt agagtcaaga
    37021 gttggataaa gtagatctga atgatctata cgttgaacag gacgatttgt tgggatagga
    37081 ataacgcgca tattataaat ttcacgaaac tcttcttcct cagtcttacc agtaccagtc
    37141 attcctgcta atttatggta catacggaac atattttggt aagtaattga agcgctcgtt
    37201 tttgattctt cttggattgg cactgactct tttgcttcaa tagcttggtg taagccatct
    37261 gagaaacgac gtccttccat cgtacgaccg gtaaattggt ccacaattaa aatctcttgt
    37321 tcttcactaa ccacataatc aatatttaac aacataatat agttagctcg aagagcgtta
    37381 tcaatgtagt gagttaaggc aacattttcg agatcataca agttatttag atggaaatag
    37441 ttttcagcct tgtcaatacc tgtatctgat aaaccaattg tttttgttgg aacatcaatg
    37501 atataatcat cgctattaag cgtttttaca aacatgtccg cacgtgtata cagttggttc
    37561 atctcagagc ttacaggacc tgaaacaatc aatggcgtcc tagcttcatc gattaataca
    37621 gagtcaacct catcaaccaa agcatagttg agtgggcgtt gcaccatatc ttcttggcga
    37681 acaaccatat tgtctcgaag gtagtcaaat ccgacttctg cgttagttga gtatgtgata
    37741 tcacaattgt aagcttcgcg tttttcaaat ggtgacttag ctgctaagtt aatccctaca
    37801 gataatccaa gccaactata aacctcaccc atttcggtcg catcacgtgt tgataagtac
    37861 tcattaacag taataacatg aacacctaat cctgagatag catttagata aacaggcatt
    37921 gtcgctgtta aagtttttcc ctcaccagta cgcatttctg gaatatcacc atggtgaaga
    37981 acgataccac ccataatttg gacgtggtat gggtataaac ctaatacacg tttagaagct
    38041 tcacgaacta ccgcaaacgc ttccggtaac aattgatcta aagtttcacc attttgataa
    38101 cgttctttaa attctggagt ttttgcttgt aaagcttcat ctgataacgt agccatatga
    38161 tctgcgtaag agtcaacttt cttagctatt ttatctaatt tttttaattc acccttgtca
    38221 ttttcaataa cagtgcgtag aatatttgcc attctaattc ctttcgatac tcttatcacc
    38281 acattttacc ataaataatg ttgtctttca ataaatggtc gttttaatcg agattaaaac
    38341 aagtggtatc cccactctca ttatttttta acatgacttg ctataatttc taactgtcca
    38401 tcaatatccc atttgacaac atcatttggc aagataaagt ggtcaccttt tttaagtgta
    38461 taaactttgt tatctactgt aatgtgaccc gctccgtcta agacactaac caaaaggtag
    38521 ggtgcaaatt gtttaaagtt ggtaacacca gaaatttccc atttataaac tgtaaagaaa
    38581 tcatttgaaa ctaataaagt tgaagataga tgttttaact tcatattggc aggaacagta
    38641 ttggcaggtt taccaatcgt gagaacatct attgattgtt caatgcgtaa atcacgtaat
    38701 tttcctgaag catctggacg atcaaaatca taaacacgat aagtagtatc actagattgc
    38761 tgtgtttcta aaattaatat ccctttacct atagcatgca ttgtcccact tggaacgtag
    38821 taaaaatccc ctgatttaac aggaattcgg gttaaaagat gttcccaatc tccagattca
    38881 atcatttggc gaagttcttc tttagtttta gcattatgac catagataat ttcagatcct
    38941 tcatcagcag aaataatata ccaacattct gttttaccaa gttcaccttc atgtttaaga
    39001 gcataagcat catccggatg aacctgtaca ctaagccagt cattagcatc taagatttta
    39061 gttagtaaag gaaagacagt atcatcaggg ttgccaaata agcttttttc accttgatac
    39121 agcttatcta aaaaacaacc tttaaagcga ccattcttaa ctcttgagac accgttagga
    39181 tgagctgaaa tagcccaata ttctcctgtt gtttcacttg gaatatcata gccaaattca
    39241 tcccttaact ttgttccacc ccagatttta tcgtgcatgc ttgcttctaa aaataatggt
    39301 tccgacataa gaatccccta tcttttgtaa aattcaagcc tattatagca ttttatccat
    39361 catatcgctt acttttttta atcttttatt tgctaactaa actaagtagt ggcttaaggg
    39421 ttttattaac cttttgatat tttctttgct aaagcaaaat ttcctaacgt tgcagaacca
    39481 ttttcttcta tagctggtgt cacaatataa tcagacaaat cgggaactgg taaataccca
    39541 tttaataaag ttgcaaatgt ctggcgaaca cgtcttaaca tgtgctcttg tgccatgacg
    39601 cctccaccaa atacaatgac ttgtggacga tataatacag ttgcttgtat tgcagcctga
    39661 gcaatataat aggcttgaac atcccaaacc attgaatttt cttcaatcaa ttcaccacga
    39721 ataccagtcc tagcttctag agttggacca gatgccaaac cttctaaaca gctattatga
    39781 aacggacaga tacctttaaa atcatttgct tggtcctgcg gatgcatcgc catataagta
    39841 tgacctgctt cagtatgacc agttcctcct ataaattcac ccttttgtat agctcctgca
    39901 ccaattcccg taccaatggt gtaataaaca agtgaatcaa tattgttacg agctataact
    39961 tcaccatagg cagagctatt aacatctgtt gtgaaataaa atggaacatt gaaatcttta
    40021 gaaattaagc cgagtaaatc aacattagcc caatgtaact ttggggtcgt tgtgatataa
    40081 ccgtatgtct ttgatttttt atcaatatca atgggaccaa aagaaccaat agcaactgcc
    40141 tctaacttct tttcaaatct tttaaagaaa tcaacagtct ttttaatcgt ttcttgaggc
    40201 gtcgttgttg gaaattgcat tttttctaca acttttaatt cctcatcacc aactgcacaa
    40261 acaaactttg tcccacctgc ttcaatacta ccatataact ttgtcatctt ttttcctatc
    40321 tataatctct atctcaaaga caaaggttgg gataattatc ccaaccttta atcctcactt
    40381 aacatcaata atcatgtcac ctgtagtaac aacaccctct tttaccaatg gtataacatc
    40441 agcaaaatca gctgtattag tcacaataat catagctgta ttatccagac ctgcttctgc
    40501 aatcttggct gaatcaaagg tgccaagaat atcacctttt ttcacttttt ggtctgcctt
    40561 gactttagaa gtaaaccctg tgccattcat ggtgacagta tcaataccta tatgaatcaa
    40621 aatttctgca cctttatctg atttgatacc gtaagcatga cctgtttcaa aagcaatttg
    40681 gacaaaacca tcaacaggtg agtaaattgt attacctctt ggtttaatag caaggccatc
    40741 tcccataata cctgatgaaa aaactgggtc acttactgta tcaagagcaa taacctgtcc
    40801 atcaagaggg ctaacaatcg tttctgccct taattggcta gtttgagtaa ttgttggatc
    40861 gtcatctgtt tcttcaacca aaggactaac ttcagctact gctttttcat ctttatcttc
    40921 atatccaaac atataagtta atacaaaagc caaagacgta gttccaatta ccataataag
    40981 gtacttaaca atttgaccat ttaaataaag taatgttcct gggataatgg taataccaaa
    41041 accagttcct gctaatttca atattgaagc taaccagcca cctgctgctc ctgcaattaa
    41101 tcccataata aatggcttac caaaacgtaa gtttactcca aatatagccg gttctgtaat
    41161 accaagtcca gctgaaagcg ctgccggaaa agcaagggct tttaattttt tattttttgt
    41221 tttaacacca actgccaacg tggcacctgc ttgtgctgtc atagcagctg taataatagc
    41281 attaaatgga tctttcccat cagcagcaat taactgagct tcaagtaaat taaagatatg
    41341 atgaacacct gtaacaacga ttatctgatg aacaccacct aaaataagac ctgataggcc
    41401 aagaggtaaa ttaaggacaa attttgtccc tgctaaaaca tagttttcaa ctgaatggaa
    41461 aataggacca atgacaaaaa gtgctaaaat tgacataact gtaaaagtta ggaacggcac
    41521 taatagtaaa tcaagaacat ctgggatttt cttatgcaac catttttcaa gtttagcacc
    41581 aagtaaacct acaaagaatg ccggcaatac tgaattttga taaccaacta ctggaataaa
    41641 gccgaaaaat tttatgggat gggcatcacc tgaggccact gcccaagcgt ttggaagggc
    41701 agagttgacc atcatcaatc ctaatacgag accgataaca ggatttccac caaaaactct
    41761 aaaagctgac caagaaatca aggcaggaaa aaaagcaaag gccgtgtcag tcaaaacgac
    41821 agtataggta taaaaattag aacttgaaaa tgcttttgaa gtcgtcccaa ataaagctaa
    41881 aactgtatca ttattaatcg caccgcgaat ccccataaat aaaccagtag caacaatggc
    41941 tggcaacaat ggaacaaaaa catcaccgaa agtacgaata gctcgttgga aagcattccc
    42001 ttgtttagcc gcttctgcct tttgatcaga ggttgacaaa gttggcaaac cttgagccac
    42061 tacttcatca taaatcttat taacagtacc agttccaaaa ataatttgat attgacctga
    42121 attgaaaaat gctccctgaa ctttttcgat attttcaaca gtatttttat caatgactgt
    42181 ctcatctttt accataatgc gaagacgagt ggcacagtgt gctacgctac gcacattact
    42241 agcaccacca atcgcattaa tgacttgttt agcaatttct gtatttgaag ccatctataa
    42301 atctcctttg acattttgaa agcgtttcat tttatttact ctaatattct agcatgtctc
    42361 ataaatatat gtcaagcgtt tgacataaaa taatattttt tg
//
LOCUS       DATRNX010000005        32833 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRNX010000005 DATRNX010000000
VERSION     DATRNX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32833
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            181..381
                     /locus_tag="VBC81_000230"
     CDS             181..381
                     /locus_tag="VBC81_000230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6911298.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            413..1129
                     /gene="atpB"
                     /locus_tag="VBC81_000231"
     CDS             413..1129
                     /gene="atpB"
                     /locus_tag="VBC81_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6911299.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            1147..1644
                     /gene="atpF"
                     /locus_tag="VBC81_000232"
     CDS             1147..1644
                     /gene="atpF"
                     /locus_tag="VBC81_000232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6911300.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            1644..2180
                     /locus_tag="VBC81_000233"
     CDS             1644..2180
                     /locus_tag="VBC81_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6911301.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            2196..3701
                     /locus_tag="VBC81_000234"
     CDS             2196..3701
                     /locus_tag="VBC81_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6911302.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            3717..4598
                     /locus_tag="VBC81_000235"
     CDS             3717..4598
                     /locus_tag="VBC81_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6911303.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            4672..6078
                     /gene="atpD"
                     /locus_tag="VBC81_000236"
     CDS             4672..6078
                     /gene="atpD"
                     /locus_tag="VBC81_000236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6911304.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            6091..6504
                     /locus_tag="VBC81_000237"
     CDS             6091..6504
                     /locus_tag="VBC81_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6911305.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            6569..6799
                     /locus_tag="VBC81_000238"
     CDS             6569..6799
                     /locus_tag="VBC81_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6911306.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            6862..8133
                     /gene="murA"
                     /locus_tag="VBC81_000239"
     CDS             6862..8133
                     /gene="murA"
                     /locus_tag="VBC81_000239"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6911307.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            8135..8326
                     /locus_tag="VBC81_000240"
     CDS             8135..8326
                     /locus_tag="VBC81_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6911308.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            8401..9258
                     /locus_tag="VBC81_000241"
     CDS             8401..9258
                     /locus_tag="VBC81_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6911309.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            9549..10589
                     /gene="pheS"
                     /locus_tag="VBC81_000242"
     CDS             9549..10589
                     /gene="pheS"
                     /locus_tag="VBC81_000242"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6911310.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            10672..11192
                     /locus_tag="VBC81_000243"
                     /pseudo
     CDS             10672..11192
                     /locus_tag="VBC81_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11246..13651
                     /locus_tag="VBC81_000244"
     CDS             11246..13651
                     /locus_tag="VBC81_000244"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6911311.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(13720..14622)
                     /locus_tag="VBC81_000245"
     CDS             complement(13720..14622)
                     /locus_tag="VBC81_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6911312.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            14800..18033
                     /gene="rexB"
                     /locus_tag="VBC81_000246"
     CDS             14800..18033
                     /gene="rexB"
                     /locus_tag="VBC81_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6911313.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            18023..21646
                     /gene="addA"
                     /locus_tag="VBC81_000247"
     CDS             18023..21646
                     /gene="addA"
                     /locus_tag="VBC81_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO6911314.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            21659..22576
                     /locus_tag="VBC81_000248"
     CDS             21659..22576
                     /locus_tag="VBC81_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6911315.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(22608..23984)
                     /gene="mnmE"
                     /locus_tag="VBC81_000249"
     CDS             complement(22608..23984)
                     /gene="mnmE"
                     /locus_tag="VBC81_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6911316.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARHG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            24112..26022
                     /locus_tag="VBC81_000250"
     CDS             24112..26022
                     /locus_tag="VBC81_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6911317.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            26172..27140
                     /locus_tag="VBC81_000251"
     CDS             26172..27140
                     /locus_tag="VBC81_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6911318.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            27215..28213
                     /locus_tag="VBC81_000252"
     CDS             27215..28213
                     /locus_tag="VBC81_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6911319.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            28340..29728
                     /locus_tag="VBC81_000253"
     CDS             28340..29728
                     /locus_tag="VBC81_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6911320.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            29788..31545
                     /gene="lpdA"
                     /locus_tag="VBC81_000254"
     CDS             29788..31545
                     /gene="lpdA"
                     /locus_tag="VBC81_000254"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6911321.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            31643..32632
                     /locus_tag="VBC81_000255"
     CDS             31643..32632
                     /locus_tag="VBC81_000255"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6911322.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT        10755 a         5298 c         6646 g        10134 t
ORIGIN      
        1 catatcttaa taagggatta aattaaagct aaaaaaacta aaaattgtta tttgaaaagg
       61 attgtgtaaa ccatctcaaa ctatttcatg aaaacgtaat caaatgagga aattcattga
      121 catgcacaag aaataggaat aaaataacgg agtaaatatt ttttaaagga gaattctatt
      181 atgaatttag ctattttagc acttggtttc gccgtgatgg gtgtttcaat cggtgaagga
      241 attttggttg caaatattgc caaatcagcg gctcgtcagc ctgaaatgtt tagcaaactg
      301 caaacattga tgtttacagg tgtggccttc attgaaggta ccttcttcgt tctatttgcg
      361 tttacattct tagtaagata gacgacaatt tagaaaaaag gagggatgag gattggaatc
      421 tacaagtaat ccaaccgtat cttttttagg catcgatttt gatttaacca tcctagctat
      481 gtctctcttg acaattacta ttatctttat cttagtcttc tgggctagtc gtaagatgac
      541 aataaaacca aaaggtaagc agaatgtctt agaatatgtc tatgagcttg ttaataatac
      601 aatctcacaa aatttaggac attatactaa aaattatagt ttattgatgt ttattttatt
      661 cagttttgtc ttcattgcta acaacttagg attgatgaca tcattgaaaa cacatgagca
      721 taatttttgg acatctccga ccgctaattt tggtgtagat attacattat cattattagt
      781 agcatttatt tgtcatattg aaggtattcg caaaaaagga attggtggtt atctcaaagg
      841 tttcttatca ccaacaccag caatgcttcc gatgaattta ttggaagaag ttactaatgt
      901 tgcttcatta gcattgcgtc tttttggtaa tatcttttct ggtgaagttg tcactggatt
      961 acttttacaa cttgcagttt tatcaccatt tacaggtcca ctagcttttg cattgaatat
     1021 cgtttggaca gcattttcaa tgtttattgg tttcatccaa gcgtatgtgt tcattatctt
     1081 gagctcaagt tatattggac acaaagttca tggtgacgaa gaagaataag aaaagagagg
     1141 agaaatatgt caatacttat taatagtaca acgattggtg atatcattat cgtatctggc
     1201 tctgtgcttc tcttatttat cttaattaag acttttgcat ggaaacaaat cacaggtatt
     1261 tttgaagccc gtgaacaaaa gatagctaat gatattgata ctgctgaaca ggcacgtcaa
     1321 caagctgaag cttttgcaac taagcgtgag gaagaactat ctaacgctaa aactgaagct
     1381 aatcaaatca ttgataatgc taaagagaca ggtcttgcaa aaggtgatca aattatttca
     1441 gaagcaaaaa ctgaagctga tcgattaaaa gaaaaagcac atcaagatat tgcccaaaat
     1501 aaggcagaag cactagcaga tgttaagggt gaagtagcag atttaacagt tttattagct
     1561 gaaaaaatta tggtatcaaa tcttgataaa gaagctcaga gtaatctcat tgatagctat
     1621 attaaaaagc taggagatgc ctaatgaata aaaagacgca ggctctaatt gagcaatatt
     1681 caaaaagcct tgttgaagtt gccattgaac ataaaattgt tgaagaaatc caacaagaag
     1741 tagcagcttt aattgatatt tttgaaacaa gcgagcttga gggtgtcctg tcaagtcttg
     1801 ccgtttcaca tgatgaaaaa caacattttg tgaagacatt gcaaacatca tgttcaactt
     1861 atcttgttaa tttccttgag gttattgtac aaaatgaacg tgaagcactt ctatatccta
     1921 ttttgaaatc agttgatcag gaacttatca aagtgaatgg tcaatatcca attcaaatta
     1981 caactgcagt tgctttgtct ccagaacaaa aagaacgtct ttttgatatt gctaaaacta
     2041 aattagcttt accaaatgga cagttagttg agcacattga cccatctatt gttggtggat
     2101 ttgttgtcaa tgcaaacaat aaagtaattg atgcaagtgt tcgtaatcaa ttacatcaat
     2161 ttaaaatgaa attaaaatag aaagtggtgt gacttttggc aattaatgca caagaaatta
     2221 gcgctttaat taaaaagcaa attgaagact tccagccaaa ttttgacgtc acagaaactg
     2281 gtattgttac atatatcggt gatggtattg ctcgtgctcg tgggctagat aatgctatga
     2341 gtggagaact tcttgaattt tcaaatggtg cgtacggtat ggctcaaaac cttgagtcta
     2401 atgacgtggg tatcattatt ttgggtgatt tctcagaaat tcgtgaaggt gatgtggtaa
     2461 aacgtactgg taaaattatg gaagtaccag taggggaagc tatgattgga cgtgtcgtca
     2521 atccgcttgg tcaaccagtt gatggactag gtgaaattga gacgacagca acacgcccag
     2581 ttgaaacacc agcacctggt gttatgcaac gtaaatcagt ttttgagcct cttcaaacag
     2641 gtctcaaagc gattgatgct ttggttccta tcggacgtgg gcaacgcgaa ttgattatcg
     2701 gtgaccgcca aactggtaag acgtctgtag cgattgatgc aatcttgaat caaaaaggtc
     2761 aagatatgat ctgtatctat gtggctatcg gtcaaaaaga gtctacagta cgtacacaag
     2821 ttgaaacttt gcgtaaatat ggtgcccttg attacacaat cgttgtgaca gcttcagcat
     2881 cacaaccatc tccattactt ttcattgctc catatgcagg agtagcaatg gctgaggaat
     2941 tcatgtataa cgggaaacac gttttgattg tttatgatga tttatcaaaa caagccgtag
     3001 cttatcgtga actttcgctt cttcttcgcc gtcctccagg tcgcgaagct tacccagggg
     3061 atgttttcta tcttcatagt cgtcttttgg aacgttcagc taaagtatca gatgctttag
     3121 gtggaggttc gattacagcg cttccgttca ttgaaacaca agcaggtgat atctcagcat
     3181 atattgctac aaacgttatt tcaattactg acggccaaat tttcttgcaa gaaaatctct
     3241 ttaactcagg tattcgtccg gcgattgatg caggttcatc agtatctcgt gttggtggtg
     3301 cagcgcaaat taaagcgatg aagagagttg caggtacgct tcgtctagat cttgcttctt
     3361 atcgcgaact tgaggcattc actcaatttg gttcagacct tgatgcagca acacaagcaa
     3421 aactaaatcg tggtcgtcgc acagtggaag tgttaaaaca accattacac aaaccattgc
     3481 cagttgagaa acaagttgtg attttatatg ctctaacaca tggattcctt gatgatgtac
     3541 ctgtaaatga tattttagct tttgaagaag ctttgtatga ttactttgat gctcattatg
     3601 ataatctctt cgaaacaatc cgcacaacaa aagatttacc agaagaagca gaattagatg
     3661 ctgctattca agcatttaaa gatcaatcac aatttaaata ataggagggt tctaatatgg
     3721 caggctctct tagtgaaatt aaagacaaga ttctttctac agaaaaaaca agtaagatta
     3781 ctagtgcgat gcagatggtc tcatctgcca agttggttaa atctgaacaa gctgctagag
     3841 attttcaagt gtatgcctct aaaattcgtc aaattactac taacctcttg aagtcagact
     3901 tagtatctgg atcagataac cctatgttag cttcacgtcc ggtgaagaag acaggttata
     3961 ttgttattac ttctgataag ggtttagttg gtggttacaa ttcaaaaatc ttaaaagcga
     4021 tgatggacac tataactgac tatcatactg agaatgatga ttatgccatt atttcaattg
     4081 gtagtgttgg ttctgatttt ttcaaagcac gtggaatgaa tgtttctttc gaattacgtg
     4141 gtttagagga ccaaccaagt tttgatcaag ttggtaaaat tattgctcaa gcagttgaaa
     4201 tgtacaaaaa tgagttgttc gatgaattat atgtttgcta caatcaccat gttaacagtt
     4261 taactagcca agtcagaatg caacaaatgc ttcctatcaa agaattggat gcagaagagg
     4321 cttctgagga tagagtgatt actggttttg aacttgagcc aaaccgtgaa gttatattgg
     4381 agcaattatt gcctcaatat actgagagtt tgatttatgg agctattatt gatgctaaaa
     4441 cagcagagca tgcggctggt atgacagcta tgcagactgc aaccgataat gctaaaaatg
     4501 ttatcaatga tttaactatt caatataacc gtgctcgtca agcagctatc acacaggaaa
     4561 ttacagaaat tgttgcaggt gctaatgcac ttgaataatt aattagtcta tagaaaaaat
     4621 agtgaacaaa ggtattttaa atcctttgta tcatatgata aggagaaata catgagctca
     4681 ggcaaaattg ctcaggtagt tggacctgtc gttgacgttg tatttgcaag tggcgacaaa
     4741 cttcctgaga ttaataatgc attgattgtc tataaaaatg gcgataagtc acaaaaagta
     4801 gtacttgaag ttgctcttga acttggtgac ggcctcgttc gtacaatagc tatggaatca
     4861 actgatgggc ttacacgtgg tttggaagta ttagatactg gtcgcgcaat tagtgtgccg
     4921 gttggtaagg atactttggg tcgtgtcttc aacgttcttg gagatgctat tgaccttgaa
     4981 gagccttttg cagaagatgc agaacgtcaa ccaatccata aaaaagcacc atcgtttgat
     5041 gaattatcaa catcatcaga aatcttagaa acaggtatta aagttattga cttattagca
     5101 ccttacttaa aaggtggtaa agtcgggctt ttcggtggtg cgggtgttgg taaaaccgtt
     5161 cttattcaag aattaatcca caacatcgcc caagaacatg gtggtatttc agtatttact
     5221 ggtgtaggag aacgtactcg tgaagggaat gacctttatt gggaaatgaa agaatctggc
     5281 gttattgaaa aaacggctat ggtctttggt caaatgaatg aaccaccagg agcacgtatg
     5341 cgtgtggcac ttactggtct tacaatagct gaatacttcc gtgatgtaga aggacaagat
     5401 gtgcttctct tcattgataa tatcttccgt ttcacacaag cggggtcaga agtgtcagcg
     5461 cttttaggtc gtatgccttc agccgttggt tatcaaccaa cacttgctac agaaatggga
     5521 caattacaag agcgtatcac ttcaactaaa aaaggttctg ttacatcaat ccaagctatt
     5581 tatgtgccag cagatgacta tactgaccct gcgccagcta cagcctttgc tcacttagat
     5641 tcaacaacta accttgaacg taaattgaca caaatgggga tttatccagc cgttgaccca
     5701 ttagcttcaa gttctcgtgc actcacacca gaaatcgttg gagatgagca ctatgaagtt
     5761 gcaacagaag tacagcgtgt tcttcaacgt taccgtgagt tacaagatat cattgcgatt
     5821 ttaggtatgg atgaattatc agatgaggaa aaaacgttag ttggacgtgc acgtcgtatc
     5881 caattcttcc tttctcaaaa cttcaatgtt gcagaaacat ttactggtca accaggttca
     5941 tatgttccgg ttgaagaaac tgttcgcgga ttcaaagaaa tcctagatgg taaacatgat
     6001 caaattccag aagatgcctt ccgtatggtt ggtggtattg aggatgttat cgcaaaagca
     6061 gaaaaaatga actattaatg aggtgatgct atggcacaat tgactgttca agtagtaaca
     6121 tcagatggta ttagatatga ccatcacgct agtctcatta ctgttagaac gccagatggt
     6181 gaaatgggaa ttttaccagg tcacataaat ttgattgctc cccttattgt tcatcagatg
     6241 aagattaatc gttctcatca agaaggtgta gactgggtag ctgttaatgg tggtataatt
     6301 gaagttaatg aggatcaggt gacaattgtt gcggattcag cagaacgagc acgtgatata
     6361 gaccttaacc gtgcagaacg ggctaaggaa cgcgctgagc gtgcattaga aaaagctcaa
     6421 acaactcaga atattgatga gatgcgtaga gctgaggttg ctcttcgtcg cgcaattaac
     6481 cgtattagcg tcggtaaaaa gtagaaaaat ggtctaatta gttttgtgac taattaggct
     6541 tttttagtgt ataatagaag gcatgaatat gatattaaat atagtaacta tagttagtaa
     6601 ttttattttt attgcccttt tttatcaact ttttgtagat ttatttgact ggtctaaaat
     6661 gattaaaatg tcccctcaaa acatctcaaa attaaaagtt tttattttgt tgataagtat
     6721 agttggtggc tatgtggtta gtcatttttt attggaagtg attcagttgt gtcaatcaat
     6781 cttttgggct ctccaataaa ggaaatatta gctgaactat gatataatca acttactatt
     6841 gttataagaa tggagattca aatggataaa attattgtgg aaggtgggca aacgcggctt
     6901 caaggtcaag ttgttattga aggagctaaa aatgcagttt tacctctttt agcagccaca
     6961 attttaccaa gccaagggaa aacattattg accaatgttc ctatcttgtc tgatgtattt
     7021 acaatgaaca atgttgttcg tggtttagat attcaagttg actttaattg cgataaaaaa
     7081 gaaatcttag tggatgcctc aggtgatatt ttagatgttg caccttatga atttgttagt
     7141 caaatgagag cttctattgt tgttttggga cctattttag ctagaaatgg ccatgctaag
     7201 gtttcaatgc caggcggttg tacaattggt agccgaccaa ttgaccttca tcttaaaggt
     7261 ttagaagcta tgggagctac gattacacaa aatggtggtg atattactgc ccaagctgaa
     7321 aagcttaagg gggctaatat atacatggat tttccctcag tcggtgccac tcaaaacttg
     7381 atgatggctg ctacacttgc ttcaggcaca acaacgatag agaatgctgc acgtgaacca
     7441 gaaattgtag accttgcgca gttactaaat aaaatgggag ctaaagtcaa gggtgctggt
     7501 acggaaacat taacgatcat tggtgttgat gctttacatg gtacagaaca tgatgttgtc
     7561 caagatcgta ttgaagctgg aacctttatg gtggcagctg ctatgacttc tggaaatgtt
     7621 ctagtaaagg atgctatttg ggaacataac cgtcctttaa tctctaaatt gatggagatg
     7681 ggtgttgaag tgtcagagga agaagatggt attcgtgtaa aagctgatac taaaaaattg
     7741 aagccagtga ctgtgaaaac actccctcat ccaggatttc ctactgatat gcaagctcaa
     7801 tttacagctc taatggcagt agttaatggc gaatcaacta tgattgagac agtttttgaa
     7861 aatcgttttc aacatttgga agaaatgcgt cgcatgggat tacaaacgga aattttacga
     7921 gatactgcaa tgattcatgg tgggagagcc ttacaaggtg caccggtcat gtctactgac
     7981 ttacgagcta gtgcagcttt aatcttggca ggaatggttg cgcaagggca aacagtagtt
     8041 ggccaattaa cccatttaga ccgtggttat tatcaattcc atgaaaaatt agcggctctt
     8101 ggtgctaata ttaaacgtgt aagtgaggcg taatatggca tctagtggtt ggaaatatgt
     8161 actaaaacaa atcggtctaa tagtattagt catactgtta gccttgctgt ttttagcggt
     8221 tggattgatg ttgggttata gtgtttttgg tgacggagag catgcttact ctattttatc
     8281 gctggataaa tggcaaaata ttattggaaa atttttagga aaatagaaag agccactata
     8341 agttatttga tgcttatagt ggtttccttt gagagttaat tttagcagta gaataataca
     8401 atgacaaaaa aacaaaaaca aataacagct tcgattgtaa ctttgctttt ttcattactg
     8461 gcaatagggt taggtgaaag taaatctttg cctaatgatc acattttaaa acaggtgaca
     8521 gatttaatta ttagtaatga atctggcaaa aatacttccc ttaaaaatat atcaggaacc
     8581 cctagtagag agttatctga atctgttttg actagcaatg ttaaaaaaca attgggaaca
     8641 aatatagctt ggaaccagtc aggtgctttt atcattaatc aaaataaaac agatttgaat
     8701 gctaaagtgt caagtgcacc atatgctatt aatgaaataa agaaggttaa taaccaaatt
     8761 gttccaacta aagcaaatgc cttattaaca aaagcaactc gtcaatatcg aaatcgagaa
     8821 gaaacaggga atggtagaac ttattggaaa ccagctggat ggcatcagat aaatggatta
     8881 aaagggagct ataaccacgc tgtggacaga ggccatttaa ttggttatgc tttagtaggg
     8941 agtttaagag gatttgatgc ctccacaagt aatcctaaaa atattgccac acaagcagct
     9001 tgggcaaacc aagctaatag caaccaatca acagggcaaa attattatga aactttagtt
     9061 cgtaaagcat tggatcgtca taaaactgtt aggtatcgtg tgactttaat ttatgacaga
     9121 gataatcttt tatcttcggg atcacatatt gaggctaaat cttcagatgg aagtttagaa
     9181 tttaatgttt ttattcctaa tgtacaatcg ggattattat ttgattatgc aacaggaaaa
     9241 gtgaagcaga caaagtaatc tttatctaat agtgcttgtt aaagactgaa aaatttgata
     9301 aaatagtcct taattgaata gaggtctgtg agactagtaa accaagggaa gctgcttagg
     9361 gagttcagcc gtgactgcaa gctgaatcag tgaaagaggg tggaattcac tcacaaatag
     9421 gactataatt aaggtctgat tggtcgatca gatagaaaac ggatggtacc gcgtgtcaac
     9481 gctccgatta agggggagtg gcacgctttt tttgttttca aagctactta ttttgacttg
     9541 gaggaaagat ggatttacaa aaacaattag aagagttgaa aatatcaact caagaaaagc
     9601 ttaaagaaat gactgggaac cacactaaag aattacaaga cttacgtgta caagttttag
     9661 gaaaaaaagg ttctctaaca gaattactaa aaggtctgaa agatttatct aatgatttac
     9721 gaccagttgt tggaaaacaa gtcaatgaag tacgtgatat tttaacaaaa gcgtttgagg
     9781 aacaagctaa agtagtggaa gctgcgaaga ttcaagcaca gttagagtct gagagtgtcg
     9841 acgttacctt gcctggtcgt caaatgacat taggtcaccg tcatgtctta acacaaacta
     9901 gcgaagagat tgaagatatc tttttaggta tgggattcca agtggttgat ggttttgaag
     9961 ttgagaaaga ctactataat tttgaacgaa tgaatttacc aaaagatcat ccagcccgtg
    10021 atatgcaaga tactttttat attacagaag aaatcttgct acgtactcat acttcgcctg
    10081 ttcaagcacg aacaatggac caacatgatt tttctaaagg gcctttaaaa atgatttctc
    10141 ctggacgtgt tttccgtcgt gatactgatg atgctactca tagccatcaa ttccatcaaa
    10201 ttgaaggctt agttgttgga gaaaatattt caatgggcga tctcaaagga acacttcaac
    10261 tgatttctca aaaaatgttt ggagcagagc gtaaaatccg acttcgtcct tcatatttcc
    10321 cattcacaga accttcagtt gaagttgatg tctcttgctt caagtgcgga ggtaagggat
    10381 gtaatgtctg caaacagaca ggttggattg agatattggg tgctggtatg gttcacccaa
    10441 gtgttttgga aatgtctggt attgattctg agaaatactc cggttttgcc tttggcttag
    10501 gtcaagaacg tattgcaatg ttacgctacg gtatcaatga cattcgtggc ttctatcaag
    10561 gtgatgttcg cttcacagat caattttaag attttagata gcaagtagca tcattggtgt
    10621 gatgataaat ttatgttaac gctattacct tactcaatag gaggttttct aatgtctcaa
    10681 attactttta aaaaggttgg ccttgataat gtgaatattt tacagaatct tgctatagaa
    10741 acctttcggc agacgttttc tcatgataat agtgaggaac aacttcaagc atttttcaat
    10801 gagtcttaca ctttaccagt attaaaatcg gaaattaccc atgctgaatc tgatacttat
    10861 tttgtttatc ttgatacaga tttagtgggt tatttaaaag taaactgggg aagtcagcaa
    10921 actgaaaaaa acttgataaa gcatttgaaa ttcaaaggat ttatcttcta gatgcctacc
    10981 aagggaaagg tataggtaag gctactttcg aatttgcttt ggatttagct tataaatcag
    11041 gcttagattg ggcttggtta ggtgtttggg agtttaatca taaagctcag gcattttacg
    11101 ctaaatacgg atttgaaaaa ttttctgagc accaattttc agtaggtgat aaagtggata
    11161 cggattggtt attaagaaaa agtttacact aaaaactaca aagcacttgt ataaaaataa
    11221 aagaaaataa atagagagaa attacatgtt agtatcgtat aaatggttaa aagagttagt
    11281 ggatgttgat gtcacaacag cagaactagc tgagaaaatg tcaaccactg gtattgaggt
    11341 tgaaggtgtg gagacaccag cggaaggttt atcaaaatta gttgtaggtc atattgtttc
    11401 gtgtgaagat gttccagata cccacttaca tttgtgtcaa gttgatacag gggacgatga
    11461 acttcgtcaa gttgtctgtg gtgctccaaa tgtcaagact ggtattaatg ttatcgtagc
    11521 ggttcctggt gctcgtattg cagataacta taagattaaa aaaggtaaaa tccgtggtat
    11581 ggagtcactt ggaatgattt gttcacttca agagttgggc ttatcggaaa gtatcattcc
    11641 aaaagagttt tctgatggta tccaaatttt acctgaggga gctatacctg gtgatagtat
    11701 tttctcttat cttgacttag atgatgaaat tattgaatta tcaattacac caaaccgtgc
    11761 agatgctctt tcaatgcgtg gtgttgccca tgaggtagca gcaatttatg ggaaaaaagt
    11821 tcattttgaa gaaaaaaatc tcattgaaga agcagaaaga gcagctgata agatttctgt
    11881 tgtcattgaa tcagataaag ttttatctta ctcggctcgt attgttaaga atgtaacagt
    11941 tgcaccaagt ccacaatggc ttcaaaataa actgatgaat gctggtatac gtccaattaa
    12001 taatgttgtt gatgtgacaa actatgttct tttgacttat ggtcaaccaa tgcatgcttt
    12061 tgattttgat aaatttgatg ggacaactat agtagcacgt aatgctgaga atggtgaaaa
    12121 gttaattact cttgatggtg aagagcgtga cttaatagct gatgacttgg tcattgcggt
    12181 aaatgatcaa ccagttgctt tggcaggcgt tatgggagga caatcaactg aaattgatag
    12241 ttcttctaag acagtggtcc ttgaagcggc agtatttaat ggaacaagta tccgcaaaac
    12301 aagtggaaga ctcaaccttc gttcagagtc atcttctcgc tttgaaaaag gtatcaatta
    12361 tgatacggtt tcagaggcta tggattttgc ggctgcaatg ttacaagaat tggcaggcag
    12421 tcaagttctt tctgggcaag ttacagaagg agttcttcca actgagccgg tagaggtatc
    12481 aacgactctt ggttacgtta atactcgttt gggaacagaa cttacctata ctgatattga
    12541 agaagtattt gaaaaactcg gctttgctat ttctggttca gaagtgaaat ttactgtgtt
    12601 ggtaccgcgt cgtagatggg atattgccat tcaagctgac ttagttgaag agattgcccg
    12661 tatttatggt tacgaaaaat taccaacaac attgccagaa gccggagcga ctgcaggaga
    12721 attaacgagt atgcaacgct tacgtcgtag agtccgtacg gttgctgaag gagtaggatt
    12781 atctgaaatt atcacctatg ctttaacgac tccagaaaaa gcagtgcagt tctcaactca
    12841 agcaactaat ataactgaac ttatgtggcc gatgactgtt gatcgctcag cacttcgcca
    12901 aaatgttgtt tcaggaatgc ttgatacaat tgcttataac gttgctcgca aaaacagtaa
    12961 ccttgcagtt tatgagatag gaaaagtttt tgaacaaact ggtaatccaa aagaagattt
    13021 accaactgaa gtagagacgt ttacttttgc cttaacaggt ctagtagagg aaaaagattt
    13081 ccaaacaaaa gccaagccag ttgatttctt ctacgccaaa ggtattgtag aggcgctctt
    13141 tattaaatta aaattagatg ttacttttgt agcacaaaaa ggtttagcaa gtatgcaccc
    13201 aggtcgtaca gcaactatct tattagatgg gaaagagatt ggttttgttg gtcaagtaca
    13261 cccacaaact gcaaaacaat atgatattcc tgaaacttat gttgctgaaa tcaatctatc
    13321 aactattgaa agtcaaatga accaagcgtt gatttttgaa gatattacga agtatccgtc
    13381 tgtatctcgc gatattgcat tattacttgc agaatctgtt agtcatcatg atattgtgtt
    13441 agcaattgag tcatcagggg taaaacgtct aacagcgatt aaactgtttg atgtctacgc
    13501 tggaaataat attgcagaag ggtataaatc aatggcttat agcttaactt tccaaaaccc
    13561 taatgacaat ctaacggacg aagaagtggc taaatatatg gaaaaaatca ctaaatcact
    13621 cgttgagaaa gttaatgcag aaataagata atttatatct tacgttgaaa aataaataaa
    13681 aaacagcatc aattaagatg ctgttttttt atttaaaatt tatagatgtt ctgcggagaa
    13741 agtatcaccg tgttggatgt caccatattg aaagcctttg ttaaaccagc gttggcgttg
    13801 ttgagctgtt ccatgggtaa agctatcagg cactaatttt ccgtaggttt ctttctgaag
    13861 ggtatcgtct ccgacggcgt gggcagcatt catggcctct tcaaagtctc cttgttctaa
    13921 gagatttttt cccctgatgt agtgagccca tacccctgca taataatctg cttgaagttc
    13981 tagccgaaca tttaaagcat ttgcttcttt cttagtaagt ccgtgtcgca ttctattata
    14041 cttatccata atgcctaact ctgtttgaat gtggtgacca acttcatggg cgatgacgta
    14101 ggccatagca aaatcaccag tagcaccata tttatgtgat aattcattgt aaaaagaaat
    14161 atcaagatag atttttttat ctgctgaaca ataaaatggt tctgaagcag attcacctat
    14221 accacaacct gtttgaatcg aattggtgta aagaacaagt tttggttcct tataatttcc
    14281 aaaaccttgg gtttggaatt cttgtgacca gaaatcctca gttgatccaa ggactttatt
    14341 aacgaaatcg atttgttctc tcgttgcgct attatcaaca gaacgtgaga cattctgaga
    14401 ttggtaacta gaaggtgagg atgagtcatt aaaaatgctg gttagtcccc ctccgccaag
    14461 tagcagtaag atgattaaaa ccacaagctt ggttttccaa ctccctcgca tcaataaaag
    14521 ttgaagaatc ggtaatccac ttcctccgct agagaatgaa cctccgctac tggagcgacg
    14581 atcttcaaca ttgtcgcttt ttcttaggtc atctattttc ataagtatct ccaaagtgtt
    14641 ttataggctc attataaact attttaataa gaaatgcttg aatttttaac taaatatctt
    14701 cccctagatt tgaagctatt tttggtataa tattaataaa ctgtaaagaa taagttgaag
    14761 aaggtttatt tctcagttga cttataagat aggagattca tgaaattact ttatactgat
    14821 attaatcatg atatgacaga aatcttagtc aatcaggccg cgcatgctgc tgaagctgga
    14881 tggcgtattt tttatattgc tccaaactct ttatcttttg aaaaggagcg tgcagtatta
    14941 gagacccttc ctcaagaagc ctcttttgct attacaatta ctcgctttgc tcaattggca
    15001 cgttatttta ctttaaacca gcctaatcaa aaagaaagtt taaacgatat tggattagca
    15061 atgatctttt atcgtgcttt agcaagtttt gaagatggac aattaaaagt ttttggacga
    15121 ttgaagcaag atgcttcctt tatcagtcaa ttggtagacc tatacaaaga attgcagaca
    15181 gcaaatctga gtattttaga tttaaaatat ttacattctc ctgaaaaatt tgaagatcta
    15241 ttagctattt ttctagtggt ttcagatctt ttaagagaag gagaatacga caatcaatct
    15301 aaaattgcct tttttacaga acaagtaagg tcaggacagc tagatgtgga tttaaagaac
    15361 actatcctta tagtcgatgg ttttacccgt ttctcagctg aagaagaagc tttaattaaa
    15421 agtctgagtt cacgttgtca agagattatc atcggagctt atgctagcca aaaagcgtat
    15481 aagactaact ttaccaatgg aaatatttat tctgcagggg ttgatttttt aagatatcta
    15541 gcaacgacat ttcaaacaaa accagaattt atcttgtcta agtgggaaag taagtctggt
    15601 tttgaaatga tttcgaaaaa tatcgaaggt aaacatgatt ttacaaatag tagtcacatt
    15661 ttagacgaca ctgctaaaga ttgtataaca atttgggagt gcattaatca aaaagatgaa
    15721 gtggaacatg tggctagagc tatacgtcag aaactttatc agggatatcg ctacaaagat
    15781 attttagttt tactaggaga tgtagatagt tataaactac aattaagtaa gatttttgaa
    15841 cagtatgata ttccttatta tttcggtaaa gctgaaacaa tggctgcgca ccctttagtt
    15901 cattttatgg attccttgtc gcgaataaaa cgttatcgat ttagagctga agatgtttta
    15961 aatcttttta agacaggtat ttatggagag attggtcaag atgatttaga ttattttgaa
    16021 gcttatatct catatgcaga tattaaaggt cctaagaagt tctttacaga ttttgttgtg
    16081 ggggctaaaa aatttgattt agggcgtctt aacactattc gtcagtccct ccttactcca
    16141 ctggaaagtt ttgttaaaac gaaaaaacaa gacggtataa aaacactcaa ccaattcatg
    16201 tttttcctta ctcaggtagg tttgagtgac aatttatcgc gattagttgg tcaaatgtct
    16261 gaaaatgaac aagaaaaaca tcaagaagtt tggaaaacat ttacagatat tttagagcag
    16321 tttcaaacaa tatttggtca agaaaaatta aatttagacg aatttttatc gttattaaat
    16381 tcaggtatga tgcaagccga gtatcgtatg gtccctgcta ctgttgatgt ggttactgtt
    16441 aagtcttacg acttggtaga accacatagt aatcaatttg tctatgccct aggcatgaca
    16501 caatcacatt ttccaaagat tgctcaaaac aaatctttaa tatctgatat tgaacgtcag
    16561 cttataaatg atgccaatga cactgatggt cattttgaca ttatgacgcg agaaaatttg
    16621 aagaaaaacc attttgctgc tctctctctt tttaatgctg ctaaacaagc attagtgttg
    16681 actataccac aattattgaa tgagtctgaa gatcagatgt caccatatct tgtcgaacta
    16741 agagatatag gtgttccttt taatcataaa ggacgccaat cgttaaaaga agaagctgat
    16801 aatattggga attacaaagc tttattatcg cgtgttgttg acttgtatcg ctctgctatt
    16861 gacaaagaga tgactaaaga agaacaaact ttttggtcag ttgctgttcg ttatttgcgt
    16921 cgtcaattaa cttctaaagg gatcgagata ccaataatta cagatagtct tgatactgtg
    16981 acagtttcat cagatgtgat gaccagacgc ttcccagaag atcacccact taaattatcc
    17041 tcatctgctt tgacaacttt ttataataat caatataagt attttttaca atatgtgtta
    17101 ggtttggaag aacaagactc aattcatcca gatatgcggc accacggaac gtatcttcat
    17161 cgtgtttttg aaattctgat gaaaaaccaa gggatagaat cttttgaaga gaaactcaac
    17221 tctgcaatta ataaaaccaa tcaagaagat gttttcaaat ccttgtattc agaagatgca
    17281 gaaagtcgtt attctttaga aattttagaa gatatcgcgc gtgcaactgc aaccatatta
    17341 cgacaagata gtcagatgac tgttgaaagt gaagaagaac gatttgaact aatgattgat
    17401 aatactataa aaattaacgg tattattgac cgtatcgatc gtttatctga tggttctttg
    17461 ggtgtcgtgg actataaatc aagcgctcaa aaattcgata tccaaaaatt ttataacggt
    17521 ctaagtccac aattagtcac ctacattgat gctatcagtc gtgacaagga agttgaacaa
    17581 aaaccaccga tttttggagc tatgtactta catatgcaag aacctagaca agacttgtct
    17641 aaaattaaaa atttagatga tttagtaaca aaaaatcatc aagctttaac ttataaagga
    17701 ttattttcag aagccgaaaa agaattctta gcaaatggga aataccatct caaagattcc
    17761 ctttattcag aggctgaaat tgctatttta caagcacata atcaatcgct ttacaaaaaa
    17821 gcatctgaga ctattaagtc aggtaagttt ttgatcaatc catatacaga agatgctaaa
    17881 acagttgacg gtgatcagtt taagagtatt acggggttcg aggcggatag gcatatggca
    17941 cgtgccagag cgctttataa attacctgct aaggaaaaac gtcaaggttt cttaacatta
    18001 atgcagcaag aggaggaaaa tgatgacctt taagcctttc ttaaatccag aagatattgc
    18061 tgttattcag acagaagaaa aaaattctga taaaaaacaa aagcgaacgc ctgaacaaat
    18121 tgaagcaatt tatacttttg gtaacaatgt cctggtatca gcttcggctg gttctggaaa
    18181 gacttttgta atggttgagc ggattttaga taaactttta agaggggttc cgattgagtc
    18241 tttgtttatc tcaacattta cagtaaaagc ggcaggtgag ctcaaagaac gtttagaaaa
    18301 gaaaataaac gagtctttga aatccgcaga atcagatgat ttaaaacagt ttctcaccca
    18361 gcaacttgtc ggcattcaaa cagctgatat tggtactatg gatgctttta cgcaaaaaat
    18421 agttaaccaa tatggttata ctttgggcat atcacctata tttaggattt tacaagataa
    18481 aaatgagcaa gacgtcataa agaatgaggt ctatgctgat ttattttctg actacatgac
    18541 tgggaagaat gcagcttcat tcataaaatt agtcaaaaat ttctcaggta atcgtaaaga
    18601 tagcaaagct tttcgagaaa tggtttataa agtttatgct tttagccagt ccatcgataa
    18661 ccccaagcgc tggatgcaaa cagtttttct aaaaggtgca caaacttaca ctgattttga
    18721 agctattcca gatcaagaag tttcaagcct tttaaatgta atgcaaacaa ctgctaatca
    18781 attacgtgat ttaacggatc aagaagatta taagcaatta actgctaaag gggttccgac
    18841 agcaaattac aaaaaacacc ttaaaataat tgaaaattta gtccattggt ctcaagactt
    18901 taatttgctg tatggaaaaa aaggattggc taatttggca cgtgatatca ctaatgtgat
    18961 tccttcaggc aatgatgtta cagttgctgg cgttaaatat cctatcttta aacaattgca
    19021 taatcgtatc gttggactta agcacttaga agttattttt aagtatcaag gagaatctct
    19081 tttcttgtta gaattattgc aatcctttgt tttagatttc tcagaacaat atttacagga
    19141 gaagattcaa gaaaatgctt ttgaattttc tgatattgcc cattttgcaa ttcaaatatt
    19201 ggaagaaaat catgatatta ggcaaattta tcaagataag tatcatgaag taatggttga
    19261 tgagtatcag gacaataacc atacccaaga gagaatatta gaattattgt ctaatggaca
    19321 taatcgtttc atggttggag acataaaaca atcaatttac cgttttcgtc aggctgatcc
    19381 acaaattttt aatgataaat acaaagctta ccaagacaat ccaagtcaag ggaaattaat
    19441 tattctgaaa gagaattttc ggagtcaatc agaggtttta gacagtacaa atagtgtgtt
    19501 tacacatctt atggatgaag aagtaggaga cattctttat gatgaaagtc accaattaaa
    19561 agctggtagt ccaagacagc aagaaagaca tccgaataat aaaacacaag ttttactatt
    19621 agatactgat gaagatgata tagatgacag tgatagtcaa caatatgata ttagcccagc
    19681 tgaagcaaag ttagtcgcaa aagaaattat tcgattgcat aaagaagaga atgttccctt
    19741 tcaagatatc accctcttag tttcttcaag gacaaggaat gatggtatct tacaaacttt
    19801 tgaccgttat ggtattcctt tggtaactga cggtggggag caaaattact taaaatcagt
    19861 cgaagtgatg gtaatgcttg atactttgcg atcaattgat aatcccttaa atgattatgc
    19921 tctagttgct cttttgagat ctcccatgtt tggctttaat gaagatgatt taacaagaat
    19981 tgctattcat gatgtcaaaa tggcttttta tcataaagtg aaattgtctt atcataaaga
    20041 aggtcatcat agtgatttaa ttactccaga attatcttct aaaatagatt actttatgaa
    20101 gacattccag acttggcgtg attttgctaa atggcattcg ctttatgatt taatatggaa
    20161 aatatataat gaccgttttt attatgatta tgtaggagct ctgcctaaag ctgagcaacg
    20221 tcaagccaat ctatatgcct tggcacttcg agcaaatcag tttgaaaaaa caggtttcaa
    20281 gggtttatca cgttttattc gaatgattga taaagtttta gaaaatgaaa atgatttggc
    20341 agatgttgag gtggcattac cgcaaaatgc tgttaaccta atgactatac acaaatcaaa
    20401 gggtcttgag tttaagtatg tatttatttt aaatattgat aagaaatttt caatggtaga
    20461 cataacttcc cctcttattt taagccgaaa tcaaggaatt ggtattaaat atgttgctga
    20521 tatgcgacat gaactcgaag aagagatact gcctgcagta aaggttagca tggaaacact
    20581 tccttaccaa cttaataaaa gagagttacg cttagctact ttatcagagc aaatgcgttt
    20641 actttacgta gctatgacac gtgcagagaa aaaattatat ctggttggta aggctagtca
    20701 aaccaaatgg gctgatcatt atgacttagt atctgaaaat aattatctcc cattagctag
    20761 tcgtgagact tttgtgacct ttcaagattg gttactggct gttcatgaaa cctataagaa
    20821 acaagagttg ttttatgaca tcaacttcgt atcattagag gaattaacag atcaccacat
    20881 agggatggtt aatcctagtc tacctttcaa tcccgataat aaggcagaga atcgtcagtc
    20941 tgaagatatc gtgcacgcga ttagcgtgtt agaatctgtt gaacagatta atcaaactta
    21001 taaagcggct attgagttac cgacagtaag aacaccaagt caagtcaaaa aattctatga
    21061 gcctattcta gatatagaag gcgttgatgt gatggaaact attactaaaa catcagtaga
    21121 ttttaagtta cctgattttt ctacaagtaa aaagcaagac ccagcagctt taggatctgc
    21181 tgtacatgaa ttaatgcaac gcatagagat gtcatctcat gtcaaaatgg aagatattca
    21241 aaaagccttg acagaagtga atgctgaaac aagtatcaaa gcggctattc agattgaaaa
    21301 aatcaactat tttttccaag aaaactcact cggtaaatat atacaagaag aagtagaaca
    21361 tctacatcga gaggcacctt ttgccatgtt aaaagaagat ccggagagtg gtgagaaatt
    21421 cgttgtacgt ggtattattg atgggtatct actattagaa aatcggataa ttctatttga
    21481 ttataagact gataaatttg tcaatcctct ggaattaaaa gaacgctatc aggggcaaat
    21541 ggctttatat gcagaagctt taaaaaaatc ttacgagata gaaaggatag acaaatactt
    21601 aattctttta ggtggtaaac agctagaagt ggtaaaaatg gattaaagaa aggaaaaaat
    21661 gatagtcgaa caaaaatttg gaaatggttt tacttggatt aacattgaag cagagcaatt
    21721 gcgaacagaa acatcggaaa ttcaagcaaa atatttggat agtgagatta ttacatatgc
    21781 tctggatgat tatgaacgag cctttatgga atgttctcat attaaaggaa aagaagtctt
    21841 gacgattatt tttaatacta ttgatctaaa gcagaaagaa agttattatg agaccgttcc
    21901 gatgaccttt tgcttatcac acgaccgttt gataacggtg acacggtctc gaaatagtta
    21961 tatgctagaa ctattgcaaa aatatctaga ccgtaatcca gatgtctctc ctaaaaaatt
    22021 tttatttgca gctttaactt tgattacaaa acaatatttt aatgttgtat cgaaaattga
    22081 tagagaaaag gatattctca accgtcaatt gagagaacaa acaaccaata agcgcttatt
    22141 agccatgagt gacctagaga caggctccgt ttatcttttg acagctgcca atcaaaatgc
    22201 attggtatta gagcaattag atgttcatcc tagtcagcga tttaattctg aagtggaaaa
    22261 agaacagtta tcagatgctc tcattgaagc gcatcagtta gtcagtatga cacaattaaa
    22321 cagtcaggta ctttcacaat tatcatctac ttttaataat gtcttaaata ataacttgaa
    22381 cgaaaactta acaggtttga atatcatttc gattaattta gcgattattg cagcaattac
    22441 tggctttttt gggatgaata ttccattacc tcttacagaa tcacggagtt catggttaat
    22501 tgttattgct acttcggtct tactttgggt tattattgct caaattttaa aacgagtttt
    22561 taacttaaga aaataaaaat gttcagtgcg taagcaccga acatttttta tttacctaag
    22621 cagaattggc tgaagagctg agttattagc tcatctggag ctgcatcccc agtgatttct
    22681 ccaagtattt cccaagttct agtcatatcg acttgtaaga gatcaacagg cattcctaat
    22741 tccaagccct cattgacagc ttttaaacta tcaacggctt tttcaattag tgagatatgg
    22801 cgagcattcg atagataagt agcgtcatgt tctaccatac cagcattgtc aaagaaaata
    22861 tcgttaatac gttcttcgat tttatcgatg ttttcatttt ctaaaaccga aattggaata
    22921 acattttcag gtaattcgtt aacttctatt ttttgaggaa gatccgtttt attgagtaat
    22981 acgatacgat tactttcttt acttagttct agaagggagc ggtcttgtaa tgttagtggt
    23041 tcggaactat tgagaaccaa caacacaagg tcggcttctt ctaaagcctt ctttgaacgt
    23101 tcaacaccaa ttttttcaac gatatcatct gtatcacgga taccagcagt gtcaactagt
    23161 tttaaaggaa ctcctttaat attgacgtat tcttcaataa catcacgagt tgttccttca
    23221 atatctgtca caatcgcctt ttcttcacgt aagaggttat ttagtaagct ggatttaccg
    23281 acatttggac gaccaatgat tgctgttgaa agtccttctc gaagaatttt tccatgtcgg
    23341 gcagttcgaa gtaaattttc cataagtgct tgaaactctt gtgttttttc acgcatcagt
    23401 gttgtagtca tctcttcaac gtcatcatat tcagggtaat cgatattaac ttcaacttgg
    23461 gctagagtat ttagaatttc ttgtcgagtg ttattgatta gtgtctttaa agaaccatct
    23521 aattgcttaa cggcaatatc cattgcttta tctgttttag ctctaataag atccataaca
    23581 gcttcagcct gagtcaagtc aactcgaccg tttaaaaaag cacgtttagt aaattctcct
    23641 ggttcagcca ttcgtgctcc atgtcgtaaa attaactgaa gaatttcgtt agtaactgca
    23701 attcctccat gcgtattaat ttcaataaca tcttctcttg tgaatgtttt tggagcaagc
    23761 ataacgccta acataacttc atccaagatt tcatttttat ctgggtccac aatatgacca
    23821 tagtttaagg tatgggattg gatagcggat agatcttttc cacgataaat ttttgatgct
    23881 atctttaagg catctgtacc agatatacgg acaataccaa tagctccctc accgagagga
    23941 gttgaaatag ctgcgatggt atcaaattct tttgtaatag acataaagtc tctctctttc
    24001 ttgggaatta ctaacattca ttgtaaccta aagccctaaa aggtgcaatc atttgattaa
    24061 aagagaagat ttccataatg gttttatttg aaggtatgct ataataaaac tatgattata
    24121 ctacaaggaa acaaaattga gcgctcattt tcaggtgatg tgctttttga taatattaat
    24181 attcaagttg atcaacgtga tcgtattgct cttgttggac gtaatggtgc tggcaaatca
    24241 acgcttctta aaattttagt tggtgaagag gctccgacaa aaggagaaat aaataaaaag
    24301 cgtgatttga gtttgtccta cttggcacaa gatagtcgtt ttcaatctga aaatacaatt
    24361 ttccaagaaa tgttacaagt ttttgattca ttaagagaag tagagaagcg cttacgtgaa
    24421 ttggaattac aaatgggaca agtttcagga agtgacctgg aacaattgat gaaaacatac
    24481 gatattttgt cagaagagtt tcgtgaaaaa ggtggcttta cctatgaatc agatataaag
    24541 gctattttaa atggctttaa gtttaacagt gatatgtggg aaatgcctat tagtgaattg
    24601 tctggtgggc aaaatacacg attagcgctt gcaaaaatgc tacttgaaaa gccagaatta
    24661 cttgttttag atgaaccaac gaaccattta gacattgata cgattgcttg gttagaaaat
    24721 tatcttgtaa attatcaagg agcccttatt attgtcagcc atgaccgcta ttttttagac
    24781 aaggtagcta cagtaactta tgatttaacg acacattctc tagaccgcta tgtaggcaat
    24841 tattcgaaat ttatggatct taaggcagag aaaatagcga ctgaagaaaa gaattttgaa
    24901 aagcaacaaa aagaaattgc taagttagaa gattttgtac aacgtaacat tgttagggct
    24961 tcaaccacta aacgagcaca agctcgtcgt aaacaattag aaaagatgga acgactcgac
    25021 aaaccgaatg tagagcaaaa atcagctaat atgacttttc atgctgggaa agtctcaggg
    25081 aatgttgttt taacacttga gaatgcagct attggttatg aaggtgtttc tttatcggag
    25141 ccaatcgatt tggacgtgaa gaaatttgat gctattgcaa ttgtaggtcc aaatggtatc
    25201 gggaaatcaa ctttaatcaa atcgcttgtt gggcaaattc cttttatcaa aggagaagca
    25261 aaattaggag caaatgtcga aactgggtat tacgatcaaa gtcaaagtaa tttaacaaaa
    25321 acgaatactg ttttagatga gttatggaat gctttttcaa caactccaga agttgaaatt
    25381 agaaaccgtt taggagcttt tcttttttca ggtgatgatg ttaaaaaatc agtttcaatg
    25441 ctatccggtg gtgaacgagc gcgtttgcta ttggcaaaat tatctatgga aaataataat
    25501 ttcctcatat tagatgagcc aacaaaccac ttggatattg atagtaaaga agtcttagaa
    25561 aatgcactca tcgaatttga tggcacatta ctttttgtta gtcatgatcg ctattttatt
    25621 aatcgtgtgg caactaaggt tctcgaaatt tctgataagg gaagtactct ctatctagga
    25681 gattatgatt attacctcac taagaaagcc gaactagagg aattagcccg tttaaatgaa
    25741 gaagaagtga gtgcttcaaa aacagaaatc gacgttacat cagattatga gacacaaaaa
    25801 gcaaatcaaa aggaatttag gaaaattacc cgtcgcgttg cagaaattga ggcacgtttg
    25861 gaagttcttg aaaatgatga aaacaatatc aatggtttaa tgttagaaac caatgacatc
    25921 ggaaaactct ctgatttaca aaaagagctg gaatcaatcc aagaagaaca attgctatta
    25981 atggaagaat gggaaaatct aaatatgaga ctagatttgt gagaaaattt caaaaacaat
    26041 agtttgtgaa aaaaagtaca ttttaaattg agatgccttt tacgaaaaca aagaaagcgg
    26101 ttcaaattct tgcatcttgt gaaaaaaact tatacaatga ttaagtaata aagatttttt
    26161 tggaggttag aatggtaact ctatctaaag aacaacactt ggatatgttt ctaaaaatgc
    26221 aacgtattcg tgatgttgat atgaaattta acaaattggt tcgtcgtggt ttcgtccaag
    26281 ggatgactca cttctctgtt ggtgaagaag ctgcttcagt aggtgctatc caagatttaa
    26341 cagattcaga tatcattttc tcaaaccacc gtggacacgg acaaactatt gcaaaaggta
    26401 ttgatattgg tggaatgttt gctgaacttg ctggtaaagc aacaggaaca tcaaaaggtc
    26461 gtggaggctc aatgcactta gcaaaccttg aaaaaggtaa ctatggaaca aacggtatcg
    26521 tcggtggagg atatgcgctt gcggtaggtg ctgcccttac acagcaatat gaaggaactg
    26581 ataacattgt tatcgccttt tcgggtgact cagcgactaa cgaaggctca ttccatgagt
    26641 cagttaactt agctgctgtt tggaacttac cagttatctt ctttatcatc aacaaccgtt
    26701 atggtatctc aacagacatc acatactcaa ctaaaattcc tcacctttac atgcgtgctg
    26761 atgcgtacgg tattccaggg cattatgttg aagatggtaa tgatcttatg gctgtttacg
    26821 agaaaatgca tgaagttatc aattacgtgc gttcaggaaa tggaccagct attgtagagg
    26881 ttgaatcata ccgttggttt ggtcattcaa ctgctgatgc tggggtatac cgtactaaag
    26941 aagaagttga ttcatggaaa gctaaagatc cagtaaaacg ctaccgtgca tacttaattg
    27001 aaaatgaaat cgctactgaa gaagagcttg cagctattga agcacaagtg attaaagaag
    27061 ttgaagaagg ggttaaattc gctgaagaaa gtccattccc tgatatgtct gttgcatttg
    27121 aagacgtttt tgtggactaa aataacctaa agtagatgag atttatctcg ttctattatt
    27181 caattgatta aaaaacggat attcggagga aataatgagc gaaactaaag ttatggcttt
    27241 gcgcgaagcg attaatgtcg ctatgagcga agaaatgcgc aaagatgaaa aagtattttt
    27301 aatgggtgaa gatgtaggtg tatatggagg agacttcggt acatctgttg gtatgttaga
    27361 agagtttggt gctaagcgcg ttcgcgacac accaatctca gaggctgcga ttgcaggttc
    27421 agctatcggt gctgcacaaa caggtttgcg tcctatcgtt gatttgacat tcatggattt
    27481 cgtaacaatc gcaatggatg ctatcgttaa ccaaggtgct aaaacgaatt atatgtttgg
    27541 tggaggtcta tcaactccag ttactttccg tgtggcatca gggtcaggta ttggttcggc
    27601 tgcacaacac tcacaatcat tagaagcttg gttaacacac attccaggtc taaaagttgt
    27661 tgcacctggt actgttaatg agtcaaaagc acttttgaaa tcttctattc ttgacaataa
    27721 cccagttatc ttcttagagc caaaagctct ctatggtaag aaagaagaag ttaatatgga
    27781 tcctgatttc tatattccac ttggtaaagg ggatattaag cgtgaaggta ctgacttaac
    27841 aattgtttct tatggccgta tgcttgagcg tgttatgcaa gctgctgaag aagttgctga
    27901 ggaaggtatt aacgttgagg ttgttgatcc acgtacactt attccgcttg ataaagaatt
    27961 aattatcgat tctgttaaga aaactggtaa attgatcttg gttaacgatg cttataaaac
    28021 tggtggtttc actggtgaaa ttgcaactat ggttgctgaa agtgaagcat ttgattacct
    28081 tgatcatcct atcgtgcgtc ttgcaagtga agatgttcca gtaccgtatt cacgtgtact
    28141 tgaacaagga atcttacctg atgtagcaaa aattaaagat gctatctaca aagtagttaa
    28201 caaaggtaaa taattcataa aacaaagagg gactgaggaa tgagtgaaaa cttagcctct
    28261 ttccttgttt ataggtttta atttatatcg ttagatgaaa catatatttg acgatatagt
    28321 ttagtgtgaa aggaaaacta tggctgtcga aattattatg cctaaactcg gggttgacat
    28381 gcaagaaggc gaaatccttg agtggaaaaa acaggtaggt gatgttgtta atgaaggtga
    28441 cgtcctactt gaaattatgt cagataaaac aaatatggag attgaagcag aagactcagg
    28501 tgttttacta aaaatcacac atgggaatgg agatgttgtt cctgtaactg agacaattgg
    28561 ctgtatcggt gctgaaggtg aagaagtaac agaagcatct tcttctgaaa atacatcagt
    28621 tgaagaaaac gcgacacaag taacttcaga accagaaaaa gttgaagaaa catcagaacc
    28681 aagtgtgcca gcagctacaa gtggtgaaaa agtacgtgcc actcctgcag ctcgtaaatt
    28741 agcaagagag atgtctattg accttgcact agtatctgga acaggggcaa atggtcgcgt
    28801 tcacagagaa gatgttgaaa gcttcaaggg tgctcaacca cgtattacac cattggcacg
    28861 tcgaattgct gaagatcaag gagttgatat agcagaaatc actggcagtg gtattcgtgg
    28921 taaaattgtt aaaaatgatg ttttggctgc tatgtcaccg caagcagcag aggctcctgt
    28981 aaaaacgaaa gctacaccaa caacagaaga aaaacaatta ccagaaggtg ttgaagtcat
    29041 taagatgtct gcaatgcgta aggctatttc taaaggtatg actaactctt acctcactgc
    29101 tccaagcttt acacttaact atgatattga tatgactgaa atgatggctc ttcgcaagaa
    29161 attaattgat ccaatcatgg ctaaaacagg cttgaaagtc agctttactg atttgattgg
    29221 aatggccgtt gttaagacat tgatgaaacc agagcatcga tacctaaatg cttcattaat
    29281 taacgatgca caagaaattg agcttcataa ttttgttaat attggaatcg cagttggtct
    29341 tgatgatggt ttgattgttc ctgttgttca caatgctgat caaatgtcac tttctgactt
    29401 tgttatagca tctaaagatg ttattaagaa aactcaagaa ggtaaattaa aatcagctga
    29461 aatgtcaggt tcaacattct caattactaa cttaggtatg tttggtacta agacgtttaa
    29521 cccaattatc aatcaaccga actcagcaat ccttggagta ggtgctacta ttccgactcc
    29581 aacagtggtt gatggtgaaa ttgtagctcg accaatcatg gcaatgtgtt taacaattga
    29641 ccaccgtatt gttgatggaa tgaatggtgc taagtttatg gttgatctta agaacttaat
    29701 ggaaaaccca tttggtttac ttatttgatt ccgtaggtct taatttgata taataagtta
    29761 ttcgaaaaac gaaagaggaa ataaaaaatg gcttttgatg taattatgcc aaaacttggg
    29821 gttgacatgc aagaaggcga aatccttgag tggaaaaaaa atgaaggtga caccgttaat
    29881 gaaggtgatg tccttcttga aatcatgtct gataaaacca atatggaaat tgaagcagaa
    29941 gacactggtg tacttttaaa aattgtacat caggcaggag atgttgttcc tgtcactgaa
    30001 gtgattgcct atatcggaga agaaggtgaa gaagtaggta cgtcatcacc ttcggctgat
    30061 gcaactatca cagctgaaga tggtcaatca gtttctggac ctgctgctcc ttcacaagag
    30121 acagttgcag cagcaactcc taaagaagaa cttgcagctg atgagtacga tatcgtagtc
    30181 gtaggtggtg gccctgcagg ttattacgca gcaatccgcg gtgctcaact tggaggaaaa
    30241 attgccattg ttgagaaaac tgaatttggc ggtacgtgtt tgaatgttgg ttgtatccct
    30301 acaaaaacat atcttaaaaa tgctgaaatc ctagatggtt tgaaagtagc agctggacgt
    30361 ggtatcaacc ttgcttcaac taactatgca attgatatgg ataagactgt tgcctttaaa
    30421 aattctgtag ttaagacatt gactggtggt gtacgtggtc tcttgaaagc gaacaaagtt
    30481 gaaattttca atggacttgg acaagtaaac cctgataaat ctgttgttat tggcgataaa
    30541 gttatcaaag gtcgtaacgt cgtccttgcg actggttcta aggtatctcg tattaatatt
    30601 ccaggtattg aatcaccatt agtattaaca tcagatgata ttcttgattt acgtgaaatt
    30661 cctaagtcac ttgctgttat gggtggtggt gtcgtaggta ttgagcttgg tcttgtatgg
    30721 gcttcatatg gtgttgacgt aactgttatt gaaatggctg atcgcatcat tccagcaatg
    30781 gataaagaag tatcgctaga acttcaaaaa atccttgcta agaagggaat gaagattaaa
    30841 acatcagttg gtgtttctga aattgttgaa gccaataatc aattaacgct taaattaaac
    30901 aacggcgaag aagttgttgc tgataaagct cttctttcaa ttggacgtgt tccacaaatg
    30961 aatggtcttg aaaatcttga gcctgaactt gagatggaac gtggacgtat taaagttaat
    31021 gcttatcaag aaacttcaat tccaggtatc tatgcaccag gtgatgttaa tggaactcgt
    31081 atgttagctc atgctgcata ccgtatgggt gaagtagctg ctgaaaacgc actcggcggt
    31141 aataagcgta aagcacatct agatttcact ccagcagctg tttatactca tcctgaagta
    31201 gcaatggtgg ggatgacaga agagcaagca cgtgaacaat atggtgacat attagttggt
    31261 aaaaatagct ttacaggcaa tggtcgtgct atcgcttcaa atgaagcaca tggttttgta
    31321 aaagtaattg ctgaacctaa gtataaagag attcttggtg ttcacattat tggtccagct
    31381 gcagcggaat tgattaatga agcttctacg attatggaaa atgagttgac tgtctatgat
    31441 gtagcacaat ctatccatgg tcacccaaca ttctctgaag ttatgtacga agctttctta
    31501 gatgttctcg gtgaagctat tcacaaccct ccaaaacgca aataattttg tttaatatat
    31561 ttgagattga gcttttatag cttcaatctc ttttcacgta tgtattgtct gaatattata
    31621 aaatattagt taggaattta aaatgaaata tatcgttaac acgtctaatg atccggctta
    31681 caatgttgct ttagaagctt acgcctttca aaagttgaca gatatcgatg aaatctttat
    31741 tctttggatt aatgaaccgg ctatcattat tggacgccat caaaatacaa ttcaagaaat
    31801 taacaaagag ttcattgata aaaatggcat ccatgttgtc cgtcgtttgt caggaggcgg
    31861 agcagtttat catgacttga ataatttaaa ctacactatt atttctaata atacacaaga
    31921 aggtgctttt gatttccaaa ctttttcaaa accagttatt gatactcttg ctaaattagg
    31981 tgttaaagca gaatttactg gtcgtaatga tttagaaatc aatggacaaa aatttgcagg
    32041 aaatgctcaa gcttattaca aaggtcgtat gatgcatcat ggttgtcttt tatttgatgt
    32101 tgatatgtca gtcttagggc aagcactcaa agtatctaaa gacaaaattg aatctaaagg
    32161 gataaaatct gtacgcgcac gtgttacaaa tattgttgac catctttcag ataaaattac
    32221 agtacaagaa tttagcgatg ccattttagc tcaagtgaag gaagaatatc cagagatgga
    32281 tgaatatgtt ttgagtgatg ccgagttatc agaaatccaa gctatgcgcg ataatcaatt
    32341 tgctacatgg gattggacgt atggtaaagc acctgaatac accattgaac gtggtgtccg
    32401 ctatccagct ggaaaaatta cgacttatgc taatgttgaa aattcaacga taaaatctgt
    32461 caaaatcttt ggtgacttct ttggagtgaa acctgtagat gatattgaaa aaatgttgga
    32521 aggtgtccgc tacgattaca aagatgtttt agcagcgctt aaaacagttg atacaagtca
    32581 atacttctca cgtatgacac ccgaagagat tacaaaagct attgtagact aaaatcaaaa
    32641 aacctagtca aagtctttgc ctttggctag gttttttgta tggttaagtg gccttatagt
    32701 acgacgggca tgtcgtacat ctgaggtgca agtcctctgt gggcacccgc taccggtgaa
    32761 cccaatagcg actcccaagc ttgactatcg tgaggtagcg gggagaggaa gggatagcga
    32821 aatcgtggct cta
//
LOCUS       DATRNX010000006        31929 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRNX010000006 DATRNX010000000
VERSION     DATRNX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31929
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            68..1675
                     /locus_tag="VBC81_000256"
     CDS             68..1675
                     /locus_tag="VBC81_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6911323.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            1804..2700
                     /locus_tag="VBC81_000257"
     CDS             1804..2700
                     /locus_tag="VBC81_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911324.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            2687..3427
                     /locus_tag="VBC81_000258"
     CDS             2687..3427
                     /locus_tag="VBC81_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911325.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            3424..4509
                     /locus_tag="VBC81_000259"
     CDS             3424..4509
                     /locus_tag="VBC81_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6911326.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            4511..5101
                     /locus_tag="VBC81_000260"
     CDS             4511..5101
                     /locus_tag="VBC81_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911327.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            5151..5855
                     /locus_tag="VBC81_000261"
     CDS             5151..5855
                     /locus_tag="VBC81_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6911328.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            6022..6651
                     /gene="gmk"
                     /locus_tag="VBC81_000262"
     CDS             6022..6651
                     /gene="gmk"
                     /locus_tag="VBC81_000262"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6911329.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            6674..6988
                     /locus_tag="VBC81_000263"
     CDS             6674..6988
                     /locus_tag="VBC81_000263"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6911330.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            7062..9452
                     /locus_tag="VBC81_000264"
     CDS             7062..9452
                     /locus_tag="VBC81_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6911331.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            9499..10434
                     /locus_tag="VBC81_000265"
     CDS             9499..10434
                     /locus_tag="VBC81_000265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6911332.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            10424..11746
                     /gene="rsmB"
                     /locus_tag="VBC81_000266"
     CDS             10424..11746
                     /gene="rsmB"
                     /locus_tag="VBC81_000266"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6911333.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            11784..12521
                     /locus_tag="VBC81_000267"
     CDS             11784..12521
                     /locus_tag="VBC81_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6911334.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            12521..14476
                     /gene="pknB"
                     /locus_tag="VBC81_000268"
     CDS             12521..14476
                     /gene="pknB"
                     /locus_tag="VBC81_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6911335.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            14635..15330
                     /locus_tag="VBC81_000269"
     CDS             14635..15330
                     /locus_tag="VBC81_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6911336.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            15327..16346
                     /locus_tag="VBC81_000270"
     CDS             15327..16346
                     /locus_tag="VBC81_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6911337.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            16339..16980
                     /locus_tag="VBC81_000271"
     CDS             16339..16980
                     /locus_tag="VBC81_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911338.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            17022..18422
                     /locus_tag="VBC81_000272"
     CDS             17022..18422
                     /locus_tag="VBC81_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6911339.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            18424..18780
                     /locus_tag="VBC81_000273"
     CDS             18424..18780
                     /locus_tag="VBC81_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6911340.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(18826..19602)
                     /locus_tag="VBC81_000274"
     CDS             complement(18826..19602)
                     /locus_tag="VBC81_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6911341.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            19723..20478
                     /locus_tag="VBC81_000275"
     CDS             19723..20478
                     /locus_tag="VBC81_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6911342.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            20597..21580
                     /locus_tag="VBC81_000276"
     CDS             20597..21580
                     /locus_tag="VBC81_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6911343.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQEDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            21784..22107
                     /locus_tag="VBC81_000277"
     CDS             21784..22107
                     /locus_tag="VBC81_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6911344.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            22124..22444
                     /locus_tag="VBC81_000278"
     CDS             22124..22444
                     /locus_tag="VBC81_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6911345.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            22446..23747
                     /locus_tag="VBC81_000279"
     CDS             22446..23747
                     /locus_tag="VBC81_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6911346.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            23928..26384
                     /locus_tag="VBC81_000280"
     CDS             23928..26384
                     /locus_tag="VBC81_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6911347.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            26394..27062
                     /locus_tag="VBC81_000281"
     CDS             26394..27062
                     /locus_tag="VBC81_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6911348.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            27130..28218
                     /locus_tag="VBC81_000282"
     CDS             27130..28218
                     /locus_tag="VBC81_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6911349.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(28370..29296)
                     /gene="cysK"
                     /locus_tag="VBC81_000283"
     CDS             complement(28370..29296)
                     /gene="cysK"
                     /locus_tag="VBC81_000283"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6911350.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(29387..30031)
                     /locus_tag="VBC81_000284"
     CDS             complement(29387..30031)
                     /locus_tag="VBC81_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6911351.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            30087..31376
                     /locus_tag="VBC81_000285"
     CDS             30087..31376
                     /locus_tag="VBC81_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6911352.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            31665..>31929
                     /locus_tag="VBC81_000286"
     CDS             31665..>31929
                     /locus_tag="VBC81_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6911353.1"
                     /translation="MLKDVFTKEIKAALKRYKGYTIVPVPLSHEGYQNRQFNQVIAFL
                     QSANIPYKNILSKKDGGKQSANNKEERLKQVQQFTLKNEAELRD"
BASE COUNT        10501 a         5114 c         6368 g         9946 t
ORIGIN      
        1 ccttttttat gataaactta aaagtggact ttctatatga aatctagaaa aaaattgagg
       61 tataaacatg tttaatatta tattagcaat ggtttgtgca ctcattggtt tgattattgg
      121 ttatgtggct atttcaatga aaatgaaatc atcaaaagaa gctgcagaac taactctttt
      181 aaatgcagaa caagatgccg ttgatttacg tggtaaagca gaaattgaag cagaacacat
      241 ccgtaaagca gcggaacgtg aaagtaaagc tcaccaaaaa gaattacttt tagaagcaaa
      301 agaagaggct agaaaatatc gtgaagagat cgaaaaggaa tttaaatctg atagacaaga
      361 gttgaaacag atggaggcac gtttaacaga tcgtgcatca tctcttgatc gtaaagatga
      421 aaatctatct aacaaagaaa aaatgttaga tagcaaagaa caaagtctaa ccgataaatc
      481 tagacatatt aatgagcgcg aacaggaaat tgctacttta gaaacaaaaa aagtagaaga
      541 attgtcacgc atcgcagagc ttagtcaaga agaagctaaa gatatcattt tagctgatac
      601 cgaaaaagat ttagctcatg atattgcgac acgtattaaa gaagcagaac gggaagtaaa
      661 agatcgatca aacaagatag caaaagactt gctagcccaa gctatgcaac gtttagctgg
      721 tgaatatgtt actgaacaaa caattacaac tgttcatctc cctgatgata atatgaaggg
      781 acgtatcatt ggtcgtgaag gacgtaatat tcgtactctg gaaagtttga caggtattga
      841 tgttatcata gatgatacgc cagaagtcgt tgtcttatca ggtttcgacc ccattcgtcg
      901 tgaaattgct cgtatgactt tagaatcttt gattcaagat ggacgtatcc atccagctcg
      961 tattgaagag ttggttgaaa aaaatcgttt ggaaatggat caacgtatcc gtgagtatgg
     1021 tgaggcagca gcttatgaga ttggtgcacc aaatctgcat cctgatttga taaaaattat
     1081 gggacgtttg cagttccgta catcatatgg tcaaaatgta cttcgtcatt cagttgaagt
     1141 tggtaaactt gcaggtattt tagctggaga actaggagaa aatgttgatt tagctcgtcg
     1201 ggctggcttc ctccatgata tggggaaagc tattgatcgc gaagttgaag gcagtcacgt
     1261 tgaaattggt atggaatttg ctcgtaagta caaagagcat ccgattgttg ttaatactat
     1321 tgcaagccac catggtgatg tagaacctga cagcgttatt gcagtaattg ttgctgctgc
     1381 agacgccctt agttcagcac gaccaggtgc gcgtaatgag tcaatggaaa attatatcaa
     1441 acgtctgcgt gatttagaag aaattgctaa tggctttgag ggagtgcaaa atgcctttgc
     1501 acttcaagcc ggacgtgaaa ttcgtatcat ggtacaacct ggtaaagttt ctgatgatca
     1561 agtagttatt atgtcgcata aggtacgtga aaaaatcgaa caaaacttgg attatccagg
     1621 taatattaag gtaacagtta ttcgtgaaat gcgagctgtt gattttgcaa aatagtcaaa
     1681 aggagagctt gttagctctc cttttgttat agagttgaca tttgtcatat tttgaaatga
     1741 caaaaatatc taaaaagttt ccttctgatt ttttatacta tgattatagt aaaggagaac
     1801 caaatggcaa aagatataat taaggttgat catatattta aaagcatcgg gcaaaaaacg
     1861 attttagaag atatatcatt tagtatagct tctaaccaat gtgtagcatt gattggacct
     1921 aatggtgcag ggaaaacaac tctgatgtca actttattag gtgatattag tatttcttca
     1981 ggagcattga ctatttttaa tctgcctgca caccacaata ggttaaaata taaggtggcc
     2041 atcttaccac aagaaaatgt attgccctca aagtttacag tgagagaact cattgatttc
     2101 caaaggtgtt tatttccaga agtgctgcca atgtctctga ttttagacta tctacagtgg
     2161 agtgatgcgc acttgcaaca atttacagag actttatctg gagggcaaaa acgactttta
     2221 gcttttgttt taactttggt tggtaagccc caattactat ttttagatga acctacttcc
     2281 ggaatggata cttccacacg tcaacgattt tgggagctgg ttgcgacact aaaaaaagaa
     2341 ggtgacacaa ttgtctattc tagtcattat atcgaagagg tagaacatac agctgatagg
     2401 attttagtac ttcataaagg aaagttatta cgcgatacaa ccccctttgc catgaagcaa
     2461 gaaaaaaccg aaaagttatt caccgttccg cttagttatc aaaaattatt acctacctat
     2521 ttgattacag agtgtgaagc caagagtgat agtataacgt ttgttactgg ggaggctgaa
     2581 actgtatgga aaatactggc agataatggt tgtcctattg aagctattga gatgaccaat
     2641 agaactttgt taaatcgtat ttttgagact actaaggagg taaaacatga gaatctttaa
     2701 agcattattg aaaatggaat ttcttttgac aaaacgtcaa cttgccaatc tcattatggc
     2761 tataggtatg ccagtggctt tttttctatt tttttcagga tttatgggcg aaggactcac
     2821 taaagccatc gaagctatct acgttcgtaa ttatatgata acgatggcag gattctcaag
     2881 tcttagcttt gcttttttta cttttccatt ttctatgaaa gatgatcaac tctcaaaccg
     2941 aatgcaatta ttaagacact caccagtacc aatgtggcaa tattatctag caaaaattat
     3001 tcgtatttta ttttactatt gcctagcaat aactgttgtt ttcctaacag ggcatatttt
     3061 aaggcaagtt agtatgccaa tagaacaatg gatgcagagc tttttattgc tattaggagg
     3121 agcaacatgt tttattcctt ttggattgct cgtctcctac tttaaaaata ctgaactaat
     3181 gtcaatggta gccaatattt gttatatgtc attagctgtt cttggtggca tgtggatgcc
     3241 aattacaatg ttcccaaaat ggttgcaagg tttatcaaag ttaaccccta cttaccattt
     3301 gactcaagtt atccttagtc cgtttgctaa tagttttgct gttttttctc ttattattct
     3361 gataggctat ggtattataa tgttagttat cgcttatcta ctatctcaga agaggcattc
     3421 aatttgaaaa ataaaaaaaa taactatgat ttaatgtatt ttattccgtt agtatttttg
     3481 atttacccca taggagggat tctctattat cactaccctt tttggacttt attttttacg
     3541 ctagcttttg taggagcata tttatactct attattatca ggggcgaatc aaagtatcac
     3601 atgattgcct ggtttacaat gttgacatat attttctaca tgacagtttt tataaatagt
     3661 ggtttcatct ggtatattta ctttctgagt aatttactcg tctatcgctt tagagataaa
     3721 ttgaagtctt ttagattcat tagttttgct tgcactctag ccacaatggt atttttatgc
     3781 ttctttaagg catcagactt tggtgataga atcatgtttc taattgttcc tattttttgt
     3841 atcggttaca tgtggattgc tattgaaaat agaaatagcg aagaacaaag agaaaaaatt
     3901 gcagagcaaa atcagtatat caatattttg tcagctgaaa atgaacgtaa ccgtataggg
     3961 cgtgatttgc atgatagttt aggacataca tttgctatga tgacactaaa gacagaacta
     4021 gcgttaaagt tgttagaaaa aaggaactat gataaggtac aaaaagaact atcagaatta
     4081 aatcatatta gtcatcaatc gatgagtgag gtccgtcaaa ttgtttctaa cctcaagtat
     4141 cgtacagtag tggaagaaat tgatgagttg taccgcttgt ttcaattatc taacatcaag
     4201 ttaacagttg ttaataagtt agagacgtca caactaagtc cagtaacaca atctacaatc
     4261 acaatgatat taaaggaatt atccaataat attgttaagc atgctgaagc tgatagcgtg
     4321 gagttgagtt tagtaaggca aggtgctact ataaatattg agatgattga taatggttgt
     4381 ggttttacaa accttgacgg agatgagttg catagtattc aagagagatt aactatcgtt
     4441 gaagggacac taacgatttt gtcccgaagt aaaccaactc atattcaggt gatattgaaa
     4501 gaggtataag atgaaattac ttgttgctga ggatcagtcc atgctacgag acgctatgtg
     4561 ccaattactt ttgatggaag aaagtgtttc tactattgat caagctggaa atggtgaaga
     4621 agctattgcc attttatcca ataaagccat agatgttgca atattggatg ttgagatgcc
     4681 gatattatca ggtttagatg tcttagagtg ggttagaaaa taccagaatg ttaaggtcat
     4741 aattgtaaca acctttaagc ggtcaggtta tttccaaagg gctattcgtt caaatgtaga
     4801 tgcttatgtt ttgaaagacc gctcagtagc tgatttgatg aaaacgattc aaaaagtttt
     4861 atctgggggt aaagaatatt cgcctgaatt aatggaaaac gttatatcta atcctttgag
     4921 taagcaagaa ataaaaattc tttccctaat tgctcaaggg aagaccagca aagaaattgc
     4981 atgcacttta tttttatcga atggtacagt tcgtaattac acgtcaaata tttttgataa
     5041 attaggagtt aataatcgtt tggaggcact taaaatagca cgagaaaagg attggctgtg
     5101 atagtttttt tccagtcgag atgccataat gtattaagag ggatatatgt atgcaaaaat
     5161 ttaaagaacg ttttgatacg cttagtgatg caattttggc cattgctatg acgattttag
     5221 ttttggagat aaaaactccg actacgatgg gtgatattgg tgactttaca cggaacatag
     5281 gtttatttat tgttagcttt gttgttgttt ttaatttttg gtacgaaagg gcacaaaata
     5341 gcctagatgc tcaaaagact aatgatgaaa ttatagctct tgatatcatt gaacacctag
     5401 gtatttgttt gattcctctc tttactaaat ttatgatttc ttttgagaat cataattttg
     5461 ctgttatggc ttatggactc ttaacgttat tagttggtct cacatcagac attattcgga
     5521 ttcgtttggc aagctatgat ttagtgacca ttcctagtga attgaaagag cgggttataa
     5581 aagtcatgac aacttttgcc attcgttcag ttgtggttcg ctttatcatt attatcctcg
     5641 cttattttct acctgaggta gggatatttg catatttagt cataccgctc tttatgtttt
     5701 ggcgtcgctc tagagcagga aaatactata ctaaaaaggg cattgaggca ccctcttata
     5761 tcaagctttt tatccaaatt ggagagagag ttcctgagga ttttactaat tcaagtagtg
     5821 gaataagtgg ggtaaaaaga ctctataaaa aataatagga gaccttgaaa aaaaggtttc
     5881 tttttgttaa actaatgaga agaaacttta tttagggagc aatttatgaa ttgctataaa
     5941 agttaatagt taaggtaaga accagattgt tctggtcttt ttaacactta acacccgatc
     6001 tattaatttg aggagtgtgt aatgtctgaa cgtggtttgc taattgtttt ctcaggacca
     6061 tctggtgttg gtaaggggac agttaggcaa gaaatttttt caacccctga ccataaattt
     6121 gattattccg tttcaatgac tactcgtcca caacgcccag gcgaagttga tggagttgat
     6181 tatttcttcc gtacacgtga agagtttgaa gcgttgatta aagaaggtca aatgcttgag
     6241 tatgcggagt atgttggtaa ctactatgga actcctttaa gttatgtcaa cgagacttta
     6301 gacaaaggga ttgatgtttt tcttgaaata gaagttcagg gagcgcttca agttaaatcg
     6361 aaggttccgg acggtgtttt cattttctta acaccaccag atttagagga attagaagag
     6421 cgtttagtag gacgtgggac agatagtcca gaagtgattg cccaacgtat tgaacgtgcc
     6481 aaagaagaaa ttgctttgat gagggagtat gactatgctg ttgtcaatga tcaagtgtca
     6541 ttagcagcag aacgtgttaa acgtgtcatt gaagcagaac attatcgtgt ggatcgtgtt
     6601 attgggcgtt ataccaacat ggtcaaagaa actgataaaa aattaagtta aaaatagaaa
     6661 agagaattgc cttatgatgt taaaaccttc tatcgacact ttgttggata aagttccttc
     6721 aaaatattct ttagttattt tacaagctaa gcgtgctcat gaattggaag ctggtgagaa
     6781 agccactcaa gattttaaat ctgtgaaatc aacacttcgc gctttagaag aaatcgagtc
     6841 aggtaatgtt gtaattcacc cagatccatc agcaaaacgt gcctcagttc gcgctcgtat
     6901 cgaagctgaa cgccttgcta aagaagaaga agaacgtaaa atcaaagagc aaattgctaa
     6961 agaaaaagaa gacggcgaaa aaatttaagc aagtcaaaag tgagaggttg aaacattctg
     7021 tatcagcctc ttttcatatt ttagagaatt agggagggtt tatggcaaga aaattagcac
     7081 aggttattgt tgatattcct ctgatgcaga cagataaacc attttcgtat gccattccta
     7141 aagatttgga agatttggta caagtaggag cacgcgttca tgttcctttt ggaagaggga
     7201 atcgtctttt gcaaggcttt gttgtcggtt tcagagatga tgatgaatta gagactaagg
     7261 atattgcggg ggtcctagat tttgaacctg ttttaaatca ggaacaacta gacttagctg
     7321 atcagatgcg ccatactgtc ttttcttata aaatatctat tttgaaatca atgttaccaa
     7381 gtcttttgaa ttcgcaatat gataaactct tgttagcaac agacacatta ccatcagaag
     7441 atagagaaga tctatttggt cataaaacgg aaattgtctt ttcaagtttg tcttctcaag
     7501 atgccaaaaa agcaggcaga ttaattcaaa aaggtttcat agaagttcaa tatttagcca
     7561 aagataagaa aacaataaaa acagaaaaga tttacaaaat caacagaact ctgttagaag
     7621 agagtcaaat agcagcaaga gcgaaaaaga gactggagtt gaaagaattc ctgttagaaa
     7681 atcctcaacc agggcgcctc acagctttaa acaaacaatt ttcatctcct gtagttaatt
     7741 ttttcagaga agaaggaatc attgaggtca ttgaaaaaga agctagtaga tctgataatt
     7801 attttaaagg aatcctaaaa acggattttt tggatttaaa tcaggaacaa gctaaagtag
     7861 ttaaaatagt tgttgaccga attggtaagg atcagaataa acccttcttg ttggagggga
     7921 ttacagggtc tggtaagaca gaggtatatc ttcatgtcat tgataacgtt ttaaagttgg
     7981 gaaaaacagc gattgttctc gttccagaaa tttctttaac tccccagatg acgaatcgtt
     8041 ttatttcccg ttttggaaag cgagttgcta ttatgcactc gggcttgtca gatggtgaaa
     8101 aatttgatga gtggcgaaag ataaaatcag gtcaagctaa agttgtcgtt ggagctcgtt
     8161 cagcaatttt tgctcctttg gaaaatatcg gagccatcat tatagatgaa gaacatgaat
     8221 caacttataa gcaagaatct aacccaaggt atcatgcgcg cgatgtggcc ttgctacgtg
     8281 cagaatatca taaagctgtg ttactaatgg gatcagcaac tccaagtatc gaaagccgag
     8341 ctagagctag tcgggatgtt tataaatttt tagaattaaa acatcgtgct aatcctaaag
     8401 cacgtattcc tcaggttgaa attattgatt ttagaaattt tattggtcaa caagaagtta
     8461 gtaatttcac accatattta ctagataaaa tccgagatag attagataag aaagagcaag
     8521 tggttcttat gctcaatcgt agaggttatt ctagttttat tatgtgtcga aactgcggct
     8581 acgttgatca atgtcctaat tgtgacattt ctctgacttt acatatggct acaaagacca
     8641 tgaattgtca ttattgtggg tttgaaaagc ctattccaag aacatgtcca aattgcaata
     8701 gtaaaagtat ctcttactat ggtactggaa cccaaaaagc ttatgaagaa cttttgaaag
     8761 ttatcccaga cgcaaagatt ttacggatgg atgttgatac gacaaggcaa aaaggcggtc
     8821 atgagtctat tttgaagcgt ttcggaaatc atgaagctga tattttattg ggaacacaga
     8881 tgattgctaa gggattggat tttccaaatg ttactttagt tggagtacta aatgcagata
     8941 cttctctgaa tttacctgat tttcgatcat cagagcgaac ttttcagcta ttaactcaag
     9001 tggctggacg tgctgggcgt gctgaaaaag aaggagaggt tgtcattcaa acttataatc
     9061 ctaatcatta tgctattcaa ttagcccaaa agcaagattt tgaagctttt tatcaatatg
     9121 aaatgaatat ccgtcgacaa cttggttatc ctccttatta ttttacagtt ggtttaacat
     9181 tatcgcataa agatgaagag tggttaatac gaaaatctta tgaagtatta agtttattga
     9241 agcaaggctt tagtgataaa gtaaagttgt taggtccaac gccaaaacca attgcaagaa
     9301 cacataacct ttatcactac caaattatta tcaagtatcg atttgaggac aaccttgaac
     9361 ttgttttgaa tcgcttatta gacatcactc aagacaagga gaatagagat cttcgcttag
     9421 caatagacca cgaaccgcaa aatatgatgt agaatagtgc tatgatataa ttgaaaaaag
     9481 aaagacgagg aaaaagcaat gacaaaatta ttattcatgg gtacccctga tttttcagcg
     9541 acggttttaa aaggtatttt agcagatggt aagtatgatg ttttagctgt tgtgacgcaa
     9601 ccagatcgtg ctgtaggtcg taaaaaagaa attaaaatga cccctgtaaa agaagtggcc
     9661 ttggaaaaca acattcctgt gtatcagccg gaaaaactat ctggaagtcc agaattagag
     9721 caactaatga ccctaggtgc agatggtatt gtaacagctg cttttggtca attcttacca
     9781 acaaagcttt tggaatcggt aggttttgca attaatgtac atgcttctct attaccaaaa
     9841 tatcgtggtg gggcaccaat tcattatgcc attattaatg gcgaaaaaga agctggggtt
     9901 accatcatgg agatggttgc aaagatggat gcaggggata tggtatcaaa agcttccgtt
     9961 gagataactg atgaagacaa tgtagggaca atgtttgata gactcgccgt tgttggtcgt
    10021 gaccttttat tagatacgct tccaggttat ctatcaggtg atattaagcc gataccacaa
    10081 aatgaagaag aggtaagttt ttctcctaat attagtccag atgaagagag aattgattgg
    10141 aataaatcat cgcgcgacat ttttaaccat gttcgaggga tgtatccatg gccagttgca
    10201 catacccttt tggagggaaa tcgttttaaa ctttatgaag taacaatgtc agaaggaaag
    10261 ggctcaccgg gtcaagtgat cgctaagaca aagaactcat tgacagtagc aactggtgac
    10321 ggagctattg aactcaaatc agttcaacca gcagggaaac ctcgtatgga tataaaagac
    10381 tttttaaatg gtgttggtcg taatttagaa ataggagata aatttggcga atgattggaa
    10441 aaagagtgcg cgtggtttag ctttaatgac tttagaagaa gtttttgata aaggagctta
    10501 ttctaatatc gcacttaata agagtttaaa aaaatctaga ctatcagata aagatcgtgc
    10561 cttagtaaca gaaattgtct atggaactgt ggctagaaaa ataacgctag aatggtatct
    10621 atcacacttt atagttgatc gtgataagct tgaattatgg gtatatcatc tcttgctact
    10681 gagtctttat caactactct atctggacaa catcccagat cacgctattg ttaatgatgc
    10741 tgtcactatt gctaagaatc gaggcaataa aaagggtgct gaaaagttaa ttaatgccgt
    10801 tttacgaaga gtaagttcgg agacgctccc tgaaattgca tcaataaaac gccaaaacaa
    10861 acgttactct gttgcttatt ccatgccagt gtggctcgta aaaaaattaa ttgaccaata
    10921 tggtgagaca agagctttag caattatgga gagtttattt gagcgaaata aagccagtct
    10981 tcgtgtgact gacttatcgc aaaaacaaac tattaaagaa actttaaatg ttagagattc
    11041 acatattgca gagacagctt tagttgcaga ctcaggaaat tttgcatcga catcattttt
    11101 ccaagatggc ctcattacta tccaagatga atctagtcaa ttagttgctc caacactcaa
    11161 agtctcgggt aatgaccaag tattggatgc atgtagtgca ccaggaggaa aaacaagtca
    11221 tatcgcctca tatttgacta caggggcagt aactgcttta gatttgtatg atcataaatt
    11281 ggaattggtt atggaaaatg caaaacgcct aggtctgtca gataagataa aaactaaaaa
    11341 gttagatgca agtaaagctc atgagtattt tcctgaggat acttttgaca aaattttagt
    11401 agacgcacct tgttcaggta tcgggctaat tcgccgtaaa ccagatatta agtacaataa
    11461 agctaatcaa gactttgaag ccctccaaga gattcaattg tctatcctat ctagcgtttg
    11521 tcaaacgcta cgtaaaggtg gtataataac ctatagcact tgtaccattt ttgaggaaga
    11581 aaattttcaa gttattgaaa aattcttaga aaaccacccc aattttgagc aagtagaact
    11641 gagtcataca caagaggata ttgtaaagag aggctgtatt agtataagtc ctgaacaata
    11701 tcatacagat ggctttttta ttggacaagt caaacgtatc ttgtagtgtc taggaggaaa
    11761 ctgacatcgt cagaaaaaaa tatatggaaa tatctctttt aacagatatt gggcaacgtc
    11821 gttccaataa tcaagatttt attaatcaat ttgaaaataa agccggcgtc ccactaatca
    11881 tcttagcaga tggtatggga ggacatcgtg caggaaatat tgctagcgag atgactgtaa
    11941 ctgatctagg aagtgattgg gcggagacag atttttctga gttatcagaa attcgtgatt
    12001 ggatgcttgt ctctattgaa actgagaatc gcaaaatata tgaattaggt cagtcagatg
    12061 attacaaagg tatgggaact accattgaag cagttgccat tgtaggagat aatattattt
    12121 tcgcacatgt aggagattca cgtatcggta tcgttcgtca aggggagtat catttattga
    12181 cgagtgatca ttctcttgtt aatgaattgg taaaagccgg ccaattaaca gaagaagaag
    12241 ctgctagtca tcctcaaaaa aatattatta cgcaatcaat tggtcaagcc aatccagtcg
    12301 aacctgattt aggagttcat ctacttgaag aaggtgacta ccttgtagtt aatagtgatg
    12361 gactaactaa tatgttgagt aatgccgata ttgctacagt actaacacaa gaaaaaacct
    12421 tagatgataa gaatcaagat ttaatcactc tagcaaacca tagaggaggt cttgataata
    12481 tcactgtggc actagtttac gttgaaagtg aggcggttta atgattcaga ttggcaaatt
    12541 atttgctggt cgttatcgga tccttaaatc aatcggacga ggtggaatgg cggatgttta
    12601 tctagctcga gatctaattt tagataatga agaggtcgca ataaaagtat tgcgaaccaa
    12661 ttatcaaaca gatcagattg cggttgctcg cttccagaga gaagcacgag caatggctga
    12721 gctgacacat cctaatattg tcgctattcg tgatataggt gaagaagacg gacagcagtt
    12781 cttagtaatg gaatatgttg atggattcga tttaaaaaag tatattcaag acaatgcacc
    12841 cctatcaaat aatgaagttg ttcgaatcat gaatgaagtc ctttcagcca tgtcactggc
    12901 acatcagaaa ggtatcgtac accgtgattt aaaacctcaa aatattttat taactaaaaa
    12961 agggactgta aaagttacag atttcggtat tgcggtagcg tttgctgaaa ctagtttaac
    13021 gcaaacgaat tctatgttag gtagtgttca ttatttatcg ccagagcagg cgcgtggttc
    13081 aaaagcgact gttcagagtg atatttatgc catgggaatc atgctttttg aaatgttaac
    13141 tggccatatt ccttatgatg gggatagtgc agttactatt gccctacagc acttccaaaa
    13201 accactacca tcaatccttg cagaaaataa aagcgttcca caagctttgg aaaacattgt
    13261 cattaaagca acagctaaaa agctaacaga tcgctataag acaacttatg agatgggacg
    13321 tgatttatca actgctctaa gctcgacacg tcaccgtgaa ccaaaacttg tttttaatga
    13381 tactgagagt actaagacac taccaaaagt aacatctaca gtatcatctt taacgacaga
    13441 acaactgttg cgtaatcaaa aacaggccaa aacaacagaa aaaataactc ctgattctgc
    13501 gagtaacgat aaaacgaagt ctaaaaagaa ggcttctcat cgacttcttg gtacaatcat
    13561 gaaattattc tttgcactct gtgttgtagg aataattgta tttgcttata aaattttagt
    13621 aagtcctacg actattcgtg ttccagatgt tagcaacaaa acagtagctc aagcaaaaat
    13681 gacattggaa aattctggat tgaaagtagg agctattcgt aatattgaga gtgatagtgt
    13741 ttcagaaggc cttgtcgtaa aaacagaccc tgctgcagga cgttctcgta gagaaggggc
    13801 taaagttaat ctttatattg ccactccaaa caagagtttc actctaggta attataagga
    13861 acataattac aaagatatcc tcaaagatct tcaaggcaaa ggtgttaaaa aatctttaat
    13921 caaggttaag cgtaaaatta ataatgatta tactactggt acaatcttag cacagtcgct
    13981 tccagaagga acatctttta atcctgatgg taataagaaa ttaacactta cggttgctgt
    14041 taatgatcca atgattatgc ctgatgtaac aggtatgaca gttggagaag taatagaaac
    14101 cttgacagac ctcggattag atgcagataa tcttgtcttc tatcaaatgc aaaatggcgt
    14161 ttatcaagca gtagtaacac ctccatcttc aagtaagata gcaagtcagg atccttatta
    14221 cggaggagaa gttggattaa ggagaggaga taaagttaag ctgtatcttc ttggttctaa
    14281 aacaacaaat aactcatcat ctacgccaat agattcttcg gcttcaagct caacaggaac
    14341 aactacctcg gattctgtaa gctcatcaac ggatgctagc acttctgata gtagtagcac
    14401 ttctacaagt agttcaacac taccaagtga tagtacaact aatacaggaa cagctaacaa
    14461 tccattaact caataaaatt aaaaagaaga tgttaaagtc ttctttttaa tttgctatgg
    14521 tagagaccat gcctagaaag gttctgccaa tatgccaaat ttttctctca ataatggtgc
    14581 aaagtatagg gctttttgat aaaatacgtc atatagagta atggaaggat tattatgaaa
    14641 aaatttcagt tttttctact tgttgaagcc gttgtcttag ttatgggatt aatgaaaatt
    14701 ttatcagatg actggacaag ttttattttt attctcgctt tgattctttt agccttaaga
    14761 ttttataaca acgatagtcg tcataatttt ctgttaacga caagtttgtt attactattt
    14821 ttaatcttta tgcttaaccc ttacattatt gcagccgttg tttttgcagt tttgtatgtt
    14881 ctaatcaatc atttctcgca agttaagaaa aaaaatcgtt atgctttgat tcaatttaag
    14941 aaccatcaat tagatgttaa gacaacacga aaccaatggt taggtactga tcagcacgaa
    15001 agtgactttt atgcttttga agatattaat attataagaa tctctgggac tgatacaatt
    15061 gatttaacaa atgttattgt atctgggcag gataatgtta ttataattca aaaagttttt
    15121 ggtgatacca aagtattggt tcctttagac gttgccgtaa aggctgatat tagttctgtt
    15181 tacggtagtg tacagtactt tgattttgag gagtatgatc taagaaatga atctataaaa
    15241 ttgtcacaag aggaagaata ttaccttttg aaacgtgtca aattagttgt aaacactatc
    15301 gctgggaaag ttgaggttag tcgtaaatga aaaagcacca ctacttttta gctttcttct
    15361 atggaagtgt tattatattt gccatttgtt ttgtcattat tgatagtttg ggtgttaatt
    15421 tggtgcacct ctatcaaacg tctcgtttgt ggctgattga acaactgatt ttctctattt
    15481 tctttctatc tttagcggta actatccttt tattgctaac ttggttttta ttagacgata
    15541 atagtaaacg tcaaattaac cataatttac gtcgcatttt aaataaccaa tcaataaatg
    15601 ttaccgatga tggtactgaa atatcgacca atattcaacg gctctctaaa aaaatgaatt
    15661 taatgacagc aagtctacaa tctaaggaaa atagtcgtat tttaaaaagc caagaaattg
    15721 tgaaacaaga acgtaagcgt attgcacgtg atttacacga cacagttagt caggatttgt
    15781 tcgcagcttc catggttttg tcagggattg cacagaatgt ttctcagcta gacgttgatc
    15841 aggttggctc gcaattatta gcagtagagg aaatgcttca gcatgcacaa aatgatttga
    15901 gaattctgct acttcatttg aggccagttg aattagaaaa taagacccta tctgaaggat
    15961 tcaaaatgat attgaaagaa ttgaccgata agagcgacat tgaagttgtt tatcacgaat
    16021 caattctaac cttacccaaa aagattgaag acaatatttt tagaattggt caagaattta
    16081 tcagtaatac tttaaagcat tcacaagcca gtcgtctcga agtgtacctc aatcaaacag
    16141 agaatgaatt gcagttaaaa atgattgata atggtattgg atttgatatg gattctgtct
    16201 atgatttaag ttatggatta aaaaatattg aagatcgagt agaagattta gcaggaaatt
    16261 tacagcttct cagtcagcct ggtaaaggag ttgctatgga tataagatta cctttagtaa
    16321 atcagagtga ggataaaaat ggataaaata aaaattgtac ttgttgatga ccatgagatg
    16381 gttcgtctcg gtttaaaaag ttttttaaac ttacaagctg atgttgaagt aataggtgaa
    16441 gcctcaaatg gtttagaagg cataaaaaaa gcactggagt tacgtccaga cgttgttgtt
    16501 atggatttag taatgcctga aatggacggt gtagaagcaa ccttagcatt gttgaaggat
    16561 tggccagaag ctgctatttt agtcttaaca tcttacttgg acaatgaaaa aatttaccct
    16621 gttattgaag caggtgccaa aggctatatg ttaaagacct caagtgcagc agagattctt
    16681 aatgccattc gtaaagttgc aaggggagaa caagctattg aaaatgaagt agataagaaa
    16741 attaaggctc atgataaatg tccagcttta catgagggct taacagcaag ggaacgtgat
    16801 atccttaatt tattagccaa aggttatgac aatcaaagga ttgctgatga attatttatc
    16861 tcgttaaaga ctgttaaaac tcatgtttct aatattttgg gaaaactaaa tgtggcagat
    16921 cgaactcaag cggttgtcta tgctttccaa caccatttgg taccacaaga tgatgaataa
    16981 gtgatataat agagacacta tgttaatgaa agtggttagt catggacgca aaaactaaat
    17041 ataaagcaaa aaaaataaag gcggtgtttt ttgatattga tgacacgctt cgtgttaaag
    17101 atacaggtta tatgccacca tcgatcttaa aagtttttaa agctttgaaa gataaaggaa
    17161 ttgttgtagg tattgcttca ggaagggcac gatatggcgt tccaaaagaa gttcaagatt
    17221 taaatgccga ttattgtgta aaattaaatg gagcttatgt taaggacaag gataagaata
    17281 tcatcttcca tcgtcctatt cctgctgaat atgtagagca atataagaaa tgggcagaca
    17341 ctgttggtat taagtacggt ttagctggtc gtcatgaggc agttttatca gatcgtgatg
    17401 atttggtaaa tgatgcgatt gatattgtgt attctgatct cgaagttaat ccagatttca
    17461 ataaagaaca tgatatttat cagatgtgga cgtttgaaga taaaggagat agccttcatc
    17521 tacctgaacc gttagatgaa catcttcgct tgatacgttg gcatgatcat tcttcagatg
    17581 ttgtgttaaa aggaacatcg aaggctttag gtgtttctaa agttgttgaa catttgggct
    17641 taaagccaga gaatatcttg gttttcggag atgagctcaa tgatttagaa ctctttgatt
    17701 atgctggact agctgtggcg atgggagtgt cacacccaga agctcaaaaa aaagcagatt
    17761 ttatcacaaa aaaagtagaa gaagatggca ttttgtatgc cctggaggaa ctaggtttga
    17821 tagaaaaaga attaacattt ccacaagtag atattgaaaa tacagaaggt cctgtggctg
    17881 tcattaaaac aaatcacggg gatatgactg taaaactctt cccggatcat gctcctaaaa
    17941 cggtagcaaa ctttattggt ttagctaaac aaggttatta tgatggtatt atcttccatc
    18001 gtattattcc agattttatg attcagggtg gtgatccaac tggtacaggt atgggtggtg
    18061 aatctatcta tggggaaagt tttgaagacg aattttccga agaattatat aatgttcgcg
    18121 gagctctttc aatggctaat gctggaccaa atacgaatgg cagccaattt ttcatcgtcc
    18181 aaaatacaaa aattccttat gcgaaaaagg aattagagcg aggtggttgg ccaacaccaa
    18241 ttgctgagtt gtatgctgga caaggtggta caccgcatct agatcgtcgc cacagtgtat
    18301 ttggacaatt agtaaaccaa agttcttttg aggtacttga tgagattgcg gcagtagaga
    18361 ctggttctca agacaaacca ttggaagatg ttgttatttt aacgattgag gttaaagaat
    18421 aaaatgaaga ttggcgataa gttaaaggga acagtcactg gcattaggcc ttatggagcc
    18481 tttgtttctc ttgaggatgg taggactgga cttatccata tttctgaaat taaaacaggt
    18541 tacattgata atatttatga tgttcttagt gtaggtgatg aagtctatgt tcaggttata
    18601 gatgtagatg aatttacaca aaaagcaagt ttatcgcttc ggacccttga agaagaaaga
    18661 catcatattc agcatcgtca tcgattttct aacaatcgtc ttaagatagg tttcaaacca
    18721 cttgaggaaa atttacctag ttgggttgag gaaggtctag cctacttgaa taataaataa
    18781 acaaaaacct agatttgatt aatgacaagt ctaggtttta aatatttaaa agtagcagtg
    18841 aatattatac ttgctaaaaa tagcttgata atcgagtaga tcctctgggt gtagtgcagc
    18901 gatctcttcc atttcatatt gacgattaag taatcgatat ttgttttgtc caaattggtg
    18961 gaaaggtaag agttgcactt gacggatatc taggcgatca aataaacaag caaattcttc
    19021 agcatcttct aatgaatcgt taaaattagg tatgacagga atacgtagca caatcgtttt
    19081 accgttggca aaagcataat gaatgttttt gatgattgaa gcattcttaa ccatcgtctt
    19141 ttcttggtgt ttgaggctat tgtaatgttt taaatcggtg taaataaaat caacgtattg
    19201 gataaggtct atgaattgtt catgtctagt ataagcagtt gtttcaatag ccgtgtggat
    19261 tcccagtgat ttagcacgtt ttaaaattgc cttggcaaat tcaaattgtg caaatatttc
    19321 accgccagat agcgttatgc ccccaccaga ttcttcgtaa aaatcaatat cttttaaaac
    19381 ctcttcaata atatcatcaa ccgatttttc ttcgcccacg atgacgcttt cattagtaat
    19441 ggcatctctc attgtttctg gaaccatttt ttgcgactcg ggattagcac accaaggaca
    19501 tctaagagga caacccttta agaaaacggt tgttcgaata cctggaccat cgtgaatact
    19561 aaaatgttga atgttgaaca caataccttt ttcatttgtc atattatttt cctcaatttc
    19621 attttaagta taccttttat acgtaacaaa gtcaattacg aatgaaagtt tattgaactt
    19681 gtttaataaa taaaaaatga taaaatagaa aggaggtgtt tcatgaatag attagaaaat
    19741 attattagtt tagtatccca gtaccaaaaa atagatgtta atacactctc ggagttgttg
    19801 caagtttcta aagtaaccat acgtaaagat ttagataaac tagaaggcaa agggcttttg
    19861 catagagagc acggttatgc agttttaaac agtggggatg atcttaatgt taggttatct
    19921 tttaaccata aaactaaaaa agaaattgct gccttagctg caaatatggt gtctgacaat
    19981 gacactatct taatagaatc aggctctact tgtgctcttt tagcggaaaa tatctgtcag
    20041 acaaagcgaa atgtcactat tttgaccaac tcttgcttta ttgctaacta tttacgagag
    20101 tatgattcat gccaaattgt cttacttggt ggtgagtatc aatcaagttc acaggttacg
    20161 gtaggtcctc tcttgaaaaa gatgatttca ctttttcatg tctcgttagc ttttgttggg
    20221 actgatggtt ttgatccaaa actaggattt atgggtagag atttaatgcg ttctgaagtt
    20281 gcacaagaga tggcgaatgc tgccgatgaa gtcattattt taacagattc tagtaagttt
    20341 aatcagactg cattggtgga acagcttccg ttgtcaactg tatcacaagt tattacagat
    20401 aaacatccaa attcggatat tgctaacttg ttccaagaag cagagattac cattcaatct
    20461 ttattacaag taccataaaa gaggctattt ttcttacatt ttgcacattt gtggcgataa
    20521 tagataaagt gtcatgtcaa attgcacaga tgtaaccttt ttgttaaact gaagcaaatg
    20581 ctgaaaggtt atgagtatga aattagagcg tcaaaaattg ttagctaaag tagcttacct
    20641 ttactatatg gaagggaaaa gccaatctga gattgccaat gaattaggaa tttatcgtac
    20701 aacaataagt cgtatgttag ccaaagcgag agaagaaggt cttgttagaa tcgaaatttc
    20761 agattttaat ccagaaattt ttcagttaga atcctatttt aaaagtaagt atcaccttaa
    20821 agatattgag attgtttctt cgcgaaaaga tagcgatact tcagagatag agaaggattt
    20881 agcacatgta gctgcagcta tgattcgaaa aaaaattaaa gagaatgaca aggtggggat
    20941 tgcttggggg aggacactta gtaaagtagt tgaagcaatg cgtcctcatc cggtttctca
    21001 agtttctttt gttcctttgg ccggaggtcc aagtcatatt aatgcacgct atcacgttaa
    21061 tactctggtt tacgaaatgt caaggcgttt ccaaggaagt tgtactttca ttaatgcaac
    21121 tctagttcaa gaaaacgcta atttagcgaa aggaatatta acttcaaaat attttgaggg
    21181 gctaatggat aattgggaaa aacttgatgt ggcaattgtg ggcgttggtg gtaaacctaa
    21241 aagcaatgaa caacagtggc ttgatctttt aaatcaagaa gattttcaat gcttggatga
    21301 agaagcagct gtaggtgaaa ttacttgtcg tttttttaat cattcaggtg attctgtgaa
    21361 tcaacattta gctaaaagga caataggaat taccttagaa caactacaga aagttcctaa
    21421 tagaatagcg gttgctcatg gcaattataa ggctgcggca ctcttagccg ttttaaaaaa
    21481 ggggtatatt aatcatttag ttactgattt ttccacagcc ttaaatattt taagattaga
    21541 taaagacacc tttgtggata ctatgtatca aaagagctag gatttattcc tagctctttt
    21601 aataatgttt ctattaccaa gtgaattgtt atagaataag cacaaaagtg cgctattatc
    21661 ataaagggca caaggtctat tgacgacgat taagggacat gttacatttg ttttgcgaaa
    21721 gaaaattaga accttttgta agaaagttta aacttataaa aaatcattaa agcggaggtg
    21781 cttatggaaa tcatagtcgc agaccaaatt attatggggc tcattttgaa tgctggggat
    21841 gctaaacaac atatctatca agccttaaaa cttgcaaaag aaggaaattt tgcagaaagt
    21901 aaaattgaaa tagaactagc agatagtgca ctcctagaag ctcataatct tcaaacacaa
    21961 ttccttgcac aagaagcagg agggacaagg acagatatct cagcactctt tattcattct
    22021 caggatcatt tgatgacaag tattactgag attaacttga ttaaagaaat tattgattta
    22081 cgccaggaat tacatattca aaaataagta aaggggatca aatatgataa aaattggttt
    22141 attctgtgca gcaggttttt caacaggtat gttagtaaac aatatgaaaa tcgcagcgga
    22201 taaagaaggg atagaggcac atattgaagc ttattcacaa ggaaaaattg cagattatgc
    22261 taaagattta gatgttgcat tacttggtcc acaagttagc tatactctag ataaatcaaa
    22321 atcaatctgt gatgaatacg gtgtaccgat agcagttatt ccaatggcag attatggtat
    22381 gttggatggt gtaaaagttt tgaaacttgc tttgagttta ttggaaacaa gaaaagaggc
    22441 ttgatatgtc aaagtttgat agtcagaaaa taattactcc gattatgaag tttgtcaata
    22501 tgcgagggat tattgcactc aaagatggca tgctagcaat tttaccacta acagttgttg
    22561 ggagtctctt tttaatatta gggcagcttc catttaaagg tcttaatcaa gccatagcta
    22621 atgtttttgg accagaatgg acagaaccat ttatgcaagt ttattcagga acttttgcga
    22681 ttatgggctt gatttcctgt tttgcaattg cttatgctta tgctaaaaat agtggtgtag
    22741 aaccgttacc tgctggcgtt ttatcacttt cgtctttctt tattttaatg aaatcatctt
    22801 atattcctgt aaaaggagaa gcaatagcgg acgctatttc taaggtttgg tttggcggtc
    22861 aggggattat tggagcaatt attattggct tagtagttgg tgctatctat acatggttta
    22921 tccaacatca tattgttata aaaatgccag agcaagtacc acaggcaata gcaaaacaat
    22981 ttgaagctat gattccagct tttgttatct ttcttttatc gatgattgtt tatttgattg
    23041 ctaaggtaac aactggaggt acctttattg agatgattta tgatatcatt caagtaccgt
    23101 tgcaaggctt aacaggctca ctttatggag caattgggat tgctttcttt atttcattct
    23161 tatggtggtt tggtgtccat ggccaatctg tggtgaatgg tattgtgact gctttgttac
    23221 tatccaattt agatgccaat aagtctttat tagcagcaaa tcgcttaaca ttagataatg
    23281 gcgctcacat tgtaacacag cagtttttgg atagtttctt gattttatcg ggctcaggaa
    23341 taacttttgg actagttatt gcgatgcttt ttgcagcaaa atcaaaacaa tacaaggcac
    23401 ttgggaaagt ggccgctttt cccgcaattt tcaatgttaa cgagccaatc gtctttggct
    23461 ttccaatcgt aatgaatcct gtgatgtttc tgccttttat tttagtacca gttttggcag
    23521 ctttaattgt ttatggagct attgcggttg gttttatgca accattctca ggtgttactt
    23581 taccatggag tactccagca attatttctg gattcatggt aggtggttgg caaggtgcac
    23641 tcgtccaaat agtcatttta gctatctcaa ctgctgttta cttcccattc tttaaaatcc
    23701 aagataatat tacttacaaa aatgaatgtg aaatggaaag gggatagcta gcagataaga
    23761 ggttgaggtg tgtaatgcac cttaaccttt ttctaaaaat gcacatttgt gcaactttag
    23821 atgtagcatt ctattcttta ttttcttctt gttattttag gatttaggtg ctattatatt
    23881 tacgaagaaa aactttttag tttcgaaaga aacaagcgga ggctgttatg accaaacaat
    23941 tactcactca aaaatataca aatgatgttc aacaaaattc tcaaaagcat tttggctatt
    24001 tgacagagcg catgtattct tatcgcgata aggttcttga taagaaacca ttcatagatg
    24061 cagaacgtgc tatcttggta actgaagcct atcaaaagca tcaggaaaaa cctaatgttt
    24121 taaagagagc ttacatgttg caaaatattt tggaaaaaat gacaatctat attgacgatg
    24181 agacaatgat tgtcggtaac caagcttcat ctgataaaga tgctcctatt tttccagaat
    24241 acactctaga atttgttgta aatgaactag acctttttga aaaacgagat ggtgacgtct
    24301 tttacattac agaagaaacc aaagaacaaa ttagaaatat tgcacctttc tgggaaaata
    24361 ataatttacg ggctcgtgcg ggcgttatgc ttccagaaga agttcaagtt tatatggaga
    24421 caggcttctt tgggatggaa ggtaagatga attctgggga tgctcactta gctgttaact
    24481 atcagaagtt actagaagaa gggcttattg gctttgaaaa aaaagcaaga aaagctaaag
    24541 ctgacttaga tttaacaaag cctgagagta ttgacaaata tcatttctat gattctattt
    24601 taattacaat agaagctgtt aaaacctatg cagagcgttt tgctatccta gcaaaaaaac
    24661 aagctaaaac agcaaatact aagagacgtc aggaattgtt agatatcgct agtatttgtg
    24721 aacgagtgcc atattaccca gctgaaacgt ttgcagaagc tgttcaatcg gtttggttta
    24781 ttcagtgtat tcttcaaata gaatcaaacg gtcattctct atcttatgga cgctttgatc
    24841 aatacatgta tccatatgtt aaagctgact tagaagctgg acgtgagact gaggattcga
    24901 tcgttgagcg cctaactaac ctatggatta aaacaataac tattaataag gttcgcagcc
    24961 aagctcatac gttttcgtct gctggtagtc ctctttacca aaatgtaacg attggaggac
    25021 aaacccgaca taaggaagat gctgttaatc ctttatcatt tctagtacta aaatcagtcg
    25081 ctcagacaca cttaccacag cctaatctaa cggttcgtta tcatgctaac ctagacaagt
    25141 cattcatgaa cgaagctatt gaagttatga aattaggttt tggtatgcca gcttttaata
    25201 atgatgaaat tattattcca tcatttatta aaaaaggagt ttccgaagaa gatgcctatg
    25261 attattcagc gattggttgt gttgaaacag cagttcctgg gaaatggggt tatcgttgta
    25321 ccggcatgag ctatatcaac ttccctaaag tgttattaat tacaatgaat aatggcattg
    25381 acccggcttc tgggaagaga tttgcgcctt cttatgggca tttcacccag atgacctcat
    25441 ataaagaact taaagaagct tgggacaaaa cacttcgtta cttaactcga atgagtgtta
    25501 ttgttgaaaa tgctattgat attagtctcg aaagagaagt acctgatatt ctttgttcag
    25561 ctttaactga tgattgtatt ggacgaggta agcatttaaa agaaggtggt gcagtatatg
    25621 actacatatc tggtctacaa gttggaatcg ctaatctttc agactcactt gctgcgctta
    25681 aaaaattagt atttgaagaa aaacgcctaa ccacattaga agtatggcaa gcattgcaga
    25741 gtgattacgc aggtccgcgt ggtgaagaaa ttcgtcaaat gttgatcaat gaagcaccaa
    25801 aatatggaaa tgatgatgac tacgcggata gtttagtacg tgagtgttat gatgtttatg
    25861 ttgaagaaat tgctaaatat cccaacacac gctatggacg tggaccaatc ggtggtattc
    25921 gctattcagg aacgtcctct atttcagcaa atgtaggtca aggacgaggt actttagcaa
    25981 ctccagatgg tagacatgca ggaactccat tagctgaagg ttgttcacca tctcataata
    26041 tggacaaaaa gggacctacc tctgttttga aatcagtttc gaagttacca actgatgaga
    26101 ttgtcggcgg tgtactcctt aaccaaaaag tcaaccctca aaccttggca aaagaagaag
    26161 ataaacaaaa attaattgcc ttacttagaa cattcttcaa tcgccttcat ggttaccata
    26221 tccagtataa tgttgtctca cgtgagactt tgattgatgc tcagaagcac cctgaaaaac
    26281 accgcgattt gattgtgcgt gtagcaggtt attctgcatt ctttaatgtc ctatctaagg
    26341 caactcaaga tgatattatt gcacgtacag aacatgctct ttaggagatt attatggaat
    26401 ttttacttga cactttaaat ttagaagcta taaaaaaatg gcatcatata ctgccactag
    26461 caggtgtaac ttcaaatccc accattgcta agaaagaagg agatatacat tttttccaac
    26521 gcattcgtga tgttcgagaa attattggat gtgaagcaag tttgcatgtt caagtagtgg
    26581 ccaaagatta tcaagggata ctggatgatg ctgctaaaat acgccaagaa acggatgatg
    26641 acatttatat caaggttcca gttacgccag atggattagc agcaattaaa acgttaaaag
    26701 cagaaggtta taatattaca gccacagcaa tatacaccag tatgcaaggt cttttggcta
    26761 tatcagctgg agcagactac ctagcacctt actttaaccg aatggagaac cttgatattg
    26821 atgcgacaca agttattaag gaattagcgc aagcaataga gaggacaggt tcttctagca
    26881 aaattttagc agctagtttt aaaaacgctt cacaagtaac gaaggcctta tcacagggtg
    26941 ctcaatcaat caccgcggga ccagatattt ttgaatcagt ctttgcgatg ccatctattg
    27001 caaaagctgt taacgacttt gcagatgatt ggaaagccag tcaacactca gaacatattt
    27061 aacagagaac gccaactttt gaggctgtat ctttaacata aaatttttta aagaagaaag
    27121 aggttgagaa tgaaagtttt cgcaagtcca tcgcgctata tacaaggaaa ggatgcttta
    27181 ttccaaagta ttgaacatat caaatcttta ggtcagactc cacttatcct ttgtgatgat
    27241 gttgtttata atatcgttgg agaaaggttt ttatcttatc tacaagacga tttgttacca
    27301 catcgtgtct cctttaacgg agaagcatct gataatgaaa tcaatcgtgt cgttgctgtt
    27361 gcaaaagaaa agaatagtga ccttattatt ggacttgggg gtggaaaaac tattgactct
    27421 gcaaaggcaa ttgcagataa agttaattta ccagtgatca ttgcaccaac agtagcttca
    27481 acagatgccc caacatcagc gttatcagta atctatacag atgaaggagc ttttgaaaaa
    27541 tatatttttt attcaaagaa tccagactta gttttggtcg atactcaagt cattgctcaa
    27601 gcgccaaaac gtttactagc ttctgggatt gcagatggct tagctacttg ggttgaagcg
    27661 agagcagtct tacaaaaaaa tgggatagcg atggctggcg gtagacagac tttagcgggc
    27721 gtggcaattg ctcaagcatg tgagcgtact ttatttaatg atagtttaca agctttagcg
    27781 gcttgtgatg tcaaagtggt tacaaaagct ttagaaaatg ttattgaagc taatacctta
    27841 cttagtggtt taggttttga aagcgcagga ttagctgcag ctcatgctat tcataatggt
    27901 tttactgcct tgagtggaga tattcatcac ttaacccatg gtgaaaaagt tgcctatgga
    27961 acgttgacac aacttttcct cgaaaataga ccaaaggaag aaattgatcg ctacattaat
    28021 ctttaccaag cgattggaat gccaactaca cttgcagaac tccatttggg tgatgcaaca
    28081 tatgaagaac ttttaaaagt tggtcagcaa gcaacaattg aaggtgaaac aatacatgaa
    28141 atgccattca aaattagtgc agaggatgtc gctgcagccc tgttagcagt tgaccgctat
    28201 gtttcaaatc atcaataaaa acaatagaac caagtgaaat tgcttggttc tatttgtcgt
    28261 tttaatattt taactaaata gggcttacta gtatttaaag agatataaac taaaaaagat
    28321 cagacaacat atctaatctt ttttagttat cgtttactga agattaaagt tattcaaaat
    28381 catacaggct tgttgataag taacgttcgc cattatcagg gagaagagct aaaacctttt
    28441 tcccttttcc tagttgtttg gccacttcaa tagcagcata aatagcagct gaagcagaaa
    28501 ttcctgcgag gaaaccttct agtccgccga tgatacgtcc tgtttcaata gcatcgttag
    28561 atgtaacgcg aatgatatga tcataagaat ctgtagccaa tgtttctgga atgaaacctg
    28621 cagaaatccc ttgtatttta tgaggcccag gttgctcacc agaaaggatt gcagattcat
    28681 cagcttctac agcataaata gcgacatcag gattgacttt ttttaatgca cgagagacac
    28741 cagtaattgt cccaccagtt cccacacctg cgataaaagc atctaaacct ttttcaccaa
    28801 aagtctcgat aatttcccta ccagtcgttt tttcgtggat ttctggattg gcttgattat
    28861 taaattgcag gggtagccaa gcattttgct ctgcagaaat ttcttttgcc ttagcaatag
    28921 ctcccttcat cccttcacta ccaggtgtta gtactaattg agcaccatag gcttgaatga
    28981 ttttacgacg ttcaattgac atggtttctg gcataacaat aataacatta taccctttag
    29041 cttttcctac ccaagccaaa ccgatacctg tattaccaga tgtaggttct acgattgtat
    29101 ctccggcttt aagaatccct ttttgctctg catcttcaat catagctaga gcgatacgat
    29161 ctttgacaga tgaacctggg ttaaaggatt ctaacttaac gtacacctct gcagctcctt
    29221 ctggaacaat atgatgtaat tgaataatgg gtgtatttcc gattaagtct gtaatagaat
    29281 tataaatttt tgacataagc tatacctctt catgacattg gttaagaata ttataaaact
    29341 cacattgatt aaagtaaaat atcaattctt tatcaagtga tagctattat ttatcggttt
    29401 cagccttaaa gggaatttca acaatttgac tcccttcttc agttaacaaa gcttttcctt
    29461 ggtagaactc tgtcaaatta gttattgtgt gttcaaattc cttagggtca acgtatattt
    29521 tacaggttac agcttctaaa aattctgttt caaactcctg aaggtggtgc tcttttagga
    29581 agttgtcaaa agtttgatat tgaggataag ttaattgaat acgaatacca atttgttctt
    29641 taacttctac taccccgatc tctttgatgg cattagctac gctaccagag taggcacgaa
    29701 taagcccccc tgtgcctaat ttgatacctc caaaataccg tgtaacgacg gcaacaacat
    29761 ttgtcaggcc ttgcttttca agtacggtta gcatagggat acctgctgta ccactaggtt
    29821 caccatcatc actagatcgt tttatatccc ctttttcgcc aataaccata gctgaacacg
    29881 aatgattggc tttgtagtgc gctttcttga tttgcgtaat ataattacgc ccctcctctt
    29941 cggactctac tcgctttaga tgacaaataa atcgcgattt tttaatttct tcttcgacaa
    30001 ttccgtctga ttttatcgtt ttatagttca tagagaaatt ttacaaaaat tcttcaaatt
    30061 tagcaaaaaa atccgaatga aataatatgg aaaattattt aggtagactt tggacaaaag
    30121 cacaattatc tgagcaactt agaaaaattg caattaactt gccatctttt ataaagaaag
    30181 gaagcgacta tatttgtact cgatgctcta gttctgttgc caaaaattgc caattaccaa
    30241 ctggaaatta ttattgtaga gaatgtatag tttttggaag agttactagt aatgaaaatt
    30301 tatattattt cccacaaaaa acattttcta aaacgaattc tttaaaatgg aaagggggac
    30361 tgacacctta tcaaaatgag gtttcagaag agttgttgaa aggtatttca agtaaggaaa
    30421 atcttcttgt tcatgctgtg acaggggctg gtaaaacaga aatgatatac cactctatcg
    30481 ccaaagtaat tgatactgga gggagcgttt gtattgccag tcctagaatt gatgtttgtt
    30541 tagaattgca taagcgactt tcaaacgatt ttagatgtgc cataacatta atgcatggag
    30601 aatctccttc ttatcagaga tcccctttaa ctattgccac aacacatcaa ttgttaaaat
    30661 tttatcatgc ttttgatttg ctaattgttg atgaagttga tgcttttcca tatgtcgaca
    30721 acccgatact ataccatggt gtaaaacagg ccttaaagaa aaacggaaca agtatttttc
    30781 tgactgcaac ttcaacgact gagttagaaa gaaaagttgc aagaaaagaa ctaaaaaaat
    30841 tacatctagc taggagattt catgccaatc cgttagtgat acccgagatg gtttgggtaa
    30901 gtggtatcca gaaaagcttg cagacacaaa aactacctcc caaattatat cagctgataa
    30961 ataaacagcg acaaacgaga tatcctttgt tattattttt cccccatatt gcagaaggtc
    31021 aggtattcac cgaaatgtta agacaagcct ttcctatgga aaaaattggt tttgtttcaa
    31081 gtaagagcac atctaggtta aagttagtgc aagattttcg agataacaaa ttgtctatcc
    31141 tagtatcaac taccattcta gagagaggag taactttccc tagcgtcgac gtttttgtta
    31201 tacaagcaaa tcatcatttg tttacgaaaa gttccttggt acaaatttca ggaagagttg
    31261 gaagagcatt agaaagaccc gaagggctcc tttacttcct tcatgatggg aaaagtaaat
    31321 cgatgcacca agccataaag gagattaaaa acatgaatca tataggagga ttttaatgac
    31381 ttgtttgtta tgtcatgaaa tagatttatc gcaattaact tttgtggaac ttatacttct
    31441 aaaaccaaag caaaacgtaa tttgtcaaac gtgcaagggt agttttgaag cacttagtag
    31501 ggagatgggt tgccaaacat gttgcaagca aatcctccga aaacaatgtc aagattgtat
    31561 ttattggggt aaaaaaggta tagaggtaaa tcatttttcc ctttatagat acaatgaagc
    31621 aatgaaaaaa atatttttct ctttttaaat ttcaagggga ttatttgttg aaagatgttt
    31681 ttacaaaaga aataaaggct gctctaaaaa ggtataaggg ctacactata gtgccagttc
    31741 ccttgagtca tgaaggatac caaaataggc aatttaatca agtgattgct tttctacaat
    31801 cggcaaatat accttataaa aatattcttt ctaaaaaaga tggaggtaag caatcggcta
    31861 ataataaaga agaaagactc aaacaagttc agcaatttac attaaaaaat gaggctgagt
    31921 taagggata
//
LOCUS       DATRNX010000007        31260 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRNX010000007 DATRNX010000000
VERSION     DATRNX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31260
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            10..387
                     /locus_tag="VBC81_000287"
     CDS             10..387
                     /locus_tag="VBC81_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911354.1"
                     /translation="MQLAKTEINKGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNI
                     AIVIFCIKNLRAIKRNQMVNYLPYYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIF
                     GLLYFHAYQKAQKLNERDLEETN"
     gene            complement(458..1567)
                     /gene="dinB"
                     /locus_tag="VBC81_000288"
     CDS             complement(458..1567)
                     /gene="dinB"
                     /locus_tag="VBC81_000288"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6911355.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            1737..4049
                     /gene="pflB"
                     /locus_tag="VBC81_000289"
     CDS             1737..4049
                     /gene="pflB"
                     /locus_tag="VBC81_000289"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6911356.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            4150..4523
                     /locus_tag="VBC81_000290"
                     /pseudo
     CDS             4150..4523
                     /locus_tag="VBC81_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(4558..5487)
                     /locus_tag="VBC81_000291"
     CDS             complement(4558..5487)
                     /locus_tag="VBC81_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6911357.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(5484..6239)
                     /locus_tag="VBC81_000292"
     CDS             complement(5484..6239)
                     /locus_tag="VBC81_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6911358.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            6364..7260
                     /locus_tag="VBC81_000293"
     CDS             6364..7260
                     /locus_tag="VBC81_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6911359.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(7339..8187)
                     /locus_tag="VBC81_000294"
     CDS             complement(7339..8187)
                     /locus_tag="VBC81_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6911360.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGSTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(8355..8807)
                     /locus_tag="VBC81_000295"
     CDS             complement(8355..8807)
                     /locus_tag="VBC81_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6911361.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(8825..10027)
                     /locus_tag="VBC81_000296"
     CDS             complement(8825..10027)
                     /locus_tag="VBC81_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6911362.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            10183..10842
                     /locus_tag="VBC81_000297"
     CDS             10183..10842
                     /locus_tag="VBC81_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6911363.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            10866..13151
                     /locus_tag="VBC81_000298"
     CDS             10866..13151
                     /locus_tag="VBC81_000298"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6911364.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            13155..13514
                     /locus_tag="VBC81_000299"
     CDS             13155..13514
                     /locus_tag="VBC81_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911365.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            13560..14540
                     /locus_tag="VBC81_000300"
     CDS             13560..14540
                     /locus_tag="VBC81_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6911366.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(14625..16373)
                     /gene="cydC"
                     /locus_tag="VBC81_000301"
     CDS             complement(14625..16373)
                     /gene="cydC"
                     /locus_tag="VBC81_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6911367.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYNFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(16366..18084)
                     /gene="cydD"
                     /locus_tag="VBC81_000302"
     CDS             complement(16366..18084)
                     /gene="cydD"
                     /locus_tag="VBC81_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6911368.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(18084..19103)
                     /gene="cydB"
                     /locus_tag="VBC81_000303"
     CDS             complement(18084..19103)
                     /gene="cydB"
                     /locus_tag="VBC81_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6911369.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(19104..20531)
                     /locus_tag="VBC81_000304"
     CDS             complement(19104..20531)
                     /locus_tag="VBC81_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6911370.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(20634..21842)
                     /locus_tag="VBC81_000305"
     CDS             complement(20634..21842)
                     /locus_tag="VBC81_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6911371.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(21855..22754)
                     /locus_tag="VBC81_000306"
     CDS             complement(21855..22754)
                     /locus_tag="VBC81_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6911372.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(23163..23768)
                     /locus_tag="VBC81_000307"
     CDS             complement(23163..23768)
                     /locus_tag="VBC81_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911373.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGVFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            24651..24950
                     /locus_tag="VBC81_000308"
     CDS             24651..24950
                     /locus_tag="VBC81_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6911374.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            25110..26300
                     /locus_tag="VBC81_000309"
     CDS             25110..26300
                     /locus_tag="VBC81_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6911375.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(26370..27095)
                     /locus_tag="VBC81_000310"
                     /pseudo
     CDS             complement(26370..27095)
                     /locus_tag="VBC81_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(27222..27809)
                     /locus_tag="VBC81_000311"
     CDS             complement(27222..27809)
                     /locus_tag="VBC81_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6911376.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(27853..28389)
                     /locus_tag="VBC81_000312"
     CDS             complement(27853..28389)
                     /locus_tag="VBC81_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6911377.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            28524..29696
                     /locus_tag="VBC81_000313"
     CDS             28524..29696
                     /locus_tag="VBC81_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6911378.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(29740..30522)
                     /locus_tag="VBC81_000314"
     CDS             complement(29740..30522)
                     /locus_tag="VBC81_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6911379.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            30681..30950
                     /gene="rpsN"
                     /locus_tag="VBC81_000315"
     CDS             30681..30950
                     /gene="rpsN"
                     /locus_tag="VBC81_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6911380.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT        10469 a         6037 c         4990 g         9764 t
ORIGIN      
        1 ggaatttcag tgcagctagc caaaactgaa attaataaag ggaacacagg taatcttcct
       61 aaagaacata tcgaagcagt cttgagcgcc acaacaccat ttatgctatt tgtcactgct
      121 ttaatagtat tagtcaacat tgccattgtt attttctgta ttaaaaattt aagagcaatt
      181 aaaagaaatc aaatggttaa ttaccttcct tattaccttg gcttcgccat tactgtagga
      241 cttgttattt tgggttttct aactactaaa gcaccatggg caatagctat aaatatcgtc
      301 ttccaagcta ttttcggtct tctttatttc catgcttatc aaaaagcaca aaaactcaac
      361 gaacgtgatc ttgaagagac taactaaaag taaaagcact agaatctagt gcttttttct
      421 tttcaaagag agttaaatat agtcatcgtc ccaactttta aaaatctaaa tagatagctt
      481 catgttcatt ttccagggtc gttactgtaa cacctaagag tcgtatgcca aaaactgact
      541 cttctaaggt atcaaatatc gctttagcaa cttgatcaat gatttgaaaa tcttgagtat
      601 actcttctaa tgtcatacgt ttcgtcaatg tttcaaaatc cgcatatcta acttttaaaa
      661 cgattgtctt accaactttc ttatttttct ccagagaagc aacaactctc tgaacgttct
      721 ttgaaatctc agctttgata tctgcttcgt tatatagtaa tttcccatag gttttctcac
      781 ttcctattga tttacgaact ctattaggtt ttaccgggga agcattaatc cctcttgcct
      841 tacgataaag gtcatacccg aaacgaccaa acatatcaat taaagaaatt tcagataagg
      901 aaaggaggtc ctcaccagta taaaccccta aagcatgtaa tttttcgacg gagcgtttac
      961 caacaccatg aaatttctct attggtaagg gcttcaagaa atcttgagct tgatctggta
     1021 ggatgaaggt aagtcctttt ggtttctcaa agtcagaagc caattttgcc aaaaatttat
     1081 tgtaggaaat acccgctgaa caggtcaaat ggacatcatt ccaaatatcg tattgtatca
     1141 ttttagcaag tttaacagct gattttattc ccattttatt ttcagttaca tctaaataag
     1201 cttcgtctat actcatcggt tcaaccaaat ccgtatactt tttaaatata tctcgtactt
     1261 ccattcctac ttggcgatat ttttggtaat ttcccgaaat aaagatagct tgagggcatc
     1321 gttcatacgc ttcttttgaa gacatagctg aatgaactcc aaactttcga gcctcataat
     1381 tacaagtaga aacaacccct ctgccgccgg tttttctggg atcagaacca ataataacag
     1441 gctttccttt aagagaggga ttgtcacgct cttccacaga tgcaaaaaaa gcatccatat
     1501 caatgtggat aattttacgc gacgtatcat ttattaaagg aaaaattaac attgttctct
     1561 cccccattct ctctactcca ttataacaga ttacaactta atgatacaga ttaaaaaaac
     1621 tgtattataa aagaatcata attttgtcaa gaaatatgct tgcgaaaacg tttacttttt
     1681 cctaaaatgg atatgtaaac aaaattaaat ctgtcacaat gtttaaggag aaaactatgg
     1741 caacagttaa aacaaacaca gatatctttg aacaagcttg ggaaggcttt aaaggtgttg
     1801 attggaaaga aaaagcaagt attgctcgct tcgttcaagc aaactatgca ccttacgatg
     1861 gagatgaaag cttcttagct ggagcaactg aacgttcact tcacatcaaa aaagtgattg
     1921 aagaaacaaa agctcactat gaagaaactc gcttcccaat ggatactcgt gttgcttcaa
     1981 tttctgaatt accagctggt tttatcgaca aagataacga gcttatcttt ggtattcaaa
     2041 acgacgagct tttcaaattg aatttcatgc ctaaaggtgg tatccgtatg gcggaaacta
     2101 cacttaaaga aaatggttat gaacctgatc cagctgttca cgaaatcttc actaagtacg
     2161 ctacaacagt aaatgatggt atcttccgcg cttatacttc taacattcgt cgtgctcgcc
     2221 atgcacacac tgttactggt cttccagacg cttattcacg cggacgtatc attggtgtat
     2281 atgctcgtct agctgtttac ggtgctgact acttgatgca agaaaaagtt aatgactgga
     2341 atgcattgaa tgatattgat gaagagtcaa ttcgtcttcg tgaagaaatc aaccttcaat
     2401 accaagctct tggtgaagtt gtaaaacttg gtgaccttta cggcgtagat gttcgtaaac
     2461 cagccatgaa cactaaagaa gccattcaat gggtgaacat tgctttcatg gctgtatgtc
     2521 gtgttatcaa tggtgctgca acatcacttg gacgtgttcc tattgtcctt gatatctttg
     2581 cagaacgtga cctcgctcgc ggtacattca cagaatcaga aatccaagaa tttgttgatg
     2641 attttgtcct taaactccgt actgtaaaat ttgctcgtac taaagcttat gatgcccttt
     2701 actcaggtga cccaaccttt atcacaactt caatggcagg tatgggagct gatggacgtc
     2761 atcgtgttac caaaatggac tatcgtttct taaatacact tgacaatatc ggtaactcac
     2821 cagaaccaaa cttaactgtt ctttggtcag atcaattacc atatgctttc cgtcgctact
     2881 gtatgtcaat gagtcataaa cactcttcaa tccaatatga aggtgtatca acaatggcta
     2941 aagaaggcta cggtgaaatg tcatgtattt catgttgtgt atctccactt gatccagaaa
     3001 acgaagacaa acgtcacaac cttcaatatt ttggtgctcg tgttaacgtt atgaaagcac
     3061 ttcttactgg tcttaacggt ggttatgatg atgttcataa agattacaaa gtatttgaca
     3121 tcgacccaat tcgtgacgaa gtactcaact ttgacactgt aaaagctaac tttgaaaaat
     3181 ctcttgactg gttgactgat acttatgttg atgcgatgaa tatcatccac tacatgactg
     3241 ataaatacaa ctatgaagct gtacaaatgg cattcttacc ttcacatgta cgtgctaata
     3301 tgggatttgg tatctgtggt tttgcaaata ctgtcgattc attatctgcg attaaatacg
     3361 ctactgttaa accaatccgt gacgaagatg gttacatcta cgactacgaa acagttggtg
     3421 acttcccacg ttatggtgaa gatgatgatc gtgttgactc aatcgctgaa tggttacttg
     3481 aagccttcca tggacgcctt gctaaacaca aactttataa agatgctgaa gcaactgttt
     3541 ctcttcttac aatcacatca aacgttgctt actctaaaca aactggtaac tcaccggtac
     3601 ataaaggagt atatctcaat gaagatggta gtgtaaacct ttctaaagta gaattcttct
     3661 caccaggtgc taacccttct aacaaagcta aaggtggctg gttacaaaac ttgaattcac
     3721 tttctaaact tgatttcgct catgcaaatg atggtatttc attgacaact caagtttctc
     3781 cacgcgctct tggtaaaaca tttgatgaac aagttgataa cttggtaaca gtccttgatg
     3841 gttactttga aaacggtggt caacacgtta acttgaatgt catggacctt aaagatgtct
     3901 atgacaagat tatgaatggt gaagatgtta tcgttcgtat ctcaggttac tgtgtcaata
     3961 ctaaatatct aactcctgag caaaaaactg agttaacaca acgtgtcttc catgaagtac
     4021 tttcaatgga cgatgcatta actaactagt ttgtattcga tgaagtaggt ttgattatat
     4081 caaacctact tttatctata ttatcaatag tactaattac ttctttatat aacctcaagg
     4141 aggggcctaa tgttaaatca caaatttctt caagttctaa aatatgaggg tgtcgtttcg
     4201 ataacaagct ggaccgaatt agctcctcac gttacaaaca cttggaatag ctacctgact
     4261 attacagatg accagcgtat attagctcct gcagcaggaa tgactcatct tgaaatgatc
     4321 tcaataataa ttctaaaatt attatgactc tgggcagccg tgaagtcgag ggtcgtgatg
     4381 gttatcaagg tacaggcttt agaattgaag gaactgctaa gctattagaa gcaggaagcg
     4441 attttgaaat tgttaaagaa aaataccctt ttctgcgtaa agtattggaa gtaactccca
     4501 taaatgttat ccaactttta taaaaaactt ctcctagaaa attctaggag aagtttttta
     4561 ttcaaagtac gatgttatcg cctcttgtac atgtcgtcgt ttcttaatcc aaagtggacg
     4621 gtcatcatag gaaaaggttc tgtttgtcaa aaaaatagct gctgcctgct tttgataatt
     4681 tagtgcaata aaaggaccag tataaccagt atggtttatc caatccttat ctatatccca
     4741 agctaatgat cttgatttac tcaaactata attattccac aaaggcttcg caaaatcatc
     4801 ttttaaataa tgaatagaaa atctttgtaa atcatcaata gttgaaaata aacctgcaga
     4861 accagtatgc tttcctaata ccttagcttt aggatcgtgt acaataccat cgttaatacc
     4921 ggctacagtt ggaacagctt ctactctagg accaaaagag gtttctttca tctgaaaagg
     4981 agtgaaaata taacgtttga aaagtttatc caaactatcc cctaatacct cttctaacat
     5041 aaaaccaagt aaaagaaaat taatatcagt gtatttaaac gatttatctt ccaaaacctt
     5101 gatatgatta atggcatctt tcagctgagt agcattcaac tgatcacgat ttggaataaa
     5161 agggtcaatt ccactgctat gtgtagctaa ttgccttaca gttaacgtct gatgatgaaa
     5221 cgtagggtaa taatacctta atggcttatc aatatcaata gtcccttgat aaactaactt
     5281 tgctagtaaa gttcccacgc cgacaacttt tgatacactg gctagatcat agaccaatcc
     5341 cgctttaact ttctcattgc catcaattgt ccccaagtaa tgttcgtgcc actctccatt
     5401 ttcataaata gctaagcttg caccatgata aagattatct ttgatttgct ccttaatgag
     5461 ttctaaaacg gtattagttt taatcattct tttgaaatcc aaatctcaat attactagtg
     5521 tcagagatga ctaaaatctt ttctttagaa tcaaggtaaa catcaagtcc ccatgattca
     5581 aaagttgatt tcaagtcatt taaattagta tcttcattaa cacagcattc tagtatctca
     5641 atatcccaag tttcatttgg agcaatttgc aaatcttgtc cctgtgcttc tttgaaagat
     5701 aaattaattg ggaacttccc agcaaatact ttatcgtaaa aagcttcagc tttcacggca
     5761 tcaggtacat tcaaactaat agattggaaa gcaaagtttg atagacctgt agtgtcatct
     5821 tttttttcta actctgggcg ctcaattgcc actaattgtg acaaatcatc ttcagcatga
     5881 agtaagaaaa ggtctccctc gggcgaaact gtttcatagg cataaccatt ttgaccttga
     5941 tatacttgaa tggcttgcgc accatttgcc aaaagctttt caatatcttt tgcatcttga
     6001 gacttaacaa taatctttgc taacttctta gtaccattca ccgcacgagt acgataagtt
     6061 ggggattcct caataataaa gctagcctct ttgttctgcc atgctgagaa aactgcgatt
     6121 gcattttctt cacttatcaa tttaaatcca agagactcct gataaaatgc aatattcaag
     6181 tctctattat taacacgtaa tactgggtgt ttaaatgtca atgaatgaaa taacgtcata
     6241 aattcctcct tgttcagtct agtctatttt agataatttt agataattta gcaagaatct
     6301 ttgtgctctc aataccataa agtcaaattg acagtttcac tatttttttg tagactggat
     6361 attatgaatg aaacactact attacatggg attcagctta ttctgattat cgccatgatt
     6421 atcacttttt atcagattgt tagacatatt aggtctcaaa aaataaatcc tttcaaacga
     6481 tttttcactg gtttgtggat cggttttgta actgatgctc tcgatactct tggtatcggc
     6541 tcttttgcaa caacgaccac attttttaaa ctaactaagc ttgttgaaga cgatcgtaag
     6601 atacccgcaa caatgacagc tgcacacgta cttccagttt tgttgcagtc actctgtttt
     6661 atttttgttg taaaagtaga ggctttaaca ctaataacaa tggctggtgc agcttttatt
     6721 ggggcttttg ttggagctaa aatgactaaa aattggcacg ctcctactgt tcaacgtatt
     6781 ctaggaacac tgctcatcac agctgctatt attatgctct atcgtatgat cactaaccct
     6841 ggcgctggta tttcagattc agtgcatggt ctacacggca tttggttatt tgttggtatc
     6901 gtcttcaatt ttattattgg tgtattaatg actatgggac taggtaatta tgctcccgaa
     6961 cttatcttct tctccctaat gggactgagt cctgcagttg ctatgcctgt catgatgcta
     7021 gatgcagcta tgataatgac agcttctagt acacaattta tcaagagtgg tcgtgttaat
     7081 tggaacggct ttgccggtct ggttactggg ggaatattag gcgttattgt cgctgttctc
     7141 ttcttaacta accttgatct taacagcttg aaaacactgg ttgtaggtat tgttttattt
     7201 actggagcaa tgttaatacg ttcttccttt aaaaaaacta tgcccaaaag tcatcattaa
     7261 acgaaatcag ctagcaagac tggctgattt ttatatttaa aacaaaaaaa gaccggtaaa
     7321 ccgatctttc tttaactatt aaagatataa tagtttgaac aaggctacag cgagtatact
     7381 tgctacgatt ggtgctaaaa ctggcaccca tgcataccac cattttgaat cttctttagc
     7441 ttgtccaaga atttgttttg gtaataagcg gtgaacaata cgtggaccaa ggtcacgcgc
     7501 agggttcaat gcaggtccag tcgatccacc taaagaagca actagagtca ttaccaagaa
     7561 accaatacct aagtgagcta cagcaagtga accagttacg tatggtgaaa tccttgtcgc
     7621 tgctgtcgtt tggtcataac cagcttgaac aagtttacca acaagttcta caccaaaata
     7681 gttcttcgtt aaagccaaag ctccaaagaa aagaacaaaa gaaccaacga attcatttaa
     7741 gaaaccatta atgtaagccg ctttacgact tgatttctgt ccatcatcta atgcagaaat
     7801 agttgaaaat gatccaagaa catgatttgg attttctgtc ttaacaaagt aaggttgata
     7861 aaccataacg acaactaact gtccaaatat tgctcctaaa atttgtgcaa gaatgtattg
     7921 tccaacgtgt gcccaaggga aaagacctga aaatgccaat ccaagtgtga aagctgggtt
     7981 aatatggttt ccagagacat taccaaacat aagcgctggc atcataacac ctaaaccata
     8041 accaattgca ataataatcc aacctgaatt atttcctttt gtgcctttta agtcaacgtt
     8101 agctacagcc ccatttccta agatgataag aaaagcggtt gcgataaatt ctgtgatata
     8161 cttcacagtc caagtgattt cgatcaatcc ttgtttcctc cccaaaaaat atttacaaca
     8221 ttaaccattc taccaagtaa gcttaatatt gtaaagctta tcacaaaata aaattaataa
     8281 aatttttaac caaatatatt tgataaaaaa tagagtacta ggttcggact acctagtact
     8341 ctattttatt tagtttataa ggttttctta ctatcacgaa cgactagcat atcaactttc
     8401 gaatgacgta aaatgtattc tgaagttgag ccgatcaata ggctttcaaa agtgtttaat
     8461 cctgttgcgc ctaccataat taaatctgct ttttccctta tggggatatc ctttgccaaa
     8521 agagtcttag gatttccgat ttcagtaatt tttttaatct ttgtaaggcc ttttgaatgt
     8581 gctaatttct caaaataggc tagcacatct gctgactctt gttcttgcct atcaaaaaca
     8641 tagtcactaa agactccctc actttggtaa gcgtgagcat ctataacatg cgttagcagt
     8701 aattcagcat cattacgaag agcaacattt atcccttttt ctaccgccaa ttctgactcg
     8761 taagacccat caatagcaat aagaatacgc tcatattttt gtgtcatagc ttaattcctc
     8821 ccttttatat agttttagtt tcaacttgtc tagatgattt taagaaatat agaataccac
     8881 aaacaacagg aataatcgct gctatccaga ataattctct aaaggattgc acaccagctc
     8941 caagaaaacc atctttaaca agtcccaaaa tgtaaggtcc agcacctaac cctaaatcaa
     9001 gacctatcat gtaagttgac atggctgtat tgaaacgatg ttcctcaaca cctttgattg
     9061 atgctgcttg gccacaagac ataaaggtgc cataaccaaa accaatcaaa gcacctgata
     9121 gaaggtaggt aacacttccc atagcactcc ctaataaagc aagtcccaaa gttaagaaca
     9181 gataacttgg ataaagcacc cacttatctc ccttagcgtc cattaatctt cccatagatg
     9241 gtctggttaa agtgatgaca agtgcataaa caataaagaa ataagctcct actgtcatca
     9301 aattaatttc tgttgtatat aatttttgga aacctaacac ggaagcatag gagataccca
     9361 tcaaaaatgc aataattgtg ataaaaattg cttttttctc aatgaaacta tcaatagtcc
     9421 atgattttga tttagctaac tgttctggat ttaaagtaat atttttgact gggaaaacaa
     9481 atgctcccaa cactacaatc gcaattaaaa tactacataa tacaataacc attttaaagt
     9541 taatatgaag gttgtctagc ataaatgttc ctacaaaagg accaatagct gcggctaaac
     9601 ttgttgatag accgtaaaag ttaatcccct cacctctttt atcagctggt atataggctg
     9661 ttacaatagt attagttgct gttgacacga cgccataacc aaaaccattt aggaaacgaa
     9721 ctaaatacat tactccgata cttggcatat aaaaataagc taaagttgtt agtaagtaaa
     9781 aaatagcccc tccacgtaaa actaacttac gtcctagaac ttctaattgc ttaccaaata
     9841 taagacgagc aatcaaagtc cctacaatat aaatccccgt tgctaatcct gcttggctag
     9901 tgctaacacc taactcttta gtcgcaataa aagctatgat aacggtaaac aaataataga
     9961 ccatataaac aataaagttt aaaatagtaa tgcctataaa atgtttgttg aataatttgt
    10021 cttccaatgc aaatggctcc ttttttcaat ttcattttca taaacatttt acaaaaatat
    10081 tagaagatac gatatttcaa tggtataata taagtgctag tatcagactg cattaacaaa
    10141 tgttaaggtg gtctatcaaa cctaaaacat ttggaggaag tcatgaatca tcaaatttta
    10201 caaaattaca ttaatagtca taatcttcca attatcgaaa aagactacca taaatacctt
    10261 acatttgaaa gtttagaaga agactatact tatatcctaa aagatggtat tgttaaacaa
    10321 agtgttctct ctaaatatgg tactgagttc aatctgcgtt atgtgacagg cttagaaatc
    10381 acttctatcc taaatacaga ctactcccaa cacatgggag aaccttacaa cgttcgaatc
    10441 gaatccgaaa ctgcccactt ttacaaagta cgtcgctcta ccttcctaaa agatatcaat
    10501 aatgatattg aactgcaagg atatgtgaaa gatttctatc ataaccgcct tgaaaaatct
    10561 atgaaaaaaa tgcaatgtat gctcactaat ggtcgaattg gtgctatctc cacacaatta
    10621 tatgacctca gtaagatgtt tggtgaagaa cgagataacg gtgatatcta tattaatttt
    10681 gttatcacga acgaagaact tggtaaattt tgcggaatat ccacaggaag tagcgtgagc
    10741 cgcattttga aacaactaaa agacgatcat attattcgta ttgaaaaaca acatatcatt
    10801 attacaaatg ttgagaaatt aaaagatcat attgtatttt aatataacaa tagaaaggat
    10861 atattatgcg ttataatcaa ttcagctaca ttccaactaa acctaacgaa gcttttgaag
    10921 agctcaaagg actaggtttt ccattaaaca aaaagaattc tgataaagct aatttggaag
    10981 cttttctcag acattctttt ttaaatcaaa ctgatactga ttacgctctt agtctcctta
    11041 tcgttgatgc aaaaaccgat gctctaacct tttttaaatc aaatagtgac ttaacactag
    11101 aaaatttaca atggatttat ttacagttat taggctttat cccttttgta gactttaaag
    11161 accctaaagc attcttacaa gatattaact tcccagtctc atatgacaat atttttcaaa
    11221 gtctacatca cttactcgcc tgtcgtggaa aatctggcaa tacattaatc gaccaattag
    11281 ttgctgatgg tttacttcat gcagataatc actaccattt tttcaatggg aagtctctgg
    11341 ccactttcaa tactaaccaa ttgattcgcg aagttgtcta tgttgaaaca tccttagata
    11401 ctatgtctag tggtgaacat gatttagtaa aagttaacat tatcagaccc actaccgagc
    11461 atactatccc tacgatgatg acagctagcc cctatcatca aggtatcaat gatcctgccg
    11521 cagaccaaaa aacataccaa atggagggtg cgctagcagt taaacaacct aaacacatac
    11581 aagttgacac aaaaccattt aaagaagaag taaaacatcc ttcaaaatta cccatcagcc
    11641 ctgcaactga aagcttcaca cacattgaca gttatagtct caatgactat tttctttctc
    11701 ggggttttgc taatatatac gtttcaggtg tgggtactgc tggctctacg ggtttcatga
    11761 ccagtgggga ttaccaacaa atacaaagct ttaaagcagt cattgattgg ttaaatggta
    11821 aggttactgc attcacaagt cataaacgag ataaacaagt caaggctaat tggtcaaatg
    11881 gccttgtagc aaccacaggt aaatcttatc ttggtaccat gtcaactggt ttagcaacaa
    11941 ctggcgttga ggggctgaaa gtcattatcg ctgaagccgc aatctccaca tggtatgatt
    12001 attatcgaga aaatgggctt gtgtgtagtc caggcggcta ccccggtgaa gatttagacg
    12061 ttttaacaga attaacatac tcacgaaacc tcttagctgg tgattacatc aaaaacaacg
    12121 attgctatca agcattgtta aatgaacaat caaaagcaat tgaccgtcaa agtggggatt
    12181 acaaccaata ctggcatgac cgtaattacc taactcacgt caataatgtc aaaagtcgag
    12241 tagtttacac tcatggacta caggattgga atgttaagcc aagacatgtc tataaagttt
    12301 tcaatgcatt gcctcaaacc atcaaaaaac accttttttt acatcaaggt caacatgtgt
    12361 atatgcataa ttggcagtcg attgattttc gtgaaagcat gaatgcctta ctaagccaag
    12421 aactacttgg cattgacaat catttccaat tagaagaggt catttggcaa gataatacta
    12481 ctgagcaaac ttggcaagtt ttagatgctt tcggaggaaa ccatcaagag caaattggtt
    12541 taggtgatag taaaaaactt attgataacc attatgacaa agaagccttt gatacttatt
    12601 gtaaagactt caatgtgttc aaaaatgatc ttttcaaggg aaataataaa accaatcaaa
    12661 tcactattaa tcttcctcta aagaaaaatt atctcctgaa tggacagtgc aaactccatc
    12721 tacgtgttaa aactagtgac aaaaaggcca ttttatcagc ccaaatctta gactatggtc
    12781 ctaaaaaacg attcaaagat acaccaacca tcacattctt aaacagcctt gataatggta
    12841 aaaattttgc cagagaagct ttacgtgaac tcccgtttac taaagatcat tatcgtgtca
    12901 tcagtaaagg tgtcttgaac cttcaaaatc gtacagactt acttacaatt gaggctatcg
    12961 agccagaaca atggtttgat atcgagttta gcctccaacc aagtatatat caattgagta
    13021 aaggtgataa tctaaggatt atcctttata caactgattt tgaacatacc attcgagata
    13081 atgctagtta ctctataaca gtagatttaa gtcaatctta tttaactatc ccaactaatc
    13141 aaggaaatta acttatgaaa cttctcacta aagaacggtt tgatgattct caacactttt
    13201 ggtaccagat caatttatta caagagagta acttcggagc agtttttgac catgataata
    13261 aaaacattcc acaggttgtt gcaactattg ttgatgattt acaaggttcc ggaagttcga
    13321 attatttctg gtattttggc aatactactg atacttccat ccttatgatt gctcatttaa
    13381 atcgaaaatt ctatattcag gttaatttaa agaactttga ctttgcactc aatttaatag
    13441 ctataaataa ttggaagagt ctcctccaaa ctcaactcga agctctaaac gataccctag
    13501 caatatttca ataaataagg tagaatggag tgacaaagca acgcgaggga gactgattaa
    13561 tgtcatctta ttggaataac tatcctgaac ttaaaaaaaa tattgatgaa accaatcaac
    13621 taattcaaga aagaatacag gtcagaaata aagatattga agcggcgcta agccaactca
    13681 cagctgcggg aggaaaacag ctcagaccag cattctttta ccttttttct caacttggta
    13741 ataaggagaa tcaagatact cagcaactaa agaaaatcgc tgcttcttta gaaatccttc
    13801 acgttgctac attaatccat gatgatgtca ttgatgactc accactaaga cgtggaaata
    13861 tgaccattca aagcaagttt ggcaaagaca tcgcagttta cactggggat ttacttttca
    13921 cagtcttttt cgatcttatt ttagaatcta tggctgatac accatttatg aggattaatg
    13981 caaaatctat gcgtaaaatt ctcatgggag aattggacca gatgcacctt cgttacaatc
    14041 aacaacaagg tatccatcac tatttacgtg cgatttcagg taagacagcc gaactcttta
    14101 aattagctag caaagaagga gcttactttg gtggtgcaga gaaggaggtt gttcgtctag
    14161 caggccatat cggatttaac attggtatga cattccaaat tttggatgat atcctggatt
    14221 atactgcaga taaaaaaaca tttaacaagc ctgtcttaga ggatttagca caaggcattt
    14281 acagccttcc tctacttctt gccattgaag aaaatcctga tattttcaaa cctattttag
    14341 ataaaaaaac agatatggct actgaagaca tggaaaaaat tgcttatctc gtcgtttccc
    14401 atagaggtgt tgacaaagct cgccatctag ctcgtaaatt tactgaaaaa gctattagtg
    14461 acataaataa gctaccccag agctctgcaa aaaaacagtt gctacaatta actaattacc
    14521 ttttaaaacg caaaatttaa ataataaaaa aacattccac aatgctagaa aagcagttag
    14581 ggaatgtttt tttattatca tttatttatc gcacctatca atcatcatag atcaccatca
    14641 tcagcggctt tcagctgacg gtaacgttgg ctactttgag acaattcttg aggagaacct
    14701 tccaactcta attgcccatt ttctataaat aagatacgat cagcatgttc aatacctttt
    14761 aagtgatgtg taatccaaac taaggtctta ccttccaatt ctttcataaa tacccttagt
    14821 aaggcttgtt cagtaatagg atcaagtcca acagttggct catctaagat aacaattggg
    14881 acatctttta gtaagattct agccaaagca attctatgcc tttcgccacc tgaaaaccta
    14941 agtccagctt catcaaccat tgtatagaga ccatctgata aatcagtgac catctctttc
    15001 aatccaactc gttcaagaac tttccataca tcttcttcac tagcatcttg gtttccaatg
    15061 cgaatgttat ttagcagggt tgtattaaaa aggtagggcg cttgttgtat cactccaata
    15121 tagttagaaa tgcaatcacc aactattgaa acatcagcac cgcctagggt aatctcccct
    15181 tgacttgctt tcaagtcgcc acgaagtaga ctggctaagg tactcttgcc agaaccacta
    15241 cgccctaaaa tagcaatttt ttctccttct ttaatatcca aatctaaatg atgcaaaacc
    15301 catttctctt gtggcttata ctggaaactt aaattcttga cggaaaaatc gtatggctta
    15361 ttaggcaact gattttgagt tgtttcaaaa tacgtttctg ataattcatt aagacgatga
    15421 attgaatcag aatatttatt tgtttcttga gcagcagcag acaaaccagc aaatgcctct
    15481 gacaagggaa atactgttag aacaaatgct gcaatccagt tagcttcgcc acctctgtga
    15541 ccaataaact gattactagc ccaaataata accaaaatag ctagaaagcc aaaaactaat
    15601 tccacaataa gagctctacg attatcaaag gaacgaattt ttttctgaac agccatgagt
    15661 tcactttccg aacgctcatg caaggcgacg tactcttgac cacgttgact aaaaatccag
    15721 tctgaaatac ctaagacatt atccgtcaaa tcagtataaa gatgattttt taattctttt
    15781 tcacgagtct gacgagcacc attagctaaa atcgaccaaa gtggaaaaag aaaaatcata
    15841 atagctaaat aaagcaacat cattaaagca aaccatagag aaaagaagcc tgttgcaata
    15901 ataataaagt tatataatat ccacgctata atcgttggaa atattgtcct aagatacaaa
    15961 ttttgcaaat aattaatatc ctccgctaaa agccccataa catctcctaa acgaaaatca
    16021 cgcttcataa agatagcatt gctttctaag gaatggtaaa gcttcaagcg cagctgtgaa
    16081 gtcattcgta atacccaatt gtgactcgtt aggcgttcta tataacgaaa aacaggacgt
    16141 ccaatgccaa aagcacgtgt tagtacaatt ggaacatata ctagaagaat attactcggt
    16201 agtgaagctg acttactaat cagatagccc gaattaaaca taagagcact ggcagaaaag
    16261 aaagtcataa aaccaaggaa aagagcaatt actaaggaaa ctttatattg tttgaaaaat
    16321 ggttttatcc attgatctgt ctttaattct ttaaacaaag ggattttaaa catcatttcc
    16381 tcccatagca tgcttcagtt gatagagata tcctttttta gctaataatt cctgataaga
    16441 gcctacttct gcaactctcc ccttctccat aacgactata acatccattt ggttcaacca
    16501 gtgaagacga tgagttgcaa agataactaa acgatctgac atcagtggca acattttctc
    16561 ttttaattct aactctgttt caatatctaa atgagcagtt ggttcatcaa aaaccataat
    16621 acgacgattt tgatcaagaa aagcacgagc cagagctata cgctgggctt gtcctccact
    16681 aagcggacgc gctccattac caatacgcgt ttctaaaccg tcaggtaact cgcttagcaa
    16741 gctatctaaa cctaccatat gaatcgctct taccacctct tcgtcactag cattgggagt
    16801 atagaaggta atattatcac gtaatgacat ttcaaaaaca taaggagttt gaggaatata
    16861 gatcatctgt ttatgccaat cttcttgatc aaggttagtc acttctctac catccacttt
    16921 taaagaacca ttatcaggcc ctaaaaaacc actcagaagg ttaattaagc tagacttacc
    16981 tgaaccacta actcctacta aagcaacctt ttgatgtcct ctaaatcgta aggttacatc
    17041 ctcaacaaca cgtttttggt cgtacgcaat agcaatgttt tctaaatcca aacgactctc
    17101 tttagaccaa tcatctatga ctaattgctc acctttaata ccagttttgt ttagaacttt
    17161 ttgaatcgct tgaaatgcat ttttcccgtc taacgttgca tgataatcac tagaaaaatc
    17221 tcttacgggt aaaaagtatt caggggataa aatcaaaatt gttaaagccg gtaaaaggta
    17281 aatctgctca tttaataatc gtaaacctag aaaaacagca acgatagcaa tggataaagt
    17341 tgtaaaaaag tctaaagcaa acgttgataa aataccaatt ttcaaagtac tcattgttgc
    17401 tttacgaaaa gattcactag tttgataaat gcttttacca taacgtttac taagaccaaa
    17461 atatttgaga gtatcaatgc ctcgtaatga atctaaaaaa tgattagaaa gtacttgata
    17521 ggattcatat tgtttatctg ctttagcttg agccgcatag cctaaaataa tcataaagat
    17581 gataattaaa ggaaaaacga taagtaaaat tgcacctgat tcaatatcta aatagaaaat
    17641 ataagctaga ataatccatg ggataataga catgttaatc atcttattca agactaaacg
    17701 taaataattt tcaactaaag aaacgccatc taatgccatt gtaacaacat taccagttcc
    17761 ttgttcttga acgactttag gacctagatc aaataactta tctaataatt gacgtctcaa
    17821 aagactagat tgtgctgttg aaaagtcatc aagttttcta tttttgatgt aatcaataac
    17881 atgacggcct aaatatgaga ccataaataa caaaacataa accgtttgag aagaaagttt
    17941 ttgcccttcc cacaaaccag ttatagaaag actcaaataa taagcttgtc cgataataaa
    18001 gattgcttga agaacatcaa gtcccgcaag caatcctaac aacttatgaa tacctgacaa
    18061 gcgcataacc gctttatcta gcattatttt tctcctgtca caatcactgg caaggtaata
    18121 cgttttctaa agatataata tgcccatgct gtataagcta gaacaaaggg aactagagta
    18181 attgcgacaa tcgacattac ttttaaagta tatggcgttg aggatgcatt ttgaatcagc
    18241 aaatcatatt tagggctaat tgatgaaatc ataactcgtg ggaatagacc ttggaacaac
    18301 agaacaacca ctgaaacaag actgagacca ctcgctaaaa atgctgtcat ctctgctcct
    18361 ttaaaagttg aagcatgcgc taatacagct aatacaacaa taaccaaaac cataattgtc
    18421 gtaactatcg gatgattgct aaagaaatct gtcttgaaat acaagagaac tgcaaacaag
    18481 gctaaaccaa gatagagtac ccaataaagc aactgagcat aattacgggc acgctctcta
    18541 attggccctt ctgtcttcaa tgcaatgtaa tttaaaccat gaagataggc tagcaatacc
    18601 attgcaactc cacccaccaa cgagaatata ttaaagtaat cactgaattg agctgataag
    18661 ttaccactgg catcaagtgg cataccttga atcaaactta taaacataat cccaaagaag
    18721 aatggaacaa tagcggaacc aattgttaag gtccagttcc aaaactgctt tttctctgca
    18781 ggaactttat gtctaaattc gaaggaaaca ccacgaataa ttaatccaaa gagaatcgta
    18841 aggagaatca aatagtaacc actaaatagt gaagcatacc agtatggaaa agaagcaaac
    18901 attgctccac caccggtaag aagccaaact tcattaccat cccaaactgg gccaatggtt
    18961 tctactactt gatctttttc atgctcatta tgtgttaaag tttgaacagc catcccaacc
    19021 ccaaagtcga atccttcaag gaagaagaat cctgaaaata ggaggccgat taggaaaaac
    19081 caaaagaatt gtaaagcact catttagtat gctcctttct caaagggatc aattgaagta
    19141 tatgcaccat gatgtccgtg ttcatcttca tactctggcc ctttcttcaa ttctcttatt
    19201 gttaggctta tcatcatcac cgctaaacca ccaaacaaca agaaatacac agtattggaa
    19261 acaaataatg atgcaactga aacgtttgga gagacagagt ccttaatctt gaaaagacca
    19321 tagacagtcc aagggtatcg tccttgttct gtcacaatcc aaccaaatgt attggctaag
    19381 aatggtgcaa aggttgttaa agcaacaatc caaagcatcc atttgtgttt ataaagaatt
    19441 ggttttttct tacgagttaa aaagagtcct aaaacagaaa cacccataat agctgttccc
    19501 atcgccgcca ttgtacggaa accatagaaa agtaggttaa ccattggata gtagttatct
    19561 ttaccatact tagcaaccaa ttctttatta gctgtatcca tacctttcac agaaccagat
    19621 ggtttcccgt aagataaaat actcaacata taaggaatct taatcccaaa aacttgtttg
    19681 tgttctgctt catttgccca agcaactaca ctccaagctg caggatcacc cgaatcttca
    19741 taatcacctt ccatagcagc aaatttcatt ggttgatcgt gaatcaaggc tttcatttgt
    19801 aaatccccaa ctcccataac tgaaattgaa ccaagaagtg ctactactaa accaatacga
    19861 accgatttct tataaagttc aaccgttgta tctttcagct gttctgattt taagaggcgg
    19921 aaggctgaca taccagcaac cactgtacca cccatcgtaa tggcaccaaa aataacatgg
    19981 gtaaattcat agaagaactg tggatttttt acaagtgcaa gaaaatcagt catttgcgct
    20041 ctaccgttta caacttcata accaacagga tgttgcatga agctattagc agtaagaatc
    20101 cacatcgctg acattaatga gccaaaaacc actagccaaa tgaatgtcac atgtaatttt
    20161 ttactaattt ttttgttatc ccatgtaaac atccataaac caaggaaagt agattccata
    20221 aagaaagcta ataaagcttc aatagccaat ggagctccaa aaatatcacc tacgaaacgt
    20281 gaatagtctg accagttcat tccaaattgg aactcttgaa tgatacctgt cacaacacca
    20341 accgcaaaac taaggagcat gatattaccc caaaattttg tcaatttttt atactcttca
    20401 ttttttgtga taacatacat tgtctccata atagcaacca ctaggcatgt accaattgta
    20461 aatggtacaa agaaaaagtg gaaaactgta gtcatcgcaa attgaaaacg agctaaagtt
    20521 tcaattgtca tacaaaacct cttattttgt tttaatcatt tcttttatag aaatatctgc
    20581 aagagctagt ttcctagctt cgtaacaatt ggtaaataac ttactttaat atcttaatga
    20641 tataaatcaa aacgtccctt agctagaagt tctttaagtc cacccatatc aacaagtgat
    20701 ttgttcatga taatcttctt cataaccgac gcagggtatt ttttaatttt agtttttcca
    20761 acaacaccaa gtccatgcgt atttcctact gaagcaactg tgccttgcgg actatacgaa
    20821 aagtcttctg tagcttcacc tttgatttga tgtaaaaggt ttttagcaac atgagctccc
    20881 atacgagttg caatttgagc tgttgttggg aatgggcgac cagactcagt gtccataaat
    20941 gcagacacat caccgataac ataaacatta tcgtattttg gatcacgcaa atcactgttg
    21001 accattacac gtccacgacg ttgatcaaat cctgattctc ccataaccgg acttccacta
    21061 acacctgttg tccaaatgat agtacctgcg gtaatagatt tgagttcttc atcctcttta
    21121 ctagtaccat aaacaacttc acccggtttg atttccttaa tcatacttcc aagcatcaaa
    21181 ttaataccta aatccttaat taagttgaca ccatattgcg ctaattcatc atcaaacatt
    21241 ggtaggatgc gagttgctgc ctctacacaa ataatttcaa tttgttcagg ttttacaccc
    21301 gctaaagcag catattttgg tcgctcatca accatagctc cggctaactc aatacctgta
    21361 aaaccagcac cacatactaa gagtttgaga agattttcat cttttgtctc acggtacaac
    21421 ttcattgtat taacaatgtg attatggatg ttttctgctt gcgaaatatt ggtcatctgc
    21481 aaagcattct ccatagcacc cttgatgcca aaagtttcag atacaaagcc taaggcaact
    21541 acgacataat cataatggag ttcaccgtta tttttaactg ttactgtttt attttcagca
    21601 tcgactctaa gcacctcatc ttgcatgaag ttaaccttat tagtgttgat aacatcctta
    21661 attgggaatg taattttttc ttttggctga gagcctgcag ctacttcatg taattctgta
    21721 gcttcgtagt gataatcatt catgtcaaca agggtaatat gaaaatcacc tgattgtttt
    21781 tgtaaattac gaactgcttt caaaccggca taaccagcac ctaaaactag gatttctttc
    21841 atgataaaac tcctctaaaa aatgattcca attagatacg ttaagacttg ggttgcagat
    21901 cccagtgcta gaatacgtac cgcacaaaca aatgtttctc tcttaacctg tttttgccac
    21961 aatagttttg tctgcttata aataaatggt attaataaca aactcaagag tactgtaaca
    22021 ggcgcaagtc caaaaagaaa ttcaaaaaca attgccaata aagctatact attagaaata
    22081 gcaaacagca agagtccacc tcttatccct gtataatgta caagggtata gcgatgattt
    22141 ttttcatctt cttctttgtc gcacagatta ttcgccaaca ttaggttggc aatccataag
    22201 gtgttcggca aggaaatcaa gaaaattttg cttaagttga ggatatccca cgaaaagttt
    22261 tgataagtgt ttagatatac acaaattaaa ctaatcataa atcccattgt caagccagaa
    22321 aatacttctc ctaagggcag actagataaa ggtcttggac caaaggaata taaaacacca
    22381 attccaaaac aaaaaagtcc catccataaa agaggcagac cgacttggga aactaaaact
    22441 aaaccaatca tactagaagt aatgactaaa cttgccataa taacttctat ttggcgtagg
    22501 gataggttct cacgaccaat aatgttagtg tcattcttat aatccaaatc cacagcattc
    22561 cgataatcat tgtaattatt ccaaatgtca acgaacatat tgaataaaaa catagcaacg
    22621 aagaataaac cgacatatac agggtgtact gaattgtagt aataagcact aaaacacaaa
    22681 ccaatcaaaa atggtaaaac actagctgtt ttggctttca tttctaccaa ctctagaaag
    22741 attggtaatg tcattattaa agtcctctct atacataatc ttcaacaata ctattataat
    22801 gcaatttcta ccatttttaa aacaataggg cttgattttt gattaaaaat acaaaaaatt
    22861 tttcttaaaa tgagttaatc acacttagaa cactaaatac tattttgagg agtcctattc
    22921 atcacactat gccatgtaat attcggaaaa atagtattgc aaccctcaac tatcccttat
    22981 gttggcacca gcctttgaaa gtcctctgat tgcactgctt agttagtatt ttcatcagct
    23041 gtatgatctc taagagccat cagcatacta aaaaaactag tattacactt tgaaaagtgt
    23101 aacactagct ccaatttgtt ttaaatagct taactattta atcattaatc tactttatat
    23161 atttaagcat ttaaagttga atgttggttg aggatacctg agagagaacc ttgaatataa
    23221 tttggcaata cttgtttttg agcacgttca tagatttctg aatctgttaa ctgacgttct
    23281 gatttggggc ttgatacttg cggtgtcact gctgcatttt gttgtttgta aagtgttgaa
    23341 tgctggttaa ggatacctga gagagaacct tggatatatt ttggtaatac ttgtttttgg
    23401 gcactttcat aaagttctga atctgttaac tgacgttctg atttggggct tgatacttgc
    23461 ggtgtcactg ctgcattttg ttgtttgtaa agtgttgaat gctggttaag gatacctgag
    23521 agagaacctt ggatatatct tggcaatact tgtttttggg cacgttcata aagttctgaa
    23581 tctgttaact gacgttctgc ttttggaggt gttgcttgcg gtgctgtgaa agttgaagct
    23641 gaaacacctt gtgtagaacc aagagctacg aatactcctc cagcaaacat tgctgagaca
    23701 agaccaagtt tcaaatagtt agcagttgag acgttgttga cttttttttc tgtaataagt
    23761 ttgttcataa gaattacctc tttcttttgg gttatttatt ttatgaacta agtataacaa
    23821 caagaaataa agtaaggtta aactaaatat aaaataattc ttaagtcatt cttaggaaac
    23881 cataaaatca tacactaaca aaaaatttgg tatctagctc aaaagtaaaa gtagaataaa
    23941 ggcttttact tctgagctag taatgaataa attatatttg catctagttt ggaattagcc
    24001 ctagtgtatt ataatgatcc ctacaaatat gtgccaaaca ccttcttcat atatacaaga
    24061 ttgatttaac attcctatac ccttaatggt gggcacattc tcttcatgtt ccatatagaa
    24121 atcaacagat aattccgtat tgtccctatt attaaatttt ccagttaatg ataaattata
    24181 tcatccacct tgaggtgtcc tattaccaat ctgttcaacc tttacacttg acgaatcgaa
    24241 ttgaacagat tctgtcttat cattctgtga tttagaacat caacaatttc ttgttcatgt
    24301 tctttgaggt aagctagttg ttcttcttta acgtgctttt atctttcgta cttactgata
    24361 cttgagagca tttttttgac cctacttctt actaatcagc tgacgtccct ataagatgaa
    24421 tcataaacac atagctgcca ctaaccattt taacctttta tgcatacata attcttcttg
    24481 atatcccata ccattatctt tagtataaac acttaataaa acaagcattt aacaactaag
    24541 gttaataaaa ttcaataaat ctaatccttc tacttacttt agtttatcac ttttttctct
    24601 catcctatgc tataatagta aggtatcaaa atttaggaga atatagtctt atgatgaata
    24661 tgcaaaatat gatgcgtcaa gcacaaaagc ttcaaaaaca aatggaacaa acgcaagctg
    24721 atttagcagc tagccagttt actggaaaat ctgctcaaga gctcgttacc gtgacattta
    24781 caggcgataa aaagctaatt tcaattgatt acaaagaagc tgtggtagac cctgaagata
    24841 ttgaaacact acaagacatg acaactcaag ctatcaatga cgctttaagc caagttgacg
    24901 atgctacaaa aaaaattatg ggagcatttg cgggtaaaat gccattctaa aaaataaatc
    24961 acatttactg aatcacctgt aaatgtgatt tttatttcaa acttctttca ttttttcacg
    25021 aaaacaagtt ttattttcaa aaaatatttg caaaaaatca gcagagaggt ataataacat
    25081 tgaaaatatt agagagaagg tgactaacca tggttatgga tagtctaatc ataaaacagt
    25141 tgattaagag tacatttgat atacccttac aagtcactta tccaaatggg aatattgaaa
    25201 cttataatgg tagcaatccc cacgtcaaac ttaaactaaa caaaaatttc tccgtctcgg
    25261 agctatcaaa agacccttcc atagtattgg gtgaagctgt tatggatgga gacattgaaa
    25321 tttatggttc tatacaagaa ttaattttat cagcttaccg ctgtggagac agctttctac
    25381 gtaatagtaa atttagcaaa ttgattccta aacaatttca tgataaaaaa cacagtaaat
    25441 ccgacattca aaagcattat gacatcggaa atgatttcta taaactgtgg cttgatgata
    25501 ccatgactta ttcatgcgcc tattttaaac atgaaaatga tagtctagag caagctcaac
    25561 taaacaaagt acaccatatt cttaataaat taaatgctca gccgggaggg aaattactag
    25621 acatcggttg tggctggggg acacttatca ttactgctgc taaagaatat ggcttaaatg
    25681 cgacaggtat caccttaagt gaagagcagg cttctttcat tactaagcgt atcaaggaag
    25741 aagggttgga aaataaagta acagtcctca ttaaagatta tcgtgacatt cgtgaaacat
    25801 acgattatat cactagtgtt ggtatgtttg aacatgtagg aaaagaaaat ttatctcaat
    25861 acttccaaac catttctaaa agactcaaca tcaatggtct agctcttatt catggtatta
    25921 ctggacaagt gggtggtaat catggtagtg gaactaattc ttggattaat aagtatatct
    25981 tcccaggagg atatatccct cgtttaactg aaaatttaaa tcatatagct agtgctggac
    26041 ttcaaattgc tgacttagaa ccgcttcgtc gtcactatca aaagactctc gaattatgga
    26101 caaaaaattt ccacaatgcc cttcctgaag ttcagaaaac tcatgataag cgttttatca
    26161 atatgtggga cctctatctt caatcttgtg cggcctcttt tgaatctggc aatattgata
    26221 tttttcaata cttgctttca aaaggtgttt caaaagacac tatgcctatg acacgcgatt
    26281 atatgtattc tgctaattga tatagttaat attatagtat tccaacttat tctaaataaa
    26341 aaggactctc ttgaattcaa gggagttctt taacgttctt ctctggctaa ctctatacaa
    26401 tgtgactttt ggtgacaatg tggacagaat acttttcgcg tcttaggggt atgtttcgct
    26461 agattaaatg cttgatatgt cggtgtaaag gtttgatgac aatttggaca aagataaaca
    26521 acctgttgtt taaaatacca aaccatacca ttcatagcta ttaagaatac tgttactgct
    26581 atccacataa gccatctttg atttagagaa actgctgcta ttattaacgt aatatagacc
    26641 ataataatca taacgacttg tagcactatg cttttttgta gtatacccca ttttcttttg
    26701 ttttcttcca taagcacaat gtcagaaaga tagcctaatg ttttactatc ttgtttttct
    26761 acagttttta gtaggttgac agctttgtca agtttgtctt ttttactatc aatagctagc
    26821 cttaattctc ttatctacac ttgtaaaaaa agctctaaaa tctggctagc attgtcttca
    26881 gtaaatagat ttttgatatt atcaattgaa aaatctaaat ctcgtaaata aataatttgt
    26941 ctaagctttt ctaaatcagc aatactataa accctcctac ctccttcaga tagttctgat
    27001 ggggataaaa ttcctctttt gtcatagtac tgaacagtac gaacagtaac tcctgcttct
    27061 ttagctaagt caccagtgga atatttttct gacatgtttc acacctcctt taatatagta
    27121 aacacttctt tatcagaaac acctttacta tatcaaatga cctaaggtaa taagcaagta
    27181 ataacgctaa gtatttttaa ctttaaataa aactacaaca attaatcaaa caaatttcct
    27241 aaagtcgcaa atgggctatc aactgctacc tctttttgct gttttaaata gattttattt
    27301 tcatccaagt ccagaaattt ttcataaaca cgcgcagtca tacgggcatc ctctaagcta
    27361 ttatgtgctt taccctttat tcctaaaaag tcagcaacag tagtcaattt aaggttgaca
    27421 ataccattta aatctgtact gcgtctcaca aaagcttcat catataagtc cacctgatat
    27481 tgactagtta gatccaaacc attttcaact aaaaggggta aatccgattt atacccatta
    27541 taaccaatca gtggtgtatc tccaacaaaa ctctgaaagt ctgtcaaaac tatctcaatt
    27601 ttaggagccc ctataatatc tctagctgta atacctgtta aaccattaat aaagctttgt
    27661 aatggaactt ttgtgtgaac ataagtatca aataaagcaa tctcttgatg attagaatat
    27721 ttaacggccg acacttgaat aatatggcta tgctcaccaa ccgtgttaaa ctctaaatca
    27781 aaagctatat attcttgtaa ttgtttcatt atttctccta aaaagcgcaa ggaaactcct
    27841 tacgctttta ttctattttt tattgaagaa acgtccaaag aaactttttt taacagcttc
    27901 taattcttga attttttctt ctttggcttc ttgaatctcc tcaacctctg ctttagcttc
    27961 atttaactca agttgcagac gttgcgtatc ttccatagca gttaaagtca attgctgttg
    28021 ttgatctaat tgcttatctt tctctgctat ttgaatatct tttacatgta actgctcgtc
    28081 ttttgaagca atctgaatat cttttgcttt taactgttca tataaacgtg tgatttcagt
    28141 attcttctca tcaacgagaa tttctaaaag ttctcgtctt gatgcttctt cgtcaattgg
    28201 ttcctcttcc aaaatagttt ttttatagat ttcttccaat ttaatcaagc catcacgatt
    28261 aacaactgta acgcctttac tattcttttc cacacattct tcagggagtg ttttaactct
    28321 gttattcatc gcttgacgac taacccctaa aatttcagct aattcactaa ccgtcttttc
    28381 aactgtcata acttcctctt ttgtactgat tttcttataa aagaatatca caatcctaat
    28441 ctagtgtcaa tttactgtca acttaaagtg tcaaacaaga cattttgacg tctatttctc
    28501 tatttatatg ctagaataag attatgaagc attatgacac aataattata ggtggtggac
    28561 ctgctggaat gatggcagct atttcctcaa atttttacgg caacaaaaca cttcttattg
    28621 agaaaaataa acgtttaggc aaaaaactag ctggaacagg tggtggccgc tgtaatgtta
    28681 ccaataatgg aaaccttgat gagctcttag ctggtatacc tggaaatggc cgtttcctat
    28741 atagcgtctt ctcacaattt gataaccatg atattattaa cttctttcaa gacaacggcg
    28801 tcacacttaa agaagaagat catgggcgta tgttccctac tactgataaa tctcgtacta
    28861 tcattaacgc ccttgaaaat aaaataaaag aacttggtgg tcaaattatg actgacacag
    28921 aagttgtctc tgtcaaaaag ataggagatt cattctatat caaaactaaa gatacacagt
    28981 tcgcatccga taaacttatc gtaacaactg gtggtaaatc ctatccgtct acaggttcta
    29041 ctggtttcgg tcatgacatt gctcgtcact ttaaattaga agtcacagat atggaagccg
    29101 ccgaaagccc tcttttaact gattttcccc ataaaaaact acaaggtatc tccttagatg
    29161 atgtcacttt gtcatttgaa aagcatatta ttacacacga tcttctcttt actcattttg
    29221 gattatcagg accagcagcc ttgcgaattt ctagctttgt caaaggcgga gaaactatat
    29281 acctagatgt tttgcccaat atctctgtta aagaattgga aatacacttc caaaatgaaa
    29341 gagaaaaatc tttgaaaaac gctcttaaaa tacttcttcc tgagcgttta gctgaatttt
    29401 acgctgaaga cttacccgaa aaagtaaaac aagtatctgt aaaagactta gaaatgttaa
    29461 ttcaaaaact taaaaaactt cctattttag taactggcaa gatgtctcta gcaaaatcct
    29521 ttgtaacaaa agggggagtt gatcttaaag aaataaaccc caaaacactt gaaagtaaaa
    29581 aggtagctgg tttacatttt gctggtgaag tcctagacat caatgctcat acgggagggt
    29641 ttaacattac ttctgcctta tgtacaggct gggtagctgg tagcttacac tattaataca
    29701 tgaaagctga acaattccgt cagctttttt atatagaatt taaagataat atttggctct
    29761 taaggcttct ttgatttttt ccaattcatc tggtaaattt acctgatgaa tagcataccc
    29821 aatctgataa ggggatacta taaaacgagg aacaatttta caaataagac ccccatcatg
    29881 cttatagaaa aagagattat aagatttaca gcgccctccc aagaattcat ttaaaatata
    29941 atgacacaaa attggtaaac tatctgcaat caaaatagcg ttttccaatt tggatacttt
    30001 aattgtgaac tctgtgaaat caagcatagg acgattggca atactaatct ctgcttgttg
    30061 actcgtttcc gaacaaacct cgataccatc aaaccaatct aagttcaacg tttcataagc
    30121 atctacgttt tttaagccaa caatttggct atgaggatgt cgtaaacttc cgccagataa
    30181 gggcccaaaa tttcgataaa gaacgaccga acggtaatga ccactttctt ccattaattg
    30241 ccattttgac aaaagataat ctaacaattg gcgcatcttt gactcactat aaactgaaat
    30301 atccccattg tgatcatttg attcaatcac caatgtttga taggtttctt ctaaagttgg
    30361 gtatttattt tctacccaga tcatatcgtc tttcacgtct aggatttttc ctaattggct
    30421 tttatcacaa aatggacatt tgtctttatt gctgggtttt tgtttcgcga tattagggtt
    30481 aaatcggata tatctttcat tcatactgtt attgtaacac attcatcacc attacaatat
    30541 aatacttatc cagttaacat tttccttgca ttcttagctg aaaagagtat aatagaatca
    30601 tgtcttgttt accggttaat taccagttaa ctaggcaccc tgttttggga tagaaaaatc
    30661 tttttgggaa aggagaaatc atggctaaga aatcaaaaat tgctaaattt caaaaacagc
    30721 aaaaactcgt agaacaatat gctgaactac gtcgtgagtt aaaagaaaag ggtgattacg
    30781 aagcacttcg taaacttcct aaagattcta accctaaccg acttaaaaat cgtgatttaa
    30841 tcgatggccg tcctcacgct tacatgcgta aattcggtat gagtcgtatt aacttccgta
    30901 acctagctta caaaggccaa attcctggca ttaaaaaagc aagctggtaa ttcgtgaaga
    30961 caaatagtta ctttttatga ctattctatt gagtgtggac gaatagttaa tacattgtaa
    31021 ttatttaacc tgttgagtat ttactcagct attacaaact tcttcaaaat tttattatca
    31081 taggaatgta ggatctcatc aatccttaaa tgactaataa taaaagatga aaaaattttt
    31141 cttatctcaa atcatagatg ccaatttatt tattttcaaa aagcccggta tgccaagata
    31201 ccgagctttt tattattata aatactgata aaattctttt agttaatccc aaaaacaaga
//
LOCUS       DATRNX010000008        30763 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRNX010000008 DATRNX010000000
VERSION     DATRNX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30763
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            15..1205
                     /locus_tag="VBC81_000316"
     CDS             15..1205
                     /locus_tag="VBC81_000316"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6911381.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            1224..2612
                     /gene="argH"
                     /locus_tag="VBC81_000317"
     CDS             1224..2612
                     /gene="argH"
                     /locus_tag="VBC81_000317"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6911382.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            2752..3633
                     /locus_tag="VBC81_000318"
     CDS             2752..3633
                     /locus_tag="VBC81_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6911383.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(3718..4635)
                     /locus_tag="VBC81_000319"
     CDS             complement(3718..4635)
                     /locus_tag="VBC81_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6911384.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            4874..5062
                     /locus_tag="VBC81_000320"
     CDS             4874..5062
                     /locus_tag="VBC81_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6911385.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            5194..5559
                     /locus_tag="VBC81_000321"
     CDS             5194..5559
                     /locus_tag="VBC81_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6911386.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            5559..7223
                     /locus_tag="VBC81_000322"
     CDS             5559..7223
                     /locus_tag="VBC81_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6911387.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            7371..8255
                     /locus_tag="VBC81_000323"
     CDS             7371..8255
                     /locus_tag="VBC81_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6911388.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(8347..8463)
                     /locus_tag="VBC81_000324"
     CDS             complement(8347..8463)
                     /locus_tag="VBC81_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6911389.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(8831..9061)
                     /locus_tag="VBC81_000325"
     CDS             complement(8831..9061)
                     /locus_tag="VBC81_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911390.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
     gene            complement(9403..10143)
                     /locus_tag="VBC81_000326"
     CDS             complement(9403..10143)
                     /locus_tag="VBC81_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6911391.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            complement(10153..11703)
                     /locus_tag="VBC81_000327"
     CDS             complement(10153..11703)
                     /locus_tag="VBC81_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6911392.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            11839..13722
                     /locus_tag="VBC81_000328"
     CDS             11839..13722
                     /locus_tag="VBC81_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6911393.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            13768..14607
                     /locus_tag="VBC81_000329"
     CDS             13768..14607
                     /locus_tag="VBC81_000329"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6911394.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            14727..15482
                     /gene="mecA"
                     /locus_tag="VBC81_000330"
     CDS             14727..15482
                     /gene="mecA"
                     /locus_tag="VBC81_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6911395.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            15484..16644
                     /locus_tag="VBC81_000331"
     CDS             15484..16644
                     /locus_tag="VBC81_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6911396.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            16809..17579
                     /gene="sufC"
                     /locus_tag="VBC81_000332"
     CDS             16809..17579
                     /gene="sufC"
                     /locus_tag="VBC81_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6911397.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            17616..18878
                     /gene="sufD"
                     /locus_tag="VBC81_000333"
     CDS             17616..18878
                     /gene="sufD"
                     /locus_tag="VBC81_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6911398.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            18880..20112
                     /locus_tag="VBC81_000334"
     CDS             18880..20112
                     /locus_tag="VBC81_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6911399.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            20099..20542
                     /locus_tag="VBC81_000335"
     CDS             20099..20542
                     /locus_tag="VBC81_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6911400.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            20642..22060
                     /gene="sufB"
                     /locus_tag="VBC81_000336"
     CDS             20642..22060
                     /gene="sufB"
                     /locus_tag="VBC81_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6911401.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(22131..23318)
                     /locus_tag="VBC81_000337"
     CDS             complement(22131..23318)
                     /locus_tag="VBC81_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6911402.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKLGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(23471..24706)
                     /locus_tag="VBC81_000338"
     CDS             complement(23471..24706)
                     /locus_tag="VBC81_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6911403.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            24948..26606
                     /locus_tag="VBC81_000339"
     CDS             24948..26606
                     /locus_tag="VBC81_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6911404.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            26721..27635
                     /locus_tag="VBC81_000340"
     CDS             26721..27635
                     /locus_tag="VBC81_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911405.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            27645..28676
                     /locus_tag="VBC81_000341"
     CDS             27645..28676
                     /locus_tag="VBC81_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911406.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            28689..29738
                     /locus_tag="VBC81_000342"
     CDS             28689..29738
                     /locus_tag="VBC81_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911407.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            29735..30667
                     /locus_tag="VBC81_000343"
     CDS             29735..30667
                     /locus_tag="VBC81_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6911408.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         9962 a         4955 c         6082 g         9764 t
ORIGIN      
        1 gaaggggatt ttttatggga aaagaaaaat taattttagc ttattctggt ggtttagata
       61 cctctgttgc tatagcttgg ttaaaaaaag attatgacgt tattgctgtc tgtatggatg
      121 ttggcgaagg taaagatctt gattttattc acgataaagc tttaacaata ggtgccatag
      181 aatcttatat tttagatgtt aaagatgagt tcgctgagca ttttgtttta ccagctttac
      241 aggcacatgc gatgtatgaa caaaaatacc cgcttgtttc agcgcttagt cgtccaatta
      301 ttgcccaaaa attagttgaa atggcacatc aaacaggtgc gaccacaatt gcacatggat
      361 gtacaggtaa aggaaatgac caagtgcgtt ttgaagttgc aattgcagca ctagatcctg
      421 aattaaaagt aattgcccct gttcgtgagt ggcagtggca tcgtgaagag gaaattactt
      481 ttgcaaaagc taacggcgta cctattccag cagatttaga taacccttat tctatagacc
      541 aaaatttatg gggacgtgcc aatgaatgtg gtgtgcttga gaatccttgg aatcaagcgc
      601 cagaagaagc ttttggtatt acaaaatcac cagaagaagc tcctgattgt gcagaatata
      661 ttgatattac gttccaaaat gggaagccta tagctataaa caatcaagaa atgacactag
      721 cagatttgat tttatcgcta aatgaaattg caggaaaaca tggtattgga cgcattgatc
      781 atgttgaaaa tcgtttagtc ggtatcaaat cacgtgaaat ttatgaatgt cctgcggcaa
      841 tggtactgtt agctgctcat aaagaaattg aagatttaac attagtccgt gaagtgtctc
      901 attttaaacc tatccttgaa aatgaactat caaatcttat ttataatgct ttatggttta
      961 gtccagctac aaaagctatc attgcctatg tcaaagaaac acaaaaagtg gttaatggta
     1021 ctacaaaagt taagttatat aaaggttctg ccaaagtcgt tgctcgccac tcgtctaatt
     1081 cattatatga tgagaactta gcaacttata cagctgctga taactttgat caagatgcag
     1141 cagttggttt tatcaaactc tggggacttc caacccaagt taatgcacaa gtaaacaaag
     1201 gataaataaa ggaagataag aggatgacaa aaaatcataa gttatggggt ggtcgttttg
     1261 aaagtagtct tgaaaaatgg gttgaagagt tcggtgcatc aatcagtttc gaccaaaagt
     1321 tagctcccta tgatatgaag gcttctatgg cccatgtcac aatgttaggc aaaacggata
     1381 ttattagtca agaagaagca ggcttgataa aagatggttt aaaaatactg caagataaat
     1441 acagagcagg tcaattgact tttagcatat caaatgaaga cattcatatg aacatagaga
     1501 gcttattaac cgctgaaatt ggagaagtag caggtaagct tcatactgca cgttcacgaa
     1561 atgatcaagt ggctacagat atgcaccttt atttgaaaga taaattgcaa gaaatgatga
     1621 agaaattgct tcatttacga acaaccttag ttaatttagc agaaaatcat atctatactg
     1681 ttatgccagg atatacacat ttacaacatg ctcagccgat ttcatttgga catcatctaa
     1741 tggcttacta taatatgttt acgcgtgata cagagcgatt agagtttaat atgaaacaca
     1801 ccaatctatc accattagga gcagcagctc tagcagggac aacatttcct attgaccgcc
     1861 atatgacaac tcgtttatta gattttgaaa aaccatatag taattcgctt gatgctgtct
     1921 cagatcgtga ctttatcata gaatttttat ccaatgctag cattttaatg atgcatttat
     1981 cacgtttttg tgaagaaatc attaattggt gcagttatga atatcaattc atcacattat
     2041 cagatacttt ctctacagga tcatctatta tgcctcaaaa gaaaaatcct gatatggcag
     2101 aattaattcg agggaagaca ggccgtgttt atggaaattt atttagttta ctaactgtta
     2161 tgaaatcatt gccgttagct tataacaaag atttacaaga agataaagaa gggatgtttg
     2221 atagcgtaga aacagtcagc atagctattg aaatcatggc taatatgctt gagaaaatga
     2281 ctgtgaatga gcacataatg atgacctcaa cggagactga tttttcaaat gcaacagaat
     2341 tagcagatta tttagcaagt aagggggtac ctttcagaaa agcacatgaa attgtaggta
     2401 aattggtttt agaatgcagt aaaaatggtt cttatttaca ggatatacct ttaaaatatt
     2461 atcaagaaat ttcagagttg atagaaaacg atatctatga gatattaact gcaaagacag
     2521 cagttaaacg aagaaattca ttaggaggaa cagggtttga ccaggttaaa gaacagatcc
     2581 tgttggcacg taaagaattg aaagcggaat gactgctttc tgaaaaaacc tcgtttaact
     2641 ctgttaagtt tctcacgtat tttcagtaaa aaactagcat tttttataga attgtgctag
     2701 aattaacatg taagtgggca acgcccaaaa aataaatagg aggcctgaat aatggcaatc
     2761 gtttcagcag aaaaattcgt ccaagcagct cgtgataacg gctatgcagt aggtggattt
     2821 aacacaaaca accttgagtg gactcaagct atcttgcgcg cagcagaagc taaaaaagct
     2881 ccagtactta tccaaacttc aatgggtgcg gctaaataca tgggtggtta caaactttgt
     2941 aaacaactta ttgaaacttt ggttgaatca atgggtatta ctgtaccagt tgctatccac
     3001 cttgaccacg gtcattacga tgatgccctt gagtgtattg aagtaggtta cacttcaatt
     3061 atgtttgatg gttcacacct tccagttgaa gaaaaccttg aaaaagcacg cgaagttgtt
     3121 gctaaagcac atgctaaagg tatttcagta gaagctgaag ttggtactat cggtggtgaa
     3181 gaagatggta ttgtcggtaa aggtgaatta gcaccaatcg aagacgctaa agctatggtt
     3241 gaaactggta ttgacttctt ggctgcaggt atcggtaaca ttcatggacc atacccagca
     3301 aactgggaag gacttgacct tgatcacttg aagaaattga ctgaagctgt tcctggtttc
     3361 ccaatcgtgt tacacggtgg ttcaggtatt cctgatgatc aaatccaaga agctatcaaa
     3421 cttggtgttg ctaaagttaa cgttaacaca gaatgtcaat tagctttctg tcaagcaact
     3481 cgtgactacg ctgttgaatt caacgctaac gaagctgaat acatgaagaa aaaactcttt
     3541 gacccacgta aattcttgaa acctggtttc gatgcaatta cagaagcagt tgaagaacgt
     3601 attgacgtgt ttggttcagc aaacaaagca taattaacat aaaacagata atagttaaaa
     3661 gctcacttat ggtgagcttt ttttggatta tatgactttt tattaaatag atactcttta
     3721 aagattgata tgcttaagat aatttcgaat aaatgccgct gaatctgtga gttgttgctc
     3781 ttcttttggg cttagtgaaa gagaaattct ttctacaatt cctactctac caataatagc
     3841 tggatatcca aggtaaaccc cttcggactc atagaaggta gatacaggta gtaactcatg
     3901 ggaatcattt aacacagcta aaacgatgcg gacagctgcg gtagcgatac cataactagt
     3961 atatttttta ccaaaaaaga cagtgtgccc gccttttaaa gaagcttctt ttatctgttc
     4021 taattcttct acttttaata gtgatatagc tggttgtcct tttacagtta cctgactcca
     4081 agcgacaaat tgggagttac catgctctcc tagattataa ccaccgatac tccttggatc
     4141 aacttgtagg cgttgaccaa gcactctttt catccgagca gtatccaata aggttcctgt
     4201 tccgataacg cgtgatttag gaaattgagt atattcttga taaagctggg tgatggcatc
     4261 aacaggatta gtaacgacaa taaggatccc tctaaaacca gatgatttta acttttggct
     4321 aacgtcgtag acttctttac tggtaaatgg tagttcagca aagcgattag cttctttgtt
     4381 attctgtagt gaaatatcac ctaaggctga aatcacaaca tcagcatctg acaacgcgtc
     4441 atagtcgttt aatactatat tagcgtgata agtagcattt gccagagcat cttgaaaatc
     4501 aatcatatca gcattaacct tatcaacatt agtatcaata aagacataat catcaccaat
     4561 accttgtgca ataagagcgt gtgcaacagt tgatccaacg tgccccatgc ctataattcc
     4621 aattttacgt gtcatatgaa acctcctttg attattgttg tctattataa tattttgaaa
     4681 gaaaatgtaa actaaaaaat gaattagaag gaagtgtgtc ttattataaa aaatatgtaa
     4741 gcgatttaga taaaacaaat gaaaaagtaa ttttgaaaag aacttgctag aagaagtaag
     4801 ttgtgataga atagtctagt atgtggtgct agcacatccg taaatattga tcaatatagg
     4861 aggaactcgt aaaatggcta aagtatgtta tttcacagga cgtaaaacag tttctggtaa
     4921 caaccgttca cacgcgatga accaaacaaa acgtactgtt aaaccaaacc ttcaaaaagt
     4981 tactgttctt atcgacggta aaccaaaaaa agtttgggtt tcagctcgtg cgcttaaatc
     5041 tggtaaagta gaacgcgttt aataaactga gaaagcctta gggctttttt tagtgtttct
     5101 aaggctgatt tgatagttag atacctctat ttttttacaa taatagtgag atatgataga
     5161 atagagtgat aaaaactttt tgaggtagta gatatgactg taaaaatcaa tacaagagat
     5221 ggtcaaattg aactctctga tgacgttatt gcaactgtcg ttggaggttc agcaacggaa
     5281 atttttggcg ttgttggtat ggcaagtaaa agtgctatca aagataactt tcaagcactt
     5341 ttgcgtaagg aaaactacgc aaaaggtgtt gttgtgaggt ctagtgaagc gggcatcgca
     5401 gtagatgttt atactgtcat gagttacggc gtcaaaatta gtgaagtttc aaaaaatatt
     5461 caagagcgag tgaaattcaa tctcgaaaat cagcttggtt tatctgctga tatggtgaat
     5521 gtttatgtgc aaagtattaa ggttgtagga gaagattagt gtcaaagatt acgacaagtt
     5581 tattccaaga aatggtacaa gctgccagta cacgtctgag taaacaggca gagtatgtca
     5641 actcattaaa tgtattccca gttccagatg gtgatactgg tacgaatatg gggatgacta
     5701 tggaaaacgg agccaaggaa gtttcggaca aaccagcgac gactgttgga gaagttggtc
     5761 aaattctttc taaaggtgta ttgatgggag cacgtggaaa ttcaggtgta attacatcac
     5821 aattattccg tggttttggt caaagtatca aggataaaga agaattaacg ggtcaagatt
     5881 tggcacacgc tttccagaat ggtgttgaag tagcttataa agccgttatg aaaccagttg
     5941 aaggaactat cttgacagta tcacgcggtg cagctaccgc tgctctaaaa aaagctgaag
     6001 aaactgatga tgcagttgaa gttatgcgcg caactctcaa aggtgctaag cgtgctcttg
     6061 ctaaaacacc ggatatgcta cctgttctta aagaggttgg tgttgttgat tctggtggtc
     6121 aaggactcgt ctttatttac gaaggtttcc tctcagcact gactggggaa tatatcgcat
     6181 cagaagattt taaagcaact cccgcaacaa tgactgaaat ggttaatgca gagcaccata
     6241 aagctgtagt aggccacgta gccacagaag atattaaata tggctattgt acagaagtca
     6301 tggttggttt aaagcaagga ccaacttacg ttaaggaatt taactacgaa gaatttcaag
     6361 gttatcttag caatctaggg gattctttac tagttgttaa tgatgatgaa attgtcaagg
     6421 ttcatgttca tacagaagat ccaggtcttg ttatgcaaga aggtttgaaa tatggatcgc
     6481 ttgtgaaggt taaagttgaa aacatgcgta accaacatga tgcccaaatg caaaaggttg
     6541 aagttgaaga aactgtcaaa gaaactaaag aatatggtat tattgctgtt gtagctggtg
     6601 acggtctagc tgagattttt aaatcacaag gtgttgatta tatcatctct ggcggccaga
     6661 ctatgaaccc atctacagaa gatattgtaa aagcgattga aaaagtaaac gctagaaatg
     6721 ttattatttt accaaacaat aagaacatct tcatggctgc acagtcagct gcagatgtcg
     6781 ttgatattcc agcagcagta gtagagacac gtacagttcc acaaggattt actagcttac
     6841 ttgcttttga tcctgctaag tctcttgaaa ctaatgtagc tgatatgaca aatagcctct
     6901 cagatgtcat tagtggtagt gtgactttag ctgttcgtga tacaacgata gatggccttg
     6961 aaattcatga aaacgacatc cttgggatgg ttgatggtaa aattctagta tctacaccag
     7021 atatggaaaa agcacttaaa gacacttttg ataaaatgat tgatgaagat agtgaaatcg
     7081 tgacaatcta tgttggtgaa gatggtaaac aagcattagc cgagaccttg tcggaatatc
     7141 ttgaggagac ttatgaagat gttgaagttg agatccacca aggagatcaa cctgtatatc
     7201 catacctcat gagcgttgaa taattgataa aataaaaaat taggagagat acttctaatt
     7261 ttttatttta tcagagtttc tattttagta tgataaagca tcagtatttt gaaaattttg
     7321 acaaattgtg ctagaattgt tttaaaacat tcatacaaag gagatttatg atgtcattaa
     7381 ttattcttac ggttattttg gttttagtga ttgttctgtt gataacttct ttatacgttg
     7441 ttaaacaaca gacggtagcc atcattgaac gctttggtaa atatcaaaag actgctacta
     7501 gcggtattca tattcgtgta ccattgggta tcgataagat tgcagcacga gtgcagttac
     7561 gtcttttaca gagtgaaatt atagttgaga caaaaacaaa agataatgtt tttgtcactt
     7621 taaatattgc tacacaatat cgtgtcaatg aaaataacgt tacagacgct tattacaaat
     7681 taataaagcc agaagctcag attaaatctt atatcgaaga tgctttgcgt tcatcagttc
     7741 ctaaacttac attagatgag ctattcgaaa agaaagatga aatagccttg gaagttcaac
     7801 atcaagtggc agaagaaatg tcaacatacg gctacatcat tgttaagact ttaattacta
     7861 aggttgagcc agatgcagaa gtaaaacaat ccatgaacga aatcaatgct gcacaacgta
     7921 aacgtgtagc agctcaagaa ttagcgaatg ctgataagat caaaattgta actgctgcag
     7981 aagcagaagc agaaaaagat cgtctacatg gtgtgggtat tgcgcaacaa cgtaaagcaa
     8041 ttgtagatgg gttagctgat tcgattcaag aattgaaaga tgctaacgtg acattgacag
     8101 aagaacaaat catgtctatc ttgttaacaa atcaatattt agatacttta aatacatttg
     8161 ctattaatgg taatcaaacg attttcttac caaataaccc agaaggtgtt gaagatattc
     8221 gtacacaagt tttatctgct cttaaaacaa gataatgaac tttttataga agcaaaataa
     8281 ccagagcatt tgctctggtt attttgtgtc tgacttattt attataattg gagagacgga
     8341 aaaaagttat tttttaccgt aacgtttgta ctcatgaatg attttcagag cacgttttct
     8401 agttttaatg ttaggacttt taagtctgcg ataggctgac gataggtctg ttttttcagc
     8461 catgattacc tcctatagac aatttattaa cgttaacaga caccagtata ccacaaaaaa
     8521 attaactggt aaagtacttt gttgataaat ttaaaaataa atagcaaagt aattaaatat
     8581 caatcaaaag taataagttc aaagatatat taaaccttta ttaatgaggt ttagatttga
     8641 caagtgatgt taagtgaaag atgaatgagg aaaataccca acgagaaagt agctaatggc
     8701 tatcattgtg catataaaaa aacgatcgat gagtgatcgt ttttcagcaa actaaataat
     8761 tatttttaat gtaattgaat gaaaatttat acttgtgaaa gtgtatcggt taagttaata
     8821 aggtcagttt ttatttatcg taagcactta ggaagtgttt tggagcgtat ccacgagagt
     8881 agataaaccc ggcatcgcgt gaaggatagc ttgattcaga aggcgcatac ccacgagagt
     8941 agataaatcc ggcatcacgt gaaggataag acactgtgtc tgccttcgca agacttgctg
     9001 aagtagctaa taatagagtg acagctgaag ctgctgttaa aacactgatt ttaagtttca
     9061 ttataaaatc ctttcaaaga taataaacta ttcttgcaca gtggttagtt cattttgtgc
     9121 atactatatt atatcatcaa aagctcatta aaacaacatg tttttaattt taaacaaata
     9181 tataattaat taaatatcga ttaaaattaa aaaatgagat ttagtaactt atttaatagt
     9241 atacaatcca ataacgactg tctaatttga tctatagttg aggctagtcg tagatttcat
     9301 caaagatgtg gtaataaaaa atcccctcca gttccattgt aatgatgaca atgacaaaac
     9361 ctgtaggggt ataaatagtc tatatttaaa tgtggataaa ttttatttta agaagcgttg
     9421 tagaaattcc ttagttcttt cttgtgttgg attttcgaaa agttgcttag gagtaccctg
     9481 ctctgcaatg attcctttgt ccatgaaaat aacgcggtct gatacttctt tagcaaattc
     9541 catctcgtgt gtcacaatta tcattgttaa tccagattta gctagatctt gcattgtttt
     9601 aaggacctca ccaaccattt ctggatccaa ggctgaagtt ggttcatcaa aaagtatagc
     9661 ttctgggtta acagaaagtg ctcgggcgat tgcgacacgt tgcttttgcc cacctgataa
     9721 ttgttttggt ttagctttcc aatattgctc agtcatgcca acagcattta agttttcttt
     9781 agcaatcttt tcagcttctt ggcgttcacg ttttagaaca gtagtctgtg caacaatagc
     9841 gttctctaag atgtttaaat tctcaaagag gttaaaagat tgaaagacca tcccaagttt
     9901 ttcacgataa ttatttaaat catagccttt ttcaagaaca ttgtgaccgt gatataagat
     9961 ttctcctcca cttggttctt ctaagaggtt aatagaacgt aggaaagttg attttcctga
    10021 tccagaagaa ccaatgatag aaataacttc gcctttgttg accgaaagcg aaatgtcttt
    10081 taacacttca ttgcttccgt aagatttttt caaatgttta atttctaaaa tagcttgtgt
    10141 cataccaacc tcctagactt gtaattgatt cgcaccggtt gtgtaattat ctgaatcaaa
    10201 acgtttttca atgtaacgta agatacgtgt tacggtgaag gttaagatga agtaaataat
    10261 cgcaataatg gtgaatgttt ggaaatattg gtaggtttgt gttgcaacag tattaccaga
    10321 gaagtagagt tcgacaacag agataacgtt cagtacagag gtatctttga tattgataac
    10381 aaactcatta ccagtagctg ggagtatgtt acgtacaact tgtggtagaa caatttttcg
    10441 catggtttgt ccatgggtga aaccaagtgc agttgcggct tcaaattgtc ctttatcaac
    10501 agagaaaata ccaccacgta caatttcgct catataagca ccagtattga ttgaaacaat
    10561 aaatatagct gctagtgtac ggtccagcga aactccgaaa gcttgtgctg ttccgtagta
    10621 gataaccatt gattgtacaa tcataggtgt cccgcggaag acctcaatat agatgttaag
    10681 taaccaacct aataattttt gtaaggcagc ctttaactta ttgtcagatt ttggtgctgt
    10741 acggaaaaca ccgatgagaa ggccgataaa taatccgacg atagttccta ccatagaaat
    10801 taggagggtt gtagctgttc ctctgaggaa ttgagaacca ttgtttttta aaatagttgc
    10861 catctgttca aagaagtttg gttttccatt tttttctttt ttaacggatg gttgttcttt
    10921 aatcatctta tccatcaagg cgatttgttt atctctagaa atgctttcga gaacttgatt
    10981 gacttgatta atacgattgt cacctttacg catcccaacg gaaatatttg tatcggcatc
    11041 agatgtttta aacccttggt gcagttcaat catttttaat ttaggattag cagtttgagc
    11101 ggaagttgca tctggacgtt ctgaaacata ggcgtctaca acaccagatt ctacagcttg
    11161 tcttaattga ttaaagtccc ccatggcaac ttcttttttg acaccattga tttgatcaat
    11221 gaggttgtat aagtagactc cttgttgggc agttacttta gcatttttga aatcactaat
    11281 atttttagca ttagtatatt ttccttcagc gttaacaact agagtaggtt ctgaaatgta
    11341 gtaaggcttt gaaaaattaa tttctttttt acgttcttcg gtaggactca ttcctgcaat
    11401 aatcatatcg agttttccag aagttagtgc tggaacaagg ccttcccatt ttgttttgac
    11461 aacaacaact tttttattta actttttagc taacttttta gcgacttgta catcataacc
    11521 attagcatat tgatcagttc cttcaatagg aactgcacca tttgtgttat cattttgtgt
    11581 ccagttgaaa ggcgcatagg ctgcctccat accgacacgt agatattcat ctgcttgtat
    11641 acttgtcata cctccaaagg tgataagcaa agcagtgaat aaacttagta atattttttt
    11701 cattcaacag cctccgataa gttacttcct cctattttac cgaaaaacaa ggtcgatttc
    11761 aatatcatta tcttattgaa agcgattaat tgtattttta tgttaaaatg gtatacaaac
    11821 aaaggaggga ggatgataat gaagaaatgt tttttggcta tttgtttagc tcttagtttt
    11881 tttatggttt cagttcaagc agatgaggtg gactataaca ttcctcatta tgagggtaat
    11941 ctaactattc acaatgataa tagtgctgat tttacagaga aggttactta ccaatttgat
    12001 tcgtcctata atggacagta tgtcacgtta ggtacggcgg gtaagttacc tgacaatttt
    12061 gatattaata ataagccaca ggttgaagtt tcaattaatg gtaaagtaag gaaagttagt
    12121 taccagatag aagatttgga ggatggctac cgtttgaaag tgtttaatgg tggtgaagca
    12181 ggtgatactg ttaaagtcaa tgttcagtgg aaactaaaaa atgttctatt tatgcataag
    12241 gatgttggtg aacttaactg gattcctatt agcgactggg ataaaacgtt agagaaagta
    12301 gatttttgga tatcaactga caaaaaggtt gctctttctc gtctttgggg gcacttgggt
    12361 tatcttaaaa ctcctcctaa aataagacaa aataataatc gttaccattt gacagctttt
    12421 aatgtaaaca aacgattaga atttcatggt tattgggata gatcttattt taatctacct
    12481 acaaacagta aaaataatta caagaaaaaa attgaacatc aagagaagat aatagagcgt
    12541 catggtttta tcctaagttt cttgttaagg atattattac cttcattctt tattattgtg
    12601 acactattca tctcaattag ggtgttcctg tttagaaaaa aagttaataa atacgggcaa
    12661 ttccctaagg atcatcattt atatgaagca cctgaggacc tttcaccatt agagttaact
    12721 caaagcattt atagtatgag ctttaaaaat tttcaagatg aggagaagaa aactcacctt
    12781 atcagtcaag aacaactcat acagtcaatt ctattagact tgattgatag aaaagtattg
    12841 aattatgatg ataacttgtt atctctagct aacttagata gagcttctga tgcagaaata
    12901 gattttatag agtttgcttt tgcggattct acgagtttga agccagatca actcttttct
    12961 aattaccaat ttagttataa agaaacacta cgtgaactga aaaagcagca caaggcttca
    13021 gatctgcaaa atcaaatgag acgccgagga agtaatgcct tatcaagaat tacgcgtctc
    13081 acaaggttga tttctaaaga caatataaac tctcttagaa gaaagggaat ttcatcccct
    13141 tatcgtaaaa tgtcttcaga agagtctaaa gaattatcta ggttaaaaag attcagttac
    13201 ctatcacctc ttatttcttt tgttgttata atttatacgc tttttttaaa ttattttacc
    13261 tatttctgta tctatctctt attgtttggt gttatcctgt tgttgaataa aatcattttt
    13321 atgatgacaa gaaaaataag taacggttat attgtaactg aagatggagc aagtcgtgtc
    13381 taccaatgga ctagttttag gaacatgcta agggatatca aatcgtttga tcgttcagag
    13441 ttagaaagta tcgtattatg gaatcgaata ttggtttacg ctactttatt cggctacgct
    13501 gaccgtgttg agaaagtact cagagtgaac caaatagata ttccagaaag atttgcaaac
    13561 attgatagtc atcgatttgc gatttcagtc aatcaatcta gtaatcattt ttcaacgata
    13621 actgaagatg ttagtcacgc ttctaatttt agtgttaatt caggcggttc ttcaggtggt
    13681 ttctcaggcg gcggaggcgg cggaggtggc ggtgccttct aattttccaa aagtatggta
    13741 taataaagaa aaatttatgg agttattatg ttaataattg aattactcaa agccctattt
    13801 ttaggagtcg ttgagggagt gacagaatgg ttacctgttt ctagtactgg ccatttaatc
    13861 ttggttcaag agtttatgaa gttgaaccaa agcaaatcct ttgttgaaat gtttaatatt
    13921 gttatacaac ttggggcaat tatggcagtt attgtcattt attttaaacg ccttaaccca
    13981 tttcagccag gtaaatctgc tagagaaatt cgtttaactt ggcaattatg gttaaaagta
    14041 gtgatagctt gtattccatc tattttaatt gctttaccat ttgataattg gtttgaagct
    14101 cattttaatt tcatgattcc gattgcaata gccctaatct tttatggttt tgtcttcata
    14161 tgggttgaaa aacgtaatgc acacctcaaa ccacaggtaa ccgaattggc aagtatgtct
    14221 tacaagacag ctttcttgat tggatgtttc caggttctca gtattgttcc gggaaccagt
    14281 cgttctggag ctactatttt aggagcaatt attattggaa ctagtcgttc ggtcgctgct
    14341 gactttactt tcttccttgc catcccaact atgtttggtt atagtggact taaggcggtt
    14401 aaatattttt tagatggtaa cgtcttgagt ttagaccaat ctttaatact tttagtagca
    14461 agtctgacag ctttcgtagt tagtttatat gttattcgtt tcttgacaga ctatgtcaaa
    14521 cgacacgatt tcaccatctt tggtaagtat cgtatagtct taggaagttt actcatcctc
    14581 tactggttag ttgttcattt attctaaaat ataacataaa aagctgagta ttcctcagct
    14641 ttttagcgtg ttaggctgtg aaaaattgct aaaaaatagc tttttcggta taataagaaa
    14701 actgaatact cgtatgaggt gatgatatgg aaatgaaaca aatcagtgaa acaacactga
    14761 aaattacaat tagtatggaa gatttagaag atcgtggtat ggagctgaaa gatttcctaa
    14821 tccctcagga gaagactgag gaatttttct attctgtcat ggatgaatta gacttgccag
    14881 aaaactttaa aaatagtggt atgttaagtt ttcgagtaac acctaaaaaa gatcgcattg
    14941 atgtttttgt tacaaagtct gaattaagta aagatttaaa tttagaagaa ttagcagatt
    15001 tgggtgacat ttcaaaaatg tctccagaag acttttttaa aaccttggaa caatcgatgt
    15061 tggaaaaagg ggatacggat gcccatgcca aattagcaga aattgaaaat atgatggata
    15121 aagcaactca agaagtagtt gaggaaaatg tttctgaaga acaacctgaa aaggaagtag
    15181 aaacgattgg atatgttcac tatgtctttg attttgataa tattgaagct gtagttcgat
    15241 tttcacaaac gattgatttt ccaatagaag cttctgaact ctataaaaat ggtaaaggct
    15301 atcatatgac aattttgcta gacttggaaa accaaccaag ctactttgct aatttgatgt
    15361 atgctcgaat gcttgaacat gctaatgtgg gcacaaaaac acgagcttac ttgaaagagc
    15421 atagtattca attgattcat gatgatgcta tttcaaaatt acaaatgatt gagatgggat
    15481 aaaatgatac cttttacaat tgagtatatt tttgtattga ttggagcatt tttattatca
    15541 atcattctaa ctcctattat tcgagttata tctttgaagg taggagcggt tgataaaccg
    15601 aacgctcgaa gaatcaacaa agtaccaatg ccaagtagtg gtggtttagc aatatttctc
    15661 tcctttgttg taacaacatt atttttcatg ccaatggcgg ccagtagaca ctttatagaa
    15721 gtctcttatt ttcactacat tttgcctgta attattggcg gtctcgtagt tacaacaaca
    15781 ggatttatag atgacatctt tgaattaaga cctcgttata aaatgttggg aattattatt
    15841 gcagctatta tcatatggaa atttacacat tttagatttg atagttttaa gattccaata
    15901 ggtggtccac tcttggagtt tggtcctatt ttaactttct ttttgacagt tttatggatt
    15961 atttcaatca ctaatgctat caatctgatt gatggtctag atggtctagt tagtggtgtt
    16021 tcaatcatca gtctagcaac gatggcagtg gtttcttatt tctttttacc taaaatagat
    16081 ttttttctca cgctaactat tgttatttta atcgcctcaa tagttggttt tttcccttat
    16141 aattatcatc cagccattat ctacctaggt gatgctggtg ccttgtttat aggatttatg
    16201 attggtgtat tatcgcttca aggtttaaaa aactccacag ctgttgcagt gataacacct
    16261 gtcattattt taggagttcc tattttggat acagctgttg ctattgtgag gcgtaaatta
    16321 tcagggaaaa agatttctga agctgataag atgcacctgc atcaccgatt gctatctatg
    16381 ggatttacac atagaggagc agttttagtt gtttatggta ttgcaattat cttttcgctt
    16441 atcgctttgc ttttaaatgt ttctagtcgt attgggggaa tttttcttct attggctcta
    16501 ctattagtaa tggaaatttt tatagaaggt ttaaatattt ggggtgagaa tcggacaccg
    16561 ctatttaatc tcttaaagtt cattggcaac agtgattaca gacaaagtgt tattgctaaa
    16621 tatagcgata agcatcaaaa ataaaagcct gaaaaggctt ttttagtttt aatcatttag
    16681 atagcagtca cacctcattt ttgctataat tatttagaat ataatatcgg gtgagtgccc
    16741 tataatatct tctttaaacc atagagaaat atcacatatt ctctgttaac caagaaaagg
    16801 agtgtacaat gtctgtactt gaaatcaaaa atcttcatgt ttctattgaa gataaagaaa
    16861 ttttaaaagg tcttaattta acgctgaaaa caggagaaat tgctgctatc atgggtccaa
    16921 atggtactgg gaagtcgaca ctttcagcag ctattatggg aaatcctaat tatgaagtta
    16981 cagcaggtga aattcttttt gatggtgaag atattttaga gctagaagtg gatgaacgtg
    17041 ctcgtttggg tctatttctt gcgatgcaat acccttcaga ggttccaggg attactaatg
    17101 ctgaatttat ccgagcagct atgaatgcgg gaaaggctga tgatgataaa atttccatcc
    17161 gccaatttat tactaagtta gatgaaaaga tggaactact tggcatgaaa gaagaaatgg
    17221 cggaacgtta tcttaacgaa ggtttttcag gtggtgagaa gaagcgcaat gaaattttac
    17281 aactcctaat gttagagcct aagtttgccc tcttggatga gattgactct ggcctggata
    17341 ttgatgctct taaagtggta tcaaagggag tcaatgaaat gcgtggtgaa ggttttggtg
    17401 ctatgattat tacacactat caacgccttt tgaattacat tacaccagat aaagtacacg
    17461 ttatgatgga cgggaaggta gtattgtcag gaggaccaga attagcggtt cgccttgaga
    17521 aagaaggtta tgctcaaatc gctgaagagc ttggtttaga gtataaagaa gaagtctaat
    17581 ttgtcacttc aactagaaga gaaaaggagt tacaaatgtc taaagaagct atattaaatt
    17641 ttttacaagc caaaggtgag cctacttggt tacaagaatt acgactaaag gcttttgaaa
    17701 aaatagaaga actagaatta ccagtaattg aacgtgtaaa atttcataga tggaatttag
    17761 gtgatggaac tattttagag aatgactata cagctaacat tccagatttc acagagttag
    17821 gaaacaatcc caagttggtt caaattggga cccaaactgt ccttgaacaa gttcctatgg
    17881 aattaataga gaaaggcgtt gtctttacag atttctattc ggctttggaa gaaattccag
    17941 aagtcataga gcgctacttt gggaaagcac gtccttttga agaagataga cttgctgcct
    18001 atcacacagc ttacttcaac agtggtgcag tgctttatat tccagataat gttgaaatta
    18061 ctcaaccaat cgaaggtctt ttttatcaag atagtcaatc taaagtacca tttaataagc
    18121 atatccttct tattgttgga aaaaatgcaa aagttagtta cttagagcga tttgagtcaa
    18181 ttggtgatgg gacagaacga acaagtgcta atatttctgt agaagtaatt gcccaagcag
    18241 gtagccaaat taaatttgcc tcaattgatc gcctcggaga aaatgtgaca acgtttataa
    18301 gtcgtcgtgg tcgtcacagt agcgatgcaa caatagattg ggcccttggg gtgatgaatg
    18361 agggcaatgt tgttgctgac tttgattctg atttaattgg tgatggaagc cacgctaatt
    18421 taaaagtagt tgctgcttcg tcagggcgtc aagttcaagg aattgatacg cgtgttacaa
    18481 actacggctg taattcagta ggacatattt tacagcatgg ggttatttta gagcgtggga
    18541 ctcttacttt taacggtatc ggacatatta taaaaggtgc aaagggagca gacgcccaac
    18601 aagaaagccg agttctcatg ttatcagaca aagcacgttc agatgctaat cctatccttt
    18661 tgattgatga aaatgatgtt acagctggtc acgcagcctc aattggtcag gttgacccag
    18721 aggatttata ttatttgatg agccgtggct tgaaccaaaa aacagcagag caattagtta
    18781 ttcgtggatt tttaggaaca gtcattgctg aaataccagt aaaagaagtt cgcgatgaaa
    18841 tgattgccgt tattgataca aaactagaaa aacgttaata tgacattact agatagttat
    18901 aaactaaagc aagatttccc aattttaaat caattagtta atgatgagcc gcttatctat
    18961 ttagataatg cagcaacgac acaaaaaccg aatcaagttt tagaaacttt gcgagattac
    19021 taccaaaatg acaatgcaaa tgtccatcgt ggtgttcata ctttagcaga acgagcgact
    19081 gctcagtacg agactgctcg tgaaaaagca aggcagtttt taaatgctaa atcaagtaaa
    19141 gaaattttat tcacacgtgg caccacaaca ggattaaatt gggtagctaa gtttgccgaa
    19201 agtattttag aacgaggtga tgaggtactt atttccatta tggagcatca ttctaatatt
    19261 atcccatggc aacaagcttg cgagagaact ggtgctaaat tggtttatgc ctatctaaaa
    19321 gatggttctt tagatttaga agatttctat aataaattat cctctaaaac taaatttgtt
    19381 agtttggcac atatctcaaa tgtacttggc tgtattacac cagtaaaagc aatagcagaa
    19441 cgtgtgcatc aagttggggc atacatggtt gttgatggag ctcaatcagc gccacatatg
    19501 gcgattgatg ttcaagacct tgattgtgat ttcttcgcct tatctggcca taagatgctt
    19561 ggtcctactg gtattggtgt tctttatggg aaagaaagca ttttagataa gatgccacct
    19621 gtcgaatttg gtggtgaaat gattgatttt gtctatgagc atagtgcaac atggaaggaa
    19681 ttgccctgga agtttgaagc tggaacgcct aatattgctg gagcgatcgc ttttggagaa
    19741 gctttagatt atttaacgga tgttggtatg gatgagattc atcaatatga acaaagttta
    19801 gtttcttatg tgttgcctaa attacaggct atcgatggtt taactattta cggacctagt
    19861 gatgctgaat ctcatgtagg cgttattgcc ttcaatttag aaggtttaca cccacatgat
    19921 gtggcaactg caatggatta cgaaggagtg gctgttcgtg caggtcatca ttgtgcacaa
    19981 ccccttatta atcatcttgg tatccattcg gcggttcgag caagtttcta tttctataat
    20041 accaaagagg attgcgacaa attggtagac gctatccaaa aaactaagga gtttttcaat
    20101 ggcactctct aaattagata acctctacat ggcggtggta gctgatcatt caaaacaccc
    20161 tcatcatcat ggatttttag aaggcgttga acaagtgcag ttaaacaatc ctacatgtgg
    20221 agatgttatt tccttatctg ttaaatttga tgggaatatt atttcagata ttgcatttgc
    20281 tggtaatggt tgtacaattt ctacggcttc atcaagcatg atgacagatg cagttattgg
    20341 gaaaacaaaa gaagaagcat tgcagcttgc tgatgtattc tccaaaatgg ttcaaggcaa
    20401 tcaaaatcca aaacaagaga aacttgggga tgcagagttt ctagctgggg tatcaaaatt
    20461 tccacaacgt atcaaatgtg ctacgctttc gtggaatgct ttaagaaagg ctattgagag
    20521 agataatcag gcagaactct gacaaccata gttgaaggaa ttacatttaa acaactttta
    20581 ctgacataag gtagtctagt agaagaatgt cttaaattta aatattaaag aaaggataag
    20641 tatgtccgaa ataaatgaaa aagtagaacc acagccaata gatcttgggg aatataaatt
    20701 tggatttcat gacgatgtaa agccaattta ttctacggga aaaggtctaa atgaggctgt
    20761 tattcgtgag ttatctgcag ctaagggtga acctgagtgg atgttggact ttcgtctaaa
    20821 atccttggaa acgtttaata aaatgccgat gcagacctgg ggagcagatt tatcagatat
    20881 tgattttgat gatattattt attatcaaaa agcatctgat aaacctgcgc gtgattggga
    20941 tgatgttcca gaaaaaatca aagaaacttt tgaaagaatt gggattccag aagctgaaag
    21001 agcctatctt gcaggagcat cagcacaata tgaatcagaa gtagtttatc acaatatgaa
    21061 agaagaatat gataagctgg gtattgtttt tacggatact gactctgcac ttaaagagta
    21121 cccagagcta ttcaaaaaat attttgctaa acttgtccct ccaacagata ataaattagc
    21181 tgctctgaac tctgctgtat ggtcaggtgg aacatttatt tatgttccta aaggtgttaa
    21241 ggtggatatt ccacttcaaa cttacttccg tattaataat gaaaatactg gacaatttga
    21301 acgtactctc attattgttg atgagggagc aagtgttcac tatgttgaag gttgtaccgc
    21361 cccaacttat tcttcaaata gtttacatgc agctatagtt gaaatttttg cacttgatgg
    21421 agcttatatg cgctatacga ctattcaaaa ttggtccgat aatgtctata atttagtgac
    21481 aaaacgtgct accgctaaaa aagatgcaac agttgagtgg atagatggaa atctaggagc
    21541 taaaacaaca atgaaatacc catcggttta ccttgatggt gaaggagcac gtggcacgat
    21601 gttgtctatt gcttttgcaa acaaaggaca acaccaagat acgggtgcaa agatgattca
    21661 taatgccccc catactagtt catccattgt ctctaaatca attgctaagg gtgggggaaa
    21721 agttgattat cgaggtcaag tgacatttaa taaagattcc aaaaaatcag tgtcacatat
    21781 agaatgtgac accatattga tggatgatat ttcaaaatca gataccatac cgtttaatga
    21841 gattcataat tcacaggttg ctttagagca tgaagcaaag gtgtctaaga tttctgaaga
    21901 gcaactgtac tacttgatga gtcgaggttt atctgaagct gaagcaacag aaatgattgt
    21961 tatggggttt gttgagccct ttacgaaaga attaccaatg gaatatgcgg tagagttaaa
    22021 tcgtttaatt tcctatgaaa tggaaggttc agttggttaa ttgaagattt caagagataa
    22081 taaaaagccc ctatgttatt gatgaagggg cttttatatt agcacagtta ttatctagag
    22141 cgctttataa gtaatactat cccagctaaa agacaaaggc tagcgatggt gcccagaaca
    22201 attaaaatag atacaaaatg tgattggata aattgatctg tttttgtcca ccactgttct
    22261 tttgtttgag gctgttcttg aatagattta gttttggttt tagtaacttc ttttttaggt
    22321 ttaacggctt ttaatacagg agttttagaa gaaatatttt tagcgtcttt aaaacctttt
    22381 tctacaagag cgttgacaaa cggatgacga tagtactctc cgtcttggtc tgaccaatcg
    22441 ccaactccta aaaccacagt tatcaagcga gtattctggc gtttagctgt aactaaggca
    22501 ttaaaagcag cgctagggct agaaccagtt tttaagccat ctactccttc taatcccaat
    22561 ttagcgccgg gggtagagta gttataagtt gtaaatgttt cttcataagg agttccgacc
    22621 atggccttga cttcaggata ttttgtatag ttcagtatat cagggtattt tttaaggaaa
    22681 tgataggtta aaattgatag atcacgtgca gtcgtaacgt tagtagcatt gttatcgtat
    22741 tcttttgggg agtaaagtcc attaaaagca ctcgctaccg ccccactggg gttataaaag
    22801 tgagtttttg tcataccgag tttcttggcg gtttcattga ttcgtttaat aaaggcgtca
    22861 ggattgtttt gtgataagtg gttagcaatc ataatagttg ctacattaga tgacgggaca
    22921 gccgtcatag taatcagttc acgaatagga taagcaaccc cagcatgaat attgttatta
    22981 ctaatttcat aaatcttact tatggcttgg tctgtttctg ttgcagtcac tgtggtgttg
    23041 aggtttgttt ttcctttagc taagtcttca aatagtaaat ataaagtaaa cattttagac
    23101 atgctagcgg gatcacgttc taaatcggcg ttatcttccc acaaaatatg acctttattg
    23161 tcaataacga tagacgcttt aggtttatta atatctttaa catcatatcc ggcttctcgg
    23221 gtaatttgca taatatcttt atgttcttca gcttgcacag agtttacaca agtcagggat
    23281 aataaaatgc agaggaaaga tacgattaat ttaggcatag gtaactcctg aaaacttttt
    23341 attaatatta taacaaaaaa tcactaataa aaaagacatt tcattagtga tttttaattt
    23401 tatatattct cctaaaccta gctaaagaaa ctgctgtgca gtaactcctt gtgtcaaagg
    23461 gagattacta ttaaagtttt tcgttaacgt agcgcacaaa atgattccac cacactttga
    23521 ggaagaaact gcgctcaata ctatcttttg ccactaaatg aacgctaggt tcctttatga
    23581 gatagccttt tccaacctta ttaaggtcaa cgtaataagc tttccctagg ttctcttttt
    23641 ttgtaatagg agcagtaagc tcttttttaa agttaatttt taactgattt tgtttagtga
    23701 tatttttctt ctgtactact ttcaaatcgt ttttagcaac agcgatgacc gattgttctt
    23761 ttccgtctct cacacttgct tctttacctt tatatgcagc tcctttagct aatacaaggt
    23821 taggttcgta ggtgtttgtg atatagttca agagagagtt agttgctgta aagcgagcat
    23881 atttgtcttt atcggcctta tcagcatgca taataacagt gagtagtctc attccacttt
    23941 cagtagatgt agcaataaaa gattggccag ctaattccgt tgtcccagtt ttcaaacctg
    24001 taatacctgg tctaaagaca ggcatatctg gtagcatgta gttataagaa tgcataatat
    24061 ctttatcaaa tttagcaacg gacttactag taatcttaag aatggaagga tattcgttga
    24121 ccaaatggta ggcagcaata gcaatatcac gtgcactcat tttattttcg tcgttttgtg
    24181 acgattttgg ataaatgtga ttgcctaaca tactattatt taagccagaa gcattgacta
    24241 ggtggctatc atgaattccc cacttttcca attgagcagt cattttatca acaaatttac
    24301 tttcagttcc tgaaatatgt tcagctaaag caatggctgc actgttagca ctagaaatca
    24361 tggcagcgtc cacgagttgt ttaacagtat agcgcctttt ttctaaagga acattactag
    24421 catcagattc gcgtgttagt tgataagggt agtcagatat atttactttg gtattccact
    24481 tgaggttacc gttatcaatt tctttataga ccatataaac ggtcattatt ttagtcaagg
    24541 aagcaatagc agcgggtttg ttagcatctt tttcatacaa gatttttcct gaatctaaat
    24601 caactgctaa ggcatgttta gcggaagcat taaaagattc agcagagatg gttggcataa
    24661 aaaaaatgca actaagtaat agaatagaag taataatttt tttcaaaata acacctttag
    24721 aaatttttta aactttgtcc ctcctattat accatatcac tatgtcatac taggaattat
    24781 tattttcaga taattgaatg aagaaaacgt ttgttataaa aatgtaaaaa aatgtaaaaa
    24841 aatgaaagtt aaaaaattat ctgaaaattt tgcgtttttg tcttttctta agaatttttt
    24901 gtggtataat gttagtcact agataaatga attaggagaa caaagctatg gcaaaacaga
    24961 aaaataactg gcgccgtgtt ggagttggtg tccttacact tgcttcagtt gcgactcttg
    25021 ctgcatgtgg aagtaaatca gcttcccagg attctaatgg agcgattaat tgggctattc
    25081 caacagaaat caatacacta gatttatcta aagttacaga cacttactca aatctagcta
    25141 ttggtaactc tagtagtaat ttccttcgct tagataaaga tggaaagaca agaccagact
    25201 tggctactaa agttgatgtt tcaaaagatg gcttaactta tacagctaca ttacgtaaag
    25261 gcttgaagtg gtcagatggc agtaaactta ctgcaaagga ttttgtttat tcatggcaac
    25321 gtttagttga tcctaaaaca gcttcacaat atgcttacct tgctgttgaa gggcatgtgc
    25381 ttaatgccga taaaatcaac gaaggacaag agaaagactt gaataagcta ggtgttaagg
    25441 cagaaggcga tgacaaagtt gttattactt tatctagtcc gtctccgcaa ttcatctact
    25501 accttgcatt cactaacttc atgccacaaa aacaagaagt tgttgaaaaa tatggaaaag
    25561 attacgcaac tacttcaaaa aatacagttt actcaggacc atatactgtt gaaggttgga
    25621 atggttcgaa tggtactttc acgctgaaga aaaacaaaaa ttattgggac gctaaaaatg
    25681 taaaaacaaa agaagttcgc atccagactg ttaaaaaacc agataccgcc gttcaaatgt
    25741 ataaacgtgg tgagttagat gcagctaata tctcaaatac ttctgctatt tatcaagcta
    25801 ataaaaataa taaagatgtc acagatgttc tagaagcgac cactgcctat atggaatata
    25861 atactactgg ttctgtgaaa gggcttgata atgttaagat tcgtcgcgcc ttaaacttag
    25921 caactaaccg taaaggagtt gttcaagcag ccgttgatac aggctcaaaa ccggcaattg
    25981 cttttgcacc tactggttta gccaaaacac cagatggaac tgatttggca aaatatgttg
    26041 ccccaggtta tgaatataat aaaactgaag cagcaaaact ctttaaagaa ggtttggctg
    26101 aatcaggctt gactaagcta aaattaacaa ttacagcaga tgctgatgct cctgctgcca
    26161 aaaactctgt tgactatatc aagtctactt gggaagctgc tcttccagga cttactgttg
    26221 aagaaaaatt tgtaaccttt aaacaacgct tagaagacag tagaaaacaa aactttgaca
    26281 ttgtagtttc tctttggggt ggggattatc cagaaggatc aactttctac ggcctcttta
    26341 agtcggattc acaaaataac gatggaaaat ttgctaacaa ggactatgat gctgcttata
    26401 acaaggcaat ttctgaagat gcgatgaaac cagcagaatc agcgaaggac tataaagaag
    26461 cagagaaaat tttatttgag caaggtgctt ataacccact ctacttccgt agtggtaaag
    26521 gtttacaaaa tccaaaatta aaaggtgtta ttcgtaatac tacaggtttg tcaatagact
    26581 ttacacatgc ttataaaaat aattaaatta ctttacacta aacctttgca agaattggta
    26641 attgccagtt cttgcttttg gttaagtaaa gaaagtgaaa gagtttgcta tttttagcaa
    26701 ttctaagaaa aggaaatgat atgattaaat atattttaaa gcgtgttgct attttgttag
    26761 taacgctatg ggttgtcata accctatcgt tctttctgat gcagatcctt ccaggaacac
    26821 cctacaataa tccaaaactc actgaagaaa tgatcacatt gcttaataaa cagtatggtt
    26881 tagacaagcc tgtatggcaa caatatttga cttacctttg gaatgtactt catggtgatt
    26941 ttggaacgag ttatcaatca gtgaatcagc cagtatcacg tatgattagc ttgagattag
    27001 gagtttcagt acatctaggt gttcaggctc tcgtatttgg tgttttaggc ggtattttgg
    27061 taggagctat ttcggcacgt cataagaatg ataaagtcga tggtatctta agtgttattg
    27121 caacgctagg gatttctatg ccttcattta tcattggtat cttattatta gactattttg
    27181 gatttaagtg gaacttgttg cctttatcag gttgggggac tttctctcaa acaattttac
    27241 cttctttagc tctaggactc cctacattag catctgtttc acgcttcttc cgtagtgaaa
    27301 tgattgaaac tttaaattct gattatgtgc aactagctcg ttcaaaaggt atgacaattc
    27361 gtcaagtaac tcgaaaacat gcttaccgta attctatgat tccaatttta actctaattg
    27421 gccctttggc ggcgggtctg ttaactggtt ctgcactaat tgagcagatt ttctcgattc
    27481 caggaattgg gcagcagttt gttacttcga ttccaacaaa agactatcct gttattatgg
    27541 ggacaaccat tgtttatgca gtaatgttga tggttgctat cttgataaca gacgttgtta
    27601 ttagtatcgt agacccacgt gttcgcttgc agtaaggaga tgttatggca gataaaaaca
    27661 gaacatttaa acttgtaggt gcaggatctt ctagcacaca agaaaaaatt gaaaagcctg
    27721 ctctttcgtt tatgcaagat gcgtggcgtc gcttgaaaaa aaacaaatta gcagtagttt
    27781 cactctattt attagctctt ttacttactt tttcgttagc ctcaaattta tttgtaactc
    27841 agaaggatgc taatgggttt gattcgaaaa aagtaacgac atatcgcaac ttaccaccta
    27901 aattgagttc aaaccttcct ttttggaatg gtagcattaa gtacgctgga aatacagaat
    27961 ctacagacgc ttataagtct caaaatgtcc ctgaaaaagt taagtatgct ttaggtacag
    28021 attctctagg gagaagtgtt gctaagcgca tcatcgtagg tattcgaatt tccttacttg
    28081 tagctattgc agctacattc attgatttaa ttatcggtgt tacatacggt cttgtttcag
    28141 gttttgctgg aggacgcctt gatacattaa tgcaacgtgt cgtagaggta atttcatcaa
    28201 ttccaaactt agttattgtt acaatgttag gtttggtcct tggtaacggt attacggcta
    28261 ttattatctc tattgctttt accggatgga cttctatgtc tcgtcaggta aggaatttaa
    28321 ccctatcata tcgagaaaga gagtttgtgc ttgctgcacg ttcacttggg gaaagtccaa
    28381 ttaaaatagc cttcaaacat attttaccta acatttctgg tattatcatt gtacaaatta
    28441 tgatgacaat cccaagtgct attatgtatg aagcggtact ttcagcaatc aatttaggtg
    28501 ttaaaccacc gacagcttca ttaggatcat tgatttcaga tgcacaagaa aatttacaat
    28561 attatccata tcaagttatc ttgccagctt tagcattggt aatgatttca ttggcattta
    28621 ttttgcttgg tgatgggttg cgagatgctt ttgatccaaa atctagcgat gaataaggag
    28681 gtataatcat ggaaaaagaa actattttaa gtgttaataa tcttcatgtt gacttccaca
    28741 catatgctgg agaagtaaaa gcaattcgtg atgtcaactt tgaattaaaa aaaggtgaga
    28801 ctcttgcaat cgttggtgaa tctggttcag gaaaatctgt aactaccaga actttaattg
    28861 gtttaaatgc taaaaattca gagatatcag ggaatgttca atttaagggg cgtaaccttg
    28921 ttgaactatc agaagaagag tggactaagg tacgcgggaa tgagatttct atgattttcc
    28981 aagaccctat gactagtttg gatccaacga tgaaaattgg tatgcaaata gcggagccaa
    29041 tgatgattca tcaaaaaatt tcaaaaaagg atgctttaaa attagctctt gagctaatga
    29101 aagatgtagg tatcccaaat gctgaagagc atattaacga ttacccacat cagtggtctg
    29161 gaggaatgcg tcaacgtgcg gttatagcga ttgctttagc tgctgatcct gaaattctta
    29221 ttgctgatga accaacgact gctttagatg taacaattca agcacaaatt ttaaacttaa
    29281 tgaaaaaaat tcaagcagaa cgagactcct ccattgtttt cataactcat gacttagggg
    29341 tcgttgcagg gatggcagac cgtgtagcag ttatgtatgc agggaaaatc gttgaatttg
    29401 gaactgttga tgaagtcttt tataatccac aacatccata tacttgggga ttattgaatt
    29461 caatgccgac aaccgaaaca gaatcaggta gtttagagtc aattccggga acaccaccag
    29521 atttgttgaa cccacctaaa ggagatgcat ttgcagctcg taatgaattt gcattagata
    29581 ttgatcatga agaagaacca ccgtacttca aagtttcaga aacacatttc gctgctacat
    29641 ggcttttaga tgaaagatca cctaaggtgc ttcctccatt accgatccaa aaacgttggg
    29701 aaaaatggaa cgagattgaa gggaggaaag cccaatgact gaaaatcgaa aaaaattagt
    29761 tgaagtcaaa aatgtttctt tgaccttcaa taaaggaaaa gctaatgaag tgagagcaat
    29821 tgataatgtt agttttgaca tttatgaagg tgaagtgttt ggattagttg gggaatctgg
    29881 atcaggaaag acaaccgttg gacgttcaat tttaaaactt tatgatattt ctgatggcga
    29941 aattaccttt aatggagaag tgatctcaca tctgaaaggt aaagcattac atagttttcg
    30001 taaagacgct cagatgattt ttcaggatcc tcaggctagc ttaaacggcc gtatgaagat
    30061 tcgtgatatc gtagcagaag gtttagacat acataaatta gctaaatcaa aatcagatcg
    30121 tgatagtaaa gttcaggccc tattagatct tgtagggtta aataaagatc atttaacacg
    30181 ttatccgcat gaattttcag gtggacaacg acaacgtatc gggattgcac gtgctttagc
    30241 agtagagcct aaatttatca ttgctgatga accaatatct gccttagatg tttcaatcca
    30301 agcacaagtt gttaatttaa tgcaaaaatt gcaaagagaa caaggattga cttatttgtt
    30361 tatcgcacat gatttgtcta tggtaaaata catttcagat cgtatcggtg ttatgcattg
    30421 ggggaaactg ttagaagttg gaacatctga tgatgtttat aacaaaccaa tccaccctta
    30481 tacgaagagt ctattatcag ctattccaga accagatccg gagagtgaac gtcaacgtgt
    30541 tcaccagccg tataatccag ctattgagca agacggacaa gaacgtcaaa tgcacgagat
    30601 tacacctggt cattttgttt tatctacacc ccaagaagct gaagaatata aaaaacaaat
    30661 attataaaag atggctagcc catcttttat ttttttacta ataaaataaa tttaggtatg
    30721 gaattttaaa ctctatgtta ttggtgttag ccaacaggaa act
//
LOCUS       DATRNX010000009        30198 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRNX010000009 DATRNX010000000
VERSION     DATRNX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30198
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            156..884
                     /locus_tag="VBC81_000344"
     CDS             156..884
                     /locus_tag="VBC81_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6911409.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            938..1975
                     /locus_tag="VBC81_000345"
     CDS             938..1975
                     /locus_tag="VBC81_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6911410.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            2055..2663
                     /locus_tag="VBC81_000346"
     CDS             2055..2663
                     /locus_tag="VBC81_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6911411.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            3001..3852
                     /locus_tag="VBC81_000347"
     CDS             3001..3852
                     /locus_tag="VBC81_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6911412.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            3845..5713
                     /locus_tag="VBC81_000348"
     CDS             3845..5713
                     /locus_tag="VBC81_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6911413.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            5730..7157
                     /locus_tag="VBC81_000349"
     CDS             5730..7157
                     /locus_tag="VBC81_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6911414.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(7226..8320)
                     /locus_tag="VBC81_000350"
     CDS             complement(7226..8320)
                     /locus_tag="VBC81_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6911415.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSKNDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDIYLHKL"
     gene            8486..9628
                     /locus_tag="VBC81_000351"
     CDS             8486..9628
                     /locus_tag="VBC81_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6911416.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            9653..10909
                     /locus_tag="VBC81_000352"
     CDS             9653..10909
                     /locus_tag="VBC81_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6911417.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            11010..12074
                     /locus_tag="VBC81_000353"
     CDS             11010..12074
                     /locus_tag="VBC81_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6911418.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(12129..12464)
                     /locus_tag="VBC81_000354"
     CDS             complement(12129..12464)
                     /locus_tag="VBC81_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6911419.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(12653..13681)
                     /gene="queA"
                     /locus_tag="VBC81_000355"
     CDS             complement(12653..13681)
                     /gene="queA"
                     /locus_tag="VBC81_000355"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6911420.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            13834..14514
                     /locus_tag="VBC81_000356"
     CDS             13834..14514
                     /locus_tag="VBC81_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6911421.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            14665..15366
                     /locus_tag="VBC81_000357"
     CDS             14665..15366
                     /locus_tag="VBC81_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6911422.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(15438..16394)
                     /locus_tag="VBC81_000358"
     CDS             complement(15438..16394)
                     /locus_tag="VBC81_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6911423.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            16490..17209
                     /locus_tag="VBC81_000359"
     CDS             16490..17209
                     /locus_tag="VBC81_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6911424.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            17282..18433
                     /locus_tag="VBC81_000360"
     CDS             17282..18433
                     /locus_tag="VBC81_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6911425.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            18485..19432
                     /locus_tag="VBC81_000361"
     CDS             18485..19432
                     /locus_tag="VBC81_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6911426.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            19448..21253
                     /gene="pepF"
                     /locus_tag="VBC81_000362"
     CDS             19448..21253
                     /gene="pepF"
                     /locus_tag="VBC81_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6911427.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVTKGVHND"
     gene            21448..22074
                     /locus_tag="VBC81_000363"
     CDS             21448..22074
                     /locus_tag="VBC81_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6911428.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFSLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            22148..22855
                     /locus_tag="VBC81_000364"
     CDS             22148..22855
                     /locus_tag="VBC81_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6911429.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            22916..23845
                     /gene="prsA"
                     /locus_tag="VBC81_000365"
     CDS             22916..23845
                     /gene="prsA"
                     /locus_tag="VBC81_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6911430.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            24087..24572
                     /locus_tag="VBC81_000366"
     CDS             24087..24572
                     /locus_tag="VBC81_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6911431.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            24588..27206
                     /gene="alaS"
                     /locus_tag="VBC81_000367"
     CDS             24588..27206
                     /gene="alaS"
                     /locus_tag="VBC81_000367"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6911432.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            27288..28004
                     /locus_tag="VBC81_000368"
     CDS             27288..28004
                     /locus_tag="VBC81_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6911433.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            28092..28910
                     /locus_tag="VBC81_000369"
     CDS             28092..28910
                     /locus_tag="VBC81_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6911434.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            29111..29299
                     /locus_tag="VBC81_000370"
     CDS             29111..29299
                     /locus_tag="VBC81_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911435.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            29331..29624
                     /locus_tag="VBC81_000371"
     CDS             29331..29624
                     /locus_tag="VBC81_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911436.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            29669..29884
                     /locus_tag="VBC81_000372"
     CDS             29669..29884
                     /locus_tag="VBC81_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911437.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            29887..>30198
                     /locus_tag="VBC81_000373"
     CDS             29887..>30198
                     /locus_tag="VBC81_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6911438.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SY"
BASE COUNT         9965 a         4659 c         5876 g         9698 t
ORIGIN      
        1 gctcgtcatg ctaaaaatga ttagttgtta ttctataata aagtagatac gaaaacaaga
       61 aaataatgct ataaaatttc aacctattat aattttttgc tgatagtacg tatgaacctt
      121 taatttgcta aaatagaaga gaggagagtg ttattatgtt tcacagagaa gatttttcaa
      181 aagcattatt aaaaaaagct ttgcagcccg gtatgagagt tatggatatt ggttgtggta
      241 gtggggaatt aacgcgtttg gctgctgata tcgttggtaa agaaggtgat gttgtaggaa
      301 ttgatattaa tgagcaactt ttatggcaag ctagacatga taatggctat gctaatgtta
      361 gttatcaaaa tgcagattta gcagcacttt cctcctttga aacaaaaagt tttgatgcta
      421 ttattggacg tcgtgtatta atgtatcaaa aaaatccaaa gaaagtaata gaaagtttaa
      481 cacctttgtt aaagcaaaat ggtttttatg tttttcaaga aagtgatgca atgtgtttag
      541 aactagctaa gcctagcttt gtaaatatga ataaaataca atcactaatc tggcaaactg
      601 ttgagaagga aggaggacac attcatattg gaagtcaact tgagcaatta atgttagaag
      661 caggcttaat cgttgaccat tttgtatcag aaaatgtcat tcaaacgtca caaactggta
      721 cagatctatc ttggctacta aatatcatga aagaaagaat acaaaaatat agtgaccttc
      781 aggggttaga agatgtcaat ttacaaaaag aattagaaga gaataatgac cattttatcc
      841 gagattgtca atttggaatt atttctcata gagttgatga atgattttaa gtattatttg
      901 acaccttgaa aagtttcctt atggtataat aaggaatatg attgcgattg aagaaattgg
      961 gagaattacc ccagataatt tagggttagt aactgtttta gcaggagaag atttagggca
     1021 atatgctcag atgaaagaga agctatttca ggtaattgga ttcaataaag acgatttagc
     1081 ctattcttat tttgatttaa gtgaagaaga atatcaaaat gcggaattag acttagaaag
     1141 tttacctttt ttatcagact acaaggttgt tatttttgat cagtttcaag atattacaac
     1201 tgataaaaaa acttatttag atgaacaagc aatgaagcgc ttggaagcct acttgcagaa
     1261 tcctgtagat actacgagac ttgttatttg tgctccagga aaacttgatg gaaaacgtcg
     1321 acttgtaaag ttattgaaac gtgatgctcg tgttttagag gctaatactt taaaagaatc
     1381 agatttaaag acgtattttc aaaaatatgc tcaccaagaa ggattagttt ttgaagcagg
     1441 tgtttttgat gagttattga ttaagtctaa ctatgatttt agtgatactt taactaatat
     1501 cgctttctta aagtcatata aaacagacgg tcatatttcg tctaatgatg ttagagaggc
     1561 tatccctaaa agcctgcaag ataatatatt tgatttgact caagatgtct tacttggtag
     1621 gatagattta gcgcgtgatt tagtaagaga tttacgtcta caaggcgaag acgaaatcaa
     1681 attgattgca atcatgttag gacagtttcg gatgtttttg caagttaaaa tattggctag
     1741 taaaggaaag tcagagagtc aaattgtttc tgaactatcc cattacatag gacgtaaaat
     1801 caacccttat caagtaaaat ttgctgtcag agattctaga aaccttcccc ttgctttttt
     1861 aaaagaagcg attaggattt taatcgagac tgactatgct attaaaagag ggacttatga
     1921 taaggattat ctctttgatt tagccttatt aaaaattgct cataaaaagc cctaattaaa
     1981 gaaacaaaag agttgaaagc tgttccaaat tcctatagaa taagggtaac aatagatgaa
     2041 aagaggattt cgacatggca attattttac cagatttacc ctatgcatat gatgcgcttg
     2101 agccacatat tgatgctgag acaatgacac tacatcatga taagcaccat gcaacttatg
     2161 ttgctaatgc aaatgctgct cttgagaaac atcctgaaat tggagaagac ttagaggcgc
     2221 tcttagctga tgtttctcaa attccagaag atattcgtca ggcagtcatc aataacggtg
     2281 gtggacatct taaccacgct cttttctggg aattgatgtc accagaagaa actcaaattt
     2341 cacaagagtt atctgaagac attgatgcaa cttttggttc atttgaagac tttaaagctg
     2401 ctttcacagc agcagcaaca ggacgttttg gttcaggttg ggcttggctt gttgttaatg
     2461 ctgaaggcaa acttgaagtg ctttcaactg ccaatcaaga tactccaatt atggaaggta
     2521 agaaacctat tttaggactt gatgtatggg agcatgctta ttacttaaac tatcgtaatg
     2581 ttcgtcctaa ctacatcaaa gctttctttg aaattattaa ctggaataaa gtaaatgaac
     2641 tttaccaagc tgctaaagct taataaaaaa acgagtcgat aggctcgttt ttttattatt
     2701 ctaaaagttg ataatgtttt taaaagtcta gaaatccaaa ctcagataga actagaacag
     2761 tcctattatc ttcaattaag cgaataagtt tattgaaagc gcttgacaag attagtaaga
     2821 aatgataaaa ttaagacgtt ttcaggtgtt attatttaag gtaagcaagc tgataacaga
     2881 taaaattgca taataaagga tgttatcaca ttttgtgagc agggacctat tcaacgacga
     2941 ctttttgttg tgtcttggat gggtcatttt ttattgtgcg agtgaaggac taggacttta
     3001 atgattataa aacgcgtatt aaatcataat gcagtcatat cagtaacaca tcaaggatta
     3061 gatgtgttac tgatggggaa aggaattgca tttaagaaaa gaataggtga taggattaat
     3121 agcgatgcta ttgagaagtc atttgtttta aaaaatagtg ataacatgaa ccgttttacg
     3181 gagttattta ttactgttcc agaagaagta gttgcatgtt cagaacgaat tatcaaccta
     3241 ggtaaaatca aattgggtaa gaacttagat gaaattcttt atatcaattt gacagatcat
     3301 attcattcgg ctattgaaag acatgaacaa agaatggtta tccaaaatcc actaaggttg
     3361 gaaatccagc gttactatcc tgatgaatac tccattggga tgaaggctct ggaacttata
     3421 aaggatgaat tgggtatttg tttaacaatt gatgagtcag cttttattgc tatgcacttt
     3481 gtaaatgctg gattggataa tccgtttaat gaagcgcata aaatcactga gattgtatct
     3541 tacattgaac agaaagtaaa aatagatttt cgaacagaac tagatgaaag ttcaattgat
     3601 tattatcgtt tcatgacaca taccaaatta tttgctcaac gcgttttatc aggaatgaaa
     3661 tacgaagatg atgatgctga tttacttttg gtagttaaga aaaaatatcc gagagaatat
     3721 aaatgtgtta aagagatcgg caacaatatg gctatccaat accaatatca acttaattca
     3781 agtgaactcc tatatctgac ggtgcatgtt aaacgattag ttaaaaattt aaaggagata
     3841 tcagatgact aaataccaag agactgctaa agctatttta gcagcagtag ggggagaaaa
     3901 aaatattcaa cacgtaaccc actgtgtgac acgattacga ttagttttag acaatgatga
     3961 aattgtcaac gatcaggtta ttaaaactat tccgaatgtt atcggtgtta tgcgtaaaaa
     4021 tgatcaatac caaattattt tgggaaatga tgtcaataat tattataatg cttttttggc
     4081 cttaggtcat tttgaaaata ctaccagaga attttcatca caaaaaaaga gttcaatatt
     4141 tgagaaatta attgaaacga ttgcaggagt cattaagccg cttatacctg ctcttctagg
     4201 aggaggtatg ctcaaggtta taggtatttt gctccctatg ctcggtataa caagctctag
     4261 ttctcaaaca gtagctttta tcaatttttt tggtgatgcc gcttattatt ttatgccaat
     4321 aatgattgct tattcagctg cgtcacgatt taaagttaca cctgtattag ctgctacagt
     4381 aggagggatt ctcttacacc ctgcttttgt gacaatggta gctgaaggaa aaccattatc
     4441 tttctttgga gctccggtta cactggctag ttatggttct tctgttattc caatcttaat
     4501 tatggttttt cttatgcaat acattgagag atggattaat aaaattgttc ctagtgtaat
     4561 gaagagcttt ttacaaccga ctttgattat tctcatttct ggctttttag ccttagtggt
     4621 cgtggggcca ctgggagtaa ttattggtaa aggattgtct agtgcaatgc tttcgattta
     4681 ccatgtggca ccatggttag ctttagctat tcttggtgct attatgccat tggttgttat
     4741 gacaggaatg cactgggcat ttgctccaat ctttttagct gcttctgtta cgacaccaga
     4801 tgttttgatt ttaccagcaa tgttagcttc taatttagct caaggtgcag cttcccttgc
     4861 tgttgcagtt aaggctaaac aaaaacagac acgtcaggtg gcctttgcag caggtttatc
     4921 agcactacta gctggtatca cagaacctgc actttatggt gtcacactga aattcaagaa
     4981 gcctttatac gctgcaatga tatcaggtgg tttagtagga gcttatattg gattagttaa
     5041 tattgcttcc tatacatttg tagtgccatc tattattggt ttgccacaat acatcaatcc
     5101 acaggggggc aataatttca gtaatgctgt tattgtagca atagctacta ttattttgac
     5161 tttcattatt acatggttct tgggaattga tgaaggagaa aacgaaaaaa gtagtattaa
     5221 tgctcaagag tacacacata ttagaagtgg tttatcaaaa aaagagacat tgtattcacc
     5281 tatggtaggc aatgtattac ctttatctaa agtacccgat gaaacatttt catctaaatt
     5341 actaggtgaa ggtttagcaa tcactcctag tgtgggtgaa gtttatgcac catttgatgg
     5401 tgaaattata agtttatttc ctacaaaaca tgccattgcc ttaaaggatg ataagggagt
     5461 tgaagtgtta atccatattg gaatagatac tgtagaacta aatggagaag gttttgaaca
     5521 attggttaaa gttggcgatt ttgttaaacg aggacagtta cttttgcgaa tggatattga
     5581 ttttattagt tcaaaggggt attctcttat tagtccagtt gtagttacaa attcaattga
     5641 ccaacttgaa attatcgtta aagacgaaga gacaatggtt acaaatgaag atgatttact
     5701 tgtaattctt taaaaggaag gatgataata tgacgatttt tcctaaacat tttctatggg
     5761 gaggtgcagt tgctgccaat caggtagagg gtgcatttag aacagatggc aaaggtttat
     5821 ctgtacaaga tgtacttcca aatggtggtc taggcgattt taccgccaaa cctactccag
     5881 acaatttgaa gctagaagca attgattttt atcataacta taaaaatgat ataaaactgt
     5941 ttgcagagat gggctttaaa gtttttagaa cctctattgc ctggtctcga atttttccaa
     6001 atggtgatga tagtgctcca aacgaagctg gcttacaatt ttatgataat ttatttgacg
     6061 agttgcttaa atataatatc gagcctttgg ttaccttatc tcattatgaa acaccgcttc
     6121 atcttgcaaa gacctataat ggttgggctg atagacgttt gatagcattt tttgagaaat
     6181 ttgctcaaac agtcatggag cgttataaag ataaggtaaa atattggtta acttttaatg
     6241 aagtgaattc tattttacat atgcctttca ccagtggtgc tattatggcc gacaaatcac
     6301 aactaagtcc tcaggagctt tatcaagcta ttcatcatga attagtagca tcggctagag
     6361 tgacaaaatt agggcgttct atcaacccta attttaaaat tggctgtatg attctggcta
     6421 tgccagctta tcctatgaca tctgatccaa gagatgttct ggctgcaaga caatttgaac
     6481 aacataatct gctattttca gatatacatg tcagaggtaa atatcccact tatattcagt
     6541 cctatttcaa aaataatggt attgaaatta aatttgaaga aggagatgag gaagtattgg
     6601 cacaaaatac agtagatttc ctatcgttta gttactatat gagcgtgaca caagcttatg
     6661 actttgaaaa ttatcaaagt ggtcagggaa atatccttgg aggcctgact aatccttatt
     6721 taacaacttc agaatgggta tggcaaatcg atcccatagg gctacgccta gtcttaaatc
     6781 agtattatga acgttatcag attccgttgt ttattgtaga aaatggattg ggagcaaaag
     6841 atcaattgat agaaacacta gacggtgatt atactgtcga agatgattat cgtattgatt
     6901 atatgaatca acatttagtt caagttgcta aagctattga agatggtgtt gaaataatgg
     6961 gatatacatc ttggggatgc attgattgtg tatccatgtc tacagctcaa ctaagtaaac
     7021 gttatggtct aatatatgtt gatcgaaatg atgatggaac agggagtcta caacgttaca
     7081 agaagaagtc atttggttgg taccaaaaag tgattaagac aaatggccaa tcattgtttg
     7141 aacatcataa cagataacat gttaaacaac tagtctctgt ttcaatttaa tataaaaagg
     7201 agtatccata ggttactcct ttttattata atttatgaag gtaaatatct tctgatttaa
     7261 gtgctagata caataaagtt gcatccttaa agtttcttgg attgagttgc gttgattcat
     7321 aaatcttatt taacctatat tgaacagtat ttttatggat aaaaagttcc tgactacatt
     7381 cttttaatga tagattatgc ttaaaatatg agttaagcaa gatcttatca tttttagata
     7441 agtttacaag cgctttattt agcacatatt gtttaatatt agaatcaata cttgttaata
     7501 acagttcaag atcaaggtcg tcgacgaggt tgaccttttg ttgacctttt aaagctttca
     7561 gggctaagat agacgtttca tatgatcttt ttaaaagtga taaagatgat ttttgtccga
     7621 taccaacttt atagagaccc ttttcgcatt caaactttga taggatattt ggataataat
     7681 agtcaaacat ctggtcagaa agtaaaagcc agtattgatt gggataatta aaggtataaa
     7741 tagcagtgtc aagtttatca aaaaagttca agagtgacat ttcagattga cttaagagta
     7801 atttttgttt ttgactattg atttcaaaag tgagtagacg atagtcagtt ttttctgata
     7861 ggtggtattg gtttaaaaat tgattgaggt aatagtagtc taactctctg ccttcaacaa
     7921 gatggtgtaa gacaatagaa gcttcgtttt ttcgcccgaa atccagataa tctaattcat
     7981 gctcttttaa gatgagtcga gtcatttttt gagcaagtaa agcatatttt cctacttggt
     8041 tggggtttcc actaatccca atagtagcta ataattcaca attataatag aaaggaatat
     8101 tgatccctgc ttgtgtccca aaataagatt cttgatctgt tacttcaatc atttgtcctg
     8161 tctgtgctac ttttaaacca atttcatgga attctccaac acgttttgga ttggtactag
     8221 cgaaaatgat tcctttggta ttaataaaat taatatcttg ttgacaaaca tctttgatag
     8281 aggtaacgat ttgttgtgcg agttgttttc ttaatttcat tcagcttacc ttttgttata
     8341 tataacaaat atcatatcat aaaaacgcta acatttgttc tatagtctat attttttgtt
     8401 atttgatttt gttactataa ttgagtgata taaaatagtg aaaaaataac aaagattcac
     8461 aaaatatttt caaggaggtt ttcaaatgaa ggtagtagta gctattgatt ctttgaaagg
     8521 gagtctttct tctttagaag ctggcaatgc tataaaggag tcaattaatg aagtaatccc
     8581 aggggcggat gtggaagtac atccattagc agacggggga gaaggaactg ttgaagcttt
     8641 gacgctaggt atggggggga cgatcgaaat aatccctgtt aaggggcctc ttggagaaaa
     8701 agtacatgcg agttatggta ttattccaca gcgtcagtta gctattattg aaatggctgc
     8761 agctgctggt attaccctaa ttgctactga agagagaaat cctctccaca caaccactta
     8821 tggagttgga gaaatgataa atgatgctat aagtaaaggc tgtaggcact ttattattgg
     8881 tattggaggt agtgctacta atgatggtgg ggcaggtatg ctacaagctt tgggatatgc
     8941 attattagat aaagataatc aagaaatcag tttgggagca caaggattgg cagatttaaa
     9001 atctatctct actgataagg ttattgagga attaaaagaa tgcgatttta aaattgcatg
     9061 tgatgtaaca aatcctttat gtggtgctca aggttgtagt tcgatttttg gaccgcaaaa
     9121 aggtgctgat gaagatatga ttacaaaaat ggatacttgg ttaagtaatt atgctacttt
     9181 agctacttca gttagtgaaa aagcagatgc aactattgaa ggaacaggtg cagcgggtgg
     9241 gcttggattt gcctttttag cctttacaaa tgccacctta gaacctggta ttgatattat
     9301 tctatcagaa attaacatag aaaaagcgat ttctgaagct gatttagtag ttactggtga
     9361 aggtcgtttg gatgggcaaa cggtgatggg aaaagctcca attggcgtag caaaattagc
     9421 taaaaaatat ggtaagaaag tagtggcatt ctctggatct gtcaccgaag atgctatact
     9481 ttgtaatcaa catggtattg atgctttttt tccaatcgta cgacgcctta tcagcttaga
     9541 tgaggctatg tctaaagagg tagcttataa aaatatgaaa gaaactgcta ctcaagtctt
     9601 tagattaatc aatttgtata atcattaata ttgaaaggaa ttctgataaa ttatggaagc
     9661 tacatttaca acaacagggg ctttaatagg attagcgctt gctattttac tgattatcaa
     9721 aaaagttcat cctgcctata gtctcattct tggagcatta gttggtggtt tgattggtgg
     9781 cggtgattta gttacaattg ttaatactat ggtattaggt gcgcaaggga tgatgtcatc
     9841 catattacgc attttaacgt cagggatttt agcgggtgct ttaattaaaa ccggatcagc
     9901 tgaaaaaatt gcagaaagta tcattaaaaa attaggacaa cagcgagcca tcacagcttt
     9961 agccatcgca acaatgatta tttgtgcagt aggtgtcttt attgatattg cagttattac
    10021 agttgctccg attgcattag caataggtaa aaaagctaat ctttctaaat ctagcatact
    10081 gttagcaatg attggtggtg gtaaggctgg gaatattatt tcacccaatc ctaatacaat
    10141 tgctgcttca gaagcattta aggttgattt aacaagctta atggtacaaa atattattcc
    10201 tgcaattgca gctttagtag ttacgattat attagcaaag attgtttcca agaaaaataa
    10261 cgatatttct tatgatagtg aggagcaagt gggctctgat ctgccagcat tcttaccggc
    10321 tatttctggt ccacttgtgg ttatctgctt attagctctt cgtcctttat ttggaatcac
    10381 tattgatccc ttgattgcac tgccgttggg tggtctaatt agtattttag caacaggtta
    10441 cctaaaagaa acggtaccgt ttgttgaata cggtttatca aaagttgttg gcgtttcaat
    10501 tcttttgatt ggaacaggta cactttcagg tattattaaa gcatctaatc tacaatttga
    10561 tatgattcat ctccttgaat ttttaaatat gccgaccttt atccttgccc cactctcagg
    10621 tatttttatg ggagcagcaa cagcatcaac gacttctgga acaactattg cttcacaaac
    10681 gtttgctgaa accttgatta aatcaggggt accggcggtt tcgggtgcag ctatgattca
    10741 cgcaggtgcg actgttctgg attcgctacc tcatggatcc ttcttccatg cgacaggcgg
    10801 agctgtcaat atggctataa aagaacgtat gaaactaatt agctatgaag ctttgattgg
    10861 attaactagc acaatagttg ctgtcgttta ctattgtttc tttgggtaaa atttggatga
    10921 atacgtgatg tttattatca ctgtttgaat agaattctca ttgatttttt ctaattttct
    10981 tactataata aattagtagg agaggtgttg tgaaacaaac aaagaaagaa tttattttag
    11041 gattaaccat tattattacc cttactttat taatcatgct gattttttta gtaatgttta
    11101 ataagtccag taactctaag ttgaagcaga caaaagatag aagaatatta tctacttcaa
    11161 ccacaaggac atccagtagc tcacatgatc aaaaaattgt agaaaagtct cttgaaaaaa
    11221 agaaaatggt catggaacaa gatatggaat tgatgaatgc ctataggctc tattatgatt
    11281 atgcccattt gtccttgaaa gaggttgttc tggcctatat gtctgaatat ggcattgaaa
    11341 aaaatagtgt tgctttttct tataaaaatt taaaaacagg acaaagggac gctatgaatg
    11401 atactcaggc tatgacagca gggagcacct ataagttacc tcttaacatg ttggtagtag
    11461 atgcagtggt caagggcaag ttgtccatgg aagaacgctt tgatattacc gagactgact
    11521 atgaatatat tggtgaacat aataattatg ttgctgcttt ctcgggggca atgtctattg
    11581 atgatatgca aaaatactct ttagtttatt cagaaaatac acccgcctat gctttagctg
    11641 agcgtattgg tggcatggat agtgcttatt caaagtttgg tcgttatggc caatctaaag
    11701 gagacatcaa aaatatacaa aaaaacggta ataaagtgac aacagattac tatattcaag
    11761 tgctagacta tctctggaaa catcgtaaaa agtataatag tttaattact tatttagaag
    11821 aagcttttcc aacggattat tatcgtgctt tgataccatc tgatgttgtt gttgctcaaa
    11881 agccagggta tgtccgtgag gcgttgaatg ttggagcaat tgtcaaagaa gaagtgcctt
    11941 atatcgttgc tatttatact gcaggtttag gtggctctac tcaggaagat tcagaaatta
    12001 atggagtggg actatatcag ctagaacaat tatgttttgt tattaatcaa tggcatcgtg
    12061 ttaatatgaa ttagaaataa atctatcaaa ataataaaaa cctcttagat taactatcta
    12121 agaggttttt ataaatgtga ttttataact tgattaagtg aagaaagggc agaaacagtt
    12181 ttttgataag attcaggact aatttctgag aaaaaatacc cgcacgattg caccgcattg
    12241 acaacggcat cttgtgactc aatacctttg ttacttagat gaataatgag ttgccttttg
    12301 tcatctcttg gtttttctcg tacaatccac ccacgatttt ccatacgctt taaaactgga
    12361 gttagggtat tcgaattaag agatagtgca taactaagtt ccttcatatg ccgatgatcc
    12421 tcttcccaaa ggactaacaa gataacgtat tggagataag tcaattgaaa ttcatccaaa
    12481 gctttttggt aaaatttatt gaaaagtcga tgtgtttcac taaccgtaaa acataatcgc
    12541 tcgtctaaaa tacttcgatt actcattatc atattgtagc attttggagg cgttatagta
    12601 tgtcaatcgt gttaaaaaac gataaaactt tttcctaatt gaaagcctat tcttacttaa
    12661 caaacatagc atcaccaaaa ctgaagaaac gatatcgttc ttcaactgca tggttgtaag
    12721 cttctagtac aaagtcatgt ccagcaaatg ctgatactaa catgacaaga gtagactttg
    12781 gtaagtgaaa gttggttgaa aaagcgtcta caaccttgaa ctgataacca ggcttgataa
    12841 aaatatttgt ccaccctgag tcggctttta attcaccatt gaatttagaa ccaattgtct
    12901 ctaatgtgcg aatagaagtt gtaccaacag ctacaatacg tccaccagat tctttaactg
    12961 cattgagagt atcagcagct tctttagata attggtaaaa ctcagaatgc atttcgtgtt
    13021 catcaagatt atcaactgaa acaggacgga aagttccaag tccaacgtga agcgtcaaat
    13081 agacaagctt cacacctttt gtttcaattt tttccaatag ttcttttgta aaatgaagac
    13141 cggctgttgg tgcagcagca gaaccatttt cttttgcata cacagtctga tagcggtcac
    13201 gatcttctag tttttcatga atatatggcg gaagtggcat ttcgcccaaa ctttcaagga
    13261 cttctagaaa aataccatca tatgagaatt ctacaatacg cccaccatgt tctaattcct
    13321 ttgtgactgt tgctattaac cgaccatcac caaatgaaac tctgttccct acgcgtaagc
    13381 gtttagcggg tttagcaagc acttcccatt gatcgccctc tgtatttttg agaagtaata
    13441 actctacatg gccgtgtgta tctggctttt caccataaag gcgagcgggc agaacacgtg
    13501 tgttattcat tactaaagcg tcaccaggct tgagttcatc taagatatga tcgaaatggc
    13561 tatctgtcat cgtcttattc ttatgatcaa tgactaatag tttagaagcg tctctttttt
    13621 caagtggtgt ttgggcaata agttcttctg gtaaataaaa atcaaaatcg tttgtattca
    13681 tgatttctcc tatctataag taagcttttc tattatatca cgttcggtaa aaatgaccca
    13741 actaatcttc ttatttagaa tgattataaa aaataattgt aatcattcta aataagtggt
    13801 ataataattg tcagaaagtg agaagtagag aagatgaaag aagcaaaaaa tttttcccaa
    13861 caattgaata ttttaagagc tggtgttctt ggtgctaatg atggtatcat ctctgtagct
    13921 ggtgttgtta ttggtgttgc gagtgcaact cataaccttt ggattatttt tttatcagca
    13981 gcttccgcga ttttagctgg tgcattttct atggctggcg gggagtatgt tagtgtttct
    14041 actcaaaaag atactgaaca agcagcagtt gcgcgtgaag agaaactact agaaaataat
    14101 ccagaacttg ccaaaaaatc cttagttgat atctacttag caaaaggaga aagtcacgaa
    14161 catgctcaat ggctagttga caaggctttt tcaaagaatg ctattgagca tttagttgaa
    14221 gaaaaatatg gtattgagtt cggtgaatat acaagcccct ggcatgctgc tatatctagt
    14281 tttatagctt ttgccatcgg atcaattttt ccaactataa ctattctgtt attaccattt
    14341 tcggtacgaa ttgtaggaac ggtaataatt gttattgttt cactattatc aactggttat
    14401 gtaagtgcta agttaggcca agcccctaca gtacctgcca tgcgtcgaaa tgtgatgatt
    14461 ggttgtttga caatgttggc aacctatgtc attggacaat tattttcaat ataaaatatt
    14521 aatcgtggag ttattgtcta aactagaatg gacagtaatt ctttttagtt ttataacgaa
    14581 attaagaagt ttttattgac aaaaattggt atataccata taatataatc ataaaggtct
    14641 ataccaaata aaaggagagt aaacatgaga gttattacag ttaagaacga cattgagggg
    14701 ggaaaaattg catttactct tttagaagag aaaatgaaaa ctggtgctca aactttagga
    14761 ttggcaacag gaagtagccc aatcaccttt tacgaagaga ttgtaaaaag taaccttgat
    14821 ttttcaaata tggtttctat taaccttgat gaatatgttg gaattgccgc aagtaatgat
    14881 caaagttact cttattttat gcataaacat ctttttgatg ctaaaccttt taaggaaaat
    14941 aaccttccaa acggcttggc taaagatttg aaaaaagaaa taaaacgata cgatgctgtc
    15001 ataaatgcta atccaattga ttttcaaatt cttggaattg gtcgtaacgg tcatattggt
    15061 tttaatgaac caggtacacc ttttgatatt acaacacatg tggttgactt agctccaagt
    15121 acaattgaag ctaatagtcg tttctttaat agtattgatg atgtaccaaa gcaagcctta
    15181 tcaatgggta taggttctat tatgaaatca aaaacaatcg tattagtggc gtatggtatt
    15241 gaaaaagcag aagcgattgc aagtatgatt aaaggaccta tcacagaaga tatgccagca
    15301 agtatcctcc aaaaacatga tgatgttgtg attattgtgg atgaagcagc ggcatcaaag
    15361 ttgtaaagta aataataaaa aagtaaggat gaatctcaat gagaaaaaat tcattcttac
    15421 ttttttatta tgctttatta aattgtccag tctaagttag gtccagctgg aataataccg
    15481 gtagggttaa tggttttatg gctaccgtaa tagtgttttt tgatatgatc aaaattaatt
    15541 gtctcagcaa ttccaggtag gttgtaaata cgttttgtat aatgccataa atgtgggtaa
    15601 tcatgtagcg cttttaaatt acatttaaaa tgaccataat aaacggtgtc aaagcgaact
    15661 aaagttgtga aaaggcgaat atcagcttcg gtaaattgtt ctccaacaag ataatggtta
    15721 tcagataagt gtttttctaa ctgatctaaa gctgtgaata aggtttcaac ttccttttga
    15781 taaacgttct gagaagtagc aaaaccagct ttatagacac cattatttat ttttggataa
    15841 ataaaattat tcatttcgtc aatttgtcct tgtaaggaat cgggatagta atcgtcagta
    15901 tttccagtaa tatgattaaa tgcagtattt aacatgcgca taatttcaga tgattcattg
    15961 ttaacgattg tatggcattt tttatcccaa agaacaggga ctgtcacgcg tcctgtataa
    16021 tcactttgac ttgcttgata gatttggtaa aggtattggc tttggttaat catatcagga
    16081 ataacgcctt tatattcttg aaaagtccaa ccattctcca acattaaagg gttaacaatg
    16141 gagatagaga tatgagattc tagattttta agctttctca tgattaatac acgtgatgcc
    16201 caagggcagg ctagtgaaac gtagagatga tatcgtccag attctgcttt aaaacctgca
    16261 tccccactag gccctgctga accatcttta gtaacccaat gacggaattg tgtaacggtt
    16321 cggacgaatt taccaccagt tgatgcagta tcataccatt gatcaaccca tttgccatct
    16381 actaaaagtc ccatgataat ctcctttgtt tttctattac taattagtag tataaagatt
    16441 ttaaaaggaa ttagcaaatg tttaacttac agtatggtaa actaagggta tgcgtttaga
    16501 taaattatta ggtcaggcag gatttgggtc tagaaatcag gttaagaaat taattcgttc
    16561 taggcaagtt tctgtagatg gtcaaattgt tacaaaggat aatgttattg tagatagtgg
    16621 tctacaatcg atttttgtag gaaaagagag agtctgccta aaagaaagtt cttattacct
    16681 tctttataag ccatcaggag ttgtttcagc agttagagat tcagaacata aaacagtcat
    16741 agacttaatt agcgaaaaag ataaggtgga gggattgtat ccgattggtc gtctagatcg
    16801 tgatacggaa gggcttttaa taatcacgaa taatggtcca ctgggttacc gaatgttaca
    16861 tccaaaacac cacgttgcaa aaacgtatta tgttgaagta aatggtttct tggaaagaga
    16921 tgctattacc ttttttgaag aaggagttgt atttgacgat ggaacaaagt gtaaaccagc
    16981 tgaattaacg attgatacgg ctaataatga caaaagtacc gcaagaataa ctatcacaga
    17041 agggaaattt caccaagtaa aaaagatgtt tcttgcatat ggcgttaaag ttatatactt
    17101 gaggcgtatt tcttttggtg acttgaggct tgatatgaat ttgaaaccag gtcagtatcg
    17161 tagattgaga gattcagagg cagctatctt aaaaaggtat ttagattagt gaaacgactc
    17221 acttgacctt gcttagtatt cttcatatac ttataagtga ttattttaaa ggagaaaact
    17281 attgaattca aaactttcat tgaaattgac tattttatcg atttctatct ttttgatgtc
    17341 tcacttagca attgctcctg caattcctaa attgtatgcc ttttaccata ctcaaaaccc
    17401 tcatattgga ctagcttcag tagagagttt agttactatt ccagctatga tgattactat
    17461 ttttgttatt ttgagtaatt ttgtagtcac aaaacttggc aaaaaaaaca cggtcttact
    17521 tggtttatgt ttaatcttaa tgtctgggtt tatctcattt tttacaagta atttttcact
    17581 tgcgatggct agtcgactac ttttgggtat tggtattggt ctctataatt cgctatcaat
    17641 tagtatcata acggatttat atgaggctga tgagagagcc agtatgattg gtcttagaac
    17701 agcatcactt aatatcggta aagccttaac tacatttatt gttggattgg ttttagctat
    17761 tggtgtgaat tatatttatt tggtttacct tctggttatt cctgttttct ttttcttttg
    17821 gaaaaatgtt ccagaggttg aaaatcaaac acataccctt aaagctagta caacatttga
    17881 tactaaagcg gctttgttga tgctcatcac gttcttagta ggtattgctt atataggagc
    17941 aactgtcaaa attccaacct tactagtgac aaaatatcac tacgcgacga gcttctctag
    18001 taatatgtta actctccttg cttttagtgg tattttagtt ggatctgtat ttggaaaatt
    18061 agtcaaagca ttccaagaga agacgctatt aatcatgatt ctagctatgg gaataggtaa
    18121 tgtgctattt gctttagcta ataatcagat catttttatc gtagctagta ttttaatagg
    18181 agctagtttt gttggcacca tgtcttctgt attcttttat atttcaaaaa actatgcaaa
    18241 agaacataat aactttatca caagccttgc cttaacagct ggtaatatag gagttatttt
    18301 gacaccgctt attttaacga aacttccaag ccagctacat ctggaacctt ttataacacc
    18361 atttttaata acaagtgggc tgatggtgat taatgtcttt gtttacctag ttttaatgtc
    18421 aaaaaataaa taaaaagtta tccgaaagga tagctttttt cgaatagtaa aagtaggaga
    18481 aaaaatgtta attgctaaag acaaacaagg aaatttaatt aatctactcg agtctcatcc
    18541 gggaaaggga cagtattttt gtccaacttg ttgttcagct gtaaggttaa aagcaggtcg
    18601 aatcatgaga agacactttg cacatattag tctaaaaaat tgtcagtttc atcatgaaaa
    18661 tgagtctaat gagcacttgc aattaaaagc aaagttatat atgagtttaa gtagagagaa
    18721 tgaaactatg ctcgagcatc atttgccaga aattaatcag attgcagatt tatttgtgaa
    18781 tgagacattg gctttagaag ttcagtgtag ccgactatca gagcagcgac tcagggagcg
    18841 aacaaaagca tatctacagg cagactttca agtgagatgg ttactagggg aaaaactctg
    18901 gttaaagcat cgtttaacaa atctacataa acaattttta caattttctc aaagtatcgg
    18961 atttcatatt tgggagttag atttgaggtt agaagtactg agactaaaat atttgattta
    19021 cgaagattta cgcggacatg tctattactt aagtaaaact tgccctctta gtggagacgt
    19081 tctagcattt ttgaagtggc cataccaatc aaaaaatcta aatttttata aggttaaaca
    19141 agacagaaat atcagagatt atgtgcgtca gcagttacgc tatggcaatc aattttggtt
    19201 acgaaaacaa gaaaaggcct acctttctgg tcagaatcta ttaacccaag agctaatgat
    19261 gttttttcct caaatacagc caccacgtgt agatactgat ttttgtcaaa taacaaattc
    19321 tctgacatcg ttttatcaga attttacaaa ttattatcaa aaaaataaga ataacttgga
    19381 ccaaacactt tatccccctg tattttatga taaaatagtc actaagaatt aaactggagg
    19441 atacaaaatg tcagacaatc gctcacatat tgaagaaaaa taccaatggg atttaactac
    19501 tgtctttgcg actgatgagt tatgggaaac tgaagtggta gagcttactc aagccatcga
    19561 tgacgcaaag ggtttttcag gtcatctgtt ggactcaagc caatctttat tggagattac
    19621 ggaggttgaa ctagagttat ctcgccgttt agaaaaagtt tatgtctatg cttcaatgaa
    19681 aaatgaccaa gacaccactg ttgcgaaata tcaagaattt caagcgaaag cgacagcgct
    19741 ctatgcaaaa tttagtgaaa cattttcgtt ttacgagcca gaactattgc aactttcaga
    19801 gtctgattac cagtcattcc ttttagaaat gcctgacttg caaaaatatg atcatttctt
    19861 tgagaaaata tttgcaaata aacctcatgt cttatctcaa aacgaagaag aattgttagc
    19921 tggtgctagt gagatttttg gagcagctgg tgagacattt gaaatccttg ataatgcaga
    19981 tatggtcttc ccagttgtaa aaaatgcaaa aggtgaggaa gttgagctta cacatggtaa
    20041 tttcatttcc cttatggaat ctagcgatag gacggttcga aaagaagcat atcaagctat
    20101 gtatagcaca tatgaacaat tccagcatac ttacgctaaa acattacaaa caaatgtgaa
    20161 atctcaaaat tttaaggctc gtgtgcatca ttatcaatca gcacgccaat cagctctatc
    20221 tgccaatttt attccagaag aggtctacga aactctaatt aagacagtta atcatcattt
    20281 accgctctta catcggtata tgaagttgcg tcaaaaagtt cttgggctag atgatttgaa
    20341 gatgtatgac gtatatacac ccctttctca aatggatatg agttttactt atgatgaagc
    20401 tctaaaaaaa tcagagaagg ttctggctat ttttggcgaa gcatattctg aacgtgttca
    20461 tcgtgctttt actgaacgtt ggattgatgt ccatgttaac aaaggaaaga ggtcaggcgc
    20521 ttattcaggt ggatcttatg acactaatgc tttcatgctt cttaactggc aagatacttt
    20581 agacaatctt tatacgctgg tgcatgaaac tggacatagt ctacactcga cgtttactcg
    20641 tgaaaatcaa ccttatgttt atggtgatta ttctattttc ctagctgaaa ttgcatcaac
    20701 aacaaatgaa aatatattaa cagaaaccct tttgaaagaa gttaaagatg ataaaaatcg
    20761 ctttgcaatt ctcaatcatt atttggacgg tttcaaagga acaattttta gacaaacaca
    20821 gttcgctgaa ttcgaacatg ccattcatgt agctgatcag gaggggcaag tactaactag
    20881 cgaatactta aacaaccttt acgctgagct taatgaaaaa tattatggtt tgactaaaga
    20941 agataatcat tttattcaat atgaatgggc aagaattcca cacttttact acaattatta
    21001 tgttttccaa tatgcaaccg gcttcgcagc agcaaattat ttagcagagc gtattgtaaa
    21061 cggtaatccg gaggataaag aggcctacct aaattacctt aaagctggga attcagatta
    21121 ccctctaaac gtcattgcta aagccggtgt tgatatgact agtgcagact atctggatgc
    21181 agcttttaga gtatttgaag agcgtttagt ggaactggaa aatcttgtaa caaaaggcgt
    21241 ccataatgat taagttgaaa atatagctat tgaagtaaaa ccagtcaact agactggttt
    21301 ttgatagtga tcagttattt tatagtttgt tttgagagtg gttttggtaa agttagttag
    21361 tggaaaactc aataggtgga aatacctacc caagctatga tttttatgag accaaaactg
    21421 ctattgtcaa aaaagaaggg aaataggatg aaaaagctta cttttatttg ggatttagat
    21481 gggacattaa tagattcgta tgtaccaatt atggaagctc ttgaagaaac ctatcgtcat
    21541 tttagcttaa tatttgataa agaattaatc catgaatata ttttacagga atcagtgggg
    21601 caattattgg taaacctttc agaggaagag caaatacctc atgaaaaact gaaagcatat
    21661 tttacaaaag aacaagaaag tcgagattct aaaatacatt taatgccata tgcaaaagag
    21721 attttagaat ggaccaaaga acaagatatt cccaatttta tgtatacaca taaaggagca
    21781 agtacgcatt cagtgttgga aaccttgcag atctctcatt attttgatga aattttaact
    21841 ggtgtttcgg gattcgagcg aaaaccacat ccacaaggga ttaattattt agttaaacga
    21901 tattctttag ataaatcaat gacttattac ataggagatc gtccactaga tttggaggtt
    21961 gctcaaaatg ctggtataaa atccataaac ttaaggttag agaattccaa agaaaactat
    22021 aatatttcaa gtctcaaaga tataatatca cttgatttca ctcgtttgga ttaaatcatt
    22081 ctcagagtta gacatttttt gctataataa aaaagttatt atttaaaata gaaaagagga
    22141 gccaaaagtg gttcaatctt acagtaaaaa tgctaatcat aatatgcgcc gtccagtagt
    22201 taaagaagaa attgttcaat atatgcgtca gcaccaaaaa caaaataatg gctgtttagc
    22261 agagttagaa gcatttgcta aacaggaaaa tatccccatt atacctcatg aaacagcgac
    22321 ttattttcgt tttttgatgc aaacattaca acctaagcac attttagaaa tcggaacagc
    22381 tattggtttt tcggcccttt tgatggcaga aaatgctcct gaagcaaaga taacaacaat
    22441 cgatcgtaat gaagagatga ttgccttggc taaggaaaac tttgcaaaat atgataatca
    22501 caatcagata acccttttgg aaggcgatgc tgtggatgtg ttacaaacat tggataaatc
    22561 ttacgatttt gtatttatgg attctgctaa gtctaaatat atcgtttttt tacctcaagt
    22621 attaaagcat ttagatgttg gaggagtagt tgttttagat gatattttcc aaggtggaga
    22681 tattgcaaaa cctatcgatg aagtgagaag gggtcagcga actatctacc gaggtctgca
    22741 acgtttattt gactcgactt tgcaacatcc tgatttaact gctactttgg tacctttggg
    22801 agatggttta ttgatgattc gtaaaaatgc agatcatatt gtactagaag attaaggcat
    22861 ttttaagaaa ttatgatata ataatggagt taatgacaaa aaggagttat aaatcatgaa
    22921 aacaagatca aaacttgcag caggcttttt gacacttatg tcagttgcca cattagctgc
    22981 ctgctcaggt aaaacatcaa atggaacaaa tgttgttaca atgaagggcg acactattac
    23041 agtctctgat ttttatgatc aagtaaaaac atcaaaagct gcacaacaat caatgcttac
    23101 attgatcctc tcacgtgttt ttgatacaca gtatggtgat aaagtttcag ataaaaaagt
    23161 atcagaagct tataataaga cagctaaagg ctatggtaat tcattttcaa gcgcactttc
    23221 acaagcaggt ttgactccgg aaggttacaa acaacaaatt cgcacaacta tgcttgtgga
    23281 atatgctgta aaagaagcag ctaagaaaga attaacagaa gcaaactata aagaagcata
    23341 taaaaactat actcctgaaa cttctgtaca agtaatcaaa ttggatgcag aagataaagc
    23401 taaatctgtc cttaaagatg taaaggctga tggagctgat tttgcaaaga ttgcaaaaga
    23461 aaaaacaaca gctactgata aaaaagttga gtataaattt gattctgcag ggacaagcct
    23521 ccctaaagaa gttatgtcag cagcctttaa gctagataaa aatggtgttt cagatgtggt
    23581 ttcaacggtt gattcaacaa cttataaaac aagttactac atcattaaag taactgataa
    23641 gacagagaaa aaatctgatt ggaaatctta caaaaatcgc ttaaaagaag ttattcttaa
    23701 ggataaaaca agcgatagat ccttccaaaa taaagtgatt tcaaaagcct tagaaaaagc
    23761 taacgttaag attaaagata aagcatttgc aggcatctta tcacaatatg ctacaacaag
    23821 tggttcatca tcacttaaaa aatagttgac caagtcttaa aaagactgta taattaatac
    23881 tattgagaat gaaatttcaa cgattagata cagaaaagtg acggttgctg cgagtcatgg
    23941 ataatcagaa aaagatgcta ctcaatgggg taaaactaaa aataaatgag aatgacaagt
    24001 tcattgaatg aaggtggtac cgcggttttc gcccttcgta atggacttgt cttttgctat
    24061 ttgtcaactt tcgaggagga agagatatgg ctacttttca aattaaagaa aaaatgtttt
    24121 ctctaggcgg taaatttact attacagacc tgactgggct tccctgttat cacgttgaag
    24181 gaagtttgtt ccctctacct aagactttta aagtctttga cgaagaggaa catctcatta
    24241 gtcagattga gaagaaggtt ttaagttttt taccaaagtt taatgtgaca ttagccaatg
    24301 ggaatcattt cacgattaag aaagatttct catttttgaa acctcattac accattgaag
    24361 accttgatat ggaagttaag ggaaattttt gggatatgga tttccaactc ttaaaagaca
    24421 atcaagttat agcaaacata tctcaacagt ggtttcgtat gacttcaact tatcaggtag
    24481 aagtttacag tgaaacttac aacgatttga caatttcgct tgtcatagcg atcgactatg
    24541 tcaaagagtt agaaaaaaat gcatcaaact aattttagga gagaataatg aaagaattat
    24601 cgtctgcaca aatccgccaa atgtggttgg atttctggaa atccaaagga cattccgttg
    24661 agccttcagc taacttggtt cctgttaatg acccaacgct tctttggatc aactcaggtg
    24721 ttgcaacctt gaaaaaatat tttgatggtt cagtgattcc agaaaatcca cgtattacca
    24781 atgcacaaaa atcaattcgt actaatgata ttgaaaatgt tggtaaaaca gcacgtcacc
    24841 atactatgtt tgaaatgctt ggtaacttct caattggaga ctatttccgt gatgaagcta
    24901 ttgagtgggg atttgaactc ttgacaagtc ctgaatggtt tgacttccct aaagacaagc
    24961 tctacatgac ttattaccca gatgacaagg attcatataa ccgttggatt gcttgtggcg
    25021 ttgaaccaag tcacttggtg ccaatcgagg ataacttctg ggaaatcggt gctggtcctt
    25081 caggtccaga tactgagatt ttcttcgacc gtggtgaaga ttttgatcca gaaaatattg
    25141 gacttcgtct tttggctgaa gatatcgaaa acgatcgtta catcgaaatc tggaacattg
    25201 ttctctcaca attcaatgct gacccagccg taccacgttc agaatacaaa gaattgccaa
    25261 acaaaaacat tgatacgggt gcgggtcttg aacgtcttgc agctgttatg caaggggcaa
    25321 aaacaaactt tgaaactgac cttttcatgc ctatcatccg tgaagtagag aaattgtcag
    25381 gtaaaactta cgatccagat ggcgacaaca tgagcttcaa ggttatcgct gatcacatcc
    25441 gtgcgctttc atttgccatc ggtgatggtg cgcttcctgg aaatgaaggt cgtggttatg
    25501 ttcttcgtcg tcttctccgt cgtgcggtta tgcacggtcg ccgtcttggc atcaacgaaa
    25561 ctttccttta caaattggtt ccgactgttg gacaaatcat ggaaagctac tatccagaag
    25621 tgcttgaaaa acgtgatttt attgagaaaa tcgttaaacg tgaggaagaa acatttgctc
    25681 gtactatcga tgctggtagc ggtcacttag attcattgct tgcgcagctt aaggctgaag
    25741 gtaaagacac acttgaaggt aaagatatct tcaaacttta tgatacttat ggattcccag
    25801 ttgaattgac agaggaattg gcagaagatg caggctataa gattgaccac gaaggcttta
    25861 agtcagccat gaaagaacaa caagaccgtg cgcgtgcagc tgttgttaag ggtggttcga
    25921 tggggatgca aaatgaaacc ctagctggta ttgttgaaga atcacgattc gaatacgaca
    25981 catatagtct tgaatcaagt ctttcagtta tcattgctga taatgaacgt actgaggctg
    26041 tttcagaagg tcaagccctt cttgtctttg ctcaaacacc attctatgct gaaatgggtg
    26101 gacaggttgc tgacacaggt agaatcaaaa atgataaggg tgacacagtt gctgaggttg
    26161 ttgatgttca aaaagcacca aatggtcaac ctctacacac tgtaaacgtt ttggcatcac
    26221 tttcagttgg aacaaactac acacttgaaa tcaacaaaga gcgtcgtttg gctgttgaga
    26281 aaaaccacac agcaactcac ttgctccatg cagcccttca caatgttatc ggtgaacacg
    26341 caactcaggc tggttcattg aacgaagaag aattcttgcg ctttgatttt actcactttg
    26401 aagcagtaag caatgaggaa cttcgtcaca ttgaacaaga agttaatgag caaatttgga
    26461 acgctcttac aatcacaacg actgaaactg acgttgaaac cgcaaaagag atgggagcaa
    26521 tggcactttt tggtgagaaa tatggtaaag tggttcgtgt ggttcaaatt ggtaattatt
    26581 ctgttgaact ttgcggtgga actcacttaa ataattcttc agaaatcggt ctcttcaaga
    26641 ttgtcaaaga agaaggtatt ggttcaggca ctcgtcgtat tattgcagtt actggtagac
    26701 aagcttttga agcttatcgt aaccaagagg atgcactaaa agagatcgct gctactgtaa
    26761 aagctccgca attgaaagat gcaacagcta aagtacaagc tcttagcgat tcacttcgtg
    26821 atcttcaaaa agaaaatgta gagcttaaag aaaaagcagc agcagcagca gctggtgatg
    26881 tctttaaaga tattcaagaa gctaagggcg tgcgcttcat tgctagtcaa gttgatgttg
    26941 cagacgcagg tgcacttcgt acatttgctg ataactggaa acaaaaagac tactctgatg
    27001 tgcttgttct cgtagcagct attggtgaga aggttaatgt ccttgttgca agcaaaacta
    27061 aagatgtcca cgctggtaac atgatcaaag gattggcacc aattgtagca ggtcgtggtg
    27121 gtggtaaacc agacatggct atggcaggtg gtagcgatgc aagtaaaatt gcagagctgc
    27181 tagcagcagt tgcagaaaat ttataagaag tacctctcat ttagttagta aataaaacag
    27241 gcaaagggca gaacatcgtt ctgttccttt gtgtatatag gagaaatatg gttggtttag
    27301 gaacagttat caatgttatt cttattatag ttggaggctt tgtaggttta tttttgaaaa
    27361 attttttgaa agaatcactt caaaagagcc tcatgcaagc catgggagtc gcagttttat
    27421 ttatttcaat atcaggtgtt ttggagaaaa tgatgttagt cgaaaagtca catctcatct
    27481 ctaatcatac aaatatgatg attattactc ttgctctagg tacggtactt ggagagttgc
    27541 tatcactaga ttcgtatatt gatagatttg gggactatct taagcaaaaa acaggaagtg
    27601 gtaacgatat taaatttgtg gaagcattcg tcacctcaac ttgtactgtt tgtattggag
    27661 caatggccgt tgttggtagc attcaagatg gtatagcagc agatcattcg attttatttg
    27721 ctaagggaat gttagacatg attattattg caattatgac agtgtcttta gggaaagggg
    27781 ctctattttc tgcacttcct gtcgctttac tacaaggtag ccttacgatt gttgcttttt
    27841 tcatggggag cctacttaat ccatcatctt tagattacct taacttagta ggaaatatgc
    27901 ttatattctg tgtcggtgtt aatttactct ttaacctaaa tattaaagtt atcaatatgt
    27961 tgccagcaat tattttagct atattatggg gaagttttat ataaaagata accaagtttt
    28021 aactcaaact tggtttttct ttagacatat gttaggatga aagaagatat actacggaga
    28081 ggatccaact catgaatcta ttattttcta ttgatgatat gtacgtagat cattttaaag
    28141 tgatgcttta ttcgcttgtc cgacaaacca agaatcgtaa gttagagata tatgtcttac
    28201 aaaaaacatt actcaaacgt catacagaac ttattcaata tactcaaaat ttagaagttg
    28261 gctatcatcc aataattgta ggtaccgaag tatttgctca ggcacctaca actgatcgct
    28321 atccagatac aatatattat cgacttttgg ctcataaatt tttacctgag accttagata
    28381 ggatacttta tttagatgca gatatgcttt gcttaaatga tttctcaagt ttatatgaca
    28441 tggagttagg tgatcaacta tatgcagcgg caagtcataa tactgatggt aagtttctag
    28501 attatgtcaa taaattacgg ttaaaaaatg tagagctaga atcctcttat ttcaatacag
    28561 gggtactctt gatgaacttg cctgctatac gaaaagttgt tcatcaacaa actatcttag
    28621 attacataat gcaaaaccgt ggtcgtctaa tcttaccaga tcaggatatc ttaaatggct
    28681 tatacgctaa tttggttaaa ccaatacctg atgaaattta taattatgat gctaggtata
    28741 gtcttattta tcaattaaaa agtcgcaatg aatgggattt agagtgggtt attaaccata
    28801 ctgttttttt acattttgca ggacgagata aaccatggaa aaaagattat agaggacgtt
    28861 actcgggcct ttataagttt atggctaaag aggctttaac taaaaattga agatttaaaa
    28921 ggttactaaa ttatttctat ttagtaactt ttttgggggt gatattttaa tgatatcaat
    28981 aatctttgct aataattatt tataaaaaag aaaaataata tcaaaggagg agcgtgttaa
    29041 aatcacttaa tcattgaaca atcaaattaa tcattgtttc aaaagcataa tggttttagg
    29101 agttactcaa atgaaaaagg aatttattat tacaggtcgg atggttgcaa gagttatgga
    29161 aaccctagtt tgcttatgta tggtaagtaa tcttgttctt gatatgcttg atataataga
    29221 aataaatgaa attgtatttt gtgtgttggt agcatcaatt gcagggcttg agttttgttt
    29281 gcaaaaagaa agggagtaga tagatggaat ttttggctta atggcttttt atggtcatga
    29341 cgtatattgg tgtacctaaa gagcaaaagg cactcagtcg taggggtaaa caattagaaa
    29401 aaataaatca gggaggaaaa acacttatgt cacaaatgat tcaagattta gtaggaagca
    29461 catgtatgat ttatttagag gatggttttc aaacgcatcc ttatgagatt ttaaaggtag
    29521 atgaggattg ggtacagatt cgtcgcaact tgaaacgaag taaagaggag gtacgtattc
    29581 tccgtattca tgatatcaag gaattgcgtc cagtcaataa atagagtgag gaataaatct
    29641 atatatcatt ttataaagga gaaaaatgat gggattaatt ttaaaaaaca ggctcaaaga
    29701 attgcgtgca cgagatggta tcaatcaaac agaactcgcc aaattaacgg gagtctcaag
    29761 acaaacaatt agcctaattg aacgcggaga atatacgcca tctgttatta ttgctatgaa
    29821 gatttctcaa gtatttaaag aatccctaga gaatgttttt agtttagtgt caacggagga
    29881 ataacgatga aaaattttat aaaacaaagt gatactacat ttgttcgaat tatcaaaagt
    29941 cttttaattg gtggttttat aggggccata cttggttccg taggagctct ttttaacata
    30001 tttggtcaag ataagtactt atctgaaatt aatattgttc aatatttcct atgggttagt
    30061 cgtattgttg taattataac agctctcttt agtctcatct acctctatca gattcaaaaa
    30121 tatcagaaag tttttttcaa tgttgatgaa agtcaatctg aagaaattta tcgccagatt
    30181 aatttaagac attcttat
//
LOCUS       DATRNX010000010        28626 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRNX010000010 DATRNX010000000
VERSION     DATRNX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28626
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(158..886)
                     /gene="lrgB"
                     /locus_tag="VBC81_000374"
     CDS             complement(158..886)
                     /gene="lrgB"
                     /locus_tag="VBC81_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6911439.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(888..1343)
                     /locus_tag="VBC81_000375"
     CDS             complement(888..1343)
                     /locus_tag="VBC81_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6911440.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(1513..2253)
                     /locus_tag="VBC81_000376"
     CDS             complement(1513..2253)
                     /locus_tag="VBC81_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6911441.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(2234..3979)
                     /locus_tag="VBC81_000377"
     CDS             complement(2234..3979)
                     /locus_tag="VBC81_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6911442.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(4006..4650)
                     /locus_tag="VBC81_000378"
     CDS             complement(4006..4650)
                     /locus_tag="VBC81_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6911443.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(4774..5169)
                     /locus_tag="VBC81_000379"
     CDS             complement(4774..5169)
                     /locus_tag="VBC81_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6911444.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            5250..5966
                     /locus_tag="VBC81_000380"
     CDS             5250..5966
                     /locus_tag="VBC81_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6911445.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(6020..6646)
                     /locus_tag="VBC81_000381"
     CDS             complement(6020..6646)
                     /locus_tag="VBC81_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6911446.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(6679..7002)
                     /locus_tag="VBC81_000382"
     CDS             complement(6679..7002)
                     /locus_tag="VBC81_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6911447.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(6999..7283)
                     /locus_tag="VBC81_000383"
     CDS             complement(6999..7283)
                     /locus_tag="VBC81_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6911448.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            7444..7683
                     /locus_tag="VBC81_000384"
     CDS             7444..7683
                     /locus_tag="VBC81_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911449.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            7868..8935
                     /gene="pepA"
                     /locus_tag="VBC81_000385"
     CDS             7868..8935
                     /gene="pepA"
                     /locus_tag="VBC81_000385"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6911450.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            9005..9775
                     /locus_tag="VBC81_000386"
     CDS             9005..9775
                     /locus_tag="VBC81_000386"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6911451.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(9796..10461)
                     /locus_tag="VBC81_000387"
     CDS             complement(9796..10461)
                     /locus_tag="VBC81_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6911452.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(10530..10985)
                     /locus_tag="VBC81_000388"
     CDS             complement(10530..10985)
                     /locus_tag="VBC81_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911453.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
     gene            complement(11026..11163)
                     /locus_tag="VBC81_000389"
     CDS             complement(11026..11163)
                     /locus_tag="VBC81_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911454.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(11222..11428)
                     /locus_tag="VBC81_000390"
     CDS             complement(11222..11428)
                     /locus_tag="VBC81_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6911455.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(11579..12772)
                     /locus_tag="VBC81_000391"
     CDS             complement(11579..12772)
                     /locus_tag="VBC81_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6911456.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(12804..13778)
                     /locus_tag="VBC81_000392"
     CDS             complement(12804..13778)
                     /locus_tag="VBC81_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6911457.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(13893..14116)
                     /locus_tag="VBC81_000393"
                     /pseudo
     CDS             complement(13893..14116)
                     /locus_tag="VBC81_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14094..14486)
                     /locus_tag="VBC81_000394"
     CDS             complement(14094..14486)
                     /locus_tag="VBC81_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6911458.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLCKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(14475..14550)
                     /locus_tag="VBC81_000395"
                     /pseudo
     CDS             complement(14475..14550)
                     /locus_tag="VBC81_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(14547..15638)
                     /locus_tag="VBC81_000396"
     CDS             complement(14547..15638)
                     /locus_tag="VBC81_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6911459.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(15523..16455)
                     /gene="tadA"
                     /locus_tag="VBC81_000397"
                     /pseudo
     CDS             complement(15523..16455)
                     /gene="tadA"
                     /locus_tag="VBC81_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(16627..16992)
                     /locus_tag="VBC81_000398"
     CDS             complement(16627..16992)
                     /locus_tag="VBC81_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6911460.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(17106..20756)
                     /gene="rpoC"
                     /locus_tag="VBC81_000399"
     CDS             complement(17106..20756)
                     /gene="rpoC"
                     /locus_tag="VBC81_000399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6911461.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(20873..24448)
                     /gene="rpoB"
                     /locus_tag="VBC81_000400"
     CDS             complement(20873..24448)
                     /gene="rpoB"
                     /locus_tag="VBC81_000400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6911462.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(24972..27269)
                     /locus_tag="VBC81_000401"
     CDS             complement(24972..27269)
                     /locus_tag="VBC81_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6911463.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHTFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            27380..>28626
                     /locus_tag="VBC81_000402"
     CDS             27380..>28626
                     /locus_tag="VBC81_000402"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6911464.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFV"
BASE COUNT         9126 a         5799 c         4621 g         9080 t
ORIGIN      
        1 taactggtta attagaaaat ttgttatttt atcattaata acttaaagtt aggtaaaaca
       61 caattctaga ctctagctca taaaccatgt taatccaatg tattttaata gacatcataa
      121 aaaggctgaa taaattcagc cttaaccacg ctattagtta tagcaaacca atcaaatgac
      181 taaagatagg aataaccaaa tcaactacca aaccgataac caccaccgct atagctgcca
      241 tcgcaccttg cagttctccc aattccaatg caaaggctgc tccgactgta tgaccagagg
      301 tccctagacc taatcctgca ccaatcttac tatcattcaa atggaaaaat gacactaatt
      361 ttttacctaa tgcataaata ataactgcat ttaagataca cgccattgct gtcacagctg
      421 tgttccctcc aattgctgct gttattggaa gagcaattgc tgttgttgct gcttgtggca
      481 acattgaagc aattccgact tgactaagcc caaccatttt agaaatagcg acgataagca
      541 taagggaaac aatcataccg attactaaac tgctgagaat ttctacccaa tattttttaa
      601 caacgtcatt tttcttatag agaggaactg caaaagcaat tgttgctgga tttaaaaacc
      661 aaaatattaa atcgccacct ggtttataag cttgtgtata aaatgtctta acatcggttc
      721 ctaaaccttt tgacataagc caaagaatga caataccact aaccattgca aagaaaagag
      781 gctgcaaaag gaagaaacct ttactcttct taaataaatg ttgtcctatc gtatagagta
      841 ttaaagaaaa gcaaatacca aagatgggtg tttttaagag ttccatatta tttttcctct
      901 actttcttac tatagtggtt aggtaatatc tttttaaata aatctgtgac actgagttct
      961 ctgatagcaa gtattaatcg tgcaacgtat gctaccacta ctaacataac aacagttgag
     1021 attgtaataa cagcaactag ttgcaagcct tcagctttta ggatatcgag gttcgctgct
     1081 actgatattc ctgaggggac aaacataaaa ccaatcatgc ttatcatcaa agccccaaaa
     1141 gagtcaaccc actctacctt gataatttta gcagttagca atacgtacat tagcaccaaa
     1201 ccaataaccg tcgttgggat tggtagtgat ttcggtaata acattgaaat catttgtgaa
     1261 actaatagaa tagctgcata tattgacatt tgaataaaca ttggagcttc ttttgttttg
     1321 gtttctttac tggtgtgttt catagtcact accccttcct tttattacaa tgttttaact
     1381 tgttttattg atagctatat tgtacttcaa aataaataaa acgttttcat tttcagcctc
     1441 agttaccaaa aaaagaagcc gaattgcaaa ttcaagcttc taaacaacaa agggaagttc
     1501 gcaaaagcac ttttacatgg ttaaatgctc atttaggtct tttaaatacg atcgacctac
     1561 tggaaatttc tctccattag acataattag taacaaagta tgattaaacc aaggttgaat
     1621 ttcctcaatc atctctagat taatcactgt gtttcgatga atctggagaa aatatggtgc
     1681 tacggcgcgt gacttaaacc aattgagtgt tttccgaata gtgtaagttt tctgaactgt
     1741 actaacagtt aattcgccat cttcaacact agctgcaacg atatcttgca ttttaacaac
     1801 aacactgcga tctgataatg tcaaggttaa caattcagca ctggcttttt taggtattgc
     1861 ttgctctaca tctgaagcag tggtgagttg gctcaatttc ttcacttttg atagagccat
     1921 atcaacgcgc tgttgttcaa acggtttcat gatataatca acagcattac tttcaaaagc
     1981 tttcacagcg tagttatcat aagctgtcgc aaaaacaaca agtgggggat gtgcaagttg
     2041 gctcaactga ttagccaaag taaagccatt ttcttcacta agcgaaatat ctaaaaaaat
     2101 taaatcaatt tgctgtctaa ataagatttt ttcggcttca ctgatatcct cagcctgaaa
     2161 aatctcaggg ttgtcaacct cctggctatg ttcaactaaa aacgataatt cttgtctggc
     2221 aaacatttca tcatcaagaa ttaaaatttt catgctcatc ctcccttatt ctattaggta
     2281 ttcgatacca aacttttgta ccattcttgt cgctcgaaaa atgaaggcaa cttacactac
     2341 catataataa attcagcctg ttatttagat taactagagc agtacctgtc cccttactct
     2401 ctgcaactgt ttcttgacct aatttatcaa tgatagtatc tgagattcct tgtccattgt
     2461 cactaacaga aacacaataa taatgaccat ctggctttat ttgaaccaat atatggttgt
     2521 ccgtcttacg ttctttgaaa gcatgtcgaa ctgcattctc taccagtacc tgtaaaccaa
     2581 aaggcggtaa cttcattttt tctggtgcac taatatcata agataactga tatttatcag
     2641 ggaaacgtaa tttttcaaca ttcatataag catccacatg tgatttttct tgctcaagcg
     2701 ttacctcacg atcctgacca ccttgcaaac ttgttctaaa aaaagtactt aactgcatca
     2761 gtgcataacg tgctttatca gaatcaatac ggattaatgc actaattgtg ttaatggcat
     2821 taaagaagaa atgagggttg atttgtgctt gtaaagcctt tatctctgcc atactggcta
     2881 acttattttg ttcctctgtt atccccattg ccagttgtcc tgaaaatatt tgcgctaaac
     2941 caaggactag gttttcctcc acctcagaca ttgtcttatc tcctgcaaag tacattttta
     3001 aggcacacac agttttatca tttattttta gaggaactac aatagcagaa tttaactgac
     3061 agttgtgatc tggacaagaa atcgccgctt tatcttgcgc aattcttggt tcgccatcaa
     3121 aaataacact tttagataag tctgttttga ccggttgtcc tgcaatatgg tgatcatggc
     3181 caacaccaat atgagctaat acgtttgacc gatctgttaa tcccacagca tcaaagttag
     3241 tatgcctctt tataatttcg caaacgctcc tagcagattg cggtgtcaaa ccttgtctaa
     3301 ggtagggcag agtctgtcga gtcaattcaa gaacatctct cgtttgaact gcgcgtaact
     3361 gactttcatt tgacaaataa gttttcaaaa tcgcaaggaa aagtgtggaa cctaaactat
     3421 ttaaaatcat cattggaatg acaatcattt tgacaagttc ccatcctgta aaaatgccaa
     3481 caaatagcat ctggatactt tcggcaataa tactaattaa aataacttgg cttgttgaag
     3541 ggtagagatg gttttcctta agcttatcac caatctttcc gctaacaatg ccgactagaa
     3601 ctgaactgac aatatagaaa gaacctgaaa agcttccttg aaaaaagcga tgaactcctc
     3661 caataaaacc aacaattgat ccaaccagag gtccaccaac caaacttgcc gttgtaataa
     3721 ctaaagtcct tgtattagca agtgagtcag aatgggaaat cgttgttaga aaagggcgct
     3781 cgaccaaact tcgatcccct tttatttcaa ttcctgttat attagatata ataacaaaca
     3841 agccgaaaat gatgacaagg actaccgttt cacgtttaga ccgctcttca attaactgtc
     3901 tgaaataact attatttacc aataaaaagg ctaaaatcat aataattcct agcctttgga
     3961 ataacaacac catcaacata tgcttatcct cctcaagata ccattttact ccggaagata
     4021 aatatagata tcttgataat ctgaaataac cttgtctgcc attgataaat cttgcggtgg
     4081 ataatcagga ttggcaaagc caaagcaata cattcctgct gccttagcag ctaaactccc
     4141 atttcttgta tcttctataa ctatacaaac tttagggtct acatctaaaa gctctgcagc
     4201 tctaagaaaa acatctggtg ctggtttaga agacgataca tcttctcctg taaccatgta
     4261 ttcaaagcat tctgtcacac ccaactcttt tagattacgc ttaatatcta ccattggaga
     4321 tgaggacgca actgctaagc gatagccatg ttggtgtaac caatgaatca gttgctgagc
     4381 tcctttaatg ggcctaacac catcacgcgt cacaatcgct tgtcgacgtc tgttcatctc
     4441 agcaatatac tcttttactg ttttcggtaa gccaaactcc tctttcatag cttgccacat
     4501 gaattcaaaa gtagtcccca tgtactggta ttgatacgag acgtccgtat caataccctc
     4561 ctcacgtaac atttctgtct tattatctaa aaacgtgtac tcggaatcaa caataacgcc
     4621 gtccatatca aaaataatga ctttttccat cttaaacctg ctttctatcc cctttattgt
     4681 aatcgtttgc ctttctattt tcaataaaaa aaggttgctt tctgtggaaa gcaaccagta
     4741 tatttagaaa tgatattttg agagtgattt tatttaaaag gggagctcct cttcttccaa
     4801 tactaaatct gacaaatcac cagaaacatt attttcacgc atagcacgtt gggcacggct
     4861 ttctaacaac tgaaatgatg atgctaatac ttcagtgata tagtgcgttt gaccatcctt
     4921 ttcgtacttg cgcgtacgca attcaccatc tattgaaatt aaagagccct ttgtcccata
     4981 gctcgcaagg gtttccgcta gacgacccca cataaccaca ttaataaaat ctgcttcacg
     5041 ctcaccatta cttcctttaa agcgtctatt aacagcaaca gttgcacgcg ttactgactt
     5101 gtcagttggt gtttttacca tctcaggctt tgctgttaga cgcccaatca taataacttt
     5161 attatacatt gattacctcc tcactattat tattcgaaaa taaggaagaa aaatgctaaa
     5221 atatatgaaa ttgttctggg agaattaaga tgacaaatgt aactagttat aaagaaatgc
     5281 tggctaaacc ttggggaaaa atacaatatg aaataacttt tgctcaatta agccatatca
     5341 aaaatcagaa cgttttagat tttggagctg gtttttgtct caccgcacaa catctagcaa
     5401 aagaaaacaa tgtaactgct attgaaccca atcctaaact gctttacgat aatcaaagtg
     5461 acaatattta taaaatccta ggttcctacg aagcattaag agctttgcct gatcaaagtt
     5521 ttgatactat tatttgtcat aatgttcttg aatatataga caaacataat catcctgctt
     5581 attttgacga attttctcga cttctaaaac ctaatggaga actatctctc atcaaacata
     5641 atataattgg taaaatatta caatcagtta tttttagcaa tgatacctcc actgcgatgg
     5701 aactcttaac aggagaagca aactttaaaa gtgccagttt tgaccaggga aatatttata
     5761 ccttagaaga actaaaacaa aatacaaatt tattagttga acgttatcag ggtattcgta
     5821 ccttctattc attacaacca aaccatttta aaactgaaac tgattggcta aacaaaatgc
     5881 ttgctatcga gctaagtgtt gctgacaaag ctccttataa agatattgct tttttgcaac
     5941 acatcacact taaaaagtca ttgtaacttc ttaagtttac aactaaaaat caacttagct
     6001 gaagctaagt tgatttttac taatttttcc aatgtttatt ggcgtcgaaa gactctccaa
     6061 caattacctg atcagataat tcaatgatac ctctgacttg aggagcattt ggaagagcta
     6121 actcgcgagc actacataac attccaaagc tatcttctcc acgaagtttt cctgggaaaa
     6181 taaggctacc atttggcatc atagctcctg gaagagctgc aactgtttta aggcctactg
     6241 aagcattagg tgccccacaa acgatttgaa ccgtcttccc atcgttgatt tctacttgac
     6301 agatgtgtaa atggtcacta tctggatgat caatcatttc tttaatttgc gccacaacaa
     6361 atgtaggttc aatattatta acaagagagt cttcgaatcc ttctttcagt agttctgcat
     6421 taaggatttt aataacttga tcagataaag ttacttgacc tactccctca atagcaatta
     6481 aacttgacac ttcaaagata ttccaagcaa ctgtttcttt actatcttgc agatagacgc
     6541 gtgctacttg tccgcgacga tcaacatcta gcttagctcc ttgactatcc ttaacaataa
     6601 ccattaaggt atcaccaaca tgctctctat tataagtaaa aatcatttat tctcctttaa
     6661 attttgctat tttttgtctt atttatggtt aatttcagcg agaaatttag taatttcagc
     6721 ctttgttttg cgattcttat taaccaaacg acccagttcc tgaccatttt ccacaacaac
     6781 gaaactcgga atcccaaaaa tattccactg ctgagctaat tctatatagt catcacgatt
     6841 aacacgtaca aagacaaaat ctgagaaatc tttttcaatt gatggcatta ctggatatat
     6901 aaattgacaa tctggacacc aatctgctgt aaaaaagaaa acaactttct tagtgctatc
     6961 aatataagct gctatttctt cataagattc tggtaaaatc atttgctcac ctccacataa
     7021 tcaagaacac cttgacgata aatgaattcg aaaattctgc catcttccaa gacaagacca
     7081 ccactggtca ttttaatatc agattcaaat tcattaaaat aaaatacctg aatatcaccc
     7141 atctgactaa aaagttctct taaggtctga gtgatttgct gacgtttata atctgttact
     7201 tttttagtta gggtataacc agcagctgcc agtaaaccca caccagcaag gccagtccca
     7261 aataagattt ttttattttt cataactgtt attataccat accccccgga attagacaca
     7321 ggaattgatg gaagaaatcc acataacagt tatgaaaaaa aacaacttat tccttgtggt
     7381 ataataggat gacattgtct taagatacca ttatggttaa tcattcaaac agaaagaaaa
     7441 aacatgaaag aaaaattatt agtacctgct ttaaattgtt tgctaggtct actcttccta
     7501 ggtgatcact ggtttttatt tcgtcttcaa aatggagaac tttttgctat tgtgacgcta
     7561 atactgcttt gcacagcttc atttttaatc aataagaatc accccaaaaa tttgacttta
     7621 attgctatgg ttatcacttt tttagccgta ttatctttcc tatctacctt tttattagct
     7681 taagccgata aacattatct ccgttgtatt caacccagtt ttcactataa caggctttgt
     7741 cagtcataac agtcttctgg tagtacttga tattcttttt gaaagtcttt atattgactg
     7801 aaacctgttc tgttctttat ttttaattaa aaggtgctaa aatagtattt gtagaattga
     7861 ggtaattatg tcagatttat ttaacaaaat taaaaccgta actgagcttg atgggattgc
     7921 tggctatgaa cacaatatcc gcaacttcct tcgtcaagaa ataactcctt tagttgatca
     7981 agttgagaca gacggacttg gtggaatttt tggagtaaaa aatactcatg agactaatgc
     8041 tcctaaagtc atggttgctg cccatatgga tgaagtcggc tttatggtta gtcatattca
     8101 gccagatgga acatttcgtg tacttgaggt tggaggatgg aatcccctag tagtcagctc
     8161 acaacgcttt accctctaca cacgttctgg tgatgccatt cctgttatat caggctcagt
     8221 tcctcctcac tttcttcgtg gacaaagcgg tggaacaaca ttacccaaaa ttagtgacat
     8281 tgtttttgat ggaggattca cagataaaaa tgaagctgaa agctttggca ttgctcctgg
     8341 cgatatcatt gttcctaaat ctgaaaccat tttaactgca aatcaaaaac atattatgtc
     8401 aaaagcttgg gataatcgct atggtgtgct tatggtgacc gaattgctaa aaagcttaaa
     8461 agatcaaagt cttagcaaca cacttattgc tggggctaat gttcaagaag aagtcggact
     8521 tcgtggcgca catgtttcaa caactaaatt caacccagat atcttcttag ctgtcgattg
     8581 ttccccagct ggagatattt atggggaaca aggcaaaata ggagagggaa ccttaatccg
     8641 tttttatgat cccggacata tcatgcttaa agatatgaga gatttcttgc ttacaacagc
     8701 tgaagaagca ggtataaaat accaatatta tgctgcaaaa ggtggtaccg atgctggggc
     8761 tgctcaccta aaaaatagtg gtattccttc tacaactatc ggtgtctgtg cacgctacat
     8821 tcattctcat caaacactct acgctatgga tgattttcta caagcacaag cttaccttca
     8881 ggccatcgtt aacaaattag accgctcgac ggtggatatt attaaaggtt attaagatag
     8941 aaaggatggg agtttcataa ggtagacaca ttttagtatc tcctttcatc cccatatcaa
     9001 cattatgaaa attggaatta tcggcgttgg aaaaatggcc agtgcaatta ttcaaggcct
     9061 caaacaaacc caacatgaca ttgtcatatc cggatcttgc ttagagcgtt caaaagaaat
     9121 tgcagaaaga cttgatgtta cttacgccga atctcatcag tcattaatca atcaagctga
     9181 tattatcatg ttaggtatta aacctcaact atttgaaaag gttcttcttc cacttgatat
     9241 cacaaaacca atcatctcaa tggcagcagg aatctcctta gcgcgtttaa gccaattaac
     9301 tcgttctgat ttaccattga ttcgtattat gccaaatatt aacgcacaaa tacttcaaag
     9361 ttgcacagct atttgttaca acaatcatgt atcagatgag ttacgacaat tagccaaaga
     9421 aattacggat agttttggaa gtagctttga tatagcagaa atcaattttg ataccttcac
     9481 tgctctagca ggttctagtc ctgcctatat ctatctattt atagaagcct tagctaaagc
     9541 aggtgtaaaa tatggatttc caaaagaaca agcactctct attgtaggac agactgtctt
     9601 agcttctagt caaaacctat tacaaggaca aaatagcacc tctgacttaa tcgacaacat
     9661 ttgtagccca ggcggaacta ctattgctgg ccttttagac ttagaaaaaa atggtctgac
     9721 gcatagtgtt atttctgcca ttgatgctac tattgagaaa gcaaaaaaac tttgagcaaa
     9781 ttgctcaaag tttttttagt tattcatcat tgtactgatt aacacgctat aagcaagagc
     9841 attgttaata aaatgaatca ttatactata ttctaagtgc tcagttttat aataaacaaa
     9901 agataaaata gcacccatcc cagcataaat aataaaacta cctatatcag aaggggaatg
     9961 aataagagca aaagctaaag taccgatgac aaaaccccat ttttgatgtt tcgcaaaaag
    10021 ttctttaggg ataatatagc gaaatataat ttcttccata attggtgccc ctattatggc
    10081 aaaaattaac atcaaagcta gtggaagttg ctttgttaat tccataatta ctgtttgatt
    10141 tgctgtatct ttaacccctt gcatagtcat aatgaggtaa ccaataattt gtggaaccct
    10201 caatgataac caacctaaga gaatagtaac taatgctttc cttgtcaata atactttcca
    10261 tctgattatt tgtttaacct tggcataata aaggaaaaga gcaataataa gtaattctaa
    10321 gccaattaac aatatactag taaaaggcgt ttgatagtga ttctttagga gccacacagc
    10381 caatatagga acaagttgat taataactaa aaatgctatt agcaaagcta aaattttgcc
    10441 ttttttaata ataaatgaca caaaaacctc caaaataaat tattaacttt tttctatcat
    10501 aacatatttt caaatcttat agatttatct cacaataact tctgtaaaat aaagtaaatt
    10561 aataaataac cgtataaaac aaaagagagt atcaatagca agaactttgc ataggaaaaa
    10621 gttataccaa accagacatt aatcagacac aataccacta aaacaaggac agataaaagg
    10681 aaaaggagtc gacaagacat attagcaata aattgattac gctcatcaaa aactttggta
    10741 tagtaatgtt gaaatcgctt ttcactaaat gttatcacca aaaaataaaa acttcctgca
    10801 aataaggcaa tacctgaacc aaaggaaaaa cctttagtaa aattagattc aaaggtatgt
    10861 agaaatgatg atactatcat aatcaaacta catacactaa aaacaaaaga caaaaatctc
    10921 atatatactt tgtaatcctt cggagatact ttactattgg gtgacaataa tccttttatt
    10981 aacatagaat actactttct aaaacttcaa tttaatcatc agctattata tcgttttctt
    11041 caatgaagca tagcgataaa tagcaaaaat agacatcgca atgctaatac taatagtact
    11101 gataaaaaca tattgctggt aaacgattgg tggacaaaaa tcaatagtcc acatccttga
    11161 catattacag attaactttt aaccttttgt cttttgtgga ctgtattttt ccattaaaat
    11221 tctatcctcc ttcactttct gtataaataa aaacttcctc aatttgttta tcaaaataac
    11281 gcgctatctt aaatgctagc tccaaagatg ccgtgtattt ctctttttct agagaaataa
    11341 tagtttgcct agtaactcca agtgcaactg caagttctgc ttgacttaat ttcctagatt
    11401 ttcgtaactt ttgtagagaa tttttcatat cttacacctt tttgtatagt tagctttaca
    11461 tttatagtat agttaactat acattttttg tcaagtacat tttacatacc tgttcaaaaa
    11521 acaaaaaagt tgatagttaa actatcaact tttctggtct tatcaatttt ttgataactt
    11581 atttagcttt aaggcgctca acgtcacgcg caatcattaa ttcttcatca gtaggaataa
    11641 ctaaaacctt cacttttgaa tctggttttg tgatatctcc aaaataacca aaaacatttt
    11701 tttctggatc aagttcaatg ccaaaccatg ataatcctgc gataacatct tgacgcatta
    11761 atggcgcatt ttctcccata cctgcagtaa aaataatagc atccgcgcca tttaatactg
    11821 ccaagtattg tccaataaat ttcttaatac ggtcaataaa gacattatag gctaacactg
    11881 catttggatc tttactttga agacctgctt caatatctcg catatcactt gaagtgcctg
    11941 atactccaag caatcctgat tgtttgttaa gcatattaac aacagcagct gcatcttcca
    12001 attctggatc attagctaca agataaggga taatcgcagg atcaatgtct cctgaacgtg
    12061 ttcccatcat tggtcctgca agtggtgtga atcccatcga tgtatcaata gactgtccat
    12121 gataatttgc tgtaatagaa acaccattac ccacatgtgc tgtgattaat tttaattctt
    12181 ctaaaggacg tcctaattgc ttagctgctt cttgtgcaac gtattggtgg cttgtcccgt
    12241 gtgcaccgta tttacgtact ttataatcag tatagtactt ttgtgggatt ggatataagt
    12301 aggtatgagg ttgcattgtt gtatggaagg ctgtatcaaa tacacataca ctcgtgatat
    12361 ctggtaagat ttctctaaac gcacgaatcc ctgctgcagc tgctgggttg tggagtggag
    12421 ctaaagctga taattcttca acctgttcta caactttatc atctactaaa gcagactctt
    12481 taaaatattc accaccagca acgacacgat gaccaacacc tgtaatttca ttaaagtctt
    12541 tgataatacc atgttttgtt agatcttcta aaagaatttt aacagcttgg gtatgatcta
    12601 cgatatctaa aatttgttca tcttttttat cgtcaaattt tacagtcgaa atagaatctt
    12661 tcaaaccaat acgttcgata atccctttgg caactacttt ctcctctggc atttcatata
    12721 attgccattt caaacttgaa cttccagcat taattgctat tgtttttgac ataatttacc
    12781 tctctaaagc gttttcacaa tctctattct atcatatttt ttgagtaatg actattttct
    12841 ctgctccatt tttgaaaatt ttcaatgaag tttgccatat tctcacgatt ttgcaaatct
    12901 gtcagcggat atacaaaggt ttctggtttt tgttctgctt gcttcttgag aacaaatata
    12961 gatttcgcat tttgacgact tccaaaaata gtttctggta gagttaaaac ggcaatgaca
    13021 tctgcatatc cttttaacca ctccttcagc aaatcacttt gtggacttgt taaaaggttt
    13081 tcgggtgcta aaaatatagc gattccatct tttttcaaat atttaagaga ttgctccatc
    13141 aatagatggt gagcataggt gtgctcttta gaacttgata cagcatatcg tttagcaata
    13201 tcatcattag gataatagcc aactggtaaa tcactaatga ttacatcgct ttctttcaaa
    13261 atttgtggtc taacggcatc ctcttggata aattgggcac tagaacctat aatttcagca
    13321 atgcttgctg atagatcaat caaaagatca tcaacttcaa tgcccatata atttaactct
    13381 ttcgagctgt tattgaggag agtctgagct aaattcccag ttccacttcc aatttccaag
    13441 acatccactg tctcttgact cgttaattct tccaaaagaa ataacaagat aaaaccaata
    13501 ctatctggcg taaactgatg attagcttgt aattgctctg tttgcgcaga tttgataaaa
    13561 atgaactgga aagtgcgacg ccattcttct tgacttaagt caagttgacg taatttttgg
    13621 ttattcacca caacaatatc taaatcacaa cttgaaccaa ggtaataaga gttctgttca
    13681 attaaggcat cataaatatg agtttttaat tggttctcaa tcgtttggat attttctaaa
    13741 atcagctcat aggctgtctc aattttttca aaattcatga tttcctccag aatttatcat
    13801 aacaaaaaag acagcatctg aaaagagaag caaacaaaaa tcctgttttt aagcaaaacc
    13861 ttgtcttact aatatttcat cactgtatat ttttaatgtt tatggcttgc tttattatca
    13921 ttagacttga gaggtacttg gagagtgtct attttacgat aaaacttatg gttatagttt
    13981 cctcccttat cctatgatac tgtctttctg ttgccaatat tcttgctaga taaaactgta
    14041 agacgagtgt aaatacagca gccaatacaa tagcttgcaa aaggatgcca gctttaagtt
    14101 tcttctttaa aatcatagta aaatgtcctt tttaacccgt ccttaaaata aaaaacaagt
    14161 tttaccatac ttttggtctg actcatttga caattgtcta acccataaac cattggttga
    14221 taacctcgac cattataacc tgtcttacgg aaatcatctt tattagattt gccaaaggtt
    14281 acaatcttat cttgcttaca taaataaagt ttgttctgtc ttaaatattc caagtgagcg
    14341 ccttcgaatt ctgcatttag ttgctgagtt aataacaccc attcagactg actggaagaa
    14401 gacatcacta ctatctgttg agccaacaat tttgttagtc cttgataaac tagcaaagct
    14461 cctgtgattg taaccaatgc taccaaacat tctaaaatgt gtaaatgctt taaccttctt
    14521 atccttacat tttaacaata aatttttcat gataatatct ccatattttg atacattggt
    14581 aacagcattg ctgcataaat cataacaatg ataagagcta caaaaataaa aataacgggt
    14641 tggattaatt gggtcgctct agctaatttt gtaaaaaaat cctcccactt ctcatcagca
    14701 tatatatcta actctgttcc taatttcgcc ttaacttgac catattcaat cattaaacta
    14761 agctcagtta agaaaaacgg atagtctaat actttttggt gaaatgcttt acctgataga
    14821 aaaccttctt ccatgtcata tcctatttcc ctaaaaagtt tggatttctg attttgcatt
    14881 actttaacaa tttggtccaa ttcaacacct tgacttaata aatttcccca ctcacgggca
    14941 tagtaagcag ttaaataaag cttaacatat gatccaacta agggaattgt tgttaaaaaa
    15001 caagctactt taatgcgcga caatcgcttt tgaataatat aaaatattaa actaaaaata
    15061 agtacaactg ctaaaagtaa taagaaaata ttcggcacat ttgtaatcag tctagttgca
    15121 aaattatttt ctcctaattg gggcattaaa taatgcctaa ggccaatcat aattagcacc
    15181 agaaaagaca ataatatcaa tgggtaagta gctacttcta ttactttttt tctaactaac
    15241 aaaagattag ctaaataaga ctcaatcttt agtaggctcc ttgaaatgtt accatgcaaa
    15301 tcagctaatg caacctgtgt gataacagtg tcagaaaaac ctaactctcc taacattttt
    15361 gataaatctt ttccctctaa taatgcctta ttcatcctat ctgtataaca atcagacaat
    15421 aacttactcc tctttaagaa ggtgaccata tcagttaaag aaaaaccact agcaaaaaga
    15481 ttattaaaaa gttggactac tttccgttgt ttcttgaggg ataatttttt cgacttgtgc
    15541 ctgtttctta ctgatatgtc cttcttcagc caagatatcc acttgtctat tccacttgtc
    15601 tgatgagtgt tttttaaagt tacttgtctc aaagtcaatt aggcttcctc ctccaattaa
    15661 acgttgatat gctattaatt ttagactatt ttctaactct tgatagttaa cccctaattc
    15721 tataagccta tcatagactc cgggaatact tttagcatga atagtagaaa aaaccattac
    15781 tcccgttaaa cttgcacgaa taacagcacg ggccgtcgct tgatctctaa tctctccgat
    15841 aattaaaata tctggacgat gccgtaaaga cagtttgatt aaagcatcat aagtcattcc
    15901 aatatcctca ttcaattgga gttgtaacat cttgtcattc ttgatttcta ccggatcttc
    15961 aatcgtgata atttgcttat ttttaaatac ttctgaagct aattgataca tgagagttgt
    16021 tttaccactc cccacagggc cggaaaaaag atatagccct cttgcacaca gtacttcctt
    16081 catttgcttt atattatcaa accaatattt taagtcctga tgacctgaat acaaagtacg
    16141 aataactaaa gattcttgac cacgataatc tcccacactt gatagtcgta atgaaaccag
    16201 tcttccctct gacagttcat agtcacaaga acctaattga cttcgtcttt tttctccaac
    16261 gttcatgcct gccacaaatt taaagtgact aataagacta gccatcctat taaactcaaa
    16321 aacatcaata aaccgccttt catcatcaat acgcatatag aattcataac aatcaccttt
    16381 gggaatgata taaatatctt gagcatttac ttctactgcc tgatgaatga cgtgctttgc
    16441 taatgattga accatcccgt acctccttaa ccatatattc gaaaataaaa taaaaaaatc
    16501 tcaccaattg gtgagatttt ttcaatgatt tcttgagggg tagtaacata aatattatga
    16561 agcagaggct gaacaaacct attatatccc cataaaaact atctaacttt tttaaactac
    16621 ttttaattac aaatttaaag aacatgccga tggcaactca gctatcttat taaatacttc
    16681 aaatcgctca atagattctt ccttaggaat ttcttgccat tctttcaata acattaagga
    16741 atgatactgt tggaggtact catcacattc ttcacaccaa cgtttttctt ttatactcca
    16801 aaacgttgcc aacaaactag acttactttg aaaataaggc cacttttcct gtcccctatc
    16861 ccactcctct tgaaaataga gcaaagcttc tgataaggta tcatactcag caatttcagt
    16921 aatatcttcc tcccaacctt caataaacca ccatggttcc caatcaccaa acatttttac
    16981 aacttgatac ataatacact tcctttagag agtattatat aaaaaataag aataaaataa
    17041 aaaatatctg ctcattccaa attcttgtaa aaaaagaacc tgagttaaac tcaagttctt
    17101 tttgattaat cttctgttgt aggtgttgat acttccgtaa cttctgcatc aacaggagtt
    17161 ccttcaataa tctcaacttc attaacagca agtggttcaa tattacggta acgagccata
    17221 ccagtacccg ctggaatgat tttaccgata ataacatttt ctttaagacc aagtaagtga
    17281 tctttcttac cacgaatagc agcatctgta agaacacgtg ttgtttcttg gaaggatgca
    17341 gctgataaga atgaattagt ttcaagggaa gccttagtaa tacccataag gacaggacgg
    17401 cttgtcgcag ggattccacc agaaataaca atatctttgt tagcatctgt aaagtctgaa
    17461 atatccataa gtgtacctgg aagaagatct gtatctcctg gatccatgac acgaacttta
    17521 cggagcattt gacgaaccat tacctcaacg tgtttatctc cgatttctac cccttggcta
    17581 cggtaaactt tttgtacttc agcaagtagg tacgtttcaa cagataaagt atcacgaact
    17641 tcaagaagac gttttggttg gatagatcct tcagttaagg ccgcaccacg agctacttca
    17701 tcaccaactt caactttcat acgtgctgta aatggaacaa cgtattctcc ctcacctgtc
    17761 tgacctttaa cgaatacttt cttagtacgt gttgaagagt cttcttcaat tgcaacgact
    17821 tcacccttaa cttcagtaat gactgcctca cctttagggt tacgtgcttc aaagatttct
    17881 tggatacgtg gaagaccttg tgtaatatca gtatttgaag caacaccacc ggtatggaag
    17941 gtacgcattg taagctgtgt ccctggttca ccgatagatt gagctgcaat agtaccaact
    18001 gcttcaccaa cctcaacagc atcacctgtt gccaagttaa taccgtaaca gtgacgacaa
    18061 acaccatggc gagtgttaca tgtaaatact gatcggatag ttacttcctc aacgcccgct
    18121 ttaacaactt tagcagccat atcttcagta attaaggtat ccgcaccaac gagaatttca
    18181 cctgtctcag gatgtttgat tgacttctta gtataacgtc ctatcaaacg ctcttcaagt
    18241 gtctcagtaa cctctttacc atctgtaatt gctgtgattg tcaaaccgcg gtctgttcca
    18301 cagtcatctt cacgaataat aacatcttgg gcaacgtcaa ccaaacgacg agtaaggtaa
    18361 cctgagtcgg ctgtcttaag ggccgtatca gtcatacctt tacgcgcacc gtgagttgag
    18421 aagaacattt ccaaaacact aagaccttca cggaagtttg ataagattgg caattccatg
    18481 atacgtccgt taggagcagc catcaaacca cgcataccgg caagttgtga gaagtttgag
    18541 atgttaccac gagctcctga gtccatcatc ataacgatag ggttctttgg atcttgtgtc
    18601 tcaataagac gtttttcaag tgcttcttta gcttcacgcc atgttgttgt aacagcaaca
    18661 taacgatctt cttcagtcat caaacctcga cggaaggctt tattgatatc ttcaactctg
    18721 tggtgagctg catcaataat ctcagcttta ttgtcaataa ctgggatatc agcaataccc
    18781 acagttaaac cagcaagtgt tgaatgataa tatccaaggt ccttcaaacg gtcaaggaaa
    18841 gcagaagttt ctgttgtacg gaaacgtttg aaagtttctg cgataatatt accaagattt
    18901 ttcttcttaa agggaatatt aatctctaag ttatcaataa ctgcttgaat atcctgacct
    18961 ggctctaaga agtatttatc tggagttttt tctgtcaagt ttgcattatt aggttcaatt
    19021 aagtacggta aatcttcagg catgatatcg ttgaagagaa tcttaccaac tgttgtaacc
    19081 atgattttat gtttttgctc ttctgtccaa ggcttattcg gcattgaatc aacagcgata
    19141 ccaacacgtg tatgtaaatg aacataacca ttttgatatg ccataactgc ttcgtcatga
    19201 tctttgaaaa tcatgccttc accttcacga ccagcatctt ccatagtcag gtagtaattt
    19261 cctaaaacca tgtcctgaga tggtgttacg actggcttac catctttagg gttaaggatg
    19321 tgctctgcag caagcatcaa aagacgagct tctgcttggg cttcttcaga caatggaacg
    19381 tggatggcca tttggtctcc atcaaagtca gcattgtagg cctcacatac aagtggatga
    19441 agacgaagcg ccttaccatc aataaggaca ggttcaaacg cttggatacc taatctgtga
    19501 agagtaggtg cacggtttag aagtactggg tgctctttaa taacttcttc taagatgtcc
    19561 caaatacgct cgtccccacg ttcaaccata cgtttagctg ctttaacgtt ccctgcgagg
    19621 tcacgagcaa cgatttcacg cattacaaaa ggtttgaaga gctcaatagc catttcacgt
    19681 ggtacaccac attggtacat tttaagtgtt ggaccaacag caataacgga acgtccagag
    19741 aagtcaacac gtttaccaag caagttttgg cggaaacgac cttgtttacc tttaagcata
    19801 tggcttaatg atttcaaagg acgactacct ggtccggtaa ttggacgacc acgacgacca
    19861 ttgtcgataa gcgcatcaac agcttcttgt aacatacgtt tttcattttg aacgatgatg
    19921 ccaggagcat ttaattccaa caaacgtgcc aaacgattgt tacggttaat aacacggcgg
    19981 taaaggtcat tcaagtctga tgccgcaaaa cgaccaccat ctaattgaac catcggacga
    20041 agatccggtg ggataaccgg taaaatgtta agaaccatcc attctggttt atttccagat
    20101 tttttaaagg catctaacac atccagacga cgaactgctt tcacacgttt ttgacctgtc
    20161 gctgatttca actcctcttt aagaactgcg atttctgcat ctaaatcaac gcgtttaaga
    20221 agatcttgaa tggcttcagc acccatttta gcaacaaacg aaccgtaacc gtactcttga
    20281 agtttttcac ggtattcacg ctcagtaagg agtgacttcg gttctaatgg tgtgtccatt
    20341 ggatcgataa caacataagc cgcaaaataa ataacttctt caagtgcacg aggactcata
    20401 tccaatgtga gtcccatacg cgatgggata cctttgaaat accagatatg agatactggt
    20461 gcttttaact cgatatgtcc catacgttcg cgacgaactt ttgcacgagt tacttcaacg
    20521 ccacaacggt cacaaatgat tcctttgtaa cgaatacgtt tgtattttcc acacgcacat
    20581 tcccaatctt ttgttggacc aaaaataact tcatcaaaaa gtccttcacg ttctggtttc
    20641 aatgtgcggt agttgattgt ttcaggtttt ttaacttcac cataagacca tgaacggacc
    20701 ttacttggtg atgctaatgt gatttgcata cttttaaaac gatttacgtc aaccactagt
    20761 tttacctttc ttcgatttat catcatacta gcccagctag gatttcaata aatcctagga
    20821 ctagcattgc atatcaattc tatattcgct tctagttata aactagataa atttaatctt
    20881 cttgaacgac ttcagatact tgttcagctt gtttttcttc agcttcttgt ttcacacgcg
    20941 ctttttcaag gtcatcaacg tgcatcacat catcatcttc accttcatca agatcacgga
    21001 gttcaacttc gttgtcatct tcatcaagga cacgcatatc aagaccaagt gattgcaatt
    21061 cttttacaag aacacggaat gattctggaa cacctggttt tggaattggc ttaccttttg
    21121 tgatagcttc ataagcttta agacgtcctg taacatcatc agatttgtat gttaaaattt
    21181 cttgaagaac atttgaagct ccataagctt caagcgccca aacctccatc tcaccaaaac
    21241 gttgtccacc aaattgagct ttacctccaa gtggttgttg agtaacaagt gagtatggtc
    21301 ctactgaacg ggcatgaagt ttatcatcaa ccatgtggtg aagtttgatc atgtacatga
    21361 caccaaccga cacacggtta tcgaatggtt caccagtacg accatcataa agaacagtct
    21421 tagcatctga atccatacct gcttcttgaa cagtttccca aaggtcttct gaagaagccc
    21481 catcaaagac tggtgttgca atatgaatac ctaaattacg agcagccata cctaaatgaa
    21541 gctccataac ttgtccgata ttcatacgtg atggcacccc aagtgggttc aacatgatat
    21601 caactggcgt tccatctggt aaataaggca tatcttcaac aggaacaata cgtgagacga
    21661 cacctttgtt tccgtgacga ccggccatct tatcaccgac tttgatttta cgtttttgag
    21721 cgatataaac gcggacaagc atattaacac ctgattgcaa ttcatcacca tttgcacgag
    21781 taaagatttt aacgtcacga actactccat cgccaccgtg aggtacacgt agtgaagtat
    21841 cacgaacttc acgtgattta tcaccaaaaa tggcatgcaa gagacgttct tcagcagata
    21901 agtccttttc acccttaggc gttaccttac caacaagaat gtcaccttct tttacctcag
    21961 caccaatgcg gataattccc atttcatcga gatcacgtag tgaatcttca ccaacatttg
    22021 gaatttcacg agtaatttct tcaggcccaa gctttgtatc acgcgtttca gattcgaatt
    22081 cttccaagtg aacagatgta tagacgtctt ctttcacaag gcgctcactc atgataacgg
    22141 catcctcgaa gttataacct tcccaagtca tataagcaac aactgggttt tgtccaagag
    22201 ccatttcacc attttccata gatggtccgt cggcgataaa atcacctttt tcaacgagat
    22261 caccaacttt aactaaggta cgttggttat aagcagtacc tgagtttgaa cggcggaatt
    22321 tttgaacatg ataaacatca agagaaccat cttcgcgacg cacttcaact ttttcagcat
    22381 ctgaaaaaat aacacgaccg tcatgtttag caatcacagc tgcacctgaa tcgtgggctg
    22441 cttgatactc cataccagta ccaacatatg gtgcttttgg atcaatcaat ggcacagcct
    22501 gacgttgcat gttggcaccc atcaaggcac ggttagagtc atcattttcc aagaaaggaa
    22561 tacatgctgt tgcgacagca actacttgct taggtgacac gtcaacaaag tcaacaatgc
    22621 ttgaaggaaa ctcttggtta ttaccttgat gacgacccat aacgatttct tctgcaaatg
    22681 taccgtcttc gttaagctta gagttggctt gtgcaactgt aaattcatct tcttcatcag
    22741 cagtcaacca aacaatttcg ttcgttaccg caccagtaga acgatctact ttacgatatg
    22801 gtgtttgaat aaatccatat ttattgaggt gtccaaatga agataagtta ttaatcaaac
    22861 caatgtttgg accttcaggt gtttcaattg gacacatgcg cccatagtgg gtatagtgca
    22921 cgtcacgaac ttcatatcca gcacggtcac gtgtcaaacc accaggtcct aaggcagaga
    22981 gacggcgttt gtgcgacaat tctgatagag ggttgtgttg gtccatgaat tgtgacagct
    23041 gtgatgaacc aaagaattct ttaacagcgg cagtcactgg acgaatgtta atgatttgtt
    23101 gaggtgtcaa gacttcattg tcttgtacag acatacgttc gcggacattg cgttccatac
    23161 gagcaagtcc gatacggaat tggttcgcaa gtaattcacc aactgcacga atacgacggt
    23221 tacctaaatg gtcaatgtca tctaccttac cgataccctc tgcaaggttt aagaagtatg
    23281 acatctctgc taaaatatct gcaggagtta aggcacgaac tttatcttca ggatttgagt
    23341 ttccaacaat agtaacaaca cggtctggat cagttggagc aacaactttg aatttttgaa
    23401 gaataactgg ttcagtaact acagcataat catttggtgt gtagacaaac ttgtttaagt
    23461 ctccatcgat atgctctgca attgaatcaa tgacatctcg tgtcataacg gttccagctt
    23521 caacaagaat ctctcctgtt tcaccatcaa ctaaattttc agcgattgtt tggttcaata
    23581 ggcgtgtttt aagattcagt tttttattaa tcttataacg accaacagcc gctaagtcat
    23641 aacgacgtgg atcaaagaaa cgtgcaacta aaagactacg tgagctgtct gcagtttttg
    23701 gttcacctgg acgaagacgt tcatagattt ctttaagtgc ttcatctgta cgagaatcac
    23761 ttggattttt atgaatgtct ttttcaatag tattgcgaac aagttcactg tcaccaaaga
    23821 tgtcaacaat ttcatcgtct ccagagaaac caagtgcacg gactagtgtt gtaaatggaa
    23881 ttttacgtgt gcggtcgata cgtgtataag caatatcttt tgcgtctgtc tcaagttcca
    23941 accaagctcc acggttaggg ataacagttg acccataacc aacttttcca tttttatcta
    24001 ccttatcatt aaagtaaaca ccaggagaac ggactaattg tgaaacaatg atacgttcac
    24061 caccattgat gatgaaagta cccatttcag tcataattgg aaaatcacca aagaaaactt
    24121 cttgagtttt aatttcacca gtttctttat taactaaacg gaaagtaaca aaaataggtg
    24181 ctgagtagct agcatcatgg atacgagctt cttccaaagt atatttaggt tcttttaatt
    24241 cataaccaac aaattcaaga tccatagtat ctgtaaagtt tgaaattgga agtacatcct
    24301 caaatacttc tttcaagcca gcatcaagaa aatcttggaa tgaatcagtt tgaatttcaa
    24361 ttaagtttgg taaatcaaga acttccttaa ttcttgaaaa gctacgacgt gtacggtgtt
    24421 ttccgtactg aacttcatgt cctgccaagt ttttagctcc tttttaaaga tagagtgact
    24481 aagtttttat ctcataaaac ttagtgagtt tatttcattt gctcgcctaa caagaacaag
    24541 cttatagatg tcgtttgtgg taattttcag aaagtttaaa tatatattac aataaccttt
    24601 tttcttaaaa ttaggcacaa aaagagcagc taaatctcac tttatcaagt tgatttaact
    24661 gctgtatatc taggtcggac aatatttcaa cctagacaga cgacaaatag ttacgtacat
    24721 tataacttat tttcaccaat aaatcaagtt ttattctctt gacttcaagg aagcttctag
    24781 aatttcaatt tgttcatcaa cacccttgag ttttagcata acacgatgat ttttaaacat
    24841 agcatactgg tgtttttcta attctttgaa agaagactat acaagaatta aatcatatta
    24901 tcttggaaaa aataaaagga gaacagatca ctctattctc ctcaaataaa actatcagta
    24961 tcgtaaacct gttatcgttt tccacccaaa gtagaccaag ctttttgata atcactatca
    25021 gttcccccaa tagcaaaacg ataggtcatc gttccaggtc cattttttgc ccaataactt
    25081 gttgtgcttt cacctccaac agtgattcga cgaccgttta ctgttactac acctggctgt
    25141 aaacctgtag acttaagaac tttagctttt ataacgctat catcaagtct aaacctttct
    25201 gacttaccaa aagtattgcc atctgcatta ttaatagcat taaccaagtg agccatataa
    25261 ttagcattat tattgtaacc tgtcaatttc gctaaagagg cattattatc atgtcctgcc
    25321 catccaccta aagtcacttt aggtgtagaa agcatcaacc aaacatcaga agttgagtta
    25381 gtagtacccg tttttccaat ccaatctacc ccagctaaac cactatttaa accttgtaag
    25441 cgatttttaa aggttgtagt ctttccggag ttaattggtc catgtaatag ctgctgtaag
    25501 atagtagctg ttgctttcga aaagacacgg acaggtttac tttcgtgatt ataaatcact
    25561 ttcccattac tatcctctat actttctatc atatactgct tatgataaac accaccattt
    25621 gcaatcattt gataaaggtt tgtttgctga gcaactgacg tatcaatacc accaccaagt
    25681 ggtaaacttt ctataccaaa gttttcaatt ggatagtcta atttctccat atagtttttt
    25741 acatcaacgc ctcgatctcg tagcatcttg taagtccaga aagctggaat gttccaagaa
    25801 atgtccaatg actcttgaag gttaaccata gcagtacctt cttcatccgc atgcataatc
    25861 ttttcgccac ttgaatatgt cgttggataa tttgaaagaa cactaccact tcctaacatc
    25921 ccttgatcaa tagcaattcc gtaaggtaat atcggtttaa tacttgaccc tggagagcgt
    25981 gccgtatcaa aagtatggtt attttgattc tcactatagt tacgaccacc aataaatcct
    26041 ataatagcac cactagaatt gtccgttagt acatttccca tttgaacctt gccagtacca
    26101 tcatctaaca accctccgta ttgagctgca gcatcctgca tggcttgata aacagattta
    26161 ttgatagtag ttttaatagt ataccctccc tgttgaatct cttcaatagc gcggtgtctg
    26221 tacgtcgctc tagtctcatc attttttaaa tcatgctctg aaacattatc ctttttaatg
    26281 aggtaattgt acatcacttt ttgagcttca gataatgccg agtagtaaag ataatcatga
    26341 tgatttgttg tcgccactgc aggtttgata aaatcttttt tgatgtcata atctttgtag
    26401 gatttgtact catctttggt taaagctctc gttctataca tattgtataa cacatttttt
    26461 tggcgcttta taccaaaact aagatcttta tctgatttca actgggcatc tgcagtataa
    26521 ggagaatata caataggact ttgcggtaaa cctgcaagga aagctgcctg aggaattgtt
    26581 aaatcttttg cggaaactcc aaaaatccct tgtgctgctt cttcaatacc tgcaatattc
    26641 tgtcctttgt tattgcgacc aaacggtgat acatttaaat aatctgataa aatcgaatcc
    26701 ttatccatgt aacgctctaa tgctagagca taaataattt cttttgattt gcgtttaaat
    26761 gacggatcat ctcccaaaat ttgctgcttt aataattgtt gtgtcagcgt tgaaccaccg
    26821 ctactctctc caaaccccag aacagatccc gctgcagcac gtagtacggc tttgggaact
    26881 acacctttat ggtcattgaa attttcgtcc tctgttgcaa tgatagcctt tttaatatta
    26941 tcagatatag catcctttgc cactggtgta cgttgtaaat cagtggcaat ctctgaaatt
    27001 tgtgatttat cagaataagt taacctagac accctagtaa gtgtattaac ttgtgtcact
    27061 aaactatttt tacttggtac tttaacagag tcaacttgac tagcaagata ccctactgca
    27121 agtcctactc cgagcataac aaatagtaaa ataataacat agatgaaatc tgataaaagc
    27181 tttacaattc ttaaaaaaat agaaccaaat tcaagtggtg tgtaatcacc taatttagaa
    27241 ctattcaact tcttattacc tttaaacatg aataatacct catcctacat tatatcagat
    27301 ttaatataac ttgcatatcc taaagagttt tgatatagtt gacattaata cacaacaagt
    27361 taataattag gagaaaaaga tgaatatttt tgatgaactc aaagaacgtg gactcgtttt
    27421 tcagacaact gacgaagatg ctttacgcaa agctcttgaa gaaggttctg tctcttacta
    27481 tactggctac gatcctacgg ctgacagcct tcatcttgga catttagttg ctattttaac
    27541 ctctcgtcgc ttacaacttg ctggtcacaa gccttatgca cttgtgggag gcgcaactgg
    27601 acttattggg gacccttcat ttaaagatgt tgagcgcagt ttacaaacaa agaaaactgt
    27661 agttagttgg ggtaataaaa tccgcggtca attatctaat tttctagagt ttgaaacagg
    27721 tgataacaaa gctgtattgg taaataatta tgattggttc agcaatatta gtttcattga
    27781 tttcttacga gatgttggta aatattttac tgtcaattat atgatgagta aagaatctgt
    27841 taaaaaacgt atcgaaacag ggatttccta tactgaattt gcttatcaaa ttatgcaagg
    27901 atatgacttt tacgaattaa ataagaacta taatgtcact ttgcaaattg gtggttctga
    27961 ccaatggggc aacatgactg ctggaaccga acttattaga cgaaaatcaa atggtgtatc
    28021 tcatgttatg actgttccat tgataacaga ttctacagga aaaaaattcg gaaaatctga
    28081 aggaaatgct gtttggcttg atgctgacaa aacctctcct tatgaaatgt accaattttg
    28141 gctcaatgtt atggatgctg atgccgttcg cttccttaag atttttacat tcctaagcct
    28201 taaagaaata gaagatatac gtattcaatt cgaggaagca cctcaccaac gcttagcaca
    28261 aaaaacacta gcacgtgaag ttgtaactct tgttcatggt gaaaaagctt ataaggaggc
    28321 cgttaatatc acggaacagc tatttgctgg caatataaaa ggattgtctg ttaaagagct
    28381 caaacaaggt ttacgtggag taccaaatta tcacgttcag acggaagata acctcaatat
    28441 catagacctt ctagtcactt caggagttgt caattctaaa cgtcaagctc gagaagatgt
    28501 ctccaatgga gctatttata tcaatggaga tcgtattcaa gatttagaat atactatctc
    28561 agaaaatgat aagcttgaaa atgaaattac agtcattcgt cgtggaaaga aaaaatactt
    28621 tgtatt
//
LOCUS       DATRNX010000011        27667 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRNX010000011 DATRNX010000000
VERSION     DATRNX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27667
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(370..1965)
                     /gene="cls"
                     /locus_tag="VBC81_000403"
     CDS             complement(370..1965)
                     /gene="cls"
                     /locus_tag="VBC81_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6911465.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQTLIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2084..3754)
                     /locus_tag="VBC81_000404"
     CDS             complement(2084..3754)
                     /locus_tag="VBC81_000404"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6911466.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            complement(3843..4862)
                     /locus_tag="VBC81_000405"
     CDS             complement(3843..4862)
                     /locus_tag="VBC81_000405"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6911467.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(4889..5767)
                     /locus_tag="VBC81_000406"
     CDS             complement(4889..5767)
                     /locus_tag="VBC81_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6911468.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5736..6554)
                     /locus_tag="VBC81_000407"
     CDS             complement(5736..6554)
                     /locus_tag="VBC81_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6911469.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6547..6879)
                     /locus_tag="VBC81_000408"
     CDS             complement(6547..6879)
                     /locus_tag="VBC81_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6911470.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(6908..7894)
                     /locus_tag="VBC81_000409"
     CDS             complement(6908..7894)
                     /locus_tag="VBC81_000409"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6911471.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRVIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(7891..9090)
                     /locus_tag="VBC81_000410"
     CDS             complement(7891..9090)
                     /locus_tag="VBC81_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6911472.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9083..9919)
                     /locus_tag="VBC81_000411"
     CDS             complement(9083..9919)
                     /locus_tag="VBC81_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6911473.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            10058..10756
                     /gene="coaB"
                     /locus_tag="VBC81_000412"
     CDS             10058..10756
                     /gene="coaB"
                     /locus_tag="VBC81_000412"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6911474.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            10753..11295
                     /gene="coaC"
                     /locus_tag="VBC81_000413"
     CDS             10753..11295
                     /gene="coaC"
                     /locus_tag="VBC81_000413"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6911475.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11341..11913
                     /locus_tag="VBC81_000414"
     CDS             11341..11913
                     /locus_tag="VBC81_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6911476.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12023..13741
                     /locus_tag="VBC81_000415"
     CDS             12023..13741
                     /locus_tag="VBC81_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6911477.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(13805..14002)
                     /locus_tag="VBC81_000416"
     CDS             complement(13805..14002)
                     /locus_tag="VBC81_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911478.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(14016..15749)
                     /locus_tag="VBC81_000417"
     CDS             complement(14016..15749)
                     /locus_tag="VBC81_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911479.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLSLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(15750..17471)
                     /locus_tag="VBC81_000418"
     CDS             complement(15750..17471)
                     /locus_tag="VBC81_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911480.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(17483..18085)
                     /locus_tag="VBC81_000419"
     CDS             complement(17483..18085)
                     /locus_tag="VBC81_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6911481.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(18087..19064)
                     /locus_tag="VBC81_000420"
     CDS             complement(18087..19064)
                     /locus_tag="VBC81_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6911482.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEHFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(19069..20325)
                     /locus_tag="VBC81_000421"
     CDS             complement(19069..20325)
                     /locus_tag="VBC81_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6911483.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(20417..21013)
                     /locus_tag="VBC81_000422"
     CDS             complement(20417..21013)
                     /locus_tag="VBC81_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6911484.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(21006..21836)
                     /gene="prmC"
                     /locus_tag="VBC81_000423"
     CDS             complement(21006..21836)
                     /gene="prmC"
                     /locus_tag="VBC81_000423"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6911485.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(21836..22915)
                     /gene="prfA"
                     /locus_tag="VBC81_000424"
     CDS             complement(21836..22915)
                     /gene="prfA"
                     /locus_tag="VBC81_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6911486.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(22950..23519)
                     /locus_tag="VBC81_000425"
     CDS             complement(22950..23519)
                     /locus_tag="VBC81_000425"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6911487.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            23657..23839
                     /locus_tag="VBC81_000426"
     CDS             23657..23839
                     /locus_tag="VBC81_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6911488.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            23988..24926
                     /locus_tag="VBC81_000427"
     CDS             23988..24926
                     /locus_tag="VBC81_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6911489.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            24944..25546
                     /locus_tag="VBC81_000428"
     CDS             24944..25546
                     /locus_tag="VBC81_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6911490.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            25570..26805
                     /locus_tag="VBC81_000429"
     CDS             25570..26805
                     /locus_tag="VBC81_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6911491.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            26904..>27667
                     /locus_tag="VBC81_000430"
     CDS             26904..>27667
                     /locus_tag="VBC81_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6911492.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLN"
BASE COUNT         8944 a         5291 c         4382 g         9050 t
ORIGIN      
        1 tagcatattc aattaaagtt aagtaaaaac ataattttta gaaagaaatt gtgacttatt
       61 tatgcaaaaa aaagaaagaa ccaaactggt tctttctttt ttgcttttca gctacttacc
      121 acagtttgta atgaattaca agtagtagta agctaagggc accttttcaa gtgtgataag
      181 aaggttcaca caacttatca agatccgctc ctgcgtttat aatctccctg cgcaatagtg
      241 gtaaaaccac atatcgactc atcgcataac agtagtttaa cataatttag ttaaaatgca
      301 acctttactt tttaaatata actagattct tcagtttact tttaatatta aattaaaaat
      361 aaattatttc taaagcaggg gagcaatagt ttttgtcact atgccaatta ctttttggaa
      421 ttttggaata ttatttatat ctttcaagct aactaacctt gatttattta acgtatccat
      481 ataatctcga taaatgtctt ggattgcatc taccttataa agataaacag cacattcaaa
      541 atggtgatac aggctacggt aatcaagatt tatcgtacct acaacagcct ttgtattatc
      601 actaaggaat atttttgaat gaacaaatcc aggggtgtat tcatatattt taacacctga
      661 ttttattaat gtttgatagt aagttttagc aagagcataa gggattggct tatctgggat
      721 acccggcata ataatacgta catctacacc acgttccgca gcaaactgaa tggcatgttc
      781 caattcactg tctaatatta aataaggtgt catgatgtaa acatattctc tagcatgatt
      841 aagaatatct atataaacat tttcccctac tttatcagtg tctaaaggag aatctccata
      901 aggaataaca tagccttcat tttcaactaa atcatcgtgt acaccgaggt aaggcgctac
      961 taacattttt tcctcagtaa tggaccacat ctgtaagaaa agtaggagga aactatccac
     1021 tgctttcccc tttaacataa tacctgaatc tttccaatgc ccaaataact caatgtgatt
     1081 aatatactca tcggctaagt ttacaccacc agtcatgcct actactccat caataacaac
     1141 aatttttcga tggtcacgat agttataata agttgaaata aaaggagaaa tcggagaaaa
     1201 tgcttttgct tttattccta ttttctcaag tcgtttcgta taatcaaagg ataattttgt
     1261 aatttctatc ataccatcat ataagatacg gacttcaaca ccttcttcca ccttttgttc
     1321 taaaatacta agtatttctc cccacattat tccttcgtca atgataaaga attctaaaaa
     1381 gatatacttc ttagctttta gtagttcttc tttcattttt tcaaagaatg cttctcccgt
     1441 tggtagaaat gtcacatcag tatttcgata aactgggaaa tttccatgtg ctttttcaaa
     1501 atattgaacc aaatgatatg ttgtggatgt actcgactta agctgattca aagtttcagg
     1561 atcatcctct agataaatct gactctcatc aatcaatgtc tccaatcttt gtttcaaacc
     1621 acgataaccc cagtcgaatt tggtgtacat taggaacatc gcccctagta aaggggcaat
     1681 cattaccaat attaaccaag ttactcggga caaagcatcc atttcactat taactaaata
     1741 taagaccgct ccaattgtta agatatgctc aacggttgct aaccaaacac gatactgctc
     1801 tagccatgag taagaggcta gtaaaaacaa taattgtaaa atcaataaaa tagcgataac
     1861 ggttgttcga ctaaatatac cccttagtaa acttttctta ctcttattaa gtaaacgaac
     1921 aattttgact ttattactga taagtttttc ccccctccaa atcaatcatt ttattatagc
     1981 atactgtcac ttgaatagca ttctattatt ctaaattatc tctagttaaa atctaatccc
     2041 caataaaaag aggctagcat cacaagccct acctctttat tatttaaaat aatccaacgg
     2101 ctgtaccgtc ttctaaaaca tccatattga gagctgcagg ttttttaggt aaaccaggca
     2161 ttgtcaatac gtctccagta agagcaacta taaaaccagc tcctgttttt ggaacaaatt
     2221 cacgaacagt tatatcaaag tcagttggag caccaaataa atttggatta tcagaaaagc
     2281 tatattgtgt ctttgccata caaataggca tcttgtccca gccattgtca ctaaactctt
     2341 ttaattgtat ttgtgcctta ggtccaaaat gaactttatt accaccatat attttagtaa
     2401 caattttttt aattttttct tcaatagtgt cttcatcatt atataagcgt ttgtaattgg
     2461 atgattgtgt ttcaatcaca ttagcaacag tctgtgcaag ttcaaggcca ccgtctgctc
     2521 catcttccca cacacttgct aattcaactg ccacatcaat attactacat aaggttttaa
     2581 gagttgcaat ttcactttct gtatctgcag taaactgatt gatagctaca actaccggaa
     2641 caccatattg acgcatattg ttaacgtgac gctcgagatt tgtaaaacct cttttaactg
     2701 cttcgacgtt ctcttgtgag agatcttctt ttgaaacacc tccatgcatt ttcaatgcac
     2761 gcaatgtagc aacaataaca atcgcatcgg gagaagtcgg aaggtttggc gtttttatat
     2821 caagaaactt ttcagcgccc aagtctgctc caaaaccagc ttcagtaaca acgtaatctg
     2881 ctaagcgtag agctgttgaa gtcgctaaga cagaattaca tccatgtgca atattagcaa
     2941 atggtccccc atggacaaga gccggtgtcc cataaatggt ttgaaccaaa ttaggtttga
     3001 tagcatcttt taggataagt gtgagagctc cttcaatctt caaatcttta acatagattg
     3061 gtttacggtc ccttgagtaa gccacaacaa tatttgacag gcgtttcttt aaatctgata
     3121 ggtcagtagc taaacataga atagccatta tttcagaagc aacagtaata tcaaagccat
     3181 cttcacgtgg tatcccattg acagggcttc ctaagccaac gatcacctga cgaagagcac
     3241 gatcattaag atcaactaca cgcttccaaa tcactcgacg ttggtcaata tctaattcat
     3301 tgccttgatg aatatgatta tcaagtagag ctgataaagc attattagca gttgtgattg
     3361 cgtgcatatc accagtaaaa tgcaaattaa tatcttccat tggtagtact tgtgcgtatc
     3421 cacctccagc agctcctcct ttaataccca ttacaggacc taaagaaggt tcgcgaagcg
     3481 caatcatcgt ttttttacca attttattta aagcatcagc taatccaatg ctcatagtgg
     3541 attttccctc acctgctggt gtcggattaa tagcagttac taaaattaac tttcctactt
     3601 tttgagatct tacagcttca atcttatcaa aagataattt tgctttgtat ttaccataca
     3661 attctatatc atcaaaacca attcctactt gctcaacaat ctcagcaata ggctttaggg
     3721 cgacgctttg cgctatttca atatctgttt tcatgatatt ctccttctaa ataataaaaa
     3781 tagtatagca atattcccta taaaaacata tttttttgca aaaaaaattt aaaatgttcg
     3841 tcttatgaaa ataaaactgc ttttatttct tctggactag tctgaccaaa ataatcttct
     3901 aatgataaag tacttaattt ttccaagact aattcttctt tcatagggca accaatgaga
     3961 aggttttcaa tatctgaaat atctttagaa ctgaaaaagt caccataaat acggcaattc
     4021 gtaataatac ctttctcaat ttctaagtga aaatcgtaag ttcctgacgg aaaacgagca
     4081 tctctgtgat acgaatactg tggtgagtta ccataattcc aatcccaatt cttatatttt
     4141 tcatcagcta gagcgtcaat cgcagcccaa tctttttcgg ttagatgata gcgttttgcc
     4201 tgatcaatat gatcaatatg taataattga caaaccatta aatctttaaa ttgatcggtt
     4261 gtgatatgac gataatcttc tgacaagtgg ctacgaatat ctcctactcg acttcggaca
     4321 gattttatcc ctttagattc aatttttttc cgattaggat ttaacacttt ttccatagca
     4381 tcaaaatcaa catctaataa aagagagtaa ccaccgtaaa tacgaccgtt tactatcgtc
     4441 attgctgcac ctgatacttt tttaccatca ataactaagt catttcgtcc cctcatttca
     4501 acactagaag cacctaaagt ttttagggcc ttaatagctg gctcataagc tcgtttgaaa
     4561 tcaccaaatt gtccatgatc tttcattaga tagcaaatat ttacagcacc tgagtcaacg
     4621 taaacagctc ctccaccagt atctcgacga acaacaggaa tatcatgttc tttcaaatat
     4681 tcttgatttg tctctataac tgcattctga aattttccga tttcaacttt aggatcacaa
     4741 tagtatggaa ataaaatatc atcatctaaa aaaacatttt gtaaaatata aacttgcatg
     4801 gcaagtgcaa tcgcaccgtc tgtgatacgc ttaccatttc taattggttc aataagatac
     4861 atttaggtct ccttttatag caatttagtt atccaataat ttcttttttc gcttcagcaa
     4921 ttaattcagc gatatgctgt gtcagttgca cactccttga gcgaatcttt tgaggaatgc
     4981 gataatgctt gtgattaaga gtaacaaata aagagttttc atttaaagag acgaatcgcc
     5041 aaaacggatg cttaataaat tgtggcgtgg tatgtccaac tccaatctct agataaagaa
     5101 ctttgtcatt ttgatgttgt gataagaaat tttcataacg ttgtcgttga gcatgaaagt
     5161 cagcatcttc caccatacct ttttcttcgt tacgtttatt aatttcaaac ggttgatcac
     5221 actttgggca tttggggatt aagttactag gaatcttcat atccttttgt tgtgcaatca
     5281 tttgacgaat tgcctgatca tttcgataag tttgttgatg acaatgttga ctacactgcc
     5341 ataagccata ttcaccttga atatgaaaaa ctttttctaa attataatca gccacagcga
     5401 aggcattatc agcattagtt gtaataatat gatattcttt tttcgctaaa atatccttta
     5461 aatcaagata agcttgtcct accggttgat ctaaataatt aagtgccaca aaacgacttt
     5521 ggaaggccca atattcttcc caatcttcaa agtcataaag acttgcttgc aacatatcta
     5581 atagttgata tttagcaata aaatccggaa aagcttcttc aaatctgggg ccaatatagg
     5641 taaatccatc cgctgcagac atgcctgcac caataccaac tacaatagca tctgattctt
     5701 ctattaactg acttagtatt tctgattgag aatgattagt tttttctaga gtcttccaca
     5761 ctgtcatcta tttcctcttt taataataat ttttgataaa tctcttggtc tttaggtgtg
     5821 aatgtattga aaataatata gagtttgtgc tgattttcgc gttgccactc taatatctct
     5881 tgaacagcca attcagcggc tctttgatta ggaaaaccaa attctcccgt tgatatacaa
     5941 gggaagacaa ttgttttaag attagcttga gctgccaatt gtaagcagga tttataagaa
     6001 gactttaata aatcctctct tatacgagat ggcttttgat caacttttac ataaggtccg
     6061 actgtatgaa taacatactt acaaggaaga tgataggatt cggtaagttt agcttgtcct
     6121 acggcttcca ttcgtccttg ttgtgtcatc aattgatgac atgctaaacg taatcgtgaa
     6181 cctgcaaacg tatgaatctg attgtcaata caatggtgat taggaataaa acaacctaac
     6241 aacttactat ttgctgcatt aacaatagca tccacttgta ataagcaaat atccccatgg
     6301 taggtaaata catttgaata taccgtttga tgacagacgt ctatcgtttt tactttttga
     6361 gtattccacc aatgtgacaa atacctatct tctaaagaca ggtactcttg actaatgttt
     6421 tgaggatctt gttgattaac tagaccacgc caaaccgttt gcaaatcggt ggtcctcaag
     6481 tcatcaacgt ctgttaattt ttctgactga agatactcaa tcatggctag caatagttgt
     6541 ttttgattag gcattttcta aagcttcaaa agcagcataa tcaacatctg ttaacacaac
     6601 caaccaattt tcgtcagctt tttcagaatt taacaaagtt ggttcttgac ttgcagctgt
     6661 attaacttta acaactttac ctgtaagagg agataaaatt gccataaccg tctttgaagc
     6721 ttcaatattt aaaataacat catcaacttc taaatttgta tcatctgtga attccacata
     6781 tccaacagtt cccaagtcat cttgaagttc gggagttaag ctgatagtat aaagttcttc
     6841 atttttttca attaataagt agttcgcaat ttttttcatg agtaatttcc tttctgagaa
     6901 aacgtgatta atataactgt gataataatt ttaaggattt gattcgttga tcaagtcctg
     6961 gaacaagagg aatcaaaagt agttcttctg cttgactagc attaactaaa gcgtcaaggc
     7021 tttctttaac ttgttttgga tcacctacaa tcacacggtg gcgatttgat ttaatttttg
     7081 ctttttgttc aggagtcaat tggtaatgat tagcttcttc aatagtagga aatgttgcaa
     7141 attcattgaa gtctttattt cctagcatcc agatatctaa agtctttgct atgttttcag
     7201 cttcttctga agtatcagct ataactacaa atggagctac catcaaatta ggagacttat
     7261 taggataata ttcttcaaac tgatgataat aaagagatga aagcttttta gcttctgtca
     7321 atgggtcttt gtccataaaa ggaaaaacac cgaaggtaaa gcctaagcca agtttagccg
     7381 ctaaataagc tgatttttga ccactcccca acacatataa gtctgggaag ctagaaagag
     7441 tcggttgaac taatggttcc ttactggatg attcatcaat aagccatgac ttcaattcct
     7501 ccagtacctc ttcgtaatca tgtgctttat gtaagctacg aagtgcattt gaaactttaa
     7561 ctgtccctag tgaatttcct aaaccaatac ttactcgatt cggatgacat gtctctaatg
     7621 tcttgagagt ctccgcgagt ttaaaactac tatagtgcag aggcattata cctccagagc
     7681 caattttgat agatttagtc tggttagcca aatgcattat cattaattct ggattgctaa
     7741 tactgaatgc cttaacaccg tgatgttcag ccacccaaaa ttgatgatag cctagttgct
     7801 ccgcctcttg tgataaaaga atcgtttctt gtattgcatc acttgcagtt tttccataat
     7861 caataagccc ataatcaagg acacttaatt tcatctttcc tccctataat tgcttcgaag
     7921 ttctctaaaa acttctcgag cttcctttgg taaagattct ccataatcca tcaaaggaac
     7981 aatatcttta aaggaggctt ttggaatcgc caaaccttct aaatcattca ctttaatcct
     8041 taaattaatc tgatcagaac gcttagcttt tattttttgt aaaaattccg gatcaactac
     8101 taagggcgtg gaagctccta tcatatgagc atgttgtaag gcttcaaaca cctttgaagc
     8161 actattaata cctccagttg ccataattgg aactctatca ccaaaatgtt caaatataac
     8221 ttgattaacc agctgccctt tgtaaactcc ctcagaacgt atagtatttc taaagacatc
     8281 atgcccccag cttgcaatgg caagataatc tacctgagca atcgctaaaa ttttttcaag
     8341 aaattccata aactcttcaa tgctatatcc aatttgtgaa ccgcgagttt cttcaggcgt
     8401 cgctctaaaa ccaaggataa aatctgactc cgcttcttct ctgataactt tttgtaccgc
     8461 tttaaaaact tctaagccta aacgacagcg attagtcaaa gtttgaggac catactcatc
     8521 ttttctttga tttgagaagg ttgagaaaaa agtttgaatt agtaatcgtt gtgcagaaga
     8581 tatttcaaca ccatcaaatc cagcttgtat agctcgtctt gtagcctgga catattcatc
     8641 gataatcctt agtatatctt tgtgtgttag ttctttaacc tgatgtgggt atggcgattg
     8701 caattgcata gggctgggac catatacata tccgtgtctt gccaaagtgt gacttgagaa
     8761 acgtccagca tgcgtcagct gcaaaacagc tttcgctccc ttagacttca ttgctttagc
     8821 caatttagtc aaaccaggaa tgtcctcatc ctttgaaacg ctaaaaccat attcaaataa
     8881 ctgaccatat tctgtgatat atgctgctcc agtaatttgt aatggagcgg atttagcacg
     8941 acgaacagca tatgctatgt cgtcatcagt tacaaaacct tctgatgtag aagaattagt
     9001 taccataggt gatagaacaa aacgattttc taagctcaat ccattgggaa gtgttaatgg
     9061 acgaaataaa ttaccttgga cattattcat ctctgctcac catcccctta ttagccatag
     9121 acttagcctg aaatctatca aactctgcaa ctaaatcggg tctaatcatc agtaaacgat
     9181 cattaaaacc tacttgttta aggctaatca acatacgatc aataacatct tctactgtca
     9241 taggacaatc taatagatca gatagattag ccataatttc aggatctaca tttggataag
     9301 caattggact acccgtatca cctagaccaa tcttataaaa atctgaaatc atttgactcc
     9361 gccctttttg atcgccacaa acgctaaggt aaattgatac cgcaatacca ttctttatac
     9421 ggcgctgagc caagccagca aattttttgc catttatact aagatcaaac ttaccaggac
     9481 aataggaggt ctctacttca aagtgctcaa taggttgata aaaatccgaa aatatacttc
     9541 taataaaatc gaccattata agatacccat ctgagataga caattttctc tcaaaaacat
     9601 ctggaataac caatgaaaaa tttaaaattc cttcatcagc tacaactgcg agacctccaa
     9661 aattccgaac aactggttca taaccacgac tgatgatttc ttttttagct aattctaaat
     9721 gtggtaattc tcgatctaac atccccagaa tgactgtctt agtcatcggc caaatatgca
     9781 aaataatttc ttgatttgag cgattaatct cccttaaaaa tacttctgtc catataaaag
     9841 gtttttccgc gtcttgagca tctgtaacat agtctttaaa aatagataca ggtaactgcg
     9901 ctagatcttg ccactccaat aaatctgacc tcctttctct agaaaactct gcaagattta
     9961 ttttatcgca atcgcttgaa attttccagt aagtttccct caaattccat tactatcaag
    10021 aagaaatata tcttttattt acgaaaagca tttttttatg tacaatatgg ctatgaaaat
    10081 tttgattaca tcaggtggaa ctaccgagaa aattgatacc gttaggagta tcacgaatca
    10141 cgcaacagga gctttaggaa aaataattgc agaaaaatat ttacgagaag gacatcaggt
    10201 gacactcgtg actactaaaa atgcagtcaa accagaatct gctaccaact tatccatatt
    10261 tgagattgag gacgttgact cattaataaa aaccttgaag ccattagtga aagagcatga
    10321 tattttaatt catagcatgg cagtgtctga ctataccccc gtctacatgg ctgatttcga
    10381 aaaggttaaa tcaagcgatg atttagatac ttttttgcgc aaagataacc acgaagggaa
    10441 aatatcatct gaatcagaat atcaagtcct ttttctcaaa aaaacaccaa aagttatcag
    10501 ccttgtgaaa aaatggaatc ctcaaattac tctagtaggt tttaaactac ttgtgaacgt
    10561 tactaaagaa aaccttttta aagttgctcg tcatagctta atcaaaaata aagctacgtt
    10621 tattttagct aatgacctca ttgatattac ctcaaaacat cacatagcct acctacttga
    10681 tcatgataat gtgtataaag ctaccaccaa agaagatatt gctcaattaa tttacgagaa
    10741 ggtaaaaaaa atatgattaa acgtattaca ctagccgtca caggttcaat ttcggcctat
    10801 aaagcagcag accttacaag tcaattaact aaaattggct atgatgttca tatcataatg
    10861 acacaagctg ccacacaatt tattacaccg ttaactctgc aagtattatc aaaaaatcct
    10921 attcatttag atgttatgga tgagcataac ccaaaaatca tcaaccatat cgaattagca
    10981 aaacgcacag atttatttat cgttgcacct gcctctgcta atactattgc tcatctagca
    11041 tatggcttcg ctgataacat cgtaacaagc gttgctcttg ctatgcccga tgaaactcca
    11101 aaacttatcg caccagcaat gaatacaaag atgtatcata atacaatcac ccaacgtaac
    11161 attgatattt tgaaaaaaat aggttatcag gaaattgaac ctcgtattag tttgcttgcc
    11221 tgtggtgata ccggtcaagg agccctagct gatatctcta ctatacttaa atgtatacaa
    11281 gaagttgcaa attaggctgt aaatttgtat actatttcta gtttaattta aggagttata
    11341 atgaaaaaga aaaacaaatc ctctaacatt gctataattg caatcttttt tgctattatg
    11401 cttgtcattc attttttgtc atcatttatt tttagttttt ggttagtccc tattaaacct
    11461 actttgatgc atatcccagt tattattgca tctatagcct atggacctcg tattggtgca
    11521 actctaggcg ccttaatggg ggggatcagc gtagctaaca gcagcattgt tctattacca
    11581 acgagttacc tcttctcacc ttttgttgaa aatggtaatt tttattcgct aattattgca
    11641 cttgtaccac gtattctaat cgggattatt ccttatttcg tttacaaatt actacacaac
    11701 cgctttggtt tggctatctc aggtgctata ggctctctaa caaacacagt atttgtttta
    11761 tctggaattt ttatcttttt ttcaagtact tataatggga atatcaagct aatgctcgct
    11821 gggattattt catctaattc attagctgag atggtcattg cagctatcat tgtatatcta
    11881 actgttcctc gtattctcaa tattaaacat taaaaactgg ccaggaagaa atcttcctgg
    11941 cttttcttac ttatatttta tagtcatatt gaaaaaaaca tgttataatg aaagcgttta
    12001 taaatattga aggagtattt caatgactta cacagaaaat tttcaaaaat ggttagattt
    12061 tgagcagctt cctgactatc ttcgtcaaga attactatca atggatgaaa aaacaaaaga
    12121 agatgctttt tacacaaact tagaatttgg tactgctggt atgcgtggtt atattggtgc
    12181 tggtacaaat cgtattaata tttatgttgt ccgtcaagca accgaaggat tggcaaaatt
    12241 aatcgaaact aagggagagg aagctaaaaa acgtggtgta gccattgctt atgattcacg
    12301 ccacttctct ccagaatttg cttttgagtc tgcacaggtc ttggcacagc atggaattaa
    12361 atcttacgta tttgaagctc ttcgtccaac accagaactc tcttttgctg ttcgccattt
    12421 aaatgcctat gctggtatta tggttactgc aagccacaat ccagcacctt tcaatggtta
    12481 taaagtctat ggacaagacg gaggtcaact accaccagct gatgctgatg cactaacaga
    12541 ctttatccgt gctattgaaa atccatttgc agttgaactt gctgatttag atgaaagcaa
    12601 atcatcgggc cttattcagg ttatcggtga agatgttgat attgaatatc ttagagaggt
    12661 taaagatgtt aatatcaatc aagatttaat caataacttt ggaaaagaca tgaaaattgt
    12721 ctatactcct ctccacggaa ctggtgagat gttgacacgc cgtgcccttg ctcaagccgg
    12781 ttttgaatct gttgttgtag tagaatcaca agctaaggca gatccagact tttctactgt
    12841 taaatcgcct aaccctgaaa gccaagctgc ctttgctctt gctgaagaac tcggacgaga
    12901 agttaatgca gatgtcttag tagctactga tcctgatgca gaccgcctag gcgttgaaat
    12961 tcgccaacca gatggttctt ataaaaacct ttctggaaat caaattggag cgattattgc
    13021 gaaatacatc ctggaagctc ataaaacagc aggaacactt ccagaaaatg ctgctctcgc
    13081 taaatcaatc gtatcaacag aattagtaac aaaaattgcc gaaagttatg gcgctacaat
    13141 gtttaacgtt ttaactggat tcaaattcat cgctgagaaa attcaagagt ttgaagaaaa
    13201 acataaccac acctatatgt ttggttttga agagagcttc ggatatctta ttaaaccatt
    13261 tgtccgtgat aaagacgcta ttcaagcagt tcttttggtt gctgagattg cagcttatta
    13321 ccgttcgcgt ggtttaacac ttgcagatgg tattgatgaa atttacaaag aatatggtta
    13381 ttttgctgaa aaaacgattt cagtaacact ttcaggagtg gatggagctg ctgaaattaa
    13441 gaagattatg gacaaattcc gtgaaaatgg tccaaaacaa tttaacaata ctgatattgt
    13501 attattagaa gatttccaaa aacaaatcgc tactaaaaat gatggaacaa tatctaactt
    13561 aacaactcct ccaagtaatg ttttgaagta cacgttagct gatgattcat ggattgcagt
    13621 tcgcccttca ggaactgaac caaaaatcaa gttctatatt gctacggtcg gtaatgattt
    13681 agcagatgca gaaacaaaaa tcgccaatat tgaaaaagag attacaacat ttgtaaatta
    13741 ataaaaaaag ctaggaactt actcctagct ttttttatat ctatctaacg atgttctatt
    13801 attttcaatc atgaaatata actgcaatta acttcacgat caaataatag agcagactta
    13861 caattaacca cgttatccca ataactgcaa ctaaacctat taaaacagat aataccgtat
    13921 tcttagttgc tagttggtta ctgataaacc atgaaactgc agaaataaat atcagtaata
    13981 atagttgaag cctttttttc atataattcc tcttactact tttgtgcatt ttgcatttga
    14041 taataaatac ctttttgagc tattaattca gagtggttac ccacttctac aatttcacca
    14101 gataccataa ctaaaataat atctgctgtt tgaatggtag ataagcgatg tgcaataata
    14161 aagcttgttc tccccttcat caacatttgg aatgcttctt gaacaagaac ttcagtacga
    14221 gtatctatag acgatgtcgc ctcatctaat atcaatattc taggtaattt aagaaaaata
    14281 cgagcaattg tcaatagctg acattgcccc tgtgataacg aatctccagc atcttctaaa
    14341 tacgtatcat atccattcgg taattgtttg ataaagaaat ctgcattagc tgcttttgca
    14401 gcctctatca cttcttcacg gctagcttta ggattggcat atgcaatgtt atcgtgaatc
    14461 gtagcacttt ttaaccacgt ttcttgtaat accattccaa tttcttgcct taattgtgat
    14521 ggctcataat cagtaatagg cttaccatct aaaaggatat ttcccccgtc aacttcatag
    14581 aaacgcatta ataaattgat taaagtagac ttcccagcac ctgttggtcc tacaatagcg
    14641 accttagctc ctgctggaat gtgtaaatta ataccattca atagtggttt tgatttatta
    14701 taaccaaaaa taacgttttt aaaatcgatt tgacctttga ccgttgatga atcaagtttt
    14761 tctgtacctg ttatatttgg tgacgattct tctaaaatag agtaaaggcg ttctgcacaa
    14821 gccagagcac tttgcatctc agacaggacg gaagagatgt cattaaatgg cttagtatat
    14881 tgattgacat aatttaagaa agtgattaat tgaccaacac taaaagcacc tgacataata
    14941 cgaagggcac caatccctgc taagaagcca taaatcaaac tattgataaa tcgggttgat
    15001 ggattgacag ttgaagaata aaaaattgct gattgagaaa agttagcata ctcttgatta
    15061 atagtcctaa aatgatcact actttcttct tgcgcactga atgcttgaat aaggctttct
    15121 tgactcacca tctcttcaat gaattgagtt tgtcgtcctc gtgatgccgt ttgattttga
    15181 tataagtgat aactcttttt agcaataaag cgggctagaa agagtgataa aggcgtcaga
    15241 aataatacca aaaaaagcat taataaatca atctttgcca tactaaaaat agtaactaga
    15301 atagttaata aaccaacaaa gaactggtta aaaaccatta ataaaccatt tgataattgc
    15361 tccgtatcgg tagtcacacg actaatcaag tcacctatgc cacgtttatc aaggtaagct
    15421 ataggtaata gactcaactt ttccattaca gcctttctta aagaagcaac ataatgaaaa
    15481 attaagcggt tatagagcag aggattaatc cattgaataa tagtatttgc taaaataact
    15541 gcaaccatct tccacattat aggtaacaat aatatcatag aatgaggcga taatacaacg
    15601 tctaccgcct gtccaattag aacaggcaaa taaacagtga ggcaaacttg tactaccgtc
    15661 cctaataatg ccattcctac taagcttttt ttagaaagaa gatcttgaac aagctttttt
    15721 tgtatctttt taggatgact tgctttcatt tactcctcct ccttaaaatt ttgtgaatgg
    15781 taaatggact tataaacttt gttatgagct aataaagatt gatgattatc aaaaccaact
    15841 tgtttgccct tatcaaggag taagattcta tctgcatttt gtatactatt aattctctgt
    15901 gatacaataa taagattagt ttggttgaaa tgtttaatta tagctttaaa taaacgagct
    15961 tctgttaaat aatccaatgc agatgttgca tcgtctaaaa tcaaaaatgg aatcttatct
    16021 tgaactaaag ctcttgcaat cgtcaacctt tgcctttgac caccagagaa attcctacca
    16081 aaactctcta caggggcatc aagttgacca tctttttctt ttacaaaatc actagcttgt
    16141 gctatttcta aagcagtcca aagtttttcc tcactaactt taccaagtcc caaagaaagg
    16201 ttagaacgaa tagttccttt aaacaactga gcattttgag gaacaactct taccaggcta
    16261 cgccaatttg aaattgtatc tggacttttt ccctgatgat atatatcaat atccccttct
    16321 tgcactttat aaatatgaag cagtaaatta accaaggttg atttcccaga gccagttcca
    16381 ccaattatcc ctaaagtttc tcctacattc atagaaaaag taatatcaga taaagctttt
    16441 tcagaggagt ttggataaga aaatgccatt tcttgcactt ctaaagcctt attcgaatat
    16501 tttggctcaa gtttgtcatc aattatttca gacggtcttt caaaaacggc tataattcgc
    16561 ttagcactga tataactttg gttcagtgat gtcaccaaca ttgtcatttt taacaattca
    16621 acaagaattt gcaataagta attaattaaa gcaaccaaca ttccttgtga taaaagatga
    16681 ttagctatat ttaaattacc acgccaaata atcacaacca aagtgatatt aacaactaga
    16741 aatgttagag gcgttactaa actagaaagc ctacctgctt ttaattgtaa attcgtatag
    16801 tgataattga tatcattaaa tgcttcactc tctctatcta cttgattaaa ggcacggata
    16861 actcgtacac cttgaagttg ttgcctagtc aactttacca aataatcggt tgaagttctg
    16921 attttaaggt aaataggatt taatagtcga gacattacaa aaacaatgat aaataacgtg
    16981 acaaccatta tcaagaacca aatagtcaat gagggactaa tggaaaaagc cataattatg
    17041 gcaccaaaaa caataatcgg agccctcaaa aataaacgta aaaattgatt taaaccagtt
    17101 tggatgtgaa atgtatcagc agtcaaacga gtaatcaaac tagcagttcc caattcatct
    17161 ctgtcttttt tacccagtga catgactttt tgatagagat cctctgtcaa ttgtctcgta
    17221 taaccaactg cggcttttga agaataatat tgagcagtta tcgctactac aacacccacc
    17281 gcagccaaaa agaaaatcaa tccaatttgc aacagtaaac tacttctatc ccctcgtgga
    17341 atggtctcgt caatcatttt tgcaataacg attggtacca aaagttcaaa agaggcttct
    17401 aataatttga aaaggggtcc aaagatagtt tctttgatat accccttaaa ataataagtt
    17461 agtcttttca tacttaccct tcttaaaaca actccattat ttttatcaaa taaaggttga
    17521 attgaacttc acgtgagtag taaatattac caccattttt atagagcttc cctcctgaaa
    17581 ttaaggcgag aggatgatag taaaagtaag tgtccccgtt ataatttcct aggctgggag
    17641 ctacaacatt tttagagtaa tttttggcca actcaatgga attttcaccc ccatgatccg
    17701 ctacataatc aatataagaa gaaccaaaat tatatgcttg gacagcagtc catacatcaa
    17761 ccttagcttt tttagcttga cttatatttt gtgacaacag agtgacgcca tgtttaatac
    17821 tggtgtgact atccgtaata ctgttcgtag ttccactgat actttcactt gattgcatca
    17881 catctatagc atctccctta gtttcagtat aaatcataga taatactaag tgcacattag
    17941 cctctgaacc attctctgca agagtattct gcactaaatc atgatattga agcacttttt
    18001 taacattttc atggatgata actagacggt acccaatgaa aataataata attaatgcta
    18061 tcaaacgttt taaaaattta aacatattat ttattttgaa tatcctgtat gtttttgatt
    18121 agtattgagt atgtaccatc agggttttgg ataaattcaa ctgattcggc atcttggtag
    18181 acattgttgg gtacaatcaa ttcaatacca tttgaaagag atagcttctg attctctaac
    18241 ttcttaattt gacgggaatg atcaatatca cttacttgaa cagatccagg tattgcgtct
    18301 ttcaattcat caacaaaagt cagcctggct gttaaattat catcaaataa ttgatctgct
    18361 aatttttctg gagacaattc ttgctcttct tctaaattct tataaatagc cgatttcatt
    18421 tttgattgga aagaaaaatc atctttgttg aaattttcag caattttttg agctgtctgt
    18481 tcaaccattt ttatagattt tttaacagac tgttctggtt gtacttgtaa taaattttca
    18541 gaaaaataat tagcaaatga accattgtgt ttgatacgct tttcaattaa atagtattgc
    18601 tttgatgact tgttaacaac caaagcttca tctggagttt gagcagcact tggaaggtta
    18661 ttctgtgtta ttgtaattgg ctgctcttga ttttcagaaa catgagcaaa atgttccttt
    18721 aatgagattc gaaggaaagc aaaatgttcc attccatcct tgtcaaattg aataaaaact
    18781 aagtcattcg ttttttgatc ttccgatatg acaaactctt ctttccaaag ttgcgcaatt
    18841 tgacaggaag aaacatataa atcatcttgt aaatgactca taaatacatt atcctctcca
    18901 aaatagccac gcttagcttc atcagaaaaa actttactta attttttacg aaagtagtca
    18961 tcaatccttg gagttagggt taacggagta tctgatagca ctaactctgt gtcgttagga
    19021 gaaaactgat gtataataat ctgtttgata taaaaatcca tcataacatt aattctcata
    19081 caaagggaaa gcgtccgtta atgatttaat ttcttgacga acttctgtga ggacatcttg
    19141 attttcatga tttttgagtg ccttaatcat taattcagcg attcttcgag attcttcaac
    19201 tcccattcct cgactagtaa ttgctggagt accaattcga atacctgatg ttttaaatgg
    19261 tgatagtcgc tcaaacggta tagagttctt gttaagggtg atattgactt cttcaagaac
    19321 attttgtgcc tttttgccat tctcaataac ttttgtgacg tctactaaga acaaatgatt
    19381 atcagtacca tcagaaatca aatgaaagtc atcatcttct ttaaaaactt tagccatggc
    19441 ttgagcattt ttaataatat cctcaccgta tattttaaag gaaggatcta gagcttcttt
    19501 caaagctaca gcttttgcag caattacatg ttctaatggt ccgccttgga gtcctggaaa
    19561 gactgcagaa ttaatttttt tagcaattgc ttcatcattg gttaaaatca aaccacctct
    19621 aggtccacgt agagtcttat gtgttgtcgt tgtcgttaca tgagcataag gaataggact
    19681 aggatgatga ccagaagcaa ctaaacctgc aatgtgagcc atgtcaacca ttaagtaagc
    19741 atctactgcg tctgctattt gacgaaactt ttcaaaatca ataatgcgag aataggccga
    19801 cgcccctgca acaattaatt taggttgcgt ttcctgtgct attttcaaaa tgttatcata
    19861 gtctagcatc tccgtcttag gatcaacaga gtaagaaaca aaatgataag tttttcctga
    19921 gaagctaact gaagcgccat gtgttaagtg cccacctgct gctaagtcca tccctaagac
    19981 agtatctcct ggctcgatta aagccatgta agctgctgca ttagcctgac taccagaatg
    20041 tggttgaaca ttagcaaact cagcattaaa taacatctta gctcgttcta tagctaaact
    20101 ttcaactaca tcaacacaat ctgttcctcc ataataacga tgactaggat acccttcggc
    20161 atatttattt gtcaaaacag aaccttgtgc tgccataaca gcttttgaaa caacgttttc
    20221 tgatgcaatt aattcaatat tattttgttg gcgaatttcc tcatcatgaa tagcctgcca
    20281 aagttcttgg tcaaattctt taaaattgtc tttatcaaaa atcatttttt actccttaag
    20341 ctcgttataa cttttaaata atggtttcaa tcattatatt tatcacattt ttactattag
    20401 ttgcaagatg attaaattaa ttttcaaaaa ttaattcagg tatcgtagcc gttagtacct
    20461 cttgcgtgat tgcaccttgc cttaaaatct taactctatc accagacaaa tcaataatag
    20521 tagaatccac acctgtcaat gctttatcat cttcaatacc taaaacttga tgattaaact
    20581 gtttttgaat ttctgagaat acacgaccac tatcacatcc agaaatatta gcagaaggac
    20641 caatcaatgg accatggtta gcaatcatat caagggttac agggtgctta ggcatccgaa
    20701 atcctacgct tgataaccca gaattgatcc aatccggaac ttcttgtgaa gcttccaaaa
    20761 taatggttag cggtcctggt aaaaaagcat cgtacaattg cttcaaatag gtcggttgat
    20821 ttttagaata ttttaaaata gtctcaaaat cacatatatt taagttcata gccttatcac
    20881 gaggtctttt tttaagctca tagacgtggt tcacagcttc ttcactcaaa gctttggcaa
    20941 agattccgta gaccgtttct gttggcataa taactgcgcc atgatcctct aaaatctgtc
    21001 ccaaatcatt cattatctaa taccaccatt ctatctttac caaatatatc tttcaaaact
    21061 ctgcaacgat tgtttggaaa atatcttttc aataaacttc gtaaatcatc accttgtttg
    21121 taacctattt caaaatataa tttacctcta ggttgcaaat attctcttga gttttcaatg
    21181 attttacgat aaatagctaa tccttcttcg tctgcaaaaa gggcactatg aggctctgaa
    21241 gctagcacat tcttccccac ctcgtctttg tcattgtaag aaatataggg aggattagac
    21301 acaataatat caaatttatc tgaaatgtta ctaaagacat ctgattctat aaaagtcact
    21361 tccgcatcac aattattagc attttccttt gccaagtcta atgcagatac agaaatgtca
    21421 ctagctagaa catcccaaga ggggcgttct ttttttagag aaatggcaat tgctccacta
    21481 cctgtaccaa tatctaaaac agaacaatct tctactttat tctctgataa gattagatca
    21541 accaactcct ctgtctcagg cctcggaatt aaaactcgtt catcaacaaa gaaaataaga
    21601 tctctaaagt aagctttacc tgtaatatac tgcggtgatc tatgtttttt aagctgttga
    21661 aaaatatttt ccattaattg ctgatcagta tcactcacac tactattttg atttaaaagg
    21721 taatcagttg ttgaccattg ttttaggtct tttaatacat aaataaaatt ttcgacttct
    21781 tcaccacaag cctctaacaa ctgttcataa tgcttgatta actgcgcata attcattatt
    21841 tatttaaggc ttctaacttt tgagtctgat catacataac aagtgcatca ataacttcat
    21901 ccatttttcc agataaaata gtatctaatt tttgaagtgt caaaccaata cgatgatctg
    21961 ttacacgatt ttgtgggaaa ttataagtgc gaatacgttc tgaacgatca ccagtaccaa
    22021 ctgttgattt acgttcagca tcttgttcat cttgagcaat ttgcgcaaaa tggtcagcaa
    22081 cacgagcacg aatgattttc atcgctttat cacggttttt ttgctgagta cgctcttctt
    22141 gcatttcaac tttgattcct gtaggaatgt gtaccatacg aactgcagta gcaactttat
    22201 tgacgttctg accaccagca ccagacgcgt ggtaaatatc gacgcgaaga tctttttgat
    22261 caatttcgta ttctacttct tcaacttctg gcattaccag aacagttgca gttgaagtat
    22321 gaacacgacc ctgactctct gtcaccggaa cacgttgaac acgatgagct cctgattcat
    22381 atttaagctt agaataaact gactgaccag aaaccatagc aaccacttct ttaataccac
    22441 caacaccatt aacggatgat tccataactt caaaacgcca tccttgagtt tcagcatatt
    22501 tttgatacat tgttaaaaga tcaccagcaa aaagcgctgc ttcatcgcca ccagcagcac
    22561 cacgaatttc taaaataatg tttttatcat cgttaggatc tttaggtaat agaaggattt
    22621 ttaatctttc ttcatactct tctttagctg cttttgattc tttaagttct tctttagcca
    22681 tttcttccaa ttcagcatca ccagaagcat ccttgatcat ttcttcagca tctgaaatat
    22741 tttgaatgac ttgcttatat tctctataag ctgtgacagt ttctcgagta ttagcttctt
    22801 cacgtgataa ttccataaat cgctttgtat ctgaaacaac atctggatca ctaagtaact
    22861 cacctaactc ttcataacga tcttcaactg cctgcaattg atcataaata ttcatttttc
    22921 tttgtatctc ctttaaaacc gattatcttt taaatatctg gattaaaata atgttttcga
    22981 caaactggga tatatgtttc attaccacca atttgtatct gatcaccttc atagacaggt
    23041 tttccgtttt ctgtccttaa aaccatggtt gctttcttac tacagtattg gcaaattgtt
    23101 ttgatttcat caatcttatc ggccaataat agcaaatgtt ttgaaccttc aaaaagattg
    23161 ttttggaaat catttttcaa accaaaagcc atgactggta catctaagtc atctactact
    23221 ctagctaaat cgtagacgtt tttcttactc aaaaattgac attcatcaat taaaacacaa
    23281 taaggctttt gtggtaaatt ctgaatatag gagaaaatgt ccatatcatc tgaaattgga
    23341 actgcttcac gacgcattcc aatacgactt gaaacgactc caaattcatc tcgagtgtcc
    23401 aaggcgcttg tcataataac cacaggtttt ccttgttctt catagttatg agcaactttt
    23461 aaaatttcaa tggttttacc agaattcatt gtcccatatt tataatagag ttgagccaaa
    23521 gcggaaaatc ctactttcta tagtattcta cctattttac cacaaaggct gaagttttcc
    23581 cagccattga aaacagtgtg tactttttca caacattatg ttacaatgat gcccaataac
    23641 ataaaggagt aatattatgc catttgtaaa aattgacttg tttgagggac gctcacaaga
    23701 acaaaaaaat gaattagctc gcgaggttac tgaagtagta tcacgcattg caaaagcacc
    23761 taaagaaaat atccatgttt ttatcaacga tatgcctgaa ggaacctact atcctcaagg
    23821 tgaacttaag aaaaaataag aaatagtaaa agaggttacc caggtaacct cttttgatag
    23881 tctttgcaag catatttctt gacaaattga aaatatttga aaataagtat tgattttcaa
    23941 gttagaaaac ggtatcatta gaatatattg ctttgggggt ggaaaatttg caactttctc
    24001 atagtttaag actcatggga acgactatcg atattcaaat taattctaaa aatgctcaaa
    24061 aacaaatcca agaggtcata gaactcttgg agctctacaa aaatcgcttt agtgcaaatg
    24121 actttaattc agagttaatg gctataaata acaacgctgg tatcaagcct attcaagtac
    24181 atccagacct ctttgaatta atcacaattg ggaaagaaca cagcttagct aggcctagta
    24241 acctaaatat tgctattggt cctttagtgc aaacttggag aattggattt tcagatgcta
    24301 aactacctag ccctagtgaa gtatcagaag ctatgatttt atctgaccca actcatatac
    24361 ttctagattc aaaaaatcaa tctgtttttt taaatcagat aggaatgaaa attgatttgg
    24421 gtgcacttgc aaaaggatat attgctgaca aaattatgac ctatctcaaa aatgagatga
    24481 ttgattctgc tatcattaat ttaggcggaa acgttttagt tcacggagat aatcctaatc
    24541 gttcagaagg gtattgggtt atcggcattc aacatccgaa aaagaaacgg ggacaaaata
    24601 ttggagctgt caaaattaaa aaccaatctg ttgtaacctc aggtacttat gaacgccgtc
    24661 tgattattga cgataaggaa taccatcata tttttgatcg acagactggt tatccgatac
    24721 aaacagagat ggctagcatt tctattgttt ctaaacaatc cgtcgactgt gaaatctgga
    24781 ccacaaggtt gtttggctta tccattaaag aggctcttga tattttaaac gctgtttcct
    24841 acattgaagg cattatcatc actaaagatg accgtatcta tttatctgat ggcttaaaac
    24901 atcattttca actattttat cattaagaaa gaggatttct aatatgaaac ttattggcat
    24961 cgttggaact aactcaaata aatcaacaaa tcgtcaatta ttacaataca tgcaacaaca
    25021 ctttgctgac aaagcagaaa ttgaactcat cgaagttaaa gaccttccgt tatttaataa
    25081 acctgctgat aaaaatgttc cccaagtaat tttagatatt gctgctaaaa ttgaagaagc
    25141 tgatggtgtt attattggta cgccagaata tgatcattct ataccttcgg ccttaatgag
    25201 tgttttagct tggctatcat atggtattta cccacttctc aacaaacccg ttatgattac
    25261 tggtgcttca tatggaacac ttggatcatc acgtgcacaa ttacaattac gtcaaatact
    25321 taacgctcct gaactaaaag caagtgtttt acctgacgaa ttcttgttat cacactctct
    25381 ccaagccttt gataaagatg gcaacctaca cgatatcgaa acaagtcaga agctagatgc
    25441 tatctttgat gattttcgtc tttttgttaa gattactggt aaattatcaa atgctcgtga
    25501 tttacttcaa aaagaagcag aaaattttga ctgggaaagt ttataagagt atagaaggga
    25561 ataactaaga tgaaatttgt aggtattgtt ggctcaaatg cagagcaatc atataatcgt
    25621 atgttgcttg aatttattcg caagaatttt aaaaccaaat ttgaattgga agtccttgaa
    25681 atcgatgata ttccaatgtt taatcaagtc caaaattggg aagaaagttt ccaacttcgt
    25741 cttcttaata ataagatcac acgagctgat ggcgttatta ttgccactcc tgaacataat
    25801 catactatta cggccgctct taaaagtgtg cttgagtggc tctcatttgc tgtgcatcca
    25861 ctcgaaaata aacctgttat gattgtcggt gcttcatatt atgatcaagg cacatcacgt
    25921 gctcaaatac accttcgtaa aatccttgat gctcctggcg ttaatgctta cacacttcca
    25981 ggaaatgaat tcttattagg aaaagctaaa gaagcctttg atgataatgg aaacattatc
    26041 aatcctggga ctgttcaatt tttagaaact tgtcttgata attttatcaa atatgtagga
    26101 gttgtatcat cattgagaaa acctaaagct attgaatctg aagacctcta ctgtcaggga
    26161 caaatcgata caaccattac tagtatcgat ccagatgatc ctgattggtt agacaaggca
    26221 gccgagaaag ttaacgccgt tgaaggggat gcttacgtta aactagacaa tggtttactt
    26281 actgttaacc aacttaatat gtttttaaat gctatgccat ttgagctaac atatgcagat
    26341 gataataatc aatttctata ttacaataat aatcatccag atccagatac aatgttagga
    26401 aaacgtgttc gtgaacaagt aggaaaccgt atggccacag ttcatggtag tttaccaccg
    26461 gcacgaatga aaaatgttga atgggttatc ggtactcttc gtaatggaaa tcaagaatat
    26521 gtacgtacag tcgttcctgg tacacctcca gaagttatca atactcacaa ctatcaagct
    26581 atgtactacc cagacggatc ttatgctggt attaatgaaa ttatctttaa ctttaaaccg
    26641 tggttagatt ggtacctcca aactactgga caacgtttgg tcggtggaac tactgctgct
    26701 cctggagctt cagcagttga cgctacttct agcgcttcaa ctagcacaga ggatgctccc
    26761 gttgatcaag ccacagatgc cacttctggt gcctctgaac attaatctcc tcgcttttga
    26821 ggatctgata ccttaaaaag atatcaagat cctccttttt aatatttaat caagttaata
    26881 aaatcacata aaaggaatat actatgtcac tttttcaaga aaaaattgct tacaattgcg
    26941 ctaaaaagga agcgctttat aaagagagtt taggacgcta cgccttgaga tcaatgctag
    27001 caggggctta tttgacaatg agtactgctg ccggtatcgt cgcagctgat actattggta
    27061 aaatttctcc tgctctatca ggttttgtat ttgctttcat ctttagtttt ggacttattt
    27121 atgttttaat atttaatggt gaattggcga catctaatat gctttatctc actgcaggag
    27181 cctataataa aaatatctct tggaaaaaag ccataacaat tttaatttat tgtacttttt
    27241 tcaacctcgt tggtgcttgt atattagctt ggttgtttaa tcaatcgtac tcattccagc
    27301 atttaacaaa tgatagcttt ttagggcatg tcgttgctaa aaagttagga aaacctagtt
    27361 caggagcttt cctagaaggt attattgcta atatgtttgt gaatttagct atccttgctt
    27421 acatgttatt aaaagaagaa tcggcaaaaa tgacggttat tttatctgct atttttatgt
    27481 ttgtttttct atcaaacgaa cacttaattg ctaattttgc atcctttatg ttagctgctt
    27541 ttagccatat cgaacatatt aaaggtttta ctcttctaaa cgttatccgc caatggacac
    27601 ttgtattttt tggtaactgg ataggagggg gcgttttcat cggcctagcc tatgcttggt
    27661 taaataa
//
LOCUS       DATRNX010000012        27361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRNX010000012 DATRNX010000000
VERSION     DATRNX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(125..1321)
                     /locus_tag="VBC81_000431"
     CDS             complement(125..1321)
                     /locus_tag="VBC81_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6911493.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(1456..2325)
                     /locus_tag="VBC81_000432"
     CDS             complement(1456..2325)
                     /locus_tag="VBC81_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6911494.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            complement(2534..3544)
                     /gene="gap"
                     /locus_tag="VBC81_000433"
     CDS             complement(2534..3544)
                     /gene="gap"
                     /locus_tag="VBC81_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6911495.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            complement(3748..5826)
                     /gene="fusA"
                     /locus_tag="VBC81_000434"
     CDS             complement(3748..5826)
                     /gene="fusA"
                     /locus_tag="VBC81_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6911496.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            complement(5981..6451)
                     /gene="rpsG"
                     /locus_tag="VBC81_000435"
     CDS             complement(5981..6451)
                     /gene="rpsG"
                     /locus_tag="VBC81_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6911497.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(6473..6886)
                     /gene="rpsL"
                     /locus_tag="VBC81_000436"
     CDS             complement(6473..6886)
                     /gene="rpsL"
                     /locus_tag="VBC81_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6911498.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(7105..7917)
                     /gene="purR"
                     /locus_tag="VBC81_000437"
     CDS             complement(7105..7917)
                     /gene="purR"
                     /locus_tag="VBC81_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6911499.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            complement(8014..8955)
                     /locus_tag="VBC81_000438"
     CDS             complement(8014..8955)
                     /locus_tag="VBC81_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6911500.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            complement(8945..10219)
                     /locus_tag="VBC81_000439"
     CDS             complement(8945..10219)
                     /locus_tag="VBC81_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6911501.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            complement(10221..10853)
                     /locus_tag="VBC81_000440"
     CDS             complement(10221..10853)
                     /locus_tag="VBC81_000440"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6911502.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            complement(10846..11508)
                     /locus_tag="VBC81_000441"
     CDS             complement(10846..11508)
                     /locus_tag="VBC81_000441"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6911503.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            complement(11515..12387)
                     /gene="rsgA"
                     /locus_tag="VBC81_000442"
     CDS             complement(11515..12387)
                     /gene="rsgA"
                     /locus_tag="VBC81_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6911504.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(12543..13394)
                     /locus_tag="VBC81_000443"
     CDS             complement(12543..13394)
                     /locus_tag="VBC81_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6911505.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            complement(13398..14270)
                     /gene="rsmA"
                     /locus_tag="VBC81_000444"
     CDS             complement(13398..14270)
                     /gene="rsmA"
                     /locus_tag="VBC81_000444"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6911506.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            complement(14350..14841)
                     /locus_tag="VBC81_000445"
     CDS             complement(14350..14841)
                     /locus_tag="VBC81_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911507.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            complement(14860..15420)
                     /gene="rnmV"
                     /locus_tag="VBC81_000446"
     CDS             complement(14860..15420)
                     /gene="rnmV"
                     /locus_tag="VBC81_000446"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6911508.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            complement(15407..16189)
                     /locus_tag="VBC81_000447"
     CDS             complement(15407..16189)
                     /locus_tag="VBC81_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6911509.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            complement(16371..16652)
                     /locus_tag="VBC81_000448"
     CDS             complement(16371..16652)
                     /locus_tag="VBC81_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911510.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            complement(16662..17054)
                     /locus_tag="VBC81_000449"
     CDS             complement(16662..17054)
                     /locus_tag="VBC81_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911511.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            complement(17045..18337)
                     /locus_tag="VBC81_000450"
     CDS             complement(17045..18337)
                     /locus_tag="VBC81_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6911512.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            complement(18337..18729)
                     /locus_tag="VBC81_000451"
     CDS             complement(18337..18729)
                     /locus_tag="VBC81_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6911513.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            complement(18904..20166)
                     /gene="dltD"
                     /locus_tag="VBC81_000452"
     CDS             complement(18904..20166)
                     /gene="dltD"
                     /locus_tag="VBC81_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6911514.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            complement(20159..20398)
                     /gene="dltC"
                     /locus_tag="VBC81_000453"
     CDS             complement(20159..20398)
                     /gene="dltC"
                     /locus_tag="VBC81_000453"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6911515.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            complement(20413..21678)
                     /gene="dltB"
                     /locus_tag="VBC81_000454"
     CDS             complement(20413..21678)
                     /gene="dltB"
                     /locus_tag="VBC81_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6911516.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            complement(21675..23210)
                     /gene="dltA"
                     /locus_tag="VBC81_000455"
     CDS             complement(21675..23210)
                     /gene="dltA"
                     /locus_tag="VBC81_000455"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6911517.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            complement(23223..23345)
                     /locus_tag="VBC81_000456"
     CDS             complement(23223..23345)
                     /locus_tag="VBC81_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6911518.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            complement(23356..24543)
                     /locus_tag="VBC81_000457"
     CDS             complement(23356..24543)
                     /locus_tag="VBC81_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6911519.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            complement(24543..25217)
                     /locus_tag="VBC81_000458"
     CDS             complement(24543..25217)
                     /locus_tag="VBC81_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911520.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            complement(25573..25707)
                     /gene="rpmH"
                     /locus_tag="VBC81_000459"
     CDS             complement(25573..25707)
                     /gene="rpmH"
                     /locus_tag="VBC81_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6911521.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            complement(25891..27246)
                     /locus_tag="VBC81_000460"
     CDS             complement(25891..27246)
                     /locus_tag="VBC81_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6911522.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
BASE COUNT         8450 a         5485 c         4389 g         9037 t
ORIGIN      
        1 aaataccact atatctaaaa actagtaaat gataaatgaa gtcaaggaaa gttcaataac
       61 tctacattat caactaaaaa aagacatgca tcagcatgtc ctttaatata aattataatg
      121 actattattt ttcagtcaat gctgccaaac ctggtaatac tttaccttcg agcaattcca
      181 tgcttgctcc accaccagta gagatccatg agaatttgtc agcacgacca aggttgatag
      241 cagctgctgc tgaatcacca ccaccgatga ttgatttaac gcctggttgt ttaacgatag
      301 cgtccattac accgattgta ccagcttgga agtcagggtt ttcaaagaca cccataggtc
      361 cgttccatac aactgtttta gcaccagtaa gtgcttcatc aaatttagcg attgatttag
      421 gaccgatgtc aagaccaagg aacccttctg aaactgcttc accttcagtg tcgcgaactt
      481 cagtataacc agcaaatgcg tttgcttctt ttgagtcaac tggcaagatc aatttaccgt
      541 ttgatttttc aaggaggtct ttagcaacat ccaatttgtc ttcttctaca agtgagttac
      601 cgatttcgat accttgagct ttgtagaatg tgtaagtcat accaccaccg ataagaactt
      661 tatcagcttt ttcaagaagg ttttcgataa caccaatctt atcagaaact tttgagccac
      721 caagaatagc tacgaatggg cgttctggag tttcaactgc ttcttggatg taagcaattt
      781 cgttttcaag aaggaaacca gctacagctt tttcaacgtt tgctgaaata cctacgtttg
      841 atgcatgagc acggtgtgct gtaccaaatg catcgttaac gaagattcca tctccaagtg
      901 aagcccagta tttaccaagt tcttcgtcat tcttagattc tttcttaccg tcaacatctt
      961 caaaacgagt gttttcaacc aaaagaactt gtccatcttc caaagcattg attgcttctt
     1021 ctaattttgc accacgagta acacctggga atacaacatc ttgaccaagt ttagcagcta
     1081 aatcagcagc taccggtgca agtgattttc cttctttgtc agcttcttct ttaacacgtc
     1141 caaggtgaga gaagaggata gcacgaccac cttgttcgat gatatacttg attgttggaa
     1201 gagccgcagt gatacggttg tcgttagtga taacgccgtc tttcaaaggc acattaaagt
     1261 caacacgaac gaggactttt ttacctttca aatcaacgtc tttaacagtc aatttagcca
     1321 ttagtaaaac tccttatata atttgatatt atttattata acatattttt gtgaacagtt
     1381 gtaattattg attacttttt cacaaataga tttcatagat tttcctagtt gtttttaaga
     1441 gaattcgtat acttattaat catacgaatg caacattttt tgtcgttctt tcaattgttt
     1501 ttgaacatcc agatgttttc cttgataaat agcactttcc caagaaccgt acataggatt
     1561 tgggaaaaca ataaatttac taccaaactc actttgaagt ttagttagta gttgttctct
     1621 atctgtacta gatgatttag aaaaatcagc aaaatcaact aaattatctc caaaaagcat
     1681 aattaaattg gtatcttttt gaactgcctg acggcgactc tctttagatt tcattccttt
     1741 tttaaggaaa agcaagtggt ctttcccttg aacaggtata ccttccttct ctaaattttc
     1801 tttagtcgca tcaacttgag catctgtacg atctgagaca taataaattt ttattccctt
     1861 ttcattagca tacttcaaaa attctttggc acccgcaaca gccttagctg atttcttttg
     1921 cacccattta tcccaactct ctggcgtgaa actagagcca tctttaatat tctttgcttg
     1981 atatgggcta ttatctaaaa ctgtttcatc taaatctaag ataattgaat atggtttttc
     2041 actgggcttt tgtaaccaat catctaactt cattttagca acattataac cttgtaaata
     2101 taaagccttg gcttctgcag aattttgata ccaaaggaca gacatagtgt tttccttagc
     2161 caacagttta ttagaactta gagaaatatt tttaaatttc gtcttttgct ccaccttatc
     2221 tggagacata tcagcttttt tagtactgtg gtttgactga catgctgtca tcataaatac
     2281 agcaacagat aacgataaaa ctgaaaaaac ttttgtagtc ttcataaaac ttctcctcta
     2341 tattttttca attaaatcat accactgctt actttcaaaa tcaaacctta ttttaactta
     2401 tagtcaatat aattcatttt tcatttattt tctgataatt ttctataata aaatgaaaaa
     2461 acattcgtat tattttttat ttataaaaat aaaagaggga taaatccctc ttttacatca
     2521 tattaattaa aaattatttt gcaatttttg caaagtattc aagtgtacga acaagttgtg
     2581 aagtgtatga catttcgtta tcgtaccatg aaacaacttt aaccaattgg ttaccgtcaa
     2641 cagtttgaac tttagtttga gtagcatcaa acaatgaacc gtatgaaata ccaacgatat
     2701 cagatgatac gattggatct tcagtataac cgtatgaatc gttagctgct gctttcatag
     2761 ctgcatttac ttcttcgaca gttacgtctt tttcaagagt tgcaaccaat tcagttactg
     2821 atccagttgg aacaggaaca cgttgtgcag caccatcaag tttaccgttc aattctggga
     2881 taacaagtcc gatagctttt gcagcaccag ttgagttagg aacgatgttt gcagcacctg
     2941 cacgagcacg acgaaggtca ccaccacggt gtggtccgtc aaggatcatt tggtcaccag
     3001 tgtatgcgtg gatagtagtc atcaaacctt gtttaacacc aaagttgtct tgtaaagctt
     3061 tagccattgg agcaagacag tttgtagtac atgaagcacc tgagataact gtttcagttc
     3121 catcaaggat atcgtggtta gtgttgaaaa caactgtttt aacgtcgttt ccaccaggag
     3181 ctgtgataac aactttttta gcaccatttt catggatgtg ttgttcagct ttttcttttg
     3241 atgcaaagaa accagttgct tcaagaacga tttctacgcc atcagtagcc cagtcaatgt
     3301 ttgctggttc gcgttcagca gaaactttaa caaattgacc gttaacttcg aatccacctt
     3361 ctttaacttc aacagtaccg tcgaaacgac cttgagttgt gtcatatttc aacaagtgtg
     3421 caagcatgtt tggatctgta aggtcgttga tacgagtaac ttcaacacct tctacgtttt
     3481 ggatgcgacg gaatgcaaga cgaccgatac gaccgaaacc gttaatacca actttaacta
     3541 ccattagtga tttcctcctt atgaaaatct caaatatttt attttaggga tccccctaac
     3601 caatgtgaaa agattaactt gtacatatgt gcaaaaatct ttcaacacaa ttattatatc
     3661 ttttttcgag aaaaattgca atgaatttac cactttcaaa aagaaaaaaa gatactcttt
     3721 cgagtacctt tcataagttt tcagaaatta ttcaccagag tttttcttga tgatttcttc
     3781 ttgaactgac ttaggaacat cttcgtagtg gtcaaatacc atcatgaatg tgccacgtcc
     3841 ttgtgttgca gaacgaagaa ctgttgcata accaaacatt tcagcaagtg gaacaaaggc
     3901 acgaacaact tgtgtattac cacgtgcttc cataccatca acacgaccgc gacgtgcagt
     3961 cacgtgaccc ataacgtcac caagattgtc ttctggagct gtaattgtaa caagcatcat
     4021 tggctcaagg atagctggtt gcgctgattt agcagcttct ttaagtgcaa gtgatgcagc
     4081 aatcttgaag gctgtctcag atgagtcaac atcatggtat gaaccatcgt aaagtttagc
     4141 ttttacgtca accattggat atccagcaag aacaccgttt gccatagatt ctacaagtcc
     4201 tttttcaacg gctggaataa attcacgagg aaccacacca ccgacgatag cattttcgaa
     4261 ttcgaatcct ttaccttctt cgtttggtgt aaattcaatc caaacatcac caaattgacc
     4321 tttaccacca gattggcgtt tgaagaatcc acgtgcttgt gtagaagcgc ggaatgtttc
     4381 gcggtatgat acttgaggtg caccaacgtt tgcttcaact ttgaattcac gtttcatacg
     4441 gtcaacaagg acatcaaggt gaagttcacc cattcctgag ataactgttt caccagtttc
     4501 aacgttagtt tcaacgcgga acgttggatc ttcttcagca agtttttgaa gggcgatacc
     4561 cattttatct tggtcagctt tagattttgg ctcaaccatc aattggataa ctggttctgg
     4621 aacttcaatt gattcaagaa taacttttga tttttcatca gtcaatgaat cacctgtagt
     4681 tgtatctttc aaaccaacag ccgctgcgat atccccagca taaacagttt caatttcttg
     4741 acgactattt gcgtgcattt gtaagatacg tccgatacgt tcacgtttac ctttagatgt
     4801 attcaacacg tatgaacctg aattaagaac acctgagtac acacggaaga atgttaagcg
     4861 acccacgaaa ggatctgtca tgatcttgaa ggcaagcgct gcaaatggct cttcatctga
     4921 agcaggacgt gtttcttctt cgtcagtatc agggttaata cctttgattg caggaatatc
     4981 aagtgggctt ggaaggtaat caataacagc atcaagcatc aattgaacac ctttattctt
     5041 gaaggctgaa ccacacaata cagggtagaa ttcaacattg atagttgctt tacgaatagc
     5101 agccatcaac tcttcgttag tgatttcttc accttcaagg tatttcatca tcaaatcttc
     5161 atcagtatca gcaactgctt ctactaattt ttcacgatat tcatttgctt gatcaacata
     5221 ctctgctgga atatcttctt caaggatatc tgtaccaagg tcattagtat agatttcagc
     5281 tttcattttg attaagtcaa taataccacg gaaatcgtct tctgaaccaa ttggcaattg
     5341 aattgggtgt gcattcgctt gaagacggtc atgaagtgat tgtactgaat agaggaagtc
     5401 agcaccgatt ttatccattt tattagcaaa aacgatacgt ggaactccgt attctgttgc
     5461 ttgacgccaa actgtttcag tttgaggttc tacacctgat tgagcatcaa gaactgttac
     5521 cgcaccatca agtacgcgaa gtgaacgttg aacttcaatt gtgaagtcca cgtgtcctgg
     5581 tgtatcgatg atgttaacac ggtgaccatc ccattgtgct gttgttgcag ctgatgtaat
     5641 tgtgatacca cgttcttgtt cttgttccat ccagtccatt tgtgaagcac cttcgtgtgt
     5701 ttcaccgatt ttgtggattt taccagtata gtaaagaata cgctcagttg ttgttgtttt
     5761 accagcatcg acgtgagcca tgataccgat attacgagtt ttttcaagtg aaaattcgcg
     5821 agccatttgg tttctcctat attttaaatt atttacttct ttattataac actatttata
     5881 aaaaacggat aggcaggacc tacccgttct aattgctaac attgctatct accaaacaat
     5941 ttgtttagta gctatgataa gtctcaaaaa gagagtaatc ttaccaacgg aagtgtgcaa
     6001 aggcacggtt agcttcagcc attttgtgag tatcttcacg tttcttaact gatgctcctg
     6061 tgttgtttgc agcatccatg atttcttttg caagacgatc tttcatagtg tgttcaccac
     6121 gagcacgtga tgcgttaacc aaccaacgaa gtcctaaagt tgtacgacgt tctggacgaa
     6181 cttcgacagg aacttggtag tttgatccac caacacggcg tgcacgtacc tcaagtacag
     6241 gcatgatgtt ttccatagct gtttcgaaca cttcaagagc atctgttcca gtagcttctt
     6301 taattgcttc aaaagcatca tatacgatag ttgcagcagt accacgttta ccatcaagca
     6361 ttacacggtt gataagacgt gtaacgattt ttgagttata caatggatct ggcaatactt
     6421 cgcgtttagg cgcttgattt ttacgactca ttttctctta tcccccttct aattaccctt
     6481 taggacgttt agcaccgtat ttagaacggc cttgtttacg atcagctaca cctgcagtat
     6541 caagtgcacc acgaacgata tggtaacgta cccctggaag gtcttttaca cgtccaccac
     6601 ggataagcac aacactgtgt tcttgcaagt tgtgtccgat acctggaatg taagcagtaa
     6661 cttcaataag gttgctcaaa cgtacacgag caaatttacg aagggcagag ttaggttttt
     6721 taggtgtcat agttccaaca cgagttgcaa caccacgttt ttgtggtgca gataatttag
     6781 tgtgaacttt tctgtgactg ttgtaaccaa tattcaaagc aggtgaatct gatttttcaa
     6841 ctttagattt acgtggttta cgtaccaact ggttaattgt aggcatctac atttctcctg
     6901 taagtttttt atttttggtt gatatagcac ttggtgacag cctatatctg cgtgtacttt
     6961 tgcaacaatt gtcagcacgt ctctgtacac ttttgagaga ccaaaagtaa aagtaccgtt
     7021 tatcatagta tcattttaca cataatgtgt caagctttta ttacttttac aagcaaaaag
     7081 aatcaaggat gtcaccttga ctctttaatc aaatatattc cccgcttcta cagagactct
     7141 attatttttt acatcaattt cagatactct cagaagagat ttataggcca tcttctcacg
     7201 ttgttcctga gcattttctg caaatacagc gacacctact aatgtgctat cgaattctga
     7261 gagtaaacta atcatccccg aaacagtacc tccacctttc aagaaatcat caactattaa
     7321 aactcgactg ttaggtttta agctacgctt agaaagaaac attttttcaa tacggccact
     7381 tgaaccacta gcgtagttta ctgacacagt agaaccttcc gtaatcttca aatcacgtct
     7441 cacaataaca aaaggaacat ctaaaacatt tgctactgcg tttgctaaag gaacaccttt
     7501 tgttgcaacc gtcatgacag catcaatttt ttgacctctg taagcgttcg ctataatgcg
     7561 accaatactt tgtaacattt ttggtgtact gagtagatct gaaagataaa tgtaacctcc
     7621 cggaagaata cgatcgtttt cagataaacg ctgtcttaat tcttcaacga tttctttcgc
     7681 ttctgcttca gcaatagttg gtgtaaagat aacacctcca cttgcaccag tcacagtttt
     7741 aatatctccg atttgagctt gttcaaaagc ttttttgata atagcaatat cttctgaaat
     7801 cgaagatttc gcggcaccgt atttactagc aaatgtattt aaactagtta atgtataggg
     7861 attatttatc aggtaatttg aaataacaac catgcgttcg ctacgtctta atttcatatt
     7921 ttctccttat tcatagcatt ctttacatat agtataacat aacaaatgct aaaaacgaac
     7981 attttatttt aggtcttaat tattctacct aaattattta atgttcggtt tataaaaaga
     8041 acggttatcc atggcaaaaa tgcgatttgt catttcacct tcgttgacac ggtttaaagc
     8101 tgttgtcatc atcatcatat ttgcatcaat gttatcgatc atatggataa tttctgcctc
     8161 catgattcta ggacgaaccg ggcttccata ttctaattgt ccatgatgac tcagaataac
     8221 atggcgtaac actgtaacct cttcttttgt atcatcaata ttaagctctg ctagtatttt
     8281 tgttaattcc tcatcaataa gtgaaatatg accgataaga ttacctcgaa tagtatattc
     8341 tgtattatca ggacccgata actcaatgac cttggctaaa tcatgtagca taataccagc
     8401 aaacatcaaa cttttattaa gttctggata gatatctccg atactatctg ccaaacgaac
     8461 cattgttgcc gtgtgatatg ccaatcctga ttcaaaagcg tggtggtttg ttttggcggc
     8521 tggataggta aaaaattcct tattgtattt acgataaagt gctcttacca cacgctgcca
     8581 ggttgcattt tcaattttaa agagcatttg ttccatgtac tctctcacgt tgtcaacatt
     8641 gattggaggt ttttctttaa aatctctagg atcattaggt tcaccatctt tgatgtttct
     8701 taatgtaatt tggttgactt ggggagttcc attataaact tcacgacgcc ctttcatatg
     8761 aacaatttta ccggctacaa actcttctac attataggtc tgtgcatccc acatatttcc
     8821 tgaaatttcg ccagtatcat cttgaaaggt aaaggcaata aagtctttac ctgcacgcgt
     8881 tttacgaact tctgctttct ttataaggta aaacccctca aaaagctcgt cttttttcat
     8941 ttggttaatt ttcattaatt tcctcttctt ctgttatagg aaggtttaac aagctagtgg
     9001 ttgcttgatc ttgatgttct tcaactgtat ttaagactct gataatagcg tttgtccgcg
     9061 ttgtgagtaa actatcaata cttttacttg ctgtatttaa ttgtttttga gcttttgata
     9121 acatgccacc aaattttcca aattcaacct tgacattccc taaaatttta ctaatatcat
     9181 tagcattctt ttgaatattt aaggttttaa agccaacaga taatgaattt agtaaagcag
     9241 ataaggttga cggtccagct actacaatat tttcgtcacg tcttagacta tcaaagaatg
     9301 ttgcatttct taccacttca gaatagagcc cttcagttgg taagaacatg ataccaaaat
     9361 ttgtcgtttc aggaggattt aagtacttat tgtttatatc ttttgcaaat ttacgaatac
     9421 ttgccagtaa agatttacga tagagttcta tttgaacctt gtcacccaat tcataagcat
     9481 cttccaatcg gtaataatct tctagaggaa acttagagtc aataggcaaa tagatataat
     9541 ctccctgacc atttccaggt aatttaatag catattcaac acgctcagaa gagccagaca
     9601 ccgtaggaaa ttctctctca tattgactaa ctgtcataat atcttctata atttggccga
     9661 gttgtaactc ccctaatatt ccccttgtct tagtatttga caagactttg ttcagtgtac
     9721 caacatcttg ggcaacagtt ttcatctccc caagaccttg attgacgctc tctagttgac
     9781 gcgatacagt ttcaaaagaa gtttgcaaac gcgtttgtag cgttttttct agcttttcct
     9841 caacagtttg gcgcatttga tccaagcgtt tttcattgga atcttgcatt ttctgaactg
     9901 actgagataa attggagtta atcaattcca agcgtttgtc tgatcgatct cgactctccg
     9961 ttaaatgctg atttaattct gtacgcatat cggtaagcaa ctgataaatt tcctgttgca
    10021 tacggtttag ctgattagtc aacgttagaa tttgatcttt ttgcgccact tctatctgat
    10081 atgtcagttg gtctgataga ttatcagcat tatcttcaag ttgtttttca acggactttg
    10141 ttaattccgt catttttggg taagaaatta tcgtcactgt cagactagct agtacaatga
    10201 tcaaaatgat gacaagcata ttaacctcta tccttactat aaataatgat taagtaacca
    10261 tctttgagct ctacttcaat tggcgaagtc ataaattcat tactggaata catttttttc
    10321 ttaaaataat tacttttgtt caattcatat ttagccccag aaatctgcaa agtagactca
    10381 ctctcaggca tgaacgagac ataggacatc ccttcaatcc ttgagacttg atttttcccg
    10441 gatggtagat aagtcactat gttttgttca tctttgagtc tgatttgtga cataaatggt
    10501 tctagatctc tgtcacttgg taaaaagata ttagacatca tatggtcaat cctgccacca
    10561 aatgctccaa atacggtcac ctgagcttct ggaaattctt tgaaaatagt ttttaaagct
    10621 aattctgtat ctgtatcatt tttttctgca ctagcagaaa cgatattgct acaatagtgt
    10681 ttgatataaa gcagttcatc ttctgttata gaatcaaaat cacccactgc catgtccaaa
    10741 gaaagcccat tttttaataa aaataggctt ccacggtcaa ttccgacaaa atagtcaaaa
    10801 tcatattcaa aataagttaa gtcaccgcca gcaaataaag caattttagt catcgagcgc
    10861 cacccttaag gtttctactt gtgcaggtaa atcacttgct ttaaagagat aagaacctgc
    10921 aacaaagaca tttgcaccag cctctgctgc tgccttaatt gtagtattat ctataccgcc
    10981 atcaacttca atatcataat gatgaccgta ctctttgcgc catgctgcaa ctgtctttac
    11041 ttttgacatc atttctggaa taaaggcttg tccaccaaag ccaggattaa cagtcataat
    11101 taaaatctgg tcaaccaaat ctaagatagg aataagagat tctacgggtg ttcctgggtt
    11161 aataaccaca cctgctttca ttcctgcctc ttttatcttt tgaagcgcac catgtatatg
    11221 tttcgttgct tcaacatgaa tggtcatgat atcagcacca gcttgagcaa aagcctcaat
    11281 ataacgttct ggatcaacaa ccatcagatg acaatcaaaa acaagtttac tgtgtttacg
    11341 catgctggat acaacatccg caccaaaact aatattaggg acaaattgac catccatgat
    11401 atcaatatga acgtattctg ctgttgtctc ttcaatacgt ttcaattcgt tagcaaaatt
    11461 agcatagtct gctgctaata tcgatggggc aattttattg gttgacatag aaacctactt
    11521 tcttttaata actttcttat atgtttcacg acggttctca atttcactta aaaattggag
    11581 gtagttatca tagcggactt gccataactg accttgagtt aaggcttctt ttacagcaca
    11641 tttaggctca tgtgtatgcg tacacgagcg gaatttacaa aaatgactaa gtctacgaag
    11701 ctctggaaag gattcgttta agtcctctgc attatccact tcatagtcca aagaagaaaa
    11761 accaggagtg tctgcaattt ttcctttatg aacgttatag aaactaactg ctcttgtggt
    11821 gtgtcggcca cggcctaaac tacccgaaat ttcaccagtt tcaagtttca actcaggagc
    11881 aattttgttg agaagcgttg attttccgac acctgtctgg cccataaaaa cagtcacttt
    11941 atttgctaac agaggcaaga gttcctcttg agaataacag aaaacgtagc ctatattctg
    12001 ataaagttcg cgaatatcat caataacaac caaatcgtct aacaaatcca acttggaaat
    12061 ataaatgata ggctgaatca tcttatattc tagaagtact aagaaccggt ctaataagtt
    12121 agcgttaaaa tcaggttctt tagcagacat gataacgaca gcttgatcaa tattaacaat
    12181 aggtggtcgt acgaggctat ttttacgctc ttcaattgat agaatatatc cttctgattg
    12241 atcttgcgaa gaaaattcaa cccaatcccc aacgtaggga atttgacctt tttttctaaa
    12301 atttccacgc gcacgtgtct gataaacaac accgtcagat tccacataat aaaaacctgc
    12361 tagcgacttg acaattctac cttgcaattg ttctccttaa ttgtttagaa taccactatt
    12421 atatcataaa atgagaaata agctctactc tcagagataa gctaaataga aactatttgc
    12481 aggtgttcga tttctctcaa ataacctcaa ttttatgaat tcccaacata aaagcagtga
    12541 tcttatttag atacactgcg cttttttcca attactatta aggcacctac tgtaatttct
    12601 gatatactcg cccaatcaat agtcgtttta tctacgctga ataacaacgg tgtcatatca
    12661 ataaaagctt gacgttcttg ttgagaacag ttatagcttt gaacaacttt ctgctcagaa
    12721 ataatttcaa aattttctct aaagtgatct agaatttttt gattagagta atctttctgt
    12781 ttcaagtatt gactagcttt ttctcgcaat tcttgaacat gttcagctac aggaacaacc
    12841 tttacaagga taccatcatc actcagcacc cgcctaaatt ctttataatt tgcaggagaa
    12901 aaaatgtcta aaatgatatc aattgatgag tcttgaatag gaaggttagc taaatcagaa
    12961 acaaaccatt tcacaagcct agactgatca cttttagcag ctaattgaat cgaatcttta
    13021 gaaatatcaa aagccaagaa agtcttctca tgcttgttaa ctaattgacg agaataaaag
    13081 ccctctccac aaccaatatc taaaaccgaa tgaaactgag aattattttc caaaaccttt
    13141 gaaatagctt ctaaaatatg attataatac ccgttttcta agacaagctg ccgattttca
    13201 aatgattttt tatcataatg ttcatctacc ttctttcctc caagaagatt aacatatcca
    13261 aatttggata aatcaaaaca atgattatca gagcatttga ggctgttact ttcttttatc
    13321 aagggatttt gacatttagg acaagcgaaa taacttgcac tatcttcaaa acgccacttc
    13381 ttgtcaacca acatagttta tatcccaacc tctttcaagg catctgctaa acttgcaaaa
    13441 tcagggatag ataaggcttc tccgcgaata gatggcttaa tttttgctat ttctaatgct
    13501 ttttccaatt ttgctttagt atcttctgat tttccaaaat gactcgttag attattccaa
    13561 agtgttttac gacgatgtac gaaggctact tttgacactc tgaaaaagaa atcctcatct
    13621 tgaacactca ctactggttg atctctacag accattttca aaatagctga atctacattt
    13681 ggagcaggaa caaaaacagt tctcggaacg ataaatgata ctttggcagt catataatac
    13741 tgcactgcga ttgacaaaga accgtatgct ttagtatttg gcatagctga aatacgatct
    13801 gccacttctt tttgtatcat gaccacaaac tccgcaaaag gaatcttgct ctcaatcaag
    13861 tgcataagga ttggtgttgt gatgtagtaa ggtaggtttg cgaccacctt gataggcaga
    13921 tcaggattct tgaaagcctg aatctgggtc tgcaaatcag ccttgaggat atcctgattg
    13981 accacttgga cattgtcaaa acgcgcaagc gtatcagcaa ggataggaat gagacgatca
    14041 tcaatctcaa aagccataac ctcagctgca ttctcagcca aaaactctgt caaagccccg
    14101 ataccaggac caatctcaat cacgttgaca cccttgtcaa tctctgccgt gtcaacaatc
    14161 ttctgtaaaa tattagtatc ggtcaaaaag ttctgaccga atgatttctt aaatgtaaac
    14221 ccatgacgct ccaaaatagc gcgggtaacg gttttatctg cgattctcat aattcctcta
    14281 tttcttaata ttgatcattt ttgaaataac atttgaggct atttgtgata ttacaactgg
    14341 attataccac tacttccttt tgcctttact attctgagtt ataaacttat ttttaattgc
    14401 agttgcacaa gcttcggtta gtggttctcc tattgcttgc ttataattaa atccaatcaa
    14461 ctgcattgtt tttaaagtat ctttattcaa taatgtaacc aattgtttca ttgttcgttc
    14521 ctgttcctca tttagaccag aaaaattata taacaatgag attccttgct cgagagtatt
    14581 ggatagatat tcaatatcct catcatctat attaattttt tcatactctt ctttatatga
    14641 ctgtgcaatg cgaattgctt gctcccgttc agctaataat tcgttaatta aatcatcata
    14701 agtagctctc aatgttgcag catcttttac atcctttatt gattgaattt tactggttac
    14761 catagttgca gtccctttga ctgcaagcgt tgctaaatct accccagctc tagtcaatgc
    14821 ttccgttaca ttatactcca ctatttttct ccttttctat taatacccct ccatacactc
    14881 ttcaacttct gccttggtca ctccgaagag tctgaggcgt ttgaggagtt gtttaccatt
    14941 tgagtagccg atacggagtt gattcccaag atactcacgg cgttttcggc tatcgctagc
    15001 agtaatgaat ccccaacgaa tgaggtctgc ttgggtgata tcgaaatgat cttcatcatc
    15061 gaaatgctga gtcacttttg agagtgcctt ctgcaaatct tcaaagctgg catgctcgac
    15121 acccagagat cgtcccttgg tctttgagcc tggctttgcc tcgtctcgat tgaggaaggc
    15181 atggcggact gtcggaatgg cattcatgat gattttacgg atacgctcgc cattataatc
    15241 tgggtctgta aagacgataa ctccccgcaa attgtgcaaa cgttcgatac gctctaaatc
    15301 atcttcatca attgctgaac ctctagtttc ataggtatca acattgtaaa agcgtcgcag
    15361 attagcagta tcatctttcc cttcgacaac gatcacttct tgaatatcaa tttttttcat
    15421 caagtctaaa tatccttact gcattttggt atgtagcctc cgctacctct tccacagtta
    15481 tgcctcgtag ctctgctatt ttttcaacga catatctcgt ataagcggtt ttgttttctc
    15541 gacctctttt aggtacagga gccaaataag gagcatctgt ttccactaat atcttatcta
    15601 atggcaactc tcgtgcagct tcttgtacat ctaatgcctt cttaaaggtc acaacaccag
    15661 aaaaggaaat catcatccct aaatcaataa atttctgagc catttccaaa gaaccagaaa
    15721 aagagtgcat tataccacca aaaggcccaa ctccagattc cttaatgact tcataagtgt
    15781 cttctaacgc atcacgtgta tgaacgacaa aaggaaggtt atactctttt gaaagttcaa
    15841 tttgacgctt aaacacctcg atctgaatat ctttgggatc ctccatccaa taataatcca
    15901 gaccaatttc accaagtgca ataaccttgg gattttctaa atgactgata atcattgact
    15961 caatattatc atcataagaa ccagcttcag ttggatgcca tcctattgtg ctatatacct
    16021 gtgcatattg ggaagataat tccaaagatt ttgaaatggt atcttggtca aagccgacaa
    16081 cattcatttt tgtaacaccg agttcgctag ctaaattaat ttcctcgtct atctttcctt
    16141 caaaattttc tacgttaagg tgggtatggg tatcaaatat ttttatcata tcatcgttct
    16201 ttctttgctt atcgtagcta caagcattaa tctttattat tataacataa gactgaagca
    16261 ttctgatcag aaagagtaaa tcaaaaaagt caaaacattt ttgctctgac ttttgaatac
    16321 ttgctgtaaa aatcctctac aagtgtctag ctaatggtag agaaactaga ctataaatga
    16381 ttatattttt gctgattgct ttcaatagcc tcctcaacct ttgaacgaaa ttctcctttt
    16441 attgcctcgt ccaattgatt agcaacttta tgaatagctg tctttgtgtt gatggctact
    16501 tcgtctattt ctttatgatg cgacgtaata atatcgtgaa acttagatct cttgcgtggc
    16561 gcgtacacgc tatgtatcaa ttctttatcc aattgttttt ttaaagtcct tactgactcc
    16621 ttaccagtca ggacatccca cattaaactc aataatttct cctacttcat taattctctt
    16681 ttcttttccc tttgcaactc ttcaaaggtt agttcatgtt ctgaacgttg ccgaatatag
    16741 ttagctctat attcttcttc ttgagcggac aaggcatagt actctttatg gtaagcttgc
    16801 tctaagtcgt catgataaga acctaaaata cgattgagcg tcatcatatc tgagctatca
    16861 ttactatatc tcaaatcata ttcaacagcg tcgtaagttt cttgaagcat attaataatc
    16921 ttattttttc ttactgagag atcgtctaat ttttcttcaa actggcaaca actatggtga
    16981 taggcatcat ctaaatctga tatttttctc tgagtatcag ctaattgttt ttcaatgata
    17041 tcaatcagtg ccattgttta aactcccctg acaagttact atcttgtcgc aacatagctt
    17101 caactccttt attaatttgg ttttctaatt caacgtaagc gtgagaaatg ctttctgccg
    17161 cttgagtttt atttgagaaa aaggtttgag cttctccgac aatgctcgca tagctaacac
    17221 ctgactgagc atacgcttct tccaattctt gaggacttag ttcagtaact cctgtaggag
    17281 ctacttttaa agattgataa agctcttctg ctttagcaac tgctttttgc tcaacttttt
    17341 taacttcttc aactgcttct tttgatatcg ttaccaaacc actagagatc acttttgctt
    17401 gttgactatc taggaaaata gcttgattct tactaacttc ttttcccgta cttttaaagc
    17461 tactaagttt cgctttaata gcatgcattc tttgattcac attcgtatga actactgaat
    17521 agttttcttt gtaagcaaat ttatcttctg ctaattttaa agaaccatct ggattgtact
    17581 ggtagcctcc ccaaatatgc tgattggtaa aattagcaac ccagctacct cctgaaacag
    17641 ctttactgtc aactatttta acaattccga cagcattatc gcttccttct ttaggataac
    17701 ctattccaat tttatccttg gaatcaacgt aattattgac cacccctttt attttatcaa
    17761 cctgttcttt ttgtttatct gtcaaaatac tataaaggtt tggaccgtta tatatccaag
    17821 ctccttgaac tcggtaaatt tgattatcat ccaattctgc aatagcatac tgtgcattca
    17881 ttgaccctaa cgagtgtccg tagaccgaaa tttttgcgtt aggatattta ttcataactt
    17941 cttttagcag atgagctgat tgttctaact gctttgtacc ttgtttatga gatacacttt
    18001 gtgaaccaaa tttctttaat aatagtttac tagctattgg aatatcattt ttaaaccagt
    18061 ctgttttcca atcatcccct ccaaacggtg ctgttgaccc cttaaataaa accgtaactt
    18121 ccttaacatc ctctggcttg tctaaagttc catttggagt gatggtataa atttgttccc
    18181 cggtttcttt gcctccatct tgaacgtgag caactttacc gatggggagt accttatctt
    18241 ggctcattat tttgactagt gtatcaagtt ggctacctaa ttcatactct tgcttcgcta
    18301 tatttaccct atctaaatca gtcaagactt tttccattaa tgacctctac tttccaataa
    18361 atgagttaac tggggagatt gatggctact ttgactgacc aaatgccctt gattatccct
    18421 atccaaatta caagtaatat ataagttagg atctttatta atgattaacc ttaccgaaat
    18481 ccctcccata ggattttttt tgatactttc tttgtcaata tcatacgact taataacagc
    18541 attctctttg aaagcatctt catcttctgc ataaatccac ttttgataag catttttagc
    18601 gtctttgcta ttagctattt cccataattc ttgttgttta ttgttttcag tcacttttaa
    18661 acctcctaca gtgaaaccaa gccctaaaag gattattaca ataaaactaa gtaattttat
    18721 tgacttcatc tctcctcctt ttatcatata acactcattt gatatctagt ttatcataaa
    18781 tttattctca ttataaatga aaaactataa aacatggatt agttatctta ggattaccaa
    18841 atacaaagat attaaaaaag atgattaata gctctaatgc tataaatcat ctgactctac
    18901 tatttacttg taatcacttg gacgttctgt atattttgcc caagatttct tcaaaaattt
    18961 attattaatg tgataattcg gcttgctgtt ttcctctgtt aaaaatggat tgatatgctt
    19021 atctagctct aaccaaccat tccaacctaa atggattgta tcttgcataa agtatggctt
    19081 acctccatct cgagaaaggt cagcgatatg attgaaacct tgactttgaa gttggtgttt
    19141 tattttttct acagattttt gatacatttt ttgatcaagc cctgtgtagt ctgtccactt
    19201 tttatttact ggtggtatga caaaaaggac atctgtattt tgttttgaga attcattaag
    19261 aaccaactgt aaatcattaa attctggtga cttagtataa ttgaaatgtc gttgtgaccc
    19321 tttcaatttt tttaagtgct ttttaattct tgtttggtaa aagcgatcat caataccaaa
    19381 ttgatttgat ctcgttgcta ctttagcatc ttgtgatgca atacgcgata aatgattata
    19441 tgagaatgct ttcggtaatt taccaacatg aggcattatt gcctgattat aagtttttcc
    19501 tagtggtaga tagctaaaaa tagcatctgt cttctcaaat acccaatcat taaactttag
    19561 tctttgtttg tctttatttg atagtttttg gccatctgca actttttcta tcaaatctga
    19621 catagaagct tcaggatata attttaacaa tcgacgagca gcatacctat cgtaggttgt
    19681 ccctgtttga ttttgcaaaa atcgaatggt ttgatcgttg ctgaaatagt tttgaaaagc
    19741 acctttgttg gctcccttgc gaacaaacca ttgcggagaa ataacataga tagctttttt
    19801 atttttgatt tgtcctttaa tctgttgaat gccatagtat tgtgtcagag aagccgcccc
    19861 tttttgacct aaaagatatg ggatataaga cctattgtaa gcctctgcta aaactgatgg
    19921 atgcattgcg tcaaatctta gccactcact tgaaccaaag aaagggacaa aattggcttt
    19981 aggatcactg aaagctctaa ctttttgaac tctgctttta aaacctgcct tcgtcaacgc
    20041 tactgcactg tttttttcct tacctaaatg atgaggctga gaaacaggaa aaacaaaata
    20101 taatcctacc aacaatagta aagcacaaac tagaggtcca aatactttac ctagacgttt
    20161 aagcattgca gagctccgtt actccagcaa caattttatt agctgtgttc cagtcattac
    20221 gaccaaattc cgcaatcgga atatcaatat taaagtggct ttccaactca acaattagtt
    20281 caacagtacc catgctgtct agaactcctg cgtcaaataa gtcttcatcc atcatagaag
    20341 aaacatcctc cataaatagg tcatcaataa ttgctaatac ttctgattta atatccatta
    20401 taatactcct tattatttgt tatgcggttg tgggaaccac aaaagatcta aaaatcctga
    20461 aaaaatcaag aaagaaaaca taactacatt aaaggtgatg aagacaccca aagcataagt
    20521 ccatttgttt tctggtaaaa gtggtttttt agccaatctt ctttctttat taatattttt
    20581 cttcttacgt acccaagcgt cattaataac taggccaatc ccatgaaaga gaccataggc
    20641 tatatagtac caagttaacc catgccagaa tcccataaga agcatattga taatataagc
    20701 tacacttgaa gtaacattac ggtttttaaa aactttattt ttaactaaaa gcttaacaag
    20761 cctcatgaaa acaaagtctc taaaccagaa agaaaggctc atatgccatc tattccaaaa
    20821 ttcttttagg tcgcgtgatt tgaaaggttt gtcaaagtta atcggagact taatccccat
    20881 taggttagaa atagccaacg caaacattgt gtaacctgca aaatcaaaga acaaatcaaa
    20941 accaaaggca tacataaccc caagtgttgg ccaattaaac acaccaccct gttctagcgc
    21001 taattctttt agaggaggca taatcagact tccaaaaata taggctaaaa taaacttata
    21061 gagaaaacct aacatcaaat aatgaatcgc ttgacctaac atatctagga gttcgtttcg
    21121 atctgggata ttaatgtaat cctcattgaa tcgtttaaaa cgatcaattg gtccacttga
    21181 gaaagttgga aagaagagta aaaatcttaa gaattcccat aaagtaaatt ctttcaagac
    21241 accgtctctc atttcaataa tcatagcaac agctctaaaa gtaaggtaag aaattcctag
    21301 aaacccaaaa agtgactgat gcccatcctc agatatagct ggaattaatt ttaccaaaaa
    21361 tagcggaaaa atagataaac aaaccgttac ataaaagacc aaagtatgat taaatcgttt
    21421 ccggtaagct ttatagataa aaactagcag agactgtccg acaacgtatg ccaatagtga
    21481 cttcaattga ttaaaagtac taccagtcaa catcattaca ataaaactta gactaaaaat
    21541 aatctcatat aaggcaaaac gcttcttaaa gaataagccg atgtaaattg gtaggacagc
    21601 taagataagg tagaagaaat attgtggatt cccataaaca tccaagtgag gaagtttttc
    21661 taaaaatgcc tgtatcactt gtttacctcg ctcataagac ctttgatatc aattttccca
    21721 tttggtgtca aaggtaaatc ctctcgatag ataaatttag atggcatcat gtaatccatc
    21781 ataatgtcct ttaagtcttc cttaattgct tttgtcaaat ccaaatcacg ttcaaaatca
    21841 tctcttacac cttcttttaa gacaatatag gctaataagt tttgaacttt atgatccttg
    21901 ttataacgtg gcactgctac tgctgatttt acatactgcg atttgtttaa gttttgagaa
    21961 acatcttcta attcaatacg atagccgtta aatttaattt ggaaatccat acgcccaccg
    22021 taaagcagaa gaccttcatc ggtcatactt cctaagtcac cggtatggta agcaggtaga
    22081 ccttcgaatt ggaaaaatgc ctcagctgtc ttttctggat tgttaagata gccttttgat
    22141 actgctggtc cagcaataat gatttcacct tgctctccgt ttggtaattt atgaccttct
    22201 tcatcaatca catacgttgg tgagtcatct ttagtgtaac caattggaag gcgtttgcat
    22261 gtttctaaca tttcatcagt aattgctact gcggatagag caactgttgc ttctgttggc
    22321 ccatatgcat taacgatacg agcttttgga aaacgttgac gaagtttttg tgcagtcttg
    22381 acagttaact cttccccatc aaaataaaaa tgtgttaact gtggcaaggt ctctgaattg
    22441 aaatcgttag atagtagcgc catatcagca aatgatggtg tcgaagtcca aacctgtatt
    22501 ggcaattcat taatggtagc gaatagtttt ttgaaatcat taactactgt ttttggtagg
    22561 gcaaacaggg tgcctcccat agctagtgtt ggtgcccaat acataactga taagtcaaat
    22621 gaatatggcg gttgagccaa catttgcggt ctttcaggaa ctgaaaactc atcatcagaa
    22681 atcatccaat ttgtaaagct caataaattg tcatgtgaaa tttgcacacc ttttggtaaa
    22741 ccagtagtcc ctgaagtgaa aataatatag taattatcat cacctttaac agaatgtgtt
    22801 acctcataag gagtcttttc ttcaaaaata gctgaaactt gagaaacgtc taggattggg
    22861 acattatcaa cttcaagagg aaattcacca attgaaatga taaggcttgg ttgagcaact
    22921 gtcataatag cctgtattct atccaaagca gagtgttggt caaccggtat ataagcatgc
    22981 cctgactttg ttaaagcaac aaatgtcgcc aacatttcat attcttgacc accgaatact
    23041 aagacaggtg atttttcaac aaggcctagg ctatcaatat gagcagctag agagtcagag
    23101 tctactttaa gttgtccata agtatggact tcccctaaaa tatcatacac tggaaaatca
    23161 gcttgtgtct cagcaaaatg ctcaattgtt ttaatcatat catgtatcat ttgtaccatc
    23221 tcctagaatt cattataaat aaaactgcct tgtccgcgac caaggtatgc aaaaaagtaa
    23281 attagtaaca ataaaatacc gaagtataat aacgttctac caataaaaga taaatatggt
    23341 ttcatcattc ttcctttaat taatcatggt attggtccaa aatccatcaa ttggtaactg
    23401 gacttcaaga cgtaattggt ctttttcttg atatgctcta attctcccct tatggcgttc
    23461 cacgattgct ttagcaatag ataagcctag accaaaactg agagaatctg catgatcatc
    23521 tttagcttga taaaaacgct caaagagatt atccataacc tctttggaat agatagcact
    23581 tggatttgat attgtcagaa tagcttttct tcgagcttgt ttaagactaa actggattgc
    23641 tgattttgac aaagaatgcc taatagcatt atccaaaaga attattaaaa tttgcttaat
    23701 ctgactctct tctccaacaa tccagacatc atcttcaatc gtatcattta aacacttttc
    23761 ttcttcatat gccaaacttt catatgttag aagtacttct tgacataaga cagataagtt
    23821 taactttttg aaatttacct tggagcgttt ttcatcaagt ctagacaaat taagcaaatc
    23881 ttcaattaaa aactccattc gcttagtctc cgaaacaaca tgatccagat aacgattccc
    23941 tggaatttgc tgttccagaa cctgaacgtt ggctcgtata gctgcaatag gggtcttcag
    24001 ctcatgactc gcatctgaaa cgaactgttt ttcgcgagtc atttctgtct ctacaggtcc
    24061 tacaataaat cgagaaagat aaagacttac agctgctaat aaacaaaatc caaaaatagt
    24121 aaaaacaaga attagaaaac gccttgcata agggacttca taactagcca aatccataat
    24181 agctatggtg taggttttat ctttatattc ttttatggaa tatataaaag acttccattt
    24241 tcgtgacttc tttccttgct caaggagctt caaactagat ttttcgataa catcatttgt
    24301 caaatcagta ttattgcttt gaatcttaac catcttttgg ttatctatag tcaccaaagc
    24361 aatcggttca tcgcctggta ttcctaaata atcatttttg gcaactaatt taacaatcct
    24421 atatgcatca tattcatccc aatattgatt gtagcgatta ctaactgcaa atagaaagag
    24481 aacaacgaca ataaggattg acatggtcaa aaagacaaat tttttgcgta aatcactaaa
    24541 cattatgtcc tctcctccaa ggaatatcca aagcccttag tggttattat ttcaatgtct
    24601 gcttttaaaa agcgcaattt ttttcgcaaa aaagaaataa atacttctaa ctggttatat
    24661 tctgaattgt ctgaaggtcc ccaaacctta gaaatcaact gatttttagg aataatctgc
    24721 tttgcatttc gcataaaaac ttccatcaag agaaattctt tattggataa ttttacacta
    24781 ctttctttag attctagctc atgtgaatct gtgttaagcc ttatatttcc aagccgcaat
    24841 tgatttgctc tgattagaga agattgtctg gttctcagcc taattctagc tagcaattca
    24901 tctgcttcaa agggttttgt gatataatca tctgccccta aatctaaacc attaacttta
    24961 tcataagtct gtgattttgc agttaaaaag attattgggg tttctaaacc agcttccctc
    25021 actcttttta aaacagataa tcctgagcgt ttgggtaaca taatatccaa caggactaaa
    25081 tcatagagac ctgtcaaaat gtattctaac ccatcatctc catcaaatgc taaatcaaca
    25141 gaataacctt tatctgccaa taaagcctga atagcctctg ctatactttt ttcatcctca
    25201 actacaagaa gtctcatttt ctagtcacct taccttatat attatagtat aaactagcaa
    25261 aattgaaata accttgaaaa aacgatatta ataaaagtat ttcctaagtc actaaactac
    25321 ttcaataata caagcgtcta aaaaataaaa tatcactcgc tataaagata tttctcaata
    25381 agaaatctcc tcatattcta acaaaaatag gggctatttg tctacgaatt tccgcattat
    25441 aaactcactt ttcattaact ttatctataa tatactcaaa ctactctcac aaaaaccttt
    25501 atcgttatga aggtaccaaa aaagccacca gactcgagta cctgttggct tgatattatt
    25561 ttgctatcta tattaagctg ataacacttt acgtccttta cgacggcggc tagcaagcac
    25621 gcgacgtccg tttttagttg acatacggtg acggaatccg tgtttacgtt ggcgacggat
    25681 tttacttggt tgataagtac gtttcacgtt gaatacctcc tgatagattt tcgtattcgt
    25741 ttagccggct attttgatca gttactaaac ataccatact attttataac aattttaggc
    25801 ctcttgtcaa gaaatacttt caagttcatt tgtctttttt cagtaaatag ttctatttgt
    25861 tagcaagaaa tgcttctcta ttttctccaa ctaaaagaaa agatgtgcca tcaaaacaat
    25921 aataggtaat gaaataattg ttctctctat aaaaatgata aataattcta gaatgttgat
    25981 agggatcttg gaacctaata tcaccgcacc agtctcagac atataaatta attgcacaat
    26041 agaaactgtt gcgacaataa accttgtcat ctcactgtga atttcagccg ccattatcga
    26101 aggaacaacc atatctgaaa aaccaacaat catggtttga ctcgcaatat ctgcttctgg
    26161 aatccctaaa agttcaagga acggaaggaa tggtgtacct aaaattttaa aaataggtgt
    26221 tgatactgaa ataatcaaag ctattgttcc gacagtcata attgttggca taacgccaaa
    26281 ccataaacta agtactgtct tagaggcatt attgacaaaa gtcttcatgt ttccatttgc
    26341 ttttgattta tcgatggcaa gcttaagtcc ccactctatc ttgttataac catccggaat
    26401 tacctcagat gtttctttct ttggtttagt gtaataacta tttgattgac gagataaggg
    26461 agggatataa gggataatga atgcacagac aacactgcta atgatgactg ttagataaaa
    26521 atggccaaaa taatcagtta agccaacatt ctgcaaaaca acaagacaaa aagtgattga
    26581 tactgctgaa aaagtagtgg aaatcgttgc agcttctcta ctggtataat aaccctcaac
    26641 atactgctta tcggtcaaag caattccaat agtaccatca cctacaaatg aggctacaca
    26701 attaactgct gaccgaccag gtagctgaaa gaatggacgc ataatgggag ttaggaagac
    26761 accaacaaac tccagtagac caaattctgt caaaaatgga agaataaaac ccgcagcaag
    26821 gaaaatggta aacaaaccac caatcaaatc aaaaaggata aggccgcctg tctctttccc
    26881 acataccatt tcaggaccaa tatgtaaaac cgtcatcaga ccaagtgcca aaccgatcaa
    26941 acgaataatc agccagaaaa tggtaatgtc agaaatttct tttagcaagt catttttttc
    27001 aataaagcta ggtctaaaca gacgataaca aagtgctaac acacatgaaa taaaaatgat
    27061 tagtaaaata atataatgta taggaaaaac agcgttaatc cattgattaa ttgtcttaga
    27121 gattacagag acagctactg ttgtcatacc attatattta aatggagtca ttaataataa
    27181 tactccaaat aacgacggaa taataaacca caagatatct ttaatagata tactttcttt
    27241 tttcatacaa tcctcctgga taatatggaa ttgtttctaa atatactcat aacttccatt
    27301 gtaaaatttt ataataaaaa aaacaacctt gaattaaaga aaattttact tagtaatact
    27361 t
//
LOCUS       DATRNX010000013        27326 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRNX010000013 DATRNX010000000
VERSION     DATRNX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27326
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            127..1617
                     /locus_tag="VBC81_000461"
     CDS             127..1617
                     /locus_tag="VBC81_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6911523.1"
                     /translation="MGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKK
                     YKISKDGLTWSFDLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGK
                     NQVNIHLTEAHSTFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVR
                     NPYWHGKKPYFKKWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASN
                     DVRGLSLPYVKKGVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKP
                     AYSIIDRTPFWNPKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTN
                     DQLRANLAVEVAEQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHY
                     PSLAGKGWTNITFYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPN
                     VWLVSLNHTYIGDKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            1730..2707
                     /locus_tag="VBC81_000462"
     CDS             1730..2707
                     /locus_tag="VBC81_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911524.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            2704..3525
                     /locus_tag="VBC81_000463"
     CDS             2704..3525
                     /locus_tag="VBC81_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911525.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            3537..4340
                     /locus_tag="VBC81_000464"
     CDS             3537..4340
                     /locus_tag="VBC81_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911526.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            4324..4950
                     /locus_tag="VBC81_000465"
     CDS             4324..4950
                     /locus_tag="VBC81_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911527.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            5232..7262
                     /gene="treP"
                     /locus_tag="VBC81_000466"
     CDS             5232..7262
                     /gene="treP"
                     /locus_tag="VBC81_000466"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6911528.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            7484..9109
                     /gene="treC"
                     /locus_tag="VBC81_000467"
     CDS             7484..9109
                     /gene="treC"
                     /locus_tag="VBC81_000467"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6911529.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            9325..11361
                     /locus_tag="VBC81_000468"
     CDS             9325..11361
                     /locus_tag="VBC81_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6911530.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLTAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETISLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            11364..11648
                     /locus_tag="VBC81_000469"
     CDS             11364..11648
                     /locus_tag="VBC81_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6911531.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            11661..13016
                     /locus_tag="VBC81_000470"
     CDS             11661..13016
                     /locus_tag="VBC81_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6911532.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            13019..13876
                     /locus_tag="VBC81_000471"
     CDS             13019..13876
                     /locus_tag="VBC81_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6911533.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            13873..14802
                     /locus_tag="VBC81_000472"
     CDS             13873..14802
                     /locus_tag="VBC81_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6911534.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            14911..16170
                     /locus_tag="VBC81_000473"
     CDS             14911..16170
                     /locus_tag="VBC81_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6911535.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            16258..16527
                     /gene="rpsO"
                     /locus_tag="VBC81_000474"
     CDS             16258..16527
                     /gene="rpsO"
                     /locus_tag="VBC81_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6911536.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            16907..19036
                     /gene="pnp"
                     /locus_tag="VBC81_000475"
     CDS             16907..19036
                     /gene="pnp"
                     /locus_tag="VBC81_000475"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6911537.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            19038..19790
                     /locus_tag="VBC81_000476"
     CDS             19038..19790
                     /locus_tag="VBC81_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6911538.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            19799..20383
                     /gene="cysE"
                     /locus_tag="VBC81_000477"
     CDS             19799..20383
                     /gene="cysE"
                     /locus_tag="VBC81_000477"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6911539.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            20393..20575
                     /locus_tag="VBC81_000478"
     CDS             20393..20575
                     /locus_tag="VBC81_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911540.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            20572..21915
                     /locus_tag="VBC81_000479"
     CDS             20572..21915
                     /locus_tag="VBC81_000479"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6911541.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            21908..22294
                     /locus_tag="VBC81_000480"
     CDS             21908..22294
                     /locus_tag="VBC81_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6911542.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            22397..23152
                     /gene="rlmB"
                     /locus_tag="VBC81_000481"
     CDS             22397..23152
                     /gene="rlmB"
                     /locus_tag="VBC81_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6911543.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            23149..23667
                     /locus_tag="VBC81_000482"
     CDS             23149..23667
                     /locus_tag="VBC81_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6911544.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            23760..24620
                     /locus_tag="VBC81_000483"
     CDS             23760..24620
                     /locus_tag="VBC81_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6911545.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            25157..25279
                     /locus_tag="VBC81_000484"
     CDS             25157..25279
                     /locus_tag="VBC81_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6911546.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            25501..25947
                     /gene="rplM"
                     /locus_tag="VBC81_000485"
     CDS             25501..25947
                     /gene="rplM"
                     /locus_tag="VBC81_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6911547.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            25968..26360
                     /gene="rpsI"
                     /locus_tag="VBC81_000486"
     CDS             25968..26360
                     /gene="rpsI"
                     /locus_tag="VBC81_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6911548.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            26815..27105
                     /locus_tag="VBC81_000487"
     CDS             26815..27105
                     /locus_tag="VBC81_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6911549.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
BASE COUNT         8681 a         4348 c         5576 g         8721 t
ORIGIN      
        1 ttgaagtaca tctcttttgt tgctttgttt ctggcgagta tctttttagt agcttgtcaa
       61 aatcaaaatt cacaaacaaa ggagcgaaca cgaaaacaac gacccaaaga tgaattggtt
      121 gtctctatgg gggcaaagct tcctcatgaa ttcgatccaa aggaccgtta tggaatccat
      181 aatgaaggta atattactca tagtacccta ttgaaacgtt ctcctgaact agatataaaa
      241 ggagagcttg ctaaaaaata taaaatctct aaggatggct taacgtggtc gttcgactta
      301 aatgatgatt ttaaattctc caatggtgag cctgttactg ctgacgatgt taagtttact
      361 tatgatatgt tgaaagcaga tggaaaagct tgggatttga cctttattaa gaatgttgaa
      421 gtagttggga aaaaccaggt aaatattcat ttgactgagg cgcattcgac atttacagca
      481 cagttgacag aaatcccaat cgtccctaaa aaacattaca atgataagta taagagcaat
      541 cctatcggtt caggacctta catggtaaaa gaatataagg ctggagaaca agcaatcttc
      601 gttcgaaatc catattggca cggtaaaaag ccttatttta aaaagtggac ttgggtatta
      661 cttgatgaaa atacagcact agctgcttta gaatctggtg atgttgacat gatttacgca
      721 acgccagagc ttgctagcaa gaaagtcaaa ggaacacgtc ttctagatat tgcttcaaat
      781 gatgttcgtg ggttatcgct accttatgtg aagaaaggtg ttgtgaaaaa ttcaccagac
      841 ggttatccag taggaaatga tgtcactagt gatccagcaa tcagaaaagc cttgactatt
      901 ggtttaaata gacaaaaagt tctggatact gttttaaatg gttatggtaa accagcttat
      961 tcaattattg atagaacacc attctggaat ccaaaaacag caattaaaga taataaagta
     1021 gctaaagcta agcaactttt gacaaaagct ggatggaaag aacaagcaga cggtagccgc
     1081 aaaaaaggaa atcttaaagc ggaatttgac ctttactacc ctactaatga tcaattacga
     1141 gcaaacttag ccgttgaagt agcggagcaa gctaaagcct taggtattac tattaaactc
     1201 aaggctagta actgggatga aatggcaact aagtcacatg attcagcctt actttatgcg
     1261 ggaggacgtc atcatgcgca gcaattttat gaatcacatt acccaagttt agctggtaaa
     1321 ggttggacca atattacttt ttataacaat cctactgtga ctaagtacct tgacaaagca
     1381 atgacatctc ccgaccttga taaagctaac aaatattgga agttagctca gtgggatggc
     1441 aaaacaggtg cttccactct tggagattta ccaaatgtat ggttggtgag tcttaaccat
     1501 acttatattg gtgataaacg tatcaatgta ggtaaacaag gtgtccatag tcatggtcat
     1561 gattggtcat tattgactaa cattgctgaa tggacttggg atgaatctgc taagtaacta
     1621 atttagttaa tgtaggctac tttaagtttg gagtcagaga aaacaatgta caccttatgg
     1681 tgtgaatggt ttcctcgact ccttttttaa caattaagga gatttatctg tgaaacatat
     1741 taccactgta gttatctgga agatacttag gtgtttgtca ctcgtttttg gagtatcaat
     1801 tttgaccttt attttactaa aacaatcacc ggttgatcca gtcatggcaa gtgtcaatta
     1861 tgacacgtca ctaacccctg ttcagtacaa agcgattgct caccactatg gcttggataa
     1921 gccagctccg gtccagtatt ttatttggtt aaaaaatttt atacagggac atttagggac
     1981 ttcactagtt tatcggcaac ctgttattga tattattaga tcacgggcag gtgcctcttt
     2041 catacttatg ggactctcct gggtcttatc gggtcttatt ggatttatct taggaacttt
     2101 atcagctttc catcaaggga aattgcttga tcgaattgta aggtggtttt cttaccttca
     2161 gatatcagta ccaacttttt ggattgggct cattttttta ttaatctttt ctgtccagct
     2221 ggggtggttc ccgattggta tttcttctcc gataggtact ttgagtcaag atattacgtt
     2281 agctgatcgt attaagcacc ttattttacc tgttttcacg ctaagtattc taggcattgc
     2341 caatgtaact cttcatacta gaactaaaat gatgtcggta ctttctagtg aatatgtctt
     2401 atttgccaga gcgcgtgggg aaacggaatg gcaaattttt aaaaatcatt gtcttagaaa
     2461 tgctatcgta ccagctatta cactgcattt ttcctatttt ggagaattgt ttggaggatc
     2521 cgttcttgct gagcaagttt tctcatatcc aggactaggg tctaccctaa ctgaagcagg
     2581 acttaaaagt gatacaccgc tacttctagc tattgtgatg atagggacat tatttgtttt
     2641 tgcgggcaat cttattgcgg atattttaaa tagcataatc aatccacagt taaggagaaa
     2701 agtatgatat tgagacgtcg aactattgtt ttatggcaac tgggtatcgc catttctctc
     2761 attcttagta ttctagcctt aaatctttat ttccatagta ctcccttgca aaccaatgca
     2821 gctttacgga accttgctcc ttcattaaac catctttttg ggacagatgg tttaggtagg
     2881 gatatgtttg tcagaacgat taaaggactt tatttctctc tacaagtcgg cttattaggt
     2941 gcccttatgg gggtcattct ggcgacagtt tttggagtgc ttgcaggttt aggaaatagc
     3001 attattgata aaataatagc atggttagtt gatttgttta ttggtatgcc tcatttgatt
     3061 tttatgattc tcatttcttt tgttgttggg aaaggtgctc aaggggtcat cattgcaacg
     3121 gctgttacac attggccttc tttagcaagg cttatccgca atgaagtcta tcatctaaag
     3181 aataaagaat ttgtccaact ttctaaaagt atgggaaaaa cgccttatta tattgtgagg
     3241 catcatatcc tgcctttgat tgcttctcaa attttcattg gttttatcct cttatttcca
     3301 catgtcatcc tacatgaagc atcaatgact ttcttaggat ttgggctctc tgccgaacaa
     3361 ccttcggttg gtatcattct gtcagaggca gctaagcata tctctcttgg aaattggtgg
     3421 ttggttatct ttccaggact ttatcttatt ttggttgtca atgcatttga tactatcgga
     3481 gaatctttaa agaaactctt ttaccctcaa actgatcatt tttaggaggt agagatatga
     3541 cagaaacatt attaagcatt aaagacctct ccatcacctt cactcaatac ggaagatttt
     3601 taaaaccatt tcaatcaaca ccgatacaag cgctgaattt agaaattaaa aaaggtgagt
     3661 tattagctat tataggtgct agtggttcgg ggaagagttt attagcacat gctattatgg
     3721 atattcttcc taaaaatgca tctgtaacag gagatatgat ttatcgtggt caatcactaa
     3781 attctaaacg cattaaacag ttgcgaggaa aagatattac gttgattcca caatcagtta
     3841 attatttaga tccatctatg aaagtcaaac atcaggtgcg cttaggtatc tcagaaaatt
     3901 caaaggctac tcaagaagga ttgtttcaac agtttggttt aaaagaaagt gatggtgact
     3961 tgtatccttt ccaactttct ggcggaatgc tccgacgtgt tttgtttaca acgtgtatta
     4021 gtgataaggt ttctttgatt attgcggatg agcccacccc tggattacat ccagatgctc
     4081 tgcaaatggt tttagaccaa ctacgctcct ttgcagataa aggaataagc gttatattta
     4141 tcactcatga tattgtagca gctagtcaaa ttgctgatcg tattactatt tttaaagagg
     4201 gaaaagctat tgaaacagct ccagctagtt tctttagcgg aaatggagag cagttacaaa
     4261 cagaatttgc tagaagttta tggcgctctc tcccacagca agaatttttg aaaggagtta
     4321 ctcatgacct tagaggctaa aaagcttggc ttttatcata aaaaagatca atggcttttt
     4381 aaggagatta atttagaggt agcacctggt caagttttag gtatatttgg acaaagtggt
     4441 tgtggaaaaa ctagtttatc caaagtgctt gcaggttttt tacatcctag atctggtgaa
     4501 gtattagttg atggcagtaa tttgcctaac aaagcattta gacctgtaca acttattcaa
     4561 caacatcctg aaaaaacgat gaatccttta tggcctatga aaaaaagttt agaagaagct
     4621 tattacccaa gtcgagattt actagatgct tttggaattc aagaaaaatg gctaaatcgt
     4681 cgccctagtg aactctcggg aggggaatta caacgctttt cgattgtgcg ttcattacat
     4741 ccagagacta aataccttat tgcagatgaa atgactacta tgttggatag tattacacaa
     4801 gctagtgtat ggaaaagcct gttggagatt gtaaaggata gaaatttagg tctcatcgtt
     4861 attagtcatg atttcgctat gttagagaaa ctttgtaacc aatgctatat gattgaagag
     4921 aatcgtatcg tatctttcaa tggagattaa aaaactcacc tactatatca attagtaggt
     4981 gttttttatt aagagagtat tgggggtgac tgacctactc atgactctaa cattttggta
     5041 acgttggtga gaaattctaa tgttttgggt attattgact aattatttta taatttatag
     5101 attttttaaa aagataagct aaggatttgc atagggatat gtaatcgttt acaattatat
     5161 tgtaagctag tcttctttaa gtttctgcct tggagtcaca gactctccgt taaatagata
     5221 aggataaaca gatggaacaa tttaaacatg atgccaaagc cttgttagag gcaattgggg
     5281 gtaaagaaaa tattagtgca gtgacacatt gtgccacacg catgagattt gttttgaatg
     5341 acagtagtaa agctaaggta aaggtaattg aggagttacc atcagtcaag ggtaccttta
     5401 ccaatgctgg gcaatttcaa gtcattattg gtaatgatgt ccctattttt tataatgctt
     5461 ttgtagccgt ttctggtatt gagggtgtgt ctaaagaggc ggcaaaatcg gctgcccaaa
     5521 aaaatcaaaa ccctttacag cgtgtgttga caatgctggc tgaaatattt acaccgatta
     5581 ttccagccat tattgttggt ggtctgatcc ttggttttcg taatatttta gatgccgttc
     5641 catttgaatt tttaggtcaa aaagttgttg atggtgtcag gcaagtagac tcttctgggc
     5701 atccaatttg gaatactcta gtagatgttt caacgttttg gagcggagta gactcttttt
     5761 tatggttacc aggtgaagct atatttcatt tcttaccagt tggtattgta tggtctgtga
     5821 ctcgtaagat ggggactact caaatcctcg gtattgttct aggtatctgt ctagtgtcgc
     5881 cacaattact taatgcttat tctgtagcta gtacttcagc agcagacatt gctaaaaatt
     5941 ggtcttggaa ttttggttac tttacagtac aaaaaattgg ttatcaagct caagttattc
     6001 cagctctgtt agctggtttg agcctgtctt acttagaaat cttctggaga aaacatattc
     6061 ctgaagttgt ttcaatgatt tttgtgccat ttctatcctt agttccagct attatcttgg
     6121 ctcatactgt ccttggacct attggttgga cactaggtaa atggatttct gctatcgttc
     6181 ttattggatt gactggtcct gtaaaatggc tctttggtgc tatttttggt gccctgtatg
     6241 ccccatttgt tattactggt ttgcaccaca tgacaaatgc tatcgatact caactcatag
     6301 cagatacaaa aacacataca actggccttt ggccgatgat tgctctttca aatattgctc
     6361 agggttcagc tgtactagct tattatttta tgcatcgtca tgatgaaaaa gaggctcaaa
     6421 tatcacttcc agctgctatc tcagcctacc ttggtgttac agagccggcg ctttttggtg
     6481 taaacgttaa atacatttat ccatttgttg ctggtatgat tggttcaagt gtggcaggtc
     6541 ttttggcgac aacttttaat gtccaagcta actctattgg tgtaggcggt cttccaggct
     6601 tcctgtcaat taatgttaaa tatatgggat atttctttat ctgtatggca gtggctatct
     6661 ttattccttt atttttgaca ctattcttta aaaaatcagg tattttgaca aaaactgaag
     6721 aggaaaaatt agtatctgat gctgtcattg cttcaacaac agaaacaaaa tcagcaaaag
     6781 aaaaagctgt cgtctcaggt acaaagctta gtgtagtaag ccctttatca ggtttagcta
     6841 aaccactgga tcaagctagt gatcctgtct tttcacaagg aattatgggg aagggagtgg
     6901 ttatcgatcc aagtgatgga gaactagttt caccggtcga tgcgacagtt tcagttcttt
     6961 tccctacgaa acatgctatt ggattattaa catcagaggg tgttgagttt cttattcata
     7021 ttggtatgga tactgttaat ttagaaggaa aagggtttac ctctcatgtt gcccaaggtg
     7081 acaatgtaaa agtaggagat aaactcatta ctttcgatat tccaatgata aaagaagaag
     7141 gatacattgt ggaaactcct attctgatta ctaatcaaga agaattccga ccagaagaat
     7201 tgatagattt acccaaacag ataaaacgtg gacaagcatt aatggttgcc aagaaaattt
     7261 aaaagtaatt aggtatcctt tttaaaaaag agcactctta taaaatgaga ggtaatttga
     7321 ggaattgttt gaggggctta atacattagc tccttgaatt attttgaaca taagaaaaaa
     7381 ctcatgggaa caaatatcgc tgtaaaaatg ttatattttt gatgatagtt gatgttatag
     7441 ttagactgct aaatacagca gcattttggg aaaggagcag aatatgacga ttgataaacg
     7501 taaagttgtc tatcaaattt atccaaaatc atacaaagac acaactggta atggggttgg
     7561 tgatcttcgt ggtattatcg aaaaattacc ctatttagca gaacttggta ttgatatggt
     7621 ttggctcaac ccattttatc caagtccaca acgagataat ggatatgata tctctgatta
     7681 tacagccatt aaccctgatt ttggaacgat ggacgatttt gaagaaatga ttgaggtggg
     7741 acgtcaatac cgtatagatt ttatgcttga tatggtgctt aatcattgtt caatagaaca
     7801 tgagtggttt aaaaaagcac ttgcaggaga tcgctactat caagatttct ttatcttacg
     7861 tgataatcca acagattggg tatctaaatt tggtggaaat gcttggtcac catttggtga
     7921 tacagggaag tattatcttc atctttttga tatcactcaa gctgacctta attggcgcaa
     7981 tgccgatgtt cgcaaggaac tttttaaagt tgtcaatttt tggcgtgata aaggggttaa
     8041 aggttttcga tttgatgtga ttaatttaat tgggaaagat gagatacttg agaactgccc
     8101 aattaatgat ggaaaaccag cttatactga tcgtccaata acacatgatt atctcaagat
     8161 gttgaataat gctagttttg gacaagctga ttccttcatg acagttggtg aaatgtcttc
     8221 aacgacaatt gcaaattgca ttctttacac tgcaccagaa cgtgaagagt tatccatggc
     8281 ttttaatttt caccacctca aagtagatta taaagatggg caaaagtgga ccattatggc
     8341 ttttgatttt cctgcattac gtgacctttt ccatagttgg ggcgaaggaa tgagcgaagg
     8401 aaatggttgg aatgctttat tctataataa tcatgaccaa ccgcgagcat taaatcgttt
     8461 tgtagacgtg aaacgttttc gaaatgaagg agcaaccatg ttagcagcat ctatccactt
     8521 atcgcgtggt actccttata tatatatggg tgaagaaatc ggaatgcttg atcccgatta
     8581 cagttctatg gatgattatg tggatattga gagtttgaat gcctaccaaa taatgcttga
     8641 tgaaggcaag tcacaagaag aggcatttag cattattcga gcgaaatctc gagacaattc
     8701 tcgagtacca atgcaatggg atgatagtac gaatgcaggt ttttctgaag gcgctccatg
     8761 gttgaaagta ggaaaatcat ataaagaaat caatgtcgca aaggagaaaa cagggctgat
     8821 atttactttc tatcaagagt tgataaggct acgtaaacaa ctgccaataa tagctgacgg
     8881 gaattataaa gcagctttca aagataacga aaaagtatat gcttttgaac gccatcttga
     8941 taaggaaaaa cttcttgttt taaataattt ctttgctgag aaagttaaga taaaattacc
     9001 ggagaattat ctccaaggac aagttcttct atcaaattat aaggatgtta ctcttgatga
     9061 aacggttact ttgcaacctt atcaaacttt agctatctta gtgtcataac atttaaaaaa
     9121 cctttactta ttcaattaag taaaggtttt ttaagtgctg cttgtgggaa ggaatcttgc
     9181 tctagatttt gcaatttctt tcttttttct ttactctata acaatagccc aaattcattc
     9241 aattgatagt agtgccagat tgggctcaaa tgacctagaa aaatgtaaac ggttactata
     9301 aaataaaaat agaaaggaaa acaaatggtt gataataaga ctgttgtgat aatgttggta
     9361 tttttagctc ggaaaaatct ttccttgtat gagttaacag tgcaaacaaa gttttcaata
     9421 aaagttatca tagagcaaat taactatctc aatagttttt tagcaaaaaa tcacctaaca
     9481 gctattgctc actctgctgg acgttatcag ttattaggag atgaaaaaga gcatgataaa
     9541 attgtctcct tattagaagc tgagcaattt tacctaactc aggaagaaag ggtatgtctg
     9601 atttaccttt atagcttttg ccgaagagag tttgtctcca atgtgcatta tcaagatttt
     9661 ttgaaagtaa gtaagaatac tactttatca gatatcaaga tgctacgttc taaattagca
     9721 aaaagaggta tctcactcac ttatacaaga gcaaagggat atagtctagt aggagatgag
     9781 atggataagc atcaagttgc ttttcagatg ataactcaac tactagaatc tcccattggt
     9841 ttttggtctt tgaattatat cttgtcttct tggaaattcg ctctctcata tgagaagtta
     9901 gaaaagacgg tagaatattt ctatgaatct tttcaacttt ctccaataca agataggctt
     9961 gagaaatctt tatattttat cattttaata ctgtgtcgtt atcaaaggtc agtagatcgt
    10021 gttttgcagg gttctcctat tgtttctgaa caattaaaag agttaacgac tattattgtg
    10081 actaatttaa gtcaggatat ttccttatct aaaccattag atcagaaaga aaaagattat
    10141 attaccttga tcttatctgg ctgttttgaa ggtgagggaa ctaaagatga tgatttcttt
    10201 gaagcattgg caaaagcaat cgttgatgag atggaaacta tttcacttct taatttttcc
    10261 aataaagaag agttattaca aggtctgaag cgtcatatta ttccagctta tttccgattg
    10321 aaatatggtt taactggcga tagtggttat acacaaaata ttaaagagca ttacagtgat
    10381 ttatttctat tggttaaaaa ggcactacgt cctttggaag agcaagtagg attaataccc
    10441 gatagtgaaa tttcttactt cgtgattcac tttggaggct atcttagaca atcaggtgat
    10501 acacaatcaa tgtcttataa agctttgata ctctgtccaa acggcgttag ttcatcctta
    10561 gtgattaagg aaaaattgcg tggattattt cctcaaattc attttcatcg tgtctctaaa
    10621 attgagcagc tgaaactgat tgacaatcaa acttacgata tggtcttttc tactatattt
    10681 gtagaaacaa aaaaacctaa ttatctagtt tcgctgatga tgacagcaga gcaagtacaa
    10741 cagctcaaag aactggttat ttcagatttt cccaaagctt gccttgacga tttccaactt
    10801 gatcaattaa tagcaactat aaaaaaatac gctcatgttc attgtgaaga ggaattaaaa
    10861 ttggctttga ggacaatggt aaaacaagat atatttagaa aggatgtgag gcccttgttg
    10921 caccaactta taacagagga aacttatcag acaagttctg aacaaatgaa ttggaaagag
    10981 gcaattcgtt tagcggcaaa acccttgtta gcttctggaa aaattacgga aagttaccca
    11041 gaagctatga ttgaaaaggt cgaagagttt ggtcctttta tcaatcttgg taaaggtatt
    11101 gctattccac atgccagacc agaagatggg gtaaactctg tcggtatgtc aatgcttgtt
    11161 ttggaacagc ctatttattt attggatgac cccaaacagg aaatttatct cttattatgc
    11221 atagcagcta ttgataatga aacgcatttg aaagctctat cgcatttgac aacaatactt
    11281 cgtgataata ataatgttaa agctttgtta gcttcacgtg ggtatcaaga tattgaaatg
    11341 attataaaac aggaggatta aagatgttaa gaattggaac agcatgtgga tcaggtctag
    11401 gttcaagttt tatggtacaa atgaatattg agtctatttt gaaggatttg ggggtttctg
    11461 atgttgaagt tgaacattat gatttaggtg gagcagatcc aagcgcagca gatgtttgga
    11521 ttgttggtcg tgatttggag gattcagctg gtcacctggg ggatgttcgt attttaaaca
    11581 gtatcattga tatggatgaa ttgagagaat tagtgacagg tatttgccaa gaaaaaggct
    11641 taatctagga ggttgatgcc atgaaaggtc tattggattt tttagttaat attgccagaa
    11701 cgccagctat tttagtcgcc ttgatagcca ttatcggttt agtactgcag aaaaaaggtg
    11761 ttcctgatat tgtaaaaggt ggaataaaaa catttgttgg cttcttagtg gtttctggag
    11821 gtgcagggat agtccaaaat tccttgaatc catttggaaa aatgtttgaa catgcttttc
    11881 atttggtggg ggtagttcct aataatgaag ccattgtagc agtagctctt acgaagtatg
    11941 gctcagcaac tgctttgatt atgttagcgg gaatgatttt taatatttta attgctcgtt
    12001 ttacaaaatt taaatatatt tttctgacag gacatcatac attgtatatg gcttgtatga
    12061 ttgctgttat ttttgccgta gcaggcttta cttcgttctc gctcatcctt tttggaagtc
    12121 tagcgttagg gattattatg agcgtatctc ctgcatttgt gcaaaaatac atgattcaac
    12181 tgacaggtaa tgataaggtg gcacttgggc actttggttc tttaggctat tggttgagtg
    12241 gatttattgg tggaattgtt ggagataaat caaaatcaac agaagatatt aaatttccaa
    12301 agagtttatc atttttacgt gacagtaccg taagtattac tatctcaatg gcaattattt
    12361 accttatcgt tgcagtgttt gctggtgagg cttatattgc aaaagaaata agtaacggtg
    12421 tcaatggtct tgtttatgcc ctccaactag ctgggcaatt tgcagcaggt gtattcgtta
    12481 ttttagcagg tgtacgcctt atcctaggtg aaattgtacc agcctttaag ggaatctcag
    12541 aaaaattggt tccaaattca aaaccagcct tggattgtcc aattgtttac ccttatgcac
    12601 ctaatgcagt attgattggt tttatctcaa gttttgttgg tggattggtt agtatgatag
    12661 ttatgattgt gactggcact actgtcattt taccaggggt cgttcctcat ttcttctgtg
    12721 gggcaacggc aggtgttatc ggtaatgctt ctggtggtgt gagaggagcg actattggtg
    12781 catttgttca aggtatcctc atcagtttct tgccgatttt cttgatgcct gtactaggtg
    12841 gacttggttt caaaggttca acattctcag atgctgattt tggtttgacc ggtatcattc
    12901 ttggcgctct taatcatgtc ggcggtgcga tagctattgt tattggtatt gtagttatct
    12961 taattggact ttttggcatt tcatttgttg gaaaatcaac ccataaggaa ggttaataat
    13021 gacatcacca gaagaaagat attcatctct aagacgattt gcaacggaga ttcgtttaaa
    13081 tacactagaa acgctaaacc atttaggatt cggtcactat gggggtagtt tatcaattgt
    13141 tgagactttg gctattctgt acggtgatat tatggatata aaccctgaaa aattcaaaga
    13201 aagtgatcgg aattatatgg tcttatcaaa aggacatgct ggtccagcct tgtatagtac
    13261 actctattta aaaggttttt ttgataaaac attccttcat tcgctcaata caaacggtac
    13321 caaactacct tcgcatcctg accgcaattt aactcctggt atagatgtaa cgacaggctc
    13381 attaggtcaa ggcattagta ttgcaacggg aattgcttat gctcaaaaga ttgagaattc
    13441 aagctattat acttatacta ttgtaggtga tggtgaatta aatgaaggac aatgttggga
    13501 ggctatacaa tttgctgcgc atcatcaact gcaccattta attgtttttg tagatgataa
    13561 taaaaaacaa ttagatggtt tgacagctga tatttgtaat cctggagact ttgttgctaa
    13621 atttgaagct tttggatttg atgcagtacg tgtaaaagga gatgacattg aggcaattga
    13681 caaagctatt aaaacttttc aagattcaaa tagtgtcaaa ccaaaatgca ttgttttaga
    13741 tagtatcaag ggacaaggtg tgaaagagtt ggaggagtta gcttctaatc atcatttacg
    13801 accagattta caacaaaaaa caatgttaga gcgagcggtg ataagcttga gagaaagttt
    13861 ggaggtggta gaatgagatg ttcaaccaaa gaaatgaggc ttgtctatcg tgatttcctc
    13921 cttcaagcta atcaagaaaa taaacaaatt accgttttag aagccgattt gtcgagttca
    13981 atgtcaacaa atgtactggc atcagaattt ggaaaacgtt atattaatct aggtattatg
    14041 gaggctgaga tggtaggtct tgcagcaggg ctcgccatta aaggttacaa gccatacctt
    14101 catacttttg gtcctttcgc gtcaaggcgt gtctttgatc aagtattttt atcgttaggt
    14161 tactcacaat tatcagctac gattattggc tcagatgcag gagttagcgc agaaatgaat
    14221 ggcggaactc atatgccttt tgaagagtta ggtctactaa ggttaattcc aaaggcgact
    14281 atttttgagg ttagtgatga tattcagttt gaagcaattt taaaacaaac attaagtatt
    14341 gatggtctaa aatacattag aactattcgt aaggccccca ctgcagttta tgagggatgc
    14401 gaagattttt caaaaggttt tatacaatta cgccagggaa aagatattgc tcttgtagca
    14461 agtggtatta tggtttctag agcgatagaa gcagccgact acctcaaaga actagggatt
    14521 gaggcctcag ttatcgacct ttttaaaatt aagccccttc cagaagaact gaaaccatta
    14581 ctgatagatc aatccattgt aactattgag aatcataacc gtattggcgg tatcggaagt
    14641 gcattatgcg aatggcttag tatggagaaa gacactactg ttagtcgtat gggtattgat
    14701 gagcgatttg gtcaggtagg tcagatggaa tatttattag aggagtatgg actcgctgtt
    14761 aaagatatag tagagcattg caaatcaatt tataaatcat aacaaaaagg caacattgga
    14821 gttgcctttt tgttattttc tgaaattttt aagttttgta taagtatctt gtggtatttt
    14881 atccttacta aaaataaagg ggtggtcgtt atgcgagctt ggaaaggtat tgttttaatt
    14941 ttatctagca tagtggtgac actagtagca tggcaaaatg caggattaag tgaatttgtt
    15001 gtaccaggat tagctttgac aagtttatcg ctaacattcc tcttatcaac caaatttagg
    15061 atacttgaaa gttactttca aggtattgaa aatatgtatt tttaccacaa ggtaatggct
    15121 gtattttcaa tgattttgct tctattgcac aaaattggtc ttgtacaagg cggacatgga
    15181 agtgagtttg caaagactat tggcagtgcg ggattatatc tttttctaag tattgttttt
    15241 gtagcttatt tcggaaattt tttaaagtat gaaatttggc gttttattca tcgctttgtt
    15301 tatttagtct atattttggg attggtacat acttttctga ttttaggaga taggatacta
    15361 gggaatactt tactcagttt aatcgtatta ggttatgctg tcattggggt tatatctgga
    15421 ttttatatta ttttcctata ttctagaatg cgttttcgtc gtgtagggta cgttcaaaag
    15481 gttactcatc ttaaccatga tacaactgag attgaaatag caatgaagcg tccttatcga
    15541 tatgactatg gacaatttac ttttttaaaa atttatcaag ccggctttga atcagcagct
    15601 catcctttct cgatatcggg tggtcatgat agagtgatat ttttaactgt taaagcttca
    15661 ggagattata cgaaatccat ttacaaacag ctaaaagttg ggacaaaaat agccttagat
    15721 agagcttatg gtcatatgtt atttgataaa gacaagaaag aacaggtttg gattgcagga
    15781 ggtatcggca tcactccctt catttcattt attagagaaa atagtatttt gactaagaga
    15841 gttgacttct tttatacatt ttcaaatcaa gacaatttga tttatcaaga tatgttagaa
    15901 tcctatgcta aagccaatcc taattttaaa cttcacttaa ataatagtag cctccaagga
    15961 agacttgatt ttagtcagtc tgtttttgaa ggacaaccaa cgatatttat gtgtgggcca
    16021 actagtatga catctactta tgctaaggtc tttcggcaaa aagatgcaaa gtctcgttta
    16081 gtctatgaag gattttcctt tagagacagt tggttatcca tatttctatt aaaaactttt
    16141 gacaaagttt attctaattt gataaaataa gagggtctat aagacaaacc aactttttct
    16201 tggttttagg agtgccaatc ctaagactcg gatgaagcaa taaaaggaga atatattatg
    16261 gcaatctcaa aagagaaaaa aaatgaaatc attgctcaat atgcacgtca cgaaggtgat
    16321 acaggatcag ttgaagttca agtagcagtt cttacttggg aaatcaacca ccttaatgac
    16381 cacatcaaac aacacaaaaa agaccacgct acttaccgtg gattgatgaa aaaaattggt
    16441 caccgtcgta acttattagc atacctacgt cgtacagatg ttaaccgtta ccgtgaattg
    16501 attcaatcac ttggacttcg tcgttaatag acaacagagt caagtaaaaa cgctagaaaa
    16561 ttttctagtg ttttttgttt taaacgtact atgaacgtaa gtactttgag ggggccaaca
    16621 gtaagaaagt gttttaacct atgaagaggg ttattaagca agctaagatt aaccctagaa
    16681 atttatttta atcattgtaa caaggtctga ttgctagtat taataatcag actttttacg
    16741 tttacattaa tgaatgataa tacagcttcc cccacggatt ttcgctactc aattttatag
    16801 aattatgtta gaataaagct tatacgtggt tttatgcaaa gttgagttta gaacagctag
    16861 attgaacttt gtaggaaacg cgtaaacaaa aaatatggag aataatatgt caaaacaagt
    16921 ctttgaaatg atttttgctg gtaaaaaatt agttgttgaa actggccaag tggctaaaca
    16981 agcaaatgga tcagttgtcg ttcgttatgg agattctact gtacttacag cagcagttat
    17041 gtctaagaaa atgtcaacag gtgatttttt cccacttcaa gtcaattacg aagaaaaaat
    17101 gtatgctgct gggaaatttc cgggtggttt taacaaacgt gaagggcgtc caagtacgga
    17161 tgctacctta acagcacgtc ttattgatag accaatccgt ccgatgtttg ctgagggatt
    17221 tcgtaacgaa gtccaggtca tcaatactgt tctttcattt gatgaaaatg ctagcgcacc
    17281 aatggctgct atgtttggtt catcattagc attatcgatt tccgatattc ctttcaatgg
    17341 gccaattgct ggagttcaag ttgcttatgt tgatggcaat tttattatta atccgacagc
    17401 acaagaacaa gaggcttctg cgttagaatt aacggttgca ggtactaaag aagcgattaa
    17461 catggttgaa tctggagcga aagaactttc agaagaaatc atgctagagg ctcttcttaa
    17521 aggacatgag gctgtctgtg aattaatcgc ctttcaagaa gagattgtaa ctgctattgg
    17581 taaagaaaaa gccgaagtag aacttcttca agttgatcca gaacttcaag cagaaattat
    17641 tgctactcat aacatagctt tacaggctgc agtacaagtt gaagagaaaa aggctagaga
    17701 agcagctaca gaagcagtta aagaagtagt tataggtgaa tatgaggcgc gttacgctga
    17761 acatgaagaa tatgatcgca ttatgcgtga tgttgctgaa attttagaac aaatggaaca
    17821 tgcagaagtt cgtcgcctca ttacagaaga taaggttcgt cctgatggac gtcgtgtgga
    17881 tgagattcgt ccattagatg cagtgattga ttttttacca caagttcatg gatcaggtct
    17941 ctttacacga ggacaaaccc aagcattatc agtattaacg ttagcaccta tgggtgaagc
    18001 acaaatcatt gatgggctaa caccagagta taagaaacgc tttatgcacc actataactt
    18061 cccacaatat tcagttggtg agacgggacg ttatggagct gcagggcgtc gtgaaattgg
    18121 tcacggtgct ttaggagagc gtgcattaga acaagtcctt ccaagcttgg aggagtttcc
    18181 gtatgctatc cgactagttg cagaagtttt agaatcaaac ggttcatcat ctcaggcttc
    18241 tatatgtgct ggaacgcttg ctcttatggc aggtggtgtg cctataaaag ctccggttgc
    18301 tggtattgcc atgggactca tatcagatgg aacaaactac acagtcctta ctgatattca
    18361 aggattagaa gaccattttg gcgatatgga ctttaaggtt gcgggtacac gtgaagggat
    18421 tacagcactt caaatggata ttaaaattga aggtattacc cctcaaattt tggaagaagc
    18481 tcttgcgcaa gctaaaaaag cacgctttga aattcttgat gtccttcatg gagctatcgc
    18541 agagtcacgt ccacaattag caccaacggc acctaagatt gatatgataa aaatcgatgt
    18601 tgataagatt aaagttgtca tcggtaaagg cggcgaaact atcgataaaa tcatcgctga
    18661 aacaggtgtt aagattgata ttgatgaaga aggaaatgta tctatcttct ctagtgatca
    18721 ggctgctatt gatcgtacga aagacattat tgcaagttta gtacgtgaag caaaagtggg
    18781 agaagtttac catgctaaag tagttcgtat cgagaaattc ggtgcctttg tcaacctctt
    18841 tgataaaaca gatgcattgg tacatatttc agaaattgca tggacacgta cagcaaatgt
    18901 agctgatgtt cttgaaattg gtgaagaagt tgatgtgaaa gtcatcaaaa ttgatgataa
    18961 agggcgagtt gatgcctcta tgaaagcatt attaccacgt cctccaaaag ctgacaatcc
    19021 taaaaaggaa tcttaatatg acaagtacaa acgagttaga cattagattg cgtgctttta
    19081 tcaatgcacc ggataatttt ttagatagta taggtcttgt gaatgccttg caccactcta
    19141 cggtttgggc aagcaaggaa ccctatgcta ttcaagtaga tggtcaagaa gttgttcctg
    19201 tttttacgga tattactgac cttaatcatt tcaaagagga gcaagaaagt gctcgagata
    19261 tgttttggga gagtcgtcgt agtttagacg ttcttgatga agctatttca catggcttag
    19321 caggattggt ctataattta aaaaaagaag gcgattttgg gaactcgact attttctact
    19381 gtgaagatat ggtgcaattt atgaacaatt acaccacaat cttgaatcag ttgttaaatg
    19441 aagataacat tgttgccgat atcatggata aaacctactt ggtacctgcc tttgtccatc
    19501 cacgtgaaga aggtagtttt gaccgttttt tccctacaat gtctaccccc gaaggaaaaa
    19561 gttatgttcc agtattctca aaccttttaa gttttgaaaa atggtataac cataatgatt
    19621 ttggtggtgc ttttaggaaa gcacaaggag taattttagc ctggacgatt gacgatattt
    19681 ataaaccgcg taatggagaa aatgagattg atgatacctt tggagtagcc atcaatccat
    19741 ttgatgaaca acaagtttta gttgattggt ctgatgtaga aaacgattga ggaaaactat
    19801 ggggtggtgg aaagaaagta tagctattgt caaagaacaa gatccagctg ctcgtagttc
    19861 tctagaagtt attttaactt atccaggaat aaaagctctt gcagctcata ggctatctca
    19921 ttttttatgg aatcataatt ttaaattgtt agcacgtatg cacagtcaat tttggcgttt
    19981 ctggacacaa attgaaattc atcccggagc gacgatttcg gaaggggttt ttattgacca
    20041 cggttcagga ttggtcattg gagaaactgc cattgtagag aaaggtgcta tgctatacca
    20101 tggggttaca ttgggtggca caggtaaaga caaaggcaaa cgacacccaa ccatacgtaa
    20161 aggtgcgcta atttcagcac attctcaaat catcggtcca attgaggttg gagaaaatgc
    20221 taaagtcggt gctgcagcag ttgtccttgc agatgtgcca gcagacgtaa cagtagttgg
    20281 tgttccagct aaagttgtcc gtgtccatgg tcaaaaggat gatcttcaaa tccgaagtat
    20341 tgaacatgat cgagaagaaa gttattattc gtccaaactt taggaggtat ttgtgaagga
    20401 ttatgtaaca actcaaatat tatcaatttt aggtatacta gcaggagctt gtttaagcta
    20461 tttaaagttt gcacaaggta actctctctt aggaattatt tgggctgcct ttgctttaat
    20521 gtatattgtt cgtttgtata aagcttatca aaaacagaaa aaggaaaaac catgataaaa
    20581 atttacgaca ctatgactcg cagtttacaa gattttatac ctctcaatga aggtaaagtc
    20641 aacatgtacg tctgtgggcc aacagtttat aactatatac acatcgggaa tgcccgcagt
    20701 gtagtagctt ttgatactat tcgtcgttat tttgagtatt gtggttacca agtcaattat
    20761 atttctaact ttactgatgt tgatgataaa attataaaag gtgcagctga agcaggtatg
    20821 gatacaaaat ccttttcaga taagtttatt tcagcattta tggaagatgt tgctgcatta
    20881 ggtgtcaaac ctgctacaaa aaatcctcga gttattgact atatggatga gattattgat
    20941 tttgttaaag ttttagttga taaggaattt gcctatgaag ctaatggaga tgtttatttt
    21001 agagtaagta aatcacatca ttatgcaaaa ttagccaata aaactttgga agatttagag
    21061 attggtgcaa gtggtcgtgt tgatggggaa ggagagatta aagagaatcc tttagatttt
    21121 gcactttgga aatcagctaa atctggggaa gtctcatggg aaagtccttg ggggaaaggg
    21181 cgcccagggt ggcatattga atgttctgtt atggcaactg agatccttgg tgatactatt
    21241 gatatccatg gtggaggagc tgatttagaa tttccacatc acacgaatga gattgcacaa
    21301 tctgaagcta aaacaggtaa gacctttgca aattactgga tgcataatgg ttttgttaac
    21361 gttgataacg agaaaatgtc taaatctctt ggtaacttta ttactgttca cgatatgttg
    21421 aagagcgttg acggacaagt aatacgtttt ttcttagcga cacaacagta tcgtaaaccc
    21481 gtgaatttca ctgaaaaggc cgttcatgat gcggaagtta accttaaata tttaaaaaat
    21541 actttcaact taccaatcca agagaatgct aatgatgaag aattagaaca atttgtgaaa
    21601 gcatttcaag aagcgatgga tgatgatttt aatacagcaa acggtattac tgttatcttt
    21661 gaaatggcaa aatggattaa ttcaggacac tatacttcaa aagttaagga aacccttgct
    21721 gaattattag aaatttttgg tattgtattc caagaggaag ttcttgatgc tgatattgaa
    21781 tctttaatag aacaacgtca agaagcacga gctaatcgtg attttgcaac tgctgatcgt
    21841 attagagatg aattagcaaa acaaggaatt aaattgttag atactaagga tggcgtgagg
    21901 tggactcgtg attgatgttc gtttgattaa tggtattgct ttagcttttg aaggagatgc
    21961 agtttattct ttgtatatcc gacgccattt aattatgcag ggttttacca aacctaatca
    22021 gttacatcgg aaagcaaccc aatatgtttc tgctaatgcc caagcattgt taattaatgc
    22081 catgttagaa gaaaatattt taactgatga agagcagtta atttataaac gaggacgtaa
    22141 tgcgaatagt catacaaaag ctaaaaatgc tgatattatt acctatcgta tgtctacagg
    22201 ttttgaggct cttatggggt acttggatat gactggtcag ataaagcgct tggaaactct
    22261 aatacagtgg tgtattgaaa ccattgaaaa ataaacaaaa agagaggcta tatcctctct
    22321 tttttataga gacattagga tttgttggta aaatattgct ataaaaagct ttatgctata
    22381 gcattcatga tataatatga ctatgaaaga taaacaattt aaagaagaat caagtgacct
    22441 tgtctatggc ttacatgctg tgacagagag tttaagagct aatactggta ataaattata
    22501 cttgcaagat gacttaagag gaaagaacgt tgataaagtc aaagcactag cgactgaaaa
    22561 aaaagtatcc atttcctgga ctcctaaaaa aatcttatca gacatgacaa atggtggagt
    22621 tcaccaagga ttcgttctaa aagtttcgga atttgcctat gcggatttat cagagataat
    22681 gacaaaggca gagaatgaag aaaatcctct aattttaatc cttgatggtc tgaccgaccc
    22741 tcataaccta ggttctattc tacgcacagc tgatgcaacc aatgtaacag gcattatcat
    22801 ccccaaacat cgttctgttg gagtgacccc ggtggtttca aagacatcta caggagctgt
    22861 tgaacatgtt cctatcgcaa gggtaactaa tctcagtcaa actttggata ctttaaaaga
    22921 taaggacttc tggatatttg gcactgatat gaatgggaca ccatcgcata agtggaatac
    22981 caaaggtaaa ttagccctag ttattggtaa tgaaggaaaa ggtatttctc ataatatcaa
    23041 aaagcaagta gatgaaatga ttacaattcc catgaatggt catgttcaga gtttaaatgc
    23101 cagtgtcgca gctgctattt tgatgtatga agtatttaga aaccgcctat gaaaaaacat
    23161 tctattttac tcgttgatgg ctacaatatg attgcttttt ggaaagatac tcgacagctt
    23221 tttaaaagca atcgtttaga agaggcaagg gaagtattac ttagaaagct aaatcattac
    23281 gcacatttcg aacacattga tattatttgt gtatttgatg cccagtatgt tcctggtgta
    23341 cgtcaacgtt atgaccaata caaaatatcc gttatcttta ctgaggagga cgaaactgcg
    23401 gatagctata ttgaacgagc agcggcagaa cttaatcaat ctgtgttaaa tttagtctcc
    23461 gttgctacca gtgatttaaa tgaacaatgg actatatttt ctcagggtgc tttacgtgtc
    23521 tcagctagag aattggaaca gcgtgtagcg acagtaaaat cagatttgga taagatgtcc
    23581 agtcaaatcg atttgagtac gccaaagtta aggccgtgga atgatgaaca actaggaaaa
    23641 ctcaaggatt ttttagatgg tatgtaaaaa ctaacacttt attagccagc caacaatttt
    23701 tttgatataa tattggaact actttgatta tcaaataaaa ataatagatt gaggtaacaa
    23761 tgacttttaa aatactaacg gattcgacat cggatttaga tgaaaagtgg gctcaagagc
    23821 acaatgtcga tattataggt ttaaccattg aacttgatgg aaagacttat gagacagtcg
    23881 gagacgaaaa aattactagt gacttcttgt tggagcgtat gcaagaggga gcaaagccta
    23941 caacaagcca aattaacgtt ggtcagtttg aagaagtttt ttcaacctat gcagaaaatg
    24001 accatgcctt gttatattta gcgctatctt cacatttatc agggacttac cagagtgcaa
    24061 ctattgcgcg tgagatggtc ttagacaaat atcctgatgc tcaaattgag attgtggata
    24121 caatggcagc ttcatgtggt gagggtgtct tagcgatgtt agcaactaaa gagcgacaag
    24181 aaggtaagtc tttagaagaa gtgaaacaaa aaattgaatc acttctccct aaattaaaca
    24241 catacttttt agttgacgat ctcaaccatt taatgcgtag tggtcgctta tcaaaaggtg
    24301 cagctatcat tggaagtgtt gctaagatta aaccattact taagttagat agtgagggta
    24361 aattagtacc atttgccaag acaaggggcc gtaaaaaagg tattaaagag attgtgactc
    24421 aggcaacaaa aacactatct tatagcactt taattattgc ttatagtgga gagaaagatt
    24481 cagctcaagt tatgaaagaa cagttattag cagatgagcg tattgaagaa gttattattc
    24541 gtccattagg gccagttatt tcagctcatg tgggctcagg tgctttagct ctattctctt
    24601 taggggaaga aaaccgataa caaatcaaag ggcataacag tacgttatgt tcttttagta
    24661 tacattttat aaaatgtatt ttatatgatg ctatgaaact ataaaatagt aggagacata
    24721 ttatgaaaaa taaaataaga actatcctca atatgatgaa cgacaacaac aattggtttt
    24781 acaaagttat caaaaatgtt tttagacgat gttttttatc ttatggcttg aaagtgtttt
    24841 tggaaagcta ataaagggaa gcgtgtcacc tgattttata ataatattaa ctttatttag
    24901 tggtatttct attttgttac gacttctaat ctaaaaagag tctctccgtt tgttgatgaa
    24961 agattttgga aaatgttttt gttgctggcg catttgccct cataggaata actattttat
    25021 tgatgctttt actaggaagt tgatattgct acttattttt caaggggaaa tgatgcaatt
    25081 ttagcagtta taggaatatc attgatagct atgggaactt cagtatacta ccttaagtat
    25141 aaggacagat agtaatatga aaaatttacg cttaaagaca gctagagcta gtatggattt
    25201 attacaacag tctctagctg aaaaggtcgg tgtatcatgt cagacaatag ccgccattga
    25261 aaaaggaggc tataattaag cgattaaatt gtgtcaagct ttgaataaaa ctgtagatga
    25321 tttattttgg gatgaaaatt gataaaataa aagaaataac ttgacgaatg ctaccttatt
    25381 ttgatatgat tataagcggt attgtttacc ccatttgtaa ggccccggaa cctttcaaat
    25441 actctcgtgg accggaacat ccacacctgt aaacaaaaac gaattcgtat aggagaaatc
    25501 atgaacaaaa caactttcat ggctaaacca ggccaagttg aacgcaaatg gtacgttgtt
    25561 gacgcaacag atgtacctct tggacgtctt tctgcagtag ttgctagcgt acttcgcgga
    25621 aaaaacaaac caactttcac accacacact gatacaggtg actttgtgat tgttatcaat
    25681 gctgaaaaag ttaaattaac tggtaaaaaa gcatcagata aaatctacta cactcactca
    25741 atgtatccag gtggtttgaa acaaatctca gcaggtgaac ttcgttctaa aaatgctgtt
    25801 cgtttgattg aaaaatcagt taaaggcatg cttccacata acactcttgg acgtgcacaa
    25861 ggtatgaaat tgaaagtatt tgttggcggt gagcatacac atgctgcaca acaaccagaa
    25921 gtacttgata tctcaggact tatctaagag gaaaggagca ttatataatg gcacaagcac
    25981 aatatgcagg tactggtcgc cgtaaaaacg ctgttgcacg cgttcgtttg gttccaggta
    26041 ctggtaaaat tacaatcaat aaaaaagatg tagaagagta catcccacac gcagaccttc
    26101 gtcttgttat caaccaacct ttcgcagtta cttcaactca aggttcatac gatgtttttg
    26161 ttaacgttgt aggtggtgga tatgcaggtc aatcaggtgc gatccgtcat ggtatctcac
    26221 gtgcactttt agaagttgac ccagatttcc gcgattcatt gaaacgtgct ggacttctta
    26281 cacgtgacgc acgtatggtt gaacgtaaaa aaccaggtct taaaaaagca cgtaaagcat
    26341 cacaattttc aaaacgttaa tatactgtta tatcaacgtt tcaaagcact ttgcaagcaa
    26401 ttgcaaggtg tttttttatt gtcaatttta cttttgataa cctgataacc ccttcgcgaa
    26461 tatattgcaa tacatagtac actttttgga tgtaaattga tgagtattag ctagtttata
    26521 ctatataaca ccatatatta cagttgtcag agaaaaccag gtcttaaaaa agcccgtaaa
    26581 gcatcacaat tctcaaaatg ttaatatact gttatatcaa cgtttcaaag cactttgcaa
    26641 gatattgtag ggtgtttttt taaaatcttt tgataacctt tccgttttga aagtaaagta
    26701 gatgtaatat aaatcgctgt ttgtattttt aatagattaa cctattatga gttttatctg
    26761 tgtaaggaac atctaaatat gctatactgc agttaaaaaa atagggagca aatgatgtca
    26821 caaattaaac taacacctga agaactccgt atttcagcac aaaaatatac aacaggatca
    26881 caatcaatta cagatgtgtt aacagttttg actcaagaac aagctgttat tgatgaaaat
    26941 tgggatggta cagcatttga tagctttgaa gcccaattca atgaattatc tccaaaaatc
    27001 acacaatttg cacaattatt agaagatata aatcaacaat tattgaaagt tgcggatgtt
    27061 gtcgaacaaa cagactcaga tattgcctca caaattaata aataattggc ttattcaaat
    27121 tcataagttg atatattata gctagatgat gtaattgttt cataaaggag taaaaatgaa
    27181 aaagagtacc caaataatac tactaatagt tgcattattc atacttgttt ttagcggagg
    27241 attttatatg aaagaacaac aaagaaaaga agaactaaaa cggaatcgag aatatgaagt
    27301 tagtctagtc aaagcattga aaaatt
//
LOCUS       DATRNX010000014        26658 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRNX010000014 DATRNX010000000
VERSION     DATRNX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26658
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1478
                     /locus_tag="VBC81_000488"
     CDS             <1..1478
                     /locus_tag="VBC81_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6911550.1"
                     /translation="MFAMNNASTNIYLFTFMFVTYFSTGVLGSAAIFVSQIMGYIRIF
                     DGFIDPAIGIMIDKTDTKFGKYRPILIIGNVITALSLIFLLALRGVDENIRFPLFILV
                     LIIHKIGYSMQQTITKAGQTALTNDPKQRPIFNIVDAVMTTSLMTGGQFVVSVFLVPK
                     FGNFTPQFFNVLIFGTILISAILAIVAIIGIWAKDRKEFFGLGENTQKTALKDYWKVL
                     KGNKPLQILSIAAALVKFAIQFFGDSVVMVLLFGILFGNYALSGQFSLLFIVPGVIIN
                     ILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAVLYVGKPGDLSLTSLNLYTILFIVTN
                     IIARYASQAPASLVLTMGADISDYETSESGRYVSGMIGTIFSLTDSIASSFAPMVVGF
                     VLAGIGFSKSFPTIETPLPPDLKMAAISILVAIPFIALSIALLLMKFYKLDKEEMVRI
                     QEKIQVMKAATTKERAKDIARNVPLTDMDYVDVTKYPIDKD"
     gene            1545..2570
                     /locus_tag="VBC81_000489"
     CDS             1545..2570
                     /locus_tag="VBC81_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6911551.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            2587..4386
                     /gene="uidA"
                     /locus_tag="VBC81_000490"
     CDS             2587..4386
                     /gene="uidA"
                     /locus_tag="VBC81_000490"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6911552.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            4415..5086
                     /locus_tag="VBC81_000491"
     CDS             4415..5086
                     /locus_tag="VBC81_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6911553.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            5203..5820
                     /gene="eda"
                     /locus_tag="VBC81_000492"
     CDS             5203..5820
                     /gene="eda"
                     /locus_tag="VBC81_000492"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6911554.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            5837..7237
                     /gene="uxaC"
                     /locus_tag="VBC81_000493"
     CDS             5837..7237
                     /gene="uxaC"
                     /locus_tag="VBC81_000493"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6911555.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            7255..8301
                     /gene="uxuA"
                     /locus_tag="VBC81_000494"
     CDS             7255..8301
                     /gene="uxuA"
                     /locus_tag="VBC81_000494"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6911556.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            8425..9264
                     /locus_tag="VBC81_000495"
     CDS             8425..9264
                     /locus_tag="VBC81_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6911557.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            9417..10229
                     /locus_tag="VBC81_000496"
     CDS             9417..10229
                     /locus_tag="VBC81_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6911558.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            10245..12035
                     /locus_tag="VBC81_000497"
     CDS             10245..12035
                     /locus_tag="VBC81_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6911559.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(12093..13178)
                     /locus_tag="VBC81_000498"
     CDS             complement(12093..13178)
                     /locus_tag="VBC81_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6911560.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            13388..14392
                     /gene="ccpA"
                     /locus_tag="VBC81_000499"
     CDS             13388..14392
                     /gene="ccpA"
                     /locus_tag="VBC81_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6911561.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            14524..15990
                     /locus_tag="VBC81_000500"
     CDS             14524..15990
                     /locus_tag="VBC81_000500"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6911562.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            16035..17033
                     /locus_tag="VBC81_000501"
     CDS             16035..17033
                     /locus_tag="VBC81_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6911563.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            17035..18369
                     /locus_tag="VBC81_000502"
     CDS             17035..18369
                     /locus_tag="VBC81_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6911564.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            18826..20769
                     /gene="thrS"
                     /locus_tag="VBC81_000503"
     CDS             18826..20769
                     /gene="thrS"
                     /locus_tag="VBC81_000503"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6911565.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            20990..21694
                     /locus_tag="VBC81_000504"
     CDS             20990..21694
                     /locus_tag="VBC81_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911566.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            21792..22811
                     /locus_tag="VBC81_000505"
     CDS             21792..22811
                     /locus_tag="VBC81_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6911567.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            22978..23544
                     /locus_tag="VBC81_000506"
     CDS             22978..23544
                     /locus_tag="VBC81_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6911568.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(23593..24243)
                     /locus_tag="VBC81_000507"
     CDS             complement(23593..24243)
                     /locus_tag="VBC81_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6911569.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(24255..24950)
                     /locus_tag="VBC81_000508"
     CDS             complement(24255..24950)
                     /locus_tag="VBC81_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6911570.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(24963..25763)
                     /locus_tag="VBC81_000509"
     CDS             complement(24963..25763)
                     /locus_tag="VBC81_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6911571.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(25775..26530)
                     /locus_tag="VBC81_000510"
     CDS             complement(25775..26530)
                     /locus_tag="VBC81_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6911572.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
BASE COUNT         8684 a         4105 c         5436 g         8433 t
ORIGIN      
        1 ttatgtttgc catgaacaat gcttctacaa atatatactt atttacgttt atgtttgtta
       61 cctatttctc aactggcgtt cttggctcgg cagccatttt tgtcagtcaa attatgggtt
      121 atatccgtat ttttgatggt tttattgatc cagctattgg tattatgatt gataaaactg
      181 atacaaaatt tggtaagtat cgtcctattt tgattattgg gaacgttatt acggctttgt
      241 cattgatttt tcttcttgca ttgagaggtg tcgatgaaaa tattcgtttc ccattattta
      301 tccttgttct aattatccat aaaattggat actcaatgca acagacaata actaaagcag
      361 ggcaaacagc actgacaaat gaccctaagc aacgtcctat ttttaatatc gttgatgcag
      421 ttatgacaac atcattaatg acaggcggtc agtttgtcgt ttcagttttc ttagtaccaa
      481 aatttggtaa ttttacacct caattcttca atgtccttat ttttggaaca attcttattt
      541 cagctatttt agcaatcgtt gcaattattg ggatttgggc aaaagacaga aaagaattct
      601 ttggtttagg agagaatacc caaaagactg ctctaaaaga ttactggaag gtgttgaaag
      661 gaaataaacc gcttcagata ttatccattg ctgcagcttt ggtaaaattt gccatccaat
      721 tctttggcga ttccgttgtt atggtgctat tgtttggtat tttatttggt aattatgctt
      781 tatcaggaca attctcactt cttttcattg ttcctggtgt tattattaat attttgttct
      841 caacaattgc tcgtaaaaaa ggtcttcgtt tttcatatgt cagagcaatt cagatcggta
      901 tgattggttt acttgctttt ggtgcagttc tttacgttgg taagcctggt gaccttagtt
      961 taacaagcct aaatctttat actattttat ttatcgttac taatattatt gcgagatacg
     1021 cttcacaagc acctgctagt ttagtattga ctatgggagc agatatttct gactatgaaa
     1081 cttctgaatc cggtcgttat gtatcaggaa tgattggaac tatcttctcc ttaacagatt
     1141 cgattgcttc ttcatttgct cctatggtag taggttttgt gttagcagga attggatttt
     1201 caaaatcatt tccaactatc gaaacaccac tgccccctga tttaaaaatg gcagctatta
     1261 gtatccttgt ggccattcca tttatagctt tgtctatagc tcttctttta atgaaatttt
     1321 acaaactaga taaagaagaa atggttcgta ttcaagagaa gattcaagtt atgaaagcag
     1381 caactactaa ggaacgtgct aaagatattg ctcgtaatgt tcctttgaca gatatggatt
     1441 atgttgatgt aacaaaatat cctattgata aagattaaaa agtttagctg ctcttcaata
     1501 gagcagctat tttgttaaat aagagctacc attggaggat tcttatggca aaaattattt
     1561 cattaggtga ggttcttttg cgattatctc cacctcaata tcacacactg atgcaggcta
     1621 atcacctaaa atgccaattc ggtggttcag aacttaatgt cttagcaagc cttgctcaac
     1681 taggttacca tgtaggattg gtctcagctt tgcctgataa tgatttgggc aagatggcaa
     1741 gtcaatttat attgtcacag caaattagtc cagctgctat aattaaaaaa gaaggacgac
     1801 taggaattta ttactatgaa caaggatttt cggtaagaac aaataaggtt atctacgatc
     1861 gtaactattc atctttttgg gaaagtacct tgagtgatta tgattttact tctattttta
     1921 agggagtgga ttggtttcat gtttctggaa ttactccagc actaaccaag gacttatatg
     1981 aggtgactcg gttccttatg acaaaagcta aagaaggagg tgtaaaagta tcgattgatc
     2041 tcaattttcg tgagagttta tggtcatctt ttcaagaggc acgtgagcaa ttatcgccac
     2101 tattaggatt attagatgtt tgctttggct tagagccgat ttatttagct ggcgaaagtg
     2161 aagatttaaa agatgagttg ggcttatcca gaccttacct tgatattgaa ttacttgaaa
     2221 agataaccca aaagattgtc caagaatatg ggttagacta tattgccttt actcaaaggg
     2281 aaatggaata tactaatcag tatatgttaa aatcatattt gtatcataat aatatgttat
     2341 atcaaactga taaaactggc gttgaagtat tagatcgtgt ggggacagga gatgcctttg
     2401 ctgcaggttt aattcatgcg cttctggaaa aagaaactcc tcaaagggcc ttagaaatag
     2461 ctatggctac atttaaatac aaacatacca ttcaaggaga tatcaatatc atgacacgag
     2521 atgatattgc ttacctcatt gaaaaagaaa caaatgatat taaacgatag tgtaaaagga
     2581 gagtgtatgt tatatccatt attgacaaaa acaagaaata cttatgattt aggcggtatt
     2641 tggaacttta aattaggaga acataatcca aatgaattac taccttcaga tgaagtcatg
     2701 gttatcccga cttcatttaa tgatttgatg gtaagtaaag aaaaacgtga ttatataggt
     2761 gatttttggt atgaaaaagt cattgaagta cctaaagtat cagaggatga agagatggtg
     2821 ctgcgttttg gctcagtgac acatcaagct aaaatttatg ttgacggtgt attggtagga
     2881 gagcataagg gaggttttac tccttttgaa gttcttgttc ctgaatgtaa gtataataat
     2941 gagaaaatta aggtatcaat ttgtgctaat aacgttttag actataccac gcttccggtc
     3001 ggaaattata gtgaaatcat tcaagaagat ggtagcatta agaagaaagt acgtgagaat
     3061 tttgatttct tcaattatgc aggtgtgcat cgtcctctta agttgatgat acgtcctaaa
     3121 aatcatattt ttgatattac aattacaagt cgtctatcag atgatttaca gtcagcagat
     3181 ttacattttt tagtggagac taatcaaaag gttgacgaag ttagaattag cgtttttgat
     3241 gaagataaca aactagtggg tgaaacgaag gatagcagat tatttcttag tgatgtccat
     3301 ctttgggaag ttttaaatgc ctatctgtac acagcgcgtg ttgaaatttt tgttgataat
     3361 caactccaag atgtctatga ggaaaatttt ggtcttagag aaatagaagt gacaaatggt
     3421 caattcctat tgaatcgcaa acctatttat tttaaaggat ttggtaaaca tgaagatacg
     3481 ttcattaatg gcagaggttt gaatgaagct gctaacttaa tggatttgaa tcttttgaag
     3541 gatatggggg caaactcttt tagaacatcc cattatcctt attctgaaga aatgatgcgt
     3601 ttagcagatc gaatgggagt gttagtgata gatgaggttc cagcagtagg gttatttcaa
     3661 aattttaacg cttccttaga tttgtcacca aaagataatg gtacgtggaa tttgatgcaa
     3721 acaaaagcgg cgcatgaaca agctatccaa gaattagtga agcgtgataa aaatcatcct
     3781 agcgtcgtga tgtgggtagt cgctaacgaa ccggctagtc atgaagcggg agcacatgat
     3841 tattttgagc cattagtaaa actttataaa gatttagacc ctcaaaaacg tcctgtcacc
     3901 ttggttaata ttttaatggc aaccccagat agagaccaag tgatggacct ggttgatgtt
     3961 gtctgcctta atcgttacta tggttggtac gttgaccacg gtgatttaac aaatgcagaa
     4021 gtaggtataa gaaaagagtt attagaatgg caagataaat ttcctgacaa accaattatc
     4081 ataacggagt atggcgctga tacgttaccg ggattacatt ctacttggaa tattccatat
     4141 acagaagaat ttcaatgtga tttttatgaa atgagtcatc gtgtttttga tggtattcct
     4201 aatttagttg gtgagcaagt ctggaatttt gcagactttg aaactaatct gatgatactt
     4261 cgtgtacagg ggaatcacaa aggtctcttt tcaaggaatc gccagccgaa acaagtcgtc
     4321 aaagaattta aaaaacgctg gatgactatt cctcattacc ataataaaaa aaatagtgta
     4381 aaataggggt agttatttta gaagaggtga tgatatggct agacccttag tagaacaaac
     4441 agcagatcgt ctattgcatt taattttaga aagagaatac cctgttggtg ctaagctccc
     4501 caatgaatat gaattagcag aagatctaga tgttggtcgc agcacaatcc gagaagcagt
     4561 cagaagttta gcaacgcgta atattttaga agtcagacag ggctctggaa catatattag
     4621 ttctaaaaag ggtgtttctg aggatccatt agggttctct ctaataaagg acacagatag
     4681 gttgacatca gatctctttg aactacgcct tttattggaa cctcgaatag cagagctggt
     4741 agcttataga attacagatg atcagttaca attactcgaa aagcttgttg gagatattga
     4801 agatgctgtt cacgcaggcg atccgaaaca tttactttta gacgtggaat ttcattccat
     4861 gctagctaaa tacagtggta atattgcaat ggatagcctt ttgccagtca tcaaccagtc
     4921 aattcatctg ataaatgcga attatacaaa tcgtcagatg aagtcggata gtttagaagc
     4981 acatagagaa attatcaaag caatccgaga aaaaaatcca gtagctgcac atgatgctat
     5041 gcttatgcat atcatgagtg ttagacgttc agcgttgaaa gcttaatata agaagaaaaa
     5101 tagctattct tgaatagcta tttttttagt attcttcttg acaattcatc agacgactta
     5161 tataatatag ttaaagataa cggttacatt ttgggaggta ttatgttaaa tcaattgaaa
     5221 aacaactatt tctttgctgt tattcgaggg aaatcatctg aggatgcgct cgaaatcgca
     5281 aagcatgcta ttttgggtgg tattcggaat attgaagtaa ctttttcgac tcctgaagca
     5341 agcaaagtca tcaaacaatt atcagatgac tttaaaaata ataaagaaat tattgtgggg
     5401 gccggaacgg tcatgacaac tgaacttgct aaagaggcta ttgatgctgg agctaaattt
     5461 ttggttagcc cacactttga ttcagatatt gctaatttag ctaatgaaaa caaggtttat
     5521 tatttcccag gctgtgcaac ggcaacagaa atcgtggtag ctagaaagta taaatgtcag
     5581 attataaagc tttttccagg tggagttgtg ggtccaggtt ttatcaaaga tattcatggt
     5641 cctattccag atgttgattt aatgccatca ggtggcgtct ctgtttcaaa tgtagttgaa
     5701 tggagaaaag caggagcagt tgcggttggt gttggctcag cattatctag taaagtagcc
     5761 acagaaggct atgatagtgt tactaaaata gctaaacagt ttgtttctgc acttgattag
     5821 tgagtaagag aagaatatgg cgtttaatac tgaaacattt atgttaaaaa atcaagcagc
     5881 tatacagctg tatgaagagg tcaagagaca acctattttt gattatcatt gccacttaga
     5941 tccaaaggac atttttgaag atcatatttt tgataatatt gtggatttgt ggcttggtgg
     6001 tgaccactat aaatggcgtc ttatgagggc taatggtatt tctgaagcag aaattacagg
     6061 tccagcatcc aaccttgaga aatttaaagc atttgctaga acactagaaa gagcatacgg
     6121 taatcctgtt tatcattggt ctgctatgga attgaaaaat atctttggtg ttaatgagat
     6181 tttgacagaa tcaaatgctg aggaaattta tcatcgttta aatcatttct tgaaagagca
     6241 taaaataagt cctagacgtt taattgcaga tagcaaagtt atgtttattg gtactacaga
     6301 ccaccctctt gatactctcg agtggcataa aaagttggct gctgatgaga gttttaaaac
     6361 agttgttgct ccgacattta ggccagatga agcttttatt gaacatcgac attttgttga
     6421 ttttattact aaactgggtg acattactca aaaagagatt acagattttt caacatttat
     6481 agcagcaatg gaagaacgca ttgcgtattt tgctcaaaat ggttgtaggg ctagcgatat
     6541 cagttttacg gaaatcgtct ttgaacaaac tgatgaattg gagttgaacg atttatttaa
     6601 taaagtttgt gaaggctata tcccaaatca atcagaaatc agcaaatggc aaacagctgt
     6661 ttttatggaa ctttgccgat tatataaaaa atatggtttt gtaacacagg tgcattttgg
     6721 agctttgaga aataatcatt ctactatttt tgaaaaattg ggagctgacg tgggtgtgga
     6781 ttcacttggt gaccaagtgg ctttaactgt taatatgaac cgtttattgg attccttggt
     6841 taaaaaagat agtctgccta aaatgatttg gtataatctc aatccagctt acaatatagc
     6901 agtagctaac actttggcaa attttcaagc taacgaactt ggagtgagat cttaccttca
     6961 atttggtgca ggttggtggt ttgcagatac taaattaggg atgattagtc aaatgaatgc
     7021 cttagcagag caaggcatgt tggcgaattt tattggtatg ttaacagatt ctagaagttt
     7081 tctatcttat caacgtcatg actattttcg acgtatttta tgtacctact taggagaatg
     7141 gattgaagaa ggagaagttc cagaagatta tcaagcttta ggaagtatgg ctaaagacat
     7201 cgcttatcaa aatgcagtta attattttaa gaactaaaaa ggaagagtga aatcatggaa
     7261 atgtcattta ggtggtatgg tgaagacgac cccgttactt tagaaaatat tggtcaaatt
     7321 ccaacaatga aagggattgt tacagcgata tacgatgtac cagtaggaga agtttggtca
     7381 cgtgagcgca ttcaacagct gaaagaaaag gtagaggcag cagggcttaa aatttcggtt
     7441 atcgaatcag tacctgttca tgaagatatc aaattgggac gtccaacacg tgatctcttg
     7501 attgataatt atattcaaac cgtcaaaaat ttggctgcgg aaggtattga tactatttgt
     7561 tataatttta tgccagtatt tgactggaca cgcactgatt tagcatatca atatccagat
     7621 ggttccacag ctttgatatt tgatgagacg gtttctaaaa aaatggatcc agtaaacggt
     7681 gaattatctt tgccaggatg ggatgccagc tatagtaaag aagaaatgaa ggctatcatg
     7741 gatgcctatg ctgaaattga tgaggaaaaa ctttgggaaa atctcactta ttttattaaa
     7801 cgtattattc ctgaagcgga agccgtaggc gttaaaatgg ccattcatcc ggatgaccca
     7861 ccatattcta tttttggttt acctcgcatt attactgggt tagaggctat tgagcgtttt
     7921 gtgaaattgt acgatagtaa aagtaatggc atcactttat gtgtgggttc ttacgcttct
     7981 gatccccaga atgatgtttt agagatttcg cgacgtgctt ttgagttaga tcgtgtaaac
     8041 tttgttcatg ctcgcaatat taaattaggt gatggaaagt catttaaaga atcggctcat
     8101 ccatcagaat acgggtcaat agacatgtat gaagttatta agttatgcca tgaatttggg
     8161 tttgagggtg ctattcgacc tgaccacggg cgtatgattt ggggagaaac aggtcgtcca
     8221 ggatatggcc tatacgatag agctttagga gccacttacg tttctggttt atacgaagct
     8281 gtgattaaag gttcaaaata aggcgatgct tccaaatttc tcctgttcca atctcctgaa
     8341 agagaaattt ggaaagttat tatcataata tgattgtact tagctaagag tagaatactt
     8401 atagaagtta atgagaggaa taagatgtca aaaacaatta catttaccaa taaagttgtc
     8461 gtcataacag gtgcaggcgg tgttctctgt ggttatatgg ctaaagaatt tgcgaaagca
     8521 ggcgctaaag tggcattatt agatttgaat caagaagctg ctcaaacatt tgctgatgaa
     8581 attgtagaag aaggtggtat cgctaaggct tataaagcta acgttttgtc taaagaaaac
     8641 cttgaagaag tgcatcaagc cgttttagag gatcttggac caactgatat tttagttaat
     8701 ggtgcaggag gcaacaatcc taaagctaca actgataatg aatttcacga attagactta
     8761 ccttcagaaa caaaaacctt ctttgagtta gatgaagctg gtattagctt tgtttttaac
     8821 ttaaattatt taggaacatt attgccaacg caagtatttg cccaagatat ggttggacgt
     8881 gaaggagcga atattattaa tatctcttca atgaatgctt tcacccctct gaccaaaata
     8941 ccagcttatt ctggagctaa agcagctatt agtaacttca cacaatggct agctgttcat
     9001 ttctcaaaag ttggtatccg ttgtaatgct attgcacctg gtttcttggt aacaaatcag
     9061 aaccgaagct tgttatttac tgaggacggt cagccaactg cgcgtgctga gaaaattctt
     9121 aataatacac caatgggaag atttggagaa gcaagtgagc tgattggcgg attgttcttt
     9181 ttagcagatg aaaaatcatc aagttttgtt aatggcgttg tccttccgat tgatggtgga
     9241 tttgctgctt actctggtgt ttaatttaca agataatagc cacttaacac ataagagatt
     9301 attgagttga gtggtttttt aaggaaattt gtttaatgat cttaccacta aatgatgggt
     9361 aggttgctaa ttggaaaatc ctcataagtt agataaattt agaaactgga gataatatgg
     9421 tagatacaaa tcacaaaatc gctttttgca tagattctga tggttgcgca atggatacaa
     9481 tgacatataa gcatcaactc ttttttggtc caattgctgc agatatattt gggatatcag
     9541 aacaagaaag aacaagtttt ttaaaagagt gggatagagt caatctttat tcaagaactc
     9601 gtggagttaa tcggtttgtg ggattggtta tgggccttga gtttgccgga gtagaacata
     9661 ttgataaatt aaaagaatgg gtcgaaacaa cagactcatt gtccaataat tccttagagt
     9721 tagcgcttgc caacgatttt agtagagatt tagagaaggc tttagaatgg tccaatgaag
     9781 tgaatcgcca aatcaaaaat tatgagggag attctttagc gttttcaggt gtgttagaaa
     9841 gtttagaacg attaagtcaa tttggtaatg tttatgtcgt gagttctgct aacaaggaag
     9901 ctgtttggca agagtgggaa gaacagggat tgctaaagta tgttgatgat atctattgtc
     9961 aagatagagg taaaaaagaa gatgttatcg ccactttaat tgcaagtggt tactgccgtg
    10021 aaactatttt gatggtagga gattccctgg gtgatctggc tgcagcagaa caaaatggta
    10081 ttgcatttta cccagtactt gttggtaagg aagtcaaatc atgggaaatt cttagagaag
    10141 atattggtga agcttttgca aaaggtcaat tcgaacagca aaggcaaaaa gaatctatta
    10201 ataccttttg ggctaattta gataactaat agaaaggata ggctatgaca catttagttg
    10261 acttaacaaa gaaacccttc aatctaaatc aagaagcaat tgagtggatt gaaaaaacaa
    10321 ttaatgagat gacattggat gagaaaattg ggcaactttt tttcaatatg ggagctagtc
    10381 gttcagaaga atatttaaca gatgttttgg atcggtatca tattgctgct gttcgctata
    10441 atagaggtag ttcaagtgaa atctatgatc agaatttaat tttacagaca aagtcgaaat
    10501 tgccgatgtt gattgctgca aatacagaag ctggtggaga cggtgctgtc acggatggaa
    10561 ctaaagttgg cgatgaaata aaagtggctg ctacaaatga tccgaaatat gcttatgaaa
    10621 tgggaagaat cgcagggatg gaggcatctg ctgtgggttg taatgcttca ttttctccaa
    10681 ttgtagattt gacacgtaat tggcgtaatc ctatcatagc tagtcgaaat tggggtgcta
    10741 acgttgatca aattatttca ctttcaaaag agtatatgaa aggaatcatg caatataata
    10801 ttgtaccttt cgctaaacat tttccagggg atggcattga tgagcgtgat catcatttgt
    10861 catttgcttc caatcctatg agtaaagagg agtggatgtc gacatttggc cgcatttatg
    10921 gtgaattggc tgacgcaggt ttaccaggag tcatggcagg tcacatacat ttaccaaacg
    10981 ttgaaaaaga aatgcatcca gagcgagacc ttgatgatat gttaccagct tcattgaata
    11041 aaacactttt agatgagtta ttgagaggag agttaggtta taatggagct attgtaacgg
    11101 acgcttctca tatggttgga atgactgctt caatgccaag gagagatttg ttgccaacgg
    11161 cgattgaagc aggttgtgat ttattcttat tctttaatga ccctgatgaa gacattcaat
    11221 ggatgaaaga aggttacgaa aaaggtattc taacagagga acgtctccat gacgctttac
    11281 gtcgtactct cggtctaaaa gcgaaattag gcttgcataa ctatgaggga cgtcgccaag
    11341 aactctttat gcctaaagat aaggctatgg ctttaatcaa cacattagag tcgcaaaaaa
    11401 tagctgatga agttgctgat aaagcagtaa ctctggtaaa agataaacaa aaagatatct
    11461 ttccagtaaa tccagaacgt tatcgtcata tcttacttgt taacgttgaa ggatataagg
    11521 gaggttttgg tgcgatgatt gctggaaaca agcaacgtgc atcggattac atgaaagagt
    11581 tacttgaggc acgtggccat gaagtaactg tctgggagtc taccgaagag cgtattatga
    11641 aattgccaca agaggagcgg gcagcagcta tcgctaatgt ttacgcacaa aaacagccaa
    11701 tcgcaaatct aacagaacac tatgacttga ttattaactt ggtagatgtc aatgctggag
    11761 gaacaacaca acgtattatt tggccagcag ctaaaggcac ccctgatcag cctttttacg
    11821 ttcatgaaat accatctatt gtgatttcag tacaacatgc atttgcttta gcagatatgc
    11881 cacaggtagg gacttatatt aatgcttatg atgggcttcc tagcacgata agtgcagtag
    11941 ttgcgaaatt ggctggagaa tcagaattta caggagtttc accagtagat gcttactgtg
    12001 gtttaattga tacacattta tggcgccaat gctaatagaa acaagatgat gtacttttat
    12061 gtaacataaa aaaagagctt aaagctcttt ttttatcctt caaaatagag tagttccttg
    12121 ggtgttttgg taaatacttc aaagccggtt ttagtcacat aaccgcagtc ttcaattcgc
    12181 acaccaactt tatctggtat ataaatacct ggttcaacag agaagcacat gccttcttga
    12241 atctccatat cgttaccagc cataatagat ggaaattcat ggacatccat acctaatcca
    12301 tgaccaaggc ggtggttaaa gtattgtcca taaccagcct tttcaataac atttcttgct
    12361 gctgcgtcaa cttcagaagc aaggactcct ggcttaataa aatcaatagc cgcttgatgt
    12421 gcttcaagac ataaatggta aatatctttt ttgaattgat caggtttccc cactgctact
    12481 gtacgggtca tatctgacgt ataacctagt gtttctacac ctaaatcaaa taataacaaa
    12541 gcattatttt cgattttatt agttcctggg ataccatgtg gatttgctgc attattgcca
    12601 gtcaaaacca tagtgtcgaa actcatttta ttgatacctt gttttttcat ttcaaattcg
    12661 atttgagcga taatatcggt ttcagtgttg tttagcgaga tattgtcaaa tccaacttga
    12721 acagctttat cagcaaattc tccagcgact aacattttct caatttcatc tcgtgattta
    12781 attaggcgca tgttttgaat ataaggtgtt aggttttcaa aatgaccatc aaaaacggtt
    12841 tggagacctt ggaatttagt aacattaaga ttatcaaatt cggctagaac tttagaaaca
    12901 gaaaaggatg gtaagttgct agctattttt tgccaaggat tttctgaatc tatgtaacca
    12961 aagacgggaa atggtacact ttgttttgca cgcgatactt ctagtgctgg tacaaataaa
    13021 ataggatcgc gatcttcata gacaaataaa aacatttgtc tttcatgggg atcgcaaaag
    13081 aaacctgtta aatagttgac tgtcacagga tcagaaaaga cagcaagttc agcttggact
    13141 gaatgtaagt gatggcgtat acgattaagt tttgacataa taacccttct tcctaaaagg
    13201 aacttagtct agttaggcta gtttcttatg cctattattt ttccaaaaaa ataggaaaaa
    13261 atcaagttta gttagtattt tgaaagtttt tcataaaaaa gctttataaa agcttgaaag
    13321 tgtttccaaa aggtgataaa atatcagtga aagcgtcatt ttcaaataga aaggaagtta
    13381 aatcaatatg aatacagatg atacgattac gatttatgat gttgcccgtg aagcaggtgt
    13441 ttcgatggca actgttagtc gtgtggtaaa tggaaataaa aatgtcaagg aaaatacacg
    13501 taagaaagtc cttgaagtta ttgatcgtct tgactatcgt ccaaatgctg tggcgcgtgg
    13561 tttagcaagt aaaaaaacga caactgttgg tgttgtcatt cctaatatcg caaatagcta
    13621 cttttcaatt ttagcgcgtg gtattgatga tattgcagca atgtacaaat ataacattgt
    13681 ccttgcttca agtgatgaag acgatgataa agaggttaat gttgttaata ctttgtttgc
    13741 taagcaagta gacggtatta tttttatggg acaccactta actgaaaaga ttcgtgcaga
    13801 gttctctcgt tcacgcacac cgattgtttt agcaggtact gtggaccttg agcatcaatt
    13861 accaagtgtt aatatcgatt acaaagcagc agcagttgat gttattgata ttttagctgg
    13921 caatcataaa gacattgctt ttgtatcagg accacttatt gacgatatta atggcaaggt
    13981 tcgtttggca ggatacaaag aaggtctcaa gaaaaatggc ttaaacttta aagaggggtt
    14041 ggtttttgaa gccaactacc gttatgctga aggctttgca ttagctcaac gtgtgattaa
    14101 tgctggagca acagcagcat atgttgctga ggatgagtta gcggcaggtt tgttaaatgg
    14161 tctttttgag gcaggtaaac gtgttcctga agattttgaa attatcacaa gcaatgactc
    14221 gccaatcgca caatatacac gtcctaactt aacttctatc agtcaaccgg tctatgattt
    14281 gggtgctgtt agcatgcgta tgctaacaaa aattatgcac aaagaagagt tggaagaaaa
    14341 agaaatagtg cttaatcacg ggattgttaa acgtggcaca acaaataatt agtgttagag
    14401 tatgttattt gaaaaatagt ggtagaatgg tagtagctta gcgcttaact ttctgatact
    14461 taacatgata tattgagagg attagggtag cttagcttcc taatcttttt ttagaggtgt
    14521 aatatgacaa atgaattgat aatgcaagct tttgagtggt atttacctag tgatgggaat
    14581 cactggaaga aattagagga gtctatatca gaccttaaaa aacttggaat tagtaaaatc
    14641 tggttaccac cagcatttaa gggaactagc agtgatgatg taggatatgg tgtttatgat
    14701 ctctttgatt taggagaatt tgaccagaat ggaacaatta gaacaaaata tggtaggaaa
    14761 gaagagtatc taaagcttat taagtcgtta aaggcaaatg gcattaaacc gtttgcagat
    14821 atcgttctta accataaagc caatggtgat cataaagaaa aatttcaagt catcaaagtc
    14881 aatcctgaaa atcgtcaaga agcattaagt gaaccctatg agattgaagg atggacggga
    14941 tttgatttcc caggtagaca gggtgagtac aatgatttta aatggcactg gtaccacttt
    15001 actggtttag attacgatgc taaaaataat gaaacagaca tctttatgat tgtaggcgat
    15061 aataaaggtt gggcagacga tgatttgatt gatgatgaaa atggtaattt cgattatctc
    15121 atgtacaatg acattgattt taagcatcct gaagttataa aaaacttaca agattgggct
    15181 aagtggttta ttgaaactac aggcattgaa ggttttcgtt tggatgctgt gaaacatatc
    15241 gattcttact ttattcaaac tttcattaat gatattcgta caaaaataaa acccgattta
    15301 gaggtctttg gcgaatattg gaaatctgac caaacatcaa tgaaggacta tttagaggca
    15361 acacaatttc aattttctct tgttgatgtc accttgcata tgaatttttt tgatgccagt
    15421 caccaaaacc gtgattttga tatgcgaaca atttttgatg atagtcttgt tattgataat
    15481 cctgagtatg ctgttacatt cgtagaaaat cacgatacgc aaagtggaca agctctagaa
    15541 agtagagtgg aagattggtt taaaccttta gcttatggct tgatactttt acgccaacaa
    15601 gggactccat gtctttttta tggtgattac tatggcattc aaggagaatt tggccaacca
    15661 tcctttaaag aggttattga taaaatggct gagctacgcc aaaactatgt atttggcgaa
    15721 caagtcgatt actttacgca ttcaaattgt attggttgga cttgtcttgg ggatgaggaa
    15781 cataattctt gtttagctgt ggtcctaaca aatggtgatc agggatggaa acatatggaa
    15841 gtaggggaaa tttatgcagg caaaactttt gttgattatt taggcaattg tgaacaagag
    15901 gttgttatag gagatgatgg ttggggtgat tttttagttg catctgcgtc tatctcggct
    15961 tgggtcccta aaatagagga agctaactga taaatcaggt gaaaaaaaag gtaaaatgag
    16021 atataataag acctatgaaa gttttactgt atttagaggc tgaagaatac ttgaaaaagt
    16081 caggtatcgg tcgtgctatc aaacatcaag agaaagctct tcaaattgct gggatagatt
    16141 ataccactaa tcctacagat gactttgacc ttgttcatat gaatacctat ggtattagaa
    16201 gttggctttt gatgagtaaa gctaaaaaaa ctgggaaaaa agtaattatg catggccatt
    16261 ccactgaaga ggactttaga aattctttta ttggctcaaa tttagtatcc ccgcttttta
    16321 aatggtacct ttgccgtttt tatcaaaaag cagatgccat tataacccca actgattatt
    16381 ctaaacaact aataaaagca tatggcatca agaaaccaat ttttgtttta tctaacggga
    16441 ttgatctaag tcgctatcag aggtcagaaa agaaagaagc tgcatttcgt cactattttc
    16501 acttaagtaa ggatgataaa gttgttatgg gggccggtct ttatttcatg cgtaagggaa
    16561 ttgaccaatt tgttgaagta gctgcaaaaa tgcctgatat tcgttttatt tggtttggag
    16621 aaacaaataa atgggttatt cctcgaaagg ttaggcaaat cgttaccaag caacatccct
    16681 caaatgttac ttttgctggt tacataaaag gtgatgttta tgaaggtgcc atgagtgcta
    16741 gcgatgcttt cttctttccg agtcgtgaag agacagaagg gatagtagtt ttagaggctt
    16801 tagctagtca tcagcatgtg gttttaagag atatccctgt gtatcacggt tgggtgaccg
    16861 aagacagtgt tgaattagcg actgatgttg atggttttgt tgaaaagttg gataaagtct
    16921 tatctggtaa gagtgataag attaaagaag gttatcgtgt ggctgagagt cgaagtatcg
    16981 aacgaattgc ccatgaattg gcaagtgttt atcaaaaagt aatggagttg taagatgcgt
    17041 ataggtctat ttacagatac atactttccc caagtttcgg gagtttcaac aagtatccga
    17101 actttaaagg aagggttgga gaaggaagga cacgaagttt atattttcac aactaccgac
    17161 cgtaatgtca aacgttttga agaccctaca attattcgtt taccaagtgt cccttttatt
    17221 tcttttacag atcgtcgagt agtttataga ggtttaattt ctgcttatag gatagctaaa
    17281 gactacgaac ttgatattat tcatactcaa acagaattca gtttaggact tttaggaaaa
    17341 ttagttgcaa aagctttaag aatacctgtt gtgcatactt atcatactca atatgaggat
    17401 tacgttggtt atatcgcaaa aggcaaattg attaagccaa gtatggtcaa atatatcatg
    17461 cgtacctacc taagtgattt ggatggtgtc atatgtcctt ctcgaattgt tctcaatctt
    17521 ctagatggtt acggtgtcaa aattccaaaa caagttattc cgacaggtat tcctgttgag
    17581 aactatcgtc gagaggatat ctctgaagaa acaatcaaga atctacgaac ggagcttggc
    17641 cttgcggata atgatacaat gctattgagt ttatcacggg tatcttttga aaaaaatatt
    17701 caagcaatct tgatgcattt atcagctgtc gttgatgaaa atcctcacgt aaaattagtg
    17761 atagttggag atggtcctta tctttcagat ctaaaagaac ttgttcactc tttagaactt
    17821 gaaaattcag ttatttttac aggtatggtt gagcatagcc aagtggctat ctactataaa
    17881 gcatgtgatt tctttattag tgcttcaaca agtgaaacgc aaggattaac ttacattgag
    17941 agtctggcga gtggacgtcc aatcattgcg caaagcaatc cttatctaga tgatgttatt
    18001 tcagataaaa tgtttggaac tctgtacaag aaggaaagtg atttggcaga tgctatccta
    18061 gatgcaatag cagagacacc caaaatgact caagaagctt atgaacagaa attatatgag
    18121 atttctgctg agaatttcag caagtctgta tatgcttttt acctagactt tctgatttca
    18181 caaaaagcta gtgtgaaaga aaaagtctct ctgacgatag gaaataagga tagccattct
    18241 actcttcgtt ttgtcagaaa agcggtatat ttacctaaga aagtttttac ctttacagga
    18301 agagcctcta aaaaagtggt aaaagcacct aaacgtcgta tttcatctat tcgagatttt
    18361 ttagattgag acgaataaaa ttagtaaatg tcttgaaatt ataaaaaata ttgttataat
    18421 aaaaaatgaa gacatgttag gttctaatag atttcaagag cgagcactgg ttggtggaaa
    18481 gagtgcaatt agagaactta gtactacttc cttgatttta tgtaaacata aaagggtggt
    18541 tcccttatcg ccactagagg tctgacacaa gtatactcga gtttttgtgt tggataaatt
    18601 gaggtggaac cacgtagtct ttacgtcctt ttggatgtaa aggctctttt tttataaaag
    18661 aagtggttgc ataaaagagg cagattgctt ttctaatgtt atacttatta tccctatcta
    18721 ggataatgtg gaattcaatc aaaggtcaat ccctttgttt attagtgaat taatgtgttt
    18781 aggaattggt atcaccctaa cagttattaa gaaaaggaga acaagatgat taaaatcact
    18841 ttcccagatg gtgcaattcg tgaatttgaa tctggcatca caacatttga aattgcacaa
    18901 tcaatttcta actctttagc taaaaaagct ttagctggta aatttaatgg tcaacttatt
    18961 gatacaacac gtgctatcga agaagatggt agtattgaga ttgtaacacc tgaccacgaa
    19021 gatgctttag gagttcttcg tcactcagca gcgcatttat ttgctcaagc tgcaaaacgc
    19081 ctttttccag acttatgttt aggtgtaggt ccagctattc acgatggttt ttactacgat
    19141 actgataata aatcaggtca aatttcaaat gatgatcttc ctcgtattga agaagaaatg
    19201 aaaaaaattg ttaaggaaaa tcacccttgt attcgtgaag aaatcagtaa agaagaagcg
    19261 ttagaacttt ttaaagatga cccttataaa gttgaattaa ttagtgaaca tgctgaagat
    19321 ggtttaactg tttatcgtca aggtgaattt gtagaccttt gtcgtggacc tcatgtccca
    19381 tcaacaggac gtattcaagt ctttcatctt ctaaatgtcg ctggggctta ctggcgtggt
    19441 aacagtgata atgctatgat gcagcgtgtc tatggtactg cttggtttga taaaaaagat
    19501 ttgaaagcat atcttaaacg ccgtgaagaa gctaaggaac gtgaccaccg caaattaggt
    19561 aaagaacttg atttatttat ggttaatcct gaagtaggtc aaggattgcc attctggttg
    19621 ccaaatggag caactattcg ccgcgaattg gaacgttaca ttgttgataa ggagattgct
    19681 tcaggttatc aacacgttta cacacctccg atggcatctg ttgaatttta caagacttct
    19741 gggcactggg accactatcg tgaagatatg ttcccaacaa tggatatggg agatggggag
    19801 gaattcgttc ttcgtccaat gaactgccca caccatattg aagtttataa acatcatgtt
    19861 cactcatatc gtgaattgcc aattcgtatc gctgaacttg gtatgatgca ccgttatgaa
    19921 aaatcagggg ccctcacagg attgcaacgt gtacgcgaaa tgacactcaa cgatgcccat
    19981 attttcgtta cacctgagca aattaaagat gaatttctta aagctcttaa tctcatagca
    20041 gaaatttatg aagattttaa cttaacagat taccgtttcc gcttatctta tcgtgaccca
    20101 gaagataaac ataagtatta tgataacgat gagatgtggg aaaatgcaca agctatgcta
    20161 aaagaggcta tggatgattt tggattagat tattttgaag cagaaggcga agcagccttt
    20221 tacggtccta aattggatat ccaggtgaaa actgctcttg gtaatgaaga aacattatct
    20281 actatccaac tagatttcct tcttccagaa cgctttgatc ttaaatacat cggtgcagat
    20341 ggtgaagaac atcgtccaat tatgattcac cgcggtggta tttcaacaat ggagcgattt
    20401 acagctattt taattgaaac ttacaaaggt gcctttccaa cttggcttgc tccacaacaa
    20461 gtttctgtta ttccaatttc aaatgaagcc cacattgatt atgcttggga agtggctcgt
    20521 gcccttaaag atcgtggtat tcgtgctgaa gtggatgacc gtaacgaaaa gatgcaatat
    20581 aagattcgtg cggcgcaaac ccaaaaaatt ccatatcaat tgattgttgg tgataaggaa
    20641 atggaagaaa aagccgtaaa tgtgcgtcgc tatggaagta aagcgactga aacaaaatct
    20701 atagaggaat ttgtagaaag tattttagct gatattgcaa gaaaatctag acctgacgaa
    20761 gttaaataaa ttttattagt cattataaaa atcattaata aatgatgtga actgattact
    20821 gtatgatgca gaatcagttt tttagatgct aattttttct tgatgtttaa ttataagcta
    20881 attatagata aataatctca tataacattc acaaaattag attttttggt ataattaata
    20941 tgattgacac ataaaggaaa atagtattaa taaaaagggg tatggagcat tgcgaacagt
    21001 attagtagtg caaggtgatg acgaaacaat agagttatta aggtcgtatt tagagggagc
    21061 tctttataag gttgtgatgg ctagtgatgg tgaggaggct ttttctttat ttcagcaaca
    21121 tcagattgat ttagcaatca tagatattac gctacctaaa atagatggat atgagttgac
    21181 gcgactcatt cgtcaagata gtcagattcc tattattatg ctagcagcta agacaacaga
    21241 tatggacaga atattaggat tgaatattgg agcagatgat tttatcacga aaccgtttaa
    21301 ttctttggag gttttagctc gaattaattc gcaactgcgt cgttactatg aattcaatag
    21361 tcttgccaaa cctaaaaatc aatttataaa gataggggaa ttggaactag atgaggaaca
    21421 tgttgaacta actaaaaatg gtaaacatat caagttgact gctacagaat ttaagattct
    21481 tcatatctta atgtcatcac caggaaagat ttatactaaa acacaactct atgagaagat
    21541 taatggtagg tatttagaag gtgatgagac aacgattatg gtgcatattt ctaatatccg
    21601 tgacaaaatc gaagatgata gtaaatatcc taaatatata aaaacactta gaggagtagg
    21661 gtataaaatc gaaaaaccgc agtatcttgt ttaaaaacat aatggctagt gagagccatt
    21721 ttttagtgaa gatatttctt ggttttaagc tttccttttt gctataataa gtaaaattgg
    21781 aggggagaga tatggcaaag accgctctta atattccttt tacagttgaa gggatagaaa
    21841 attattatca aaatataaaa atgatactgt ctttagcaga taaatggagc atacctaaag
    21901 atgatctatt actcttttta aagggactca aggctattga tgatataaaa gttggctctc
    21961 ctatttttaa gtatttttgg acttctttaa gtttacaagc acccctcaaa gctttagaat
    22021 tcgtgttaga gcaggctaaa atgccaactg aattgtcagg agaattatca gaaacacaat
    22081 acttggtcgc acagttttca gatgagttag ctccgcatga tgatttctgg atagccttat
    22141 cacaagttat ttatgattct tttcctggga atagtctagc cgaagatact gtattaaata
    22201 ggaaactcca tcaattccgc tatttgattt ctagtcagca agcacagtat gttcgacgat
    22261 actttaaaga tgttggtatg actgatagag atgctttagt aaactaccta tcatgtttaa
    22321 gagaaccaga tagtatcgcc tactatgaat ctgctcgctt gcataataaa cgtcggagaa
    22381 atggtgaaat ctttggtttc ccagatgatg agccggttat taatagtaag cttttaattt
    22441 cattccatac ggaatttatt attgatgaca aaggaaactt tttaaacgag attgacgcag
    22501 aagtaatcac acgtaatggc attatcaacg gtgctagctt taattatgct tttaaaaata
    22561 atacacgtca taaagaatta gatgttgatc ctgtaaagtt agacccaaaa tttcgaaacg
    22621 atatgactag aggttataga tcgccaaatc tatctagaag gaaatggttt ttctttaaag
    22681 aagaggatta cgattgtagt tatttcaata aaaaaggata ttacgcattt ggaaggcgta
    22741 gtgctaagca atcggttgat aaacaagtca aatacttgaa gaaggctgtg cagaaaatgc
    22801 gcatcaactg atacatatac tattaaaaga taccatgata ctatggtatc ttttaatttg
    22861 cggtattaat gtcaaatggt ttttattgac cacatatttt tataaacgag tggtcaaaat
    22921 tgaaaaagtg tctagcttat gttaaaatac aattgaataa tagaaagaag gtgctgtatg
    22981 attaagaaaa ataaagtttt tttaggagtt cttttagtcc ttgttgttat ccttggaggt
    23041 ggtgtattat tttaccaatc acaattccaa aagacaacta atcaagcgtt agctattgct
    23101 tataaagatg ctaaagtggc taagaaggat gtcatacatc aaaaaattga caaagaattt
    23161 gaaaacttta gagggagcta cgaaattgaa ttcaatacca aaagcgcaga atacagctat
    23221 catgtagatg ctaagactgg acaaatcctt gagcgggaca tggataataa tggcttttca
    23281 aaatcgactt ctcagtcaag ttcatcgtca agtcaaaaat ctcataaaat aagtcaagaa
    23341 gaagccaaaa agattgcatt taaagatgct aatatagagg aatcagaagt cagtaatctt
    23401 aagatcaaag aagaaattga aaatggaaaa tcagtttatg atattgactt tgtggacctg
    23461 aaaaataaaa atgaagttga ttatcaaatt gatgcagaaa ctggaaaaat cattgaacgt
    23521 tctagagatc atatgaatga ttaatttaaa taagatataa aaaaacgaag gtctaaaagg
    23581 ccttcgtttt aattactcag tagcttgctc ccaacgtttt gaaagtttac gtgaaaaact
    23641 tgagatgata aagtttgtaa tgaaatagac agcagttata atagcgtaga gtgtaaaaac
    23701 ttgtccagct tcgaaatatc ttcccattag aatttgactt tttccgaaga tttcttgaat
    23761 agctataaca gaataaagta gtgatgtatc ttttataacc gttacaaact gagaaataat
    23821 ggctggcaac attttgcgaa aagcttgtgg aaaaataata ataatgaaaa cttgcaaata
    23881 agtgaatcct tgagaaagtc cagcttctgt ctgtccttta tcaactccat ttaaacctcc
    23941 ccgaataatt tctgctaaag cagcggaggt aaaaattgta aacgaagtaa taccagcgct
    24001 gactgatttc atctggaata ctaagaaaat gataaaaatc caaagtagat ttggaacatt
    24061 tctgacaaat tcgatataga tactagctat taatttccag ataggatttt tgctatttct
    24121 cataattgct aatagagttc caaaaaacat tgatagtaaa atggaaataa aagatatata
    24181 taaggttaaa ccgaaccctt gaaggagaaa tagtatattt gttagtgtta agactgatga
    24241 catatcaaac ctccttaaga ataagttttc ttatttaatt cttcttgttt tcttgcccag
    24301 cttgctagtg ggaaacaaat aacaaaatag aagatagcag ctcctgcaaa agctgggata
    24361 taattagtcg tatcataagc ccaggcctta gctaaaaaca tgatatcggc tccagaaatg
    24421 attgctacgg tagaggtatt cttgattagg ttaacaactt ggttggtcat tggtggtaaa
    24481 attgttctga tagcttgagg caaaatgata agttgcatgg tttgattagc tgtgaatcct
    24541 tgagctaaag cagcctctgt ttgtccttta gggacagctt caattcctga gcgaataact
    24601 tcagaaatat aggcaccatg gtataggcca acgcacaaaa cagcggtgaa aaaagcacta
    24661 atcataatat gaccattact aatgattgct agtccatagt agacaaaaac catttgaacc
    24721 aaaagaggtg tgttttgaaa aacttcaaca tagatacgtg atatcaactt accaacttta
    24781 cttttagaag ttgataaacc accagagaga attcctaata tcagagcaag taggagtgca
    24841 ccaaacgaaa tacctagagt atataagaag cctttaaaga ataggtcaaa atggttaaag
    24901 aaagcgcccc accttgaaat ggcaaaagga ctgatgttta ataaagtcat aaaaaatcct
    24961 ccctattctt tttttacttt agcgggttta agcttaaatt tttgataaat gtgttttaaa
    25021 ctaccatctt tttcccatga taccagtaac ttattaacgt agggaatcaa gactttgtcc
    25081 tgtttactgg ttgcaatacc gtattcttgc gtattgaacc cttcttttaa tatggttgta
    25141 tgagggctaa ggtagccgct aaggatagat ttatcaactg aaaaagcatc aatacgatta
    25201 gcgtacagcg atatcgctaa ttctgggaaa ctccctaatt gagcatagct aaattttagc
    25261 ttgtgttctt tagcatattt ttctaaattg acttttgtag tggcaccttg agccacacca
    25321 atgtgtttac catccaattc ttttatagtt ttgatatgac tatttttacg caccaaaaat
    25381 ccaatttcat cgtgataata agctttagaa atattatagt tagcttttct ttcgggcgtg
    25441 atagtgtatg ttgcgattag aatatcaatt tgcccattgt ccatcagagg ctctctggtt
    25501 tgtgcagttg tcggtacaaa aactggtttg acgcctaagg atttggcaat ttttttggca
    25561 atatcaattt ccattccttc gtattgattc gtctcagcat tataataacc aaagttaggc
    25621 acatcttgct tgactccaac tttaaggaca cccgattttt tgatttctga taattgatta
    25681 gctgacgttg agttgggata aaataacagg aaaatgaaaa tacaggataa tagatagagc
    25741 gttaatcttt ttcgtctcag catatttcgt cctcctaaag gtcagtactg gtatggttga
    25801 taatgtttct taaaaattgt ttggcacgtg gttctgtggg attatcaaaa aagtcttgca
    25861 catccgtagt atctactaaa atttccccgt cagccataaa aataatacga tcagcaactt
    25921 ctcgtgcgaa tcccatttca tgagttacaa cgaccatatt cattccgtca tttgccaatt
    25981 tttgcatcac tgaaagaacg tctccgatgg tttcgggatc aagggcagag gtaggctcat
    26041 caaagaggag gagttttgga tgcatggcaa gccctctagc aatagcaatt ctttgttttt
    26101 gaccacctga tagcatagat gggtaggaat cttttctttc ccacatattg acaaatttta
    26161 aatatttttc agcaatttcc atagcttctt ttttagattg ttttaatact ttaataggag
    26221 ctaatgtaat attttctaac acggttttgt ggggataaag gttaaaatgt tgaaaaacca
    26281 taccaacttc tttacggagg ttgaccaatt ctttagatga aatatttgct aattcatgtc
    26341 cattcacgac caaactacca tcgtcaatag attcgagagc gttcattgtt ctaatgagtg
    26401 ttgatttacc tgatccagaa ggtcctaata gaacaaccac ttgccctggc tctatttcca
    26461 aattaatctg acgaagggcg tgataatctc cataatactt gttaacattc ttataagata
    26521 tgagtgacat aagaacctcc taaaataaca gtttattgtt aggttaccta acattataga
    26581 taagtgtatc acctgtttgt gagcttgtca ataaattcag aatattaaag agtaggattg
    26641 atttaaacat tgctattt
//
LOCUS       DATRNX010000015        26423 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRNX010000015 DATRNX010000000
VERSION     DATRNX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26423
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(350..736)
                     /gene="rplQ"
                     /locus_tag="VBC81_000511"
     CDS             complement(350..736)
                     /gene="rplQ"
                     /locus_tag="VBC81_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6911573.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(751..1689)
                     /locus_tag="VBC81_000512"
     CDS             complement(751..1689)
                     /locus_tag="VBC81_000512"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6911574.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1739..2122)
                     /gene="rpsK"
                     /locus_tag="VBC81_000513"
     CDS             complement(1739..2122)
                     /gene="rpsK"
                     /locus_tag="VBC81_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6911575.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2140..2505)
                     /gene="rpsM"
                     /locus_tag="VBC81_000514"
     CDS             complement(2140..2505)
                     /gene="rpsM"
                     /locus_tag="VBC81_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6911576.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2523..2639)
                     /gene="rpmJ"
                     /locus_tag="VBC81_000515"
     CDS             complement(2523..2639)
                     /gene="rpmJ"
                     /locus_tag="VBC81_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6911577.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2665..2883)
                     /gene="infA"
                     /locus_tag="VBC81_000516"
     CDS             complement(2665..2883)
                     /gene="infA"
                     /locus_tag="VBC81_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6911578.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2999..3637)
                     /locus_tag="VBC81_000517"
     CDS             complement(2999..3637)
                     /locus_tag="VBC81_000517"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6911579.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3732..5036)
                     /gene="secY"
                     /locus_tag="VBC81_000518"
     CDS             complement(3732..5036)
                     /gene="secY"
                     /locus_tag="VBC81_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6911580.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5057..5497)
                     /gene="rplO"
                     /locus_tag="VBC81_000519"
     CDS             complement(5057..5497)
                     /gene="rplO"
                     /locus_tag="VBC81_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6911581.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5622..5801)
                     /gene="rpmD"
                     /locus_tag="VBC81_000520"
     CDS             complement(5622..5801)
                     /gene="rpmD"
                     /locus_tag="VBC81_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6911582.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5816..6310)
                     /gene="rpsE"
                     /locus_tag="VBC81_000521"
     CDS             complement(5816..6310)
                     /gene="rpsE"
                     /locus_tag="VBC81_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6911583.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6329..6685)
                     /gene="rplR"
                     /locus_tag="VBC81_000522"
     CDS             complement(6329..6685)
                     /gene="rplR"
                     /locus_tag="VBC81_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6911584.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6786..7322)
                     /gene="rplF"
                     /locus_tag="VBC81_000523"
     CDS             complement(6786..7322)
                     /gene="rplF"
                     /locus_tag="VBC81_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6911585.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7432..7830)
                     /gene="rpsH"
                     /locus_tag="VBC81_000524"
     CDS             complement(7432..7830)
                     /gene="rpsH"
                     /locus_tag="VBC81_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6911586.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7985..8170)
                     /locus_tag="VBC81_000525"
     CDS             complement(7985..8170)
                     /locus_tag="VBC81_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6911587.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8188..8730)
                     /gene="rplE"
                     /locus_tag="VBC81_000526"
     CDS             complement(8188..8730)
                     /gene="rplE"
                     /locus_tag="VBC81_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6911588.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8754..9059)
                     /gene="rplX"
                     /locus_tag="VBC81_000527"
     CDS             complement(8754..9059)
                     /gene="rplX"
                     /locus_tag="VBC81_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6911589.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9139..9507)
                     /gene="rplN"
                     /locus_tag="VBC81_000528"
     CDS             complement(9139..9507)
                     /gene="rplN"
                     /locus_tag="VBC81_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6911590.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9532..9792)
                     /gene="rpsQ"
                     /locus_tag="VBC81_000529"
     CDS             complement(9532..9792)
                     /gene="rpsQ"
                     /locus_tag="VBC81_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6911591.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9818..10024)
                     /locus_tag="VBC81_000530"
     CDS             complement(9818..10024)
                     /locus_tag="VBC81_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6911592.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10034..10447)
                     /gene="rplP"
                     /locus_tag="VBC81_000531"
     CDS             complement(10034..10447)
                     /gene="rplP"
                     /locus_tag="VBC81_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6911593.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10451..11104)
                     /gene="rpsC"
                     /locus_tag="VBC81_000532"
     CDS             complement(10451..11104)
                     /gene="rpsC"
                     /locus_tag="VBC81_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6911594.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11117..11461)
                     /gene="rplV"
                     /locus_tag="VBC81_000533"
     CDS             complement(11117..11461)
                     /gene="rplV"
                     /locus_tag="VBC81_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6911595.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11477..11755)
                     /gene="rpsS"
                     /locus_tag="VBC81_000534"
     CDS             complement(11477..11755)
                     /gene="rpsS"
                     /locus_tag="VBC81_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6911596.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11854..12687)
                     /gene="rplB"
                     /locus_tag="VBC81_000535"
     CDS             complement(11854..12687)
                     /gene="rplB"
                     /locus_tag="VBC81_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6911597.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12705..13001)
                     /locus_tag="VBC81_000536"
     CDS             complement(12705..13001)
                     /locus_tag="VBC81_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6911598.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13001..13624)
                     /gene="rplD"
                     /locus_tag="VBC81_000537"
     CDS             complement(13001..13624)
                     /gene="rplD"
                     /locus_tag="VBC81_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6911599.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13648..14274)
                     /gene="rplC"
                     /locus_tag="VBC81_000538"
     CDS             complement(13648..14274)
                     /gene="rplC"
                     /locus_tag="VBC81_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6911600.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14347..14655)
                     /gene="rpsJ"
                     /locus_tag="VBC81_000539"
     CDS             complement(14347..14655)
                     /gene="rpsJ"
                     /locus_tag="VBC81_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6911601.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14863..16143)
                     /locus_tag="VBC81_000540"
     CDS             complement(14863..16143)
                     /locus_tag="VBC81_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6911602.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16248..17738)
                     /locus_tag="VBC81_000541"
     CDS             complement(16248..17738)
                     /locus_tag="VBC81_000541"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6911603.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17858..18874)
                     /gene="adhP"
                     /locus_tag="VBC81_000542"
     CDS             complement(17858..18874)
                     /gene="adhP"
                     /locus_tag="VBC81_000542"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6911604.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19053..21695)
                     /gene="adhE"
                     /locus_tag="VBC81_000543"
                     /gene_synonym="adhC"
     CDS             complement(19053..21695)
                     /gene="adhE"
                     /locus_tag="VBC81_000543"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6911605.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21964..23742)
                     /locus_tag="VBC81_000544"
     CDS             complement(21964..23742)
                     /locus_tag="VBC81_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6911606.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23739..24119)
                     /locus_tag="VBC81_000545"
     CDS             complement(23739..24119)
                     /locus_tag="VBC81_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911607.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24126..24563)
                     /locus_tag="VBC81_000546"
     CDS             complement(24126..24563)
                     /locus_tag="VBC81_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6911608.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQITYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24715..25713)
                     /gene="ruvB"
                     /locus_tag="VBC81_000547"
     CDS             complement(24715..25713)
                     /gene="ruvB"
                     /locus_tag="VBC81_000547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6911609.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(26010..>26423)
                     /locus_tag="VBC81_000548"
     CDS             complement(26010..>26423)
                     /locus_tag="VBC81_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6911610.1"
                     /translation="FLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNH
                     VLLNCVFVGLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKS
                     AENAYQQSIMFSKMIDDKHLIKKLELEWQEDITGH"
BASE COUNT         7985 a         5455 c         4482 g         8501 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gtattttata aatttagtat
       61 agacataact attcctctat attcaattaa gagaaattat ataaccacta ttgagaaatg
      121 tagtctagct attaaactct ttgctcatcg aaaatatcca ataaatataa tacataaaac
      181 gcctgcttac gaaatataaa caaaattgtt tgcatttcgt aaacaagcgt tacctattta
      241 acaatatatg atgagtgttc ccgctgagaa taattctcag cggtagacca gagctagact
      301 aagaatcgat tgattccatc atcataacac tcaacaaaat tgataaaaat tatactaatt
      361 caataattgc cattggggca gcatcgccac ggcgtggttc tgttttaaga atacgagtgt
      421 atcctccgtt acgttcagca taacgaggtg cgatgtcgtc aaaaagtttt tgaagagctg
      481 ttgttgatgt atatttatca gaagcttcat catagttttc tgatgcaatt tcattacgta
      541 cataagcagc agcttgacga cgagcatgta aatcaccacg tttacctaga gtaatcattt
      601 tttcaactgt tttacggatt tctttagcac gtgcttcagt agttacaatt gattcgttga
      661 taagaagatc ggttgtcaaa tcacgaagca ttgccttacg ttgtgagcta gtgcgtccta
      721 gtttacggta agccattatg tcctcctatt ttatttatcg ttttttaatc caagacctag
      781 atcggcaagt ttgattttaa cttcttcaag actcttacgt cctaagtttc ggactttcat
      841 catttcaggc tcagtttttt ctgttaaatc aaatactgta ttaattccag cacgttttaa
      901 acagttatat gagcgcactg acaaatcaag ttcctcaatt gtgcggtcaa gcactttctc
      961 atcgttcact ttctctgttt ccttcattac ttcagttgct ttagcaacct ctgttaaatc
     1021 agtaaacaag tttaagtgtt caattaagac gcgagctgaa agaccaagag catcctcagg
     1081 aatgattgtg ccatttgtca tgatttcaat agttaattta tcaaagccat cattactacc
     1141 tacacgagca ggttcaactt gataattaac ttttttcact ggtgtataga ttgaatctac
     1201 agccaatgtt cctactggtg catcatcttt tttatttcct tctgctggaa cataacctcg
     1261 gtttttagca acggtcattg ttgctttcaa actgtgacct tcagcaattg tgaaaagata
     1321 atggtcaggg tttacaattt cgatatcact atctgttaaa atatctccgg cagtaatttc
     1381 tgcaggacct tcaacgtcta gttcgataat cttttcgtct tcgacgtaag atttcacagc
     1441 aagtcctttt acattaagga tgatttgcat cacatcttca cgtacacctg ggatagtatc
     1501 aaattcgtgt aatactccat caattttgat tgatgtgact gctgcacctg gaagtgaaga
     1561 cagaagcaca cgacgaagag agtttcctag agttgtaccg tagccacgtt ctagtggttc
     1621 gatgacaaat ctgccgtaat ctttattttc atcaattttt gttattattg gtttttcaaa
     1681 ctcaatcatt tagttactcc ctcttaaacg aaaagttgtg tactataaaa tgctatgatt
     1741 atacacgacg acgttttgga ggacgagcac cattatgtgg tacaggagtc acatcacgaa
     1801 ttgcagttac ttcaagacca gctgcagcaa gggcacggat agcagattca cgacctgaac
     1861 caggaccttt tacagtaact tcaacagttt taagaccgtg ttcttgtgca gatttagcag
     1921 cagcttcagc agccatttga gcagcaaatg gagtagattt acgagaacct ttaaatccaa
     1981 gagcaccagc tgatgaccat gcaagagcgt taccatgcac atctgtaatc ataacaatag
     2041 tgttattgaa tgtagcgtga atatgagcaa caccagattc gatgttcttt ttcacacgac
     2101 gtttgcgtgt tggtttagcc aattgtttta cctcctattt tattttttct tacctgcaat
     2161 cgcaacagct ttacctttac gagtgcgagc gttattttta gtattttgtc cacggacagg
     2221 aagtccacga cggtgacgga ttccacggta tgaaccgatt tccatcaaac gtttaatatt
     2281 caagtttacc tcacgacgta ggtcaccttc aactttgatt gcatcaactt cacgacggat
     2341 agcgtcctct tgatctggtg tcaaatcttt aacgcggata tcttcagaaa tacctgcagc
     2401 tgctaagatt tttttagatg tagaaagacc aattccgtat acataagtaa gtgaaattac
     2461 tacacgtttg tcatttggaa tatcaactcc agcaatacga gccattttat ctcctttcta
     2521 tattatcctt gacgttgttt gtgttttgga tttgtcggac aaattaccat aacacgaccg
     2581 ttacgacgaa taactttaca gtattcgcaa attggtttaa ccgatggtct taccttcatg
     2641 ttttaatccc tccaattatt tcgactattt aaagcggtat gtgatgcgtc cacgtgttaa
     2701 atcgtatggg ctcatttcaa cagtaacacg atcacctacc aaaatacgga tgtagttttt
     2761 acggattttt cctgatacag ttgccaaaat ttggtgtcca ttttccaact caacagtaaa
     2821 cattgcgtta ggcatagttt caacaacttt accttcaatt tcaatcacat cttcttttgc
     2881 cacgcaaaag taccccctaa atttcgattt gatgtcctct ataaagaggt aacaattata
     2941 agtcagacta acttagtata tcattactaa tctacttatg caagtagatt agcataattt
     3001 atttgagttc tagcaacgct ttttcaacat ctgcaaaaac ttctgttatt tcttgattac
     3061 cttcaatatc tgtaacaaga ccaagcttac gatagtgttc aagaataggt tctccttgag
     3121 caatattaac gtccaagcga cgtttgacag tttcaggctt atcatcttca cgttgatagt
     3181 aatcttcttc tttataatct actggtgggt tgaacacttt gtggaaagtt tcaccagttt
     3241 tacgattgat aatacggcca ctcaaacgct ctataaggca tgttggatcc actttaatat
     3301 taataacacc atctaagcgt agtcctagtt cttcaagcgt agcatctaag gcgtgtgctt
     3361 gctcaatagt acgtggatat ccatcaagta aaaaaccttt ttctgcgata tcatcctcag
     3421 ctaagcgctc ttttacaatc ccgtttgtta cttcatcagg aaccaattca cctttatcaa
     3481 tataactttt agctaaacgt cccatttggg tttgattagc cattgcggcg cggaacatat
     3541 cccctgttga gatgtgagca acaccaaatt cttcaacaat cttagctgct tgagtacctt
     3601 taccagcacc aggcaaaccc ataattaaaa gattcattat agtctcctta ttttgttttt
     3661 aaataaatcc aacacagcat gtcggactta ttaaaaaaca aaatagaagg ttgacagagt
     3721 caacctatat tctattctgt tgtattcatg aatccaacat agcgtctctt caaaagatag
     3781 ccctcaagtt gtttcatacc ttcaatacct gttgcaatca aaatgagtaa gcttgtacca
     3841 cctaacgcaa tacttgatga caaatgcaac gcttgttgag caataattgg taacaaggaa
     3901 ataaatgcta gaaatactga accaattgtt gctaattttt tcaatagcga tgacatatac
     3961 tcttctgttt ctcgtcctgg tcgaatactt ggaatgtagg acgaattttt ttgaagattc
     4021 tctgctgtct tttcagggtt tacttgtaca aaagtataga agaaagagaa cagaataatc
     4081 aagatagcat agataatcat accgactggg gtttgataat taagtaattc ttggagttta
     4141 gtaagccaag gaatttcttt accattttga aagaaaggaa taatcgtact tggaattgtt
     4201 gtaatcgagc tagcaaagat aacgggaata acaccagctg gatttacttt tagaggaaga
     4261 tatgaacttg taggagctcc ttgaacaagt tttgtatatt ggattggaat tttgtattca
     4321 gcttgttgaa taaatgttgt aaaaaataca attgctaata ctgctacaat aagaatcccg
     4381 acaaagatgt aagaatttgt aattgctgaa gagcgtacat ttacaaagaa atcttcgtag
     4441 atagttgtaa ttgcacttgg aatggaagaa atgatccccg caaagataat catagacact
     4501 ccatttccga atcccttatc agtaatttgt tcacctagcc aagtaaccac catactacct
     4561 gttgtaagga ttgcaccaat aagcaaatat gtttgtacat tcggagtttt aactaaagca
     4621 acacttgata gagtgttaaa accagcagta ataccaattg attgtacgaa tgcaaggaac
     4681 aaagaaatat aacgtgtagc ttggttaagc ttacgacgac ctacctctcc ttgttttccc
     4741 cattcaacaa acttcggaag aatatccatt tgaagtagct gaacaacaat agacgcagta
     4801 atgtatgggc taactcccat agagaatacc gaaaagtttc tcattgcatt accactaact
     4861 aagttaagca tatttagaaa tggaagttca cccatttgtt ctaaactctt aacattgatg
     4921 cccggtactg tgatatgcgt tccgatacga aatacaagaa ggataaagat agtaaataaa
     4981 attttatttc ttaccatctt tactttgagt gcatctctaa gtaattttaa aaacataatg
     5041 agttacctct cattaattag atgacttcga ttgaaccacc tttagcagtg atagctgctt
     5101 cagcagattt agaaaattta gctgctttaa cagacaattt cttggttaat tcaccattac
     5161 caagaatttt aacgcctgat ttttcagcac gcacgattcc agcttcttta agaacaactg
     5221 gagttacttc agtaccatct tcaaagacat ttaattggtc aaggttcaca agagcatatt
     5281 cttttgcatt gatgtttgag aacccacgtt ttggcatacg acggaacaat ggagtttgtc
     5341 caccttcaaa accaaggcgt actccaccac cactacgcgc tttttgtcct ttttgaccac
     5401 gtccagaagt tttaccatta cctgaagatg taccgcgacc cacacgattg cggactttac
     5461 gtgatccttc tgcaggtttt aattcatgaa gtttcattat tttttctcct tatttgtaaa
     5521 atgctaacgc ctttacgagg ggaaaggtac tccccgcaaa gactcgccta tacgatttgt
     5581 ttaaatcgca ccaataacaa tcagtgcgat ttagctttta attaagcttc ttcaacagtt
     5641 actaagtgag agatagcgtt aaccatacca cggatggcag cattatcttc cttaacaact
     5701 gaagagttta atttaccaag tccaagggca acaactgttt tacgttgttc tggtttgcga
     5761 ccgattggag acttagtcaa agtaatttta atttgagcca ttataatccc ctttcttatg
     5821 ctaagtcaga aacagaaatg ccacgtaaag cagcaacttc ttctgcacgt tttaattgtt
     5881 tcaagccctc aacagttgcg cgaacaatgt tgattggagt gtttgaacca agtgatttag
     5941 atgtaatatc tgcaacacct gctaattcga tgacggcacg aacagcacca ccagcggcaa
     6001 ccccagcacc ctcaacagct ggtttcaaca atactttcgc tccaccaaat tctgaacgaa
     6061 cttcgtgagg aattgttgta ccgaccatag gaacttcaac catgtttttc ttagcagctt
     6121 caacagcttt gcgaatagct tctggtactt cttgagcttt acctgtacca aaaccaacac
     6181 gaccattacg gtcaccaaca actacaagag ctgcaaaacg tagacgacgt ccacctttaa
     6241 caacttttgt aacgcggtta atcgcaacaa cgcgctcttc aagttctact gcattatctt
     6301 taaatgccat tattaagtgt cctccctatt agaatttcaa tccgttttca cgagctgagt
     6361 cagccaaagc ttttacacgt ccgtgataga gatatccacc gcggtcaaac accacttcag
     6421 aaataccttt agctactgcg cgttcagcaa caagtttacc gacaacaacg gcttgttctg
     6481 tttttgttcc gttagaaact tctttatcaa gagttgatgc acttgcgagc gttacacccg
     6541 ctacgtcatc aattacttga gcgtagatgc ctgtattaga acggaaaatg ttcaaacgtg
     6601 ggcgatcagc agttccagag agtttaccgc gaacgcgacg gtggcgtttt tggcggattt
     6661 tatttttatc tggtttcgaa atcacaattt tcacctctta aatatattgt tatgtcaaaa
     6721 tgacaatatt aaaatgaaaa gatagagaat ttgcagaaaa gattctacaa aatctatcaa
     6781 tcctattatt tacctgtttt accttcttta cggcgtacga attcaccaac gtaacgaata
     6841 cctttacctt tatatggttc tggtgaacgt aagctacgaa cgtaagcagc agtttgtcca
     6901 acgctttctt tgttgatacc gattacgttg attgttgttg gagttggcac ttcaaaagta
     6961 acaccttctg gagcttcaac ttcgtcttgg tgagacttac caacagaaag aacaagtttt
     7021 gatccttgaa gttgtgcacg gtaaccaaca ccgcgcattt caagagcttt tttgaaacct
     7081 tcagaaacac caacgaccat gttgttcaag ttcgcacgag ttgtaccatg aattgttttc
     7141 atttcttttg agtcgttagg acgagtaact gttacttcag tcccttcaac agtgattcca
     7201 atatttttat taaactcacg agtgagttcg cctttaggtc cttttacagt aacaacatta
     7261 tccttgttaa tgatttcaac acctgcaggc aatgtaatta ctttattacc aatacgtgac
     7321 atgtttattt tctcctgtta aattgtcaag ctgcttgagc aactagtttt cacggggact
     7381 catgagacca caggattata ctgtgactca atgagcattt taggtaaaat tttaccaaat
     7441 atatgcaagt acttctccac caacattttt ttggcgagct tctttgtcag tcaaaagacc
     7501 ttcagaagtt gatacaattg caataccaag tccgttaaga actttaggca tatcttcatg
     7561 tttagtataa acacgaagac ctggttttga aatacgtttc aagttagtga taacacgttc
     7621 accattttgt ccatatttaa ggaacacacg gatgatgcct tgtttgtcat cttcaataac
     7681 ttcaacgttt tttacaaaac cttcacgttt aaggatatca gcaatccctt ttttaatgtt
     7741 tgatgcaggt acttctaaca cttcatgttt tgcttggtta gcattacgaa tacgagttaa
     7801 aaagtctgca attgggtcag tcataaccat tttttaattc tcctcttatt agcagtttga
     7861 atttaatcac ttgctaatta atgagtcctt acggacgttg taagactagg tcttaatata
     7921 tttgcgttta aaaatcagtg ccattatcaa tgacaccttt taaacacaaa gcatcattaa
     7981 tttattacca agatgcttta gttacgcctg gtacttgacc tttataagcc aagtcacgga
     8041 agcaaacacg gcaaagttgg aatttgcggt aaactgagtg tggacgtccg catttttcac
     8101 aacgagtgta agcttgcgta gagaacttcg ctgggcgttt gttcttagca atcatagatt
     8161 ttttagccaa tttatttacc tcctagatta ctttgcaaaa ggcattccaa ggccttttag
     8221 caattcacgt gactcttcat cagtgttagc agtagtaacg ataacgatat caagaccacg
     8281 tactttatca acgtcatcaa agttgatttc tgggaagatc aattgttctt taactccaag
     8341 agtgtagttt ccacgtccat caaatgattt cgttggaaca ccatggaagt cacgtacacg
     8401 tggaagtgaa acagaaacta atttatccaa gaattcgtac atacgttcac cacggagggt
     8461 aacttttgca ccgatcgcta caccttcacg aagacggaag ccagcgattg atttcttagc
     8521 tttagtaata agtggttttt gacctgaaat aagtgctaac tcagcagcag ctttctcaag
     8581 atttttagcg tttgatacag catcaccaac acccatgtta agaacgattt tctcaacttt
     8641 tggcacagcc ataacagatg aatagttaaa tttttctgtc aacgcaggaa caacttcatt
     8701 agtatatttt tcttttaagc gatttgccat tatgcttctc ctttccttcg tgattaatca
     8761 agcacttcgc ctgatttttt gttgtaacga acttttttgc cgtcaacaac tttgtaacca
     8821 acacgtcctg ctacaccatt tttatcaaga acttgaacgt ttgacacatg gattggagct
     8881 tctttttcaa cgatagcgcc ttgagggttt tcgttatttg gtttttgatg tttcttgatg
     8941 agggctacgc cttcaacaac aactttgttt acttttggaa gagctttaag aactacagct
     9001 tcagtgcctt tgtccttacc agcaataacg cgaactttgt cgcctttttt tacaaacatt
     9061 tgagtttttc tcctattttt cttacgcctc gaaaggcacc ctaggaaatc ctaggggact
     9121 acgtttgtta atctgagatt aaagtacttc tggtgccaat gaaacgatct tcatgtagcc
     9181 accttcacgc aattcacgtg caactgggcc aaagatacga gttccgcgag gagttttatc
     9241 gtcacggatg attacagcag cattgtcgtc aaatttgatg tatgaaccgt ctgggcggcg
     9301 tgcaccagtt tttgtacgaa cgataacagc tttaaccaca tcaccttttt taactgctcc
     9361 accaggagta gcttgtttta cagaagcaac gattacgtca ccgatgttag cgaatttacg
     9421 tcctgaacca ccaagtactt tgatagtcaa gatctcacga gcaccgctat tatcagcaac
     9481 tttcaagcga gtttcttgtt gaatcatttc aattttctcc ttttagtttg attagataat
     9541 aacagctttt tccactactt ccacaagacg gaaacgtttt gtagctgaaa gtgggcgagt
     9601 ttccatgata cgaacgatat cgccttcttt agcaacgttg ttttcatcat gtgctttata
     9661 ttttttagaa tagttgatac gtttaccata gactgggtgg ttacgtttag tttcaactac
     9721 aactgtgatt gttttgtcca tcttgtcaga cactacgcgt ccgtaaagag ttttacgttg
     9781 attacgttcc atttagaatt tctcctttcc caatctatta tttcatttct gattgcacag
     9841 tcttaacacg tgcaatttgt ttcttaactt cgtcaaggcg agcagtcttt tcaagttgac
     9901 ctgctgcagc ttggaaacga agatcaaaca attctttttt gagttcgttt tctttcttag
     9961 caagttcttc ttgagacaat ccacgaagct ctttaacaaa atctttaatt tcttgaagtt
    10021 tcatgtcttc tccttattct gcttcacgtt ttacgaattt gcatttaact ggtaatttgt
    10081 ggctagcaag acgtaatgct tcacgtgcaa cttcttcaga aacaccagca atttcaaaca
    10141 tcactttacc acgtttaact ggtgctaccc aaccttcagg tgtcccttta ccagaaccca
    10201 tacgtacacc gatagctttt gcagtgtatg atttgtgtgg gaagatttta atccaaactt
    10261 taccaccacg tttcatgtaa cgagtcatcg caatacgggc agcttcgatt tgacggtttg
    10321 taatccagtg gctagttgta gcttgaagtc cgtattcacc gaatgaaact tctttaccac
    10381 ctttagcctc accgcgcatt tttccacgga attcgcgacg gtgtttaaca cgtttaggta
    10441 ctaacatttg ttatttgcct cctttagtgt ttttacgagc tggaagaact tctccacggt
    10501 aaatccaaac tttaacgcca agtttaccat aagttgtgtc agcttcttcc caagcatagt
    10561 cgatatcagc acgaagcgtg tgaagtggaa cagttccttc tgaataacct tcagcacgag
    10621 cgatatcagc accgttcaaa cgaccagaaa cttgagtttt aataccttta gctcctgcac
    10681 gcattgtacg ttggattgct tgtttttgag cacgacggaa agcaacacgt tgctctaatt
    10741 gacgagcaat attttcacca acaaggtgag catctaaatc aggttgtttg atttcaatga
    10801 tgttgatgtg tacttgtttt ccagttaatt tgttaagttg accacgaaga gcgtcaacgt
    10861 ttgctccacc tttaccgata accatacctg gtttagcagt gtgaagtgaa acaataactt
    10921 tatttactgc acgttcaatt tcaatagttg aaactgatgc gtcagctaat tctttattaa
    10981 tgaatttacg gattgcaaga tcttcatgaa ggtaatccgc gtattctttt tcagcatacc
    11041 atttcgcatc ccagtcacgg atgattccga cacgcatacc aattggatgt actttttgac
    11101 ccacgatttt acctccttat ttttctgata caactacagt tacgtgagtt gtacgtttgt
    11161 tgattggtga agctgaacct ttcgcacgtg gacggaaacg tttcattgtt ggtccttcgt
    11221 tagcgaaagt ttcagatact accaagtttg ctttttccaa accaaagttg ttttctgcat
    11281 ttgcaattgc tgagttaaga gttttctcaa taacacgagc tgctttgttt ggagtgaatt
    11341 ttaagattgc gattgcgtca gcaacattct taccacggat aagatcaagt actaaacgtg
    11401 ttttacgagg tgaaacacgc actgtacgag ccattgcttt agctgaagta atttctgcca
    11461 ttgtgtcctc ctcctattaa cgacgtgttt tcttgtcgtc agctgcgtga cctttgtaag
    11521 tacgtgttgg tgcaaattca ccaagtttgt gacctaccat gtcctcttgg atataaacag
    11581 gaacatgttt acgtccatca taaactgcga ttgtgtatcc gatgaaactt gggaaaatcg
    11641 ttgaacgacg tgaccaagtt ttgattactt ttttcttttc gtcatttgct tgagcttcaa
    11701 cttttttcat taaatgctcg tcgacgaaag gtcctttttt aagactacgt cccattttgt
    11761 agttttctcc tttaaataat gtaccacaac ggcctgtaca ctatagtgta ctaccgagct
    11821 ggcggatttg tataagatta tgctattaac ctattatttt tggttacgac gacgaacaat
    11881 aagtttgtct gatttagctt tcttattacg agttttaagt ccaagcgcag gtttacccca
    11941 tggagtagat ggtgctttac gaccaactgg tgctttacct tcaccaccac cgtgtgggtg
    12001 atcgttaggg ttcattacag aaccacgaac tgtagggcga acacctttcc aacgattacg
    12061 tcctgcttta ccaatgttaa caagtgattg ctgttcatta ccaacagtac caattgttgc
    12121 actgcaagta ccaaggatca tacgtacttc acctgattga aggcgaacaa gaacatattt
    12181 accttcttga ccaagtactt gagcagaagc accagcggca cgaatcaatt cagcaccttt
    12241 acctggttgc aattcaatat tatggataac tgtaccgact ggaatgttag caagcggaag
    12301 tgcgttacca actttgatgt ctgcttctgg accagaaata atgcgttgac ctacttcaag
    12361 gcctttaggc gcaaggatgt aagctttaac accatcagta tagtgtacaa gagcaatatt
    12421 tgcagtacga tttggatcgt attcaattgt tttaacaact gcttcaacac catctttatt
    12481 acgtttgaag tcaatcaaac ggtaatgacg tttgtgtcca ccaccttggt gacgaacagt
    12541 aatacgtccg ttgttgttac gtcctgcttt attctttagt gaaacaagca atgatttctc
    12601 aggagtgttt gtagtgattt ctgcaaaatc caaagaagtc atattacgac ggccatttgt
    12661 cgttggttta taaactttaa tacccacgtt atttcctcct tagattattc agcttcagca
    12721 gcaaacaact cgattgcttt tgaatcagct gtaagagtga taatagcttt tttagttttt
    12781 gaagtaaaac ctgtgtaacg accaacacgt tttgctttgg gtttaactgt cacagtgttt
    12841 acacttgcaa cttttactcc gtcaaatgca gcttcaacag cttgtttgat caaaagtttg
    12901 tgtgcacgtg tgtcaacttc aaatgtatat ttccctgctt caagagcaac cattgatttc
    12961 tcagtgataa caggtttttt gattacgtca tacaaattca ttatgcaaga accccctcga
    13021 ttgtagagat tgcttcttta gtaacaagaa gtttgtctgc gttaacgata tcaagaacac
    13081 ttgcagtagt tgcagtagca actgtaacgt ttggaaggtt acgcgctgaa agtgcagcaa
    13141 attcgttacc ttcttcaagg attacaagta cttttgaatc aatgctaagt gctgaaagaa
    13201 ctgacgcaaa ttcagcagtt tttggagctg caaatgaaag gttttctaca gctacaaatt
    13261 tatcttcagc aacttttgct gaataaactg atttcaacgc aaggcgacga actttttgtg
    13321 gaagtttgta gccgtatgaa cgtggagttg gtccaaagac aacaccacca ccacgccatt
    13381 gtggtgagcg gatagaacct tgacgagcgc gtccagttcc tttttgacgc catggtttac
    13441 gtccaccacc agatactgct gaacggtttt taaccgcgtg agtaccttgg cgaaggctag
    13501 cgcgttggct gattacaaca tcaaaaacaa ctgattcgtt tggttcgata ccgaagatag
    13561 cttcgttaag ctcaactgag ctaacttctt taccagtttg gtcaaatagt ttaacgtttg
    13621 ccattttaac tgttttctcc tttcttatta tttagcagct ttaactgctg atttgatagt
    13681 gataagagat ttcttagcac ctggtacgtt acctttgatg aggacaacgt ttttctctgg
    13741 gataacttgt acaatttcaa ggttttgaac tgttacacgg ttaccaccca tacgtcctgc
    13801 caagcgtttg tttttgaaaa cgcggttagg cgcaacaggt cccatagaac ctggacggcg
    13861 atggtaacga gaaccgtgag ccataggacc acgtgattga ccatggcgtt tgataacacc
    13921 ttggaaacct ttaccttttg atgtccctgt gacgtcaaca acatcaccag cttcaaattg
    13981 ttctacagat aattctgcac caacttctaa gccttcaatg tttttgaatt cacgaatgaa
    14041 gcgcttagga gctgtgtttg cttttgcaac atggcctttg gcaggtttgt tactcaatac
    14101 ttcacgtttg tcatcaaaac caacctgaac tgcttcgtaa ccgtctgttt caacagtttt
    14161 aacttgaagc acaacgtttg gagttgcttc aatgacagta acagggatga attcgcctga
    14221 ttcagtgaag atttgagtca ttcccacttt tttccctaag attccttttg tcatgagaaa
    14281 atatttcctt ttcttatttt atttcaaaaa gttttttacg agcgtttttt gtgctcagaa
    14341 atcaaattaa agtttgattt ctacgttgac accacttgga agatcaagtt tcatcaaagc
    14401 atcaacagtt ttttgtgttg ggttgatgat gtctaccaaa cgtttgtgtg tacgcatttc
    14461 aaactgttcg cgagaatctt tatatttgtg agtcgcacga ataattgtgt aaagactgcg
    14521 ttcagttgga agcggaactg gtccagcaac tgtagcacct gtacgtgttg cagtttcaac
    14581 gattttttct gccgctgtat caagtgtacg gtgttcgtac gctttcaaac ggatacggat
    14641 ttttttgttt gccatctttt ctcctctcgt ctatttacga taataggcta gctcctcaag
    14701 aaaaccaaca cgagttgcgt ggcaatgcaa cctagcgtgt cgcaacctct tgcatcatag
    14761 ctaaagctgt tcatacacag caccagtata tgatatcaaa aaaaaacagc acacgcaagc
    14821 attattttat ttttttttca aaaaaaatgc aaccatttct ggttacattt tttgaatata
    14881 tcgatgatag cgaactttca aaaacagcca cctaaagata ttatctgcta tcgtagccat
    14941 ccaaacccca agcaaaccta attcaaaaac tattcctaat aaataaccaa gaactactct
    15001 gattaaccac atgcctatag tcgttgtgta aaatgggagt ttagcatttc ctaatccttg
    15061 ccaagctgct gtataaacca aggtcccaat agtagcaggt actcctacga aagatagcaa
    15121 aataacaatc aaacttcctt taatggcaga aggattattt gtaaataact gtgtcaatgg
    15181 tttgccggca agaagcataa aacttgatat aaggatcatt agaactaagc caatataata
    15241 cgaactttga atagtttctt ctatagattc tctatttttt tgtcctacat attttgcagt
    15301 taggataatt gtagcagttg cgatgccaag tcctggcata taattaaatt gtgtcaaggt
    15361 ttctccaata gcatttcccg ctacaacatt tgtacctaac tgaacaacaa tagcaactat
    15421 aacaacatct ccagctcgca tcatcagacg ttctcctgct gatggtaagg caagattcca
    15481 aatttgtgca ctgattttcc aagtcattct ttttattatt tttttaatag gaagatactt
    15541 cgcaaggata caaataccaa ttaatcttgc gataagagtt gatactgcca ctccgaccaa
    15601 accatagcgc cactcaaaaa tagcaaaacc tgataagact gcattgagga aattgacaaa
    15661 aatactgaca tacattggta aacgaggttg cccttgcaca cgtaaaaatg agcctagggt
    15721 ggttaatatt cctaaagtaa caacacctcc acccacaatt gctaaatata aaccaccaac
    15781 ttgtgcgaca ctttttgttg tccctaaaag tttaaagaaa gtttgtccaa aaacaatcga
    15841 gataattcct aatactgccc ctatcagtga tgttagaaaa atggcttgag aacatactga
    15901 aattgcatca tccttctcac tacctgctaa cgacttggcc aatagacttg ctatactcgc
    15961 ccctaaagct ataaaaatag cttgataaat agtaattata ttattagcaa ctgaaacacc
    16021 tgatactgct acaacaccta actgagccac tagataatta tcaactactc ccatgagcat
    16081 ttgtaagata ttttcaatca tagctggtat agctaactga ataatctctt tactataacg
    16141 cacagtatct cctttctatt acccaggatc attaaaaaga gagtgatggg aaaatttcta
    16201 aaatgtttct ttctcgctct aaatatttta aaacaatcgt atttttatta ttgtttcaaa
    16261 cctagacact cttcaaccgt ttctcgcata tttgaggtag gtactaccat tctatggatt
    16321 acttcggcct tttgtagatc tgtaaccgcc ttaggtaaag aaacacctga caaatcgttt
    16381 aagattgaaa tagcagcgaa atcctgtatc tcttctttat ttgtaatagc ctttgtaaca
    16441 acacacggga acttataagg acttgctgtt gacacaatta ctgttggagt ttgatcttta
    16501 gtttctgtct gataagcttg ataaacagct gatgctacag ctgtatgcgg atcctctata
    16561 tactgatact ggtcaaataa acgtttgatg tctaattcaa caaattgttc tgtcgcaaat
    16621 cccgctacaa agctctctaa aatatttgct tgcctagcct ctaaggcata ctccccagta
    16681 gtcactaaat cttccattaa ttttttagtc gtttctgcat catcgcctaa aaggtgaaag
    16741 attaatcgtt ctagattaga ggagaccaaa atatccatag agggacttga tgtcacttta
    16801 aattctcgat ttttatcgta ggtttgtgtc ttaaaaaaat ctgttagaac gttattatca
    16861 tttgaggcac aaatgagttt ggtaacaggt aatccaattt gactagcata atatgctgct
    16921 aaaatattac caaaatttcc tgtaggtaca ctaaaattaa ttagttgacc aattgagatt
    16981 tcctttgact taactaattg agcatatgca tatatatagt aaacaatttg aggaacaagg
    17041 cgaccgatgt tcattgaatt ggctgatgat aactgcatat gctgctgtga taattttgat
    17101 cgaagaaggg aattattgaa catttcttta accgaagtct gtgcatcatc aaaatttcct
    17161 tcaatcgcaa caacatgggt attttgcccg gcttgtgtta tcatctgcaa ttcttgaata
    17221 taactaacac catgtttagg ataaaataca ataatctcag ttcctggaac atctgcaaaa
    17281 cctgccatcg ctgccttacc agtgtcacct gatgttgctg ttaaaataac gattttatca
    17341 tcaacccctt gttttttggc tgctgttgtt agcaaataag gaagaatgga taaagccata
    17401 tctttaaatg caattgtagg accgtgaaat aattcaaggt gataccggtc tccaatcttt
    17461 actattgggg ctatttcagt tgtatcaaat ttagtatcat aagcttgact aatacagtaa
    17521 tccaattctt gttcagtaaa atcatcaaaa aaagctgaca aaacgagttt agccacttct
    17581 tggtaggaag catccttaag tttagtgaaa tctaaatcaa cttttggaaa tgtgatgggg
    17641 gtaaagagtc ctccatcgtc agctaatccc tttagaatcg cttgactagc tgtcacttta
    17701 ttactagcat cacgtgttga ttgatatact attgccataa gaatccctct ttatataaat
    17761 atcttttagt tattgtaaca caatttaata acatgtccat tgttcgttat ataattaacc
    17821 cccctttatt aaaataaagg ggggcgtaat atcattatca ctttgtaaag tcgagaactt
    17881 tacgtccttg aatcaatcct ctttccattt catcgaatac ttgaggagct gtatccacag
    17941 gcactttttc aaccactggt actaccaaac cttctgctcc aaatgcgaag gcttcttcta
    18001 aatctttacg agttccaact aaggaaccaa ccacacgaat cccatcaagt actgttttta
    18061 caatactcaa ttccatatat tctgatggaa gaccaacagc taccactgta cctccagcac
    18121 gaacgctgtc aatcgcctga ttaaacgcaa ctttagaaac agcagtcaca accactccat
    18181 gacaaccgcc tgtcttttct tgaatatagg ccgcaacatc ttttatttct ttgccattaa
    18241 ctgtcaaatc tgctccaacc tctttagcta attgaagttt atctgcgtta atatcaacag
    18301 ctacaacatg agcattgaat actttttttg catattggac tgctaagttt ccaagaccac
    18361 ctgcaccata cactgcaatc cactgaccag gagcagctcc agcttctttg atagccttgt
    18421 atgttgttac tccagcacaa gtgattgatg atgcttgagc tggatctaat ccctcaggaa
    18481 ccttaaccgc atagtccgcg gtaacgatag cgtattcact cataccacca tcaacactgt
    18541 atccagcatt tttaacacta cgacaaagcg tctcacgtcc tgtagtacag tattcgcaat
    18601 gaccgcaccc ttcaaagaac catgcaatag agacacgatc accaaccctc aaagacgtta
    18661 cgccttctcc aatttcttct actataccaa tgccttcgtg acccaaaatg cgtcctggta
    18721 cctggccgaa atctccatgt gcaacgtgta aatcagtatg gcacacacca cagtattcta
    18781 cttttacaag tgcttcacca tgaccaactt ttggaagatc atgctcaaca acttcaacac
    18841 ctgtactagc ttgattaaca acaaccgctt tcatgataaa acctcctaga aataaatata
    18901 tcttatttgt aatcgtttac atttttatat tgtattatta ttcacaaact atgtcaagtg
    18961 attttcagaa aattttttga aaatattatt aactggttaa tctttttgaa actaaaaatc
    19021 ccactcaata gagtgagatt cttcgactgt tgttacttac ggcgtcctgg acgttcctca
    19081 taaccataat aagcatcttc gatgatttct tgcatgtgat ctaccattgg aaggcgtgga
    19141 tttgctggtg aacattgatc ttcatatgct aagaaagcta gttcacgaga tttttctttc
    19201 cattcttttt catcaatgcc ttggtcacgg aaattcatct taatacctaa acgtgttcca
    19261 aggtcataca cagcttttgc atatgattcg acagcttctt ctggtgttgc tgctggtaag
    19321 ccgagtaatt tagcgatatc ttgatacttt tcatcagcac gatagtaatt gtacttaggc
    19381 catgttgctg ttttagctgg acgagttcca ttatagcgga taacatacgg caaaagaatt
    19441 gcatttgtac gtccgtgtac ggtatggaat tgtgcgccaa ttttatgcgc cattgagtga
    19501 gaaataccta agaatgcatt ggcgaaagcc atacctgcca tagttgacgc attgtgcatc
    19561 ttttcacgtg cttcaaaatc agcatctttt actgagcgtt ctaagtagtc aaatacaatt
    19621 ttaattgctt gaagagcaag tccgtctgta taatcatttg ccatctgtga aacataagcc
    19681 tctgttgcat gtgtcaatac atccataccc gtatcagccg caataaaacc aggaacagtc
    19741 ataacaaggg ccggatcaac gatagctaca gttggtgtta atgaataatc agcgattgga
    19801 tatttacgat tatttgcttt gtcagagata acggcaaatg gtgtcacttc agaacctgtt
    19861 ccagaagttg ttggaatagc aacaaatttt gttttcttac caagttctgg gaatttgaac
    19921 gcacgtttac ggatatccat gaatttttga acgaggtcat ggaaatcaac ttctggttgt
    19981 tcatagaaga gccacataac tttcgcagca tccataggag aaccaccacc taatgcaata
    20041 atagtgtctg gtttgaaggt acgcattaaa tcagtccctt tcattactgt tgtaatgtct
    20101 ggatctggtt ctacttcagc aaaaatctga taaacaactt tattgcgacg aagatcaagt
    20161 tgttcaatga tacgatccaa gaaaccaagc tcaaccatgg cgtgatctgt aacaatcatg
    20221 acacgttcaa catcgcgaca tttttgcaaa tattgaatag aatcacgttc gaagtatgtt
    20281 tttgaaggga ctttaaacca ttgcatatta tttctacgtc ttcctacttt tttgatattc
    20341 aagagattta tagcactaac gttatcacca actgagttac gtccatacga accacaacca
    20401 agagtcaatg atggcaagaa agcattgtaa acatccccaa taccaccaaa agtagaagga
    20461 gaattccaaa taacacggat agcacgaatt ctagttccaa attcacgagc taagtctgca
    20521 tcttttgtat gaatagcagc tgagtgccct agaccattaa actcaaccat ttgacgagct
    20581 ttttcaacac catcttctgt agattcagct ttcaatactg caataacagg tgatagtttt
    20641 tcacgtgtca aaggttcttt ttcactaact tcagtacatt ctgcggcaag aatatttgtt
    20701 ccttctggaa cagtaaatcc agcttgttca gcaatccaaa ctgctgattt tccaacaata
    20761 tttggattta attttgcacc agcacagttt ttgctatttg cttttgcacc aaaacagaat
    20821 tcttcaagaa gagctttttc tttcttatta acgaaatagg tatgataaga tttaaattct
    20881 tcaacaaatt ctttataaat ttctttatca ataataacag cctgttctga cgcacatacc
    20941 ataccgttat caaatgattt agacataaca atatcgtgag cagcttgacg gatattagct
    21001 gatttttcaa cataagctgg aacattccca gcacctacac caagggcagg tttcccacat
    21061 gagtaggcag ctttaaccat cgcattacca cctgtcgcaa ggatagtggc aataccatca
    21121 tgtttcatca aagcattagt tgcatcaatc gatggttgtt caatccattg gacacaattt
    21181 tcaggagctc cagcagcgat agcagcatca cgaacaatac gtgctgcatg tgctgatgat
    21241 tcttgtgctg atgggtggaa ggcaaaaata attggattac gtgttttaag agagattaat
    21301 gacttgaaaa tagcagttga agtcgggttg gttgttggcg taataccaca gacaactccc
    21361 actggttcag caattaatgt caagcctgtg acatcgtctt cttcaataac tcccactgtc
    21421 tttgtatgac gcatgttgtt aacgacgtgt tcacatgcaa ataaattttt tgtcgcttta
    21481 tcttcaaaga caccacgacc agtttcttct actgcatgaa gagcaagttc tccatgggca
    21541 tccaaggctg caacagaagc tttagctaca atataatcaa cttgttcttg attaagttga
    21601 cgcatctcat caagtgcttt taaaccgttt tgaactaatg tatcaattgc aagagcaaca
    21661 tctgtagttt ctaccgcttt agttttttca gtcatttgag actccttttg atttgttaaa
    21721 tgtttcacaa atttgtttac gagattatta tagcatattt aaaaactttg taaatagttt
    21781 cacaactttt ttgtgaactt tttttcaatc tttcggaaac atttcttgaa agcgctttaa
    21841 ttaaatttgt tttttcatta ctaattttca gattaaagcc aatcgatttc atttgtatca
    21901 ccataaacgg aggtatctat tattcgtttt cacaaaaaag ccgagtacat gactcggact
    21961 tgtttatttt gcagctcgtt gagctgattt ttgaatagct tttttgacgt tactaacata
    22021 taaatcagcc cctgtagtat cgctatcact ataatgcaca ccatctgtat ttccccagat
    22081 ttctggatgt tctgtcgcaa ccttatacca gtcagcaacc gttatgtaat tatatttttt
    22141 cattagcgag agttcataat cacgcactgt cgtaacttga ctcatatttt tagcattata
    22201 aggcgttact ataatcaaac ggtgtccctt aggcaaagat ttaataaatt ggctcaagtc
    22261 tgtcttgtag ttatctaatg aattcacacc aactgccaaa acaacaattt tggacaaggc
    22321 tttattttga atacggtttt caaaaatatc aaatgattta ctaaaattac gactcaccgc
    22381 agcatctaat tgtacttctg gtaacaattt attaaaagca gcacttgacc ttaaggcaac
    22441 tgaatctccg attaccgtca catcactaag agcacctgca tcccctgcgg ctagtgtatg
    22501 tgttcggtta gtatttgttc tagcttgttg taacgaattc tgcaataatt ctgtctcaaa
    22561 acttcctata cttggagctg tcaacattgt cactacagta ataagagtta gtacccctcc
    22621 gatactaaat aaccactttt tgtaaggcaa caagtcaaat tcataatcaa ggaatttagg
    22681 ttttctacct aaaataaaag gttcaatgat ataaaatgat aatgttgaga agactgctga
    22741 taatactact gtcaaaataa cagctatcca gtttggggat aaacgactaa aaataatata
    22801 aaatggccaa tgaaaaaggt acatcccgta actgatatca gcaagataag taatcacgaa
    22861 aggttctgaa atatctggtg tatgctcgtg taaaatacgt gcattgtaaa tcattacact
    22921 agcaaaaatg ctagacaaaa caaaaccaaa aagatatgtt agccgattat caaaatcaag
    22981 tgcaaaagtt aaaatcatta gaccagcaaa agcactaccc atcatgatta aattatgctt
    23041 caaagtcaga tttttgatat ttttcttgaa tcgtccggta atctctcgaa taccagatat
    23101 tgtagccacc atcgctccta aaaagaacgg aaatatatgt gataatgtag agaaatatat
    23161 cgttgaaaaa ttatcaacga aaaaggctct aacaaacatg gtaaggaagc tgactccaaa
    23221 aactcccatg gaaacaagga acaaagtccc cctcaattgc ttttgatctt ttgatctttt
    23281 cgataagagc cataccgtta agccccataa aacataaaaa tggacttcaa tagaaagact
    23341 ccaagtatgc acaaataagt gagggataaa ttgactttcg tagttaccac cagtcaagat
    23401 ttcgtaaaag ttgctggtaa aacctaaagc agtcataatt tgagagccaa tacttgctct
    23461 aaaatcgctt ttaaccagaa atacaaaagg tattgtcacc aaaaccatca atactaatgg
    23521 tgggaaaata cggtaaaaac gtctacgaca aaaactaaca aaatcaattt ttttcgtttt
    23581 agaaaattca tcaatcaaca aggccgtaat cagaaaacca gaaaatgtaa agaagatgtc
    23641 aacaccaaca aatcctcctg gaaaactatt cttaaagaaa tggtaaagta aaacaagtag
    23701 tagtcctgtt atacgtacca aagaaaacca ttttattctc atttttgata aatcccctgt
    23761 ttaaatgtcc ccttatagac tttcttattt ttagcattga ggcgaccttg tccatcaggt
    23821 tgaccttttt taaagtcacc tgtataagac cagccatgaa ctgaaacaaa tgttcctttc
    23881 ccttcaaaga ccccatttac aaaatcgccc ttatagtaat ccccattctc ataggttaat
    23941 ttacctttac cagtcatatg gtgattaaca atagagccca catattttat cttaccgcca
    24001 tcatagctaa ggactccttg actagaagtc gttaaggtaa atactgataa accaaaaaca
    24061 acaataatta tcaaagataa tatttctaga tgagttcgtg ttattttaaa attcttcatt
    24121 tctacttaat ctctactatt atttattagc gttaaccact gtttacagcc atcactgact
    24181 agacgatagg tttcctcaaa atcaccagta taccatggat caggaacacc accatcacaa
    24241 aaaaggctaa tcttatcatc ataggttcct tgtgatagag cttttaaatc cttgacattt
    24301 tgctcatcca taccgatgat ataatcaaaa tatgctaaat cgtctttaga aataggttgt
    24361 gaccctttat cgttagtgta agtaatttga taacgctgca aaatcgcttg cgttccccta
    24421 tgtataggat ttccatgctc ccatgttgaa gtcgcgcgac tctcaaccga gaggctatcc
    24481 tccttatcta aattcgccat aacaaactca gccatagggc tacggcagat atttccaaga
    24541 caaacaaaac agactttctt catagcaaac tccttttggt ctattataac agaaaaaagg
    24601 tacttataat acgtttaact gctcaaagaa taactacaac atatctaata cttaacattg
    24661 catgaaaatt aaaaaattaa gactagatac cctagcctta aaatgactta tgttttattt
    24721 atcaaatcgc tgataaccta aatgttcgta tgccttatcc gtagctacac gaccggtacg
    24781 ggtacgcata ataaaacctt tttgaattaa ataaggttcg tacatgtctt caacagtatc
    24841 tcgttcttca gtaatattaa cggatagagt tcctaaacca acaggacctc cattatacat
    24901 ttcaatcatg gttcttaaga ttttttgatc gacgtaatca agcccctcgt gatcaacatc
    24961 taacatcgtt aatgctttat ctgtaatatt gtcatctatc aaaccatctc ccatgatttg
    25021 agcataatct cgaacacgtt tcaataaacg gttagcgata cgtggcgttc cacgactgcg
    25081 acgcgctaat tcagaagcag cttcataagt aattttcatt tcaaaaatat ctgctgtacg
    25141 ctcaataatt tctgtcaaat cgttttcttc ataatactcc atatgccctg taataccaaa
    25201 gcgagcacgt aagggattag ataacatacc tgcacgtgtc gttgcaccaa ttaaggtaaa
    25261 gggcggcaaa tctaaatgaa cacttctact agtttctcct gcaccgatca taatgtcaat
    25321 atagaagtct tccattgcac tataaagtac ctcttcaacc gccatcggca tacgatgaat
    25381 ttcatcaata aaaagaacat cacctggttc taaatcattt aaaatggcta ctaaatcccc
    25441 tgatttttca attgcgggac ctgatgtttg tttgagattg acacccaact cattagcaat
    25501 tacaaatgcc atggttgttt tccctaaacc aggagggcca aataataaca catgatccaa
    25561 tgactcatca cgcaatttag cagcttcaat aaatattttt agctgatctt taaccttatc
    25621 ttgtccaata tactctctta aatactgcgg acgaagtgta cgttctacca attcttcgtc
    25681 acccattgca tcactatcta aaaatcttgt catgatatta ttataacatt tgtataggaa
    25741 aagttaagaa ctaagttggt gtattagtat ctcccttatt taaaggaaga ataaactgat
    25801 cttatagtta atccaacctc aagaaatcac agaacagctt acttagccca tattccacca
    25861 acccattcct aaaaacaaat caaatgaacc taaatttgat aatagaacaa gtaatactat
    25921 taaaaaacat tttttaatca ctataataaa ctcctaaata atattttgta cgtttattat
    25981 aaatttttag acctcagagt cacaggacat caatgtcctg ttatatcttc ttgccactcc
    26041 aattctaatt ttttaattag atgcttgtca tcaatcattt ttgaaaacat aattgattgt
    26101 tggtaagcat tttctgcaga ctttatatca ttgtcaattg taatgaagta tttccattgg
    26161 tacataaaat ataaaggcat tttatgaaaa tcatgtgttg acaccatcgt ttgtcggcta
    26221 acttctaaca tttctgcaag acactcttca ctatttaaac aaagtccaac aaatacacaa
    26281 ttcaggagta catgattaaa ccaaaacaaa tcttctgctg taagtttgtg gttctgactt
    26341 atcaaagttt tacatatagt ttgaaatact tctttagtaa atgctctatt gttaaattta
    26401 gatacaactg cacaagttaa aaa
//
LOCUS       DATRNX010000016        25659 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRNX010000016 DATRNX010000000
VERSION     DATRNX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25659
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(44..1411)
                     /locus_tag="VBC81_000549"
     CDS             complement(44..1411)
                     /locus_tag="VBC81_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6911611.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIAGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(1558..2604)
                     /locus_tag="VBC81_000550"
     CDS             complement(1558..2604)
                     /locus_tag="VBC81_000550"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6911612.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(2745..3341)
                     /gene="recR"
                     /locus_tag="VBC81_000551"
     CDS             complement(2745..3341)
                     /gene="recR"
                     /locus_tag="VBC81_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6911613.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(3356..5401)
                     /locus_tag="VBC81_000552"
     CDS             complement(3356..5401)
                     /locus_tag="VBC81_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6911614.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(5530..6222)
                     /locus_tag="VBC81_000553"
     CDS             complement(5530..6222)
                     /locus_tag="VBC81_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6911615.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(6399..7157)
                     /locus_tag="VBC81_000554"
     CDS             complement(6399..7157)
                     /locus_tag="VBC81_000554"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6911616.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(7338..8534)
                     /gene="tuf"
                     /locus_tag="VBC81_000555"
     CDS             complement(7338..8534)
                     /gene="tuf"
                     /locus_tag="VBC81_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6911617.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(8886..10154)
                     /locus_tag="VBC81_000556"
     CDS             complement(8886..10154)
                     /locus_tag="VBC81_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6911618.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(10260..13055)
                     /gene="ppc"
                     /locus_tag="VBC81_000557"
     CDS             complement(10260..13055)
                     /gene="ppc"
                     /locus_tag="VBC81_000557"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6911619.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            13264..15063
                     /gene="pepF"
                     /locus_tag="VBC81_000558"
     CDS             13264..15063
                     /gene="pepF"
                     /locus_tag="VBC81_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6911620.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(15122..15511)
                     /locus_tag="VBC81_000559"
     CDS             complement(15122..15511)
                     /locus_tag="VBC81_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6911621.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(15495..16019)
                     /locus_tag="VBC81_000560"
     CDS             complement(15495..16019)
                     /locus_tag="VBC81_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6911622.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            16176..17024
                     /locus_tag="VBC81_000561"
     CDS             16176..17024
                     /locus_tag="VBC81_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6911623.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(17057..17674)
                     /locus_tag="VBC81_000562"
     CDS             complement(17057..17674)
                     /locus_tag="VBC81_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO6911624.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            17968..18441
                     /locus_tag="VBC81_000563"
     CDS             17968..18441
                     /locus_tag="VBC81_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6911625.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            18463..19104
                     /locus_tag="VBC81_000564"
     CDS             18463..19104
                     /locus_tag="VBC81_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6911626.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(19140..20042)
                     /locus_tag="VBC81_000565"
     CDS             complement(19140..20042)
                     /locus_tag="VBC81_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6911627.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMASERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            20217..21707
                     /gene="lysS"
                     /locus_tag="VBC81_000566"
     CDS             20217..21707
                     /gene="lysS"
                     /locus_tag="VBC81_000566"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6911628.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(21782..22252)
                     /locus_tag="VBC81_000567"
     CDS             complement(21782..22252)
                     /locus_tag="VBC81_000567"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6911629.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(22267..23460)
                     /locus_tag="VBC81_000568"
     CDS             complement(22267..23460)
                     /locus_tag="VBC81_000568"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6911630.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(23478..24128)
                     /locus_tag="VBC81_000569"
     CDS             complement(23478..24128)
                     /locus_tag="VBC81_000569"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6911631.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(24109..25218)
                     /gene="ribD"
                     /locus_tag="VBC81_000570"
     CDS             complement(24109..25218)
                     /gene="ribD"
                     /locus_tag="VBC81_000570"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6911632.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(25412..25526)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         8171 a         5032 c         4047 g         8409 t
ORIGIN      
        1 aaaaacaaaa aagttagggt aacttgttac cctaacccct ctattatcct acttcaagaa
       61 tatcaactat tttagaaaga ttcattgagt tagaaccttt taataatatt tgatctttct
      121 ccttaagagt atcttttact tttgcaagca attgatcaaa ctgatccact tcttgattct
      181 ttttaaagaa atacacttta ccaattggga acatctggct tgctaattga gctaacccct
      241 caatatcctg gccataacaa atcaaagtat caatagagtc aggtctgata ctcataatca
      301 tttgattatg taaatccaca gattgttctc ccagttcttt catatcagct aaaagagcaa
      361 tcttctttcc accatcatta ttgggaatag ctgaaaaggt ttctagtatc aatcgcatag
      421 ccgttgggtt agcattgtaa acatctgata aaatatctgc tccattggct gatttcttcc
      481 attcggtgcg attacgagtt agttgaagat tttctagagc atccactata tcttcttctg
      541 ctactgctaa taattttcca acataggcag cgaccatcgc attagttgca ttgtacttac
      601 caggtactgg tatacgtaat tggtgctcca aagcattagt tttaaaagtt aaggaatgtt
      661 tttcttcttt gagttctgtc acttgcaatt cttggtcatg gccaaaacga atagtcatct
      721 ggtttgctgg aagatatgga tcaataattg gatctcccgg tgcaattaat atgccatctg
      781 aactcatacc atctgtgatt tgcattttcc cttctgcaat cttttctcga ctaccaaaaa
      841 attctaagtg tgcttcacca atcaaagtaa caacagcaat acgtggtttt gcaatttcag
      901 ataacacatg gatatctcca agatgatcct gtcccatctc caaaacaatt ttttcagtat
      961 cttctggcat gtgtagcaca gtatatggta agccaatctc gttattgtag tttccttgtg
     1021 ttttataagt cttatatgtg gttgataaga ttgcagctat catatcttta gttgttgtct
     1081 taccatttga gccagttact gcaataacat caacgttcat cttttcaata taatactgtg
     1141 ccaagacttg aaatgcttta agtgcatcag acactaaaag ataaggatga cccgctattt
     1201 ccttctctga aatggttgct attgctccat tatcaaaagc catctctata aactcatgtc
     1261 catctcttgc tccttttaag ggaagaaata agtcaccttc actgatatta cgactatcaa
     1321 attcaatatt tcccagggga acgtcttcaa actctgatac ttgattttta gcgccaacta
     1381 ctttagcaac ttcatgcaaa ctaagcttca tatcatttcc ttttctatta tttagcaaca
     1441 atgttgtaga tagtatgcta tcccttataa acgaatatac tcctaaatta taacacactt
     1501 tcataacata tcatagattt gaaaaagctc aactaagaaa gacttagttg aactttttta
     1561 aattaaatga ctctcacgtt tttcaaacat ttctttagct aacataacta acttttcaat
     1621 caaatcacta taagttagcc ccatattttc ccaaagcaga ggatacattg accactgagt
     1681 aaaaccgggc attgtattca gttcgtttaa gaagatttgt ccatctttcg tcaaaaagaa
     1741 atcacagcgt gataaaccac aagccccgat tgctttaaaa gctttacttg catattgacg
     1801 cattgcttcc atagttgctt catcaacttt agctggaata tccatagtaa ttttattatc
     1861 aatatatttg gcgtcatagt catagaaatc gacgtcttta acaacttcgc caggaaaagt
     1921 tgtcttaaca tcattattgc ctaaaatacc tacttcaatt tcacgagctg tcacgccttg
     1981 ttcaatcaaa atacggctat catacttgag agctaagtca attgcagagc gaagtgagga
     2041 ttcatctgtc gcttttgaaa tacctactga tgaccccata ttagccggtt ttacaaaaat
     2101 tgggaaactt aaagtttcta aagagagttt aatcgcatgt tccaaatcat caccctcaaa
     2161 ataagtttga tatgcaacct gaggtacacc tactgttgca aggacttgtt ttgttgtaat
     2221 tttatccata gccacgcttg aagatagaat attagtccca acataaggca tccttaaaac
     2281 ttctaaaaat ccttggatag aaccatcttc ccccattggt ccatgtaaaa cggggaaaac
     2341 aattgcatta tcatcataga tatcacttgg acgaaccatt ttgtctaaat caacagtttg
     2401 gtttgtcatt aacttttcat ctgaagatgg catttcatca aattcttgtg ttttaataaa
     2461 ttgacctact tgcgtgataa aataagtttt aacaaaaaat ttatcataat taatagcacg
     2521 catgacgctt tctgcagaca gtacagatac ttcacgttcc gcagagcgac caccgtaaag
     2581 caagatcaat gtttcttttg acatatttct tctcttttct atctaatcta agattgacct
     2641 tttaataata ccatatttta acttaacttt caaaaatcaa tccacttaaa gttagtattg
     2701 taaaagaaaa agttaggaga ttcccaacct tttctttata acaattataa ctctgtacga
     2761 ttttcgattg ctcgaagcaa ggttacttca tctgcatatt cgatatctga accaacagct
     2821 aaacctctcg ctaatcgtgt cactttgata ccagctggtt ttaagactcg tgaaatatac
     2881 attgatgtag cttcaccatc agcagtcgca ttggtagcaa caataacttc tgtcacctga
     2941 ccatccatca atcgagtaat taaacttttc aaattaatgt catcagggct aataccattc
     3001 ataggtgaaa ttaagccatg taagacatga tacaaaccat tatactcttg aattttttcc
     3061 atagctgaca catctttact atcttcaaca accaagataa cagactggtc acgcgtctta
     3121 tctgtacaaa ttaagcaagg atcatcatca gtcaaattac cacaaacaga acaataggtt
     3181 aattcccgct tagcagctaa taaattctta gcaaattcat taacatcttc atcactcata
     3241 ccaatggtgt aaaaagctaa acgtgttgct gttttagtac ctatacccgg taattttgaa
     3301 aagctgtcaa tcaatttagc aattggtgtt gggtaaagca ttcaaatgtc ctctcttatt
     3361 gtcctgtgaa ctgtgaaata tattgatcaa tcatatctcg tgcaactaat tgatgggatt
     3421 ttgttgtatc agttgtaaca tgcggataca ttaccgctac tgctatttta cgattagtat
     3481 cgtaggcaat agcgtttaag ttgtaggtag aaactgtttg tccatttata tttttagcaa
     3541 atgtttcagc ggtaccagtt ttaccactaa tggttgtcac attccccctc attgacgtac
     3601 cagttgcata gccacttcca ctattgacaa cgttataaaa accttcttga ataatggcta
     3661 actcttgatc tgagatagct atcttattta gtgttttagg agtgattgaa cgaaccaatt
     3721 gagcgaactt attagaatca ttcccttcat agatatctga aaccacgtga ggtgctaaac
     3781 gattcccatt attcgcaata gttgagatat actgaccaag ttgcattggt gtataggcat
     3841 catactgacc gaacgattcc attagagttg ttcctaagct gtattttcca ggtacataac
     3901 cttctgattc aggtaaatct aatcctgtag aaacccctaa accatattcg ccgtaagttt
     3961 tccttagttt agccattgct tcttgatagc ctcgatcagt taaagcatct cctgtattat
     4021 agtcttgtcc cattaaacga agtgctactt gaaccatata agtattagat gaatactcta
     4081 gcgcttgtgc cgcagatatt ggagtcaaac ctcgtgtaaa ccatgaccgt atattagcaa
     4141 tttcttggtc ataaagcact tcatttcctc taagaacttt attttcccat cctgatgata
     4201 gcgtcgcacc tttcacaaca gatcctggcg tgaaattctt agcaatagtg cctaggctgt
     4261 catcaactaa attcccatga tcatttttca gcccagccat tgctaacact ttaccagttg
     4321 aaggttctat agcaactgca tacatccctt cggaaaaact agcgcgccca ctagataatt
     4381 cactgccata gtattgtttc aagatacttt ctactttgtt ttggtaatta agatcaatcg
     4441 ttaatttaag atttcttcct gacttacctt cttgcgtaat attatctgaa acaactttac
     4501 cttttttatt aacaactact ttgcgaattt gtctgattcc ttgtaagtca tcttcatatt
     4561 gtttttcaag gtaagaagtt cccactcggt cattcagtga atagcctttt ttcaaatatt
     4621 ttttaacttc ttctcttggt aaacctgcct tttctgtaga aatagtacct aatatatccg
     4681 ataaagaagt tcctttttta cgacgatgcc aattacttgt tacagaaatt cctaacagtt
     4741 ccttcttatc agcatccagt ttcttgattt gatcatcact taattcacct gtacttaatt
     4801 tcactgaacc aaacgttggt gtcgcattca tttggttaaa taaggcaacc acttttaact
     4861 catcttcaga atagttaata gcgctcactg gaactgcagc aactgcattt gcatatacgg
     4921 ttgattcggc tagatggtta ccaaatttat cgtaacgttt agaatctggt aaactttcaa
     4981 caactttttt gtagttggct ttatctgcta aataataatc tctctttgct ctatcgcttg
     5041 aagcaacttc ggttaaggta atgtaatgtg ataatttatt cgctaactct ttgatagact
     5101 gagatgaaat attgtttcct ctagaaaacg ccacagtagg acgttcatca ttttcaacta
     5161 agacaactcc cttagcatca aaaatctgac ctctcgcttt tgattctttc actgtataac
     5221 tcgtaagagc tgtcagtttt ttcatgtaaa atgattgttg cccaatctgc atttgctcta
     5281 aacgaagaat tagaacagta aataataaaa caataataaa aaatagaaga tttaatcgtc
     5341 taggtatgct agcattttgc ttttttacag tgagacgata ccttttttta cgattcaaca
     5401 ttaactcctt gctataaaaa ctctgactac tattgtatca taaaaatggt tttttacaga
     5461 cactcaaaat gttataaata ataaaaaatt caagccaact tggcctgaat tttttatata
     5521 aagattagat tatttaccta agtaatattc tgaaacaagg tttaattttt catcaaattc
     5581 gaaaacaagt ggtgggaagt taggaatttc aacgtccatg atttcatcat ctgacaattg
     5641 tttgatatgt tttacaagag cacggattga gttaccgtgt gcaccaacaa acacattttt
     5701 accatcttta agagcaggag caattttatc ttcccagaaa ggaagagcac gctctaaagt
     5761 aacttttagg ttttctgcat ctggaataac agaatcatct agtgaagcat agcgacgatc
     5821 agtatgtgct gaatgttcat catctttagc catatctgga ggcaatacat catatgaacg
     5881 acgccaaata tgaacttgct catcaccaaa ttgttcagct gcttctgctt tattttttcc
     5941 tgtcaatcca ccgtaatgac gttcgttcaa gcgccatgat ttttcaactg gtacccaaag
     6001 ttgatcagct gcttcaaggg caaggttagt tgttttgatg gcacgtttaa gaactgatgt
     6061 aaaagcaagg tcgaactcaa tacctgctgc ttgaattaat ttcccagcat caatagcttg
     6121 ttgtgtacct ttttctgaaa gatctacgtc agcccatcca gtgaaaaggt tagctttatt
     6181 ccactcagat tcaccgtggc gtgcgaatac taattttacc attagaaaat ttctccttta
     6241 attttggcta taccaatatt tactttaact attctacaaa aaatagccat aaaaatctag
     6301 tctgattact agttcaataa tcaaaaatag cttattatta actatctata agaaaaggca
     6361 tatcacctag agtgctatgc ctttagatat catgtgattt atttaacaaa atctaataag
     6421 gcaaggaagc tttctgcttc aagtgaagct ccacctacaa gggcaccgtc aacgtctggg
     6481 caagccatat attctgcaac gttttcaggt ttaactgaac caccgtattg aacgcgaact
     6541 ttgtcagcaa ctgcttgacc gaagtctgct gcaacaacgt cacgaactgc tttacacatg
     6601 ttttgtgcgt cgtcttgagt tgctgattta ccagtaccaa tagcccagat tggttcgtaa
     6661 gcgataacaa gtgatgatac ttgctcttct gacaaacctg caagagctgc tgaaacttga
     6721 gcacctacga attcaaccgc tttaccagct tcgtaagttt caagagactc accgcaacaa
     6781 atgattggag tcaaaccatt agcaaagata gcttttgctt ttttgttgat atcttggtca
     6841 gtttcatgga aataatcacg acgttctgag tgaccgataa caacgtagtc tgtacccatt
     6901 tcagccaata ctttagggct gttttcacca gtgaatgcac ctgaattctc aaaatagcta
     6961 ttttgagcag cgattttcaa ttctgaacct ttagctgctt caagaactgt tgataaagta
     7021 agtgctggag cagcaatccc tgcttcaaca agttctgatg atggtaattt agaagctact
     7081 gcttcgatga atgcttttgc ttcttctgga tttttattca ttttccagtt accagcgata
     7141 aatggtttac gtgacattac acatacctct tcttttttta tttactaatc tatcatacca
     7201 taaattatag ttaattaaaa gtttcacgag tctagtaaaa cgctttaatt atagatacta
     7261 aaaaagagtc cctgaggact cttcacaatt tgagctgtct gactctaact gccgtttggg
     7321 aacttaatta agttaaatta agcttcgatt tctgaaacga tacctgaacc aacagtacgt
     7381 ccaccttcac ggattgagaa agtagtacct tgttctacgg cgattgggtg aatcaattca
     7441 acttcgatag taacgttatc accaggcata accatttctg ttcctgctgg aagttcgatt
     7501 gaacctgtta cgtcagttgt acggaagtag aattgtggac ggtagttgtt gaagaatgga
     7561 gtatgacgtc caccttcttc tttagaaagg atgtaaactt cacctttaaa tttagtgtgt
     7621 gggttgattg aacctggttt agcaagaact tgaccacgtt cgatttcatc acgttgaaca
     7681 ccacgaagaa gaacaccaac gttgtcccct gcaagacctt cgtcaagttg tttacggaac
     7741 atttcaacac cagtaacaac tgctttttgg atatcttctt taataccaac gatttcaact
     7801 tcgtcgttga cacgaacagt accacggtcg atacgtcctg aagcaactgt accacgtcca
     7861 gtgattgaga atacatcttc gactggaaga agtaaaggtt tgtcagtatc acgttctggt
     7921 tctggaatgt actcatcaac agtgctcatc aattccatga tgatgtcttc gtatttttca
     7981 tcgccttcaa gtgctttaag agctgaacct tggataactg gaaggtcatc acctgggaag
     8041 tcgtattctg aaagaaggtc acgaatttcc atttcaacca attcaagcaa ttcttcatca
     8101 tcaacaaggt caactttgtt catgaatacg ataaggtgtt taacaccaac ttgacgtgaa
     8161 agaaggatgt gctcacgagt ttgtggcatt ggtccatcag ttgaagcaac tacaaggata
     8221 gctccgtcca tttgagcagc accagtaatc atgtttttaa cgtagtccgc gtgtcctgga
     8281 gcgtcgatgt gggcatagtg acgtttttca gtttcgtact caacgtgtgc agtgttgatt
     8341 gtgattccgc gttcgcgctc ttctggagca gcatcgatag aagcgtaatc ttttggttgg
     8401 ttaactgaag taggcaagcg acgtgccaat acagttgtga tagctgctgt taaagtagtt
     8461 ttaccgtggt caacgtgtcc gattgtacca atgttaacgt gtggtttact acgatcgtat
     8521 ttttcttttg ccattttagg aaaagcctcc aataaaatat attttataga tagacggtag
     8581 gcaatacagt ctaactttac ttatctattt taacaaaatc ctctaaaaaa gcaagtattt
     8641 tatgcttttt tgcttatttc tttatttcct taaaattcgg gtaagattga ttgaggcgtt
     8701 tgttatctct tatatgtatt ttttagtagt tatcatataa tcatattgtt aaatacatcc
     8761 taatttatag tattagaata ttaatagaca cttcttttct tcaaatatct tctctctatt
     8821 aattagagtt taaatttcaa aaaatacaaa aatcgagata aaaatctcgt tttttgtttg
     8881 gatgattatt taaaggcatc aaggttgatt attttttcat ttttttcttg tggcttatat
     8941 tgctcttctg cttctttcat tatgagttcc tttttctcgt tagcatcaat attaagcacg
     9001 aatccgatag ctacagaaag taccaagagg ctgttccctc cttgggataa gaaaggaaat
     9061 gtcactccag ttgaggggat taacccagaa ataccaccaa tgttaacaaa aacttgcatc
     9121 aatagcattg ctccaatacc aagagcgatc attgagttga aaggatcttt agcctttata
     9181 cctacgtgca taatacgtaa aattaagaag aaaaccaaag ctaaaataaa ccctgcacca
     9241 ataactccta attcctcaat aacaattgaa aagacaaagt ctgttgttgc ttctggtaag
     9301 taccctaatt tttcaataga attcccaagt cctcgaccaa accagccacc gttgctcatt
     9361 gcataatatg aattagccaa ctggtgtcct gaatctgtta aatctttaaa tgggtcaaaa
     9421 aaagcattaa atcgcttagc tacataacca aagacaggga ctttagccat tgtctcaact
     9481 ccaacaactg caatagttcc gataaataaa gttgacccca cgacgattag accaagtaaa
     9541 gctgaaaacc atcgataacc aataccacta atacagtaca taataatgac cgttagaaca
     9601 attatcgaac catttcctaa atcaggttgt gcaattacca aaccaatcat aaataaagaa
     9661 taaaatcgcc aatcttttag gtcgcttagt gatctcggaa gccatctccc ttttgtcaaa
     9721 gcttgataat catatatttc tatctttttt tgccttcgag caaaagtaaa cgctaaatac
     9781 catacaatga taacttttaa atattcagca ggttgaaaac taataggacc aataacaatc
     9841 caaccatgtg ctccattaac ttcttgtgtg aaaaagcgcg ctattagtag caaaaatact
     9901 tctaccaata ctgccatagt caacacttta gaatttttca aaaaatttag tttcagttta
     9961 tagataaaga taattgcaac caaactgact gcccaaaaga caccctggtt gattactgaa
    10021 cgaaaagggt tagctccaag ctgaattaga gttgcactag ttgtcgaata aattacaatt
    10081 aagcctagaa tagacaatat caaataggga atgaggatgg aataattcag aagatgcctt
    10141 ttatcaattt tcatattgta ttatttcctt taatatatca aaaaattacc attatgcatt
    10201 atatcatctt tcgaagtaaa gtaaaaggga gaatagcaat gatcctccct ctatcaatat
    10261 taacctgaat ttcgtaaacc tgttgcaata ccattaatag taatatgaat caacttctca
    10321 tagtcttcat ctagttggtt actacgaaga cgtttaatca attcaatttg aacataattc
    10381 aaaacattga aatatggcaa acgataatct aaacttgcat gtaacattgg gttttcttct
    10441 aataaattat catgctgttc aatagctaaa atcatatctt tagttaactg ccattcatta
    10501 aggatgatat taaaaacatc tcgaacttct ttacttccag ctaactgagc atactgaagc
    10561 gcaatattca tatttgattt tgataaaacc atatctacat tagataaaag agaattaaag
    10621 aatggccatt tttgatacat ggtttgtaat tttgctaagt ttgcctcatc ttgttcaata
    10681 aattgcttga aggctgaccc aacaccatac caacccggaa acataatacg attttgtgac
    10741 catgaaaata cccatggaat tgcacgtaaa cctgaaattt ctgtaattgt tttacgtgct
    10801 gctggccgag aaccaatatt taaacttgat acctctttaa ttggacttgc ttcaaagaaa
    10861 tagtcataga aataaggatt atcaaaaact aaatttctat aaacggcatt actttcagcc
    10921 acaataccat ccattgtctc acggaaatta tcaatttcat taaggttagt aatcatacgt
    10981 gttaccatgc ggtctatgga agctgaaata agcatttcaa ggttataata ggctgcatct
    11041 tgattaccat atttattctc aatgatttca ccttgctctg ttaggcgaat tcgatctttg
    11101 attgaaccaa aaggttgtga tgtgatagct tcataagatg gaccaccacc acgaccaaca
    11161 gttccaccac gaccgtggaa aaatgtgatt ttaataccgt tttcttcacc tatttttgtt
    11221 agctcatttt gagctttata tagagtccaa ccagatgata aatagccacc atctttatta
    11281 ctatctgaat aacctagcat tatttcttga taattgttgt tagttgtaat ccattttttt
    11341 actaatccat aatgtagata ctgggtcata atatcacgtg aattatccaa atcttcgatg
    11401 gtttcaaata acggtacaat ctgaatgcga gcttgattgg catcaattag tccaacttct
    11461 tttaacatga tagctaactc aaacatatcc gaaacgctct ctgtgtgaga aatgatatgc
    11521 tgttttataa tgtcttcacc caattgatct tttaactcac gtgctgtttg gaaaatagcc
    11581 aattcttttt gaagtaactc ggatttagga gtatgcgtag aagataatgt tctaggatct
    11641 tctgttaatt cttttaagag aagttgacat ttttcttctt ctgaaagaga actgtaatca
    11701 tctacaatat tggcagactt caataattct gctacacaag cttcattaat actagaatct
    11761 tgacgcatat caatagtagc taaataaaat ccaaagatat ctactgcttg aataagacaa
    11821 gctaattcgc catcgacaag agaatcttgt ccatgttgaa ctaaagattg cttcacaagt
    11881 aacaaatctt gtttaaactc ctcagcagta gcgtatgctg gtagttcttc tgtctgtatg
    11941 tcagaagcta cttgagcgat atgtgatttt atataccttc ctacaatacc agaagattct
    12001 tggcgactaa caaatttctc ttttgggtaa gaaccaactt ttaaatttag tagggtttgt
    12061 actagtttat cttggataaa attgaaagct ttacgataag gttcattttc cctatatact
    12121 gaattatctt gtgactggtt tgccaacgta acaagttcgg gagagacttc agttagactt
    12181 attgaaagtg acatattacg ataaagttca tttagctgct caatataatg gttaataatc
    12241 acttcacttt gtaccatagc tgataatctt agtgtctcag cagttacaaa gggattgcca
    12301 tctctatcac caccaatcca catccccata gtcagaggct taggattctc taagtgaata
    12361 ccttttttag ctgccagtgc cttatattct gctgttaatt tggtaactgc tttaataaga
    12421 gaacggttgt aatattccat tacattggtt atctcatttt tgactttcaa ttttttctcg
    12481 cgaatagtgt ctgtttgcat gataatgccg atgtaacgac gcaagtcagc ataccatttt
    12541 tcttgattca cgattcctgc tttgacatct ctgtatttac gtaataaatc atgaatctta
    12601 gacgttaatt cgagcaccgt tttacgctgc acctgagtag gatgagctgt tagcacaggc
    12661 acaacattga cgtgttcaag aatatcttta gcattttcat ggccagctac aacatcaata
    12721 gtcgttgata actttcctag gtaatcttgg ttaagattat ttttataatt aatttcatat
    12781 gctaaatcaa cgtcttctga aatattaatt aagagcggca aaatagagaa gtaacgagaa
    12841 acaataacca tctcctcgtt gcttaaagcc gaaatagttt ccttcaaacc agtataatcg
    12901 tcagttgatg ataatgaaac tattttttca atcttgtcaa atgattcact accaatcaat
    12961 ttttgcgttg cttcatccaa aagctgcttc agtaaaccga cctcttcggt aatgatttct
    13021 ttattgctac tactttctaa tttcgggtgg gacaaaccat cacttcctct cttaaatctc
    13081 actactatga taccatttta aagtaaaaag taaaccattc taggattttt agatagaatt
    13141 ttcagatttt tacttattta tgacagtaat cagtttttct tgttcgactt ttttgatata
    13201 ctgaggataa ttgagattaa ctgattaatt gttaattaac aactattatt aaaggagtat
    13261 cgcatggaac ttaaaaaacg ctctgaattc cctgaaaatg agctttggga tctgactgct
    13321 ctctacaaag atcgtcaaga ttttttactg gctattgaaa aagctcttga ggatatcaag
    13381 gttttcaaaa aaaattacga gggaaaactt aattgtgttg aggattttac tagtgccctt
    13441 atggaaatcg agcatattta tatccagatg agtcacattg atacctatgc ttttatgcct
    13501 caaactactg actttagcaa cgaagagttt gcacaaattt ctcaagcagg atcagacttc
    13561 gcaactaaag ctaatgtact actttctttc tttaatactg ccctagcaaa tgcagatata
    13621 aagattttag attctttaga aaataaccct catttcaaag caactattcg ccaagctaaa
    13681 attcaaaagc aacacttatt aagtccagaa gtcgaaaaag ctttgacaaa cttaaatgaa
    13741 gtcctaaata caccatatga tatttatact aaaatgcgtg ctggcgattt tgatatggaa
    13801 gattttgaag ttgacggaaa aacctacaaa aatagttttg taacttatga aaattacttt
    13861 caaaatcacg aaaatgcaga gatacgtgaa aaatccttcc gttcattctc gaaaggtcta
    13921 cgtaagcacc aaaatgctgc agctgcggct tatcttgcaa aagtcaaatc tgaaaagtta
    13981 attgctgata tgcgtggtta cgatagtgtt tttgattatc ttctttccga acaagaagtt
    14041 gaccgttcaa tgtttgacag acaaattgat cttatcatgg atgaatttgg tccagtagct
    14101 caacgattcc tcaaacatat cgccgatgtt aatggtattg aaaagatgac ttttgcagat
    14161 tggaaattag acattgataa cgaacttaat cccgaagtaa gtatcaatga tgcttatgac
    14221 ttagtaatga aatcagttgc tcctttagga aaagaatatt cccaagaggt tgagcgctat
    14281 caaaaagagc gttgggttga ttttgcagct aacgctaata aagattctgg aggatatgct
    14341 gcggatcctt acaaagttca tccctatgta ctcatgagct ggacggggcg tatgagcgat
    14401 gtctacacac ttattcatga gattggacat tctggtcaat ttatcttctc agataatcac
    14461 caaagttttt tcaatactca catgtccact tactatgttg aagctccatc tacttttaac
    14521 gaacttttac ttagtgacta cttagaaaac caatttgata cagcacgtca aaaacgcttt
    14581 gctcttgcac atcgcttaac tgatacttac ttccacaatt tcattactca cttacttgaa
    14641 gctgctttcc aacgtaaagt ttacactcta attgaggagg gagaaacttt tggagcagaa
    14701 caattaaatg ctattatgaa ggaagtatta acacaattct ggggagatgc tattgaaata
    14761 gatgacgatg ctgctttgac atggatgaga caagctcatt actacatggg gctctatagc
    14821 tatacttact ctgctggttt agtaatctcg acagccggct atctcaatct caaaaataat
    14881 ccaaacggag caaaagagtg gttagcattc ttgaaatctg gtgggagctg taccccttta
    14941 gaaacagccc tattaatcgg tgctgatatt tctactgata agcctctacg cgatactatt
    15001 aatttcttat ctaacactgt agatcaaatt attaattata gtaatcttat taaacaaggc
    15061 taaactacag tctttcaaaa acataaaaac aagttggaga tatctccaac ttgtttttat
    15121 cttatttttt gtaaacttcc tgataaaatt caattttgtc gttcttttta actttaattt
    15181 ggtttgctgc cttaggagct aatttattat tgaccttgaa catccaataa agccctttgg
    15241 tttcatcttg gctgatgcca tcaattgaag taatgaaacc atctttttct ttaactttat
    15301 agttagcttt aagcacatcc aaaacagtgt cacctttatg gtaaacaacc ttttcactta
    15361 acacgttcgt atcttcttta acaaccaatt caaccttacc aacttctaac gttgagctag
    15421 ttgttgtctt attcgatgta gatggttgcc cgcaagctac taaagtcgta gcgaatactg
    15481 ttagcaagaa taatttaatg tattttttca ttcctaaatc tccttaaaat cggtaacaaa
    15541 agaggataaa ataaacaagt tgataaagcg tgtgccatat taaaactaag tcctgctaat
    15601 acataagtcc accaaggcat atgatataac caagcaaaac aggtatcaat aatgacgcca
    15661 taaacaaccc ctaaccctcc tgcaaagaaa gtttgaagta ctacctccgt tattttacca
    15721 aaacatacag cttttgtcag cggatatatc agaaatttcc acaagcataa aattaaagtg
    15781 aaggaaatga tttgcaaaaa gacccatggc ccaaagccca ttaaaaaaga agaaactagc
    15841 attgttacag ttacagtagc tacaccttct tttaaatcga ataagacaac tgtaatcaaa
    15901 aaaatggcag taattggctg aatgttaggc agaggcgcaa aagcatatct caacacaaca
    15961 cacaaagcag ataatatagc tactctagtc aatttagtta aagttgaatt ctttatcatg
    16021 tctttaatta tacctcaaaa ctatgactac cgcttaaaag tcttttttat tacaatttca
    16081 tttctaatgt tacataaacg acctcttttt ttaaaagaac gtaaataagc ctgtaaactt
    16141 tgatttttta aaccttttaa gttacaatat caataatgag aagaagaatt aaacctattg
    16201 ttgttgcggt ttttttcagt ttatttggac ttttattaat tattggtcat ttacattcga
    16261 caaatacttt gaaaaaagaa ttagttgaag ctaaaaagac aattccatcc gtaaaagctt
    16321 caaaagtacc gcaaaaatca acatcatcga aagataaaga gtttgttctt aaaccgatta
    16381 tcgatgtctc tggttggcaa cttcctaagg agattgatta cgatacgctt tcaaaaaata
    16441 tttcaggtgt tgttattcgt atctttggtg gatcaaagat atctaagact aataacgctg
    16501 cttatacaac tggaatcgat aaatcgttta agacccatat caaagaattt caaaagcgaa
    16561 atatcccagt agctgtctac agttatgcac ttggttcaag tgttaaagaa atgaaagaag
    16621 aggctcagat attttataag aatgcagctc cttacaaacc aactttttat tggattgacg
    16681 tagaagagga gacaatgtct aacatgaata aaggtgtcca agcattccga aaagagttaa
    16741 aaagacttgg tgctaaaaat gttggtatct acatcggtac ttactttatg actgagcaag
    16801 gtatctctgt aaaaggattt gacgctgttt ggattccaac ttatggtagc gattctggat
    16861 actatgaagc agctccacaa actgaactta aatacgattt acaccaatac acctctcaag
    16921 gttatctacc aggattcaat caaccgcttg atttaaatca aattgcagtt aataaagaca
    16981 agaagaaaac ttatgagaaa ctttttggaa aagtaaaaga ttaaaagcta ctgttaacag
    17041 tagctttttt gataaactat ttattattta attcttcaat agaaaggatt ttttgagttg
    17101 gttttttcct aagcgaagta acgattgctt ctgccactat cacaacctta gttggaaaga
    17161 ctttttgtac aacaatacct aagaaaggca aatgactaaa taattttata cacttggctt
    17221 ggaaaatcga gagaggtcgc tcttcaccat acatcaaacc tggtcttaca aaaagataat
    17281 ccagaccgct tgctttgatt atctgctctg ccttcctttt acttttaatg taagctgaat
    17341 agccgctatt ggctgaaatg taaactaact ttggtatttg atttttgtga cagagtgcta
    17401 ctgctttttg ggttgcttta acgttaagct catctagttg attgggctta atcgctccaa
    17461 tacagtcaat taatatatca aaatttctat gttctaaatg aatcttatca gcttctgtaa
    17521 tatctccctt aatgtaggtt aatctaggat ccttaaatat atcaccttta ccttcatgcc
    17581 ttgataagta agccacctta tgcccttttg taagcgctgc ttttattatc tgcttcccta
    17641 gaaaaccact accacctgca atcagtattt ccatagttcc ctctccttca ttttgtaaat
    17701 ttagtataga aatttttata tgccttgtca aagtaatcgt ttaatacctg accagaatat
    17761 gatttcaagt cattacatta ggacacttca tatttagcta tttataaaac ttctaattta
    17821 acattccaaa tatactgttg cgatatttat tatcattatt ccctgaaatg gtattcaagc
    17881 ttatttctaa gactcagtag aaatttacta taatcatctt taaatgttat aataaatatg
    17941 aaacaaactt attagttaag ggtcattatg agtaaaaaga ataaaatcaa aaaaacttta
    18001 gttgatcaaa ttttagataa agcaaaaatc gaacatgata gtctacaatt ggatgcttta
    18061 cagggagatt tacccaacgg tattcaaaaa caagatatct ttaagacatt agcgctaatc
    18121 ggtgataaaa ctggtcccat aataggtatc ttacctctca ccgagcattt atccgaaaaa
    18181 aagttagcta aaatttcagg aaacaaaaag gtacaaatga tacctcaaaa agatctccag
    18241 aaaataacag gttatattca cggagcaaat aatccaattg gaattcgcca aaaacataat
    18301 taccctattt ttatcgatac tatcgcttta gaaaaacaag aactcattgt ctctgcagga
    18361 gaaattggac gttctattcg tattaatagt gaggttcttg cagatttcgt aaatgctaaa
    18421 tttgccgata taaaggaata ataattagga ttggagaaaa atatgaaatt ttatcttgtt
    18481 agacacggaa aaacacaatg gaatttagaa gggcgtttcc aaggtgctaa cggagactca
    18541 cctctccttg aagaagctat tgaagaatta gaagaacttg gacaatacct atcatctatt
    18601 cattttgatg ctgtttattc tagcgactta ggtcgtgcta gagatactgt taatatcctc
    18661 aatgatgcta attcatgccc aaaagaaatc cactatactt ctcagcttcg tgaatgggca
    18721 ttgggaactt tagagggttg taaaattgca actatgcaag ctatctatcc tcgtcaaatg
    18781 accgcatttt atcaaaatcc cttacaattt aaacatgaca tgttcggtgc agaatctctt
    18841 tatcagacaa cgcatcgtgt agagagtttc ttacgttctt tagcttcaaa aaattacgac
    18901 aaggtcctta ttgtaggaca tggagcaaat ctaactgcgt ctattcgttc cttactgggt
    18961 tatcaatatg gtagtttaca ctacaaagat aaacttgata atgccagcct cacaatcata
    19021 gaaactcatg attttaaaga cttcaactgt ctgacttgga acgataaatc ttatttaagg
    19081 caagaggtca aaatgacaca ctaaaataat ccactagatt catctagtgg attatttaat
    19141 tatctagcat acttcatgcg taatactttt tcctgttttg cagttaggcg cagtaaaata
    19201 acaactttgt caacgctcat attactctca agcagacgct ctgatgccat cattaaacca
    19261 ttattaagtc ccatttcaag gactggatta cttttatcgt tgatatcaaa aataaattta
    19321 ttatcaggta agtcaaacaa cactttaaca gcaccgaata attgctcaaa gttatcaata
    19381 gcaacatcat aaactggttt aattcccggt tttaaagaac gtcctctatg gttgaaccac
    19441 atagaatgcc aaccaccatt aaatgcaccc ataatatcat tatcatatga atcacccaca
    19501 taaagtgtag ttgaagggtt catatcaaat tgctctgctg ctaaattaaa aatctccttt
    19561 tcaggctttt gaaaaccagt tgcttgacta actataacac gttttggatc aacatagtca
    19621 taaagtccca attttttaac ttttttcaat tgatgttccg tagggccatt tgtaataatt
    19681 cccattggta cattttttga tttcaaaaag tcaagtgtca tacgcatctc atcaagcatt
    19741 gtaatatttt caagttcatg ttcgtatatt tcttgaaaat aaataccagt agcttcatca
    19801 atttcacgat aaccaaactc taaaagcgtt tctttgcatc gccagaaacg aaaatattct
    19861 gttgtccatt caccagccat tactcgtgga aagccaatat ctgaataatg acgaaagcga
    19921 atataagctt ggttaatagc gctcatatca aaatcaggaa agcatttctc cacagcaata
    19981 cggtaaggtg cttgttgatc gtaaattgta tcatcaacat caaaaacaat tgaagtaatc
    20041 atgattatcc ttttctttct gaattttcaa ttgttaatta tacctttttc tacccttatt
    20101 ttcaaatggt gaaaagtcta ttaaaaatga gaataacacc ataaactcat aaaataaatt
    20161 atgaattatc agtgtttttg tggtacaata ggatgaatac taacttggag gaaattatgt
    20221 ctaaccaaca tattgaagaa ttaaatgacc aacagattgt tcgtcgtgaa aaaatggctg
    20281 ctttgactga acaaggcatt gatccttttg gtaaacgatt tgaacgtaca gcaacatctg
    20341 gtcaattaaa tgaaaaatac gctgacaaat caaaagaaga cttgcacgac attgaagaaa
    20401 ctgctacaat cgctggtcgt ttaatgacta agcgcggtaa ggggaaagtt ggctttgcgc
    20461 atatccaaga tcgcgaaggt caaattcaaa tttatgtccg taaggattct gttggcgaag
    20521 aaaactacga aatctttaaa aaagctgatt taggtgactt ccttggtgtt gaagggcaag
    20581 taatgcgtac agatatggga gaactttcta tcaaggctac tcatattact cacctttcaa
    20641 aagcactccg tccacttcct gaaaaattcc acggcctaac tgatattgaa acaatctatc
    20701 gtaaacgtca tcttgattta atctcaaatc gtgacagctt tgaccgtttt gtaacacgct
    20761 caaaaatcat ttcagaaatt cgtcgcttta tggatagcaa tggtttctta gaggttgaaa
    20821 cacctgttct tcataatgaa gctggtggtg cttctgctcg cccatttatc acacatcaca
    20881 atgcacaaga cattgacatg gttcttcgta tcgcaactga attgcatcta aaacgtctta
    20941 ttgtaggtgg tatggagcgt gtttatgaaa tcggacgtat tttccgtaat gaagggatgg
    21001 atgcaaccca caatccagag ttcacatcta ttgaagccta ccaagcctat gctgactacc
    21061 aggatattat ggacttgaca gaaggtatta tccagcatgt tacaaaaact gtaaaaggtg
    21121 atggaccaat caattatcaa ggcacagaaa ttaaaattaa tgaaccattc aaacgtgttc
    21181 atatggttga tgcggttaaa gaaatcactg atattgactt ctggaaagaa atgactcttg
    21241 aagaagcaca agctttggca caagaaaaaa atgttcctct tgaaaaacat ttcacaacag
    21301 ttggtcacat cattaacgcc ttcttcgaag aattcgttga agacacttta attcaaccaa
    21361 cttttgtctt tggtcatcca gtcgaagtgt ctccacttgc taagaaaaat gatacagacc
    21421 ctcgttttac agaccgtttc gaactcttta tcatgactaa agaatatgct aatgctttta
    21481 cagaattaaa tgacccaatt gatcagctat ctcgctttga agcacaagca tctgctaaag
    21541 aacttggcga tgatgaagca actggagttg attacgatta tgttgaagca cttgaatatg
    21601 gtatgccacc tactggtgga ctaggtattg gtatcgaccg tctctgtatg ctgttaactg
    21661 acacaacaac tatccgagac gtcctacttt tccctacaat gaaataagaa tatattaaaa
    21721 cccaccctta tttaaggtgg gttttaatat tacctctact gaactttgtt aggtttctga
    21781 tttatcgctc acgcaatttt ttatcaaggt ttaccatttc aatagccgaa agagcacaat
    21841 cataaccttt atttcctgct tttgttcctg ccctctcaat agcttgttcg atattatccg
    21901 ttgttagaac accaaagata tgagggatat ctgactggct atttaaatga ccaatacctt
    21961 ttgtcacctc attacaaaca taatcataat gatcagtgct gcctttaatg actaccccaa
    22021 gacagataat agcatcataa tctttatata atgccatttt tctagccata taggggattt
    22081 caaaagcacc gggtacccat acaatatcta tatcttcttc tgaaacacca tgtctcaaca
    22141 gtccatctac tgctcctgat aataatttag atgtaatcaa ttcattgaaa cgtgagacta
    22201 cgattcctat tttcatttcg tttgccacta attgtccttc aataattgtc attttttctt
    22261 cctcctttaa tctaaaagat gtcccatttt ttctttttta gtttttaaat aattaaggtc
    22321 ataagctgtt gctgccacct gtaatggcac tctgtttttg acgcaaattc cttctgcttc
    22381 aagttgttgg attttatctg gattattggt tagtaagttt atagaattta tacctaaatc
    22441 ttttaaaagt tgagcactaa ctccatagtc tctctcatct ccttcaaaac ctaaagccaa
    22501 attagcttct aaagtatcta agccttcttc ttgcaaatga taagctttta acttattaat
    22561 caaaccaatt cctcgacctt cctgtctcat ataaagaagg acgccacgac cttcagcctc
    22621 tatttgttgg agtgcattag ccagttgttc cccacaatca catctcaagg aatgaaagac
    22681 atcacctgtc aaacattctg agtgtaaacg acataaaaca tcttcaccac tactaatatc
    22741 ccctttaaca agggcaacat gttccttatt tgccacaact tcactatatc cataagcctt
    22801 aaaatggccg aattgcgttg gtagctgaat ctcaatttct ctaaccaccc ctccctcttc
    22861 ttgacgtcta tagtcttgca attgttttat ggtagcgata gctaaatcgt gcttttgagc
    22921 aaatgctaat aactcatcct ttctcatcat actaccatct tcagccatta tttcacaaca
    22981 aagtccacat tcctttaatc cagctaaacg acatagatca acggttgctt cagtatgtcc
    23041 atttctagct aaaacacctc cttccttagc taacaaggga aataagtgcc caggacgtct
    23101 aaagtcttta gctttgctac tgtcgttagc aaccatctta gctgtcaaag ctctatcttc
    23161 cgcagaaatc ccagtactag ttgatagatg atcaatggat atggtaaaag ctgtttcatg
    23221 attatcagta ttatggctag ccatttgagc taattctaac ttttcagcat agtaattaga
    23281 cagaggtaag caaattaatc ctttcccaaa tttagccata aaattaatcg actcttttgt
    23341 caccatttca gccgcacaaa taaggtcccc ttcattctct ctattttcgt catccatcaa
    23401 aactaccatt tcccctgatt tgatgtcttg taaaagtttt tttattggac taaaggccat
    23461 tcgctttctc ctctctctta gtagccattt tctgataaaa aagaccaatc cattactgat
    23521 gaagtgtgat tagttctatt agtcggtgct aaaaatttct cagcatattt gcctaaaaca
    23581 tccacttcga tatttaataa atccccaacc ttttttaagg ataaattagt ttgagccatc
    23641 gtgtggggaa cgaccgaaac ttcaaaagaa tgctctgtaa atgataccac cgttaggcta
    23701 atcccatcta gagccaccga acctttctct ataatataag acataatact gggcggagca
    23761 tcaattgaaa atctgacagc aatatcatcc ttttggattt tttctatttt tccaacccca
    23821 tccacatgtc caagaacaaa atgcccccct aaccttgagt cacttctgag tgctaattct
    23881 aagttaactt tagaatgaag tcgtaaagca gctaagcttg ttcgcatcat actttctggc
    23941 atgacgtctg ctataaaagc tttttcactt ttgtcaagga ctgtcaaaca aacaccatta
    24001 acagctacgc tatccccttt tcttaataaa gggactagtt tgggaacatc aatacttaat
    24061 tgttgggatt ttatgccatt ccgaatacga ctgacttgac ccatttcctc aataattcct
    24121 gtaaacataa tgttctacct ttccttcaat aacaacgtca gaatccatac gactaagttc
    24181 aatatctttt actctgaaag cttggttaag ccaatccaag ccttctccag aaatagctgt
    24241 ttttgctttc agacctccta ttattttggg tgcaataaaa acaattagtc tgttaacaat
    24301 accagcttct aaagctgaaa aatgtaaact acttcctcct tcaataagaa ggctatcaat
    24361 cccttcttgt cctaaaatag tcattaatac tttcaaatcc agctttccat cttttcttgg
    24421 gaccttaatc aaacggcaac ccatttcttt taatgtttgt tgctgagcaa gattatctga
    24481 acatgttgca atccatgttg gtattgtttt agcgctctta actaggtaag agtctaaagg
    24541 tatcttcaac tgactatcac atacgatcct cacaagcgct tcacctttag gaatacggca
    24601 agttaaacgt ggattatcag ctaatacagt attgataccg accataatag cactacattt
    24661 ttgcctcaat ttctgaacag attgtcttga gtgctcattg gatatccact tagaatcgcc
    24721 cgtctttgta gctatcttcc catctaaagt catagcatat ttcaaataaa caaaaggctg
    24781 cttataggtc atgtgaaaaa taaatctttc attcagagca tcgcattctt ctcttaggat
    24841 accaacttca acatttaaac cttcttttct taacaaggca atcccttttc ctgaaactaa
    24901 aggatttgga tctaaacttc caactactac ttttttgata ccagatttaa taagagcttc
    24961 agtacaaggt ggttgcttac ccacatgaca gcatggttct aaagtcacgt acagagtact
    25021 tccagagata tcctcatcag catttttaat agcctgtctt tccgcatgta aatctccaaa
    25081 tcgtttatga taacctttgg aaattatacg atcatcttta acaataacag cacctactaa
    25141 tgggttggga gcaacgaacc ccataccttt ctcagcctct ttcaaggcta atgccatgta
    25201 gtcttctttt ttcatcattt cctttttcct tctaaaataa aaacccttag cttctaaaat
    25261 agaaaactaa ggggagggat gagaagtcgt tgattatact aattagcatt atattttagg
    25321 tagcacaaaa aaactaaaga ctccctatag aaaactatag ggagtctctc attaatccag
    25381 cacaaataaa ctgcaagtag cagacaatca ctgaattctt ctctcatcca gactttactg
    25441 tcggtactgg aatcacacca gttcatgctc gaaagctcgc ggactatacc gccggttagg
    25501 attttcacct gaccccgaag aaattctatt tgacagttat tttactaaac tcatttttat
    25561 ttgtcaataa aaataatgtg ttttttatta taaaataata attaagtagc tcatctattc
    25621 ttatctttaa aagacagtat atctatacca taaagcaat
//
LOCUS       DATRNX010000017        25465 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRNX010000017 DATRNX010000000
VERSION     DATRNX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25465
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(170..1556)
                     /locus_tag="VBC81_000571"
                     /pseudo
     CDS             complement(170..1556)
                     /locus_tag="VBC81_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            1697..2500
                     /gene="proB"
                     /locus_tag="VBC81_000572"
     CDS             1697..2500
                     /gene="proB"
                     /locus_tag="VBC81_000572"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6911633.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            2510..3763
                     /locus_tag="VBC81_000573"
     CDS             2510..3763
                     /locus_tag="VBC81_000573"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6911634.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGTKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            3846..4793
                     /gene="rsmH"
                     /locus_tag="VBC81_000574"
     CDS             3846..4793
                     /gene="rsmH"
                     /locus_tag="VBC81_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6911635.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            4808..5134
                     /gene="ftsL"
                     /locus_tag="VBC81_000575"
     CDS             4808..5134
                     /gene="ftsL"
                     /locus_tag="VBC81_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6911636.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            5138..7396
                     /locus_tag="VBC81_000576"
     CDS             5138..7396
                     /locus_tag="VBC81_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6911637.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            7398..8408
                     /locus_tag="VBC81_000577"
     CDS             7398..8408
                     /locus_tag="VBC81_000577"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6911638.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            8506..9849
                     /locus_tag="VBC81_000578"
     CDS             8506..9849
                     /locus_tag="VBC81_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6911639.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            9987..10799
                     /locus_tag="VBC81_000579"
     CDS             9987..10799
                     /locus_tag="VBC81_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6911640.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            10894..11697
                     /locus_tag="VBC81_000580"
     CDS             10894..11697
                     /locus_tag="VBC81_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6911641.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            11697..12440
                     /locus_tag="VBC81_000581"
     CDS             11697..12440
                     /locus_tag="VBC81_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6911642.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            12562..12786
                     /locus_tag="VBC81_000582"
     CDS             12562..12786
                     /locus_tag="VBC81_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6911643.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            12855..13769
                     /gene="trxB"
                     /locus_tag="VBC81_000583"
     CDS             12855..13769
                     /gene="trxB"
                     /locus_tag="VBC81_000583"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6911644.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            13927..15387
                     /locus_tag="VBC81_000584"
     CDS             13927..15387
                     /locus_tag="VBC81_000584"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6911645.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            15384..16205
                     /gene="nadE"
                     /locus_tag="VBC81_000585"
     CDS             15384..16205
                     /gene="nadE"
                     /locus_tag="VBC81_000585"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6911646.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            16318..17652
                     /gene="pepC"
                     /locus_tag="VBC81_000586"
     CDS             16318..17652
                     /gene="pepC"
                     /locus_tag="VBC81_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6911647.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(17699..19939)
                     /locus_tag="VBC81_000587"
     CDS             complement(17699..19939)
                     /locus_tag="VBC81_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6911648.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(19926..20525)
                     /gene="recU"
                     /locus_tag="VBC81_000588"
     CDS             complement(19926..20525)
                     /gene="recU"
                     /locus_tag="VBC81_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6911649.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            20600..21118
                     /locus_tag="VBC81_000589"
     CDS             20600..21118
                     /locus_tag="VBC81_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6911650.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            21239..21571
                     /gene="gpsB"
                     /locus_tag="VBC81_000590"
     CDS             21239..21571
                     /gene="gpsB"
                     /locus_tag="VBC81_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6911651.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            21590..21958
                     /gene="rnpB"
                     /locus_tag="VBC81_000591"
     ncRNA           21590..21958
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC81_000591"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            22041..23195
                     /locus_tag="VBC81_000592"
     CDS             22041..23195
                     /locus_tag="VBC81_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6911652.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            23208..24695
                     /locus_tag="VBC81_000593"
     CDS             23208..24695
                     /locus_tag="VBC81_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911653.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(24920..25402)
                     /locus_tag="VBC81_000594"
     CDS             complement(24920..25402)
                     /locus_tag="VBC81_000594"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6911654.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
BASE COUNT         8404 a         4045 c         5123 g         7893 t
ORIGIN      
        1 cttaataact gagatagtta tagcggataa aataaaggct tgttgtggac tttacggttg
       61 gaaggcagcc actgtaatcc tttgtttaat aacgctagtt ttagctctaa aatatttgat
      121 ttttattgct atctattata tcttatcaga caaagagact agggataacc tagtctcttt
      181 gtctgatgct aacttgtttt tcgtaatatt gtgctagaat gaatgtcaat atagcagttg
      241 tgcttacagt aatgataact gcaataagta tggtaataaa aagatgtgtt gaagtatgac
      301 tgatataaag aaatagacct ggtagaccag aagagcctac agcgaagcgg tgagcgttca
      361 ttataccggc atagagtcct ccaagaccac caccaataag tgaggctatt agaggataac
      421 gtttcggaag gttgacacca tataagatag gttcggtgat tcccatataa gctgttatgg
      481 cagcaggtcc agcaatttct ttaagctttt tttcttttgt tataagagtc accactgtac
      541 ctgcagttgc ttgtgcgata tttgagcaga gcatgcctgg tccaaaaata gaatcaaccc
      601 ctaatttcgc tagttgtgtg attcctaatg cagcaattcc actatgaaca ccaaacatga
      661 ttaggatagg agcaaatgct cctactagaa aagcaggtgc ccaactggcg tatttggata
      721 aaaaaagtaa aaataacagc taaatattgg ccagcgatag tacccattgg tcccaatata
      781 gagaacgaca aaattcccat gctgagaaat attaacatag gaacaagcac taaacgtagt
      841 gatttaggaa taatttgatt taaatatttt tctatataag cctgaacaca aataattaga
      901 ataatgggaa tgacacttga tgcatagtta gtaagtgtga atgggatcgt atggaataag
      961 gatactggtt taccagaagc tactaaatta gcccaattag ggtgaaggag catgacaaca
     1021 gttcccaaag ctaaaatagg attagccttc aatttttgag cagctgtgat ggcgattaag
     1081 ataggtaaaa aatagaaaac accatctgca aaaagattta gtaatatata tgtttgagat
     1141 aacggtgtca agattttgaa tactagtaat aaagctagca atgccttaat cataccagcg
     1201 cctgatagag ctggtaaaat cggttggaaa gtcccagaaa caaaatcaat aattcgacta
     1261 actgctttgg cttttttggt ttgttctgtt ttagtcgttg agttagtttc aatgagggag
     1321 ttaatttctt caaacacttt tgcgacatga gtcccgatca ctatctgatg ttgccctccg
     1381 ctgataacga cagtttttac ctcttttaaa gtttttaatt gctcaaggtt tactttcgtc
     1441 ggatcattga gactaaagcg aaggcgtgtt tgacaatggt aaacattgtt aatattattt
     1501 tgcccaccaa ggtgtttgat gatatttttt gacaaggtga tgtattctct agacataata
     1561 tctccttaat acttataaaa cgttttccga ttctagatta tagcatagta ataaaagaaa
     1621 acacttggaa atgcttgtga attttcaatg atgttttcag atagcattcc taaagggtct
     1681 atgatataat ttttttatga aaagacattt tgagacaact agaagaattg ttattaaagt
     1741 aggaaccagt tccctcgtac agacaagtgg gaaaattaat ttaagtaaaa tagaccattt
     1801 agcatttgtt atttctagtc ttatgaataa aggtatggag gtgattttag tatcatctgg
     1861 agctatgggg tttggtttag acattttgaa aatggataag cgtcctcaag aaatatcaca
     1921 acaacaagca gtttctagcg taggacaagt agccatgatg agtctttatt ctcagatatt
     1981 ttcgcactat caaactcatg tttcccaaat tttattaaca cgtgacgttg tggtttttcc
     2041 agagagtttg caaaatgtaa caaactcttt tgagagtctg ctctctatgg gaattttgcc
     2101 aatcgtcaat gagaatgatg ctgtcagcgt ggatgagatg gatcataaaa cgaagtttgg
     2161 ggacaatgac cgtctctctg ctgtcgttgc taaaattact aaagcagatt tattaattat
     2221 gctgtcagat attgatggtc tttttgataa aaatcctaat atttatgatg atgctgtttt
     2281 acgtagtcat gtttctgaga tcaccgatga catcattaag tcagctggag gagcaggaag
     2341 taagtttggt actggtggca tgcttagtaa aattaaaagt gcacaaatgg tgtttgataa
     2401 caatggacaa atgatattga tgaatggcgc caatcctcgt gatattttaa aggtgttaga
     2461 tgggcataat attggaactt attttgctca aggaaagtga gattgagtta tgacgtatat
     2521 tgaaatatta gggcaaaatg ctaaaaaagc tagtcaatca gtagcacgat taagtacagc
     2581 tagtaaaaat gagatactca gggacttagc gagaaacata gtggctgata ctgaaaccat
     2641 tttgactgag aatgctcgcg atgttgctaa ggcaaaagat aatggtatct cagaaattat
     2701 ggtggaccgt ctacgactaa acaaagaccg catacaggcg atagcaaacg gtatttatca
     2761 ggtagcagat ttggcagatc cgattggtca agttgttagt ggttatacaa atttagatgg
     2821 tttaaaaatc cttaaaaaaa gagtgccttt aggggttatt gcaatgatat ttgaaagtcg
     2881 tcccaatgta tcggtggatg cctttagttt agcttttaaa acagggaacg ctattatttt
     2941 acgtggtggt aaagatgcta ttttttcaaa tactgccttg gttaattgta tgcgccaaac
     3001 attgcaagat acaggccaca acccagatat agtacaatta gttgaagata ctagtcatgt
     3061 tgtagcagag gagttaatgc aggcaacaga ctacgtggac gtcttaattc caagaggtgg
     3121 tacaaaatta attcaaacgg tgaaggaaaa gtcaaagatt cctgttatcg aaacaggtgt
     3181 tggaaatgtt catatctata ttgatgaatt tgcagattta gatatggcag ctaagattgt
     3241 tattaatgct aaaacccaac gccctagtgt ctgtaatgct gctgaagggt tagttgttca
     3301 tcaagctatt gctaagggat ttttatctca attggagaaa atgctcaaag aatcgaacca
     3361 gtcagttgaa tttcgtgccg atgaggaagc tttacaactt ttagaaaatg ctgttgctgc
     3421 aagtgagact gattatgcta ctgaattttt agactatatt atgtcagtaa aagtagtaga
     3481 ttcttttgaa caagcaatta gctggattaa taaatacagt agtcatcact cagaagctat
     3541 cattactaat aatatcagta gggcagaaat ttttcaggat atggtagatg cagcggcagt
     3601 ttatgttaat gcttcaacac gttttacaga tggtttcgtt tttggactcg gagcagagat
     3661 tggtatttcg actcaaaaat tacacgcgcg tggaccgatg ggattagaag ctttaacaag
     3721 tacaaaatac tatattaatg gaactggtca agtaagggaa tagttgacct ataataggag
     3781 agattgcatt tatcggtctc tcttttactt ttctaaagtt taggatttat ggtacaatta
     3841 aagttatgac aaacgatttt catcatatta ctgtgctttt gcacgagact gtggatatgc
     3901 ttgatattaa gccggatggt acctatgtag atgctacttt aggtggggca ggacacagcg
     3961 aatatcttct ttcacaatta ggcccagatg ggcaccttta cgcatttgac caagaccaga
     4021 aggctattga cattgctcat atacgtttaa agaaatatgt ggataccggt caagtaacct
     4081 ttatcaaaga taatttcaga aatctatctt ctaacttaaa agcattagga gtgtcggaaa
     4141 ttaatggtat ttgttatgat ttaggtgtat caagcccaca gctggacgag cgtgaacgcg
     4201 gtttctcata taaacaagat gccccccttg atatgcgaat gaatagagag cagagcctta
     4261 cagcttatga cgttgtgaac acttatagtt atcatgattt agtaaggatt ttctttaagt
     4321 atggcgaaga taaattttca aaacaaattg ctcgaaaaat tgagcaagta cgagctgaaa
     4381 aaccaatatc tacaacaaca gaattggctg agattatcaa atcgtctaaa tctgccaaag
     4441 aactgaagaa aaaaggtcac ccagctaaac aaatttttca agctattcgt attgaagtca
     4501 atgacgaact aggagctgca gatgaatcta tacaacaagc aatggactta ttagctgttg
     4561 atggtcgtat atctgtcatc acttttcatt ctttagaaga tcgtcttact aagcaacttt
     4621 ttaaagaagc atcaacagta gaagtaccta aaggtcttcc ttttatacca gatgatttac
     4681 aacctaagat ggagctcgtt aatcgtaaac ccatcttacc aagtcaagaa gaactagaag
     4741 cgaacaatcg tgcccattca gcgaaattga gagtagcgcg tcgtattcga taataagaaa
     4801 gaaacttatg acaaatgaaa aacgaactga agcagtaaca caaaccttac aacgtcacat
     4861 caagactttc tctcgcattg agaaagcttt ttatggcgct attgtaatta ctgctattat
     4921 catggcagtc ggtattattt acttgcaaag taatagttta caagttaaac aagaagtaaa
     4981 tcaattaaat agtaaaatca acgataaaca gacagaattt gacaatgcta aacaagaagt
     5041 aaatgagctt tctaatcgtg atcgaattac taaaattgct aaagatgctg gtttaacaat
     5101 tcaaaacgac aatatttatc ggaaggtgga ctagtctgtg acttttttta aaaagctaaa
     5161 aaaaatcttc cttgattatg tgatccatat aagagatagg cgttcgccgc agaaaaatag
     5221 ggaaagagta ggtcaaaatc tgatgatttt aaccattttc ctatttttca tttttatcat
     5281 caactttgtc atcatcgttg gtacagatag taaatttggg gttaatttat ccaaggaagc
     5341 caagaaagtc tatcaacaaa gtatgacagt acaggcgaag cgtgggacaa tttacgaccg
     5401 taatggaaat cctatcgctg aggatgctac gacctatagt ctctacgcta ttatctcaaa
     5461 gaactatacg acagctacag gtcaaaaact atatgtacaa ccttctcagt atgaaaaagt
     5521 agctagtatc ttagagaata aattgggcat gaagaaaaat cttgtcctaa aacaactcaa
     5581 tcaaaagaaa ttgtttcaag tttcctttgg aagttcaggt tcaggattgt cttataccaa
     5641 gatggctgac atcaagaaaa cgatggaaaa gtcagatatc aaaggaattg gtttttctac
     5701 cagtccgggg cgtatttatc caaatgggat atttgcttca caatttatag gatttactct
     5761 tcctcaggat gatggagatg gaaaaaaatt agttggtaat acaggccttg aagcggcact
     5821 taacaaggta ttatcaggta ctgatggcaa agtaacatac gaaaaagacc gtagtgggaa
     5881 tgttctttta ggtactgcaa caacagaacg tagggcggtt aatggtaagg atatctacac
     5941 aactttatca gagcctattc agactgtttt agagactcag atggatgttt tcgcagaaaa
     6001 aaccaaagga aaatttgcga gtgctacagt tgtcaacgct aaaacgggag aaattctagc
     6061 aacgagtcag cgtcctactt ataatccttc tacccttaaa ggttacgata agaaaaattt
     6121 gggtacctat aacacactac tatatgataa tttttttgaa cctggttcaa caatgaaggt
     6181 aatgactcta gcctctgcaa ttgattctaa gcattttaac tctactgaag tgtataacag
     6241 tgcacaatat aaaattgcag atgcggttat tcgagattgg gatgtcaatg aagggttatc
     6301 atctggtagt tatatgactt ttccacaagg gtttgcacat tccagtaacg ttggtatggt
     6361 aaccttggaa caaaaaatgg gacgagataa atggttaaac tatctttcta agtttaaatt
     6421 tggttaccca acacgttttg ggatgcttca tgaaagtgga ggtttgttcc catctgacaa
     6481 tgaagtaact atcgctatga gctcatttgg tcaaggtatt ggtgtaacgc aagttcaaat
     6541 gttgcgcgca tttacgtcta tctcgaatga tggtgttatg ttacaaccac agtttattag
     6601 cagtatttat gatccaaata cagggacttc tcgtacagca cgaaaagaag tcgttggaaa
     6661 accagtttcc aaagaagcag ctagtaaaac acgtgattat atggttactg ttgggacaga
     6721 tccttattat ggtacattat acgcagcagg tgccccagtt attcaggtgg ggaatcaatc
     6781 agttgcagta aaatctggta ctgcccaaat cgcccaggaa ggtggtggtg gttatctgca
     6841 aggaaaaaat gatactatta actcagttgt ggctatggtg ccatcggaaa atcctgactt
     6901 catcatgtat gttaccatcc aacaacccga aaaatttagt atcacattct ggaaagatgt
     6961 tgttaatcct gttttagaac aagcaacagc aatgaaagaa acgatattaa aaccggtatt
     7021 gaatgactca gaacatcaga caaaatataa actatcaaaa atagttggtg aaaatccagg
     7081 tcatgtagcg gaagaattac gtcgtaatct ggttcagcca attattttag gaaatggtag
     7141 taaagtttct aaagtatcta aaagaccagg agctaatctt gctgagaatg aacagctact
     7201 agtattaaca aataaactga ctgaattacc tgatatgtat ggttggtcta aagcaaacgt
     7261 tgaacagttt gctaaatgga caggtataaa agtgacctat aaaggttcaa cctcaggtaa
     7321 agtaagaaaa caaagtattg atgttggaaa atccatcaat aaaattaaaa aaatcaaaat
     7381 tacaatagga gattaacatg tttttaagta taatggcagg tgtcatagca tttgtcctga
     7441 cagttattgc cattccacgc ttcattaagt tttaccaatt gaagaaaatt ggcgggcaac
     7501 aaatgcatga agatgtcaaa caacatctag ccaaagcagg tacgccgaca atgggaggaa
     7561 cggtattttt ggttgttgct cttcttgtct cacttatttt tagtattatt ttatctaaag
     7621 aaaatagtgg taatttaggt gcaacttttg gtattttatc tgttgttctc atctatggaa
     7681 ttattggttt tttagatgat tttttgaaaa tttttaaaca aatcaatgag ggcttgactc
     7741 caaagcaaaa aatgtcgctc caactaattg cgggtctaat tttttatttt gtccatgtgt
     7801 taccaagtgg cacatctgcg ataaatattt ttggttttaa ccttgaagta ggatacttat
     7861 acgctttttt tgttcttttc tgggttgttg gtttttcaaa tgctgtgaac ctaacagatg
     7921 gaattgatgg tctagcttct atttcggttg ttatcagttt gattacttat ggaatcattg
     7981 cttataatca aacgcaattt gatattttat tgattatcgt tatcatgatt ggtgctttac
     8041 ttggcttttt tgtctttaat cataaaccag ctaaagtctt tatgggagat gttggttcac
     8101 ttgcattggg tgctatgtta gcagccattt caattgcctt acgtcaggaa tggaccttgc
     8161 tttttatcgg ctttgtctat gtttttgaga cgtcttcagt gatgttgcaa gtagcatact
     8221 ttaagtatac taagaagaaa actggtgttg ggaaacgtat cttccgcatg acaccatttc
     8281 atcaccattt agaattaggt ggtgtgtctg gcaaaggtaa taaatggagc gaatggaaag
     8341 tagatgcttt tttatgggct attggtattt tcatgagcgc tattacactt gctattttat
     8401 atctttaaac tttattaggg gatgtggtat ggactgccag attccctttt gctttcttgt
     8461 agcctttaat ggtataatga acataataat agtaaggaaa tagacatgtc atttaaagat
     8521 tttaatttta aaccatacat tcagagggct ctcgatgagt taaaattcgt cgatccaact
     8581 gatgttcaag ctaaattgat tccagttgtt cgttcaggcc gcgatcttgt gggggaatcg
     8641 aaaactggtt ctggtaaaac ccacaccttt ttacttccta tttttgagaa gctagatgaa
     8701 agtagtgatg atgttcaagt tgttattact gcaccaagta gagagttggc aacacaaatt
     8761 tatcaggcaa ctaaacaaat tgctgagcat tcagagcaag aaatacgggt ggttaactat
     8821 gttggtggta ctgacaaatt acgtcaaatt gaaaagttga aagtaagcca acctcatatt
     8881 gtcattggaa ccccaggacg tatttatgat ttggtgaagt caggtgattt agttattcat
     8941 aaggcgcata cttttgtggt tgatgaagca gatatgactc tggatatggg attccttgat
     9001 accgttgata agattgcagg gtctttaccc aaggatgttc aaattttagt cttttcagca
     9061 acgattccac aaaaattaca gccattttta aagaaatatt tgaccaatcc agtaatggaa
     9121 aaaatcaaaa cagcaacagt tatcgctgat accatcgata actggctttt gtccactaaa
     9181 ggacgtgata aaaatgcaca aattttggaa ctctctaaac taatgcagcc ttaccttgca
     9241 atgatatttg ttaatacgaa agagcgtgca gacgaattac actcgtatct ttctagtaat
     9301 ggcttaaaag tagctaaaat tcatggtggg attgcacctc gtgaacgtaa acgtattatg
     9361 aatcaggtta aaaacctcga atttgaatat atcgttgcaa cagatttagc agcgcgtggt
     9421 attgatattg aaggtgttag ccatgttatc aatgatgcca tccctcaaga cctatctttc
     9481 tttgtccacc gtgtcggtcg cacaggccgt aatggcttat ctggtactgc tataacttta
     9541 tatcagccaa gtgacgattc tgatattcgt gaacttgaaa aactaggtat taattttatt
     9601 cctaaagtca ttaaaaacgg tgaatttcaa gatacatacg accgtgatcg tcgtaataac
     9661 cgtgaaaagt cctatcaaaa attagataca gaaatgattg gacttgtgaa aaagaaaaag
     9721 aaaaaaataa aaccaggtta caagaaaaaa attcaatgga aagttgaaga aaagcgtcgc
     9781 aaagagcgta gagcaagtaa tcgtgctaag gggcgtgcgg agcgcaaagc taaaaaacaa
     9841 agtttttaaa attattaaag tctgaaatca gttcgtttca ggctttttta ttgatataaa
     9901 atctttatca cctgataagg aaatactatt atacagatag attttagagt gttagactag
     9961 taatatgata ttttaggaga attcttatgt ttaaaaaaat aatattatct attgcaacta
    10021 tagccgcaac cgccagccta gcagtatcag ttcaggcgtc agagaaagta gaacttaaag
    10081 tagctacaga ttctgacacg gcaccattta cttatcaaaa agacgggaaa ttcaaaggtt
    10141 atgacgttga tgttgtcaaa gctgttttta aaggtagtaa gtacaaagta accttcaaga
    10201 cagttccttt tgatactatt tcaacaggta ttgatgcagg gaaatttgat ttatcagcta
    10261 atgatttttc atataataaa gaaagagcag aaaaatatct cttctcagat cctatatccc
    10321 gttcaaatta tgccgtagta gggaagaagg ggagccatta caaatcatta agtgacctct
    10381 ctggaaaatc aacagaagtt ttatctggcg ttaactatgc acaggttcta gaaaattgga
    10441 ataaaaatca tcctaataaa aaaccaataa aaatcaaata tgtttctggg acaactggtg
    10501 ttactagcag attaaaaaat attgagagtg gaaaaattga ctttatccta tatgatgcca
    10561 tttcatctga ctatattgta aaagatcaat cattaaactt aagcgtttct cctttgaaag
    10621 gtaaaattgg taataataag gatggattag aatacctcct tttaccaaaa gataaaaaag
    10681 gtaaaactct acagaaattt ataaataagc gtattaaagt tttgaaagaa gatggtactt
    10741 tggcacgttt aagtaaacaa tatttcggtg gagattacgt ttcaaacatt gataaataaa
    10801 tcagtgagac tataagtttt atctttttac atgtgagagt ttgcgtgata gaataacgaa
    10861 gataagaaag tttagaaaag gaaagtagaa ggaatgaatc aatttatctt gacgggtgga
    10921 tggtcttggt ataataattt ggttagtcag gttccagcgg ggaaattatt tagttggaag
    10981 gctgttttag atgcaatacc ctcaatttta gagcgattac ccataacgct attattgaca
    11041 gtagcaggag ccttatttgg actcatttta gctttgattt ttgcagtagt gaaaattaac
    11101 cgtgtcaaaa ttttatatcc gattcaagct ttatttgtta gttttttacg tgggactcca
    11161 atacttgttc aattaatgct gagttattat ggaattccac ttttcttgaa atttttaaac
    11221 caaaaatatg gttttgattg gaacattaat gctataccgg cttctgtatt tgctattact
    11281 gcatttgctt ttaatgaagc ggcttataca agtgaaacta tacgagcggc gattttgtcg
    11341 gttgatcaag gtgaaattga ggcagcacgt agtttaggga tgacctcagc gcaagtttat
    11401 cgtcgtgtta ttattccaaa cgcagcagta gtcgcaacac ctactttgat aaacacgtta
    11461 attggtctaa ctaagggaac ctctttagct tttaatgctg gtattgttga aatgtttgcc
    11521 caagcacaaa ttatgggtgg tagcgattat cgctattttg aacgttatat ctctgtggca
    11581 cttgtttact gggcggttag tttcttaatt gaacaattgg gaaatgctat tgaacgaaaa
    11641 atggcaatta aggctccaca ccatttaact gatgagattc caggaggagt acgctaatga
    11701 taaaattgag acaattgact aaatcatttt ctggtcaaaa agtgttagac aaacttgatt
    11761 tagatattga aaaaggtcaa gttgttgctc tagtaggtgc ttcaggtgca ggaaaatcaa
    11821 cctttcttcg tagtatgaat tatttagaag agcctgatta tggaacaatt gaaattgatg
    11881 attttaaggt tgattttaaa tcaatcagta aagatgacat actaacatta cgccgtaagt
    11941 tagccatggt ttttcaacaa ttcaatctat ttgagcgtcg tacagctttg gataatgtaa
    12001 aagaaggttt gaaaattgtc aaaaagatgt cagatcaaga ggcaacacgg attgcaagag
    12061 acgaactggc aaaggtcgga cttgcagatc gtgaaaaata ttatccaaga catttatcag
    12121 gtggacagaa acaacgtgtt gctttggcac gcgcattagc catgaaacct gatgttcttc
    12181 ttttagacga accgacatca gcacttgatc cagaattagt tggagaagtt gaaaaatcta
    12241 ttgcagatgc tgctaagcag gggcaaacca tggtgcttgt tagtcatgat atgaattttg
    12301 tttaccaagt agcagataaa gttttatttt tggaaaaagg tcgtatttta gagtctggga
    12361 cgcctgaaca attatttaac catcctttag aagaacgtac aaaagaattc ttcgctagtt
    12421 ataacaaatc gtacctataa gctaaaatat tttgatataa tgaaggttag aatgtctaat
    12481 cttcattttt aattattctt gagttaggct aagtatataa gaaagctgcg atatgttttg
    12541 acaatcgctt gttggataga gatgttttta gaaatagcaa aagtttattt acaaggttta
    12601 gggcttgcca gcataactgt tattttagtt ggtatatttt gggtcttttg gcgtgccaag
    12661 aaaaacatag ataaaacagc tagagaacgc caatctgttc tttatgatgt ccttctgata
    12721 gctcttatga cagctccaat acttgccttt gcatttgtag ctatcatttt aatgataaaa
    12781 gcttagtcag attagttgga aatatagacg ctttgctata gaataaaacc ataagattca
    12841 aaggagaaat agtaatgtat gatactttaa taataggttc aggtcctggt ggtatgacag
    12901 cagcgcttta cgctgcacgt tcaaacctta aagttgggtt aattgaacaa ggtgctccag
    12961 gtggacagat gaataatact gctgaaattg agaactatcc aggctacgat catatctcag
    13021 gtccagaatt atcaatgaag atgtatgagc cgttggaaaa atttgaagta gagcatattt
    13081 atggtatagt tcaaagagtt gagaacgatg gtgatgtaaa gcgtgttatc accgaagatg
    13141 aatcgtatga agccaaaaca gtaatccttg ctaccggagc taaaaacagc cttttaggag
    13201 ttccgggtga agaggaatat actagtcgtg gcgtttctta ctgtgcggtt tgtgatggtg
    13261 catttttccg tgatcaggat cttttagttg tgggtggcgg tgattctgct gttgaagaag
    13321 ctgtcttctt aactcaattt gctaaaagtg ttactattat tcaccgacgt gatcaattga
    13381 gagctcaaaa agtacttcaa gaccgagctt ttgctaacga aaaaataaaa tttgtatggg
    13441 attcagtggt caaagagatt aaaggaaatg agattaaagt atctggtgta actgttgaaa
    13501 atctaaagac tggagaaatc tcagagatga ctttcggagg tgttttcatt tacgttggtt
    13561 tgaaaccaca ttctagcatg gtctcagaat taggtattac ggacgagact ggctgggtct
    13621 taactgatac aaatatgaaa acatcaattc caggactata tgctattggg gatgttcgtc
    13681 aaaaagatct acgtcagatt gcgactgctg ttggtgaagg tgctatcgct ggacagggtg
    13741 tttataacta tataactgag catttctaag aaatactaaa agtagaaaag actatcgaaa
    13801 aacgattagt ctttcttttt attttcatga aagtaaggtg ataaactgaa ctaccgagat
    13861 agaaatatgc tataatgtta gacatatagc cttatcctag gctcatgatt aaaaggagat
    13921 tgtctgatgt ataaagacga tagtttaaca ctacatactg atttatatca aattaatatg
    13981 atgcaagtct attttaataa aggtattcac aataaaaaag cggtgtttga agcttacttt
    14041 cgcaaggttc catttgaaaa tggttatgct gtttttgcag gtttagaacg tattgttcgt
    14101 tatttagaaa atctgagctt ttctgatagt gatttatcct atttagaaga gttaggttat
    14161 cctgaagaat ttttagatta cttaaaaaat ttaaaaatgg aactgactgt caaatcagct
    14221 aaggaaggtg accttgtatt tgctaatgaa ccattagttc aaattgaagg tcctttagca
    14281 caatgtcagt tagtagagac agcgatttta aatatcatca actaccagac acttgttgct
    14341 acaaaagcag ctcgtattcg ttctgttatt gaagatgaac cgctattaga gtttgggaca
    14401 cgacgtgctc aagaaatgga tgcagccatt tggggtacta gggcagcgat tattggtggt
    14461 gctaacgcaa cgagtaatgt tcgtgctggt aaaatcttta atattccagt atcaggaacg
    14521 catgcacatg ctttagttca aacttatggc gatgattacc aggcatttaa agcttacgca
    14581 gaaactcata aagattgcgt ctttttagtg gatacttatg atacccttcg tgtaggtgtg
    14641 cctaatgcta ttcgagttgc aaaagagatg ggcgaaaaga ttaatttctt gggagttcgc
    14701 cttgattcag gtgatttagc ttatttgtct aagaaagttc gtcagcagtt agatgacgcc
    14761 ggttttccta atgcaaagat ttatgcttcg aatgatctag atgaaaatac aatcctcaac
    14821 ctaaaaatgc aaaaagctaa aattgatgtt tggggtgtcg gaacaaaatt aattacagcc
    14881 tatgatcagc ctgcgctagg tgctgtttat aaaattgttt ctattgagac tgacgccggt
    14941 agtatgcgag atacaattaa actatcaaac aatgcggaaa aagtatcaac acctgggaaa
    15001 aagcaagttt ggcgtatcac cagtcgagct aaagggaaat ctgagggtga ttacatcaca
    15061 tttgcagaca ctgatgtgac acaattagat gaaattgaca tgtttcatcc aacttatact
    15121 tatatcaata aaacagtccg tgattttgac gcagtaccct tactggtaga tatctttgac
    15181 aaagggaaat tagtttatca attaccaagc cttcaagaaa ttcaagagta cggacgcaaa
    15241 gaatttgatc agctttggga tgagtataaa cgtgttctga atccacagga ttacccagtt
    15301 gatttggcac gcgatgtttg gcaaaataaa atggacctga ttgatcgtat ccgtaaagaa
    15361 gctttggcaa aaggagaagt aagatgactt tgcaagatca aattatcaaa gaactcggtg
    15421 taaaacctgt tattaatcca agtcaagaaa tccgtcgttc cgtagaattt ttaaaagact
    15481 atctattaaa acatagtttt ctaaagacct atgtactggg tatttcaggg gggcaagatt
    15541 caactttagc aggacgtcta gctcaactag cagttgaaga gttgcgtgct gatacagggg
    15601 aaaactatca atttatagct attcgtttac catatggtat tcaggccgat gaagaagatg
    15661 cacaaaaagc attagatttt atcaagccag atattgcttt gaccattaat attaaagaag
    15721 ctgttgatgg ccaagttaga gctcttaatg cagcaggagt agagattaca gactttaata
    15781 aaggaaatat taaagctcgt caaagaatga ttagtcaata tgcagttgca ggtcaatatg
    15841 ctggagcggt tattggtact gatcatgctg cagaaaatat tacaggtttt ttcaccaaat
    15901 ttggtgatgg aggtgcggac ttattacctc tctttagact taataaaagt cagggaaaac
    15961 agttattagc tgaacttggt gctgataaag acttgtacga aaagattcca accgcagatt
    16021 tagaagaaaa taaaccagga atcgctgatg aaatagcact tggagtaacc taccaagaaa
    16081 ttgatgctta tcttgaaggg aaggtagttt cggacaagtc tcgaggtatc attgaaaatt
    16141 ggtggtataa gggtcaacat aaacgtcatc ttccaatcac aatttttgat gatttctgga
    16201 aataagaaaa atagctagaa tattctagct atttttgttt ctctaataaa ttactttcag
    16261 acttgcagaa atattcagaa tgtttataat agtagctaag tataaaagga ggaatttatg
    16321 tcagaattaa ctcaaacatt tacagataag ttattagctg attaccaagc aaatacaaaa
    16381 tttagcgcta ttgaaaatgc agtaactcac aatggtttat tgaagtctct agagactcgt
    16441 caaagtgaga ttgagaacga ctatgtcttt tcaattgatt tgaccaaaga tgaagtctct
    16501 aatcagaaac aatcaggacg ttgctggatg tttgctgcac tgaacacatt tcgtcataaa
    16561 ctgatttcag attttaaact cgagaacttt gaattatctc aggcacatac atttttctgg
    16621 gataagtatg aaaaatcaaa ttggtttatg gaacaaatta ttgcgactgc aaatcaggaa
    16681 ttgagtagtc gtaaggtaaa attcctatta gatgtacctc aacaagatgg cggacaatgg
    16741 gacatggtgg ttgctctgtt tgagaaatat ggtgtcgttc ctaaaacagt ttatcctgaa
    16801 tctgtttcat caagtgctag tcgtgaacta aatcaatatt tgaataaact tttacgccaa
    16861 gatgcacaga ttctacgaga attaatagcg cagggagcag atggggcaac tgttcaaaat
    16921 aaaaaggaag aattgcttca agaaattttt aactttttag caatgaacct tggcttgcca
    16981 ccgcaatctt ttgattttgc ttatcgtgat aaagacaatc attatcagtc tgataaaaat
    17041 attacaccga aagcatttta tcaaaaatat gttaatcttg acatatcaga ttatgtttct
    17101 attatcaatg ctccaactgt agataagcct tatggacaat cctatacagt tgagatgctt
    17161 ggaaatgttg tcggaggccc agctgttaaa tatttaaacc ttgatatgaa acggtttaaa
    17221 gagttagcga ttgcgcaaat gaaatcagga gaaactgtgt ggtttggttc agacgtaggt
    17281 caagtatcaa atcgtcaaaa gggtatccta gcaactacga cttatgattt taattcatct
    17341 atggatatta aacttagcca agacaaagcg ggtcgcctag attatagtga aagtttaatg
    17401 acacatgcta tggtcttaac gggagtagac cttgatgaat ctggacaacc tttaaaatgg
    17461 aaagttgaga actcttgggg agagaaagtt ggtaaggatg gttattttgt agcttcagat
    17521 gcttggatgg atgaatatac ttaccaaata gtcgttcgta aagaattgct tactaaagaa
    17581 gaattagagg cttacaacgc agagccaatt actttagcgc cgtgggatcc gatgggagca
    17641 ctagctaatt agtaatagta attataaatt aaaaaactgg atatattatc cagttttttt
    17701 aattaccgtt aggtgctgta ttgttattac cattaccatg tccagttcct ggcacagttt
    17761 gattggaatt attaggggta gtagcattac cattattagg tgtttcgact tgtggggatg
    17821 atgaattggc atcatctgct gtattcttat cctctttttg tttctcagag gatgttgaac
    17881 tactgctttg acttgactgt ccagaatttt gatagatatt cttatagaca gaagaagagt
    17941 aagtgccggt tgtagttgtc ccattgatat acaagtagct tccacttcga tagagccctt
    18001 ctggcattgt ccaatctgca ctgtatccgc cagttaggta ggacatcatt gcacgataaa
    18061 cctcagtagc aatatccagt tggctaccat aaagtggtgt aaggcgattt ttataaccag
    18121 tccaaattgc cattgtgtac ttagaagtat agccgacaaa gttttcgtca ggagccattg
    18181 taccaacggc gctattgtag ataccagtag ttgcttcaat tttagctaac tcatcttctg
    18241 tatagttgga agtaccagtc ttaccagctt gtgcaacacc agggatagct gctttagtac
    18301 cagtaccaaa tgttagtact gttttcaaca tatccgtcat catgtaggca gtagtctctt
    18361 tcatcgcacg gctaccagac gctgcataag tatcattggt tccatcgcta aattcaattt
    18421 tattaacata ttgcggctta tagtaagttc cgccgtttgc aaaagccgag taagccgctg
    18481 ccattttctc actacttgca ccatattttg catcactgct attgttgtta cttgaaatag
    18541 catttgaata gttcatttct ggatagtata tgcctaattt ttcaaggaaa gattttgctt
    18601 cgtctaatcc agcggcttca agtgctctga cagcagggac gttacgtgat tgttgaatag
    18661 cggtctgcat actcatccaa cccatatatt gacgatccca gtcatatagt tgagtagaag
    18721 taccaggcca gtagtaaact gagtcgttta atgattgacc tgttgaatta tagacaccac
    18781 tatcaatagc aggtgcataa gctgagatag gtttcattgt agaaccccaa tcgcggtctg
    18841 ttaagacaga ttgatttgtc ccaaatgaaa tattttcatt ctgatgacgc ccgcctaatt
    18901 gtgcaatgac tttaccatta gtcgcatcca tgatggtaga tgctatttgt aattcattgt
    18961 ttggataagc gatgtaagta tcactgttgt agatgtcata tagttgtttt tgtgcatcag
    19021 tgttgatatt agtatacact tttagcccag cagtaaagat atctttacca gttttttgtt
    19081 taacttcact aataacttgt tttaagtagt tatccatata ttttggataa gtagattttt
    19141 gctttaattc ttttaagcca tcagttactg gagttgcaac agcttggtcg tattcctttt
    19201 tagaaatgtt tttatcttga tacatctgct gaagaacggt attacgtctt gtttgagcag
    19261 attctgggtt tttataaggg tcatattgtg taggtgcttg aggaatacca gcgagcaaag
    19321 caagttgtgc aatagataat tcctttaggt ctttaccaaa gtatgattta gctgttgttc
    19381 tcataccata attcccattt cccatataaa ctttattaat atagaaagta agaatttctt
    19441 ctttggtgta tttacgctcc atttgaagcg caagccaaac ttcctgtgat ttacgtttta
    19501 acgtttggtc agatttattg gtagaaaagt aagccagttt aatcaactgt tgatcaaggg
    19561 ttgaaccacc ttgcgtatta ctactaacaa ggttatgcca agctgcacct aaaatacgat
    19621 aaatatcgac acctctatgt ttaaagaaac gtttatcttc tatagaagtg atagcgttaa
    19681 ccaaatttag tggaatgcta tccgcactaa cactttcacg cttttctgag cctaaatcgg
    19741 caataagttt attattacca tcataaacca aactagagtt aacggatttt aaagcttgat
    19801 cggttaattt cggagaacga ctaacgtagt atgcaaatag gagcccacct attacaatag
    19861 ctaagataat aaatcccatt ataatgccaa aagcatactt caatagtttg ataacagatt
    19921 cctttttaat tgtaatcacc gcctaataat ttttcttcaa taatatctaa gtaagggact
    19981 tgaggaaagg cactctcctt tacgaaaaat ccattttttc tgatataatc aataggcatt
    20041 gatttattgc ctttatcaat ctgataaaat gaaattaact cattagcagg gagtagatag
    20101 gtttccttaa gtgtggaaaa atgaagcaag acaaagcaaa tccctttttg ctgtaataca
    20161 tttgccatgt gctctatttg gtgagcatga aaatttttca taggcatagc agttttctgc
    20221 cgggtttctt tggcttcaaa gtcaatatag taacctttgt aaacaccaga ataatcagta
    20281 gtagaagctt gtctaaaata agcttctaca atcttggcac gactacgttt aggataatct
    20341 accttgacga tttgtactgg cgttggtttc ttatgaatga cagctaattc gtgagacaaa
    20401 tagtagtcat tggttgcatt gatagcagct tcaaatgaca taccacggtt ggcaaaatcg
    20461 attttctttt tctttgattt tgtaactgtt gttttccgaa taagctgatg tggatagtta
    20521 accatttttc tccttgcatt acaaataaaa tgctaagata agcaaaacta ttataccata
    20581 aaattaggat ggacaacaaa tgaagtcaac tattcttgta acaggttaca aaaattttga
    20641 attagggatt ttccaggata aggatcctcg gattactatt ataaaaaagg ctattgataa
    20701 ggattttaga cgcttcttgg aaaatggagc agactggttt atttttatgg gaaatttagg
    20761 gtttgagtac tgggctttgg aagttgctct tgatttacaa aaagaatatg attttcaaat
    20821 tgcgacaatt tttacctttg agaaccatgg ccaaaattgg aatgaggcta ataaagctaa
    20881 gttggcactc tttaagcaag ttgactttgt taaatatact ttcccaagct atgaaaaccc
    20941 gggtcaattt aaacaataca atcattttct cattaacaac acacaaggtg catatctttt
    21001 ttatgattct gaaaatgaaa ctaatttaaa atttttgcta gaaatgatgg aaaaaaagga
    21061 agcttatgat atttctttcc taacttttga tcgcttaaat gaaatatatg aggaatgatc
    21121 gttttttcgg atatgagaac gttttacgct tgatttttcc atttaattta ggtattattt
    21181 actttgatat aaaggatagg acttatatta aaacgatttg actggtttgg agaatagaat
    21241 ggcaagtatt atttatagcc caaaagatat ttttgagcaa gattttaaag ttagtatgcg
    21301 gggatatgac aagaaagaag tcgatgtatt tctggatgat gtcatcaaag attacgaaaa
    21361 ttatcttgag caaattgaga agctccaaat ggaaaataga cgtctacaac aagctttaga
    21421 caaaaaagaa agcgaagctt caaatgtaag aaattcaggg acagctatgt ataatcaaaa
    21481 accgattgcc caatctgcaa ccaattttga tattttaaaa cgcattagcc gccttgagaa
    21541 agaagttttt ggaagacaaa ttagagaata aggatttctc aaatatgcaa tttttggata
    21601 atcgcgtagt attgatatac tatgaggaaa gtccatgcta gcactggctg tgatgccagt
    21661 agtgtttgtg ctaggcgaaa aaataagcct agggagatag ctagctatct tacggcaagc
    21721 aaaagggcta agtctttgga tatgcctgaa tagctttgaa agtgccacag tgacgtagtt
    21781 tctagggaaa tctagaaagt ggaacgcggt aaacccctca agctagcaac ccaaactttg
    21841 gtaggggcat gggatagttg gaatgagaac aatctatcct gactgctttg cagtagacag
    21901 atgattatcg aaagaaacaa ttcctagttg tttctggaac aaaacatggc ttatagaaaa
    21961 ttgcataata ggttagtggc tgagataaca tctcagccaa tttttaactg tgatgaatac
    22021 gatagtgtaa aagaggaaaa atgaaagaat cattcaaatt aatagcaact gctgctgctg
    22081 gtttagaagc cattgttggt agagaaatca gaaacctagg aattgactgc caagttgaga
    22141 acggtagagt ccgtttccac ggagacatca aaacaatcat tgaaacgaac ctatggctac
    22201 gagctgccga tcgtataaag attattgtag gagagtttcc agcacgtact tttgaggaac
    22261 tttttcaggg tgtatatggc cttgactggg aaaattattt accgttaggg gcaaaatttc
    22321 ctattgctaa agcaaagtgt gtcaagtcaa aattgcacaa tgaacctagt gtacaagcca
    22381 tttctaaaaa agctgttgct aagaaacttc aaaaagtctt tcaccgtcct gaaggagttc
    22441 ccttacaaga aaatggagct gaatttaaaa tagaagtttc tattttaaaa gataaagcga
    22501 ctgtcatgat tgatacgact ggatcaagtc tcttcaaacg tggttaccga gcagaaaaag
    22561 gtggtgcgcc aattaaagaa aacatggcag cagctattat tcaattatct aattggttcc
    22621 ctgataagcc attgattgat ccaacttgtg gttcaggtac tttttgtatt gaagccgcta
    22681 tgattggaat gaatatagct cctggtttta atcgtgattt tgcctttgag gcgtggccat
    22741 gggtagatca aagtcaagta caaaaggttc gcgatgaagc tgagagtaaa gcaaactatg
    22801 atattgattt agatatttca ggttttgatt tagacggtcg tatggtagaa attgctagaa
    22861 aaaatgcaga agaagcagga cttggagatg ttatcaaatt gaaacaaatg cgtcttcaag
    22921 atttaaaaac agataaaatt aacggtgtta ttatctccaa cccaccatat ggtgaacgac
    22981 tcctggatga caaagcagtg gacattttgt ataatgagat gggacaaaca tttgcgcctt
    23041 taaaaacttg gagcaagttt atcctaacta gtgacgaagg atttgaaaaa aaatacggct
    23101 cacaagctga taaaaaacgt aagctttaca atggtacttt aaaagttgat ttgtaccaat
    23161 attacggtga acgtgtgaga cgtcaagtaa aataacgggg agaactcatg tcagaagatc
    23221 aaaaacaccc attttttgaa ccagaaaaag aaactgatgg tcttgaattt aaagatgcta
    23281 aagaaatgac agtagaagag gctgttagaa aagattctga aattaaggct ggtattaccg
    23341 aagaagatag tattcttgat aaatacatca aacaacatcg tgatgaagta gcttcacaaa
    23401 agtttgagac aaaatcaagc gattttgcaa accttgatac ggcatcttta gatgatttta
    23461 tcaagaaaca gcgcgaagaa ttatcagcga tgttagctgc agaagagctt tctaaaaagt
    23521 tggataattc agtctctcaa gaacaagata tagaagctaa tgctgtctca cctaaagaag
    23581 aatcttcaca agaacaagaa aatagtgtaa ctccagttcc tcctctgaat acggaagcag
    23641 aaccaactgc aacagaacca gattcgacta ttgctgattc agaggagtat aaatcctctt
    23701 ctaagaagcg tggaggtatt gtaggaacat tgattgctct tattttactt ctaatagtaa
    23761 caatctttgg ttacaactat ttcaagaata ataattcgac aaattctcaa actgcaacaa
    23821 gtcaaagttc tagttctaag gctacgacga cttcatcaga agaagataaa aaagctagtc
    23881 agaacttaga taatttcaac aaagcttatg ctaacttttt tgttgatgat aagaaaaccc
    23941 aactcaaaaa tagcgaattt gataaattgt cagaattaga aaagaaagtt gatgctttaa
    24001 aaggaactaa atactacggt aaagtaaaag ttaaatttga tagtctaaaa cgtcaaattg
    24061 atgctgttaa agctgttaac gacaagttta agtcgcctgc tgtagttgat ggtaagaaga
    24121 gtgaaaaatt agaagtaaaa gatggagcga attttgactc tttagatagt aaaactctta
    24181 atactggtaa tgctagtcta gatagtttac ttcactccat cgtttcaact ggtcgtaatc
    24241 aggtaaaaca atccgaagaa caggctaaaa aaactcaaga agaacagtct tcgtctaata
    24301 aggtttcaga cactcaaaca actgaacaac caaacgtaac taacggtcaa tcttcctcat
    24361 cagctgctac aataaataat caggcagctg gcactgcatc aggtaatttg gaacgtaacc
    24421 gtagccgagt accgtataat aatgctgcta ttgcggacac aggaaatcct gcctggatat
    24481 ttaacccagg agtattagag aaaatcgtgg ccacttcaca ggcgcgaggc tatttctctg
    24541 gaaataacta tattcttgaa cctgtaaata ttatcaatgg taatggttat tacaatatgt
    24601 ttaaattaga tggcacttat ttgttctcta tcaatgccaa gacaggttac tttgtaggta
    24661 acgctcctgg aagagctgat agtttagatt attaatataa agagcaaaac cagttttttg
    24721 tctacaccct aaaagttaga aaatcagtct aacgattagg gtgttttttg tattcgcatt
    24781 tagatgtgta cgactgctat gaggatatta gggaaaaatt aaaaggattt tgctctctat
    24841 gatacagaac aaaatccttt gtttaaatta ataagacaac ttttggaatt ccaaaagttg
    24901 acttttattt tttattagat cagacaatgt ggcgctcaaa agcattgtca gaaatccctt
    24961 gggaaagaat taatttcgcc cattcttgag cagagtggag actgtggtct ttataatttc
    25021 cacaagattc aatggttgtt ccaggaacgt cttcccaagt gaccccacca gcaatagcct
    25081 ctagactaga tttgatgact ttagcaattt cagtagcatc ctgttttccc cacataatca
    25141 tgtgaaaacc agttctacaa ccaaaaggag aacaatcaat caaaccatta atttgttggc
    25201 ggattaactt cgccagaagg tgctcgatag tatgtaaacc agcagtatca atagcatttt
    25261 cattaggctg aataaggcgg atatcaaagt tagtaatgat gtctccgacc ggtccaactt
    25321 cttctgaaat taaacgaacg tagggtgctt taacgatggt gtgatctaat tcaaaactct
    25381 ctacgacaac ttcttttgtc ataagttttc tccttttgta ttacattcta gagactaaag
    25441 gagatgttga aaatttctcc taatc
//
LOCUS       DATRNX010000018        24038 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRNX010000018 DATRNX010000000
VERSION     DATRNX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..475
                     /locus_tag="VBC81_000595"
     CDS             <1..475
                     /locus_tag="VBC81_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911655.1"
                     /translation="KGISTSVKRRSFLDVILKLISKTPSRLWTNLFVRAFLRSSDYLG
                     LTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFDYQYMNQLYPVRLN
                     AKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYYIPYKIKKMID"
     gene            533..1333
                     /locus_tag="VBC81_000596"
     CDS             533..1333
                     /locus_tag="VBC81_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6911656.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            1323..1958
                     /gene="trmB"
                     /locus_tag="VBC81_000597"
     CDS             1323..1958
                     /gene="trmB"
                     /locus_tag="VBC81_000597"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6911657.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            2002..2089
                     /locus_tag="VBC81_000598"
     tRNA            2002..2089
                     /locus_tag="VBC81_000598"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2036..2038,aa:Ser,seq:gga)
     gene            2447..2923
                     /gene="rimP"
                     /locus_tag="VBC81_000599"
     CDS             2447..2923
                     /gene="rimP"
                     /locus_tag="VBC81_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6911658.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            2950..4110
                     /gene="nusA"
                     /locus_tag="VBC81_000600"
     CDS             2950..4110
                     /gene="nusA"
                     /locus_tag="VBC81_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6911659.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            4132..4428
                     /locus_tag="VBC81_000601"
     CDS             4132..4428
                     /locus_tag="VBC81_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6911660.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            4421..4723
                     /locus_tag="VBC81_000602"
     CDS             4421..4723
                     /locus_tag="VBC81_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6911661.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            4743..7526
                     /gene="infB"
                     /locus_tag="VBC81_000603"
     CDS             4743..7526
                     /gene="infB"
                     /locus_tag="VBC81_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6911662.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            7616..7984
                     /gene="rbfA"
                     /locus_tag="VBC81_000604"
     CDS             7616..7984
                     /gene="rbfA"
                     /locus_tag="VBC81_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6911663.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(8056..9060)
                     /locus_tag="VBC81_000605"
     CDS             complement(8056..9060)
                     /locus_tag="VBC81_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6911664.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            9223..9639
                     /locus_tag="VBC81_000606"
     CDS             9223..9639
                     /locus_tag="VBC81_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6911665.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            9652..11886
                     /locus_tag="VBC81_000607"
     CDS             9652..11886
                     /locus_tag="VBC81_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6911666.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            11927..12133
                     /locus_tag="VBC81_000608"
     CDS             11927..12133
                     /locus_tag="VBC81_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6911667.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            12243..12857
                     /locus_tag="VBC81_000609"
     CDS             12243..12857
                     /locus_tag="VBC81_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6911668.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            12872..13684
                     /locus_tag="VBC81_000610"
     CDS             12872..13684
                     /locus_tag="VBC81_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6911669.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            13797..16439
                     /gene="polA"
                     /locus_tag="VBC81_000611"
     CDS             13797..16439
                     /gene="polA"
                     /locus_tag="VBC81_000611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6911670.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANVMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            16469..16909
                     /locus_tag="VBC81_000612"
     CDS             16469..16909
                     /locus_tag="VBC81_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6911671.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            16991..17470
                     /gene="perR"
                     /locus_tag="VBC81_000613"
     CDS             16991..17470
                     /gene="perR"
                     /locus_tag="VBC81_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6911672.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            17623..19188
                     /locus_tag="VBC81_000614"
     CDS             17623..19188
                     /locus_tag="VBC81_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO6911673.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            19301..19987
                     /locus_tag="VBC81_000615"
     CDS             19301..19987
                     /locus_tag="VBC81_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911674.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            19989..21026
                     /locus_tag="VBC81_000616"
     CDS             19989..21026
                     /locus_tag="VBC81_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6911675.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(21040..21780)
                     /locus_tag="VBC81_000617"
     CDS             complement(21040..21780)
                     /locus_tag="VBC81_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6911676.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            21967..23109
                     /gene="tgt"
                     /locus_tag="VBC81_000618"
     CDS             21967..23109
                     /gene="tgt"
                     /locus_tag="VBC81_000618"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6911677.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            23216..23527
                     /locus_tag="VBC81_000619"
     CDS             23216..23527
                     /locus_tag="VBC81_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911678.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            23534..>24038
                     /locus_tag="VBC81_000620"
     CDS             23534..>24038
                     /locus_tag="VBC81_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6911679.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAV"
BASE COUNT         8108 a         3710 c         4873 g         7347 t
ORIGIN      
        1 taagggaatt tcgacaagtg taaaacgtcg ttcattttta gatgttattt taaaacttat
       61 ttctaaaacg ccatcgcgtt tatggactaa cttatttgtg agggcatttc taagaagctc
      121 agattatctt ggtttaacca taaggttggt aactttaaat atcttatcag tcatctttgt
      181 taacgaaacc tacctcgccc ttgctctggc atttgtattt aactacttat tattgttcca
      241 attattagct ttaggtcacc attttgatta tcaatacatg aatcagctct atccagttag
      301 attaaatgct aaagcaagtc agctaaaagg ttttctcaga gtgttatcat atgctgtgac
      361 agtaattgat atcatactta taagagaatt gaaacctgtt atattattga tagttttgat
      421 gttaattgtg acggaatatt acatccctta caaaataaag aagatgattg actaaagcct
      481 ataaaacaag taaaataaga ggaaacgact aaatgaaaag gagggactgc tggtgacaat
      541 atcaaatcaa gaattgacgc ttactccctt aagaggaaaa agtggcaaag cctatattgg
      601 tacttatcca aatggtgaac gtgtctttgt caaatataat acgacaccta ttttgccagc
      661 cttagctaag gaacagattg cgccacaact actgtgggca cgccgtacaa gtaatggcga
      721 tatgatgagt gctcaagaat ggttagatgg tcgaacattg actaaagaag atatgggtag
      781 taaacagatt attcatattt tactgcgcct gcacaagtct cgtcccttag taaatcaact
      841 gttacaatta gggtataaaa ttgaaaaccc ttatgatttg ttgatggatt gggaaaaaca
      901 aactcctatt caaatccgag aaaacactta tttgcaatct attgtgactg agttaaagcg
      961 tagtttacct gagtttcgaa cagaggttgc aacaatcgtt cacggagata tcaaacatag
     1021 caactgggtt attacaacaa gtggtttaat ttatttggtt gattgggatt ctgttcgttt
     1081 aacagatcgc atgtatgacg ttgcatatat tttaagtcat tatattccac aaaaacactg
     1141 gaaagattgg ctgtcttact acggttataa agataatgag aaagtctgga gcaaaattat
     1201 ttggtatggg caattttcat atttatcaca gataatcaaa tgttttgata aacgagatat
     1261 ggagcatgtc aatcaagaaa tttatgaatt acgaaagttt agagagttaa ttaaaaagta
     1321 taatgcgagt tagaaaacgt aagggtgcag aggaacactt agaaaataat ccgcactatg
     1381 ttatttcaaa tccagaagaa gccaaagggc gttggcatga gatttttgga aataataacc
     1441 ccattcacat agaagttggt tccggaaaag gggcttttat cactggtatg gcagagcaaa
     1501 atccagatat caattatatt ggaattgata ttcaattgtc agtgctttcc tatgctctgg
     1561 ataaagttct agatagcggt gctaagaata tcaaattact attagttgat ggttctagtc
     1621 tatcaaacta ctttgatact ggcgaagttg atttgatgta tcttaacttt agtgatccat
     1681 ggcctaagaa aaagcatgag aaacgtcgat tgacctataa gactttcttg gatacctata
     1741 aagacatact tcctgagcag ggggaaattc attttaaaac agataataga ggtttatttg
     1801 aatatagcct tgctagcttt tctcaatacg gtatgacttt aaaacaagtt tggttagatt
     1861 tacatgcaag tgattaccag caaaatatca tgactgagta cgaacgcaaa ttttcaaata
     1921 aaggacaggt catctatcgt gtagaagcac gattttagaa taagtaaata gtaagtgggt
     1981 agttatttgc tactccttta tggagaggtc gcatagtggc tgagtgcgca cgcttggaaa
     2041 gcgtgtagtc cctaatgggg gctcgggggt tcgaatcccc tcctctcctt taatcttgtt
     2101 aatgtatcag tgtttattaa gtgttcacct ttttcactcg tcaaaagtcc aagtttcgaa
     2161 aaatttcttt tattttatca tcgtttctct ttgagcttga agtattagca taaagttttc
     2221 taagctctta caaagaattg aggacaaatt ttaatttttt gatataagat tagtgtacgg
     2281 tgatgctaat ttaaagtaaa tgattgcaat aatcgtattt tgtgctataa tagtattaaa
     2341 taatacagaa aggggcggag ttttacttcg cctcttatgt gtgtgattca tagaatttat
     2401 agtcattgta agttctaaag caagttagaa agaagaggta gctgttatcg caaatcaaac
     2461 cattgttgat attgttactc aagtcgtaac tcctgctatt caagcaccgt ttgaattagt
     2521 agatgtagaa tacgaaaaaa tgggtggcga ctatgtgctc agtattttaa ttgataaacc
     2581 cggaggcatt acggttgaag atacagcaca attgacagac gttgtaagtc ccttacttga
     2641 cacaattcaa ccagatccat ttccagaaca gtatatgctt gaagtctcaa gtcctgggtt
     2701 ggaaaggcca cttaaaacag cagaagcatt atcaaatgct gttgggtctt atatcaatgt
     2761 aagtctgtat aaatccattg ataaagttaa aatatttgaa ggtgacttat tatcatttga
     2821 tggtgaaacc ttgacaattg attatatgga caaaacacgt cataaaactg tagatattcc
     2881 ctatcagacg gttgcaaaag cccgtcttgc ggttaaactt taagaaagga tgattcttgc
     2941 gaagcaagaa tggacactat gagcaaagaa atgctagaag ccttccgtat tttggaagaa
     3001 gaaaaacaca ttaataaaga agatattatt gatgcagtta ctgagtcact aaaatctgcc
     3061 tataagcgtc gttatggtca atcagaatca tgcgttattg aatttaacga gaagaaagca
     3121 gactttaccg tctatactgt tcgtgaagtt gttgatgaag tttttgatag ccgtttggaa
     3181 attagcctaa aagacgcact tgctattagc tcagcgtatg aacttggtga taagattcgc
     3241 tttgaggaat ctgttacaga atttggacgc gtggcagctc agtcagcaaa acaaactatt
     3301 atggaaaaaa tgcgtcgtca aatgcgtgaa gtaaccttca atgaatacaa acaacacgaa
     3361 ggtgaaatta tgactggtac tgttgaacga tttgaccaac gttttatcta tgtcaatctc
     3421 ggttcactcg aggcgcaatt atcgcatcaa gatcagattc ctggggagag ctttaaatct
     3481 cacgatatga ttgacgttta tgtctataaa gtggagaata atcctaaagg tgtcaacgtt
     3541 tttgttagcc gtagtcatcc agagtttatc aaacgcatta tggagcgaga aattccagaa
     3601 gtatttgatg gaactgttga gattatgagc gtctctcgtg aggctggaga tcgtactaaa
     3661 gttgctgtac gcagtcataa ttcaaacgtt gatgcgattg gaacaattgt tggacgcggt
     3721 ggaagtaaca ttaaaaaagt tatcagtaat ttccatccta aacgcgttga tgctaaaaca
     3781 ggattggaga tcccagttga agaaaacatt gatgttattc aatgggttga agatccagcg
     3841 gaattcattt acaatgctat tgcaccagcc gaagttgata tggtactttt tgatgatgaa
     3901 gatacaaaac gtgctactgt agttgttcca gatagcaagt tatcgctagc tattggtcgc
     3961 cgaggacaaa atgtgcgttt agcagctcat ttgactggct atcgtattga tattaaatct
     4021 gcaagtgaat acgaaaaaat ggaagcgcaa gaacttcaaa ctgaggaagt agctcaagaa
     4081 tctgaagtta tttcagataa ggcagaataa tataaatgag agaggtgttt tatggctaaa
     4141 acaaaaaaaa tacctttaag aaaatcagtt gtttctggtg aagtgatcga taagcgagat
     4201 cttttacgaa ttgttaaaaa taaagaagga caggttttta tagatcctac cggaaagcaa
     4261 aatggtcgag gtgcctacat aaaattggac aatgatgaag ctatcctagc gaaaaagaaa
     4321 cgagtgtttg atcgcagttt ttcaatggaa gtttctgatg aattctatga tgaactattg
     4381 gcatatgtcg accataaggt caaaagaaga gagttaggcc ttgaataatt cagaaaaagt
     4441 attaaatctc attgggttag cacagcgtgc ggggcgcctt attacaggtg aagaattggt
     4501 tattaaagca attcaaaatc aacaagtatc gctaatcttt ctagcaaatg atgctggccc
     4561 aaatttgacg aaaaaagtaa ctgataaaag taattactac aaaacagaag tctccacagt
     4621 gttttcaaca ctggaattaa gtgatgcgtt aggcaaacca agaaaagtgg ttgctgtcgc
     4681 agacgctgga ttttcaaaga aaatgaggac tcttatggca taacagaata ggaggacatc
     4741 aattgtcaaa gaaaagatta cacgaaatcg ctaaggaaat tggaaaaacg agtaaggaag
     4801 tggttgagca agctcaatcg cttggcttac ctgtaaaaag tcatgcttct agcgttgaag
     4861 aaaatgatgc tacacgtatt gttgagagtt ttagctcttc taaaactaaa gctccaacaa
     4921 attctgttca aactaatcaa ggtgtgaaaa cagaatctaa aactgttgaa accaaacaag
     4981 gcttgtccga tgataaacct tcaactcaac ctgtagctaa accgaaacca caaagtcgta
     5041 actttaaggc ggagcgcgag gctcgtgcta aagctgaagc ggagaagcgt caacataatg
     5101 gggatcaccg taagaataat cgccataatg atacacgctc tgacgacaga cgccatcaag
     5161 gtcaaaagcg ttcaaatggt aatcgtaatg ataaccgaca aggtcagcaa aataaccgta
     5221 ataaaaatga tgggcgttat gctgattata aacagaaacc ccaaacgcgt cctcagcaac
     5281 ctgcaggcaa tcgcattgac tttaaagctc gagcagcagc tttgaaagct gagcaaaatg
     5341 ctgaatactc acgtcatagt gagcagcgtt tccgtgaaga gcaagaagcc aaacgtcaag
     5401 cagctaagga gcaagaacta gctaaagcag cagcgttaaa agcgcaagaa gaagctcaaa
     5461 aagctaagga aaaacttgct tctaaacctg ttgctaaggt aaaagaaatt gtcaataaag
     5521 tagccgctac gccatcacag acagcagata gtcgtcgtaa aaaacaaact cgttctgata
     5581 agtctcgtca attttcaaat gaaaatgaag atggtcaaaa gcaaacaaaa aataagaaaa
     5641 actggaataa tcaaaaccaa gtgagaaatc aaagaaatag taactggaat cataataaga
     5701 aaaataaaaa aggtaagact aatggagctc caaaaccagt aacagagcgt aaattccatg
     5761 aattaccaaa agaatttgaa tatactgagg gcatgaccgt tgcggaaatt gcaaaacgta
     5821 ttaaacgcga accagcagaa attgttaaaa aactctttat gatgggagtt atggcaacgc
     5881 agaatcaatc attagatgga gacactattg agctattgat ggttgactac gggattgaag
     5941 cacatgctaa ggttgaagtt gatgaagctg atattgaacg cttctttgca gatgaggatt
     6001 accttaatcc tgataactta acggaacgtc cgcctgttgt tactatcatg ggacacgttg
     6061 accatggtaa aacaactctt cttgatactc tacgtaattc acgtgtcgct actggcgaag
     6121 ctggtggaat tactcaacat attggtgctt atcagattga agaagctggc aagaaaatta
     6181 ctttcttgga tacaccaggg catgcggcct ttacttctat gcgtgcgcgt ggtgcatcag
     6241 taactgatat tactatcctt atcgttgccg ctgatgatgg tgtcatgccg cagacagttg
     6301 aggcaattaa tcactctaaa gctgcgggtg tcccaattat tgttgctatc aacaaaattg
     6361 ataaacctgg tgcaaatcca gaacgcgtta tcagtgaatt agctgagcat ggtgtcatct
     6421 ctactgcatg gggtggtgag tctgaatttg ttgaaatttc agctaagttt ggtaaaaata
     6481 ttcaagaatt acttgagaca gttcttttag ttgctgaaat ggaagaatta aaagcagatg
     6541 cagatgttcg tgctattggt actgttattg aagcccgtct tgataaaggt aagggagccg
     6601 ttgcaacgct tcttgtccaa caaggtactt taaatgtaca agatccaatc gttgttggta
     6661 atacatttgg acgtgttcgt gcgatgacta atgatcttgg gcgtcgtgtc aaagttgcgg
     6721 gtccatctac accagtttct attactggtt tgaacgaagc tccaatggct ggtgatcatt
     6781 ttgctgttta tgcagatgag aaagctgcgc gtgctgcagg tgaagaacgt gcaaaacgtg
     6841 ctctcttaaa acaacgccaa aacacgcaac gtgttagcct cgagaactta tttgataccc
     6901 ttaaagctgg tgaagttaag tcagttaatg ttattatcaa agctgatgtg caaggttctg
     6961 tagaagcact tgcagcttct ctccttaaga ttgatgttga aggagtaaaa gttaatgttg
     7021 ttcactcagc agttggtgct attaatgagt ctgatgtaac tcttgctgaa gcatcaaatg
     7081 ctgttattat aggatttaac gtacgcccta ctccacaagc tcgtcaacaa gccgatgctg
     7141 atgatgtcga aattcgtcaa catagcatta tctataaagt tatcgaagaa gttgaagaag
     7201 cgatgaaagg taaacttgat cctgaatacc aagaaaaaat ccttggtgaa gccattattc
     7261 gtgagacctt taaagtttca aaagtgggaa ctattggtgg atttatggtt atcaatggta
     7321 aagttactcg tgattcaagt gttcgtgtta tccgtgatgg tgttgtcatt tttgatggta
     7381 aattagcaag tcttaaacac tataaagatg atgttaaaga agtcggcaat gctcaagaag
     7441 gtggtctaat gattgagaat tataatgatc tcaaagaaga tgacactatt gaagcataca
     7501 ttatggaaga aattaaacgt aaataacccc tatgagaaac ccaacttgta tctttagttg
     7561 gtagggaata caaaattatg gctaggctcg tcctagcttt caagttatga gcacaatgaa
     7621 gaaaggatta attatggcta atcatcgtat tgatcgcgtt ggaatggaaa ttaaacgtga
     7681 agtcaatgag attttgcgtc tccgagttaa tgatccacgt gtacaagatg tcacaattac
     7741 tgatgttcag atgttgggtg acctttcaat ggctaaagta ttttatacta ttcattcgac
     7801 acttgcttct gataatcaaa aagcacaaat tggtttagaa aaagcaactg gtactattaa
     7861 gcgtgaactt ggcaaaaacc ttactatgta taaaatacca gatttacaat ttgttaaaga
     7921 tgaatctatt gaatacggaa ataagattga tgaaatgttg cgaaatctag ataaaaaaga
     7981 ttaagaataa taaaaaataa accatgtcaa aatcatggtt tattttttat agctttagag
     8041 ttgagatagc tagtattaag gagttttatt gatgatatca atcatttgat actgtgctgt
     8101 tttagcttct tcaatagggt aaattggata aacatgattc attccctctt gtgcaatata
     8161 gttaacgggg tgattagctg cttggagttg tgcagcataa tcgcgaatat ctgggaagaa
     8221 tatttctcgt gttccagtaa ataacgtaat aggagcgagt ttagtagcag gggcattttt
     8281 aggactaaca tatgtatggt tagtattatt agaaccattt gcccaaattt cacctacacg
     8341 tgcgagaccc caagcagata ggataggatc ggtatcttcg tatttgggaa tttctggatg
     8401 tttcattgtc acatctagcc atggagataa gaggataata tttttaggtt gaggaatagc
     8461 ctcttgccct gattggtgag acaatgcatg cgctaatcct agtgctaatc ctccgccagc
     8521 tgaatcaccc atcaaagtga gattagcgtt tttttcatga aagtgtctat aaaggttaac
     8581 caatctaggg atagcataat cataggtata acgaggagtt tttggataaa ttggtaaaat
     8641 aatttttgca tcaagactag ttgagagcgt cttaagcatg ctgatatggt aaggcgttgg
     8701 attatttaag taagagccac cagctaaata gaaaatggtc ttttgattag ctttaccgtt
     8761 gtcattccaa gaatagactt gcatatcaaa aagtctctct tgaacaagcc ctccattaac
     8821 agagtttggg tctaagtaat aacccttatc agattgttca cggctagcat ttaagtactt
     8881 ttgaaatgag ctttcatctt gaactttata ataactttta tcgcgcttta gccaaaagta
     8941 ttgttcgata aaccaacttt tccatgaacg cccacttgct tttgcatgtg gtgtacaggc
     9001 gatggataaa atggtgataa tactaaatag tagcaatagt tttttgaagg acggtttcaa
     9061 ggtattctcc ttaatataat gttatttttg tgtaatatta ttataataaa accaacatct
     9121 ttgtcaatag aataaagaaa gatgagaaat gtttttcggt ttacaaatgt agatttattt
     9181 gctaaaattg gtttacaaac gtaaatcgaa agaggtgaga aaatgactat ttcaaacgca
     9241 gaatgggaaa ttatgagagt ggtttgggca cagcagaata caaccagtaa tgagatacta
     9301 gcagttttac tagagaagta tgattggact ccttcaactg tcaaaacctt attaagaagg
     9361 ctcttagata aaggctacgt gagcagagaa aaaatgggaa aaggatttag ctattcttct
     9421 ttaattgatg aagatttagc tatgatgtca gaggtagata gtgtattgca gaaagtttgc
     9481 cagaccaaac atgttgctat tgttagacac ttattggaaa gtataccaat gacagagaag
     9541 gatcgattaa atttgcaagc atcactagaa gctaagaaag gcaaaacact tgaacgtgta
     9601 gcttgtaatt gcattcctgg tcagtgtcaa tgccattaga aaggagaaaa tatggctaat
     9661 agaaaagaaa catttttgat tgatgggatg acttgtgcat cttgtgcttt aacgatagaa
     9721 aaagctgtca acaagttaga ccatgtggat agtgcagtag taaatttagc aacagagaaa
     9781 atgacagtta cgtttgatga tacaacctta tcgccaaacg tgattgaaga gtgtgtttct
     9841 gagactggat acgaagcatc gcttttcaaa gaggaaactt ctaaaagcca atctgaacgg
     9901 caccaattag caattgaaaa gatgtggcat cgcttttgga tgtcagcagt tgcaactata
     9961 cctcttctct atatttccat ggggccaatg attaacttat ggttaccatc atttttgatg
    10021 ccagataagg gacctctaaa ctatggtgtg atacaattat tgttaacact accggtgatg
    10081 tattttgggc gtatctttta ccaaaatggt tttaaagcac tttttaaaag gcatcccaat
    10141 atggattcat tagttgctat agccacaaca gcggctttca tttatagctt atatggatta
    10201 tacgaaatat tacagggtga catacactac gctcaccagc tttatttcga atctgttgca
    10261 gttattttaa cacttattac tctaggaaaa tattttgaaa tattatccaa agggcgtact
    10321 tcggcctcaa ttgaaaaatt attaacttta tcagcaaaag aagcaagagt tatcaaagat
    10381 ggtgaagact atatggttcc tttggataaa gttaaaatag gtgaaacaat tcttgtcaaa
    10441 cctggagaaa aaattcctct tgatggccat gttgtagcag gagaatcaag tattgatgaa
    10501 tccatgttaa caggtgaaag tattccagtt gaaaaaaagg ttggtagtaa ggtttacggt
    10561 gccagcatta atgggcaagg tagcttaacg attttcgttg aaaaagaagc tggcggttcc
    10621 cttctatcac aaattattaa cttagtagaa gttgcacagg caagtaaggc tccaattgct
    10681 aacttagcag acaaagtatc aggagtcttt gtcccttttg ttattgtcat tgcaatttta
    10741 tcaggtttga gttggtactt aattctgggg caaagttttg ccttctctct gaagattatg
    10801 attgcagtct tagtgattgc ttgtccatgt gctttagggt tagcaactcc tacagctatt
    10861 atggtggcta gtggtaaagc agctgagaat ggtattttat ttaaaggtgg tgaagtgttg
    10921 gaaaaggctc accacattga tacaatagtt tttgataaaa caggaacctt gaccaaaggg
    10981 aaaccagaag tagttgctat caaaacatat ggggtagata aagaggagtt tttaggtcaa
    11041 gtagcatcag tagaaaaact ttcaaatcat ccacttagcc aaacaattgt taacaaagca
    11101 aaagagaaag aacttccctt aagggaagtc atggctttta aaaatatttt gggttacggt
    11161 ttgagtgcaa ttattaatgg taaaactatg cttgtaggta atgctaactt aatgactaag
    11221 aacgatgtta atttagattt agcaaaggct gatatagaaa tagctcaaga agaagcacaa
    11281 actgtggttt acgtttccga aaatggagtg ttgagcggtt taataacttt aacggaccag
    11341 ctaaagacag atagccaaga aacagttaag cagttacaaa gattaggatt taatttagtt
    11401 ttacttactg gtgataataa ggctagtgca gatgccatag cacaaaaatt gggtatcacg
    11461 acagttgttt cagaagtttt acctgatcag aaagcaaacg ttattttaga acttaaagaa
    11521 aaaggcggac agattgcgat ggttggtgat ggcatcaatg atgctcctgc gctagctagc
    11581 agtgatgtag gtatctctat gagttctgga acagatattg ctattgaatc agcagatatc
    11641 gtgttgatga aacctgagtt aacagatctc ttaaaagcaa tgactatcag taaacaaacg
    11701 attcaaatta ttaaggaaaa tttattttgg gctttctttt ataacgtttt agcgattcca
    11761 gtggcaatgg gtgttttaca tctttttggt ggtcccttac tcaatcctat gttggcaggc
    11821 ttagctatgg cgtttagttc agtttcagta gttttaaacg cacttcgctt aaaagtctta
    11881 aaataataaa gataataaga ctgaattttt atgaagggag aagaatatga aacataccta
    11941 tcgagttagt ggcatgaaat gtgatggttg tgccaaaaca gtttctgata agttatcaag
    12001 tgtgatgggt gttgacgagg ttaatgttga tttgactaaa aatcaagttg tcgtaagtgg
    12061 aaaaacattc aaatgtcttt taaaacgttc tttaaaagat acaaagtatt cactagaaga
    12121 ggaaataaat taataactaa agaagtggtt acagctttta atttcttaat ttgagcagtt
    12181 tctcacttct tttttattct aaaaatgata aactagaata gaattagttg tgaggagtgc
    12241 agatgtcaat cgatatttgg aacattttaa gtttggttgg aaccgtagct tttgcaagtt
    12301 cgggagctat tgtggcgatt gaggaagaat ttgatgttct agggcttttt atcttaggtt
    12361 ttgtaacggc atttggtggt ggagctatta gaaatgtact aattggcttg cctattgaaa
    12421 cactttggag tcaaggaatt gctttttatg cagcagcagc agctattttg tttattatga
    12481 ttttccctaa cttgttgagt ggtaagggac gggatgccga agttgtatca gatgctattg
    12541 ggttagcagc gtttagtgtt caaggagcac tatatgcgac tcaatctcat caacctctta
    12601 gtgctgtcat tgtcgcagca gtactaactg gtgcaggtgg tggtattgtc agagatgttc
    12661 tggctggccg aaaacctggg gtactaagaa gtgaaatata tgcaggttgg tcaatattag
    12721 tggggatcat tttatatttt aaaattgcta aaacaacaac tgactattat ttgttagtgt
    12781 tagttgtaac tagcctaagg atgttggggt ataagaaaca atggcacttg ccagttgtca
    12841 gaccacgtaa gaaatgatag aggagaaaga gatgataaaa ttaattgcta ttgatatgga
    12901 tggaacgctt ttaaatgatg aaaagaaaat tccaaaggaa aatatccaag ctattaaaga
    12961 agcaactcaa gcaggtatta aaattgtact gtgtactggg cgaccgatgt caggtatact
    13021 tccttatttt aatgaactag gtttaacaaa agaggagtat attatcatga ataatggttg
    13081 tagcacttat agcacgaaag attggcaatt gattgattct gcaactttaa cacatgatga
    13141 actcatcttt cttgaggagg ttgttaaaga atttcccaac gtttgtttaa cgttaacagc
    13201 tgaaaataca ttttacgctg ttggtgagga agttcctgaa attgttgctt atgatgctga
    13261 tttagttttt acaaaagcaa agtcaataag tttagatgct cttcgcaatc aagaagaaat
    13321 tgtctttcaa gcaatgtaca tgggattaga tgcagatgta actgcttttc aagaagctgt
    13381 tgaagaagcc ttaatctcga aatttagtgg agttcgaagc caagactata tttatgagat
    13441 tatgcctcaa ggagtaacaa aagcaagagg cttaaaatcc ttgatagcaa aattaggact
    13501 ggatattaat caagttatgg ctattggtga tgctcctaac gatattgaat tattagattt
    13561 agttcccaat agcgttgcta tggggaatgc atcagatgag attaaatcta gatgtaaata
    13621 tataacggtg gataataacc aagccggggt tgctaaagct atttatgact atgctttacg
    13681 ttaaaataga tatagaataa taaaaggaac tttaaacact gtttgtttag ggttcttttg
    13741 tttaataaac taacaaaatt cagaagttta cagatagctt atgctataat agacctatga
    13801 caaacaagaa taaattactt ttaattgatg gctcatctgt agcctttcgt gctttttttg
    13861 ctctttataa tcaaattgac cgctttaaaa acaattctgg acttcatact aatgccatat
    13921 atggtttcca tttgatgttg aatcatattt taggtcgtgt ccagcctagt catattttag
    13981 tggcttttga tgctggaaaa actactttcc gtacggaaat gtatgcagat tataaaggtg
    14041 ggcgtgctaa aactccggat gaatttcgtg aacaatttcc ttatattcgt caacaattag
    14101 atgtcttagg catcaaacat tatgaattgg aacattatga agccgatgat atcattggta
    14161 ctcttgcaaa acaagccgaa gctagtaaag agcattttga tataacggtg gtttctgggg
    14221 ataaagattt aattcaatta acagacacta acacggtagt agaaatctct aaaaagggag
    14281 ttgctgagtt tgaagaattc acgccagctt accttatgga aaaaatggga attacgccga
    14341 gtcaatttat tgatttaaaa gctttgatgg gagataaatc agataatatt cctggggtta
    14401 ctaaaatcgg tgaaaaaact ggcttgaagt tgctatcaga atatggttcc ttagaaggga
    14461 tctatgaaaa tattgaggct atgaaacagt ctaagatgaa agaaaacctt atcaatgata
    14521 aggagcaagc tttcttatca aagaccctag ctacaatcaa catagcatca cctataacaa
    14581 tcggcttaga agatatcctt tatagtggac cgcaagatat aaaagccttg tctcaatttt
    14641 atgatgagat ggattttaaa cagtttaaag cagctctggg tgaggagaca agtcaggaag
    14701 attttgaggt tgactttact gaagtggagc aattaaaacc agaaatgttc tccgataatg
    14761 atttttatta ctttgagatg ttaggtgata actaccacgt agaagattta attgggatag
    14821 cttggggaaa tagcgataca atctacgcta ctagtgatgt atcgttattg caagaagctt
    14881 tatttaaaaa agctttatca aaacctatca aaacttatga ttttaaaaga agtaaggtcc
    14941 ttttaaatcg ttttaatatt gatttaccag agccagcctt tgatactaga ttggccaagt
    15001 accttttgtc tactactgag gataatctag tatcaacaat tgctaggtta tatacaaatc
    15061 tgccactgga tactgatgat gctgtttatg ggaaaggtgc taaacgcgct ataccagaaa
    15121 aaacacgatt tcttgagcat ctagctaaaa aagtaaaagt tttagtggat tctgaggcta
    15181 atgttatgca acagcttaaa gctaatgagc aagaagaatt gctgtttgaa atggagcaac
    15241 ctttagcgaa tgttttagct aaaatggaaa ttcgaggtat taaggttaag aaaaatacct
    15301 tgaatgaaat ggctattgaa aatcaaaaag tcatcgaaac cttaacacaa gagatctatg
    15361 aattggctgg gcaagagttt aatattaatt ctccaaaaca gttaggaaag ttgttgtttg
    15421 aaacgttagg tcttcctgta gaaatgacta aaaaaacaaa aacaggttat tcaactgcag
    15481 tagatgtatt agagcgctta gctccgattt ctcctcttgt aactaaaatt ttagagtacc
    15541 gtcaaatcac taaattgcaa tcaacctata ttattggttt acaagattat attttagaag
    15601 atggtaaaat tcatacacgt tatgttcaag atttgactca gacaggtcgt ctttcaagtt
    15661 ctgacccaaa ccttcaaaat ataccagtta gactagagca aggacggctt atccgaaagg
    15721 cttttgttcc atcagaagac aatgctgttt tattgagttc agattattct caaatagaat
    15781 tgcgcgtgtt agcacatatt tcaaaagacg aacatttgat cgctgccttt aaagagggag
    15841 cagatattca tacttcaaca gctatgcgtg tttttggtat cgaaaaacct gagaatgtca
    15901 ctcccaatga tcgtcgaaat gctaaggctg ttaactttgg aattgtttat ggtatttcag
    15961 attttggtct atcgcataat ttaggaatac ctcgtaaact agcgaagcaa tatattgaca
    16021 cctattttga gcgttatcct ggaataaaaa actatatgga aacagttgtt cgtgaagcaa
    16081 aagataaggg ttacgttgaa acacttttcc atcgtcgtcg ttcactacca gatattaatt
    16141 ctcgtaattt taacattcgc caatttgcag agcgcacagc aatcaattca ccaatccaag
    16201 gaagtgcagc tgatattctt aaaattgcaa tgataaattt agatagggta cttgataaag
    16261 gtggttataa atcaaaaatg ctgttgcaag ttcatgatga aattgtactt gaagtcccta
    16321 acgaggagat aggagcaatc aaggagttgg ttacaaaaac aatggagtca gcaattagtt
    16381 tatcggtacc tttgattgca gatgagaacg caggtgagac ttggtatgaa gcgaagtagt
    16441 ggacaacata cgtaaaggag tcattaatat ggtttatcat tttcaaaatc taagtgattt
    16501 tatgttaaaa aattatttaa caaaagcaaa gaccatcgca gttgtaggat tgtcagaccg
    16561 tcaagaaact gcagcttatc aagtatcaaa aataatgcaa gaagcaggtt atcaaattat
    16621 tccagttaat cctaaaaatg caggacagaa aatacttggg caaatgacat atgcctcact
    16681 taaggatgtc actgaacata ttgatattgt taatattttt cgacgtagcg agtatttacc
    16741 tgatattgca agagaatttt tagaagtaga cgctgatatt ttctgggctc agcttggact
    16801 tgaaagtcaa gaagcagaga caattctaaa gcaagcagga cacaaacaga ttgtcatgaa
    16861 taaatgtctg aaagttgaat accaaaaatt acactcagaa actaactaat taccgtgttt
    16921 aataggcaaa taatataaat tatgctataa taaccatgct aaattaaagt tcgtgctgga
    16981 aaggagtcct atggacagtc attcacatgg tcatagacct ctcgatgctt atgaaaacgt
    17041 cctagagcat ctccgtgaaa aacgtattcg aattactgag actcgaaaag ctattatttc
    17101 ctatatggtt aatagtcgtg agcatccgag tgcagaaaag atttacaatg atttattacc
    17161 agaatatcct aacatgagct tggcaacagt ttacaataac cttaaggtcc ttgtggatga
    17221 ggggtttgtg acagagctaa aactttgcaa ttatagtaca acttattatg attttatggg
    17281 gcatcagcac ctaaacattg cctgtgaaga ttgtgggaaa atcgttgatt ttgtcgatgt
    17341 tgacttattg gatatttcta gagaagcaca ccaacaaaca ggttttgaag tgactagagt
    17401 tcagttagta gcttatggta tctgtccgga gtgtcagagg aagcgtaagg ataaatataa
    17461 aaaagattga aacgtaattg ttcaatcttt ttttgtttct agactaagag aaaaagttta
    17521 agttttacta agtaataatt aagaaataat ttataattgc ttaaaaaatg atttattttt
    17581 tgttggtata ctctcattaa caaaaagaaa gaggaatccc ttatgaaaat atcacaatac
    17641 aataaatggt caattcgccg attgaaagtt ggagctgcat ctgtaatgat tgctagtgga
    17701 agcattgttg ctttagggca atctcatatt gtttcagcag atgagatgtc ccaacctaaa
    17761 acaaccatta cagccccaac agctaacact tctacaaatg tagaaagtag tactgataag
    17821 gcattatcaa aagttactac gatggagaca agttcagaaa tgcctaaaat gcaaaatatg
    17881 gcaaaagttg aaaaaacgag tgacaaacct atgatggtgg ctacctctgt tagaaaaatg
    17941 atggcaacgc cgacacctgt agcgatgacc aaaacgacta gtgtagatga ggtaaagaaa
    18001 tcaactgata ctgcttttaa acagacagta gacgttccgg ctcattatgt taatgcagca
    18061 aaagggaatg gcccatttct agcaggtgta aatcaaacga ttccatatga agcgtttggt
    18121 ggagacggca tgttaacgcg tttaatcctt aaagcttcag agggagcaaa atggtcagac
    18181 aatggtgttg ataaaaatag tccattatta ccattgaaag gcttaactaa aggtaaatat
    18241 ttctatcaag tttctttaaa tggtaatacg acaggtaaag aggggcaagc tctcttagat
    18301 caaattaaag ctaatgataa acatagctac caagccacaa ttagagttta tggtgctaaa
    18361 gatggtaaag ttgatttaaa aaacatgatt agccaaaaaa tggtaaccat taacattcct
    18421 cacattacaa ctgatatgga agtaaaaaat tctctaaaga tggcctttaa agaaaaagta
    18481 gacgtcccag caaaatatgt tagtgctgca aaagctaaag gtccattttt agcaggtgtt
    18541 aacgaaacga ttccatatga agcgtttggt ggagatggca tgttaacgcg tttaatcctt
    18601 aaagcttcag agggagcaaa atggtcagac aatggtgttg ataaaaatag tccattatta
    18661 ccgttaaaag atttaactaa gggtaaatac ttctaccaag tctctcttaa tggcaataca
    18721 gccggtaaaa aaggccaagc tctcttagat caaattaagg caaatggaag ccatacttat
    18781 caagcaacta ttactattta tggtacgaaa gatggcaaag ttgatatgaa tactatttta
    18841 ggacaaaaga cagtgatgat tcatattaat gtcgctaaaa aagatatgaa tagcacatca
    18901 atgatgatga aaaaagataa gatgacaatg cctatgaaga aagaaatgac ttcatctaaa
    18961 ataaataccg gtatgatgat gtctaacaac aagatgtcag ctaatatgca aatgtcatca
    19021 caagccaaat caaatgataa agctggtaaa aaaatgtcta tgatgtctaa aaatttacct
    19081 aatacaggtg aaacaaagca acaaaatgta ggtgtgctag gtatgcttag tctagccttt
    19141 gcgacaggtc taactgcgct aggccttaaa aaatctaaac aaagataggg atagtattta
    19201 attcagaaca ccaaatttgt ttaaaattat tgagggttat cgtggtaaaa tagtgttata
    19261 ctagaggtat agcactattt tgactcatta ggagagtaga atgacacaaa aattattatt
    19321 agttgacgat gaatttgaaa ttattgatat taatagacgt tatctagagc aagcaggata
    19381 tgaagtgtca gtagctgcgg atggcataga agcactcaaa gaggtagatg agaatcgttt
    19441 tgacctaatt attagtgata ttatgatgcc taaaatggat ggctatgatt ttattagtga
    19501 agttctagtt cgagaaccca accaaccttt tctctttata accgctaaag tatctgaacc
    19561 agataaaatt tactctttga gtatgggtgc agatgacttt atttccaagc ctttcagtcc
    19621 tagggaatta gttttacgtg ttaaaaatat tctaagacgt atctatggga atcatcaaca
    19681 atctgaagta ttgacgattg gtgatttggt tatcgaccaa aaacaacgtt tggtaatggt
    19741 agactgcaat acaatttcct taaccaataa atcatttgac ttgctttgga tattagcaaa
    19801 tcatttgaat cgtgtttttt caaaaacaga gctttatgaa cgtgtctggg gcgaagaatt
    19861 tttagatgac actaatactt tgaatgttca tatccatgcc ttacgcaatg accttgctaa
    19921 atttagtaca gacaataccc caactatcaa aacagtttgg ggattagggt ataaattaga
    19981 ggaataaaat gaaattaaaa tattatattg ttattggtta ccttatttct atgttaataa
    20041 ctgtagcagg tgttttcttt gggcttaatc atatgctgat tgagactcga ggtgtttact
    20101 acatcctttc agttacaatt attgcctgta tcgtaggtgg tatagttaat ttgttcttat
    20161 tatcaagtgt atttacatct ttgaaaaagt taaaacaaaa aatgaaggat atttctcaaa
    20221 gacgttttga tacaaaagca caaatttgtt caccacaaga attcaaggat ttagagacag
    20281 cctttaacca aatgtcttca gagttagagt caacgtttaa atctctaaac gagagtgaac
    20341 gcgaaaaaac aatgatgatt gcacaacttt cacatgatat caagactcca attacctcta
    20401 tacaatcaac agtagaaggt attttagatg gtattatatc tgaagaagaa gttaactatt
    20461 atctcaatac catttctcgt caaacaaacc gtcttaacca tctagttgaa gaattaagtt
    20521 ttattacatt agagactatg tcagacacgg ctgaacctca taaagaagaa acaatttatc
    20581 tggataaact actgattgat attttatctg aatttcaatt ggtgtttgaa aaagaaaatc
    20641 ggcaagtaat gattgatgtt gcgccagatg tttcgaaatt atctagtcaa tatgataaat
    20701 tatcacgaat tttattgaat ttgattagca atgcagtcaa atattctgat cctggaagtc
    20761 ccttgactat caaggcctat tcaaatcgtc aagatatcgt tatcgatatt attgaccaag
    20821 gctatgggat aaaagatgaa gatcttgctt ctatttttaa tcgtttttat cgtgtagaat
    20881 cttcacgaaa tatgaagaca ggaggacatg gcctaggtct ttatattgcg cgacaacttg
    20941 cacatcaact gaatggtgat attttggtcg aaagtcagta tcaaaaaggc agtaagttct
    21001 ctttagtact taaattacaa aaatagctag gaattattcc tagctatttt ttgtaatgtt
    21061 tctataaaag attaagcgcg cagtagtttg gtaaggaagg agatagatat agactaatcc
    21121 gaaactgaag attgtaagga aatgccaacc aataaaactt aagtctaaaa ggaagagtct
    21181 aagtttatta ccattcatta gttctcgact agcaactagt actttagatg gtccctcata
    21241 gtttccactt tctaattgag tgtaaaatac taaagttgag atactatagg agtaatattg
    21301 ttgaacgagt tttataactg ccttagtagt aattaggcta ctaacaacta ttaaaacagg
    21361 ggcaaggata aggttaccac tcaaaagtag cctaaaactt gaaatgatga ttagtagacc
    21421 aaaagtactt agtagataaa agaaagataa aattagtcct tttagaacaa gtactgagag
    21481 taatttccag aaaatatggc ttgaaaatac ataagtattg tccgagaatc taacgtttag
    21541 tttctgattt ttgacgacat ctaacattac aaaactgact gaaataataa aaagtgtgta
    21601 aaagaaataa actggaagag gtacaaaaaa atcaagtagt gtcatattat tatattgata
    21661 ataaaatgat atatataacg taataatact aagaagaatc gggagtaaaa aaagttgatt
    21721 cttaccttga agattgctta atatagtttt tgcctcattt ttaatttctg aattcgtcat
    21781 atctcctcca tacataaaag tacaattcat ttcattataa cacattcctt aagatgagca
    21841 tgacaagagt acattttttt gataaaatct taatggctaa aaaagaaatt aatacaaagt
    21901 attacattta gatattgatt agcactaaag cccatgactg tttttcatgg aagcaaggag
    21961 aaaacgatga ctgatcatcc aataaaatat cgattaatta aacaggaaaa acacacagga
    22021 gccagacttg gtgaaataat cacaccacat ggtacatttc cgacaccaat gtttatgcct
    22081 gttgggacac aagcaactgt taagacacaa tctcctgaag aactaaaaga aatgggttca
    22141 ggaattattc tatcaaatac ctaccattta tggttacgtc caggtgatga attgattgct
    22201 aaagctggag gtttacataa attcatgaac tgggatcagg ctattctaac agacagtgga
    22261 ggctttcagg tttattcttt ggctgatagt cgaaacatca ctgaagaagg agtaaccttt
    22321 aaaaaccatt taaatggtgc gaaaatgttt ttatcaccag aaaaagctat ttcaattcaa
    22381 aataaccttg gttctgatat tatgatgagt tttgatgagt gtccacaatt ttaccagcct
    22441 tatgattatg taaaaaaatc aattgagcga acaagccgtt gggcagaacg tggattaaat
    22501 gcccaccgcc gtcctcatga tcaaggacta tttggtattg ttcaaggggc agggtttgag
    22561 gatttacgtc gtcaatcagc acgagattta gtatcgatgg attttccagg ttattcaatt
    22621 ggagggcttg cggtcggaga aacacatgat gagatgaatg cagttcttga ttttactgtt
    22681 ccaatgcttc cgaatgataa gcctcgttat ttgatgggag ttggtgcacc agatagttta
    22741 atagacgccg ttatccgagg agtagatatg tttgactgcg tcttacctac tcgtattgct
    22801 cgaaatggga catgtatgac aagtcaaggt cgtttagttg ttaagaatgc taaatttgca
    22861 gaagatttta cgcctcttga tccaaattgt gattgctata cttgtaaaaa ttatacacgc
    22921 gcttatatcc gtcacttatt gaaagcagat gaaacttttg gtattcgttt gactagttac
    22981 cataatcttt atttccttgt caatcttatg aaagatgtca gacaagctat tatggatgat
    23041 aatcttctag agttccgtca agattttatg gagcgctatg gttatggcac gaataaccgt
    23101 aatttttaaa aaagttcctg gtaaaacagg gctttttttg atctgtttgt taaccgcgtt
    23161 ttaaaaaaaa gttgacaata gaaaaacttt tgtataatat gttataggag gaatcatgat
    23221 gcaagagtac tttggtattg gtcttgatag tagcagccgc tgttaccact atcatactaa
    23281 attggatatt gtcgctttaa aatgtgctgt ttgtcaaaaa tattatgctt gttataaatg
    23341 tcatgatgca ctcgaggagc attgttttgc agcaacaaaa tctgatgaaa tgtttcctgt
    23401 cttatgcggt tcttgtcgtc agatgttgac attaaaagag tataaaactg gtttttgtcc
    23461 ttattgtcgt atgcttttta atccaaattg tcaacgccat gattctattt atttttcgaa
    23521 ggagtaattt ttaatgtttc acacaaaaag cctaatccag atggctatga tggctacttt
    23581 attggtggta cttggtttca ttcctgctct accacttggt ttcattccgg tccctatcgt
    23641 cttacaaaac ctaggggtta tgatggctgg tgttattttg ggcggaagaa aagggagttt
    23701 atctattttg ctcttctttc ttgtcggttt agttattcct gtattctcag gtttacgttc
    23761 gactatacct gtattgacag gaccaacagc tggttatgtt attgcttggt tgtttgttcc
    23821 tttgcttatc tcatatggtg taaaattagt aaataaaaag aattttatag ctattttcct
    23881 tattgtatgg ctagcagggg tcctttttgt agatgtagca ggagcagttt ggctggcatc
    23941 ttatatgaac atcccattag ataaatcact tcttcaaaac ttagtatttc tcccaggaga
    24001 tacaataaaa gccattatag ctgctgttgt tgctgtta
//
LOCUS       DATRNX010000019        23723 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRNX010000019 DATRNX010000000
VERSION     DATRNX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23723
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            215..832
                     /locus_tag="VBC81_000621"
     CDS             215..832
                     /locus_tag="VBC81_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911680.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            1000..2439
                     /locus_tag="VBC81_000622"
     CDS             1000..2439
                     /locus_tag="VBC81_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6911681.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            2467..2745
                     /locus_tag="VBC81_000623"
     CDS             2467..2745
                     /locus_tag="VBC81_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6911682.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            2812..3297
                     /locus_tag="VBC81_000624"
     CDS             2812..3297
                     /locus_tag="VBC81_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6911683.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            3410..4075
                     /locus_tag="VBC81_000625"
     CDS             3410..4075
                     /locus_tag="VBC81_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6911684.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            4079..4942
                     /locus_tag="VBC81_000626"
     CDS             4079..4942
                     /locus_tag="VBC81_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6911685.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            4944..5660
                     /locus_tag="VBC81_000627"
     CDS             4944..5660
                     /locus_tag="VBC81_000627"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6911686.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            5671..6318
                     /gene="fsa"
                     /locus_tag="VBC81_000628"
     CDS             5671..6318
                     /gene="fsa"
                     /locus_tag="VBC81_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6911687.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            6481..7464
                     /locus_tag="VBC81_000629"
     CDS             6481..7464
                     /locus_tag="VBC81_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6911688.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVRYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            7699..8718
                     /locus_tag="VBC81_000630"
     CDS             7699..8718
                     /locus_tag="VBC81_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911689.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            8737..9693
                     /locus_tag="VBC81_000631"
     CDS             8737..9693
                     /locus_tag="VBC81_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6911690.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            9791..11242
                     /locus_tag="VBC81_000632"
     CDS             9791..11242
                     /locus_tag="VBC81_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6911691.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            11264..12253
                     /locus_tag="VBC81_000633"
     CDS             11264..12253
                     /locus_tag="VBC81_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6911692.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            12319..13836
                     /locus_tag="VBC81_000634"
     CDS             12319..13836
                     /locus_tag="VBC81_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6911693.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            13856..14617
                     /locus_tag="VBC81_000635"
     CDS             13856..14617
                     /locus_tag="VBC81_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6911694.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            14683..16362
                     /locus_tag="VBC81_000636"
     CDS             14683..16362
                     /locus_tag="VBC81_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6911695.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            16679..17770
                     /gene="ulaG"
                     /locus_tag="VBC81_000637"
     CDS             16679..17770
                     /gene="ulaG"
                     /locus_tag="VBC81_000637"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6911696.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            17860..20238
                     /locus_tag="VBC81_000638"
     CDS             17860..20238
                     /locus_tag="VBC81_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6911697.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            20615..21838
                     /locus_tag="VBC81_000639"
     CDS             20615..21838
                     /locus_tag="VBC81_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6911698.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            21857..23584
                     /locus_tag="VBC81_000640"
     CDS             21857..23584
                     /locus_tag="VBC81_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6911699.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         7487 a         3893 c         4771 g         7572 t
ORIGIN      
        1 tgtagtaaga agattgttct taaccgttta ttaatcaata tttcatttcc tcctacttat
       61 ttaccaataa gcttattata tcataatgta taatcaaaca ttcgttagta ctgattaaaa
      121 ggtttatgga ggggcggtta ctattgtttc attgaattag taactttaac gacgaaatgt
      181 tataatgtta ttagtttata aaagaggaga ttgtatgcgt aaatttttcc aagatgttat
      241 tgtagaggtg gcttattatt tagagattgc tttgtcagtt attctggcaa cagctattat
      301 tttctttagt ttagcactct ttcatgattt gtttagcatg ttgtctgttg atcaaaatgt
      361 tgataagttg ttccagactt tccttgctcg cgccatgaca atagcagtcg ggattgagtt
      421 gatcaaaatg ttttcaaaac cttcccctga tactttaatt gaagtgttat tgtttgcctt
      481 agctcgtcaa cttgtaattg aacatggtag tgtttcagat taccttatgg ggattgcttc
      541 agttgctatt ttgtttgctg ttcgtaaata tcttttcacc cattttgatg agtcaagcaa
      601 cattgtttta cgtgctaata acaaagtcaa aaaagctaat attttagcta gggttcaact
      661 accttgtcaa cccaatgaaa ctttaggaga cttacttgtt cgtcatttaa atgatgagga
      721 aaaggacgtt gctattggtt cagtagtcta tattaataac gttgcattgc gtgttgatag
      781 tatgcacgat ggtcttgtga cgcgtgtaga aatcattagg agtctacatt aaaaaatata
      841 aaaaatcatg tatcttagga tacatgattt ttttgtaaat taggttcagc acaattttgc
      901 acacttaagt gtgtacttct tggtcttttt taaaacgctt acaaaaaata taataaagat
      961 acaaaaaaac attacttaaa taaagagagg tctttagtta tggaaaactt cctagctccc
     1021 cttaattggt tctcgcaaaa tatcttgcag aatccagcct tcttcgtagg tctgttggta
     1081 ctcattgggt atcttttatt gaagaaacca cttcatgatg tctttgctgg ttttatcaaa
     1141 gcgaccgttg gatatttgat tttgaacgta ggtgcaggag gattggttaa tacattccgt
     1201 ccaatcttag ttgctcttgc taaaaaattt catttagaag ctgctgttat tgacccttac
     1261 tttggtttag catcagcaaa tgctaagtta gaaacaatgg gctttatcag tgttgcaaca
     1321 acagcactct tgattggttt tgggattaac attctcttgg ttgctcttcg taaagtaact
     1381 aaagtccgta ctctttttat tactggtcat atcatggtac aacaagctgc aacaatttca
     1441 gtatttgtac tgctcttgat tccacaactt cgtaatggtt ttggtgcatg ggctgttggt
     1501 atcatttgtg gtctatattg ggcggtaagt tctaatatga ccgttgaagc aactcaacgc
     1561 cttacaggtg gtggcggatt tgccatcgga catcaacaac aatttgcaat ttggtttgtt
     1621 gataaagttg cgccattttt cggtaaaaaa gaagaaaatc ttgataactt aaaattacca
     1681 acattcttaa atattttcca tgatacagtt gttgcatcag caacattgat gctagttttc
     1741 tttggaggaa tcttagctgt ccttggtcca gacattatgt ctaatgttaa attaatcgga
     1801 cctggtgcat ttgtaccaac taagcaagct ttcttcatgt acatccttca aacatcattg
     1861 acattctcag tttacctttt cattttgatg caaggtgtac gcatgttcgt tacagaattg
     1921 acaaatgcct tccaaggtat ctcaaacaaa cttcttccag gatcattccc agcggttgac
     1981 gttgcagctt catatggttt tggttcttca aatgcagttc tttcaggatt tgcttttggt
     2041 ttgattggtc aattaattac aatcgctcta cttgttgtct tcaaaaatcc aatcttgatt
     2101 atcacaggat tcgttccagt attctttgat aatgcggcaa tcgctgttta tgcagataaa
     2161 cgtggtggct ggaaagcagc agtagctcta tcatttatct caggtattat tcaggtggca
     2221 cttggtgctg ttgctgtagg tcttttagga cttgcaggtg gttatcatgg aaatatcgac
     2281 tttgaattcc catggttagc atttggttac atcttcaagt atttaggtat tgctggttac
     2341 gtgattgtat gtctcttctt cttagctatt ccacaacttc aatttatgaa atctaaagat
     2401 aaagaagcat actatcgtgg tgatgcgcct gcagaataat agaaatttat aacggaggaa
     2461 ataaaaatgg ttaaagttct tacagcatgc ggaaacggca tgggatcatc aatggttatc
     2521 aaaatgaaag ttgaaaatgc gcttcgtcaa ttaggcgtta gcaactttga gtcagcatct
     2581 tgctcagtcg gagaagcaaa aggtttagct gcgaactatg atatcgtggt tgcttcaaac
     2641 caccttatcc atgaattaga cggacgtaca aaaggtcatt tagtaggtct tgacaacctt
     2701 atggatgaca atgaaataaa aacaaaacta caagaaattt tgtaagttaa tagacaaagg
     2761 gagtgagaca aaagtttctc tcccatatct ttagactttt aaggagaaat catgaattta
     2821 aaacaagcct ttatcgaaaa tgactcaatt cgtcttaaat tatctgcaag cgattggaaa
     2881 gaagcaatca aattatcaat tgatccatta attgaaagtg gtgctgttga tgcagaatat
     2941 tacgatgcca ttattgaatc tacagaggaa tttggtccgt actacattct tatgccgggt
     3001 atggcgatgc ctcatgctcg tccagaagca ggcgtaaaac gtgatgcatt ttcattaatt
     3061 acattaacag agccagtagt ctttccagat ggcaaagaag tctcagtatt gttagcactt
     3121 gctgcaacaa gttcagctat tcatacttct gttgctatcc ctcaaattat tgcccttttt
     3181 gaattggaaa attcaattca gcgcttgacg gaatgccaag aagcaaaaga agtccttgca
     3241 atggtagagg aatcaaaaaa cagcccatat ttagaaggtt tagatttaga aagctaatca
     3301 ataaactgat attaatagaa aggataggac ttataaaaaa tctctgtgat aagaaactct
     3361 ttgccacaaa taaagtgagt atttttatca ggtccttaga ataaacaaca tgacaaaacg
     3421 tttaccaaat ttacaagttg cacttgacca ctcagattta caaggagcta tcaaagctgc
     3481 cgtatcagta ggacatgaag ttgatgttat tgaagctgga actgtttgcc ttcttcaagt
     3541 aggtagtgaa ttagttgagg tccttcgcag tctcttccct gacaaaataa ttgttgctga
     3601 tacaaaatgt gctgatgctg gtgggactgt tgctaaaaat aatgctgttc gcggtgcaga
     3661 ctggatgact tgtatctgtt gtgctactat tccaacaatg gaagcagcct taaaagctat
     3721 caaagaagaa cgcggcgatc gtggagaaat tcaaatcgaa ctatatggtg attggactta
     3781 tgagcaagcg caacaatggt tggatgcagg tatttcacaa gccatttatc accaatctcg
     3841 tgatgcgctt cttgctggcg aaacatgggg agaaaaagat cttaataagg ttaaaaaatt
     3901 gattgatatg ggcttccgag tttctgtaac tggtggttta tcaactgata ctcttcaatt
     3961 atttgaaggt gttgatgtct ttacttttat tgcgggtcgt ggtattacag aggcagatga
     4021 tccagccgca gcagcacgtg catttaagga tgaaattaaa cgtatttggg ggtaatgtat
     4081 gacacgtcca ataggaattt acgagaaggc aacaccaaaa cattttaact ggttagaacg
     4141 cctacaattt gctaaagaat tgggatttga ttttgtagaa ttatccattg atgagtcaga
     4201 tgaacgtttg gcacgtttgg agtggtcaaa agaagaacgc cttgagttgg tcaaagctat
     4261 ttttgagaca ggagttcgtg ttccaacaat tacatttagt ggtcatcgtc gtttcccaat
     4321 gggatctaat gatcctgaga aagaagcgcg tgcgatggat atgatgaaaa aatgtatcgt
     4381 ctttgctcaa gatattggta ttcgtaatat tcaattagca ggatatgatg tgtactatga
     4441 agaaaaatct ccagagacac gtgcacgctt tattaagaat cttcgtcaag cttgtacttg
     4501 ggccgaagaa gcacaagtta ttctgtctat tgagattatg gatgacccat ttatgaattc
     4561 cattgagaaa tatttggctg tggaaaaaga gattgattct ccttatctct ttgtctaccc
     4621 agatactgga aatgtatcag catggcataa tgatctttgg agtgaatttt ataatggaca
     4681 tcgctcaatt gcagcgcttc atattaaaga tacctatgct gttaccgaaa cgtctaaagg
     4741 tcaattccgt gatgttccat ttggtcaagg gtgtgttgat tgggaagaaa tgtttgctgt
     4801 cattaagaaa acaaattata atggtccatt cttgattgaa atgtggtcgg agaactgtga
     4861 gactgtagag gaaacacgtg cagccattaa agaggcgcaa gattttcttt acccattaat
     4921 ggagaaagca ggagtgaaat aatatggcaa aatcattgca agaaatgcgt gaacgcgtgt
     4981 gtgaagctaa taaatcacta ccagttcatg gtttagttaa atttacttgg ggtaacgttt
     5041 ctgaagttga ccgcgaagca ggattgattg ttatcaaacc ttcaggagtt gactatgacc
     5101 aattgacacc tgaaaatatg gttgtcacag acttagaggg aaatatagtg gagggggatt
     5161 tgaatccatc atctgatctt ccaactcatg ttcaacttta taaagcatgg ccagaagtcg
     5221 gtggtattgt tcatactcat tcaacagaag cagttggctg ggcacaagcg ggtcgcgata
     5281 ttccatttta tggaacaaca catgcagatt atttttatgg gccagtacca tgtgctaggt
     5341 cacttagtga agatgaagtt aacactgctt atgaaaaaga aacaggtagt gttattatag
     5401 aggaatttga acgtcggaat ttagatccaa tggctgttcc tggtattgtt gtacgtaatc
     5461 atggaccatt tacttggggg aaagatccag cacaagcagt ttatcactca gtcgttcttg
     5521 aagaagttgc caagatgaat cgtttcactg aacaaattaa tccacgtgtt gaaccagctc
     5581 caaaatatat tatggataaa cactatttac gtaagcatgg accaaatgct tattatggtc
     5641 aaaaaggaca agaacattaa ggagaataat atgaaatact ttttagatac tgccgatgtt
     5701 tctgaaattc gtcgtcttaa tcgtttagga atcgttgatg gtgttactac caatcctact
     5761 attatttcac gtgaggggcg tgactttaaa gaagttatta acgaaatttg ccaaatcgtt
     5821 gatggtccag ttagtgcaga agtgacaggt ttaacttgtg atgaaatggt taccgaagct
     5881 agagaaatcg ctaagtggtc accaaatgtt gttgttaaaa ttccaatgac tgaagaaggt
     5941 ttagcagcag tttcacaatt atcaaaagaa ggtataaaga caaatgtgac attaatattt
     6001 actgtggctc aagggctttc ggcaatgaaa gctggtgcaa cctttattag tccttttgtg
     6061 ggtcgtcttg aagatattgg aacagatgct tatgctttga ttcgtgacct tcgtcacatt
     6121 attgattttt atggtttcca atctgagatt attgctgcta gcattcgtgg attggcgcat
     6181 gtggagggcg tcgctaaatg cggtgcgcac attgccacta ttccagataa aacatttgca
     6241 tcactattta ctcacccgct tactgataaa gggattgaaa ctttcttaaa agattgggat
     6301 agtttcaaga agaagtagtg ttacgataaa gcaaactctt aattatacta gtgaaagaca
     6361 aactttgata atgggtttgt cttttttata atcttctcca ataagaacac ttattttcat
     6421 ttttcttatt aaatgatata atacggataa attaagtcta attagaataa ggaaatagag
     6481 atgtttgaga aggtaaccat tagtgatatt gcaaatttag taggtgtgtc aaaagctaca
     6541 gtttcctatt atctcaatgg gaactataag aaaatgtctc tgcaaacgaa agaaaaaatc
     6601 cgtttagcta ttaaggagac tggttatcaa ccaagtaaga ttgctcaaag tttagttaca
     6661 aaaaataccc gtactattgg ggtcgttatt gctgatatta ccaatccatt tatttcgtct
     6721 gtgatgaagg gaatacatga tacttgtcaa caatttggtt actcggttaa ctttactaac
     6781 tcggataatg atattgacat tgagttggaa aatttaaacc gtttaaatca gcaaaacgtt
     6841 tcaggaatta ttttagattc tgtagatcct aatcattcat ttatagaaac tttgagtaat
     6901 gatcgtcttg tcatggttga tagacaagct aaagatatta aggtagatac tgtagtatcc
     6961 gacaataaag aatcgactca aatatttctt gaaaaaatgc aagaaactgg gtatcatgac
     7021 atctattttg tcacttatcc cattgaaggg atttcaaccc gtgagcttcg ttacgaaggt
     7081 tttaaagaag tggtcagttc aaatcctgat aagttgatta ttatcactga agatggtagc
     7141 acacagagaa ttcttgatat tattgaacat tcagagcaaa aaccagggtt cttaatgatg
     7201 aacggtccaa ctcttttgaa ttttatgaaa aaattaaatc aatcaacggt cagataccct
     7261 gaggattatg gtcttgggag ttacgaagat ttagaatgga tgcaggtgct tacaccaaac
     7321 gtttcctgta taaaacagga ttcatatggc attggttgtc tggctgccca atgtttaatt
     7381 gaaaaaatta gtcaaggaaa cgaaccaact actgcaaggt tactagaagt aaaaaatcaa
     7441 atcgttattc gtcaatcttt ttaaaaatgt ttgcgagagc aagcattttt ttataaaaaa
     7501 gcaaaaaaca ctatgtaaac gcttgctttt ataaaaaatg atagtataat agaactataa
     7561 atgagttaac cgtttaactt aagaataggt aagcttttaa cgaaccatct ctaactctct
     7621 taactcaaat gtttttattt ttttgcacca ttagttaaac ggttaactca agttttatct
     7681 tcaccaaagg aggatagaat gaagattaat aaattattgt cagacagact tgaagcttgt
     7741 tattcagttt gtcgtttgca aaatcaagac tatgattttt tattagtagc ttcagaggtt
     7801 gatcaagcct gctttgctta tgatttgaat agcgatttag caaaaattgt tgtttgggaa
     7861 gatgtgggag gtaccatgag tattattcca attcccggaa ccatggattt tttggcaacc
     7921 cagaaatttt acccagggtt taaggccaaa gattgtcagg ttgtttatgc aagttttcaa
     7981 ggagatcatt gggacatttg taacgcattt gattttccgt atttacatcg ctttgatctc
     8041 attaaaggta aagaaaatca actttacttt ataggttgta caattgctaa cagtaaagcc
     8101 tacactgagg attggagtga taaaggccga atttttgttg ctcgttttaa tactcaaaac
     8161 catacattgg aaggattgca acaattgcct caaactttat taaaaaatca tggatactat
     8221 gccattcagg atgaaggata ttcattgatt acttcagtag aaggggtact caaactcact
     8281 tatccagaat tttctactac aggcgactgg caattagaac ggcttttcga tgaggagaca
     8341 agcgatgtgg tgaaagtgga tattaatcag gatggtaagg atgagtatgt gatcatccaa
     8401 ggttttcatg gagatcgttt acgtatcttc actgaagatt tcggtcgaga attattccat
     8461 tatcctgaaa aaaccccatt tggtcacgct atttggagtg gtcgtttact taatcagact
     8521 tgtttcgtat tcgggtggcg atcagaaaaa gcagaattaa ggctttttca ctttgtagat
     8581 gggcacttgg tttcagaatt agtagatgca aaagcagctt ctagtaatgt cttagctttt
     8641 gaaaaagatg gaaaagctta tcttttctca gccaataacg gacgtggcga agttgctctt
     8701 tatcaattag taaaataaat ttaagaagga gacaatatgg acaagaaaaa aatcttagta
     8761 acgggtattg tgcctaaaga aggtctaaga aagcttatgg accgatttga tgttacttat
     8821 tcagaagatc gcccattttc acgtgactat gtgttagagc atttatctga atatgacgga
     8881 tggttactca tgggacaaaa aggtgataaa gagatgattg atgcaggtga aaacttacaa
     8941 attatttctt taaatgcggt tggttttgat catgtagata ctgcgtatgc taaagaaaaa
     9001 ggaattgtag tctctaattc ccctcaagca gtacgagttc cgacagcgga aatgactttt
     9061 gctttaattt tagctgccag taagcgcctt gcattttatg atagtattgt aagaagtgga
     9121 gagtggattg atccgagcga acaacgttat caaggtttga ctttgcaagg ttcaacatta
     9181 ggtatttacg gaatgggacg catcggtttg acagtcgcaa attttgctaa agcatttggg
     9241 atgactgttg tttacaatga tgtttatcga cttcctgaag ataaggaaaa agagcttggt
     9301 gtaacctatc tcgaatttga tcagcttatt gaaacagctg atgtcataac catccacgcc
     9361 ccagcattac catcaacaat ccacaaattc aataaagatg tttttgctaa gatgaaaaat
     9421 cgaagctact taatcaacgc tgctagaggc ccaatcgttt ctgaagaagc acttattgaa
     9481 gctctgaaag aaggtgaaat cgcgggtgcg ggattagatg tttttgagaa tgaaccacag
     9541 gtatctgaag gcttgagaag tcttgataat gttatcatga gtccacatgc tgggacaggt
     9601 actattgagg gacgtagaac tttggctgaa gaagcagctg acaacatcat tgccttcttt
     9661 gatggcaagc cacaaaatat tgtcaataag taattggcta gtgagtgcag agccatcttt
     9721 tgaaaaaatg ggacctccca tttttttacg ggtaaatgta agcgcttaat aatttaataa
     9781 aggtgaactt atgctagaag ctttacataa attctttgaa gtttttggtg cagcaactgt
     9841 tgtacccgct atgattttta ttgtcagcct ttttctaaag gtcaatccaa ggcgagcttt
     9901 ctttagcgcc ttacgagctg gtgttggact cactggcttt ggttggatta tatcagcttt
     9961 tgctcctatg gtgactaagt atatccagca gatggttaaa actactggtc tccatttacc
    10021 aattgtagat attggttggc aggctggttc tctcaccgcc ttttcttctg agattggcct
    10081 ctctttcttt gtttttggct tgctaattga attggggctt ttcttgcttg ggattacccg
    10141 agtatttgtc ccatctaatc tttggaataa ctttggctat atgatttggg gtacaatggc
    10201 ctatgcagca acaggtaatt ttatattgtc tttcgcattt atggtttttg tcttactcta
    10261 ttcattagtg atgtcagaag tattggcaga tcgatggtcg gagtattacg gtgttaaaaa
    10321 tgcaacgatc aactcgattc ataatattga aacccttatt ccagctctta ttcttgatcc
    10381 tctttggaac ctacttggtg tcaataaggt aaagttgaat ccagaatcat taaagactaa
    10441 gttagggata tttggtgagc caatgacact tggttttata cttggtgtga ttattggtgt
    10501 attaggtagt ttacgtaacc ttgcaagtat tgatacttgg ggaggtatac ttggttttgc
    10561 agttgctctt gcagcggtta tgactatttt tcctttgatt acgggggtat ttgctagtgc
    10621 ttttgctcct cttgctgaag cagttgaacg caataagaaa aaagaaagtc aagctgagca
    10681 aggagcactg gataaaaaac gttggtttat tgcagtagat gacggtgttg gttttggaga
    10741 accagcaact attatcgcag gtctcatttt agtaccaata atggttgtta tttccttgat
    10801 tttacctgga aatgaagctt taccagtcgt tgaccttatt gctattcctt ttatgattga
    10861 agctatgata gctgtgtcaa aaggaaatat ccttaaagct attttgaatg gaatcatatg
    10921 gttttcacta ggtctttatg ctgctagtgc cctaggtcca atctacacag aagctgttaa
    10981 gcattttgga actgctttac cagcaggggt aactttgatt atgagcttta atctcctagc
    11041 tcatcctttg acagctctag tcttctttgc ttggatatct gggaatccac tctatattgg
    11101 tgtgacagtt attatctatc ttgctttctt gttcttgctt cgtactcgtc gtgaatctat
    11161 ttacgcatac cttaaaacta tggctgataa gaatagcgga tatgaaggta gttgtgaaaa
    11221 aatctttatt gctgaagatt aaaaatctgt tggaggaata ctcatgaaaa aaattgatgg
    11281 acatgctcat attgttgata caattgcagg atttaatggt aaagggcgtc ttaatgccct
    11341 tggaaacggt tatgctattt gggatgatgg tgatttaatt aagctcatcc cagacgaata
    11401 cggtgacaaa gcatttacag ctgaatcttt tttgaagtat atggatgcta atggtgtaga
    11461 taaagcagtc attttgcaag gtcatcttaa tggttatcaa aattattata cgcatttagc
    11521 tattaaacgt tatccagaac gttttacagg cgcattctct gttgatccat ttgcagataa
    11581 tgcaatgcaa attgtaaaac gtcatgttga agtccttggt tttcgagcta ttaaatttga
    11641 aattagtcaa ggagggggta tccatggata ccgtggacaa aaaacgcctt tccgtcttga
    11701 tacagaccca catgtgagtc gtattttgac ttatctgtta gactatccag gatttgtagt
    11761 tacagttgat tatggcaatt gggatcaaat tagtcaccaa cctgatgcta ttgctaattt
    11821 agctcgcctt tatacgagtc tcgattttgt tgtttgtcac ctctcttttc cacatgttga
    11881 gcattcaaat cgattgcgtg ctgaacttaa tatgtggaaa aacttcaaca atatctatac
    11941 tgatatttca gctattcaag atattgatgc cccagatagt tttccttttc caaaatcaga
    12001 acaaaatgta cgtattgcta aagaagttct tggggctaag cgtatcattt ggggaactga
    12061 ttctccatgg tcagcgacct tcaatactta cgaagaatta gctacttggc tagagaatgt
    12121 tgatattttc agtcaagagg aactagaaga tgttatgtac aataatgctg agcgtgttta
    12181 ctttaaagaa gaacatgtaa aggctaacag agcaggtata gatgatacaa ccaaagagct
    12241 caatctttat tagaagagtt ttcaaggtac aagagtttct tgtacctcct ttttaaatca
    12301 acaatgagga gacaaattat gacttactac cttagcattg actacggcgg tacaaatacc
    12361 aaggcgctta tttttgacaa attaggacac caaatcgctg tttcgagttt tgaaacttta
    12421 aaaaatgagg ctcaatctgg tcatcgtcaa gtaaaccttg ttaaaacgtg gaatgctata
    12481 acttctgcta ttagagaggt tattcaaatc tcaaaactca gccctgagca gattagtgca
    12541 gtagcatgta ttggacatgg gaaaggtctt tatctgctag ataataagtt ggagccactt
    12601 gaacaaggaa ttttgtctac agataatcgt gccaaagatt tggcgcaata tttcgaatct
    12661 aaacttgata atatttggga gttgactcgg caacatattt tcccttcaca aagtccagtt
    12721 attttacgtt ggcttaaaga ttatcagccc gaaacctata aatcaatagg ggcggtcctt
    12781 tctgcaaagg actttattcg gtataagctt acaggaaaag tacagcaaga atatggtgac
    12841 gcttcaggta atcattggat aaattttcaa acaggaactt atgatccagc tattttagat
    12901 ttttttggca ttagagagat agaaaactca cttcctgaac ttatagatag tgcagattta
    12961 gttcctgggg gaattagttc tcaagcagca aaagagactg gtcttgtaga aggaaccccc
    13021 gttgttggag ggctctttga catcgatgct tgtgctcttg gatcaggtgt tttagagtca
    13081 gatactttta gtgttatttc gggaacttgg aatattaata cttatccaag tttaaaacca
    13141 gcaaagcaag atagtggtct tatgacttcc tattttccag atcgtcgtta tctcttagag
    13201 gcaagtagcc ctacttctgc agggaatctt gattttatgt taaaaatgct catgcatcaa
    13261 gaaattgata acgctaaatc tagtggaggt tctatctatg ataatttaga agaatttctc
    13321 actcatactg atgccacaca tcatggactt attttctttc cgtttcttta cggtagtaac
    13381 acatcacaag atgctagcgc ttgctttttt gggctaacaa ctaaatcgac gaaatctcag
    13441 atgatacgtg cggtatatga aggtattgcg tttgcacata agcagcatat cactgattta
    13501 ataaaaagta ggggcggtgt gccaaaaata attcgtttct ctggcggagc taccaactca
    13561 ccagcatgga tgcaaatgtt ttctgatatc ttaaaccttc ctattgaaac agtagaaggc
    13621 acagaattag gaggattagg aggagctatt ttagcacgtc atgctttaga taagatttcg
    13681 ttaaaggaag cagtccaaga tatggttcgt gtaaaagcta tttataaacc tcaattatcc
    13741 gaagtaaagg ggtacgaaaa aaaatatcac gcctaccaaa aattattgga aacactggat
    13801 cctatttggt cggaactcgg tcatctgaat aagtagggat atcaaaggag ttgttatgaa
    13861 acaagaagat attattatca atagtattgt attcaaaaaa cagttagaca aaggttttac
    13921 acaagttcag ctcattaagt tagtaaagga tttaggattt tcacgatttg agatacgtca
    13981 agaattgctt caagatccag atagagagct acccgcttta aaagcagagg ctgactttta
    14041 tgatatcaat ctttactata gtgctaatga agaccttatc aaaggaggga aagttaatcc
    14101 ttatcttaat aaaggcctta aagaagcatc acagttagga gctcctttca ttaagctaaa
    14161 tgttggacaa accagaaact taagtaaaga agagttagaa cctcttaaag agatattgaa
    14221 aagccaaact attggtataa aggtggaaaa taatcaagac cctaaagcag ctacagtgga
    14281 gaattgccaa tattttatga ctctagtgaa agaattacaa ataccaataa gttttgtttt
    14341 tgatactgct aattgggcct ttatcaacca agacctatac caagcggtaa ataacttagc
    14401 ttgtgatacc acctaccttc actgtaaaaa ttttatacag gtagctggaa agccccacct
    14461 ctcgaagagc ctcttcgaag gagagattaa tctgactgat ttgttaaaga gtttctctaa
    14521 ttgtgagtat ctagctctag aataccccac agagttggag atattaaaaa gggatgttca
    14581 acgtttaatc tctatcagta atagtcaaaa cttttaatga aaaggcttta aaaagatgtt
    14641 ataataagtc tgtatgaatt cgagtagaaa agaggtatat tcttgattat tctcgataaa
    14701 aaaagttatg acctcctctt ttacctattg aaattagagg aacctgaaac agttatggca
    14761 attgccaacg cacttaatca gtctagacgt aaagtgtatt atcacttaga gaagataaac
    14821 gatgcactgc ctagcgatgt gcctcagatt gttagttatc cacgtgtagg aatcttgcta
    14881 acagaaaaac aaaaagcagc ctgtcgtctt ttattagatg aagtgactga ttatagttac
    14941 gtcatgaaaa gtagtgagag gttgcagctg tctttagtat ctatcgtagt agctaaggac
    15001 cgcataacga ttgataggtt gatgcaacta aacgatgttt ctcgaaatac tatcttaaac
    15061 gatttaaacg aattaagaag tgagttagca gagaaagaat ataatttaca gttacaatca
    15121 acaaaatgtc gtggttattt tttagatggt catccattgt ccattatcca gtacttatat
    15181 aagctcttag atgatatcta ccataatgga agtagtagtt ttatagacct ttttaatcat
    15241 aaactgtctc aagcttttgg tgccagcact tatttttcta aagaggttct tgattatttt
    15301 catcattatc tcttcatttc tcaacgaagt ctaggtaaga aaatcaacag tcaagatggt
    15361 cagtttatga ttcagatttt gccttttata ctaatggctt atcgtaagat gcgattaagt
    15421 cctgaagtac agacctctct taatagtgat tttagcttgg tttggcaacg taaggaatat
    15481 gagattgcta aagagttggc tgatgagctg gaagaaaatt ttcagttatc actggatgag
    15541 attgaagtgg gactagtagc catgcttatg cttagtttcc gcaaggaccg tgacaatcat
    15601 ttagagagcc aggattatga tgatatgcga gctactctaa ccagtttttt gaaagaattg
    15661 gaagaacgat atcaccttca ctttgttcat aaaaaggact tactaagaca acttcttact
    15721 cactgcaagg cactcttata tcgtaaacgt tatggtattt tttctgttaa tcctttaaca
    15781 gagcatatta aagacaaata tgaagaactt tttgccataa cctcgtcttc tgtaaagctt
    15841 ttagagaaag cttggcaaat caaattgacc gatgatgatg tagcatatct aacgattcat
    15901 ttaggagggg aacttcgtaa tagtcaacaa tctcctaata aacttaagtt agttattgta
    15961 tctgatgaag gaatagcgat tcagaaactt cttttaaagc aatgtcaacg ctacttaaca
    16021 aatagtgata tagaagctgt ttttacaacc gaacagtatc aaagtgtgag tgatcttatg
    16081 catgtagata tggttgtctc tactagtgac gctttagaat ctcgttttcc gatgttagta
    16141 gttcaccctg ttttgacaga tgatgatatt attcgcttga ttcgcttttc taaaaaaggt
    16201 aactgtgcaa atagtaatca atttaccaat gaacttgaaa aaacaattgc tcaatatgtc
    16261 aaggaagata gtgaacgcta cgtgctgaaa tctaagattg agaaacttat tcatcaagaa
    16321 ttgctccaag acgtccttcc ccttcaaagt acagtttgtt aaaatataat tttatggtag
    16381 cctctcagct tttctgaaga cagaaaaaga agctggggct tttttggtgc ttttaaacaa
    16441 tgtgctagat atttaatcgc aatgagttcc tctatgacat gataattaaa gttgagaaag
    16501 ctagtacttt taatgggagt actttttatt tgtgcttatt ttaattaaaa ttatgagtct
    16561 aatttggaac agacagccca gttttgaaca cataaatgtg tgacacttgg tctttttttc
    16621 aaaagtattt gaacttatac tagtagatgt aagaaacaat taatcaggag gaaataaaat
    16681 gcctaacgtt aaagatatta ctagagaatc atggatttta tcaacattcc cagaatgggg
    16741 tacatggcta aacgaagaaa ttgaagaaga agttgttgct gagggcaact ttgcaatgtg
    16801 gtggttagga aactgtggtg tttggattaa aacaccaggt ggtgctaatg ttgttatgga
    16861 cctatggtca aaccgcggta aatcaacaaa aaaagtaaaa gatatggttc gtggacacca
    16921 aatggctaat atggcaggtg ttcgtaaact ccaaccaaac ttacgcgcac aaccaatggt
    16981 tattgatcca tttgctatta atgaattgga ttactatctt gtttcacatt tccacagtga
    17041 ccacattgat atcaacactg cggcagccat tattaataat cctaacttag accatgttaa
    17101 atttgttggg ccatatgaat gtggtgaaat ttggaaaaaa tggggagttc cagaagaacg
    17161 aatcatcgtg attaaaccag gtgaatcatt tgagtttaaa gatatcaaag ttactgctgt
    17221 tgaatcattt gaccgtactt gcttggtgac acttccagta gatggtgcag aagaacatga
    17281 tggagaactt gctggtttag ccgtgactga tgaagaaatg gctcgtaaag cagttaacta
    17341 tatttttgaa acaccaggtg gaacaatcta tcatggagct gactcacatt tttcaaatta
    17401 ttttgctaaa catggtaaag actacaagat tgacgtagct attaacaact atggtgataa
    17461 cccagttggt attcaggata aaatgacttc aatcgatctt cttcgtatgg cagaaaatct
    17521 ccgtgccaaa gtcattattc ctgtacacta cgatatctgg tctaacttca tggcttctac
    17581 agacgaaatc ttacaattgt ggaaaatgcg taaagaacgc cttcaatatg acttccatcc
    17641 atttatttgg gaagttggag gtaaatacac ttatccacaa gataaagata gaattgaata
    17701 ccatcaccca cgtggttttg atgattgctt cgaacaagaa tcaaatattc aatttaaagc
    17761 acttctataa tggctaatga gagactgtat gtcgcatgat atgcagtttt tcaattttaa
    17821 aatatgttac taaaatcaca attaagatta ggagaaataa tgtcagaatt tgatacaaaa
    17881 tcatatttag aaaaacttga tgcatggtgg agagcagcta attatatttc tgcagcacaa
    17941 atgtatctaa aggataaccc tctcttgaga cgagagctgg ttgaaaacga cttaaaggtt
    18001 catccaattg gtcactgggg cactgtacct ggacaaaatt ttatctatgc tcacttaaat
    18061 cgcgctatta ataaatacga tttagacatg ttctatattg aaggtccagg tcatggtggt
    18121 caagttatgg tatctaattc atatttagat ggttcatata ctgaattaaa cccaaatatt
    18181 gagcaaacag aggatggctt taagcagtta tgtaaaatct tctctttccc aggtggaatt
    18241 gcatcccatg ctgcaccaga aacaccaggg tcaattcatg aaggcggtga acttggttac
    18301 gcactttctc atgcgacagg tgccatccta gacaatccag atgttattgc tgcgactgtt
    18361 attggtgatg gtgaaggtga aacaggacca cttatggctg gttggctatc taataccttt
    18421 atcaatccag taaatgatgg tgctgttcta ccaatcttct atttaaatgg tggtaaaatc
    18481 cataacccaa ctatctttga acgtaagaca gatgaagaat tatctcagtt ttttgaagga
    18541 ttaggttgga aacctatttt tgcagatgtt gttgaactct ctgaggatca tgcggctgct
    18601 catgctttgt ttgcagaaaa attagatcaa gctattcaag agattaaaac cattcaatca
    18661 gaagcacgac aaaaaccagc agaagaagct atccaagcaa aattccctgt cttagttgca
    18721 cgtattccta agggttggac tggtccaaaa gcttgggaag gaacaccaat tgaaggtggc
    18781 ttccgcgctc accaagtacc aattccagta gatgcccacc atatggaaca tgttgattct
    18841 cttttgtcat ggcttcaatc ataccgtcca gaagaattat ttgatgaaaa tggtaaaatc
    18901 gttgatgaga ttgctgctat ttcaccaaaa ggtgatcgtc gcatgtccat gaatccaata
    18961 accaatgctg gtattgttaa agcaatggat acagcagatt ggaagaaatt tgctcttgat
    19021 attaatgttc caggtcaaat tatggcacaa gatatgattg aatttggaaa atatgcagca
    19081 gatttggtag atgctaatcc agataatttc cgtatttttg gtccagatga aacgaaatca
    19141 aatcgtcttc aagaagtgtt tacacgtact agccgtcaat ggcttggtcg tcgtaaacca
    19201 gattatgacg aagctttaag tccagctgga cgtgttattg attcccaatt gtctgaacac
    19261 caagcagaag gtttcttaga aggttatgtt ttaactggtc gtcacggctt ctttgcttca
    19321 tacgaatcat tccttcgcgt tgtagattca atggtaacac aacatttcaa atggttacgt
    19381 aaatcaaaaa cacacacaac atggcgtaaa aattatccag cgcttaacct aattgcagct
    19441 tcaactgttt tccaacaaga tcataatggt tatactcacc aagatccagg tattttaact
    19501 catttagctg aaaaaacacc tgaatacatt cgtgaatatt taccagcaga tactaactca
    19561 cttctagctg ttatggataa agcgtttaaa gctgaagata aaattaattt aattgtgaca
    19621 tctaagcacc ctcgtccaca attttactct attgctgaag cagaagagtt agtggcagaa
    19681 ggatataagg tgattgactg ggcttcaaat gtgtcgctta atcaagagcc agatgttgtc
    19741 tttgctgcgg cgggaacaga acctaactta gaagctttgg cagctattag cattcttcat
    19801 aaggctttcc cagaacttaa gattagattt gttaatgtat tagacatcct aaaacttcgt
    19861 catccttcac aagatgctcg cggtttatca gatgaagagt ttaacaaagt ctttaccaca
    19921 gataaacctg ttatttttgc tttccatggt tatgaagata tgattcgaga tattttcttt
    19981 agtcgtcaca atcataattt gcatacgcat ggttaccgtg aaaatggtga catcacaaca
    20041 ccatttgata tgcgcgtgat gtcagaattg gaccgattcc atctggcaca ggatgcagct
    20101 cttgcttctt taggaaataa agcacaagcc ttcagtgatg aaatgaatca aatggttgct
    20161 tatcacaaag actatattcg tgaacacgga gacgatattc cagaagtaca aaattggaaa
    20221 tgggaaaata tcaagtagtc acttttttca ggcctatact tacaaacaaa aacactttta
    20281 gattttttct aagggtgttt tgttatttta tagaagaatg accgcttttt caaaagtatt
    20341 aagtggtcac ttttaaagtt gtaatattaa tactaataag tctttaaata attcaataat
    20401 gccataacaa taatggtgtg ggctaattaa aatatctcta agcaaaggtg ttgacataaa
    20461 gagaccatac atgatattat agttatgttc tgtttcggca gtttattttt ttgagtaaaa
    20521 aagtgactac ccttttacga taacttaacg tcactttata gataagacac ataaccttaa
    20581 aaataagata cgaaattttt agtaggagat aagaatgaca aatattttag aagtgaagaa
    20641 tttaaccaaa atttttggga aaaagcaaaa agctgctctt gaaatggtta agcaaggaaa
    20701 aagtaagaca gaaattcttg aaaaaacagg agcgactgta ggtgtctatg atgcgagttt
    20761 tgaaataaaa gaaggcgaaa ttttcgttat catgggactt tctggttcag gaaaatcaac
    20821 tttagttcgg atgttaaacc gtttgattga cccatcatca ggaaatatct atttagatgg
    20881 taaagatatt gcaaagatga atgtagaaga tttacgtaac attcgtcgtc atgatatcaa
    20941 catggttttc caaaattttg gtcttttccc acatcgtaca attttagaaa atacagagtt
    21001 tgggttagag atgcgtggtg tttctaagga agaacgtaca acgttagctg aaaaagcttt
    21061 ggacaatgca ggtctattac cgtttaaaga tcagtacccc agtcaattat caggaggaat
    21121 gcaacagcgt gttggtttag cacgtgccct tgctaacagc cctaaaatcc ttctgatgga
    21181 tgaggccttc tcagctttag acccattaat tcgtcgtgaa atgcaagatg agcttcttga
    21241 tcttcaagac acgaataaac aaactattat ttttatcagc catgatttga atgaagcgtt
    21301 acgtattggt gatcgtattg ctctgatgaa agatggagaa atcatgcaga ttggtacagg
    21361 agaagagatt ttgactaatc cggctaatga ttttgttcgt gagtttgttg aagacgttga
    21421 tcgctcaaaa gtgttaactg ctcaaaatat tatgataaaa cctttaacaa ctgttttaga
    21481 aattgatggc ccacaagttg ctttaacaag aatgcatcgt gaggaagtct caatgttaat
    21541 ggcaacgaat cgtcgtcgcc aacttctggg atccctaact gcagatgctg caattgaagc
    21601 tcgtaagaaa ggtttgccgc tttctgaagt gattgataaa gatgttgtaa cggtctcaaa
    21661 agatacagtt attacagata tcatgccttt aatttatgat tcatctgctc cgatagccgt
    21721 tactgatgat aatgatagac tcctaggggt tatcattaga ggacgtgtga tcgaagcact
    21781 tgctaacgtt caagatgaaa cagtagtaga atcgccaaaa gaaacggtag aagcttaata
    21841 gagaggacaa ttcattttgg aaaatttatt acaacataag ttaccggtcg caccatttgt
    21901 agagtcaacg accaattgga ttacgaaaac tttttctggt ttatttgact ttatccagac
    21961 aatcggtaat gcattgatgg attggatgac aaaaacatta ctattcatta atcctttact
    22021 ttttattgta ctaattacaa ttgctgtatt tttcttagct aagaaaaaat ggcaattacc
    22081 gacatttact ttcattggtt tgctatttat ctataaccaa gggctgtggg aacagttgat
    22141 taatactttt aacttggttt tagtagcaag tttaatttct attattattg gtgtaccact
    22201 aggtatttgg atggcaaaaa gtgataaagt taaacaagtt gttaatccaa ttcttgactt
    22261 tatgcaaact atgccggcat tcgtttacct tattcctgca gtagccttct ttggtatcgg
    22321 tatggtacca ggtgtttttg cctctgttgt atttgctctg cctccaacag tgcgctttac
    22381 taacttggct attcgtgaaa tcccacttga attaatagaa gcatctgatt catttggttc
    22441 aacagctaag caaaaattat tcaaagttga actaccactt gctaagaaca ctattatggc
    22501 aggaatcaat caaactatga tgttggctct ttctatggtg gttacaggat ctatgatcgg
    22561 tgcacctgga cttggtcgtg aggtgttatc ggcgcttcaa catgctgata ttggtacagg
    22621 atttgttagt ggtctatcgc tagttatttt agcaatcgtg ttagaccgtg ttagtcaatt
    22681 ctttaattcc aaaccgggtg agaagcaagc taaaacatca aaagttaaaa aatgggtagg
    22741 tttaggggcc ctggctctct tcattctagc agctcttggt cgcattgttg tcaatatgac
    22801 ttcaggaaat gaagccaaag gtcaaaaagt gaagatagct tatgtccaat gggattcaga
    22861 agttgcttca accaacgtta ttgctgaagt tttaaaatct aaaggatatg atgttgaatt
    22921 gacaccactt gacaatgcgg tcatgtggca aacagtcgcc aatgggaatg ctgattttac
    22981 aacaagtgct tggttaccaa agacccacgg gcaatatttt aataaataca aaaatagttt
    23041 agatgactta ggtcctcacg ttgagaacgt caaaataggt ttagttgttc ctaaatacat
    23101 gaacgtaaat agtattgaag aattatcaaa tcaagcagat aaacaaatta caggtatcga
    23161 accaggtgca ggtattatga agtctgctaa acaatccctt aaagattatc caaacctttc
    23221 aagttggaag ttgttatcag catcaacagg agctatgaca acaaccttag ggaaggctat
    23281 caaaaacaaa gatcaagtag tcattacagg ttggtcacct cactggatgt ttgctaagta
    23341 tgatttaaaa taccttaaag atcctaagaa atcatttggt ggtgaagaac atatcaatac
    23401 aatagcacgc aagaatttga aaaaagatat gccaaaagta tataaaatta tcgataagtt
    23461 caagtggact aaagaagata tggaatctat catgttagat atggataaag gtatggagcc
    23521 tgctaaagca gctcaaaaat ggattaaaaa ccacaaaaaa gaagtttcag agtggactaa
    23581 atagtataga aaaaaacacg tctctcttag agcgtgtttt ttaatgtgac ttaagtcgtt
    23641 ttagctattt aatataagtt ttatattatt ttaatacatc aaaagtgaga gaatgaaaga
    23701 aatggcaggt tataaagtat tac
//
LOCUS       DATRNX010000020        23632 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRNX010000020 DATRNX010000000
VERSION     DATRNX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23632
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            907..2325
                     /locus_tag="VBC81_000641"
     CDS             907..2325
                     /locus_tag="VBC81_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO6911700.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            2596..3555
                     /locus_tag="VBC81_000642"
     CDS             2596..3555
                     /locus_tag="VBC81_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6911701.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            3716..4618
                     /locus_tag="VBC81_000643"
     CDS             3716..4618
                     /locus_tag="VBC81_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6911702.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            4778..5842
                     /locus_tag="VBC81_000644"
     CDS             4778..5842
                     /locus_tag="VBC81_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6911703.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            5957..6949
                     /locus_tag="VBC81_000645"
     CDS             5957..6949
                     /locus_tag="VBC81_000645"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6911704.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(6994..7443)
                     /locus_tag="VBC81_000646"
     CDS             complement(6994..7443)
                     /locus_tag="VBC81_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6911705.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            7409..8113
                     /gene="rsmD"
                     /locus_tag="VBC81_000647"
     CDS             7409..8113
                     /gene="rsmD"
                     /locus_tag="VBC81_000647"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6911706.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            8125..8415
                     /locus_tag="VBC81_000648"
     CDS             8125..8415
                     /locus_tag="VBC81_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911707.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            8412..8897
                     /gene="coaD"
                     /locus_tag="VBC81_000649"
     CDS             8412..8897
                     /gene="coaD"
                     /locus_tag="VBC81_000649"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6911708.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            8887..9960
                     /locus_tag="VBC81_000650"
     CDS             8887..9960
                     /locus_tag="VBC81_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6911709.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            10035..11369
                     /locus_tag="VBC81_000651"
     CDS             10035..11369
                     /locus_tag="VBC81_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6911710.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            11438..12016
                     /locus_tag="VBC81_000652"
     CDS             11438..12016
                     /locus_tag="VBC81_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6911711.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            12067..13173
                     /gene="rlmN"
                     /locus_tag="VBC81_000653"
     CDS             12067..13173
                     /gene="rlmN"
                     /locus_tag="VBC81_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6911712.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            13173..13688
                     /locus_tag="VBC81_000654"
     CDS             13173..13688
                     /locus_tag="VBC81_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6911713.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            13884..15629
                     /locus_tag="VBC81_000655"
     CDS             13884..15629
                     /locus_tag="VBC81_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6911714.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            15619..17358
                     /locus_tag="VBC81_000656"
     CDS             15619..17358
                     /locus_tag="VBC81_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911715.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            17486..18052
                     /locus_tag="VBC81_000657"
     CDS             17486..18052
                     /locus_tag="VBC81_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6911716.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(18120..18658)
                     /locus_tag="VBC81_000658"
                     /pseudo
     CDS             complement(18120..18658)
                     /locus_tag="VBC81_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(18659..19651)
                     /gene="bioB"
                     /locus_tag="VBC81_000659"
     CDS             complement(18659..19651)
                     /gene="bioB"
                     /locus_tag="VBC81_000659"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6911717.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            19776..20291
                     /locus_tag="VBC81_000660"
     CDS             19776..20291
                     /locus_tag="VBC81_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911718.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20281..21396
                     /locus_tag="VBC81_000661"
     CDS             20281..21396
                     /locus_tag="VBC81_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6911719.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            21389..22618
                     /locus_tag="VBC81_000662"
     CDS             21389..22618
                     /locus_tag="VBC81_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6911720.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            22731..23363
                     /gene="nth"
                     /locus_tag="VBC81_000663"
     CDS             22731..23363
                     /gene="nth"
                     /locus_tag="VBC81_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6911721.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23364..>23632)
                     /locus_tag="VBC81_000664"
     CDS             complement(23364..>23632)
                     /locus_tag="VBC81_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6911722.1"
                     /translation="PITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQML
                     FIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         7680 a         3635 c         4706 g         7611 t
ORIGIN      
        1 aatgattcat aatattagta gtattttcta ttttctatta tctatcaatt tgggtttgtt
       61 tagtagtttt aacattcctt aaaaacttaa ttttataacg agcaaacaaa aatatatgct
      121 aatctacttc tttttatgcg taaagatata ttttaaaatg acaatcagat tataaaggaa
      181 cataatgcta aaaatgaata gacatagaaa gtgaagatac atgaggactt tataaatagc
      241 aggaaagtag atactagtta gatatagtag tgcaagatag atgagaaaaa ataacgaata
      301 ctttaacttg gtcataacaa ttccattcta atggtagtaa gcatgcataa aatgtaggtt
      361 ttatcatact acttgaatcc agcaactaga tagagggggt tacatacgat aagacagtag
      421 tattttaaac cgatcggttg acttattgat actgtttagg gaacaagtgt tttgttgtca
      481 atgttaaatt taatttagca ttttgatggt atcaccgcaa gaatgaaagg aaaagttgta
      541 tgtgctgaaa aaactaatgc ttggcgattt agaacacacg tttactagtc gagatgacaa
      601 aaaataagta ccaaatgtaa aggtcgatat tatcagcatt gtcagcgtta ggtgttataa
      661 aaatttaaca tcttccaatt aagctaggtt taagtttcct aagttgaact acgcctagcc
      721 gaaaaataat ttctttttta cagaactcct aacaaggatt ttgaattgtc aagattcttg
      781 tttttttgga aagtcattta gaggcttttt gggggaagta ttaactattg tatccgttta
      841 caattccttt caaaaatgtt agtatatcat tgagggatat gagttatgga taagggagag
      901 aggatgatga tgactaagga aacgttggca cattatcaaa agaaaataga acaagaatct
      961 gaaaaaaagc agtctttaga tgagcactca tggcatgtgg cctgttttag tcgacaggaa
     1021 gcatctataa ttggtcaagg agatgtgctt tttttaattg gtctatacca cgatctaggc
     1081 aaagctgatc gagcctttca agataaatta ttaaataatc caaatcgaca tgttgatcat
     1141 tcttatgcag gggcaaaata cttatgctct attattgggc ctcatctaaa aagtcgaggg
     1201 gttgataaag gtgagagaat gacattcaat gaaatggtgg gttatgtcat ctcggctcat
     1261 catgggatgt atgatttttg ctactgttct gacgatgctg aatattatag ctttaataaa
     1321 tttaaaaacc gtatcaacag agacttagat gattatcact atcacgaaga tattaaaggg
     1381 tatgctataa aattagaaga aaaattgtgt gattacggct acaaagattt aagggagctt
     1441 attgataaag cttttgataa ttaccaacaa gctatgtctt ccttaaactg gcaagataat
     1501 agtgagtggg attattatca gtcttgtatg gtgagacttt acttgtcgct cttaaaaaac
     1561 gctgatattt tggacacagt aaatgcctat ggtcttaaaa taagtcctat ggataaaaca
     1621 gagcgatcct ctctaaaaca ttcttattta gcagccattg aacaaaaata tgctagcttt
     1681 ggacgaccaa acaatcagtt gaatactatt cggacagaaa ttgctgagcg tgttaaagaa
     1741 agaggtaaac gggattccaa agggatctat cgcttagatt taccgacagg agctggcaag
     1801 actaatctta gtatgcgcta tgcgtttcac caattagttc atcaagacaa atcaagattt
     1861 ttttacataa cgccctttct ttcggttctt gagcaaaatg cttccgaaat tagaaaagtt
     1921 acgggtgacc ttggcgttct agaacaccat tccaatatgg ttaaacaggc taatgaagat
     1981 gatgacaagg acagtttatt gtcagcttat cttattgata gctgggatag tcaagtagtc
     2041 ttgacttcta tggttcaatt tttccaaacg ctttttaaaa caaaatcggc taatttgaga
     2101 cgtttttcaa gtttgattaa tagtgttgtg attctagatg aagttcagtc actgcctatt
     2161 gaagtcacca ctttgtttaa tttaacgatg aattttttta ataaagttat ggatacaacc
     2221 atcgttcttt gtacagcaac acaacctgct tatgattctt cagagattga ccatcgtatc
     2281 tgttatggag ggaatttggg agaattagct gaaatagtgg attgaaataa gtttatacgt
     2341 tgcttcatca ccaagtttta cgtcgcaccc attgcgggtg cgtggattga aatataagtt
     2401 cagcaacttc acgagaaaat gttcctcgtc gcaccctttg cgggtgtgta gtttcaacta
     2461 ttttaaatct ttcaaattat gctattaaaa ttgtctaact tttaacagag atgcgattga
     2521 tttgtattaa tccctaaaaa gataaattgc tagttcttat gctataataa ctctatcaat
     2581 gtcaaaggag tttttatgtt aaaggtaaga tatggtgttg tgtcaacggc aaaggtggcg
     2641 cctcgtttta ttgaaggagt ccgcttagca ggaaatggtg aagttgtggc tgtatcgagt
     2701 cgaactcttg agtctgcgca agcttttgcc aacaaatatc atctcccaaa ggcttacgat
     2761 aagttagagg atatgttagc agatgagtcg attgatgtga tttatgtggc aacaattaat
     2821 caagaccatt ataaagttgc taaagcagct ctgttagcgg gaaaacatgt gttggtagaa
     2881 aagcctttca cattgactta cgaccaagca aatgaattat tcactttggc agaaagttgt
     2941 aatctgtttt tgatggaagc acaaaaatcg gtatttattc cgatgacaca agtgattaaa
     3001 aaattacttg cctctggtga aataggagaa gtgatctcca tctcatctac aacagcctac
     3061 ccaaacattg accatgtaac atggtttaga gaacttgagt taggtggagg aacagtgcat
     3121 tttatggcgc cctatgccct gtcttattta caatacctat ttgatgcgac tataactcat
     3181 gctagtggga ctgcgacttt tccaaaaggt caaagcgata gtcaatccaa actactcttg
     3241 caattatcaa atggggtcct cgtagatatc ttcttgacaa cacgtttaaa tttaccgcat
     3301 gaaatgatta tttatggaac tgaaggtcgt ctaatcattc cacatttttg gaaaaccact
     3361 catgcaaaat tggtgagaaa tgatactagt gcgcgaacga ttcaagttga catggttagt
     3421 gattttgaga aagaagctta tcatgttagt caaatgattt tagaagggca aagggtaagt
     3481 catatcatga cgcctcagtt gacgctgtca ggtgttaaga ttatagaagg cttataccgt
     3541 tcatggggga aatgatgctg gctatctagc atattaataa ggagtactag atgttatttt
     3601 agtagtcctt ttttgttttg ctctcataag gttaattaga tttaaaaggg ttttctactt
     3661 gttttttctc gatttttgtt tcataatgta gacaagttta gagtgaggtg cttctatgtt
     3721 ttcgagcgat tttggatttg agataattaa tgttgacgat ctatccttac aagaggagaa
     3781 atttttacct tcagaattgc tggcttatgc acgtgatgaa aacgagtctt ctttcgtacg
     3841 tgatattgag gggcatttga cacttgttta ccagcttttg gatacgcaag gtcacgttga
     3901 tgatgttcgt catgtgccga gagtgatacc agtgactcta tttttgaaag aagatggctt
     3961 gtttgtatta gctaatcata aaaatattat ccttgtaaaa aaagcactta atcgcgttga
     4021 aaaggtagat tcgccgaaac acctcttatt atcattagtg actgctttct caaagcaata
     4081 ttttgatgtt ttagatacta tttctgaaga aagagataaa ctaatcaatg atttgcgtaa
     4141 aagaccaaac aaatcgaact tggcaaggct ggcaaattta caaagtggaa cagtacactt
     4201 aatgatggga actaagcaaa attttgaaat gctgaccgat ttacaaaaca ttgagcaaga
     4261 taaagagaat actagaaatg aaaagatgca acttcaagat gctattattg aagcaagaca
     4321 attatctaac atgtgttcct taaattcaca ggttttccaa gaactttctt cttcttataa
     4381 taatgtcctc agcaataacc ttaacgacaa tgtaactacc ttaacaatta tttccattgg
     4441 tatttccatt attgctatgg ttaccagctt ttatggaatg aatgttaagt tgccttttga
     4501 tagtgttgat gctgtctggg tattgattat tttaattacg actatcatta ctattatgct
     4561 ctctattgtt atgtacattt atgtccataa agaggataat catcccaaac ataaatagag
     4621 aattaataag agggtgctat caattagttt agttttaagc atggcaacat gctttttttg
     4681 tacaaagaaa ctagaattca tctttgactt tgagcttgct ttctaactct taaaacgaca
     4741 taatagagga taatgtacag tgaatagaga ggttactatg tcaatagaat cagatttttt
     4801 ccggaaaaaa cgttttattt tttcatcgct tgaggagttt ggatttatca aatctgacca
     4861 agagtatatc tattgtcaaa catttatgga taatgacttt aaagctatta tcacgatttc
     4921 attggatgga aagatagctg gtaaggttat tgattcggca cttgaagaag aatatttacc
     4981 gcttcgagca gcaaattata atggttcgtt tgttggcgaa gtacgatcgg cttatatggc
     5041 tatattaggt gatatttcag attcttgttg taaggattta ttatttacca aagatcaaag
     5101 taatcgttta gcggagaaga tagctacaac ttttgaagat tcagttgatt atccatttgc
     5161 gaagcaccca caatatgctt cttatcgtgt atcaggtaaa tggtatgctc ttctttttcc
     5221 attgaagatg gggaagctag aaaatgttcc agctcaactg tcagaagaag aggttgaagt
     5281 tttgaatatt aaagtcaatc ctcaagatat ggaaatactc ttacaaaaag aaggtattta
     5341 tccttcttat catatgtcta aaaagacttg ggtatctatc gttttagaca atactttgtc
     5401 tgatatagaa atttttaaat tggtatcaga tagtcgtaag cttgtttctc ccaataaaaa
     5461 atcaaattct gagccagagt tttggattat tcctgctaat cctaaatttt acgatattga
     5521 taaggagttt gcagagcatc aattgattga ttggactcaa aaaggaaaga ttgcggtggg
     5581 agattatgtg tttatttata ttacagcacc tattcgtgct ttgcgctatg tctgtcaagt
     5641 gatagaacgt gaccttaaaa caagtactta cttaggagca agagatgtca aagcttatat
     5701 gcgactcaaa ctgataaggc aatttgaaga ttctacctat cctgtagcat ttttagcaga
     5761 acacggcgtt aagactgtga gaggaccacg gcgaatgacc aaggaattgc gacaagtcat
     5821 ggcaaaaaca attctagagt aatgactttc agaaaaaaac ttgtaaaaaa gtcagaataa
     5881 ttctacaatt cctcttccat tttgttactt gaaggagtat aatagctcta acattttata
     5941 atttaaggag gaacatatga agaaaagctt tattcatcag caacaggaga tttcttttgt
     6001 caaaaatact tttacacaat atttgattga taagttagaa atcgtcgaag tgcaaggacc
     6061 tattcttagt caggtcgggg atggtatgca agataattta tctggtattg aacatcctgt
     6121 atcggtgaaa gtccttaata tacctgaagc tgaatttgaa gtcgttcact cattggctaa
     6181 atggaaacgt catactttgg caagatttgg gtttaatgag ggagaaggtc ttttcgttca
     6241 tatgaaagct cttcgtccag atgaagattc tcttgatcca acacactcag tttacgtaga
     6301 tcagtgggac tgggagaagg tcattcctga tggtagacgt aatttggact acctaaaaga
     6361 aactgtggaa aaaatctaca aagctatccg cctaacagag cttgctgtgg aagctcgttt
     6421 tgatattgaa tccattttac caaagcgtat tacctttatt catacagaag aattggtaga
     6481 aaaataccca gacttaagcc ctaaagagcg tgaaaacgcc atcgctaagg agtatggcgc
     6541 agtctttttg attggtattg gtggagagtt agcagatggt aaaccacatg atggtcgtgc
     6601 accggactat gatgactgga caacaccatc agaaaatggt tttaaaggtc tcaatggtga
     6661 tattttagta tggaacgaac agcttggaac tgcctttgaa ctatcatcaa tgggaattcg
     6721 agttgatgaa gatgctctta aacgtcaagt tgttctcacc ggtgatgaag atcgtttaga
     6781 gtttgagtgg cataagacgc tattacgtgg tttcttccct ctgactattg gtgggggaat
     6841 tggtcaatca cgattagcta tgttcttgct tcgtaaaaaa catattggag aagtacagtc
     6901 aagtgtctgg ccaaaagaag tcaaagatac cttcgaaaat atattataaa aaatcagcca
     6961 agcagttttg tttggctgat tttaacaata agtttatttt tggcttaaga aagtttggat
     7021 ttgttgaggt gtagcttggc ttccttgaac aagatattta gtcatttttc ctcgttgata
     7081 atgtgctaaa tttggaacag tggtaatatg atgctttttg aaaaaactct tcaatcgttt
     7141 ccctttgtat ttttgactat caaggtagta aatttttgaa tgaagtgtgg cttgagattt
     7201 taataatttt ggtaagaaag ctctacaata ttggcaagtt ggacgtccta taaaaatagt
     7261 cgcttcagat tgttgacttt tgcttactaa ggccttatct gtgatggcga ttaagtcact
     7321 atttgttatc atgtgttggt attgttgttg agaaatagtc atggtgtcag cgctacttaa
     7381 gtttgatttt agcgttgtaa ggcataatat ggctattaaa gttaataata attttttaat
     7441 cattatggga acctcgtttc tcttactgct agcttatcat atcaaaaaag aaggaaaaga
     7501 gatactatct tctctgtttg ttataagcaa tttttttgat aactctacct tattttttgt
     7561 tataatagtg gttatgagag tagtagcagg aacatttgga gggcgtcctt taaaaacgct
     7621 tgatggaaaa acaactcgtc caacaactga taaggttaaa ggcgctattt ttaatatgat
     7681 tggtcctttt tttgaaggtg gtcgtgtttt agaccttttt tctggcagtg gtagcttagc
     7741 tattgaggcg atctcaaggg gaatggacca agctgtccta gttgaaaaag ataggcgtgc
     7801 gcaggtcgtt attcaagaaa atattgcaat gactaagagt ccggagcaat ttcaattatt
     7861 aaaaatagaa gccaaccgtg ctttagaaca attaacggga caatttgatt tggtcttgtt
     7921 ggatccgcca tatgctaagg aagaaattgt aaagcaaatc caaatcatgg atagcaaggg
     7981 tttattaggc gatgatatca tgattgtttg tgaaacggac aaatcagttg atttacctga
     8041 agaaattgct tcttttggga tatggaaaca gaaaatttat ggcatttcta aagtaacggt
     8101 ctacgttcgt taggaggctt tagtatggat tacaagttag tgatgattat tctcatctta
     8161 gtcatattga tttatttgtt gttgcgcctt cactatttag aagagttgct aaaacattat
     8221 cacttcaaaa atgatgaagg agtttgttca ttatcaagaa caaaaaatcc attgtatcta
     8281 ttaatggcaa tgattacttt tttgccaaat cttatcaatt ggatagctaa cggttttgag
     8341 gcaatagaca ttgttcgcct gattcctgtg gtagtctttt tatggttgta ttattgtgga
     8401 gatgacgacg aatgacaaag aaagctttat ttacaggttc atttgatcct gttactaatg
     8461 gacatttaga tattattgag cgcgccagct atctatttga tcatgtttac attgggttat
     8521 ttcataacct tgagaagcaa ggttactttt cgattgagtg ccgtaaaaaa atgttagaag
     8581 aagctatccg tcagtttaag aatgtttcag ttttagtggc gcaagataga ctagctgttg
     8641 acttagcaag ggaagtggga gcaaaatatt ttgttcgagg tctgcgtaat agccaggatt
     8701 ttgattatga agccaatttg gaatttttta ataaacagtt ggcagatggt attgagacag
     8761 tttatttatc aacgtcgcca tctttgagtc cgatatcatc tagtcgcatc cgtgaattga
     8821 tacattttaa ggcttctgtg aagccttttg ttccaaagag tgtttttaga gaagtggaga
     8881 aaatgagtga agaataaaga tccaaaaaga aaacataaaa gtttgctagg tctgcttaaa
     8941 tggtggatta taggttttgc ttttttattg ttagtattag caagccttgt ggtgaggcta
     9001 ccttattatt tggaaatgcc aggtggagct tatgatatcc gttcggtatt gaaagtgaat
     9061 aaaaaggctg ataaagctaa gggttcgtac aattttgtag cggtatctgt cagtcaagca
     9121 acgccagctc aggttcttta tgcttggtta actcctttta cagaattatc tagtaaagag
     9181 gagacaacag gtggctttag taatgacgat tatctcagaa ttaatcaatt ctacatggag
     9241 acttctcaaa atgagtccat ctatcaagct ttaaagttag ctaataagca agtatctctt
     9301 acttataaag gagtttatgt gcttaactta gctaaaaatt cgacatttaa agatagatta
     9361 catttagctg atacagtcac aggagttaat ggcaaaagtt tcaaaaattc ttctcaactt
     9421 atcaaatatg ttgcagctct tcatctaggt gataaagtaa aggtacaata cacaagtcaa
     9481 ggtaaaaaga aagaatcagt tggtaaagtt attaaactat caaacggtaa aaatggtatt
     9541 ggtatcggtt tgacagacca tacagaagta tcatctgatg ttcctgtgga ctttaatact
     9601 gaaggtgttg gaggaccaag tgcaggttta atgtttactt tagcgattta cgaccaatta
     9661 gttaaagaag atttacgtaa aggacgtaag attgcaggca ctggtactat tgagcaaaat
     9721 gggcatgttg gcgatattgg tggagcaggt ttgaaagttg tttcagcggc taaaaaagga
     9781 atggatattt tctttgttcc taataatccg atcgataaaa atgctaaaaa aggcaaaaca
     9841 aaggttcaga cgaattatca agaggcaaaa gcagcagcta agcgattagg tacgaagatg
     9901 aaaattgtac cagttcaaaa tgttcagcaa gctattgatt atttgaaaaa aacaaaataa
     9961 actcaaagag ttcgggcgtc tgctcggctt ttttgttttg cgacatttaa ctatcaaagt
    10021 gctaaaatga tagtatgaca gagcttataa gaatactaca tttaaatgat ctgcactctc
    10081 attttgagaa ttttcctaag gtaaaacgat tttttcataa caatcaagcg cagccaatag
    10141 agacgatttc acttgacttg ggggataata ttgataaaag ccatcctttg acagaagcaa
    10201 gctcgggcaa ggctaatgtc caattgatga atgaactggg tattgagctt gctactattg
    10261 gtaataatga aggagttggt ttatccaaaa aagacttgga ccaagtctac aaagattcag
    10321 attttacggt tattgttggt aatctaaaag ataacataat cgaaccgagt tgggcaaaac
    10381 cttacattat ttatgagaca cagcaaggaa ctaagcttgc ttttttagct tatacttttc
    10441 cttattataa aacctatgaa cctaatggat ggacgataga agatccaatt gactgtctta
    10501 aatgccattt acaaataaat gagataaaag aggcaaattg tcgtattttg atgagtcatt
    10561 taggtattcg ttttgataca aggattgctc aagaattttc agaaattgac cttattatag
    10621 gtgcacatac acaccattta tttgaggaag gggagctaat taccggtaca tatcttgcag
    10681 cagctggtaa atatggtcgc tttgttggga gtattgatat aacttttgat aatcatacac
    10741 taaaagatat tttaatctca acatatgata caaaacaact aactggttat ccttcggaca
    10801 gtgattgggc taaggagatt gagtcagaag gtaaagaaca gcttagaaaa gaaagtttaa
    10861 tatcctttcc agcaccatta tcattagaag aatcgtgtca actagtcatg gatgcgatgc
    10921 tgttttacgc aggtgcagat gttgctatta tcaattctgg cttaattgtt cagccatttg
    10981 aaaaagactt ttcgaggaaa aatctgcatg aatcattacc tcatcaaatg cgtctcgcta
    11041 aattgacagt gagtagtcaa gaacttttag agatttatga aactatttat caacaaggtc
    11101 aatttttagc gcaacaaaag attcatggta tgggctttcg tggcaagtac tttggagaag
    11161 ttttacattc gggatttgat tacaaaaatg gaaaaatagt gtataatgag aaagatattg
    11221 atgccaaaga agaagtgatt cttgtaatcg ttgatcagta ttattttgca tcgtattttg
    11281 agtgtttaaa aacaaaaaaa gtagacttgc tttttcctga attactcaga gatgtggtag
    11341 cagattattt aataaatatg acactataaa tgaaaaatag ataaataaag agtagaagct
    11401 agtcgtttct ctcaatgatt ttagagaaag gagtattatg cgaaaagaag tgacaccaga
    11461 gatgcttaac tataataagt atcctggccc acagtttatt cactttgaaa atatcgttaa
    11521 aagtgatgat attgaatttc aacttgttat taatgaaaaa tcagcttttg atgtgactgt
    11581 ctttggacaa cgtttttctg agattttatt aaaatatgat tttatcgttg gcgattgggg
    11641 taacgagcag ttgaggctaa gaggctttta caaagatgct agtacgatta gaaaaaatag
    11701 ccggatttca cgtttagaag attatattaa agagtattgt aactttggtt gtgcttattt
    11761 tgtgttggag aatccaaatc ctagagatat taaatttgat gatgaaagac ctcataagcg
    11821 tcgtaagtca agatccaaat cacaatcatc aaagtcacaa actagaaata atcgttccca
    11881 gtcaaatgcc aatgctcatt ttacaagtaa aaagcgtaaa gacacaaaac gccgtcaaga
    11941 acgtcatatt aaagaagagc aagataagga aatgacctct gcaaagcagc attttgttat
    12001 tcgtaagaaa aaataaaaaa aggataaact gtaatcgttt attccaccat gaagaaagaa
    12061 aatattatgc ctaaaaaaac tgatacaccc gactataaac cctctattta tagcttaact
    12121 cgcgatgaac taatagcttg ggctattgaa catggagaaa agaaattccg tgcttcacaa
    12181 atctgggact ggctctataa aaagcgtgtg caaagcttcg atgaaatgac taacatttcc
    12241 aaagatttta ttgcactttt gaatgagaat tttgttgtca atcctctaaa acaacgtatt
    12301 gttcaagaat ctgctgatgg aactgttaaa tacctttttg aattacctga tggcatgctg
    12361 attgagacgg ttttgatgcg tcaatattat ggtttatcag tatgtgtaac gactcaagtt
    12421 ggttgtaaca ttggttgcac tttctgtgca agtggtttaa taaagaaaca acgtgatctt
    12481 aacaatggcg aaatcactgc tcaaattatg cttgttcaaa aatattttga tgaacgtgga
    12541 caaggtgagc gcgttagtca cattgtggtc atgggaatag gtgaaccctt tgataactat
    12601 accaatgtat taaaattctt gcgtacagtt aatgatgata atggattagc tattggtgca
    12661 cgtcacatta cagtgtctac ttcaggttta gcacataaaa ttcgtgaatt tgcaaatgaa
    12721 ggtgtacaag ttaacttagc agtatctctt catgctccaa ataatgaatt acgatcaagt
    12781 atcatgcgta ttaatcgttc tttccctctt gaaaagttgt ttgcggctat tgaatattat
    12841 attgagacga ctaaccgtcg tgtaacattt gagtatatta tgttaaatgg tgtcaacgat
    12901 accccagaaa atgctcaaga gttagcagat ttaacgaaaa agattcgcaa attatcttat
    12961 gtcaatttaa ttccttacaa tcctgtctca gaacatgacc agtatagtcg tagtcctaaa
    13021 gagcgtgtgg aagctttcta tgacgttttg aagaaaaatg gagttaattg tgttgttcgt
    13081 caagaacatg gtacggacat tgatgcagct tgcggacaat tacgttcaaa cacaatgaaa
    13141 cgcgatcgtc aaaaagcgaa ggtagggcgg taatgcttaa aagattattt actgaagatg
    13201 gggaattgac aaagattagt cgtcgtttcg tttggatgtt agtggttatc tattgtctta
    13261 ttattgtcag gatgtgtttt gggcctcaaa ttatgattga gggggtatca actccgaatg
    13321 ttcagcgctt cggaagaatt gtagctcttt tagtaccatt taattctttt cgtagtttag
    13381 atcagctaac tagctttaaa gagatttttt gggttattgg tcaaaatgta gtgaatattt
    13441 tactgctgtt tcctctcatt atagggttac tatccctaaa gccaagttta cggaaatata
    13501 aaagcgttat attacttgct ttcttgatgt ctcttttcat agagtgtact caagttgttt
    13561 tagatatttt aatagatgct aatcgggttt ttgaaatcga cgatctatgg acaaatacct
    13621 taggcggtcc tttcgcccta tggagttatc gaaacataaa aggttggctt ctaactatta
    13681 gaaaatgaaa ataatgtaaa ggctatgata ttgttcaagc cttttactat ttaaaaagga
    13741 tcactttagt tgacgaaagt aagcttatag aggcataata gagagttgtt aaatgagaat
    13801 tttaaaacac tttttatgtc ttctgattga tttggtcttc tgacttaatt atgatgtttt
    13861 tcaacctttg agaaaggaaa aatatgtcta ttataaaaaa tttgtggtgg ttttttaagg
    13921 aagaaaagaa gcgctatctt attgggattt tgtcattaag tttggttgca gtgttaaatc
    13981 ttatccctcc taaaatcatg ggatcagtta ttgatgctat tacaactgga aaattaacaa
    14041 gaccacaatt actatggaat ttattaggtt tggttttgtc agctttagct atgtatgggc
    14101 tgcgttatat ttggcgtatg tatattttag ggacttctta caaattaggc caagttgtca
    14161 gataccgttt atttgaacat tttacaaaaa tgtctccttc tttttatcag aaatatcgta
    14221 caggtgattt aatggcgcac gcgaccaacg acatcaattc tctaacacgt cttgcaggag
    14281 gaggagttat gtcagcagtg gatgcctcta tcacagcatt agtaacgctt atcaccatgt
    14341 tctttactat ttcgtggcaa atgacattaa ttgcggttat ccctttgccc ttaatggcct
    14401 tagcaactag taaattgggg cgaaaaaccc atgaaacctt caaagaatct caggcagcct
    14461 tttcagaatt aaataataaa gtgcaagaaa gtgtctctgg cgtcaaagtg actaaatcat
    14521 ttggttatca agaacaagaa attgcttctt ttcaagagat taatcagatg actttcgtga
    14581 agaacatgcg gaccatgact tatgatgtca tgtttgatcc tttagttctt ctttttatag
    14641 gtgcctccta cgtattaaca ttggctatgg gagcttttat gatttcaaaa ggtcaagtta
    14701 ctgttggtga cttggtaaca tttgtgacgt atttagatat gttggtatgg cccttgatgg
    14761 cgattggttt cttgttcaat atggtacagc gtggtagtgt ttcttataac cgtattaata
    14821 gtctacttga gcaagaatcg gatataactg atcctttaaa tcctatcaaa cctgttgtca
    14881 atggaacatt aagatatgat attgatttct ttagatacga caatgaggaa accttagccg
    14941 atattcattt caccttagaa aaaggtcaaa ccttaggttt ggtaggtcaa acgggatcag
    15001 ggaagacaag tcttattaag ttattgctac gtgaacatga tgtgactcag gggaaaatta
    15061 ctttaaataa acatgatata cgtgattatc gattgtctga gttacgtcaa ctaatcggtt
    15121 atgttcctca agatcagttt ttatttgcta ccagtatttt agaaaatgtt cgctttggaa
    15181 atccaactct atctatcaat gctgtcaaag aagcaactaa attggcacat gtttacgatg
    15241 acattgaaca gatgccagca ggatttgaga ctctaattgg agaaaaagga gtctcattat
    15301 ctggtggaca aaaacaaagg attgcgatga gtcgtgccat gattttagat ccagatattc
    15361 ttattttgga tgattctcta tcagcagtgg acgctaaaac ggaacatgct attgttgaga
    15421 atcttaaaac gaatcgtcaa gggaaatcga ctattatttc agcacatcgt ttatcagctg
    15481 ttgtgcacgc agaccttatc ttagttatgc gagacggcag agtcattgag cgaggtcaac
    15541 atcaagagtt gctaaataaa ggtggttggt atgctgaaac gtatgcctca cagcaattag
    15601 aaatggagga agcatttgat gaagtctaat caatggcaag tctttaagag attaatctcc
    15661 tatttacgcc cttataaatg gtttacagta ttagctctat ctctcttatt gttgacgact
    15721 gttgttaaaa atattattcc tttaattgct tcacatttta ttgatcacta tctgacaaat
    15781 gttaatcaaa cagcagttct tattttagtg ggatattatt caatgtatgt cttgcagacc
    15841 ttaattcaat attttgggaa tctctttttt gcgcgtgttt cttatagtat tgttagagat
    15901 attcgtagag atgcttttgc taatatggaa aggctaggca tgtcttattt tgataggaca
    15961 ccggcaggat ctattgtgtc acgtattact aatgatactg aagcaatatc tgatatgttt
    16021 tcgggtattt tatcaagttt tatctcggcg atatttattt ttacagttac tctgtacact
    16081 atgttgatgc tagacattaa actaacagga ctcgtcgctc ttttgttacc tgttatcttt
    16141 atattagtga atgtctatcg gaaaaaatca gtcactgtca ttgctaaaac gagaagttta
    16201 cttagtgata tcaacagtaa attatcagaa agtattgaag gaattcgcat tgtacaggct
    16261 tttggtcaag aagagcgctt gaagactgaa tttgaggaaa ttaacaaaga gcatgttgtg
    16321 tatgccaatc gttctatggc tcttgatagt ctcttcttaa gaccggcgat gtctctttta
    16381 aaactcctag catatgctgt tcttatgtct tattttggat ttacaggagt taaaggaggt
    16441 cttacggcag gattaatgta tgcttttatt cagtacgtta atcgtctatt tgacccttta
    16501 attgaagtaa cgcaaaattt ttcaacctta caaacatcaa tggtatcagc agggcgtgtg
    16561 tttgatctga ttgatgaaac aggttttgaa ccaagccaaa aaaatacaga agcttttgtg
    16621 agagaaggaa atatcgaatt taaaaatgta tcattctcat atgatggaaa aaaacaaatc
    16681 cttgataatg tttcttttag tgttaaaaaa ggtgaaacta ttgcttttgt aggagcgaca
    16741 ggttctggaa aatcatctat tattaatgtc tttatgaggt tttacgaatt tcaatctgga
    16801 caagttttat tagatgggaa ggatattagg gattactcac aagaacaatt gcgaaagaat
    16861 attggactcg tcttacaaga ccccttttta tatcatggca ctattaaatc taatatcaaa
    16921 atgtaccaag acatcaccga tcaagaggta caggacgcag cagagtttgt tgatgctgat
    16981 caatttattc agaagttgcc agataagtac gattcagctg tttcagaacg tgggtcaagt
    17041 ttttcaactg gacaacgcca actattagcg tttgccagaa ctgtggctag taaacctaaa
    17101 attttaatct tagatgaggc gacagcaaat attgattcgg aaacagagca gattgtacaa
    17161 gattcgttag caaaaatgcg tcaaggtcga acaacaattg ccattgctca ccgcttatca
    17221 accattcaag atgctaattg tatctatgtt cttgatagag gaaagataat tgaaagtggg
    17281 aatcacgagt ctttattaga ccaaaaaggg acttattatc gtatgtacca attacaagcc
    17341 ggcatgatgg aggtctagaa aatttaggct attcaaaagg cataatctgt gaggcttaga
    17401 ggttggaata gttattcctc taagcctttt ttatatttta caattagtgt ataatgaggt
    17461 caaagtacaa ggaaaggtct ataaaatgtt actattggta gataattacg attcttttac
    17521 ctataattta aagcaatatt taagtgtcta taaagaaata ttcgtcataa aaaatgatga
    17581 tcctaacctt tttttattag ctgagagcgc agaagctatc gtcttatcac ctggaccagg
    17641 acacccaaag gatgctggga aaatattaga attaatcaat cagtttatag gacagaaacc
    17701 tattctaggt atctgtttgg ggcatcaagc gcttgctgag tgcttgggtg ggcgattaaa
    17761 tttagcaaat cacgttatgc atggtaaaca aagttgggta acaataaacg accatactag
    17821 tttgtttaag ggaattgata gtccaaccca agttatgcgt tatcactcat tggtagtgac
    17881 tgatttacca gagaatattg ctattattgc acgatcaaat gaggataatg aaatcatggc
    17941 attccattgc cccagcttaa aggtatatgc gatgcagttt catcctgagt caatcgggag
    18001 tattgatgga atgaaaatga ttgaaaactt tttgacgtta attaatgact aaaatgtgtt
    18061 agtgtaggat aatgactctc tataaataaa agacagtacc taagggcact gtcttttatt
    18121 tagttgtata gatgagttaa tctgtctgca aatttacgat aaataatcaa gcaaatggat
    18181 gcttttaagg tatccccagg aataaaaata aggttagaaa atagtgattt agttaagggg
    18241 agagaagttt gaaaggaaag aacgatgcta ccacaaatat caatcaatag tacgccaaat
    18301 atccaaataa taaggaatta aacccagagt tttgttgttt tcactttctc aagccctagg
    18361 ccgataaaaa aagctgcaaa tggataagtc agcagatagc ctgctgttgg cccaaacagc
    18421 gttacgagac ctgaccgccc tcctggtaaa atggtgcacc aatagcaact aagagcaaga
    18481 aaatagccac agctaggaaa cctttcctac taccaagcag agcacctgcc agcataacac
    18541 caagattttg taagacaatt gggactggaa tgaaacctaa tgggatccct gggataaaac
    18601 ctaaaacaat gagtagtgca accatgagtg caatataggt agttgttctt gttttcattt
    18661 aaattaattc ctttttagta tgatttagtt gtaatttttc caagaattca atatcggtat
    18721 gttgattgcg acccccctct gttaggtaat ttcccaaaaa agttgagtca acaagtaagg
    18781 tgactaatga ttcaaaattc tcaaaatgga cctcccgtcc agcacttaat cttaattcct
    18841 tgaaaggaaa aacaaaacgc aacatggcca taatttttaa gcatttaatg ggagtcaaat
    18901 agttatattt tccaagaggt gttccttcaa cagcaagtaa aaaattgaca gggatagaat
    18961 aggggttcag ttcttttagt ctgaaagcta atgtgatgag cccctcatcg ctctctccca
    19021 taccacaaat gaatccagaa caaacatcaa tgtcttcatt gtgaattctt tctaaagtat
    19081 cacaacgatc tttgaagcta tgcgtcgttg caatgttggg gtagttttct tcgggggtgt
    19141 tgaggttatg attgatacgg tcaattcctg cttgttttag ctgatgaagt tgttctctat
    19201 ccaaaaaacc agcggtaagg catatttcta gaggaaggct ttttttgatt tcagggatga
    19261 cttgacataa ctgctcaatt tctctcttgc taggcttagt tccactcatc gcaatacaaa
    19321 agacgctagc accattttga tgagcgacaa tagcctgttt tagaatgaca ttttgaggaa
    19381 gcagaccaaa tttatcaatg tcagcagtgc tttctttaga ttgagaacag taaccacaat
    19441 tttctgcaca aagtccttgc ttagcactta ataaaacgtt aaggcgaata ttatttcggc
    19501 taacttgatt tttaaggtat aaggcagcag cgtagatttc ccaccagttc tcatcgctat
    19561 ttagaatttc cagcgcttgt tcataagaaa tgctagtctt tccagataat atttcgtcag
    19621 ccaaatggat ataatttgtt tgaaatgaca tgatagcctc ctttaacgaa attaataata
    19681 gcataaaaaa actgtattgt aaacttagtc tatataataa gttaacaata taaaaaatta
    19741 tgatatactg gtcttatatt ttgacgggag ataagatgaa aaaactattt aaaaaagcga
    19801 tagctatgtg ccgagatttg ccgcttaatt ttgtaattgt tgaacttagt ttgtatcaaa
    19861 gagaggatgg ctcatctgat aatattaaat ggatttctaa gcgaatcctt gaggtaacgg
    19921 taactgatca gcaatggatt taccatttga ggatgcttaa tttaccaaaa cttaagccaa
    19981 tgtcactagc atccagtgac tgtcctgcaa gggaagagaa ggctagtctt atttcaatag
    20041 aagaatgtca aaaattcact agcttagtgc gagataaaaa tccttttcac caaggggaag
    20101 cagctattgt gcctgcggct ctattattgg aaaaaataat ggaagttaga aatggaaaaa
    20161 taaaggagat tcatttgact tttttatctc cagtaagggt gaatcaagaa ttctcagtgc
    20221 aagtagtgga taacacagtg attatttttc agcatcaaat attatgtgtg agggggacat
    20281 atgaagaata gagatgttta tattggtttt gggctacgaa cacctatcgg tataaaaggg
    20341 aagcaattta aacattaccg tccagaactt ttaggagcac acctcttaaa tcaaataaaa
    20401 aaaatagaat cagaatctaa tattgatagt attatttgtg ggaacacagt tggtactggg
    20461 ggcaatattg gtcgtttgat gactcttttt tctgattatg aatcctatat tccagtacaa
    20521 acgattgata tgcagtgtgc ttcatcaagt tctgccttgt tttttggtta tctaaaaatc
    20581 agtgccggta ttaatgaaaa agttcttgtt ggggggattg aaagtagttc tcttcaacct
    20641 atgagacgtt acgctaaaga agataatcgt aacggagaat ataccgttgc tcagttttct
    20701 cctgactctt atgctgaaac tgtaatgtta gaaggggcac aaagagtctg tcaaaaatat
    20761 ggttttagaa gagaaatgtt agataaattg gcattcttga gccataaacg cgccttaaca
    20821 gctaaacaag gtggctattt agaagaggta atcttaccaa tggaagggat gcgagatcaa
    20881 ggcgttagaa aactaaaaga agcatttttt caaaaattac caagattgat ggaaaattca
    20941 cctttgctca ctattggaaa tgtttgttta atgcacgatg ctgctgcatt tctaacgctt
    21001 cagagtcaga aaacagaatt ccggattgtt cacattgtag aagtagcagg agatcccaag
    21061 cttagtccag aattggttca cacggctacg gaaaaactat taacagaaac tcatactaaa
    21121 atatcggatt atgatgcaat tgaatggaat gaaccatttg cagcgattga tgctttattt
    21181 aatcattatt atcctgaaga gagagaaaaa ttcaatattt ttggaggggc attagcttac
    21241 ggacaccctt atgcctgctc aggaattatt aatatccttc atcttatgca ggcattaaaa
    21301 tataaaaata aacctatggg tctaactgcc attgcagggg caggaggagt cggaatggcc
    21361 atatcaatag agtacttagg agttaagaat gcttgaatca ttgaaaacaa tagttaaaac
    21421 caatagcgat aaaaagttat ttgatggtga tttacaggtc agttatggag aattttataa
    21481 cttagtcaga caagatatgg cgtcacaaga caataggaaa catgtgatta gcacacactc
    21541 tctgcttaac caattagtaa gatttgtttc aaagttatgt caaaaagctc ttcctattat
    21601 ttgtaaacct aaccttactc ataatgaaat aagtcgatta gaaaaagaag tgcagtatgc
    21661 tcctcaacta gctgattttg gtgttctttc ttctgggact acagctgatg ctaaactatt
    21721 gtggaggagc ttcgcttcgt ggtctgattt ttttagcatt cagaatgctt atttttcggt
    21781 cacttccaat agcagactgt ttattcaagg tgatttttca tttactggta acttaaattt
    21841 agcgctatcc cttttattac tggggggaac gcttgttgtc acacagaaaa attctgtaaa
    21901 atactggcag actttatggg aaaaaactgg tgtaacgcat ctatatctgc tccctagtta
    21961 tctcaagtta gtggaacaat atagcaagga gactgcgtta gacaataaaa ctattattac
    22021 ctcttcgcaa tatgtctctg actcattact ggaaggttta tatataaagc atcctaaagt
    22081 gtcagtgaaa atcttctatg gtgctagtga actcaattat gtgtcatggt acgatggtcg
    22141 tgatatccga gataagccac agtatgttgg agaaatcgtt ccaaatgtag ctgttaggat
    22201 taaagaggga agaatttttg taaaaacacc gtatagtatc tgtggccttt caagtgaata
    22261 ttgtgctggt gattatggtg aactgataga tggcaaatta tatctatttg gtcgtggtga
    22321 cgattggtgc aatcaatcgg gcattaagct ctatttacct agattaattg aaaaaataaa
    22381 aacatgccct tatatcaagg atgcagtagc atttacaaaa gagagtcaaa gtcatggaca
    22441 agagtcacat tgctgtattg ttttaattga aaatcagatg caacaagagt gtttgaagtg
    22501 gttatcggaa cattttgaaa agaagtatgg gtttaagcat tatcatattg tatctaaaat
    22561 tcctttgatg ccatctggaa aaattgatta tcaacaactc aaaagacaat tagcatgagt
    22621 ttttactaaa aaaataaaat agggttaatg gattgcaaac ctcagttctg atattcttta
    22681 ttttgaaaga tatcgtgata taatgataaa aaagaaagat aaggaataaa atgttatcaa
    22741 aagcaaaatc acgatatatt atccgtgaga ttattaagtt atttccagat gctaaaccta
    22801 gcttagactt tactaatgtc tttgagcttt tggtagctgt aatgctatca gctcaaacaa
    22861 ctgatgcagc tgttaataaa gtcactcccg ccctttttga acgctttcca aatcctttag
    22921 ttttggcaca agctgatccc aaagagattg aaccctatat ttctaaaatt gggctttacc
    22981 gtaataaagc tcgattctta aatcaatgcg ctaaacaatt gatagagcat tttgatggga
    23041 aagtccccca aactcgtcaa gagctagaaa gtttgtctgg cgttggaagg aaaacggcta
    23101 atgtggtcat gagtgttgga tttggtattc cggcttttgc cgtagatact catgtcactc
    23161 ggatttgtaa acaccaccag atttgcaaac agtcggcttc tccactagaa attgagaaac
    23221 gagtcatgga ggttttaccg ccagaagagt ggctagctgc tcatcaatcc atgatttatt
    23281 ttggtcgtgc aatttgtcat cctaaaaatc caaaatgcga ccagtaccca caactttatc
    23341 actttcctga taatttaaag tgattagaat aaaagagagt aactggtgac gattatataa
    23401 gcgatactta aaaacggtag aaaagctatc gtttttttca tttttctcat aactaaacaa
    23461 taaataattc ctaaccaagc acctatttga ataataaaaa gcatttgatg aagaggtaaa
    23521 gataagccaa ttgtggctaa ataaaggaaa tctccacttc cgatattaat atcaaccact
    23581 tcagctaaaa tcccaaataa acataaaaca atagaaactt ttgtaatcgg tg
//
LOCUS       DATRNX010000021        23560 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRNX010000021 DATRNX010000000
VERSION     DATRNX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23560
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..262
                     /locus_tag="VBC81_000665"
     CDS             <1..262
                     /locus_tag="VBC81_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911723.1"
                     /translation="VTLYIYRETIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGE
                     KVSPTTFIIPISVVVIILISLGFIVNHQLKKLNMLDALKSVD"
     gene            368..1246
                     /locus_tag="VBC81_000666"
     CDS             368..1246
                     /locus_tag="VBC81_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6911724.1"
                     /translation="MRKSIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            1353..1943
                     /locus_tag="VBC81_000667"
     CDS             1353..1943
                     /locus_tag="VBC81_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6911725.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1977..3248
                     /locus_tag="VBC81_000668"
     CDS             1977..3248
                     /locus_tag="VBC81_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6911726.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            3261..3692
                     /locus_tag="VBC81_000669"
     CDS             3261..3692
                     /locus_tag="VBC81_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6911727.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3784..4668
                     /gene="truB"
                     /locus_tag="VBC81_000670"
     CDS             3784..4668
                     /gene="truB"
                     /locus_tag="VBC81_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6911728.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4681..5613
                     /locus_tag="VBC81_000671"
     CDS             4681..5613
                     /locus_tag="VBC81_000671"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6911729.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5656..6069
                     /locus_tag="VBC81_000672"
     CDS             5656..6069
                     /locus_tag="VBC81_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6911730.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            6062..6343
                     /locus_tag="VBC81_000673"
     CDS             6062..6343
                     /locus_tag="VBC81_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6911731.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            6333..7097
                     /locus_tag="VBC81_000674"
     CDS             6333..7097
                     /locus_tag="VBC81_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6911732.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            7165..8475
                     /locus_tag="VBC81_000675"
     CDS             7165..8475
                     /locus_tag="VBC81_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6911733.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8654..9520
                     /locus_tag="VBC81_000676"
     CDS             8654..9520
                     /locus_tag="VBC81_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6911734.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9566..10483
                     /gene="pstC"
                     /locus_tag="VBC81_000677"
     CDS             9566..10483
                     /gene="pstC"
                     /locus_tag="VBC81_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6911735.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10473..11360
                     /gene="pstA"
                     /locus_tag="VBC81_000678"
     CDS             10473..11360
                     /gene="pstA"
                     /locus_tag="VBC81_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6911736.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11372..12175
                     /gene="pstB"
                     /locus_tag="VBC81_000679"
     CDS             11372..12175
                     /gene="pstB"
                     /locus_tag="VBC81_000679"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6911737.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            12187..12945
                     /gene="pstB"
                     /locus_tag="VBC81_000680"
     CDS             12187..12945
                     /gene="pstB"
                     /locus_tag="VBC81_000680"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6911738.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12979..13632
                     /gene="phoU"
                     /locus_tag="VBC81_000681"
     CDS             12979..13632
                     /gene="phoU"
                     /locus_tag="VBC81_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6911739.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13778..16327
                     /locus_tag="VBC81_000682"
     CDS             13778..16327
                     /locus_tag="VBC81_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6911740.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16489..17169
                     /locus_tag="VBC81_000683"
     CDS             16489..17169
                     /locus_tag="VBC81_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911741.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            17153..18466
                     /locus_tag="VBC81_000684"
     CDS             17153..18466
                     /locus_tag="VBC81_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6911742.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18555..18887
                     /locus_tag="VBC81_000685"
     CDS             18555..18887
                     /locus_tag="VBC81_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6911743.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18905..20470
                     /gene="ffh"
                     /locus_tag="VBC81_000686"
     CDS             18905..20470
                     /gene="ffh"
                     /locus_tag="VBC81_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6911744.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20537..21223
                     /locus_tag="VBC81_000687"
     CDS             20537..21223
                     /locus_tag="VBC81_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6911745.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            21213..21986
                     /locus_tag="VBC81_000688"
     CDS             21213..21986
                     /locus_tag="VBC81_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6911746.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWVLLV"
     gene            complement(22019..23458)
                     /locus_tag="VBC81_000689"
     CDS             complement(22019..23458)
                     /locus_tag="VBC81_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6911747.1"
                     /translation="MDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSD
                     GLTYTATLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINS
                     GDIKDLNKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSK
                     NQIYSGPYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQ
                     IDFAEISGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPK
                     KLAKLPNGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNA
                     VDYVKSTWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFT
                     TNSAYNYGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGK
                     KGLQNSKLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         7973 a         3966 c         4324 g         7297 t
ORIGIN      
        1 agtaacttta tacatttacc gcgaaaccat tattttatca accatcggtg tgattctagg
       61 caccattagc ggtacttatt tacaccgtca aatgatgttg ctaattggtt cagatcaaat
      121 actttttggt gaaaaagtat caccaactac atttataata ccaataagcg tagtagtcat
      181 cattctaata agtctaggtt ttatagttaa ccatcaatta aaaaaactca atatgttaga
      241 tgccttgaaa tcagtagatt agtttataaa aaagattatc ttttctaatc ttttcgataa
      301 cgattgacag caagtaatct taattgtata atataacagt ataaagttag ataaaagtga
      361 gatatttatg agaaagtcta tttttgagga aactgcaaaa ggtaaagtat caaggcatgt
      421 ccttttaact atattattgg ctttgatatt actatttaca gcagatttcc ttacctatat
      481 tattattgaa ttgattatct cgcttgattt ttggaaacta gagtgggata gcgatatcgt
      541 ccagttaatc attttactag caacttcaat tagtacggta atgttattta tgtgggtgaa
      601 atttaaagag aagcgtccaa ttagttctct agggctattt aaagaaggag ccctaacaga
      661 attatctaga ggatggttag tcgggagcgt tctctttgga gcctgcttaa tattaacaat
      721 aatttcaggc tctggagtta tcgaaactgt tcgttttaca gctgataaat tgatttggtt
      781 tcttattttt gctgttggat ggcaggtaca aagcgcatct gttgaaatat tgtgtcgtgg
      841 atggttactt ccggtgagtg ccacaaagta tagtaagata gtttctgtca gtatcagttc
      901 aatctttttc ggtcttctgc atagcgctaa taatcatgta agccttatct cgattttcaa
      961 tctgtgtctc tttggactat ttctatcgct ctatgttatt ttaaaaggta acatctgggg
     1021 ggcgtgtggt attcatggcg cctggaactg tgtacaaggg agtgtctttg gaatcgaagt
     1081 tagtggagag ccaatgttgt ctaactccct agtccatgtt aaaacctatg gtgctgactg
     1141 gattagcggt ggaaaatttg gtgtggaagg aagcatgatt accagtattg ttcttatcgt
     1201 agcatgttat tggctttatc aaaaatcgaa ttttccttac gactaataga tttaatctaa
     1261 ataagcttct cttactgaaa gcttattttt tattttagta ttaaaaaact atgttttgtg
     1321 atataatatt ttaaagacga aaggagtcta ttgtgccaca aaatccaaca agtaacccag
     1381 agaaaaaaac ttatttatta tctgaattgg tagactgctt taaaggtaaa gctgtgcctt
     1441 caaaagcaga agcaggtgat atccgtatta ttaatttgag tgatatgtct cctttaggaa
     1501 ttgactacca taacttaaga acattccaag atgaacaacg atcgctttta aaatatctac
     1561 ttcaggaagg cgatgtttta atcgcttcaa aaggaactgt caagaaagtt gctatttttg
     1621 aagagcaaga ttaccctgtc gttgcctctg ctaatattac aatactaagg cctacacaac
     1681 acattagagg gtactactta aagctttttt ttgattcaga agaaggtcaa caggcacttg
     1741 agaatgctaa caaaggaaaa gctgtgatga atattagcac aaaggaactg ttaaatattg
     1801 caataccaag tatcccgctt tttagacagg attatcttat ccaacgttat aaacaggggc
     1861 tcaacgatta caaacgtaag atagcacgtg cagagcagga gtgggaacgt atccaaaatg
     1921 acatcagaca acaacttagc tagaaccaaa tacaaaacat cataggaaga gttatcatga
     1981 acgaaatcaa atgccctcat tgtggaacag cttttgccat caacgagtct gaataccatc
     2041 aattactaga acaaattcgt ggagatgctt ttgacaaaga agtaagtgaa cggttggaaa
     2101 aagaacgtct aatattaggg gagcaagcaa aaaatcaatt acaggaagtt gttgtagaaa
     2161 aagacaagga gatagctaaa cttcagtaca aagtcaaaca atttcttata gaaaaagaca
     2221 atcttctcaa agacaatgag taccaactcg ctgagcaatt aaatcaaaaa gacatgatgc
     2281 ttcgcgacct tgaaaaccaa atcgatagac tacgtttaga gcatgaaaat agcttgcaag
     2341 aggcgctaac aaaagtcgaa cgagaaagag atgcaataca aaatcagttg cacattcaag
     2401 aaaaagaaaa agatttagct ttagcttcag taaaaagtga ttatgaagta caactaaagg
     2461 cagccaatga acaagtagaa ttctataaaa acttcaaagc tcaacagtct actaaagcag
     2521 taggagaaag tttagaacat tatgctgaaa cagaatttaa taaagtgcga catttggcct
     2581 ttcctaatgc ttattttgag aaggacaata cattatcaag tcgtggctca aagggggact
     2641 ttatctatcg agaaaaggat gaaaatgacc ttgagttttt aagtatcatg tttgaaatga
     2701 aaaatgagtc tgatgatact atcaagaagc ataaaaatga agattttttc aaagaattag
     2761 ataaagatcg tcgtgaaaaa tcttgcgaat acgcagtttt agtaactatg cttgaagcag
     2821 acaatgacta ttataatact ggaattgttg atgttagtca caaataccct aaaatgtacg
     2881 ttatacgtcc acaatttttt atccaattaa ttggtattct aagaaatgca gcactcaata
     2941 ccttaaaata taaacaagag cttgctttga tgaaagaaca aaatattgac atcacacatt
     3001 ttgaagaaga tttagatatt ttcaaaaatg catttgctaa aaattataat tctgcaagca
     3061 aaaatttcca gaaagcaatc gatgaaatag ataaatctat taaacgtatg gaagctgtta
     3121 aggctgcttt aacaacgtct gaaaatcaac tacgtcttgc aaataataaa ttagacgatg
     3181 tttctgtcaa gaaattaaca agaaaaaatc caacaatgaa agcaaaattc gatgctctaa
     3241 aagactaaga ggttatgatt atgaagatta gaactgctac actagacgat agtgaaaaat
     3301 tagtcccttt gtatcaagaa ttaggatacg caatatcatt atcagagatt caatctattt
     3361 taaaggttat tcttactcat tcagattatg gctttttgat agctgaggat aatggcaaac
     3421 tactcgcctt cgttggatat cataaacttt acttttttga aaaaagtggg acttactatc
     3481 gtattttagc actagtagtt aatgaaaagc accgtcgtaa aggcatcgct agtcaactta
     3541 ttaatcacgt taaacagcta gctaaaacag atggaagtga agtactggct ttaaatagca
     3601 gtttaaaaga atatcgtcag gaagcttatc atttttatga aaacctaggg ttcaagaagg
     3661 ttagtacagg cttttcatac tatttagatt aggtataaat ccttatagca ctataccaaa
     3721 agagcgtaag tttttacgct cctttctttg tgttaccgct tatttactga tataatataa
     3781 aacatgatta caggaataat taatttaaag aaagaagctg gaatgacatc acacgatgct
     3841 gtattcaagc ttcgaaagat attacataca aaaaaaatag gacacggcgg aactcttgat
     3901 ccagatgttg ttggggtttt accaattgcg gttggaaaag ccactcgtgt tattgaatat
     3961 atgacagaaa gtggcaaaat ttatgaagga gaaatcactt taggatatgc cacaagtaca
     4021 gaagattcta gtggagaggt catttcaaga actccactaa ctcaatccga tcttagcgag
     4081 gatgttgtgg atcatgcgat gaaaagtttt accggtccaa ttacacaagt tcctcctatg
     4141 tattcagcag ttaaagtaaa tggtaaaaaa ctctatgaat atgctcgttc aggagaagaa
     4201 gtagagcgac ctaaacgaca aataacaatt tctgaattta gacgcacaag cccattgtac
     4261 ttcgaaaaag gcatctgccg ttttagtttc tatgtctcat gcagtaaagg tacctatgtt
     4321 aggacgttag ctgttgatct aggaataaaa ctaggatatg ctagccatat gtctttccta
     4381 aaaagaacct catctgcagg attatctatt acacaatcat taacgcttga agaaattaat
     4441 gaaaaatata agcaagaaga tttttcattt ttacttccaa ttgaatatgg tgttttagat
     4501 ctcccaaaag ttaacctaac tgaggaagat aaagttgaaa tctcatacgg acgtcgtatc
     4561 ttattagaaa atgaagcaga taccttagca gctttttatg aaaatagagt tatcgctata
     4621 ctagaaaaac gtggcaatga atttaaacca cacaaagtat tgttataaag aaagtcaatc
     4681 atgaaaatcc attacattaa tgattataaa gatattcaag ctaaagaaga ttgcgtctta
     4741 gttttgggct attttgatgg cttacatctg ggacataaag ccctctttga taaagctaaa
     4801 aaaatcgcga ccgaaaaaaa tttgaaaatt gtcgtcttaa cgtttaatga gacacctcgt
     4861 ctaaccttcg ctcgcttcca acctgaatta ttgctacatc ttacctctcc agaaaaacgt
     4921 tctgaaaaat ttcaagagta tggcgtcgat gagttatatt taatgaattt taccagtcat
     4981 ttttcaaaag tatcttctga cctttttatt aagaaatata tttatggtct cagagcaaaa
     5041 gctgctgttg tcggttttga ttataagttc ggtcataatc gtaccagtgg tgattatcta
     5101 gcgcgtaatt tcaagggacc agtctatatt attgatgaaa tttctgaagg tggtgaaaaa
     5161 atctcttcta cacgtattcg tcaattgatt acagagggta atgttgagaa agctaaccaa
     5221 cttttgggtt atgagttttc aacctgtggc atggtagtac atggagatgc tagaggacga
     5281 actatagggt tcccaactgc taatctagct cctattaatc gcacttactt acctgcagac
     5341 ggtgtttata tttccaatgt tctgattaat ggaaaatatt atagagcaat gacgagcatt
     5401 ggaaaaaata tcacttttgg tggtacagaa cttcgtttag aagctaatat ctttgatttt
     5461 gatggcgaca tttatggaga aactattgaa attttctggt taaaaagaat cagagaaatg
     5521 gtaaagttta atggtattga cgatcttgta aaacaactta aaaaagataa agaaattgct
     5581 ttaaattgga aaaaagatag tcaaactctt taattttttg tataatagtc tctagtaaat
     5641 acaagaggcg gttatatgat taccttattt ttatcaccca gctgtactag ttgtcgaaaa
     5701 gcgcgtgctt ggctaagtaa gcatgaggtt gcttttgaag agcataacat tattacaagt
     5761 ccactcaata aagaagaact attacaaatt ctatcgttta cagaaaatgg tacagaagac
     5821 attatttcca cacgttcaaa agtctttcaa aaacttgcta ttgatgtaga cgaattatca
     5881 acgtcttcat taatggaatt aatttcagaa aatccaagtc ttttacgacg cccaattatt
     5941 ttagacaaaa aacgtatgca gattggtttt aatgaggatg aaattcgtgc cttcttaccg
     6001 cgtgactatc gtaaacaaga actgaaacag gccactattc gtgctgaaat tgagggaaaa
     6061 catgattagt agtaattata gttatcctct tgatccttca tggaatacgg aagatattac
     6121 taaggtactt cgctttttaa atcaagttga acacgcctat gaaaatagtg ttaaagttga
     6181 tgatttgcta gatagctata aagaatttaa aaaatttgtt aaatccaagg cgcaggaaaa
     6241 acaaattgac cgtgaattcc agagaactag cggttattcc acttatcaag ctgttaaagc
     6301 agcacaacaa caagcgaaag gatttatttc acttggacgc taaatttgat ttcgctaaac
     6361 aattagtgta taaagctggt caattcatta aatctgagat gcaaaatact tttgatgttg
     6421 aggaaaagtc tcgatttgat gatttagtga caagtcttga taaaaaaaca caaaaattat
     6481 taattcaaga aattattcaa cattacccag atgataatat acttgccgaa gaagatgatg
     6541 tgcgctcacc cattgcacaa ggtaatgttt gggttttaga ccctattgat ggaactgtta
     6601 attttattgt tcaaaaagat aactttgctg ttatgttagc ttattacgaa gaaggcgttg
     6661 gacaatttgg tatcatctac gacgttatgg ctgatattct ttacagcggg ggaggacatt
     6721 ttgatgtcta tgctaacgat aaaaaaattg ttccttttca agagtgtcct ttagaacgtt
     6781 gcttacttgg agtaaattct gctatgtatg cagaaaatga ttgcggtatt gcccatttag
     6841 cttctgaaac cttaggtgtt cgtatctatg gtggtgctgg aattagcatg gcaaaagtca
     6901 tgcagggcaa attattagct tatttttcgt atattcaacc ttgggattat gctgcagcaa
     6961 aaatcatggg ggagacatta ggatttacct tattaacgct tgacggggaa gaacccaact
     7021 attcaacaag acaaaaagta atgttcctac caaagtcgaa attaaatctt atccaatcct
     7081 accttaccaa aaaataaact taaaaccttc taattaaata gaaggttttt ttgcacgccc
     7141 gtgattatgt agtataatag aaacatgaaa ttaccgaatg aatttattga aaaatatcag
     7201 actatactta aagatgaagc agaagctttt tttgacagtt ttgaacaaaa acccatatcg
     7261 gcatatcgca ccaatcctct caaagaaaag caacttgatt ttccaaatgc catcccaagt
     7321 accccttggg ggcattatgg gaaaatatca ggaaaatcaa tagaacacac aactggcctt
     7381 gtttattctc aagaacctgc tgctcagata gtggctcaga ttgctgaacc acaagaagga
     7441 atgaaagtgc tagatttagc agctgcacct ggtggaaaaa caacacacct tctatcatat
     7501 ttaaacaata ctggtctatt agttagtaat gaaatttcaa ataaacgtag taaaatttta
     7561 gtcgaaaatg tcgaacgttt tggtgctaga aatgttatcg tgactaatga aagctcccaa
     7621 cgactagcca aatgttttaa ttcttttttt gatcttattg tttttgacgg tccgtgttct
     7681 ggtgagggaa tgttccgcaa agatcctcaa gcaatacaat attggcacaa agattacccc
     7741 actgaatgtg cacagttaca aagagatatt ttaaaagaag caatcaaaat gctagctcat
     7801 ggtggtatac ttgtatactc tacttgtaca tggtcaccag aagagaacga agaagtggtt
     7861 aattggctcc ttcaagagta tgattactta gaacttgttg atattcctaa actaaatggg
     7921 atggttgaag ggattaatgt accacaagtt gcaaggatgt accctcatca ttttcaaggt
     7981 gaaggacaat tcgttgcaaa actgagagat actagatcta aggaagcaca gaaaattaag
     8041 ccaaaagcac agaaaataaa taaaatgcag ttacaattgt ggcaacaatt tgcacaagac
     8101 catttaaaga tagacttgaa cggtgtttta gatgttttcg gcgaccaact ctatcttcta
     8161 cctaatggtc tccctgattt gtctaaatta aaaatagcac gcaatggtct ccatctaggc
     8221 actttcaaga agaatcgttt tgagccatcg tttgctttag gaatggcact tagcgaacat
     8281 gacctagtac agtctattga aattgatata gaacagtttg aggtgtacgt atctggaaat
     8341 gtagtcaaac tagacaagac tgttccaaac ggttggtatc aaattcttgt aaaaggcaat
     8401 ggattaggtt ttgcaaaagt gacaaataat actcttaaaa attattaccc taaaggacta
     8461 cgatttcaga cataactgtt attgaatact tttaataaaa aaggaaaaag aaatactttt
     8521 caaaccctag aaacttatgg tactatggga cagtgatgtg tgtttttact atttgtaaat
     8581 cattaaacac tcaatgattt tagtaatata tatcaactac taaatatcaa aatttattca
     8641 agagaatagg aaaatgaaaa tgaaaaaaaa catttttttg ttaaccttgc tagccaccgt
     8701 cttattgacg ctgtctggtt gtgctaattg gattgataaa ggtcaatcaa tcaccagcgt
     8761 tggttcaacc gccttacagc cacttgtaga agctgcagca gatgagtttg gtaagacaaa
     8821 cctcggtaag actatcaatg tccaaggagg aggctcagga acaggcttat cacaagtaca
     8881 gtctggagct gttcaaattg gtaatagcga cttatttgca gaagaaaaag atggtattga
     8941 tgctaagaaa ttgaaagacc atcaagtcgc agtagctgga ttagctgtta ttgttaataa
     9001 aaaagttaat gttaaaaatc taaccaccca ccaacttcgt gatatttttg caggaaaaat
     9061 caagaattgg aaagaagtgg gaggacaaga tttagatatt tccatcatca atcgagcagc
     9121 aagttctgga tcccgtgcaa ccttcgataa tactattatg ggaaatgttg ctcctattca
     9181 aagccaagaa caagattcaa atggtatggt gaaatcaatt gtttctcaaa cacctggtgc
     9241 gatttcatac ctcgcttttg catacgttga taaatctgtc ggaacattaa aactaaatgg
     9301 ttttgcgcca acagctaaaa atgttactac cgacaactgg aaactttggt catatgaaca
     9361 tatgtataca aaaggtaatg aaacaggtct aacaaaagaa ttcttagact acatgaaaag
     9421 tgataaagtt caaagtagta tcgtacaaca tatgggatat atctctatta acgacatgaa
     9481 agttgttaaa gatgctgaag gtaaagtaac cccaaaataa aacttaatca catctagata
     9541 aagcatcttg atcctaggag gaattatgaa aaatcaagaa ttagctaaaa aattaacatc
     9601 tccttcaaaa aactctcgat tagaaaaatt tggtaaagga ataacttttc tttcgcttgc
     9661 cctaatcgtc ttcattgttg cgatgatttt gatttttgtt gctcaaaaag gactatctac
     9721 attttttgtt gatggcgtta aattaacaga cttcttattt aatacaaaat gggaaccaag
     9781 tgctaagtca ttcggtgctc ttccaatgat agctggatca tttattgtta ctattctatc
     9841 agctatcatt gctacaccat ttgctatcgg tgctgccgta tttatgactg aaatctcacc
     9901 taaatatggt tctaaaattt tacaacctgc agttgaatta ctagttggga ttccttctgt
     9961 tgtttacggt tttattggtt tacagattat tgttcccttc gttcgctcta tttttggggg
    10021 aacaggtttt ggcattttat ctggggtctg tgtcctcttc gtcatgattc taccaactgt
    10081 tactttcatg acagtagata gccttagagc tgtgccacgt cactataaag aagcaagttt
    10141 agccatgggg gcaactcgtt ggcaaacaat ttggcgtgtt atattaaacg ctgcacgtcc
    10201 aggaattttc actgcaattg tttttggtat ggcacgtgca tttggtgaag ctctagcaat
    10261 tcagatggtt gtcggaaact ctgcaatttt accaacatca ttgacaacac cagcagcaac
    10321 attaacgtct gttctaacaa tgggtattgg taatactgta atgggaactg tacaaaacaa
    10381 tgtcctctgg tcgctagcct tagtactttt aattatgagt ttagcattta atacagttat
    10441 taagttaatt acaagggaag gaaagaaaaa ctatgaacgc taaaaaggct gacaaattag
    10501 caactacaat actctatagt attgcagcta tcatcgttac aatcctcgcc tctcttttaa
    10561 tttttattct tgttcgcgga ttgccacacg tatcttggtc atttttaact gggaaatcat
    10621 catcatatga agcaggaggc ggtattggaa tacaattata caattccttt ttcctactta
    10681 ttgttactct gattatttct attcctctat ctttaggagc tggaatatac ctctcagaat
    10741 atgctaaaaa aggacgttta acaaactttg ttagaacatg tatcgaaatt ttgtcatctc
    10801 ttccttctgt tgtagttggt ctgtttggtt acctaatctt cgttgttcaa tttcaatatg
    10861 gcttctcaat tatctcaggt gccttagcac ttactgtttt taacttaccc caaatgacac
    10921 gtagtgttga agatagcctt caaaatgtac accacacaca gcgtgaagca ggattggccc
    10981 taggtatttc tcgttgggaa acggtcattt acgtcgttgt ccctgaagct ctgccaagta
    11041 ttgtaactgg tgttgtgcta gcttcaggac gtattttcgg tgaggcagca gcacttattt
    11101 atacagctgg tcaatctgct ccagcacttg attggtcaaa ttggaacgtc cttagtgtta
    11161 caagtccaat ctcaattttc cgtcaagcag aaactttagc tgttcatatt tggaaagtta
    11221 atagcgaagg gacaatccct gatgcaacac aagtatctgc cggtagcgcc gcagtcttat
    11281 tggttgttat tcttattttc aacctttcag ctcgctcgat cggtaagaaa cttcattcga
    11341 aattaacctc gtctaattaa aaggagtagt aatggcagaa tataattggg acgaacgcca
    11401 tatcattacc ttccctgaag aaaattcagc tctcaccaca aaagatttac acgtttacta
    11461 cggtgagaaa gaagctatca agggaattga tatgcaattt gaaaaaaata aaattacagc
    11521 cttaatcggc ccatctggat gtggaaaatc aacatatctt cgtagcttaa atcgtatgaa
    11581 cgatacgatt gatattgcac gcgtgactgg acaaatcatg tatgaaggaa ttgatgttaa
    11641 cgctcaagat atcaatgttt acgagatgcg aaaacatatc ggaatggttt tccaacgtcc
    11701 aaacccgttt gcaaaatcaa tttataaaaa cattaccttt gcatatgagc gtgctggtgt
    11761 aaaagataaa aaattcttag atgaagttgt cgaaacctca ctaaaacaag ctgctctatg
    11821 ggatcaagta aaagatgacc ttcataaatc agcattcact ttatctggcg gtcaacagca
    11881 gcgattatgt attgcacgtg ctatcgcagt taaaccagaa attctcttaa tggatgaacc
    11941 tgcttcagca ctcgatccga ttgcaactat gcaactagaa gagacaatgt ttgaactcaa
    12001 gaaaaattac actataatta tcgtaacaca taatatgcaa caagcagcac gtgctagtga
    12061 ttatactgca tttttctatc ttggagactt aatcgaatac gataaaacga ataacatttt
    12121 ccaaaatgct aaatgtcaat ctactagcga ttatgtttct ggtcgatttg gatagtgagg
    12181 taaaagatga cacaacctat tttacaagtc agtgatttgt ctgtttacta taataagaaa
    12241 aaagctttaa aagaagtctc aatggacttc tatcctaatg aaattacagc cttaattggt
    12301 ccgtctggtt ctggaaagtc aacactctta cgagctatta accgtatggg agacttaaat
    12361 cctgaagtca ctctaacagg agcagtgatg tataacggac ataatgttta tagcccgcgc
    12421 actgataccg tagaacttcg taaagaaatt gggatggttt ttcagcagcc taatccattt
    12481 cctatgtcag tatttgaaaa tgttgtttat ggtcttagac tcaaaggaat aaaagacaaa
    12541 gctacgttag atgaagccgt agagacttca ttgaagggcg cgtctatctg ggacgaggtc
    12601 aaagatagat tacacgactc tgctcttggt ttatctggtg gtcaacaaca acgtgtttgt
    12661 attgccagaa cccttgctac caagcctaaa attatccttc tagatgagcc aacctctgca
    12721 cttgacccaa tttctgctgg taaaattgaa gagactttac atggtctaaa agatcaatat
    12781 accatgttac ttgttacacg ttctatgcag caagcctcac gtatttctga tcgtacaggt
    12841 ttcttcttag acggtaactt aattgaatat ggcaatacaa aagaaatgtt tatgaatcct
    12901 aaacataaag aaactgaaga ttacattaca ggtaaattcg gttaatacca tataataaga
    12961 tcagaaagag aatacaatat gttaagatca aaatttgatg aagaattaga taaactgcat
    13021 aaccaattct atgcaatggg aattgaagct atcggacaaa tcaaaaaaac agttcgtgct
    13081 tttgtcagtc atgatcgaga gttagctaaa gaagtgatag aagatgatgt aacgcttaac
    13141 aactttgaaa cgaagttaga gaaaaaatca ttggaaatta ttgcactcca gcagcctgta
    13201 tcacaagact tacgcaccgt tattaccgtt ttaaaagcta ctagtgatgt tgagcgtatg
    13261 ggagaccatg cagcagcagt tgcaaaagca accatccgta tgaagggcga ggaaagaatc
    13321 ccagcagtag aattagaaat caataatatg ggaaaagcag ttaaaaacat gcttgaagaa
    13381 gccttaacag cttatatcaa tggtgatgat gaaaaagctt acgaagtagc agcaatggat
    13441 gaaatagttg atgattactt ccgtgatatc caaaaaatgg tagttgaaac aattcaaaag
    13501 catcctgacg tagcatttgc cgccaaagaa tacttccaag tattgatgca cttggagcga
    13561 attggagatt atggcaaaaa tatctgtgaa tggattgttt atttaaaaac tggaaaaatt
    13621 atcgagttat gatataatta ttgcttgtag agtggtaatt gtaagtatag ttcgtgcgat
    13681 agaaaagctg taactatctt gttacagctt ttctatcttt tctagcccat acaggactag
    13741 taatgtttca aatgtttctg aattagttag gagttatatg aaaactgtag aacattttgt
    13801 taccaaattt gtccctgaaa actataacct tttcttggat attaatcgtc aaactaaaac
    13861 cttttcaggt aacgttgctg tgtctggcga agccttagat aacaatatct cttttcacca
    13921 aaaagggtta accattaaat ctgtcttatt agataatcaa ccattagatt tccaactaga
    13981 tgaagacaat gaagctatgc atattcaatt gcatgaaact ggtagcatgg tattagtctt
    14041 tgaattcagc ggtcacatta ccgataatat gacaggtatg tatccatctt actatacagt
    14101 taatggcatc aaaaaagagg ttatctccac acaatttgaa agtcattttg ctcgtgaagt
    14161 attcccaagt attgacgaac cagaagcaaa agcaaccttt gatttgagcc taaaattcga
    14221 tcaaaaagaa ggagagattg ctctttcaaa tatgccggaa atcaatgctg aacaacgtca
    14281 agaaacaggt ttatggacat ttgacaccac acctaaaatg tcctcatatt tattagcttt
    14341 tgccttgggg gaattgcatg gtaaaacaac acataccaaa aacggcactc ttgttggctc
    14401 atatgccaca aaagcccatc agttaaatga attagatttt tctttagata tcgttgtgcg
    14461 tgttattgag ttttatgagg actactttgg tgtacgttat cctattcctc agtcactcca
    14521 cgttgctcta cctgattttt ctgctggagc tatggaaaat tggggattag taacatatcg
    14581 tgaagtctat cttttagtag atgagaactc tagtgtttcc agccgtcagc aagtagcatt
    14641 agttgtagca catgaaatag ctcaccagtg gtttggtaac ctcgttacta tgaaatggtg
    14701 ggatgattta tggttaaatg aatcatttgc taatatgatg gaatatgtat caattgatta
    14761 tattgaacca aaacttaata tatttgaaga ctttcaaaca ggtggtctac cattagctct
    14821 taagcgtgat gccacagatg gcgtccaatc tgttcacgtt gaggtcaatc atcctgatga
    14881 aattaacaca ctttttgatc cagctatcgt ttatgcaaaa ggtagccgcc tcatgcacat
    14941 gttacgtcgc tggttaggcg atacagattt tgccgcagga ctaaaaattt attttgaaaa
    15001 acatcaatat caaaatacta ttggtcgtga cctttggaat gcactttctc aaacctcagg
    15061 aaaagatgtt gcagctttca tggattcttg gttagagcaa ccaggatacc ctgtgatggc
    15121 agccaaaata gaagaagatg agctcatttt aactcaaaaa caattcttta ttggtgaaca
    15181 cgaagataag tctcgcttgt ggcaaattcc tcttaatagt aattgggaag gaatcccaga
    15241 gatattaacc gaggaaacgg tagtaatccc caattttagt cagttagctg aaaagaacaa
    15301 agaaaatggt gcactgcgtt tcaatactga aaatactgca cattacatta ctaactatca
    15361 aggtcaactc ttagagcata tcatttctga tcttcctctt atggataaca tttctaaact
    15421 acaaattgta caagaacgtc atttacttgc tgaaagtggt atgatttcat atagtagttt
    15481 aataccttta gtctctcttt tgagccaaga gacatcatat cttgttaact ctgctattaa
    15541 gagtgttatt gatggtttat ccttattcgt ccaggaagat agccaagatg aattcgactt
    15601 taaagaattt gttaataaat tatcagcatt taattttaat agacttggct ttgaaaaaag
    15661 agaaggagaa ggagatgata gcgaaatggt ccgtcatcta tccctaagcc ttgctctcta
    15721 ctcagataat gaacatgcta ttgaagaagc tcaccatatt tttaaagccc acgaaaataa
    15781 tatcgctgct ataccagctg caattcgcct gttagttctt accaatgaaa tgaagcattt
    15841 cgaatccaaa gaacttagcc acttgttatt agagacttat agtactacta ccgatgggaa
    15901 tttcaaacga caactagcga gtgctttatc tcatactaca gatagcaaga cactcaagaa
    15961 attattgtca gattggaaaa ataaagatat tgtgaaacca caagacttag ctatgagttg
    16021 gtatgctact ttccttaaaa attcttttac acaagaaagt gtctgggagt gggcccaaga
    16081 aaactgggaa tggatcaaag caactctagg tggtgatatg agttttgata aatttgtgat
    16141 ttatccctca agtagtttca aaacagaaga acgccttgaa caatataaaa actttttcga
    16201 gcctcaatta agcgatatgg cgattagccg taatatttca atgggaatta aagaaatttc
    16261 tgctcgtgtc cttttaatca cgaagcaaaa agaagaagtt atcaatacta tcaaaaaata
    16321 tatctaataa actcaaatag agattgatgg attattcaat ctctatttct ttgtctgtgt
    16381 catgaaatta gttgacataa aacatacttt atttaggatt tattaagcaa agtttggtat
    16441 tctaatatta atagttcaga taaaatatgt acatcgaaag gatatactat gataaaaatt
    16501 ttattaattg aagatgactt gagtctttcc aattccgttt ttgatttcct tgatgacttt
    16561 gccgatgtca tgcaaatttt tgatggagaa gagggtcttt acgaagccga aagtggagtc
    16621 tatgatctga ttcttctaga tctcatgtta cctgaaaaaa acggcttcca agtgttgaaa
    16681 gagctacgtg aaaaaggtat tacaacccct gtattaataa tgaccgctaa agaaagcatt
    16741 gatgataagg gacaaggttt cgacttaggt gctgatgatt atctaacaaa acctttttac
    16801 ctagaagaac taaaaatgcg aattcaggct cttttaaagc gttctggaaa atttaatgat
    16861 aacagcctaa tttatggtga tattcgcgta gacatgtcta ctaactcaac ctttgttaat
    16921 caaacagaag ttgagctctt aggtaaagag tttgatcttt tagtctattt ccttcaaaat
    16981 caaaatgtta tcttaccgaa atctcaaatc tttgatcgta tttggggatt tgatagtgat
    17041 acaacgattt cagttgtaga agtctatgtc tctaaggttc ggaaaaaatt aaaaggaaca
    17101 cttttttcag aaaaccttca aactttgcgt agtgtaggat atattttaaa gcatgtcgaa
    17161 accgattaag ataagtaaat tcaaaaaaaa tgtctcagat agtcatttta tccatttttt
    17221 cacagttttt agtggtattt ttctggttat gactgttatc atcctccaag tcatgcgcta
    17281 tggtgtttat tcttctgttg atagtagttt aaagtatatc agtacccatc ctaaaaatta
    17341 catcaatatg gttatgtcac gtactgctgc ctacctagat aactccaata tcgcaagtgt
    17401 caagttaaaa cccggcggtc agacagtagc taatactgat attattcttt ttacatcaga
    17461 agaagaagtc attaactatt ttgacgcatt ttcaaactat cagtttttaa aacctaataa
    17521 aaaaaatcta ggtggcatta gtgaattaac tttaaccaac atctttggtc aagatgagac
    17581 atatcatgct gtcaccgtaa aagttaataa ccctgcttat cctaatgtga cttacatgac
    17641 agctattgtt aatattgatc aattagtaaa tgctaaagaa cgctatgaaa aaattattat
    17701 atttgtcatg actacttttt ggattatttc aattggagca agtatctact tagctaaatg
    17761 ggcacaaaaa ccgattatag aaaactacga aagacagaag gcttttgtcg aaaatgctag
    17821 ccacgagctg cgtactcccc tagctgtctt acaaaatcgc ctagaaacgc tattcagaaa
    17881 accgaatgcc accattttag aaaatagtga aaatatcgct tcaagtctag atgaagtacg
    17941 aaatatgcgt attttaacca ctaatctttt aaacttagca agacgtgatg atggaatcaa
    18001 accagaatta gctgttatta agcctacact ttttgatagt atctttgaga attatgatct
    18061 aatcgcccaa gagaatggta aaaattttac tggacataat atgatccaag atagtttcaa
    18121 aacagataag acactgctaa aacaactcat gactatttta tttgataatg ctataaaata
    18181 tactgacaat gatggtagca ttgactttac catatctgaa actgataagt atctcttttt
    18241 agaaatcgct gataatggac caggaatttc agaggaggat aaagtcagaa tttttgatcg
    18301 tttttaccgt gttgacaaag caagaacacg acaacaagga gggtttggcc tcggcctttc
    18361 tttagctcaa caaatcgtta actcactgcg aggtaatatt acagtaattg ataataaacc
    18421 tagaggcagt atttttaaga taaaactacc aaaagtaaaa ggttaggcca tgttagtcct
    18481 aaccttttaa attttaaagt ggtcttgcat acttgatata gcaagctgat aggaaatttg
    18541 gtataataaa agctatggaa attgaaaaaa caaatcgtat gaatgccctt tttgagtttt
    18601 atgcggcatt attaacagat aaacaaatga attatatcga gttatactac gccgatgact
    18661 acagccttgc tgaaatagct gaagaatccg gtgttagccg acaagctgtc tatgataata
    18721 tcaaacgaac tgaaaaaatt cttgaggcgt acgaaatgaa actacatatg tattcagatt
    18781 atattgtccg tagtcagatt tttgatgaca ttctagaaaa atataccgat gatgcctttt
    18841 tacaagaaaa aatttccatt ctaagtagca ttgataatag agattaaatg aggagaaata
    18901 atttatggct ttcgaaagtt taacagagcg cctgcaagga gtttttaaaa atattcgtgg
    18961 taagaaaaag ttatctgaaa aagatgtgca agaagtcacc aaggagattc gtctagctct
    19021 tttagaagct gacgttgctc ttccagttgt taaaaccttt attaaacatg ttcgtgaacg
    19081 tgctgttgga catgaaatca ttgatacact agatcctacg caacaaattg ttaagattgt
    19141 caatgaagag ctaacagatt tattaggggc tgagacctct gaaattgaaa aatcccctaa
    19201 aattccaacc atcattatga tggttggtct acaaggtgct ggtaaaacaa cttttgccgg
    19261 taagttagca aacaaactaa tcaaagaaga caatgctcgt cctatgatga tagcagctga
    19321 tatttatcga ccagctgcta ttgatcagtt aaaaaccctt ggtagccaga ttaatgttcc
    19381 tgtctttgat atgggaacta accattcagc agttgagatt gtcactaaag gtttagaaca
    19441 ggctcgtgaa aatcgtaatg actatgttct tattgataca gccggacgcc tacaaattga
    19501 tgcaaccctt atgcaggaat tgcatgatgt caaagccata gctcaaccta atgaaatact
    19561 ccttgtcgtt gacagcatga ttggacagga agctgcaaat gtcgctgaag aatttaaccg
    19621 tcagctcagc attagtggtg ttgttctgac aaagattgac ggtgatacac gcggtggtgc
    19681 tgccctttca gtacgcgaaa ttactggaaa accaattaaa tttacaggga caggggaaaa
    19741 aatcactgat attgaaactt tccatcccga tcgtatggca agccgtatat tagggatggg
    19801 agacctgcta acacttattg agcgcgctag tcaagaatac gacgaaaaac gttcaatgga
    19861 attagcggag aaaatgcgtg aaaacacctt tgacttcaac gattttattg atcaattaga
    19921 tcaagttcaa aatatggggc caatggaaga tctccttaaa atgcttccag gaatggctaa
    19981 caatccagca atgaaaaact tcaaagtgga tgaaaacgaa attgctcgca aaagagccat
    20041 tgtgtcatca atgactccag aagagagaga aaatccaaat ctcctcaatc caagtcgtag
    20101 acgtcgtatt gctgctggat caggtaatac tttcgttgat gttaataaat tcattaaaga
    20161 ttttaaccag gcaaaacaaa tgatgcaagg cgtgatgtcc ggtgatatga ataaaatgat
    20221 gaaaaaaatg ggaattgatc ctaataacct ccctaaagat atgcctggaa tggacggaat
    20281 ggatatgtct aatttagaag gtatgatggg gcaaaatggc atgcctgact tatctagttt
    20341 aggtggcgat atggacttca gccaaatgtt tggtggtggc ttaaaaggaa aagtaggagc
    20401 ctttgcagct aaacaatcaa tgaaacgtat ggctaataaa atgaagaaag ctaagaagaa
    20461 acgcaaataa aaatgtctat ttaaatagac atttttattt ttgtctgcta taatagacaa
    20521 aaaggtggtg atatttatgt atttagcatt aatcggtgat atcattaatt caaaacagat
    20581 acttgaacgt gaaactttcc aacagtcttt tcagcaacta atgaccgaac tatctgatgt
    20641 atatggtgaa gagctgattt ctccattcac tattacagct ggtgatgaat ttcaagcttt
    20701 attgaaacaa tcaaaaaagg tatttcaaat tattgaccat attcaactag ctctaaaacc
    20761 tgttaatgta aggttcggcc tcggtacagg aaacattata acatccatca attcaaatga
    20821 aagtatcggt gctgatggtc ctgcctactg gcatgctcgc tcagctatta atcatataca
    20881 tgataaaaat gattatggaa cagttcaagt agctatttgc cttgatgatg aagaccaaaa
    20941 ccttgaatta acactaaata gtctcatttc agctggtgat tttatcaagt caaaatggac
    21001 tacaaaccat tttcaaatgc ttgagcactt aatacttcaa gataattatc aagaacaatt
    21061 tcaacatcaa aagttagccc aactggaaaa tattgaacct agtgcgctga ctaaacgcct
    21121 taaagcaagc ggtctgaaga tttacttaag aacgagaaca caggcagccg atctattagt
    21181 taaaagttgc actcaaacta aagggggaag ctatgatttc tgatttttta agggataatc
    21241 caatcctaac tttattattt tgcgcacatt ttttagctga ctttcagtgg caaagtcaat
    21301 ccttagcaga tagtaaatca catagttgga ggggactctg gagacatctc ttgattgttt
    21361 tcctccccct agcagcttta atgatattga taccagaaac tactttgtta aatttaagca
    21421 tatggggaag tcatattgtt attgatagta taaagaaatt gagttaccct tgggtagagg
    21481 aagggcattt ccaaaaagca gcttttatta tagaccaact agcccactat acttgcatta
    21541 tcgtttttta ccatgcgctt cctacatatt taccgcctaa tcattggctt ttgcctatca
    21601 agcactttat tgtcatagct ctggttttta tcattatcac caaacccatt aatattgtgt
    21661 ttaaaatttt ctttaataaa tttcaagcta aagaattatc atcattatta acacaagaaa
    21721 aaacaaaaat aatgaaagaa aaatctgaag atcatgaaga aacaattgaa ggagcaggag
    21781 ctatgattgg taatttagag cgtctaatta tggctatatt actaatctca ggacagtatg
    21841 ctgctatagg tcttgttttt actgcaaaat ccattgcacg ttacgataaa atttctaaaa
    21901 gtcaggtttt tgccgaatat tatttaattg gatcattgtt ctcaattata agtgttttga
    21961 taactcattg ggtacttttg gtatagaaaa gcgctagaaa tatcctaacg cttttctttt
    22021 attctgtctt ataagcataa gtaaaatcaa cattcaaacc agttgaattt cttaccaacc
    22081 cctttaattt agaattttgg agtccttttt tccctaggta ataaactggg ttataatatg
    22141 attgatcaaa taaagctttt tcagcagttt tgtaatcatt agcggcatct cctggtttca
    22201 aggcgtcagt agtaattgcc ttttgatagg cattgtcata ctctttagaa gaaaatttac
    22261 cataattata cgcagaatta gtcgtgaata acccatagaa agttgatcct tctggataat
    22321 ctcctcccca tgagaagagc acgacatcaa aattttcatt ttttgcatct tcaaggcgtt
    22381 gcttgaaagt aacaaatttt tcttcaacag ttagaccagg caaagcactc tcccatgtag
    22441 atttcacata gtcaacagca tttttagctg ctggagagtc cgaatcagct gtgattgtta
    22501 acttgagaga tgactgacca acctcagcaa gaccttcttt aaaaagtttt tgagctttac
    22561 ttgtattata cgtataacct ggggcagtat acttggaaag atcctcacca ttaggtaatt
    22621 tggctaattt tttagggacc agagattcag caggagttga acctgtatca actgccgctt
    22681 taacaacacc tttacgatca gtcgctaaat tcaaagcttg cctaattttc tgatttgtca
    22741 atgctttaac cgaaccagtt tggttataaa tagcagaagt tcctgatatt tcagcaaaat
    22801 caatctgacc ttgcttgtac atttgaactg cagtatctgg ttttttgata gtttgaacta
    22861 tgacactatt tgttttaaca tgtttagaat cccaataata tttatttttg actaatttaa
    22921 atttaccatt tgaaccattc caatccttaa caaggtaagg acctgagtaa atttgatttt
    22981 ttgatgttgt accataatct ttaccaactt tttcaacata cgattgtttt tgaggcataa
    23041 aattagagaa ggctaaataa tatttaaatt gagggcatgg acttgtcaat ttaaaggtca
    23101 cttgattgcc tttagctgtt acaccaagtt tatttaaatc tttaatatct ccagaattaa
    23161 tcttatcagc atttaaaagg tgcgattctg tcgccaagta agcatattca gatgcagttt
    23221 taggatcaac tattctccgc catgtgtaaa caaaatcttc agcgcttagt ttgcttccat
    23281 ctgaccattt taaattatca cgaagtgtag cagtataagt aagaccatca gaagaaacgg
    23341 atactttttt agctaaatcg ggttttggtt tgccctcttt atcaattcta agtaaattgc
    23401 tgcctgaatt acctatagct aagtttgaat aagcatctgt attctttgaa atgtccaatg
    23461 tactgatttc agtaggaata taccaattaa taactttatt atctccagag ttctttgact
    23521 cattaccaca ggcaaccagg gtaactgtgg tggctgttag
//
LOCUS       DATRNX010000022        23492 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRNX010000022 DATRNX010000000
VERSION     DATRNX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23492
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(68..658)
                     /locus_tag="VBC81_000690"
     CDS             complement(68..658)
                     /locus_tag="VBC81_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6911748.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(783..1412)
                     /gene="upp"
                     /locus_tag="VBC81_000691"
     CDS             complement(783..1412)
                     /gene="upp"
                     /locus_tag="VBC81_000691"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6911749.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1522..2691)
                     /locus_tag="VBC81_000692"
     CDS             complement(1522..2691)
                     /locus_tag="VBC81_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6911750.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(2827..3375)
                     /locus_tag="VBC81_000693"
     CDS             complement(2827..3375)
                     /locus_tag="VBC81_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6911751.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(3548..4900)
                     /locus_tag="VBC81_000694"
     CDS             complement(3548..4900)
                     /locus_tag="VBC81_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6911752.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            5106..6455
                     /gene="trkA"
                     /locus_tag="VBC81_000695"
     CDS             5106..6455
                     /gene="trkA"
                     /locus_tag="VBC81_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6911753.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            6460..7899
                     /locus_tag="VBC81_000696"
     CDS             6460..7899
                     /locus_tag="VBC81_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6911754.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(7923..8174)
                     /gene="yidD"
                     /locus_tag="VBC81_000697"
     CDS             complement(7923..8174)
                     /gene="yidD"
                     /locus_tag="VBC81_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6911755.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(8174..8896)
                     /locus_tag="VBC81_000698"
     CDS             complement(8174..8896)
                     /locus_tag="VBC81_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6911756.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(8886..9470)
                     /locus_tag="VBC81_000699"
     CDS             complement(8886..9470)
                     /locus_tag="VBC81_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6911757.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(9467..10174)
                     /locus_tag="VBC81_000700"
     CDS             complement(9467..10174)
                     /locus_tag="VBC81_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6911758.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(10174..10914)
                     /gene="xerD"
                     /locus_tag="VBC81_000701"
     CDS             complement(10174..10914)
                     /gene="xerD"
                     /locus_tag="VBC81_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6911759.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(10904..11377)
                     /locus_tag="VBC81_000702"
     CDS             complement(10904..11377)
                     /locus_tag="VBC81_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6911760.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(11374..11895)
                     /locus_tag="VBC81_000703"
     CDS             complement(11374..11895)
                     /locus_tag="VBC81_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6911761.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(11877..12851)
                     /locus_tag="VBC81_000704"
     CDS             complement(11877..12851)
                     /locus_tag="VBC81_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6911762.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(12848..13642)
                     /gene="racE"
                     /locus_tag="VBC81_000705"
     CDS             complement(12848..13642)
                     /gene="racE"
                     /locus_tag="VBC81_000705"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6911763.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(13817..14056)
                     /locus_tag="VBC81_000706"
     CDS             complement(13817..14056)
                     /locus_tag="VBC81_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6911764.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(14214..14756)
                     /locus_tag="VBC81_000707"
     CDS             complement(14214..14756)
                     /locus_tag="VBC81_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911765.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(14818..15339)
                     /locus_tag="VBC81_000708"
     CDS             complement(14818..15339)
                     /locus_tag="VBC81_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6911766.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(15407..16096)
                     /locus_tag="VBC81_000709"
     CDS             complement(15407..16096)
                     /locus_tag="VBC81_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6911767.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(16119..16622)
                     /locus_tag="VBC81_000710"
     CDS             complement(16119..16622)
                     /locus_tag="VBC81_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6911768.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(16660..17403)
                     /locus_tag="VBC81_000711"
     CDS             complement(16660..17403)
                     /locus_tag="VBC81_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6911769.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            17435..17713
                     /locus_tag="VBC81_000712"
     CDS             17435..17713
                     /locus_tag="VBC81_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6911770.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            17798..18730
                     /gene="yidC"
                     /locus_tag="VBC81_000713"
     CDS             17798..18730
                     /gene="yidC"
                     /locus_tag="VBC81_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6911771.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(18930..19595)
                     /locus_tag="VBC81_000714"
     CDS             complement(18930..19595)
                     /locus_tag="VBC81_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6911772.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(19619..20476)
                     /locus_tag="VBC81_000715"
     CDS             complement(19619..20476)
                     /locus_tag="VBC81_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6911773.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(20637..22097)
                     /locus_tag="VBC81_000716"
     CDS             complement(20637..22097)
                     /locus_tag="VBC81_000716"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6911774.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(22201..22683)
                     /gene="greA"
                     /locus_tag="VBC81_000717"
     CDS             complement(22201..22683)
                     /gene="greA"
                     /locus_tag="VBC81_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6911775.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(22756..>23492)
                     /gene="mltG"
                     /locus_tag="VBC81_000718"
     CDS             complement(22756..>23492)
                     /gene="mltG"
                     /locus_tag="VBC81_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6911776.1"
                     /translation="TIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDETFIKKMVAK
                     YPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKIAPYYNTIK
                     AKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAILYAMGKLGD
                     KTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDYLYFVADVK
                     TGNVYYAKDFETHKANVEKYINSQIN"
BASE COUNT         7527 a         4541 c         3673 g         7751 t
ORIGIN      
        1 tactaagatt ataacataat tccaacaaat aaaaaaagag ttggtaaact caactctttt
       61 aaatctatta ttgtaaatta ttattttcca taatagcatc aataaagcca taatcaagtg
      121 tttcttgagc actcatccaa cgatcacgct ctgcatcatc atggactttt tcaatagatt
      181 gaccagaatt atcagctaag attttttcta aagtatgacg cgtttttaaa agatgctcag
      241 cagcgatagc catatcagat tgctgtgtac ctccgcctgt tccgcccatt ggttggtgga
      301 tcatatattc tgcattcggt aacataaaac gttttccttt agcaccactt gaagcaataa
      361 tggttcccat cgaagcagcc atccccataa caatcgtctg tacgtccgat ttaatgaagt
      421 tcatggtgtc cacaatagca agtccagccg atactgaacc acctggtgta ttgacataaa
      481 ggtaaatatc ctttgtatta tcttgtgcat cgagaaacaa taactgtgca atgatactat
      541 tggccatatt atcctcaact tggcctgtca acataataat acgatctttt aaaagacgtg
      601 agtaaatatc ataagaacgt tcaccacgac ttgtttgttc aataactact ggaatcatat
      661 ctttctccat ttctttttat cactgtcact attataatca tttggtcaac aaaggtcaaa
      721 taataacttt tttaagaaaa ggctttctga gttcttcaga aaagccttat tcatctcaaa
      781 tcttattttg taccaaataa gcgatcccca gcatctccga gtccaggaac aatgtagcca
      841 tgttcattga gtttttcatc caaagcagcg gtataaatat ctacatctgg atgtgcttct
      901 tgaagtgctg ccactccttc tggagctgct actaaacaaa cgaatttgat actagctgcg
      961 ccacgttttt ttagagaatc tactgctaaa atagcagacc cacctgtagc aagcattgga
     1021 tcaactacaa aaatctgacg ttgatcaata tcttcaggta acttaactaa atattcgact
     1081 ggttggaaag tctcttcatc acgatacata ccgatatgac caactttagc agctggcaca
     1141 aggcttaaga aaccatcaac cataccaata cctgcacgta aaattggaac aattgctaac
     1201 tttttcccag ctaattgttt ttgtacagtt gtcgctactg gtgtttggat ttcaacatct
     1261 tctaaaggca aatcacgaga cacttcatag cccattaaca tcgcaatctc atcaactaac
     1321 tcacgaaaat ctttagtaga tgttgtagtg cgacgtaaga ttgacagttt atgttgaata
     1381 agtggatgtg aaatgacttg aaattttccc attataatga ccgctttcta tttaattttg
     1441 agcattaaaa acattctaat gcttgtttac attcaaagta ttataacata aaaacccttt
     1501 ccatgcgact ttttttatag tctagtcatt ttttctaaag tttgtcacta atcgcaaaac
     1561 tgcctcctca ataacagacc taggagctgc gacattgatt ctagcatgtt ttttcccttc
     1621 ttttccaaaa gttaaaccat catttaatat taatttggca tcatatcgaa gtttttcttg
     1681 aatttctaaa tgcgttaaat tataagcaga aaaatctaac caaacaagat aagttccctc
     1741 tggtttcatc actttgatat gagttttttg cgttaattgc tcataaaggt attctattga
     1801 tccttctaac tccattttta gggcctttaa ccattgtttc ccttttgtaa aggcaacttc
     1861 tgtagctaat aaccccaatg aagaaatttc ttgttgattg ttagctattt gcctcttctt
     1921 aaaatccgag cgtaactttt cattttcaat tattgcaaaa ctatttttag tccctgcaat
     1981 attaaacgtt ttagttgcac tagataaaat aacactcaat tctttaaaac tagaatcaac
     2041 agtattaaag ctatggtgta cattatcaaa taataccaaa tcttgatgta tttcgtcaga
     2101 aacgaggata acattatatc gtttacagat atctcctatt ttttgaattt ctccttttgt
     2161 ccagacacgc ccaccgggat tgtgtggact acaaaaaata taaagcttta cattattttc
     2221 aatgatatct ttttctaatt gtttaaagtc aatctcaaaa tactgattat tattaagtaa
     2281 actatttgaa actaaatgac gattattgta ttttatagtt ctagcaaagg gcggatagac
     2341 tggggtatta attagaacag catctccctc ctttgtgaag gcttgtatag caactgaaat
     2401 cgctggtaca accccatcaa ttaataataa tgatttttta tcaaattgat aaccatgttc
     2461 ttgcacttcc caattagaga tagcttggta taaactgtct ttaggactat catagccaaa
     2521 aaccatttgg tgtgaaaaat cttcaatagc ctccgacatt tctggaaaga taggaaaatc
     2581 catatccgca atccaaaggg gaaggatttc ttgatctttt tgaaccgcct tccattttat
     2641 ggcattactt gaaaaacgtt ctggtaaact tgtgaaatca tatgtaatca taattactcc
     2701 tgactattct acagctaaaa atatcccttg tcgccttggt gactatttta ccatttgttt
     2761 ttgatactat atactaatta acattctaaa gataaaagtg accattttaa acgatcactt
     2821 tctttattat tttaatttat cattttcata agtatggctt aattctaaat taggaaagct
     2881 ttgctgccta agggcttcat aaacaatcat acaaacagtg tttgaaacat tcaagctacg
     2941 cacatgctca tcgttcatgg gaattcgtaa ggccttttcg gcatgctcgc gcataaaagt
     3001 ttctggcaat cctttatctt cacgaccaaa cataaaataa tggtcttgat catcattgta
     3061 attttcatct gagtaaacct tgtcagcaaa tttgctaatt aggtgaactt tccctcgaca
     3121 tgacaacata aattcttcca aaccatcata gaaactaaca tctagcttat cccagtaatc
     3181 cagtcccgct cgtttcattt ttttatcatc aataggaaat cccatcggtc gtatgatgtg
     3241 caaaggagca tttgttgccg cacaagttct agcaatatta ccagtatttg ctggaatctg
     3301 tggttcaaat aaaacaatat gatttcgtcc gctatcggaa cgatgatttt tctgcgttag
     3361 tgtttcaata ttcatctcat catcactttc tagaactcat tctgactcgc aaaaaaacca
     3421 cactgctcgg agtcaatctc agcaaacaat gtggtttatg gtggtttgtt aacttaaatc
     3481 ataacaggtt tgatttaaac taacaaatcg ttgacactat tatagcactt cagtctacaa
     3541 agtcaaccta ttcatgagtt tttgggttat ttttttgata gatataatga caaatcaaac
     3601 agaataataa gcaaaaacat tggaaaatta aaaccgtaaa gaaattactt aacaaactcc
     3661 cgataaggag gatcaaaccg cctaaaattg ctagaatcgg tgaaactaag ccggtaaatt
     3721 tatttttaat atcttttttg agataaaggc tgagtactaa aacataaaga atgattaaac
     3781 tggtattatt aaacaccaca gcaatttcac taatatcact acccggcaat aagttaaatt
     3841 ttgtaaccat aaaatgaaca aaaagccaaa aaatagctac agccgtcacc gtcaaactag
     3901 ctggcaatga catttgatac ttcaaattta tgttagccat cctctcactt ttgatccatc
     3961 cgcgttccgc aaaagcttga ggtagacgca tcgtcccaag taaaagtcca ttggcaacac
     4021 ctaatacaga tagaataaca atgaaagata ataaacgccc aacactagga ccgaaaataa
     4081 tattagctgc ataattaata gcatcattac cggttgtcat aataaagcta gcacctaaaa
     4141 tttgtgtcaa accatagaaa aatgccaaat atgataataa gataagcgct ggaccaatga
     4201 caaaggcaag tggtaaattc ttcttgggat tcttgacttc tggtgcaata ttaacaaaaa
     4261 ttgtccaacc atcatatgca aaatacaatg gtaccagacc tgacacccaa ctccatccaa
     4321 catttgacgg ttgaatagct gttaaatgtt gtggaagttg tggagcttta tcagaccaaa
     4381 aagcaccaat taaagcaatt agaactaaag gaatcatttt tacaaaggtc gttaaagatt
     4441 ggaatccccc tgcaatacgc ttagcaaaga tattcatgag actaagcgct aaaatataaa
     4501 cagcagctaa aataatctgt gattccaaac ttgatgattc acctaaagta taaaatgcag
     4561 ctacccatga aacgattgct gttaaagttg gaagatacaa aaaagacgca aacaagccta
     4621 aagttgctgc taaagctggg ctaacatatt tttcgtagta agaaaaaata ccaccacttt
     4681 cactcgttct aatagctagc tcagaaattg acaaacttcc aaaaacaatc gaaaaagaac
     4741 ctagtactaa aattaccatg cctaaaaaga catctccacc agtaaattta aggatgtcat
     4801 caactttaaa ataaatgcct gaaccaatca ctacacctac aatcatagca attgtagtca
     4861 ttaatccata ggtctgttta atagaattat gattctccat aatatctcca tttctactgt
     4921 acgcttaaag gttaattata acctaattta gggcttgtca caagtttatc tgtgttattt
     4981 ttcaccagat tctgataaga gaatcatatt actcttatca gaaagatgaa aacgtttttt
     5041 aaccataatt ctcgtgcatt tttactaaaa aaaaggtatc atagagttta ttattggagg
     5101 taaaaatgag aataattgtt gtcggcggag gtaaagtcgg aaccgcactt tgtcgctcac
     5161 ttgttgctga aaagcacgat gttgttttaa ttgagaaaaa agaaaacgtc cttaaacgtg
     5221 tcactaaaca gcatgatatt atgggaattg ttgggaatgg agcaaactat aaaattttag
     5281 aacaagccga agtcaaaaat tgtgatattt ttatcgctat cactgatcga gatgaagtta
     5341 atatgatttc agcagttctt gccaaaaaaa tgggggcaaa agagactgtt gttcgcatgc
     5401 gtaatcctga gtattctaat ccttacttca aagataaaaa ctttcttggc ttctcttcag
     5461 ttgtcaaccc tgaattgtta gcagcacaat atatcgctaa taccatagaa tttccaaatg
     5521 caacttcagt tgaacacttc gccaacgggc gtgtgatgtt aatggaattt aaaattttag
     5581 aaggaaataa actctgtcat acttcaatga gtcaaattcg taaaaaattt ggtaatattg
     5641 ttatttgcgc aattgaacgc gatggcaaat tgattattcc tgatggcgat gcaactatcc
     5701 aagtcaaaga taaaatcttt gtaactggaa atcgtattga aatgatactt ttccataatt
     5761 acgttaaaaa taaagttgtc aaaaacttga tggttatcgg tgcaggacgc atcgcctact
     5821 acctactcaa tatccttaaa aataccaata cacacgtcaa actagttgaa ctcaaccaag
     5881 agcaagctga atattttagt caagaatttc ccaatgttcc tgtcgttcat ggggatggta
     5941 ctgctaaaaa tattttgtta gaagaaagcg ttacaaattt tgacgcagtt gctactttga
     6001 ctggtgttga tgaagaaaat atcattacct caatgttctt agaaagtatc ggtatcccta
     6061 aaaacattac caaagttaat cgaactagcc tactagaaat cattgacgat aaacaactat
     6121 caagcattat cacacccaag cgtattgccg tagaccatgt gatgcacttt gtacgcgggc
     6181 gtgtaaatgc tcaggattcc aacctagaag ctatgcatca tattgctaac gatcgcattg
     6241 aaaccttaca atttgaaata aaagaaacta gcaaattagc aaatcgtagc cttgcatctt
     6301 taaaattaaa gcaaaatatt ttaattgcag cgattattcg caataataaa acgattttcc
     6361 caactggtga ggatgtttta acagtgggtg atcgtatcgt tgttatcacc ttattaaaaa
     6421 atattactag aactagtgat atgttagcga ggtaggaata tgaataaaag tatgattcgt
     6481 ttccttctct caaaactttt gctaattgaa gctgctcttc tagcgattcc tctaactgtt
     6541 ggacttattt accgtgaacc gcaaagtgtc atgatgagta tagtcattac catgattatt
     6601 ttaatcatcc ttggattact gggttctctc tttaaaccta aaaattatca tatctacact
     6661 aaagaaggca tgttaattgt tgcactctgc tggatacttt ggtccttctt tggcgccctt
     6721 ccatttgtta tttcagggca aatccctaat attatcgatg ctttctttga agtatcttct
     6781 ggttttacga caacaggagc aactattctt gatgacgtca gtgtcttatc acccgcgctc
     6841 ttattttggc gtagctttac ccatcttatc ggtggaatgg gggttttagt atttgccctt
     6901 gccatcatgg aaaattctaa aaatagccat ttagaggtta tgagagctga agttcctgga
     6961 cctgtatttg gtaaagttgt ctcgaaactt aaaaaaactg cacaaatttt gtatttgtta
     7021 tatcttctta tgtttgcagt atttgcagta atattatatt ttgcaggtat gcctttcttt
     7081 gatagtatta tcatagcgat gggaactgca ggtacaggtg gttttgcagt ttacaatgat
     7141 agcattgctc attataatag tcctctgata actaacttag tttctattgg tatgcttatc
     7201 ttcggggtta actttaatct ttactatctt cttttactcc gaaaaattaa agcattcttt
     7261 ggtgatgagg aactaaaaac ctatctccgc atcgtggcaa tagcaacttt catgattgct
     7321 ctaaacgtca tcggtatgta tgataatttt cgacaaggcc tcgaacatat tttctttgaa
     7381 gtatcagcta ttattacgac aactggtttt ggcgtgactg atatcactcg ttggcctttg
     7441 ttttcacagg ttattttgct attcttgatg tttatcggtg gttctgctgg ctctacagct
     7501 gggggcttta aagtcatgcg tagcttaatc ctagcaaaga ttgcacgtaa ccaagtattg
     7561 tctacactct atcctaatag agtcatgtca ctacacatta ataaaagcgt tctagataaa
     7621 aatacacaac atggtgtttt aaaatatcta actatctatt tagctatctt catggcactc
     7681 gttcttgttt taacgcttga tactaatgat tttttggttg ttatcagtgc tgcagcctca
     7741 tgttttaaca acataggccc actcttaggt agcaatgaaa ctttctcatt ctttagtcca
     7801 ttctcaaaac tccttctttc atttgctatg atagcaggac gtttagaaat ataccctgtt
     7861 ttattgatgt ttattcctaa aacatggtca aaactataat tacaaataat aaaacggata
     7921 tatcaatctg atatatccgt tttattacgt cttaagctaa aatgatcagg gacaggatca
     7981 tttcctccgt gggctaaggg atgacatcgc aaaatacgtg caatccccat caacacacct
     8041 tttagaccat gtttttgaat agcttctatc atatacgtag agcaagttgg acgataacga
     8101 cagctagctg ggaaagctgg agaaatattt ttttggtaaa agcgaactaa gaaaatcaaa
     8161 aaagatttaa gcattaagat ttattaatag ccgcattatg caactgacta atttcttttt
     8221 tatttaatcg tcgtgcttcc ccaggacgga gacccgttaa atctaaggta ccgaattgtg
     8281 tacgagatag cttgtccact aataacccaa cttgttcaaa cattttctta acttgatgat
     8341 tacgaccttc atgaatagtt aattcaacta ctgaacgatt tttttcatga tccactttta
     8401 tgatagtata gcgagcaggt tttgttttct taccatcgat aacaacgcca cgcgttaatg
     8461 gtcgaaggtt ttctttagta gctattcctt taacacgtgc taaataaact ttatcaattt
     8521 cattacgagg atgaatcatt ttatcagtaa aatcaccatc attagtcaaa attaaaagtc
     8581 cagttgtatc ccaatccaag cgaccaacag gatatatccg ttctttgact tgtggcaaca
     8641 agtcaataac tgtcttacgt cctttatcat ctgagacact tgaaataaca ccacgtggtt
     8701 tatttaagag ataatatacc ttctcttcgt tgtaaattgg acttccttca atttcaacca
     8761 gatcaccagc ctttacttgg gtcgccaatt cattaaccac ttgaccatta atcgtgacca
     8821 ttccttgctt gattaattcc tcagcttttc tacgacttgc aatccctgca tgcgcaatat
     8881 atttattaat tctcattgtt ttccttatct tctgtattaa ttgaatccat tgaaaataac
     8941 gatacttctt cgtcaactaa ttcaattgaa gaggcgtcaa ttaagtcatc taactgatta
     9001 attcccatat aatctaagaa ataattagtt gtctcataaa ggttaggacg tcccaacact
     9061 tctttctttc cagcctcttt aattaaacca aaagctatca gtctcgttat agctccactg
     9121 gaattcacgc cacgaatatc atcaatttca atcctagtaa taggttgttt ataagcaata
     9181 atagatagaa cttccaaact tgcccgagat agactctgat taatcggagc cttcgcataa
     9241 tctctcaaca aagtcatata actatccttt gttaccaatt tataagtttg tgctgtctca
     9301 agcaataata gactagaatc gtcatcttct tcatatcgtt tagccaactt ttctagttgc
     9361 tgtattaatg ctgatggtgt caaagacaat aattctgcca tttgacgcaa actcagtcca
     9421 tcttcacctg caacaaaaag aagagcttct attgagccta aataagtcat ttctcttcct
     9481 ttcttaagat aacttgacta aaattactgt cttgttctac tgtgatttga tgtaatttta
     9541 tcaactctaa cattgccaaa aataaagtta tcatttcaga ttttgtttgg caatccgcaa
     9601 acacttcttg taatgtcgtt ttctttttaa gattaaaatg tctttcaata acgatcatca
     9661 tatcttcaat acggtagtct tctttctcaa ttaccgtttg actatttgac aattcttttt
     9721 gtttttggga catcatttga gaaaaagtca aaaaaagatc catcaccgat ttatcgtgta
     9781 gaagaattgc atcttcaaag ataacttctt gttttggttt tgaaaaatat ttggcgcgtt
     9841 cctgatgctg attagctaac tcttcactta gcactttaaa acgtctatat tcctctaatt
     9901 ggctcaacaa ttccatttct ggatcatctt caataggatt tgactccact actttaggta
     9961 ataagttacg gctttttatc aacatcagct gacttgccat aagcatatac tcaccagcta
    10021 cttctagacg catcgcctgt aatgttgcta tataagcaag gtactgctca ataacttcca
    10081 caatcggtac atcgtatata tcaacctcat atttagatac gaggtgcaag agtaagtcta
    10141 ggggcccctc gaaatctttt aacttaatat ccattatcta taatatctct ctaaagtgat
    10201 aggagttttt aagcctagaa ggcgactgag ctccgtcatc gttttgccat tttctttttg
    10261 ctttagaata aattgttctc gcagaagttg tgcagttagt tgttgctcat tcttctcatt
    10321 taagaaatct gttaattgat taaaaaacca ttgtctagaa taaggcttac cgttatgctc
    10381 aaagactaag tcaccatctt cctctaaaga ttccaaaaga aaaggaagca agtcttctgg
    10441 aagttttaaa atacgcttca tctgcgattt ttcaatactt aagatattaa aagttgtatc
    10501 aaaatcagcc ttcttcaaat tagcaatttc agccggagtt aaacctaaag ataaaatcaa
    10561 caaagcaatc aaacgaccac tcggataatc agtatcttga tagaaatttg ataaatccat
    10621 tatttgagac tttgtctgag ccaaagtgat tttttcagtc tcttgtagtc tgtaaaattc
    10681 cttcagcgtt cctctattat ataaaaagaa aagaaattga ttgactgcag ataactttcg
    10741 cttacgagct gttaacttaa attcacttag agactgttga tacaatgcca gtttttcaga
    10801 atttacatgt ccaccaatta ttttataaaa ttgctttaaa tcatagtgat aggacttacg
    10861 cgaattgaga gataatttct tactctcaat aaaattatta atatcattta tcattgtttt
    10921 taggagtgat agtatattcg tctgtaaaat catgtaggag gctattaata gcttttaaaa
    10981 tcgatttacg ggttataatg cctctaaaat cattttgatc actgataaca ggtaaaaatg
    11041 gataatctac aatcttatgc ataattgctg tcagagtagc tgcctcatta atcggctcga
    11101 tttttgtatt aaccatctca acaatatcag tctgtgccat ctcccaatct gttaattgac
    11161 ctttagattg gtagcccata atgtctgaga tactaatagt acctacatat tttttctctt
    11221 tagtgataac tggtacacgt gaaaaaccat tacttgctaa tagtaacata acgtggtctg
    11281 cattgtgtgt atccacaaaa atagcaacgt cttcagctgg aattagatag tggtccaaat
    11341 gatttagcaa aaacgactca aattctttag caatcatctt gaaaattcct ttgtcaagtt
    11401 aggatagggt tggtgttggc gagtataata ctctaccaaa accttactat ctgttatagt
    11461 tacaacagcg taaagcttct catttattgg tcctcttggc tgcaaaacac tacctggatt
    11521 gataaaaata gttttaccat ttttccaagc atctgcacga tgaagatgac catataaaca
    11581 aatatccgcg tcttcttgct gagctaataa atccaattta tcccaggtaa aattaatctg
    11641 atggagatga ccatgggttt gaacaataac agcattatct atttttgtaa ccaaaacttc
    11701 cggataacct gaatcataat cacaatttcc tgtgaccaca tgaatgcctt cccatatagg
    11761 atctgagcta ggaagttcgg aatccccatt gtgaaaaata gcatccactt ttcccaaata
    11821 atgattcttt atatctttta caatgtcacg atctccgtga gaatcactca ttacaactaa
    11881 ttttctaatt gccattttgg aaatacctcc atcaatttac ggacagcttg cccacgatgt
    11941 gatagattat ttttttcttg tgcgctcaat tcagctgctg tcctactacc ttctccgaca
    12001 ataaagagcg gatcataacc aaagccattt tcccccttag gttccgtacc aatataacct
    12061 ggccactctg cttcaactac caaactttct ttatttggag ctgacacgac taaggttgta
    12121 tgaaactgtg ctgaacgtct ttctttgtca aataccatgg ctaattcatg caataatttg
    12181 gcattattac gagcatctgt cgcgtcagga cctgaaaaac gagcagacca tacccctggc
    12241 aaaccaccta aagcatctac ttttaatccg gaatcatctg aaattaccat ttttccagtt
    12301 aattttgaaa tagtctctgc tttaagtcga gcattttctt caaaggtcat accagtctct
    12361 tcaacttctg gtaaatctgg gtaatcattt aaattttcaa ctttgagtcc taatttccca
    12421 aataattctc taaattcctt cgtttttcct tcattgtgag tcgcaattaa tatggtatcc
    12481 ccaaaattat tatcattctt taaatcaaag aatttttcta aagaagtgcc ctctttaggt
    12541 aagtgaacta agaacaactg atcatcttca ctaactaaga tggccccttt atgttgagtc
    12601 actttgatat gccttgaata ctcttgatta atcatatcaa cgaggaatgc taataattga
    12661 aaatccgaag tcatctgcac aacgtgaagc tgtagacctt caactttcaa agttgataat
    12721 agacgatgaa aaacatcttg gatagcttcg taagattcat cattaccaaa acaagtaaaa
    12781 taattaaaag cttggaaact acctaaaaac caattgcctt cagttttact ttcaaaaatt
    12841 gtttttgtca taatacgata cgctccacat ttatttcttg actaagccat tgttctgcaa
    12901 tttctttaaa accttttggg ctggcggttg tgtaaaagtg atgaccaccg tgtttatttt
    12961 gccaattatg gtttatctca aaatagttca ataaaacaga aatatcacga acggtttctg
    13021 cgccactatc aattaattta acctcagccc ccataacatt ttgaatgatg ggacgtaata
    13081 ggggataatg cgtgcaacct aaaattaaag tatctaattt accaactaat ggggacaacg
    13141 tttcataaac cacctttttg gctaaactag aagacatctg atttgattcc acaattggaa
    13201 caaatttcgg acaagcaagg gataccacag cagtatttgg agacaaagct tgaatttttt
    13261 gacgataagc atctgattta acagtcatgg gagtacctat aataccaact ttccctaaat
    13321 tagttgattt gatagctgcg ctagctcctg gtaaaataac gcctaaaaca gggatgtcta
    13381 gtttttcttt aatttcttgc caggcaactg cagttgctgt attacaagct ataacaatca
    13441 tcttaacatt tttagtcaat aagaagttaa ccatctgcca ggtaaactct ctaatctgtt
    13501 gagcaggtct aggaccatac ggagctctag cctgatctcc aatgaagatt acttcctctt
    13561 ctggaagttg acggaacatt tccttaacaa ccgttaaacc acctacacca gaatctagaa
    13621 agccaatagg cctactatcc atttttattc ctccaatatt agtgactaaa agaggctgga
    13681 caacagtcct agcctctcta tacaataacc aatcagtcaa atctacactt gaccaagttt
    13741 gcccaaatct tgtctagaaa tctgatggac tatcactcac tatttcatca ctaaacgctt
    13801 caatgctaaa acactcttat tttttagttt tgatagctgt ttttgcgtgt tttacaatat
    13861 tacggtatgt ttgttgaact ttagcttcac taggtttttg tcccatttga ctcatcattt
    13921 cacgaatagc atccggtgtt aaacgagggt gctctccaat ctctttttca atttgtttac
    13981 gagcaataaa aatgcctccc acgagaccac caaacaaagc gacgataatt aataaaatcc
    14041 aaatagttgt tgacatctat tcattctcct catttattta catgactcat tatagcaaaa
    14101 aaagaaagag tttacaaata tatgtaaaca aaatcaaagc ctctatcgtc cttgtcacgt
    14161 caatacatta ttaaattttc actagagcat ggttaagcac atagtcgagt cctttatctt
    14221 atcctaagtg ttttccccaa cttttgtgtc atccatttcc aaatgaagag agctatgtaa
    14281 aaatctacca atccatgtag caaagagcct agtcctatca aagaaaatag tgaccataaa
    14341 taagttgaac tactgttaga agtcattgtg attatataaa cagtaataaa ttcgagcaga
    14401 ccatgaatta tgttaatagc tacagcaaat aacaatgttt gccatttact catcaataaa
    14461 gagggctttg acttaatcaa gaaagccgca attaatgcaa atccgatatg tgataaagct
    14521 ctaagaacga ttacaatcgg aaaaccagct agtagaaatc caagtcctgt ccctaaagct
    14581 accaatattg caactggaac agatataaaa atacttaaaa ataaaggtac atggcttgct
    14641 aaggtgaaag aagcaggccc aataattatt ttagcaggca tgattatagg aatcatgatt
    14701 gcaaaagcta ctaaaataga agtataagaa accatttgtg ttgttttttt tgacattttt
    14761 atctcctttg aagctattat acaaaataac cttacaggtg taaagacacc tgtaaggtta
    14821 tttgtctatg tataaaattc ctgctttttt tagagcctca catatttggt caaaaactga
    14881 ctgatcttga caagaaatca tatgtgtatg tagaccatct gtcaaagagg atagcaattc
    14941 tgcattggaa acttttaatt tagcagtaaa attatcaatg tctttttggc tttgaatgtt
    15001 taagggagca gtcagcatcc cataaatagg gtgctctacc tccacattca taatgattcc
    15061 ttgataccgc aaaataattt ccagttcttc ttctgtctgt tctatgccat gctgacaaac
    15121 caatctagct gtgaattgat gagtacttag agcactggac ataaggtatc cttttggggt
    15181 agaaataata tcacattgtt gtgcacgcaa taaggcaata tctccaacaa tcacctgcct
    15241 actaacacta aaaatctttg ctaatgtgct tgccgatata gcttcttttg ttccttttaa
    15301 agtcgttaaa atattttctc gacgctcctg tgccttcata taacaaattc cttctttggt
    15361 gttatttcac aattaaaaac tggctagaat atctaaccag ttagcctcaa tcatttcgtg
    15421 caaaaagacg aagaatattt aagaatagat taataaaatc aagataaagg cttagtgcca
    15481 tcgaaactgc ccaaccatct gcgacttggc cgccttggct attgtagaca tatttaatca
    15541 tttggttatc gtaagcaatc aatccagaaa agatgatgac acatacgata ctaatgatat
    15601 aactcatacc cccactaccg ataaataaat taacaagact tgcaattaaa atcccaatta
    15661 aagcagccat taacgcttta cgcatacctg ataaatcttt tttagtctta gctccaatca
    15721 aagccattgc aaagaaaaca gccgcagatg ttataaaagc ttgaagaact gtagtttgcg
    15781 tgtaccttgc gataatgaaa cttaaagtaa acccattcaa agctgaataa atcaaaaata
    15841 atggtaatgc agctggtgtg tttcgacgcg ccgctccact agcaacaaag actaaaacca
    15901 attctaagat gattgctcca aaatacaacc ctggcatttt ttgcatgatg gcaatcatat
    15961 tttgtggaaa catatacagc ataatcgcag aaacagcagc tgacaaacct actcctatcc
    16021 ctacaagacc gtaaatctta gcaaaaaatt gattcagccc tgaatcagat tgtgtgtaaa
    16081 tgacgttatc attcatatgt attttcctat tctaaatttt aatgattatg actacctaag
    16141 aatttgcgac taaaacggat aggtttctta aaaatacgtt tgagtggacg agaagcctct
    16201 cgtactgccc aatacttatc aaccatagtc acaatataac tttctttata acgtggcggt
    16261 gcaatagtcg ctccccacat gtgcttcaaa ataatatctt cttctctagc gtttaaatca
    16321 gttaattttc gcgcattacg aaccgctatc cttggatgaa cccaggcatg gctcttgtta
    16381 aatttcgtta cacgccaatc ataataaaag aaatcatgta gtaaaccacc tcgagcagta
    16441 ctactcgcat cccaaccaaa cttcttagca attttataac ttgtataact aacatttata
    16501 gaatgctcta agcgagttga gtgatgatgt tggacaatag cttcgagttt ttggaaacgt
    16561 ggatggtcaa taaggtgacc gaccaaatcc atgaagtcct tatcgtcttg atactttttc
    16621 attataatca cccctctggg gatattataa catatgaact taaattaagc tgaaaatgac
    16681 tactcccgca gctactgcta cattaagact ttctgcttgt ccaggcattt caatatgtgc
    16741 taaaacatct gcttcttctg tcatagtttt ggaaattccc tgtccctcat ttcccatgat
    16801 taaagcaaaa taatcatatt tagggagatt cttataattt accgattgtt cagataaagt
    16861 tgtcgcaatc atagttatcc cttctgcttt acaaaagtta acaaactgct ctatttctac
    16921 cctaaatact ggtaaatgaa agtgactgcc ttgcatagaa cgaagtgttt tttggttata
    16981 caaatcagca gatttctgag ataaaaaaac agcatcaaaa ttcgctgcat ctgcagttcg
    17041 aaccattgtt cctaggtttc ctggatcctg cacatcttct aatacaagaa aacgtccttt
    17101 tttaatatct gtccatcttg tctcttgaaa ggcaatctca gctacaatcc cttgaggagt
    17161 ctgtgagtca gttaattctt tcaaaacatc atctgtaaca acaatagctc tctcgggttt
    17221 tctcaaacga tctattgccg tttctgtcac aaaaatattt aaaaattgtg caccatattt
    17281 ttcagcttcc tcaaataaat gccagccttc aataagatat gaagatttcc gatatttttt
    17341 ttgtaacaac tttttcgttt ttttaatgag attatttgat tttgaagtta taatagtagt
    17401 cataaaccta ttatatcaga aaagaggtca agttatgaaa aaagttcatt taattgtctc
    17461 agggcgcgtt caaggagtcg gatttcgcta tgcaacttat tccttagctt tagaaatagg
    17521 tgatatttac ggtcgcgttt ggaataacga tgatggaact gttgaaattt tagcacaatc
    17581 cacagattca aataaaatga cacaatttat tcagaaaatt cgaaaaggac cttcaaaatg
    17641 gtctaaagta acttatgttg atattaaact agataacttc gatgatttta atgactttaa
    17701 aatgagtaat taaaaatcat tctgcggaag tattgtttaa ttcttaaaaa aaaagtaaaa
    17761 tagataggta tattaaaaat tagaggaact aaaaacattg aaaaaaacat tgaaacgtat
    17821 cttattctct agcttatctt tgtctatgtt attattattg acaggatgtg ttagcgttga
    17881 taaggctgga aaaccctatg gagttatctg gaatactctt ggagtgccta tggccaacct
    17941 cattacttat ttcgctcaac accaagggct tggttttggt gtcgctatca ttattgtaac
    18001 cgtcatcgta cgtgtcgtta tcttaccact tggactttat cagtcttgga aagctagcta
    18061 tcaagctgaa aaaatggcgt actttaagcc tttatttgaa cctattaatg aacgactacg
    18121 caatgctaaa acacaagaag aaaaattagc agctcagaca gaattaatga cagctcaacg
    18181 tgagaatggc cttagcatgt ttggcggtat tggctgcttg ccattactca ttcagatgcc
    18241 atttttctca gctattttct ttgctgcacg ctatactcct ggagtctcta gtgcaacctt
    18301 cttgggactt aacttaggtc aaaagagtct gatacttact gtcattatcg ccattcttta
    18361 ttttgtacaa tcttggttgt caatgcaagg tgttccagat gaacaacgtc agcaaatgaa
    18421 aacaatgatg tatctgatgc caataatgat ggtattcatg tctattagtt tacctgctag
    18481 tgttgcgctt tattggttta ttggtggtat tttcagtatt atccagcagc tagttacgac
    18541 ttatgtactt aagcctaaac tacgccgaaa agtagaagaa gaatatacta aaaatcctcc
    18601 taaagcctac aaagctaata acgcacgaaa agatgttaca aattcaacta aagccactga
    18661 atctaatcaa gcaattatta catctaaaaa aacaaatcgt aacgccggaa aacaaaaaag
    18721 acggggataa aacaagcata ctaaataatg gtgtgttgtc ctaaacagtt aaagattatt
    18781 atgtaattca agtaaaaaag acttagttct atgtcctaca tagctaagtc tttttagttt
    18841 tgcctaactt ttcatataaa aagcacccta aaagtcagat aaaaatctag cttttaaagt
    18901 gcaagtaatc tagctaacag actaccttat taataaagta atcgtttttc ccaccagtct
    18961 tgaataattg cataaagcgt acaaattacc caatagataa gggctactaa gatataggcg
    19021 gtcatatagt cggagtaggt tcccccaact attttagcat tattaaagat atcaggtacc
    19081 gtaatcatcg cagtcaatga ggaacccttg accatatcta gcaaaacatt acttaaagaa
    19141 gggagtgcaa ttcttgttgc ttgcggaata ataatgcctc tataaatcgt cggtgttttt
    19201 aaccctaaag ctctagcagc ttcccactga ccatgatcaa cagctaaaag agctgctctg
    19261 ataatttctg atatatatgc actagacatc atggtaaaac ctacgataga agcagtcaca
    19321 gcatctaact gtatccctat aaaaggcaaa ccaaagtaga taagaaagag tagaaccatt
    19381 aaaggaatcc cacgcattaa ggaaatatga atattagcta gatacctcag taagcggaaa
    19441 tgagacatct tcattagagc tacaaagaat cctacgatag ctcctaaaat aaagccaacc
    19501 aaggataatc caatggtata tggtaacccc tgaataacac ttggaaaagc cttaacagct
    19561 agttccaaat taaaaatggc atcccagttt atcacttatc tactcctctc gaaatttttt
    19621 atttaacttt aattactgga attttatccg ttccagcagg ttcttttgtg agatcttgac
    19681 cagcataata tttttcagag atttttttca aagaaccgtc tttcttcata tccttgatag
    19741 ctgcatcaat ttttgttttg aggctcttat cttttttact catcacaata ccttgctcag
    19801 tcggattata cttcacatca cccattttta ctttgatatc tggatattgt tttgttacat
    19861 attttacagc aatgacttgt gtgtagtaat cattcggaat gaaatctgta cgtccagttg
    19921 atacatctcg aaggtaaacg tcattagtta cattgtcata aataacaggt tcagcacctt
    19981 gctgttttgc aattttcata tattgcgttc cagcgccacc accagctttt ttacctttcc
    20041 agtcagataa atcttttgct gtaatctttg aagaaccatc tgcacgtaca atcatacctc
    20101 caacagaata tttataaggt tgagagaaat tgtatttttt caaacgctcg ggagttgtgt
    20161 caaagttatt gacagcaaca tccaccttac cactatctac agaagtaaat gattcagcaa
    20221 ctccaacttc aacaaactta acttttatct taagtttttt agcaatcgct ttcatcatat
    20281 caatctcata accagtcaat tttttatggt catcataata acttgttgga taaagcgttg
    20341 caggggtagc aaccttaagg acaccatctt ttttaatatt gtcccatgtt gtttctttgt
    20401 tagattgacc atttgagcaa gctgctaatg tcattgcaga taaaccgact aaaccaagta
    20461 aaattgtctt tttcataagt actcctgtag attaaattat attaaaatgg tttggcgtac
    20521 tctatcgaaa attaaattta atgaaaatta atacataaat ttacgataat aatacaacca
    20581 ttgttaagag taccatattg tatccgtttt cgcaaaaata ttgtttactt ttcaaattaa
    20641 tggtaaacag gccctttgaa ttgcttctct ttttcaaaca actctgcaac agcaagcaat
    20701 aaatcttctc tccctttagc cgctgtcaat tgtacaccta aaggtagccc ctctttagtt
    20761 agatgagttg gtatggctaa agaaggttgc cctgtcaagt ttggctgatg tgtaaaaggt
    20821 gtccaggcca ttgagtcttc aaacattttc caaattaaat cctgttgttc acttactgag
    20881 aattctccca tatgcctcaa ttgtttctgg agtgtctcgt ctaaatcaaa ttgtccatga
    20941 aaaggagctg gttgattagt tgctgctgtt aaaattaaat cataattttc atgaaacctc
    21001 gccatggtag ctgcaaattg atcccatgaa tctaagagtt tactgtaatc tttagccaat
    21061 accctttgtc cactctgata catagcccag gtcattaatt ccatatcgct aaattccatt
    21121 gaacgcccta aactcttttc gatgtcatca aacattgctg ctgtttcaac gctattcatc
    21181 atacaataag ttttcataga atgaatacca tctaaaggtt gttctactaa ttctaaatga
    21241 ttcccttttt gagacagaaa atctgcggct tctttaatag ctgctttagc ctcacttgat
    21301 acctttgttt ttaagggaga atccatcaat actgctattt ttaggggttt actaacgcta
    21361 aactcgaaaa gactctcttt gctgagtttt ttcaggggaa atgggctttc tacttgatat
    21421 gattgtaaat aatataatag tcgttttgta tctctaactg actttgttaa agcaaaatga
    21481 ctagaagccc cttgccaacc tctataggat gaaggtccaa ctggtatacg tcctcgactc
    21541 ggttttaaac caatcaaacc attaaaactt gctggaattc tgattgaccc tcctccatca
    21601 ctagctcctg caattggaac cattcctgaa ctaacagctg cggctgcacc accactagag
    21661 cctccagcat ttcttgaatg gtcaaatgga agattaacat tcccgtgaag ttgaccatca
    21721 ctaatattct taaaaccaaa ttcaggggta tttgttcttc ctaaaataat aaaccctaat
    21781 ttttcaaagg actgcactaa atagtccgtt tgtttagcat gatagtgttt aaataattgt
    21841 gaacctgagg ttgagagttg tcctttttga ttctgtccca aatctttcag aagaatagga
    21901 actcctgcaa aaggagcatt tgattcattt gcgtatttgg ctgcttgacg cgcctcatta
    21961 tattgcttgc tgacaacagc attaacgctt acattctgtt cctcgatctt gtagattgct
    22021 tgctctacta attcttgcga cgaaatctta tgctgtttta tcgcttgtac catagcggta
    22081 gcatctttaa aaaccatata aacacctcat aaaatgtatt tttattataa gataacaaaa
    22141 aatacttaaa aaaaatagaa taacgtctag actctagata ttattctatg atttatacta
    22201 ttaatttgtt ttttcaacac taatgatttc aacttgatag ctaccagcag gtgattcaat
    22261 tgtagctaag tctcctgttt ttttaccaat taaagcatga gcaattgggc tttcattcga
    22321 aatttttcct gaaaaaatgt cagctccagc agcaccaacg atatggtaag tatctttatc
    22381 atttgtacca acttcttgga caaggacagt tttaccaatt gcaacctcat ctttagcaac
    22441 tgcatcacta tcaataattt cagcataacg aatttttgtt tctaaaattt gaatttgacc
    22501 ctcaacaaat gcttgttcgt cttttgctgc atcatattcg ctattttctg ataagtcacc
    22561 ataagaacga gcaatcttga tacgctctac aacttctggt ctacgaacta attttaactc
    22621 ttcaagttct ttttcaagtt gatctttttc cacttgtgtc attgggtaag ttttttctgc
    22681 cattttattt ctcttttctt ttcatttttt caagtcaaag atataaacat gatgactcgc
    22741 accatgttta taagcttagt taatttgact attgatatat ttttcaacgt tagccttgtg
    22801 tgtttcaaaa tctttagcgt agtatacatt tccggtcttc acatcagcga caaaataaag
    22861 gtaatctgtt gaagcaggtt ttatagtcgc ttcaattgct gagatacttg gactatcaac
    22921 tggtccaggc atcaaaccag tattagtata gatgttgtat ggtgatttaa ttgaagtatt
    22981 aatttgagca tcttcagcta aacttgtttt atcacctaat ttccccatag catacaaaat
    23041 tgcaatgtta ctttgaagag cttgaccggc tgataatcgg ttataaaaga cacttgctat
    23101 tttacggcga tcttcgtctg ttgatccctc tttttcaaca agagatgata atgtcaaaac
    23161 atcatttaca gacatattct tcgctttaat agtgttatag taaggtgcta tcttagtatt
    23221 cattgttgaa atcatatctt caacaagtcc ttctaaggtt gtatctttat aatagttgta
    23281 ggtagctggg aataaatatc cttccaattg ataaatagct tttgatttat caggtaaact
    23341 tcctagtaaa tttggatatt tagctaccat ttttttaata aacgtttcat cttgaacaag
    23401 tttcaagaaa tcatcagctt tataaggtgt acttgtagtc tttgtatcaa ttttattaga
    23461 ctcaatagct ttagcaatct gtttaatagt gt
//
LOCUS       DATRNX010000023        23304 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRNX010000023 DATRNX010000000
VERSION     DATRNX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23304
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            149..1483
                     /locus_tag="VBC81_000719"
     CDS             149..1483
                     /locus_tag="VBC81_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6911777.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1551..2339
                     /gene="pflA"
                     /locus_tag="VBC81_000720"
     CDS             1551..2339
                     /gene="pflA"
                     /locus_tag="VBC81_000720"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6911778.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2456..3391
                     /locus_tag="VBC81_000721"
     CDS             2456..3391
                     /locus_tag="VBC81_000721"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6911779.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3451..4104
                     /locus_tag="VBC81_000722"
     CDS             3451..4104
                     /locus_tag="VBC81_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6911780.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4134..5135)
                     /locus_tag="VBC81_000723"
     CDS             complement(4134..5135)
                     /locus_tag="VBC81_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6911781.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5132..6157)
                     /locus_tag="VBC81_000724"
     CDS             complement(5132..6157)
                     /locus_tag="VBC81_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6911782.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6173..7105)
                     /locus_tag="VBC81_000725"
     CDS             complement(6173..7105)
                     /locus_tag="VBC81_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6911783.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7129..7923)
                     /locus_tag="VBC81_000726"
     CDS             complement(7129..7923)
                     /locus_tag="VBC81_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911784.1"
                     /translation="MSHIKSENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(8082..9533)
                     /locus_tag="VBC81_000727"
     CDS             complement(8082..9533)
                     /locus_tag="VBC81_000727"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6911785.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            9657..11291
                     /locus_tag="VBC81_000728"
     CDS             9657..11291
                     /locus_tag="VBC81_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6911786.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            11426..12646
                     /gene="pepT"
                     /locus_tag="VBC81_000729"
     CDS             11426..12646
                     /gene="pepT"
                     /locus_tag="VBC81_000729"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6911787.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            12675..13166
                     /locus_tag="VBC81_000730"
     CDS             12675..13166
                     /locus_tag="VBC81_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6911788.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(13153..13350)
                     /locus_tag="VBC81_000731"
     CDS             complement(13153..13350)
                     /locus_tag="VBC81_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6911789.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            13396..13920
                     /locus_tag="VBC81_000732"
     CDS             13396..13920
                     /locus_tag="VBC81_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6911790.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            13931..14614
                     /locus_tag="VBC81_000733"
     CDS             13931..14614
                     /locus_tag="VBC81_000733"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6911791.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    14635..14760
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14775..15305
                     /gene="infC"
                     /locus_tag="VBC81_000734"
     CDS             14775..15305
                     /gene="infC"
                     /locus_tag="VBC81_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6911792.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            15345..15545
                     /gene="rpmI"
                     /locus_tag="VBC81_000735"
     CDS             15345..15545
                     /gene="rpmI"
                     /locus_tag="VBC81_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6911793.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            15603..15962
                     /gene="rplT"
                     /locus_tag="VBC81_000736"
     CDS             15603..15962
                     /gene="rplT"
                     /locus_tag="VBC81_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6911794.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(16205..18349)
                     /locus_tag="VBC81_000737"
     CDS             complement(16205..18349)
                     /locus_tag="VBC81_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6911795.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            18484..19641
                     /locus_tag="VBC81_000738"
     CDS             18484..19641
                     /locus_tag="VBC81_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6911796.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            19641..20318
                     /locus_tag="VBC81_000739"
     CDS             19641..20318
                     /locus_tag="VBC81_000739"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6911797.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            20412..21479
                     /locus_tag="VBC81_000740"
     CDS             20412..21479
                     /locus_tag="VBC81_000740"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6911798.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            21480..22646
                     /gene="aroC"
                     /locus_tag="VBC81_000741"
     CDS             21480..22646
                     /gene="aroC"
                     /locus_tag="VBC81_000741"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6911799.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            22730..23065
                     /locus_tag="VBC81_000742"
     CDS             22730..23065
                     /locus_tag="VBC81_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6911800.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         7622 a         3661 c         4464 g         7557 t
ORIGIN      
        1 tgctataaaa tgccataaac aatattatcg actatgcgct cagtgctatt atatgatata
       61 ataatagcat tatctaatag aagaacagtt ggtagagtta atcgctatca gctaaattca
      121 aataattttt atttaggagt aattttccat ggaagaccct ggcagtcaga gtttgttact
      181 acaatttgtt attttattaa tattaaccct gtttaacgcc ttcttttcag catctgaaat
      241 ggctttagta tcgctaaacc gctctaaagt agaacagaaa gctgaagaag gggacaaaag
      301 gtatcgacgt cttttggacg ttcttgaaaa tcccaataat tttttatcaa caattcaagt
      361 tggtattact tttattagcc ttttgcaagg ggctagttta tctgcctcac taggacatgt
      421 gatttcagga tggctaggaa attcagcaac agcccgtaca gcagggagta ttattgcgct
      481 aatcttttta acatatgtct ctattgttct aggagaatta tatcctaagc gtattgctat
      541 gaatttgaaa gatcgtctag cgattgtttc agctcccatt attatctttc tggggaaaat
      601 agtaagcccc tttgtatggt tgctctcagc ttcaactaac ttattgagta gaatcactcc
      661 gatgactttt gatgatgctg acgagaagat gacacgtgat gaaattgaat atatgttgac
      721 gaatagtgaa gaaactttgg atgctgaaga aattgagatg ctgcaaggga ttttctcgct
      781 agatgaaatg atggcgcgtg aagttatggt tccgcgtact gatgctttca tgattgacat
      841 caacaatgat gcacaatcaa atattgaagg aattttatca caaaactttt ctcgtgtccc
      901 tgtttatgat gacgataaag atagagttgt tggtgtttta cataccaagc gcttattgga
      961 agcgggcttt aaaactggtt ttgatactat cgacttgcga aaaatacttc aggaacctct
     1021 ttttgttcca gaaacgattt ttgtagatga tcttttaaaa gcattacgca acactcaaaa
     1081 tcaaatggcc attttactag atgaatatgg tggcgttgct ggtttagtaa ctctagagga
     1141 tttattggaa gaaattgtcg gcgaaattga tgatgaaaca gatacagctg aacaatttgt
     1201 cagggaaatc gatgagaata tctatattgt tctaggaact atgacgctta atgaatttaa
     1261 tgattatttt gagacagagt tagaaagtga tgatgttgat acaattgcgg gttattattt
     1321 aactggtgtg ggtagtattc caaaccaaga ggaaaaagta gcttacgaag tagacagcaa
     1381 agataaacgc atcactttga ttaatgataa agttaaagat ggtcgtataa caaaattgaa
     1441 agtgctattg tctgatatag aacagaatat tgaaaaagac taagattact tagtcttttt
     1501 ttcatgaaaa tgatataata aagtcataaa aacggttaca aggtgatgaa atggcagaaa
     1561 tagactataa aaaagtaaca ggaatgatcc attctacaga aagtttcggc tctgtggatg
     1621 ggccaggtat tcgttttatc atttttatgc aaggatgtaa aatgcgttgt caatactgtc
     1681 ataatcctga tacttgggaa atggaaacta acaattcaaa ggaacgcacc gttgaggatg
     1741 ttctaaaaga agctcttcgt tataaacatt tttggggaaa agatggaggt atcacagttt
     1801 caggtggtga agcaatgctg cgaattgact ttattacagc tctctttatt gaggctaaga
     1861 aactaggtat tcatacgacg ctagatactt gtggttttgc ttatcgagca acaccagaat
     1921 accatgctat ccttgaaaaa ttgttagatg ttactgattt ggtactcctt gacttaaaag
     1981 aaattgatcc agaacaacat aaaattgtaa ctcgtcagtc taacaaaaat attttacagt
     2041 ttgctagata tttatctgat agaggtactc cggtatggat tcgtcatgtt ttagttcctg
     2101 gattgactga tattgatgac catttaaaac gtttgggtga atttgtccaa acacttgata
     2161 atgtcgataa atttgaagta ttgccttatc atactatggg agagttcaaa tggcgtgaat
     2221 taggaattcc gtatccatta gctggtgtga aaccaccaac tccagaacgt gttaaaaacg
     2281 ctaaagatat catgaaaaca gaatcctata cagaatatct aaaaagaatt caaaattagt
     2341 tttaagttga attttattca acttttttag ttgcaaaggg cttcctagta agattttttg
     2401 ctaagttatt caatttttgc tacaataagg tgaatataaa agaagaggta aaaacatgtc
     2461 taaaatttta gtctttggtc accaaaatcc agattcagat gctattggat catcagttgc
     2521 ttttgcatat ctagctaaag aagcttgggg gcttgacaca gaggccgttg ctttaggaac
     2581 tccaaatgaa gaaacagcat atgttttaga ttattttggt gttcaagcac ctcgagtagt
     2641 agaatctgca aaagcagagg gtgtagagac agtcatttta actgaccata acgaattcca
     2701 acaatctatt tctgatatta aagatgtgac agtttatggt gttgttgatc atcatcgtgt
     2761 tgctaacttt gagactgcca atccgttata tatgcgtttg gaaccagttg gctcagcatc
     2821 atcgattgtt taccgtatgt ttaaggaaaa tggagtatca gtacccaaag agttggctgg
     2881 tttattatta tcaggtttga tttcagatac tcttcttttg aaatcaccaa caactcatgc
     2941 ttcagatatt ccagttgcaa aagaactagc agagcttgca ggtgtaaact tagaagaata
     3001 tggtttagaa atgcttaaag caggtactaa cctctcaagt aagactgcag cggagttaat
     3061 tgatattgat gctaaaacat ttgaattaaa tggagaagct gttcgtgttg cacaagttaa
     3121 tactgtggac attaacgata ttttagcacg tcaagaagag attgaagttg ctattcaaga
     3181 agcaattgtc acagaaggtt attcggattt tgtattgatg attactgaca ttgttaattc
     3241 aaattcagag attttagctc tcggttcaaa tatggctaaa gttgaagcag cttttgaatt
     3301 tacacttgaa aataaccacg cttttcttgc tggggcagta tcacgtaaaa aacaagttgt
     3361 tcctcaattg acagaaagct acaatgctta attaatcatg taaaacgggc tgcgtcccgt
     3421 ttttttagtc atacatttag gagcgttttt atgatattaa cgtttaatcc aggaaagtta
     3481 gaaagacaag agttttttaa ggagcttatt aactatcttt ggatacatga tgatgtaaca
     3541 ctaagacaaa taaagtccca ctttacagac tattctaaaa ttgaccgttt gttagaagaa
     3601 tatattaatc atggatatat ccttcgccaa aataaacgct acagccttaa tcttcctttt
     3661 ctgagttcgc tggatggatt agcattagat gatttggttt tcatagattc tgctagtcaa
     3721 atctatcagt tattacagaa gcgaaaattt gtgacaaatt tagataacca aaccaatcac
     3781 ttggtatttg tagaagagac agattttgaa agaaacactt tgactctttc taattatttc
     3841 tataaactaa ctaactgtta tccactttca agagaacaaa aaaagcttta tcaactttta
     3901 ggagatgtta attcggaata tgctttaaaa tatatgtcaa gctttatatt gaaatttctt
     3961 cgcaaggata gtgttaagca aaaacgtaca gatattttta tccaagcttt agaattactt
     4021 ggatatatta gcttaaacca agatactacc tatcgtttaa atgctaaact tgatgttgaa
     4081 gctttaaaga tttacttaac ataaaaaaag actggaagtt tgtccagtct tttttagtca
     4141 acttttgtca tcaaccagag aaaatagggg gcaccaatta ttgatacaac caatccagtg
     4201 ggtatacctg taccaactaa gaggagacga cctatagtat ctgataccaa taggataata
     4261 gcaccaataa gaagacagct aggtagtata attttatggt tatttcctaa tagtcgtcta
     4321 cttaagtgcc cagcgagtaa gcctataaaa gtaatattac caattaagac gacgctaata
     4381 gcagcaaggc tacttgccag catcaaagta tatattcttt ccttttttaa atgaagccct
     4441 agagcgagag cagtctgctc gtttaggttc ataatattaa ggtggcgact gcgactatat
     4501 gctaatcccc ataacaaaat gagaatcggg ataaaagtga taatggtagt ccaggaacta
     4561 attgtaattt ttccactaag ccattcaacg atcatatcca ttttttgatt attagactga
     4621 ctaataatag aaatcataac gcctgaaatc attgtagata ttccgatacc agtgatgatt
     4681 aatcgtgtag ggctaatatt gtgatttctt ctgtatgaca taagatagac aagaaaaata
     4741 gttaccaacc ctccaaacat agcaaatagg ggtaaaaagt ataatatagt tggattgcta
     4801 acattaaatg ttcctatggt tagagcgatt actaatccag ctccagcatt gataccaaga
     4861 ataccggaat cagctagtgg atttttggtt aatgtctgta atagcaaccc agcaattcct
     4921 aaagaagctc ccccaaagat acagacaata atacgaggta aacgaatgtt agtaatgata
     4981 aaaaggaagg catcatcaga ttttccactt attagtttta aaacgtcaat aacggaagta
     5041 ttagcatagc caagtgaaat tgatactagt attaataata gtaggataat gacagatgat
     5101 attaaaacaa aaggagcttt atttttttga atcatagatt tttccctttt ctaattaacc
     5161 ataggaaaca tggaagccct acgatactga ttatagaact aattggtgtc tctgaagggg
     5221 gatttataat acgagatgat aagtctaccc agatcatgaa agtagcccct gaaaatccta
     5281 ttaaaggtaa taacagacga taatctttgg gaataaaaag cttaataaaa tgtggaataa
     5341 tcaatccaat gaaagaaacg gttcctatca acgcgactga tgaagccgat aaaaacaaaa
     5401 caataagtaa tagaaacgca gtcattaatt gagttttttg tcccaatgct ttggccacag
     5461 attcattaag gctcaagata gttaattgat gtgctaggag ttgtgaaata agtaaaccta
     5521 aaataatgat aggtgctatt atgattagca ttttccagtt aatttgggat aaaccacctg
     5581 cttgccaacc gataacagtt cgattgagtt taaagtataa tgtaaccact tgtccaacag
     5641 acgtaaaaag tgtggagatc atcgctccag ctaaaatgag tcgtagttgg tggtaaccct
     5701 tttgctttgt ataagagagt gtgaagacta aaagacaaga aatgacagac cctagtaagc
     5761 agacaatgag aatcgtacta tagtgcatgc tccctaaaaa agcatatgca acaacaaggg
     5821 ctaacccagc tccggcgtta atacctagta gtccaggatc agcaatggcg tttcgagtga
     5881 ctccctgcat aatagcacct gcttgtgaca tagctgcacc tactaagata gctgcaatta
     5941 tgcgtggtag tctaatgtca aaaataacat cctgagcatt agagtttttt aagggatttt
     6001 ttaaaacagt tattaaatca ctagtcttga aattgatagc accaaatcgt aagctggcgt
     6061 aacaaccaat aagaaatagt ataagtgtta tgaaaaaaac aagccaaaaa tgatttgact
     6121 tatttttttg aacgagtttt tttgaagctt taaacataag aggagctttc ttttaatttg
     6181 tattttcttt gatagccttt gtaaatgatt ttaattgagc ttctaaagat agagggtcag
     6241 agaaataaaa cacgtcgtag ttactttcta tgatgtgccc ttttttgaca gctggtaaat
     6301 tcttccagac atcactttct ttaagtgatg aagctgcttt tttagtcgtt ttgtttatat
     6361 taacaagggc ataatctcca acgtaatcac cgattgcttc ttgcgaaacg gtaaaccacc
     6421 cttttttaaa gacatctttt ttgacttttt ctggggcagc ataacctagt gaatcataga
     6481 ttagttctcc accgcgtcca aaattattac catataaata gatattttta tcataaaaat
     6541 ccataatagt aaaagtagtg ttaggcttta agatatggtg taaatctttt ttggcagcga
     6601 gagttttagt tttccattgg ctaacccact gattagcttc tttttcttta ccgaatactt
     6661 tccccaaggc tggcatcata tctaaataat tttgtgcacc atatttaata actaaagttg
     6721 gtgcaatttt tttcagagta ttgatgtttg gatcttgatc gaaaaccatg attaaatcag
     6781 gtttttgtgc ggcaatagct tctgtatcat cagcagttaa ttttttagct tctttcagtt
     6841 gcttaccaaa aacggggcta tctttttcta agtctaaact gtaacttgaa acattaacac
     6901 ctagttttaa taaataccca gtgtaagaat atgtaaaatt aattactttt ttcggatttt
     6961 caggaatttt tccataatag gtgaagcctt caattttagg cattttagaa atagttgttt
     7021 tagtgctttc ttctttagtt tgttgtctgc aagataccaa aaagaaggtc agtagtgtga
     7081 ggacaataat tccaattttt ttcataagat aacatctcct aaataatatt aattttgatc
     7141 agttgataat tgataagata acaaaacagg ttgtttacta atagggtcct gaatgatatg
     7201 agcatcaatc ttaaaaatat ctttgataat atctttagtc atgatctgac taggactacc
     7261 ttcatagata attttaccgg ttttcattgc aactagataa tcagaatagc gcgcagacag
     7321 gtttaggtcg tgtaggacca taataattgt tttttgcgtt tcgttattaa gttttttgag
     7381 caattctaag acttctaatt gatgattcat gtcaagataa gttgtaggtt cgtctaaaaa
     7441 gatagtatct gtatcttgtg ctaaagccat agcaatccat actttttgcc tctgacctcc
     7501 tgaaaggtca tcaacatctc tattagcaaa ttgcgctacg ttagtcattt ccatcgccca
     7561 atggatttta ctacgatcat catttgtcaa attgccaagg tatttttggt gtgggaaccg
     7621 tccataagat attagttcat aaactgttat cccttcagtt gcttctaaca cctgtgggag
     7681 aagtgcaatc ttcttagcaa tttcctttgt gggtaaatgt gcaatatcat gaccatcaat
     7741 agtgatagta ccatctttta ttttatggat ccgtgtgaga gccttcaaaa gagtagactt
     7801 tccacatcca ttggcaccaa taatcgttgt tattttttgg ttaagtatac tgagggaaag
     7861 attattgatg atttctttct gatcgtaaga tacaataatg ttttctgatt ttatgtgaga
     7921 catagagaac ctttctagca aaaaattaag ttcattataa catagaaaaa aggcttacaa
     7981 aagccttttt tagcattaat taagaaaaat gtgaacgaaa tgataattat ttagaatgat
     8041 tcaaaatagt atcaataatc attattgttt agcttttttt atcatttaag gtattttttc
     8101 gcaacttcta aatctccaga gtaattgtca cgatttcctt tgataatttg ataagcatct
     8161 gcccctttac cagcgataat aattgcatct agtttgcaat tagtaagagt actggcatta
     8221 gcaattgctt cttctcgatc aatgatgata gtaaccggac gattaatatg gcttgcgatt
     8281 tcttgtgaga taactaaggg atcttcaaaa ttaggatcat ctgcagtgag tatcacttgt
     8341 agattaggat gttgattaat gacatctccg aagtctttac gacggctttg acctttatta
     8401 ccaggagctc cgagcactaa tataatatcc cctttttggt gttcctcaac aacagctaat
     8461 aatttcttca aactgtctcc gttgtgggca tagtcaacga aaatctttgc gccgttggtt
     8521 tgtgttagga cttccatgcg gccagggacg gtagtttgtg caattccgtt ttttatatct
     8581 tcaattgaaa caccaagtcg gagacaagca agtccagctg cgagagcatt ttcttgattg
     8641 aattttccaa tcaggttgat attgtaggtg ttctccaact ttcctttgac atggaaagaa
     8701 aaggcctgtg attctgtgat aacattttca gaataatctc catagaaatc atgaggaata
     8761 tattctacct gttctttgac aatattaaaa tgatccatct gactatttac aacgacagca
     8821 ttgctattct ccatcaagag ccgtttgtgg aaaaaataat cttcgaatgt agggtgctcg
     8881 ataggaccaa tatgatcggg actaatattt aagaaaacgc caacgtcaaa ggttaaacca
     8941 taaacacgtt tggttaggta tgcttggctt gatacttcca tgactaagtg tgtcatttga
     9001 ttctcaacgg ctgtagccat catccgaaat aaatcaagac tctcgggggt tgtgagatgt
     9061 gatttaaaga aagatttacc atctaatgtt gtgttcatag ttgagagcat agcaggacga
     9121 tggtttactt ttagcatatg ataagcaaag taagccgcag ttgtttttcc ttttgttcca
     9181 gtaaaagcta gaagtttaag tttgttttgt gggttattat agaaagacat agatatcaaa
     9241 ctcatggctt ttttgatatc attgactaat atcactggaa tatcggcgcc gtaatctatc
     9301 tcagaaacgt aaaaagaaag gcctgctgtg attgcagaat ctagatattc ttttttaaaa
     9361 gtggcaccct tagcaaaaaa taaagtatca gatgaaattt gacgactatc atatgataag
     9421 gcattaaaag ttacattttg tgtccagtta taataataat gctcatgaaa caaaatctct
     9481 ctgaaattat ggtcattttt taaaatttct aaaattttat ctattgtaat catactcctt
     9541 attttagact ttatgttctc gttttacaag tctaaggtaa aagtttataa taaagtgata
     9601 tttctgagat ttttgtcttt taaatgctaa ataatattga aaatagaaag tttaccatgt
     9661 cacaaaaaac aacaaaagtt agtcagcaag aacaaatggt caaagggacg gcttggttaa
     9721 cagcagggaa tttcattagt cgcttgctgg gagccatcta tattatccct tggtatgttt
     9781 ggatgggaaa gcatgctgcc gaagcaaatg ctttatttgg gatgggatat gaaatctatg
     9841 ctttatttct tttgatatca actgttggaa ttccagtagc agttgcaaaa caagtatcta
     9901 agtataacac tttaggtaaa gaagagatga gcatttatct ggttcggaaa atcttgcagt
     9961 ttatgctgat tttaggtgga gtttttgcat taattatgta tattggttca cctctatttg
    10021 cttcacttag taaaggaggg caagaactcg ttccaatatt gcgtagtcta actctagctg
    10081 ttttagtttt tccttcaatg agtgttttac gtggtttttt tcaaggtttt aataatttaa
    10141 aaccgtatgc cattagtcaa gtcgctgagc agattatacg tgtcatatgg atgttattaa
    10201 ctgctttcta tattatgcgt ctaggatcag gagattatat tgctgctgtg acacagtcaa
    10261 cattcgccgc ctttgtcggt atgtttgcta gtatagctgt gctgctttat ttcttatggc
    10321 gttataatat gctatctgcc ttgattagta aaactccaga gcacattaaa ttagatacta
    10381 aagaaatcct aattgagact gttaaggaag caatcccttt tattattact ggtgcagcaa
    10441 ttcaaatttt caaattgatt gatcagtttt catttgggaa tacgatggcg ctctttacga
    10501 attattcaag tgaagaatta cgggttatgt ttgcttactt ttcaagcaac cctggtaaag
    10561 ttacgatgat tctaattgct gttgctaccg caattgctgg agttggtatt ccattgttaa
    10621 cagagaattt tgttaagaat gataaaaaag ccgccgctcg tttggttgtt aataatttac
    10681 aaatgttact aatgttttta ctgccagctg ttgcaggatc cgttatttta gcaaaacctt
    10741 tgtatacagt cttttatggg ttaccacagg gacaagcttt agggcttttt gtcatatctc
    10801 ttattcaaac aattatttta tctatttata ctgttttagc accaatgcta caagcattgt
    10861 ttgaaaatcg taaagctatt atttacttcc tatatggtct ggtggcaaaa gttattttac
    10921 aagttccctc cattttcctt ttccatgcct atggaccact tttctcaacg acagtagctc
    10981 tttgtatccc agtaattctt atgtatttga aaatacatga aattacagga tttaaacgcc
    11041 aagctattcg tcgtacaagt gctttagtcc ttattctaac gctattgatg agctttatta
    11101 tttctatgat aatatggtta atgaacctgg ttattgtacc cgatagtaga ctggtaagtc
    11161 tagtttacat tatcgttatc ggtgcaatag gtttaggggt atatggcttt atggcactgg
    11221 ccacccatct acttgataag atgattggta gtcgtgctca agacttacgc cgaaagttgc
    11281 accttaacta atgaaaaaat aggttagaca gaattgttta acctagtttt ttgacgacca
    11341 aaattatttg tgaaatttta aggtagtcac aatatagtga gcttattttt atgttataat
    11401 taaatggaga aatatgaggt gaaagatgtc ttacgaaaag cttttagaac gatttttaac
    11461 atacgtcaaa ataaatacaa gaagtaatcc taatagtaca caaacgccaa caactcaaag
    11521 tcaagttgac tttgctttaa cagttttaaa accagaaatg gaagcaattg gtttaaaaga
    11581 tgttcattat ttaccttcta atgggtattt ggttggaacc ttacctgcta caagcgaccg
    11641 cttacgccat aaaataggtt ttatatccca tatggataca gctgatttca atgctgaaaa
    11701 tattactcca caaattgttg actataaagg tggagatatt gaacttggag actcaggtta
    11761 cattttaagt ccgaaagatt ttccaaattt aaataattac catgggcaaa cattgattac
    11821 aacagatggt aaaaccttac tgggagcaga cgataagtct ggtatagcag aaatcatgac
    11881 agctatggaa tatttggctt cgcatccaga aattgagcac tgtgaaatta gagttggctt
    11941 tggaccagat gaagaaattg gtataggtgc agataaattt gatgttaaag attttgatgt
    12001 tgattttgcc tatacagtgg atggtggacc actaggagaa ttacagtatg aaacctttag
    12061 tgcagctggt ttggagctta catttgaagg acgaaacgtt caccctggaa ctgcaaaaaa
    12121 tcaaatgatt aatgctttac agcttgctat agattttcat agtcaattac cagaaaatga
    12181 acgtcctgaa caaacagatg gctatcaagg attttatcac ttatatgatt taagtggaac
    12241 agttgatcaa gctaaaagtt catatatcat tcgagatttt gaggaagtta atttcttaaa
    12301 acgtaagcaa ttggctcaag ctatcgctga taatatgaat gaagcattac aatctgaacg
    12361 tgtaaaggtt aaactatacg atcaatatta caacatgaag aaagttattg aaaaagacat
    12421 gacacctatc aacattgcta aagaagtaat ggaagagtta gacatcaagc cgatcataga
    12481 accgattcgt ggtggtacag atggctctaa aatttccttt atgggaatcc ctactcctaa
    12541 tctttttgca ggtggtgaaa acatgcatgg acgctttgaa ttcgttagtc tacaaacaat
    12601 ggaaaaagca gttgatgtta ttttaggcat cgttgctaag gattagtaaa gaaatatcag
    12661 attggagtgg ttaaatgata aaattatttg ggaagatacg ttatcactgg caacctgaac
    12721 tttcttgggc tattatttat tggtcaatag ccattgcacc aatttttatt ggtttatctc
    12781 tgctatatga aagaactgag attccgagtc aggtatttgt tctatttgcc atctttattg
    12841 ttttagtagg cattggattt catcgctatt ttgttattga agaagatgga tatcttagga
    12901 ttgtatcttt taacttttta agacgatcta agtttccaat tgaagatatt gctaaaattg
    12961 aggtcactaa atcaagtgtc actatcaaat ttaataataa ccatgagcgt attttttaca
    13021 tgcgaaaatg gcctaaaaaa tattttttag atgcactagc tattgaacct aaatttaagg
    13081 gagaagtgga gcttctcgat aatttgatta aaatggatta ttttgaatgt tatcgctacg
    13141 ataaaaaagc tcttacaaaa tcgtaagagc tttggtggga catgatttaa ctgctaaaac
    13201 agtatcttga tcagaacttg gaatttcttt ttctaggtta tctgtatcgg aaaatttaac
    13261 gataccatca tcttgataat caaaaatatt tgaatatgtt tggcaaagcc cgcaggcaat
    13321 acatttttct ggaattagtg caactttcat agtattattg taatacttat tgatacgatt
    13381 aacaaggggg aagtcatggc gaaaaaacca tgggaaaaaa aagttgtgga aaacaatagt
    13441 catagaaaag acaaaattac aagaacgagt agaggtgtgg tgtcaagtac tccttggatt
    13501 accgctttct tgagtgcatt ctttgttatt gtcgttgcta ttttatttat agtattttat
    13561 acttcaaata gaggagaaga cagagcaaaa gaaacttctg gtttttatgg tgcttcaagt
    13621 caaaaagtta atagttctaa aacgaaaaaa gcatctacaa gtaagaaaac aacgtcatca
    13681 tcaaaaacta aaactagtga taaatcaaca acatcttcta gtacagtagc aagttcgagt
    13741 gagacatcga catcatcttc agtaaacggt ggaacaatcg tagttcaacc aggtgaggga
    13801 gcagcctcca tagctgctcg tgcaggtatc tcagttgctc gtttacaaga acttaatcct
    13861 ggtcatatga cctcagggta ttggtatgct aatccaggcg atcctgtggt tgtaaattaa
    13921 ggggaggatt atgaactcaa ttaatattgc tattgatgga ccagcatcaa gtgggaaaag
    13981 tactgtcgct aagattattg ctaaaaattt gaattacact taccttgata caggtgctat
    14041 gtatcgttgt gctacttatc tggctttaca gcatggatat gaagcacaag atgtttcgaa
    14101 aattttagga ctcctagctg aaagaccgat ttcttttggt aaagcagaag atggcagcca
    14161 gacagtcttt cttggcacag aagaagttac tttagctatc cgtcaaaacg atgttactaa
    14221 caatgtctcg tgggtttctg ctatcccaga gattcgagaa gagttagtaa accagcagcg
    14281 tcgtatagcc aaagatggtg ctattataat ggatggtcgt gacataggaa cagttgtttt
    14341 gccagatgca gaattgaaga tttttttagt ggcttctgtt gatgaaagag ctgagcgtcg
    14401 ctttaaagaa aatcaagaaa aaggcattga gagtgatttt gaaacactga aatcagaaat
    14461 tgctgctcgt gattataaag atagccatcg agaagtttca ccattaaagg cagcagaaga
    14521 tgctattgaa tttgatacta caggagtatc aattgaaggt gttgtaactt ttattcaaga
    14581 aaaagcagaa aaaataattg acatgaagaa ttagtcatga tatactagtt gcaatgagaa
    14641 aagcagaagt gagaacttct cgccttgcga cttaggttgt ctggcccaac atgtcaagat
    14701 tccatctagg aagattatat gtgcgggctt gatttatcaa gtccgctttc gtttttctct
    14761 aaaaaaataa agaggtgaag atcatagcta aaaaagatct attcattaac gatgaaattc
    14821 gcgttcgtga agttcgtctg gttggtctcg agggtgaaca attaggtatt aaaccattgt
    14881 ctgaagctca agcaattgca gatgatgcta atgttgattt ggttttaatt cagccacaag
    14941 ctacaccacc tgttgcgaaa attatggact atggtaagtt caaatttgag tatcaaaaga
    15001 aacaaaaaga acaacgtaaa aaacaaagcg ttgttactgt taaggaagtt cgtcttagcc
    15061 cagttattga taaaggggat tttgaaacaa aacttcgtaa cggtcgtaag ttccttgaaa
    15121 aaggtaacaa agttaaagtt tctatccgat tcaaaggacg tatgattact cataaagaaa
    15181 ttggagcaaa ggtattggcg gaatttgctg aagcgacaca agatatcgct attattgagc
    15241 aaagagctaa gatggatggt cgccaaatgt ttatgcagct tgcaccaatt ccagacaaga
    15301 aataattgtc gactttatag tattatttaa gaggagaatt taaaatgcca aaacaaaaaa
    15361 cacaccgcgc atcagctaaa cgctttaaac gcacaggttc aggtggattg aaacgcttcc
    15421 acgcgtttac ttcacaccgt ttccacggta aaactaaaaa acaacgtcgt catcttcgta
    15481 aagctactat ggtttcatca ggagatttca aacgtattaa agcaatgctt actcgcctta
    15541 aataataagg tctctgtaaa catttactac atattataaa ggattatttg gaggaaatat
    15601 aaatggctcg tgttaaaggt ggagttgttt cacgtaaacg tcgtaaacgc gtacttaaat
    15661 tagctaaagg ttactatggt gcaaaacata tcttgttccg tactgcaaaa gaacaagtaa
    15721 tgaattctta ctactatgca taccgtgacc gtcgtcagaa aaaacgtgac ttccgtaaat
    15781 tatggatcac tcgtatcaat gcggctgctc gtatgaatgg tttatcatat tcacaattga
    15841 tgcacggttt gaaattagct gaaatcgaag ttaaccgtaa aatgcttgct gatttagcag
    15901 ttaacgatgc agcagctttc acagctcttg cagatgcagc taaagctaaa cttggtaaat
    15961 aagaaataaa aaagttgggt ctgcctaact ttttttaatt actagaaaga aagtacctat
    16021 attataattt aagtgaaaag tttcgttcta tatatgtaga atgaaacttt ttttgtttta
    16081 ttccgatacc tttattattg ttataatttt cctttttgat ttttattgtt ttatagtatt
    16141 attccgtata aaaagcacct caatcgttgg attgaggtgt ggattaaact ttaaatggta
    16201 aagattattt tttagatgtt ttatgtgtct tattaggatc ttgcaattcc aagtaacttg
    16261 gagcgtggaa gagatcaact gttgatttgt tgttatgttt gctgtatatg cttgttgaac
    16321 gatctttcaa tttacgttct ttagctttga gtgctttaag agaatgtgtg taattgaatt
    16381 tttcaacttc tacagttttc aaaccattgt tttcatcaaa tcgtagtaaa tctccagttt
    16441 gaatcgaatc acttgtagat aattgtttat tagtagcatc tcgaatagtt tttatttcag
    16501 ctttagttgt ttcatcagga tttgtgattt cttgtcctga atcagtgtaa tagagatgtc
    16561 cactatagtt agtgtattta ggagtaatat actgaccagt tgttctcata gcgaccattt
    16621 gtttattatc tttagataac aagtcttgtc ctaactgagt atatttagac gtgtctatcc
    16681 ctagaatatg aagtaaggtt ggtaaattat ctacctcacc accgtatgta ttactgataa
    16741 aaccttttga atagccagga atgtggatca tatatggaac acgttgtaac atagcgttgt
    16801 cgtattcaga ccatgtttca gggtctttac ctaataattc agctaagcta ggattgcgtg
    16861 tattagaaat accgtagtga tccccataca ttacaataat agaattatca tacacaccag
    16921 cagcttttag atattcaaaa aatgatttaa gagctgtatc aaggtagttt gctgtggcga
    16981 aataaccgtt aatcgtttca tcgttagtct ttgcaagtgg gaatccctct tcgtcagact
    17041 ctcccttcaa acttgtgtaa gggtaatggt tacttactgt aataaattta gtataaaacg
    17101 gttgttgcat gtgctctaga tatttgatag agtcagcaaa catatattta tcattcaaac
    17161 cgtattggaa tgaattatct ttggtttgtt ttgagaagta gctagagtca aagaagtagt
    17221 cataacccca ctgtttgtaa gcattatttc tattccaaaa tgttccgacg tttccgtgga
    17281 agaccgcact actgtaacct ccattttgag ctaagatatg tggagcagca aattgtgtat
    17341 tttcaccacc ataattaacc atgaatgaac cactacttag accaaaaagt gagttttcca
    17401 tgagtgtctc agcatcggat gtcttaccag ctttgacttg atggaagaag ttagagaagg
    17461 agatagtctc tttcgaatga taaaggctgt taataaatgg agtaaccaca tgttctttac
    17521 catcaacatt taatttatag tcaatcaaga attgttgaaa actctccaaa tgaatcatga
    17581 taacgtttcg cccttttccg ataccataat attctggatt gggtttggca taatgctctg
    17641 ctacatattt tttggcagtc gctaattctt gtgcagtggc accatttctt tctttctgtg
    17701 cttgataagt ttgattacca ctataaatcg aaaatgatgg caagccaaga gctttaacaa
    17761 tataggtgtt tgaaaaacca cgacttagta gttctggacg atcaatttca gctaagaata
    17821 agttaattga aaagagtaaa ccagacaaag ctgtgataga aaaactagca cgtttattaa
    17881 atggacgatt atctaagtgg attttcttgg ttgcaaataa gaaaataagg ataatgaaat
    17941 cgaaaacgta gacaagatcc caaattctca atagattaag agcagagtct ccaaggccag
    18001 ccgaagtttt actactagcc aagacagcgc taactgtaat gaaatcggaa aactctctat
    18061 agtagattgc gtttgcaatt agaaggatat ttacgatagc ataggtgata aaagcggtaa
    18121 tataaaaagc tttggttcgc ttaacatata aggccaaacc tatgattaag agtcctaagg
    18181 ggatggggtt tataatggtc aaaaacaact gataggaatt ttctaaacct aaattaaaat
    18241 cagtatggta agcccaaatt gtctttaacc aatatataac tactaacaat aaaataaagc
    18301 cgagtcgtgt attgatcgca cggctagcaa aatctttaat ttttttcaca gtaggaactt
    18361 cctttaattt ttttcctaaa gataaaatct cagattaaat tataacataa aattttgtaa
    18421 catacggtaa agaaaggtcg aattcagtat gaaaaaagat aaaaatttgt tataatactg
    18481 tttatgaata aacttaaagt gaattctgtc gttgaaagaa aaatcaaatc aggtgctcag
    18541 ttactggaaa aaaaagattt tgataccagt ttagttaacc agttggttca acttttttca
    18601 cagtcaaatc aattcttagg gatggcctat ctttcaccac aaaataaagg gattggttgg
    18661 ttactatcaa gacaaatttt tgattttaac catgattact ttgtatcgct attcgaaaaa
    18721 tctagagaga agcgtcaaaa atttgaaaaa tctagccaaa caacagccta ccgcttgttt
    18781 aatcaggatg gagataactt tggtggccta actatagatt tttatagtga ctatgctctt
    18841 ttttcgtggt acaatgaatt tgtttatact aatcgacaaa tgattgttgc agcctttaag
    18901 caggtctatc ctaatattaa aggggcatat gaaaaaattc gtttcaaagg tttagacttt
    18961 gaaagtgctc atttgtacgg tcaagaggct cctgaatcat ttttgatttt agaaaataat
    19021 atcaaatata gtgtcttttt gaatgatggg ttaatgacag gtattttcct tgaccaacat
    19081 gatgtcagaa aagccttagc aactaatcta tcagaaggta aaaaagttct aaatatgttt
    19141 tcatacactg cggctttttc tgtagctgca gctgttggag gagcattaga gacaacttct
    19201 gttgatttag caaaacgctc gcgtgaactt tcaaaagcac actttgatgc taatcagatt
    19261 gtcacagata accatcgatt tatcgttatg gatgtttttg aatattataa gtatgccaaa
    19321 agaaaacatc tatcatatga tgtgattgtt attgatccac caagttttgc tcgaaataaa
    19381 aaacaaactt tttcggttac taaagattac tataaattaa ttgaacaagc tttagatatt
    19441 ttagcccctg gtggaactat cattgcatca actaacgcag ctaatctaac cgtatcacaa
    19501 tttaagaaac aattggaaaa ggggtttggt aaagcttcac ataattacat tagcttacag
    19561 cagttgcctg aagatttcac tgtaaatgat aaggaccaac aaagtaatta tttaaaagta
    19621 tttacaataa aggtaaaata atgaaaatag tagtaccagt aatgcctcgc agtcttgaag
    19681 aggctcaaga aatagattta tcaaaatttg atagtgttga tattattgaa tggcgagctg
    19741 atgccttacc aaaggatgac attattaatg tagctccagc tatttttgag aaattcgcag
    19801 gtcatgaaat tatttttact cttcgtacaa cgcgtgaagg tggtaatatt gtcttatctg
    19861 atgctgagta tgttgagtta atccagaaaa ttaattctat ctacaatcca gattatattg
    19921 attttgagta tttttcacat aaagaagttt ttcaagaaat gctagaattt ccaaatttag
    19981 tcctgtctta tcacaatttt caagagacac cggagaatat tatggagata ttttcagaat
    20041 taacagccct agcaccacga gttgtgaaaa tcgcagtaat gccaaagaat gaacaagatg
    20101 tcttagacgt tatgaattac actcgcggtt tcaagactat taatcctgat caagtttatg
    20161 cgacggtatc tatgagtaaa attggacgta tttctcgttt tgctggtgat gtaactggat
    20221 ctagttggac atttgcatat ttagattcat ctatcgcacc cggacaaatt actatttcag
    20281 agatgaagcg tgtcaaagca ttgcttgacg ctgactgata cgagataaca aaatgttaaa
    20341 tatactgatg agatccctag caattgctta gggtttttgt cttattaaaa caaacatgag
    20401 aggacattag aatgcaggtt gaagttgact taccgaatca cccttatcat attaaaattg
    20461 aagaaggatg tttttctgag gctggtgact gggtatcaca tttatggcag aaacaaatga
    20521 taactattat tacagatagt aatgtagaaa tattatacgg ggagtcgctt gtcaaccagt
    20581 taaaaaaaca aggttttaca gtacatgttt ttagttttgc tgcgggtgaa gctagtaaaa
    20641 ctttggaagt tgctaatcgt atttatggtt ttttagcaaa gcatcatatg accagaagtg
    20701 atgggatcat tgcacttggt ggaggcgtcg tcggagattt agcagcgttt gttgcttcta
    20761 cttatatgcg gggaatccat tttcttcaaa ttccaactag tttaacagca caggttgatt
    20821 cttccattgg cggaaaaaca ggagtcaaca cttcattcgc taaaaatatg gtaggtactt
    20881 ttgcacaacc tgatggtgta ctgatagatc ctgttactct aaaaacattg ggcaatcgtg
    20941 aattagttga aggtatggga gaagtcatca aatatggctt gattgatgat ataaaactct
    21001 ggcatatctt agaagagatg gatgggtcta ttgatagtat ccttgataat gcattagcta
    21061 ttatttatca ctcttgtcaa gttaaacgaa agcatgtttt agctgatcaa tatgataaag
    21121 ggttaaggat gcatttaaat tttggccaca ctatcggtca tgctatcgaa gtacatgctg
    21181 gctatggaga aataatgcat ggagaagctg ttgcaattgg aatgatccag ctttctcgtg
    21241 tggctgagcg taaaaatctt atgccaagag gtatcagtca agatatttat aatatgtgtt
    21301 taaaatttgg ccttccggtc cactatgcag aatgggataa ggacgtctta tttaacatat
    21361 tgagtcatga taaaaaagca agtggacaat ttattaaaat tgttatactg cctcaattag
    21421 gtagtgcaac agtccaccaa atacctttag aagaaatgag agattatttg gagaaataga
    21481 tgagatattt aactgctggt gagtcacatg gacctagttt aacagctatt atcgaaggga
    21541 ttcctgcggg actgaaatta tctgctaaag atattaatga agatttaaaa cgccgccaag
    21601 gtggttatgg tcgtgggaat cgaatgaaaa ttgaaactga tcaagtgatc attagttctg
    21661 gtgtgcgcca tggaaaaacc ttgggttcac ctattacact gacagtaact aataaggatc
    21721 atagcaaatg gctggacatc atgtcagtcg aagacattga agagcgtcta aagcaaaaac
    21781 gtcgtattaa gcatcctaga cctgggcacg cagatttagt aggtggaatc aaataccgat
    21841 ttgatgattt gagaaatgct ttagagcgtt cttcggctcg tgagaccaca atgcgagtag
    21901 ctattggtgc gattgctaaa cgtatcttga aagaaattgg tatcgaaatt gctaaccata
    21961 ttgtagtttt cggtggcaag gagattactg tccctgataa gctaacagtt caacaaatca
    22021 aagtgttatc tagtcaatcg caagtagcta tcgtcaatcc atcatttgaa caagaaatta
    22081 aggattatat tgacagtgtg aaaaaggctg gagatactat cggaggagtg attgaaacta
    22141 tcgttggagg agttcccgtt ggtttaggct cttatgtaca ctgggataga aagttagatg
    22201 ctaagatagc gcaagcagtt gtatctatca atgcttttaa aggtgttgaa tttggtctag
    22261 gttttaagtc agggttttta aaaggaagtc aagtaatgga ctccatatca tggacaaagg
    22321 accaaggtta catacgtcag tcaaataatt taggcggttt tgaaggcggt atgactaatg
    22381 gagagcctat tgtcgttagg ggagtaatga agccaattcc tacgctttac aagccactaa
    22441 tgtcagttga tattgatact cacgagcctt atagagctac agtggaacgt tctgatccca
    22501 cggcacttcc ggctgcaggt gtcgttatgg aagctgtagt agccaccgtc ctggtgacag
    22561 aagtgctaga aaaattttct tcagacaaca tgtatgaact aaaagaagct gtaaaactat
    22621 atcgcaatta tgttaatcat ttttaagtga aaactagtag cattcaattg caaaaaaagg
    22681 tataaaagtc tacaatagtg gtaagttcaa gtagaaaaag gagtaactta tggctaatgt
    22741 atatgattta gcaaatgaat tagaacgtgc tgttcgtgct ttaccagaat accaagcagt
    22801 tttaactgca aaagcagcta ttgaaaatga tgcggatgca caagtgcttt ggcaagactt
    22861 tttggctacc caatcaaaag ttcaagaaat gatgcaatct ggccaaatgc caagtcaaga
    22921 agaacaagat gaaatgtcta aacttgggga aaaaattgaa tccaatgacc ttttaaaagt
    22981 ttattttgac caacaacaac ggttgtctgt ctatatgtct gatatcgaaa aaattgtctt
    23041 tgcacccatg caggacttga tgtaatagta taaaagagag agctccttga gttttctctt
    23101 ttatgctatt tacattcaga gcatcctaat tgctgcgctt ttacattatt gcattatcct
    23161 ataaaaatgt atttttatac tttttttgag agcggctatt tatttttaga cttagtcatg
    23221 tctctaaata cccctagtta gtgataaaaa caaggttttt ataggataag gaggtaagaa
    23281 tattaaaata taaacatcat aaat
//
LOCUS       DATRNX010000024        23135 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRNX010000024 DATRNX010000000
VERSION     DATRNX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23135
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1041)
                     /locus_tag="VBC81_000743"
     CDS             complement(<1..1041)
                     /locus_tag="VBC81_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6911801.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPAL"
     gene            complement(1128..1631)
                     /locus_tag="VBC81_000744"
     CDS             complement(1128..1631)
                     /locus_tag="VBC81_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6911802.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(1717..3048)
                     /locus_tag="VBC81_000745"
     CDS             complement(1717..3048)
                     /locus_tag="VBC81_000745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6911803.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(3058..3675)
                     /locus_tag="VBC81_000746"
     CDS             complement(3058..3675)
                     /locus_tag="VBC81_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911804.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(3840..6938)
                     /locus_tag="VBC81_000747"
     CDS             complement(3840..6938)
                     /locus_tag="VBC81_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6911805.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(7093..8403)
                     /gene="der"
                     /locus_tag="VBC81_000748"
     CDS             complement(7093..8403)
                     /gene="der"
                     /locus_tag="VBC81_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6911806.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(8451..9353)
                     /gene="dnaI"
                     /locus_tag="VBC81_000749"
     CDS             complement(8451..9353)
                     /gene="dnaI"
                     /locus_tag="VBC81_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6911807.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(9350..10525)
                     /locus_tag="VBC81_000750"
     CDS             complement(9350..10525)
                     /locus_tag="VBC81_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6911808.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(10525..11004)
                     /gene="nrdR"
                     /locus_tag="VBC81_000751"
     CDS             complement(10525..11004)
                     /gene="nrdR"
                     /locus_tag="VBC81_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6911809.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(11153..12658)
                     /locus_tag="VBC81_000752"
     CDS             complement(11153..12658)
                     /locus_tag="VBC81_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6911810.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIS
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(12648..13337)
                     /locus_tag="VBC81_000753"
     CDS             complement(12648..13337)
                     /locus_tag="VBC81_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6911811.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(13572..14105)
                     /locus_tag="VBC81_000754"
     CDS             complement(13572..14105)
                     /locus_tag="VBC81_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6911812.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(14210..15100)
                     /gene="htpX"
                     /locus_tag="VBC81_000755"
     CDS             complement(14210..15100)
                     /gene="htpX"
                     /locus_tag="VBC81_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6911813.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(15121..15675)
                     /locus_tag="VBC81_000756"
     CDS             complement(15121..15675)
                     /locus_tag="VBC81_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6911814.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            15803..16516
                     /gene="rsmG"
                     /locus_tag="VBC81_000757"
     CDS             15803..16516
                     /gene="rsmG"
                     /locus_tag="VBC81_000757"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6911815.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            16531..17910
                     /locus_tag="VBC81_000758"
     CDS             16531..17910
                     /locus_tag="VBC81_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6911816.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLSHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            17923..18594
                     /locus_tag="VBC81_000759"
     CDS             17923..18594
                     /locus_tag="VBC81_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6911817.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(18629..19459)
                     /locus_tag="VBC81_000760"
     CDS             complement(18629..19459)
                     /locus_tag="VBC81_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6911818.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(19452..21128)
                     /locus_tag="VBC81_000761"
     CDS             complement(19452..21128)
                     /locus_tag="VBC81_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911819.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(21145..21693)
                     /locus_tag="VBC81_000762"
     CDS             complement(21145..21693)
                     /locus_tag="VBC81_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6911820.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(21862..23070)
                     /gene="sstT"
                     /locus_tag="VBC81_000763"
     CDS             complement(21862..23070)
                     /gene="sstT"
                     /locus_tag="VBC81_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6911821.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
BASE COUNT         7373 a         4643 c         3506 g         7613 t
ORIGIN      
        1 taaagctggt tttgtcggtt ctgcagttcc gcctttttcc aattcagcag caatctgatc
       61 taaagtcata ctttttttaa gattgtagta tccactttga aaattactat agtttttgaa
      121 cttggaatag taattaaaga ctgtggcgct cttaatgact cctgcttttt ctaaaatttg
      181 accaattaat ttattcccag atcctatcgg aatttcaact tgtacaaatt tatttgaatt
      241 ggaatcaaca ggtttaatag cactatctac aaaacgataa cctacaaatg ctgtcaagag
      301 caatacaagt atgataattg atatcagaat acgtgcaatt cgttttgcca cattatctgc
      361 tttctgacgt ctttcacgct tgttatgttt gttcttggct gacaaagcta taccctcttc
      421 ttcttttgac aaatcctcta ctcggatagg ttcctttgct acgggctcta actctgatgt
      481 ctcaccaagt tttacatttt caccttctgt aggaatatct gttgctctca caagaaagat
      541 ttcttccgtt tcttgattag gcacagcagt tgcaacatca gtcttatctg cttttaaatc
      601 tatgtcaggt acattttcag ttgtcctaaa ttcactagat ttggagccgc tatcaagctc
      661 tcttgaaggt tccatccctt gattgataac ttgttcttct tgggaagcta atgcttgttc
      721 cgcctgacgt ctttcttctt ctcgagcagc ttctgccaga agtttcgcct caatccgagc
      781 cttctcttgc tcttctaagc gttgcttagt ctctagcgcc tttgctctag cttcttgttc
      841 gtctttcaaa cgttgcgctt cataatctgc catcaattgt gctgttctgc gagcagcttc
      901 tttggcttct tgctcttttt gatagagttc ttcttcacgc aactttctca aacggttagc
      961 ttcttctaac tctgccagaa tttgttcttt aaaacttttc tgatcatggt tagaatgctg
     1021 atcgtcatta aattcggtca aagagatttc ctcctaataa taatcacagg caattatatc
     1081 ttaaatagtc tataaaatca acaaaacttt gacaatgtta tctactttta acaaattgat
     1141 cgtgttggat tattccaaat catattatac caaagcgaac caccatgttg ggagtcagat
     1201 tctccctcat ctttaaatcc attcatttca tagaaggaaa ttaaatcatc atggcaagtt
     1261 aaacttatac catctcgttc ttgagaaaca accaggtctt tcatcgctgc taataatgct
     1321 gtaccaatcc cctgaccttt aaaatcagga tgtattgata gactagtaat tcctatgaaa
     1381 ccacctaccc ttacagggaa ttcagaaact ttatggaata aatcgtccgt taagtaccga
     1441 cctttaataa ccggtccttc aatgtaacct gctaaacgac cattaatctc tgcaactaaa
     1501 aaagtatcag tcattatcgt taaacgttct tgcattgctt ttttactcgc agcctctgct
     1561 tctgaaaaat ttatttgttc aatactaatg acttgctgta aatcctctag gcaaccattt
     1621 ctaataatca tactcatctc cttaatttct ttatgaaatt ctcttgataa agtcagatag
     1681 aggactgaga aacttctctc agcccttacc atcaacttat tgtgtatttt tagttaggtt
     1741 agctaataat tcttcaaaag agcgctcata caattgaatg tctccagcac ccataaagac
     1801 atagacagca ttatcatgat tgagtaaagg cgagacattt tcgactgtca ctaaatctga
     1861 gtgtttgaca atcttagcag ctaaatcttc taccttcacc tcaccattat ctacttctct
     1921 agcagaacca tatatttgag cgagataaac gctatccgct tgactcaagg catgggcaaa
     1981 ttcgtctaaa agagctatcg tacgagtgaa cgtatgcggt tggaaaatag ctacaatttc
     2041 ttttgacggg tatttttgtc gagcagcatc taatgtcgca ataatctcag taggatggtg
     2101 agcaaagtca tcaataatga cagtatcgtc aataatcttc tcagtaaaac gacgctttac
     2161 ccctgaaaat gtcttcaaat gctcagctac taatgccata tcaattccca ttatgtaaag
     2221 gttagcaata acagcagttg catttaagat attatgttta ccgtatgctg gtacatgaaa
     2281 ctgaccaatt tcttcttggt tatagaaaac cttaaagtca gaaccattaa cagttcgagt
     2341 gatgtctttt gctataaaat catttgaatc ttcaaaacca taataatata ttggtgcctc
     2401 agaagtgatt tcatgaagtt ttggatcttc tccataaatg aataaacctt tttgaacttg
     2461 cttagcatag tcattaaagg cattgaatac gtcctctagg cctgtaaaat aatcaggatg
     2521 gtcaaaatca atattggtaa taattgagta ttctggatgg tacggcataa aatgacgttc
     2581 gtattcatca gcttcaaaca caaagtaatt agcattagca gaaccacgtc ctgtaccatc
     2641 tccaattagg aaagaagtgt ctgtaatatt ttttaaaaca tgagctaata aacctgtcgt
     2701 tgaggttttt ccatgtgccc cagctacacc tagactagtg aactgacgca taaaatctcc
     2761 gagaaattca tgatatcgtt taaaatgata gcccttttca ataacataag ccaactcttc
     2821 attgttatct ggacgaaaag catttcctgc aataatctct aaatcctcac tgatattatt
     2881 cggtgagaaa ggtaatatag ttacacctgc ttgctctaaa ccacgttggg taaaataata
     2941 tttgtcaacg tcacttcctt ggacgttatg tcccatttga tgaagcatca gtgctagggc
     3001 actcattccg gatcctttaa taccaataaa atgataagtt tttgacatgg tctctcctta
     3061 attaatgtct ttaaaacgtg tcagatttaa ttcttgagcc ttggctcttt ctttatgaaa
     3121 ctgattgtct tgtttattgt aaacttgact gcgtttcaaa aaatcaaaat tatttttctt
     3181 tgtttttggg ttcggtgtat tatcaggttc ttgaaatatg attggaattt ctgctaatat
     3241 atagctttcc tgctgtaaac gatcagaaag atgacttagt tcagtcacct ttcgctgagt
     3301 ttgttttttc ggtactgttg ccttcaattg ttgctgaaaa gcttcctttg atggcgcctt
     3361 aaattcctgt ttcgcatagt aagcttgtcg tttctcccga acagacctct ttgcctcttg
     3421 tcgagatttg ctcaactctg tcgctttttt tacaaaactc tgcggtggta caggatcatt
     3481 ttcaattacc ttaggatgtg atatttcttc atgttcataa ttgcttttga ccatgctttg
     3541 atatgttttt tcttgataaa agcctcgaat gttgttaatt aaatcttcat tatcataaag
     3601 attcatcttt acaagcggag acattactgg ttcatcatct gctatcaacg gaaatcgtct
     3661 agagcctcta ttcacattat cacttccttt ctacaacaga cctattataa actaattttt
     3721 ctgttttttc aaagttttca actaagtaaa gcttatcaga ttggtatttt tcattcattt
     3781 atataaaaaa gaaagcgaga gaccactttc ttttttatat tatagtcaca tcatcatatt
     3841 tatttagaaa ctccaagtat ctctcgaata tcatctacgc tcatgctagc atgggtttca
     3901 tttccatcca gaactgttgt aaccaaatgt tttttagttt cttgcatttc tagaattttt
     3961 tcttcgattg tgcctctagt aatcaagcga taaacctcga cattctcttt ttgccccaac
     4021 ctatgtgcgc gactaatcgc ctgcatttca acagctggat tccaccacaa gtcaatcaga
     4081 acaacggtat cagctcctgt aaggttgaga ccaacgccac cagcttttaa agaaatcaag
     4141 aaagcatctt ttgatcctgc attaaatgct ctggtcattt catgacgttc atttgctggt
     4201 gtagaaccag taattttata agttgtaagc cccatagcta ccatctcacg ttctgcgata
     4261 tctaacatac ccctgaactg agaaaaaatc agagcacgat gtccattttc cttaatttgt
     4321 gttaaaagtt gacgtaggct ttctaactta ccgctttcac catcataatc cataaaaaga
     4381 cgtggagtgt cacaaatttg acgtaatcgt gtgatgccag ataaaatttc aattttacga
     4441 cgatttaagt cagcgtcact agaatgttgg atactttctt gaatttggcg taattgcgcc
     4501 aaatagataa ctttttgact gtctgtcatt tcatttggat agttcatttc aatcaaatcc
     4561 ggtaattctg ggagaacttc ttcttttcga cgtctcatca caaatggttt gatataacga
     4621 gcaacttgtt tagggttcaa cttcaaaaac tctttcttac caggtaataa accaggtaaa
     4681 atgatctgga aaatggacca aatttctaac agtttattct caataggtgt tcccgataat
     4741 gcaaaacagt ttttgacctc aaaagatctc aagctatggg ctattttagt ttgagcattt
     4801 ttcataacct gtgcttcatc caaaattaaa taatcataat ttccttcaga ataagtctca
     4861 aaatcctgcc taaatgatga ataacttgta atgattactt gatgacgttc agcaataatt
     4921 tcatctctac gaatcttaga accataggca actgctacat ctacgtctgg cgcaaatttg
     4981 tggaattcat cttgccaatt ataaattaaa cttgatggtg ataaaataag cacacgagaa
     5041 tctctagtta gttttgtact taaaaatgaa atcgtttgaa gtgtttttcc aagccccata
     5101 tcatcagcta aaatccctgc aaagccataa tgatctaaca tacttaacca acggacacct
     5161 gtaagctgat aatcgcgcaa ggaagctgaa acagatagac caggtatttt aaacttttct
     5221 gggtgtctca aatcttcaat caattggtaa aaatgttgtg aaaagctaac attatcttga
     5281 tttgcaaaca gttctgataa ttgaaatgct gcaatattat ctaattcaat atgcccattc
     5341 tttgctcgtc tagctctcaa cccttgcaaa gttgcactta ctttcttagt ttcttcatca
     5401 aaaataacta attgccctga tttattcaca aaataaggat tattgtcaaa caaagctacc
     5461 agagcttgat caacatcgtt ttccagtaaa tcagagaaat caaaagaaat atctagcagg
     5521 cttcccttag cttcaatatc aatttgcggt cgttctatga gacgataact ttccaatttt
     5581 gcggataagc tcactgttcc cattttttta aattgtggaa gcatataggt ataaaaatcg
     5641 tataattctt gtgcactgta tgctggatgg ctggttgaaa agtggggtgc aaagccatat
     5701 ttttctaaga gtttaaatac tttttcttca tgtttgaagt gactagcaaa tgttaactgt
     5761 tctagttctt gacgattatg aacagttaaa tcattaccat aatcaaatac catttgcaat
     5821 aaaatttcat tttgactatt aatatcgaac tcaaaattta ccttaaaatc atgaatagag
     5881 aagctccgtg gagcatccac tagtccaatc tccttaaaat ccagtaaatg agctgctaac
     5941 ttatcttgat catctaaatc aaaatggata tgttttgcta aatctccctc aatgggaaga
     6001 ctacgaatag ccgttaccat cttttcttgc ttcaaattaa gatggtagaa agtatcgcga
     6061 taaaacaaat aagaattcgc aaatagtggt tttaagtctt tctctttgat ttctaattca
     6121 attgattgcc tatggacaat aaccttaaat tgataaagat gatcctcacc ttctaattcc
     6181 ttaaagtaga ggtgattaaa ttgatgaaaa tcactttcaa atgaaaaatt ttctaaagca
     6241 ttcatcaaag taacaccctc ttcaaaaaaa ccacgagtga gactaaggtg acgaccttga
     6301 tttggtaaaa taaactctag atcaattttg cttgaatgac ttggaatgag cctccataaa
     6361 aattctatca attcttgact ggtctcatca aactgtatca aagacaaagt ttcaaagtat
     6421 tgttttccaa tctgataata agcttctttt cgaacagtat ttaaaaaggc tttgatgtca
     6481 cgaataacat aacttctatc atcaggcagt cgtctaattt ttaaagtcca ccagatatca
     6541 ttagcataag gattttgttc tccttgagcc gataattgat attttatagt atcatcttca
     6601 ttaataatta gactatctaa aaaaacacta ccaaaagagg ttttcttctt agtttcttgt
     6661 tgactttctt gttgaacttg aactttagat aagattgctt taccttctgg atcgtttttc
     6721 aagaaatgct ccagagcagc taaatgttga caatagcctt ttcttgcaaa aaaatcacaa
     6781 gaacattttg tttcttctgt tactaatgaa tattcaatct gacaatcccc aactttagct
     6841 tttaatagat taccttcttg agaaatcaag gacaccaaac cttgttcgta gagttctata
     6901 ccttgatttc gaatacgtcc tggaatcatt ctactcatta tgctaccacc ttcatcttat
     6961 cccttaatta taacaaaatt caagtccaat ttcttgtaaa aatactaaat tgattgtttt
     7021 ttgatacagt aacaaataaa aaaggctaaa acaactgtcc taaccttcta tttcaatttc
     7081 aacaataaga tgttatttac gtttacgtgc tattagatta attggtgttc cttcaaaaac
     7141 aaatgcttca cggatttgat tttctaagaa acgaagatat gaaaagtgca tcaattcttc
     7201 ttcgttaaca aaaacaacga aagttggtgg tttaacagca acttgagttg cgtagaaaat
     7261 cttaagtcgt tttcctttat cagttggtgt tggattaata gcaatagcat ccataatcac
     7321 atcatttaat acagcagacg gaatacgttt gttttgactt tcgctaatac gtttaatcat
     7381 atctggtaac ttgtgcaaac gttgctttgt ctctgcagaa acaaagataa ttggcgcata
     7441 agacaagaat tggaagttat cacgtatatc agcttcccac tgactgacag tatgattgtc
     7501 tttttcgata gtatcccatt tattaactac aataataata cctttaccag tttcatgtgc
     7561 aaaccctgca atacgcttgt catactcacg aataccttcc tctgcattaa tcaccataag
     7621 aacaacatct gagcggtcga tggcgcgcat agaacgcata acagaatatt tctctgtgtt
     7681 ttcatatacc ttaccagatt tacgcatacc agcagtatca atcatagtat actcttgccc
     7741 ctggctatca acgaaattag tatcaatagc atcgcgcgta gtaccagcaa ctgggctagc
     7801 aataacacgg tcttccccaa ggatggcatt aattaaacta gatttcccaa catttggacg
     7861 tccaatcaaa ctaaaacgaa taatatctgg attttcattt tcctcttcta ccggaagatt
     7921 ttccacaata gcatccaata tatcaccagt accaatacca tgaactgatg acaagggata
     7981 aggatcccct aaaccaagag agtagaaatc gtaaatatca ttgcgcatct caggattatc
     8041 taccttatta acagctaaaa taaccggttt attggtctta taaagaatac gtgagacata
     8101 ctcatctgca tcagtcacac cttctttacc cgacacaaca aacacaataa catcagcctc
     8161 tgtcatagca atgtctgctt ggtgcttaat ttgctccata aacggtgcat caacatcatc
     8221 aatcccacca gtatcaatta atgaaaactt acgatttagc cactcacccg ttgtatagat
     8281 acgatcacga gtgactcctt caacgtcctc aacaatggaa atacgttctc ctgcaattct
     8341 gttaaataat gttgattttc caacattcgg acgaccaaca atagcaactg taggtaatac
     8401 cataattctc ctctcactat tgggaatact cccaattcca ttctatttta ttaacggcga
     8461 ttcggtcctt ctaagtggaa ttctatcgct aaataccgta cgcgctccat aactcgtttt
     8521 gcttgccaag tttcgtcgct tccttttata ttagcccatt ttctttccaa atcgttaaaa
     8581 ctataatttg acgtaaagaa agtgggcaac tcttctagca tacgatgttg taatataact
     8641 tgtaaaatct cgtcacgaac ccaggacgtt gcctgttcag ccccaatatc atctaaaatc
     8701 aaaatcggaa cacttttcac agcatcaatt tcatctttta cagttccgct tgaaatagca
     8761 tttttgacat caatagcaaa actagggaaa tgaagaagag tcgttgacac accctttctc
     8821 tctgataatt ccctcgccat tgctgccatt aaataggatt tcccaacccc catatctcca
     8881 tataaataaa gccccttgtg attatatgaa ggataagtct ctacaaaatc aagcagttgt
     8941 gacatagctt tcattcgact ctcgttatta atatcaaagt cagtcattct gatattccga
     9001 taggacttgg gtagattaac taaattaata cgatcactga tagcttgttt tttttgtgct
     9061 tcaattaact ctcgtgtttc taaataagaa acatctgcat aaccttcatt cataaccaaa
     9121 ataggctcat agcctttagc aatatattga ctatccttat ttttaaattt ctgacgctca
     9181 atcaaaaatt gattaaattt agacatgctg atatttattt cacgctgaga aagttggtgg
     9241 gttttaatga actccgctac ctgagcatct gctaaaatca tttgaataag ctcatcattt
     9301 gtattacgag gcactctccc ttgattttct aatgcttgtc ccactgattt catgattcac
     9361 catcctttcc taatcgttct aaacgcttga gggcatctgt tttaaactgt tctaattcaa
     9421 tttctttttc tgggctaacc tcatctttat aatcaggatt actccattca ggaacattcg
     9481 attgtttctt cttcgtttct gttttagtcc ttactttttg atctgaaaaa tcacgaattt
     9541 taagcacagc atcttcggcc gtcataacat tctgaaaggc aaaatcatta gcaactttca
     9601 tgatataggc cttatttaag ttagcagatt ttgtcttatt caaggtatat aaaaccataa
     9661 cattaatgac ttcgtctagg aaattcattt ttgccaaatc ctcaagtaaa gtcttctcac
     9721 cacttgttgt cactgcctta cggcttctct tgattttttc aaggaacact agagcactat
     9781 catttttaga ttcccttagt atgacctttt cattatttgt aaaactctga ttatctttaa
     9841 tatgctgccc ctcattttgt tgaagtttca ttcgctgagg cgcaatcatt ccattgatag
     9901 ctgtttgttt tgctaattga taggtatcat accaatttaa atgataaagt tctgatacac
     9961 tataaatacc taagacatca tctttttcat tgttaaaacg taagccatct cgcatcatca
    10021 aacgtttgaa actttccaag tcaaaaacat ttttactccg gtttacctct tgttttggtt
    10081 gtcctaaatc cgagaaaaca tctgtaaatt ttttagagat atctctagca tttttaggta
    10141 ttttcatatc taattctgcc actgctacct ctcctatacg tttttctaac aaacgacgat
    10201 agatgggatt agataaaaat aactcatttg acatagcttg attcaactta acaaggtaag
    10261 tacctgtggc ttggtaaaca gtcaacaaat ccaaagctgt taacattacc aatgcatctt
    10321 gaaatcttgg catcccgtat tgcagatgat tgagaacctc tgaaaattta tgacgtcgta
    10381 ttccgtcatc aaaaaaatga acaaaataat catagagtgc catggcatca cttcccataa
    10441 ttgggaaata caattgtatt aaactattac tatcataaac aatcttattt tgcttaatat
    10501 atgtaaactc atctattgga ctcatcacac gctaccactc tttttactcc gtacgcgctt
    10561 tgtaatttgt tgaagtagtt cttcaatttc atctacatct ttaaaagatt tataaacact
    10621 agcaaagcgc acataagtaa tctcatctaa ttcggcgagt tcatccatca ctaaatttcc
    10681 gatagtgata ctagaaacct catcttcata ctcactccta attttacgtt cgatacgtaa
    10741 gatacaattt tcaatatctt cgctcgaaac agggcgtttt tgagcactct gaataatacc
    10801 attcaaaatt ttatctcgag aaaattgctc gcgagtacca tctttcttta tgactaaaag
    10861 aggcaattct tccaaacgtt caaatgttgt aaagcggttg ccacattttt cgcactctct
    10921 ccggcgtctg atagtcgtac cttcttcagc ttgtcggcta tcaaccacac tagatttatt
    10981 atagccacat tttggacaac gcatgtcaac acccctattc tatcatatca tttaatcatc
    11041 ttattttaac acaaattcag taataacaaa agaagcgcct ctcttatcac ctcatcatat
    11101 caaaacacaa aataatcctt atgcctttta tcattaagca taaggatttt tcttatattt
    11161 ctttagtttc ttcaaatttt tttataggaa tgcgaacaat gaattcagtc ccttgattta
    11221 gttcactttt aatatccacc tttaaatggt aagcatccat aatttgttta aacactgaaa
    11281 gcccgatacc taaaccagct tgtgtacttt ctctgttacg tgatttatca gttcgataaa
    11341 aacgatcaaa gataaactca atatcttcat ctgagatacc ttctccttta tcttttacaa
    11401 ctactgtcgc aaaatcattg tctactgata aaacaacact gacttcttta taactaggag
    11461 agtacttgat agcgttatca attgagatca tgagagcttg ctcaaaatgt atcttatata
    11521 tctttcccca aacgatatct gaaatattat tttcaaaaat aaattgaaaa tcttctctca
    11581 aaatacggaa attttcaata acaactgata tagagcttga taaatctgtc acttcatctt
    11641 gatgaagttc caagcttcct tggacgcgaa tcatatttaa catgtcatta atcattaagg
    11701 acatacgatc tgcctcatgg tatgccgctg ctaaactttc ttcaagaatt tctggatcat
    11761 ctttccccca acgttgtaat aaaccaatat gaccctttac aacagcgaca ggcgttctta
    11821 attcatgact aacatcacta atgaattgtg attgtcgacg agtatagtct tccaactgat
    11881 ccaacatccc atcaaaaatc ctagacaatt ctccaatttc gtcttctgaa cggaccttac
    11941 ttctaatttc taaattacta ggattttcag atatctgatg cattaagtcg tgtaaatttt
    12001 taaccggacg aacaatacgc ttagtagcac tattaataac gaccaaggct aaaacagtcc
    12061 ctaaaacttc catcataatc aagaaaacaa tgatgttttg cctcatacta taataacgac
    12121 ctaaattata gaaaatttga acgtatccaa gcaactgtcc tgttttttga gagtagattg
    12181 cttttgtacc aacaaaacct tccatatctg caactttaga gatgataact tttcccatgg
    12241 atttaccaat agttggcata cccgttctca gtgtagaaaa aactggtttc ctgttgacat
    12301 cgtatagggt catttcttga ttaacataaa gaatactatc gattgactcg cccgttctaa
    12361 caattccatt ttgacgctta tgaggataga tgtcatccgc aaaaacctgg ttgtacagga
    12421 tctctaaaat atcatcggat gtcaaactag agttcgcttg cgacaaacga actctagttt
    12481 tttctaatga cctaccgaca ttaatttttt ccctgtgaag gacgtatctt gttgccgaag
    12541 tataaactaa aacagaaaat actgtaaata ggctaaaaaa taagataaaa actaattgcg
    12601 ataatttttt tgacagaggt tgttttaccc ctataaactg atctttttta tttttcacga
    12661 atcacatagc ccattccgcg aacagtttga atatatgatt cacggcctgg gatatcaatt
    12721 ttacctctta ggtaacggat ataaacatca acaacgtttg tctctgctgc cacatcgtat
    12781 ttccaaacat gttctagcaa ctcttctcgt gtcataacac gattcatatt tgtcatcaag
    12841 acattcaaca aatcaaattc acgttttgtt agtgaaattt cctcatcacc acgcatcgct
    12901 gaacgattat gagtatttaa tgacaaatca cggaaactac cactatcacc tttttctttt
    12961 gtcttcgttt caatttcttg gcgtcggaaa atcgctctaa cacgtgctaa taattcttcg
    13021 attgcaaacg gcttaacaat ataatcatct gctccacgat caagaccagc tacaatatcc
    13081 ataacagaat cacgtgctgt catcatcatg atataggttg ttttttcagc ctgcaggcga
    13141 cgtgtgattt cgaaaccatc catctctgga agcattaaat ccagtagaat caaatcaaaa
    13201 tctttttcta atgctgtgtc caatccttca cgaccgtttg tttcaacaac aacatcatat
    13261 ccttcatgta gtagttctaa cgagacgaag cgagctaaat ttttctcatc ttcgattatt
    13321 aagatctttt tacccatcta caccaacctt cttattcttt acttaacact atcaattatt
    13381 ttttagaata tctagataaa aaacaagata gcccacattt tactaacgtt tctttaaata
    13441 aataaatcgc catcccaaac aattgttcga tagcgagact taatttttat tattgagtag
    13501 tgtcctctgt cacttttcaa ttctactaaa aaaaaattaa aatatcaact gatgttatta
    13561 ataattttta tctaatcact atcaaacata ccttctaagg ctgcgaaagg attagatttt
    13621 tctttttctt tagcttgttt ttcttcatac tgcttttcag tcattaacga ccaattttta
    13681 cctgataaat ccgcttctac cccaacctca tcagctgcca gaacacgaag aggtatattt
    13741 aacaagatat tatcgataac gctttcttct aaattaatat catcttcctc taatattaag
    13801 accaactcct gatcaacgag ctctttttta gtgctaacat tttcagcttc aataaaaact
    13861 tcattaatag tttgcttttc agataaaaca actggtttca tagaacgact agacggtaat
    13921 gtaatatcgt aagccatatt atagtcaagt agatagagac catcttcata caccacatga
    13981 cctgaaactt gaatatcaga aatatccatt atctctgaat gacgctccat aagcgactct
    14041 ttgatatcta tttttttatc aaaataaagc ccctctggtg attttttgat ttctgtaagt
    14101 aacacgtgcc atctcccttt atagtatatg tcatattata tcaagtttta caggctttgt
    14161 cttaaaaaaa caaaacgcaa ggcatagcta ccctacgttt tgttaacgac tacatatgac
    14221 gcaaacgctc aatacgatct gcaattggtg gatgcgtata aaataaagac tttaaccctt
    14281 cttttttcgt tgggtcattg atatagagag ccgcactagc atcatctact ggatgcccca
    14341 taggttcaga acgatcaagt ttttctaaag cagagatcat tccttgtgga tttcttgtta
    14401 actcaacaga actagcgtct gctaagtatt cgcgctgtct tgaaatagct aattgaacta
    14461 aactagcggc taatggtgca aggattagtg ataaaataga gaaaatcaaa accaagatgt
    14521 ttccaccatc ttctctgtca tcatcacgcc tacgacctcc gccgtaaaat aacatacggc
    14581 taccaatgct tgatattaga gttacagcac tcgctaaagc tactgcaatg gttgaaatac
    14641 ggatatcata attacgaata tgactaacct catgtccaat aacaccctct agctcttcgc
    14701 ggttcataac agctaatagg cctgtcgttg ctgcaacagc agcattttct ggcttagagc
    14761 ctgtggcaaa ggcatttagg gagtcatctt caacaatgaa aacgcgtggc ataggtatct
    14821 gagctatcat tgccatatct tcaacaatat gaaaataatt tggcgcctca tcttctgtaa
    14881 cttcacgcgc attgttcatt gacataacaa cattagttga ttgaaaaatc atacttactg
    14941 catagataac cccaataatt agagctagaa caagcccaaa ttggtagctg cctaatacca
    15001 aataacctac agctgctcca atagctgcta aaagacagaa gaagacaatt aacaagacaa
    15061 ctgtttttct cttattactt gcaatttgtt gatacagcaa aagcctctct cccttctcaa
    15121 ttaaaagtca aaggaaactt ttggcacttc tttttcttct tcaggagttt ctaagaattg
    15181 gcttggtttg aacccaaata gttttccaac aatattactt gggaaagttt ctaatttcac
    15241 attataattg gatgttgttg tgttatataa ctgacgtgaa taagaaattt tattttccgt
    15301 attagttagt tcttcttgaa gtttaacaaa gctattgttg gcttttaaat ctgggtaatt
    15361 ttcagcaacc gctaaaatac ttgaaagctg tcttgttagc tcattacttg ctgtcattgc
    15421 ttcagcaggt gtgttagctt tagcaacttg agcacgcagt tctgcaatct tctcaagtgt
    15481 tttcccttcg tacgcagcat agcctttaac agtttcgatt aggtttggaa ttaaatcgtt
    15541 acgacgtttc aattgaacgt ctatttgact ccatgattct ttagtatgca tgcgacttct
    15601 aactaaactg ttataagcaa caatcaacca aataacaaac aaagcaataa ttgctattaa
    15661 aatcatagtt cccatagtag tagctccttt acattttttc cattatagca attttcttta
    15721 gattaggaaa gtcctgttat tcccattatt agtatttgtg ataaaataga gtcattatac
    15781 gatatagaaa ttgaggttta ctatgacacc ccaagcattt tatcaagttc tgatagagca
    15841 tggtattaca cttactgaca aacagaaaaa acaatttgaa acttatttta gacttcttgt
    15901 tgaatggaat gagaaaatta acctaacagc tattacagat aaggaagagg tttacttaaa
    15961 gcacttctat gattctattg cgccgatttt acaaggttat attgacaata gtcctcttag
    16021 cattttagat attggtgctg gtgcaggttt tcctagtatt cctatgaaaa tcctttatcc
    16081 tgaaattgat attactatta ttgattcact gaataaacgc attaattttc taaacatatt
    16141 agctaatgaa cttgaacttt caggagttca cttcttccat gggcgcgctg aggactttgg
    16201 tcaagataaa gtatttcgag ctaaatttga tatcgtaaca gctcgtgcag tagctagaat
    16261 gcaggttcta gcagaattga caataccttt tcttaaagtt aatggtcgct tgattgcact
    16321 gaaagctgca gctgctgaag aggaattaat ttctgcagaa aaagctttga aaacactatt
    16381 ttcacaagta actgtaaata aaaattataa gttacctaat ggtgatgacc gaaatatcac
    16441 aatcgttagc aagaaaaaag aaacgcctaa taaataccct cgtaaagctg gtactccaaa
    16501 caagaagcct ttgtaaagtt gggagctagt atgaaacatt tctttgatta caagacaatg
    16561 tctgtcgcac gtaaactcag cattagtttt atcgctgtta tcctattggg aagtatttta
    16621 ctttcacttc caatttttca atatgctaac gcccctaaaa cacattacat tgatcacctt
    16681 ttcacgactg tttcaatggt atgtgtaact ggtttatctg tttttcctat cagtaaggtt
    16741 tataacggtt gggggcagat tgtagctatt ttactcatgc aaacaggggg acttggttta
    16801 gtaacactaa tgtcgttgtc ttactatacc ctaagacgta aaatgtcgtt aaatgatcag
    16861 acccttttgc agtcagcgat aacttataac agttcgacag atttaaaaaa atatctctat
    16921 atgattttta aagtaacttt aactttagaa gttttagcag ccagcatttt agccatagat
    16981 tttattcctc gatttggttt gagtcatggt atctttaata gtatattctt agctgtctca
    17041 gccttttgta atgccggttt tgataatcta ggggcaacga gcttagctca atttaaacta
    17101 aatcctttgg ttaacattat cgtttgtttc ttgattattt caggaggact tggatttgcc
    17161 gtatggaaag atttaattga agctactata caaacatctc acaagggacc aaaattaatc
    17221 aagacatttc caaaacgcct cagcaaccat tctaaactgg ttttaaaaac aacaaccatt
    17281 attttactaa caggaacttt gctttcttgg ttgttagagt ttggaaattt tagaacaatt
    17341 gctaatttat ctctacctaa acaactaatg gtcagctttt tccaaacagt tacgatgcgt
    17401 accgctggat tttcaactat tgattatacc caaactgatt tcgcaaccaa ccttgtttat
    17461 attatccaaa tgttaattgg aggagctcct ggtggaacgg caggaggatt taaagtcact
    17521 gttatcgcca ttttactctt actatttaaa gccgaactga gtggccaaag ccaagtgact
    17581 tttcattatc gaacaattcc ttccagtatt attaaacaaa cactatcgat tttaactttc
    17641 ttttttatca tacttattag cggctatctc cttcttctgg aactcaatcc ccatatagat
    17701 cctttttctc ttttctttga ggcatctagt gccttagcaa ccgtgggtgt aacaatgaac
    17761 actaccaatc aattaactct gggcggtcga attgtaatta tgttcctcat gtttattggt
    17821 cgagtcggtc caattaccgt actgctatca attcttcaga aaaaagagaa ggaaattcac
    17881 tatgctgaaa cagaaattat tctaggttag aaaaggtctt ttatgaaaac aaaaattatt
    17941 ggtgtacttg gtctaggaat atttggacaa acactcgcac aagaactaag taactttgaa
    18001 caagatgtta ttgctattga cagcaatcct gaaaatgtac aagctgtcgc cgaagttgtt
    18061 acaaaagcag ctatcggaga cattactgat ttagctttcc taaaacacat cgggatcagt
    18121 gactgtgata ctgttattat tgctacagga aacagtttag agagctcagt attggccgta
    18181 atgcactgta aaaagttagg cgtcccacaa gttattgcta aagctcgaaa ccttgtatac
    18241 gaagaagtac tttatgaaat tggtgctgat ttggttatct ctccggagcg agaatctggg
    18301 caaaatgttg ctgcaaacct catgagaaat aaaattacag atgtcttcca gattgaatct
    18361 gatatttctg tcattgaatt taaaattccc aaatcatggg tagggaaaac tgtcgaacaa
    18421 ttaaatatcc gtcacaagtt tgatcttaac ctcataggta tcagaaaagc aaaaaacaaa
    18481 cctgtcgata ccgaagttcc aatcaatact ccacttgaag aaggtattat tctagtggct
    18541 atcgctaact cagatgcctt ccaacgctat gactatttag gatactttaa ctaaagaaaa
    18601 taaaaaactg actattaaag tcagtttttt attgccatgg gttatagaaa cgtccatgat
    18661 taaggtgaat taaataaagg cttacaaaca gactagctag agcaagtatg attaagacaa
    18721 tgtcactttt ttccaaagat tgcttagagt accaagtacg tcttttattt ttaccaaatc
    18781 gtcgaagttc catagcagtt gctactgtat caatacgttc tagagagcta aaaattaatg
    18841 gtgtgacaat ctgcaaattc cccttaatgc gtgctactaa gttcgatttt tttgataatt
    18901 ctattcctct tgcctcttgt gctcgacgta ttgtataaaa ttcttcttga acatcaggta
    18961 tatatctaag ggtcaaacta acagcataag ctattttata tgataaacct aattgattta
    19021 aacttgatgc gaactgacta ggattagttg tcatcaaaaa caaaagagct aaaggcactg
    19081 tacaaaagta ttttaaaatc agattaaaga ggtaaaacaa ctcttggcta gtcacatcat
    19141 atgggccaat tccttttaac aataaagaag aactaccata aatcttatca ccataatggg
    19201 gagcaaaaag atagaccatt ataatattta aaacagcgaa tacaacaact aaagtcataa
    19261 caaaggacac ctgttgccat ttgatttttg ctaatctaaa taagactagc gatcccaatg
    19321 aaacaacaat taaaaatctt gtatcataag tggtcatgca agcaacagaa actaaaataa
    19381 aaaatagtaa cttactggca cctgatagcc tgtatagaaa gccataaccc tcttgataac
    19441 caattaattg tttagccatt cttacctcct tgattatcaa tgtaccattg tgttagtttc
    19501 tgtggagata tccctaatct gtctgctaaa gcaaataaac tcgttttctt taaatgagtc
    19561 tttttcaaaa gctctgtatt tgaaaggatg ttaataggat gatcatctgc aacaatcaga
    19621 ccattgtcga ttacaattgc acgatctgta tactctaaca taagctgcat atcatgtgta
    19681 atcataacaa tggtatgtcc atcacaactt agtttatcaa ggaaagacat catttcagta
    19741 taatgcttca tatcttgacc agctgttggt tcatccaaaa taataacttc tggatttaaa
    19801 atcagtatcg aagcaatcgt aacacgtttt ttctgaccga atgatagagc cgatataggc
    19861 caattcctaa attggtagag cccacaaacc tttaatatat cgtagacacg actttctata
    19921 tcattatcac taaaacctct taaacggaga cccagagcaa cttcatcaaa aaccatcgct
    19981 tgacttatca tctgattggg attttgcaaa acatagccaa ttcgttctgc tctttctttt
    20041 atagagtctt gactgacatc ttcattatta taaattagag tagcattatt agggataaat
    20101 tgacataacg ctttagctaa agtagattta cctgcaccat ttttcccaac gatagctaac
    20161 cgctgacctt tatataaatc aaacgaaaca tcatctaaag tattattctc taaatcatat
    20221 gacaccgaca aatgctgtac tgataataat ttatctgtat gtttatctaa atctaatgct
    20281 cgcattttag gtatcaatag ttcactgaaa tcaaagtcat caatagacgt cattgtattt
    20341 tgtttttcaa agtcatatcc taaatttttg agagcagaaa tatacagtgg ttctcgaata
    20401 ccatattcag ataaaaaatt agtttttaaa agttgatccg gctccccatt ataaataatt
    20461 tgtccatcac taaaaagaac tacacgatct actcgctcat aaagaacatc ttccaatcga
    20521 tgttcaataa taatagtggt cgcatcttgc tcttgatgaa tacggtcaac caatgccatg
    20581 atatcttgtc ccgattgtgg atctagatta gccaaaggct catcaaataa tagaattggg
    20641 ctatcgtcta ctaaaactcc agctaaacta acccgttgtt tttgtcctcc cgacaagtct
    20701 tgtggacgtt tagacaaaag aggagctatt tctaaacgtt ttgcccacat ttcaacgata
    20761 gaggccattt cttcctgtgc tacaacatca ttttctaatg caaaagcaat atcttctgct
    20821 actgtcaatc ctataaattg gccatctgga tcttgtaaaa ccgtagaaac taaatgagac
    20881 ttgtcatata ttgacaagtc aaaagcattt ttatggttaa tcgtaaaaat accactatat
    20941 tggcctttat gagtatttgg gataatacca tttaaacaat gaccaagcgt tgacttcccc
    21001 gaccctgaag gtcctaatat caagaccttt tcacctttag gaatgcttaa gttaatccct
    21061 tttaaagttg gttcagattg aacgtcatat tgaaaggtaa aatccttcca ttcaataaaa
    21121 tctttcatag attactctct tatcctaatc tttggataaa ctaccttttt gtggtcgtga
    21181 tttagcgtat gctagcaaca aaagcgtacc acctactcca atagtaatgg aattcacaag
    21241 gcttgataag aaaccttgtg caaaaacttt gcttgcaggt tcgctataaa agaaaatatc
    21301 accatacggt gcaacgacag accaacctat aaggttagca ataacttgaa ctacattaaa
    21361 tagaagcaaa tccttcttaa tcatgccaga taattgaata gctaattttt tagcaaaaaa
    21421 gcctatcatc aaacctaaaa gaccactaac taaaacccaa gtccaccacg gagaaccata
    21481 ttgaatcgaa tctttcaatg cgtgtccgat aaaaccagta aaaaaaccaa ctcctggtcc
    21541 ataaataact gcaaataatg ctaaaactgc atactgcaat tgaatattag tatttggaat
    21601 tggtgttggt atattaacta gcatacctat aatgataaaa agtgcagctc caattccagt
    21661 cgctacaacc ttttttattg tgtttgtatt cataaaaacc tctctgaaaa acaagcttaa
    21721 acagtcactt acaatctgtt ttattttatc atatttcaag ctgatttgaa accttattga
    21781 caagaagaac tgaaaaacga ttgaaaataa tattaaatac caaaaaagat ctcagataga
    21841 atacctaaga ccttcattag tttatttttt tccaaaaact gatttttcag ctacagccgt
    21901 gaacagaacg tctgtcgatg aatttaaagc tgtctcacac gaatcctgta tcacccctac
    21961 aatgaaacca actcctacga cttgcattgc aacgtcattt gaaataccaa ataagctaca
    22021 tgccaccgga atcaataaaa gtgaaccacc agttacacca gaagctccac atgctgatac
    22081 agcagcaacc acacttaata agaatgctgt tggaaaatca acagtaattc ctaaagtatt
    22141 tactgctgct aaagtcagga tattaattgt aatagctgca ccagccatat tgatagctgc
    22201 accaagcggg atagatactg agtaagtgtc tttatctaat cctaaatcct cacatagtct
    22261 catattaaca ggaatattcg ccgcagaact acgtgtaaag aaagctgtaa tacctgaatc
    22321 ctttaagcat cgtaatacca aagggtaagg atttttacgc atcataacaa aagcaatgat
    22381 tgggttaacg acaagagcta caaataacat cgtacccacc aaaactaata ttaaaagacc
    22441 ataatcacct agtataccaa taccgttttc tgaaatgcta gtgaaaacca accccataat
    22501 accaattggg gccaaattga taatccattt gacaacttgt gaagtagcat cagcaaaagt
    22561 ttgcattaaa tcttttgtac gctgattact gcttcgcatc gccaaaccaa aaataaccgc
    22621 ccaaaataga atactcatat aattggcctc ggtaatggca tgaatgggat tatcaacaat
    22681 tttcaacaaa agagattgaa agacttctga aatccccttg ggaggtaata agtctgtctt
    22741 tgctggcgtt ttcaatatca gtgtcaaagg gaaaatatag ttcacaacaa cagcgattag
    22801 ggccgctgca aaagttgcag tgatatacaa accaattatc gttgacatat tggtcttacc
    22861 gccctcacgg tgttgcgaca aggcactaat aactaaagtg aaaactaaaa gaggggcaat
    22921 agctttcaac cctcctacaa aaagttgacc aagtaaacca atcgcagagg ctttagggac
    22981 taaaataccc aagaataagc caataacgac tccaattgtt atttttttta tcaagcttgt
    23041 ttttctccaa gcttttaaaa atcgtttcat actaaactcc taaaaaattc ttaaagtcta
    23101 tgatatgata aaaaaaaaga ttcgtcaaat acctt
//
LOCUS       DATRNX010000025        22030 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRNX010000025 DATRNX010000000
VERSION     DATRNX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..247
                     /locus_tag="VBC81_000764"
     CDS             <1..247
                     /locus_tag="VBC81_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO6911822.1"
                     /translation="LLTQNGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYS
                     VYLSHAIFGVYQMWITRGKKESPQQITQVLLSLLPQT"
     gene            complement(333..629)
                     /locus_tag="VBC81_000765"
     CDS             complement(333..629)
                     /locus_tag="VBC81_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6911823.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(873..1484)
                     /gene="rpsD"
                     /locus_tag="VBC81_000766"
     CDS             complement(873..1484)
                     /gene="rpsD"
                     /locus_tag="VBC81_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6911824.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1814..2101)
                     /locus_tag="VBC81_000767"
     CDS             complement(1814..2101)
                     /locus_tag="VBC81_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6911825.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            complement(2113..3468)
                     /gene="dnaB"
                     /locus_tag="VBC81_000768"
     CDS             complement(2113..3468)
                     /gene="dnaB"
                     /locus_tag="VBC81_000768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6911826.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            complement(3511..3963)
                     /gene="rplI"
                     /locus_tag="VBC81_000769"
     CDS             complement(3511..3963)
                     /gene="rplI"
                     /locus_tag="VBC81_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6911827.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            complement(3969..5951)
                     /locus_tag="VBC81_000770"
     CDS             complement(3969..5951)
                     /locus_tag="VBC81_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6911828.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            complement(6018..7919)
                     /gene="mnmG"
                     /locus_tag="VBC81_000771"
     CDS             complement(6018..7919)
                     /gene="mnmG"
                     /locus_tag="VBC81_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6911829.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            complement(8089..8700)
                     /locus_tag="VBC81_000772"
     CDS             complement(8089..8700)
                     /locus_tag="VBC81_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6911830.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            complement(8732..9853)
                     /gene="mnmA"
                     /locus_tag="VBC81_000773"
     CDS             complement(8732..9853)
                     /gene="mnmA"
                     /locus_tag="VBC81_000773"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6911831.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10099..10767
                     /gene="sdaAB"
                     /locus_tag="VBC81_000774"
     CDS             10099..10767
                     /gene="sdaAB"
                     /locus_tag="VBC81_000774"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6911832.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            10782..11654
                     /gene="sdaAA"
                     /locus_tag="VBC81_000775"
     CDS             10782..11654
                     /gene="sdaAA"
                     /locus_tag="VBC81_000775"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6911833.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(11789..12493)
                     /locus_tag="VBC81_000776"
     CDS             complement(11789..12493)
                     /locus_tag="VBC81_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6911834.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(12599..13138)
                     /locus_tag="VBC81_000777"
                     /pseudo
     CDS             complement(12599..13138)
                     /locus_tag="VBC81_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(13354..14148)
                     /locus_tag="VBC81_000778"
     CDS             complement(13354..14148)
                     /locus_tag="VBC81_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6911835.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(14141..14983)
                     /locus_tag="VBC81_000779"
     CDS             complement(14141..14983)
                     /locus_tag="VBC81_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6911836.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(14959..15798)
                     /locus_tag="VBC81_000780"
     CDS             complement(14959..15798)
                     /locus_tag="VBC81_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6911837.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(15798..16340)
                     /gene="pgsA"
                     /locus_tag="VBC81_000781"
     CDS             complement(15798..16340)
                     /gene="pgsA"
                     /locus_tag="VBC81_000781"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6911838.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(16463..17746)
                     /locus_tag="VBC81_000782"
     CDS             complement(16463..17746)
                     /locus_tag="VBC81_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6911839.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            complement(17748..18992)
                     /locus_tag="VBC81_000783"
     CDS             complement(17748..18992)
                     /locus_tag="VBC81_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6911840.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            19231..19584
                     /gene="yaaA"
                     /locus_tag="VBC81_000784"
     CDS             19231..19584
                     /gene="yaaA"
                     /locus_tag="VBC81_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6911841.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            19587..20696
                     /gene="recF"
                     /locus_tag="VBC81_000785"
     CDS             19587..20696
                     /gene="recF"
                     /locus_tag="VBC81_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6911842.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(20708..21574)
                     /locus_tag="VBC81_000786"
     CDS             complement(20708..21574)
                     /locus_tag="VBC81_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6911843.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(21630..>22030)
                     /locus_tag="VBC81_000787"
     CDS             complement(21630..>22030)
                     /locus_tag="VBC81_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6911844.1"
                     /translation="YRDNEDIMRIFSFQEQQSCDRIESNVLKILAKLFISNLGLNMRL
                     PQDEINLVVTYLNGVTQYNDFYFKVICYFQDILPEDVILNKISNMTKEQLPYSKSLVN
                     LLIKQVIIALEKDSVAKAIVFADLANKVSQ"
BASE COUNT         7204 a         4238 c         3409 g         7179 t
ORIGIN      
        1 tctcttaacc caaaatggaa caaaagaaat tcagacctac atccttaata aattacaatt
       61 aatgctttct aaagaattac ctgtggtcaa tccagatgca acaaagtctg atatcaaccg
      121 tttgtattat agtgtctatc tcagtcatgc tatttttgga gtctatcaaa tgtggataac
      181 acgaggaaaa aaagaaagcc ctcaacaaat tacacaagtg ctactcagtt tattacctca
      241 aacttaaaac cagccttcct tataggctgg tcatttttag ttaggtttat tttatcctca
      301 ttataggata acaacactaa taaggactga cattataaat gacgatacat ttgagaagac
      361 atatctaaaa attggcctac aagctcgtta tgttctccta taaaatcaaa gagctgatcc
      421 tcactcagta caccgcattt gagattattt tctgtttgtt ctgatagtct aaaccagtct
      481 tcgctaccat aaaattctct taatttcacg taatcttcat agtgcttttg aaaatcatct
      541 atagcttcta ctaatgcatt gattctaggt ttgaagctat catacttctc ttccatttct
      601 tctattttta atctatcatt tagattcata ataaaatctc cttagttata taagattcga
      661 cgagataagt tatcattctc aagtatttgt aacgttaact ctactatatc ctttttacaa
      721 agtaaaaaat tactcattac aaactgacag tgttgatttt tattctaatg ttagtttaaa
      781 atctttttgg ttcattaaat ataatgactc actaaaaagt atatagcaaa aaaagcctat
      841 aaaataggct tttaaagtat gtaatgtaat aattaaagca ttttgttgta gaattcaacg
      901 acaagtgctt cattgatttc tgggttaatt tcatcgcgtt ctggaaggcg agtcaatgaa
      961 ccttcaagtt tttcagcatc aaatgatacg aaagctggac gtcctaaagt agcttcaaca
     1021 gcttcaagga ttgcaggtac tttcattgat ttttcacgaa ctgaaataac ttgtcctgga
     1081 gtaacgcggt atgatgggat atcaacacgt ttaccatcaa caaggatatg accatggtta
     1141 acaaattgac gagcttggcg acgagttgtt gcaagtccta aacgataaac aacgttatca
     1201 agacgacgct ctaaaagaac cataaagttg aaaccaagag ttccttcttt tgctttagta
     1261 gcttgtacga acaagttacg gaattgtttt tcaccaagtc cgtatgaaaa acgaagtttt
     1321 tgtttctcag ctaactgcaa gccatattct gaaagcttgc tacggttatt aggaccgtgt
     1381 tgaccaggta cgtagttacg acgtgccaat tctttacctg tgcctgtaag tgataaacca
     1441 aggcgacgtg attgtttcca tgatggacca gtataacgtg acatctaaat gtcctcctct
     1501 aaaataattg aggaaataac gatttaaaaa atcctgattc gtgcagggga ttttcgccta
     1561 aacagcaaag ggtactagat tagttaacca cctgttgacg agcttcatat ttttctgctg
     1621 ttatttcaca caagctatat tatatcatga aaataatatt gaatcaagtt tttatgtctc
     1681 ttaaacctta aatacaaata gacatctaat aagtatttcc aattataata agaaagattc
     1741 ttaattctaa ccaccaatag agaattagta aattttttta tagaaacaaa aaacctcagt
     1801 cgaggttttc atactattct gctgactcgt catcaaaata acgaattaaa gttttctcag
     1861 ttaaaatatc tgaataagtg taagactcta cataagtatt atcaattgct cctggtacag
     1921 ctgcagtatc cttatactct acgataaaca aagatgggta aacttcaatt aggcgcccaa
     1981 ttttattttt ttccctctta cgaccatttt cgagagtaag ttcgaccatc tgtccctcat
     2041 gtgacttgat atcttctttt atttttttca tttttgcaac atctgcaaat gcatcactca
     2101 tattaacatc tcctattctt caaattgagc tatactagaa aatttattat attccttttg
     2161 aaacattaat tttactgtcc cacgcgcacc agcacggttc ttttctaaaa taacctcaac
     2221 agtattatct tctacaattt cctctgcttc ttcgccctct ctacgataat aatcatctcg
     2281 atataagaaa gcaacaatgt cagcatcttg ttcaatcgat ccagattcac gaatatcaga
     2341 taagaccggt cgtttatcct gcctttgctc aaccccacga gagagctggc ttaatgcgat
     2401 aacaggaacc tttaattctt tagctaaaat cttcaactgc cttgaaattt cagaaacttc
     2461 ttgctgacga ttttctggtc tagttcctga aattaattgg aggtagtcaa tgacgattaa
     2521 acctaaacca tcatcaactt cttgagataa cttcctagag cgtgctctaa tttctgtaat
     2581 tttaatcccg ggcgtatcat caatataaat tggcgcatcc gccaaagctc cttgagcaat
     2641 cgttacatta ttccaatctt gatccgtcaa ctgaccagtt cgtaaactat gagaatcaac
     2701 cattccctct gctgccaaca tacggtcaac taaactctca gcccccattt caagtgaaaa
     2761 aatagctaca ggtctatttt gtttagttcc aacattttga gcaatattta aaacaaaggc
     2821 tgttttacct actgcaggac gagccgctaa gatgattaat tgatctggat gtaaacctgt
     2881 agtaatcctg tctaaatctc taaatccagt tggtaaacct gtgacatctg aggtttgctg
     2941 tgaacgtaat tctaagtttt cgtaattaac ttttaaaaca tcagatatct ttctaaaccc
     3001 gctacgatta ctgtgttcgt taatatcaac taaagccttc tcagcggtgg caataatttc
     3061 atcagaatca tttccttcat aggccatgtt gacagtatct gtcaacttag aaatgatatc
     3121 tcgcaacatg gctttctcag aaactatttt ggcataaaac tccgcattag cacttgttgg
     3181 aacactatta actaattcaa caatatagcc caacccacca atgttttgaa gatcaccttg
     3241 gtcatctaaa atatttctaa cagtagcagc atcaattgca tcatttctat cagctaaggt
     3301 aatcatcgct ctgaagataa ctttgtgtga atatttataa aaatcatctg gacttataaa
     3361 ttctctcacc ataatcagtt tctctggtga gataaagata gatcctagaa cggcctgttc
     3421 tgccaacagg tcttgaggtt gtactcttag ctcagatact tctaccaaac ttcattcctc
     3481 ctgacttgtc aacctttatc atctctcagt ttacgcttct ttgatatcaa gcttaatctg
     3541 tgaagaaacc tgtttatgta attttactgg tacttcaacc ttaccaatcg ctctaatagt
     3601 atgatctaaa tcaatatgac gtttgtcaat tttaatgccg tattgttttt gtaactcttc
     3661 agcaatcttt ttggctgtga tagaaccaaa cgtacgacca tctggaccta ccttttcaat
     3721 aaattgcact cgggtagttt cggattctaa ttgtgttttc agctcctttg cctgtgctaa
     3781 aatttctgct tgtgcttttt cttcggactt ttgttttcct tttaattcac cgatagcttg
     3841 tgtcgttgct tctttagcta gattcttttt taataagaag ttttgagcat aaccagtagg
     3901 tacttcctta acctcacctt tttttccttt ccccttaaca tcttgtaaaa aaataacttt
     3961 catctcaatt aatcccttct attttcgact gtactatttt ctcttaaatc ttcgtcaatt
     4021 atttccagta acatacgtct aacttgcttt acagacttgt cttgtatttg acaagcagca
     4081 aaactaaaat gtccgccacc accaagtttt tccataatac gctggacatt gatattattt
     4141 ctactgcgcg ctgaaatacc aatatttgaa ctgtttttag taataacaaa agtagcctca
     4201 atacctgcca ttgtcaacat ggtgtcagct gctttactag ctataacatg cgagtaaact
     4261 ttatgttctt ctccggtagc aacaatgatg ttatcataaa tacgctcacc ttttaaaatc
     4321 aattcattaa ttaaacgata ttcatcaaaa tctgtagctg atattttttg aatagccata
     4381 ctattactac ctaaaccgcg taaatagctt gccacatcaa aagttctact cgttacatta
     4441 gatgcaaaat ttcttgtatc aagcatgata ccagccatta aaatacttgc ttgactgcgg
     4501 cttaatttat ctttagcttg ttgaaattga atcaattctg taactaattc gctggcacta
     4561 cttgcgccac tttctataaa tgaaagaaca gcattttttg gaaaatcttc atctcttcta
     4621 tgatgatcta ccacaataac atctgcaaac ttgttaaaga attccttaga taaggttaaa
     4681 gctgttttag aatggtctac catgactaat aaagaatttt gagtaattaa ttcaaaagct
     4741 ctctcaacac ttacgagacg tgtttcgccg tcttcttgca agtaatcaat tgctctttca
     4801 acatcagaat tcatgtcatt tgggtcataa actacataac tggcattaac aatattacta
     4861 gcaaaaaatt gcatccctac agatgcacct agtgcatcca tgtccaattt cctgtgtccc
     4921 acaataaaga cactatcaac tactttcaaa cggtcagaaa tagcagtcat catagctctg
     4981 gtacgtgttc gtgaacgttt aatagttgaa acagcaccac caccaaagta aagtgctttt
     5041 ttactactat cattttcacg tactactatt tgatctcctc cgcgaacgag tgctgtatta
     5101 agattttcta aagcaatttg cccaatttga ttatggttac catcaccata agaaattccc
     5161 atacttaatg ttaatgataa atggttttcc tgagcgcgtt ttcgaaactc atttaaaata
     5221 tcaaatttat cttttatcaa tgtatttaaa acgctatagt cggtaaaaat atagtatcga
     5281 tccatattga cacgacgata aaaaatattt ttagactgtg taaaatcaga aataaaacta
     5341 gtcacgaagg cgttaatctt tgacatatca gcctctaaca tagtatccat aatatcatcg
     5401 tagttatcaa tggaaataat accaataact ggacgtagca ttgaactatc atagtttctt
     5461 ctattcccca tgaaattatc gaagaaatag aaaacacccg tctctgtatc caaataagct
     5521 gaatatttgt tatcaccgta ttcaaatgat tgcccagccg ttccattacg tctactagta
     5581 atgatatctt taacagtagc agactgaaaa tgaccattat ccccagtaaa aatcaattct
     5641 gcataagggt taaaccactc aatatcattt gtttcaagat caaattgtat aacaccaact
     5701 ggcatttgtt caagaagact cttaagagaa acttctgttt gatcattaag taattcaatt
     5761 tgttcaactt cagatagttc ataagttatc ttttggtaat ataataaggc aacaacaaaa
     5821 gataaagcaa caaaaatagc caataataga gccgtatagg attgaaataa cctaacacaa
     5881 atagctaata gtccaaataa aatcaggcct attaaaacta aatggacggt tgcaaaacga
     5941 aatcttttca ttaaataacc tcttaagacc taattataac acaaaacgcc cctgataaac
     6001 aggggcgaac actattatca attgatattt ttacgacctt tttgtcttcc ttcaaggtaa
     6061 accattaaaa tactaatatc ggctggatta actcctgaaa tacgactagc ctgacctagc
     6121 gtttctgggt taattttttt gaatttttgt ctagcttctg ttgcgataga atcaatgtcg
     6181 tcccagtcaa tatgaggggg aatacgtttt tcttccattc gtttcatttt tgcaacttga
     6241 tctaaagcct ttttaatata gccttcgtat ttaatttcag tttccaaaag ttcaataacc
     6301 ttactgtcta atggctcatc agcacaacca acaaaatccg ttgctactgc atatgtgatt
     6361 tgtgggcggc gcataaattc tttggcagtt aaagcatctg tcaaaggttt aaagcccaat
     6421 tcttgaatcc gtttatttgt ctcagaaact ggctttaact ttatagagtc taaacgttct
     6481 aattcatttt caaattgcat ttgacgcttt ttaaatatcg cataacgctc ttcatctact
     6541 aggccaatct cataaccgat ttctgttaaa cgcatatctg cattatcatg acgaagaatc
     6601 agacgatatt ctgcgcgact tgtcaacaga cgatatggtt ctagagtccc tttggtaact
     6661 aaatcatcaa tcatgacacc aatgtaagca tcacttcgtt ttaaaataag ttctggcttc
     6721 ccttgaactt taagagcagc gttgatccca gcaaccaacc cttgaccagc agcttcttca
     6781 taacctgaag taccattagt ctgtcctgca gtaaacaaac cagcaattac ttttgtttcg
     6841 agtgttgctc ttaattgatg aggcaataca atatcatact caatagcata gcctgtacgc
     6901 atcatttcag cattttcaag cccttttata gaacgtaata aatcaacttg aacatcctca
     6961 ggaagactcg ttgatagccc ttgaacataa acttcttcag tgtatctacc ttctggttct
     7021 aagaagagtt ggtggcgctc tttatcagca aaacgcacaa ttttatcttc aattgacgga
     7081 caatatctag gaccaactcc cttaacgatt ccagaaaaca taggagcacg gtgtagattt
     7141 tgattgatga tgtcatgact caaagtattc gtataggtca accagcacgg tacctggtca
     7201 gtaatataat cttcatccct agacataaat gaaaaatgat ttggttgctc atccccaggt
     7261 tgtatctctg tcttttcata attaatagag gaggctttta cacgaggagg tgttccagtt
     7321 ttgaaacgac caatttctaa tcctaggtct cttaaattat ctgccaatgt cacagaagct
     7381 aaactattat tagggcccga ggagtactta agatcaccta atataatctc tcctcgaaga
     7441 gcagtacctg ttgttataac aacagatttt gctgaaaatt tctgattagt tgctgtacga
     7501 acccccacaa cctttccatc ttcaacaagt atttcgtcaa tcatagcttg tcttaaggta
     7561 agattttctt gcttttctac agtttgcttc attgtttgtg catagagggc tttatcagct
     7621 tgtgctctta aggcacggac agcaggcccc ttacctgtat taagcatttt catttgtata
     7681 taggttttat caatgttttt acccatttca ccacctaaag cgtcaatctc tctgacaacg
     7741 atacctttag cagagccacc tatagaagga ttacaaggca taaatgctaa catttctaag
     7801 ttgatagtgg ctagaagtgt cttacatccc atacgactag cagctagact agcttccaca
     7861 cctgcatgac ccgctccaac aacgataata tcatagtttt ctgcaaaatt atgtgtcatt
     7921 ttattccttt ccaatttcaa agtctaattc attaaaacac aaaaatagcc aaaactctcg
     7981 aaaattgtac gtcaaaaaga cttacaaatt ccatgagctt tgaccatcat agttattgtt
     8041 aaatttattt tagcatttat cttttaaaag tcaaccagag gggataggct atgttaaatg
     8101 aaaaccaatt tttatgcctg ttattagcat ctctatcccg atagtcatca aaataagtcc
     8161 catcattttg gtgattatag tcattgcatt ttttccaaga aatttagtaa tttcgtttgc
     8221 acttattaaa ataacataca ttatcacaca taatatggca aaagcaagta tagtaatgag
     8281 tttgccagat aaaccaccac ttgataaccc catagcagtt gcaatagtac ccggacctgc
     8341 caaaaggggc attgctaatg gtgaaactgc cacattcatt ggatcatcct tatgctcctc
     8401 taggttttta ttggttggtg aatgtatacc attaatcatt ttataaccta taatgcctac
     8461 caatattcca ccagatattt ttagggcggc tagggtaatc ccaaataagt taaaaagtaa
     8521 gtggccactt aatacaaaga ttacaataat gacaaaagca agtaatactc cttttgctgc
     8581 aatccgacgt gaaatcttct gatcatcatc agctactaaa gccataaaag ctggcaaatt
     8641 agaaatggga ttcataatgg cgaaaaaagc catgaaagct aaaataaatt ttgaaatcat
     8701 acgattacct cacttaataa ttaagtgtca attaaatgta ttgacagatt ttatcatcac
     8761 gataagcctg atcaatcatc ccaccgccta aacattcttg ttcattataa aaaacaacag
     8821 cttgacctgg tgtgattgct cgttgtaaat catcaaacac aacccttgct tgattacctt
     8881 ttacgtaaac tgtaacttta ctgtcaggtt gacgatatct gaacttagct gtacattcta
     8941 gcttaaattc attaggcata tcacgtgtaa agtggatttc agaagctgtc aagctagttg
     9001 acatcaatga gtcatgatag aaaccttgac ctacataaag aatgttttta cttaaatcct
     9061 ttccaactac aaaccaaggt ttattatctc caccatgttg cccaccaata ccaaggcccc
     9121 ctctttgacc aatagtataa tacattaaac cagcatgttc acccatgtca cgaccatcaa
     9181 cagtcatcat acgacctggt tgagccggta aatattgacc caggaaatcc ttgaaatttt
     9241 tttctccaat aaaacaaata ccagtagagt cttttttctt agcggtcgca agtcctgctt
     9301 cttcagctat acggcgaact tctggttttt gtaaatgacc aagtggaaat aacgtttttt
     9361 gcaattgctc ctgagataat tgacttagaa aataagtttg atccttatta ttatctgctc
     9421 ctctaagcat atgaactatt ccgttctcat cacgtgttac ttgtgcataa tgacctgttg
     9481 ctacatagtc agcacctaat gtcatcgcat aatctagaaa tgctttaaat ttaatttcct
     9541 tattacacat aacatctgga tttggcgtac gacctgctcg atattctgct aaaaaatatt
     9601 cgaaaaccct gtcccaatac tctttttcaa agttgacaga gtagtatggt atgccgatct
     9661 gatcagcaac agcagcaaca tctttataat cttcagtagc cgtacaaaca ccaaattcat
     9721 ctgtgtcatc ccaatttttc ataaaaacac caataacatc ataaccttgc tcttttaaaa
     9781 gcagggcagt tactgatgaa tcaacacctc cactcatgcc tacaacgaca cgaatgtttg
     9841 aattatcagt cataagaatt ctcccatcaa aaagttttat ttttaactta ggcttgaagg
     9901 gccaagttgt tcttagatca atcatattga ctaagaaaca ctataaaagt gcttatatat
     9961 tatagcacta tttgttgtca agttgtatgt ttttgttgtg ataaatattt attacattgc
    10021 agttttttta tctttttatg atatttttta gaaataatct gttataatga gaatgtacaa
    10081 aaatcacgag gaaaaactat gaaacattta aaatttcaat cggtcttcga cattattggt
    10141 cctgttatga ttggaccatc aagtagtcat actgcaggag ctgtccgcat tggtaaagtt
    10201 gtccattcta tttttggtga acctagtgaa gtaacctttc atttatacaa ttcttttgct
    10261 aaaacttacc aaggacacgg tactgataaa gcattggttg cagggattct aggaatggat
    10321 acagataatc cagatattaa aaactctctt gaaattgctc atcaaaaagg aattaagatt
    10381 tactgggata tcctaaaaga tagtaattca ccacatccaa atactgcgaa aataactgtt
    10441 aaaaatgggg ataggagcat gagtattaca ggagtgtcaa ttggtggcgg aaacatccaa
    10501 gttactgagt tgaatggatt ctcagtttca ttaacaatga atactcctac tttaattatc
    10561 gttcatcaag atatacctgg catgattgct aaagtaactg atattttatc tgactttaac
    10621 attaatattg ctcaaatgaa tgtaacacgc gaaagtgcag gagaaaaagc tatcatgatt
    10681 atagaagtag attcacgaga ttgtcaacaa gctgtcaaaa agatagaagc tattcctcat
    10741 ttacacaatg tcaacttctt tgattaagag aaagggtaac tatgttttat acaattgaag
    10801 agctggtaga gcaagctaat agccaacata agggtaacat agcagagctc atgatccaaa
    10861 cggaaattga aatgactggt agaagtcgtg aagaaattcg ttatattatg tcccgaaacc
    10921 ttgaggttat gaaagcttct gttattgatg gattgacccc tagtaaatca atcagtggtt
    10981 taacaggcgg tgatgctgtc aagatggatc aatatttaca atcaggaaaa actatttcag
    11041 ataccacaat cctagctgcc gttaggaatg ctatggctgt taatgagtta aatgctaaga
    11101 tgggactggt ctgtgcaaca ccaactgcag gtagtgcagg atgtttacca gctgtgattt
    11161 ctacagccat tgaaaagctt aatttaacag aagaagagca acttaatttt ctatttacag
    11221 ccggcgcatt tggtcttgtc attggtaata atgcctctat ctcaggtgca gaaggaggtt
    11281 gccaagctga agttgggtca gctagtgcta tggctgcggc tgctttagtt atggctgctg
    11341 gaggtactcc tttccaagct agccaagcta tagcatttgt tattaaaaat atgcttggac
    11401 ttatctgtga ccctgttgca ggtttagttg aagtcccttg tgtgaagcgg aatgctcttg
    11461 gatcaagttt tgcacttgtt gctgctgata tggccttggc tggtattgaa tcgcaaattc
    11521 cagtagatga agttattgat gcaatgtatc aagttggatc aagtttaccg actgcttttc
    11581 gtgaaactgc agaaggagga cttgctgcca cgccgacagg aagacgttat agcaaagaaa
    11641 tttttgggga ataaagttta tcaattttct actctttccc tgaaaataaa taaaaacgct
    11701 acagctttac tgtagcgttt ttatttattt gttttagcaa tctgtaatta ttattgacaa
    11761 tagtgtaaag ttaatttata gctattcact agtaacccca agctgataaa ccttgagcac
    11821 gataagcttt aatagctgaa ttaacttgat cctgaactgt agctgttgaa ccccaacctg
    11881 gcatcgtttg gaaaagtcct gaagctcctg aggcattagc aacattagga ttaccatttg
    11941 attcacgggc aataatatgt tcccaagtag actgagggac tcctgttgca gcagccattt
    12001 gtgctgcagc agcagatccg accgcccctg cagtatttcc attgctcaat acttggccac
    12061 ttgtctggtg ttgagcaggt ttgtaagttg tctcagtaac agcataagtt tgttgtgcct
    12121 gactggtagc aggggtattt tctgttacaa ctgcttgttc tacagccgcc tcttcactcg
    12181 cagtaacttg ttgctgagaa ttagcttctg tagattgaga acttgatttt ggggcttcat
    12241 tagatgcctg agaaggtttt ggagcctgtt ttacatcttc tacttttgat ttagatgtcg
    12301 ccttagtcat ttttgatttt ttggctacgc gaactttatc tgcttttgac aaagattcag
    12361 tagtaacttg tgaactttta aagatgaccg ttacagccaa aatacatgaa aaaagggcta
    12421 ctaaagtgat agctccataa gctaaatgtt gtttttttac attcaatttt gataattttc
    12481 ttcttttatt catgttacct atattatact ttatttgtta ccttgcaatt gaatagatgt
    12541 taaaaatatt acaaagagat ttaattttaa gtaaatctct ttgttttatc aacttttatt
    12601 aataccaacc gttactattc caaaatgata gcgctgccga ccaagatccg taacgagaag
    12661 ccacataatt gtccgctact ttttcttgat tttcaggaga taagtcgcca tttaggtaag
    12721 attgagacag ttgatatctt ccataatatt gtccattctg tgcagtataa ctaccatttg
    12781 attcacgacg agctatttct tcttttgcag ctgaatcact tgaactcaaa tttgatgaag
    12841 aattcattga attttcaatt gttggttgag attttgcttc tgcttgacta gctgtagaat
    12901 tatccaattt taaaacatca cctatactga tgacatcagc gttactgata ctattgagag
    12961 acactaacta ttgtaccgta gttttatgat ttttagcaat agctgataag gtatcacctg
    13021 atttcacggt ataagatgct gcgcttactg aaagtggtaa agctaatgaa gcacccacta
    13081 aaagacctgc cactcctaat ttaaatggtt ttgatttttt aaccgaggtt attgacataa
    13141 tatatgctct ctttcattaa ttgatacgcg tactatcata ccttgtaaat attactaatt
    13201 gttttgcctt agatgaaatt tattacaaat aaattgtaaa ttaataaaga aaaggcctcc
    13261 aaaaaacaat ttttagtttt ctttaagtaa agttgttgag cttttcacat tatataaatg
    13321 aaaaggctaa gaccataaac tgaccttaac cttttaattt tttaatagaa acaaactcaa
    13381 acccaataat aacatcagta atatactaaa agtatcccga acagtccact tcaataaacg
    13441 gtacttacta cgattggctc caccttgata acctcttgct tccatagcaa ttgccaacgc
    13501 atctgctctt ttaaaactag aagcaaataa tggaatcaaa atagggataa tggacttaac
    13561 cttatgaatt aggtttccct ctccaaaatc aactccacga gctctttgag cattcatgat
    13621 acgtgttgta tcatccatta aagtaggaac gaaacgtaag ctaagggata acatcaaacc
    13681 aatctcgtgt gctggtactc tcagtacttc caatggtttt aacaaagatt caacagcatc
    13741 tgctaaactt aacggagtag tagttaaggt aagtaatgtc gaaaagaaga taattaaaac
    13801 aaatctcata aaaataagtg ctgcttgttg taacccaagg ctagtgatgc taaaaatcca
    13861 aaacgagaag ataacctgcc cgccttgtgc aaacagcatc tggaacaggg ttgtaaataa
    13921 aataatacca accatcggtt taataccatt gaaaaaatag gaaaatttta tctgagataa
    13981 acccacaata accaacgtaa aaatgaatac gatcacattt gtcactacat tatttgccca
    14041 aaaaacaata atgataaaaa gcaacatggc tagtaactta cttcgaggat ctaatttatg
    14101 aatgagagaa ttacctggta tgtaacgtcc caaaatcaat ttatccatgc ttaatagcct
    14161 ccacaaattc gttaatagta attggtaaac taggtaaatt taatccctta tgagatagtc
    14221 tttgagcaaa cttggtgatt ttgggaactc ctaattgttt actttctaaa agttctactt
    14281 cttgaaaaat ctgttttggt tgtcctgata aggttacttt ccctgcttct aaaacataca
    14341 catagtcagc ataatccgct acatcgtcca ttaagtgagt cactaagacg atagtcattc
    14401 cttttttatg aagattttta aaaagagtca ttaattcttt tcttccctta ggatcaagtc
    14461 cagctgttgg ctcatccagt actagtactt tgggttccat cgctaaaata ccagctatag
    14521 caacccgcct catctgccct ccagaaagtt caaatggatt tttatcgaat aaatcctcac
    14581 tgataccaac taaccttaat ttttcttcag ccagcctttc agcttcaatc tgagaaatac
    14641 caaaattttg tggtccaaaa gcaacatcct ttaaaactgt ctcttcaaaa agctgacttt
    14701 ctggaaattg aaaaactaaa ccaacttttt gccttataaa tttgatttct ttgttcttgt
    14761 cccctgcttt aatagaaaaa tcatcgacaa ttacctcacc ttttgtagga atatgtaaac
    14821 cattcaaaag ttgcataata gttgattttc cagaacctgt gtgcccaatg aacgcggtat
    14881 aggaaacatc ttcaattttc agattgacgt caaaaagggc acgcccttca aaaggagtgc
    14941 cggcttgata ggtataactt acatttttaa attcaattcc cataattgtt cttctaattc
    15001 cttctctgta agatactctg tccctatatc aagaccatta gcagccagtt ctgccattaa
    15061 cctacttgta aagggaacat ctaaacctaa actaatcaaa cgatttccac gaccaaataa
    15121 cgcttttgga gtggatgtcg actcaacttt accatttttc ataacgatga cacgatcact
    15181 caatgcaact tcatctaagt cgtgtgtaat tgaaataaca gtaagattat atttttgacg
    15241 aatagctcgt atcgttctta tcagttccaa tcgtccctca ggatcaagca tactagtcgc
    15301 ctcatccaag atgataactt gtggacgcat agctacagct cctgcaatgg ctacacgctg
    15361 cttttgtccc ccagataaac gtgcaggctc cctcatttta aattcagaca tgcctaccaa
    15421 atcaagtgct tggtccactc tttctttcat gtctttcaaa ggaatacctt tattttccaa
    15481 accaaaggcg acgtcatctt caacagttgc ccctacaaat tggttatcag gattttgaaa
    15541 taccatgcca atcttgtgac gtaactccca gacattatct tcggttaact cttgtccatc
    15601 aatgataatc tggcctgact ccgcctctaa aagcccatct attaaacgaa cagtagtgga
    15661 tttcccagaa ccattatgac cgataataga tagccattct ccttgtttca catgaaacga
    15721 tacattctcc aattgataat gtgtttgatt actatcatat ttaaaaaaga gattattaac
    15781 agtaataata tttgtcatta tttaaaagta tccttaaata agaagcctgc acctttaaaa
    15841 tagtcgtagc ctgaatacaa ggtaaagaat aaagcaatgt ataacatgat gttaccaagt
    15901 gtcatccaat gtactaacaa aaaaataact gcaaacattt gagtagccgt ctttattttc
    15961 cctggcatag ctgcagcaag tactgttcca cctgtttcaa ctaaaagtaa acgcaaacct
    16021 gtaactgcta gctctctaca aatgataata gctgaaaccc aagccggtgc taagtctagt
    16081 ccaactaaca tgataaaagc actcataact agcattttat cagctaatgg atctgcaaat
    16141 ttcccaaaat ttgttactac ttgccattta cgtgctagat aaccgtctag gtaatcggtc
    16201 aagcttgcaa tagcaaatac aatagctgca acaatatgcc aagttgtact tgttgttact
    16261 gaagttaata ctataaataa cggaatcatt aaaattctga ctacagttaa tagattcgga
    16321 atattttctt tcttcaccat aaattctcct tttggaaaca tagtattcct atattatgtt
    16381 atttcaaaac tagtaaaaga cttttgacat accaacttta tagatgtcta agcttcctta
    16441 ttatatcaaa atttcagatt atttacgaag attttggaaa aataacgaag tcagatgtgt
    16501 cagcattatc taagaaatcc ttaccaatcg taatgacatc ttccaagtct aattcttcaa
    16561 cgatagatgg caaatctaaa tacatcgttt ttccatcata taaataggtg acgaattgca
    16621 ttgcgagatt ttcaatagaa tctaagctcc ttaaaaaatc gccatagagc tctctcttaa
    16681 cgttttgtaa gtggctttcg gtaagatcag atgattgcaa agcatttttt agcaataacc
    16741 tcaactgagt tgaaaaagca attggctctg tcgtatctaa agaaataata acgcactcaa
    16801 aatctggatg aacttcaatt tctatatcaa aagagtcatc aatttgacca gattcgtacc
    16861 agtcttgatt aattgtagaa gtccatccta aaagcattgc aaaaaatagc tgtaatccga
    16921 ttttctcttt taggtaagaa ccatgtctca tatgacttga ttttcgataa ccaatagcta
    16981 atttaggttt tgtcactgtc atatggcaag agttgttttt tataacaggt tcaagactaa
    17041 tagaatctcg gtcaatattc tgtgagtaat ttgttaaatg agaatttttt tgaaggtaag
    17101 ttataatttc ttgtggacta aattgtccaa caagaactaa attcatatta gacaactgat
    17161 aaaagtcttt gtgattttct tgtaaatcgg ttaaggttat ttgagagatt gattggtaat
    17221 ctccagcgat atcaaatgct aatggagagt tggggtaaag gttcgataac actccttgat
    17281 aaagcctata ttctgggtca tcttgataca tttcaatttc ttgtttgata atgtcttttt
    17341 ctttagtaat tgactcttct gtaaagtttg ttgtcaatac aaagtcaagt agtatatcta
    17401 agcagtttgt aatgtgactt atagttgaaa aataaaaact agttttatca aaagttgtaa
    17461 aggcattact ctcggcgcca tatttagtaa attgagttgc agcgtcttgt cctttatcca
    17521 gttcaaataa tttatgttct aagaagtgag caattcctgc cggataatgt tctacacaac
    17581 cattcctcgt atatcttgta tggagtgatc caaaatttgc tgtcaatact ccaacagttt
    17641 ccttaaatga tggttttgga ataagatata catttagccc gctctctaat gttaacttat
    17701 atacttcctc ctgaaggttc tgataagtta ttttattaac tgtcatccta gtttccttcc
    17761 aaaaaataaa aagcttgtaa ttttaatttt gttgctacgc tgacaatatc tgattttgtt
    17821 acatcgtcca ctttgtcaat gaatagtgct gaagtatgaa actgatctaa ataatgttca
    17881 tgataaatgt ggtcaactat gaccttagga ttatcagaag ctaaaacata gttcattttc
    17941 aatatatctt ttgtttgttt caataatgta gaagagaacc tgcccatctt aatattattg
    18001 aattgcttat taataagctt caaaaaacgc tcacgattct ctttatcaat acctgcatat
    18061 atcgtaaaga gacctgtaaa gctgtcaaat tgactaccta tagtataggc taaaccttgc
    18121 ttttctcgaa tttctgtaaa caacaaggaa tgggcaaaag caccaaataa accgttaaat
    18181 actattaaag caaaataatc ttcatcttta taagttattg gaagatgata agccaattgc
    18241 ataatagatt gattaacgtc cttatcttct acttgacttc tcgtgatatt ttcataagtc
    18301 tgcgtataat caaaagctaa tactttgtga cgtggctcaa atgccatacg attaaatgcc
    18361 tgaatcattc gatagtcatc aaaatctcct acgacaaaaa tatctaattg atcttcctta
    18421 agcatcttct gaaattcttg ataagctgta aatgaatttt ctgattccac taaagatgct
    18481 gtaccatact taggtaaacg aagtgtctta ttattgtaga ataaagattt tagtgctaag
    18541 tcactgctat aaaaattatc ttcaatatcg gcctctaaat attgaataag gtttttcttc
    18601 tcagtatcaa aaatagatgt ttgatactgt tctaaagaaa ttaaaggaga aaacagcata
    18661 tcctccaaga atgtaataat ttgttcaacg atattttctt gttctaaagt aaaggtattt
    18721 ttaacaaata caatatcaat atcaacaatg tgaacaagac ccttagtaga aatcttagtt
    18781 gataaactag caccatataa ggaagctaac ttttctcgaa attcttgtac cttagggtac
    18841 ttagcatttg cagtaactaa catttgagca acgagcgatc tacgtgctac tgttttatta
    18901 ttaaaatcac ctgaaaatcg aaaagtaaga tgattagtct taaattgttg gtttttaatt
    18961 aaatgtagag aaacgccttc aactattttc attcaatatc ctttgtgttc tgtattgtta
    19021 gctaagatta tatcattttt aaaggcgtta aaaaagatta aacatccaag atatgattaa
    19081 ttctaaattt caatcttctt caaaattgac aaagtcgtca accaatattt actcccattt
    19141 tttatacctt gtctccatat cataagttca actcaaaaaa taattttatg ctataatgat
    19201 gtgatatcta aaattggaga ttaaaaagtc atggattata aattatttga tgaatatatt
    19261 actttacaat cacttctaaa agagattggt atcattcaaa gtggcggagc cattaaaaaa
    19321 tttttagcgg ataatcaagt attgtttaac ggtgaccttg aaaataggcg tggaaagaaa
    19381 ctccgcctgg gggatattat tactattcct gatcaaaata tcgagatcat tataaggaag
    19441 ccatcagatc aagaaattga agaacgtaat gtagaaatag ctgaaaaaca gcgtgtttca
    19501 gcaatcgtta aagaaatgaa taaaaacacc aacaaagata aatcaaaaac tagcaaaaaa
    19561 ccagttcgct ttccagggac ataaaaatgt ggataaaaaa tatttcttta aaacattatc
    19621 gtaattatga agaagcacaa gtagattttt cacccaacct caatattttc attggccgca
    19681 atgctcaagg aaagacaaat tttttagaag caatctactt cttggctttg actcgaagtc
    19741 accgtacccg ttctgataaa gaacttgtac attttaaaca ccatgatgta caaattacag
    19801 gtgaggtaat ccgcaaaagt ggccatctta acttagatat tcaattatct gaaaaaggaa
    19861 gaatcactaa agttaaccat cttaaacagg caaaattatc tgattatatt ggggctatga
    19921 ctgtcgtact ttttgcccct gaagatcttc aattagttaa aggtgcacct agtctaagac
    19981 gaaaattttt agatatagat attggacaaa ttaagtcaac gtacttagct gaactttcaa
    20041 attacaatca tgtattaaaa caaagaaaca cttatctaaa aactactaat aacgtcgaca
    20101 aaactttttt aacagtcctt gatgaacaat tagctgatta tggaagtcgt gtcattgaac
    20161 accgttttga ttttatccaa gccctaaatg atgaagcgga caaacatcac tacattatca
    20221 gtactgagtt agaacattta tctattcact acaaatcgtc aatagagttt acagataagt
    20281 caagtattag ggaacatttt ttaaaccaat tatctaaaag ccactcaaga gacatcttta
    20341 agaaaaacac aagcatcgga cctcatcgtg atgatattac tttctttatt aatgatatca
    20401 atgctacttt tggtagccaa gggcaacaaa gaagccttat tttatcattg aaattagcag
    20461 aaattgagct aataaaaacc gttaccaacg attatccgat tctcttactt gacgacgtca
    20521 tgagtgaact tgacaaccat cgtcaactaa aactcttaga gggaatcaaa gagaatgttc
    20581 aaacatttat tactacaaca agtttagaac atttaagtgc tttacctgat caactcaaaa
    20641 tctttaacgt tagtgatggt actattagta ttaatgaaaa aaaagcaaca gattaatctg
    20701 ttgcttttta ataagattta acaataccaa gtaatatagc acccataaca aaacaaagga
    20761 tacccatgac aacccatttc tgctctttct tcgtttttgt ctcacctaaa aataaaatac
    20821 caccaataat agagataatt actccaagtt gagaaaaact aaaagcaatt gctagccctg
    20881 ctttagctgc tgccaataac atgaagacat taccaacgcc ccacatgaga cctgtaatca
    20941 tatttttaac aacaacagcc tcaaagttaa cacgaaactt catgaaacaa attgcaccta
    21001 gacacattcc aacagccatg ggtaaaatga cggccatagc gtcgaacttc ataatgttgt
    21061 taaataaaac tgcgtacgag atgtaaccta cagttgaata agcaattgta gcaaatcctt
    21121 ttgaaaactc ttgatgtgtt gccaaagctt gttctgaaac atcacgttta cttgagaaat
    21181 agaagccgat aactaataat gtcaacgctg tcaatcctaa aataaattgg attggcttag
    21241 tccattcatg aaagactaaa gcaccaacta ggcttccacc tactaattgt gcaccacttg
    21301 acagtggatt agcaacagag actcccatat atttcattgc ttgaaattga ccattttggc
    21361 cgactgacca taggatacca cctaagatac caaaaatcca caatgaggca gtcatctctg
    21421 gctgtttaaa taaccatacg ataatcgcaa atagcaatgc tcctaaagtc attccaaatg
    21481 tttgttgatt tggacgccct ccaattttat tactaacaaa tccaatactt ccccacgcaa
    21541 acatgggaat caatgcaata agtaaacctt ccaattcttt ctccttacaa tggaatatta
    21601 agtattacct attttcaatc ctagagaatc tattgactaa ctttattagc aagatcagca
    21661 aatactatag ctttagcaac actatctttt tccaaagcta taatgacttg ttttatcaat
    21721 aaattgacca gtgatttaga ataaggtaac tgttctttag tcatattact aatcttattt
    21781 aaaataacat cttcaggtaa aatatcttga aaatagcaga tgactttgaa ataaaaatca
    21841 ttgtactgtg tcacaccatt cagataagta actaccaggt taatctcatc ttgaggcagc
    21901 ctcatattta accctaaatt tgaaataaaa agtttagcca atattttgag aacattgctt
    21961 tctattctat cacaggattg ttgttcttgg aaactgaaaa ttctcatgat atcttcatta
    22021 tctcgataag
//
LOCUS       DATRNX010000026        21125 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRNX010000026 DATRNX010000000
VERSION     DATRNX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21125
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..632)
                     /locus_tag="VBC81_000788"
     CDS             complement(<1..632)
                     /locus_tag="VBC81_000788"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6911845.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVG"
     gene            complement(699..1370)
                     /gene="deoC"
                     /locus_tag="VBC81_000789"
     CDS             complement(699..1370)
                     /gene="deoC"
                     /locus_tag="VBC81_000789"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6911846.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(1400..2602)
                     /locus_tag="VBC81_000790"
     CDS             complement(1400..2602)
                     /locus_tag="VBC81_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6911847.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(2623..3402)
                     /gene="udp"
                     /locus_tag="VBC81_000791"
     CDS             complement(2623..3402)
                     /gene="udp"
                     /locus_tag="VBC81_000791"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6911848.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            3561..4298
                     /locus_tag="VBC81_000792"
     CDS             3561..4298
                     /locus_tag="VBC81_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6911849.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            4311..4675
                     /locus_tag="VBC81_000793"
                     /pseudo
     CDS             4311..4675
                     /locus_tag="VBC81_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4707..6329)
                     /gene="groL"
                     /locus_tag="VBC81_000794"
     CDS             complement(4707..6329)
                     /gene="groL"
                     /locus_tag="VBC81_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6911850.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(6425..6709)
                     /gene="groES"
                     /locus_tag="VBC81_000795"
     CDS             complement(6425..6709)
                     /gene="groES"
                     /locus_tag="VBC81_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6911851.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(6884..7687)
                     /locus_tag="VBC81_000796"
     CDS             complement(6884..7687)
                     /locus_tag="VBC81_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6911852.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(7692..9566)
                     /locus_tag="VBC81_000797"
                     /pseudo
     CDS             complement(7692..9566)
                     /locus_tag="VBC81_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(10031..10828)
                     /locus_tag="VBC81_000798"
     CDS             complement(10031..10828)
                     /locus_tag="VBC81_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6911853.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            11014..11874
                     /locus_tag="VBC81_000799"
     CDS             11014..11874
                     /locus_tag="VBC81_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6911854.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            11917..12648
                     /gene="yaaA"
                     /locus_tag="VBC81_000800"
     CDS             11917..12648
                     /gene="yaaA"
                     /locus_tag="VBC81_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6911855.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(13005..13622)
                     /gene="nrdG"
                     /locus_tag="VBC81_000801"
     CDS             complement(13005..13622)
                     /gene="nrdG"
                     /locus_tag="VBC81_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6911856.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(13695..14186)
                     /locus_tag="VBC81_000802"
     CDS             complement(13695..14186)
                     /locus_tag="VBC81_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6911857.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(14195..15127)
                     /locus_tag="VBC81_000803"
     CDS             complement(14195..15127)
                     /locus_tag="VBC81_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6911858.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(15140..15283)
                     /locus_tag="VBC81_000804"
     CDS             complement(15140..15283)
                     /locus_tag="VBC81_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911859.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(15358..17556)
                     /gene="nrdD"
                     /locus_tag="VBC81_000805"
     CDS             complement(15358..17556)
                     /gene="nrdD"
                     /locus_tag="VBC81_000805"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6911860.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(17655..19196)
                     /locus_tag="VBC81_000806"
     CDS             complement(17655..19196)
                     /locus_tag="VBC81_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911861.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(19447..19764)
                     /locus_tag="VBC81_000807"
     CDS             complement(19447..19764)
                     /locus_tag="VBC81_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6911862.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(19790..20209)
                     /gene="ruvX"
                     /locus_tag="VBC81_000808"
     CDS             complement(19790..20209)
                     /gene="ruvX"
                     /locus_tag="VBC81_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6911863.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(20209..20475)
                     /locus_tag="VBC81_000809"
     CDS             complement(20209..20475)
                     /locus_tag="VBC81_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6911864.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(20677..21075)
                     /gene="spx"
                     /locus_tag="VBC81_000810"
     CDS             complement(20677..21075)
                     /gene="spx"
                     /locus_tag="VBC81_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6911865.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         6766 a         4300 c         3215 g         6844 t
ORIGIN      
        1 tcacctacat atggacgagc gataatacga ccaaggagag ctggacgctc cattgtgata
       61 ctacgagcat attcacaaat acggtaaagt tcttctagag ggatgatatc ctcatgagct
      121 gcaatttgta ggactgggtc agctgaggta tagatgatga gctcaccagt ttccatttgg
      181 cgtggtccga agtcatcaat aacagctgtc cctgaataag gtttattcgc ttcacgaata
      241 actttacgtc ctgaaaaatc ctcaatcttt gtgatgatat cttctggaaa accattccag
      301 aaagtatcaa atggctctgt aatatttaag cccatgatct cccaatgccc tgtcattgta
      361 tctttaccaa gtgatacttc ttgtaacttc gtcgcgtagc cacttggatt ttcttctgca
      421 ggaacggttt taagcgcttg gggacgagga atattaccaa gtccaatttt agccatattt
      481 ggcaccgcaa gtccaactgt ttttgaaatg tggccaagcg tgtctgaagc accatcagga
      541 actcctgcat tgacaaaatc attggcatct ggtgccgctc caatacccac tgaatctaag
      601 acaactaagt gaatgcgatc aaattgtgac ataacttctc ctttatcaat tgaaaatagg
      661 aagtcagacc agcatccttc ctatttcctt tatattattt aataagtgcc ttcttcaact
      721 ttttggttct taacaatctt gatgatacgg ctagtaccca agcgtgaagc tcctaaagca
      781 ataaattttt cagcatcttc caatgatgag attccacctg ctgccttaat tttaacacct
      841 tcgtcgacat attttgccat cacttcaaca tcttcaaatg tagcacctgc tgtcgaaaaa
      901 ccagtagagg ttttaataaa gtctgcacct gaacgtgtga caactccaca aagttcgata
      961 agttcttctt tagttaattg acatgtctca acgataactt ttaagatatg gtcttgacat
     1021 gcagctttga tttgacgaat ttcttcttca acagtatcaa aatccccatt tttaacgtct
     1081 gtcaaattaa tgaccatgtc aatttcatca gcaccatttt taatagcatc ttgacattca
     1141 aaaaccttcg ctgcagttgt actatagcca tttgggaacc caataacagt acaaatagct
     1201 aatttacctg aaacgtactc tgctgctttt ttgacgtaag aggctggaat acatgctgaa
     1261 gctgtttcat aagccattgc atcatctaaa attgtttgga tttctggcca tgttgctgtt
     1321 gttgctagca aagtatggtc taccgttttt aaaatatctt ttacttccac gttactatcc
     1381 ttctttcttt tgtcaacttt tagaatagag ttacaatacc gacgataaag gcgctaagca
     1441 tacttacagc aataccacca atcattgcac ggaaaacaag tcgagctagg gtgccacgtt
     1501 tctctggaca aagaacagca atacctgaaa cacaaatacc taaacttgat aaattagcaa
     1561 aaccacagag tgaaatagtt gctaccaatg ctgtacgata atctaaagat ttgattaggt
     1621 gacccaattg ttggaacgaa acaaactcat ttaaaatcaa cttacttcca agaaggtttc
     1681 cttctagaag aatgtttttg tggtcaaatc ccataagaaa accaaatgga gcaaaaacat
     1741 atgagaagat ttgttctaag cgaattccca atccacttaa catcatatta atcaaagaaa
     1801 ctaaaccaac aaaggcaatc aaactagcac caattgagaa agccatttgt gcacctgtgc
     1861 ttgcaccctc agcgattgca tcaatcacat tggcgttatt acctttatta tccatcttaa
     1921 tgtcatcaat tttttgaaca ggttctgttt gaggaaataa gattttagca atgagaatac
     1981 tgccaatagg aaccattgtt gaagcaatca agagatattc cattggaatg cctaatgcaa
     2041 tgtagccacc aagaatagaa actgacatac ttcccatacc tgataccaac acaaccatta
     2101 tctcactatc agtcatacga cctaggtatt tgctaaccaa aattggactg tctgtttgac
     2161 caagaaacat attagctacg gcaacaaaac tctcaacctc tgaggatttc ataattttac
     2221 caacaccctt acctatccat tttactacaa atccaaggat tcctacataa taaagtagac
     2281 taactagggc agataagaaa acaatattac caagcgtttg aatggcgaaa ataaaaccag
     2341 tttttgttcc actatctgct aatgaaccaa acacaaaatt taaaccagct tgaccacagt
     2401 tgattacttt agtaactcca gttgaaacaa cactaacaac ttgttggcct agtgggatac
     2461 gtactaagat tagcgcaata atgaattgaa cgataagagc ttttccaatt aaacttagag
     2521 aaacactctt acgattgaaa gaaattgcat acacaattcc taataccaat aaaataccaa
     2581 taatactata aataaattgc atgatgactt ccaaaacctc gcttattgtg atttatcatt
     2641 ttcaataagt gtgcggagtg cttcaacagc aacttgaata gctgcttctg tgtcatgagc
     2701 cattgggtta tccataccca aagcgttgcg ttcttgatta ccaacaacaa ggaaatctga
     2761 tccacaacga actcctagat gacttgctgc tacaaaaaga gctgcagatt ccatttcaga
     2821 cgcttttgtt cccagacgtt tccatgcttc ccatttattt aataattcat aactaactgg
     2881 catacgttct ggctcatgtt gtccataaaa tgcatcttta cattgaacta ctcctgcgtg
     2941 acttgtgtaa ccaagctttt tagctgcatt aactagagca ttcgttacct ccaagtcagc
     3001 tacagcagga aattcaatcg gagcatattc tttacttgta ccttccatac ggatagctcc
     3061 agttgcaata acgatgtctc cgcctttaac atcaaggtca ataccaccac atgttccaac
     3121 acggataaag gtatcagcgc cacagagttt caattcttcc atcgcaatag acgctgatgg
     3181 accaccaata ccagttgaag taacactgac tttttcacca tttaatgtac ctgtataagt
     3241 aacatattca cggctgtctg ctacaagaac tgcattatca aaatgttctg caatttttgc
     3301 acaacgtttt ggatcacctg gcatgataac ataacgacct acatcacctg gacgaatctg
     3361 taggtgatat tgcaatccga cttcacctga ataattctgc atgataaaga cctccttaat
     3421 gtcttgtcta ttttttttac acttgtataa taacatacaa gacaaccttt gtaaacgctt
     3481 ttatcgaaag aaaacgtttt attttttaac ttgatttttc taattttttc aattaaacta
     3541 acttgtatac ggaggttaca atgcctaaaa atgaactaaa taataaacta aataaactaa
     3601 agcatgtgca agtctacaat aagattttta acatgattca agacggtact tattctcctg
     3661 gaatgcaatt gccttctgag cccgaattag cagggcaact taatgttagt cgagctactt
     3721 tacgaaagtc attagcacta ttacaagaag accatttggt taaaaatatc agaggaaaag
     3781 gtaatttcat tagagaaaat tctagcaact tgtctgaaaa cggttacgaa aatagacaac
     3841 accccattaa aacttgtcta acttccaaaa ttacagaagt ggaattagaa tttcgtgtag
     3901 aggttcctgc agaagctatc acagcttcac ttaaacaaga aacccctgta gttgttattg
     3961 ctgatcgttg gtaccacacc gatgatggac cattagctta cacactttct tttatcccta
     4021 tcgaacttat ttcagatgct gagatttctt tacatgatac taaacagttg ttaaacttca
     4081 ttgaagaagg tatctatcac gaaggcatat caagccactc acaaagccat ttgggatatg
     4141 cgacatctgg caatttttca gcaactaaat acaccctatc cgatcatgga caattcatat
     4201 taatacaaga aacaattttt aaccaagaga aaatactaat gtgtaataag cattacgttc
     4261 ccattgagca ctttgaattg tccattacct ctcattaagg aggcaagcct atgcaaaata
     4321 gactattaaa agttgctttt ttcataactt ttaccgaatt aattatgggc ctaatagttg
     4381 gcgcttattt taagttaagt tattttctag cccctccaaa ggaaccgtca atgggtgctg
     4441 ggtacggaat gctcttttct atgttattta tacttattag ttacttaatt ctattagcta
     4501 taattcaatt cttagcttat caatctcaat ctaagataat agcaatcatt attttgctac
     4561 ttttttcctc ccactactgg caaatattag atggcctatc cttatgatct tcttaccgca
     4621 agtggtagtg actatcatat ctctattatc acaacataaa actccccttt gttaaagggg
     4681 agttttatgt tgtgataagt tagtgattag aagccaccca tcatagatgg atccattgca
     4741 ggagctgtag gagcttctgg ttcaggttta tttgctacta ctgcttctgt agtcaagata
     4801 agacttgcta cagatgccgc attttgaagt gcagaacgtg ttactttgac aggatcaatg
     4861 atacctgtgg taaccatatc tacccattct ccattagccg cattaaatcc tgtaccaatt
     4921 tcagactgtt ttaaacgttc aataataact gaaccttcat atccagcatt gtaagcaatt
     4981 tgacgaacag gctcttcgag agcacgaaga acaatattac gtccagtctc ctcatcacca
     5041 ttaagtttca gtgccgctac tttttcaata acgttcacaa gagccgtacc tccacctgaa
     5101 acaatacctt cttcaactgc agcacgcgtt gcatttaacg catcttcgat acgaagtttc
     5161 atctctttta attctgtttc agtcgccgca ccaactttaa ttactgctac accaccggct
     5221 aacttagcaa gtcgttcttg taatttttca cgatcaaaat cagaagttgt agcctccatc
     5281 tgtgacttaa tgatggctac gcgattagca attgctgatg agtcaccggc accttcgaca
     5341 ataacagtag aatctttatc tactgttact ttagcagact gtcctaaaac ttgcatagta
     5401 gcatctttta agtctaaacc aaggtcttca gtaacgacag ttcctcctgt taggatagca
     5461 atatcttcca gcatggcttt acgacgatca ccaaatccag gcgctttaac agctacgaca
     5521 ttgaaagttc cacgaatttt gttaagaaca agcgttggga gagcttctcc atcaacatca
     5581 tcagcgatga ttagcaacgg acgatttgtt ttaagaacct cttctaataa tggtaaaatt
     5641 tcttggatat ttgaaatttt cttatctgta ataaggatat acggattctc aagttcagag
     5701 accattttct cgttatcagt taccatatac tgtgacaagt acccacggtc aaactgcatt
     5761 ccttccacaa cttcaagctc tgtttccata cctcgcgatt cttcaatagt gataacacca
     5821 tcattaccca cgcgctccat agcttcagaa atgtattccc caactttttc agaacgtgaa
     5881 gacacagccg caacttgagc aatagcttct ttgcctgaaa ctggttgtgc aatctctttt
     5941 agctcttcaa ctgctgctga aacagctgtt tcaataccac gacgaatgcc aatcggattt
     6001 gcccctgcag ttacattttt aagaccttcc cgtacaatag cttgggtcaa aacagtagca
     6061 gttgtagtgc catcccctgc aatatcatta gtttttgaag ccacttctga cacaagttta
     6121 gctcccatat tttcaaagtg atcttctagc tcaatttctt tagcaattgt cacaccatca
     6181 tttgtaatta aaggagaacc aaatgctttt tcaagaacaa cattacggcc tttaggacct
     6241 aatgttactt tgactgtatc agctaaaata tcaacaccac gcaccattgc tgagcgggca
     6301 tctgctgaaa atttaatatc ttttgccata atttaatacc caggcctaaa atttaacaaa
     6361 gtctactttc tttacaaagc aacttttctc taataggcct atatcctttc ttgcttaata
     6421 aaacctattt aacgacagca acaatgtcac tttcgcgaac aactgtcact ttttcgttac
     6481 cgtctttaac ttccaaacca gcaccatttt cgactaaaac agtatcaccc tctgcaacgc
     6541 taggtgcaac taattcccct gttaaggtac gaataccgtc acccacagca agaactttgg
     6601 ctgtctttgt cgtatcatgg cttgctcctg caagaacaaa accaccgaca gatttctctt
     6661 cagtttcaac aaacgaaatg atcacacgat cacctaatgg ttttaacata ataaacgaac
     6721 ctccatacga gatatatttt gattttttag cactcctata aagagagtgc taactacaaa
     6781 aactattcta tcacttggtc agaattagtc aagagaaaag gcacgtttca aacaagaaaa
     6841 atttccctag ttatcctgtc cattcactag agaaatttat attttaacct aagacaaggg
     6901 catcatctat caactgttga cctgagtttt tatggaaaag ttccattagt tctgcaactg
     6961 ttaagttcct ctttgcttct cctttaacat caacaacaat tttcccgtgg tagagcatca
     7021 cgagacgatt accataagca atggcatgct ccatattgtg tgtaatcatt aaggctgtca
     7081 atcgttgttc ttctatcact ttctgtgtta attccataac catatcgcta gttttgggat
     7141 ctagagccgc tgtatgttca tccagtaata ataatttggg acgaacaaga gttgccatag
     7201 ctaacgtgag ggcttgacgc tgaccgccag ataaaaatgc agcatcagtc ttcattcgat
     7261 tttctaagcc tagacctaat tgtgatagag actttttaaa tagttgacgg tcatcgtcag
     7321 tcactgactg tctaaaaatg tgacgcttat tgcctctttt atgtgcaatt gccatatttt
     7381 cttcaatcgt caaatttgtt gcagttccca ttctaggatc ctgaaagact cgactaatac
     7441 ccttagatcg tttttctaca gagtctttag taattgattg gttgtctaat gtgattgtcc
     7501 cctgatcaat agggatcaga ccagcgatac agttaaggag ggttgatttt cccgcaccgt
     7561 tacctccaat tactgaaata aaatcaccgt cttcaatcgt taaatctaaa ccacgtaaaa
     7621 catggttctc attgacagtt cctttttcaa aagttttgtg cagattgact aattcaagta
     7681 atgacatgtt cttatccttc ctttgtaagc gttttagagg cttttactcc tagtttagct
     7741 cgtaattctg ggacatacaa aatgagagcc aacaaaattg ctgaaaccaa ctttatcatc
     7801 tgtgcatcaa tatcggttgt taatatgaaa acaataatca tacggtataa aacagaacct
     7861 aatacaattg accataacct tttaccaaga ggtaagtatt ttatcattac ttctgccaag
     7921 ataattgaag ccaatccaat aacgattgta cccactccca tattcaaatc tgcatagcca
     7981 ttattttgcg ctaaaagtgc accagataaa gcaatcaggc cattcccaat catatatcct
     8041 agcattttca tccgatctac tttaatacta ttggcttgtc ccatagcctc attatcacct
     8101 gtcgctctta aagctaatcc tagttgggta tttaaaaaga gatacaaaat taagataact
     8161 aaacctacac aaacaattcc aattaataaa actgctgaaa gtttatttaa acctaatctt
     8221 gttaccattg taaccaaagt atttttgaga gcaaaagaga cgtttgatct tcctaaaaca
     8281 agtaaattaa tagagtaaag tcctgtcaac gtaataatac ctgttaggag ggctggtatt
     8341 ttcattttag tatgaagaaa acccgatact agtcctgcca acataccacc taacatgccc
     8401 gctatggttg ccacaattgg attaatatca ttaacgatac ataaggcgca aactgcagcc
     8461 cccataggga aagccccttc tgcagagaga tcggctatgt ctaagatacg aaaagtgata
     8521 aacacgccaa ttgccataat tgaccaaagt aatccttgcg aaattccgga taagataata
     8581 tctaacatac aatacctttc tgattattta ttatttttaa tacttgaaac atcaattcca
     8641 agctcttttg ccatatcttc attaactact acttttagat tttgaggata ttctgaagga
     8701 actttagcga cgtctttacc tttgaggatt ttaacagctt gtcttgccgt ttgacaacct
     8761 aaagcctcat aatcagcccc ataagaaaag agcaaaccac ttggtaccat atctgctgaa
     8821 cctcccacaa caggcacttt taattctttc gatagattcc ctaacagcgt tactgagctg
     8881 gctataatat tatctgttgg tacaaaaaga acctctgttt tagacattaa actcttagca
     8941 gtatcttgta catcattagt ggaagaaata cctttaacac ttgttttaat ccccgcctct
     9001 tggaagattt tttttgcttg ttttacctga acttctgagt tacgctcgct agtggtatac
     9061 ataataccaa cacgtttaac cttaggcatt acttttctca aaagggatac ttgcttttta
     9121 attggagaca tgtcacttgt accagtcgct aaaccttcag gttttttcat tgattttact
     9181 aattcggctg ataccggatc tgtcactgct gtaaataaaa taggtgtttc tgttgacacc
     9241 gttgtcaacg attgagccgc cggagtcgca attcccaata ctaatttatt atctttaatg
     9301 agtttttcag aaattgattg gatatttgac tgatcccctt gagcattctt atactcaatt
     9361 ttgatatttt tgccatcttt atagccttct ttagctaatt cttttataaa tcctttccta
     9421 gtggcagtta gagagggatg ttctacatat tgtaaaatac caatatgttt tacatcagat
     9481 tgaccatttt gagaagattg tgaacaagct gtcatcagaa cagatgctaa aacgctgcta
     9541 attaccatct ttgttatttt tttcattact atctccttgt aataccatca aattttccat
     9601 aaaacaaaaa accaactaga ccctacatct agttggttaa ttatttctct caattcctct
     9661 gtgcacggga gggcgaaaaa atattgcctt aaccaactag attattgaaa tgatctaatc
     9721 ggctctgctt tcatcataaa atgctttagc ttcatagata caaactttgc gtgacgtttg
     9781 tatccttgag gctacaaacg tctatctaaa gaaactaaag taataataag tatttgtttg
     9841 tttaataata gacgatttga ttaacatatt attctctcct tgtttatggt tgcctataag
     9901 tttacgtcat ttaaaaataa ttgtcaatag ttaaatttga tttttttgaa aattcttgtt
     9961 agatagataa tttaaataat agtcattcgt tgatataaca cacttttaag gaaatttaga
    10021 tttgctatcc ttaattttcc atatactcta ctaaagcata ataaatacca tcgttatcat
    10081 tatcctgagt tacatttcca attctctctt gtaaatcttt aggagcattc cccatgacaa
    10141 aacctttacc cacaatttca agcagattta aatcattata gttatcacca aacgcaatag
    10201 agtctgcaag agaaatatct aaatagtctg ctaaagtttt agcagatttt cctttttcaa
    10261 tacctggagc cattatttca ataaagtaag gagcagattg tgcgattgat aaatgtggat
    10321 attgagcttt caaagtgttc tccaattctc ccatcagaga tggttctccc atcaagagaa
    10381 gtttatgaat cctatcttgc ttctctaact ccatcaaact catctcttta gattgtaaac
    10441 caacaatctc ctcctctttt tggacccact cattttcttt atcacaggaa taccattcat
    10501 gataacgata gacattccat gcaattttcg aataatgctt gcttacataa gaacaaactt
    10561 gtaaggcaac ttgaccatcc attgtgtggg aagcaattgt tttttgttgc atatcttgta
    10621 ttaaagcccc attataggaa ataatataag aatctattcc aatttgttcc ataatcggag
    10681 taattgcctc aggcatccta gctgatatgg gaacgaaagt agccccttga gctactattt
    10741 gtttaattcc ctgccgtgtc ttaggtgtta ctttaaaatt actgttaatt aaagtaccat
    10801 caatatcact aaatattagc cttaccatat tatcctccaa aatataattg tacgcgctta
    10861 ctttagtcta acacttaaat ttttgtttcg caaataaact tcgtctggtc taatgagact
    10921 actaatataa gctatatttc aaaacaaatt agtttatttc tctcaatgaa cggtttaata
    10981 tgttactata tagtaataat aggaggatta actatgccgt atccatacaa agccaatcat
    11041 tccattgaat caatcacctt aaaagtaaat gacctcgaaa acctcgtaaa cttctatagc
    11101 gatatcattg ggttaactgt tattgacaaa tcaagcacta gagccttact aggggtaaat
    11161 caaaaaatcc ctcttattat ccttgaaaaa actgaacttg aaaagcatag tacctatggc
    11221 ctttatcaca ctgctatttt agtaccagat gaataccatc tcagtctagc tctaaatcat
    11281 ttattatctc agcatatccc tctagaaggt ggagctgacc atggctatag caatgctatc
    11341 tatctaagtg atcctgaagg taatggaatt gaaatttata atgataagga tatctctatg
    11401 tgggatatca gagagagtgg acaaattatt ggtattactg aaagactaga tattgacaat
    11461 ttacttgaca gccttgtcaa cgttcctaat aactataagc tttctgaaaa aactcgtatt
    11521 ggtcatattc atttgagtgt taaagatgct aagattagct caaaactcta tcaaaatgtt
    11581 tttggccttg atgagaaatt tgcaatcccg acagctagtt ggattgcatc cggtaactac
    11641 catcatcatc tagcctttaa taattgggct ggtccaaatc tttctaaaaa ccaagaaaat
    11701 agacccggaa tttccttatt gacaattgct tacaatgatg acaacctttt tagagatagc
    11761 ttaaaaaaag cgcaacttta ccaactgact ttccttgaaa aacaagatca ttattatatt
    11821 attgaagatt ttgatggcat ccgaattaaa gttgttctaa aaccagagaa ttaaccatca
    11881 cttgactttt gactcttcat tttacataat gtcaatatga taaaaatttt gatcccaact
    11941 gcaaaagaaa tgaaagtgtg tcaaaacatt gcaaggccta aactgagtgc acaaacgcaa
    12001 attataattg attatttttc tactttaact gtttcagact tagaggatat ctacagaatt
    12061 aatacaagtg cagctcgttg tgaggctcaa agatggcaag attttaaatg taaacaactt
    12121 actctgaatc ctgctattaa gttattcaac ggtcttatgt accgcaatat caaaaggcac
    12181 aacttatcca caagtgaagc acaatttatg gaaaattctg tttttattac tagtgctctt
    12241 tatggcatca ttcctgctat gacactaata tcccctcatc gtttagattt taacactaaa
    12301 atcaaaatca acaataactc tcttaaagtt ttttggcgtg aaaattacga tacttttatg
    12361 cagtcagatg acattatggt ttctttgctt tctaatgaat ttaaaaccgt cttttctcct
    12421 aaagagcgtc aaaaactaat acatcttaac tttattgaag atcgagatgg ccaattaaaa
    12481 acccactcta ccatatcaaa aaaagcaaga ggaaagtgtt taacttctat gatggaaaat
    12541 aattgccaaa ctctagaaca cttaaagcaa ttacgctttg atggcttctg ttatgataat
    12601 gaactctcag actctaagca attaactttt gttaaaaaac agacttaagt aactatttat
    12661 ccacaattta ttcacacaaa aagcaagaga taatcgtatc tcttgctttt tgacaaactt
    12721 gtaaggtcta aaaacctctc tatatcaagg tttcaaataa tattaacaaa aaataattgt
    12781 tatcaacaac ttgtggataa ctatggggat agacagagga taatctgttt atataaagtt
    12841 gattacttaa cagtaatgtc attgtatata cctttagatt tcaaaaatac ttgttaaatt
    12901 aacaataata aacgtcaaca tcttgtcaca gactgtcaaa tacttataaa caagctgtgg
    12961 ataaacataa ctttcaattt ctatttaaaa gttaattttt gacactaaag taaataatct
    13021 cgtttgactt gttcataact attgtcacca tcgtttaatc cttcccaaat cacgacttca
    13081 ccttctttta aagatttttg tacatcaatg attctttgat ttgaagatcc tctaaattgt
    13141 agcattaaat ttcgcttact ttgatcaaac ctaccatcca ctaaaatatc aatcaaagat
    13201 aacatctcca atttatcttg agtctccagc atcatttctt cccaagtata ccccgtccat
    13261 gaccaaatat ctttttctgg caattcacgt cggatacgct gaagaagtgg cagcaaaata
    13321 cctgtattta agaacggctc tccacctaat aacgttaaac cttggacgta aggttgtgcc
    13381 aaatctttca taatttgatc ttcaagctct tttgtataag ggattccggc tcgaaaggac
    13441 catgtagcag tattataaca ccctttacag tggaacatac atccagctac gtagagtgaa
    13501 tttctaacac cttcaccatc tacaaaatta aaagctttat aatcaatgat atggccatga
    13561 cttaattctt cgcttttcca ctccccaggc tttggagtat tccaacttgc ttcttctgac
    13621 atttctaacc tctctattga tatgaaaaga accagaacta actggtcttt ttattattat
    13681 cttaaagtac tatcttaggt tatccaataa cgctctgtct gatgacgaca atcttctaaa
    13741 ataccaccgt tagctaatat tactgacttg ctggcatcat tgtctacatg gcatgtcaca
    13801 agtatttcat tgatattttt tagatgtgct tgctcaattc ctagcttcaa ctgttcttta
    13861 gcataacctt taccacgctg gcgaggtcgt atggaataac caatatgtcc acctttttct
    13921 aaaagagtat catttagtct taaacgtaaa tttaagaaac ctatagcatt attgtcattg
    13981 tcaaaagaca caaactgaat atatggaaca aaattatcag gaacacctat ccctaactcc
    14041 atatttaggt tatgatctag ccagtcttca aaatgcgtag ttggttcata aaaaccacca
    14101 tccattgaac tactttcttt ttgaaactct ttatacatgg ctaaaatttc ttctttatct
    14161 tctaacactg ggcgccgtaa tttcatattg tttcctattc cattatcttc aagttaagca
    14221 ttccaaaagg agcattcgct ttattaatct ggtcacaaat aaaatgactc aataaactag
    14281 actttggtga tataggattg acacctgtag tgatagcttg aataaaagca tcaatcattg
    14341 gttcaaaacc acgtttctct agagtgcttg cccaagaacc aaaaccgata gcacgacggt
    14401 caaagccatc aataacagaa agatttgaca aatcatctaa tgaataggtt acttctggcg
    14461 actctatttc aattatttct cgacgagacc ccgactgcag gttcatgctt gcttctatta
    14521 tctcactgtc tgtttctagc gttactgata cttgtaatat cttattccca tcacgtttag
    14581 tcacaaagta tcctctttta acaacattat ttgtcaaaaa tagcgccgta tctaaaggat
    14641 ggataaacat atcaaaaagc ttatattgaa aatccgctgg tgcattgaca gcatttttaa
    14701 aggtgcgaat atggttttta tcttctactt ttttcatttc cataatacgt ggtgcaaagc
    14761 gacgattaaa acctgccatt aaaaatgttt tatgatcctt cgccaaatca tacaatgctt
    14821 tagttgaagt ataatcttct gtcaaaggct tatccataaa aactggaata ccctttttta
    14881 aaaaaagctt tgctatatca aagtgagcac tagtcgcgac atgaatgaag actccatcca
    14941 aaggatgctc ggctagacta tctaaactgt cataagctgt agaagaacca aaaagcatat
    15001 taacttcttc taaaatctgt ttttggcgtg taaaaagatg ccagtgcaca cctgtcactt
    15061 gacgcatata aggtagataa gctttctgtg aaatacctcc taaaccgaca attccaagtt
    15121 gcaacatagc atcctccttc tattttttct ttgccttttc caaaaattgt ttccttaaat
    15181 cttttagacg tgatttttga ttagcattct ctccagtaga gtgcttctca tggaacttct
    15241 gtacttgtgc ttgccctttg taatctaatt ggtatttccc catatcttaa taactccttc
    15301 tattttatct tcttatttta acatataata gaccagaggt tttaatagag gttttaatta
    15361 attaccttga tttttaatac ttgatccgtt catatgtttt acacgagcag agatttcctt
    15421 atgacgacca ttaaccatcg gacgagcctg tggattacct aagtaaccac atgtacgttt
    15481 gacaacatct actgtcttag ggtcgctgtt accacaattt ggacaagtaa agcctcgatc
    15541 tgtcggtgta aaatcccctt caaattgaca ttggtaacac ttatcaattg gtgtattagt
    15601 acctagataa cctacccgat cataagcata gtcccaaact gcttctaaag cttttggatt
    15661 ctgttgcaaa acagggtatt cacagtagtg aataaatcct cctgatgcac ccgtttcagg
    15721 atagattttc tcaaaatcta gtttttcaaa aggtgttgga ttcttacgca catcataatg
    15781 gaatgaatta gtgtagtatt ccttatcagt aatatcagtt actataccaa atttttcagt
    15841 atctaaacga caaaaccggt cagtcaagct ctccgatggt gtcgaataaa ctgaaaaatg
    15901 ataatcatac tcatcagacc aatctgcaca taactgcttc atcttacgaa cgatgtctac
    15961 agtaaatgct ttagcttgat gattatcttc ccagtcacca ccataaaaaa cactcgccac
    16021 ctcatataaa ccaatataac caagagacac cgttgcacgg cggtgattaa agagtcgatc
    16081 aacattctca tattttccta aacgttctcc aaatgctcca tactggtaaa gaatgggcgc
    16141 atttgctggg atagcctctt tgacacgttc cacacgataa accaaggcat ctctagcaat
    16201 actcatacgt tcattaaata tctcccagaa tttatccata tcaccgttag actccatggc
    16261 aacacgcggt agattaactg ttacaactcc aagattcata cgaccagatg ttacatcctg
    16321 accatttgca tctcgccacc cttgtaggaa ggaacgacag cccatagggg ctttaaaaga
    16381 gcccgttaag tcaataattt tatcataaga aaggacatct ggatacatcc gttttgtcgc
    16441 acactctaat gctaattgtt taatatcata attggggcta ttttcttcta aattaagacc
    16501 ttttttaagc gtaaaaatta attttggaaa aatagcagta cgatgctcgc ttcctaaacc
    16561 ttgaatccga attttcaaaa tcgctttttg aatttctcgt tcaaaccaat tagttcccaa
    16621 tccaaaacca agcgaagtaa atggcgtttg cccatttgaa gtaaataaag tattaatctc
    16681 atactccaaa gattgcatgg catcatatat atctttttgt gttttagcgc gcgcatagtc
    16741 ctcttgttta tcttcaataa cccattcttt tgcatctttt aaatgttttt gataatttaa
    16801 ctgtgcataa ggagctaaaa actcatcaat gcgatctgct gtacagccac catattgact
    16861 tgaagcaacg ttcgcaataa tctgtgaaat ctgagcagtc gctgtctgaa tagattttgg
    16921 actctcaact tctgcattac caatcttaaa accatttgca agcattccct taaaatcgat
    16981 taaacagcaa tttgtcatgg gagtgtatgg gctgtaatct aaatcatggt aatggatatc
    17041 acccttttga tgtgcatttg ctacatgaga aggtaacatt ttcagaccaa ttgatttccc
    17101 tacaatacct gctgttaaat cacgttgggt attaaacaca tcagagtctt tattagcatt
    17161 ctcattaaca acagtttgat ctttattaat taattttcca atagaaaaat tgatatctgt
    17221 agcctgtgaa cgctcaaaat cgcgttgggt acgataattg atatattctt gtgcaatagc
    17281 gtactcatta gcttccaata atttatgttc aacgatattt tgtatttcat atatcttgat
    17341 gttatcttta aaacgattgt agatctcagc gatgacatga tctgcaatca tctcaagttt
    17401 agcctcaact aaaggagaca ttttgataac tttattacta gcttttaata gagccgtgta
    17461 gattttatac ttatcaaagc tgaccaaacg cccgtcacgt ttaatcactt tgatatcaga
    17521 ttctgtcata aatcgttctc tttccaatac catcatgtga aatatcctcc agtgacaatt
    17581 atagcatgca aagaaaaaaa atcaatatat tgtgtcaagt tttttgctca gacacaatat
    17641 attgattttt taatctattt gaaacgatat agaacaaaat gagtgatatt tgatagagga
    17701 acttcttggt agtgcttctt aatatcagat ttcagttcat ttggtaactt ctcattttta
    17761 tttaagataa agtatttagc cccatgctga agcagctcgt ccttcaaaat tttctcatta
    17821 tttttttgcg ctgtattaat atgtggcaaa acaaattgcg aagcagattt taaccgagtc
    17881 tggctataaa tgctagcaac tttgtcccaa acataaatct taccatctct atcaattttt
    17941 gtagtcaaat aatgtgaaat gtcacgcctt tctttactaa gagtaacatt ttgataagta
    18001 ttataaacaa gtaaccctat caataaggaa actagtaaga ggggcaaata atatgttttc
    18061 tttacaaaaa ggtctacgat aagagattta ccagagaaat actgacgtct atgtgaacgc
    18121 ctgtcaatca ctttttggta aagaatagta attttattac tcgttagcaa aagaccaaat
    18181 ggtaaagcag gtaaaaagtg gtaaaggtta aaatctcttg aaaaaatggc cattatagcg
    18241 taaataataa aaatacaggc aataaagttc aagccgattt gataagatgc ttttttacga
    18301 ttttgaataa aatcccatag tccaaagagg caacctgacc ctagaaggac aacaatttga
    18361 attgctaaac taagaaggaa tgagtggttt gtcctagcga aatacgtaaa ggggtaaaaa
    18421 atagctttgt caatataaga agagataatc tgtagattaa acataaagta accaacagta
    18481 taagcaatta aaatcatacc aaaaacaacg cataaaaact gatagaaccc tcgtagtgac
    18541 tgcttttgct taatattggt tgctgttaag agaacaaaag agcaaagcca aaaaatcagt
    18601 gtttggggat ctataaatag tgagaatgct gctaaaatac cataaaatat aaatgcttca
    18661 tcaacgatat tatcgacaca aaatttaatt aaaaaccata gcgaaatgag cataaaaggt
    18721 aatgcccact gtattgggta cataccaccg aatcctaaga caatattcat tatatagaaa
    18781 atcaacgtca tgcctatcga gacaatttca ctttgtgcca cataataaac taatttataa
    18841 aaataaattc cagatacata gtaagcaata aactgtacaa taaataacca gatactagac
    18901 cctaaaaggt aacttagagc aatcgttaca tagtataaaa atcctcctgt agcaaaaaca
    18961 tcactataag ggatctgtcc tttggtcatc atcaaacctg tatagagatt ctgagtctgc
    19021 aaaccatttg caaaataagt caataaagga tttgcaattg ctaagagagt tgacaaaaca
    19081 ctatatagta aaattgaaaa tcgactagat ttcttcattc tgatagccgt tcgtttactc
    19141 attgatgggc tatttttata gctcactcca attggttttt ctaggttttc taccactatt
    19201 tttctcctca tatttacata ctaccagtat atcagaaaac cttatttagt caatttagcc
    19261 aacacttgac cgtaacgaat aatatcccta aagtagtggt atcttctaga catatattgt
    19321 ttgagaaatt cttgttcttc taatgttagc tgagtatctg acattaacat cacctccttt
    19381 tactacatca tcactattat tattcgaaaa aaaaaacgac tgaacaattg ctcaatcgct
    19441 tcaatattat tcttcatcta gaaagctgtt gaaaacttcc tcaatcatgt cccattctgc
    19501 atctgaatct tctggtatag gctgaaggtc tccttcagtt ccatcagcat tttctgtaaa
    19561 tgagtaagct tgaatctcaa tttcaccttg ttcgtcttct tctgcacctg caggaacgag
    19621 gagaacatag tttttgccaa actcttcacg tccatcaata gttagtagga tttcaaaaag
    19681 tgtttcgttc ccattctcat ctacaagagt gattacttca tgttgatgtt catggttatg
    19741 accgtggtta tgattatgtg ccattgtttc tcctttttat gccgaatgct taaaacatgc
    19801 ggtctaaata attttgtaag attagttgag ctgctaattt atcgataact ttcttacgct
    19861 taccacgact aatatcagct tgctcaacta gcatacgctc tgcttgaaca gtcgttagac
    19921 gttcatcttg atattcaact ggaagattga ataattcagt gattttatcg ccatatacct
    19981 gactagcctc tacacgagga ccacttgtat tattcatatt tttaggtaga ccaactacaa
    20041 atttatcaac tttgtactct tttactaact ctgctaagcg gtcaaaacca aaatttccag
    20101 attcttcatc gatcttaatg atttcaagac cttgtgcagt gaaaccgagt ggatcactaa
    20161 ttgcaacgcc aacagttttc gagccgacgt caagtcccat tattctcatt acaaatctat
    20221 tccatttcct tgtaaataat aacgaacgag ctcttctaca atctcatcac gttcatattt
    20281 acgaatttga ttgcgagcat cattgtaacg aggtacataa gcagggtccc cactcaatac
    20341 gtaaccaaca atttgattga tgggattgta acctttttct tctaatgaac gataaacagc
    20401 tgttaatgtt tcactaattt ctactttgtt actatcatct aatctaaaac gaacagtttc
    20461 atctgtaaat cccataccta caccttcttt cctttaattc tttcacttat tataacttaa
    20521 ataaagtatt tgtacaagaa attgataaac actatattag aaaatcaaaa agggagcaca
    20581 agaaaccaag aggtgattct ttgatttctt tactccctac cttataggtg atttttacag
    20641 gttatcatgt gactctcact tatgcttatc taaagctcaa agtgctgcac gaagacgagc
    20701 ttctgcattt tcaacgttac gaatagagcg aggtagaaaa gcacgaatat catcttcctt
    20761 ataacctact tgaagacgtt tgtcatcaat tagaattgga ctctttaaaa tacgtggatt
    20821 ttcttgaata agatcaataa cttcatttac actcaactcc tcaatattac aattgagcgc
    20881 cttagcataa cgatttttag aagaaacgat actttcaata ccactttcag tttttgttaa
    20941 tatttctaaa atttcatcac gtgttagtga ttctttacct aggttttgtt ctttatatgg
    21001 aagttgatga gcatttagcc aagtttttgc ttttttacaa cttgtgcaac ttgaaatagt
    21061 ataaatctta atcatgtgcc ttctcacttt ctatcccatg atagttatag tttaacatag
    21121 tcaca
//
LOCUS       DATRNX010000027        20686 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRNX010000027 DATRNX010000000
VERSION     DATRNX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBC81_000811"
     CDS             complement(34..744)
                     /locus_tag="VBC81_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6911866.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBC81_000812"
     CDS             complement(741..1439)
                     /locus_tag="VBC81_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6911867.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBC81_000813"
     CDS             1607..2371
                     /locus_tag="VBC81_000813"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6911868.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSLEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBC81_000814"
     CDS             2479..4986
                     /locus_tag="VBC81_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6911869.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBC81_000815"
     CDS             5072..6265
                     /locus_tag="VBC81_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6911870.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBC81_000816"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBC81_000816"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6911871.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBC81_000817"
     CDS             complement(7672..8229)
                     /locus_tag="VBC81_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6911872.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBC81_000818"
     CDS             complement(8226..9209)
                     /locus_tag="VBC81_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6911873.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBC81_000819"
     CDS             complement(9455..9868)
                     /locus_tag="VBC81_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6911874.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC81_000820"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC81_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6911875.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBC81_000821"
     CDS             10909..11883
                     /locus_tag="VBC81_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6911876.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBC81_000822"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBC81_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6911877.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBC81_000823"
     CDS             12806..14203
                     /locus_tag="VBC81_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6911878.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBC81_000824"
     CDS             14345..15865
                     /locus_tag="VBC81_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6911879.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBC81_000825"
     CDS             complement(15978..16238)
                     /locus_tag="VBC81_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6911880.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBC81_000826"
     CDS             complement(16347..17282)
                     /locus_tag="VBC81_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6911881.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17548..18570
                     /locus_tag="VBC81_000827"
     CDS             17548..18570
                     /locus_tag="VBC81_000827"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6911882.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18629..19072
                     /locus_tag="VBC81_000828"
     CDS             18629..19072
                     /locus_tag="VBC81_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6911883.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19149..19424
                     /locus_tag="VBC81_000829"
     CDS             19149..19424
                     /locus_tag="VBC81_000829"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6911884.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19417..20613
                     /locus_tag="VBC81_000830"
     CDS             19417..20613
                     /locus_tag="VBC81_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6911885.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
BASE COUNT         6649 a         3269 c         4154 g         6614 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcactt gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg cccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt ataaaattcg gatactgcta actaacaatc
    17461 ttttttatga taatattttg taaatgtatt gctttttagc taacttttgt taatatagta
    17521 aaaatgcttt ttaataggag ataaattatg acacaagcag tattaaaaga attggctaag
    17581 gcggagttgc attgtcatct agatggctca ctttctttac cagctattag aaaattggct
    17641 aatatggctg atataatact gccaaatagt gataaagaat taagaaaata tgttatagct
    17701 ccagctcaaa cagaaagctt ggtagattac cttaaaacgt ttgagtttat cagaccgctt
    17761 cttcaaacga aggaagcatt acgttttgct gcttatgatg ttgctagaca ggcagcttta
    17821 gaaaatgtta tttatattga aattcgtttt gcgccagaac tctctatgga taaaggttta
    17881 accgcaagtg atacagtctt tgcagtctta gaaggtttag cagatgctca gaaagaattt
    17941 aatatcgttg cacgcgccct tgtctgcggt atgcgccaat cgtctcataa aacgaccaag
    18001 gaaattatta aacacattgt tgatttggct cccaaaggcc tggtaggttt tgattttgca
    18061 ggtgatgaat tttcctatcc aacagatagt ttagtagatc ttattcagga agtaaaacgc
    18121 tcaggttacc cgatgacttt acatgcaggt gagtgtggtt gtgctaagca tattgcggat
    18181 agtttaaatt tagggattaa gcgtatggga catgtgacag ctttaacagg acagagggct
    18241 ttaataaagc gatttgtcga agaagatgct gtagcagaga tgtgtttaac tagcaattta
    18301 caaacgaaag cagcctcaag tattcaaagt tttccttatc aagaacttta tgatgctgga
    18361 ggtaagatta caattaatac agataatcgt actgtttccg atacaaattt gacgaaagaa
    18421 tatagtcttt tcgtaactta ttttggaacg aaaatagaag actttcttgt ttttaatcaa
    18481 aacgctgtga aggcctcctt tacaagtgat tcagagaaag atactttact acataagcta
    18541 caagaaaact atgacagcta tctaaaataa ccttcacgat tctttaaaaa tatgatagaa
    18601 tagtggtaat ttacaagtgg aggaagatat ggctttagca aagattgttt atgctagcat
    18661 gaccggaaat actgaagaaa ttgctgacat tgttgctgat aaattacgtg acttgggtct
    18721 agatgttgaa gtagaagagt gtacgatggt tgatgctgca gattttgaag atgctgacat
    18781 tgcaattgtt gccacttata catatggtga tggagattta ccagatgaaa tcgtggactt
    18841 ttatgaagat ttagcagaag ttgatttatc tggtaaagtt tacggcgtag tgggttcagg
    18901 tgatactttc tatgattact tctgcaaatc tgttgatgag ttcgaggctc aattcgcatt
    18961 aacaggagca caaaaaggtg ctgattgtgt taaagttgat ttagcggcag aagacgaaga
    19021 cattgaaaac ttagaagctt ttgctgaaga aattgcttct aaacttaact aagctaccaa
    19081 aaagacgaac acgagtcgtc tttttaactt ttttaccatc ttttgtaact agaatatgag
    19141 atatagatat ggaattagaa gagattcgtc aagaaataga tgaaatagat cagcaattgg
    19201 taagcctttt agaaactaga atgggtctaa ttttagaggt catagcattt aaaaagaaac
    19261 accgacttcc agttttagat aataatcgtg aaaacgaagt gcttaacaat gtattgaaga
    19321 aagtccaaaa tcatcaattt gacgatgtga tacgagcaac tttcaaagac attatgacag
    19381 aatctcgtgt ttatcaaaag gagaatattg tagacggtga ttaaacgatt tgataattat
    19441 gctcagtacg tacttgtttt agggtttagc ttactaatcg gtggtgtggt tggggctatt
    19501 acagcagtct ttggacgtgt tctattattc ctgacagcct ttagaagtga ttatatagct
    19561 tacctactcc cttttcttag cattgttggt ttatttatag tttttgtcta tcaaaaattt
    19621 gggggtaaga gtgttaaagg gatgggattg gtatttgaag taggtcatgg taatgaggag
    19681 actattccga aacgtcttgt tcctctcgtg atattaacaa cttggttaac ccacttattt
    19741 ggtggtagtg caggtcgtga gggggttgct gtacagattg gggctaccgt ttcacactac
    19801 tttcaaaaat attgccgttt acaaaacgct tctcaacttt tcctagtaat gggaatggca
    19861 gcaggttttg ctggtttatt tcagactcct ttagctgcta ctttttttgc gatcgaagtt
    19921 ttggtagtag gtcgtttaat ggtttcgtat atattaccaa gtttaattgc tgctctgaca
    19981 gcaaatttcg tctcacattc tttaggttta gaaaaatttt ctcatagtat agcaacatca
    20041 atggcgttaa caccagatat tattctaaaa ttattggttt taggactatg ttttggtctg
    20101 tgtggcaatt tatttgctta tttattggct aaagctaaat taattgcttc tagtaggtta
    20161 ctaaacccat ataaacggat tttcacactt ggattgttag ctactttttt attgtttatc
    20221 ttccattttg gacgatattc aggactggga acaaatctaa ttgaggctag cttcactaat
    20281 aagaatcttt atgattatga ttggttgtta aaattgtgtt tgacagttat tactttggct
    20341 gcaggttatc agggaggaga agtaactccc ttatttgcaa taggagcaag tttaggagtg
    20401 attattgcgc ccattttagg tttgccagtt attttagttg ctgctttagg ctatactagc
    20461 gtttttggga gtgcaacgaa tactctgtta ggtcctatct taataggtgg cgaagtcttt
    20521 ggctttgcca acactcctta ttttgttatt gtatgtcttg ttgcttactc catatcacat
    20581 gctcatacga tttatggtgc tcaatctaga tagttagtga tgagttttaa aagagtttat
    20641 cagtttatag aacggaatat tccgttctct tttttattct cttaaa
//
LOCUS       DATRNX010000028        20599 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRNX010000028 DATRNX010000000
VERSION     DATRNX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20599
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(67..348)
                     /locus_tag="VBC81_000831"
     CDS             complement(67..348)
                     /locus_tag="VBC81_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911886.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
     gene            complement(382..768)
                     /locus_tag="VBC81_000832"
     CDS             complement(382..768)
                     /locus_tag="VBC81_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911887.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            complement(753..1433)
                     /locus_tag="VBC81_000833"
     CDS             complement(753..1433)
                     /locus_tag="VBC81_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911888.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            complement(1379..1966)
                     /locus_tag="VBC81_000834"
     CDS             complement(1379..1966)
                     /locus_tag="VBC81_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911889.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            2375..2689
                     /locus_tag="VBC81_000835"
     CDS             2375..2689
                     /locus_tag="VBC81_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6911890.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2705..3091
                     /locus_tag="VBC81_000836"
     CDS             2705..3091
                     /locus_tag="VBC81_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6911891.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            3120..4505
                     /locus_tag="VBC81_000837"
     CDS             3120..4505
                     /locus_tag="VBC81_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6911892.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4508..4660
                     /locus_tag="VBC81_000838"
     CDS             4508..4660
                     /locus_tag="VBC81_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6911893.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4683..5888
                     /locus_tag="VBC81_000839"
     CDS             4683..5888
                     /locus_tag="VBC81_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6911894.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            5931..6152
                     /locus_tag="VBC81_000840"
     CDS             5931..6152
                     /locus_tag="VBC81_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911895.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6269..6766
                     /locus_tag="VBC81_000841"
     CDS             6269..6766
                     /locus_tag="VBC81_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6911896.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6741..7247
                     /locus_tag="VBC81_000842"
     CDS             6741..7247
                     /locus_tag="VBC81_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6911897.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7231..9678
                     /locus_tag="VBC81_000843"
     CDS             7231..9678
                     /locus_tag="VBC81_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6911898.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9681..11858
                     /locus_tag="VBC81_000844"
     CDS             9681..11858
                     /locus_tag="VBC81_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6911899.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11855..12856
                     /locus_tag="VBC81_000845"
     CDS             11855..12856
                     /locus_tag="VBC81_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6911900.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12853..13788
                     /locus_tag="VBC81_000846"
     CDS             12853..13788
                     /locus_tag="VBC81_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6911901.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14033..14149
                     /locus_tag="VBC81_000847"
     CDS             14033..14149
                     /locus_tag="VBC81_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6911902.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14165..16084
                     /gene="tet(M)"
                     /locus_tag="VBC81_000848"
     CDS             14165..16084
                     /gene="tet(M)"
                     /locus_tag="VBC81_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6911903.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16203..16370
                     /locus_tag="VBC81_000849"
     CDS             16203..16370
                     /locus_tag="VBC81_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6911904.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(16430..16783)
                     /locus_tag="VBC81_000850"
     CDS             complement(16430..16783)
                     /locus_tag="VBC81_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6911905.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            17288..17710
                     /locus_tag="VBC81_000851"
     CDS             17288..17710
                     /locus_tag="VBC81_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6911906.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            17707..17937
                     /locus_tag="VBC81_000852"
     CDS             17707..17937
                     /locus_tag="VBC81_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6911907.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            18398..18601
                     /locus_tag="VBC81_000853"
     CDS             18398..18601
                     /locus_tag="VBC81_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6911908.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            18683..19900
                     /locus_tag="VBC81_000854"
     CDS             18683..19900
                     /locus_tag="VBC81_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6911909.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(20022..20414)
                     /locus_tag="VBC81_000855"
     CDS             complement(20022..20414)
                     /locus_tag="VBC81_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911910.1"
                     /translation="MQKSSKIILLIVALLLLIFSGGFYMKEQQRKEELKRNREYEVSL
                     VKALKNSYSDIKEIQISNPITTTMPGDWRSTVKIIFSDNDEITYCITHNKDDEINRSG
                     SINEKEHLFLSKTKYKFLIIFLYFLKCS"
BASE COUNT         6632 a         3711 c         4078 g         6178 t
ORIGIN      
        1 aacttgatta ttttcatatc tataataact atcaggatag taagcttcct caacattttc
       61 actcgattat aaatcatcat aactaccttt atttaaatct acctgtgcct ttactttttc
      121 ccaaaattgt cctacaagtc cagtgtcaat ttggtttttt atcgttttta ttgatgattt
      181 agcagtctca gaaatatcat caatcttttc actataagtc gtcttaatag aatcagcttc
      241 ttctactatt tccggggcat ggacaaacct tactaaagct ttttgaattt gctttttgaa
      301 ttctttgtct tctttggttc ctttcaatac agcgcctatt aaactcattt attgcctctt
      361 tcactttata gtccatctaa attagtagta ttttcttcct tctaaactag aaatgtcatt
      421 ttctaatttt actcggtctt ttagaaactg tgtccgacta ttttcctctt ggacaatgat
      481 agtctgttta tagttttcat attcatcaat taattccatt tgataacttt caattattcg
      541 atttaaactc ataaaagtcg aaccatctga atcattttgt ctcaaaaagt gacttgtctc
      601 ttcatagact tcttgtaaat aagtctctaa ctcgttcttt ttatcctcaa ggctatataa
      661 tttatcttct atctccatat tttcgcgatg ataattatct tccaatttcc ctaggtttat
      721 ttttagacta ttaattttgt tttcattttg atctagtatt gtgaccattc cttaaattct
      781 cctgctaatt tggagtcctc ttctagcaat ttttggacac cagattcaat ttgtttttga
      841 agattagtat atgaagttgt gatctcttgt gcattagtaa ctttttcatc aaagaaagtt
      901 tcaattggtg ttactatggt gtcctctcta actccacccg cactatatgc atcagctaat
      961 tcttctggac ttaaatctgt tataccagga ggcatcactt tagtttttgc aaatagttct
     1021 gatgcttttt ctttagctaa ttcagcattc ttcttgatat catcagaagc tgtttgagat
     1081 gccgtggtta aaccgttagc aataaatata gcctgttcag aatctaaata tactttttca
     1141 ctatgactaa gtttgcctcc agattttttt aatttagctt tagctttttg aaaatgaact
     1201 tcttcataaa gaacagactg aacagctggt gacttaggat ttacgatgtt acctttttca
     1261 tcttttaact gaccatcatc agtaaatttc caactctcta ttttatgact tttaccattt
     1321 acccatttca cggtaccata acctttatca tctagtttat taaaatcgtt ccaccaagtt
     1381 acattatcct ctttatgtcg aaaattagca aaatattcag gatgcttagc catgaatttt
     1441 tttcatcttc atttaaagtg ctatacctaa accatccatt aaatactgta gtcgggatat
     1501 gatatttagc acctaatttt agcatataag cagattgaga atagcctgaa agctgagtca
     1561 cagtccattt gtcatgtgac tgtacgtctt ttaaaaattt atcagctgat tttaactgag
     1621 tcgaactttg atgtggttga gcagatgaaa ctgcgccaat taaatcttta taattacttg
     1681 aatccgttgc ggcggaaata actgcaacac tgttataatc tactttaccc ttgataattg
     1741 gtgcaactgc cataccctga tagccagaca tatcgtcagg agaagttttt aataccttaa
     1801 acgctttacc cgtctttgtt actattaaat ttgtttcatt agattttttt ggattagctg
     1861 ttattatttt tccgccattt tgtttcacat agtttggatt tacactatat acgttctcac
     1921 cttgattcaa tctatttaaa tcatactcgc tataagaata tgtcattatc ttttttcctt
     1981 ttctcctgta gaaaactcaa ctaaaatatg attttttgtt cttccatatt tttaatttaa
     2041 aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact ttatcaaggt
     2101 caaaaccact caatttacta ctaatttact acttatgaat gagctttgat acgacgattt
     2161 atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt taataggtag
     2221 acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag tgaacttatg
     2281 aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat caaacattca
     2341 ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg tgattcccaa
     2401 catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg ttcagcgaag
     2461 aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg atgttcaaag
     2521 agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt tcggctttga
     2581 ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa tcggcactcg
     2641 tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa gaaagagagg
     2701 aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt ttggagaatt
     2761 gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt cggtttcaga
     2821 tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca tgattcaagt
     2881 aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg tggaacttat
     2941 caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg ttgactggta
     3001 tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag ctcaaccaca
     3061 agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga ctttttcgca
     3121 tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt cattttacga
     3181 tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg aagacaatcc
     3241 agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac attccctatt
     3301 taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt gtattcaaac
     3361 gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca aagatgatac
     3421 ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt aaagatagtg
     3481 ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga cttaaaaatg
     3541 gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa ctcttacact
     3601 tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag ttaaaggatt
     3661 cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct attgatgaag
     3721 tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa tatgataagc
     3781 tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt atactgacac
     3841 tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg aaaaatgctg
     3901 accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa gacttgcttt
     3961 cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg aagcagatga
     4021 agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac ttcttaatct
     4081 ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc gcagttatga
     4141 ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta atactggctt
     4201 gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt aatttcagag
     4261 tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt gacgtacaaa
     4321 aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga acaagtgtca
     4381 tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag gaaattaaaa
     4441 agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc gcaggtgtgg
     4501 actgatcttg ctggctggtg tggcaatagc cacgccagca cttaaccccc cgtatctaac
     4561 aggggggtac aaatcgacag gaaacagtca aaaaaacatt agaaaatcct ttggttacaa
     4621 gggatttaca aaatttcagc gtatgtcaaa tgggctttaa aagttgacat acgccttttt
     4681 gattggaggg atttttactg aatgaacaaa cttggttaca gcatttaaaa gaaaaacgct
     4741 tggcttatgg actatctcaa aaccgtttag ctgttgcgac tggtattaca aggcagtatc
     4801 taagcgatat tgaaacagga aaagtcaagc catcagagga tttacagcag tccctttggg
     4861 aagctctgga acgcttcaat cccgacgctc cccttgaaat gctgtttgat tatgtaagga
     4921 ttcgctttcc gacaacagac gtacagcagg tggtcgaaaa catcttacaa ctgaaactgt
     4981 cctattttct tcatgaggac tatggtttct attcttattc agagcattat gctttaggcg
     5041 acatattcgt cctttgctcc catgaactgg acaaaggagt tctggtggaa ttgaaaggtc
     5101 gtgggtgcag acaatttgaa agctatcttc tggcacaaca aagaagctgg tatgagttct
     5161 ttatggacgt tttggtggct ggcggtgtga tgaaacgcct tgaccttgcc attaacgata
     5221 agacagggat tttgaatatc cctgtactca ctgaaaagtg ccaacaggaa gaatgtatct
     5281 ccgtcttccg cagttttaaa agctatcgca gtggcgaact ggtacgcaaa gaggaaaagg
     5341 aatgtatggg aaacaccctc tatatcggtt cattacaaag tgaagtttat ttctgtatct
     5401 atgaaaagga ctacgagcag tacaagaaaa atgatattcc cattgaagac gcagaagtaa
     5461 aaaaccgttt tgagattcga ttgaaaaatg agcgtgccta ttatgcagtc cgtgatttac
     5521 tcgtctatga caatccagag cataccgcct ttaaaattat caatcggtat atccgttttg
     5581 tagataaaga cgattccaaa cctcgttctg attggaaact gaatgaagaa tgggcttggt
     5641 ttattgggaa caatcgtgaa cgattaaaac taaccacaaa accagagcct tactccttcc
     5701 aaaggacgct gaactggcta tctcatcaag ttgccccgac cttaaaggtt gcgattaaac
     5761 ttgatgaaat caaccagacg caggttgtaa aagacattct cgaccatgcg aaactgacag
     5821 accgacacaa gcagattttg aagcaacagt cagtaaaaga acaggacgtg ataacaacaa
     5881 aaaaataact caaatacaaa ttcattgaat atagagagga gaacattttt atgaattttg
     5941 gacaaaacct ttataactgg tttctatcaa acgctcaatc actggtgctt ttagcaatcg
     6001 ttgtgattgg cttgtatctt ggcttcaagc gtgagtttag caaactgatt ggctttttaa
     6061 ttattgcgat tattgcggtt ggcttagtct tcaacgctgc tggagtaaaa gacattttac
     6121 tagagctatt caatcgcatt attggtgctt aaataaaacc gttcttttgt ggaatataag
     6181 tggttttctt atgttccgca aaggaatggt acaccaaacg aagtgcggta gggatttttg
     6241 aatctctaca aagaaaggac gtgaatatat ggacgatatg caagtctata ttgcgaattt
     6301 aggcaaatac aatgagggcg aattggtcgg tgcgtggttt acctttccca ttgactttga
     6361 ggaagtcaaa gagaaaatcg gcttgaatga tgaatatgag gaatacgcca ttcatgacta
     6421 cgagttaccc tttacggttg acgaatacac ttccattggc gaactcaatc gactatggga
     6481 aatggtatcg gaattacccg aagaattaca atcggagcta tctgctctgc tcactcattt
     6541 ttcaagcatt gaagaactaa gcgaacatca agaggatatt atcattcatt ccgattgtga
     6601 tgatatgtat gacgtggcac gctactacat tgaagaaacg ggtgctttag gcgaagtacc
     6661 agctagtctt caaaactata ttgattatca agcctatggt cgggatttag acctttcagg
     6721 aacgtttatc tcaaccaatc atgggatttt tgaaatcgtc tattaaatct gtcggtacat
     6781 tactactggc agattttcta ttttacgggg tggctcaatc agctacccct attttttatg
     6841 aaaggattga ttacatgaag aaaatacgaa gctataccag tatctggtct gtggaaaagg
     6901 tactgtattc tatcaatgat tttagacttc cgtttcccat aacctttacg caaatgacat
     6961 ggtttgtcgt gtcactcttt gcagtgatga tacttggcaa cttgccccct ctttccatga
     7021 tagagggagc atttctcaaa tactttggga ttcctgtggc tttcacatgg tttatgtcta
     7081 caaaaacttt tgatggtaaa aagccttatg gatttttgaa gtctgtcatt gcttatgcac
     7141 tgcgaccaaa gctgacctat gcaggaaaaa aagtaacgct tggcagaaac cagccacaag
     7201 aagccattac agcagttagg agtgaatttt atggcatatc caattaaata cattgaaaac
     7261 aatctcgtct ggaataaaga cggggaatgt tatgcttact atgagcttgt tccttacaat
     7321 tactcatttc taagtccaga acagaaaata caagtgcatg attctttcag acagcttatc
     7381 gcacaaaatc gtgatggcaa aattcatgct ttacaaatca gtacagaatc cagcatacgt
     7441 tctgcacaag agcgttccaa aaatgaagtc actggcaagc tcaaagcggt tgcctatgac
     7501 aaaatcgacc aacagacaga cgctttaata tccatgattg gcgaaaatca agtgaactac
     7561 cgtttcttta tcggctttaa gttgcttctc aacgatcagg agttttctat gaaaagtctt
     7621 accgttgaag caaaaaatgc tttgtctgat tttgtctatg atgtgaacca taagctgatg
     7681 ggcgattttg ttagtatgag taatgatgaa atcctgcgtt ttcagaagat ggaaaagctc
     7741 ttagaaaata aaatctctcg tcgtttcaaa atccgcaggt tagataagga cgacttcggc
     7801 tatctgattg aacaccttta cggacagaca ggcactgcct atgaagagta tgagtaccat
     7861 ctatcaaaga aaaagctgga taatgaaacg ctgattaaat actatgactt gattaagcct
     7921 actcgctgtt tggtggaaga aaaacagcga tatttgaaaa tccagcagga agatgaaacc
     7981 gtctatgtag cttactttac cattaacagc attgtcggag aactggactt cccgtcctct
     8041 gaaatcttct actaccagca acagcaattt acattcccga ttgatacgtc aatgaatgtg
     8101 gaaattgtag cgaatcgtaa agccctatct actgtccgca ataaaaagaa agaactgaaa
     8161 gacttggata accacgcttg gcaaagtgat aatgaaacca gctccaatgt ggcggaagct
     8221 ctggaaagtg tgaatgagct ggaaaccaat ttagaccaaa gcaaggaatc tatgtacaag
     8281 ctgtcttatg tggtaagggt atcagcaaat gatcttgacg aactcaaacg tcgttgtaat
     8341 gaagtgaaag atttttatga cgatttaagc gtaaaactgg tacgaccatt tggggatatg
     8401 ctcggcttac atgaagaatt tttacctgcc agcaagcgtt atatgaatga ttatattcaa
     8461 tacgtgacct ctgatttcct cgctggttta ggttttggtg ctactcaaat gctgggggaa
     8521 aatgagggga tttatgttgg ctacagctta gatactggac gcaatgtcta tctgaaacct
     8581 gctcttgcca gtcaaggggt taagggttca gtaaccaatg cgttagcgtc ggcttttgtt
     8641 ggttcgctgg gtggtggtaa atcctttgcg aataacctta tcgtctatta tgcggtgctt
     8701 tatggggcac aagcagtgat tgtagaccca aaagcagaac gtggcagatg gaaagaaacc
     8761 ttgccagaga tttcccatga aatcaatatc gtcactctga cttctgatga gaaaaacaaa
     8821 ggcttacttg acccttatgt gattatgaaa aatcccaaag attctgaatc actggctatt
     8881 gatattctga cattccttac ggggatttcc tctcgtgatg gggaacgctt cccaatcctt
     8941 agaaaagcca ttcgtgcagt aaccaatagt gaagtacgag ggttgatgaa agtgattgag
     9001 gaattacggg ttgagaatac gccactaagt accagtatag ccgaccatat cgaaagtttt
     9061 acagactatg actttgcaca tttattattc agtaatggtt atgtggagca gtctatcagc
     9121 ttagaaaaac aactgaacat tatacaggtt gcggacttgg tacttcccga caaggaaact
     9181 tcctttgagg aatataccac tatggagctt ttatccgttg ctatgctgat tgtcattagt
     9241 acctttgctt tagactttat ccatacagac cgaagcattt tcaagattgt agatttagac
     9301 gaagcatgga gctttttaca ggtagcacaa ggaaaaacac tatctatgaa gctggttcgg
     9361 gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc aaaatacaga cgacctctta
     9421 gatgaaaaac tgaaaaataa cctcggctta aaatttgcat ttcgttccac tgaccttaac
     9481 gagattaaaa agaccttagc cttttttggt gtagacccag aggacgaaaa caatcagaag
     9541 cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg atttatatgg tcgtgtcggt
     9601 gtgatacagt tccaccctgt atttgaagaa ctgctccatg cctttgatac cagaccacct
     9661 gtgcgaaaag aggtgtaaat gtgaaaccat caatagtaaa cagaataaaa tcaaactgga
     9721 cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt cacacttgtg attgccattt
     9781 ttcttttagc catgctggga acggtggttc aagctgcggg cttggtagat gatacggtca
     9841 atgtggcaaa tgaatacagc cgatacccac ttgaaaacta tcaactggat ttttatgtgg
     9901 ataatagctg gggctggctt ccgtggaact ggtcggacgg gattggaaaa caggtcatgt
     9961 atggactata tgccattacc aattttattt ggacaatcag tttgtatgtt tccaatgcga
    10021 caggttactt agtacaggaa gcctattcct tagacttcat ttccgctaca gcagattcca
    10081 ttggtaagaa tatgcagacc ttagctggtg tgagtgcaaa cggattttca acagagggtt
    10141 tctatgttgg attcctctta ctcttgattt tggttcttgg ggtttatgtt gcctatacgg
    10201 gactgataaa gagagaaacc acaaaggcaa ttcatgccat tatgaatttt gtgctggtgt
    10261 ttatcctatc ggcttccttt attgcctacg ctcccgacta cattaaaaaa atcaatgact
    10321 tttcatcaga catcagtaat gccagtttat cacttggcac gaagattgtc atgccccatt
    10381 ccgatagtca aggcaaggac agcgtggact taatcagaga tagcctgttt tccatacagg
    10441 ttcagcaacc gtggctactg cttcaataca acagttcaga cattgaaagt atcggtattg
    10501 accgtgtgga aagcctgctc tccaccagcc cagattccaa caatggcgaa gacagagaaa
    10561 aaattgttgc ggaagaaatt gaagacagaa gcaataccaa tctaaccatt acaaagacca
    10621 ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa tattgggatt tccatatttg
    10681 tattcctatt aacaggaatc atgattttct cgcaggtact ttttatcatc tatgctatgt
    10741 ttctgcctgt gagctttatt ttaagcatga ttccatcatt tgatggtatg tcaaaacgag
    10801 ccataacaaa gctctttaat accattttga cacgagctgg aatcacattg attattacga
    10861 cagcatttag tatttcaacc atgctctata ccttatcggc tggttatccg ttctttttga
    10921 ttgcttttct acagattgtg acctttgcag gaatctactt caagctgggc gatttaatga
    10981 gtatgttttc tctacagagt aacgattctc aaagtgtggg aagtcgtgtg atgagaaaac
    11041 ctcgtatgct tatgcacgct cacatgcacc gtctacagcg gaaacttgga cgttccatga
    11101 ctactctagg ggctgggtct gccattgtta caggtaaaaa aggacagtcg ggttcgggga
    11161 gttctgcaag gacacaagca gatcactccc gaccagacgg aaaggaaaaa tcaacacttg
    11221 gaaaacgtat cggtcaaacc atcggtacag tagctgatac caaagacaga atggtagaca
    11281 ctgctagtgg tttgaaagaa caggttaaag atttgccgac caatgcaaga tatgcagtat
    11341 atcaaggaaa atccaaagta aaagagaatg tccgtgattt aaccagtagt atttctcaaa
    11401 ccaaagcgga cagagccagt ggacgcaagg aacagcagga acaaaggcga aaaaccattg
    11461 cgaagcgtcg ctctgaaatg gaacaggtca aacagaaaaa acagcctgct tcttctgttc
    11521 atgaaagacc gactacaaga caagaacaat atcatgatga acagacctca aaacagtcta
    11581 atattcagac ttcatataag gaatctcaac aagccaaaca agagcgtcca gcagttaagt
    11641 ccgatttttc aagtccaaaa gtggaacgcc aaggcaatac cgttcaagaa aaaaccgttc
    11701 aaaagccagc aacttcaacc actacagcag atagaacttc acaacgtcca atcacaaaag
    11761 aacgtccgtc tactgttcaa agagtaccac tacaaaatac aagaagtaga ccaccaatca
    11821 aaaccgccac cattaagaaa gtcggtaaga aaccatgaag ttgaaaactt tagtgattgg
    11881 tggttctgga ttattcttga tggtcttctc actgcttctg tttgttgcca ttttattttc
    11941 agatgaacag gacagcggaa tttccaatat tcattatgga ggtgtgaatg tttccgcaga
    12001 agtgctggct cataagccta tggtagaaaa atatgccaaa gaatatggcg ttgaagaata
    12061 tgtcaacata cttcttgcga ttatacaggt ggaatcgggc ggtactgcgg aagatgttat
    12121 gcagtcctcg gaatccctcg gtcttccacc taattcattg agtacagaag aatccattaa
    12181 gcaaggtgtg aagtatttca gtgaattatt agccagtagc gaaaggctca gtgtagattt
    12241 agaatcggtt atccagtcct acaattatgg tggtggtttc ttagggtatg tggctaatcg
    12301 tggaaataaa tatacctttg aactggctca aagtttctca aaagagtatt caggtggcga
    12361 aaaagtgtct taccccaatc ccatagccat acctatcaat gggggctggc gatacaacta
    12421 tggcaatatg ttttatgtgc aactggtaac gcagtatctt gtcacaacag agtttgatga
    12481 tgatacggta caagccatca tggacgaagc actgaaatat gagggctggc gatacgttta
    12541 cggtggagct tccccgacta cttcttttga ttgtagcgga ctgacacaat ggacgtatgg
    12601 aaaagctgga attaacttac cacgaaccgc acaacagcaa tatgatgtga cccagcatat
    12661 cccactatcg gaagcacaag ctggcgattt ggttttcttt cattctacct ataacgctgg
    12721 ctcttatatt actcatgttg ggatatacct tggcaataac cgtatgtttc atgcaggcga
    12781 cccaatcggt tatgccgact taacaagccc ctactggcaa cagcatttag tgggagcagg
    12841 acgaatcaaa caatgagaaa ggaagattta atgatgaaat ttagaaaaaa tcagaataaa
    12901 gaaaaacaga taccaaagga aaagaaacct cgtgtctact ataaggtcaa tcctcataaa
    12961 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
    13021 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
    13081 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
    13141 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
    13201 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
    13261 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
    13321 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
    13381 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
    13441 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
    13501 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
    13561 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
    13621 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
    13681 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
    13741 gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca aatattggta
    13801 cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg agattccttt
    13861 acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt aataaatatg
    13921 cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa ttggatgtcc
    13981 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
    14041 aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata aaaatcccag
    14101 tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa tggaggaaaa
    14161 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa aaactacctt
    14221 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacagagg
    14281 tacaacgaaa acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacggc
    14341 gataacctct tttcagtgga aaaatactaa ggtgaacatc atagacacgc caggacatat
    14401 ggatttttta gcagaagtat atcgttcatt atcagtatta gatggggcaa ttctactgat
    14461 ttctgcaaaa gatggcgtac aagcacaaac tcgtatattg tttcatgcac ttaggaaaat
    14521 aggtattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
    14581 ggtttatcag gatattaaag agaaactttc tgcggaaatt gtaatcaaac agaaggtaga
    14641 actgcatcct aatatgcgtg taatgaactt taccgaatct gaacaatggg atatggtaat
    14701 agaaggaaat gattaccttt tggagaaata tacgtctggg aaattattgg aagcattaga
    14761 actcgaacaa gaggaaagca taagatttca taattgttcc ctgttccctg tttatcacgg
    14821 aagtgcaaaa aacaatatag ggattgataa ccttatagaa gtgattacga ataaatttta
    14881 ttcatcaaca catcgaggtc agtctgaact ttgcggaaaa gttttcaaaa ttgagtattc
    14941 ggaaaaaaga cagcgtcttg catatatacg tctttatagt ggcgtactgc atttgcgaga
    15001 ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa
    15061 tggtgaatta tgtaaaatcg ataaggctta ttccggggaa attgttattt tgcagaatga
    15121 gtttttgaag ttaaatagtg ttcttggaga tacaaagcta ttgccacaga gagagagaat
    15181 tgaaaatccc ctccctctgc tgcaaacgac tgttgaaccg agcaaacctc aacaaaggga
    15241 aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctgc gatattatgt
    15301 ggattctgcg acacatgaaa tcatactttc tttcttaggg aaagtacaaa tggaagtgac
    15361 ttgtgctctg ctgcaagaaa agtatcatgt ggagatagaa ataaaagagc ctacagtcat
    15421 ttatatggaa agaccgttaa aaaaagcaga gtataccatt cacatcgaag ttccaccgaa
    15481 tcctttctgg gcttccattg gtctatctgt agcacagctt ccattaggga gcggagtaca
    15541 gtatgagagc tcggtttctc ttggatactt aaatcaatcg tttcaaaatg cagttatgga
    15601 ggggatacgc tatggctgtg aacaaggatt gtatggttgg aatgtgacgg actgtaaaat
    15661 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
    15721 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
    15781 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
    15841 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
    15901 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaacttcct ttacaaatgg
    15961 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
    16021 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
    16081 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
    16141 ttaataaaga tttgaattaa agtgtaaagg aggagatagt tattataaac tacaagtgga
    16201 tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa
    16261 aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt
    16321 tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta
    16381 atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga
    16441 tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt tctttgtctg
    16501 taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag
    16561 caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt
    16621 gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa
    16681 tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta
    16741 aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac cacctctata
    16801 cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata
    16861 ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt
    16921 gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa
    16981 cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa
    17041 agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat
    17101 agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc
    17161 agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga
    17221 ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt
    17281 gagaaacatg aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg
    17341 taaacgtgct atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa
    17401 gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga
    17461 taactattca actgactttc agatttttac actcaatggg ttatcagtag gcgttgaaaa
    17521 tgatttgttg agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa ttctactgct
    17581 gttttacttt atggacatga gcgattcaga aattgcagac ctgttgaaat tgaaccgttc
    17641 tactgtctat cggcatagaa ccagtggact agccttaatt aaaaagttta tggaggaatt
    17701 tgaagaatga aaacacaata tcctatgatt ccctttcctc tcattgtaaa ggcaacagat
    17761 ggcgataccg aagcgattaa ccagattcta catcattaca gagggtacat aacgaagcgt
    17821 tccctacgac ttatgaaaga tgaatatggc aatcaaagta tggtcgttga tgaagtctta
    17881 cgtggaagaa tggaaaccag actgattaca aagattttgt catttgaaat taagtaatat
    17941 cctctctcct ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca gggaggtttg
    18001 ttattccacc aaagcatatt gagctttcaa tgtgttttga taggctaacg agccattgtt
    18061 ctttgaaaac tgaataaaag taatcgaata cgtttcgata agaaaagagc caacggaact
    18121 aaccgccatg acctatctta taaagatagc gagcgattca tgttagtgat ccgagaagca
    18181 atctttagca ggattgcctg caacgacatt cttatcgtga taatgatact cccatacagt
    18241 caatagtccg agcgtgataa aaccgtcgca ggcaatgagt atggctacat gagaaccatg
    18301 caggggtgga actcccgtga gctttgctaa agctgttcga ttgctggtaa aacaactttt
    18361 atgaaataca aataagtgat ttggaaagga ggattttatg aagcagactg acattcctat
    18421 ttgggaacgt tataccctaa ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa
    18481 caagctacga cgcttggcag aggaaaataa aaatgcaaat tggctgatta tgaatggcaa
    18541 tcgtattcag attaaacgaa aacaatttga aaaaattata gatacattgg acgcaatcta
    18601 gcgtcgccaa agggtcttgt atatgataaa atagtattaa gtcgtatcaa ggctctttcc
    18661 ataaaggaaa ggagcaaatg ccatgtcaga aaaaagacgt gacaataaag gtcgaatctt
    18721 aaagactgga gagagccaac gaaaagacgg aagatactta tacaaatata tagattcatt
    18781 tggagaaccg caatttgttt actcgtggaa acttgtggct acagaccgag taccagcagg
    18841 aaagcgtgat tgtatctcac ttagagagaa aatcgcagag ttacagaaag acattcatga
    18901 tggtattgat gttgtaggaa agaaaatgac actctgccag ctttacgcaa aacagaacgc
    18961 tcaaagacca aaggttagaa aaaacactga aactggacgc aaatatctta tggatatttt
    19021 gaagaaagac aagttaggtg taagaagtat tgacagtatt aagccatcag acgctaaaga
    19081 atgggctatt agaatgagtg aaaatggtta tgcttatcaa accatcaata actacaaacg
    19141 ttctttaaag gcttcattct atattgctat acaagatgat tgtgttcgga agaatccatt
    19201 tgactttcaa ctgaaagcag ttcttgatga tgatactgtc cctaagaccg tactaacaga
    19261 agaacaggaa gaaaaactgt tagcctttgc aaaagctgat aaaacctaca gcaaaaatta
    19321 tgatgaaatt ctgatactct taaaaacagg tcttcgtatt tcagagtttg gtggtttgac
    19381 acttccagat ttagattttg agaatcgtct tgtcaatata gaccatcagc tattgagaga
    19441 tactgaaatt gggtactaca ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc
    19501 tatggttgaa gaagcctatc aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa
    19561 gcgtgttgag attgatggat atagtgattt cctctttctt aatagaaaga actatccaaa
    19621 agtggcaagt gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa
    19681 cgaggataaa ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta
    19741 tgcaaatgca ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc
    19801 catgacgctg aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg
    19861 cttgaataaa gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta
    19921 cttatttacc acttctgaca gctaagacat gaggaaatat gcaaagaaac gtgaagtatc
    19981 ttcctacagt aaaaatactc gaaagcacat agaataaggc tttacgagca tttaagaaaa
    20041 tataaaaaga taattagaaa tttatacttt gttttactta aaaataaatg ttctttttca
    20101 tttattgatc cactgcgatt aatttcatca tctttattat gggttataca gtaagtgatt
    20161 tcatcattat ctgaaaaaat aattttcaca gtactacgcc aatcacctgg cattgttgtt
    20221 gttattggat tggaaatttg aatctcctta atatctgaat atgaattttt caatgctttg
    20281 actagactaa cctcatattc tcgattccgt tttagttctt cttttctttg ttgttctttc
    20341 atataaaatc ctccgctaaa aataagtagt aatagggcaa ctattagtag tattatttta
    20401 gaactctttt gcattttcac tcctttatga aacaattaca tcatctagct ataatatatc
    20461 aacttaggaa tttgaataag ccaatatatg aaaataccat tattcaaatt tctcctgacc
    20521 atctgaaaaa ataattttta tagttttttt tgttttacca attcttgaat caaaaaaatt
    20581 catatttttt tcattaaat
//
LOCUS       DATRNX010000029        20363 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRNX010000029 DATRNX010000000
VERSION     DATRNX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            312..1241
                     /locus_tag="VBC81_000856"
     CDS             312..1241
                     /locus_tag="VBC81_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6911911.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDFFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1298..2830)
                     /locus_tag="VBC81_000857"
     CDS             complement(1298..2830)
                     /locus_tag="VBC81_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6911912.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2939..4012)
                     /locus_tag="VBC81_000858"
     CDS             complement(2939..4012)
                     /locus_tag="VBC81_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6911913.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(4005..4781)
                     /locus_tag="VBC81_000859"
     CDS             complement(4005..4781)
                     /locus_tag="VBC81_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911914.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4778..5572)
                     /locus_tag="VBC81_000860"
     CDS             complement(4778..5572)
                     /locus_tag="VBC81_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911915.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5556..6710)
                     /locus_tag="VBC81_000861"
     CDS             complement(5556..6710)
                     /locus_tag="VBC81_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911916.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6759..7661)
                     /gene="murB"
                     /locus_tag="VBC81_000862"
     CDS             complement(6759..7661)
                     /gene="murB"
                     /locus_tag="VBC81_000862"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6911917.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7805..8293)
                     /gene="folK"
                     /locus_tag="VBC81_000863"
     CDS             complement(7805..8293)
                     /gene="folK"
                     /locus_tag="VBC81_000863"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6911918.1"
                     /translation="MTTVYLSLGSNISDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(8290..8652)
                     /gene="folB"
                     /locus_tag="VBC81_000864"
     CDS             complement(8290..8652)
                     /gene="folB"
                     /locus_tag="VBC81_000864"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6911919.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(8654..9457)
                     /gene="folP"
                     /locus_tag="VBC81_000865"
     CDS             complement(8654..9457)
                     /gene="folP"
                     /locus_tag="VBC81_000865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6911920.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(9461..10024)
                     /gene="folE"
                     /locus_tag="VBC81_000866"
     CDS             complement(9461..10024)
                     /gene="folE"
                     /locus_tag="VBC81_000866"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6911921.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(10043..11305)
                     /locus_tag="VBC81_000867"
     CDS             complement(10043..11305)
                     /locus_tag="VBC81_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6911922.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQIPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(11307..12194)
                     /gene="rarD"
                     /locus_tag="VBC81_000868"
     CDS             complement(11307..12194)
                     /gene="rarD"
                     /locus_tag="VBC81_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6911923.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(12181..13047)
                     /locus_tag="VBC81_000869"
     CDS             complement(12181..13047)
                     /locus_tag="VBC81_000869"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6911924.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(13049..14332)
                     /locus_tag="VBC81_000870"
     CDS             complement(13049..14332)
                     /locus_tag="VBC81_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6911925.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            14509..15397
                     /locus_tag="VBC81_000871"
                     /pseudo
     CDS             14509..15397
                     /locus_tag="VBC81_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(15420..16967)
                     /locus_tag="VBC81_000872"
                     /pseudo
     CDS             complement(15420..16967)
                     /locus_tag="VBC81_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(17110..18486)
                     /locus_tag="VBC81_000873"
     CDS             complement(17110..18486)
                     /locus_tag="VBC81_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6911926.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(18560..19567)
                     /locus_tag="VBC81_000874"
     CDS             complement(18560..19567)
                     /locus_tag="VBC81_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6911927.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(19675..20361)
                     /locus_tag="VBC81_000875"
     CDS             complement(19675..20361)
                     /locus_tag="VBC81_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911928.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6914 a         4072 c         3039 g         6338 t
ORIGIN      
        1 ttttttttca catagttatt ctgttttgaa aaaataaata attgagtata ttaataaatt
       61 tggtcatatt aatacaacaa aaaatcctta catgagataa ggagattaaa cgaccctgta
      121 aggattttga agttattaga aacactctaa gcaaaaattt taaagcgttt tctctatgac
      181 ttatattata tgtcatgttc ctatacaaaa cttacactat cctttcaaaa aataatacta
      241 tcctacgaaa cccatttttt gatagttttc tgttataata atactatcct acgattttag
      301 aaagaaaaac gatgaactca cttgataatt tactatcaca taatttagaa gataaccggc
      361 atttattacc ctatgaacac atgcatactg aggttagaaa tggttaccct gacattttat
      421 ttcactggca tccggaactt gaaatttcat atgtccacga ggggaccgca cgttatcaca
      481 tcgattatga tttttttaat agccaatctg gtgatattat cttgatccgt ccaaatggta
      541 tgcattctat tcatccgatt gaaaataaag agcatatcac agatagcatt aaatttcact
      601 tagatttaat aggatattct attgtagacc aagttagcct tcgttattta caaccgttgc
      661 agactagctc ttttaagttt attcaatgta tcaaacctag tatgacagga tataatgata
      721 tcaaaaattg tttgtttgat ttttttaata tttcaaaaga agaaaatcga cattttgagc
      781 tactcctaaa agctaaatta aacgaactct tgtatttact ctattatcat caatatgtca
      841 ttaaaaaaca tactgatgat acctacagaa aaaacgagag aattcgtgat ctgattgatt
      901 atattaataa taattaccag caaaatctta ccattgaatt tttagctgat tatatggggt
      961 atagtaagac acattttatg accgtattta aacagcatac tggaacatcc tgtacagaat
     1021 ttatcataca agttcgtctt aataaagcta gtgagcatct tattaactcc actacggcca
     1081 ttattgatat tgccaacagc gttggtttta ataatctatc aaattttaat cgccaattta
     1141 aacgctatta tcatacaact cctcgccagt accgcaaaca atttaccaac aaaaaagacg
     1201 gtagtacgct tgtctataga aatataaata caaaaaaata gaaactagtt aaatccatga
     1261 ctagtttcta ttttttatta tttaagcgtt aattagttta catcagctgc tcacgtactt
     1321 ctttaatttc cgattcattc acaactaaaa aaatggtgtc gccagcattc aagatagtat
     1381 ttcctgatac tacagccgat gtcttatgat gaatttgagt cgttattagc acattttctg
     1441 gtaattctaa atctcggaca tactttcctg atattttatc tgaaacagtt aattcaatca
     1501 atgtaggtgt cattacttta tcttttggat tgaaggtcat tttagccaac atcgcttcat
     1561 aaataggttc tccctttaat aaatccatga caatataaga taccattgtt acaattccaa
     1621 tagccattaa ttgttttaaa tcgccaacca tttctgtaac caaaatcata gctgttagtg
     1681 gtgcttttga gattgcacca aaatagcccg ccattcctag aacaacaaat aaagatatct
     1741 gctgatgaga tattatacca aagtactgca tacctactgc aaaaaaagca cctgctaaag
     1801 atcctaaagc tagaattggt aaaaaaattc ctcctggcaa gccactactg taactcaaca
     1861 tactccataa aaaacgaatg aggaaaaata atcctaacat catcagacta agattactcc
     1921 taggcagtga tacaattaag ccatttccac cccccaataa ttgtgggaaa tagtaaccaa
     1981 taggaagaat aaaaattacc gctaatatac catataggtg agatggtaga tgaaatagtt
     2041 tacctaaata atcatagatc acatgaaatc tcaaaattac ccactcatag ataaagccta
     2101 agatacccaa aaagagaccc atgagtaaaa aaatccaata aaagttgaga tttaaagatg
     2161 gtaattctga tggtaaagct aaaactgggg ttaaaccaaa gatattaagt gagacaaagt
     2221 ttgccactaa acttgctacc aaagctgtta tccaaactaa gcgagaaaaa tgatggtaaa
     2281 tttcttcaac tacaaataaa aggcctgcaa taggagcatt gaaggctgct gaaagacctg
     2341 ctgctgcacc actagcaatt aaaacacgtt tttccattcg actagcattg agatactgtg
     2401 caattccctt acctgtcata gctcctaact gaatactggg accttcgcga ccaagcataa
     2461 gtcccgaaga aatagctaaa atacccgcaa tgaatttctt ccatacaata ctaaaccagt
     2521 ctggcgatag cattccttta agttcacctt caacatgcgg gatacctgaa cctttaatat
     2581 caggatcaga tttgataaaa aaacctatta tcactgcagc gataatacta gtaaccgtaa
     2641 tgctcaataa aataattggt tgataatggg cacttttata aagttccata acaactaaaa
     2701 aaattttttc aatcgctaat cgaaatatgc tgacaacaaa tcctgctatt aagcctacta
     2761 ctattcctct ccaaacaaat cccagaattg attcaagtga aaactgaaat tcatttttat
     2821 gattttccat attttcctcc ttttcttaca attattttaa caaaaatatc acaaactttc
     2881 taggaactct tggtaactaa aaatacctct catcagagaa gtacttttag ttaatagatt
     2941 acttacgata cattttaaat tgtaagtaca aatcattata aataccaagc catttttgac
     3001 ccaagttatt ataaacttct aaatgatcaa ttgttttatc aggaggataa aatgctttat
     3061 catttttgat atctgctggt aacagcgctt ttgctttgag attaggggtc gcataaccaa
     3121 tatattctgc attttgtgcc gcatttttag gttccatcat gaaattaata aaggcataag
     3181 cttctttgcg atgctttact gttttgggga ttacaatatt atcaaaccaa aggttcgatc
     3241 cttcactagg aacaacgtaa tgcaaatgct tgttcccatc aagcatttct cgtgcttctc
     3301 cagagaatgt aacaccaatc gctgcatctc cctgaatcat atagcctttc atctcatcag
     3361 caacaatagc cttaacgttt ggtgttagag catctaactt tttagaagcg gcttttaatt
     3421 ctgatatgtt ctttgtattc aatccatatc ccaaggaatt taaaccaact ccaataacct
     3481 cacgtgcact atcaactagc atgattttat ttcgaaactc tggacgccat aaatcatccc
     3541 aatgtttagg aggagtttta actaattgat cattataaac aatcccaaca gttccccaaa
     3601 aataaggaat cgaatattta tttttaggat cgaaactcaa atttttaaac ctagctccaa
     3661 tagcatccca attagctatc tttgaatgat caagtttaac cagaaggttt tcttttatca
     3721 ttttgtcaat catataatca ctaggtacag caatatcata tgtcgtccca ccctgcttaa
     3781 ttttggtgtg catagcctca ttagagtcaa acgtttcgta ttgtacttca attccagttt
     3841 cttttgtaaa tttttttaat aaggctggat caatatagtc tccccagtta taaataacta
     3901 acttatctga gttaggtgtt gaactactct tcttttctaa gtaagtggtt aaaccaaaga
     3961 ggatcaggat aacaagaata ataccgccta aaaaggaata aactctacgc attcttttct
     4021 cccttctctt tcgaaatata gtaataacca attaccaata aaatactaaa taaaaagaca
     4081 atagttgaca aggcattaat ttccaatgat ataccgcgac gtgcgcgtga atagatttct
     4141 actgaaagcg ttgaaaagcc attacctgta acaaagaaag taaccgcaaa atcatctagc
     4201 gagtaagtga aagccataaa gaaacctgaa ataatacccg acgacaaata gggtaacata
     4261 acttctttaa gcatttgcca agttgaagct cctaaatcat aggaagcatt aatcatatca
     4321 tcattcattt cttttaaacg aggtaagacc attaaaacaa caataggaat agagaatgca
     4381 acatggctca ataatacaga agtgaaacct aactgtaaac caataacagt aaatactaag
     4441 agaaaacttg caccaatcat aacatctggt gctactaaaa ggatattatt aagcgataaa
     4501 atagaggttt gatgtctacg tcttacctga taaatccaaa tagctccaaa ggtaccaata
     4561 attgtagcta ataaggcgct aagaaaagcc aaaaagaatg tttgcactaa aattagcatt
     4621 aagcggctat cctgaaacag ttctccatag tgactaaagg tgaatccagt aaaagaattc
     4681 atatccccac ctttattgaa agaataaaaa atcaaataaa taataggaat ataaaggata
     4741 ataaaaacaa atgctaaata gatatttgcg aattttttca tgctttcctc tcctttgtta
     4801 accacataat agcaaccatc actaaaatta aaataacccc tatggttgag cccatacctt
     4861 tattttgtgt gattaaaaaa tgttgttcta tggctgtccc taaagtaata actcgattac
     4921 caccaataag acgcgtaagc ataaagagac ttaaactagg aataaagaca gattgtacac
     4981 ctgctcttac accattcaga gatagaggaa atataacctt ctgaaaagtt tgccatgcat
     5041 ttgcacccaa gtcatcactt gcataaatga gattttgatc aatatcatcc aaggcattaa
     5101 aaatgggtaa taacataaac ggtaattcaa tgtaagctgc tacaaaaaga aatgaaaaat
     5161 ctgtaaatag aatctgtttt gggccgatac ctataaaggt taagaaggcg ttaattcccc
     5221 cttgttgacc aaatatcccc ataaaagcat aggcttttaa caaaagatta atccatgttg
     5281 gcaaaataat tagcattagc cataattgct tgtgttttaa ttttgtaagc aaataagcag
     5341 ctggatagct aatcaaaagc gttatgaaac taacgatgcc tgcatatagg accgagttaa
     5401 agctcatttt aagataggtt cctgagctaa aaaacgtctg gtaattagct aaagtaaagt
     5461 gcccattaat atcaaagaac gagttccaag caatcaaagc aacaggtgct aatacaaata
     5521 agaataacca agccatataa ggaattgaaa atagactaga ggttttcttc atttctctcc
     5581 tcctcgatag cattgattaa accatcttca tgatcgtcag tgtctacata ttcttcaatg
     5641 cgcgcatcaa attcttcttc tgtttcatta agacgcataa tatgtatatc ttctggagtg
     5701 aaatctaacc caataacttc accctcaata gctttacgag ttgaatgaat catccattca
     5761 ttacctaaat catcataagc aataatttca tagtggacac ctcgaaaaag ttgtgtgtcc
     5821 actttaactt gaagttttcc ttcatcgggt agggtaattt gaagatcttc tggacgaata
     5881 actacttgga ctgattcatt agggcgcata ccgccatcaa cagcctcaaa gcgcttaccg
     5941 ttaaattcaa ccaaataatc ctcaatcatc ttgccagata agatatttga ttcacctata
     6001 aaagttgcta caaaatgatt gataggttca tcgtagatat ctactggtgt tcctgattga
     6061 acaatttcac cttcattcat gacgaaaatc caatcactca ttgccaacgc ttcttcttga
     6121 tcatgtgtga caaaaacaaa ggtaattcct aaacgttgtt gcagttcgcg caattcatat
     6181 tgcatctctg tacgcaattt caaatccaat gctgataagg gttcatctag taagactact
     6241 ttaggttgat tgatgatagc acgcgctatc gcaacacgct ggcgttgtcc tcctgataat
     6301 ttttgaatag ctcgcttttc aaacccttct aaacgaacca tttttagagt ttcttgaaca
     6361 cgtttttgaa tttctttctt atccattttt ttcaacttta atggaaaagc aacgttttca
     6421 aaaaccgtca tatgtggaaa caaagcatag ttttggaaaa cggtatgaac atcccgttta
     6481 ttagtgggaa catcattgat gcgtttccca tctaagtaaa tatcgcctgt actcgcctct
     6541 agcaaacctg caataatatt taagatagtt gatttaccac ttcctgaggc ccctaataag
     6601 gtgtaaaatt taccttcttc aagttcaaaa ttaatatctt ttaagacaac cgtgttgctg
     6661 tcttcgaata cctttgatac atttttaaat gcaatgattg ggttagtcaa ttctcataaa
     6721 tcctccattt ttctgactaa agtaagtact aaactacttt ataatagcga ctcaccgata
     6781 atacgaactt ctggttccaa atggacacct gaagttgatt caacggtgtt aataacatgt
     6841 tcaataagat tttcataatc ttgagctgaa ccttctgcaa tgttaaccat aaaaccagca
     6901 tgtttttgac taacctctac tccaccaata cgttgacctt taagatgtgc ttcgctaatg
     6961 agttgccctg caaaatgtcc tggaggacgt ttgaacacag aaccacaaga agggtattct
     7021 aaaggttgtt tcagctcacg gagataggtt aaacgatcca tttcctgagt aatcatgaga
     7081 tgatctcctg gttttaaggc aaacttagct gatataacaa tatctccact ctcttgaatg
     7141 acagaatgac gataaccaaa ttgcatattc ctagcttcaa tcgttttgag ttcaccctgt
     7201 ggtgtcaaaa cttgtgcaga caataaaata tgagcaattt caccaccgta agcgccagca
     7261 ttcataaaga ctgctccacc tacacttcca ggaattccgc aagcaaactc aaagccagtt
     7321 aaactatgat aacgtgcaat acgagttgtt tcaatgagat tagctcctgc ttctgcttca
     7381 ataacataac cgttgactgt aacagttgac agtttatcaa acatgataac aaaaccacgg
     7441 ataccaccat ctctaacgat aatattactc gcatttccta aaaccatcca aggaatattt
     7501 tgactgttag caaacttaac aatacgcgat agttcaaggc gattccttgg gaatgcaaga
     7561 taatcagcag gtcctcctac tttagtataa gtatatttct ttaaaggctc atcaaagcga
     7621 atatcgagac cttctaattc tttttgtatt gttttaatca taacctttat ctcaattctg
     7681 ttttattgtg ttttatagac acaaaaaatc ggcataaaac cgatttctat tataccaaaa
     7741 atataggttg ttgacgatac attatcacaa tttctgtgta ttttaatttt aaaatcctaa
     7801 ctgactattc gactggtttt aaataatgaa cttctcctaa attcaatttt tcgagatagt
     7861 cttctagctt agaaccatta ggtaaactta gttgaggtgc tatttcgagt aacggaacta
     7921 gaacaaatgc tcgcttcgtc atatacggat ggggtactac taaatcttct tggttaatca
     7981 cttgattacc aaaaagtaag atatcaatat ctattgtcct tggaccccag tgctcatgtc
     8041 tcacgcgctt taagcgtttt tcaatttctt gacaataatc caataattca aaaggtgcta
     8101 agtctgtctc tacctgacaa catatattaa aaaaatcttc ttggttagta ttcccccaag
     8161 cagctgtttc ataaatagcg gaaatttgcg cgacttttgt cttttgcaaa tgatcaattg
     8221 aaaagagagc ctgcttcaga aaagtttctc tatcactaat attactacct aaactcaagt
     8281 aaacggttgt catactcgct tgcgctccaa ttcaattcca actgattcat agtgcccatt
     8341 aattggtgga ttgtcttttg taatctttaa tgtaatagct tgtactggat gaaactgtaa
     8401 gaaaatatct tcaacaataa cacctgctaa tttctctatt aattgatact gttcgttttc
     8461 aacatgattt ttaatacagt caaaaacttc accataatga accgtatcaa ttaaatcatc
     8521 agtttgtgat gcttttgcta aatcaagtga tagaacagca tcaacttgaa aaacttgccc
     8581 caatgtttgt tcttccgaaa aagcaccatg ataaccataa aaccgacatt tatttaaata
     8641 aatcttatcc atattacacc aactgactaa ttactgtcac aatgtcctta tttacttcaa
     8701 cattgtgaac acgtacaatc tgacacccct ttgctattgc ccatgccgat aaagcagcag
     8761 tccccatatc acgttcagtt acttccctat taccacctag aagataatta acagtacgtt
     8821 ttcgagagat cccaaataac acaggataac ctaaatcaca cacttgttcc aaacctttta
     8881 atagctctaa attttgctcc tctgttttag caaaaccaaa gccaggatct atccaaatat
     8941 tatcctttga aacacctgct tctaaagcac gctctgctct ttcaagtaag aattcacaaa
     9001 cttctttttt gatatcttga taaacagctt cttcctggtt atgcatcaaa ataattggaa
     9061 catttcgttc tgctgctaaa gatagcattt taccatcata taaacctgac caaacgtcat
     9121 ttaaaatatg tacacctgca tcaagggctg cttgagctac ctctgtttta taagtatcta
     9181 ttgataacaa aagatcaggg tagacctctc taatagcttt aatcattgga actacacgtt
     9241 taatttcttc ttcagcagtc acaaatactg cacctggccg cgttgattct cccccaatat
     9301 ctacaattgc caccccttgt tcaagcatct caccaacttg atttaaagct gaatcaattg
     9361 tggtataaga accaccatct gagaaggaat caggcgttac atttaaaatc cccatgatac
     9421 aagctttacc tgtaatatca tattgtccta ttttcatctt ttatcctttt tgtattagcg
     9481 atagaatttc ttgtctttca tatcgatcgt ccttgtataa accacgagct accgttgtaa
     9541 tcgtcttact tcctggtttt ttaatgccac gcattgtcat gcacatatgc tctgcttcta
     9601 tcataacaaa aataccttta ggcgctaaag catcctctaa agcttgtgca acttgtgctg
     9661 ttaaacgttc ctgaagttga ggacgcttac tcgccacttc aactgcacgt gcaagcttac
     9721 taagtccagt aacacgtcca tcgtttggta aataagcaat atgagcttta ccataaaaag
     9781 gaaccaaatg atgttcacac atggaataga atggaatatc cttgacaatc acaacctctt
     9841 catgattttc agaaaagact gcagtgaatt gctcctttgg atcttgatta agtcctgaga
     9901 acatttctat atacattttt gccacacgct tcggtgtatc tttcaaccct tctcgatcag
     9961 gattttctcc taaagcctct aaaaattgat aaatagcttt ttccattttt tcttgattgg
    10021 gcattaattt atcctctcct ttttaagttg ttgtagtttt gattaatagt agttcctgac
    10081 gaacttgaga aataaaataa agggagccag taatcacata gaaatcttta tctgatgtca
    10141 gatttatatc atgtagccat ttcttccaat cactaactct agtgtacgct ttaggatatt
    10201 tgtctaagtt tattgattta gggtaatcaa agctagtaac cgaaaccgga gcgatactag
    10261 acaatgattc taacatactc tctatcggtt tagtattaat tgcagcaaat agaatatgca
    10321 cgtttttctg ctgatatttt tttataactt gaacgagagc atcgacactt tcattattat
    10381 gagcgccatc aatcattaca tttggaaaga ttaattctgt acggcctacc cattttgtca
    10441 tttcaagccc atctttaatg gtctgtaggg ttaactttgg atatttgtct cttaataata
    10501 aactggtagt tatagctatg ctagcattgg aaacttggtg atgtcccgga atttgcaact
    10561 gaatatgatc aatatttgct tgaggaccta tatagttaaa ataattttcc tcttctttta
    10621 tataaaagtc attgtgtaaa cgataagtct ggctgtgggt ttccttggct tttatttgaa
    10681 agacttcttc aacatcctga cgatcattag catatacgaa tggaactcct ttttttaaaa
    10741 cacctacttt ttgttcagct atatctacat aagttcgtcc taaaacctcc tgatgatcta
    10801 atccaattga cggacaagta accgctaaag ccttgaacat gttggtagaa tcataataac
    10861 cgcccattcc agcctcaata atgacgatat caactggaca gctattgcca aaataataga
    10921 acatcaaaac tgttataact tcaaattctg tagctggttc taggttggtc tcaaggtgca
    10981 accgttcaac aactggacga acagtctcaa caagtttgat aaaatcagac tctggaatca
    11041 tttgaccatc aatgctaata cgctctctaa agtcaacaat atatggtgat gtaaatgttc
    11101 caacttgata accagaattt gtaaaaatat gttgtaaata actagttgtt gatcctttac
    11161 cattcgttcc aacaacatga atggcagata ggttgttttg agggtttcct aactgctcta
    11221 gcatccatct catacgttcc aaacctggtt taataccaaa agctaatttg ctatgaatcc
    11281 attctagcgc ttcttgataa gtcatcttat ttacctttct ttaacatcgt atgtacttgt
    11341 ccaatactaa aaactaaaat agcaagccag ataaaaataa aacctattac ctccccacta
    11401 acgatagttt ctttaaaaat aaacaatgct agcaataatt ggatggttgg attgatgtat
    11461 tggataaaac cgataatatt tagaggagcc ctcttaatgg cttctgcaaa taataaaaga
    11521 ggaactgctg taatgattcc tgaaagtgag agtaaaacta attgtaatat attataatca
    11581 gttaaaaaat ctttggcgaa aaacactata taaatgagtg caaacggagc aataaaacta
    11641 ctttctacca gcatcgaaaa atcgctagat aaagaaattg atttttttaa taacccataa
    11701 aaaccaaaag aaagcgctag agttagtgaa attaaaggaa aatgaccagt ttgataaact
    11761 aaaattccaa ctcccatgat tgcaatcaaa attgcaaggc taactactct tgccaggtgt
    11821 tcacgtaaaa caaggaccga caacaaaatt gaaataatcg gcatcatata ataaccaaga
    11881 cttgcctcag tagcgtgacc atgcgtgact gcaaaaatat agaccaacca attaattgaa
    11941 attaaaatac tggctacaaa cattgtccaa aatgactttt tattattaac taagcctttt
    12001 aagtctttta aatagactgt ttttctaccc gaaacgagca tataaataag catagttaaa
    12061 acagtgaata taatcctata agcaaacgta ctatatgctt caattccaga taataatttc
    12121 caataaagag ataaaagccc ccataaaaca taggctgaca agccaaggat aattcctaaa
    12181 ttatcttttc tcaactctca ctccttttcg ctcaatatta agtacttgaa tctgtccatc
    12241 taaacataat ttacttaact ctgtgacaat tggttgttct ttttctttcg aacacagaac
    12301 cattaccgtc ggtcctgctc cagacaaata agtagcatac gcaccgtttt gagtgctgat
    12361 ttgttttata actccaaatt ccttcaccaa aggttgacga tatctttcat gaaagaggtc
    12421 cctttctata gcccatccag cagttaccaa atcacctttc aacaacgacg caaccatcac
    12481 attagcgacg ctgcttgaag caactgcatc ctcataactc aatttttgag gtagtacatt
    12541 tcgactatca cttgttttta attcataatt tggaataaaa gcgataaagg aactatctgg
    12601 aaacatcact tccaaacttt taatatcatt tttcactatt gatgaaataa ctaaatcacc
    12661 aaaaatggcc ggtgctacat tatcaggatg tccttctaat cgggtagcta tctctaattt
    12721 ttctttttga gaaagagcca aatttcctaa ttgattggct aattctattc ctgcaacaat
    12781 tacagaggaa gaagaaccta aacctctggc caaaggaata tctgaaaaca tttttaagcg
    12841 gtgtggtgcc aaatccgact taacatgcaa agctgtttga ataaggaggt tagtatgatc
    12901 ttttggaata tttactaaat tgtgttcaac taaccactct gtactttctt ctaatacttc
    12961 aatgcttaaa tactttgata acgcaactcc tattgaatca aagcctggac caatattcgc
    13021 actagttgca ggtacaatta tcctcatttc accctcctat tactttgaaa acattgatta
    13081 actgaatgac accactctca ggaaactcct caataaattg agttattttc tcttggctaa
    13141 cttcatctgt taatacatag atttcttgtt gtttatcatc caatgctttt tcaataacat
    13201 ttttcaaacc tatattttct tgctcaaagt accttccaag tgtatcacta tcttcagcat
    13261 ttctcaaaga aatagctaga tagtactcat tgaagatatc agactgcatt gacaacctac
    13321 acggacaatg ataagtattc ataggcttaa tttttatctg tgatcgaata ctacgactaa
    13381 tatcgatgat atcggctaaa acagaggttg ctgttggatt ttgtcccgca cctggtccat
    13441 aaaaaagaga ctgaccaatc cctattgatt caatgaatac tgcattcatc acatgattaa
    13501 ctgcagctaa gggatgctct tgagaaatta gtgtcggaga aacgtctaca cttataccag
    13561 ttggagtttc acgaagatca gttaccaact taatggcaaa gcccatctcc tgagctactt
    13621 caacgtcctc tgagcgaata ctagaaatcc ccttataatt aacatcatca aaatcaatag
    13681 gcataccaaa tccaaattga cttaagatag tggctttgta ggcagtatca ataccttcaa
    13741 catcatttgt aggatcactt tcggcataac ctaactcttg tgcctttttt agagcttgtt
    13801 cataagacca tccctcttca aacatttttg ttaacatgaa gttggaggta ccgttgagaa
    13861 ttcctaataa acgtgtcatt ttatctgagg caaacgaatg cgatagggtc cttaaaatag
    13921 gaatgcctcc agcaactgct ccctcataga ataaagctag acctctagcg tctgctaaat
    13981 ccatcaactc ttttccgtgt aaagcaatga gatctttatt ggctgtcaca atatgtttac
    14041 caaatcctaa tgcttgactc aaataagttt tagctggctc aatccccccc attaattcca
    14101 ctacaatatc aatttgtgga tcttgaagaa tagtatttat ctctgtcaca aagttataag
    14161 taaatccctg attgatgaaa cgctggcggc tttcattatc tcttactaat actttatcaa
    14221 ttacaatatc ttcgccttct aaagaaagta gcttatgttg attttctttt agcaaatatg
    14281 gaataccctt agcaaccgtt ccaaaaccta ataaagctat tttaatagtc atgaatttct
    14341 ccttagaaat tgtctctaac ttcaagacaa acattggcta tattataaca aattaccaat
    14401 attttttata gggtaaacgc gtcttgtcct atgaaagttc ttaaaaagag aatattattt
    14461 cagaaaagat gatacaataa aagtacttta aaagttaagg agaaataaat ggcacacaca
    14521 cctactagtc atcgtaaacc acgaaaacgt tccccatggt tagctattgc ttctgtcttt
    14581 tttttgttaa tagctttaat cggcatattt ctattcttca acaatcgttc aaagcaagaa
    14641 ataaaaacaa aaacgaacgc ctctagtcat cgaaaaattg tcacatctat caaaaaaaag
    14701 aaatgggtta agcaaaagac tcctgttaaa atacccattt taatgtacca tgctgttcat
    14761 gttatggacc cttctgaagc cgccagtgct aacttaattg tggcacctga catttttgaa
    14821 agtcatataa aacgtctcaa aaaggaagga tattacttct tagcccctaa cgaggcatat
    14881 cgtgcactaa acgaaaatgc tttacctgaa aaaaaaagtc atttggatta cttttgatga
    14941 tggaaatgcc gatttctaca ctaaggcata ccctatactc aaaaagtaca aggttaaagc
    15001 cactaataat atcatcactg gctttgttca agaaggacga gaatctaacc tcaacgttca
    15061 acaaatgcta gaaatgaagc aaaacggcat gtcattccaa ggacatacgg ttactcaccc
    15121 caatctttct ctcttaactc cagaactaca aacccaagaa atgacactat caaaactgtt
    15181 tttagatcaa aaactctctc aagatactct tgcgattgct tatccatcag gacgctacaa
    15241 tcctacaact ttggatattg ctagtcaata ttataaacta gggcttacaa caaacgaaag
    15301 agttgcaaca aaagataacg gtttgctttc tcttaatcga gttcgtatct taccaactac
    15361 tagcgatgat gatttgatca aaacgattaa ccaataaaaa gaagccttct ggcttctttt
    15421 tattcttcat aagatttaaa tggttcattt ttacgtggat aacgatctgg agaaatcgat
    15481 aagcccattt cttttttctc atggaaaagc tccactaata gcgctagcat catcaaaatc
    15541 attacgaatg aaaatggcag agcgacaata attaaaacgt tctgcagcgc taacagacca
    15601 ccagacaaga gtaagacaat agcaatcact gccaacacta gcccccaaat tactttagtt
    15661 cgattttttg gatttaaatt accatcttct gtcaacatag ccaaaacgta cgttgcagaa
    15721 tctgctgatg taataaaaaa tgaaaaaatt aaaatgatag caatagtgga taaaagccaa
    15781 cctaaagtgt agtgattaaa agtagcaaac aatacttcct ctgttgcaaa ctttgtcaaa
    15841 ttaataccca gttgttgtac ctgagttgat aatgtgccaa atgctgcaaa ccatacaaaa
    15901 ctcaataatg tcggtattaa aagaactacc gttaaaaact cccgaatgct acgcccttta
    15961 gaaatacgag cgatgaaaac tccaacaaaa ggagaccagg aaatccacca cgcccaataa
    16021 aaaatcgtcc aattatcaat ccaagaacgt ttagtattgt caaaagcagc tgcacgaaag
    16081 ctcattccaa agaaattttg taaataagag cctaaagact ctgttagggt atcaaaaata
    16141 cgaaccgttg gtcccaatac aataactaaa gctaagaggg ctaccgctaa aatcaaattt
    16201 aagttcgata aaattttaac accttttcct aaacctgata aagctgacat aacaaataaa
    16261 gctgttgtaa taaggataat aacaatttga acaaatgcat tattgggaac acccaataaa
    16321 aaacttaacc ctccgttgat ttgagcagct ccaaatccaa gtgtcgtagc aactccaata
    16381 actgtagcaa caactgtggt gatatcaaca atttttccta gtcaaccatc tgtcttatca
    16441 ccgaaaagag gctttaaagt gacagacaag aggtatttct cttgctttcg aaatccaaaa
    16501 taagctagag ccaaagcaac caatgcatat actgcccaag catgtatccc ccagtgaaaa
    16561 aatgtaaaac gaaatgcatc agctaatgct gtttgcgatt ccttaggtgc gccaggtgtc
    16621 gaaatagcaa aatgcgataa ggggtcagct gctccgtaga agaccaaacc aatccccata
    16681 cctgctgaaa acatcattgc tatccatgat acagttgaat attcaggacg tgaatgaggt
    16741 cgccctaaac gtatttgtcc aagaggactc ataataaaaa agacacacaa agcaagaatt
    16801 gaagtaacta acaacaagta caaccaaccg aaattggtag atacctgttc acgcaagatt
    16861 tgtgtccaag attgaaaacc acgaggaaca ataattccta ataagactaa aataagcgat
    16921 acaattagtg aacctgtaaa cacaggcgta atatgttttt tactcaaaat ttcctcctat
    16981 actaaaatag tatagcatac tttaataaat actcctctca ttttaatcac ctgtatcaag
    17041 acctctcctt gtctcctcaa caaaaaagaa ccagataaca ttatctggtt cttgtgtttg
    17101 acttagtgat taatcactat ttggtgtaaa taccaaatgt tcgttgacaa aggtatcaaa
    17161 tccaagttgg ctcaattcac gaccatatcc tgaattttta atacctccaa acggtaattc
    17221 cggtaatgat gtccaccctg aattaataaa tgtcatccct gtttcaatct gagccgccac
    17281 ttttttagca tgttctggat cagaagagaa aacagtactt cctaaaccat aactagaatc
    17341 attagctaaa gcaatagctt cttcttcagt atccacttta tagatagagg ctacggggcc
    17401 gaaaatttct tgattataga ttggatttgc tttagtgata ttcgttaaaa cagttggcat
    17461 gacgaagttt cctggatgat ctatagtgtc atttccaaaa actacctcag caccgtggtc
    17521 taccgccaat ttaatttgtt ttaaaacatc atcctttgca cctgcagatg acagtggagc
    17581 caaggttgtt tccgaatcca ttgggtcacc ccatttagca gacttaaagg tctcaactac
    17641 catatttaca aattcgtcat acttatcagc cataacaatg aaacgttttg aagatgtaca
    17701 tacttgacct gcattatata aacgcgcaaa gaatattgtt ttactaagta aatcaaaatc
    17761 agcatcatct aaaataagaa aagcatcgtt cccacctaat tccatagacg attttttcaa
    17821 atttttacca gcctcagctg ctattgatgc cccaccacgc tcagaaccag ttaaacacac
    17881 acctgcaaca cgaggatctg atataaggtt tgaaacttga tcatacgaag caaaaatatt
    17941 tttaaacgct ccttctggag ctcctgcttc tcggactaaa tcctcaaatg cttgagctga
    18001 tgcaggacaa atagaagcat gttttaaaag catagtatta cccactataa agttaggggc
    18061 aaatacacgc ataatctgat aaaatgggaa attccatggc tcgacagcaa gtaaaacacc
    18121 gacagcttgt ttcaaatagt aagcctctcc atttggactt tctaacggaa caggttttaa
    18181 aaacttttgc ccattatcag cataatagtc agctatgtct gcacacaagt ccacttctcc
    18241 ttgtgcttca gtgaaaagtt ttcccatatc tttagtcata acttcagcgt acttatcacg
    18301 gtccttacgt aataaatctg ctactttatg caattgattt tggcgttctt caacattatc
    18361 ttctttgcgc catgtcttgt agagggcatg cgctatgtct aatgactgtt cgagatcact
    18421 gtcactgata ttatcgaatt catgcaatac ttcgttagtg tacggataaa ttgttttata
    18481 tgccataatg ttccctcctt ttgatatcct cattataaca tttatgaaat cgattacaaa
    18541 tatttagctt tagacaagat caccaagttc caaggatagc ttgcattgtt aaactaacta
    18601 taataatgcc taaccaacac gctgcaccta aaagaattga ttttcctcca gttttaatta
    18661 gtttgctgac attagtattt ataccaattg cccccattgc cataacaata aaaaatttag
    18721 ataaaacctt aagtggctca aagatacgta acgaaaaacc taatgaagca actatcgttg
    18781 ttagcaaaga agctaagata aaataaagca caaacttcgg aaatatctta gtaacactta
    18841 cagattgttc tttctgcgtt tgtttcatat gataaataga taatacaatc gtaattggaa
    18901 tgatggctaa tgttcgtgtc aatttaacaa tagtagctcc gcccaaagta ttgctgtgat
    18961 tgatagcatc ccatgcagta gcagttgctg taactgacga ggtatcattg acagcagtcc
    19021 ctgcaaataa tgcaaaaccg tgatcagaga gaccaataaa attccccagc gttggaaaaa
    19081 ttaaagccgc taagatattg aataaaaaaa taaccgaaat agcttgtgct acttcatcat
    19141 ctttagcatt aataactggt gcagtcgctg caattgcgga tccaccacaa atagatgaac
    19201 caacacctat taaagtagca atcgttttat ctaacttaaa taacttttgc aaaacataag
    19261 caataatcaa tgaaatggaa atcgtcataa taatgatggg taacgatgat ataccaactt
    19321 tcatcacttg catgaggttt agtccaaaac ccaataagac aactgctgtt tgtaaaatat
    19381 atttagacgt aaaagcaata cccttattaa acaattgtcg attttgataa aaggatccaa
    19441 caattattcc aatcaagata gcaaagactg gtgctcctat taaagggaga taaagcccaa
    19501 gcaaccaaga tggtatagca ataataaagc ataatattag tccaggaatt ttttccttaa
    19561 ataacatgat agtcctcctc acattgacta ttatatcaca agctttaaaa cgtggcagga
    19621 aaaccgaaag aggaccagaa tatgctctga tcctctttca ttactaattc acaattattt
    19681 agttactttc ataaatggat atgatttttc gttacctaaa atcaattttg agccctcttt
    19741 tttataggct gttttttcac catttttcac aatattacca tagaacgcat ttgccttttt
    19801 attgatatgc gatactttaa taacacttgt taagcgatgt actttaccat ccataactgg
    19861 caatgtcatt tcaccagtat tagtattata ttttcctccg acagcttgtg cagcactttg
    19921 gaaacttcta tcaatttgtt ggtcaatttg tttgtcagtg atttttgttt catcaaaaat
    19981 ctttttacct gcttcactta aatcattgta agtcaaacct tgatctttta attgtttttc
    20041 aagagcttta tcaataaaag tttttatagc ttgatggaat tttacctcat caatttgagc
    20101 agtgactgtt atctttgcct catcgttttt gacgttcata tgcatattag cgcccttaac
    20161 caaatcagct gcactaatcc ccatatccgc tgcctgactt ccaagttgtt gttctatttc
    20221 tttctcaact tgcttctcta aagaaactgt acgccatttc ccctctagtg actgacgatt
    20281 attccagaaa tagagaccgc ctgcacctac aattagaata agtactagta cagttaaaaa
    20341 aacttttaaa aactttttca tga
//
LOCUS       DATRNX010000030        20361 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRNX010000030 DATRNX010000000
VERSION     DATRNX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(326..2908)
                     /locus_tag="VBC81_000876"
     CDS             complement(326..2908)
                     /locus_tag="VBC81_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6911929.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(2935..3858)
                     /locus_tag="VBC81_000877"
     CDS             complement(2935..3858)
                     /locus_tag="VBC81_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6911930.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            complement(4113..4556)
                     /locus_tag="VBC81_000878"
     CDS             complement(4113..4556)
                     /locus_tag="VBC81_000878"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6911931.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            complement(4566..6782)
                     /locus_tag="VBC81_000879"
     CDS             complement(4566..6782)
                     /locus_tag="VBC81_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6911932.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            7042..9444
                     /locus_tag="VBC81_000880"
     CDS             7042..9444
                     /locus_tag="VBC81_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6911933.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            9601..10056
                     /gene="nrdI"
                     /locus_tag="VBC81_000881"
     CDS             9601..10056
                     /gene="nrdI"
                     /locus_tag="VBC81_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6911934.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            10067..11104
                     /locus_tag="VBC81_000882"
     CDS             10067..11104
                     /locus_tag="VBC81_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6911935.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            11104..11601
                     /locus_tag="VBC81_000883"
     CDS             11104..11601
                     /locus_tag="VBC81_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6911936.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(11823..12860)
                     /locus_tag="VBC81_000884"
     CDS             complement(11823..12860)
                     /locus_tag="VBC81_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6911937.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(12872..13645)
                     /locus_tag="VBC81_000885"
     CDS             complement(12872..13645)
                     /locus_tag="VBC81_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6911938.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            complement(13649..15298)
                     /locus_tag="VBC81_000886"
     CDS             complement(13649..15298)
                     /locus_tag="VBC81_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6911939.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            complement(15436..16263)
                     /locus_tag="VBC81_000887"
     CDS             complement(15436..16263)
                     /locus_tag="VBC81_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6911940.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(16260..17069)
                     /locus_tag="VBC81_000888"
     CDS             complement(16260..17069)
                     /locus_tag="VBC81_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6911941.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(17073..17576)
                     /locus_tag="VBC81_000889"
     CDS             complement(17073..17576)
                     /locus_tag="VBC81_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6911942.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            complement(17594..18019)
                     /locus_tag="VBC81_000890"
     CDS             complement(17594..18019)
                     /locus_tag="VBC81_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6911943.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            complement(18266..19300)
                     /locus_tag="VBC81_000891"
     CDS             complement(18266..19300)
                     /locus_tag="VBC81_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6911944.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            complement(19529..20341)
                     /locus_tag="VBC81_000892"
     CDS             complement(19529..20341)
                     /locus_tag="VBC81_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO6911945.1"
                     /translation="MKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVS
                     NSTYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFI
                     LAYALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFV
                     GLLFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTD
                     TSALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
BASE COUNT         6605 a         3929 c         3317 g         6510 t
ORIGIN      
        1 tacagtagct tcaccaaaat tttaactaat aacaattaca tgtaataaaa caagtgagcc
       61 actactagaa acctgttata cagcttttag tagtggctct ttatttagtg attataactt
      121 tagtcgtttt ataaaagtca aaacaagaaa acttcctcaa acaaaaatac attaactctc
      181 ttttaagcgt aaaacaatgg ttgttcaaaa gaagaattag atatatcatt aaaatcttcg
      241 cacgctagaa atcataacta aaaaacaaac aagtgcggtg acttgtttgt ttttagttgt
      301 cagctaagtt tattcaactc ttttattatt tcacttctgc tagtgtttta atatcaacaa
      361 gcactttaga gccatctttt ttagtaactt gaacttgatt accattaata taattaaagc
      421 tttcaaaact tacactatat ttatttgata attggatgag ttgcttttca aaagattgtc
      481 tatccatacc atatttctta gcgttttgtg ccattcctag ttcatattca tctagctctg
      541 attcatcttc ttgtttttct ggttcttctt cgtctgaacc tttattaaca tcataaaaac
      601 catagttgtg agttactttc cctgaatctt caggttcttc atctggagca taatttttgg
      661 ctttattgtc tttatttgag cctttgttat gatcactgtc gtgtccccaa cccttttctt
      721 ttggtaattc attaggatgt tccatgtagt atttaattgt tgagaagagt tgttgtaatg
      781 tatagccttc tggtgctttc cataaaccac ccttgtcaaa ccatgccata ggaacattat
      841 gataatgatc atgatgagga acaatcaatt gatcatcttc aaatccaact gcatatgcca
      901 tgtgagcagc aattttatca agcggtacaa ttttttctgg ttttgcttgt ttgaatactt
      961 ctgcagcact taagtttttg tcactagaat ttgatgttga tttattatca gttggtgcag
     1021 gacttggttt atctgcttta ttagagtcct tttccttata aatttcagca tctttacccc
     1081 atccttcaac tttaggacgt ttttctgggt gcatcatgta atatttagcc gtagctaaat
     1141 agtcttctaa agaatatgtc ttatctgaat cagctaaaag atctttttct tcatcaaacc
     1201 attttaattc cacataatgg taatgatctt tatgaggaat tacaaatgta ccattcttat
     1261 agtctgtcgc ttgtgcaatt ccaaataata aatcttcagg tttaacaatt gcttttggtt
     1321 caattgcctc atagatttct tgagctgttt tacctgtttt tggtttgtct tgatttgctt
     1381 cagattttcc tgaaagaaat tcttgagctg cttttaattc cgactctgat aaatctttct
     1441 ttggtaccca atgctcatgt gtcatatgtg gaatgacata acctgtacct gtatcttttt
     1501 taatatcttt agcatcaaaa atgtaaccat cagatgttgt gtatttacca gctttttttg
     1561 cctcttcaat ttcttgagca ctgtagacaa tctggctatt tggtttgcct ttacgttggt
     1621 ctttcggtgt tactgcatgg tgttcttcct ctgtgtgagg ttccttatta ggttcatgag
     1681 aaatatgctt tggtttctct gtagaatctt ttttccctaa tgcatcaata tgatgtcctc
     1741 tatgctctgc caccatcctt gttgcttcta actctaatgg agacatatcc ttatagtgaa
     1801 taaagtggaa atgattaccg tgcggaatag aaacaccgat tgctgtgaaa gcattaactt
     1861 gtgctgggtc ataaaccaaa ccatcacttt ctacgtgacg agcagataac ggttgagcat
     1921 ataaacgttt caacattttg tctaaagcag aatctttgtt gattggtttg gttgtaggtg
     1981 catggtgttt tggtgccgta gcatgattac cagctggttt tgaccagtga tttgcgtgag
     2041 tgactcttgg aatagaaata ggggcattag catgtcttcc tgttgataca ttaccgcttg
     2101 gtgcacttgc atgaatgtat gaacttgaat ttgatggttt tgagctatta cctgatcggc
     2161 cagtcttgcg gttccaatat gcctgtgcgg ctgataactc tgatggagat aaatcagctt
     2221 tcgggatata atggaaatga tcgccatgtg gtactaaaaa ggcatctccc atgtcatcaa
     2281 taacatctgt tggactaaaa acataaccat catctgtagt atagcgaccg ctagctttcg
     2341 cttcacgtac gctggctgat aaagcttgag tttttgcact actatgcttt cctgattctc
     2401 ccttagaacc acccttagac cattcttcgc gttgcttttg gatttgttcc tttgtacgaa
     2461 catttgttcg tttgctacct tctttaagat agaggtaata cttaccatta actttgataa
     2521 tataaccatc tttgacttca ttaatgacat cagctttatt aaagacatag ctagggtctt
     2581 tcataatcaa ttcttcacta atgatggcat catatggcac tttcccattg taataatggt
     2641 aatggtctcc atgagaagtt acataaccat catcagtaat tttaacaacg atttgttctg
     2701 cagatatgcc ttcttcttta ctgatttggt cgggcgtttt atttgcagct gttgcttgtg
     2761 ctttgctatc agatttatct gttttcacat aagaaacact attgtctgat ttttgggcat
     2821 tatagctacc taattggtaa gcacctacac cagtaccaaa tagagctact gataaagcac
     2881 taataataac tgttttttta cgattcatcc tgcgtccttt ctttatataa ctacctattg
     2941 atttaacgat ttgacaagta ccttaagatt agtttccaaa ttttctaagt aatctttatt
     3001 gtttttggga actgcttcta aaggacttaa acttgcaatt ttaactcgag tagctgaagc
     3061 tactgcttga gctaatttag gtgagactcc ttcttcaaca aaaatagtct taaccttata
     3121 tgttttcaca aactcctgaa tttcggctaa ttttttagca ctaggttctt gctcggttga
     3181 gacacctgca atacctaact gagtcaatcc gtatcgctta gctaagtatg agaatgctgt
     3241 atgtgaagtc acaaagtatt tagactttgc agctttaaat tttggcttat acttctctgc
     3301 aatagccatt gccttgtcac taaattgatc agcatttttt tgataaacct tagcgttttt
     3361 aggatccttt ttagctagtt gtgtagcgat gagttgtgct tcctcagata cttttacagg
     3421 gtcattccaa gtgtgagggt catacaaggt tgactcatct actccttttt ctgcctctac
     3481 atcttctaag ccatgaactt tatccaaagt catacctttt gaagcttcaa ttacagatac
     3541 tttagagtga tgcaaactag gttccaaacg tctcgcccaa gcttctagtg tgtgcgaatg
     3601 ataaagaaat aggtcagcat cataaatggc agcaacatca cttgatgagg gttcaaaacc
     3661 atgaatgcct gactgtgatc gaatcatttt aatatcattc aaatcaccag aaactgcttt
     3721 tgtaatggaa tatactggat aaaagctagt cacgaccgac aagccatttt tttgctggct
     3781 ctgtttagta caagcacata agcaaattag ggtaactatt ccgagaacaa aaataaggct
     3841 tttccgtatc cttttcatta aacctccttt aaaataaaaa gtacttaacc agttaacgtt
     3901 ttagagtatt cttattttag tgtcaacgaa aaaccataaa tctaatattt ttctattttt
     3961 aatattgtta agtgcactta atttcatatc aacaaccttt attcatgcct ttccaagcta
     4021 cagtgaatct ttttataaac atgaatgggt taaggagtat aattagaacc acaataaaaa
     4081 gagccaagca ttttgcccaa ctctttgatt ttttatttat ttttagtatc taaaacgata
     4141 gttactggtc catcattaat cagactgact tgcatatcag ctccaaattg acctgtttct
     4201 accgaaacat gatttgctaa ttcttgatta aagatatcat aaaactgatt cgctttaaca
     4261 ggatcagctg ccccagtaaa agctggacga tttccttttt tggtatctgc aaagagtgta
     4321 aattgtgaga tagataagac gcttccttta atgtcttgaa ttgaaaggtt cattttcccc
     4381 tcatcatcag aaaaaatacg catgctagta attttacgaa ctgcataagc aatatcttct
     4441 gttgtatctt ctggagcaat cccaactaga agtactaaac ccttctcaat agatccgact
     4501 acatcatctt caatagaaac acttgcttga ctaacacgtt gaataatgat tttcattttg
     4561 ctctcctaac cattcgtacg tttaacagaa taaacatctg ggataatttt gattttatca
     4621 accacagttg tcaattgtgc taaatttgaa attccaaagc taacatgaat attagcaaat
     4681 ttcatatctt ttgttggttg tgcatttact gtcgatacta gctttgttgc atttgataag
     4741 gtttgaagaa catcattaag gaggccacta cgattcaaac cataaatatc gatttcagcc
     4801 atgtactctt ttttagatcc atcatcatcc cactcaacat caatcaaacg ttgttcgtag
     4861 ttctcctgac tctttaaatt ttgacaatct gaacgatgga tagccacgcc tctaccttta
     4921 gttatatagc cttcaatgag atctccagga acgggattac aacattttgc aatacgcatt
     4981 aataaacctg aagctccttg aatgataaca ccattttcac tcttaacttt gagaacatca
     5041 cgcttatcgg ttttgatttc gccaccgtta ataagttcgt ctgcttccgc tttagcctta
     5101 gcacgttcct cctcacggcg ttctttttcc gtcagtttat taaaaatact aatcggactc
     5161 aaatcaccaa aacctacggc tgcatacaaa gcttcctcac ttttaacact gacacgtgga
     5221 aggatttctt caatatgttt cttatctaaa tatttgttag gcacataacc ttgttcttgg
     5281 aaataatcaa ccagtaattc tctaccttta ttaatagaag tttctttatc ttggtttttg
     5341 aagaactgac gaattttatt gcgagcttta ttggtcttaa caatcttaat ccagtcacga
     5401 cttggtccaa atgaattagg gtttgtgata atctcaacaa catctcctgt ttttaactta
     5461 gcagtcaatg gaaccatacg tccatttact ttagcgccag tagctttttc tccaacttgc
     5521 gtgtgaatag cataagcaaa atcaattggt ccagattccc ttggcaattc ctgaacagca
     5581 ccatttgggg taaaaacata gatgcgttct gtaaagatat cttccttaac agagtcaaca
     5641 aaatccttgg cgtcttttga ttcatcttga agctcaacta attcttgaat ccatcccata
     5701 ccaaccgatt gctctgcttg attaacctta ctggtgattc cttttttata ggcccaatgg
     5761 gctgcaacac caaactcggc aacttgatgc atttctttag ttcgaatctg aatttcaatt
     5821 ggccctttag gtccataaac cgtcgtgtga atagattgat aaccattggc ctttggtgcc
     5881 gcaatataat ctttaaacct tcctggcatc ggacgccata actcatggat ataccccacc
     5941 atggcataaa catcactagc agtttccata atacagcgta tcgcaattaa atcataaatc
     6001 tgatcaaagc gtttcttttt atcacgcatt tttctgtaaa tagaataaat gtgttttggt
     6061 ctaccataaa tatcaccata taaaccctgt tcctcagtat aggatctgat tttatcgaca
     6121 attatatcaa ccaactcttc gcgttcacga cgtttttcac tcatcatatg agaaatctta
     6181 taaaattccg tctcattcaa gtaacgaaaa gataaatctt ctaattccca cttgatacga
     6241 ctaatcccca aacgatgcgc taaaggagcg tatatttcca tagtctcacg tgaaatacgc
     6301 tcttgtttat cttttctgag gtgtttaagg gttctcatat tatgtaagcg atcagcgagc
     6361 ttaaccaaaa taacacggat atccttagac atagccatta acattttacg gtgattttct
     6421 gctaactgtt cttcatgaga tttgtactcc actttaccca attttgttac accgtcaata
     6481 atatcgcgaa catccttgcc aaaatcagtt tcaatctcat caagggtaat ttccgtatct
     6541 tctacaacat catgtaaaaa accacaagct accgtaacgg catcaaggtg taaatctgca
     6601 agaatacctg ctacttgaat tggatgaata atgtagggct cgcccgactt tcttgcttga
     6661 taataatgag ctgcggttgc gtaatttaag gcaaatttta caaaagcaac atccgtctca
     6721 ctcatgtact gggatgtgat tgcaacaacc tcttctcctg ttaaattaat ttctttgacc
     6781 atagttatcc tctttaatca tagtgataga ttgtacctat tttatcactt tttaacctaa
     6841 aaataaaaga agttagaggc tcaaaaagaa ttaaatacca agtaaatgtg tctaaccaag
     6901 ttatccttta taaataaacg attgttttta gtgtaattac taaaaataat tcgtaaatga
     6961 gattgattaa acgttttcaa taagctataa tagtttttag attaggataa tatacatgta
     7021 atgttttaat ggaggagatt tatgtcaaaa cattattttt caaaaagcat attcgcttta
     7081 actgtcttaa cagctacaac aacaagtggt ttagctgttc aagcagaaga cattgttaca
     7141 acaccaagtt caaccgctac aaaggttgaa tcaactaccc ccactagcac aattgcagag
     7201 gaaaagtcaa acgttacttc tactccaact gcaataactg atgcttcaac agccacaagc
     7261 acaaatacca ctgccaataa tcaaaatcca cagcctgttg ccacagaaac cacaactggt
     7321 gatctcaaac ctattgaagg tgaaaaagtt gaccttcgca tcatgtcaac cacagaccta
     7381 cacaccaatt tagttaacta tgattattat caagataaag aatctcaaaa aatagggtta
     7441 gcaaaaactg ctgtccttat cgaagaagca aaaaaagaaa atcctaatac cgtcttagtt
     7501 gataacggag atgtcattca agggacacct ttaggaactt ataaagcaat cgttaaacct
     7561 gtcgcagaaa atgaagaaca tcctatgtac caagccatga atgctcttgg ttatgatgca
     7621 tcaacactag gtaatcatga atttaattac ggtttagatt acctcaaaaa aatcattgct
     7681 acagccaacc ttccaatcct caacgcaaat gttttggatt tcaaaacaca tcaaccagtt
     7741 tttaaaactt atgacatcat tactaaaacc ttcaaagatt caacaggaag agctgttgca
     7801 ctaaatattg gaattacagg aattgtacct cctcaaatct taaactggga caaggctaac
     7861 ttagagggaa aggttattgt caaagattca gttaaagcaa tcgaagaaat cgttcctact
     7921 atgcgtgcta aaggagcaga tgtcattctc gttttatcac actctggcat tggagatgat
     7981 cgatatgaag aaggtgaaga aaacgttggc tatcaaattg ccagcatcaa gggagtggat
     8041 gccgttgtta cgggacactc acacgctgaa tttccatcag gtaacggtac tggcttctat
     8101 gaaaaataca ctggagttga tggtatcaat ggaaaaataa atggaacacc tgttacaatg
     8161 gcaggcaagt acggggatca ccttggtatt attgatttag gacttagtta tactaatgga
     8221 aaatggcaag tctccgaaag cagtgctaaa atccgtaaaa ttgatatgaa ctcaacaact
     8281 gctgacgagc gtatcattgc attggctaag gaagcacacg atggcactat caactatgtt
     8341 cgccaacaag taggtacaac aactgcgcca attacaagtt actttgcact agttaaagat
     8401 gacccttcag ttcaaattgt taacaacgct caacgttggt acgttgcaaa tgaattaaaa
     8461 ggaacacctg aggcaaattt accacttcta tctgctgcag caccatttaa agccggaaca
     8521 cgtggcgatg ctaccgcata tactgatatt ccagctggac ctgttgcaat taaaaacgta
     8581 gctgatcttt acctttatga taatgttact gcacttttaa aagtgaccgg agcagactta
     8641 cgtgaatggt tagaaatgtc cgctggtcaa tttaaccaaa ttgaccctaa caataaagca
     8701 ccgcaaaata ttatcaatac cgaatatcgt acctataatt ttgacgttat tgatggcttg
     8761 acttataaat ttgatattac acaacctaat aaatacaaca aagatggaaa agttgttaat
     8821 agtcaagcta gtcgagtacg cgatttaatg tacaatggta agcctgttgc agacaaacaa
     8881 gagtttatga ttgtgaccaa taactaccgt gctagtggta ccttccctgg ggctaaaaat
     8941 gccacaatga accgtttgct gaacttagaa aatcgccaaa ctattataaa ctatattatt
     9001 tcagagaaaa ccatcaatcc tacagctgat aataactggg gcttcacaga gtctattaaa
     9061 ggccttgatt tacgattcca aacagccgat aaagctaaaa atcttgttac taatagcaag
     9121 gatattcagt atatcgctag ttctaccaaa gatgaaggat ttggtgatta tcgatttgtc
     9181 tatactgaac aagaaaaagt agaccttact gagccatcac atcaagaaaa gttaagccaa
     9241 atagagaaac aaaaaattca ccacatggtt gcaactgctt atacaaaagc aaaagcttct
     9301 ccaaatatcg caggcactat ctatacacaa cagaaagaaa aaacattacc taaaactgga
     9361 caagaaaaac aatcttatct ggctttcttt ggcgtcgcaa tacttggact cgtgggaaat
     9421 ttacttaaaa agaaacaaat atagtcatca ctaaaaactg aggccaatca acctcagttt
     9481 ttagtctcaa caatagattt tctctctaaa cagtcagata ttgagatacc actctagctt
     9541 gtttatctga catgattttg ctaaactgat aacaagaaat ttagatttaa ggagtactaa
     9601 atgcctaaat taaccctagt ctatatcagt cttagcggca atactcaaag ctttgtcaaa
     9661 cgtcttagtg aacaactgtc aacttggcat aactgtcagg tttcgactat taatatcaag
     9721 gaacaaaatc atcaaacctt tcctgttgac caacctttcg tagcactatt acccacttac
     9781 ctcgagggag gaaatggcat tgacaatggc gatcaagaaa ttcttaccat tccacttggt
     9841 gattttatag ctgcacacaa taatgtcaaa aattgtatcg gaattgttgg ctctggaaat
     9901 agaaatttta acaatcagta ctgtttaact gcaaaacaat actctgaacg gtttggattt
     9961 cctatgttag gagactttga attgcgagga acttctagcg acgttgaacg tatcgcaaat
    10021 attatcgttg aaacattatc gaactttaaa gcctaaagga aatagtatgg aaacaacatt
    10081 aaactacatt aaaaccctga catcaatccc ttcaccaaca gggtttaccc aaacaatcat
    10141 gacctatatc atcaaagaat tggaagcgtt tggctactca ccaattcgca caaacaaggg
    10201 aggcgtcatg gtttctctaa aaggaaaaaa taatactaaa catcgcatga taactgctca
    10261 ccttgataca cttggtgcta tggttagggc catcaaacca gatggtcggt taaaaattga
    10321 ccttattggc ggatacacat acaatgccat tgaaggagaa aactgtacta tacacctctc
    10381 aaaaaatggt aaagaaattt ctggaactgc tcttattcat caaactagtg tccatgttta
    10441 caaagatgct ggaactgctg aacgtaatca aacaaatatg gaaattcgtt tagatgagaa
    10501 agtaacaact gctgacgaaa cacgtgcttt aggcatccag gtcggtgatt tcatttcatt
    10561 tgatccgcgt acaatcataa caggcagcgg ctttattaaa tcacgttacc tagatgacaa
    10621 ggtatccgct ggtatcctaa tggaacttct ttctgtttac aagaaagaag acattcaact
    10681 tccttatact actcatttct actttagtgc ctttgaagag ctaggacatg gagcaaattc
    10741 aagcatccca aatgaaactg tagaatatct agcagttgat atgggagcta tgggagacga
    10801 tcaagaaact gacgaatata ctgtctctat ctgtgttaaa gatgcttctg gtccttatca
    10861 ttatgaatta cgtcaacatc ttgtttctct agctgaaaac aataatattc cttataaact
    10921 tgatatttat ccttattatg gtagtgacgc ctccgctgcc atgcgtgctg gtgcggaagt
    10981 taaacacgcg ctacttggtg caggtattga atctagtcat tcttatgaac gtacccatat
    11041 cgattctatt caagcaactg aactcttagt ggatgcctat ctcaaaagca atatggtgaa
    11101 ctaatggtag ccacgctcat tattatcagg ggaaattcag ctagcggaaa gtctacaata
    11161 gctaaacaac ttcaagccga attaggggaa gatacccttc tcttatctca ggattacctt
    11221 cgtagagaga tgttaggaac taaggatggt gaaaatacta ctaccattcc ccttttgatc
    11281 aatttactaa attatggtta ccataactgt tcctacatca tccttgaagg catacttcgt
    11341 tctgattggt atacgcctgt ttggaaacat attttaaaac ataaccccaa caatacttat
    11401 gcttattact atgatttaag ctttcaagaa actgttaaaa gacattcaac tcgacttaaa
    11461 tctttagaat ttggagaaga ttctcttgca aggtggtggc ttgagaaaga cttcttaaaa
    11521 gagatacctg aaaaaatatt aacaaaatct atgagtttag aggatgtcaa aaaacttata
    11581 atgaccgacg ttttacctta acattattgt acgcactata aacaataatg acttcttatc
    11641 attggagtat attgcccatg gatacagtga ttgacctctt atttattatc gttcctatta
    11701 tagccggatt tctggcatgc attagtcttt atcgttattg gaaaaaagat ttcgaaagcg
    11761 acgaggacga tgattaaact cctcggaatc aatgattccg aggagttttt ctatattttc
    11821 atttaatcag catttctacg aatgcgatta taggttttaa ggatttggct cttatgctta
    11881 gtgacatagc gataatcttc tttaagagta gcgatatcct ttaaagcttt catgccatta
    11941 cttgtttggg catctttacg aataggtcgg ttactcgttg actgcccaaa ggcattttga
    12001 acatctaaag aaagcataaa attaataaat aactttgctt ctttcataga aggagcattc
    12061 tttataattg caaccgaaga tgggacaaaa actgtccctt ctgtcgggta tacaatagaa
    12121 acattggcac cacttttttg caaattgaca ctagggtctt cgtaagtcaa ccccacaatc
    12181 atttttcctt ctgcaactga ttgataaact tctgaagagc tagaagattt gatagcatta
    12241 atattatgtt gtagcttttt aacatagttc cacgcttttg gattggtgta accacccttg
    12301 gccaagagta tattagtgag ttgtgagaaa gcactagagg aagtattcgg atctgcaaag
    12361 gcaattttac cttttaagga aggctgtaat aaatcttcat aactcttgat ggtaagtccc
    12421 ttagctaatt cgttatttac aatcaagaca ctcccattta tagtataagg tgtcgccgta
    12481 tcactcggat ggatatagtc tggaataaca gtatgaacat tctttgatac gtaagactca
    12541 aacaatgcct tatgactttc aaattgcgta taatttcctc caaagaaaat atccgccttc
    12601 aactgcttac cctccttact taatctatct attagttgcc ctgtcccacc ttgaataagc
    12661 ttaactttta taccgtattt ttcctcaaaa gctggaatcg ttcctgttaa aatggcttga
    12721 ctatttggac ttagaataac taattctttt ggtggaagta gtttagaagg ctggctaata
    12781 ctgtatgtaa aaacacttat aaagataatg ggaacaatca gtagtatata aataagcctt
    12841 ttcgactgtt tttctttcat ctttcaccta cctaatccat ctgtttaaaa atatgttctt
    12901 ccttaaacgc ttttactgtc tgacccaagt actttttaaa aaccctatca aaataacgat
    12961 aatcataaat accaacagct tctgcaattt ggtaaaccat taggtctggt tctctaaaca
    13021 tcaactgaat ggcttgatta atacgatatt gatttatata atcactcaaa gttatatgaa
    13081 ggtgcttttt agtgactgtg taaagataac tttcactgta acctaaatcg tgagctaact
    13141 ggctaatcac aattttccca tgaaaatggc tatgaatcca tgctagcata tctttaataa
    13201 cttcaggaac ttggtcatta gcttgtggta gaggtaaata caattctgta tgctcttgta
    13261 ggtgcttctc cttatgttcc tcaattctag ccataattgt ttttaaacaa tcaatcaaag
    13321 cttgcttttc tagtggtttt gttaaaaatt caagaacgcc atagtgaata gctgattgcg
    13381 catttggaaa atcagcatag ccagataaaa taattttggc ataagattgt cctttggttg
    13441 cctcaaacat atcgaatgct gtcataattg gcatattaat atcacttaaa acaatgtctg
    13501 gctgctcttc ctgaattaat tgagctcctt ctttaccatc ttttgcttca ccaaccacta
    13561 aaatattcaa tgattgataa tcaatagcat agcgcaacca cttacgaata aggtgctcat
    13621 cctcaacgat aagtaatcta tacataactc actcctttac tatatagcta actcgaaaag
    13681 catcatctgt cttcgtaaac tctaacaata ctgttgaaaa ttgatattgt aatcgtctat
    13741 aagaattaat aagcccatga tgcgagtgat ttgatcttaa cattgtacgt atagcttttt
    13801 gcctagccaa actaataccc gatccattat ttgaaacagt aaaccaaatt ccatcactat
    13861 cttgccaaat atctatgttt attgctacat catgacgttc ttttaagcca tatttaatag
    13921 cattctcaat tagcggtaac aagaaaagtt ttggtacacg catgtgttca agttcaggag
    13981 atactgaaat agtgtaagtt aattcttcaa atctcacttg attaatcaaa aggtaggatt
    14041 caatgattgc taaatcatcc ttaagcacag ctgcctctgt gctaccacta aggctatagc
    14101 gcaataattt tgttagttgt ataacgattc tttctgttaa ttgtgaatca tagtgactgg
    14161 ttattaaaat tgtctcaagg gtattataaa ggaagtgagg attaaactga gcttctaaca
    14221 tcctcttttc aaacagcaac ttttgtgttt ccaaatcgag agttttctca tgtaaatctt
    14281 ttaaccgtga aaccatctga ttgatttgaa cagatagata ttgaaattca tcttgactat
    14341 caagctcaat actagatttt tcttggcggg aaatggcatc catatctagc accatttgat
    14401 ttatggcgcc agaatttttt actgcgatgc gatcggctag aactctagat tttcgtcgta
    14461 aaatcacaaa aataataact gatgatacta gagaaaaaag gacgaccaga gttactggta
    14521 tcaggggacg atacatataa agtaggatat tatcttgcag agctactttc ctaacaacaa
    14581 aagctcgatg atcgtgaaaa ctaaaatatc tagttaagaa ttgactatct accttatcta
    14641 aactagagga gataaaatca cgattagtaa aagtaaagct attatttagc tgatcggcaa
    14701 taatcaagtc tgagttaata gccttcgttg gaagtaaaaa gtctttccca ttcatcaata
    14761 aaaaggcata acctgaaact tttttattaa ctataattgg cttaatgagt gcaaggtaat
    14821 gccctccctc actatctaca ataattttta gtagcctttg atttatttta gccttatcta
    14881 aaacttcact aatgtaaata gacttactaa aaaaattacc taaatgtgaa ttactagcaa
    14941 acaaaagttc ttgattaggt gagaatacta aaagttctgt ttgactttgt aaatgattcc
    15001 ttaaatgata atattgctta ctcaaatcag cacttcctaa gtgtcctgag acaaatggtt
    15061 tcaaactatt tttacctaag tcatctaata aggtatcatg tttttgaatc aattgcttat
    15121 aactatagga aactgaatta acatctttat aaaggcctat ctgctgaggc gctaaagaaa
    15181 aaaggacgaa aagtgcaatc aataacaagg ataaaattaa agactggcgg gaaaaatgtt
    15241 tagagatatc atcttgtagt cgtttcttaa agcgttcttc cattctatat cctctcattt
    15301 ccatctcctt ttattatagc aaatagctat ccaataaaga tagtcaactc ctttcataca
    15361 ataactttca aaagaaaaaa agataagcta aacaagtatc gtcttgttcc acttaccttt
    15421 aatgctagga gattattagc aaatacctaa gagaccaagt acaataccta acaccataat
    15481 accaaacatg atccagttga tattgacttt tttacgtaga agccagaagg ctaatatcgt
    15541 taccaacagt ggcactacgc caacaaataa ttggtcaagg taactttgta tggaagctgc
    15601 ttccttagct cctttagctg ctaccgttaa gattgtcttg aatttaacat ttgaagcagt
    15661 cattgagcct accatcatta aaccaagcat acttgaagtc ttagtaacaa ttttaatacc
    15721 acctgattca tacaattttt tgataaaccc tgcacctaca gagtaacctc catagagact
    15781 ataataatga atcagaaaag ctggaatgtt atacaagagt agaaagacaa cagctcccat
    15841 ggctgagcct gtacttgcta aggagatacc gatacctgcc gcaataaccc ttagaattcc
    15901 ccaaaagaat gaatcaccaa caccagatat aggtcccatc aaagaagcct taactgcggt
    15961 gatagcacta gcgtcaaaat caggatcttc actgtttttc ttttccatag atgcaacaag
    16021 acccataata aaattattaa tgtgcatggt ggcattaaac catgttgtat gacggaccag
    16081 tgcctctgct ttatccttat cagtcttata aaaacgattg atgactggta gtaaagtata
    16141 aataactcct actgcgtggt attgtgttgc cccagtacga ctggcattca tcgtccatga
    16201 gcgccagaag actgagcgaa gcattttacg gtcttcttta gtcaaattat cctgtgattt
    16261 catgcgaaga agtcctcctc ttctgattct ttagagtcaa aggttgtctg tttactgatc
    16321 gcacctctag taattgcgtc taattctata tcacgttgtg aactaatgac gcaaataact
    16381 gctcctaggg cagcaactgc aaccgctggt aattttaaat aagctgttaa aacaaaacca
    16441 agcaagtaaa aaacagctaa tttatttgtc cataataatt tcatcagcat ggcaaaacca
    16501 acagctggaa ggaggcctcc cgccgcactc aagccattca tcaggttttg aggaatatga
    16561 tggacaaaac tattaacagg cccactacct actaaaatac caatgaaaga aatagaagcg
    16621 ataatgagat agtaaataat ccatgtacca tagtgaagca tcacaagttt accttgatgg
    16681 tttgcggctg cggctttatc caccatcggt gcaaagatat tcatgaagac gtttttcaaa
    16741 aacataacaa caaatgctgc caacattcca atcggaacag ctagagtcat tgctgctttt
    16801 tgatcaatgt ttgaaataat ggtaaaagtt gttgccatcg ctgttgctgt tactggctct
    16861 gcggcaataa cacccccaat attaacgttt cccaaaaata gcgcttctag ggaagcaccc
    16921 attaagatac caaccttaat atcacctagt agcattcctg ttacaagacc aactacaagt
    16981 ggtctctcca tcatactttg acctgttaag taatttcccc caaaacaaat caacacagct
    17041 aaagctgcca tcgtcgctgg cattatcata tgtcatacct cactttgttt gactagctga
    17101 ctcaagttaa ccttagtatt tgaaggcaat acttggttaa agacgtctaa atctggtaag
    17161 ctggctaatt cttttgcggc ttccaactct tctggattta ataagagact tgatgttagt
    17221 ttgacttttg tagctggatc agacttatca aagcgtccta cattagcgac gtttactgct
    17281 tctaaatcgg ttatattttt agcaattgta caggcatctt taacagaatt gacaatcaca
    17341 aaaatgcgct tgtcagttgc gcgtggatct ttaaatagag ctattgattc ttcaactgaa
    17401 cggattaata atttcatgcc attaggcaca gccattttaa gagtcatctg tgtgatttca
    17461 tttttggcag cctcatcatt agccaccact aaaagtggtg catttaattc tttagtccat
    17521 acaactgcca cttgcccgtg aatcaaacgg tcgtcaacac gaatttgagt aatcattttt
    17581 ttcctcctaa ttattaatcg aaaaagtcat cttctgaaca cgctttaaag ctgagatcta
    17641 ccagctgcac ttgagattca ttgataacgg ttacaatttc ttctttagtt aattcctctt
    17701 caggtttcaa aaatagtaat gacaacaagg tcgctaaatt gctgttcgtt attaaaaaaa
    17761 cattattttt cccactattc attacagcag tcgccatttt ctgattaacg ctgccaccta
    17821 acaaatctgt aaaaataagc ccctgttcat ctggtgcaat ctcatcaata aattctacaa
    17881 tagacttggt atagtcacta tcatcaatgt aagcatctat tgtctgaatc tcttgtccct
    17941 tgcctgtcag aatatcaata gaactctgca acccactcgc aagttttccg tgactgccga
    18001 ttaggaattt ccgcttcatc tttctcctcc tatttattac attaccctaa attagttctg
    18061 aaagttttat caatactttg aataagataa cctttactat cattctaaca aatgaaaacg
    18121 gtttaaaaat ggactattct actctatgtg tggactattc tatataatta atatttaaaa
    18181 acagaacaat aaaaaggtca tgtatcctaa tagtttacac aacctttaca tcactataac
    18241 tttaaacttt ctcttatatg aatcactatt tcgaaagaga taatggaata attttttgat
    18301 agggtatctt attaacaacc tttattatga gtagagcaca agcatgtgtt aatgccaagg
    18361 tcaccacaaa aatcactttt ccttgatggt gtgcaactaa ggaagttgat tttaaacagt
    18421 aagacactat gccaacaaaa agagggtgaa ggaagaaata atagacactc aactgtttta
    18481 aaggacttaa acgatagccc ttaaacagtg atgtacgtat actccagtta aataagaata
    18541 ctgcacataa aggagctaat aagaagaaat ttttatccaa cccttgatta aaataaataa
    18601 gaacattctc taaagctaga agtaaaagga agagaatata cttaagacca cgcgatttgg
    18661 taaataggtc agtattaaaa taatcataga gtaaatatcc tgctagaaga taaactggag
    18721 tgtaaaataa tccgtttcgt gttgtctgaa aaattgacat ataagtggac atcaggggat
    18781 aaaattttgt tgactgtaca aagacagaat aggtctctac tgctccaata caatagagta
    18841 caacaactgt gataaatgtc cccctagttc ctacatactt caataatcct tgaataatga
    18901 accaacctag aataacagca gggatgtacc aaagagtatg tgacatacct aaatacaaaa
    18961 atgccacaat aaaacccggc agcaaataaa tcttcgctat atttagagat tcaaaaaaga
    19021 aataaccata aggcaaataa atgatagtcc acatgctata ctttttaatc agtttacgaa
    19081 agtaggagtg attacacaaa ccttgctgta tacgacctct caaaaagaaa gcagtacaaa
    19141 tcaaaaaata aggaacagcc atccgaccta ggaaactttt aaaggtaaaa tgaatgacat
    19201 cctgactaaa taaccttcca ctatgaacta aaatgaccag tatggaaaaa agatattgta
    19261 gcaagctaat cattgatgta ttatgctgct tattatccat gcactgactt ctcttcatcc
    19321 ttaacattac tttctataag ttatcattac tatgatacca atctcttcag aagaatatct
    19381 caattatccc caaaagtcat tgaaacgcct taaatggtat ctgataatta ttgttaatac
    19441 cttatcaacc atattaggaa ataaaaaagc tgatttcgca aaagaaaatc agcttagaat
    19501 aatttcttgt tcagataacc agtgggaact ataaacttct tcgtcgcatt aaacgatagg
    19561 tataagctat caataacact gcaattaaca tacgtagact aatcagacag atttcattag
    19621 cagaaaaact atcaaagaaa aagccatgac taccattacg tgataatttt aaaagtaaag
    19681 ctgaagtgtc tgtcgttgat actgagaaaa tagttaataa cttgtttatc acacctttat
    19741 taaacatagc cattaaaaag ctaggagcaa aaaaggcaag cgttgaaaca gcaaaagatg
    19801 ttaatggcga atttgtgata cttaatataa agaaattaag actggctata aaaaggagtc
    19861 caacaaattg taagacaata atatgaacaa aagcagttga tattgtcata ggtacaggaa
    19921 atcccataat attagccaaa ggtgatgtcc agtataaatt cagttgaaca cttgtctccc
    19981 aaccactaaa accaaaataa tctgcaaata ccgtcagaat gagggcatat gctaggataa
    20041 atgtggaaat accgatggtc attgtactag ctaacttagc ccaggtcaac tgaccacgtc
    20101 cataccgagt cgtcagtagt ataggattaa tcttcttagc cctatctcct gaaaaaagag
    20161 gagctgataa gaatagagta aataagacaa ttaaaatata agtactatta cttacttcaa
    20221 aaagttgatt ccaaggagca aagttaccta gctttagttg ttttagtggt agcgcagaac
    20281 ccaactgttt ttgatcttta atatttattt tattaaaatc aattgtttta tcatctttca
    20341 attggttata gtaaattaaa a
//
LOCUS       DATRNX010000031        20319 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRNX010000031 DATRNX010000000
VERSION     DATRNX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(65..1369)
                     /locus_tag="VBC81_000893"
     CDS             complement(65..1369)
                     /locus_tag="VBC81_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6911946.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(1516..2415)
                     /locus_tag="VBC81_000894"
     CDS             complement(1516..2415)
                     /locus_tag="VBC81_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6911947.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(2608..4155)
                     /gene="purH"
                     /locus_tag="VBC81_000895"
     CDS             complement(2608..4155)
                     /gene="purH"
                     /locus_tag="VBC81_000895"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6911948.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(4175..4927)
                     /locus_tag="VBC81_000896"
     CDS             complement(4175..4927)
                     /locus_tag="VBC81_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6911949.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(4950..5498)
                     /gene="purN"
                     /locus_tag="VBC81_000897"
     CDS             complement(4950..5498)
                     /gene="purN"
                     /locus_tag="VBC81_000897"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6911950.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(5665..6687)
                     /locus_tag="VBC81_000898"
     CDS             complement(5665..6687)
                     /locus_tag="VBC81_000898"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6911951.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(6715..8169)
                     /locus_tag="VBC81_000899"
     CDS             complement(6715..8169)
                     /locus_tag="VBC81_000899"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6911952.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(8403..12128)
                     /locus_tag="VBC81_000900"
     CDS             complement(8403..12128)
                     /locus_tag="VBC81_000900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6911953.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(12251..12955)
                     /locus_tag="VBC81_000901"
     CDS             complement(12251..12955)
                     /locus_tag="VBC81_000901"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6911954.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(13079..13318)
                     /locus_tag="VBC81_000902"
     CDS             complement(13079..13318)
                     /locus_tag="VBC81_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6911955.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(13329..14321)
                     /gene="plsX"
                     /locus_tag="VBC81_000903"
     CDS             complement(13329..14321)
                     /gene="plsX"
                     /locus_tag="VBC81_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6911956.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(14399..15277)
                     /locus_tag="VBC81_000904"
     CDS             complement(14399..15277)
                     /locus_tag="VBC81_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6911957.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFIPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLLMFFIYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMT
                     TVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVT
                     GVHTAWNFTMGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVG
                     CLAYFSLERVKVSVRY"
     gene            complement(15340..16101)
                     /gene="recO"
                     /locus_tag="VBC81_000905"
     CDS             complement(15340..16101)
                     /gene="recO"
                     /locus_tag="VBC81_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6911958.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFYDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(16091..17266)
                     /locus_tag="VBC81_000906"
     CDS             complement(16091..17266)
                     /locus_tag="VBC81_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6911959.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEASTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(17374..18342)
                     /locus_tag="VBC81_000907"
     CDS             complement(17374..18342)
                     /locus_tag="VBC81_000907"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6911960.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            complement(18466..19791)
                     /locus_tag="VBC81_000908"
     CDS             complement(18466..19791)
                     /locus_tag="VBC81_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6911961.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            complement(20027..20114)
                     /locus_tag="VBC81_000909"
     tRNA            complement(20027..20114)
                     /locus_tag="VBC81_000909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20078..20080),aa:Ser,seq:gct)
     gene            complement(20128..20201)
                     /locus_tag="VBC81_000910"
     tRNA            complement(20128..20201)
                     /locus_tag="VBC81_000910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20165..20167),aa:Ile,seq:gat)
     gene            complement(20236..20306)
                     /locus_tag="VBC81_000911"
     tRNA            complement(20236..20306)
                     /locus_tag="VBC81_000911"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20272..20274),aa:Gly,seq:tcc)
BASE COUNT         6199 a         4435 c         3550 g         6135 t
ORIGIN      
        1 ataagaacct tgaaagtcta ttcaaggcat ataaaaaaga agccaaatag cttccttttt
       61 tatattattt gttaaatgat acgtgaacgt ggtcatagtg gttggcagta acgccaccac
      121 gatctggcat tgcattccaa gtattagcag gtccataaat actatttgta tttgaataaa
      181 acttttgttg ccagataaca tatgaaatgt tatttgctgc catattttgt gtagagtact
      241 gtgcaacttc attaccaagt gcttggtttt tacctacaat aaagtcaact gctaaacctt
      301 taccatgatc acctggatct cccgcacggt atgtactgaa ttcattaact ccataagttg
      361 acgctacttt ttctttataa gctgcaacat gaggttggag ccctgcattt tcaggatgtg
      421 cagctactgc atttgttgtt gaagctggtt gtgctaccgg tgttgctgtt ggagcttttt
      481 gtgctaccgg aacgctctta acttcagtcg cttgtaactt actgtctgta gctggtgaag
      541 tcgtagtcac aggaactgct ggagctgata catgctctgg tgatgcacca gtttctactt
      601 taggagtgac tactttagca cttgccactc taggggctgc tacagttctt accggtgcta
      661 ctttagctac tggagctggt gtttcagcgg caacagaagc tggtgatact gttgttaact
      721 gactgactga cgtctgagtt ggtttaactt cctctttagc tgctggaact tctgaagtaa
      781 tcgacttcac aggagctggt gatacctgtt cattagctgc tacttgacta acagcttgct
      841 cttgtgctaa tacttctttt gatttcaaag ctggcgcaga agaatatgtc ttcattggcg
      901 aaacaatcgt tgttgctgct tctggtgtca taccttccga aattgtattg agagaaactt
      961 tttggtctgc aacaaaaact tgattggttt tcaaatcgac agtagctgtt gtttgaccag
     1021 cagcatttgt tgctggtgtt tctattttca ttgaagtggc agtatgactc ttctgatcgt
     1081 aagttactgt cagtgttgtc tcaggataaa taagattgat atctgcaatg ttattaattt
     1141 ttgctaagac attcatatca attgacattg cttctgaaat aacgcttagt gtatcaccat
     1201 atttcacagt atatgatgat ttattgtctt gctttaccaa atcagccttt acctctgaaa
     1261 cagtacgtgc tgtccacgtc gtatctgttt cttgtgcttg aacacttgcg actgataata
     1321 gcgaagctgc cattgtcgat gtcaatagta cctttttatt cattttcatt gcttcaattc
     1381 ctatttcctt tttattagtc cgagactata atatcacact ttactggact ataaaattat
     1441 tttcagacgg tttaaagaaa ttgtacttag ttagtaacaa aactgtcatt taaaacaatg
     1501 aatcaatacc tcaaactatt tgtaaagcag ttcttgactg ttgtcaacgc gaagccaaac
     1561 tgaactacca ttagcaaagc gtgtcttagc ccaagttaaa ccagctgttc cttggatggg
     1621 tttttctaca gtcttaagag ttttaggatt aaagataaag taaccacctt tttgaagaac
     1681 ctggtcagct gtcaaattac cattagcatc aacctcatca atttctgatg ctggtatacc
     1741 attatcgttc caatcaaacc cagtcggcgt tagggtgtta tttttgacta accaaacacc
     1801 attaaccttt tgtaattcat ctactcgata cacttttaat ttactagatt gattctgtac
     1861 aggagctgat tgtaatggct taatgcttgg agctgatgct tgcgtttttc cactaaaggt
     1921 cgcaacgtta gcaattagtg acgttggatt gatacgtcca tggaaaccat tttgaaaatt
     1981 agggttagct ggtaaaaatt caaaatgaag gtgaggtccc gtcgccatac cagttgctcc
     2041 tacgtaaccg atgatatctc cttgtttgac tttttcccca gtcctagcca ccacacgtga
     2101 catatgagcg taaccactat gcattccatc cgcatgttga atcatgacac aatttcctgc
     2161 taagtctgtc atccaagaaa agttggctcc agctcctgca aatttcacag taccatctgc
     2221 cactgcccta ataatcgttc cagtcggaac agcataatcc accccacaat gtccaggata
     2281 accattgaaa cctgttgtaa ttctaccatt atcaattgga cggacataag tatccgctaa
     2341 aactcgggaa cccgcagata aaaccatacc acctaaaaca actaaggaac ttgccttaac
     2401 taaccattta ttcaagaaaa acctccaaat agaattaata taatgaagtg ctcaaacact
     2461 tgcaattaac ttattcgaaa atttaaatca aaatatcaaa aaaagtactc accttgatga
     2521 gtacttagga ggtttaataa aaagaaaagt ttttaggata tacgtagtat acctgagtta
     2581 acttaagaaa acctaaacta ctatttttta atgtctaaaa tgtctcacgc ctgtgaagat
     2641 catggtcaag ccatgtttgt ttgcggcgtc aatagattct tgatcacgaa ctgaaccacc
     2701 tggctggatg attgctttga tgcctgcggc agctatttct tcaatgttgt ccgcaaatgg
     2761 gaagaaggca tctgatgcta gaacggcacc gtcaaggtgg tccttagcct gctcgatagc
     2821 aatcttgact gagccgacac ggttggtttg acctacaccc agtccaagcg tcatgtgatc
     2881 gttagtaata ataatcccgt tagacttaac atacttgata gccttccagg caaactcaag
     2941 ggcagtcacc tcttgttctg ttggctggcg gtctgtcacc acttgccagt cagatggatt
     3001 ttcagccaca acgtcttggt tttgcaccaa aagtccacca actacgccag tgtactcagc
     3061 ttccacttcg ctggcagctt gggcatcaaa cggcaactca aggatacgca agtttttctt
     3121 tttatttgtg agaatagcta gcgcttcttc tgagtatgat ggtgcgatga tgatttccaa
     3181 gaagataggg tgcatcttct cagctgtcgc tgcgtcaact tcacggttaa ggacaacaat
     3241 tccaccaaag attgaaactg gatcagcttc ataagcgtaa tcccaagctg tctcaatatc
     3301 atcagcctgt ccgataccac atgggttcat gtgtttgagg gcaacaaccg ttggactgtc
     3361 tttgaaatcg cggataatac ggattgctgc atcagcatca cggatattat tgaaggacaa
     3421 ttcttttcca ttgagctgtt tagctgaagc gattgagtag tctgttggta aggctttttg
     3481 gtagaaatca gcgtcttgct gtggattttc tccgtaacgc atagcctgtt taagatcata
     3541 agtgatggtc aatttttcag gcttagcctc tcccacttga gctgtgaagt actcagcaat
     3601 caaagcgtcg taggctgccg tatgacggaa ggccttagct gccaagcgtt gacgagtttt
     3661 aaatgtcgtc tgactagcgt cagccaattc tcccaaaaca gtggcatagt cagctggatc
     3721 aaccacaacg gttacgctag cgtggttttt agcagctgag cgaagcattg atggaccgcc
     3781 gatgtcgatg ttttccaccg ccaaatcgtt ggtcacgtct gggcgaagga tagtctcctt
     3841 gaagggatag aggttgacaa ccacgaggtc aatcaactcg atgttgttgt ccttagcagc
     3901 ctgaaggtgg ctgtcagcgt cgcgacgagc caaaatccca ccgtgaatgt ttgagtggag
     3961 ggtcttaaca cgaccatcca tcatttctgg ggagccagtt acatcgtcaa tagcaatggt
     4021 ctcaacacca gcatcgtcaa gggcaacctt agtcccacca gttgagataa tatcccaacc
     4081 caagtttttc aattcttttg caaagtcaat aattcctgat ttgtcagaaa ctgagattaa
     4141 agcacgttta gtcatgagtt ctccttttct tacttcaatc tatttcatag gctatgtatt
     4201 catgagaaaa ttcataaccg agtttttcag ataaattcaa tgaagtcctt gtatgagcat
     4261 cccagctagg ataaattccc ttatctaaac aagttagtat caactgagct gcaactattg
     4321 ttgctaaacc acgccgacga aaatctggat gcgtatctac ttctatctcg atcccatttt
     4381 tataggttga ataagatgaa gctcctgcaa tgatattccc ctgataatat ataacataac
     4441 ctataccttg ttttttatag tactgataag tagcgtaatt tgctactaaa tcctgtgacc
     4501 attccttttc taagcaagag ttgtaaactt tctcatcaat aacacgtaat tcaaaaccat
     4561 ttggcaactg agtaacaaac ttctctaacc tacttcgctc aaacaaagta tcttttttcg
     4621 tcgcataacg cttaaaagaa tgggcattct ggccataagt tgattcaatc aaatctgacc
     4681 atcctttatg ctgaggaaca aggataatat cctccccaga gcaaacttct agtagaaata
     4741 aagtaggctg acctgctaga aagccaaaag atgatttcct tcccaatttt gccaaagaag
     4801 attttggttg gtctaaacta tctacaaaaa cttctcccat aataccttgg acacacgacc
     4861 agattatggt ctcgtcccaa tcaccaaaaa tagactctac tatctctaaa tttttcacta
     4921 gtttcattag cttattataa caaattttac tattttctct ttatgcccaa actatccaaa
     4981 acatctggat agagttgata ttcggtttca tggatgcggg tttcaaagct ttctaggcta
     5041 tcgtctgcta ggcgtggcac gcgcacttgt tggatgacct gaccggtatc cacaccagag
     5101 tccacccaat ggatggtcac accagactgg tcaacacctg cctcccaagc atccttgata
     5161 ccgtgagcac ctggaaattc aggcagatag gctgggtgaa tattgatgat acgcccttca
     5221 taggctgaga gcaaggtttc tccgacaatc ttcatgtagc ccgccagaca gaccaagtca
     5281 atctcgtgtt tgtccaacaa atcaacgacg gcttgctcgt aagccgcctt attctcaaac
     5341 tctttgagtt caaaagcgaa gcttggaatg gctaagttct gagcacgctc taaaacatag
     5401 gcatcacgat gatctgaaaa gacaaaacta actggaaact gctctgctat gacctgaaag
     5461 ttggaaccat tagcagaagc aaaaacagcg attttcatat tcgatacaaa gggatgattc
     5521 ggcttgccga aaaatttcta gaaaaatagg aaaacgaaca acgacgcttg cgtctaagtc
     5581 gttttatctt ttttcccaga aatttaatcc cgtgttcagt taggcaaact aactaaacac
     5641 tcctttctta taaaatttag tgacttattt aatcaccaca ctatcgtctg ctttcttgat
     5701 gatacgaccg atttcataaa ctggttcgtc caagagctct ttgacgcggt taacattttc
     5761 aggactaaca gctagcataa gaccgacacc catattgaag atttcaaaca tttcttcgtg
     5821 cttgatgtca ccatattttt caagcgcctt gaaaatcgga agtactggca ccttatcctc
     5881 atcgatttcc gcagccaaat catccgcaaa catacgagga acattctcga taaagccacc
     5941 acccgtgatg tggccgatac cgttgactag ttcttccttg attaatggca gagctgcttg
     6001 aacatagata cgagttggct caagaaggac atccttgagt tgtttacctt caagctctgg
     6061 aagcagctct ttaccagtat agtcagcaaa gacacgacgc accagcgaat aaccgtttga
     6121 gtggatacca cttgaagcaa gtcccaaaag aatatcgcct tcctttactt tggaaccatc
     6181 aatgagctga gatttttcag ccacaccaac agcaaagcca gcaaggtcat aatcatcttc
     6241 gccatacatc ccaggcattt cagcggtctc acctccaatg agtgctgctc ctgcttgaac
     6301 acaaccttct gcaacgccag aaacaacttc ttcaagtttg actgggttat tttttcctgt
     6361 cgcaatataa tctaaaaaat ataatggctc agcacctgct gcaataatat cattgacaca
     6421 catggcaaca caatcttggc ctatggtgtc atgctgttga tatttaatcg caagcatgag
     6481 ttttgtgcca acaccgtctg ttcctgaaac caaaataggc tctctaactc ctgttttact
     6541 caagtcaaac accccaccaa agccacctag agctcccatg actcccgcac gttctgtacg
     6601 agaaacgtgt ttttttattc tttcaaccac ttcataacca gcttcaacat caacaccaga
     6661 ttttgcataa gcattttttt cagacatgtt ttctccttat ataattagca gagcttattt
     6721 cactttttga atataaaaac ttgtttcttc ttctaaactt ctgaggtatt cttcttcata
     6781 atcataaagc ggtgttggat aatgtccatc aaagtaagct acgcataaac caccatttgg
     6841 tgcttttgtt tcgagtccaa ttgattcaat caacccatct agtgagagat aggtcagact
     6901 atctgcgcca ataatatcac acacttcgtc agcagcgtga tttgctgaaa ttaattctcg
     6961 tcttgtttga atatcaatac cataaaaaca aggatacttt aattctggac tagctatagc
     7021 aacatgtact tcactagctc ctgcttctct taataatccg acaatccttc tagaagtcgt
     7081 tcctcttaca attgagtcat caatcataac aacgcgcttt cctttgacaa caccagatac
     7141 cgctgatagt ttcattcgaa caccttgttc ccttaattct tgtgtcggtt gaataaaggt
     7201 tcgctgcgta tactgatttt ttacaagacc catctcattt ggtaatccgg attcttcagc
     7261 aaagcccata gcagccgata aggacgaatt tgggacacca attacaatat cagcatcctg
     7321 tttaaattct tgtgcaagac gctttcccat attttttcga gccgtatgaa cattaacacc
     7381 atgtatagtt gaatccggcc ttgcaaaata gacatattcc atcgaacaaa ttgccagttg
     7441 cgtttcatca gtataacgat cacattgaat accactatcg tctataagga taacttcgcc
     7501 aggttcaaca tctctaaccc attttgcgcc taccacctca aaagcacagg tctcactgga
     7561 aataacccaa gcaccatttt gcatttgtcc aattgacaaa ggacgaaagg cattagggtc
     7621 aagagcagca attaatttat cttctgtcat cagtagatag gcgaagcctc cctttacagt
     7681 gcttaaagct tcttttacct tccccataaa acttgggtta tggcttcgac gaatcaagtg
     7741 catcaaaatt tcagtatctg aggaggcatt gaaaattgca ccttgctttt ctaattcttt
     7801 ccttaaagaa atagcatttg tcaaattacc attatggcat aaagcaaatt gcccgtcatg
     7861 aaatttataa agaaaaggct gaatattgcg aatatctgca gaacctgcag tagcataccg
     7921 aacatgtcca atagccgcat tcccagttaa attatctaat tcagattgat tcttaaaaac
     7981 ttcagaaagg agcccaacat ttcgataacc atagagtttc ccattatcat tcgaaacaat
     8041 accagcacct tcttgaccgc gatgttgaag gctatgaagc ccaaagtaag tgacttgagc
     8101 tgcctgagga tgcccccaga taccaaagac tccacattct tcatttagag attttacttc
     8161 gtatgtcatt cttttttcct aattcttttt gtatctcatc ttaagtagct ttgatgattt
     8221 tctcaatttt ggatctatcg tcaccaatgc caacatcaaa atagaaaatc tatcaagcta
     8281 ttttcaagct aatcattgag tcttagcctc aacggctccc aaataccact acgacagaaa
     8341 gatacagtta agctcgcttc gctcttgttt ccttatctgt tttatgaaaa aatttgaaaa
     8401 aattattttc ctgtaaaata ttttactgca ctagcaaaca aggcttggtc tttgttacca
     8461 gggatatttt ggaagaggcc gtcttcccag cgttctgagt gccccatctt accgatgatt
     8521 tgaccattct tgctggtaat cccttcgatg gcattgacag agccatttgg attgtattta
     8581 gaatccatag atggttgtcc gtcaaagtcc acatattggc tccagatttg accattgtct
     8641 cttagctctg caaattcaga agcgctgaca acaaatttac cttcaccgtg tgaaactggg
     8701 atggcatgaa tatcgccgac ctcaactcct accaaccaag gtgagttggt atttgcgata
     8761 cgagtctcaa ccatcttggc aacgtgctgg ttggcatcgt tatagaagag ggttggactt
     8821 gtctcaccag cttcctcgaa gtttccgtat ggaagaagac ctgatttaac aagggcttgg
     8881 aaaccattac agataccgat gataaggcca cctttttcga tgaagctgtc aatagctgcg
     8941 cgaaccttct tgttaagcaa gatattgacg ataaacttag cagacccatc tggttcatcc
     9001 gctgctgaga atcctccagc aaagaagatg atatttgcct tagcaatatt agcgaccatt
     9061 gtgtcaactg agtcagcaat agcagcctca ttcaaggtta caaatggtac caagttgaca
     9121 ctagctccaa cctgttcaaa ggccttagct gaatcatatt ctgagttggt accagggaag
     9181 actggaatgt aaaccactgg tttttcaatt gtttccttag ccttgataac agtatctgat
     9241 accacagcag gaacttcttc aagagcgtct gcctgttcaa actctgttgg gtaaacctct
     9301 tccaatttgc cttcgaagac tgatagaagg cttgccccag caaggtcatt tccattgaca
     9361 gtaagtgtaa aggctgcctg agtttgaccg attttcaccg agtcagcaat ttcctcagct
     9421 gatgtaaaga caaagcctcc gagttgagct gtcaagctac tgtcaagctc tgcaatttca
     9481 acagaggccc cgatacggtt accaaaagtc atgagagcaa gactttctag gacaccaccg
     9541 tatttaacag ctgaagcagc agtaatctta tgttgagctt gaatagtctc gaactggcta
     9601 aagttagcct tgataaggtc aaaatcaata tcttctgaaa tagcttgacc tggaatgtag
     9661 tagatattct caccagccgc tttaaactca ggagagagaa ccttgcggct gtccgctgtt
     9721 gtcacaccga aagctaccaa ggttggcggt actgtcaatt cttcgaaagt accagacata
     9781 gagtccttac cgccgattga tggcaatcca agttgaatct gagcctcaat agaaccaaga
     9841 agagctgata ctggctgatc aaaacgctct gcctgtttat ccatacgctc gaaatactct
     9901 tggtaagaga aacgtgcacg agaccagtca gcacccgttg ctaccaagcg agctgtcgct
     9961 tcaatgacag cataggcagc accgtggtaa ggtgaccatt ctgcaatata aggattgtaa
    10021 ccttgagcca taacagatgc agttgttgtg acaccgtgtt gaactggcaa tttttgaaca
    10081 gagctttctg ttggtgtgat ttggtaacgg ccaccgattg ggtggttaac ggttgagcga
    10141 ccaacagatg agtcaaagat agtttggaga cctttttgac tagcgtggtt gaggtcagac
    10201 aagaccttaa gcgtatccgc ttcaagtgtc tctgctgatg ttgtgcgtac ctctggaact
    10261 gtcaagtctt tgtcaacgac tttagcatca acaacgacac ggacaccgtt ggtatcaagg
    10321 aaacggcgtt ccaagtcaac aattgtttca ccatcccaag tcatgacaag gtttggtttt
    10381 gcagtaatag tagcaaccac aacggcatcg atattttcct tgttgcaggc tgcgatgaag
    10441 gtatccacgt cactaggacg aacaacgact gacatacgct cttgtgattc tgagattgca
    10501 atttcagtac cattaagacc ttggtattta agcggtacct tgtccaaatc gatttcaaga
    10561 ccgtcagcca attcaccgat ggcaacacag acaccacctg caccgaagtc atttgatttc
    10621 ttgattaggc gagtgacatt accatcacgg aaaaggcgtt gaatcttacg ttcttcgatg
    10681 gcattccctt tttgtacctc tgcgccagct gtttccacag attcaaccgt ttgaacctta
    10741 gatgaacctg tcgcaccgcc gacaccgtca cgacctgttt taccaccgag caagatgacc
    10801 acatcgcctg cttctggttt ttcacgaacc acattttcct taggtgcagc accaaccaca
    10861 gctccaagct ccatgcgttt ggctacaaag ccagggtgaa agtactcacg cacataagtt
    10921 gtcgcaagcc caatctggtt accatatgaa gaatagccgt gcgccgcagt tttagaaata
    10981 acctgttgcg gcaatttacc agcacgtgtt tccgcaatcg gagtcgtaat atcgcctgcg
    11041 cctgaaatac gcatagcctg ataaacgtat gaacgtcctg acaacgggtc acgaatggca
    11101 ccaccgatac aagttgctgc gccaccaaat ggctcaattt ctgttgggtg attgtgagtc
    11161 tcattcttaa acatgaggag ccaaggctct tttacaccat caacatctac ttcaatttca
    11221 actgagcagg cattgatttc atctgagact tccatatcat ccagtcgtcc attggcacgt
    11281 tcataacgac caaagatagt cgccatatcc ataagtgtct gcggcttttc agaacgacca
    11341 agttcatcac gcatggcgat atatttgtca taagtcgcct gcaattgttt ttggaattta
    11401 gaagctgaaa agtcgatgtt tttcaactca gtttcaaagg ttgtgtgacg gcagtggtct
    11461 gaccagtaag tatccaaaac tttcaactca gtttcagttg gcacacgccc gattgatttg
    11521 aaataatctt ggatgaagag aagatcatcg acctccatag ccaagccctg ctctgcctta
    11581 taggccgcaa aatcgtcagc cttataagtt tcaaagaaat caagactagg gattgtctta
    11641 tcagacacag agaaagcctg ctcttcaagc ggcaaggtaa tgtccttgaa acgtgaatca
    11701 acaggattca aaagatagtt cttaacggct tcaagctctg cttctgcaat atccttattg
    11761 accaagtaaa gctgggctgt attgacctta acctgactgt cacttccaag caatagcaaa
    11821 gcttcttgcg aactagcagc acgttggtca aattgaccag gaagcgcctc aatggcaaag
    11881 aaggcaacct tatcaagctc cgcagtgatt tcagtttccg tcaaaagaca gtctgtcacc
    11941 tgctcagaga aaatatgctt ctcagcacgc gccagcaaat cctcagccaa attgaagaca
    12001 tcatagacct gcacaatacg caaatccttc aaagaagtca gttgtagatt atgcgtcaac
    12061 tctttcacaa gactagccga tttaatgtca aagtcagcct ttttctcaac aaaaatacgt
    12121 ttattcatat aagataccaa gtccgacaag ggagcgactg aaaaatagga ggatatctga
    12181 aattgagatt tcagtaagcc caatcttttt tctgagtttc ccgaacggat tcaattcctt
    12241 cctcttctaa ttacaatgct atcaacttct caagtacaac ttgatagaca tcggtcaagc
    12301 taccaagatc tctacgaaaa acatccttgt ccatatgatt tccttcagca tcccatagac
    12361 ggcagttgtc aggtgagaac tcatcagcca agatgatttt gccatccttg tcaaatccga
    12421 actccaactt gaagtcaatc aagttaagcc caatctgagc aaaccaatct ttcaagagtt
    12481 cattaatacg gcgagtctct cctttcagat aagcaatttc ttcatcatta acaataccca
    12541 aaaacttcat atgctcatca ttgataaaag gatcattcag atcatcattt ttgtagtaaa
    12601 attccacaat tggagtttca agaacgcgtc cttcttccac tccaaaacgt tttgaaaatg
    12661 agcctgctgt cacattccgt aataccacct caagtggaat aatatcaact tttttattca
    12721 attgttcgtt ttttgaaatt tgctcaatat aatgtgtcac cacaccagcc atatttaatt
    12781 tctcaaaaat aagagatgaa atctgattat ttagtgctcc cttgccatca atagtctcct
    12841 tacgagctcc attcaacata gtagcttggt ctttatagac cgttctaatc acattttcat
    12901 cttttgtact ataaatatct ttcgcttttc ctttataaat aagctggtta gtcacttgat
    12961 tattcgcctt tctcttattt taagagttat tctaacacac aacattatct aatacaacaa
    13021 aaactttatt taaaatcaaa aaaaagaaga aacgttcgta ttttcttcct ttttattatt
    13081 acttatgttc aatgatataa tctaatagtt gttggacgct tgacatgtgt tcgacagcct
    13141 catctggaat ctctagatga aacacatctt ccaaattgat aataaactct gttaaagcga
    13201 tagagtcaat tgctaaatca tcatgtaacg atgactctgg tgtgatatct aactgtgggt
    13261 caccaagttg ctgccttaat aattccagca tcttttcaaa aacttcatct cttgacatga
    13321 gttcactcct attcatctgt aaaggcatca actaattgat caactacttg agtttccaac
    13381 attgtacgaa cttgcttcaa agtagaataa acagctttag aatcacttga tccatggcat
    13441 tttactattg gagcttttaa accaaaaaga accgcacctc ccgcgcttga atagtccata
    13501 ctatctttta gctgatacag gctatctttt aatagaagtg ctcctaattt agctttcacg
    13561 ccacctgatt taattgaaga ttttagactt cccataatat tcatagctgt accttccatt
    13621 gtctttagaa cagcatttcc agtaaaacca tcagtgacaa caatatctgc tacactagac
    13681 atcaaatcac gtgcttcaat atttccaata aaattaatag aaggctctgc tgctaacagt
    13741 tcataggctt ctttatgaag agaatcgcct tttgtttctt ccgttccgtt atttaagaga
    13801 cctacacgag gaacttcaat accacggaca ttttttgcat aaaacgaccc taaaatagca
    13861 tattgatgaa gatgagaagc tgtgttttct gcattagctc ctaaatcaag catatcaaaa
    13921 cccacaccat ctaacgtagg catagttgac attaagcctg gtcgatcaac accttttata
    13981 cgtcctacta caaacaaacc agcagctaat aaagctcctg tattccctgc agaaataaag
    14041 gcctgtgcaa caccatcttt gactgcctta gctcctaaga ccatcgaaga ctgtttttta
    14101 cgacgaacag ctttagcagg ctcatcatca gaattaattt tttcttcagt atgaataata
    14161 ctaactcgct ccgttactgt taaatatttc tcaatttttt tctgatcgcc atagagttgt
    14221 acttcaatat cactaaaatc tgaaatagct tgattaacac cctctacaat agctttggga
    14281 gcgtaatctc cacccatagc atctacagca attttcttca tgaatgaact cctcataaaa
    14341 acttagtggc tatattatag catgtttctt tatttttttc acaagaaggc aataatagct
    14401 aatatctaac agatactttc actctttcca aagaaaagta tgctaaacaa cctactaata
    14461 tcaaagaaca tagtactccc ccttcaaaac caaaatcccc accactccaa atagcaaagc
    14521 tagaattttc agtaattcta aaaagagaaa caggagaata ccccccacta accttattac
    14581 ctaatacaca tcccatagta aaattccatg cagtatgaac tcctgtaaca aaaagaatat
    14641 ttttggtcat attaaaaaga atagaaaaaa taataccaag taaaaataaa ttaaaaagtg
    14701 ctattgctgt gataccatag tttctaaagt ggataaagga aaacaacata gaattgcata
    14761 aaacacctgc aaaactacct ttaaatttgg taactgttgt cataacatat cctctaaata
    14821 aaatttcctc tgtcaagctt tggataaagt atgcaagtaa taaaaagaaa aaaggtccaa
    14881 aagaaaagga attgctggaa gaaatttcca caaaacctga taaccagtgg caaatataaa
    14941 taaaaaacat cagtaaacta ccagtaataa atcctgagat cgttgttaaa acattattat
    15001 ctttagtaaa tcccaaggaa gtaaaatccc ttttttgaaa atagattaaa aatagtaaaa
    15061 tacttgccaa aataggaata aatagtaaaa gcgcaatgta taaattgaat ttataatcat
    15121 caggtagcaa ttttactaca aggcgtctac ttacaatgat gccgataact tgaattagaa
    15181 ctgcaccaat aactggcaca aaccagttag caaaaaagag tttattggta atgtataatg
    15241 tttttttcat ctcaaatatc ctaaaaatta attccatatt agtttatcct aatattaaaa
    15301 aaaagaacat gacactaatg tcatgttcct ctttacaact taatctgatt tcataatact
    15361 tccccaacta gacaaatcat caataaattt tttactttta agatgaatcc ctacatattc
    15421 atcgtaaatc atgtccaaaa attgacgaat tttaagtttc atctcaggtt tcacagaaat
    15481 tgtttgtaaa tcatcaaatt gaattgactg aaaacgattg attaagtaca gcatattagg
    15541 atctaggtgg tttcttctct cgtctttata ataatggttt ggacataata atccagaata
    15601 tttgtgtgaa aaatcaaaag gtaatcctgc cctatgacaa aaaacacaat cataaaaatt
    15661 taaagcaaca ccgaacctct ctagtagttg tacttcaaaa atattcgtta gaatctcata
    15721 atctaaaccg tcctcaatca attctaacgt tttctttagg aaaataaata attgagcatc
    15781 cgctacatta tcactaatag ccacatcagc caaagaagta atataactcg catatgacaa
    15841 cttaaacaag tctgaattgg tttcttgaaa tgctaaaact tctttataat catcaatata
    15901 agaaagacca ttatcattaa tttttaaaat aaaatgagca atagtcaaag gttggagcac
    15961 agcattgaat tttgactttg aagcatgctt tacaaaaaac attcttttcc cctcagtctc
    16021 cgtaaagatt ttaactaatt tatcgtcttc acgataatta cgattataca aaacgagacc
    16081 gtatgtttga ctaaccctca tatgattcca taaaaacttt caatctagcc attgctgttt
    16141 caataacttc catactagca gcataagaaa gacgtatata cccttcacca tatttaccaa
    16201 aagccacccc tggaataata gctacagctt gttgataagc aaaatcctga caaaacttga
    16261 aagaatcctg tggataacta tcaggtattt tagcaaagat ataaaaagcg ccgctcggtt
    16321 taattatttt gaaaccaagt ttagacattt tatcaatgat ataatctcta cgtttaatat
    16381 atccttgacg cataggtaac gtatcatttt tacccacaga taaggcttct accccagcaa
    16441 attgcgaaat agtactagca gctgtcacca tgtattgatg acttttaatg atttgagcag
    16501 tagatgcttc aggagcatac accaatccaa ctctccaacc agtcatagca tgtgattttg
    16561 ataagccatt aattaagata gtttggttag gtaaatattc tgcaattgaa acatgttgct
    16621 gaccagtgta agttaactca gagtaaactt catcactaat aacaaaaata tcatattttt
    16681 taaggacttc agctaatgca gcaatttctt gccttgaata ggtaatacct gtaggattag
    16741 taggataatt aagtaaaaca gcctttaatt tttcaccttg ttggataata gccgtttcta
    16801 acatttcagg ggttagtcta aagtcatttt ctctagtatc aatttctacg atatcagctc
    16861 ctacaaggtt aactattggc tcatagcctg gataagcggg ggcaggtaat agaacaacat
    16921 caccagcttc taaaatagct ataagagatg cagacagagc ttctgtggca cctatcgtaa
    16981 ccaagatctc actatcaggg ttataagtaa gattatattt atcctttgca aaatctgcag
    17041 cagcttgcct tagagctaat aaaccgctca taccagtata atacgattga ttcgcatcaa
    17101 tagctgattt agctgcttct ttaacatgat caggagtggt aaaatctggt tctccaagag
    17161 ttaattttac cattccaggt atatctgaaa tagattgatc aaactgacga attaaggaga
    17221 cctctattct atctaagtac ttattaaatc gtttctctaa tgtcatcata acctcctgat
    17281 aagaaataat atatttaatt atacaagaaa aataatttgt gtccagtttt ccgtcattaa
    17341 atgataaaaa acctgttaat aacaggtttt ttatcatttt agtttattca tttcaaataa
    17401 tggtgataaa ggacgtttct cgtgaatacg gataatagct tcaccaatca attccgcaat
    17461 tgaaatttgt tcaatcttat caatcaaacg ttcttcaggg aggtaaatcg tatctaacac
    17521 aattaatttt tcaatagctg aattttgaat attatccaaa gccggccctg ataaaacagg
    17581 gtgagtacat gaagcataaa cagctgttgc ccctgcttca gctaaagcgt ctgcagcatg
    17641 gcaaatagta cctgcagtat caatcatatc atcaattaaa atacattttt tacctttgat
    17701 attaccaata atattcatta cttcagatgt attcatttta gtaacactac ggcgcttatc
    17761 aatgatagca atcggtgtct ttaaacactg tgccagctta cgtgcacgtg ttacgccacc
    17821 atgatcagga ctaacgacaa caacatcatc accaactaat ccctgacgat caaagtaatc
    17881 tgcaataagg ggagccccca taaggtgatc aacgggaata tcaaagaatc cctgaatttg
    17941 agctgcatgt aaatcaactg ttaaaagacg atcaacacca gcaacttcta gcatgttcgc
    18001 aacgagtttc gaagtaatag gttcacgaga acgtgccttg cgatcttgac gagcataccc
    18061 atagtaaggc attaccacac taacagactc cgcactagca cgctttaaag catcaaccat
    18121 aattaaaatt tccatcaaat tatcattaac tggtgagctt gttgattgta aaataaaaac
    18181 atgatggcca cgaatagact cttcaatatt aacttgaatc tcaccatcag aaaattggcg
    18241 aactgtcgat tgacctagcg gaattccaat cgtctgagac acttttttcg ctagttcttt
    18301 gtttgatgac agtgcaaaca attttaaatt agaataagac atgatttcct ccaaaacttt
    18361 agtcctatac cattttaacg ctttttacac aatttttcaa tccaaaataa accataccta
    18421 attaataaac aaaaaggaga ccacgaaaga tctccttagt attcattaat ttgggtaaat
    18481 atagtaaaca gagccagaag cactagggtt aaatgaacca cggtaattgc caatgctcat
    18541 atttccagca tagtttgatt ccataacttg gatagatgaa ttgttagcca ctgacgttac
    18601 tactgcaacg tgcccgtaac caccaccatc atatggccaa acagctactg caccaacacg
    18661 aggggtagtt cctacagaat aaccagctgc acgtgcacta gcaccccact gattagcatt
    18721 tccccaatag ttaccaaccc aagaagccat tgacttagct ccccaagtac attgtcccat
    18781 tgggtatgtg ttacttgaat cataacttct gacaacaggt tttgataccg ctctaggtgt
    18841 tgaagaaaca actgctttag gtgcttcaac tgctttagtt actacagaag gttgagtaac
    18901 ttttggctca ttagtagctg tagctgtagt tttagcaaca gtagttgccg aagaagtagc
    18961 cgtaagatta gaaggcttta tttcagttct cggttgtgtt tgaactgttt cagtaggagc
    19021 tgtggcagac tcaacttgtg ctgctgcctg tgcttttgct acagactcag cttgtgcttt
    19081 cgcttcagct actgcttgtg cttcagctgc cttacgcgca gcttcctcag cttgtgcttt
    19141 tgcctgaatt aaagatgctt tttcattttg gactgtagtc aattgagcag ataactctaa
    19201 ttgagctgct tctaattgtg ctctttgtgt tgctaaggct gctttattat tttcgatagc
    19261 ttgcttgtta gctgctacag tatttatagc attttgattt tcaatttgtt tagcttctaa
    19321 ggctgctttg tcagcctctt gttgtgctaa catcttttca ttagctgaaa caacctcacg
    19381 aatagcaaca acacgattaa cagcatcaga tactgatttc gaatttaaaa tagtattaat
    19441 atagttagtc aagttaccct tttgagcact acgaacttgc tttttcaaag attcgttacg
    19501 tgcaacaatt ttattagaaa gagtttgaat ctcttgacct aactgctgag atactgcctc
    19561 aagttgagca ttttgcgctt ctaattctga ttgttgagac tctaaagcac caacttgtcc
    19621 tttaatagca gtcacctggt tttgtgctgc tgcttgttgc cctgataaag tattgattac
    19681 tgaatcagta gcagcaattt tcgagtcaaa gtcatcagcg tttactgtta cggcagcagt
    19741 tccaagagtt actccactca caaggactgc tgataatatt ctttttttca tttttattaa
    19801 actactcctt tacgataaga cattaaatat tttaccaaaa aattcacgaa attatattac
    19861 gtcattgtta catttatatt tgaaatcaac tatttctaaa tgaaccataa tcaaatctag
    19921 aaaacgataa ccttcttcta ttcactctat caatataatt actccatagt gaaactaaaa
    19981 gagaaataaa aaaagagtat aattactctt aaaattataa tatttacgga gaataaggga
    20041 ttcgaaccct tgcgccagtt acccgaccta acgatttagc aaaccgtcct cttcagcctc
    20101 ttgagtaatt ctccaaatta atattaatgg gcacgagtgg actcgaacca ccgacctcac
    20161 gcttatcagg cgtgcgctct aaccacctga gctacgcgcc caagcaaatg cttggtttta
    20221 cttttatgta aagtaagcgg gtgacgagaa tcgaactcgc gacaacagct tggaaggctg
    20281 tagttttacc actaaactac acccgctaaa aacttatat
//
LOCUS       DATRNX010000032        19525 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRNX010000032 DATRNX010000000
VERSION     DATRNX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19525
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..786
                     /locus_tag="VBC81_000912"
     CDS             <1..786
                     /locus_tag="VBC81_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911962.1"
                     /translation="TTSTILKPFEQKKIVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQ
                     EKTKSPTPQKEAVKEQTEQKISGKTQNVEKKSETVATPQQSSTAQTSVQQPAPVQPVV
                     QESKASQEEINAAHDAISAYKSTVNIANTAGVTTAEMTSLINTQTSNLSDVEKALGNN
                     KVNNGAVNVLREDTSRLENMIWNRAYQAIEEFNVARNTYNNQMKSETVPVDNDIEAIL
                     AGSQAKISHLDNRIGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            853..1398
                     /locus_tag="VBC81_000913"
     CDS             853..1398
                     /locus_tag="VBC81_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6911963.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1549..1638
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1794..2360
                     /gene="thiW"
                     /locus_tag="VBC81_000914"
     CDS             1794..2360
                     /gene="thiW"
                     /locus_tag="VBC81_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6911964.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2357..2911
                     /locus_tag="VBC81_000915"
     CDS             2357..2911
                     /locus_tag="VBC81_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6911965.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            2915..4201
                     /locus_tag="VBC81_000916"
     CDS             2915..4201
                     /locus_tag="VBC81_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6911966.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4189..4857
                     /locus_tag="VBC81_000917"
     CDS             4189..4857
                     /locus_tag="VBC81_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6911967.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            4941..5621
                     /gene="tenA"
                     /locus_tag="VBC81_000918"
     CDS             4941..5621
                     /gene="tenA"
                     /locus_tag="VBC81_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6911968.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5623..6420
                     /gene="thiD"
                     /locus_tag="VBC81_000919"
     CDS             5623..6420
                     /gene="thiD"
                     /locus_tag="VBC81_000919"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6911969.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6422..7192
                     /gene="thiM"
                     /locus_tag="VBC81_000920"
     CDS             6422..7192
                     /gene="thiM"
                     /locus_tag="VBC81_000920"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6911970.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7206..7877
                     /locus_tag="VBC81_000921"
     CDS             7206..7877
                     /locus_tag="VBC81_000921"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6911971.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8004..9263
                     /locus_tag="VBC81_000922"
     CDS             8004..9263
                     /locus_tag="VBC81_000922"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6911972.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9365..9919
                     /locus_tag="VBC81_000923"
     CDS             9365..9919
                     /locus_tag="VBC81_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6911973.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            9912..11195
                     /locus_tag="VBC81_000924"
     CDS             9912..11195
                     /locus_tag="VBC81_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6911974.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11218..12078
                     /locus_tag="VBC81_000925"
     CDS             11218..12078
                     /locus_tag="VBC81_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6911975.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLAKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12080..13000
                     /locus_tag="VBC81_000926"
     CDS             12080..13000
                     /locus_tag="VBC81_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6911976.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13017..13472)
                     /locus_tag="VBC81_000927"
     CDS             complement(13017..13472)
                     /locus_tag="VBC81_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6911977.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13543..13656
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13654..14163
                     /locus_tag="VBC81_000928"
     CDS             13654..14163
                     /locus_tag="VBC81_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6911978.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELTSLLVGAVLVDK
                     LSRHVDFTK"
     gene            14303..16261
                     /gene="ligA"
                     /locus_tag="VBC81_000929"
     CDS             14303..16261
                     /gene="ligA"
                     /locus_tag="VBC81_000929"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6911979.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16273..17292
                     /locus_tag="VBC81_000930"
     CDS             16273..17292
                     /locus_tag="VBC81_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6911980.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            17296..>19525
                     /gene="pulA"
                     /locus_tag="VBC81_000931"
     CDS             17296..>19525
                     /gene="pulA"
                     /locus_tag="VBC81_000931"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6911981.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISN"
BASE COUNT         6349 a         2956 c         3973 g         6247 t
ORIGIN      
        1 acaacttcta ctatattaaa accgtttgaa cagaagaaaa ttgtggaaga ttttaatcct
       61 tattctaatt tagacaattt agaaataaaa aaaataaggt tgaatggctc ccaaaaacaa
      121 aaagtagaac aggaaaaaac taaatcgcca actcctcaaa aagaggctgt aaaagaacaa
      181 actgagcaaa aaatatctgg gaagactcaa aacgtagaaa agaaatctga aactgtggca
      241 actccacaac aaagttcaac tgcgcaaact tctgtccaac agccagctcc ggttcaacca
      301 gttgttcaag aatctaaagc ttctcaagag gagattaatg cagcacatga tgccatttca
      361 gcgtataaat caacagtcaa tattgctaat acagccggtg taacaactgc ggaaatgacc
      421 tcgctcatta atactcaaac ttctaatctt tctgatgttg agaaagcttt agggaataat
      481 aaggttaata atggtgcagt caatgtattg agagaagata catctcgtct tgagaatatg
      541 atttggaatc gtgcttacca agctattgaa gaattcaacg tcgctcgtaa tacttataat
      601 aaccaaatga agtcagaaac agttccagtt gataatgata ttgaagctat tttagcaggt
      661 tctcaagcta aaattagcca tttggacaat cgtatcggag cgcgccacat ggatcaagct
      721 tttgtagcta gtttattaga agttactgag atgagtaaat caatctcatc gcgtataaaa
      781 gaatagacac tgctatcaag gcgatcttaa acttttgtat tgagctaacc taaaagatag
      841 aaagagacta atatgaaaaa aataacaact ttaatcttag ctagtagctt attactagtt
      901 gcaacgacat cggttaaagc tgatgataac tttgaagtac caacgcgtta tgttaaaatg
      961 agtgaaaaat caaaagcatt ttatcaaaga ttacaagaaa aacaacgtaa aacacatact
     1021 actgtgaaga cttttaataa ttcagaaata aggcatcaac tacctcttaa acaagaaaag
     1081 gctaggaatg atatctacaa tttaggcatt cttatttctc aggagtctaa agggttcatc
     1141 caacgtattg ataatgccta ttctttggaa aatgtctcag atattgttaa tgaagctcag
     1201 gctttgtata aacgcaacta tgatttattt gaaaaaatca aatctacacg tgataaggtt
     1261 caagtcttac ttgcatcgca tcaaggtaat acagacttaa aaaactttta tgctgagttg
     1321 gacgatatgt atgaacatgt ttatctcaac gaaagtagag tggaggcgat aaacagaaat
     1381 atccaaaaat ataattagtt tctaaactaa caaacattcc taaatataag acattaaacc
     1441 ctacttattg attagtgagt agggttttac tgttttaaat agctttctgc tcagaatgta
     1501 agccttgtca tttcaaagga actatgttat tattcttaag taaattaaat aggacatttg
     1561 gggtgcgtaa cagctgagat tatacccatt gaacttgata cagttagtac tgtcgaaggg
     1621 aaatgtgtgc cattacaagt tttttgtaat ggaaaagggc gtagtgtctt cttaggagac
     1681 agctagctct agttaatcag ataataaact ccgtttgctt gcagatggag ttttttgtcg
     1741 tcaaaatttt ttataggttg ttgcccctta tctgtcatag agaaggagtt atgatgacta
     1801 atcataaaac aaaaaaatta acggttttag caattatgat tgctttagat gtttttctaa
     1861 cgcccatttt tagaatagaa ggcatggcct ttatgtctag tgtggttaat attatcgctg
     1921 gagttataat gggaccaatc tatgcattag ttatggcact tgtaacggct tttattcgta
     1981 ttttaactca agcaggagta gcatctgttg cacctttagc tattctagga gctggtccag
     2041 gagctttctt agcgggttgg ttttatcggt tttatgcgaa agactggatg tcttgggtgg
     2101 gtgagtttat aggaacaggg ataattggag cacttttatc agctccaatt atggtctggt
     2161 tttggacttg gacagcggat ggaaatcacg aagtgttagc taaagcatct gcagctcaat
     2221 ccatttttct ttttatgcca aggtttatag gggcgactat aattggcggt gttattgctt
     2281 tgttgctact tcagggatta aagcgggttc ctgtatttat gacaatacaa gcattattta
     2341 aaggagaaaa agtaaaatga aattgggttt aaaagatgta ttattgattg ctttattagc
     2401 ggttgttctg ggcgttgttt actttggagc aggctatatt tcaaatgctt ttgtaccttt
     2461 tgtaggtcct atagcgcatg aagttattta tggtatttgg tttgtggcag gtccaatggc
     2521 tctttatata ttgaggaaac caggaaccgc tattgtagca gaattgttag ctgctttgat
     2581 agaggtttta attggatcaa tttatggacc aagtgtactc gttattggta cccttcaagg
     2641 tttagggtca gaattaggct ttacgctgtt taggtatcat aattacaagt tgccagcttt
     2701 tatattgtct gctattttga catcaatttt tagttttgca tggagttttt atgccaatgg
     2761 acttagtgca ttttcatttt catataacat tctgatgctt atagtaagaa ctgtttcatc
     2821 tattattttc ttcttattaa caaagaatat atgtgaccaa ttgcatagaa gtggtgtact
     2881 gaatgcttat gggattcatg aagggaagta gcctatgcta tctgttgaaa aattagctta
     2941 cacccatggc gatagtcatt atttatttga tgaagtgact tttagtctta acccaggaga
     3001 acgaatttta attagtggct attcagggtg tggtaagagt acattggctc ttttactatc
     3061 agggttaaaa gaaagtggga aaggtcaagt tttattaaac ggctcattaa tagaaccaag
     3121 cgatgtgggt tttctttttc aaaatcctga tttgcaattt tgtatggata ctgtggcgca
     3181 tgagttgtat tttattttag aaaatttaca aatagaaccg gagcaaatgc aagatagaag
     3241 tgagtttgta ttggcacaag ttggtttaaa aggatttcag aatcgtttga tttatacttt
     3301 atctcaaggt gaaaaacagc gattagcatt ggcgactatt tttttaaaat ctcctaaact
     3361 tattatttta gatgaagctt ttgcgaattt ggatcaagag agtgccagtc aactattaca
     3421 attagttctt aattatcaag caaacaatca atcaatgctg attgtaatcg atcatttgat
     3481 tacttattat caagatataa tggatcacta tttttggttg gaaaaatggt tgataagggt
     3541 cgattttgat tatatgctca atcgtttgaa tgtcttcgaa ttagaaaaga aaagtcataa
     3601 tacaggtgac aaactgttat caattaaaga tttccaagtt aaactttcaa aaaacaaatt
     3661 catctcttac cttgattttg atttagcaag tggggagagg ttgtgcttag atggaccaag
     3721 tggcgtgggg aagtctagtt tctttatggg attgttaggg ctctatagga ctaaaggaaa
     3781 gaaacaattt actcatagaa agcaaattcc tataagcttt ttatttcaaa atcccttaga
     3841 tcaatttatt ttttcaacag tttatgacga aatttttcag gtttgcaagg atagtaacaa
     3901 agcgagggat attttggaaa ccataaatct ttgggataaa aaacagtttt caccatttca
     3961 actaagtcaa ggccaacaga ggcgattagc cattggcagt attttagcta gtgatagtaa
     4021 acttctatta ttagatgaac caacttatgg acaagatgcc tatcacgcta atatgattac
     4081 gactcttctt ttgagttatt gtcacaaaaa tcattgtggg gttattttta ctagccatga
     4141 tcctcactta aaagagtgtt ttgcaacacg tttcttggag gtgtcatctt gttcaatttg
     4201 aaaaactatc atccagctat caccttccta tgtttattaa taccaacctt ttcgtttagt
     4261 ttcacattga ggctaagaac gtctttgtta tttctaatta tagtagtaac actgcaatgc
     4321 ttcgtaaagg tttctttaaa aacttgggcg aaggtcaatt tgatctcatt tgtgatggga
     4381 ctctctttat ttttagggac gtatttttgg gggaaattac cccatcaatt cgtcttagct
     4441 agtctagtgg cctgtcgtcc gcttattttt atgaacgtgg gtttgttatt tcatgcatca
     4501 cattctaatt atgattttat agaatctctt tatcaaactt tcaaagtgcc tagtcatttt
     4561 gcatatggta tttttgcggt ctttaatctt ttaccattaa ttaaattaca ataccaacgt
     4621 aatcgactcg cattccgact gaagaatcag gtaacttggg cattatcgcc tagactaata
     4681 ttgtcagttt tattaaagac gatatattgg gtagaacagt tagagttagc aatgctttct
     4741 aaaggttttg aagtgggtaa agagcgaaca catgcttcaa cttatcccgt tcgttttcga
     4801 gattatagtt tacttggtat gagtatttta ttggctatag gaatgatttt taaataagta
     4861 caattagttt agtaatacaa ttgtgcattt atatagagtg agcatgttct gctcatgaga
     4921 tcataacaaa ggagattact atgttaaaag atgagatgat tgctaaagga acaaaatata
     4981 tacaatccat ctatcaagat ccatttattc aaggaattgt aaaagggcgt ttagaccatg
     5041 acgtaatctg ccattattta caggcagata atatctacct tgggaaattt gctgatattt
     5101 atgccctgtg cttggcaaaa tctgataatt tacgagataa acaatttttc cttgaacaag
     5161 ttgactttac attaaatcgg gagttagcag atggtgaagg tccacatcaa gccttggcag
     5221 cttatactaa tcgatcttat caggatatta tcgaaaaggc tgtatggtac ccgtcagcag
     5281 atcattacat taaacatatg tattttcatt tttacgaaaa tggtattgct ggtgctttag
     5341 ctgcaatgag cccatgtcca tggatttacc atcaattggc taaaaaaata attgaggaga
     5401 accagttttt gaatggtaac ccttttaata attggataac tttttatgct aacgatacag
     5461 ttgaagagtt aatggaaaac tattttcgta tgatggatta ttatgcgcag aatttgtcaa
     5521 aagagaagca agctgatttg gtagatgctt ttgtgaaatc ttgtcagcat gagagacgtt
     5581 tttttcaaat ggcaataaat caagaaaaat gggaggatta atatgactta tttagcacca
     5641 gttttaacta ttgcaggaac tgatcctagc ggtggagcag ggattatggc agatttaaag
     5701 acatttcaag ctcgtcggac ttacgggatg gctgttgtga ctagtgttgt tgctcagaac
     5761 acttgtgggg ttagaggcgt tcagcatata gaaacagcta tcattgatca acaactagct
     5821 tgtgtttatg atgatattaa gccaaaggcg gtcaaaacag gaatgttagc ggagagggaa
     5881 acgattagtt tagtcgcttc atatttaaaa aaatatccgc aaccttatgt attagatcct
     5941 gttatggtgg caactagcgg acatcgttta attgattcag atgccgtaga agcattaaaa
     6001 gaagacttat tgccattagc aactattatc acaccaaatt taccagaagc tgaggtttta
     6061 gtgggatatg acttatcaga tgaagtaagt attattaaag caggttacga tattcaaaaa
     6121 caatattctg ttagaaatgt tcttataaag ggaggacatc tagatggttt ggctaaagat
     6181 tatttatttt tggaaaaaga agggttgata actcttagta atcaacgtat caatactatt
     6241 catactcatg ggactgggtg tacatttgca gcagtcgttg cagcggagtt agctaaaggg
     6301 caatcaattc taaacgcggt aagtaccgct aaatcattta ttacttcagc tatcgaaaca
     6361 gcgcctgagt tgggtcttgg aaacggtcct gtcaatcata ctagctatca aggagactaa
     6421 aatgtattta gaacaactaa aagaggtaaa tcctttaacc atttgtatca ctaataatgt
     6481 ggtcaaaaac tttacagcca atggtttatt agctttagga gcttctccgg ctatgagcga
     6541 gtgcatagag gacttagaag acttgttaaa agttgcagat gcattattaa ttaatatagg
     6601 aacactaaca aaagaaagtt ggcaacttta ccaagaagca atcaaaatag caaataaaaa
     6661 ccaagttcca gttgtattag atcctgtagc ggcaggagca agtcgttttc gattagaagt
     6721 gtcacttgat ttattgaaga attatcctat tagtttactt agaggaaatg gtagtgaaat
     6781 cgctgctttg gttggtgaaa aacaagcgtc taaaggggca gatgggggaa aagtggctga
     6841 tttagaatca attgctgtaa aagccaatca agtttttgat gtgccagtgg ttgtcacagg
     6901 agaaactgat gctatcgctg ttagaggaga agttcgctta ttacaaaatg gtagtccttt
     6961 aatgccactt gttacaggaa ctggatgtct attaggggca gtattggctg cttttattgg
     7021 tagttctgac agaagcgatg atcttgcttg tctgacagag gccatgactg tttataatgt
     7081 tgctggagaa attgcagaga aagtggcaaa aggtaaggga gtgggaagtt tccaagttgc
     7141 atttttagat gccctttctc agatgaaatc agaaatgatt atggacaaat aggcggtgtc
     7201 agtagatgaa agatacattg aaattatatt ttgtttgtgg gactgtggat tgctctagga
     7261 agaatatatt gactgttgtt gaagaagcct tacaagcagg tataactcta tttcaattta
     7321 gagaaaaagg ttttacagca ttacaaggta aggaaaaaat tgctatggct aagcaactcc
     7381 aaatactttg taagcaatat caagttcctt ttattattga tgatgacatt gacttagttg
     7441 agttaattga tgctgatggt cttcatattg gacaaaatga tttacctgtt gatgaggcac
     7501 ggagacgttt accagataaa atcatcggtt tatctgtgag tacaatggct gaatatcaga
     7561 aatcacaatt atctgttgtt gattacatcg gaattggtcc ttttaatccg acacaatcaa
     7621 aagcagatgc taagcctgct gttggcaata ggacaacaaa ggcagttaga gaaattaatc
     7681 aagatattcc aatcgttgca ataggcggaa tcactagtga ttttgttcat gatattatag
     7741 aatcaggcgc agatgggatt gctgttatat cggctatttc aaaagcaaat catattgtag
     7801 atgcaaccag acaattgcgc tatgaagttg aaaaagctct ggttaatagg caaaaacaca
     7861 gtgatgtaat caaataaggc tacatcgtta attataagaa tttttaagat attatatcat
     7921 cacacaatac tggtatttga aaaagaattt aaactatgat aatatagtag agttgaattc
     7981 tagttaaaga aagatttgaa agtatgcgta aaattattat taacggtggc aagcaactga
     8041 cgggagaagt tgctgtatcg ggtgccaaaa atagtgtagt tgccttgatt cctgccacta
     8101 ttcttgctga tgatgttgtg gtcttagatg gagttccggc tatttctgat gttgacagtt
     8161 tagttgacat tatggaaact atgggagcta agattaagcg ttatggtgag acgcttgaaa
     8221 ttgatccacg tggcgttaaa gatattccaa tgccttatgg caaaatcaat agtcttcgtg
     8281 catcctatta tttctatgga agcttattgg ggcgttatgg tcaagcaaca ttaggtttac
     8341 ctggtggttg tgatttaggc ccccgtccga ttgatttaca tttaaaagct tttgaagcga
     8401 tgggagctag tgtttcttat gaaggtgatt caatgcgtct agcaacaaac ggaaagcctt
     8461 tgcaaggagc aaatatttat atggatactg tcagtgttgg tgcgacaatt aacactatga
     8521 tcgcagcagc taaggcaaat ggtcgtacgg ttattgaaaa tgctgcgcgt gaaccagaaa
     8581 ttattgacgt ggctactctt ttaaataata tgggagctca tattcgtggt gctgggactg
     8641 atgttattac aattgaaggt gtgaaatcgc tccatggcac gcgtcatcaa gtaattccag
     8701 atcgtattga agcaggaact tatattgcta tggcagcagc tatcggacga ggaataaaag
     8761 tgaccaatgt gttatatgaa caccttgaga gtttcattgc taagttagat gagatgggag
     8821 ttcgaatgac agttgaagag gattcaattt ttgtcgaaga acaagaaaag ttaaaagctg
     8881 tatccataaa aacctcacct tacccaggat ttgctacaga tttacaacaa cctctaaccc
     8941 ctttattact aacagcagaa ggaaacggga gtttattaga tactatctat gagaaacgtg
     9001 ttaatcatgt tcccgaatta gcacgtatgg gagctaatat ttctacccta gggggcaaaa
     9061 tagtttatag tggtcctaat caattatctg gtgcacctgt aaaagcgaca gatttgcgtg
     9121 caggcgctgc tttagttatc gctggcttaa tggcagaggg acgaactgaa attacgaata
     9181 tcgagtttat tttacgtgga tattctaata tcatcgagaa actaacatca ttgggtgcgg
     9241 atatccaatt ggttgaggaa tagtatatat aacaaagagt tgagaatatt tcttagctct
     9301 tttcttcttg ttttagatta agtcacttat aggtatttga aatctcttgt ggtataatat
     9361 tatcatggat atatggacaa atttaggacg gtttgcattc attgaaacag aacatgttaa
     9421 cctacgtcct gttgcctaca ctgataggga agctttttgg cgaatagctt caaaaaggac
     9481 aaatttacaa tttatttttc ctgttcaaac cagtaagaag gaaagtgatt ttcttttagt
     9541 tcattctttt atgaaagagc cgttgggagt ttgggcgatt gaagataaag ttagtcataa
     9601 aatgtttgga gttatccgtt ttgaaaacat tgatttatct aaaaagacag ctgaaattgg
     9661 ctattttttg aaagaaagtt cttggggtca aggcattatg actgaatgtc taaagacact
     9721 ttcttttttt gcttttcaag agtttgggat ggacaaattg atcatagtaa cacataaaga
     9781 gaatattgct agtcagaagg ttgctttgaa ggcgcatttc aaacaatcta ggagttttaa
     9841 aggaagcgac cgttatacac gccgtataag agactatatt gagtttcaat taactagggg
     9901 tgattatcgt tatgagtaaa catcaagaaa ttttggagta cctagaaaat ttagctgttg
     9961 gtaagagggt tagtgtacgc agtatttcaa atcatttaaa ggtgagtgat ggcactgcat
    10021 accgcgctat aaaagaggca gaaaataggg gtattgttga gactagaccg cgtagtggga
    10081 cagtacgtgt tgctcaaaaa gcaaaggtta atattgaaaa actgacttat gctgaaattg
    10141 caaggattag tgactcacaa gttgttgcag gaacagaggg gctcagcaag gagtttagta
    10201 aattttctat aggtgcaatg actcacagaa atattgaaaa atatttggtg caaggagggt
    10261 tattgattgt tggcgaccgt gatgaaatac aacatttagc cttacaacat caaaacgcta
    10321 tcttagttac aggtggcttt aatgtttccc catctgtttg tcgtttagca gataaacttc
    10381 aaattcccgt aatggtaaca cactatgata cattcacagt atcaacgatg attaatcaca
    10441 ctttgtcaaa tgccaaaatt cgtactgatt taaagaccgt tgaacaagtc tatcagtcgc
    10501 aaatggatta cggtttctta gcgcaggatg ataccgtaaa agaatttaat cttcttgtta
    10561 aacaaactaa aaatgttcgt tttccaatag tcaaccaagc taatgttgtc gttggtgttg
    10621 ttagtgtgca agatattctt ggaaaagata aagaagtaaa attggcaaca gtcatgtcaa
    10681 aaaatatcat tgttgctaaa cctcgaatga gtctagctaa tatcagtcaa aaaatgattt
    10741 ttgaagattt gaatatgatg ccagtagtat cggatgactt tgaattatta ggcgttatta
    10801 caagacgaca agctgtagaa aatttatcga tgtcgcaagg gacagcttta tacacctata
    10861 gtgatcaaat cttgtctaat ttacaaattg aagatggtca cttttcgttt ttagtagaac
    10921 ccgctatgat tgaccataca ggtagcttaa cacaaggagt tttgacggag ttcttaaaag
    10981 aaatttgtat tcgtgtctta actcgtaagc atcaacgcag tattgttgtt aaacaaatga
    11041 cactttattt cttgcaacct gttcaaattg atgaaataat aatggtaacg ccaacaatta
    11101 tttcagaaaa acggcgcgaa gctacactcg atttagaatt aaaattagaa aataaaatta
    11161 ttgctaaagc tatgatagcg gtaaaaatta attagatata aggagaataa atagaaaatg
    11221 attacattaa agtcagcacg cgaaattgaa gcaatggata gagcaggtga ttttttagcc
    11281 agcatccaca ttggacttcg tgatattatc aaaccaggag ttgatatgtg ggaagttgaa
    11341 gaatacgttc gtagacgttg taaagaagaa aacgtattgc cattgcaaat cggggttgat
    11401 ggtgcagtta tggattaccc atatgctact tgttgtgggc ttaatgatga agtggctcat
    11461 gcctttcctc gtcattatac cctaaaacaa ggtgaccttt taaaagtaga tatggtttta
    11521 agcgaacctt tagctaaatc aattgttgat gtttcatcgt taaactttga caacgtggct
    11581 caaatgaaaa agtacacgga aacatatagt ggtggtttgg cagactcatg ttgggcttat
    11641 gctgtaggtg aagtttcaca agaggtgaaa gacttaatgt ctgtgacaag agaagcaatg
    11701 tatattggga ttgaaaaagc agttatcgga aaccgtattg gtgatattgg agctgctatt
    11761 caggattatg cagagagtcg tggttatgga gttgttcgtg atttggttgg tcacggtgtt
    11821 ggtccaacaa tgcatgaaga gcctatggtg cctaactatg gtacagcagg tcgaggtctc
    11881 cgtcttcgtg aggggatggt tttaaccatc gaaccaatga ttaatactgg tacttgggaa
    11941 attgatacag acatgaaaac tggttgggca cataaaacct tagatggtgg tctttcttgt
    12001 caatatgaac atcaatttgt tatcactaaa gatggtcctg ttattttaac gagtcaagga
    12061 gaagaaagaa cttactaaaa tgaaattaaa aaaattcttt gaggatttgc tggctaaact
    12121 agaatataga cccattcaag tttttatgcg tcattttcaa agtgcagaga tggacttatc
    12181 agccatagca gttgcttatt atctattagt gacagctttt ccattactag ttattgctgc
    12241 taatattttt ccttattttc atattaatgt gtcggattta ttgtcactta tgcaaaaaaa
    12301 tcttccgaaa aatatttatg aacctgcctc acgcttagca gtagatgctt tttcgaaacc
    12361 gtctaccggc attttgggat tcgcgtcatt aacagctttt tggacaatgt ctaaaagttt
    12421 gacctctctt caaaaagcca ttaataaagc atacggtgtt gaccaacaca gagactttgt
    12481 gattagtcgt ttagtgggtg tggggactgg gctgatcatc ctatttttac tgacttttgt
    12541 tttaattttt tcaacctttt caaaaccagt tttacagata attgtaaaca tgtatgattt
    12601 gggagatact ctaacggcgt ggcttttaaa tcttgcacaa ccagttacat ttttgactat
    12661 atttttaggt attggaattt tatactttat tttacctaac gcacgaatta gaaaagtacg
    12721 ttatgtcatt ccagggacat tattttcaac gtttgttatt ggttttttta gtaatttaat
    12781 tagccaatac gtcttaaata gggttgaaaa aatggtagat attaaaacgt ttggttcggt
    12841 cgttatcttt atcttaatgc tatggtttat tttcttagcc cacattatga ttttaggtgc
    12901 aatactgaat gccagcgttc aagaaattgc gacaggaaaa attgaatcaa ggcgtggaga
    12961 tattatgtct cttatccaaa aatccaaaga agaaaaataa gaaaagacag ctcttgttag
    13021 agttgccttt ttttgtcttt aaaaataaca aatttgctca agatgtagtt gagtaaaata
    13081 attaatattt gagatacaag gctttcaatg gtgttgattt tatttaaatt gtgctgaacg
    13141 aattgtccga taatagaagg aaattgatca acaaaaataa cagctaatat caaatctaat
    13201 cctaatgtgc ctaatctagc gatgacaaat gtttgtaagc gttgaaactg tccaatttta
    13261 gtttgtttaa aaacaaatcg atcatttgta aaaaaagcaa aaagtatagc tattatgtta
    13321 gcaactattg cagagaatgg agcgtcctgc gagaaattaa aaatcccttg tcgaataact
    13381 aaataaagaa gggatgttaa tagtccgaac agtaaatata agctgacttc attttgaata
    13441 ctttttttca gtaacgattt taatttaacc atatttttat tatatcacgg atgcttcaaa
    13501 gtgatgagga aatctcccat aagcttggct ttactttttg tttgtgatat acttagtaag
    13561 ttgttttaca caacccttga tgcttagttc cctttcatca agcatattac aaacgttaga
    13621 agtgttaact gtctaaacgt taaaggagaa attatgaaca catttacaac tcgcgattac
    13681 gctcacatgg ctattgtaac agctatctat atcgttttaa ccataacgcc accttttaat
    13741 gctattgctt atggtgctta tcaattccgt gtttcagaaa tgctaaattt cttggccttt
    13801 tatcaccgaa aatacctttt tgcggtaacg ttaggatgta tgatttctaa tctttatagt
    13861 tttggaatga ttgatgtctt tgtaggtggt ggttcaactt tactttttgt ttatcttgga
    13921 accattttat tcaagcaata tcaaaaagat tatctattta atggccttat taacaaagca
    13981 ttctttttct tttctttctt ctttgctgct tcaatgatta cggtagcagt tgaattgaaa
    14041 attgtagcag gattaccttt attattaaca tggttaacga cagctgttgg tgaattgact
    14101 tcacttttgg ttggagcagt ccttgtggat aagttgagta gacacgtcga ttttacaaaa
    14161 taaatatatt ataaagcgcg agacattgat ctcggctttt ttgtttggtt ttatagttag
    14221 catagctaga aaatgctgca acttttatga gaaatgctcc aatttttatt aatatcctta
    14281 gtctttgtga taaaatagaa atatggaaaa tcgtatgaat gaattagtat ctttgttgaa
    14341 tcagtacgca aaagagtatt atacacaaga taatcccaca gtttctgaca gtcaatatga
    14401 ccagttatat cgagagttag tagaattaga agagcaacat ccagaaaaca ttttgccaaa
    14461 tagtcccaca catcgagtgg gtggtttagt tttagaaggc tttgaaaaat accaacatga
    14521 atatcctttg tattcattgc aggatgcttt ctcaaaagaa gaattgatag cctttgataa
    14581 acgtgttaaa gcagagtttc caactgcttc ttatatggca gagctgaaaa ttgatggtct
    14641 atctgtctcg ttgacttatg taaatggtat tttacaggtt ggggcaacac gtggtgatgg
    14701 caatattgga gaaaatatta ctgaaaattt aaagcgtgtt catgatattc ctctacattt
    14761 agaccaaagt ttagatatta ctgttcgcgg agagtgctac cttcccaaag aatcttttga
    14821 agctattaac atagaaaaac gtgcaaatgg tgagcaggaa tttgctaatc ctaggaatgc
    14881 agcagcagga accttgcgcc aattaaacac agggattgtt gcgaaacgta aattagcaac
    14941 ttttctttat caagaagcta gccctacgca aaaagaaaca caagatgatg ttttaaaaga
    15001 gctagaaagc tatggctttt cagtcaatca tcatcgttta attagctctt ctatggagaa
    15061 aatttgggat tttatccaaa ctatagaaaa agatagaatt tccctccctt atgatatcga
    15121 tggtattgtt attaaggtta atagcttagc aatgcaagaa gagttgggct tcactgtaaa
    15181 ggcacctcgt tgggcgattg catataaatt ccctgctgaa gaaaaggaag cagaaatatt
    15241 atctgttgat tggacagttg ggcgaacagg agttgtaaca ccaacagcta acctaactcc
    15301 agttcaacta gcagggacaa ccgttagtag ggccactctg cataatgtag attatattgc
    15361 cgaaaaagat atccgtattg gagatactgt tgttgtatac aaagcagggg atatcatccc
    15421 tgcagtctta aatgtcgtta tgagtaagcg aaaccagcaa gaagtcatgc ttattcctaa
    15481 actatgtcct tcttgtggta gtgaattagt tcattttgaa gatgaggtgg ctttacgttg
    15541 tattaatccg ctgtgcccca accaaatcaa agaacgttta gctcattttg ctagccggga
    15601 tgctatgaat attacaggtt ttggtccatc cttagtcgaa aaattatttg atgctcattt
    15661 aatagcagat gttgcagata tttatcgttt aagtatagag gatttattaa ctttagatgg
    15721 tattaaagag aaatctgcaa ctaagattta tcatgcaatc caatcttcaa aagaaaattc
    15781 tgctgaaaaa cttctttttg gcctaggtat tcgtcatgtg ggaagcaagg cgagccgcct
    15841 tttattggag gaatttggta atttgaggca actaagtcaa gcaagtcaag aaagtattgc
    15901 ctcaattgat ggactaggag gggttattgc caaatcactt catacgttct ttgaaaaaga
    15961 agaggtagat aaactactag aggaattaac ttcctataac gttaatttta attatctagg
    16021 aaaacgagtt agtacagatg cacaactttc aggtttaaca gtcgttttaa ccggaaaact
    16081 tgaaaagatg actcgtaatg aagcaaaaga gaaattacaa aaccttggtg ctaaggtgac
    16141 aggtagcgtt tctaaaaaga ctgatttaat tgttgcagga agcgatgcag gaagtaaact
    16201 aaccaaggct caagatttag ggattacaat acaagatgaa gactggttac tcaaccttta
    16261 agtgaggaag aaatgaagaa acaaaaaaaa gcgcgtttaa tttataaccc aacttctggt
    16321 caagaaatta tgaaaaaaaa tgtagcggaa gttcttgata tacttgaagg atttgggtat
    16381 gaaacatcag cctttcaaac aacgccaact aaaaattctg ccagagatga agctgctaga
    16441 gctgctcaag ctggttttga tttgattgtt gcagcaggtg gagatggtac tattaatgaa
    16501 gttgttaatg gtattgctcc tttgaaacgt cgtcctaaga tggcaattat tccaactgga
    16561 acaactaatg attttgctcg tgccttaaaa ataccgcgag gtaatcctat tgaagcaact
    16621 aaattaattg gtaaaaatca aatcgttaag atggacattg gtcaagctca agaggacaat
    16681 tattttatta atattgcagc tgcaggtagt ttgacagagt taacttatag tgtaccaagt
    16741 caattgaaaa caacgtttgg ctatcttgct tatctagcaa aaggtgttga attattacct
    16801 cgagttcgaa aagtaccagt caaaattact cacgataagg gtgaatttat cggtgatgct
    16861 tcaatgattt ttgttgccat cacaaattct gtaggagggt ttgaacaaat tgcacctgat
    16921 gccaaattgg atgatgggaa atttaccctt attttagtaa aaactgcaaa tcttattgag
    16981 attatgcatc tcattcgttt ggtgctagca ggtggaaaac atattaatga taaacgagta
    17041 gagtatatta aaacgtctta tctaactatc gaacccctat cagacgagcg tatgatgatt
    17101 aacttagatg gagaatatgg aggagatgct cccattactc tagcaaacct taaaaatcat
    17161 attaggttct ttgctaatac tgatgaaatt tcggatgatg ctttagtttt agataaagat
    17221 gaattagcga tagaagctat tgcacagaag tttgctaatg aggtaaatga tttagaagga
    17281 ttaggagatt gatgaatgga taatactgtc attgtacatt accatagccg aaagaaaaat
    17341 tactttaatt taagtttatg gcagtggcgc gatggctcag agggtcaaga tgctcatttt
    17401 tcacggtttg acagtttcgg tgctgttgcg atactaaaat atcccttgcc atatttttta
    17461 agccattatt atgtgattgt taaggataaa aattggcatc ataaaacaat agattatcgc
    17521 attgattgta ctaaaggtgg tgcacgtaaa gaagtttgga ttgtagatgg ggatgacaat
    17581 ctgtattatt ctcgtcaagc agcagttagt agccatcatt atagtagacg ccaacctcat
    17641 gcctacgata tggcaatcaa tagtagagcg tttgataaaa aatggggatt tgatggttgg
    17701 ttaggttttg aatattcaga aactaaaacg gcttttagat tgtgggcccc tactgcggaa
    17761 cgtgtagaac tgattttata tcattcgaca gatgaaacag caagtgtatc aaaagttctt
    17821 tctatgaaaa gagggacagc cgtcaattat aaaaatcata aagaaaacac ccatggcgtc
    17881 tggtttactg aacttgaagg aaactataat taccaagcat atacttatcg agtctactat
    17941 agacgacgta cctttaaaat tacgagagac ccttattcca ttgcaacgac tgcaaatggt
    18001 aaaaggtcta ttgttattgc tccagaagca ttaactccgg aagggtttaa aataagtcat
    18061 ggaaaagaag ctaagtggcg gctcgaaaac cccaatcaag cagtcattta cgaaatgcat
    18121 gtgcgtgatt tttcgatatc tgagacttca ggagttaaga cagattatca aggaaaattt
    18181 aagggattgc atcaaaaaga tacagttaat cagcatggtg ataaaacgac ctttgattat
    18241 gtgcaagact taggagttaa ctatatccaa cttcaaccaa ttttcgatca tcatcagacg
    18301 tttgatgatg atggtcacta tgcttataat tggggatatg atcctgaaaa ctacaacgtt
    18361 ccagaagcaa gcttctctag taatccacat gaacctgcaa cgcgtatttt agaattaaaa
    18421 tctgctatcc aagcgtacca tgatgcaggc attggtgtta ttatggatgt tgtctataat
    18481 catacttttt catcaactga ttctgcattt caactaacgg ttccagatta ttattatcga
    18541 atgaatcaca atggaacatt tcaaaatggt tcaggctgtg gtaatgaaac agctagtgaa
    18601 aaagaaatgt gtcgtaagta tattcttgat tcagttttgt attgggtgaa agaatataat
    18661 atcgatggtt ttcgatttga tttgatggga ctacatgatg tagaaacgat gaacattata
    18721 agaaatgagc taaataaaat cgaccctcgt attttggttt atggagaagg atgggacatg
    18781 ggagctggtc tcacgcctca aaataaagct aaaaaagata atgcttatca gatgcctgga
    18841 attggctttt tcaatgatga tgtaagagac gctgtcaaag gtgcggaaat ttacggagaa
    18901 tttaaaaaag gcttagtttc tggaaacagt acagaagata ttgttgcaaa aggcatttta
    18961 ggaagtgatg agttagtatc atacattgat cctagtcaag ttttaaatta tgttgaagca
    19021 catgacaact acaacttaaa tgatttgcta tgggaattac atcctaatga caacgagaaa
    19081 caacatattt accgagttga agtagctagt gctatgaatc tattgatgca agggatggct
    19141 ttcatgcaat taggtcagga gtttcttcga acaaagtgtt atcctacagg tgataaaggt
    19201 caacttactc aagcagataa agaaagagct atgaattctt acaatgcccc agaccaagtt
    19261 aatcaggtca actgggataa tgttaccttt cataagagta ctattaattt tattaggaaa
    19321 ataattactt tgaagacaaa ctctccttac ttttcttact ctagctttga agagattcgc
    19381 aaacatgttt ttgtagagtc tgctcaatac cattcaggtt tcataagttt tacagtcgaa
    19441 gaacataaga aaataaaaat aattttcaat attaaaggaa aacgcttgca ggatcctttc
    19501 ataggtgata taataatatc aaatc
//
LOCUS       DATRNX010000033        19344 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRNX010000033 DATRNX010000000
VERSION     DATRNX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19344
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1600)
                     /locus_tag="VBC81_000932"
     CDS             complement(<1..1600)
                     /locus_tag="VBC81_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6911982.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPISYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKL"
     gene            complement(1604..2338)
                     /locus_tag="VBC81_000933"
     CDS             complement(1604..2338)
                     /locus_tag="VBC81_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6911983.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            complement(2358..2663)
                     /locus_tag="VBC81_000934"
     CDS             complement(2358..2663)
                     /locus_tag="VBC81_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6911984.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            complement(2875..4860)
                     /gene="tkt"
                     /locus_tag="VBC81_000935"
     CDS             complement(2875..4860)
                     /gene="tkt"
                     /locus_tag="VBC81_000935"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6911985.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            complement(4985..6415)
                     /locus_tag="VBC81_000936"
     CDS             complement(4985..6415)
                     /locus_tag="VBC81_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6911986.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            complement(6406..7743)
                     /locus_tag="VBC81_000937"
     CDS             complement(6406..7743)
                     /locus_tag="VBC81_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6911987.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDTVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(7831..8529)
                     /locus_tag="VBC81_000938"
     CDS             complement(7831..8529)
                     /locus_tag="VBC81_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6911988.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            complement(8541..10370)
                     /gene="glpO"
                     /locus_tag="VBC81_000939"
     CDS             complement(8541..10370)
                     /gene="glpO"
                     /locus_tag="VBC81_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6911989.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(10383..11891)
                     /gene="glpK"
                     /locus_tag="VBC81_000940"
     CDS             complement(10383..11891)
                     /gene="glpK"
                     /locus_tag="VBC81_000940"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6911990.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(12006..12269)
                     /locus_tag="VBC81_000941"
     CDS             complement(12006..12269)
                     /locus_tag="VBC81_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6911991.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(12358..12615)
                     /locus_tag="VBC81_000942"
     CDS             complement(12358..12615)
                     /locus_tag="VBC81_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6911992.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(12627..14666)
                     /locus_tag="VBC81_000943"
     CDS             complement(12627..14666)
                     /locus_tag="VBC81_000943"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6911993.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDIHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(14670..15311)
                     /locus_tag="VBC81_000944"
     CDS             complement(14670..15311)
                     /locus_tag="VBC81_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6911994.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(15311..16225)
                     /gene="glyQ"
                     /locus_tag="VBC81_000945"
     CDS             complement(15311..16225)
                     /gene="glyQ"
                     /locus_tag="VBC81_000945"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6911995.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            complement(16538..17080)
                     /locus_tag="VBC81_000946"
     CDS             complement(16538..17080)
                     /locus_tag="VBC81_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6911996.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            complement(17198..18346)
                     /gene="nagA"
                     /locus_tag="VBC81_000947"
     CDS             complement(17198..18346)
                     /gene="nagA"
                     /locus_tag="VBC81_000947"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6911997.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(18624..19331)
                     /locus_tag="VBC81_000948"
     CDS             complement(18624..19331)
                     /locus_tag="VBC81_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6911998.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
BASE COUNT         6204 a         4003 c         2999 g         6138 t
ORIGIN      
        1 caagcttctt ccaaaaattt tttagcagta agtattggca aattgataat atgagcaacc
       61 caatgataag gattatgata ttgacagcta cagtattcca taccattgac caaaaaatac
      121 ttattccaat aagaaaacta cctattatca tcattccaaa caatgaacca actaacagcc
      181 attggcctaa ccctctatta aatagttgcg taacctcttg ccaattcaag aacaaatgtt
      241 tatagtcacg tctataaata aactggcctt cgataagtcc catgaagact agacctaaaa
      301 tgaagctcaa taaaacgagg attgatagtt taagtagaaa gaatcctacc aagaaataaa
      361 taaaagtagg aactgcaagt tgtaatgtta tcaaagttac aaatttatgc tttaagaaat
      421 acgaaaatga tataggcaaa ctttttataa agtagaaatt acttttttct aaagaaatac
      481 ctacccctac aatgctagct ggcatcacac ataaactacc tattaatatt cctgctagaa
      541 aagctatccc aaaataattt tcattaaaaa atcgaccaat ctctttacca ttattcaaaa
      601 ttggaacaat gaacattgct agatataata gtggcattaa aaatgtattt gtcaataatg
      661 tcgcattttg taaacttgat aaatggtgtt taacaagagt tttattaatt gactcacgtt
      721 tactaaaatc gatttgacga tggacttttc tctgcttgac ctgtccatac aacaactcct
      781 gatagtagcg tggcataacc catttataaa tagcacctat caagaataaa atgattaaga
      841 gcgggagata atagttgaga agactctcca tactaaacgg cgctgtaaca atatcataat
      901 aacctctgac ataagaaatt ggagcgatat cttgtaattt cccttgtacc atcttttgac
      961 tggtcatatt cacaaataat aagggaacta tcgctccaaa tgtagctagt gaaattaata
     1021 tagtagaaat aagtttccga ttagcacttc gaataatggc acggcctatt aaactattaa
     1081 tatacaatga aatcaccgtt accgataaaa ataaaatagc aaaatttatg attgctagga
     1141 ggaccgcaag tgggccacct attatacgcc aataagccac aatcattaag gttaggattg
     1201 gcattagaaa agttacagac atcccaaatg ttgcaaaaat tttagcaaca taaagctctt
     1261 cagatttaat aggcaaataa gcatattgtt taacatcgtc actctcatag aaaacactgt
     1321 acatcgcaat aaaagagtag ataatggata ctagtgtaaa gataccaatg tagaacgtga
     1381 aatagcccgg atattcttta aaatcaagtg atacaaagag aaatacataa attattgaaa
     1441 agagcaaaat ctgaaataac tgatttctaa acatggattt ataagctgaa aattcttttt
     1501 taggatgctt ttcttgcttt tttcttagtg ccgatagagt ctgagggttt gaataaagga
     1561 tattaatttt tacgagttcc cagatacgtg accaattcat actttatccc tccctattag
     1621 cttgacgtcc tgctaattcc aaatagatag tttccaaatc tttatctgga tattgcattt
     1681 ttaactcacc taagctaccg acaaagatta atttcccttg tttcaaaata cctattctgt
     1741 cgcataactg ttctgcaact gctaagacat gtgttgaaaa aataactgtt tttccatttt
     1801 tagcatgttc tttcatcatt tctttcaaat caaaagaagc ttgaggatct aaaccagtta
     1861 atggctcatc tagaatccaa atgtcaggat ttggaagaag ggcacctata acaatgactt
     1921 tttgacgcat accatgtgaa aaactttcaa taggattata gcgctgctcc tctagttcaa
     1981 atatgtctac caattttgtg atacgtgctt caatatcttc tggggcaaca tcataaattt
     2041 tagctaaaaa gtaccagtat tcctctgctg ttaaattcaa gaaaatatct ggcgagtccg
     2101 gtacataacc tatttttttc ttaatttggt cacgatgttc tgtcaaaagt aggtcatcaa
     2161 tatagacttg gccataagtt gcatcaataa tagatgttag aatactaatt gtcgttgttt
     2221 ttcctgctcc attgtgaccg attaatccaa aaatctcacc atctttaatg gagagggtta
     2281 aatcactgag agcttctttt tccccgtata ctttcgaaac atgttcaaac ttaatcataa
     2341 aaatcttctt ttatctccta atgatttcca aatactttag aaagcaatcc cgttgttacc
     2401 ttattttgct catatctctc agacaatttt ttagcagatt tttttaattc cttaactgta
     2461 gctaatgact cgaaagattt ttctatctca ttattttcca attgctgagt taactcataa
     2521 gcaacacgag gtacatcgct agtttttatc aaaccattcg atgctgataa tagtattgat
     2581 gatagcactg catcttcacg gacagatata tcattgtaag tatgctgtat ctgccgataa
     2641 aaatcacgac actccttctt cataaacaaa cctttctaaa aagataatta ataatagttg
     2701 tattatttta tatatacaat agttttatca attatactgc gctatggaat gtttgtcaaa
     2761 cgttttcaaa aaagagtgct ttccatcact tatcagtcta tcaaaactat tctcaaatcc
     2821 atcctatatc ttacaaaaag aagttgaggt atttttccca acttcttcat gctcttaaag
     2881 ttctttatat agcgatacag cattctcaac tgtaaagcca tactcttcga aaattctgtt
     2941 acctggtgct gatgctcccc aagtatcaat agtaagtgtt agcccattta aaccaacata
     3001 tttaccccaa ccgaagcttg atcctgcctc aatagcaaga cgttttgtaa cagctgatgg
     3061 aagcacttgc tcttgatatg aagcctcttg ttcatcaaaa atgttttgtg ctggcataga
     3121 tacgacgcga acatggatgc cctctgactc aagtttgtct tgagtatcta aagctaattt
     3181 aacttctgaa cctgttgcaa tgatgatacc atcaagttca cctttagctt ctgataaaat
     3241 ataagcacct ttattaaccc cttcttgggc taattctgat gttccttcaa gaactggtaa
     3301 gttttgacgt gttaaaacaa gcatcgttgg tcgatctgtc tctgatacag ctcgttgcca
     3361 tgcagcgttt gtttcattac cgtctgcggg acggataacg ttcaaattag gcattgagcg
     3421 cactgatgct aattgttcta ttggctcatg agttggccca tcttctccaa cagcaataga
     3481 atcatgtgtc ataacataga cagttggcaa gttttgaagt gcagccatac gtactgctgg
     3541 taacaagtag tttgagaaga caaagaatgt acccccataa acacgagtcc caccatgaag
     3601 cgcaattcca ttcattgcag cagccattgc aaattcacgt acaccaaacc aaatgttacg
     3661 cccagcgtag ttacttgctt ggaaatctgt ttctgctgct accattgtgt tattagaagc
     3721 tgagaggtct gctgaccctc cccagaggtt cggtaattga gtcgataatt gttgaatagc
     3781 ttgttggctt gaaacacgac tagccactga tgaacctagg tcatgagctt tcaattcaac
     3841 tgtctcagct tggccagcaa aagcttcttt gtattcagct gctaattctg gatactcaac
     3901 ttcatattta gcaaaaagat cattccatgc ctcttctgcc ttcgcaccac gcgcttgaag
     3961 atctgaggca aatcgagctg caacttcagc aggaacagta aagtctggat attcccatcc
     4021 ataagccttc ttggcaaaag taataccttc tgcaccaagt ggcgctccat gaactgaaga
     4081 tgttccctgt ttctctgcgc caaatccaat aatagtttta acttcaataa tggttggttt
     4141 atcagtttca gctttagcag cttcaattgc tgcagcaatc gcttctaaat cattaccatc
     4201 tttaactaag atatgttgcc aaccatatga ttcaaaacgc cctttaacat cttctgtaaa
     4261 tgattgagac gttggaccat caagtgagat atcatttgaa tcataaagaa ggaccaattt
     4321 accaagtttt aagtgtccag caagactggc agcttcttgg ctaacaccct ccatcaaaca
     4381 accatcacca tgaagtgtat aagtgtaatg atcaactaaa tcaaagcctg gtttattaaa
     4441 tttagcagcc aaatgagctt cagccattgc cataccaaca gcattagcaa taccttgccc
     4501 taaaggtcca gttgttgctt ctacaccatc agtatggtta acttctggat gtccaggtgt
     4561 ttttgatccc cattggcgga attgttttaa atcatcaatt gataaatcat agccagctaa
     4621 atgaagtagg ctataaagaa gagctgaacc atgcccagct gaaagtacaa aacggtcacg
     4681 gtttgtccaa ttgcgacttg tttttgggtt tacatttaag aatttattcc aaagcacata
     4741 agccataggc gcagctccca taggaagacc tgggtgccca gaatttgctg cttggatagc
     4801 atcaatagaa agcgtgcgga ctgtattaac cgctaattga tcaatggtgt caaatgtcat
     4861 gataaaacct ttctgttttt aacaactaaa tttaaaattg ttcgtctttg atattataac
     4921 ataaaatacg taaacgtttt aacaatttct ggaggaatta aaaccattca attaagtaat
     4981 gggattaatt gtcataaata agtgaatcta tttcctgtag ttctcgctct gaagcacctt
     5041 tattaaggca ataggttagt ttggctttat aaggtcttga gcgactctga taaataacca
     5101 tatcaaatcc ctttccttcg tcataaggtt cgacttgaat agaattatta ccagagaaat
     5161 gttgtcgaat gcgatattcc aacaatgact gttccacagc attacctgtc atatctaggc
     5221 caatcctaaa gggcattttt tgactatttt tcgagaaaat aagcaattcc aacaaatgta
     5281 aagtcagcaa agaacaacca tcttctgtta aaggtctaat tcgattaaca tttgcaataa
     5341 tcttctttga aatatcatgc aacttatccc tcatattatg atcaataagt cgatacgttt
     5401 gttctaaatc aggttgttca actaaaatat gacctttaaa gaaataataa tggctatata
     5461 actgaccaag ttggtaaata acctgatctg atgtctcgtc agctaaaata ctctcctttt
     5521 taaagtagat gattgtttcg gttacacaat caataatcgg gcctccaaat cccatgatgt
     5581 attccattgt attgattggt aaaatatttt gtgaatgtag aaaagcaaaa gttgccagaa
     5641 cttctccttc cctatactca agagcaaaac gactcatata caaaactaag gctgcgtcta
     5701 accgtttata aaaagcatta cattgataat gttccacaat gggcttgaga ttatcagata
     5761 gtttttccat agcaaggaga cgatgatgac aaatactaaa ccaaagcccc agattttgct
     5821 gagcttctcg agttaaagta tgttgagcca agcgctctat taatagaatt aatgagtcta
     5881 agtctaattt ctttatcata ttttggcact tgtcataaga ccatagttgc ttgaataatt
     5941 cataataaaa ataacgccat tgcaattcat caccaatctg ttttccttgt gaaatagcaa
     6001 tatcaaattc cgctaacaat tcattgagat gagcaagatg acgatttaaa gtagcctcac
     6061 taaccatcaa ttcttgagaa agaccaacga ttgtgaactg acgctgatta aataaagata
     6121 ttaagatagt gtatttaata ctatctttca tcagagcttt caacacttct tgattggata
     6181 agttataatc ctcttctaaa aataacttat ccttataata aacgattcga caattaactt
     6241 tttcttgttc aaagtagcta ttaagattat tgatatattt aagcaaagtt gctcgactta
     6301 aaccagtttc taataaagct aacttcagtg ctattgcctg aggagagcct cttaaaaaag
     6361 aaagtagccg atattgaaca cgcctttctt tttccattag agcttctatt cgcatagccc
     6421 aatcctcttt tcataaaggt tgatagctgc ttgatgtaac atataagcca tctcagattc
     6481 tctagctgta taaatgaaat cttgaaaagc caaatcaaaa tctgtcatgt cacacgatat
     6541 agcttgaaca agctgattgg cagtagccaa acaattttcc tttgagatta attgagcccc
     6601 caataaatgg cccgtttttt gattagcaat caattttatc cttaaatcat actgttcttt
     6661 agggaaataa gtaacactaa cggtatcctc gtaaagacca gcttccagct cagttaatcc
     6721 aatgttagct ctataatagc caaagtgttt agatcctgta gcgcgtgtta tttttaattt
     6781 tggaacagca tgtcccgata aatggtatgc caacatttgt gccgaacgga ttgcttggtt
     6841 aattaagggc atataataag cactattaag acctttaaaa tacgaccaaa tcaaatcgcc
     6901 aatagcatag atattagggt cactcgtttg gaaataatca tttacaacaa cactattatc
     6961 aacacttaaa tcaaccaacc cagttaaaag acgactatcc ggacgaaaat taacagctaa
     7021 tatgacagta tcaactgtaa aggtgtctcc tgttagcgtt cgcaaacaaa gtttttcttc
     7081 tcgggttaca gtaattttct caaccttttg attttggaaa aaatgtagtt ttgattcagc
     7141 catcgcatca agtaaaggaa gggacatatc aggatctgta tgcctgaagt ctggtctttc
     7201 ttgagcttct aacaaagtga ccgccttccc tgattcatgt aaactataag caatatctaa
     7261 accgatgata cctgccccaa caactgctac cttatgactt ttatctaata cctgcttagc
     7321 attttgacta gttgcataag tttttgttgt taaaacacct tgcgcatcag ccccatcaat
     7381 ataagttgat tctgcttggg agcccatggc acaaatcaat tgatcataat agacagaaga
     7441 accatctgat aacattaatt ccctatcttc tttattaatt ttctcaactt ttagaccaaa
     7501 tagacagcgt atattctgca accgttgctc ttcttctgtc tgccacatgg cctgatttaa
     7561 accactaatt tcgtgacgat aataccaatt taagccattt ggaacataat ctggatgaat
     7621 ttctttatca atcaaaacga tatcccaatt aggatttaat tggcgtaatt tctgcgcaca
     7681 agtcatacca gcaaaagaag ctcctaaaat gacaattact ttatttacat atttttctgt
     7741 cataattact tattataaca aaagaagtta tttttgaaaa cggtaacaaa aaactgagac
     7801 cataaagtcg gtctcagcat caaaaatctc ttacatcata gcaaatatta aagctgctaa
     7861 aatagctcct accataggtc ctactatcgg aatccatgaa taagtccaat cagaatcacc
     7921 tttgttcttc atcggtaaca aggcatgcaa taaacgagga ccaaaatcac gtgcaggatt
     7981 aatagcataa cctgttgttc caccgagcga tagaccaatc ccaaccacta acataccaac
     8041 actcatcgta ccaactcctg gaggcatatt gtattttccg atggcaaaaa tagtaagtac
     8101 aagaacaagt gttcccaata cttcacttaa aaaattggaa aaattatcct tcaaagccgg
     8161 accagttgca aaagttccaa ggatatctgc tttattttct gcagcctcat aatggggtct
     8221 aaattgcaaa taaacaagaa gtgaacctag cattgcacca ataatctgtg caatcatata
     8281 aagaatagct gttccccaag ctaaatcacc tttgatcgca aaagctaatg acacagctgg
     8341 attcaagtgg gcagggctaa tattgccaga taccaacgct gcaatagcta cagcaagccc
     8401 ccatccaaat gtgataacaa tccatccaga attatgattc ttcgttttag gcaaaacaac
     8461 acctgctaca acaccattac caagtaagac taagagagct gttcctaaga attctccaaa
     8521 aatatccatt attatctcct tcaattatcc ttcaaagctg ccaagtcatt gtcttttaga
     8581 gtctcatgta attcttcttc atagaaagtt ttgtcttgat cagaccattg ataatgttta
     8641 gccatctcat caataacagg ctgaatcaaa gaatctaaat tatctctcat gaaaagcata
     8701 tgattcgtac gtcttaagaa gaaatccgtt gggctaagcg ccatttcata atccatagca
     8761 tagtgaagtg acaacgtttc taccaagctc agaccttctg cttctgtaat ttggcgtgtc
     8821 aatgcaaata attttggtgc atttgaccca taaaggttag caatataacg tgcatcctca
     8881 atacttaaac cacttagtgt gcccaattga gcataagctt caatttcaga atcaacatta
     8941 gatgggttaa tctctcctcc tgaaacagga taggttttag aattgattag tttaaacttg
     9001 cgattatatt ctttatccaa aatgttaata atagtttcca tggcaccttc tgccattttt
     9061 cgataatcag taattttacc accagcaagt gttaataggc cattatcatc acgctcaaaa
     9121 cttgatcctc gagaaacagc tgatgggtcc aattcttttt cagaagtact agattctaca
     9181 tgagaaatag ctttttcaac atcctcacga tgatttttat gtgcaatata atctttaacg
     9241 gaatcaatta attcttcaaa actttcatcg cttagttttc cactattgcc tccattatag
     9301 tcagaggcac tattccctga tagtagtgga cgcagacctg cccatgaact ctcaatatca
     9361 tcaatagtaa gctctgcttc tgggaagcgt ttgtttacaa tattaaggag gtagtcaaca
     9421 tcttctttag ttaccgtagg atgttctaag tctccatggt aatcagtatc tgtggttcca
     9481 aaataagtct tatcttcacg tggcaaaaca aaaatcatgc gaccatcatt tagacctgta
     9541 tcaacataaa caggttgaga aacatttaat ttttggcgat ctacaactaa gtgaactccc
     9601 ttagttggac gtaattgatg aatttgttta ccttcattgg agaagttgcg aacggtgtca
     9661 ctccaaggtc cagttgtatt aataacaagt ctagctttta tatcgataac ttggtcagtc
     9721 aacaaatcac gcgcacgtac tcctataatt tgattgttgt catcaaataa gaagtcctca
     9781 gctttgacat gactggcaat ataagctcca tccctattag cacgtttaat attttcaatc
     9841 actaggcgtg catcgttatt acggaaatct aaatagaccc ctccgcctag caaaccttct
     9901 ttttgtaaat caggctcacg cttaagaaca tcttctgcgc ttaaaacttt attggctgcg
     9961 ggtgtgtttg tcacacctgc taataaatca tataaatcca tagccacttt aaggcgaaac
    10021 atactaaaag tagaacccgg ctcatcgtaa accggtaaaa gcattggatc tggcttagga
    10081 atgtgtggtg caatttgctg cacgactgca cgctctgata ctgtatcaga aacaacttct
    10141 acatcaaatt gcttcaagta acgaagacca ccatggacca attttgttga gcgactactt
    10201 gtcccttctg caaaatcttg catttcaata agtccagtat ctaatccact agctgctgct
    10261 tgtaaagcga caccagcgcc tgtgataccc ccacctatga taagtaaatc cagagtacga
    10321 tcttgcattc tttgtaaggc taaacgtcgt gtttctcgtg aaaattccat ctctacaccc
    10381 ccttaatcct cttgagcaaa tacttgtgtc gctttgacag cttttctcca ccctttatac
    10441 agtttttctt ttcgagactc attcattgtt gcttggaaaa gttgtcctgt tgcgttcagt
    10501 tcttttagtt catccatact ttcccaatag ccaactgaaa gtccagctaa gaaagctgca
    10561 cccaaggcag tagtttcaag gttcttcgca cgagcaatat caatccctaa aatatccgct
    10621 tggaattgca ttaataaatt attcattgca gctccaccat ctacacgaag ttgttggata
    10681 tcaattcctg aatcaacttg catagtgtca ataacgtctc gtacttgata agcaattgac
    10741 tgaagtgtag ctttaacaaa atcttcttta cttgtcccac gagttaatcc aaatactgac
    10801 cctctagcat ttgaatccca atacggtgca cccaaacctg taaaagctgg aaccacataa
    10861 acctcatcgt cacttgttga tgattgagca aggccttctg actcacttga cgtttcaatc
    10921 attctaagac catcacgtaa ccattgaata gcgcttcctg caatgaagat tgatccttca
    10981 agtgcataat gaaccttacc attaatgccg taaccaatcg ttgttaatag attattttga
    11041 gacagttgca tctcttcgcc cgtattcatg ataatgaagg aacctgtacc ataagtgttt
    11101 tttaccatac caggttcaaa ggccaactgt ccaaataatg ctgcctgttg gtcaccagcc
    11161 ataccagaaa ttggcacctc tccaccataa aaatggaatg gagtagtttt cccataaact
    11221 tcagaattag attttacttc tggtaacatt gctttaggaa tattcagtaa ctctaaaatt
    11281 tcatcatccc atttaagttc tttaatatta taaagcatcg tacgagcagc atttgaatag
    11341 tctgtaacat ggacgagacc gtcagttaac ttccaaacta accaagtatc aattgttcca
    11401 aaaagcaact ctcctttttc agcacgttcc tgtgctccag gaacatggtc taaaatccaa
    11461 cgaacttttg ttgccgagaa gtaagcatca atgacaagac cagttttttc atgaatcata
    11521 ttagtatgac cttcttgctt caattgatca gcaatcgggg ctgtttgtcg cgattgccat
    11581 acaatagcat tataaattgg aagacctgtt tttttatccc aaacaacagt tgtttcacgt
    11641 tgattggtaa taccaatagc ctctatctgt ccaggtttga tgcttgattc aataaaagct
    11701 ccagcaatga cagattgaac agaattccaa atctgattag cattatgctc tacccagcct
    11761 gcttgaggaa aaatttgggg aaattctttt tggctactcg caattttttc acctttttta
    11821 ttaaaaataa ttgctcgaga acttgttgtt ccctggtcaa tcgccataac gtatttttct
    11881 tctgaagaca taaacttcct ccttaaaagt aagcgttttc tttttgtaat tctattatac
    11941 ctaaatcagt ctatcctgca ctatcaaaaa tttaatatgc ccctaaaatt tttgataaga
    12001 tatcatcaat aacaccaaat tagccaaata atagctaaat caataatcat tccgatatac
    12061 caaactagtc taaaatacca tttagtcgtt ttatgtcgaa aaacttctcc acctaggaat
    12121 gcaccaaatc cccctaataa taaactctct atcaacaatg ttttctcact aatacgccat
    12181 gcccctctaa ttgcacgcct tttatcaata gcataggttg caaatactaa aatattccat
    12241 aacagtaatg ctaatagtaa ttcaatcatt attttttcct tcctaataaa aaaggcgaga
    12301 caaagattat cttttgcccc acccaagtgt tgactttaat ttaaaattgt ctcaccatta
    12361 agaatttgga tcatccaaac tacgaccatg tagtcctttt tctctctgga cttgacgaag
    12421 tttttccggg gtaacatcat tgccctcatc atccactaat ttaattcctt caacgtggtg
    12481 acggactgat cggcggaaac cttcaatata ctcttcacgg agctttgctt gttcaacctt
    12541 ctcttctccg gtcaatccaa ctgttttttt cttttttgaa agctcgttaa tacgagcaat
    12601 ttttttagga tccataaaat gcctccttat tttgtgttta ataagttaaa agcagcgact
    12661 gttcttgcct ttgcaacaag gctattgagt aaagccagac gattttgttt cattttttca
    12721 tcatctgtca ttaccattgt attatcaaag aaatcattaa taattggact aagtgcgaaa
    12781 agcttatcta agttgtcatg aatatcctca gtcagttcaa gtgacaaaat agcttgatat
    12841 agcgccttct cctgattatt ttcaaacaat gaagagtcca ccgatacaga atgagtaact
    12901 ttttcagcta aattaaatgc acgtgacaaa ctttcaacgg aaactttata ttgagcatcc
    12961 ttgctctttt gcaccaatgc ttggctagct tctagaataa ggctaacaat ataagtgtct
    13021 gattctaaaa cggcttctcg aatatcttta gggaccttag agccaatcat cttttcaata
    13081 cgagctgaaa taaaggccat gacctctttt tgattagcat aatctaaact agcaaacgat
    13141 agaccataaa ggttagttac taattcatca agtgggatat cccaaccaaa agcttccaaa
    13201 atacggacaa tcccctgagt agctcgacga agagcatatg gatcatttga accacttgga
    13261 atcaaaccaa ctgagaagaa tgaaagtaag gtgtcaaatt tatcagctaa cgctagaatt
    13321 gcaccaattc tggtttctgg aagctctcca tcggccgatg tcggcatata atgttcacgt
    13381 atcgctgctg ctactgcggg ctgttcccct gcaagtaagg catatttctc acccataata
    13441 ccttgtaatt catcaaactc atcaaccatt ccagttaaga ggtcaaattt ataaatactt
    13501 gcagctcgaa gcacagctaa tttttcttca tcagaaagac ctgccttatc agccaaatgt
    13561 tgtgaaataa cttttacacg atccatatgt tcataaagtg aaccaatttt ttcatggaaa
    13621 gttacttgtt ttaatttctc aactaaatct gcaatattaa gtttctgatc ttcttgccag
    13681 aagaattcac catcttcaag acgagccact aaaacttttt cattgccttt tataacattt
    13741 tcgatatgct cagcatttcc attacgcaca gaaataaagt ttggtaacaa ttttccatcc
    13801 ctgtcgcgaa caacaaaata gcgttgatga tttttcattg aagtgactaa aacttcttca
    13861 ggtacatcaa ggtatttctc gtcaaatgaa cccaagaatg ctgtcggata ctcaactaaa
    13921 ttaagcactt catttagtaa atcttcgtca atttcaactg aaatattttt cttttcttcg
    13981 atagcatgaa tttgattaac aatcatttgc tgacgttctt ttgcatcagc aataacaaat
    14041 tgttgtcgca agtcatcttc atacgaactg gcagatgaaa tttcagtgtc agaacccaag
    14101 aaacggtgtc ctcgactaat acgccctgaa tgaatatcaa ggaagtctag ctctaaggct
    14161 tgatcatcta ataataccac taaagtgtga actggacgaa tatattcaaa actattattt
    14221 gcccaatgca tgttcactgg gaaagtcaat tctgttaata cctctgtcac actcgctaaa
    14281 acatcaatcg cactttttcc agtttcatgc ttagtgacat aaacatattc ttctccctta
    14341 acttcacgaa attcgatatc atcaacactt aaaccttttc cgcgaacaaa accttgggct
    14401 gctttagaaa aattcccttc tgcatccaaa gcaattttct tagaaggacc cttgaaatct
    14461 tctgttaaat ctgtctgctg gtctgccaat cctttaacac gaacagccaa acgacgtgga
    14521 gttgaaaaag tttgaacagt ttcaaatgat aaacgatgat cctctaaaaa cttgaccatt
    14581 ttttggccca attgcttctc actcggtgtc acaacataag ctggcaactc ttcaagtcca
    14641 agctccaata ataaatcttt agtcatttcc tactccttac tataaaacgc ttcaaatgct
    14701 tctcttaaat cctgattcat cttatcaaga acaacttgcc tatctaaaat atcggtacct
    14761 tctgcacgaa caacacagaa atcatcaaaa gccataaaat ctttaaagat agcttttaaa
    14821 taatgaacag gaaattcata tggactatat tgtcctttag aataaataga acccgcagat
    14881 tctaacataa gcagacgata gtcatctgtc attaatccga ccgaaccatt ttcagtgtac
    14941 ttgaaagtct cacctgcaat aaaaatatta tcaatataag tttttgctct agcagtcaca
    15001 ttgtaattgt gcattggaga aacaaagaca atacggtgat gtgacttaaa ctgttcaagt
    15061 aaatctttag agattttagc ttgaacaatc tcatcggctg ttaattccaa accctttctt
    15121 tgtttagacc aaatcgaaag tagtacttct ttcgtaattt ctggcaaaac acaatcatat
    15181 aaatttaaaa tagataactg atggttaggg aaatgctcat tataaagttc aataaacttt
    15241 tcttgtagaa tagttgtaaa tgagtacgga ttcgaaaaat caggatgact attaacaatg
    15301 agtgtgttca ttagtcttcc tcagcaagta atttgatacg agtttcctca tccaaaagtg
    15361 ggaaaccaag ttttttacgt tcggcaacaa aggttttagc aacaacacgt gctaaattcc
    15421 gaatacgtgc aatatagcct gcacgctcag taacagatac tgctccacgt gcatctaata
    15481 aattaaaggt gtgtgaacat ttaaggacgt agtcataagc tggatgaaca agtccttctt
    15541 ctaaagcacg ttttgcttct ctttcaaatt tctcaaagtt ttcaagtaac attacttggt
    15601 ctgatatctc aaatgaatat tttgaatgct catactctgg ctgagtaaag atttcaccat
    15661 atttaacacc tggtgcccac tcaatatcat aaacagaatc tacctcttga atataagagg
    15721 ctaaacgctc tagtccataa gtaacttcag aagttacagg acctgtttga agtccaccta
    15781 cttgttggaa ataagtaaat tgtgttattt ccattccatc aagccaaact tcccaaccta
    15841 atccagctga acctgtagat ggattttccc agttatcttc aacaaaacgg atatcatgct
    15901 caagtggatt gatacctagt aattccaaag acttcaaata aagttcttga atatttgacg
    15961 gcgaaggctt cattaccact tgaaattgat gatgttggta aagacggttt ggattttcac
    16021 catagcggcc atcagctgga cgacgtgatg gttcaacgta tgcagcattc caaggctcag
    16081 ggcctatagc acgtaagaaa gtataagggc tcattgtccc tgctcctttc tcattatcat
    16141 aagcttgcat taacatacag ccttggtcat tccaaaactg ttgtaaagtt agaataattt
    16201 cttgaaaagt taatttctta gacatcattt tccttctttc atactttatt acagctgttg
    16261 ggaaggtgac aacaattgac ttaaaacgga caaatgataa aaaccacgtc ctacaccacc
    16321 tatgcccaac agcacagggg cgtcaaagac gcggttccac cctaatttat tacttcattt
    16381 ttgattgtac ttttactcac taaaaagtgc caatattcat cctattctac ccaactttca
    16441 ctaccttgag ctcgctaaaa aaacagctga tgaacacctc ttcttactga gacagtataa
    16501 caaaaaatga cttttatgtc tactatttat cttattctta agatacccta gactttttga
    16561 ttccatttgg aataatgatc gtttttaata ataagttttc ttcacgaatt cttaaataaa
    16621 taacaaaagc ataaattggc aagacaaata atgctgtata cttagcatga cacaataaag
    16681 ttacacctaa aagttcagga gcaatattta agaaatagtt aggatgctta acaaccctaa
    16741 atagccagtg atctacaaat tgatgatttt tatctaccat taatttcact gtccaaatac
    16801 gaccaagtaa acgtgttact gtatgtaaca taaacacaga aaaaatcatc aagctaagcc
    16861 cgattacgct aacaaaatta aaacttgctt ttgttagaac agcctcagtt acagatgaaa
    16921 aataaatgat aatatgaaga actgtgattg cttttgaaac acctacacca tattctttag
    16981 caccattttt tcttaaggcc ttttcattag ctatagatag ctttaaataa gctaaacgaa
    17041 tgataaacat agcggccatt aacccaataa taattgacat tgtaaactcc tattgttttg
    17101 tattatatta caacattata agaaaaatag ttttattgtc aaactatatt tcattaaaaa
    17161 ttaataaatt aaaaagaact agtaaaaact agttctttca agattgccaa cgtttttctc
    17221 catcaaggta tgttgcaact aaatctagat gtttatctaa tacaataaaa tccgctgcat
    17281 agccttcttt tatctgtcca cagacattat caatgcctac agacactgct ggaacataac
    17341 tcgccatacg cactgcctct tccggagtag caattcccca ttctacaaca ttttttaatc
    17401 catctttgag cttaagaata gaaccagcta aattccctcc atctttcaaa cgcgcagttc
    17461 cattagacac aaccacgggc aattccccta acatataatc tccatcaggg gcacctccag
    17521 ctgccataca atctgttatt aatgccacat gattatggcc tttttgtgtc attaaaatgt
    17581 cacaagcaac aggatcaacg tgatgaccat cgcaaatgag ttcagcataa gtattaggta
    17641 aattataaac cgcaccaacc atacctggtt ctcggtgtgt caaaccacgc ataccattat
    17701 acgcatgtac ccaaactgag gcacccgcct tcactgcctt tttagcttct ttatatgtcc
    17761 cattagaatg acctaaagct accgtaacac cttgctttgt gattgctgag acaaattcct
    17821 caactccctc acgttctggt gccaaagcaa tttttgtaat caagcctttt gcagccttct
    17881 gccattgagc aaactcttcc aagtttggat ttctcatata aatcggattt tgagctcctt
    17941 tatattcctc agtaaaatat ggtccctcaa aatagatgcc ttgaattttt gctccttgtt
    18001 ctttgcccgc tactgccgca actgctttag acgcctcttc tagtcgcttt gtatctgaag
    18061 ttagggttgt cggcaagaaa gatgtgactc cagtagatag taagccagca ctcattcgca
    18121 aaattccttc tgaatcacaa tccataacat cagcgccagc aaaaccatga atatgtgtat
    18181 caactaaacc aggtgctatt tggtaacctg aataatctaa aatctcctct tgtccagaaa
    18241 tattttctat ccatttacca aagtgattat cttttatttc taaataaccg ttttccttta
    18301 catgatcagc ataaaagaat cggtcagcct tgatgtactt cgtcatcttc ttccccccat
    18361 tcactctcat tataacgctt taaggtattt ttgtaaatgg tatataccta ttttttaaaa
    18421 agtcatggtt tattctaaga aagaactata cacttgttat aaaccacttc acttggttgt
    18481 acttttaagg ttttaattgc tgttgctcat aattcttttc cttctccaaa actatcaatt
    18541 gctaattctc ttatagtttt tgatgagaat ggctaattaa aaagcatagc aaaaaagtct
    18601 agtaaaacta gactttcagg attttactga gcaacacccg tcccattagt agcaggtaca
    18661 gtaccagttc ctggagtcgg aacagtattg ttaacccctg gagtaactgg tgtattattc
    18721 gtttgcggtt gctcataagt tggtgctaca tcctgattaa cgtaactaga agtatcctga
    18781 ctagcaggat tagctgtccc ttgttctgta gtttcagcta ctgaactaga gggagtttga
    18841 ttcccaatca aaccatattg tgtcttaaca gtatcaatac gtttttgtaa agcttcttta
    18901 gattctttga cagttaactt tttgactgct gcttcagctt ttaacacatt ttcttctgaa
    18961 ggggcttgtt ctagaatttc tactaaatct gtagcatctt tggtagcttt cttatctgct
    19021 tcgtctttaa gatacttatc ttttgctttc ttaaaacgtt tttgcaaagc ttctttttta
    19081 gaagaattgg acaatttatt taaagcaagt tcgacatcct tcaataattt ttctgaaggt
    19141 ttcttctcaa atttatctaa catatcttgt gcctcagtta attgagaatc actgacatgg
    19201 ttaacactgc tttctacagt atccatttga gtagtgccat gatctttaaa taagaaaaac
    19261 gtaacagaag caaaaattaa taaaataata ctagcaataa gtgctaaaag ttctttttta
    19321 tctattttca tactcactcc taaa
//
LOCUS       DATRNX010000034        18698 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRNX010000034 DATRNX010000000
VERSION     DATRNX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18698
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            287..1636
                     /gene="gorA"
                     /locus_tag="VBC81_000949"
     CDS             287..1636
                     /gene="gorA"
                     /locus_tag="VBC81_000949"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6911999.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(1679..2131)
                     /locus_tag="VBC81_000950"
     CDS             complement(1679..2131)
                     /locus_tag="VBC81_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912000.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            2383..3528
                     /locus_tag="VBC81_000951"
     CDS             2383..3528
                     /locus_tag="VBC81_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6912001.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            3530..4744
                     /gene="thiI"
                     /locus_tag="VBC81_000952"
     CDS             3530..4744
                     /gene="thiI"
                     /locus_tag="VBC81_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6912002.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            4846..6024
                     /locus_tag="VBC81_000953"
     CDS             4846..6024
                     /locus_tag="VBC81_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6912003.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    6100..6179
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            6214..6528
                     /gene="rplU"
                     /locus_tag="VBC81_000954"
     CDS             6214..6528
                     /gene="rplU"
                     /locus_tag="VBC81_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6912004.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            6535..6873
                     /locus_tag="VBC81_000955"
     CDS             6535..6873
                     /locus_tag="VBC81_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6912005.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            6895..7188
                     /gene="rpmA"
                     /locus_tag="VBC81_000956"
     CDS             6895..7188
                     /gene="rpmA"
                     /locus_tag="VBC81_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6912006.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            7404..8309
                     /locus_tag="VBC81_000957"
     CDS             7404..8309
                     /locus_tag="VBC81_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6912007.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            8318..8782
                     /locus_tag="VBC81_000958"
     CDS             8318..8782
                     /locus_tag="VBC81_000958"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6912008.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            8766..9656
                     /locus_tag="VBC81_000959"
     CDS             8766..9656
                     /locus_tag="VBC81_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6912009.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            9832..10353
                     /gene="pyrR"
                     /locus_tag="VBC81_000960"
     CDS             9832..10353
                     /gene="pyrR"
                     /locus_tag="VBC81_000960"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6912010.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            10402..11472
                     /locus_tag="VBC81_000961"
     CDS             10402..11472
                     /locus_tag="VBC81_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6912011.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            11528..13126
                     /locus_tag="VBC81_000962"
     CDS             11528..13126
                     /locus_tag="VBC81_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6912012.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            13328..14572
                     /locus_tag="VBC81_000963"
     CDS             13328..14572
                     /locus_tag="VBC81_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6912013.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            14575..15285
                     /locus_tag="VBC81_000964"
     CDS             14575..15285
                     /locus_tag="VBC81_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912014.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            15300..16547
                     /locus_tag="VBC81_000965"
     CDS             15300..16547
                     /locus_tag="VBC81_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912015.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            16676..16948
                     /gene="rpsP"
                     /locus_tag="VBC81_000966"
     CDS             16676..16948
                     /gene="rpsP"
                     /locus_tag="VBC81_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6912016.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            16958..17200
                     /locus_tag="VBC81_000967"
     CDS             16958..17200
                     /locus_tag="VBC81_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6912017.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            17332..>18698
                     /locus_tag="VBC81_000968"
     CDS             17332..>18698
                     /locus_tag="VBC81_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6912018.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLK"
BASE COUNT         6280 a         2758 c         3710 g         5950 t
ORIGIN      
        1 gaggttagcc ctgttaacaa tggtagggtt taattctgag aaatacatca aaaattataa
       61 ggaaacttta ttaaattatc aaagtgagtc gttgaatata ggtgatatta atttatataa
      121 taaaattaag caaaagattg cattgataga ttattgttac tagaaataat tttaacagca
      181 agttaggata tgataatcgg taatataaat tgtttacgta gcagaaaatt gtgactgtaa
      241 acactttgtg atagagtata ggtatcaatg attcaaggag agagtaatgt caaaacaata
      301 tgattatatc gttattggtg gaggtagtgc aggcagtggt accgctaata gggcagccat
      361 gtatggagca aaagtcctgt taattgaagg tggacaagta ggtggaactt gtgttaactt
      421 aggttgtgta cctaagaaaa tcatgtggta tggtgcacaa gtttctgaga cactccataa
      481 gtatagttca ggttatggtt ttgaagccaa taatcttagt tttgatttta ctactctaaa
      541 agctaatcgc gatgcttacg tgcagcggtc tagacagtcg tatgccgcta attttgagcg
      601 taatggggtc gaaaagattg atggatttgc tcgttttatt gataaccata ctattgaagt
      661 gaatggtcag caatataaag ctcctcacat tactattgca acaggtggac accctcttta
      721 ccctgatatt attggaagtg aacttggtga gacttctgat gatttttttg gatgggagac
      781 cttaccaaat tctatattga ttgttggggc gggctatatc gcggcagaac ttgctggagt
      841 ggttaatgaa ttaggcgttg aaacccatct tgcatttaga aaagaccata ttctacgcgg
      901 atttgatgac atggtaacaa gtgaggttat ggctgaaatg gagaaatcag gtatctcttt
      961 acatgctaac catgtaccta aatctcttaa acgcgatgaa ggtggcaagt tgatttttga
     1021 agctgaaaat gggaaaacgc ttgtcgttga tcgtgtaata tgggctatcg gccgtggacc
     1081 aaatgtagac atgggacttg aaaataccga tattgtttta aatgataaag attatatcaa
     1141 aacagatgaa tttgagaata cttctgtaga tggcgtgtat gctattggag atgttaatgg
     1201 gaaaattgcc ttgacaccgg tagcaattgc agcaggtcgt cgcttatcag aaagactttt
     1261 taatcataaa gataacgaaa aattagatta ccataatgta ccttcagtta tttttactca
     1321 ccctgtaatt gggacggtag gactttcaga agcagcagct atcgagcaat ttggaaaaga
     1381 taatatcaaa gtctatacat caacttttac ctctatgtat acggctgtta ccagtaatcg
     1441 ccaagcagtt aagatgaagc tcataaccct aggaaaagag gaaaaagtta ttgggcttca
     1501 tggtgttggt tatggtattg atgaaatgat tcaaggtttt tcagttgcta tcaaaatggg
     1561 ggctactaaa gcagactttg atgatactgt tgctattcac ccaactggat ctgaggaatt
     1621 tgttacaatg cgctaacgct atcaaagaat acaaaaaaag ctagggaacc tagctttttt
     1681 aaggtgttgt tgtattttca atagttgagg tatcggtagt agtattattt gtttgatcat
     1741 tgttagtact agatgatgtt tgatcgttat tggcagcaga atttgaactg ctactcggag
     1801 tactactagc agaggaattt tctagtgttt ggctggatga tgcccttgtc tcatcttttt
     1861 cagtagaaac ttggttacta gaattttgtc cactttctct tataatgacg gtttttgaag
     1921 tagaagaatc agtcttacta gagtctttgt gattattaat cgcattagta atagtaatag
     1981 aggcaacgat aatacttaaa acactaccga ctaatgcaat tgttttttga agtgcagaaa
     2041 aacctgtttt tatatgctta gcgggtgatt gagttgaatc agtattagtc ttagtagact
     2101 ttttctgttg tcttgaatat ccttttgaca tgacgccctc cttaaatgta catcaactat
     2161 tatatgattt tatcctaaaa aaagctaaag aaaaaataat ttaaaacatt taaataagca
     2221 acctaggtta ttaagctaag ctatgttagt gtaataggta gtgagttttg tgataaatta
     2281 tgagaattat cataaatatt gttttgggat gttcacaaac taatataaat agtaggaaaa
     2341 ttatgctata ataacactga ttgactttag aaggacagat ttatgattta ttttgataat
     2401 tcggcaacta ctattcccta tccagaagtt ttaaggactt atcaagaagt agctagtaaa
     2461 atctatggaa acccctcaag tttacatgag ctaggaacaa catcttctcg gattttagag
     2521 gctagccgta aacaaattgc tagtttactt gagcttaaag caaatgaaat tttctttacc
     2581 tctggtggta cagaggccga taattgggtt attaagggtc ttgcgtttga gaaacaacat
     2641 tttggaaatc atattattgt ttctgatatc gaacacccag ctgttaaaga gtctgctaaa
     2701 tggcttggag agtatgggtt tgagattgat tatgcgccag tagatgataa aggatttgta
     2761 gacgtagaag cactggtcaa attaattaaa ccagaaacga ttcttatttc aattatggca
     2821 ataaacaatg aaattggttc tattcagcca ataaaagcta tttcagactt gttgtcagat
     2881 aaacctacta tatcgtttca tgttgatgct gttcaagcaa taggtaagat accgactaaa
     2941 gattatttga ctgagcgagt tgattttgca tctttttcta gtcataaatt tcacggcgtt
     3001 cgtggggttg gctttctata tattaaagaa ggaaaacgta tttcaccatt actaacaggt
     3061 ggtggacaag aatctgattt acgttcaact actgagaatg tcgcagggat tgcagctact
     3121 gctaaagctt tacgaatggt tatggacaag gaagtagtag ctattcctaa aataagtaaa
     3181 atgaaaacca ttattcatga tgaattagcc aaatatgagg atattacgtt attttcgggt
     3241 aaggaaggtt tttcacctaa cattatcacg tttggtatca agggtgtccg aggagaagtt
     3301 cttgtccatg cctttgaggg acatgatatt tttatctcga caactagcgc ttgctcttct
     3361 aaagcaggta aaccagcagg aactcttata gcaatgggaa tttcaactaa gttagcccaa
     3421 actgctgtac gtatcagctt agatgatgat aatgatatgg gacaagtaga gcaattttta
     3481 actattttta aacaagttta tgaaaaaact aagaaagtta gaggataaga tgcagtattc
     3541 agaaattatg attcgttatg gagaactctc tactaagaag aaaaaccgta tgcgcttcat
     3601 caataagtta aaaaataata tggagcatgt actctccatt tatccagatg tttcagtaaa
     3661 aacagatcgt gatagaggac atgtatatct caatggtaca gattatcatg aagttgcaga
     3721 gtccttaaaa gagatttttg gtatccaagc tttttctcca tcttttaaag tagaaaaaaa
     3781 tgttgataca ttggtaaaag ctgtccagga aattatgact tccgtttata aagatgggat
     3841 gacttttaaa attaccgcaa aacgtagtga ccactcattt gaattggata gccgtgctct
     3901 aaatcatact ttaggagatg ccgttttttc agtcttgcca aatattaagg ctcagatgaa
     3961 gcaaccagat atcaatctta aagtcgagat acgagatgag gctgcttata tttcatatga
     4021 ggatattagg ggtgcaggag gattaccggt aggaacatct gggaaaggga tgctgatgtt
     4081 gtctggtggg attgattctc cggtagcagg ttacctagcg ttaaaacgtg gtgtagatat
     4141 agaagcagtc cattttgcaa gtcctcctta tactagccca ggtgcattga aaaaagcaca
     4201 tgatttaaca cgtaaattga caaaatttgg tggtaatatt caatttattg aagttccatt
     4261 cacagaaatt caagaggaaa ttaaggaaaa agctcctgaa gcctacttga tgacgttaac
     4321 acgtaggttt atgatgcgta ttacagatcg tattcgtgag gaccgaaatg gtcttgttat
     4381 tattaacggt gaaagtttag ggcaggtggc aagccaaacg ctagaaagta tgcaagctat
     4441 taatgctgtc actgcaacac cgattattcg tcctgtggtc acgatggata agctagaaat
     4501 tattgatatt gctcaaaaaa tagatacttt tgatatttca attcaaccat ttgaggattg
     4561 ctgtacaatt tttgcaccag atcgcccaaa aactaaccct aaaattaaga atacagaaca
     4621 gtatgagaaa cgtatggatg tagaaggtct ggtagagagg gcagttgcag ggattatggt
     4681 aactactatt caacctcaag cagatagtga tgatgttgat gacttgattg acgatttatt
     4741 ataatgaatt tttaaactac agatttactc tgtagttttt tattaaaatg attgtgctgt
     4801 cataaggtta taaaaaatgc tataatacga ataaaggagg caagtatggc aagaatccct
     4861 tatcaaaaaa cacttatttt ttgtgtagcc gttattattg ctatttttat tttggggtta
     4921 tctaaggatt tagcacaatc taaagggcaa aaggttgcaa ataataatac tgtaaaaaca
     4981 gcacgtgtgg ttgcaaacgg agatattttg ctgcacgatg tactatatgc cagtgcgcgc
     5041 caacccgacg gcacttataa ttttactcct tattttaaag aggtaaaatc ttggattgag
     5101 agcgctgatt tagcgattgg tgattatgaa gggacgatta gttcagaata tcctttagcg
     5161 ggctatcctt tatttaatgc tcctaatgag attgcaacta ctatgaaaga aactggatat
     5221 gatgtcgttg acttagcaca taatcatatt ctggattcac aactggcagg tgctatcaat
     5281 actgtcaaga cttttaatcg tctgggattg gatactattg gggtttattt aaaggataga
     5341 aataaagaag atatcttaat taagcgtgta aatggtatta aaattgctat tttaggttat
     5401 tcgtatggct acaatggtat ggaagctaat gtttcaaaga gtgactatga aaaacatatg
     5461 tctgatttag atactaaaaa gattaagcaa gatattaaaa aggctgaaaa agaagcagat
     5521 atcaccattg tcatgccaca gatggggata gaatatcaaa aaaaacctac tacagaacaa
     5581 gtaatacttt atcatagtat gattaaatgg ggggcagata ttatattcgg aggacaccct
     5641 cacgttgttg aaccgtctga agtgataaaa aaagatggcc agaaaaaatt tattatttac
     5701 tctatgggaa attttatttc aaatcagcgt ttagaaacgg ttgatgatat ctggacagaa
     5761 agaggattat taatggatgt aaccatagag aaaaaaggtc aaaaaacagt aattaaaaaa
     5821 gttaaggcgc atccaacact agtagaagca aaagcaaatg gccgttatgt tgaagaaggc
     5881 tttccagctt atgattatag gacgctagtt ttagaggatt atattgaagg tggtaagaaa
     5941 cgttctacaa ttgatcagac tatgaaagat aaagttgata aagcatataa ggaaatgaat
     6001 gagttagtta atttgaagtg gtaaagttga taattttatt ttgttttatc ttgcataaaa
     6061 aaagagttta tgataaaatt tgaatgttga ctaaatgcac atcctgtgca accgcgcgaa
     6121 aatcgttaga ttaagtaggg caaggctctc acgatttata ggcgagtctt cacagaggga
     6181 aacctcacaa gaaattttta taggaggtgc atcatgagca catacgcaat catcaaaact
     6241 ggtggtaaac aagttaaagt tgaagtagga caagctatct acgttgaaaa acttgacgtt
     6301 gaagcaggag cagaagttac atttaacgaa gttgttcttg ttggtggtga aacaactaaa
     6361 gttggtactc cagttgttga aggagctact gttgttggta ctgttgaaaa acaaggcaaa
     6421 caaaagaaag ttgtttctta caaatacaaa cctaaaaaag gtagccaccg taaacaaggt
     6481 caccgtcaac cttatacaaa agttgttatc aacgcaatca acgcttaatt tttcatgatt
     6541 aaagcaactt ttactcgtaa tcaatcaggc tatctttata gtgctgaaat cagcggtcat
     6601 gctggtagtg gcgagtacgg ctttgatgtg atttgtgcag ctgttagtac tttatcaatt
     6661 aattttatta attcattaga ggctttaaca acctgccaag cgcaattgat tatcaatgac
     6721 gttgaaggtg gttatatgaa gatagattta tcttctatac cacaacataa agaggataag
     6781 gttcaattat tatttgagtc ttaccttctt ggaatgacta atctgtctaa agattcttct
     6841 gaatttgtta gtacagtagt catgacacac taatttaaga gaggacacaa cattatgtta
     6901 aaaatgaatc ttgctaactt gcaacttttc gcccacaaaa aaggtggagg ttctacatca
     6961 aacggacgtg attcacaagc aaaacgtctt ggagctaaag cagctgacgg acaaacagta
     7021 tcaggtggat caattcttta ccgtcaacgc ggtacacaca tctatccagg tgctaacgtt
     7081 ggacgtggtg gggacgatac actttttgct aaagttgaag gtgttgttcg tttcgaacgt
     7141 aaaggacgcg ataaaaaaca agtgtctgtt tacccaatcg caaaataatg cctagttaag
     7201 actttatgac tattcataag gtcttttatt attgtcagag ctttcatttt gtaacaaaag
     7261 acaatatgtt aagagattta tcattaatac tcataaactt tgttgttctt ttatagtgaa
     7321 atagtcctaa ttcatgaaga attttccgca aggatagtta ttttttggta taatgaaggg
     7381 tactaaaatg aggaagtgat aaaatgaata ttcaacaact gcgttatgtt gttgctatcg
     7441 caaatagtgg tacttttcgt gaagcagctg caaaactatt tgttagtcag ccaagtttat
     7501 ctgttgccgt tcgtgatttg gaaacagaat tagggtttca gatttttacg cgtacaacta
     7561 ctggtgcagt gttgaccaat caaggcatga ctttttatga gaatgcttta gaagttgtga
     7621 aaagctttga ttcattcgaa aaacagtttt cgcaatctga agctacagag caagaattct
     7681 caatagcaag tcaacattat gattttctac ctcctttaat cacagcgttt tcaaaatgta
     7741 atgataattt tagctatttc cgtattttcg aatcaacaac aatacgtatc ttagatgaag
     7801 ttgctcaagg gaatagcgaa ataggtatca tttatatcaa tagtcaaaat aaaaaaggtc
     7861 tacttcagcg gttggacaaa ttggggttag agtttgtaga gcttattcct tttaaaactc
     7921 atatttatct tgggaaagac catcccttgg ctagtaaaac atctctgata atgacagatt
     7981 tagaaggatt accaaccgta cgttttacac aggatagaga tgattatcgg tattactcgg
     8041 agaattttgt ggaagtactt gatagttctg ttacctataa tgttactgat agagcaacat
     8101 taaatggtat tttagaaaga acccaagctt atgcgacggg ctcaggattc ttggatagcc
     8161 gcagtgtgaa tggtatcact gttattccat tagaagatca tctagataac caaatgattt
     8221 atatcaaacg aaaagatcgt aatttatctc agatggcttt aaaatttgtt gctgttatgg
     8281 aagagtattt taagggattt aattcatagg agaagtaatg agaaagatta ttataccaat
     8341 aataactatt ttgttgattg ctttagatca actatctaag ttatggattg ttaaacatat
     8401 tgaactaaac caaattaagg aattcattcc aaatatcgtt agtctaactt atctgagaaa
     8461 ttatggtgct gcattttcta ttttgcaaaa tcaacaatgg ctttttacct taattacaat
     8521 ctttgttgtt tgtgttgcta ttatttattt gatgaaacat attaatggaa gttattggtt
     8581 attaatttct ctaaccttaa tcatctctgg tggattagga aactttatag atcgcttaag
     8641 attaggttat gtcgttgata tggtgcattt agattttatt aattttgcta tctttaatgt
     8701 agcagactca tatctgacaa tcggtattat ttgcttaatg attgcacttt ggaaagagga
     8761 aagtaatgga aatcacaatt aaaatagcag gagttaggct agataaagct ttagctgata
     8821 attctgaact gtcgcgtagt caagctaatg aagaaattaa aaaagggatt gtgttagtaa
     8881 atggacaagt aaaaaaagcg aaatatactg ttcaagaagg agatcggatt acttttgata
     8941 ttcctaaaga ggaagtacta gattatcaag ctgaaaatat ccctttagat attatctatc
     9001 aagatgatga cgttgctgtg gttaataagc cgcaagggat ggttgttcac ccttctgcag
     9061 gccacagttc gggcacctta gttaacgcct tgatgtatca tattaaggat ttatcatcaa
     9121 ttaatggcgt tgttcgccca gggatcgttc atcgtattga taaagatact tctggtttat
     9181 tgatggttgc aaaaaatgat agagctcacc aagtgttggc tgaagagttg aaagataaaa
     9241 aatcgcttcg taaatattta gcaattgtgc atggtaattt accaaatgat cgaggcgtga
     9301 ttgaggctcc gattggtaga agtgataaag atcgtaagaa gcaagccgtg actgctaaag
     9361 gtaagccagc aataactcgt tttcatgttt tagaacgttt tggtgattat accttagttg
     9421 aattgtcatt ggagacaggt cgaacgcacc aaattcgggt tcatatggct tatattggcc
     9481 atcctctagc cggtgatcct gtctacggtc cccgcaagac tttagggggt aaaggacagt
     9541 ttttgcatgc acaaacacta ggttttacgc accctagtaa cggagagaat ttgatttttt
     9601 cagtagaggt tccggaaatt tttcagacaa ctttagacaa gttgagaaaa aattaagttt
     9661 ttcttgaaaa atacttttac catttgctat aatataagca aatcattcct ttaaccttat
     9721 ccagtgagat agttaaggag aaacttcaga ctaaatcaat gcctgagcgg ctttgtcatt
     9781 ctagagagtc tccttttgaa ggggctcttt tatcttagag aggatactgc aatgaaaaga
     9841 aaagaaatta ttgatgatgt gaccatgaaa cgtgctatta cacgtattac ctacgaaatt
     9901 atagaacgta acaaaaattt agataatatt gttttagcag gcattaaaac aagaggtgtt
     9961 tttttagcca aacgcattca agaacgtttg aagcaattag aaaatttaga tattcccgtt
    10021 ggagaattgg atactaaacc tttccgtgat gatatgaaag ttgaagttga caccacaact
    10081 atgccagttg acatcactga taaagatatt atattaattg atgatgttct ctatacaggt
    10141 cgcactattc gagctgctat tgataatctt gttagtttgg ggcgtccgag tcgtgtcagt
    10201 ttagctgtat taattgaccg tgggcatcgt gaactaccca ttcgtgcaga ttatgtgggg
    10261 aaaaatattc caacaagtca atttgaagaa attctagttg aagtgatgga acatgatggt
    10321 tacgatcgtg tgagtattat tgatccgagt taagtagacg actattattt acgatgattt
    10381 tatagaaggg gaagaaccac aatggcaaaa aaattgttaa ttctagagga tggtacggtt
    10441 tttgaaggac tatcctttgg ttcatctctt gatgtaactg gcgaattagt tttttgtacg
    10501 gggaataccg gctatcagga aataataacg aatccttcac ataatggaaa aattttagtt
    10561 tttacctcac cgctaatagg aaattatggt attcatcgta gttactctga agccattatt
    10621 cccacttgtt taggagttgt tgttgcagag tattccagat gtgtaagtag cgatactagc
    10681 aagatgaatc tagatgaatt tttgaaaatg aaaaaggttc ctgcaatgtc gggggtcgat
    10741 acaaggtatt tgatgcaagt tattaaagaa aaaggatttg taaaagctac tttagcagaa
    10801 gcaggtgatg ttttatcaca tttacaagat caattaatag cgacagtatt accgacaaat
    10861 aatgtggaac aagtttcgac aaaaacagct tatccgtcgc ctgctagcgg tcgaaatatt
    10921 gttgtacttg attttggcct taaacattct gttttgagag aattgtctaa acgtcagtgt
    10981 gacgtgactg tcattcccta caatacgtct ttagaaggta tcaaaaatct atacccagaa
    11041 ggtatcatat tatctaatgg tccgggaaat ccagaaaagc ttcaggaagt cttaaacact
    11101 attaaagaat tacaaaaatc tgttcctatg ttaggtattg gtttggggca tcaattaatt
    11161 gccatggcaa atggtgcaga aattatgaga ctcccagttg ctaagaaggg gccaaattat
    11221 cccatgagag atattgctac ggggcgttta gaaacagtta ttcagtttaa tcattttacc
    11281 gttaaccggc taaatcttcc gcatgattta ctggtaacac acgaaggttt gaatgatcaa
    11341 gagattgttg ctctaaggca ccgctcattt ccagtgatgt ccgtgcaatt ttacccagag
    11401 gctgctccag gccctcatga tgtgacctat ttttttgatg aatttttaga aatgattgga
    11461 tcacgcgctt aaacataagg tattatttaa agtcagtcgg tgagctgatt aataaggagg
    11521 tgcgtatatg ctaaatacaa aggataaaca gcctattttg cttattattt tcggacagaa
    11581 aatgaaagaa tatgacgatt taattagatc aactgttgag aggttatcta aaaggaatag
    11641 acctgttagt attcttatct cgaaggagct aaaccatgtg attcaccatt atgatgcaca
    11701 atttgaaaat cttgagacgc ttgtggggta tataaataca catgaagata gtttgattta
    11761 tcccctttgc ttagataaaa atcatgagct atttcaagag tttttagcgt ggcagagtca
    11821 ttattcaagg cagtatatca gtgataattt atcagaaaaa agtgttcagg caatagacaa
    11881 taatcaagac tttgtgcaag tagattgttt agtaatgaga gactctttga ataattgttt
    11941 atatgtctct gatttagaat atattgaatc taataaaaca actggtaagt ctctagcgat
    12001 tgtaccaagt caaactttat ccgatgctgc taggcaaaca attcgagatg ttgcatttga
    12061 tgtgtgtcga aaggcaaaca ttattggagt atgttacttt agtttcttaa tagatttgaa
    12121 cagtttggat tatcatataa ttagcttatc atctggtttg tcttatcaaa gcattttatt
    12181 tgagactatt acgacctatc ctgttttaga gatagcaact aaattgacag tgggatatac
    12241 ttttagtcaa ctgaagcatt cttattaccc aaatacctct gcctttttag agccgcaatt
    12301 agactatgta gctaccgtat cttttagttt tgaaaaagtt gactatatct tttttgctcg
    12361 taatatagag caattatttt taaatttact cgaggctagc agtcatgatc attttccatt
    12421 tctgagtgat attagcgagg aagatttgat gtttgctttg attcagaaaa aagaaaatcg
    12481 tctagcttat cttttagagg cttttagacg tggctttgat ttgtatgatt tatcctcagt
    12541 tactaaaatc aacccttttt acttagataa atgtttacat attgtagagt tgtatgaaaa
    12601 cctgaataaa agccaatata atgtagatat ttacaaggat gctaaaaggt atggtttttc
    12661 ggatgactat attgctagta gttggcaaat atctttgata gacatgttag aatatcgaaa
    12721 aaaacatagt gtcgcacctg ttctgaagca agtcgaacaa agttctggtg ttctaactgg
    12781 ccatcaaatt cagtattttc gaagctatga ttggcattca gactacagtt ctagcggttg
    12841 tcaaaaggct ttgataatgg tagacaaggg ttattcccta gtaaaattaa atgagttaat
    12901 taagcaaatc aaacaaactc atttagaatt attaattgtt accaatcaac ctctcctcat
    12961 agaacaatta aatgatacat ctataatttt tgacactata ggtattgaga ccatattaac
    13021 aattatgggt atagaagagg tggaagaagt gtatttattg ggagaaagtc aagcagacat
    13081 gttttcaaaa atgaaaaatt tttctatctc ttgtcagtct atttagcaat tcttaataaa
    13141 agctgagata tagatctcag cttttatttg tggtaaaaag taaagtactt tcaattttgt
    13201 aagttctttc tatgagcaaa atagtatact taatgagtaa aaatattatt tttaaagaat
    13261 tttcttgaca atattattta aaaataatac acttgttcta tattaataat acaaacagga
    13321 gatggacatg agtaaacgac aaaatttagg gattagtaaa aaaggagcaa ttatatcagg
    13381 gctctcagtg gcactaattg tagcagtagg tggcttttta tgggtacaat ctcaacctaa
    13441 taagagtgca gtaaaaacta attacaaagt ttttaatgtt agagaaggaa gtgtttcgtc
    13501 ctcaactctt ttgacaggaa aagctaaggc taatcaagaa cagtatgtgt attttgatgc
    13561 taataaaggt aatcgagcaa ctgttacagt taaagtgggt gataaaatca cagctggtca
    13621 gcagttagtt caatatgata caacaactgc acaagcagcc tacgacactg ctaatcgtca
    13681 attaaataaa gtagcgcgtc agattaataa tctaaagaca acagggagtt ttccagctat
    13741 ggaatcaagt gatcaatctt catcatcatc acaaggacaa gggactcaat cgactagtgg
    13801 tgcgacgaat cgtctacagc aaaattatca aagtcaagct aatgcttcat acaaccaaca
    13861 acttcaagat ttgaatgatg cttatgcaga tgcacaggca gaagtaaata aagcacaaaa
    13921 agcattgaat gatactgtta ttacaagtga cgtatcaggg acagttgttg aagttaatag
    13981 tgatattgat ccagcttcaa aaactagtca agtacttgtc catgtagcaa ctgaaggtaa
    14041 actccaagta caaggaacga tgagtgagta tgatttggct aatgttaaaa aagatcaggc
    14101 tgttaaaata aaatctaagg tctatcctga caaggaatgg gaaggtaaaa tttcatatat
    14161 ctcaaattat ccagaagcag aagcaaacaa caatgactct aataacggct ctagtgctgt
    14221 aaattataaa tataaagtag atattactag ccctctcgat gcattaaaac aaggttttac
    14281 cgtatcagtt gaagtagtta atggagataa gcaccttatt gtccctacaa gttctgtgat
    14341 aaacaaagat aataaacact ttgtttgggt atacaatgat tctaatcgta aaatttccaa
    14401 agttgaagtc aaaattggta aagctgatgc taagacacaa gaaattttat caggtttgaa
    14461 agcaggacaa atcgtggtta ctaatccaag caaaactttc aaggatgggc aaaaaattga
    14521 taatattgaa tcaatcgatc ttaagtctaa taagaaatca gaggtgaaat aatcgtgaca
    14581 actaatcgaa aagaactgat tagattgcat cagattgtta aaagttatca aaatggggat
    14641 caaaaacttc aagtcttaaa gaacattgat ttgacggttt atgaaggtga atttcttgca
    14701 atcatggggc catctggctc agggaaatcc acactaatga atattattgg cttgttggat
    14761 tccccaactt caggagatta tagtttaaat ggtaaacgtg tcgaagaact aagtcaaaca
    14821 aaattagctc aagttcgaaa taaggaaatc ggttttgtct ttcaacaatt tttcttgtta
    14881 tctaaactaa cggctttgca aaatgttgaa ttacccttaa tttatgcggg tgtagctcct
    14941 aaaaagcgta aaaatttagc aaaacaattt ttagataaag ttgaattgag agagcgtatg
    15001 aaccatctgc caacagaatt atctggggga cagaagcaac gtgtagcaat cgcgcgtgct
    15061 ttggtgaata gcccatctat tattctagca gatgagccga ctggtgcctt ggatacaaag
    15121 acgggtgagc aaattatgca gttcctaacg gaattaaatc aagaaggtaa aaccattatc
    15181 atggtaactc atgaaccaga aatcgccgat tatgcgacac gtaaaattgt tattcgtgat
    15241 ggcgaaatta cagcagatac tactgatagt attagaattg attaagggga gattcgatta
    15301 tggaaaattg gaaatttgcc ctatcatcga ttctagggca caagatgcgt gcttttctga
    15361 ccatgttagg gattatcatt ggggttgcat cagttgtgtt aattatggcc ttaggtaagg
    15421 gcatgaaaga ttcggttaca aatgagatta caaagtctca gaagaatctt caaatttatt
    15481 ataaaacaaa agaagaccaa aaaaatgagg ataactttgg agctcaaggg gctttcatgc
    15541 aaggtagtga taccaaccgt aaggaaccaa ttattcaaga atcatggtta aaaaagattg
    15601 ctaaggaagt agatggtgtt agtggctact atgtaaccaa tcaaacaaat gctccagtag
    15661 cttaccttga aaaaaaggca aagacggtta atataacagg aattaaccgc acttaccttg
    15721 gtatcaaaaa gttcaaaatt aaaagtggtc gtcaattcca agaggaggat tataaccaat
    15781 tctcgagagt tattttacta gaagaaaaat tagctcagag attatttcag acaaatgaag
    15841 cagcgttaaa taaagtggtg actgtaaaaa ataaatctta cttagtagtg ggggtttatt
    15901 cggatccaga agctggctca ggtttatatg gaagtaattc tgatgggaat gctattttaa
    15961 ccaatacaca gttagcttcg gagtttggtg ctaaagaagc agaaaacata tattttcatc
    16021 ttaatgatgt gtcgcaatcc aatcgcattg gtaaagaaat aggcaaacgt ttaacagaca
    16081 ttagccatgc taaggatggg tactatgata attttgatat gacatctata gttaaatcta
    16141 tcaataccca agttggtatt atgacaggtg ttattggagc gattgcagca atttctctcc
    16201 tagttggtgg tatcggagtg atgaatatta tgttagtctc tgtaactgag cgcacgagag
    16261 aaattggcct tcgtaaagcc cttggggcaa ctcgacgtaa aattttagct caatttttaa
    16321 ttgaatcaat ggtcttaacg attttgggtg ggttaattgg gttattacta gcctatggag
    16381 gtacgatgtt aattgctaat gcgcaagata agattactcc ttctgtgtca cttaacgtgg
    16441 ctataggcag tttaattttc tcagcattta ttggaattat atttggttta ttacctgcta
    16501 ataaagctag caaattaaac ccaattgatg ctttacgata tgaataaaaa aacatgtact
    16561 ctgtcaagat tttttcttga cagagtttgt atttatgtta tacttagtaa ggtaaagttg
    16621 ttagttaagc tatcaacgac caaatataaa gaaaaagaaa agagactatt taaaaatggc
    16681 agtaaaaatc cgtttaactc gtatgggttc taagaagaaa cctttctacc gtatcaatgt
    16741 tgcagattca cgtgcaccac gtgacggacg ttttattgaa actgttggaa catacaaccc
    16801 attagttgca gaaaaccaag ttacaatcaa agaagaacgt gttcttgaat ggttatcaaa
    16861 aggtgcacaa ccttcagata cagttcgtaa ccttctttca aaagctggag ttatgactaa
    16921 attccacgat caaaaattct caaaataata aaagcctatg gataccattg aaaatctcat
    16981 tattgctatt gtgaaacctt tgatttcaca accagatcag ttaaccataa aaattcagga
    17041 tggcccagaa tttttagagt accatcttga tttagatacc caagacatcg gtcgtgttat
    17101 cggcaaaaag ggtcgtacaa ttacagcgat aaggtcgatt gtctattcgg taccaacaca
    17161 agggaaaaaa gtaagactca tcattgatga gaaagaataa tttattctaa aagataggaa
    17221 cttcctgtct tttttgttat atctacacaa aaattgtgag ttttttagtc ttttagaata
    17281 taatggatgt gaaagggaat tttctgattt tttattaagg aggataagat attgctattt
    17341 gattttctcg aagaaaaaat aaaactgaaa gcatacctga tgaccgcctt acttgatgat
    17401 ggagaaatta accttgatca ctttaaaaca gaatatgctt tgagtgaaaa gattttacaa
    17461 ggtcttattt cggaactaca aatactttat tctgatttct accttgttgt caaaagtaga
    17521 acaatggtat tgttccatta cgaaacactt tctaagctag agactttgca taccatttat
    17581 cgagagtcta atacgttaca gtttttggca tattttattg ctcctcaaaa tgtcagtttt
    17641 tctgaattca cggctaagaa attcatatcc gtcgcaagtg cctaccgaat aaagaaaaac
    17701 tgtaaacttt atttggagag tgttaagtta tcaatacaaa aaaataaagt gacaggtcca
    17761 gaatatcaga ttaggctgct gattgctttg ttacagtatc attttggttt tattatttat
    17821 ccttttgatg ctacaagtga ggtaatgata agggatttta ttgaatactc gaattttaaa
    17881 gggtgtgact acgaccttaa agacttatct gaagaatatt actattttta tattttggtg
    17941 aatttagttt ggaaaagaaa agaatataaa gtagaaattc ctcaaacaga atcctttaat
    18001 caactaaaaa aatctattct ctacatggag ttatccaaag cagctcaaaa gagtattgct
    18061 atacatcttg gtatagagtt gaatgaaggt ttacttgatt atctatattt agtgttttgt
    18121 attagtgatg gtcctctatt tagcaatcga gatgatatca atatcaacga agaatttatt
    18181 tttgcttctt ataagatatc aaagtctaaa gcctttaaga atttttgtgg tcgattattg
    18241 ggtagagaat ttattgactc gcagcatttt aaagatatta atgcctattt tttgagacat
    18301 tttatttgtt attgttatta ttttattcct gatttttatt ttttaaatac atcaaggctg
    18361 agctattcta aggatttata tcacctatta gataaaggtc tggctgatat ttttaacctt
    18421 aaaggaggga atttaacttt ttcaaaacat gagacagtgc tactaacaat gcaattatct
    18481 aacttaatag agactttctt agctcctctt tctgtatatg taatttcatc tagtaatatt
    18541 agattacaaa cctatcaagt aatgttaaac caatatttta catctaagat agcggaattc
    18601 ttttttgtaa actatcaaac gactcagata gatgaaaaat tactaaaaaa agcagatatc
    18661 attattgctg agagacgcta tatatcttct ttaaaaaa
//
LOCUS       DATRNX010000035        17795 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRNX010000035 DATRNX010000000
VERSION     DATRNX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17795
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            64..1803
                     /locus_tag="VBC81_000969"
     CDS             64..1803
                     /locus_tag="VBC81_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6912019.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1815..2549
                     /gene="ltdR"
                     /locus_tag="VBC81_000970"
     CDS             1815..2549
                     /gene="ltdR"
                     /locus_tag="VBC81_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6912020.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2705..4189
                     /locus_tag="VBC81_000971"
     CDS             2705..4189
                     /locus_tag="VBC81_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6912021.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
     gene            4391..4963
                     /locus_tag="VBC81_000972"
     CDS             4391..4963
                     /locus_tag="VBC81_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6912022.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            5239..6090
                     /gene="ylqF"
                     /locus_tag="VBC81_000973"
     CDS             5239..6090
                     /gene="ylqF"
                     /locus_tag="VBC81_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6912023.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            6077..6838
                     /locus_tag="VBC81_000974"
     CDS             6077..6838
                     /locus_tag="VBC81_000974"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6912024.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            6859..7407
                     /locus_tag="VBC81_000975"
     CDS             6859..7407
                     /locus_tag="VBC81_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6912025.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
     gene            7647..8609
                     /locus_tag="VBC81_000976"
     CDS             7647..8609
                     /locus_tag="VBC81_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6912026.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            8606..9580
                     /locus_tag="VBC81_000977"
     CDS             8606..9580
                     /locus_tag="VBC81_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6912027.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            9577..10338
                     /locus_tag="VBC81_000978"
     CDS             9577..10338
                     /locus_tag="VBC81_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912028.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            10400..11428
                     /locus_tag="VBC81_000979"
     CDS             10400..11428
                     /locus_tag="VBC81_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6912029.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            11566..12408
                     /gene="dprA"
                     /locus_tag="VBC81_000980"
     CDS             11566..12408
                     /gene="dprA"
                     /locus_tag="VBC81_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6912030.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            12503..14623
                     /gene="topA"
                     /locus_tag="VBC81_000981"
     CDS             12503..14623
                     /gene="topA"
                     /locus_tag="VBC81_000981"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6912031.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            14760..15461
                     /locus_tag="VBC81_000982"
     CDS             14760..15461
                     /locus_tag="VBC81_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912032.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            15473..>17795
                     /locus_tag="VBC81_000983"
     CDS             15473..>17795
                     /locus_tag="VBC81_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912033.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNSKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYHRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAER"
BASE COUNT         6041 a         2985 c         3100 g         5669 t
ORIGIN      
        1 taatgaaatg aatcatttta tacctatgtt ataacggact aattaatact ttggagagat
       61 ttaatgacac tctttttaat tatgatggag cgagcggggt taatcatatt attagcctat
      121 gcctttgtgc atattccctt tataaaacaa actttgaagc aacccgaatt aaaaaaacac
      181 cagtatattc tgcttatctt atttagctta tttgctatta tttctaattt tacaggggta
      241 gaaattcagt cagatttatc tatcattcca caaactctta atcatatagc agatcaatca
      301 tcagttgcca acacacgtgt tttaaccatt ggtgtatcag gtctaatcgg tggccccata
      361 gtcggtatta tagttggtct cttatctgta ttcgttaggt acttgcaagg aggattagcc
      421 cctcatattt atgtcatttc ttccttacta attggtttat gctctgggtt atcaggcaat
      481 tatcttcgta aaaattataa taaaattcgt gttttagatg caatggttgt tggtttcggg
      541 atggaaattc tacaaatgat ctgtatcctc atttttagtg ttgactttaa tcaggcgctt
      601 cgattagtat cttttatcag catgccaatg attttaagta atactttagg tttaggtata
      661 tttatatcca ttatttcatc tactcaaaaa ttagaagaac atgccaaagc atttcaaacc
      721 catcaagtac ttgaattagc aaatttaacc cttccttacc ttagaaaggg actgacaaca
      781 gaatcttgtc aaccagtagc tgaaattatt cataaacata tggatgtgag tgctgttagc
      841 ctgactagtc aatcagccat tttagcctat gtaggagatg gagctgacca ccatcttcct
      901 aatactcaaa tattaacaaa gcttgctaaa agagcaatag atactggtaa agttagtgtc
      961 gcaactgata aaagtgaaat cgaatgcgac cataggaatt gcccacttag ttcagcaatt
     1021 gttattccat tacacatcca cgatgttata gtgggtactt tgaaattata tttttcagac
     1081 gcacaacata tgacttacgt tgaccgtcaa ttagctgaag gattaggcaa tatcttttcc
     1141 actcagttag ctttgggaca agctgaggaa gcaacaaggt tactacagga tgcggaaatg
     1201 aagtctctcc aagcgcaggt taatccgcat tttcttttta atgcactcaa tactatttat
     1261 ggtttaattc gcatggatag cgaaaaagca agaaagctcg tccaagattt tagcaaggtt
     1321 attcgtgcta atcttcaacg ggcaaagcaa aatttaattc ctctccatga tgaattggaa
     1381 caggttaacg cctatttagc ccttgaagaa gcaagatttc ctaacatggt ggcctttaat
     1441 ttagacaacc aaacaaattc agatgacaac ttaatgattc ctccctttac attgcaggtc
     1501 ttgattgaaa actcttataa acacgctttt aagcatatca ataaaaataa ccagctaaag
     1561 gttactattg caagaaataa tgataggctt catatcattg tccaggacaa tggtattggg
     1621 attccaaaag aaaaattaat aacgcttgga aaaaaaactc aaatttcaaa aaaaggttct
     1681 ggtacagcta ttgagaatct cgttcgtcgc ttgaatatta tttatgatgg acaagcaagt
     1741 ttaaaatttg aatcaaatga ttcaggaact tgtgctattg ttaacatccc cattaaaaat
     1801 tagaaaggta ttgtatgaaa gttttagtag ttgatgatga accagttgca cgtaacgaat
     1861 taatttacct tcttaataag tatgattcta acctcgttat agcagaggcg catgatatgg
     1921 ctactgcatt agctatttta cttagagaaa cttttgatgt agcactgtta gatatccatc
     1981 tcagagatga ttctgggttg caattagcag agtatatcaa taaaatgccc aaaccaccat
     2041 tattgatatt cgcgactgct tatgatcaat atgctattca ggcttttgag caggatgcgc
     2101 gtgattattt gttaaaaccc tatgagtttg ataggttaaa gcaagctatg gatagagtaa
     2161 aaggagcgct aagtacatct acaattatag agagcgtagc ttccggtcct ctcttcaagc
     2221 aacagtatcc attgacagta gaagatcgaa tctatctggt gtcggcggat gatatccttt
     2281 tgattgaagc tatgcaagga aaactgatta tacaaacacc tgataaaaat tatgaaattg
     2341 atggctctct acaacaatgg caagataaac taccatcatc tcaatttgta cgggtacatc
     2401 gctcttacat tgtgaatatt aatgctatta aaacgattga accttggttt aaccaaacac
     2461 ttcagttaca cctttgtaat aaaataacag ttcctgttag cagagcaaat gtaaaacccc
     2521 taaaacaaat gttaggcata tctacctaac taatgaaaag ttattcatca taaaacttag
     2581 actattcatt tctaaaaaac aaccattcat cttgaaaata ggaaatttca atttttcaat
     2641 gctatactaa agtcatgaaa acgcttaata aaattaagca aaaaaatttt taggaggcag
     2701 tgatatggta acatttttag gaggggtagc ccttttaatt gttggttatt tcacgtacgg
     2761 tagatacatt gaaaagaatt ttcaaattga tgaaaatcgc caaacacctg cagaagcatt
     2821 aagggatgga tatgattttg ttcctatgcc aaaatggaag aatgggatga tcgagttatt
     2881 gaacatcgca ggaactggac ctatctttgg acctatttta ggagcactct atggtccagt
     2941 tgcttatatc tggatagtct taggatgtat ttttgcaggg gctgttcatg attatatgat
     3001 tggaatgatt tctcttagaa ataatggtgc atatcttcct gaattagcta gtcgctatct
     3061 tggaaaatca atgaagcatg tgatcaacat tttttcaatg ctattactta ttttagttgc
     3121 aaccgtattt gtggtaactc cagctaatct tatcctgagt attttaccag ctgggacact
     3181 atctttacct tggataatcg gtcttatttt cgtttattac cttatttcta cagtattacc
     3241 tattgataaa gcacttggta aagtttatcc tgtattctgc gtcatcctaa tggttagtac
     3301 agcagcagtt ggcttccgtt tattaactgg tggctttgat atgccaaact taacttttga
     3361 aacctttaaa aatatgcatc cagctggact tgggatattc cctgctttat tctttaccat
     3421 ttcatgtgga gccatctctg gtttccatgc tacccaagca cctatggtct caagaacaac
     3481 tgttaatgag agagaagggc gtttcacttt ctatggtatg atgattgcag aaggagtaat
     3541 tgcgatgatt tgggcaggtg cttcaatgag cttgttcaaa ggtcaaaatc tctatgaaat
     3601 gattgctgct ggaacacctt ctgcagttgt caatcaagta atgttaatgt tactcggtag
     3661 tgtaattgga actatcgcta tcattggtgt tattgttctc cctgtttcat ctggtttatc
     3721 agctttccgt agcttacgta ctatcgttgc tgattatatc catgtcaaac aagatactct
     3781 tccaaaaatc tttgctgtaa ctatcccatt atatgttatt tcatttgtcc ttacacacgt
     3841 tgattttaat ctcctatggc gttactttaa ctgggcaaat caagtaactg ctgttattgg
     3901 tttattagtt gctacaagat atctcatcct aaaaagacga aattattggg taacatttgt
     3961 accagctatg ttcatgttgt atgcagtagt tgtttatatt ttatctcaac caattggatt
     4021 taatatgggc ttaggcatct taacttattc acttgcttta gtgttaacag gtattcttgt
     4081 tggacttttc tggaaatctg gtcaaaaaca attaaaaact gttcatcctg aagcattcct
     4141 gtttaacgat catagaccaa ttaattatta cagtagttta gattcttaat attattttga
     4201 agccatgtta ttaagactta cagttatcac aataacctcg aatgaagtat cattcgaggt
     4261 tatttttcta ctaaaaatct cagtaaatat ttctatttga ttacaaatgt tacaaaaata
     4321 tttgacaacc ttctttctga aacttataat agatttgtcc ataacagagc aaaagagaga
     4381 gggatagtac atgagaaaac gttcaatttt gccaatatta gctgtcttat ttgttttcat
     4441 gagtatcttt agcgtatact ctatttttca tggtgataaa gctaaagaaa aagcaagtgt
     4501 cataaaacaa gcatctcaaa caagtcaaac ttcaaaaaaa gaggtcttac aaaagaagac
     4561 ttatccaaat ctaaataaat attcaaatct agaaattcat gtttcatcta cgaggcaaac
     4621 aatgacaata acctcgagtg acaaggtcat ctttaaaacg attgtttcaa caggagcaaa
     4681 agagagccct accccaaaag gaacttttgt catagaaccg gaacgaggag attttttcta
     4741 taacgcctca tcaaaagaag gagcctatta ttgggtctcc tttaaagagc atggtattta
     4801 tctctttcat agcgttccga ctgatcaaca aggaaatgag atcccagaag aagcaaagca
     4861 attaggaaaa gccgcctcac atggctgtgt gcgtatgtca agggcggatg caaaatggtt
     4921 ctatgaaaat attcctcaag gaacgactgt taccatcaaa tagtcttaat gtggtaaaaa
     4981 tcatgttatt aaaagcttgg agtaaacttc cagagctttt tcgtatatcc cttaaactca
     5041 attagtaaac gaaacctatc agcggaataa atgtataaag tatttcgata tcattaaaaa
     5101 aatgatagaa taatagtgtt aatcgaatat tttatcgacc tttatatgcg gttgaagaag
     5161 aataaaacta aaagattaga cctctaacaa atagatgtag acttttaagg tagtctatac
     5221 ataaggagaa tttatttcat ggctactatt caatggttcc caggacacat gtccaaagct
     5281 cgccgtcaag ttcaagaaaa tattaagcat gttgattttg ttacaatttt agtggatgca
     5341 aggttacctc tttctagtca aaatccaatg ctaaccaaaa tcgtcggaga caaacccaaa
     5401 ctaatgatcc tcaataaagc tgatctagca gatcccattc gaactaaaga atggagagat
     5461 ttttatgaat ctcaaggttt aaagacatta gctattaatt ctaaagaaca atcaacagtt
     5521 aaaaaagtaa ccgacatcgc taaaatatta atgtccgata aaatcgctaa actacgggaa
     5581 cgtggtattc aaaaagagac acttcgtacg atgattattg gaattccgaa tgcaggcaaa
     5641 tcgactctaa tgaatcgcct agcaggtaaa aaaattgcag tggttggtaa taaaccaggt
     5701 gtcacaaaag gtcagcaatg gctcaaatct aataaggaac tggaaatttt ggacactcct
     5761 ggtatcttgt ggcccaaatt tgaagatgaa ttagttggat taaagctagc attaacaggg
     5821 gcaatcaaag atcaattatt gcctatggat gaggtaacaa tatttggatt gaattacttt
     5881 aaaacctact atcctgatcg tctcaaagag cgttttaaaa gtattaattt agaggatgaa
     5941 tctcctgaaa tcatcatgac cttaacacaa aaattaggtt atcgtgacga ttacgatcgc
     6001 ttttataatt tatttgtcaa agaagttcgc gatggtaaat taggacgcta tacgttagat
     6061 atcgtcggag aacatgatgg caacaattaa agaaatcaaa gcaattttag agactattgt
     6121 tgatttaaaa gacaaacgtt ggcaagaata tcaaacagat tcacgcgcag gtgttcaaaa
     6181 agcaatcctt caacgaaaaa aaaatattca gtcacattta gacgaagaag cgcgcttaga
     6241 acaaatgttg gtctacgaaa aaaaacttta cattgaacat ataaacctta ttgcaggaat
     6301 cgatgaagta ggtagaggtc ctttagcagg accagtagta gctgctgctg ttattcttcc
     6361 accaaactgt aaaataaaac atctcaatga ctccaaaaaa attccaaaga aaaagcatca
     6421 agaaatctat caaaatattt tagatcaagc gttagcagtt ggtattggta ttcaagacag
     6481 ccaatgtatt gatgatatca acatttatga agctacaaaa catgctatga ttgatgcagt
     6541 ttctcattta tctgtagttc ctgaacatct tttaattgat gctatggttt tggatctacc
     6601 aattccacaa actaaaatta tcaaaggtga tgctaattca ctctctattg cagcagcttc
     6661 aattgtagct aaagtgacca gagataaaat aatgtctgat tatgatagca aatatcctgg
     6721 atttgcattt tctaaaaatg ctggctatgg tactaaagag catttagagg gtttgcaaaa
     6781 atacggtatt actccgatac atcgtaaaag ttttgaacct ataaagtcaa tgttgtaaat
     6841 aatacggaag aggaaaagat gaccgaaaaa gaaaaaatgt tagcaggaca atattatcgt
     6901 ccatcagcac ctgaactacg caaggataga gaagttgcac taaaaaatat gcaagccttt
     6961 aacaatgaag atgatagtag caaacgtaac gtcattttac aaaaatggtt tggtgctact
     7021 gggaagagta tccatatgga acaacgtttt gtctgcgatt atggttgtaa tatttatgtt
     7081 ggtgaaaact tctacgccaa ttttaaccaa actttcttag atgtttgtga aattcgtatt
     7141 ggcgataact gtatgtttgg cccaaactgt caactcctca ccccactcca tcctctagat
     7201 ccaatagaaa gaaattcagg attagaatac ggtgccccaa tccaaatcgg gaataatgtc
     7261 tggctaggtg gaggagtgac tattttacca ggtgttgtat taggagataa tgtcgtcgtt
     7321 ggcgctggaa gtgtcgttac gaaatcattt gaaaataatg ttgttatagc aggaaaccct
     7381 gctaagataa tcaaaaaatt agcttaatgt aagaaaaaac ctttaattat aacaaaggtt
     7441 ttttctaatt taaaaaatag ataaagagaa tattctgtta atctaatcta tcttatttat
     7501 aatgatttta aatagaaaaa ccttgatata ataatgtttt cttaagtata aaattctttg
     7561 aaaaagtaga tatttttcca aaaaattatg ttattataga tttgtgctca tttttgaaca
     7621 ctttatcttt cattttaagg agttttatga aaaagctatt aaccactcct gtaatgggtt
     7681 gtactttggt tatactttct ctaacgtccc tatttgttgg tgttaaatct atcccactgg
     7741 agcaaataac acaccttaat caatcacagg tagatatttt tttagcgagt cgcttaccaa
     7801 gaaccattag tatcctaata tctggtgcaa gtttgtcagt ctgtggcctg ctaatgcaac
     7861 aactcaccca aaacaagttc gtttcaccga caacttcagg gactatggat tgggcgaaat
     7921 taggagtagt tgttacgctg attttcttta aaaatacctc tatttttatt caactatgta
     7981 ttgcttcagg ttttgccatt ttaggatcgc tgctttttgt aactatttta aaaatgatta
     8041 cttttaagga taatattttt atacctctta tcggactgat gcttggacaa attgtagcag
     8101 cagcaacagt atttttagga acacattttc aagtactaca atctgtcaac agttggcttc
     8161 aaggtaattt ttccatcatg actagccatc gctacgaaat tctttactta gcattgccat
     8221 gtttgttttt agtgtatttt tttgctcatc agtttaccat tgttgggctt ggagaaagct
     8281 tcgctaaaaa tttagggatt gcttatgaaa agatgattta ctttggttta gttttggttt
     8341 caattatgac atccctagtc attattatcg taggagcttt acctttctta ggtttaatcg
     8401 tacctaatct aattagctta actaaagggg atcatatgtc ctcaactatc ttagagacat
     8461 cgcttttagg ggcttgtatc gtcatgatat gtgatctttt tggtcgctta gttatttttc
     8521 cttatgaagt ctcaatagga gttacgctag gagtattggg tagtgccttt ttcttgattt
     8581 caattataag aaatgaggga aaatcatgat taaggattta gagataccaa aattacttat
     8641 tttactgata ctactaattg ctaccattat tttgttttta atttatggta ttccaactga
     8701 cgcaaatgaa tttttgatta tctatatcct aaaaacacgt tatcaaaaat tgattgcatt
     8761 aattttggtt ggtatctgca ttggtagttc tagtcttatt tttcaaactc taaccaataa
     8821 ccgactttta actccgtcca ttattggttt agatagctta tatgttctta tccaaacagg
     8881 attaatgtat ttaatcggag ctcagcgtgt cataaaattt tcatctttta gcagcttcct
     8941 cctttcatta ttattaatgg ttggattcgc ttacttatta tttacgatac tttttcgaaa
     9001 taaaaaacag tctttgtatt ttgttctatt agctggactc atatttaata cattattttc
     9061 ttctatctca tcctttattc aagccattat ggaccccaac gattttatga ttttacaaaa
     9121 tcagctcttt gctagtttca atgctattaa taccaagatc ctttggattt ctttcattat
     9181 cattgtcgtt agctttgtca ttaattggcc ttttataaag gaattggatg tcttactatt
     9241 gggtaaagaa aatgcgatat cactcggcat ctcatatcaa aagcttacta ctagaatttt
     9301 tttatggtta gcattgatgg tagctatagc aacagcatta gttgggccga ttactttttt
     9361 aggcttactt gtagcacata ttacctatca cagttttcat acctttagac atcaaatatt
     9421 agttcctata gccattgtta tctgtatctt tacacttgtc ttagggcagc atctcgttca
     9481 aaatctatta cacctcactg ttcaactaag tgttctcctc aaccttatcg ggggaagtta
     9541 ttttatattc accttaatca aaggaaggaa aaacttatga ttcaaataaa caatttacat
     9601 aagttctatg gccaaaagga aatattaaaa gacattaata tttcgattcc taaaggaaaa
     9661 gtaacagcta ttttaggtcc aaatggatct ggaaagagta cattgctgag ttgtatcagt
     9721 cgacttgaac cttatgataa tggtgaaatt ttccttgata aagtaccatt agcccactac
     9781 agttcaaatg acttagccaa aacattagcc atattaagac aatctaatca cttaacactc
     9841 aagataaagg tgagtgactt gataggattc ggtagatttc cttatagtaa aggacggtta
     9901 agtcaaaaag ataaagctgt catagaatca gttatatctt atatggattt aaacgatata
     9961 gctgacgagt tcattaataa cctcagcgga ggtcaaattc aaagagcttt tatcgcaatg
    10021 acaatggcac aagatactca atatatttgc ttagatgagc cactcaacaa tttagatatg
    10081 aaatatgctg ttcaaatgat ggatcttatc aaacgatatg cgtatgagtt taataaaact
    10141 atagtgatta ttattcacga catcaatttt gcaactcact acgctgacaa tgttgtcgct
    10201 ttaaaagaag gacaagtggt gtcttgtggg actgtcgaag atgttatgca agaaaaaatc
    10261 ctttctcatt tatttgatat gcctatccgt atcgaaactg ttgatggtaa accaattatc
    10321 cttttttgta caacgtaaat aaagagattt atgtaggata acatttttag tttttatcta
    10381 ttttaggaga ttttatttaa tgacaaaaaa acttattatt gctatattag cactatgcac
    10441 tatcttaacc acttctcaag ctgttttagc taaagaaaaa tcacaaactg ttaccataaa
    10501 aaacaactat tcggtctata ttaaaaaaga aaaaagagac aagccggata ataaaaagca
    10561 aatcagcgag acacttaaag ttcctttaaa acccaaaaaa gtagttgttt ttgatatggg
    10621 agctttggat actatcacag ctttaggagc tgaaaaatct gttattggta tcccgaaggc
    10681 taaaaatgct ctaagtttat tgcccaataa cgtcaaatct gtttataaag ctaagagata
    10741 ccaagacgta ggaagtctct tcgaaccaaa ctttgaagct attgctcgta tgcaacctga
    10801 tgtggttttc ctaggagcac gtatggcttc tgttgataat attgaaaaat taaaggaggc
    10861 tgcacctaaa gcagcattag tatatgctgg agtcgactca aaaaaagtat ttgacaaagg
    10921 agttgctgag cgtgtcacaa tgttagggaa aatcttcgac caaaataaaa aggcaaaaac
    10981 ctttaataaa gatatcgcac aagctgttct taaattgcag aaaactattg agaaaaaagg
    11041 taaacctaca gctctatttg taatggcaaa cagcggtgaa cttttaactc aatcaccttc
    11101 tggtcgtttt ggttggattt tctctgtagg tggatttaaa gcagtcaatg aaaatgaaaa
    11161 actaagttca catggtactc ccgtatctta tgaatacatc gctgaaaaaa atcctaacta
    11221 tctctttgtt ttagatcgtg gagcgactat tggacaagga gcttcatcaa aagaactttt
    11281 taataacgat gttattaaag caactgatgc tgtcaaaaac aaacgtgttc atgaggtaga
    11341 tggaaaagat tggtatatca attcaggcgg aagccgagta acactccgta tgattaaaga
    11401 tgtacagaac tttgttgata atcgttaatt ttttgtcaaa ggctaggaaa aatcctagtc
    11461 ttattttttt gaaaaaatag ctattatcaa tgcttcagag tatagattga tcttatcact
    11521 ttattttgtc tattattttc gaatatagtc aatggaggta accttatgaa tcattttgaa
    11581 ctatttaagc ttaaaaaagc tggactaaca aatcttaata tcaacaatat tatcaactat
    11641 ctcaaaaaga atagtttaac ttctctatct gttcgcaata tggccgtagt atcaaaatgt
    11701 aaaaatccta ctttctttat agaaaattat aaacagctag accttaaaaa acttcgacaa
    11761 gaattcaaaa aatttccagt tctatcgatt ttggattcta actatccttt agagttaaaa
    11821 gaaatatata atccaccagt tctacttttt tatcagggta atattgaact tctatctaaa
    11881 cctaaattag ctgtagtggg ggcaagacag gcatctcaga taggttgtca gtctgttaaa
    11941 aagattatca aggagactaa caatcaattt gttatcgtaa gtggtttagc gcgtggcatt
    12001 gatacagcag cacatgttag tgctttaaaa aatggcggca gtagtatagc tgttatcggg
    12061 agtggtttag atgtttatta tccaacggag aataagaaac ttcaagaata tatgtcatat
    12121 aatcatctcg tattatcaga atattttacc ggagaacaac ccttgaaatt tcatttcccc
    12181 gaacgtaacc gtattattgc agggctgtgt caaggtattg tggttgccga agctaagatg
    12241 agatctggaa gtttaattac ctgtgaaaga gcattagaag agggacgaga agtttttgcc
    12301 attcccggaa atattatcga tggcaaatca gatggatgcc accatcttat ccaagaagga
    12361 gctaaatgca ttatttcagg aaaagatatc ctttctgaat atcagtagtg ttttgctttc
    12421 tactatagga gaagttttct attgacagtt taagaaaagt gagttattat gctataagtt
    12481 tacgatatag aaagtgatac ctatggcaac tacgacaaaa acgtcgacca aaaaaacaag
    12541 taaaaaaaaa tcagctactg ctaaaaaaaa tctagttatt gtggagtctc ctgcaaaagc
    12601 aaagactatc gaaaaatact taggacgtaa ctataaagtt gtagcctcag taggtcatat
    12661 ccgtgattta aaaaagtcaa gtatgtccat tgattttgag aataattacg aaccacaata
    12721 tattaatatt cgtggtaaag gacctctcat taatgattta aaaaaagaag ctaaaaaggc
    12781 taaaaaagtt tacctcgcga gtgacccgga ccgtgaagga gaagctattt cctggcattt
    12841 agcgcatatt ttagatttag acaaagaaga cagaaaccgt gttgttttca atgaaatcac
    12901 aaaagatgct gttaaaaatg cttttgtgga acctcgtcag attaacatgg atctcgttga
    12961 tgctcagcaa gcacgacgtg ttttagatcg cattgttggt tattctatct cacctatttt
    13021 atggaaaaaa gtaaaaaaag ggctatcagc tggacgtgta caatcagttg ctcttaaact
    13081 aatcattgat cgtgaaaatg agattaaggc tttccaaccg gaggagtatt ggactattga
    13141 cggttccttt aaaaaaggaa cacgtaaatt caatgccact ttctacggtt tagacggaaa
    13201 gaaattcaaa ttatcgaata atgaagacgt caaaacagtt ctaaaacgta ttaaaactga
    13261 tgaattctta gttgaaaaag ttgaaaaaaa agagcgtcgt cgtaatgcac cattaccgta
    13321 tacaacttct tcattgcaac aagatgcagc taataaaatc aattttcgaa ctcgtaaaac
    13381 catgatgatt gcgcaacagc tgtacgaagg acttagctta ggcacagcag gtcatcaagg
    13441 tctgattacc tatatgcgta ctgattctac acgtattagt ccgttagcac aaaatgaagc
    13501 aacagaattt attactaacc gttttggtgc aaattattct aaacatggga ataaagttaa
    13561 aaatgcttct ggagcacaag acgctcacga agctattcgc ccatctagtg ttaatcatac
    13621 acccgaaagt attgctaagt atttagacaa agatcaacta aaactttaca ctcttatctg
    13681 gaaccgtttt attgcaagcc aaatgacagc agcagtcttt gacacaatga aagttaattt
    13741 aacgcaaaat ggtgttactt ttattgctaa tggtagccaa gtcaagtttg atggttacat
    13801 ggctgtttat aacgatactg acaaaaataa aatgttacca gatatggagg aaggagaaag
    13861 tgttaaaaag gttaatacga atcctgaaca acacttcact caacctcctg caaggttttc
    13921 agaagcaagt ctcattaaaa cacttgaaga aaacggtgta ggtcgtcctt caacttatgc
    13981 cccaacgctt gagactattc aaaaacgtta ttatgttaag cttgcagcta aacgcttcga
    14041 accaactgaa cttggtgaaa ttgttaatag ccttattgta gaatttttcc cagatattgt
    14101 tgatgttacc ttcactgccg aaatggaagg gaaactagac gaagttgaga ttggtaaaga
    14161 gcagtggcaa aagattatcg atgaatttta taaaccattt gaaaaagaac ttgcaaaagc
    14221 agaaactgaa atggagaaaa ttcaaataaa ggatgaacct gctggatttg attgcgagct
    14281 atgtggatca ccaatggtaa taaaactagg acgttatgga aagttttatg cgtgtagcaa
    14341 tttccctgaa tgtcataaca caaaagctat cactaaggaa ataggtgtta tttgtcctat
    14401 ctgtcaaaaa ggacaagtta ttgagcgaaa aacaaaacgc aatcgtatct tttatggctg
    14461 tgaccgctac ccagaatgtg agtttacatc ttgggacaaa cctattggac gaacttgccc
    14521 caaatcaaat gatttcttag tggagaaaaa agtacgcggt ggtggtaaac aagttgtttg
    14581 ctcaaatgaa aaatgcgact accaagaaga gaaaattaaa taaagtggaa tctgagtgtt
    14641 tagctcagat ttttaaaaaa attattattt tacataactt aatttatgta tttattttta
    14701 tgggagaatt aataaaaaag tgctataata taggtgtatt catagtgtga ggtgccgtta
    14761 tgtcctatat tgaaatgaag cattcctata agcgttatca aacaggtgaa accgaaattg
    14821 ttgctaacaa tgatattagt ttcagtattg aaagaggtga gttggtggtt attcttggtg
    14881 catccggcgc tggcaaatct actgttctca atatactagg agggatggat agtaatagtg
    14941 aaggtgaagt tttaattgat ggtaagaata ttgctaatta taccatcagg gaattgactc
    15001 gttaccgtag atatgatgtc gggtttgttt ttcaattcta taacttagtc cctaatctaa
    15061 cagcactaga gaatgtagaa ttggcttcag aaattgttcc aaaggcatta gatgcccaac
    15121 aggcattaga aaacgtaggt ttgggacacc gtatcaatca ttttccagca cagctttccg
    15181 gtggcgaaca gcaacgcgtt gcaatagcac gagctattgc caaaaaacct aaactgttac
    15241 tttgtgatga acctactggt gctttagatt accaaactgg aaaacaagta ctagccatcc
    15301 tgcaaaagat ggcgcaatca aaagaaacaa ctgtgatcat tgttactcat aatactgcct
    15361 tagcgccaat tgcaaatcgc gtgatccata tgcatgattc aaaaatcagt gatattgtaa
    15421 taaacgagaa tccaagtgat attcagaaca tagaatatta gaggttatac agatgggaaa
    15481 gacattttgg aaagatatct atcgatcaat tacgacttca aaaggtagat tttcatcaat
    15541 cctcttactt atgatgttag gctcatttgc ttttatagga ctgaaggtct cagcacctaa
    15601 tatgcaaaga acagcacaaa attaccttgc tcatcatcat gttatggata ttaccgtttt
    15661 caattcttgg ggacttgaca aacatgatca aactgtttta gaaagcttaa aaggatcaca
    15721 agttgaattt tcttactttg tagatacaac ccctcaacag aatagtaaat cttatcgatt
    15781 gtattccaat actaaaacga tatcaacctt tgatcttgtt aagggacgtc tccctttaaa
    15841 taaatcagaa attgctctct catttcagga acgaaaaaaa tatgcaatag gtgataaaat
    15901 taattttaaa caagataaaa acaaactgtt ttcaaataca ggtcctctaa ctattgtagg
    15961 ttttgttaat tctaccgaga tttggtctaa gacaaacctt ggtagttctc aaacagggga
    16021 cggcgactta gatagttatg gcgtattaga taagacagca tttcactctc cagtatatac
    16081 aatggcaagg gtaactttta aagatcttag gttgattaac cctttctcaa taagttacaa
    16141 agaaaaagta gcgaaatacc aagaaaaggt atcgcgaaaa ttaaatattc acaataaaat
    16201 aagatatacc aaaacaaaaa aagagagcct acgtaaaatc gatgaggagg aaaaaagcct
    16261 actaaaagct caaaaacaaa tcaatcggtt agataatgat agcctagcga tgccactatc
    16321 tcaaagacaa gctattcaaa tgaaaattaa acaagaccga ctatctcttt tgaaacgaac
    16381 aaaagagctt ctaaaattga gacataacac ccaaataatg gaatcgcctc aaattattgt
    16441 ctacaaccgt actacctttc ctggaggaca aggatataac acttttgact ccagtacaaa
    16501 tagcacttct aaaatcagta atcttttccc cattatttta tatttagtcg cagcattagt
    16561 aaccttaaca acaatgacta ggttcgtcga ggaagaaaga accaacgcag gcatattaaa
    16621 agcactaggc tatagtgacc gccaagttat ctttaaattt attatttatg gttttatagc
    16681 aggaacatta gggactactt tgggtatcat tggagggcat tatcttttac ctcgtattat
    16741 ttctgatatc atttccaaag acctgactat tccaaacaca cagtatcatc ttttcttaaa
    16801 ttacagttta ctagcctttg ttttttcatt attaagcatt gttcttccag tttttgttat
    16861 cactcgacgt gagttgaaag aaaaagcagc atttttatta ttaccaaaac caccagctaa
    16921 aggatctaaa atagctttag agtatatcaa ttggatttgg aaaaaactgt ccttcacaca
    16981 aaaagtaact gctagaaata tttttcgtta caaacaaagg atgattatga ccatatttgg
    17041 agttgctgga tcagtagcgc tcttattttc aggtctagga atacaatcct ccttaaagca
    17101 aaccgtcaat gaacatttcg gtcgtattat gccctatgat atattgctaa catataacac
    17161 aaatgcttct cccccaaaaa tacttgaact actttcaaaa gattcaaaaa tagacaaata
    17221 ccaaccaata caccttgaaa atcttgacga atccattcct ggacaaatta ataaacagtc
    17281 aatttcccta ttcataacag acaaaaaaca attactacct tttatttatt tacaagaagc
    17341 aacaactaat aagtcattgc acttgaatag taaagggatt attatttcta aaaaattagc
    17401 tcaattttat catgttaata ccggtgattt tatccattta tctcattcac aaacacttcc
    17461 ttctagaaaa ttaaaaataa caggagttgt caatgcgaat gttggtcact atatttttat
    17521 gacaaaacaa taccatcgaa ctatttttaa gaaagaagct aaagataacg cttttttggt
    17581 taagttaacc aaacataaaa tcgcaaataa cttagcagaa aaacttttag aaattaatgg
    17641 agttgagtct cttacgcaaa atgctcttca gctggctagt gtagaagccg ttgtgcgttc
    17701 tttagatggc tctatgacta ttttagttgt cgtatcttta ttgttagcca ttgtaattct
    17761 ctataatctc actaatatta atttagcaga acgta
//
LOCUS       DATRNX010000036        17711 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRNX010000036 DATRNX010000000
VERSION     DATRNX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(18..947)
                     /locus_tag="VBC81_000984"
     CDS             complement(18..947)
                     /locus_tag="VBC81_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912034.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBC81_000985"
     CDS             complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBC81_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6912035.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            complement(1642..2740)
                     /gene="prfB"
                     /locus_tag="VBC81_000986"
     CDS             complement(join(1642..2667,2669..2740))
                     /gene="prfB"
                     /locus_tag="VBC81_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6912036.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBC81_000987"
     CDS             complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBC81_000987"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6912037.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            complement(4023..5954)
                     /locus_tag="VBC81_000988"
     CDS             complement(4023..5954)
                     /locus_tag="VBC81_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6912038.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            6106..6966
                     /locus_tag="VBC81_000989"
                     /pseudo
     CDS             6106..6966
                     /locus_tag="VBC81_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(7011..9695)
                     /locus_tag="VBC81_000990"
     CDS             complement(7011..9695)
                     /locus_tag="VBC81_000990"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6912039.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(10039..10437)
                     /locus_tag="VBC81_000991"
     CDS             complement(10039..10437)
                     /locus_tag="VBC81_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6912040.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            10501..11817
                     /locus_tag="VBC81_000992"
     CDS             10501..11817
                     /locus_tag="VBC81_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6912041.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            11889..12701
                     /gene="yidA"
                     /locus_tag="VBC81_000993"
     CDS             11889..12701
                     /gene="yidA"
                     /locus_tag="VBC81_000993"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6912042.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            12686..13921
                     /locus_tag="VBC81_000994"
     CDS             12686..13921
                     /locus_tag="VBC81_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6912043.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIIDYSNKELVGFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQRDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNGIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            13934..15145
                     /locus_tag="VBC81_000995"
     CDS             13934..15145
                     /locus_tag="VBC81_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6912044.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            15164..16399
                     /locus_tag="VBC81_000996"
     CDS             15164..16399
                     /locus_tag="VBC81_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6912045.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            16587..17519
                     /locus_tag="VBC81_000997"
     CDS             16587..17519
                     /locus_tag="VBC81_000997"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6912046.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIFHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
BASE COUNT         5811 a         3441 c         2835 g         5624 t
ORIGIN      
        1 gatttcatct ctttttatta aatcttaagg taacgtctca ttgataggac agatccaagt
       61 gagccaataa taataccaat aatcaccata ccaccaataa ttgctggaac cataatatcc
      121 ataggataca atgaaacatg gcttgtctca aattttggat taaacttgtt aaacacaaat
      181 tgataaccaa aatagaaaat cagcgatgga acgatagccc ctaaaatccc tacccaagca
      241 ccttcaaaaa agaacggtcc tcgaatataa gaatttttag cgccaactaa tctcatgatt
      301 tcaatatctg tacggcgtga cataatcgac atacgaatag tatttgaaat caaaaagata
      361 gcaaggacaa ttaataaggc aacaccacca aatccccata tacggatgtt atctgtgaga
      421 ttagatagtt ttgttgaatc aataggttct ttagtataat caacaccttc aatgcgtcca
      481 attgcttcac tgacactctt agaaactttt gcagaacttg tcccaactac gtaaacatct
      541 aacaaagcat ctttgtaggt atcatcaata acatctgaac catactcttt ttgaacttct
      601 tttaattgtt cagctttact tgaataagtg actttctcta caccagaaat acgtttaatc
      661 ttgtcataaa ctttatgata atcaggatta tcttttagct ttccgtcctt atctttgact
      721 tgtttttgag catccgttga gtcaacattt aaaaaggcac tgatagtaaa attacctgaa
      781 acatcagttg ttaacttttc aacattcagt aaaactgatg aaaacaagcc tacaagaagt
      841 aatgttatgg taactgatgt tacagaagca aaagtcatcc aaaagttacg ttttaagttt
      901 ttaagcgatt cccagaaatg tctaaagaaa ttaatcatga taaccatatt ctcccttctc
      961 ttcatctcgg ataacgctac ctgcttcaat ctcaatgaca cgatgacgaa gtgtgttaac
     1021 aatttgacta ttgtgtgttg ccatcaaaac agtcgttcct tgaaggttaa tacgttccaa
     1081 tagatgcatg atttcccaag cgatttctgg atccaaatta ccagtaggtt catctgcaat
     1141 taataatttt ggattattaa caatcgcacg tgcaattgcc acacgttgtt gttctccacc
     1201 agataactgt gttggaaaag aacgcatctt gtgttttaaa cctactaatt ctaaaacttc
     1261 tggcacgcgt tttttaatat gacggcgctt agctccaata acttgcatcg caaacgcaac
     1321 attctcataa actgtttttg ttggtagtaa tttataatct tggaagacaa ctccaatact
     1381 acgacgtaaa ataggaattt gacgacgttt gagtttattg agattgaatt cccctacctt
     1441 taatctacct gaacttaatt tttcttcacg gtataggagg cgaatcaagc tagatttacc
     1501 agcacctgac ggaccaacta aatacacaaa ctcaccttgc tgaattgata aatttaagtt
     1561 tctcaaggca gttgttgaac ggcgatactt tttagtaaca ccagacattt ctattagtgc
     1621 catgtttctc cttcattatt tttagtcttc aatgcgccac ttaaggtagg catcaataaa
     1681 gccattgatt tctccatcca taaccttatc tacctgagca agctcaaagt tagtacgatg
     1741 atccttcacc atcgtataag gggtgaaaac ataagaacga atctgacttc cccaagtgat
     1801 ttctttttta tcacccttga gagcatctac ttcttgtgct ttcttttctt gttctaactg
     1861 atatagctta gcctgcaaca tcttcatagc gcgatcgcgg ttcccatatt gagtacggtc
     1921 aacggtagac gataccacaa ttccagttgg aatatgcgtc aaacgaacac ctgttgatac
     1981 tttattgacg ttctgaccac cagctcctcc ggaacggaaa gtatccattt taatgtcatc
     2041 atcacgtact tctacttcaa tcgtatcatc taactctggc atcacttcaa ctgaagcaaa
     2101 tgaggtatga cgacgtttag ccgaatcaaa aggtgatata cgaacgaggc gatgaactcc
     2161 catttctgat ttcaacaaac cataagcatt gggaccttca aaagagaggg taaccgattt
     2221 aataccagct tcatcccctg cttgataatc caatacttct actttgaaac cattagcatt
     2281 gccaaaacgg gtatacattc gtaagagtaa atctccccaa tcttgcgctt cagttcctcc
     2341 tgaaccagga tggatttcta agatagcatt attgtggtca taaggctcag ataagagaag
     2401 cgtcatttca taagcaccca ttatcttatc tagctgcatt agattttctt ctaattcttc
     2461 tttcaaagaa tcatcttcgt caagcatctc aagtaagagt tctgtttcgt cagataattc
     2521 ttgcatatta tggaaagtat catacttacc ttttaactca ttcagttctt gagatgtctt
     2581 ttgcgctgca atattgtcat tccaaaagtc tggctcagtc atttgatttt caagaagagc
     2641 aatctcctct tccaaacgat ctaagtcaaa gagacctcct gaagctagtc aacttctctt
     2701 tattttctac tattttttgg cgaatttcag ccacttccat gagatttctc caatctttgt
     2761 taatctgttt tattttatca taaggttctt aaaaatacca taaaaggctt aaaattgcca
     2821 tttatgacgg attaattcca actcttttcg gctatcttca tcttttaagg ttaaattact
     2881 cataaactcc agtatctcat cgtttggttt tttgacaatt tctcccaatg cccatatagc
     2941 tgttgcagta tgaatagggt tattattttt atcaataatc tctattaatt taaccactgc
     3001 ggttttatca tgagcatttg ctagcgcaat aatggcatta cgctgtaaaa tatttttacc
     3061 tcgccaactt ccagcaatca taccaaattt ttctttaaat tgtccatttg ataaactcaa
     3121 aaatggtatt aattctggtt gagccaactc aggatcaatc tctgtcgcca atggattatt
     3181 tatacctttg ttataaggac agcaaatttg gcaaatatcg caaccataga taactgtttt
     3241 aatctttttt cgaaactcta tgtccatcat tcccttatct tgggtttgaa atgatagaca
     3301 acgcttggca ttcattgagc catcaccaat taaacaagaa gttggacagg catctagaca
     3361 acgacgacag tcaccacaat cataatcgac aggcttgtct ggctcgatct ctaaattggt
     3421 aattaattct cccaaaaaca tataagagcc atattcttta gaaataacta gaccattttt
     3481 tcctataaaa ccaatacctg ctctttgggc aactgcagta tcaactaaag ctccagtatc
     3541 gaccattgct ttttgttgca aaggaaaatc tcgacagagc tcttcaatgc cttttgatag
     3601 tctgtctaac ttttcgccga ccacataatg ataatcaagc ccccacgaat taggtgtaat
     3661 ttttcctcgt ttataaggtg ttttttgagg ttgctgagga agtttgtgag gataagcaac
     3721 gccaattgaa ataatcgttt tagccgattc taaaagcctt tcagggtaga tacgatcctc
     3781 tatgacttta tgttcaaatc ctgaattgcg cccctcttcc acagaagcac gcaatgactt
     3841 ttccaaataa tcaaagttat ctgccgtcgt aaaaccaatt ttagagatac caatttcttt
     3901 tgctaatttt tgaatttctg cctttatgtc cataatatta ttttaagtca atttaataaa
     3961 agagacaagg taagaaactg aaaaattaaa tcctacatat caattagtaa attacttaaa
     4021 cactagtcat catattcctg aaaatgacga taaaggtgat ctaagtcttt aatctctttg
     4081 gttaactttt gtccaatagt tgtatctttt acaagaatcc tatccttggc ttcttctact
     4141 aatcgcttcg tatcaatgat atagttggtt ccacttaaaa cttcctcaat ggatgtaaaa
     4201 ggaaggtgtg aaattaattg tatcccatag ctattataaa tcaaagtgta gccagctaaa
     4261 cctgtatttt tctgataacc tttggcaaag ccaccatcaa ttactaacat cttaccattt
     4321 gcttttatcg gttgctcacc atttttttct ttgactggcg tatgaccatt gacaatatgt
     4381 ccaaacttag gcaaatcaaa ctcttgtaaa agggcagtgc aaatatcatc tttatctctc
     4441 agtttaaagt agggattttt tctttcttga tgagttactt tgtcggaaac ataatagcgc
     4501 tcaaaagtag tcatggcatt cttaccaaaa agagaagaat tttcaccaca ccaaagatac
     4561 caaacgatat ctgtcgccaa atcatcatgt ttctctggtc tagcatatgc tagacgtatt
     4621 tgactttcaa ataaatccag taaatcccta ccaccataag tttttcctgc aattttaaag
     4681 cttttaaaat caccattact atgcattggt atgcaaccat ggaataaaag attatcatta
     4741 taggttaaat acatacttcc tttctcaaat aggaaatcaa tatgtttctt taaactttca
     4801 gaagtctgga aggctatcat caattgttgt aaaagctctt cttcctcact tgttaatcga
     4861 ctgggatttt tcgaatcaat gcatctacta ttaaaatctt taagttgatg cacaactccc
     4921 tttatagata tacttaaatc tttataattt acctggttaa taagttgtcg attatgcatt
     4981 tgaaattctg gacgcctgtc aatcaattgg gcttccaatt taaactgtaa aattgctgtt
     5041 gcttgttgta tcatatttaa caaatctaat tcatctggat gagtcatttc ctctccatct
     5101 aatatggggg cgaaagaagg tagaggttca tagtaacggc gtgagtaatc aaccaagcga
     5161 cgcaaattga taccatatcg gtcttcaatt aaagtaatat tattataacg tgcagcaata
     5221 cggataacat ttaccataca aaggtaggaa ccagatgctg ctcccatcca agtgacatca
     5281 tggttccccc attggatatc caaatttgga aaagccatta acctattcaa gatacgatca
     5341 ggatacctac cacgatcgta aatatcccct acaacatgta aatggtcaat gattaaaact
     5401 tgaattgttt gggataatac tataattaaa tcttctactt ggtctaattc aaataatttt
     5461 tcaataatag tatcaaaata gcttttcttc tctgggttac ggtcaatctc agctaaaagt
     5521 tcttcgataa tgtaagcaaa cttacctggc aattgcttac gtactttaga ttttgtatat
     5581 ttactagaaa aatattttaa aacttgtatt tgtaacggaa tcatttccca taacttcgtt
     5641 ttataagcac ttactgacaa gttcatttta tcgaaggcta atttttcctt tgggtaataa
     5701 agcagtatac aaaaatcatc taaatcaaca gcaactattt tctgccaatc aaagcagtct
     5761 aataatttag ctctaataga ccctgcacct gtccgtaaga gataatcaac agcatcgtat
     5821 tccccgtgta aatcactaat aaaatattcc gtccctttgg gtaattgaca aatagcttct
     5881 aaattgatca tttctgttac gatatcttct tttcgaggaa atttttcttt caagagctta
     5941 taaaaattag acatgagacc tccttaaggc accttaaata taaagcttct aaacacaatt
     6001 atgaaagcgc attctacaat gacatatcta ttatagaatg actttaaaac tatttcaaaa
     6061 atttactgtt tatctctttg aactacaata tggtacaata cccttatgac caaacttgct
     6121 atcatgagtg atttacatat tgacctcaat catttttcta cctttgagac gaaaacgtta
     6181 attaatttgc ttaagaaaga aaaaattgat cacctccata tcgctgggga tatatctaat
     6241 cattttacta aagatacact tccctttatc aataacttaa aaaagcatat taaacttagc
     6301 tataatttag gaaaccatga tatgcttgat ttaacagaaa cagaaatcca acgccttgac
     6361 tttcaaacct atcgatttga caaaaaaatg ctactagctt ttcatggcta gtatgattac
     6421 agcttttcaa ataatagaga tatcaaggat gttgaaaaat taaaaaagac tttttggttt
     6481 gatagacgtt taaaacgtcc aaataatgat gtaactatcc aagcatcaat tttaaaacgc
     6541 ttagacgaaa tattagcaaa agtcgattct tcaaatatca ttattgctat gcactttgtt
     6601 ccccataaac agttcacgat gactcatcct agattctctc cttttaatgc ttttttgggg
     6661 agtcaggctt atcatgattt attccaaaaa tatcatatca aagatgttgt ttttggtcat
     6721 gcacaccgct catttggcga cgttaaaatt ggagagacta cttatcattc tagaccactt
     6781 ggatatatta gagaatggaa tttaactatt gactttgtca atcagaatcc caatcataac
     6841 cctaatttaa cctggaatct tagcaaaagg cataatgccg ttaagcattt ggatagtttt
     6901 gaaaactatc caaaaaaata ttttgaagac gaattacgca actctatgac aatatttgat
     6961 tgctaaaaaa ctcacttaga tattctaagt gagttttttt atcagctaaa ctaaatagct
     7021 ttttcgtctt gtcctaattt acgttggata aatttaacaa tttccgttaa cactaccatc
     7081 aataaacttc cgataactac agttaaccat tgtgttgaac ttaaatgagt tacatggaaa
     7141 agtttattaa atccaggaac aacaatagtc accatcaaaa gaatgaaagc tactggaata
     7201 gaccagttaa aggttctatt tttaaaagct ccaactgtaa agattgattg gtaaactgat
     7261 ttaacgttaa atgcatgtac caattggatt aaaccaagcg ttgcaaaggc catcgtaagg
     7321 gcatctgcgt gaatcatgcg ataaccagca tgttctggat acatcaatgc ccaaccatag
     7381 acaccaagta ctaagatagt ttgtaaaata ccttgataga taatagctcc cataacacca
     7441 ccatcaaaga agtttgactg gcgaccacga ggtttatgtg tcataactcc tggttcagct
     7501 ggctcaactc caagagcgat agctggaaga gtatctgtta ccaagttaat ccaaagaagg
     7561 tgaaccggtg caagtacatc ccatcctaat aaagttgcaa agaaaattgt aaatacctct
     7621 gccatgttgg ctgaaagaag atattgaata gatttttgga tatttgagaa gactttacgt
     7681 ccttcctcaa cagccacaat aatagttgca aaattatcat cagcaagaac catatcagaa
     7741 gcacctttgg aaacttctgt accagttatt cccataccaa taccaatatc tgctgttttt
     7801 agcgatggag catcattaac accatcacca gtcatggcaa cgactttgcc atcgttttgc
     7861 caagctttca cgatacgcac tttatgttca ggagaaacac gtgcatagac agaatattgt
     7921 ttaaatactt tttgaaactc ttcgtcactt aattcattaa gttcagcacc cgtaaagaca
     7981 tgatcttctg tgtcatttgc atcaataata cctagacgct ttgcaatagc ttctgcagta
     8041 tcttgatggt ccccggtaat cataatcgga cgaattcctg cttctttagc aactcgaaca
     8101 gcttctgccg cttctggacg ctcagggtca atcattccca ctaaacctga gaatactaaa
     8161 tctgactcaa caatatcagt ctctaaagtt ggaatttgtg tttcatattt ataagccatc
     8221 atcaagacac gaagagcttg ttttgcgagt tctttattgg tatttaggat agcctcttta
     8281 tcttcagccg tgatatcacg aaccaagcca ttatcttcaa ttttagtaac tcgttttaat
     8341 aattgatcag gagcaccctt gactgcgata aagtagcgac catctgattc tttatgaatc
     8401 gtagacatta acttacgatc tgaatcaaat ggtaactcag cgacacgtgg ttcatttttc
     8461 aaaacttcac ggacatcaaa attcttatct aaaccaaatt gtaccagtgc agtctctgtt
     8521 gggtcaccga ttaattttcc tgaaggatca atcttagtat cgttggagaa attcataata
     8581 cgtaaagtgt tattatcaac tgaaatttct tccgaagatg attggagaac gccattagta
     8641 tacacttttt cgacagtcat ttgattcatt gtcagagtac cagtcttatc cgatgcaatg
     8701 atttctgtag aacctaaagt ttcaactgca gggagtttac gaacgataga attacgttta
     8761 gcaagagttt ttgttcccat tgataagaca atcgtaacaa ttgcaggaag cccttctgga
     8821 atagctgcaa ctgctaaagc aactgaagtc atcaatcctt caatccaacc ttctttacgg
     8881 acaaatatcc ccactaagaa agtaataacc gcaataatca cgattaagta agtcaataat
     8941 ttagaaagtt gaactaaact ttgtttcaat ggtgtttcag attcatcagc atttgccaac
     9001 atatcagcaa ttttaccaac ttcagtatac atccctgtgt tagtaacaac accatatcct
     9061 cgaccatacg tgacatttga attttgatac gccatattca cacgatcacc aatacctgca
     9121 tcctctgcta ctacttgaga tatgtctttc tcaacaggta ctgactcacc agttagagct
     9181 gcttcttcaa tttttaaaga cgcggcttct aaaagtcgta aatctgcggg aacaacatct
     9241 cctgcttcta acataacgag gtcaccagga actagttctt tagaatccac ctcaatagta
     9301 tgaccatcac gacgaacacg cgcaattggg cttgacatat ctttgagggc ttcaatagct
     9361 gcttcggctt gcccctcttg ataaacacca aaagctgcat ttaagataac aactgccaag
     9421 ataatcaatg catccgttaa gccatgcata ccttccgtaa taacagagag agctgcagca
     9481 accagtaaga taataatcat taaatctttg aattggtcaa aaaacttagc tattagacta
     9541 cgttttttcc cttcttcaag ctcattgcga ccatacatct ctaagcgatt tttcgcttct
     9601 gttgttgata atccttcacg agaagattct aaagacgtta gaacctcttc ttgaccttgt
     9661 gtgtaaaaca aggatttttt ctgttctttt aacaaaacct ttcctccttt ggtctctcca
     9721 caaacaaaaa gagacctgtt tttatgaaaa caagtctcgc tgtttaagac aacgccggaa
     9781 atttctttcg taatgacgac attgccacgg taaaccgtct gctactccct tgatatgtta
     9841 atcattgtaa cagaaaaaaa tccatttttc aattaattat ccttgctatc actaataatc
     9901 tcaagggaaa aaacgaagtt catctagtcg aaataaacca tactgcagtt tatcaaataa
     9961 atggctctca taaagagatc ccagcgtctt cagtagacga aattcataat gactactctt
    10021 ttgttaatgg taattatctc aataccattg aatctttaat tcataatttg caaaacaggc
    10081 tcctgtctcc gtttgcttaa ggacataatt gatgtgcaac ctttgctgtg acaaatcaaa
    10141 ctgatagtga cttgtctctg taatcaaatg ttgaatacca agtggagtgg ggatattaac
    10201 caaagcaggc gtttcacgat gaaaacgcat tacagactta ggatttgaaa acctcgtcat
    10261 aagcaattcc tcatgatttg ccttaataac tactctctcg ccctcagcat taatataatt
    10321 caaataaacg taacttcctt tttcaataaa ttgacaatcg tgaatctcat gaatcatttc
    10381 ggtttcgttg tcaatttgaa tgttattgtt aatttctagt ttcatgactt ctctcacttc
    10441 ttttatcctt ctattctacc aatttctgac tcaagtttca atttttgcta taatatatat
    10501 atgaatgaaa aagtatttcg tgaccccgtt cacacttata tccatgtaaa taatcaagtc
    10561 atatatgact tgattaacac taaagaattt caacgtttac gacgcattaa acaaacctca
    10621 acgacttctt ttaccttcca tggtgcagag catagtcgtt tttcacattg tctcggagtt
    10681 tatgaactgg ctcgtaaagt tacagaaatc tttgatgaac attattctga tctttggaat
    10741 aaaaatgagt ccctgctaac gatggctgcc gccctactac atgatatcgg acatggcgca
    10801 tattctcata catttgagcg tctcttcaat actgatcatg aagcttacac tcaggaaatc
    10861 atcaccaatc ctaccacaga gattaatgct attttacgaa aggtggctcc tgattttcct
    10921 gataaagtag ctagtgttat taatcatagc taccccaata aacaagttgt tcaattaatt
    10981 tctagtcaaa ttgattgtga tcgaatggac tatcttctca gagattctta ctacactgct
    11041 gctagctatg gacaatttga tttaacacga attttacgcg ttattagacc aaccgattct
    11101 ggaattgctt ttgctcgtaa cgggatgcac gctgtggaag attatattgt tagtcgcttc
    11161 caaatgtata tgcaagttta tttccatcca gccagtcgtg ctatggaatt actcttacaa
    11221 aatttactaa aacgtgctcg cttcttattt gatacccatc gcgatttttt tgaacaaact
    11281 tctccaaatc ttattccttt cttcacggat caatatgatt tacaagatta tttagcccta
    11341 gatgatgggg taatgaatac ttacttccaa tcttggatgc aagctgatga taacattttg
    11401 gcagatttag ctaatcgttt tatcaatcga aaagtcttta aatcaattac ctttgaagag
    11461 gctgataaag aaaatctagt taaaatgaaa gaactagtct cacaggttgg ttttgatccc
    11521 gattattaca ctggtgtcca tgctaatttt gacttacctt acgatgttta tcggcctgaa
    11581 cattcaaatc cacgaacaga aattcaaatt atccaaaaga atggacaact tgctgaatta
    11641 tcaagcttat caccgattgt aaaagcatta actggatcta attatggtga tcaacgtttt
    11701 tattttccaa aagaaatgtt gacattagat agcctatttt caagtacaaa agaagaattt
    11761 caatcttata tcacaaatga gcatttgaca ctcacgaaag ataatagcca ttcatgacaa
    11821 accctttatt ttttagtaag atataaggtg agtattttag gatattgaaa taaagagtgg
    11881 gagataaaat gacttcaatt aaacttgttg ccgtagatat tgacggcacc ctattaaata
    11941 gtaagcgtga aattacacct gaagtggcta aagctgttca agaagcaaaa tcaaaagacg
    12001 ttaaaattgt tattgctaca ggacgtccaa tcatcggagt ccaagatctt ttagaagaat
    12061 tgaaactcaa tgaagaggga gattatgtta tcacctttaa cggcggtcta gttcaagaca
    12121 cagccacagg agatgacatc atcaaagaaa ccttaaccta tgaagactat ctggatttcg
    12181 aactgttagc gcgtaaatta ggcgtccaca tgcacgccat tacaaaagaa ggaatttaca
    12241 cagcgaatcg tgatattggt aaatatacaa tacacgaagc aactttagtt aatatgccgc
    12301 tcttctatcg tactcctgaa gaaatgggag acaaagaaat cattaaacta atgatgattg
    12361 atcaacctga catcttagat gcagcaattg ctaaaattcc aaaaaaagtt ctcgataact
    12421 acaccattgt aaaatcaact cctttttatt tagaaatttt acccaaaaac gttaataaag
    12481 gcactgcact tctccacctt gcagagaaaa tgggattaac tgtagatcaa acaatggcta
    12541 ttggtgatga agaaaatgac cgtgctatgc ttgaagttgt tggaaatcct gttgttatgc
    12601 aaaacggcaa tcctgaacta aaaaaaatag caaaatatat caccaaatca aacgaggaaa
    12661 gtggcgtagc ctacgcacta cgtgaatggg taataaactg atgtatcatg ttaccgttgg
    12721 aatatccgaa aaagaatatg atgcctttgc tattgcatcg tctcaaacca atctcctcca
    12781 ctcttcaaag tgggcacaag taaaatcaaa ttggcaaaat gaacgtttag gcttttataa
    12841 agatgaccaa ttagttgctg tagctagcat cttaattaaa tcattacctc ttggttttac
    12901 aatgctttat attccacgtg gacctattat agactacagc aataaagaat tagttggctt
    12961 tgtgttaaaa acactaaaaa actttggacg aaagaaacgt gctgtttttg caaaattcga
    13021 tccagcactt cttctacgtc aatatcacct taaagaagag aatgtcgccg aagagataga
    13081 tgaaagtaga caggcaattg ataatcttaa gtcagcaggt gcccaatgga taggtccaac
    13141 gaaagccatt tccgaaacca tccaaccacg ttttcaagcg aacatctata ccaaagcaaa
    13201 tatagaagaa aactttccaa aacacactaa acgtctgata aaagatgcaa agcatcgcgg
    13261 tgttcaaatt tatcgtgcca acattgatga tttacctaaa ttcgccacgg ttgtagcatt
    13321 aaccgagaat cgtaaaggtg tagctttacg taatgaaaac tatttccatc aactaatgac
    13381 aatttatgga gaagatgcct atctctattt ggctaaagtc aatcttccta aaagattagc
    13441 gcaatttaaa gaacaacttc ttcaaatcca aagagattta tcagaaactc catcccatca
    13501 aaaaagtcgc ctgacaaggc taaatcaaca agaagcttct gttaaacaat atatccttga
    13561 atttcaggag tttagcaaga aatatccaga tgagcctgtc attgcaggaa ttttgtccat
    13621 tcgttttggc aatgtcctgg agatgttgta tgctggtatg gatgatagct tccgtaaatt
    13681 ttatccacag tatctcttga atgctagggt gttcgaagat gctttcaaaa acggtattgt
    13741 atctgctaat ctaggcggtg tcgagggaag tcttaacgat ggtttaacaa aatttaaaag
    13801 taacttcaat ccaatgtttg aagaatatat tggcgagttt aatctagcaa tcaatccatt
    13861 attatataaa ttggccaacc tagcctatac tattcgaaaa aaacaaagac actctcacta
    13921 agaaaggaaa ctcatgtata gagaaattac cgctgtcgaa cacgatcgct ttgtgagcga
    13981 atccaaccaa acaaacctac ttcaatcttc taattggccc aaagtaaaag acaactgggg
    14041 tagtcaatta cttggctttt ttgacggtga aacccaaatt gccagcgcta gtattctcat
    14101 caaatcactt cctcttggct tctccatgct gtatattccg cgtggaccaa tcatggatta
    14161 ctccaatcta gatattgtaa ctaaggtcct taaggacctt aaagcttttg gcaaaaaaca
    14221 aagagctctc tttatcaagt gtgatcctct catctattta aaaatggtca atgccaaaga
    14281 ttttgaaaat tcgcccgatg aaaaagaagg gttaatcgcc attgatcatt tacagcgtgc
    14341 aggtgctgat tggactggtc ggacaacaga tttagctcat actatccaac cacgttttca
    14401 agcaaaccta tatgctaatc aatttggact tgataaaatg tctaaaaaaa ctcgtcaagc
    14461 tattcgaact tccaaaaata agggagtaga tatacaattt ggtagccatg aactacttga
    14521 agattttgca gagttaatga aaaaaacaga agaccgtaaa gggataaatc ttagaggaat
    14581 tgactactac caaaaactcc ttgatacgta tcctaataac tcatacatca caatggcttc
    14641 gctagatgtt gcaaaacgat tagaaaaaat agaaaaagaa tgccagatag ctcaatctga
    14701 aagaataaaa tcacttgaac ttaatcgtga gaaaaaagtt aaacaacacc aagatacgat
    14761 tgatcgatta aataaagaaa ttgattttct caaagaagct caaaaagcct atgaccgaga
    14821 cattgttcca ttggctgcaa ccctcactct agaatttggt aatacatcag aaaatattta
    14881 tgctggtatg gacgattact tcaaatctta ttctgctcct atttacactt ggtttgaaac
    14941 agctcaaagg gcttttgaac gtggaaatat ttggcaaaat atggggggga ttgagaatga
    15001 cttatctggt ggtttatatc attttaagtc taagttcgaa ccaatcattg aggaatttat
    15061 tggagaattt aacattccag tgaacagatt gctttataaa gcctctaatt atgtctatgc
    15121 cttacgcaaa aaacgtaata gctagttagg aaaggatact tttatggcgc ttaaagaatt
    15181 aacagctaaa gagttcgaaa gttattcagg aaattacgat ttacaatcgt ttatgcaaac
    15241 ccctgagatg gcaaagcttc taaaaaaacg tgggtatgac attacctaca tgggctacca
    15301 aatcgatggg aaaatggaaa tcatcagtat tgtttatacc atcccaatga ctggtggact
    15361 tcatatggaa gtcaattctg gtccagccca ttccaattct aaatatctga aacattttta
    15421 taaagaacta cagaactatg ccaaatctca gggcgcttta gaattattaa ttaaacctta
    15481 tgatacctac caagagttta ctggtgaagg tgaacctaaa ggtgcaccga acacctatct
    15541 tattgatggc ctgactagta taggttatca ccacgatggg cttcatattg gttatcctgg
    15601 cggtgaacct gattggcatt atgttaaaaa tctagaaggt atcactcctc aaaatcttct
    15661 taaatctttc tctaaaaaag gacgcccctt ggttaagaaa gccatgtctt ttggtataaa
    15721 aatacgtgtc ctcaagcggg aagaactaca tatctttaag gatattactt cttctacttc
    15781 tgatcgccgg gactatatgg ataaatctct agattattat caagattttt atgatagttt
    15841 cggagataag gcggaatttg tcattgcaac tctaaacttt agggaatatg atcataacct
    15901 acaattaaat gcaaagaaat tggaagaaca gattacagtc cttgataatc gccatcaaaa
    15961 taatacagat tctgccaaat accatcgtca acgtactgag cttgtcaatc aactagctag
    16021 tttagataaa cgtcgcaaag aagtcgaacc ttttattcaa aaatttggaa atcaagatgt
    16081 tgttttagct ggtagcctct ttatttatag ccctaaagaa actgtctatc ttttctctgg
    16141 ctcatacaca gaatttaata aattttatgc gcctgcagtt cttcaagaat atgttatgca
    16201 agaggctcta aagcgtcaat caactttcta taatttttta ggtattcaag gaaattttga
    16261 tggttcagat ggcgtacttc gctttaaaca gaattttaat ggttacattg ttcgtaaaat
    16321 gggtactttc cgctactatc caaatccatt aaaatataag agtattcaat tactaaagaa
    16381 aatcttacgc cgaacataaa aaataagctt acataagctt atttttttat gcattataga
    16441 aagcaacatt tatttcttta ctattattgt ttacacaaat aataacgttc gttatttagc
    16501 gaaattttat taccaatatc attattttct tgccgctctg ttaatcaagt gataaaatag
    16561 taaaaattta tcttcaggag gatattatgg tatcgctaaa aacggaaatt gctggtttta
    16621 gttttgacaa ttgtttgatg aatgcagcgg gtatttattg tatgactaag gaagagttat
    16681 tagctattga aaattctgaa gctggttcct ttgttacaaa aacaggtacc cttaaagcta
    16741 gagagggaaa ccctcaacca cgttatgcag atactgactg gggatcaatt aattctatgg
    16801 gactccctaa taaaggtatt gactattatc ttgatttcgt gactgaatta caagaccaag
    16861 acaatagcaa aaatcacgtt ttatcattag taggtttatc accagaagag acacatatta
    16921 ttcttaaaaa ggttgaaaac agtagttaca atggcctgat agagttaaat ttatcgtgcc
    16981 caaatgttcc tggaaaacct caaattgctt atgattttga aatgactgac ctcatccttt
    17041 cagaaatctt tagctattat caaaagccat tgggcatcaa attaccacct tactttgata
    17101 tctttcactt tgatcaagca gctacaattt ttaacaaata tcctttagct tttattaact
    17161 gtgttaattc tatcggaaat ggacttgtta ttgatgatga aactgtcgtt ataaagccta
    17221 aaaatggctt tggaggtatc ggcggtgatt ttattaagcc taccgcctta gctaacgtcc
    17281 atgctttcta caaacgttta aatccttcaa ttaaaattat cggaacaggt ggtgttaaaa
    17341 acggccgtga tgcctttgaa cacattctat gtggagctag tatggtccaa atcggaaccg
    17401 ctttacaaaa agaaggacca gaaatttttc agcgtgtaag cagagagtta aaagaaataa
    17461 tggctgataa aggctatcaa tcattggagg attttcgagg acaacttaat tacctttaaa
    17521 aataataaaa gagcaaacct tgtttgctct tttatattga ttttaccaat ctttatcaaa
    17581 ttcacgttct tcacgttcct cgggtgttaa atcatccttt acaggctttg ttatgatgtt
    17641 tccttcatta tctactttag gttttatcgt attcatatat aactttaaca gagctatctt
    17701 tataatagcg a
//
LOCUS       DATRNX010000037        17403 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRNX010000037 DATRNX010000000
VERSION     DATRNX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(97..2925)
                     /gene="uvrA"
                     /locus_tag="VBC81_000998"
     CDS             complement(97..2925)
                     /gene="uvrA"
                     /locus_tag="VBC81_000998"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6912047.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(3057..3728)
                     /locus_tag="VBC81_000999"
     CDS             complement(3057..3728)
                     /locus_tag="VBC81_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6912048.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(3753..4697)
                     /locus_tag="VBC81_001000"
     CDS             complement(3753..4697)
                     /locus_tag="VBC81_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6912049.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(4867..5106)
                     /locus_tag="VBC81_001001"
     CDS             complement(4867..5106)
                     /locus_tag="VBC81_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6912050.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(5151..5642)
                     /locus_tag="VBC81_001002"
     CDS             complement(5151..5642)
                     /locus_tag="VBC81_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6912051.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(5654..5941)
                     /locus_tag="VBC81_001003"
     CDS             complement(5654..5941)
                     /locus_tag="VBC81_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6912052.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            7035..8159
                     /gene="mutY"
                     /locus_tag="VBC81_001004"
     CDS             7035..8159
                     /gene="mutY"
                     /locus_tag="VBC81_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6912053.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            8336..8929
                     /locus_tag="VBC81_001005"
     CDS             8336..8929
                     /locus_tag="VBC81_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912054.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(8975..9289)
                     /gene="trxA"
                     /locus_tag="VBC81_001006"
     CDS             complement(8975..9289)
                     /gene="trxA"
                     /locus_tag="VBC81_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6912055.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(9370..9870)
                     /locus_tag="VBC81_001007"
     CDS             complement(9370..9870)
                     /locus_tag="VBC81_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6912056.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(9874..12213)
                     /locus_tag="VBC81_001008"
     CDS             complement(9874..12213)
                     /locus_tag="VBC81_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6912057.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(12298..12840)
                     /locus_tag="VBC81_001009"
     CDS             complement(12298..12840)
                     /locus_tag="VBC81_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6912058.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(12843..13154)
                     /locus_tag="VBC81_001010"
     CDS             complement(12843..13154)
                     /locus_tag="VBC81_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912059.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            13265..14158
                     /locus_tag="VBC81_001011"
     CDS             13265..14158
                     /locus_tag="VBC81_001011"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6912060.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            14174..14767
                     /gene="lepB"
                     /locus_tag="VBC81_001012"
     CDS             14174..14767
                     /gene="lepB"
                     /locus_tag="VBC81_001012"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6912061.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            14896..17316
                     /locus_tag="VBC81_001013"
     CDS             14896..17316
                     /locus_tag="VBC81_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6912062.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
BASE COUNT         5727 a         3332 c         2818 g         5526 t
ORIGIN      
        1 actatttttc ctaataaagc gttctccatt taatgacttc tacgcaatgt tagcttacaa
       61 aaaggaactg agtaatatta ctcagctccc aataaactat cttgctaact tctcttttaa
      121 ataatatcct gtataggatt taggattttc agcaacttct tctggtgttc cgatagctac
      181 aatttgtccg ccaccaatac ctccttcagg gccaagatca ataatgtggt cagcagtttt
      241 aataacatca aggttatgct caataacaag cactgtatta ccatcatcta caaaacgatc
      301 caaaacctta agtaaacgag caatatcatc tgcatgaagc cccgttgttg gttcgtctaa
      361 tatgtaaaga ctcttacctg tcgaacgctt atgtaattca cttgccagct tcattcgctg
      421 agcttctcct cctgataagg ttgtagctgg ttgccccaaa gtgacatatc ccaaaccaac
      481 atccttaata gtctgtaact tacgagcaat tttaggaata gctgcaaaaa atgtaactgc
      541 atcattcact gtcatatcaa gaatttgtgc aatatttttt tctttgtaat gtacttccag
      601 agtctcacta ttatagcgcg tgccgtggca tacttcacaa ggaacgtaga catctggtaa
      661 aaaatgcatc tcaattttga tgataccatc gccagaacaa gactcacaac gtccaccttt
      721 gacgttaaat gagaagcgtc cttttttata acctcgaatc tttgcctcat tagtttgagc
      781 aaaaagatca cgaatatcat caaaaacacc tgtataggtt gcgggattag aacgtggagt
      841 ccgaccaatt ggactttgat caatatcaat caatcgatca acatattcaa tgccttctaa
      901 agaaacatat ttacctggtt tatctgagtt acggttaagc ttctgcgcaa cagctttttt
      961 caaaatacta ttaattaagg ttgattttcc agaacctgag acaccagtta cggcaataaa
     1021 tttaccgaga gggaacttga catctaaatt ttgtaggttg ttttctgccg cacctttgat
     1081 ttctaaaaag cgaccattac ctactcttcg ttcagatggc acaggaatca ctttcttacc
     1141 tgacaaatat tgtcctgtaa tagatttagt atttttagct acttgcttcg gtgtaccaga
     1201 ggccacaatc tcaccaccaa acgcaccagc gccaggtccc acatcgatta accaatcagc
     1261 tgccatcatt gtatcttcat catgttcaac aacaataagc gtattaccca aatcacgcat
     1321 tttcttaaga ctatcaatca aacggtcatt gtctcgctga tgaagtccaa tggacggctc
     1381 atcaagtaca taaaggacac ctgataaatt agacccaatt tgagtagcca aacgaatacg
     1441 ttgactctcg cccccggaaa gtgttcctgc agaacgtgac agatttaaat aattaagtcc
     1501 aacattattt aagaaactta gacggtcatg gatttctttg ataattggtc gagcaattaa
     1561 ttgttcattt gaagataaac gcaatgtttc taaaagttct aaatggtctg ctatcgataa
     1621 atctgagact tgcccaatat taagaccttc ttctccccca actcttacgc aaagtgcttg
     1681 atcattgaga cgatagccat gacaagtgtt acattttagc tcgttcatat attctcgcat
     1741 aacattgcgt gtgtaatcgc tattagtttc atggtaacga cgattaatat tattgacaac
     1801 cccttcaaaa ggcaaatcaa tatttctctc gcctccaaaa tcgttaatat aatggaaatg
     1861 gaactctcgt tcgcctgaac catatagggc taggtcctgc tccgccttgc ttaatttttc
     1921 aaatggcgta tccacatcaa caccaaattg agtcatagct tgctctagca tcgttggata
     1981 ataatttgat gaaattggat tccaaggaac taaggcccct tctctaaggg tcttacttct
     2041 atctggaata accaaatcta tatctacttc taacttaatt cctaagccat cacaggtcgg
     2101 acaagaacca aagggagcat taaaagaaaa aaggcgtggc tctaattcag gaacagtaaa
     2161 accacattca ggacaagaat aatgctctga aaacaataac tcattgccat ccattgtatc
     2221 aataacgaca taaccatccg acaaacgtag agccgcttca acggaatcaa ataagcggga
     2281 acgaatgccc tctttattaa ttaatcgatc tactacaata tcaatattgt gcatcttact
     2341 ttttgataat tcaggcacct cagtaacatc gtgaatatca ccatcaatac gaacacgaac
     2401 gtaaccatct ttttgtattt tctcaaatgt tgatttgtgt tgtccttttt ttcgacgtat
     2461 aattggggct aaaatttgca tctttgttcg ctctggaagc gctagtacct tatccacaat
     2521 ttgctctaca gaagatgctg tgatggctcc gtgaccattg atacaatatg gggttccaac
     2581 acgagcataa agcaaacgta aataatcgtt aatttctgtt acagtcccaa cagtagagcg
     2641 aggattctta cttgttgttt tctgatcaat agaaatagct ggactaaggc catcaataga
     2701 atctacatca ggcttttcca tatttcccaa aaattggcgc gcgtaagcag aaagactttc
     2761 aacgtaacga cgttgtcctt ctgcatagat agtgtcaaag gccaaactag acttacctga
     2821 tcctgatagt cccgtaacga caactaactt gtctcgtgga atgtcgacac ttatattttt
     2881 taaattgtga gctcttgctc ctcttatcat taatttatct tgcataatgt tgtgcctcag
     2941 cactcctttt tgaatagtgt tgacttatta tatcaaattt taataaagtc agtatttttt
     3001 tagttttaaa tttcatagaa aaaagagatg aactaatacg ttcatactct ttgttattat
     3061 cttctttgtt gtggtgccac tggtgccatt gagctttgaa tattatattt acgttgtaaa
     3121 taaaagcgca aaccaaacga tgctaaagca ataataaaca aaacaacctc aggaagttta
     3181 ggattaatag ttgcaggtag aagaggtact actgcaaata aggcaaacca aactaacata
     3241 actagtgcca gtactgcgaa agatttaagc cagcctggtc tttcagattt tggtttaccc
     3301 atatggcgat aaatatagta ataagtaacg tacatggccg caccaccacc aaaaccaaga
     3361 gttaaaattg aaataagccc tgtattgaca ttagattgac caaatagcaa cattaaacca
     3421 ttcaaagcag ttactaagcc aagaaatagt aaggaagtat ctaaccacat caaccaagga
     3481 ttggtatttt tttgtacaga tactctagct ttatcctcag gtcgctcagt gaatgaggct
     3541 gcccaagtag ttggtgctcc taagagtgag cgtgcggtaa ttcctttttt ctgattatct
     3601 ataatctctg ggagatgttc ttctaaaata gctttgattt ctgcatctga ttttccatca
     3661 gctaaaagtt gattagttgc aatatgaaca aactcttgat tctttttagt taagtcttgt
     3721 aaagtcatgt gaactccttg attaatatac tattaaaacc attttttacg aataaaataa
     3781 aatacaacaa atgaactcat caaaaaggcc aaaagaacaa catagataaa gccatgtgcg
     3841 agaccattca aaggcatcca attattttta aagttcatcc catacgccga aaaaataacc
     3901 gttggaatat ccaatgtcat agttactagg gctaaggttt tcataatggt attctgatta
     3961 ttaccgatga ttgaagcagt cgtttctgtc atggcattta agacattttc atagatattt
     4021 gccatttcaa tagcctgctg agtttcgatc aatgtatctt caagcagatc ttcatcttca
     4081 atgtattttt ttaaactact ggtactactg gtcaattttt tgacgattcg ctcattaaat
     4141 tttaaagagg cctttagata gacaatagat ttttccaatt ccatcatatc gatcaattgc
     4201 tcatttcgag ttgcactctc tagttgtgct tcaattttat cgctctgcct atcgatagta
     4261 cgcaaagcct gtaagtataa ttcggcattg cgataaagca attggaaaac aaaacgtgtc
     4321 ttcataaagg tataaaaatt tttgacacgt cgtctataaa agtgatcaaa cagcgtcaaa
     4381 tgttctaggc aagtagtaat aacagcgttg tcagtaacga taatgcccat aggaatagtc
     4441 atataataac ttttgttatt gcgttcttca taggtaggaa cgtcaacaat aactaaagta
     4501 taatcatctt caaccgagat acgggaagtt tcttccacat cgaggggagc acgtaaatca
     4561 tcaatatcaa taccaaattg atcagctact tgttcggatt cttcctggga gggattaacc
     4621 agatttatcc aagcacctgg ctcgaatgtt tcaatttctt taaattcaat tgccgttgaa
     4681 agaaacattt gcttcatggc gattcctcct ttgttataaa aaatccatac cttagtatta
     4741 tacaggaaaa ccatttgttt tgcacggtta atacggtggt atcctaaatt tttctattat
     4801 ttgactcgta aataccaata ataatggtaa aaagaataaa aaaatagccg ctagggctat
     4861 tttatattaa tcttcattta cgtaaggcat aagagccata acacgagcac gtttgattgc
     4921 tgttgttact ttacgttggt ttttagctga agttcctgtt acacgacgtg gtaaaatttt
     4981 tccacgttct gaaacgaaac ggctaagaag ctctgtatct ttgtaatcaa catattcaat
     5041 cttgttagct gcgatataat caactttttt acggcgtttg aatccgccac gacgttgttg
     5101 agccatgttt atttctcctt tatagtttta aattctgtcc atctattata ttagaatggt
     5161 agatcatcat ctgaaatatc cattggattt gagttaccaa acggactctc atcacgacta
     5221 aaattaggtg tttgttgaga agaagctgaa taactattat ttgacggagc attgttatag
     5281 ccaccgttat tataagagtt aggtgaacca ccttctcgtg tagcacgact ttccaataat
     5341 tggaaacttt ccgcaacaac ttctgttaca tagacacgtt gaccttgttg gttttcataa
     5401 ttacgcgttt ggatacgacc tgtaattcca accaaagcac ctttttttgc ccagttagcc
     5461 aagttttcag cttgttggcg ccaaataaca cagttaataa aatcagcctc acgttcgcca
     5521 gattgatttt taaaattacg attaactgca agtgaaaaag tggctaccgc ttgattactt
     5581 ggtgtataac gaagttctgc atcacgggtc atgcgaccta caagtactac attattaatc
     5641 ataaactacc ttcttagtct actttaacga tcatgtgacg aagaatgtca ccgttgattt
     5701 ttgaaagacg atcaaactca ttaagagcta ctgcatcttc agcttcaacg ttaacgatat
     5761 ggtaaagtcc ttcagtgaaa tcttggattt cgtatgcaag acgacgtttt tcccaatctt
     5821 ttgattcaac aacagttgca ccgttgtcag ataagataga atcaaaacgt gctaccaaag
     5881 cgtttttagc ttcttcttca atgtttggac gaataatata aagaatttcg tatttagcca
     5941 ttgatatgtt cctccttttg gtctaatgac tcatgaattt ttcatgagta agtgaggtta
     6001 tgctcacaag atattattat agtagaaata agttaataat gcaaggtttt tatgttttta
     6061 tttccctgat atcatttaaa gacatttttt atttttcttt attgaaatat gccttatatc
     6121 aaacctatgt agccattttc ttttactgac ttatgttcaa tccccattct ataattcaat
     6181 taaaaaataa aaaggcgagg tttacacctc accttcaaca tattatttaa agaattcttt
     6241 attggtttaa gttttcgttg gagaaaaatt cagtgtctcc gattttatat ttttaaatat
     6301 taaaatacta tttagggagt ccctctttat gttttattac ctttggtaac accaaaacat
     6361 acacgaactt ttattcatat gtgttaccat tttacatctt tcatattgag atatttgatt
     6421 agcaatacct ccttgactaa caaaaattac taacacatct aggggtatac cctagtttat
     6481 atttgttcat gattacctca agcgagcatg cgctaatggt aatgaactat taccacattg
     6541 tctgtcttcc ttaaattata taaggactta tgtgtcaccg ttactgtagc gacctacatc
     6601 taaacctaac ataagtgccc ctccaactat ttctaggaaa tgaagaaaac ttcaaatcct
     6661 tgtgactgtt tgcaacatca atgcattgat aacaatttta gtgtatcgcc attcttatag
     6721 cgacccatat taaaaattaa cataagcgct acctccatta actaaagtta gcttgcctac
     6781 caatctttct tttggagact agtctccatt cttataccta gagatattgt aatagatcat
     6841 ggataacctc cttgatttaa actttatctt tataatttaa ttatagtgta aacgcttcct
     6901 taagtcaaga aaaaaatatt gatgcaacat ttttcttcat cattaagtac gatctagtca
     6961 acatacctct ttgtcaaatc ctctttcttt gatacaatta atctatgata gatttaaaag
     7021 actatggaat tgagatgtgg cctgaagata gaatcgcttc ttttcgtcgc acacttttag
     7081 ggtggtatga ccaagaaaaa cgtgatctcc cttggcgaag aactactaat ccatactata
     7141 tttgggtttc tgaaatcatg ttgcaacaaa cacaagttaa tacggtaatc ccttattata
     7201 aacgttttct agagtggttt cctcaaataa aagatttggc agacgctcct gaagaacaat
     7261 tactaaaggc atgggaggga ctaggctatt attctcgggt gcgtaatatg caaaaggctg
     7321 cacagcaagt gatggttgat tttggtggca tatttcctca cacttatgat gatattgctt
     7381 cattaaaagg gattggtcct tatactgcag gtgctatcgc cagcatctca ttcaacttac
     7441 cagagcctgc tgttgatggc aatgtgatgc gtgtcatggc tcgtttgttt gaagttaatt
     7501 atgacatcgg tgaccctaaa aatcgtaaaa tttttcaagc tattatggaa atactaattg
     7561 accctgacag acccggtgat tttaaccaag cacttatgga tctgggtaca gacatcgaat
     7621 ccgctaagac acctagacct gatgaaagcc ctattcgttt ctttaatgct gcttatttga
     7681 atggtactta tagtaaatat cccataaaaa atcctaagaa aaagcctaaa ccaatgcgaa
     7741 ttcaggcttt tgtcattcgt aaccaaaatg gtcaatatct actggagaaa aatactaaag
     7801 ggagacttct agggggtttt tggtcatttc ctattatcga aacgtcacct ctttctcaac
     7861 aattagattt atttgatgac aatcaatcca accccataat ctggcaaacc caaaatgaaa
     7921 ctttccaaag agaatatcaa ttaaagcccc aatggactga taatcacttt ccaaatatta
     7981 agcacacttt tagtcaccag aagtggacaa tagaactcat agagggggtg gtcaaagcaa
     8041 cagatttacc taatgctcct cacctaaagt gggtagcaat tgaggacttt tctctgtatc
     8101 cctttgcaac accacaaaag aaaatgctag agacctactt aaaacagaaa aatgcttaat
     8161 cacaagtgaa ctatgacggt tcactttttg tttacaattc ctttacgccg ctgtaaagtt
     8221 ttcctcagat tatcttttaa taccctataa atagtggtct atccctaatg tcaaatacat
     8281 ttgatagacc cgttagctta tcatccttat actaatatta tgaaaggaga tatttatgga
     8341 aattggtcag caaattattc gctaccgtaa acaacaagcg ctctcgcaag aagaattagc
     8401 cgagaaagtc tatgtaagcc gtcagagcat atctaactgg gaaaatgaca aaacttaccc
     8461 agacatccat agcctcttac tcctaagtca aatttttcaa gtgagcttag accaactaat
     8521 taaaggagac attgagaaaa tgaaatatac catcactcag gttgataaaa agaattttga
     8581 acgagatacc aaagtaatgg tgacattgat gatactatta atgatttcaa gttatccatt
     8641 agtctatttc ttagagtggc taggacttgg catttttgtt ttattatcga taattactat
     8701 gacttatgct aatcgtgtgg aacgttttaa gaaaaaatat gatgtgcaaa cttataagga
     8761 aatcctagct gtttcaagtg gaaaattatt agacgaaatc gaaaaacggg aagaaagagc
     8821 aaaacttcct tatcagaaac cccttattgt cactgtattt ttcctcatta ctgttgccac
     8881 cttttttgca agtcgtttta ttttcacatg gttgttccat ttaagttagt agataataaa
     8941 ataaagctca gcgtgacact gggctttatt ttatttaact caattcagca ataatagctt
     9001 tcagttggtc tttagtatga acacctgcaa cttgtttaac cacttctccg tcttttttaa
     9061 acatcaatgt tggaattgac ataataccaa actgacgggc agtttccgga ttctcatcaa
     9121 catccatttt caaaattttg agttcatctt catcgatttc ttgagataac tgttctaaga
     9181 ttggtgcttg catacgacat ggaccacacc aagttgccca aaaatcaatg agtactaaac
     9241 cttctttagt ttcttctaca aatgtagcat ctgttacttc taatgccatc ttttttctcc
     9301 atttcatctt aatgataagc ctattgtaca agaatttagt tgtttttgaa actatttgct
     9361 acgaactcgc taaataaata aacaaagccc taaaatcaag ccaataaggt aacctacaat
     9421 aacatcctta ggatagtgaa tacctgctat cacacggcaa attgccaata gaaccgataa
     9481 tattaaacaa actattccaa agtaaacata atatcgtaac aaacacatac taatcatcgt
     9541 agctgaaaaa acatgtctgc taggcattga ccttccctca gtatccttat aaattaaagg
     9601 tttaatattc catttttcat atggtcgtgg aaaatcaagg cgtttcctaa tgtaagacaa
     9661 agctataaag ccaacagcag ggataattaa tagccttaac cataattgaa atgtcatccc
     9721 ttgccaaaga gtaaatatca gaaaagatgg atagagtatg tacatcattc ctgtgactat
     9781 ttttagtaaa aaatttaaaa gaattattaa ctgtggggat tttctaaaag gttgagatag
     9841 tttggcataa aattgttcat atgattccat aatttaccct aacgttacaa ttgtggcgcc
     9901 agatccccct gcattttgtg gggcataagc aaaatgctta acgtgcttat tacgacgaag
     9961 atattttgtc actccctcac ggataacgcc tgtaccaata ccatgaatga tatcaacttg
    10021 tcccatattg ttaagcaatg cttgatcaat aaaattatct aactcttgca tagcttcttc
    10081 gtatctttta cctctaagat caagtcgagc tcttggtcca gaactatcag cctttttaac
    10141 cacattaatc tgtttacttt tgactttctg ttcttcttgg actctaacaa gggtaaattc
    10201 atcttgtgtt aatgtcattt tgataattcc cacttgtgct tcccaacgtc catcttttaa
    10261 ttgactagtt aaggtacctc gctgtccata gctagtcact ataatatcat caccaattct
    10321 aggagcacga gctgctttaa tttttttagc cttatttaag actttatttt ttgataaatc
    10381 aacttgaggt gctaattttt ttatttgtgc cttagcatct ataatttcgt gaggttttaa
    10441 ttggctctta tcattgagtt tttttaagat agtatcactc tcattcaaag ccatatctac
    10501 aatttcttga gcttcttgat agattttttc taactcttta tcgcgctcat gtgaaaattc
    10561 attatagagt ttcttaaccg cacgattgaa tttgaggttt tcttgttcaa cttctttaat
    10621 atgatccagt cttctacgtg tctcaagtgt ctgtgcctct aactgttcaa taatacggtt
    10681 aacatctgag tcagaatctg tcatctgctt agcttgttta acaataaatg gagctaaacc
    10741 aaggcgagaa gcaatttcaa atgcatttga tcgtccagga actccttgca taaagcgata
    10801 cgtagggcta agcgtttcgg catcaaattc catgctcgca ttctctacaa aatttgtctc
    10861 aatcccataa gcttttaatt ctggatagtg cgtggtcgcc atcgttttga tattacttaa
    10921 cctaaggtgt tctaaaatag ccatagccaa actagcacct tcttgaggat ccgttcctgc
    10981 tcccagttca tcaaagagga ctaaactatt gtggtcagcc tcgtttaaaa tactgactat
    11041 gtgcgtcata tgactagaaa aagttgatag actttgttca atagattgct catcgccaat
    11101 atctgcaaag atattgttaa atactgcaat tttactacct ttatccgcta atactggcaa
    11161 accagactgt cccattaatt gtgctaaacc gagtgttttt agcataatcg tcttaccacc
    11221 agtattggga ccagtgatga caattgcagt taaatcttga tcaaaatgta agtcattagc
    11281 cacagggtta cttaacagag gatgacgaac attgattaat gctaacgtgc tgtcattaga
    11341 aatctcaggt atcgtcgcct tattatcaga cataaaaaga tatttagccc ttacaaaatc
    11401 aagatgccca agaatccatg cattatttct aatagtggcg acatggggtc ttaacaagtc
    11461 tgaaaatgcg tgtaaaatac gactttcttc atgacgttcg tcagctctaa gctgcgttat
    11521 ctcttcgttt agtgtaacta cagcacgagg ctcaatataa acagtacttc ctgaagaaga
    11581 gatgtcatga accacaccag aaatacgatt ccgataagta ttttttactg gtaggacact
    11641 tcgtccacta cgactagcga ttagattctc tgataaaagc tctgcttttt ccttaagcat
    11701 atcctgtaaa atctgacgaa cccgtcgttc actgtttgtt aattgacgac ggatacgctc
    11761 taattctgga ctcgcaaaat gttctaaaaa accaccatca ttgatagctt gaaaagaccc
    11821 ttgcagatta gggaattgtt ccaaattttc aaacaaacga tccaaagatt ggaaagaaac
    11881 attatccaaa tcagaataaa agtgaatcat atccgaagaa ctctgtaaaa cttttttgat
    11941 agccaaaagt tcttgaatat taagatcagc tgaaagctct aagcgtttta aactctcaga
    12001 gattgaactt aggctgacta tgccaaatga gtgatgttca acaaaaatct gttccatgtc
    12061 agaaatttca ttaaaacttt tttctatttt aggagcctct gtcatcggct ccagctctga
    12121 taattcttct tgtgattgtt ctgtcttgag ataaggtaat atcaattcct taactttgtt
    12181 aaattctaac tgttctaaaa tcttgttatt catactcttc attataccta aatttatcac
    12241 aaaatgacaa cgttaaaaag gttggaactc ttactccaac cttactaaac attatattca
    12301 aagacctacc gcccaagtcc ttaccaaaaa ctgtgagaag aatggtaagt cgttaattaa
    12361 aaatctaaca aacagactat tccataactt ttcttgaaca aatgacatag ggactgttgc
    12421 cagaatagtc aaacacatac tcataaataa gagacaaacc agaacggcta aaaaaccact
    12481 aataatatta ttttgcaagc tatcaaaatg ttcaactggt gctaaatgta gtaaaacacc
    12541 taacaagcga cctactaagt aagataaact gtagataaaa acaaaggcaa cacctgcata
    12601 ataaacatga ctcaattgaa atttgctaat atctttaaaa aagtaaactt ctactccttg
    12661 tacaggatta gaatacggta cccataacgt aatctgacta gctaattctt ggtaaaattg
    12721 actagcaatc atcaaagata ccattgacat taacacgtaa aagacttgta aaaaaatacc
    12781 tctactgtag ccaatataaa aatgccaaat aacaataatc agcaaaagca atgatagcat
    12841 gattaatcct cactcacttt ttctttaatg tccgaaatcg ttttggagcg taacttattg
    12901 agctcatctt ccattttttc aatgtcaatt tctcgactta gttgcactga taaagcatta
    12961 attgccatga gaatagcaat cgtttcatta tcagcattag gtaatttttc tttaatagct
    13021 tcatactttt ctgtagctac acgctcaatc tcttccataa aaaggttgtc tttatctgtc
    13081 gtcaatgtga gaggcttatc tccaaagaca aatttataac gatttttctg actattattt
    13141 atttttgttt ccatactata cctctgctag cattataccg taaaataggg ctttcgtaaa
    13201 attctacccc ttatcattac ttgtctattc ccttattcct cttttttatg atagaatatt
    13261 gtctatgaac acaatcgtca tgcaagcaga taaaaaattg caagaaaaga taagaaccga
    13321 tttagctcaa catcatataa gtaataacaa tccttatgtg gtattttcag ctaaaatatc
    13381 aggagctact gttcttttat atacttctgg aaaacttgtc ttccagggtt ctaacgcttc
    13441 acatatcgcc caaaagtatg gttttattga gcaaaaagaa agttgttcct ctgagagtca
    13501 agatattccc ataattggta ctgatgaagt gggaaatggc tcttactttg gtggactggc
    13561 agtggtagct agttttgtaa ctcctaagga ccatgcttac ctcaaaaaat taggtgttgg
    13621 tgattcaaaa acacttactg atcaaaaaat taagcaaatc gcgcctcttt tggaaaaagc
    13681 aattccacac aaagcattat tgttatcgcc tcaaaaatat aatcaagtgg ttagtccaaa
    13741 taacaaacat aatgctgtct ccgtaaaagt tgctttacac aatcaagcta tcttccttct
    13801 tcttcaagat ggttttgaac cagaaaaaat cgttattgat gcttttacta gctccaaaaa
    13861 ctatcaaaat tatttgaaaa atgaaaagaa tcaatttaaa caaacgatta ctctggaaga
    13921 aaaagctgaa aataaatatc tagctgtagc ggtcagttct attattgcac gtaatctatt
    13981 cttagaaaac cttaacaaat taagcgacga cgtaggatat aaacttccaa gtggagctgg
    14041 tcaccaatct gataaggtcg ctagccaatt actcaaagct tatggtatct cttcgcttga
    14101 acactgcgca aaacttcatt ttgccaacac caaaaaagca caagcattat taaaataaat
    14161 agcagggaaa attatgaaag aatttattaa agaatggggt gtctttatcc tcatcctctc
    14221 acttttttta ctatcgcgta tctttttatg gcaattcgtt aaagttgacg gacactccat
    14281 ggatccaact ttagctgaca aggaacagct agtagttctc aaacaaacaa aaatcaatcg
    14341 attcgatatt gtagtggcta acgaagaaga aggcggccaa aagaaaaaaa ttgttaaacg
    14401 tgtcattggt atgccaggtg atgtcatcaa atataaaaat gacaccttaa ctattaacaa
    14461 taaaaaaaca gaagaacctt acctcaagga atatactaaa ttatttaaaa aggataaatt
    14521 acaggaaaaa tattcgtata acccactttt ccaagaccta gcacaaagct ctaccgcttt
    14581 caccactgac agcaatggca gcagcgaatt tactactgtc gtgcctaaag gccactacta
    14641 tcttgttggt gatgaccgaa ttgtctctaa agatagtcgt gccgtcggtt ccttcaaaaa
    14701 atcaacgatt gtgggagagg ttaaattccg cttctggcca attcgtcgtt ttggaactat
    14761 caactaaagg aaattaaaag ttcacctgac aaaagttgaa atagcatatt atattgaaaa
    14821 tagggagtga caaatccatt taaaataggg atttacaatc actcccttta actgtttatt
    14881 aaaaaggaag gtacaatgga agtatttttt acaggaacca ttgaacgcat tatatttgaa
    14941 aatgccagca atttttttaa aatattactt ctggaaatcg aagatacgga tagcgatttt
    15001 gatgatgtag aagtaattat tactggtact atggccgacg ttatagaggg agaagaatat
    15061 actttttggg gaacactaac ccaacatcca aaatacggcg agcaactaca aagtgtacgc
    15121 tatgaaagag ccaagcctac ttcaggtggc cttgtgaaat acttttctag cgaacaattt
    15181 aaaggaattg gtaaaaagac tgcccaacgc atcgtagaac tctatggaga taataccatt
    15241 gataaaatcc ttgaatcccc tgaacagtta agtacaatct caggactatc aaaaattaat
    15301 agagaagcat tcatcgctaa actgaaactc aattatggaa cagagcaagt tttagctaaa
    15361 ctcgctgaat atgggttaag caatcgtgct gctattcaaa tatttgatca ctataaagaa
    15421 gaatcacttg aagtgataaa cgaaaatcct tatcaacttg ttgaggatat ccaaggtatc
    15481 ggttttaaaa tagctgacca acttgctgaa caagtcggta ttgaaagtga ttctcctaaa
    15541 cgctttcgtg ctgctattat tcatacttta gttgagtctt ctatggagca aggagatacc
    15601 tatattgagg ctcgtactct cttggaaaaa accattactc tccttgaaga agctcgtcaa
    15661 atcgaactag atccttcaat tgttgctaag gaattgacaa atttaattgc agaagataaa
    15721 gttcaacata ttggaactaa aatttttagt aatactctat tttttgccga agaaggcatc
    15781 aaaaaaaact tacaacgaat actaaatcag cctcttgata aacaactaaa tcacaaagat
    15841 attgaccgtg aaatcagaga tattcaaaaa tctttaaata tccactacga taatatccaa
    15901 gaaaaagcta ttagagaagc tttactaagt aaggtattta tcttgactgg tggcccagga
    15961 acaggtaaaa caacagttat aaatggtatt attgaagcct attccgagtt acatcatatt
    16021 gatttaaaca aaaatgatat tcctattgta cttgctgctc ctacaggtcg tgctgctaga
    16081 cgcatgaatg aacttaccgg tttaccgagt gctactattc ataggcattt gggattaaac
    16141 ggagatagtg actatcaaag tctagatgac tatctagatt gttccttaat tatcattgat
    16201 gaattttcaa tggttgacac ttggcttgct aatcaattat ttgacgcttt ggactcgcac
    16261 acacaagtta ttattgtcgg agatagtgat caattaccgt ctgtaggtcc aggacaagtt
    16321 ctagcagatc ttttaaatat aaatgcactt ccacacgtta aacttgaaaa gatttttaga
    16381 caatcagaag aatcaactat tgtgacttta gctaatcaaa tgagacaggg ctttttgcca
    16441 gaggacttta cagctaaaaa agccgatcgc tcatactttg aagcatctgc taatatcatc
    16501 ccaaatatga tttctaaaat tgttcaatct gcacttaaga gcggtattga agctcatgaa
    16561 attcaaattt tagctcctat gtatcgcggc caagcaggta ttaataatct taaccttatc
    16621 atgcaaaatc tcctcaatcc attaaaagat aataaccaat ttacttttaa tgatataaat
    16681 ttccgaatag gagataaagt gttacattta gtcaacgata cagagcttaa tgtttttaat
    16741 ggggatattg gttacataac ggaccttata ccagccaaat atactgagag caagcaagat
    16801 gagatttata tgacttttga cggtcaagaa gttatctatc aacgcaaaga atggcttaaa
    16861 ataacactag cctatgctat gagtattcat aaatcacagg gttctgaatt tcaagttgtt
    16921 attctgccaa taacgagaca gagtggtcga atgttacagc gaaaccttat ttatacagct
    16981 attacgagat ctaaaagtaa actaatactt ttaggagaaa ttggtgcatt tgatttcgct
    17041 gttaaaaacg agggggcaaa gcgcaatact tatctgatag aaagatttga aaacaagcaa
    17101 gaaatagcta attctcaaaa aattgaagat agctcaatag atcaaaagat tgacaacaca
    17161 ataattaata cgtccattcc aaaaacagca acaccaattg agcagactaa tctttcaaaa
    17221 ataacttatc gcctaactga agaaaattat ttaacaatcg atccaatgat tggtattaat
    17281 caacaagata taagtgcaat ttttgatagt aaataaattt tttcataaaa tcttgaaaac
    17341 tcttccaaat atgttataat atcattgtat taagaacgta gttcaaattt aatactttta
    17401 tta
//
LOCUS       DATRNX010000038        17065 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRNX010000038 DATRNX010000000
VERSION     DATRNX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            180..4292
                     /gene="cas9"
                     /locus_tag="VBC81_001014"
     CDS             180..4292
                     /gene="cas9"
                     /locus_tag="VBC81_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6912063.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4294..5163
                     /gene="cas1"
                     /locus_tag="VBC81_001015"
     CDS             4294..5163
                     /gene="cas1"
                     /locus_tag="VBC81_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6912064.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            5160..5501
                     /gene="cas2"
                     /locus_tag="VBC81_001016"
     CDS             5160..5501
                     /gene="cas2"
                     /locus_tag="VBC81_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6912065.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            5488..6153
                     /gene="csn2"
                     /locus_tag="VBC81_001017"
     CDS             5488..6153
                     /gene="csn2"
                     /locus_tag="VBC81_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6912066.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6254..6751
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6254..6289
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            7308..7724
                     /gene="ndk"
                     /locus_tag="VBC81_001018"
     CDS             7308..7724
                     /gene="ndk"
                     /locus_tag="VBC81_001018"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6912067.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            7860..9692
                     /gene="lepA"
                     /locus_tag="VBC81_001019"
     CDS             7860..9692
                     /gene="lepA"
                     /locus_tag="VBC81_001019"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6912068.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            9938..12571
                     /locus_tag="VBC81_001020"
     CDS             9938..12571
                     /locus_tag="VBC81_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6912069.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SLLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            12671..13330
                     /locus_tag="VBC81_001021"
     CDS             12671..13330
                     /locus_tag="VBC81_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6912070.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            13339..13803
                     /locus_tag="VBC81_001022"
     CDS             13339..13803
                     /locus_tag="VBC81_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912071.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            13803..14240
                     /gene="msrB"
                     /locus_tag="VBC81_001023"
     CDS             13803..14240
                     /gene="msrB"
                     /locus_tag="VBC81_001023"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6912072.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            14389..>17065
                     /locus_tag="VBC81_001024"
     CDS             14389..>17065
                     /locus_tag="VBC81_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6912073.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDNNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNL"
BASE COUNT         5801 a         2632 c         3343 g         5289 t
ORIGIN      
        1 tttcaagcta aatacggact ggattaaaaa atcactgcct tattttaacg tgctgtgctg
       61 tttcgaataa ttccaacaaa attattatat gttattttaa tgtaataatc aagtaaaaat
      121 tagcaaaact ttaaagtttg cgtttttaaa gtgatatttt aaataaaagg agataaatta
      181 tgaacaaacc atattcaata ggtctcgaca tcggaactaa ttccgtcgga tattctgtgg
      241 ttacagatga ttataaggta cctgctaaga agatgagggt cttagggaat acagataaag
      301 aatacataaa gaagaatctt ataggtgctc tgctttttga tggtggaaat actgcttcag
      361 atagacgctt gaagcgaact gctcgccgtc gttatacacg tcgtagaaat cgtattctat
      421 atttacaaga aatttttgca gaggaaatga gtaaagttga tgatagtttc tttcatcgat
      481 tagaggattc ttttctagtt gaggacgaca agagagggag caagtatcct atctttgcaa
      541 caatgcagga ggagaaagat tatcatgaaa agttcccaac gatttatcac ttgagaaaag
      601 aattagctga caagaaagaa aaagcagacc ttcgtcttgt ttatctggct ctagctcata
      661 tcattaaatt cagaggacat ttcctaattg aggatgatag ctttgatgtg aggaatactg
      721 atattcaaag acaatatcaa gcttttttag aaatctttga tactaccttc gaaaataacc
      781 atttgttatc tcaaaacata gatgtagagg gaattctaac agataagatt agcaagtctg
      841 cgaagaaaga tcgcatttta gcgcagtatc ctaaccaaaa atctactggc atttttgcag
      901 aatttttgaa attgattgtc ggaaatcaag ctgacttcaa gaaacatttc aatttggaag
      961 ataaaacgcc gcttcaattt gctaaggata gctacgatga agatttagaa aatcttcttg
     1021 ggcagattgg tgatgaattt gctgacttat tctcagtagc gaaaaagcta tatgatagtg
     1081 ttcttttatc tggcattctt acagtaactg atctcagtac caaggcgcca ctttctgcct
     1141 ctatgattca gcgttacgat gaacatagag aggacttgaa acagttaaaa caattcgtaa
     1201 aagcttcatt gcctgagaaa tatcaagaaa tatttactga ttcatcaaaa gatggctacg
     1261 ctggttatat tgaaggcaaa actaatcaag gagcatttta taaatacttg tcaaaattgt
     1321 tgaccaagca agaaggtagc gagtattttc ttgaaaaaat caagaatgaa gattttttga
     1381 gaaaacaaag gacctttgat aatggctcaa ttccacacca agtccatttg acagaattga
     1441 aggctattat tcgacgtcaa tcagaatact atccattctt gaaagagaat ctagatagga
     1501 ttgaaaaaat ccttaccttt agaattcctt attatgtcgg gccactagca cgtgagaaga
     1561 gtgattttgc atggatgact cgcaaaacag atgacagtat tcgaccttgg aattttgaag
     1621 aattggttga taaggaagca tctgcggaag cttttatcca tcgtatgacc aacaatgacc
     1681 tctatcttcc tgaagaaaaa gtcctaccaa aacatagtct tatttatgaa aaatttacgg
     1741 tctataatga gttgactaag gttagatata aaaatgagca aggtgagact tatttttttg
     1801 atagcaatat taaacaagaa atctttgatg gagtattcaa ggaacatcgt aaggtatcca
     1861 agaagaagtt gctagatttt ctggctaaag aatatgagga gtttaggata gtagatgtta
     1921 ttggtctaga taaagaaaat aaagctttca acgcctcatt gggaacttac cacgatctca
     1981 aaaaaatact agacaaagat tttcttgata atccagataa tgagtctatt ttggaagata
     2041 tcgtccaaac tctaacctta tttgaagata gagaaatgat taagaagcgt cttgaaaact
     2101 ataaagatct ttttacagag tcacaactaa aaaaactcta tcgccgtcac tatactggct
     2161 ggggacgatt gtctgctaag ttaattaatg gcattagaga taaagagagt caaaaaacaa
     2221 tcttggacta tctgatttct gatggaagag caaatcgcaa ctttatgcag ttgatacatg
     2281 atgatggtct atcatttaaa ccaattattg acaaggcaca ggctggtagt cattcagata
     2341 atctaaaaga agttgtaggt gagcttgcag gtagccctgc tattaaaaag gggattctac
     2401 aaagtttgaa aattgttgat gagcttgtta aagtcatggg atacgaacct gaacaagttg
     2461 tggttgagat ggcgcgtgag aatcaaacaa caaatcaagg tcgtcgtaac actcgacaac
     2521 gctataaact tcttgaggaa ggcgttaaga atctagctag tgacttgaat ggcaatattt
     2581 tgaaagaata tcctacggat aatcaagcgt tgcaaaatga aagacttttc ctttattact
     2641 tacaaaatgg aagagatatg tacacagggg aagcactaga tattgacaat ttaagtcaat
     2701 atgatattga tcacattgtt cctcaagctt tcataaaaga tgattctatt gataatcgtg
     2761 ttttggtatc atctgctaaa aaccgtggaa aatcagataa tgttcctagc attgatattg
     2821 taaaagcacg caaagcattc tggaaaaaat tacttgatgc taagttaatg agtcagcgta
     2881 agtatgataa tttgactaag gcagagcgcg gaggcctaac ttccgatgat aaggcaagat
     2941 ttatccaacg tcagttggtt gagacacgac aaattaccaa gcatgttgcc cgtatcttgg
     3001 atgaacgctt taataatgag cttgatagta aaggtagaag gattcgcaaa gttaaaattg
     3061 taaccttgaa gtcaaatttg gtttcaaatt tccgaaaaga atttggattc tataaaattc
     3121 gtgaagttaa tgattatcac catgcacatg atgcctatct taatgcagta gttgctaaag
     3181 ctattctaac caaatatcct cagttagagc cagaatttgt ctacggtgac tatccaaaat
     3241 ataacagtta caaaacgcgt aaatccgcta cagaaaagct atttttctat tcaaatatta
     3301 tgaacttctt taaaactaag gtaactttgg cggatggaac cgttgttgta aaagatgata
     3361 ttgaagttaa taatgatacg ggtgaaattg tttgggataa gaagaaacac tttgcgacag
     3421 ttagaaaagt cttgtcatac cctcaggtca atatcgtgaa gaagacagag attcagacag
     3481 gtggtttctc taaggaatca atcttggcgc atggtaactc agataagttg attccaagaa
     3541 aaacgaagga tatttattta gatcctaaga aatatggagg ttttgatagt ccgatagtag
     3601 cttactctgt tttagttgta gctgatatca aaaaaggtaa agcacaaaaa ctaaaaacag
     3661 ttacggaact tttaggaatt accatcatgg agagattcag atttgagaaa aatccatcag
     3721 ctttccttga atcaaaaggt tatttaaata ttagggacga taaattaatg attttaccga
     3781 agtatagtct gttcgaatta gaaaatgggc gtcgtcgatt acttgctagt gctggtgaat
     3841 tacaaaaagg taacgagcta gccttaccaa cacaatatat gaagttctta taccttgcaa
     3901 gtcgttacaa tgagctaaaa ggaaaaccag aagaaattga acaaaaacag gaatttgtag
     3961 ttcaacatgt ttcttatttc gacgatattc ttcaaataat caacgatttt tcaaatcgag
     4021 ttattctagc agatgctaat ttagagaaaa tcaataagct ttaccaagat aataaggaaa
     4081 atatatcagt agatgaactt gctaataata ttatcaatct atttactttt accagtctag
     4141 gagctccagc agcttttaaa ttttttgata aaatagttga tagaaaacgc tatacatcaa
     4201 ctaaagaagt acttaattct accctaattc atcaatctat tactggactt tatgaaacac
     4261 gtattgattt gggtaagtta ggagaagatt gatatggcag gttggcgaac cgttgttgta
     4321 aatacacatt ctaagctctc ttataaaaat aatcatctgg tttttaaaga ttcttatcag
     4381 acggaaatga ttcatctatc agagattgac attctaatca tggaaacaac agatatcgtt
     4441 ttgtcgacca tgctgattaa acgtttggtt gatgaaaata ttttagttat attttgtgat
     4501 gacaaacgct tgccaacagc tatgttaatg ccgtactatg ccagatatga ttcgagttta
     4561 caattatcta ggcagatgtc atggattgaa gatgtcaaag cggatgtttg gacgtcaatc
     4621 attgcacaaa aaattttgaa tcagtctttt tatcttggtg agtgttcttt ctttgaaaaa
     4681 tcccagtcta ttatgaatct ctatcatgat ttagaatctt ttgatacttc caatcgtgaa
     4741 gggcatgcag ctaggattta tttcaataca ctttttggaa atgatttttc aagagaacat
     4801 gataatccaa taaatgctgg tttagactac ggatattcat tgcttttgag tatgtttgcg
     4861 cgtgaagtcg ttaagtgtgg ttgcatgaca caatttgggt taaagcatgc taatcaattt
     4921 aatcagttca acctagcaag cgatattatg gaaccatttc gcccaatcgt tgataggatt
     4981 atttatgaaa ataggcagag tgattttgtc aaaatgaaaa gagaactctt ttctatgttt
     5041 tcagagacat acagttacga cggtaaagaa atgtatcttt caaatattgt cagcgattac
     5101 accaaaaaag ttattaagtc gctaaatagt gatgggaatg ggattccgga gtttaggata
     5161 tgagttatcg gtatatgaga atgattttaa tgtttgatat gcctactgaa acagtagaag
     5221 aacgtaaggc gtatcgtaag tttagaaagt ttcttttgag cgaaggtttt attatgcatc
     5281 agttctctgt ttatagtaaa ttattactca ataatacagc taataatgct atgataggtc
     5341 ggcttaaagt gaataatcct aagaagggta atatcacact cttaacagtt acggaaaaac
     5401 aatttgcgag aatggtttac ctccatggag aacgcaacac aagtgttgcc aactttgata
     5461 gtcgcttggt tttcctagga gattcttatg atcaagatta attttccaat tttagatgaa
     5521 ccattagtgt taagtaatgc tacgatttta acgatagaag atgtttcagt ttattcttca
     5581 ttggtgaaac atttttatca atatgacgta gatgaacatt tgaaattatt tgatgataag
     5641 cagaaaagtc tgaaggcaac agagttaatg atggttacag atatcttagg atacgatgtc
     5701 aactcagcac ctattctaaa gttgatacat ggtgacttag aaaatcaatt caacgaaaag
     5761 ccagaagtga aatcaatggt agaaaaatta gcagctacta ttacagaact tatcgcattt
     5821 gagtgtctag agaatgagct tgatttagaa tacgatgaaa tcacgatttt agaactcatt
     5881 aaggcactag gagtcaaaat tgagacacag agcgacacta tctttgaaaa atgttttgaa
     5941 attatacaag tttaccatta tttaacgaaa aagaatctct tggtttttgt taatagcgga
     6001 gcttatctta ccaaagatga agttataaaa ttatgtgaat acatcaattt aatgcaaaag
     6061 tcagtactct ttctagaacc tagaagactc tatgatttac cgcaatatgt tattgataag
     6121 gattatttct tgataggcga aaatatggta taatattagt aaaagcacag taataacaag
     6181 gaatcatcga aactgaagtc ctgctgagac gaatggcgcg attacgaaag ctcaaaagaa
     6241 aattttctac gaggttttag agctgtgctg tttcgaatgg ttccaaaact gtatgattaa
     6301 atccatcctt gtcatactgg ttttagagct gtgctgtttc gaatggttcc aaaacccaag
     6361 ccgttactgc aaacatcatg tcgctgtttt agagctgtgc tgtttcgaat ggttccaaaa
     6421 ctttagttcc tccttttaca aatacaatgt ggttttagag ctgtgctgtt tcgaatggtt
     6481 ccaaaactat cgatattgat gatttaacat caattatgtt ttagagctgt gctgtttcga
     6541 atggttccaa aactggtggt atttataatg tacgagcaaa tcggttttag agctgtgctg
     6601 tttcgaatgg ttccaaaacg ataaaaagtg ggagctgaat taaaaggcag ttttagagct
     6661 gtgctgtttc gaatggttcc aaaacatttg aacgattttt atattcctga tatgtgtttt
     6721 agagctgtgc ggttattatg ctagggcacc gttgtggtgt tctagttttt tgttatactg
     6781 aaataaattt tcagagaatg tgggggaagg cggtaattag attaattcaa gacgtaattc
     6841 agaacttagt tggccaagct aacgaaatca ccccagttta tcagtttgat tgggaaactt
     6901 atatattggc gactaaaaaa tatgaacgtc atttagaggt gtgtctatta gtagaaaatt
     6961 cgaattattt ttcggattca aagagaatgt gtcgatagaa gagatacgaa agactataat
     7021 tccaacctta tggttaaagg gctagattgt tctacgcttt acttaattat tagtttgaca
     7081 gcgttggttc ttttagtgat tgctggtgtt tctttgtcac taatacttgc aagcttacaa
     7141 ggaaagcagt ggagaaccta tccataatca actaacagct atgatggatc tcacctatta
     7201 atatagttaa ggagtgtccg acactccttt tttgaaatac agttggaagt tacagaaaac
     7261 atattttcat gttataatag tggagaaaga aatgaggtgc taatattatg gaacaaacct
     7321 tttttatgat aaaacctgat ggtgtaaaac gtggttttat tggagaagtt attagtcgta
     7381 ttgagcgtcg aggcttctct attgatcgct tagaggttag acatgcagat gcagatattt
     7441 tgaaaagaca ttatgcagaa ctgacggata gaccattctt tccaacatta gtagattata
     7501 tgactagtgg tccagtaatc attggagtta tttcgggaga agaggtcatt tcaacttggc
     7561 gcaccatgat ggggagtact aaccccaaag atgcccttcc aggaactatt cgaggagatt
     7621 ttgcacaagc tccctctcca aaccaagcga catgtaatat tgttcatggg tcagactcac
     7681 ctgaatctgc agctcgcgag atagccattt ggttcaataa ctaatagact taatgaagat
     7741 tgatgttcaa gtgttaattc tcttttacat cggtcttctt tttgatataa tattctagag
     7801 tctataggac tgttttaata ttttagaagt taaacatatc aaattgtata ggaaatagaa
     7861 tgaatattga agatttgaag aaacgtcaag aaaagattag aaacttttcg attatcgctc
     7921 atattgacca tggaaaatca accttagcag accgtatttt agagaaaaca gaaacggttt
     7981 ctagtcgtga aatgcaggcg caactgttgg actctatgga cctagaacgt gagcgtggga
     8041 ttactataaa gttaaatgct attgagctta attatactgc taaagatggt gaaacttaca
     8101 tttttcactt gattgacacg ccagggcatg ttgactttac atatgaagta tcacgttcat
     8161 tggcagcttg tgagggtgct atactggttg ttgatgcagc ccaaggaatt gaagctcaaa
     8221 ctctggcaaa tgtttatttg gctctagaca atgatttaga gatactacca gttattaata
     8281 aaattgattt accagcagca gatccagaac gtgtacgtgc agaagtagag gatgttattg
     8341 gtctagatgc ttcagaagct gtacttgcct cagctaaggc agggattggt attgaagaaa
     8401 ttttagagca gatcgttgaa aaagttcctg ctccgactgg ggaagtagat gctcctcttc
     8461 aagctctaat ttttgattct gtttacgatg cttatcgtgg agtaattctg caagtaagga
     8521 ttgtaaatgg tatggttaag ccaggagata agattcaaat gatgtctaat ggtaaaacct
     8581 tcgacgttac tgaggttggt attttcactc ccaaagcagt tggacgtgat tttttagcaa
     8641 ctggagatgt agggtatatt gctgcctcaa ttaaaacagt tgctgataca cgtgttggtg
     8701 ataccattac ccttgctaat aacccagcta tagacccctt gcatggctat aagcaaatga
     8761 acccaatggt ttttgccggt ctttatccaa ttgaatcaaa taagtataac gatctgcgtg
     8821 aagcccttga aaaattacaa ttgaatgatg ctagtctaca gtttgaaccg gaaacatcac
     8881 aagcattagg gtttggtttc cgttgtggtt tcttagggct tttacatatg gacgttatcc
     8941 aagagcgctt ggaacgagag ttcaatattg atcttattat gactgcacca tcagtagttt
     9001 atcatgttaa tacaactgat ggtgaaatgc ttgaagtttc gaatccatct gaatttcctg
     9061 atcctactcg tgttgacagc attgaagaac catatgttaa ggcgcaaatt atggttcctc
     9121 aagaatttgt cggtgctgta atggagttag cacaacgtaa acgtggtgac tttgtaacaa
     9181 tggattatat tgatgataat cgtgttaatg ttatttacca aattccttta gctgaaattg
     9241 tgtttgactt ctttgataag cttaaatcat caacacgtgg ctatgctagt tttgattatg
     9301 aaatttccga atatcgtcgc tcacagttag ttaaaatgga tattctttta aatggtgata
     9361 aagtggatgc tttaagcttt attgttcaca aagaatttgc ctatgaacgt ggtaaattga
     9421 tcgttgataa gttgaaaaaa atcattcctc gtcagcagtt tgaagttcct atccaagcag
     9481 ctattggaca aaaaattgtg gctcgttctg atataaaagc actacgcaaa aatgtcttag
     9541 caaaatgtta tggtggtgac gtttcacgta aacgtaagtt gcttgaaaaa caaaaagctg
     9601 gtaaaaagcg tatgaaagca attggttccg tcgaagttcc tcaggaagct ttcttaagtg
     9661 ttttatcaat ggacgatgat gacaaaaaat aattcagaaa gatgatatca tttatctatt
     9721 ttaaagtaac ttagcttcgt gcttaagtta ctttttcttt gtctgcaatt atcaaataat
     9781 atggctttat aatatcttta tttaaatata aatgagctat aaatgagttt ttgttagcaa
     9841 ataaataata aaaagatggc aaatagtatt gcaaaaagaa aaacatctga tataattaac
     9901 tagttaacta aaacgaaaaa acatttatgg aggactcatg aaaggtcaaa aaattattgc
     9961 tctagctggt cttgtcctgt catgccattt tgctcttacg gcatgtcata ctcaagaaca
    10021 caaaaattct tatcatatta aaacaaagca ggttgctaag aaaaaagcta ataaaaagaa
    10081 agtctctgtt aaggaaagtc ataaaaaacg caagggtgtt gcaggggttg actttccaac
    10141 tgatgatggc tttttactga ctaaagattc gaagatttta tctcatacag attctggaat
    10201 cgttgtagca catggaaatc attctcattt tattttttac tctgatttaa aaggttcaaa
    10261 atttagctat ttaatcccta atgctaatgc aaaaactaat aaaaatcaag cggtaagaaa
    10321 ctttaaagca ggggcggtag ctgttaatac attaaatgat ggctatgttt ttaatcccgc
    10381 agatattgtt tcagaagatg ctaatggcta cgtggtcaga catggtgacc atttccacta
    10441 tattccaaaa gcaagtttat ctcagcaaaa gcaagtacaa gcaagtagag ctgtttcacg
    10501 tttaggaaat caaagtaata gccattacag ggttaatagt tctaaaattg cagggcttca
    10561 ctacccaaca agtgatggtt tcttatttaa tggacgagga ataaaaggca ctaccccaac
    10621 aggtatttta gtagaacatc ataatcatct gcattttatt agttttgctg atttaagaaa
    10681 aggggggtgg ggatcaattg ctgaccgtta ccaaccacaa aagaaagctg attctaaaaa
    10741 acagtcacca tcaagtaaaa agccaagaaa tgaaaatact ttacctaagg acatcaaaga
    10801 taagctagct taccttgctc gtgaattaca tttagatatt tcacgtatca gagtccttaa
    10861 aacactaaat ggtgaaattg gatttgaata cccacatgat gatcatacac atgttattat
    10921 ggccaaagat attgatctaa gtaagccaat tccaaatcca catcatgatg acgaagatca
    10981 tcacaaaggt catcaccatg atgagtccga ccataagcat gaagaacacg agcacactaa
    11041 atcaaataaa ttatctgatg aaaatcaaaa gaaattaatt tatttagcag agaagcttgg
    11101 tttaaatcct aatcaaattg aagtattaac gtcagaggat ggaagtatca tctttaagta
    11161 tccacatgat gaccactcac acactatagc aagtaaggat atcgaaattg ggaagcctat
    11221 cccagatgga caccacgatc actctcatgc aaaagataaa gttggtatgg caactctaaa
    11281 acaaattggt tttgatgatg agattattca ggatattctg catgcagatg ctccaacacc
    11341 attcccatca aatgaaacta atcctgaaaa aatgcgtcag tggcttgcaa ctgttactaa
    11401 gattaacatt ggacaaagaa ccaatccatt ccagcgtttt ggtctatcat tgatgcctaa
    11461 tattgaagtt ctaggaattg ggtttactcc gattaatgat atgacgccag ttttgcaatt
    11521 caaaaaactt aaacagcttt ggatgacaaa tactggtatt acagattatt ctttccttga
    11581 taagatgcca ctattagaag gattggatat ttcacaaaac ggtattaagg atttatcttt
    11641 acttacaaaa tataaacaat taagtcttat tgctgcagct aataatggca ttacttcgct
    11701 aaaaccattg gctgaattac caaaccttca attcttagtt ttgagtcata acaatatctc
    11761 tgacttgaca ccgttatcaa acttgacaaa actccaagaa ttgcaccttg atcataataa
    11821 tgtgaagaat ttaagtgcac tttctggtaa aaaagatttg aaagttctag atttatcaaa
    11881 caataaatcc gcagacttat cgactctaaa aacaactagt cttgagacgc ttcttttaaa
    11941 tgagacgaat acaagcaatc taagtttctt aaagcaaaat cctaaggtat ctaatttaac
    12001 aattaataat gcgaaattat catcattaga tggtattgaa gagagcgatg aaattgttaa
    12061 agtagaagct gaagggaacc aaattaagtc actagtattg aaaaataaac aaggttcgtt
    12121 aaaattcttg aatgtgacta ataatcagtt aacatcactt gaaggtgtta ataattatac
    12181 ttcacttgag accttaagtg tttctagaaa taaacttaaa tctttagata ttaaaacacc
    12241 taacaaaaca gttacaaatc ttgattttag ccataacaat gttccaacat cacaattgaa
    12301 attgaatgaa aaaaatattc ctgaagcagt agctaagaat tttccagcag ttgtagaagg
    12361 ttcaatggtt ggaaatggta gtcttgctga aaaagcagct atggcttcta aagaggacaa
    12421 acaagtttca gataatacta atcaccaaaa aaacactgaa aaatctgctc aagcgaatgc
    12481 tgatagtaaa aaagaaaacc ctaaaacaca tgatgaacat catgaccatg aagaaacaga
    12541 tcatgcacat gtaggtcatc atcaccatta atcttttacg gagctaatat ataaaatcct
    12601 tcaagaattt cttggaggat tttgcattta tatgctaaat catttatttt tttagaaaat
    12661 tagctataat atgactatgg ttaaaagaga agatgtgatt ttaaatgcaa ctgaaaatat
    12721 ggtttaccat aaattaaaaa atgatccaag tggccatgac tggtttcata ttgttagagt
    12781 aagaaatctt gcagtagaat tagcacacaa agaaggtgct aataccttta tttgtcaaat
    12841 ggctgcactt ttgcatgata ttattgatga caaaatctgc caagatagta agcaagcgtc
    12901 gtatgaattg actcaatggt tatatagtca agatcttgcc atagcagaag tagaacatat
    12961 ccttgatatt cttgaaaata tttcattcaa agcaggaact ggattgacta tgaaaacatt
    13021 ggaaggtcaa attgttcaag atgcagatcg cctagatgct atgggggcta ttggtattgc
    13081 aagaacgatg gcttattcag ggagtaaagg acgtttaatt catgacccta acttaaaacc
    13141 acgagaaaac ctcactttag aagagtatcg taatggtcag gatacagcta tcatgcattt
    13201 ttacgaaaaa ttattaaagt taaaagacct tatgaataca aaacaaggga aaatgcttgc
    13261 tcaaaaacgt catgattttt tagaactata tttagccgaa ttttatgctg aatggaatgg
    13321 taaaggttag gagtagatat gattcgtaga gcgaaagaaa aagatttgcc tgctattgca
    13381 gagctattga aacaaatttt agtgttacat catggggttc gcccagatat ttttcataca
    13441 agaggaagta agttttctaa agaacaattg aaagaaatgc tgatggacga atcaaggcct
    13501 atctttgttt atgaatcaga cgagggtaaa gttgttgccc acttgtttct tcaattacaa
    13561 gaaaaacgtg acttacctcg aaagtccttt aaaactctct atatagatga tttgtgcatt
    13621 gatgaggaag taagaggtca gcaaattgga caaaagttga tggattttgc aagacaatat
    13681 gctaagaaac atgggtgtta taatataact ttaaatgttt ggaatgataa tcagagagcg
    13741 gtctcttttt atgaaaaatt aggttttaaa cctcaacaaa ctcaaatgga ggagatacta
    13801 taatgaaaga aacacaagaa gaattacgtc agcgtatcgg acatacagct tatcaagtaa
    13861 cccaaaattc agctacggaa catgctttta ctgggaagta tgatgatttt tttgaagagg
    13921 ggatatatgt agatatcgtc agtggagagg ttttattctc ttctttagat aaatttcaat
    13981 ctggttgtgg ttggccagca ttttcaaaac caattgagaa tcgaatggtg acaaatcatc
    14041 aagatcactc tcatggaatg catcgtattg aggttaggag tcgtcaagct gactctcatt
    14101 tagggcatgt atttaatgat ggaccggttg atgcaggtgg cttacgctat tgtatcaatt
    14161 cagctgcctt agattttatt ccttatgacc agatggcaaa gagaggctat ggggactact
    14221 tatcactttt tgatgaataa gagacgactt aagggtatat cttaagtcgt tttttatgta
    14281 tggtaaaaag aattggtggc aggtgtattc ttcatcagtt tattttctga aaaaagagta
    14341 taatataggt atcaaaaatg aaaacgtttt agagaaggag actagaatat ggcttctaca
    14401 aataatgttt cagatacaaa cacacgtttt gctaaagagg aattagaaga ggttttcgaa
    14461 gaattaggga caactcaagg tggtttatca gatgaagagg ttgctgtacg tcagaaaaaa
    14521 tatggtttaa acctgttatc tgaagtaaag caagaatcaa ttattttatt gtttctgaaa
    14581 aattttacta gtcttatggc aatactatta tgggttggag gatttgtggc tattgtttct
    14641 aatagcttag agctaggtct tgccatttgg atggttaatg tgattaatgg tatttttagt
    14701 ttcattcagg aataccgtgc aagccaagca acacaagctc tcaagaaaat gttaccatct
    14761 tattcacgtg ttcttagaaa aagctcagaa gaaaaggtat tatcagagca actagtccca
    14821 ggagatattg ttcttattga agaaggagat tgtatttcag cagatggtcg cttgattaaa
    14881 acaacagatt tgcaagtaaa ccaatcagct ttgacagggg aatcaaatcc tatttataaa
    14941 gataacaatg ttgaaaacta ccagtctaaa acattgattg aatgtgataa tatggttttc
    15001 gcaggaacga cagtctccag tggtagcgca acaatggttg ttacggcaat tggaatgcaa
    15061 acccaatttg gtcaaatcgc tgatttaaca caagggatga aatcagaaaa aagccctctt
    15121 caaagagaac ttgatcggtt gaccaaacag atttctatca tttcaattac tgtaggaatt
    15181 atctttttct tagcagcgac tttttttgtt aaggagccag tgtcaaaatc atttatcttt
    15241 gccttgggga tgatcgttgc atttatccca gaaggtttac tacctaccgt tacactttct
    15301 ctagctatgg cagtccaacg aatggctaaa gaacacgcac ttgtcaaaaa actttcttca
    15361 gttgagactc ttggagcaac ctcagttatc tgttctgata agactggtac attaacgcaa
    15421 aatgaaatga ccgtcaatca tttgtggcaa aatggaaaga gttaccaagt gacaggactg
    15481 ggttatgctc cagaaggaca gattcttttt gaaggtgaca atatttgctt tggtaatagt
    15541 gatagaggag acttagaaaa acttattcgt tttgctcatc tctgtagcaa cgctcaagta
    15601 ttaccaccaa atgatgatcg ttcaacttat actgtacttg gagatccgac agaagcttgt
    15661 ttgaatgttt tattggaaaa atcagggata aacatacaag aaaatcgtaa gtttgcacca
    15721 cgtttgaaag agttaccttt tgattcggtt agaaaacgca tgacaactat tcatagctta
    15781 ggaggcgatg aaaaggataa aaaaatttca atcacaaaag gtgcgccgaa agaaatactt
    15841 gatttatcag attatgtctt atctgatggc aaagttatcc ccttaaataa ggaagaacgt
    15901 aataaaatac agttggctaa tgatacattt gctaaagatg gacttcgcgt cttagctgtt
    15961 tcatactgcg atattgaagg cttttcaaaa gaacaatgga cgcaagaaaa tttggaacaa
    16021 cacatggtgt ttatagggtt gatagcaatg agtgatccac ctcgtgaagg cgttcgtgaa
    16081 gctattgata aatgtcatgc agcaagcatt cgtattatta tggtgactgg agattacgga
    16141 ttaacggcac tgagtatcgc taaaaatatt ggtattattc gtaacgatga tgctaaagta
    16201 atttcaggtt tagagttatc agaaatgacg gatagccaat tgaaaaaaga attgtcaggt
    16261 gaagtcgttt ttgctcgtgt agcaccagaa caaaaatatc gagtagtaac gatattacag
    16321 gagatgggtg aagtagtagc agttacagga gacggggtca atgatgcgcc tgcgctaaaa
    16381 aaatccgata ttggtgtagc catgggtgtt accggtactg atgttgctaa agaatcagct
    16441 gatatgattc tgacagatga tcattttgct tctattgtac atgccgtgga agaagggcgg
    16501 gctgtttatc aaaacattaa gaaattcttg acgtatattt ttaatagtaa tactcctgag
    16561 gcggttccgt cggcattctt ccttttttct aaaggtttta ttcctttacc attgactgta
    16621 atgcaaatac ttgctgttga tttgggaacc gatatgcttc ccgctttagg tctaggcgtg
    16681 gaaccaccag aaacagatgt tatgaatcgt cctccaagac ggctaacaga tcgcttattg
    16741 gacaaagggc tacttataaa atctttcctt tggtacggca ctattgaatc cgtattagct
    16801 atgggtggtt ttttctgggc tcattactta cgttatggta attttacttt tttcgtcgca
    16861 aacggcatac cgtacagaga agcgacaact atgacacttg gagctattat ctttagccag
    16921 ataggtatgg ttatgaatag cagaacgagc tatcaatcta ttaaaacact atctattttt
    16981 ggtaataaat taattaattt tggtataata atggagattt tagcattttt agtcttagta
    17041 tacgtgccac tattccataa cttat
//
LOCUS       DATRNX010000039        16845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRNX010000039 DATRNX010000000
VERSION     DATRNX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(259..873)
                     /locus_tag="VBC81_001025"
     CDS             complement(259..873)
                     /locus_tag="VBC81_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6912074.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(1006..1431)
                     /locus_tag="VBC81_001026"
     CDS             complement(1006..1431)
                     /locus_tag="VBC81_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6912075.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(1558..5964)
                     /locus_tag="VBC81_001027"
     CDS             complement(1558..5964)
                     /locus_tag="VBC81_001027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6912076.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(6087..6671)
                     /locus_tag="VBC81_001028"
     CDS             complement(6087..6671)
                     /locus_tag="VBC81_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6912077.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(6796..8649)
                     /locus_tag="VBC81_001029"
     CDS             complement(6796..8649)
                     /locus_tag="VBC81_001029"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6912078.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(8741..10000)
                     /gene="rseP"
                     /locus_tag="VBC81_001030"
     CDS             complement(8741..10000)
                     /gene="rseP"
                     /locus_tag="VBC81_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6912079.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(10031..10825)
                     /locus_tag="VBC81_001031"
     CDS             complement(10031..10825)
                     /locus_tag="VBC81_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6912080.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(10840..11592)
                     /locus_tag="VBC81_001032"
     CDS             complement(10840..11592)
                     /locus_tag="VBC81_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6912081.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(11759..12100)
                     /gene="yajC"
                     /locus_tag="VBC81_001033"
     CDS             complement(11759..12100)
                     /gene="yajC"
                     /locus_tag="VBC81_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6912082.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(12177..12521)
                     /locus_tag="VBC81_001034"
     CDS             complement(12177..12521)
                     /locus_tag="VBC81_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6912083.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(12712..13875)
                     /locus_tag="VBC81_001035"
     CDS             complement(12712..13875)
                     /locus_tag="VBC81_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6912084.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(13900..15237)
                     /locus_tag="VBC81_001036"
     CDS             complement(13900..15237)
                     /locus_tag="VBC81_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6912085.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            15391..>16845
                     /locus_tag="VBC81_001037"
     CDS             15391..>16845
                     /locus_tag="VBC81_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6912086.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRA"
BASE COUNT         5446 a         3475 c         2633 g         5291 t
ORIGIN      
        1 ttgaatgaaa cattctattc acaattaaaa gaaggagaaa tgaaactaag tgaaaatccc
       61 gaaaaaggag aaacgaacac gaattaacct ttcaaatatc tacatcaaat aacagaagtc
      121 tccgtatcaa gcgaattgta gcttttttga atatactaaa aacactccta cctctatttt
      181 actagaagta ggagtgtttt tttcatttta gttcatagat gcagtgccat tgctatcctt
      241 gccttttttt gtacagtatt atccgatgac tttaaagcgg tcatcatcat tcatgaattc
      301 tttttctcct gtttcagctt cgattgaacc aaaatttagt tgtccacgaa gtttccaact
      361 agctggaaca ccataaacag cttgtactga tgcatcaata actggattgt aatgttgtaa
      421 attaccacca agtccaagtt ctgcagaaag ggctgtccaa gtatttactg aggcaatacc
      481 tgttgattgc tcagaccaaa ctggaaagtt atccgcataa agaacgaatt gttcttgaag
      541 agatttgaca acatcttgat cttcaaagaa gagtactgtt ccctttgaag ctccaaaact
      601 agctaatttt tccttagttc cagcaatagc tgtctctgga gcaccttgta ctttcattgt
      661 ttctaccaaa tcattagcaa ccaattcatc ccagaactta gtaacttcat cattcaataa
      721 aatgacaaca cgggaagttt gactattaaa agcagatggg gattggcgaa ctgcctcttt
      781 aataatctct actattttct catcagaaac ttctgtattg cgaccaagag cgtaaataga
      841 gcgacggttt tttaattctt ccaaaaatga catatttttt ctccttgttc ttcaatctca
      901 gatatcttac tacatttgtt ctagtgacat atgaattaga ttaaagttat ttattactat
      961 gtagtaatta tattaccact atatcgtaaa gtcaaagaat gtgactcaca aggttttaaa
     1021 tttttttaaa aggttaatca agtcttcttg ctctgtttct gttaagactg aaaaagcatc
     1081 ttctacacgt ttaatatgct ctggtagagc tttttttatc acttcttctc cttcagttgt
     1141 taaactcacc aaaaaggctc tcttatcatt cggacaagaa tggcgtaata cccatccctt
     1201 cttttccata tttttaataa caacagtcat attacctgat gtagcaagca tattttcaat
     1261 caattctcca attttcattg tgcctttagc atagagaaca tccaaaactg caaactgagt
     1321 tggtgttaat tcaaattctt taaatgattc tgagactttt ccgtctattg ttctaaatgc
     1381 ttttcgcatg acaaccatag atttaaccgc tgaattttta aagtttccca tttcatctcc
     1441 caaacgtatt ttttcttatt ttatcactaa ttagttattt taagcaagaa tatcatcttt
     1501 gccctatcaa aagcagctgg caaataataa ggttgagtaa ctactcaacc ttattgctta
     1561 gaagaaatca tcaaaaagac tcaactgatt gtcttctggt aaatttccca aaatacccat
     1621 ctcatcaaat ttttcaacta aggtacttga gactcctcct cgtttacgaa gctctgtttt
     1681 tgataaaaat tcaccatctt cacgtgctcg aactatctgt tttgcaacgt tttctcccaa
     1741 gccttccata gcaacaaatg gagggattag cgtatcctct tcaataataa agtcagtcgc
     1801 atggctacga taaagatcta atttcccaaa tttaaagcca cgctcaagca tttcattaac
     1861 caactctaat gtcgtaaaaa gatcattttc gacattcgtc gcctcattac gctgacgctt
     1921 ctcagtgata tccttcatgc gcgccttaac ggcatcaagt ccagcactca ttgtgcgtaa
     1981 ttcaaaagct ttggcgcgaa tagaaaaata agcacaataa taaaatattg gataatgtac
     2041 tttaaagtac gccacacgta aggccattaa aacataggct gctgcatggg ctttagggaa
     2101 catatacttg atttttccac acgattcaat ataccaatcc gggacattat tatcacgcat
     2161 tgcttggata taaccgttac gctcatcttc tgaaattttc aaccataacc ccttacgcac
     2221 gcgctccatg attgtaaagg ccatctttgg ttgtagacca gcgtgcatta aataaaccat
     2281 gatgtcatca cgacaaccaa taacggttga taaggtagca ataccttctt tgattaagtc
     2341 ttgagcatta cctaaccaaa catccgtacc atgtgatagt cctgacagtt gcaacaattc
     2401 tgcaaatgtg gttggatggg tttcgttcac cataccacga acaaagttcg taccaaactc
     2461 aggaatacca agcatccctg taggagttcc aatttgctct tctgtcactc ctaaaacttc
     2521 cgtaccagaa aataacttca taacgtctgg atcatctgga aggatatttg atggatcaat
     2581 tcctgataaa tcctgaagtt tacgaatcat agtaggatca tcatgtccta gaatatctaa
     2641 cttcaagaca ttctcatcaa tatcatggaa attgaagtga gttgtctgcc aagcagctgt
     2701 catatcatct gctggatatt gaaccggagt aaaatcataa acatccatat agttaggaat
     2761 aacaacgata ccaccggggt gttgacctgt agaacgtttg actcccgcag caccagtcgc
     2821 taaccgttcg acctctgcat cgttataaaa tttattgtaa tctcgttcgt aacctttgac
     2881 aaaaccaaag gcagtttttt cagctacggt acctaccgtt cctgcacgaa aagcatattc
     2941 ttctccaaag atatcacgaa catctaagtg agcagaaggt tgatcatctc cagagaaatt
     3001 tagatcaata tcaggaacct tatctccatc aaatcctaag aaagtttcaa acggaatatc
     3061 ctgtccatct ttcttgtaca aagtaccaca tttaggacaa tttttatttg gtaaatcgta
     3121 acctgaacca caagaaccat ctgtgataaa ttcagaatgc tgacaatttg ggcaaacata
     3181 atgaggtggc attggattaa cttcagtaat tcctatcatg gtcgctacaa aactcgaccc
     3241 aacagaccca cgtgatccta ctagataacc acgttcatta gaacgttgca ccaacatttg
     3301 cgaagcaaga tagataacgg caaatccatt accaagtata gatgctaact ctttttcgat
     3361 acgtaaatca ataatatcag gaagaggatt accatatatt tcgaatgctt tagcataagt
     3421 taattcagca actcgttctt cagcacgatc aacaaacggg gtatagagat ccccttttac
     3481 aacttcaacg tcttcaaatc gatctgcaaa agtattcgta ttggttacaa cgatctcata
     3541 ggctaaatct ttaccaagaa aggcaaattc atctaacatc tcatttgttg tacggaaatg
     3601 tgcttttggc aaaggcgctg gttgagcatc ctccccacga ccgatagtcc tattaatcat
     3661 ggcaccttgt ccaagactac gaacaataat ctcacggtaa atctcatctt caggctcaat
     3721 ataatgaaca tttcccgtag ctaaaacagg cttatctaaa cgccgaccga cctctattag
     3781 gtctctaata atttgttgaa tccccgcctc atctttaatt aaatcacgaa ccaccaaggg
     3841 gcgataaata gcaggtggca tgacttcaat aaaatcataa tatttagcta gggtcactgc
     3901 tgcatcaatt ccatttgata acaaggcatc aaaaacttct ccatcagaac aggcagtacc
     3961 tagcaagaga ccttctctgt gagcatctaa aactgatcta ggaattcttg ccaccccttc
     4021 aaaatattta acatttgaaa ggctaactaa cttaaatata tttttaagtc caacctgatt
     4081 ttggacataa atggttgcat gctttatgcg tgcctttttg taagagtctt cagctaccaa
     4141 tttagtattg agctccatca aattagtcac atcacgattt tcccgcgcct cttttaagaa
     4201 aataaagagt aagcgacccg ttgcttcagc atcgtagttg gccatatgat gatgttccaa
     4261 agcaacttgg aaacgtttgg ttagaggccc caaaccatga cgcttatatt caggataaag
     4321 atttcttgca aattctaagg tatcaatgac cggctgtgtg atcaaaggca gattatgacg
     4381 ttcataattc gcattcataa aaccaacgtc aaaggttgca ttatgcgcaa ctaaaaccgt
     4441 accttgacaa aaattctgaa attcctgaag gacttgtaaa ataggttttg aacctctgac
     4501 atgattatcc gtaatacctg tcaactcagt tgtaaagtca gaaagaggat ggcctgggtc
     4561 aataaactca tcaaattgct caataatatt acccttgaac atttttgatg ctgcaatctg
     4621 aattaggtca ttattagctg cagaaagacc tgtagtttcc acatcaaata caacataagt
     4681 tgcttcgtgc aaattcatat caacttcgtt gtaggaaata ggcactttat cttcaacaat
     4741 atttgcctct aatccaaaaa ttgcttttat acctgctttt tttgctctgt ggtagccgtg
     4801 cggaaaactt tgaacattgg catggtcagt aattgcaata gctgggtgtc cccacttagc
     4861 tgcagtatca atgaggctct cgacagttgg taaagcatcc atcgttgaca tattggtatg
     4921 agcatgaaat tccacacgtt tttgatctgc tggcattaag tctttacgtt catgatgcac
     4981 aatctctttg atatcttgaa cattcattgt caaagattta gtaaagttat tgttctcaat
     5041 attccctctg acccgcaacc acgaaccttt agaaatcata tcgtacttct tcaattcttc
     5101 atcatcttta gcccactttt gcatagcaaa acttgaggta taatcagtca tcttaaaatt
     5161 aatgatatga cgtccagtgc gggttgtttt tcgttcaaca ctaaaaacca ttccttcaaa
     5221 aacgatacga ttttcttctg tggtcacctc aatcatgggt gtaatctctg ctttttcaaa
     5281 gccagcttga cgttgtttga tacgctcttt gaaatcataa ttttgagttg gtgtcacttc
     5341 ttctgatgga ggcgctgaat cttctagtga tttttgtgct tccaaggctt gcatagcctc
     5401 ttggttagct ttttcaagca attgctcgcg gttagtttca aatgatgatt ttaagttttg
     5461 cgtcatttgt tcatcagaaa ccatatcaat agcaagttta ccaaatccaa acaatgaaaa
     5521 ttgttgttcc aagcgcggca aatgattctg acgaaaatga ttattattga caaattgagg
     5581 tgctgaaata atcatctgac tgccattgta atgaactttt aattgtgaaa aagaactctt
     5641 aaaacttgca ctattacaca agggttcaca aaaagcttgt tgataataat cctgtagcag
     5701 atcatcagaa aaatcaatag tttctgctct tatatcaaaa gtagccttta tatctgcttt
     5761 ttcaaaacta ttcactaaac gagtttgtaa ttcacaatac acctcaattg gcaataattc
     5821 tggaaatgaa aaatgaaatt cccataagcg tgatagagaa tgaaccttta cctcgataat
     5881 atcagcagac gaaaaaacac ttgaattctt aatttcaagc ggcatttcaa tctgatccat
     5941 aagtttcttg aacaattctg acatagtttt ctcctgaata tcttacttcc attttatcac
     6001 atatctgtaa atatttcgag ataacaaaag gaggctgtta aaaaaggcat ttattagcct
     6061 tcttaacaac actctcttaa ctgagactat ttatcataag catctagact gtaggtttca
     6121 ataatttggt ttaatttact agcataagca gtatctgtcg cataaagacc tgttagagct
     6181 gcagtagcat ccttataaga agaagtattt gatttccaaa cagatgcata cttttgacta
     6241 gatactaact cagcataatc atatagtgaa gcagaataat ttggataggc gcgaaaagga
     6301 gcttggattt gagtcatatt gcctttccca tcatcttcta aagtaggcat ttggacagat
     6361 ttacctttat attctccttt gatgccaaag aggttataat taggagcctt agacaaatct
     6421 gattgtccac tggatgattc caaaatagct tgtgctaata agactgacgc atacaaatca
     6481 taggtcttag aaatttcaat agctgttgga gaaatcgttt gaataaaagt cgtcgtttga
     6541 tccgatgcac tagtgactgt cggttcgaca ttatcatttt tataattgta aaaccaaacc
     6601 ccacccaaac caaaaactaa tagtgttgtt gttaacctca ataccaattt atcttttttt
     6661 cgacttttca tgatggctcc tttctaaaat atcatttcat tataacaaaa ggcagttgct
     6721 ttttaccaat aagaaccata ctaaatttca ctccatataa aaaagttagc acatcagact
     6781 aactttttta tcggcttatt tttttgttag aatctctaaa gtttcaatta agttatcagc
     6841 gtgaacctca atcgtatcac cacttgcttt aattttcact tcaacgatac cttcagaagc
     6901 tttcttccca acagttactc gaattggaag tccaattaag tcactgtctg agaatttaga
     6961 accaacacgt tcattacgat cgtctgtcag gacttcataa cctttaagca tcaaatcagc
     7021 ttcaattttc tcagtaagat cttgtgactc ttgatctttg acattgacag taatcaaatg
     7081 tacatcaaat ggggctaatt cttctgggaa attaatcccc caagcaaagc gataagctcc
     7141 ttttggtgtc ttattcacaa aaagtcgggc atgttgttca atgacagctg agagaatacg
     7201 actaacacca ataccataac accccataac aataggattt gagcggccat tttcatcaag
     7261 gatgtttgct cccatactat ctgaataacg ggtacctagt ttaaagatat gtccaatttc
     7321 aataccacga gcaaatttca gagtaccttt accgtctgga gaaatctccc cttcttttac
     7381 ttcacgaata tcaacgtatt cagctgtgaa atcacgttct ggattaactc cagtaaaatg
     7441 ataaccatct tggttagcac cagacacagc attagcaagg tcttgaactt tacgatcagc
     7501 aataatttta acagactcag gtaggttaac tggcccaaga gaaccaaaac ctgcaccaaa
     7561 aatctctttt gcttgctctt cacttgcagg ctctaagaaa tcagccgcta aataattttt
     7621 taacttcaca tcattgactt gatcgttacc aactagtaaa gcaaccacgg gctgttcatc
     7681 agcaataaac aatagtgtct tgatagtttg attttcatca attgataaaa atccagctac
     7741 ctcatcaatt gatttgcagt taggagtttc tacttttgtt acttcttcaa aggtagcagc
     7801 cttagtagat ggtttgtact cattcgttgc catctcaagg ttagcagcat agcttgattc
     7861 agtggagtat gcaatagtgt cctcaccaga aactagccaa gccgagagtt ctgccttaat
     7921 ttcttctaaa acatcctctg gaatatcatc aatagaagga atagttttat caaggactaa
     7981 ccaacgattt aaatctgtac gattgggagt tactgccata aattcttggc tatctttacc
     8041 tcccatagca cctccgtcac ctataatgcc tttaaagtca agtccagcac gtgtgaagat
     8101 ggcttcatat gctttacggt aatcttcgta agtgacatca aggtcttcat aatctttgtg
     8161 gaagctgtat ccatctttca tgataaattc acgtgttcga agaagtccat tacgaggacg
     8221 cttctcatca cgatatttag actgaatttg ataaaggttt agtggtaatt gcttataaga
     8281 ttttactgca tcacgaacaa gagttgtgaa agtctcttca tgagttggtc ctaagataaa
     8341 atccgattga tcacgattct ttaatttata aaggtcttca ccatatgttt cgtagcgccc
     8401 agattcacgc cataaatctg ctgtaagaag agctggagct aacatttcaa ctgcaccaat
     8461 cttctcaaat tcttgacgca taattgtttt aaatttttca atagtacggt tagctagagg
     8521 caaataagca tagattccag ccgaaacttg gcggacatat cctgcacgaa ccataagagc
     8581 atggctgata acctgagcat cacttggcat ttcgcgaagt gttggaataa gcattttact
     8641 ttgtttcatt acaataagat acgactaatc aaagttgatt ctttttcaat tgagaattgc
     8701 aattgaaaaa ttcgtatttc ctttcacgtt ttattatctt ttagaagaaa gctcgcatga
     8761 tatcgttcca ggttacagca atcattaata ctaacataac tgctacccca gcgagcgtaa
     8821 tataagtttc tgtttcttgt ttaagtggtt tgcgacgaat ggcttctaaa atattcatca
     8881 ctatcttacc cccgtcaagg gctggtatag ggatgagatt cataatacct aagttgatag
     8941 agattaatcc cattaagttg agaactgtga cgaaaccatt tttagcagct tggctactag
     9001 cctgatagag agctactgga cctcccaatt tattaatact aaaatgagca ataagtttct
     9061 ttaattcatt taaaattcta aagaaacttt cccatgctag tttaaatcca cctaaaagct
     9121 tgtctttaaa gcttgtcttt agagctggca taatgccgat gatgtaggat ttatcaactt
     9181 tctgtggctt aacgttgatc gttttcacca cctctttgct cttaatttta agggcaagtt
     9241 tttggttttt ttctaaatga ctagttgact tttctactgc tgctgtcaac tgttcccagt
     9301 tagaaacttt gtgcgaccct atttgtaaaa tacggtcatt attttttaaa cctgcactag
     9361 cagcaggacc attttctgat acacggacct gattggttga taaatcttga acacctcctt
     9421 gaataaaagc aagtgcaata aagactacaa gacctaaaat aaaattattc atcggacctg
     9481 caaaattggt aattaagcgc ccccatacag aagcattctg atactgcaca tctaagggag
     9541 caatacgaat ttcagtacca tcctcctcaa tgatagtagc atcgtgatca acactatagg
     9601 tttttgtctc acttaagacc aatcctgtga tagttagttt atcttctaaa tcataagctg
     9661 ttacattgat tggtaaagaa gtattgtcta attgttttcc tgataaatta atacgagtta
     9721 caatgccttc tttatttaaa gtaaggctag ccggcgtccc tgtcttaatt tcagtcttat
     9781 catcacccca accagccata cggacataac cacctagcgg taatatacga atagtatagg
     9841 ttgttccttc tttatcaatg tgagaaaaga tttttggacc cataccaata gcaaattcac
     9901 gaacaagaat tcctgatttc tttgcaaaat agaaatgccc aaattcgtga acaactacaa
     9961 tgacaccaaa aataataata aaggttaaaa ttcctagcat tcaattcctc tctaaataca
    10021 atatattatt ttagaataag ccaaagaagt gcatgattgg gaaaacaaaa atcatactat
    10081 caaagcgatc caaaatcccc ccatgacccg gaatcaattt acccgaatct ttaacaccaa
    10141 aatgacgttt aatactactt tcaactaaat ctccaaattg accaaaaatg ctaaagatag
    10201 ccactaaaac taacatgact aggaagctat gaggcgcata gacggtctta tcaaaaagca
    10261 taaaaaagaa agctaccaca atggctgaag caattcctcc cagactccct tcaattgttt
    10321 tatttggaga cactgaaggt agtagtttgc gttgtccaaa ttgacgacca atcatataag
    10381 caccgatatc agtcgcccag acaataaata aagctaacaa caccttatct attccagcca
    10441 tacgagcact gactagattt tgaaagccaa tacccacata aaaactagac gcaattggaa
    10501 aggcagcatc ttcaaaactg taagaattac tgttcaaaac agtacctgcc aaaatcataa
    10561 aaataacaat accgtatgct gataaactag cgtcaactgg taaaaaggac aaataactat
    10621 ctaaaggaac tgttaaaaca aaggctccta tcatagctaa tgcaccttca aaggaaaata
    10681 tttctaaacg acgcatacga agtaattcag aaacaccaat catagctaaa agtccaacta
    10741 aaaattggaa aggcaaacca cccattacta aaaaagggat aaatatggca agcgcgacag
    10801 ctccccaaat aacacgttct ttcattttct ctcttctttc tatacactac caaatcgacg
    10861 atggcgttga ttataatcca caatggcctt atgtagttcg tcttttttaa aatctggcca
    10921 taaaacggga gtaaagtaaa attcactata agctgattgc caaggtaaaa agttactcaa
    10981 gcgtaactct ccactagtgc ggataatcaa atcagggtcc ctataaagat agggtaagct
    11041 atttgtcatt aaatgatttg ctactaaatc ctctgtaata tcgtcaggat tcaacttagc
    11101 ttccaaaaca tcctgagcaa tttccttaat ggcattagtg atttctgaac gtccgccata
    11161 atttagcgca aaattcaata cgagccccga attatgtttt gtcctcaaac aagcgcgttg
    11221 cattgcatcg tatgttgcct taggtaattt atgagtatct ccgatgacct gtacacgtac
    11281 attattttta tctaactctg gtacatattt atcaaaaaat tcaaccggaa ggttcataat
    11341 aaacttaact tcatcatctg gacgtgacca attttctgta gaaaaggcat agacagttag
    11401 tactttaaca cctaattctg aagctgctat tgtaacttct tgaagagcat ccatccccgc
    11461 cttatgtccc ataacgcgtg gttttaaacg tttttttgcc caacgtccgt taccatccat
    11521 gataatacca atatgtgctg gtattttctc caaaggcatt tctacttgtt tttttctttt
    11581 aaatataaac ataatgttcc ttaatttagc aatcgttttt cttatcagcc cttttaaaag
    11641 agctggaaat atcccatact ataacatttt accacatttt aacttatttt tgagtattaa
    11701 aatgccattt gtgacagcaa actaaaaaga ccagaacaat catctggtcc tacatatctt
    11761 attcagtttc gatggctgtt tctttatttt cgtcttcatt gacttcaata atcttaacat
    11821 cttcaactgg ttctgttggt gttgctgcct tagagacaac agatttgata gctgccaact
    11881 caaacgtcaa gtagacacca tcaacatcta aaaccattcg ttgtgcttca gtattaactt
    11941 catcaactac tccaaaaaga ccaccaattg tgacaatttc atcacctttt tgaacagcat
    12001 tcaattgttt ttgacgttct tgtgcttgtt tcttttgctg acgttgcatg aagaacatca
    12061 taccaaccat aacaacaagc ataataatag gtaagctcat ttgattctcc tttccgtgtt
    12121 ctacgtaatc attactatag cagatttatg ctatatctgc aaggagatac tgtctcttag
    12181 ccaataaatt cacggatttc ttcttttgtc atttttgaat cgcacttcac cttgatagtt
    12241 ccgtttttaa caaccatcaa acctggcact gtaggaatgt catatttatt tcgaaaatct
    12301 ttatttgcag tccaatgatc aaaatcttca ctatctaaat ggaataattc aaattcatct
    12361 tcatcagcta cttctacaat tttaggaaca aagaggtgac aaaatggaca agaaatacga
    12421 ccaatgaaca gtacaaaatc ttgatttgaa tcaattttat cttcaatttc ggcagccgtt
    12481 atcaaagtca tggttgccaa aagttcttca aaagaataca tagttaacca actttctaaa
    12541 tcttatattt aaagaataac ctatgatggc aaaacttgca aggcaagagt aacaacgatt
    12601 ctatcaaaat cattaactca cttctcgctt caaactatcc tcaaaaatgc tcactcatat
    12661 ttaaatgact catcaaaaaa actgagcata gctcagtttt tattttacac cttatttttg
    12721 tacagcatta ctaacgcttt tagcaactac atcggccaca tcattttgaa aagcatttgg
    12781 aatgatgtga gttgtgctta attcttcctc cggtattaaa ctagctatgc ctctcgctgc
    12841 cgcaatttgc atttcaacag ttatcgtttt tgctctagca tctaatgctc ctctgaaaat
    12901 acccggaaat gctaaaacat tattaatttg atttggaaaa tcactacgac cagttccaac
    12961 tatgtaagct ccggctttta aggcttgatc tggataaatt tctggaatag gattcgccat
    13021 tgcaaataca ataggtttat cagccatctt ggaaatccat tctgcatgta atgcttccgg
    13081 agcagaaaca cctataaaaa catcggcatt ctccaaagca tcttctaagg atccagattg
    13141 gaattctcta ttagtcaatt ttgcaatcgc tttgtgatga ggggctaatg actcccgatc
    13201 tttatcgtta ataatgccaa atcgatcaac taccgttaca tgttttgctc ctgctgatag
    13261 aagcttcctt gttatagata agcccgctga acctccacca ttaacaacta ctttgatatc
    13321 ttcaatgtct tttttaacta acttcaaact gttaaacaaa gccgctaata ctacgattgc
    13381 tgtaccatgt tggtcatcat ggaaaactgg tatatcacat tcttcaatta ggcgttgttc
    13441 aatttcgaaa caacgtggtg ctgaaatatc ctctaaatta atccctccaa aagtcggcga
    13501 aatcgctttt actatagaaa taatttcctc tgtatcattt gttttcaaca caattggcac
    13561 agcatctaca tttgcaaaac gtttaaagag tgctgcttta ccttccatga ctggcattgc
    13621 tgcctcagga ccaatatctc ctaatccaag aacagctgaa ccatcactta ctacggctac
    13681 tgtatttttt ttagtcgtca actcatatgc aagctccaca tcctctgcaa tagccgatga
    13741 tactgacgcc acccctgggg tataggcaat actaagatcc ctcttatctt ccaccttaac
    13801 ttttgaaatt acttctaatt ttccaccatt ttctctagct tggttgatag ctaattgacc
    13861 taaattttca ctcattttga taactccttt tgataaatat tagaataaca tgcgtaaaat
    13921 ggctgtcatc gtgataacag taatagctcc tcctaaacgc gtagcaactt gagcaaatgg
    13981 catcaagttc attcggtctg ctgtacttaa aatggcaaca tctcctgttc ctcccatacc
    14041 actttgacaa gctgaaataa tacctgcttc aacaggattc atattaagga atcgcgatac
    14101 aaagaatcca acagaaataa ctgtaaatac aacactaata acaactatga aatattgtat
    14161 gctaagcgtt gccacaacat cttttaacgg gatataaagc aatcctagcc ctgccattag
    14221 aggaaatgta aaattaccag aaataaattt ataaagttgc ttagctccat tttgtgtttc
    14281 tctaggaata acattgatat atttcaaaat agctgctgcg ataatcatta atacgggacc
    14341 aggaaatcca gttaattgct gcaacaaatg tccaaaaata aagagactac atgctgttaa
    14401 aacacctcca cccattttct taacgtcaat tgggccagaa tgatcagcaa taatatctga
    14461 catgtcctct cctttattta accttactaa ttgcccttga ccagacaagt gtggtttctt
    14521 ttctcccaaa cgattcaata gtgcagtaca taaaatggca aagaaattac caataatagt
    14581 ggctgggatg agttgagcaa ctagttgttc gctagctaca ccggtaattg aactatagcc
    14641 taacgataat ggtaaaatac cttcaccaat accaccagct aaaacgggtg ttacgatata
    14701 aaacaaagtg tgtcgccact ctaagcctaa aataacaccg acaaatgtcc ctaccatcat
    14761 cgcacaaacc attcctaata acatggggaa aatcattctt agtaatccct gaatcaacat
    14821 tttccgattc atccctaaaa tactaccgga cactaaacaa gcaatataaa aataaagaaa
    14881 gttagcttgc ttcatcaaca tatttgttga ctctaaaaca tttttattaa tgaggttgaa
    14941 aaacaccaaa atagatggta ctaataaaga taaaatagct gggccaccga agtttttaaa
    15001 accagggatg ctagctccaa tagtacctaa gaaccatccc attgttaaga tgactgcaaa
    15061 tcctcctagc atattgactg gcaatttttg taaaaagcct gctagaagaa tcaataatgc
    15121 taaacaaaca tatactggta agggaactga gcctacctta atatgaaata gtttagtctt
    15181 taagtcttgc ttttgacctc tagaaccctc attattcact accttcacgt ctgccatgaa
    15241 agcacctcct tttacttctt atgtacagtt tactttggga cttagtgata aggaagttta
    15301 tttaagtaat tgaattattt caacttattt aattaattta agctggattc tagtttttag
    15361 gctatgttat actaaactcg gaggatgctg atgaaaaaaa aactatctct ttgggcattt
    15421 ctatcattga ttttagtcac tatgaccatt tgtatatttt ctatttttta ttatgtcact
    15481 atacatcagt cctatcgtat ggtaagggta caggaagaaa aaatcttaaa aaatactggt
    15541 tatgctttat cgagaaatcc acaagttata caaacactca aagataatca ctataatcaa
    15601 agtctccaaa aacaaatgct attccttagc aaaaaatcaa atttagacta tattgtcctt
    15661 ataaatttaa aagggatccg ttttacccac cctgactcca ctaagattgg taaaccattt
    15721 caaggcggtg atgaacaagc tgtttttaag ggaaaagcta ttatgtctac tgcagaaggt
    15781 tctttaggaa aatctttacg atacttaatt ccagtatatg atcaccaaaa acaggttggg
    15841 gcaattgcag ttggcttaaa attaacgaca cttggagact tatctcaaag cagcataaaa
    15901 gagtttagta agcctctctt gattagtatt ttgattagct tagtcgtcac aagtataatt
    15961 tcttatggct taaaaaaaca acttcacaac ctccatccaa gtgatatttt tcaacattta
    16021 gaagagcgca atgcaacact ggaccaaatt caagccgctg tatttgttat tgaccagagg
    16081 catatcatca aaagaaataa tcctgcagct agtttattat tcaaaaaaga ggggcaacga
    16141 gacctatttt caggtaaatt attagaatct ttaattcctc aactcaagca ggatcatttt
    16201 tcaaaaaaaa ctgaacaagt attacatttt caaggacaag actatttact atctatctcg
    16261 ccaattacgg ttaaaacaca aaatagaggc tatgttgtct tcttaaggaa tgttactgaa
    16321 acacttttca ctttggatca attagcacac actacagctt atgcttctgc tctacaggcc
    16381 caaacacatc aatttatgaa tcaacttcat gttatttatg gacttgcaga catcgaatac
    16441 tacgacgagt tgaaaattta tttaaaagaa ttattggagc cacaaaatga atttttagca
    16501 cgcttatcaa tgttagttag agagcctcga ctggctagtt tcattattgg cgaaagagaa
    16561 aaattcgcag aaaaacacat caatctatcc acagaaattt tagtagaaat ccctaccaag
    16621 tcaactgttg aggatgttaa taactatctc ttgctccaca gatatattaa tacgaagata
    16681 ctcacgctat taaattcaac gactttagtt tctttacgtt taaattatca aaataacctc
    16741 attgaaacag attatcaatg ggagaatgag aagtggttac tcaatgacta ccatcagtat
    16801 ttcaacgacg cttattttca acagttactt gtagattcaa gagct
//
LOCUS       DATRNX010000040        16694 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRNX010000040 DATRNX010000000
VERSION     DATRNX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16694
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            67..675
                     /locus_tag="VBC81_001038"
     CDS             67..675
                     /locus_tag="VBC81_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6912087.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRD
                     ADNKSQVGQLIGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNN
                     SRTISVINKLPKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
     gene            883..1959
                     /locus_tag="VBC81_001039"
     CDS             883..1959
                     /locus_tag="VBC81_001039"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6912088.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2129..2467
                     /locus_tag="VBC81_001040"
     CDS             2129..2467
                     /locus_tag="VBC81_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912089.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2598..4139
                     /locus_tag="VBC81_001041"
     CDS             2598..4139
                     /locus_tag="VBC81_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6912090.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4179..5411
                     /locus_tag="VBC81_001042"
     CDS             4179..5411
                     /locus_tag="VBC81_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6912091.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5624..6325
                     /gene="pyrF"
                     /locus_tag="VBC81_001043"
     CDS             5624..6325
                     /gene="pyrF"
                     /locus_tag="VBC81_001043"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6912092.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6338..6967
                     /locus_tag="VBC81_001044"
     CDS             6338..6967
                     /locus_tag="VBC81_001044"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6912093.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            6979..8271
                     /locus_tag="VBC81_001045"
     CDS             6979..8271
                     /locus_tag="VBC81_001045"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6912094.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8436..9359
                     /locus_tag="VBC81_001046"
     CDS             8436..9359
                     /locus_tag="VBC81_001046"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6912095.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9373..10449
                     /gene="carA"
                     /locus_tag="VBC81_001047"
     CDS             9373..10449
                     /gene="carA"
                     /locus_tag="VBC81_001047"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6912096.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10480..13662
                     /gene="carB"
                     /locus_tag="VBC81_001048"
     CDS             10480..13662
                     /gene="carB"
                     /locus_tag="VBC81_001048"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6912097.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            13788..14111
                     /locus_tag="VBC81_001049"
     CDS             13788..14111
                     /locus_tag="VBC81_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912098.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            14108..14578
                     /locus_tag="VBC81_001050"
     CDS             14108..14578
                     /locus_tag="VBC81_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6912099.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            14556..>16694
                     /locus_tag="VBC81_001051"
     CDS             14556..>16694
                     /locus_tag="VBC81_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO6912100.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHDLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSW"
BASE COUNT         5662 a         2690 c         3243 g         5099 t
ORIGIN      
        1 tataatctaa ataaaaataa taataaaatg acttttaaaa tttaaaaaaa gtaaggagaa
       61 aattaattgt tcaataaaat aggttttaga acttggaaat caggaaagct ttggctttat
      121 atgggagtgc taggatcaac tattatttta ggatcaagtc ctgtatctgc tatggatagt
      181 gttggaaatc aaagtcaagg taatgtttta gagcgtcgcc aacgtgatgc ggaaaacaag
      241 agccaaggca atgttttaga gcgtcgtcaa cgcgatgttg agaataagag ccaaggcaat
      301 gttttagagc gtcgtcaacg tgatgcggaa aacaaaagtc agggcaatgt tctagagcgc
      361 cgccaacgtg atgcggataa caagagccaa gtaggtcaac ttatagggaa aaatccactt
      421 ttttcaaagc caactgtatc tagagaaaat aatcactcta gtcaaggtga ctctaacaaa
      481 cagtcattct ctaaaaaagt atctcaggtt actaatgtag ctaatagacc gatgttaact
      541 aataattcta gaacaatttc agtgataaat aaattaccta aaacaggtgg tgatcaaaat
      601 gtcattttta aacttgtagg ttttggttta attttgttaa caagtcgctg cggtttgaga
      661 cgcaatgaaa attaagtata atcaatcatt tagtaactat tataatgata tatacaatca
      721 ataaaaagga atcggatacg agattccttt ttataattag gttggttagg gtgacttttt
      781 tcatttggct attcttgaaa gtttataaaa atgtagtata atagtcacat taaaatgttt
      841 tgaaaatatt gatgaataac atcaacaaat agaggtcatt atatgggata taccgttgct
      901 atcgtgggtg ctacaggtgc cgtaggaaca caaatgattc gtcaattaga acaatcgaat
      961 ttaccaatag aacaagtgaa acttttatca tcaagtcgct cagcaggtaa aattttacat
     1021 tttaaagatg aggctatacg tgttgaagag acaacaaaag aatcatttta cgatgttgat
     1081 attgccttgt tttcagctgg tggatctatt tcagcaaagt tcgctcctta tgcagtaaag
     1141 tctggagcag ttgtagtaga taacacgtca tattttcgtc agaatcctga tgttccacta
     1201 gttgttcctg aagtaaatgc tcatgccatg attggtcata atggtatcat agcttgtccc
     1261 aattgttcta ctattcaaat gatgattgct ttagagccca ttcgtcaaaa atgggggata
     1321 gagcgtgtta tagtttccac ctatcaagct gtttcgggtt caggtgcacg tgctgttgaa
     1381 gaaactaagg aacagttgag acaagtttta aatgataacc tttcacctga tcaattaatt
     1441 gcaacggtct tgccatgttc tagcgatcaa aaacattatc ctatcgcatt caatgcatta
     1501 cctcaaattg atatttttac tgataatgat tatacgtacg aagaaatgaa aatgactttg
     1561 gagacaaaga aaattatgga agatgctact attaaggttt cagctacctg cgtccgcatt
     1621 cccgttttat cgggtcattc tgagtctatt tatattgaga cgaaagaatt agcctcaatc
     1681 tctgaaatta aaaaggctat tgcaaatttt cctggtgccg tcctacaaga ccttccatct
     1741 cagcaaattt accctcaagc gattaatgct gtaggccata gggagacctt tgttggtcgt
     1801 attcgaaaag accttgatca agaaaatggt gttcatatgt gggtagtttc agataatcta
     1861 ctgaaggggg cagcttggaa ttctgttcaa atcgcagaaa cattgcataa gaatgggctg
     1921 gttaaaccag cgaaagagtt aaaatttgaa ttaatataga agaatatgac attgcttaat
     1981 acttatactg acttctagtg atattaattt ttcatcaata tcaggtaaaa cacttgacaa
     2041 attatgaaaa catttgcata atgaaattaa caaatgaaaa tgattgcaaa tcgttttaac
     2101 attgtttcca cattaaggag gggctcttat gcaaatcatc aaacgtgatg gacagattgt
     2161 tgaatttgat cctgaaaaaa tctaccaagc tatcttgaaa gctgctcaaa cagtttatgt
     2221 gcttgatgag tcacttcgtc aagaccttgc tcaagtgact aaaaaagtaa tcttagattt
     2281 agaggatgct caggttgaac gaccaacgat caatatgatt cagtctttgg ttgaaaatcg
     2341 tttattggat gcaggataca ttcatattgc tgaacactat atttcatacc gtttgcaacg
     2401 tgatttagag cgaaatggtt atgatgatca aatcactgtt catttacact ttgaaaaaat
     2461 taagtaaggt ttgactttta agtttaaact ctctatactt gtatatggaa cttactatgt
     2521 aatgtgctaa tttaaaaact tgtagagtgt ctctatgagt tttttaataa cctctattat
     2581 caatcaaagg attttaaatg agtattttag aagtaaaaaa cttaagtcac ggttttggtg
     2641 atcgtgctat ttttgagaat gtttcttttc gccttttaaa aggtgaacat ataggactta
     2701 ttggtgctaa tggtgaagga aaatcaacat tcatgtcaat tgttacaggt catcttcaac
     2761 ctgatgaagg aaaaattgaa tggtcaaaat atgtaactgc tggttacctt gatcaacata
     2821 ctgtgttaga agaaggtcaa acagttcgtg atgttttgcg aacagcattt gatgaacttt
     2881 ttaaaacaga ggcacgtatc aatgatatat atatatccat ggcagatgat ggcgcagatg
     2941 tagatagcct tatggaagaa gttggagaac tacaagatcg tttagaatca cgtgattttt
     3001 atacgcttga tgctaaaatt gatgaagtcg cacgggcatt aggagttatg gattttggta
     3061 tggattctga tgttactgaa ttatctggtg gtcaaaggac aaaggtcctt ttagcaaaac
     3121 tgttattaga aaagccagat atcctcctgc ttgatgagcc tacaaactac cttgatgctg
     3181 aacacattga ttggttaaaa cgctatttac aaaattatga aaatgctttt gttttaatct
     3241 cacacgatat tccatttttg aacgatgtta tcaatattgt ctatcatgtt gaaaatcaag
     3301 atttagtaag atattccggt gattatacta attttgaatc agtctatgca atgaaaaagg
     3361 ctcaattaga agcagcctat gaacgtcaac aaaaggaaat agctgattta caagattttg
     3421 ttaatcgtaa taaagcacgc gttgcaacac gtaatatggc aatgtctcgt caaaagaaac
     3481 tagacaaaat ggatattatt gagttacaag cagagaaacc aaagccaagt tttgaattta
     3541 aagaatctcg cacaccagga cgttttattt tccaagctaa agatcttcaa attggatatg
     3601 atcgtgcttt aacaaaacct ctaaacctaa catttgaacg taatcaaaaa atagctattg
     3661 ttggagcaaa tgggatagga aaaacaacat tacttaagag tttactaggt attatccccc
     3721 ctatttctgg taatgttgaa cgaggtgatt ttattgattt aggttatttt gaacaagaag
     3781 tacccggagg gaatcgccaa actccacttg aagcagtatg ggatgctttt cctgctttga
     3841 accaagcaga ggttagggca gctcttgctc gttgtggttt aacttcaaag catattgaaa
     3901 gtcagattca ggtgttatct ggtggggagc aatcaaaggt cagattttgc ttattaatga
     3961 atcgcgaaaa taatgtcctt gtacttgatg aaccaaccaa tcatttagat gtagatgcta
     4021 aagatgagtt aaaacgtgct ttaaaagctt ataagggatc aatcttgatg gtatgtcatg
     4081 aaccggactt ttatgaagga tggatggatg atgtttggga ctttaaccaa cttagttaag
     4141 tggctagggt aataaagtag aaggcagagc caaataagat gcaaaatgat atgagcgttg
     4201 ttcttagagc tggtaaatta ctgattgaaa gcggagcaga agtctatcgt gtcgaagaca
     4261 caatgaagca ttttgctaag gctcttcaga tagaaaattt tgaagcttat gtggttagta
     4321 gcagtattat cgcttccggg attaaccgct atggtaaaca agaagctaaa gtatgcaata
     4381 cagatggtgt aacagcaaat ttaggtagac ttgaagctgt taataacctt tctaggcaaa
     4441 ttgctaaaca agacttagta tctccagaag aaattgtaaa gcagttagac ttaattgaac
     4501 atcaaaaaga ttatagtttg cttgttactt taatttctta tttttgtgga gcgggtagtt
     4561 tttctttagc cttgggtagt tccttattag attcattttc cgctgctgta acaggcttaa
     4621 ttttaggata ttttttgaat ttgatggaaa gtcgtataca tacaggattt cttttgacca
     4681 ttctaggtag ttctgtagtg gctttaagtg ctaatttatt atatttttca ggattaggtg
     4741 agcatcgtag cattatcata ttaggggcat taatggttat ggtgccaggg gctgcctttg
     4801 ttaattcggt gagagaattt tcacagaata atttttcaac aggtttagct ttgattatgt
     4861 ctgctttact catttgtatt tctatatcag caggcgtggc tattacaatt gaaattattc
     4921 cgtttgcaga acaaatgaca ggttcttttt ctggtgttcc taataccatt cttgagatta
     4981 tcatcagaac actaatggcc ggtttaggga cgatagcctt ctctattctc taccatgttc
     5041 caaaacgtta tttccttgac ttagggatac taggagcaat atcttggatg ctctacctga
     5101 tcttatggca acaatttcac atggatgcta tagccgtatt ttttccaggg cttttcatca
     5161 cttatttttc acggctatta gctgctaaac gtaagtgtcc tgccactgtc tttttagcga
     5221 caagtatgtt tcctcttatc ccaggactta gcttctatag agccgtttac tttttattga
     5281 cgggagcaga tgccttagca atggaatatt ttagatcctg ttttgtaaca gcttttacaa
     5341 ttgctatagc aatcagtatt gtacagcaga ttcctctatc gttctttatt agacgtaaaa
     5401 tgataaaata atttagtatt tatttgacaa aatgattgta aacttgtaga atacctacaa
     5461 atgtatcttt aagtgagtcc agagaggcga acaaggtcac catcataagt caaatagcga
     5521 gtgctttttc gctaactttt tatgaaacct tgccctcagg caaggttttt tgtgtcatac
     5581 tggtacattt ccaacaatta ttatttctat agagaggaat tatatgttag aaaaatgccc
     5641 tatcattgcc ttagattttt ctgatttggc ttctgtaaca acttttttag aacattttcc
     5701 aaaagaagaa ttgctttttg tcaaaatagg aatggagctt tattattccg aaggtccctc
     5761 aattattaga tatattaaat ccttaggaca tcgcattttt ttggatttaa aattacatga
     5821 tatccctaat acagttcgat tatctatgtc tgtcttagcc aaattaggca ttgatatgac
     5881 taatgtccat gctgcaggag gagtcgaaat gatgaaagca gctagagaag gtttggggga
     5941 gggtccaata ctattagcag tgacacaatt aacctcgact tcacaagaac aaatgcaggt
     6001 ggatcaacat attaacttaa gtgtagttga ttcggtttgc cattatgctc aaaaagctca
     6061 agaagcaggc ttagatggag tagtcgcatc ggctcaagag gtaaagcaaa tcaagaaaca
     6121 gactaacgaa catttcattt gcttaacgcc tggtatcaga cctccacaga ccaatcagtt
     6181 agatgatcaa aaaagaacga tgactcctga gcaagcacgt atagttgggg ctgattatat
     6241 cgttgtggga agacctataa cgaaagcaga aaatccatat caagcatatc tagacataaa
     6301 agaagaatgg aatcgtatta aataaaggag tactgagatg gatttagcaa gacagattgc
     6361 catggaacta ttagatattc aagctgttta cttaagaccc caacaaccct ttacttgggc
     6421 ttcgggggtc aaatcgccaa tttacactga taatcgtgta accttatcgt atcctgagac
     6481 aaggacactg attgaaaatg gttttgttaa acaaatacag aaacatttcc caaacgttga
     6541 tatcatcgca ggaacagcga ctgcaggcat accacatggc gctattatag ctgataagat
     6601 gaacttgccc tttgcctata tacgctcgaa ggcaaaggat catggtgtgg ggaatcaaat
     6661 cgagggacgt gtttactcag gccaaaaaat ggttatcatt gaagatttga tttcaacagg
     6721 aggatctgtt ttagaggctg ttacggctgc gcagagtcaa ggtattgagg ttttaggggt
     6781 ggtagcgata tttacatatc aattagctaa ggctgaacaa gcatttcgtg aagctgatat
     6841 tcctttggtg actttgacgg attacaatca attaattaaa gttgctaaag ttaatggata
     6901 tattacagct gaccaactgg tattactgaa aaaatttaaa gaagatcaaa tgaattggca
     6961 aagttagaga ggacagatat gtatatcatt aaaaacggat tgattatcga ccctcaaagt
     7021 ggttttaatc aagtatcaga catgctgatt gatcaaggaa aaattaaaca aatatcgaaa
     7081 gaaattgata ttaaaggcat tccaatcatt gatgcaagca ataaaattgt agctccagga
     7141 ttagtcgata ttcatgtaca ttttcgtgag cctggacaga cacataaaga aaatatccat
     7201 acaggagcct tatcagctgc agcaggtggt tttacgacag ttcttatgat ggccaatact
     7261 aacccaacga tttctagctc agaaattgta aaacaagtta aggagagtgc agcaaaagaa
     7321 gcaattaaga ttgaaacagt tgctacaatt actaaatccc tgaatggtaa agatttagtt
     7381 aatttcgaag aattattaga agctggagtt gctggattct cagacgatgg tatccctttg
     7441 acagatacta aggttttgca agaagctatg aacttagcta gaaaacatga tgttgtccta
     7501 tcgttacatg aagaggatcc atcgttaaat ggtgttcttg gtatcaacga acatattgct
     7561 cagaaaattt atcatgtctg tggagctagt ggtcttgctg aatattcaat gattgcgcga
     7621 gatgctatga ttgcttacca aacacaggcg aaagtccata tccagcattt gtcaagttca
     7681 gaatcagtag aagtagttga ttttgcgcag aagcttggtg ctaacttaac agcagaagta
     7741 accccccaac atttttcaaa gacagaggac ttacttttaa ctaagggtgc taatgcaaaa
     7801 ttaaatccac cactgcgact tgaaaaggat agacaagccc tcattgatgg tcttaaaagt
     7861 ggtgttattt caattattgc aagtgatcac gctccccatc atattatgga gaaagcagca
     7921 gataatatta gtcaagcgcc ctctggtatg actggcttag agacatcatt agccttaggg
     7981 attacttatt tagtatctac taaagaactc tcaatgattg actttttggc aaaaatgaca
     8041 tgtaatcctg cacagttata tggatttgat gcaggttatc tcagagaagg tggacctgca
     8101 gatatcgtta tttttgatca agctgaagag agaataataa aggcagaatt tgcctccaaa
     8161 tccagcaact ctccttttat cggtgataaa ttgaagggag ttgttcacta cacaatttgc
     8221 aatggtgaaa tagtctatca aaaagatagc cacgcagcga cattggtata ggtaaaacat
     8281 ttcttattaa tatcctttaa ggtattgtcc agagaggcaa acaaggaatt gcgttaacaa
     8341 atagtaagtt agttccctat ctagcttact attttgcaga gcctccttgt tttaggaggc
     8401 ttttttctag ctaaaattta tagaaagaaa ttatcatgaa tcatcaaaca caaacacttt
     8461 cattagaaca ttttgtcagt ttagaagaat tatcaaatca agaagttatg tctttgataa
     8521 agagaagtat cgaagttaaa gaaaatccta gcaacattgg ttttgataaa gattactatg
     8581 tgtcaaatct cttttttgag aattcaacaa gaacacataa gtcatttgaa atggcagaac
     8641 tcaaattagg attaaaaaca attgaattta atgctgatac aagttctgtt aataaaggag
     8701 aaacccttta tgacactatc ttaacaatga gtgctctagg attggacgtt tgtgtcatta
     8761 ggcaccctga tattgactat tataaagaat tgattgctag tccgaacatt cattcagcta
     8821 ttgtcaatgg gggtgatggc tcaggccaac atccaagcca atcacttttg gatttggtaa
     8881 ctatttacga agaatttgga tacttcaaag gactaaaaat tgcgattgta ggagatctaa
     8941 ctcactcacg ggtagctaaa tctaacatgc aagtgctaaa acgattagga gctgaaattt
     9001 tcttttctgg acccaaagaa tggtatagta gtcagttcga tgaatatggg caatgtttac
     9061 caatagatca gttagttgat caaattgatg tccttatgct tttacgtgtt caacacgaac
     9121 gtcatgatgg taaaggggtg ttttcaaaag aaagctatca tcaacaattt ggtcttacaa
     9181 aagaacgcta taaacattta agggatacag caattattat gcacccagca cccgtcaata
     9241 gggatgtaga gattgctagc gatttagtag aagcagataa ggctcgaata gtgaaacaga
     9301 tgagtaatgg cgtctatgca aggatagcta tcttagaagc agtgctcaat agcagataat
     9361 aaaggagata aaatgaagcg tctattatta ttggaagatg gtagtgtgtt tgagggtgag
     9421 gctttcggag cggatgtagg aacaagtggt gaaattgttt ttagtacagg aatgacaggg
     9481 tatcaagaat ctataacaga tcaatcttac aacggtcaaa tcattacttt tacttatcct
     9541 cttattggaa attatgggat aaatcgagac gattacgaat ctattagacc aacatgtaag
     9601 ggagtaattg tttatgaatg ggcagagtat ccaagtaact ggcgtcaaca gatgacattt
     9661 gatgaatttt taaaattaaa aggaatacct ggaatttcag gtatagatac tcgagcatta
     9721 acaaaaatta ttcgaaaaca tggaacaatg aaagcttgtt taataaacga gggaaattct
     9781 attcatgaag cactagaaaa ccttcaaaaa agtgttcttt taaataatca aattgaacaa
     9841 gtatcaacca aattagctta tgcttcgcct ggagttggca aaaatattgt attagttgac
     9901 tttggtttaa aacattctat tctaagagaa ctgtcacaac gtcaatgtca tattacagtc
     9961 gtccctcata ctacgacagc gcaagaaatc ttaaatctca atccagatgg agtactctta
    10021 tctaacggcc ctgggaaccc agaacaatta ccgaatgctt tacaaatgat ccaagaaatt
    10081 caaggtaaaa ttccaatttt tggtatttgt atgggacatc aactatttgc taaagccaat
    10141 ggtgcaaaga cttataaaat gacttttggt catcgaggtt ttaaccatgc tgttcgtcat
    10201 ttgcaaacag gacaggttga ttttacaagt caaaatcatg gttatgctgt ctcgagagaa
    10261 gatttccctg aggctctctt cattacacat gaagagatta atgataaaac cgtcgaaggt
    10321 gttcgacata aatactatcc tgccttttca gtgcagtttc acccagatgc agcaccaggg
    10381 ccgcatgata catcttatct ttttgatgaa tttattaata tgattgatga tttccagcaa
    10441 aaaagctagc tttaaaatat agaattagag gagtttgata tgccaaagcg cacagacata
    10501 cgtaaaatta tggttatcgg ttctggcccg attgtgattg gacaagcagc agagtttgac
    10561 tattctggta cacaagcctg tctctcttta aaagaggagg gataccaagt tgttttggtc
    10621 aattcgaatc cggctactat catgacagat aaagatattg ctgataaagt ttatattgaa
    10681 cctattacct tggagtttgt cacaagaatt ctcaggaagg aaagacctga tgctctcctt
    10741 ccaactcttg gaggtcaaac tggattgaat atggctatgg ctctatcaaa aaatggtatc
    10801 ctagaagaat tgaatgttga actattaggg accaaattat cagccattga taaagctgaa
    10861 gatcgtgatt tatttaaaca gttgatggaa gaactcaatc aacctattcc agaatccgag
    10921 attgttaatt cggtagaaga agctattcaa tttgcagagc aaatcgggta tccattaatt
    10981 gttcgtcctg cctttacttt aggaggtaca ggtggaggca tgtgcgataa tcaagaacaa
    11041 ttggttgaca tcacgacgaa ggggttaaag ttatcgcctg tgacacagtg ccttattgaa
    11101 cgttcaattg ctggctttaa agagattgaa tacgaagtta tgcgtgatgc agcagataac
    11161 gctcttgttg tttgtaatat ggagaatttt gatcctgttg gtatccatac aggggactct
    11221 atagtttttg cacccgcaca aacgttatct gatgtggaaa atcagttatt acgagatgcg
    11281 agtttagata ttataagagc tttgaaaatt gaaggaggat gcaatgttca actagccctt
    11341 gatccgaata gttttaaata ctatgtcatt gaagttaatc caagagtatc aagatcttct
    11401 gctttagctt caaaagctac tggatatcca attgctaaat tagcagctaa gatagctgtt
    11461 ggtttgacac tagatgaggt aattaatcct attacaaaaa cgacttacgc tatgtttgaa
    11521 cctgctttag attacgtagt cgcaaaaatg ccgagatttc cttttgataa atttgaaagt
    11581 ggcgatagaa aattgggaac acagatgaaa gctacaggcg aagtcatggc aataggacgg
    11641 aatatagaag aatctttgtt gaaagcttgt cgttcactag aaataggtgt cgatcacatt
    11701 aaaatagcag atttagataa tgtatcagat gatgttttat tagaaaaaat aagaaaggca
    11761 gaagatgatc gcttatttta tctagcagaa gctttacgta gacattatag tattgaaaaa
    11821 ttagcaagtt tgactagcat tgattctttc ttccttgata agttaagagt gattgtagag
    11881 ttagaagatc tattatctaa aaacagactg gatatcaata ttctcaaaaa ggttaaaaat
    11941 aaaggttttt cagataaagc tatagcgagt ttatggcaaa taaacgaaga tcaagttcgt
    12001 aacatgcgaa aagaagcagg aattcttcca gtttataaga tggttgatac atgtgcggct
    12061 gagtttgatt cagcaacgcc ctacttttat tcgacttatg ccgtagaaaa cgagtcattg
    12121 atatcagata aagcctctat tttggtttta ggatcgggcc caatccgaat tggacaggga
    12181 gttgaatttg attattcaac ggttcattct gttaaagcca tcagagagtc tggttttgaa
    12241 gcgattatca tgaactctaa tccagagacg gtctcaacgg atttctctat ttcagataag
    12301 ctttactttg aacctttgac ttttgaagat gttatgaatg ttattgactt agaaaaacct
    12361 gaaggggtca tcttacaatt cggtggtcag actgctatta atctagcaaa agatttaaac
    12421 aaggcagggg ttaaaatact aggcacacaa ttagaagatt tggatcgtgc tgaaaataga
    12481 aaacaatttg aagccacttt acaagctcta aatatccctc aaccaccagg ttttaccgcc
    12541 actacggagg aagaggcagt taatgcagcg caaaagattg gttatcccgt gttggttaga
    12601 ccatcatatg ttcttggtgg acgagctatg aagatagttg aaaacgaaga agatttacgt
    12661 cattatatga caaccgcggt aaaggcaagc ccagatcatc cagtgcttat agatgcatac
    12721 cttataggta aagagtgtga ggttgatgct atatccgatg gtcagaatat actgattcct
    12781 ggaataatgg aacatataga gcgagctggt gttcattcag gagattccat ggctgtatac
    12841 cctcctcaaa cattgtctga gaccattatc gaaactattg tagattatac aaaacgctta
    12901 gcaattggat taaactgtat cggtatgatg aatattcagt ttgtaatcaa ggatcagaaa
    12961 gtatatgtta ttgaagttaa tcctcgtgct ggtagaacac ttccattctt atcaaaagta
    13021 actcacattc ctatggccca agtagcaaca aaagttatct taggagataa actctgcaat
    13081 tttacatatg gttatgatct ctatccagct tcagatatgg tccacattaa agcccctgtc
    13141 tttagtttta ccaaacttgc taaagtagat agtttattag gtcctgaaat gaaatcaaca
    13201 ggcgaagtga tgggatcaga tattaatctt cagaaagcct tgtataaagc ctttgaagca
    13261 gcatatctcc atatgccaga ctatggtaac attgttttta cagtagatga tacagacaaa
    13321 gaagaagctt tagaacttgc aaaagtctat caaagtattg gttatcgtat ttatgctacc
    13381 caagggactg caatttattt tgatgctaat ggtttagaga cagttttagt aggtaagtta
    13441 ggagagaatg atcgaaatca tattcctgat ttaattaaaa atggtaaaat tcaagcagtt
    13501 atcaacacag ttgggcaaaa taacattgat aaccatgatg ctctcatcat cagacgttct
    13561 gcaattgaac aaggagttcc tctatttaca tctttagata ctgcacatgc aatgttcaaa
    13621 gttcttgaaa gcaaagcatt tacattaaaa gtactagatt aatcaaacaa tcaaggtaaa
    13681 attaacttcc agcaaaacag aatcggacta gattaaatat agtttgttct gtttttataa
    13741 aaaagcctaa ttgtgataaa atagggaaca aacttaagga gttaattatg ttaaaaacac
    13801 gaacacatat taatgaatta gaaacaaaaa tcgcccttga tttagtacac agatatctag
    13861 gtattcaaca acttgttttt tcaaagtgga gacattcttt agaaactggt tcatggtctt
    13921 ctattataac aataaatggc gctaatatta tcaattacat tgttgatgat attagcgaca
    13981 gtgatagtat ttcaggtatt atttgtcatc ctaaaagttt tcatctcaga cctagagaaa
    14041 aaaaggaacc tattcagtct attgatgagt tagatatcat ctggtctttg aatagagagg
    14101 aaaatgaatg attccagata gtagttttaa agtgctatca gatatggcat ataatattga
    14161 aaaatccaaa gcagaaagta atggacacct taaaattata gaaggtagtc aaattattga
    14221 tttccatcaa aaacgctctt ttatggtatt agaggttaat gataatatcg ataatggtat
    14281 gcaggctatg gcatgtcagt ctttggatga gagtaatcat attgttatag catattcagg
    14341 aacgaacgct tttaataaga aagatatctt aacagatatc gattcagttg tattggggaa
    14401 gaacaataaa ttgctaactt gtccttcaag tattaccctg agagaggatg gcatttctct
    14461 gccttgtaaa aatccaagtc aaattacaac atctaaacta tttgcacaaa aaattaagat
    14521 gaactatcct gatgcaaccc tctggttaac aggccatggt aaatgttcag tcaggtaata
    14581 tctcaaatta ccaaaaaaat cttttagatt ctgcaactaa tttccaaaat atctttccag
    14641 ccctcgttaa ccagtatagt agttccatta ctgctaacga atcactgaaa aaatctttag
    14701 aagcttctga taacatgttt aatgatttga tgacaaccca gacaaatact ggaaaagatt
    14761 tatcaagctt aatagaacag cgccatcaag atagcatttc gtatgaagcg ttttcggcct
    14821 cattactaga aatgaataac gagttattag agaagcaatt atctgatatt atcacacaag
    14881 cacaaaaaga ccaagaaaca ttatcatcac aactcaatag tattatgggt gatgataacg
    14941 ataatgatca taatcataaa gaaaactcat cagcttatct aaatgttgca aggcaaaaaa
    15001 tccaagaact atctgaagca ctcaagtcac aagataacat cgctaaagat caaagtgaac
    15061 aactagataa aattgttaga gagggactag caagttactt tgctaagaat aataaagata
    15121 atattacttt attagaatta ttgaagagtc attctactaa tgagaagact ttgaaagatt
    15181 ttaaagttaa ggtagcagat ttcacaaatt ctctcatttc aagcattccc tcactaaacc
    15241 tttcggagtt gcacttaacc caagaagaaa aagccattca gtttacatct tctgattcgg
    15301 aaatcattaa aaaagtaagc aatgaaagaa ctctgtattt aaatactaat ttattgaatc
    15361 gtctttttga agctaggaaa aatagagatg aagccaaaaa taaagttaat caattaaaat
    15421 tgccatctag ttctactcga actggtgagc agatagtttc tgttgaatca aataatccag
    15481 attacagggt agatacctgg acggttaatg gtaagcaaac aagaaccttg gaccccagcc
    15541 aaaacaataa tattattatc aatagcagtt atcaagggga attatctaac tctagtatta
    15601 caaaaccaag ctatacaatc actatcggga atcagaaaca aattgtacaa aatgatgacg
    15661 gtgaaattca acggtcttac ttggaggcag aagcaacgta tcaacgaact ctacaagaag
    15721 taattgatgc atataacacc actcacgact tagtagctaa atactacatt atttctgatg
    15781 gcgaagaacc gcaaaattta tttgatcaat ttttaaatca aagtgttaac gatacgatgg
    15841 ttgatctggt caaaaatggt ataactaagt atttgatgga tgaaaacact gctgatgcgc
    15901 aacagaaagt taaagatgtc atggaggaag ttgaaaatag ccaagatgaa ttggcggatc
    15961 aaatggcaaa agtaactgag acaaatgtac gactaacaga tacaatcaaa aaacagcttg
    16021 agactcttca aacaattaac atgaaggtcc aaaacatcac acaggatcaa tctaaagtaa
    16081 acgactcaca gaaaacaact gatcaacaat tatctgattt gaagaaccag ctagatgggc
    16141 taatgacctc tgcaactggc gtaaaagata tgtccaaatc taatagtcaa gaggcggacc
    16201 aagttaatca aatattcaca tcattcaata aagatgttca agatgctaaa aattcgggca
    16261 acaaactttc gacggatgca acagatttaa tggctaactt ccaaaaagaa ttggccaata
    16321 acggtgattt cgtagcttct ttctctaaag tttttaatac tgcttataaa aatggagtgc
    16381 caaatgatat tctccttaat ttcttatcac gacctgtcgc tgaatcagcg tctgcagtta
    16441 gggcaactga aaatacttat cgcccattca cttggatact attattagaa gttgttagcc
    16501 tatttatagc atatatcttt gcgactcaaa accttattaa gaaattgaca gataggtata
    16561 atgtcaatcg ctggcttcaa acagactttt taaatatcat tgtcatttca ggcctctctc
    16621 ttgtgattgg tttagcactg ggagttatct caagcagaag tcttcatgtc atgcctgaat
    16681 atgtaccatc ttgg
//
LOCUS       DATRNX010000041        15934 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRNX010000041 DATRNX010000000
VERSION     DATRNX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..743)
                     /locus_tag="VBC81_001052"
     CDS             complement(<1..743)
                     /locus_tag="VBC81_001052"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6912101.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSN"
     gene            849..1439
                     /locus_tag="VBC81_001053"
     CDS             849..1439
                     /locus_tag="VBC81_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6912102.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            1765..2154
                     /locus_tag="VBC81_001054"
     CDS             1765..2154
                     /locus_tag="VBC81_001054"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6912103.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            2219..3268
                     /locus_tag="VBC81_001055"
     CDS             2219..3268
                     /locus_tag="VBC81_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6912104.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            3413..4948
                     /locus_tag="VBC81_001056"
     CDS             3413..4948
                     /locus_tag="VBC81_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912105.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            4941..6002
                     /locus_tag="VBC81_001057"
     CDS             4941..6002
                     /locus_tag="VBC81_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912106.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFQLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            6004..6960
                     /locus_tag="VBC81_001058"
     CDS             6004..6960
                     /locus_tag="VBC81_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912107.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            7166..8536
                     /locus_tag="VBC81_001059"
     CDS             7166..8536
                     /locus_tag="VBC81_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6912108.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(8657..9646)
                     /locus_tag="VBC81_001060"
     CDS             complement(8657..9646)
                     /locus_tag="VBC81_001060"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6912109.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            9885..12344
                     /gene="gyrA"
                     /locus_tag="VBC81_001061"
     CDS             9885..12344
                     /gene="gyrA"
                     /locus_tag="VBC81_001061"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6912110.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            12351..13094
                     /locus_tag="VBC81_001062"
     CDS             12351..13094
                     /locus_tag="VBC81_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6912111.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            13110..13523
                     /locus_tag="VBC81_001063"
     CDS             13110..13523
                     /locus_tag="VBC81_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6912112.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            13676..14638
                     /locus_tag="VBC81_001064"
     CDS             13676..14638
                     /locus_tag="VBC81_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6912113.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
     gene            complement(14701..15828)
                     /locus_tag="VBC81_001065"
     CDS             complement(14701..15828)
                     /locus_tag="VBC81_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6912114.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
BASE COUNT         5283 a         2424 c         3189 g         5038 t
ORIGIN      
        1 taattactag ggtctgcttc ggctaattgt agaagactgt taaatcgttg taaataccat
       61 tcttcaattt gtttattttc agcatcaatg tatattgaaa aatcaaaata atcattcatg
      121 taaagtcgat ggttttgttg gttttgaaag acatttattc cttctaaaat taaaaaatct
      181 ggtgtttcaa tagtttgcaa ttgatttgga acgatatcgt agatttcatg agaataaatt
      241 gggattttcg cggttatacc gttctttata gtatctaaga aatttagaag tgactccata
      301 tcataggatt ccgggaaacc tttacggttt aaaattccat tttgtatgag tttctcgtta
      361 ggatataaaa agccatcagt agtgactaat tcgacgtgac tatctttaaa agttcgtgaa
      421 attagtaatt gtagtaagcg actggtagtt gattttccaa cagcaactga ccctgatatg
      481 ccgataataa aaggacgttg ataattttcc tttttaagaa atattgcttt agaaaaagat
      541 aaattttctt gactacgttt atagatttga ataaggttaa ttaaaggaag gtagatgtca
      601 ataacatctt gaatattaat attatcgttt aagcttttga tagattcaag ctctttttca
      661 gttagtaaag cttgggattg ctgatgtaag tccttccaat tttctctgga aatacggtcg
      721 aaattaataa attcattatt catgtcaaac tcctatatga aagctattat aacattttat
      781 tgagtgattg aaaaccgttt caaaatatgg taaaataata tggcgaaatt ggagggttag
      841 aattatttat ggctaatatg tattatacag agaatcctaa tgttgaacat gatattcatg
      901 agctaaatgt caaattatta ggagaatcat tcagtttttt aactgatgca ggagttttct
      961 ctaaaagaat gatagattat ggtagtcaag tccttctaaa tagtttacat tttgaaaaaa
     1021 ataaatcctt gttagacctt gggtgtggat acggtccgct gggaatttct ctagccaaag
     1081 tgcaaggagt taaagcaact atggttgaca ttaatacgag agcattggaa ttagctaaga
     1141 aaaatgcaac acgcaatgga gtagtagttg aagtctttca gtcaaacatt tatgaaaata
     1201 tttctaaaac tttcgactat attataagca acccacctat tcgtgctgga aagcaagttg
     1261 ttcattctat tatcgaagaa agtatctgtt accttaatac aggcggtagc cttaccattg
     1321 tcattcaaaa gaaacaaggc gctccaagtg caaaagctaa aatgctagat acttttggta
     1381 attgcgatat tttaaaaaaa gataagggat attatatatt gagaagtgaa aaagtctaaa
     1441 tgatatccct aaagcgtaaa aataataaat agttcggata ataactatcg tattattttt
     1501 tggaaaggtt ttctctaaaa atgttttctt taaattccgt ataagataaa aagaattctg
     1561 ctttttattc tgaaaattat aaaaaactac ttacattatc taaaaaaaat ggtaaaatag
     1621 attcagctaa aataccaaga ctaatgaaac tacaagactt ggttactcaa taataaatac
     1681 ttctgtgttt gataagaaga taataaacgt gttctaaaaa ttttgccagt aaacattatg
     1741 aagcgaaagg tgcagctact aaaaatggga aatatagagt tgaaaaaatt ggctgtaaaa
     1801 gcgagtgaaa atgcatacgt tccttactcg aaatttccag taggcgcagc attgcgtaca
     1861 gcagaaggaa aaatatttac aggatgtaac gttgaaaata tcagttatgg attagcaaac
     1921 tgtgctgagc gtactgcaat ttttaaagct gtttcagaag gttataaaga tttttcggaa
     1981 attgctattt atggaaatac tgaagaacca atttctcctt gcggtgcatg ccgtcaagtg
     2041 atggtggaat tttttaataa gaatgctaaa gttacattga ttgctaaaaa tggtaagacg
     2101 gtcgagacga cggtcggtga gttgttacca tactcgtttg ttgatttaaa ataatttaaa
     2161 tcagttgctc ttttaagcat ttagcttaaa tattaagttt tttaggaggg ttcatttcat
     2221 gaacaaaaaa attgccggga tcggcttggc ttcgattgca gtacttagtt tagctgcatg
     2281 tggacatcgt ggtgcttcta aatctggtgg taaatcagat agcttgaagg ttgcaatggt
     2341 aacagatacc ggtggtgttg atgataaatc atttaaccaa tctggttggg aaggtatgca
     2401 agcttggggc aagaagaatg gccttaaaaa aggagctggt tttgactatt tccaatcggc
     2461 aagtgaatct gattatgcaa ctaacttaga tacagctgtg tctagtggtt ataaattgat
     2521 tttcggtatt ggattttctc ttcatgatgc tattgataaa gcagcagaca ataacaaaga
     2581 tgttaattac gtcatcgttg atgatgttat taaagggaaa gataatgttg caagtgttgt
     2641 ctttgcggat aatgaatcag cttacttagc aggtattgca gccgctaaaa ctaccaaaac
     2701 aaaaacagtt ggctttgtag gtggtatgga atctgaggtt attacccgtt ttgaaaaagg
     2761 ttttgaagca ggtgtcaaat cagttgataa atcaattaaa attaaagttg actatgctgg
     2821 ttcattcggt gatgctgcta agggtaagac aattgcagcc gcacaatatg cttctggcgc
     2881 agatattgtt tatcaagtag ctggtggaac tggtgcaggt gtattcagtg aagcaaaatc
     2941 acgcaatgaa tctttaaaag aagctgacaa agtttgggta ttaggtgtag accgtgacca
     3001 agcagcagaa ggtaagtata cttctaaaga tggtaaggct tctaactttg ttttggcttc
     3061 atcaattaaa gaagttggaa aatcagttga attgattgct actaaaacaa gtaaaggtaa
     3121 attcccaggt ggtaatgtaa caacttatgg tttgaaggat ggtggagttg atatagcaac
     3181 tacaaacctt tcagatgacg ccgttaaagc gattaaagag gctaaagcta aaattatttc
     3241 aggagatatc aaagttccta gcaaataata tctttaagaa ctttcaaaaa agcgattaga
     3301 aatcgctttt ttgaagctga gataaactta tatctcagta aaatccatca gtcgttaacg
     3361 attggtggat tttactgaac tataaatgaa tttttctgaa agggagaacc acatgacaca
     3421 gaatgtcatt gaaatgaaag aaatcactaa gaaattcggt gattttgtgg caaatgatca
     3481 tatcaatttg acggttgaaa aaggtgagat acatgctctg cttggcgaaa acggtgcagg
     3541 gaaatcaact ttaatgaaca tgttagcagg tttactagaa ccaacagacg gtcaaatttt
     3601 tattaatggt cagcctgtaa ctattgattc accttccaaa tcgtcacaat tggggattgg
     3661 tatggtacat cagcatttca tgcttgttga agcatttact gtagctgaaa atattgttct
     3721 aggaaatgaa accacccaaa atggtgtttt agacattaag acagcagcta aagaaataaa
     3781 ggaactatca gagaaatatg gtttgtcagt aaatccaaat gctaaaatat ctgatatttc
     3841 tgtaggagca cagcagcgtg ttgaaatttt gaagacactt tatcgcggtg cagatatttt
     3901 aatttttgat gagccgacgg cggtgcttac accatcagaa ataaaagaat tgatgacaat
     3961 tatgaagagc cttgttaaag aaggcaaatc cattatacta attacccata aattggatga
     4021 aattcgggca gttgctgata aagttactgt gattcgccgt ggtaaaagta ttgaaacagt
     4081 ccctgttgca ggcgcaagtt ctcaacaact ggcggaaatg atggttggac gatctgtttc
     4141 cttcagaact gaaaaaaagg aagctaatcc gactgacata atcctttctg taaaggattt
     4201 agttgtagaa gaaaatagag gcggcgttct agctgttaaa aatctgtcgt tagatgttcg
     4261 agcaggtgaa attgtaggta tagctggtat tgatggtaat ggacaaagtg aattaattca
     4321 agctattaca ggtttaagaa aggtaacttc tggtcaaatt gtaattaaag gtaaagatgt
     4381 cacaaaattt tcaagtcgac aaattacaga attaagtgta ggtcatgttc cagaagatcg
     4441 tcatagagat ggtttggttc tagatatgac gatggcagaa aatttagctt tacaaactta
     4501 ttataaagag ccattgagtc ataaaggtat ccttaatttt gctaaaatta aagagtatgc
     4561 tcgtcaatta atgacagaat ttgatgtgcg tggtgctggt gaacatgttt tagcaagagg
     4621 attttctggt ggtaaccaac aaaaagctat tattgctcgt gaagtggatc gcgatccaga
     4681 cctactcatt gttagccaac cgactcgtgg tctagatgtt ggtgctatcg agtatattca
     4741 taaacgtttg attgaagagc gcgataaagg gaaagcggtc ttagtagtta gctttgagtt
     4801 agatgaaatc ttaaacttat cagatcgtat tgcagttatt catgatggta aaattcaagg
     4861 aattgttaaa cccgaccaaa ccaacaagca agaattgggt attttgatgg ctggtggaaa
     4921 aattgaaaag gaggagagag atgtctaaga aggcgcaaaa aattgcagtt cctctgattt
     4981 cagttgtact aggaatcatt ctaggagcta ttattatgct gatatttggt tatgatccac
     5041 tttggggtta tgagggattg ttccaaacag cattcggtag tataaaaaat attggtgaaa
     5101 ttttccgtgc aatgggacca ttaattttaa ttgctctagg tttctcagtt gccagtcgtg
     5161 caggtttctt taatattgga cttccagggc aagccttatc aggatggatt gccgcaggtt
     5221 ggttcgctct ttcacaccca gatatgccac gtccagcaat gattctttgt actattatta
     5281 ttggtatcgt tgctggggga atcactggag ctattccagg tattctcaga gcataccttg
     5341 gaacaagtga agttattgta actattatga tgaactatat tgttctttat tcaggaaatg
     5401 ctattgtaca acgtgttttc ccaaaaagca ttatgcgtac aagtgactcg agtgtatatg
     5461 tttctgccaa tgcatcatac cagacagatt ggttatctag tttaacgaat aattcacgta
     5521 tcaatattgg tatcttcatt gctattattg cagttgtgtt agtttggttc caattaaata
     5581 aaacaacgct tggatttgaa attcgttcag ttggtttaaa tccaaatgct agtgagtacg
     5641 ctggtatgtc tgcaaagcgt acaattattc tatcaatgat tatttctgga gcttttgcag
     5701 gacttggtgg tgttgttgaa ggactaggta catttgagaa tgtatttgta cagccatcct
     5761 cgttagctat tggctttgac ggtatggcag taagtctact cgcagcgaat agtccaatag
     5821 gtattttatt tgcagcattt ctatttggtg ttctaagtgt tggtgcgcct ggtatgaaca
     5881 ttgcaggtat tccacctgag ttaattaaag ttgtaacagc ttcaatcatc ttcttcgtag
     5941 gtgtccatta cattattgag tatgttatta aacctaaaaa acaaatgaaa ggtggtaagt
     6001 aaattgagtt taacaacgat ttttgcattg ctattttcat caatgctaat ttacgcaaca
     6061 cctcttatct ttacaagtat tgggggaacc ttctctgaac gtggtggtat cgtcaacgtt
     6121 ggtttagaag gaattatggt aattggagct ttctcaggcg ttgtatttaa tttagagttt
     6181 gcttctgttt ttggtgatgc gactccatgg atttctgtac tagttggtgg tttagttggt
     6241 ttaatttttt ctgttatcca tgcagttgcg acagttaatt ttcgtgcaga tcatattatc
     6301 agcggaaccg ttcttaattt aatggcacct tcattagctg ttttcttgat taaggttctt
     6361 tataataaag gtcaaactga taatattcaa gaatcatttg gaaaatttaa tttccctata
     6421 ctatcagata ttccttttgt tggtgatatc ttctttaaag gaacaagctt agtcggttat
     6481 attgctattc ttttctcatt ccttgcttgg tttattctat ataaaactcg ctttggatta
     6541 agattacgct cagttggaga acatcctcaa gcagcagaca ccttaggtat taatgtttat
     6601 cttatgcgtt actctggtgt cttgatttca ggtttccttg gtggaatagg aggagctgta
     6661 tatgctcaat ctatttctgt taactttgca gcgacaacta tattaggacc gggctttatc
     6721 tccttagcgg cgatgatttt tggtaagtgg aatccaattg gtgcaatgct tgctagtcta
     6781 ttctttggtt tatcgcaaag tttagcagtt atcggtagcc atttaccact tttgagcaat
     6841 attccaacag tttatctaca aattgctcca tatgtcctca ctatcattgt attagcagct
     6901 ttctttggac aagcagtagc tcctaaggcg gatggtatta attatattaa aacgaaataa
     6961 ataaaaagaa actagtataa gctagtttct ttttattgtt tgtgaatttg tgaaaaaaat
     7021 actttgtgaa aatttcacat aaattataat acaataacaa taaaatataa aaaaatagaa
     7081 aattgagtca ttattctagt aagagtttgt gaataatgtt ataatgtgtt ttgttattaa
     7141 ttatttataa aggagaactc ttaatatgag taaaatcgtt gtcgtaggga caaaccacgc
     7201 tggtacagca gcaattaaaa caatgcttag taattatggt gaagccaatg aaattgtaac
     7261 atttgatcaa aattccaata tctctttctt aggatgtggt atggctcttt ggattggtga
     7321 acagattgat ggtccagaag gattgttcta ctctgataaa gagcagttgg aaagtatggg
     7381 agctaaggtt tacatgaact ctccagtact taatattgac tacgataaaa aagaagttac
     7441 agctttagta gatggaaaag aacatgttga aagttatgaa aaacttattt tagcaactgg
     7501 ttcacaacct atcattccac caattaaagg tgttgaaatt caagaaggat ctcgtgaatt
     7561 taaagctact cttgaaaacc ttcaatttgt gaaactttat caaaattcag aagaagttat
     7621 tgaaaagtta gctaaacctg gtatcaatcg tgttgctgtt gttggggctg ggtatattgg
     7681 tgtagaatta gcagaagctt tccaacgtat tggaaaagaa gtaacacttg ttgatgttgc
     7741 tgatacttgt atgggtggtt actatgatcg tgactttact gatatgatga gtaaaaacct
     7801 tgaagaccat ggtattcgtc ttgctttcgg acaagctgtt caagcagttg aaggtgatgg
     7861 taaagtagaa cgtcttgtta ctgataaaga aacatttgat gttgatatgg tgatcttagc
     7921 tgttggattc cgtccaaata cagaactcgg tgcaggtaaa ttagatacat tccgtaatgg
     7981 cgcttgggta gttgataaga agcaagaaac aagcgtgaaa gatgtttatg ctataggtga
     8041 ttgtgcgaca atttgggata attctcgtga tgatatcaat tatattgctc ttgcttcaaa
     8101 cgccgttcgt actggtattg tagcagctca caatgcatgt ggtacagagc ttgaaggtgc
     8161 tggtgtccaa gggtcaaatg gtatctcaat ctatggcctt aacatggtgt caactggttt
     8221 gactttagaa aaagcaaaac aagctgggta caatgcagtt gaaacaggct ttaatgacct
     8281 tcaaaaacca gaatttatca aacacaataa tcatgaagta gctattaaga ttgtctatga
     8341 taaagatagc cgagttatcc ttggttgcca aatggtctca cacgaagatg tatctatggg
     8401 aattcatatg ttctcacttg ctattcaaga aaaagttact attgaaaagc ttgctttgac
     8461 cgatatcttc ttcttaccac attttaataa gccatataac tatattacaa tggctgcatt
     8521 aggcgctaaa gattaattta aaattaaaaa ggttaagcga aatgcttagc cttttatttc
     8581 atcctttaac aaggtcaaaa atgtaacggc aaagaaaaag ctaagatatt cctatcctag
     8641 ctttttttca actatgttag ttttttgatg cttcttggaa ttctgggttt ttccaagctt
     8701 cgtcaataat atcttttaat tgttcagctg aggcttgcat tttttgaagt tcagcatcat
     8761 ttaatgggat attaactgga cgaacgatac catgtgcacc tacgattgca ggctgaccga
     8821 taaagacatc tttaacatca ccgtattggc cttcttgata tacagataat ggaagaactg
     8881 cattttcatc atcaaggata gctttagtaa tacgagcaag tgcgacagca ataccgtaat
     8941 atgtagcacc ttttttgttg atgattgagt atgcagcgtc acgaactgaa ataaacaaat
     9001 caacaagtcc ttgttcatca atatcgcgat tttcttgtaa ccattgttca agttggacac
     9061 cagcaacgtt agcatgtgac caaacggcaa attctgagtc accgtgttca cccatgatgt
     9121 atgcgtgaac tgaacgagca tcaacaccaa ttttatctgc taaagcttga cggaaacgtg
     9181 cagagtcaag tgaagtacct gaaccaataa cgcgttcctt agggaaacct gagaatttcc
     9241 aagttgaata tgtaagtaca tctacagggt tagccgctac taagaagata ccgttgaaac
     9301 ctgattcaac aacttgagtt acgattgatt tgttaattgc taagttttta ccaacaaggt
     9361 caagtcgagt ttcacctggt ttttgaggtg cgccagcagt aatgacaaca aggtcagcat
     9421 ctgcgcagtc tgcataagta gctgcgtaga tttttttagg tgatgtaaat gcaagggcat
     9481 gtgaaagatc ttcagcatca ccaacagctt tatcaaataa agctgggatt tcaatgatac
     9541 ctaactcttg cgcaatacct tggttaacaa gtgcaaaagc gtaagaagaa cctacggcac
     9601 catcaccaac gagaataact tttttatgtt gtttagttgc agtcatttct aaacatctcc
     9661 ttaaatattt ttgggaagtt tcccatacac ctatattcta tcattttcaa tgaaagaagt
     9721 cactaataaa atgtgaaaaa atttgcaaac aaattgaaat atcatagaaa actattgaaa
     9781 acgctgagtg tcttgatatt tttgataccg atttatcatc gcaattttac ctaaattatg
     9841 gtacaatgta agagacttac agtgaagaaa aggggcattt tttaatgcaa gataaaaatt
     9901 tagtagatgt taatctaact agtgaaatga aaacgagttt tatcgattac gccatgagtg
     9961 tcattgttgc tcgtgcactt ccagatgtta gagatggttt aaaacctgtt catcgtcgta
    10021 ttttatatgg tatgaatgaa ctaggtgtga cacctgataa acctcataag aaatcagcac
    10081 gtattactgg tgatgttatg ggtaaatacc atccacatgg tgattcatct atttacgaag
    10141 caatggtgcg tatggcacaa tggtggtcat atcgtcatat gctcgttgat ggtcatggaa
    10201 actttggttc aatggatggg gatggtgctg ccgcacagcg ttatacagaa gcacgtatga
    10261 gtaaaattgc tcttgagatg cttcgtgata tcaataaaaa tacagttgat tttcaagata
    10321 actatgatgg cagtgaacgt gaacctcttg ttttaccagc gcgtttccct aatttattag
    10381 tcaatggtgc aactggtatt gctgtaggta tggcaacaaa tattccacca cataatctag
    10441 gtgagtctat agatgctgtc aaattggtta tggataaccc tgatgttact acacgggagt
    10501 taatggaagt tattcctggt ccagattttc ctactggtgc cttggtgatg ggacgttcag
    10561 gtattcaccg tgcctatgaa actgggaagg gatcaattgt cttacgttca cggactgaaa
    10621 ttgaaacaac ttcaaatggg aaagagcgta ttgttgtcac agagtttcca tatggcgtta
    10681 ataaaacaaa agtacacgag catattgtac gtttagcaca agaaaagcgc attgaaggta
    10741 ttactgctgt tcgtgatgaa tcaagtcgtg aaggcgttcg ttttgttatt gaagttcgtc
    10801 gtgatgcttc agcaaatgtg attttaaata atcttttcaa attaactagc ttgcagacaa
    10861 attttagctt caatatgctt gctattgaaa aaggtgttcc aaaaattttg tcattacgac
    10921 aaatcattga taactatatt gaacaccaaa aggaagttat tgttcgtcgt actcagtttg
    10981 acaaagctaa agcagaagcc cgtgctcata tcttagaagg tttacttgtg gcgcttgatc
    11041 acttggatga agttatcaca attattcgta atagtgagac agacaccatt gctcaagcag
    11101 agttgatgtc acgttttgaa ttatcggagc gtcagagtca agctatttta gatatgcgtc
    11161 ttcgtcgttt aactggatta gaacgcgata aaatccaatc agaatacaat gacttactag
    11221 ctttaattgc tgatttagca gatatcttag ctaagcctga acgtgttgtt accattataa
    11281 aagaagagat ggatgaggtt aagcgtaagt atgctgatgc ccgtcgtaca gaattaatga
    11341 ttggtgaagt tttatcactt gaagatgaag accttattga ggaagaagat gttcttatta
    11401 ctctctcaaa caagggatat atcaagcgtc tcgctcaaga tgagtttaga gcacaaaaac
    11461 gtggaggacg cggtattcaa gggactggag ttaataacga tgattttgtt cgtgaattgg
    11521 tttcaacaag tactcacgat actgtgcttt tctttacaaa tttagggcgt gtgtatcgtt
    11581 tgaaagcata tgaaattcca gaatatggac gtacagctaa aggcctccct atcgttaacc
    11641 ttttaaaact tgacgaaggt gagacaattc aaacaattat taacgctaga aaagaagatg
    11701 tcgctaataa atatttcttt ttcacaactc agcaaggtat agtgaaacgt acaagtgttt
    11761 ctgaatttag taacattcgt caaaatggac ttcgtgctat taatttgaaa gagaatgacg
    11821 agttgattaa cgtcctcttg attgacgaaa atgaagatgt tattatcggg acacgtacag
    11881 gctactctgt tcgcttcaaa gtgaacgctg tacgtaatat gggacgcaca gcgactgggg
    11941 tacgaggcgt taatcttcgt gaaggtgata aagttgtggg agcttctcgt atcgttaatg
    12001 gacaagaagt actaatcatc actgaaaaag gttatggcaa gagaacagaa gcttcggaat
    12061 atcctacaaa aggccgtggt ggtaaaggaa taaaaaccgc taatattact gctaaaaatg
    12121 ggccattagc acgcttagtg acgattaatg gaaatgagga catcatggtc attacagata
    12181 caggtgttat tattcgaacc aatgttgcta atatttctca aactggtcgt tctaccatgg
    12241 gtgttaaagt gatgagactt gatcaagaag ctaaaatcgt cactgttgcg ctagtggaac
    12301 aagaaattga agataagtct aatatagagg atacaaaaga ataggaagtt atgcgtaata
    12361 aaaaaaaatc acatggtttc tttaactttg ttagatggtt attggttgta ttgctaatta
    12421 ttgttgggtt ggctttggtg ttcaataaac caattcgcaa tgctttcata gctcatcaat
    12481 caaatcatta tcaaatttca cgagttagta aaaaaacaat tgaaaaaaat aagaaatcta
    12541 aaacaagtta tgacttttca tcagtcaaat ctattagtac agaatcgatt ttatctgctc
    12601 aaacgaaatc acataatttg cctgtaatag gagggattgc tataccagat gtcgaaatca
    12661 atctcccaat tttcaaagga ctgggcaata cagagttgag ttatggtgca ggaacgatga
    12721 aagaaaacca gattatggga ggcccaaata actatgcctt agctagtcac catgtctttg
    12781 gtttgacagg ttcttctaag atgctctttt cacctttaga acatgctaag aaaggtatga
    12841 aagtttacct cactgataaa agcaaagtat acacttacac tattactgaa atttcaaagg
    12901 taacaccaga acatgttgag gttattgatg atacacctgg caagagccaa ctcactttag
    12961 tgacctgtac ggatccagaa gccacagaac gtattattgt gcatgctgaa ttagaaaaaa
    13021 caggtgaatt ttcaacagca gatgagtcga tattaaaagc attttcgaaa aaatataatc
    13081 aaattaatct ctagaaggta gttagaatta tgaaattaaa ggctgttcat catattgcga
    13141 taattgtttc tgactatgaa aagtcaaaag acttctatgt taataagcta ggatttgaaa
    13201 ttattcgtga aaaccatcgt ccggagcgtc atgattataa attagattta cggtgcggtg
    13261 atattgagct tgaaatattt ggaaatcgtc ttgatgatcc agaatatgaa actcctcctc
    13321 agagaatagg taggccgaat tggccacgtg aagcatgtgg tttacgacac cttgcctttt
    13381 acgttccaga tgtggaagcg tacaaggtag aactggagaa tttaggaatt tttgtagagc
    13441 ctattcgtta cgatgactat actggcaaaa agatgacctt tttctttgat ccagatggtt
    13501 tacctttaga attacatgaa taataaacaa agttgcgtgg atacgcaact ttgtttaatt
    13561 tactgaataa ttttatattc aagaagtttt ttaaagaaat aagtaagtta atcatggtat
    13621 aatgtaaggt atgaaaagaa ttgaaaagga attattttga aatgaggata ctaaaatgag
    13681 tattaaaaag ctattgtttg caggccttgc ttttatttta tttaccctag cgagtccagc
    13741 atatgctgca tcagatgttc agaaagttat tgatgaaact tatgttcaac ctgattatgt
    13801 cttaggttat tcgctaaatc aagaacagcg tgcacagaca ttacaacttc tgaattatga
    13861 tgaatctaga gacacgaaag ttaaaacgct aaatactagc tcatatgcta aaataatgaa
    13921 tatagctgat gatgcaagca tccaattata ttcatctgtt aaaataaaaa aattagggtc
    13981 taacgatacc ttagcagtaa acattgttac acctgagaat attactaaag ttacagaaga
    14041 tatgtatcgc aatgctgcag ttacattagg tattgagcat gcaacgatat cagtggctgc
    14101 tccaataaaa gtaactgggg agagtgctct tgcaggtatc tattattcat tagagaaaaa
    14161 tggagcaagt gtatcatcag aaaataaaca actagcacaa gaagagttaa gtactttatc
    14221 tggtattaat gctgagaata agggtaagga aggttatgac gccgacaagc ttaatgtagc
    14281 tttaacggat attaagtcag ccgtagctaa aggaggatct gatctttcaa aagatgatat
    14341 tcgaaaaatt gttgaggaaa cattgaaaaa ttatcatctt gataacgctg taacggaaaa
    14401 tcaaataaat ttaattgtta attttgcagt taatctttca caaagtaatg tcatcaaaaa
    14461 tagtgatttc acgaacacct tgaataattt gaaagacaac attgtttcga aagcaggatc
    14521 taaatttaaa aatatcaatg tcaattttaa tgctaataaa gctgttgaat caggtaaggg
    14581 atttttagct aatatttggc aacaaattgt taattttttc cagcagtttg gtagttaata
    14641 aaaaagtcta gcttataagc tagacttttt ttaccctaaa taaatgtttg tgttttttta
    14701 ctaatcttta aatgaatatt taagaataaa tggcgcaata atagttgaca gtataacaac
    14761 aataacaagc tcggaataag tagtgcttga aataatttta gcagctaatc caacttgaac
    14821 tataatgaga gccatctcgc cacgactgac cattccacca ccaattgtta aagattctaa
    14881 ttttgaaaaa ttaaagcctc gaccgacaaa gtaccctgga attaattttg ataaaacagc
    14941 caaagcagta aagattaata tcgtgtgaag gtgagacatc attccatcga atttaagagg
    15001 tagtgcaatt gaagcgaaga aaattgggat aaagaaggtg taactaagaa gtgaaatttt
    15061 atgttctacc ttatcaacaa aagaagtttg tccaatagct aatcctgcaa aaaacgaacc
    15121 aatgatagaa ctcattccaa ctttatcggc taaaattgat agagaaaaac agataaggat
    15181 agcaagaata gtatatttct caaagaaatg aaccttttcg attaatttga aaagagcagg
    15241 tacaaggtat ttcatacaaa taaataagaa aacaaagaat aacaattgaa tgataatttg
    15301 taatccgata ttagaagagc ttccatttgt tgcaataaag acactcagaa gcaaaacagc
    15361 taagacatca tcagcaacag cagcacctaa aataatagca ccagtatctg tttttacacg
    15421 attgtattct tgtaaaactt caactgtgat agaaatacta gttgcagcaa atacgagccc
    15481 gtaaaagata gcagtattga tttggaaccc aaacaagcgt gtcaagaagt agaagagagc
    15541 cataggaaca ataacaccag taattgcgac cagtagactg ggcttaagat acttctttaa
    15601 aagatcaaaa ttagcttcaa gacccgctaa aaacatgagt aagataacac caatttctga
    15661 caggacatgt aatacctgat tttgatgaac aagtcctagc attgcaggtc cgatgatgat
    15721 acctacaagt agttgtccaa caacagccgg tattccaatt ctttttgaaa taagagttgc
    15781 aagaacgctt gccaacaaaa tgattgtaat ttgaatgata atatgcattt tttcctccta
    15841 atattaataa aatgatatca atcatattgt actgaatatt tagaatacca ttttttaagc
    15901 ctaaaatcta ggaatatttc ttttctgtct tttt
//
LOCUS       DATRNX010000042        15893 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRNX010000042 DATRNX010000000
VERSION     DATRNX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15893
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            113..1396
                     /locus_tag="VBC81_001066"
     CDS             113..1396
                     /locus_tag="VBC81_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6912115.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
     gene            1551..1790
                     /locus_tag="VBC81_001067"
     CDS             1551..1790
                     /locus_tag="VBC81_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912116.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            1890..2423
                     /locus_tag="VBC81_001068"
     CDS             1890..2423
                     /locus_tag="VBC81_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6912117.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(2499..2993)
                     /gene="tpx"
                     /locus_tag="VBC81_001069"
     CDS             complement(2499..2993)
                     /gene="tpx"
                     /locus_tag="VBC81_001069"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6912118.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(3066..3725)
                     /locus_tag="VBC81_001070"
     CDS             complement(3066..3725)
                     /locus_tag="VBC81_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6912119.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(3725..4489)
                     /locus_tag="VBC81_001071"
     CDS             complement(3725..4489)
                     /locus_tag="VBC81_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6912120.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            complement(4494..5135)
                     /locus_tag="VBC81_001072"
     CDS             complement(4494..5135)
                     /locus_tag="VBC81_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6912121.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(5299..5850)
                     /locus_tag="VBC81_001073"
     CDS             complement(5299..5850)
                     /locus_tag="VBC81_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6912122.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(5872..6069)
                     /locus_tag="VBC81_001074"
     CDS             complement(5872..6069)
                     /locus_tag="VBC81_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6912123.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(6116..6658)
                     /locus_tag="VBC81_001075"
     CDS             complement(6116..6658)
                     /locus_tag="VBC81_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6912124.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(6714..6908)
                     /locus_tag="VBC81_001076"
     CDS             complement(6714..6908)
                     /locus_tag="VBC81_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6912125.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(6918..7499)
                     /gene="amaP"
                     /locus_tag="VBC81_001077"
     CDS             complement(6918..7499)
                     /gene="amaP"
                     /locus_tag="VBC81_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6912126.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(7514..7762)
                     /locus_tag="VBC81_001078"
     CDS             complement(7514..7762)
                     /locus_tag="VBC81_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6912127.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(7810..8055)
                     /locus_tag="VBC81_001079"
     CDS             complement(7810..8055)
                     /locus_tag="VBC81_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6912128.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            complement(8467..10746)
                     /gene="pcrA"
                     /locus_tag="VBC81_001080"
     CDS             complement(8467..10746)
                     /gene="pcrA"
                     /locus_tag="VBC81_001080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6912129.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(10852..11238)
                     /locus_tag="VBC81_001081"
     CDS             complement(10852..11238)
                     /locus_tag="VBC81_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6912130.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            complement(11371..12696)
                     /locus_tag="VBC81_001082"
     CDS             complement(11371..12696)
                     /locus_tag="VBC81_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6912131.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     regulatory      13004..13136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            13293..14639
                     /locus_tag="VBC81_001083"
     CDS             13293..14639
                     /locus_tag="VBC81_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6912132.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     gene            14703..>15893
                     /locus_tag="VBC81_001084"
     CDS             14703..>15893
                     /locus_tag="VBC81_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6912133.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKS"
BASE COUNT         5197 a         3076 c         2401 g         5219 t
ORIGIN      
        1 tttattttag gataactaaa caatttattg tttaaaatga aaggatttat gaatgtctag
       61 gaaaacattt aaacgtatac tatcgattgg agtttgcacg ctcgtactat cgatgagtct
      121 ttattacact gaaaaagccc acgctattgc tggtcctagt gaccgccaat acgtagaaaa
      181 cccaaatcct cacattattg taaatgttac aggtactgat caaaacggaa atagcatttt
      241 accgcattat atcgaagtca atgtaaagat gggacaaact ttaagtaaag aagaaattct
      301 agattatatt gctcgaaatt taaactctag tgttggagga gaaagtaaaa acgttcaata
      361 cagcaacatc gagtttaagg aaagtgctta tctgaagcgt caattagatg atggcaagac
      421 agaagaaata gcaattgata acgacggtgt tactgtacct aaagacggtc caaacaaatt
      481 ttggattgac gttccagtaa cttgtactgt tactcctatc gtaacagaaa cacatgaagt
      541 tcgatggggg actccagtcg ctatatcaca ccgtatttac tttgttgaag aatcttctgg
      601 aaaagtttta gatgaataca caaatctaca cactgctgat tcggaactta acggttatcg
      661 tgttggagat tatatcacag actatgcact ttctaagtct gcttacgaag cttttttaaa
      721 ctctcgttta gataaagaag gttacaaact tcaacatcgt attagcacga atgtacgaca
      781 aaaccttcaa attaataaat tgattttcaa ttatgacttt aatgaggaaa atatttacta
      841 ccaaatcggg aatatccgtc cactattaag tcgctcatca gctgaagtag aatctgacat
      901 cattacagaa cgctactatg tttctaaaaa tgctaaaagt ttagcacgta cagaatcaac
      961 catttcgatt aaaatggttg atgccaaaac tgaacaaccg ctatttaacc acacattaac
     1021 tggttatcaa ttggtaactg tctcccatgt ctataacaga ctctttgaag aaaatcttat
     1081 cccaactaca aaatcaggag aaagatattt tattcaaaat atgaaaaaaa cagctgaaca
     1141 agaatatact gtttatcttt cagaaacacc ttattctaaa gagaacgctc ccgtaatttc
     1201 ttatgatgca agacctgttg attgggatta tcactcaggc gcttcaggat cacttgaaaa
     1261 tcagcctaac atctatactg aagaagattc aactgaattt ttgggtaata aaccacaagc
     1321 agcttgttat ccaaacaaac aatttgcttg cgaaaatact gactctaaat acaactatag
     1381 ctatttagaa aaatagttat aaccgatagg gttaatctct cattatctta tactgattaa
     1441 aatcattaaa cttgtttcca ttttgactgg aaacaagttt tttatattta tttgtcacat
     1501 ataattgaca ctcttcgaaa aatgatatat acttaaaatg tcatctatat atgacacttt
     1561 tcttaaattc tttactaaag gagctaataa tcgtgaatcg tcttagagaa tacagaatta
     1621 aagctagcat atctcaatta gctttagcaa aatcagttgg tgtcgccaga caaacaataa
     1681 accttattga aaataataaa tacaatcctt cgctagatct ttgtataaga ctagcggaat
     1741 gcctacaaac agacctcaat actctttttt gggatagtag acatctataa cagattagac
     1801 tctctaaatc tatgaaaact cgggaggtta ggtcgtatta ttatttcaat ctcaaacttt
     1861 attaaatagt tcaaggatag gagaatatta tgaaagaaaa gaaaaatttg gggataaaat
     1921 taataaaatc cctatatggt attaatggcc cattcgatga atacagagaa caaaaaatca
     1981 attaccttgg aaatctatta tttgcaacca ctagtctcta tcacatttta tcctctatca
     2041 tcgcctggtt cacggtaagt aaatatccca cagagactgc atgtactctc ctagctttaa
     2101 cattctttct atatttttgt atttttctaa ttgttattcc ctcttatctc aacagtcatg
     2161 atttaataac gccactagaa gtctcaaaaa atgactatcc agtagcaata aagaaaaaca
     2221 aacaaaaatt tatcaaaatc gggatatttt atactgtcat ccagtggctt atcttgggtt
     2281 taacaactaa caataacact ccgctgattg tatcactatt ttctttaaaa gttattttgt
     2341 ctagtattat gtccggtgta gcttacaatg gagttatgta tctaatattt aaaaacaatg
     2401 ttgatattga aaaagatgaa taatgacaac atttcatctc taattgttct cactgtctat
     2461 ataaataata aggctggaaa attttccagc cttattattt atggtaatat ctttgcagca
     2521 ttaatcgccg cttcgtagtc aacatctgaa ttaacattgt caacgtattc ggtgtaagtc
     2581 accttattat gctcatccaa aataagaact gcacgcgtta acaaatgcca ctcattcatc
     2641 aaaagagcgt actcttgacc aaacgaatga tcataaaaat ctgataataa aatcacgtta
     2701 tcaagacctt tagcgctgca ccaacgcttc tgagcaaatg gaaggtccat agaaacagta
     2761 ataaccactg tgttatctag ctctgataat tcctcattaa aagtacgtgt ttgttttgaa
     2821 caaatacctg tatcaataga aggaacaacg ctaataactt tctttttccc ttcaaaatct
     2881 tttaaagatt tttgagataa gtctgtagct attagtaaaa aatcttttgc aatatcccca
     2941 acttgtagct gttttcctgt aaaagtaaca ggatttccaa gaaatgtcgt cataagttta
     3001 tctccttatc aattaccttt attttactat gaagcttctt ctttttcgaa taattgaact
     3061 gataattaga ggtacttact tccttcgcgt atgctcaaag tcgacaactc atcataatga
     3121 ttactgataa agtcctttac ccatacttta ttatactttg aataatccct aagcgcccag
     3181 ccaatcgcct tattgataaa aaattcttga cttcctgtat ttcttaaaat aaaaagtgac
     3241 aaaatatcag tatttgtttt ttctttaaat cctagttgat gctcaatagc tagtcgcctc
     3301 atccaaaaat cttgatcaag tgaccattct gacagtaaat tcataacctt gtcatctttt
     3361 aaggataagt tcccaaaaat cttagtcaga acatcaatac tatcccacca ctgttgatga
     3421 cgagcgtaaa actctagtct agagcaatca ttataggtga gaaattgact catagctaat
     3481 agataatcaa gtacaaaatg atgatattca cgatagtcac tttgccaaca tttatcaagc
     3541 aattgccaat caatttttgc ttgtctttta gcggccttta taagctcttt gtagagcata
     3601 cgtcgctcag gtgatttaat accatagcac ttaaaatgat ggcgtaaata agcctcctgt
     3661 tgcttagaaa cttctttgtc acttgcagct ttaaatttcc tctctaaact taggaataaa
     3721 tccattacgt aatccccttg tatttttcaa aaagcattgt aattccttca tgtacagatt
     3781 ctcttgatac acgtaaatta gcgtaaggag tcagttcatc aaaatcccct acttgaacac
     3841 tataatgcgc agcatcaaac atagaaatat catttttatc attgccaaaa gctataaaac
     3901 gattcccaaa taatttctta gtggcttttc ccttgtcaac tgcaatggga tttatgtaca
     3961 aacatttttc aaattcgtgg aaaaagtgac tactaagtcc aaaacgattt aatttcgcta
     4021 acatttggtc tgcccttctc agctgatcac caaaatacaa caccattttt atcggtttct
     4081 caattttaga aacttctaga agctctcccc ttttttgagg atcaacataa gcaataaagg
     4141 gaattttaga agcctgataa gtagcgtaat taaagacctc atcaacaaaa tatggaatct
     4201 gatagcgatg acaatatgaa ataatagtac taaaaaagtc actttgtaaa tagtaactat
     4261 ccactaagtg accattttca tgtaaagtcg caccatttaa accaataatt tttgataaag
     4321 atagcttgtc gccaaggata cctatagtgt ctctataaga tcgcgccgta gcaaaagtga
     4381 ctctatgacc ataatcaatc tgggctctct ctagtattcc ttgtatttct ttagatagac
     4441 tcataccatc aaaacaaaga gttccatcta tatcaaagac aaaatccatt ctctcaaagc
     4501 cccatcctta agttaaaata ggttttcata cgtgcataaa catcttcatc accaacaaaa
     4561 aagatatgat ctccttgctc tataactgag aaaggccctg gagaaacaat aaatttccct
     4621 tcgtgttcaa tagccacaat agtcgcacca gtttgatgcc atacatttaa ttctcctatt
     4681 gacttaccaa ggtgttcaga atcttcactg acaataattt catatggtgc tagtgggtat
     4741 tgtttactaa ccgcacgtgt ttgaagcaaa aaatcatcaa ctaaagtagc taactcttcc
     4801 atttcttgtt gctgagcctt aatattatca cggattttac ctttcaaaat agcaactgaa
     4861 tgagaggttt catattggtt taagaatgca attgcttttt ctttagaaag aataatggct
     4921 ccactgccat gctttaaagt taaaatctgt aaatctgcta aaatattaag acccttacgt
     4981 gctgtttctg gtgatacgtt gaatgttgaa gcaatagttg ttctagattt caacttctct
     5041 ccaacttcat aatctccatt tgcgattctt tgtgcaaccg ctactgctat tttttgatat
     5101 ttagatgtta caatttcaga ttgttcagaa accatccaac cctccttatc ctaataaaaa
     5161 catacatatt aaaccacaat tagcaaaata ttgcaaatat atcttttttg ttaatatcta
     5221 atgtctttag taaatatcat ataaaaggag ctggtctccc agctccgagt gtcttatcat
     5281 aaaaagtagt ttactgaatt aagattcact agtacgagct tctgagattt tttcttttgc
     5341 agcctcagta ccatttgata ctgcttcttt tgttttctct gctccagaag aaattgctgc
     5401 tttagctttg ccagcttgtt cagaagcaaa gtttcctgtt gcttgtgctg catcagatac
     5461 acgatcttgt actgtcactg agtcagcttc atgttcagct ttagtcttaa catctacgac
     5521 atttgcgttc aattcaacaa ctttaaggtg ggtcatcact tcaacgtttt gactgacaat
     5581 cgctttaatg ctttcaacga tagctgggat atccttgccg tattcaacaa cgatgtctaa
     5641 atcaactgca acttctttag taccaacttc aacatttaca ccatcagtta ctgagtcaga
     5701 atttacaacg ctatttttta gattagagaa gaaacctcca ctcacagcca agagtccgtc
     5761 tactgattct aaagcatgtc caacaatttt agcaattacc ttttcttcat aagttactga
     5821 agttttaata tcttgttttt gattttccat aaataaatga cctcaaacct tctagtcctt
     5881 tttcttaaag ctctctgaag cacccttaat ggtatcttta atgtcagcaa gtttttcttt
     5941 tgcatgacct ttcaatttat cgactttacc ttcagattca acttctttat cgccagtaag
     6001 tttcccaaca gattctttta ctgaaccaga aaccttatca acttttgcat caaatttttc
     6061 ttcagacata atagcctcct actaataaca atactttaaa ctatacttaa atcaattatt
     6121 gtacacgtgg ttcagacttc atgtcttcca ctttatcaac accgttacct acggcacttt
     6181 taacgtttga aacttgattc gaagcaaatt cacctgttgc ttgtgctgca cttgttacac
     6241 gatcttgaag agttactgaa tcttcttcat gttgagcacg agttttaata tcaacaacat
     6301 taacattaac ttcaaccact tcaaggtctg tcatacgttt cacttcagct tcaacaactt
     6361 ttttgatatc ggcaaagata gttggaacat gtttttggta ttcagcaaca atatccaaat
     6421 caacagcaac ttgcttttta cccacttcaa catttacgcc atctgtaact gaatcagaat
     6481 ttacaacgct attttttaaa tttgagaaaa aaccaccatt tacagccaaa agaccatcta
     6541 catgctcaat cgcaattcca acaatttttt caatcacttt atcttcgaaa gttaattctc
     6601 cacgaacagc tgtcgtgcca gaattgttag ttgtgttttt gatgtaagtt tcagtcataa
     6661 tagttcctct ttcttttatt taaataatgt agaataaata taaacgaaaa attttatttt
     6721 ctcttattaa agaattggtc aagcatacct gttctttgca cataaagccc agcataagca
     6781 cctagcacaa tgataacaag agcaagaatt gttttaaaga atccaatggt tacaatcatt
     6841 gcagctaaaa cgagcccaat aactgcacct cctagtggat atctgtattt cctcacgaat
     6901 tctgacatat tagactccta ttctacacga cttttattcg tcttagcatt tccaacattt
     6961 tcttcttcaa cttgcttaac aaatactcta gtgttcattt tcttttccaa cccaaagaat
     7021 tcttttaagc caagttcaac atcactttga atatgttcaa gttgttccgg tacatttgta
     7081 cgagaagtta aaagaccttt aacatcaact ttcacttttt tcttatataa atgagctgtc
     7141 acactaggag atttcatcaa accagttttt aatacttctg ttttaacaaa tccctctatt
     7201 gcagattttt ttaacatcaa tttaccagtt ttatcagcca gtttatactc tgtgtatcgt
     7261 cttggataaa acagaactac caatatagcc agtaaaacga ttacaaacag cactaatgct
     7321 ccccaaaaga aataatgata cataccaacg tcaatcggtg atggcaaatt aaaatcccaa
     7381 tctaaccaat taaaagatgg tagataaatg tgttgtttac tgataacagc tacaaatcct
     7441 agtagggtaa gggagattag acctagcaaa gtatataaac tttttagtcc ttttgacata
     7501 ggtaactcct tttctagtta ttcattttac ctagtacaaa tgaagtaaca atgacaacaa
     7561 taatcgcacc tacgattgaa ggaatcaaag ccatacctgc taatttaggc ccccatgttc
     7621 ctagtaaact ttgaccaaca aatgaaccaa ctaaaccagc taagatgtta gctatccaac
     7681 ccattgaacc acctttgtta gtaacagccc ctgcaatagc accgataata gcacctacga
     7741 ttaatgacca aattaaactc atgataaata ccttctcttt cttgactaat cattctacta
     7801 gttatgtagt tatgattttt tgcctaatac aaatgaagta acaatgataa cgataatcgc
     7861 acctacgatt gaaggaatca aagccatatc tgctaactta ggcccccatg ttcctagtaa
     7921 actttgacca acaaatgaac caactaaacc agctaagatg ttagctatcc aacccattga
     7981 accacctttg ttagtgattg ctcctgcaat agcaccaata atagcaccaa cgattaatga
     8041 ccaaattaac cccatgataa atacctttct tcctaagtac atctcagtgt acaaacttat
     8101 tttgttctta actaaataac aagagtttct aaagtgacca ctcggcaatt aattaagttg
     8161 ttaactatac taatattata caactttata aagtgactgc caagtaaaaa gcttggtaaa
     8221 tgcttttatt tttaagtgac cacttgatat ttaattgata ttgttattat actcttttta
     8281 gaaaagaatg caagagaaac gcttctttct ttaaaatagt atggtcactt ttggaaaacg
     8341 ctttaatatc aaccttttta gtaagtaaaa accaaatgat aacttatttt gtctattcaa
     8401 atgatgcaaa aagactaaga atacaattct tagtctctga ttatttaagc atataataga
     8461 agaaagttag ttttcctttt tactaatagg agcgacactc gctaataatt tctttaaccc
     8521 aatacctgga aaatttattt ttaactcttg agtctttccg ctacctgaaa cttcaagcac
     8581 cgtaccgtcc ccccattttt tatgagttgc aacatcacca atctcccatg atgtctcatg
     8641 gctattgttc gcgttaagag cctggagttg ggcagttact tgaggttgtg aattactttt
     8701 acgtgcctga agcgcttgtt gaagactcat tccttgacca aattgtgtag gctgttcttt
     8761 tgaatatttc acaccaaatg aagaattaac tggtcgagcc aacccttgat gttgtatcag
     8821 ctcatcatca atctcacgta taaaacgggt tggtctatta taactagttt taccaaataa
     8881 agtacgggta ttagcattgg taaggaaaag tatttgttct gctctcgtaa tcccgacata
     8941 agctaaacga cgctcttctt ctaattcatc agcatcctct attgcacgcg ataaaggaaa
     9001 tactccttct tccataccga ttaagaaaac aactggaaat tctagaccct tggcagcatg
     9061 taatgtcatt aaagtgactt cagccgtttc agtggcgcta tcatcagtat ctgctattaa
     9121 agctaaatca tttaaaaaac gcgatagacg atctaaacca gtttcaccct ctactgtaat
     9181 ctctgggtta tcgtcaaaat ttttagtcac tgataagaat tcttcaatat tttcaatacg
     9241 cgcttgactt tctaatgtat tttgaacttg aagtgcttct agatatcctg tcttatccag
     9301 taggttttct gtaatctccg ttacagttaa actatctagc ttactacgta gagtcaaaat
     9361 cagattagct aaatcccaaa cagcttgagc agccttccct tttaagggcg atatcatgac
     9421 atttgaagag gaatcaagaa gagacatgtt ctgttcatag gcaaatgacc gtattttttc
     9481 taaagttcct ggccccaccc ctcttttagg ttcatttaca atccgctcaa aagaaatatt
     9541 atcagaagta tttgcaagga tattgagata agcaataaca tctcgaattt ctttacgact
     9601 atagaatttt gttccaccaa ccattgtata tggaatattg gattttaaaa gtgcttcctc
     9661 aatagtacga gattgcgcat tcgtacgata aagaacggca aaatctttga aattttttcc
     9721 ttgttctcga acgatgttat caatagttga cgctacaaaa acggcttctt cttgctcgtt
     9781 gttagctcta tgatatacaa tttgctcacc ttcatcattt tgagtccata attttttagg
     9841 acggcgattt ttattatgat tgatcacatt attagcagct tgaagtattt tcttagttga
     9901 tcgataattt tcttctaata atacaacttt ggcttgcgga tagtcctttt caaaatcaag
     9961 aatattttgc atatcagctc cacgccatcc ataaatggat tgatcggcat caccaacaac
    10021 acaaatattt ttgaaacgcg aagctaataa cttaactaat tggtattgag cgtggttagt
    10081 atcttgatac tcgtctacat ggatatattg atacctctgt tggtaatagg ccaaaacatc
    10141 tttattctga tcaaataatc gaagtgtcat cataatcaag tcatcgaaat ccatggcctc
    10201 acttctacgt aattcctctt gataagcttt atagcattta gcaattacct gtgtatacat
    10261 atctccggct tgcttctcat atgcaatttc atcaagaagg tcatttttag cattagagat
    10321 tgtacctaaa attgaacgtt cattccactt ttttgtgtcc aaatttaatt gcttaatgat
    10381 tcgcttcatt aaagtacgct gctcacctgg atcaacgatt gtaaagttac gattatatcc
    10441 aatataatca gcttcacgac gtaaaatacg aacacacata ctatgaaaag ttgcaatcaa
    10501 agtatcttgg gtagctggat tcaaggcgat agcacgttca cgcatttcac gcgctgcttt
    10561 attagtaaaa gtaatcgcta aaatattcca aggattaaca tatttttcat ctattaaata
    10621 agcaatacga tgagtcagaa cacgtgtttt tccagagcca gctcctgcca taatcaaaag
    10681 cggtccgtct gtcgtttgta ccgcttctgc ttgtttatca ttcattccaa ttattaaagg
    10741 attcatttct actcctaatt ttgtcaaaat tgcctctcta gtatatcatt ttttcatctt
    10801 tttcccaata gaaaaaggag ttaaacagac gctgtctaac tcctaccttt tttacttctc
    10861 ttttctctct ccagttacaa acattgtaaa aatagcagtt gtaaccattt cgtctttctg
    10921 atttttaatg tgaacatcaa cgacttttgt agtacgacca ttatgaacac aagtaccgat
    10981 gacagtaagc agatcatcta atcggcctgc cctcaaatag ttgatatttg attgtagagt
    11041 taccgcttca tatcctgttg aaatagcaac taaaccactg acttgatcac acagtgtaaa
    11101 caaataacca ccatgagcat taccataata gttgagcgaa gagtcaacga ccttcgttgt
    11161 taaggtaact tgaccttctt cccagttttc aatttcgtaa ttctcaaata cttttacttg
    11221 ttctaaattg aatttcattc tactataacc tcactttttc tatgaccatt atagcagaaa
    11281 ataaggaaac cataactgta aacgttttta ttttaaagtt tttaactgat ctatcataac
    11341 aaaagagctc agatactctg agctcttttg ttatgatttc atgctttctt ttggcaatat
    11401 taaatttaat ataataccca ctatagcact taatgctgtt cctgataaag ttagagcacc
    11461 tagatcaagg actgctccac cgaggcctaa aactaacatg gaactggcaa taatcagatt
    11521 acgaacctca gcaaaattga cacgattttc aatcaacact tttaatccat tacttgcaat
    11581 aacaccataa agtaatatag ccattccacc aagtactgca ctaggaattg tagagatcaa
    11641 agctgtaaat ttaccaaaga atgaaaaagc gattgcaata aaagctgcat tacgaataac
    11701 cgttacagaa gctatacgcg tcatgccaat aacacctgta ttttcaccat aagtagtttc
    11761 agctggccct cctataagag cagaaaatgc ggttgctaaa ccatctccaa ttaaaagacg
    11821 atttaaacct ggttttttta gaaagttacg cccacaaatc tgacctaaaa ctgtatgatc
    11881 accgatattt tcagcaatcg ttacaattga gattggaaga attgcaagca tttctggtcc
    11941 aaagtaaagt ttataactgt gaaataaacc tgtcttaaac ggtaaataga attttggtaa
    12001 ttcaaaccag gcagcttttt caactggaga taaatctacc aagccaagaa tgattgataa
    12061 aatataacct ccaataattg caaataaaaa tggaataatc ttgataaaac ctttcccttt
    12121 agtattgata aaagctgcaa tcaagaatgt aacaacagca actagcattt tacgccaatc
    12181 acctttagcg acaaaacctg cattagttac agcagagttt gccaaaccta aaccaataac
    12241 aataatcata ggtccaatga ttattggtgg caaaatctta tcaacccatg aatttcctac
    12301 gaacttaata accgtcgcca caacgacata gattagacct acaaaaagaa tacctgtttg
    12361 agcagccgaa atatcgccat gcatttgttt catggctaat gccatagctg taatataagc
    12421 aaatgatgag cctagataaa cagggacttt aaattttgtc gcaacttgat aaatgagcgt
    12481 tccgcaacca gaagcaaata aagccactga tacaggcatc cctaaaataa gcggaactaa
    12541 tatcgtagct ccaaacatgg caaaaacatg ttgaaaactt aaaagcaaac cttgaaataa
    12601 ttctggtttt tcatcgatat ctagtaataa gttagtagaa tgtacttcct tatctactag
    12661 aatttgatta ctttcacctt tagcatttaa agtcatatct aaactagtcc tcttgggcat
    12721 acaaaaaagc ccactttcta tagcaatata gtcataggct ttattttttt cctgatgttt
    12781 ttctcacctc acgggatgag tttaaaaacc tacgctgaaa gaattatagc ataagacatt
    12841 ttagaaaaca agagtttttt gaaactatat tgacaaaact atgctttttc attatgatag
    12901 ttaacataat aaaatatacc gaatgacgtc attcaggaga agaaagttag actttcgccg
    12961 aaggaattac actctcaggt gtcttaagac aggactgatt gacagacgga cttctggaga
    13021 gacctataag tagcaacatc tttgtattga caccaagatg tgctctaggc gccgaagggg
    13081 caagaagagt aaaacaactc ctccaatctc tcaggcaaaa ggacagaagc taaaagccaa
    13141 tattaataat gagtagtaag cttattaagt ttactactac ctttatttgt gcgcttttta
    13201 gctagcatct ttcagaagtt atctctttta gagataactt ctttcgtttc attacagaat
    13261 ccataggtat gtcatgtatc aaaggagaac atatgctaac actttttact catatcaata
    13321 gcttcgtttg gggtccacct ttacttgctt tattagtcgg aacaggtatt tacctatcat
    13381 ttcgcttagg ttttgttcaa ttgagacaac tttctagagc tttcaaattg attttccgag
    13441 aagataatgg acaaggagat atttcaagtt atgctgctct tgcaactgct cttgctgcaa
    13501 cggtagggac aggtaatatc gttggtgtgg ctacggctat taaatctgga ggaccaggag
    13561 ctttgttttg gatgtgggta gccgcctttt ttggaatggc aacaaaatac gcagaaggat
    13621 tattagctat caaatatcgc acaaaagata ctaatggaga aatttctggg ggaccaatgt
    13681 actacattgt caatggaatg ggacaaaaat ggaaaccatt agcagtcttt ttttctgcag
    13741 caggtatctt agtagctctt ttaggaatag ggacattcac tcaagtcaat gcaattgcct
    13801 cttctcttga gcatactttc aaaatatcaa ctagatttac cagcctcatt cttgctgtta
    13861 tagtattatt tattatattt ggaggtataa aatctatttc aaaagtttca gaaaaaatag
    13921 tgccttttat ggctataagc tatattttag caactctgat tattattgca gttaattaca
    13981 ataagattcc acatactttc caactcatct ttagcggtgc tttttctggg acagcagcca
    14041 ttggtggttt ttctggagct atcgttaaag aagcaattca aaagggaatt gctagaggag
    14101 ttttctcaaa tgaatctgga ttgggttctg ctccaatagc tgcagcagca gctaaaacaa
    14161 aagaacctgt tgaacaaggg cttatttcaa tgacaggaac cttcattgac actattgtta
    14221 tttgtacttt gacagggatt gcaattttag ttacaggaaa atggctagaa tttgatttac
    14281 agggtgctcc gttaacacaa gcatctttta ataccgtatt tggctcacta ggtagttttg
    14341 ccctaacctt ctgccttgtc ttatttgctt ttactactat tttaggatgg agctactacg
    14401 gagagcgttg ctttgaatat ctattgggga ctaaatttat taacgcttat cgcattattt
    14461 ttgtcattat ggtcggctta ggtggtttct tacagctcga tctcatttgg gttatcgctg
    14521 atatcgttaa tggtttaatg gctttaccaa atctaatagc tctcctagct ttgtcaccaa
    14581 ttattgttaa agaaacacag aaatattttt cagagactaa aaatatcact aagccttgaa
    14641 cttactagtt ttatagtctg ctcctaagtg atataatgtg tagatgaaag gaaattagga
    14701 aaatggcaac ttctactgaa aacttacaac ttgcaaaaag agggcctatt attagtatca
    14761 ttgcctatat aacactggca gtcgctaaat tagctgcagg ttattggttt gatgctactt
    14821 cacttgtagc agatggattt aataacttat ctgatatctt aggaaatgta gcactcttaa
    14881 ttggtttaca cttggctagt caaccagccg actcaaacca tcgttttggt cactggaaaa
    14941 ttgaagattt agcaagcctg attacctctt ttatcatgtt tgtcgtaggt attcaagttt
    15001 ttattcaaac tgtcactaag attattaata atactgatac taacattgat cctctaggtg
    15061 ctatagtagg tgctatatct gctcttgtta tgcttggcgt ttgtttctat aataaacaat
    15121 tatctcaaag agtaaagtct agcgctctgg ttgcagcttc taaagataac ctttctgatg
    15181 ctgtgacatc tatcggcaca tctattgcta tcattgcggc ttctctaaat tttcctatta
    15241 ttgatcgttt agctgctatc attattactt attttattct taaaacagct tatgatattt
    15301 ttatagaatc tgctttcagc ttatcggatg gtttcgatga ttatcaatta aaacaatacg
    15361 aaaaagctat attaacaatt cctaaaatat ccgctgtaaa atctcaacgt ggacgtactt
    15421 acggtagtaa tatttacctt gatattgttc tcgaaatgaa ccctgatctc tcagtctttg
    15481 aaagtcatgc cattaccgaa aaagtggaaa aactactcag tgataaattt tctgtatatg
    15541 acatagatat tcatgtagaa cccgcttcta tcccagaaga cgaaattttc gataatgttt
    15601 accaaaaatt atacaaaaac gaaaaaatca tcctagcaaa aatccccggt tatgagactt
    15661 ttattagccc tgacttttat atgattaatg aaaagggaaa tattatcact tctgacatgc
    15721 ttactaatgc tacaaatcat tcattggcaa gcaactttaa atatttcaat gtaaaatcaa
    15781 ttagtcaaaa aacaaaactt gtgtcttatg aattggaagg taaacgacat actagtattt
    15841 ggcgccgtaa tgaaaaatgg ttcttaatct accatcaaat aactgccaag tca
//
LOCUS       DATRNX010000043        14821 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRNX010000043 DATRNX010000000
VERSION     DATRNX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            17..1171
                     /locus_tag="VBC81_001085"
     CDS             17..1171
                     /locus_tag="VBC81_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6912134.1"
                     /translation="MFFKWILNNQAVIALMITFLVFLTIFIFTKISFMFKPVFDFLAV
                     LILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLLIWAMSSFIPMMSNQ
                     LRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNISSQAVSYAEKFSKNV
                     LDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLPTKLRQPADRILREM
                     NSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVLNMVPYLGSFVAQIP
                     VFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHPITIMFILLTSGAMF
                     GVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSEEVKNVE"
     gene            1161..2387
                     /locus_tag="VBC81_001086"
     CDS             1161..2387
                     /locus_tag="VBC81_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912135.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2497..4179
                     /gene="alsS"
                     /locus_tag="VBC81_001087"
     CDS             2497..4179
                     /gene="alsS"
                     /locus_tag="VBC81_001087"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6912136.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4193..4912
                     /gene="budA"
                     /locus_tag="VBC81_001088"
     CDS             4193..4912
                     /gene="budA"
                     /locus_tag="VBC81_001088"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6912137.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4966..6621)
                     /locus_tag="VBC81_001089"
     CDS             complement(4966..6621)
                     /locus_tag="VBC81_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6912138.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            6980..7984
                     /locus_tag="VBC81_001090"
     CDS             6980..7984
                     /locus_tag="VBC81_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6912139.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            7997..8860
                     /locus_tag="VBC81_001091"
     CDS             7997..8860
                     /locus_tag="VBC81_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912140.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8860..9621
                     /locus_tag="VBC81_001092"
     CDS             8860..9621
                     /locus_tag="VBC81_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912141.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9918..11579
                     /locus_tag="VBC81_001093"
     CDS             9918..11579
                     /locus_tag="VBC81_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6912142.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11636..12424
                     /locus_tag="VBC81_001094"
     CDS             11636..12424
                     /locus_tag="VBC81_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6912143.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12514..13224
                     /locus_tag="VBC81_001095"
     CDS             12514..13224
                     /locus_tag="VBC81_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6912144.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLRNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13462..14133
                     /gene="rpiA"
                     /locus_tag="VBC81_001096"
     CDS             13462..14133
                     /gene="rpiA"
                     /locus_tag="VBC81_001096"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6912145.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14190..>14821
                     /locus_tag="VBC81_001097"
     CDS             14190..>14821
                     /locus_tag="VBC81_001097"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6912146.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVG"
BASE COUNT         4739 a         2288 c         3047 g         4747 t
ORIGIN      
        1 agaatttaaa aatagtttgt tttttaaatg gatcttaaat aatcaagcag ttattgctct
       61 catgattacc tttttggtat ttttaacgat ttttattttt accaaaatct cttttatgtt
      121 taaacctgtg tttgattttc ttgctgtgct gatattgccg cttgtaattt ctggcttgct
      181 ttattaccta ttaaaaccta tggttacatt tttagagaag cggggaatta agcgtgtaac
      241 agcgatatta tcagttttta ctattataat ccttctgtta atttgggcaa tgtctagttt
      301 tattcccatg atgagtaatc aattacgcca ttttatggaa gatctccctt catatgtgaa
      361 taaagtgcaa atggaaacaa gttcgtttat agatcacaac ccttggttaa aatcttataa
      421 aggggaaata tcgagcatgt tatctaatat cagtagccaa gcggtctctt atgctgaaaa
      481 attttcaaag aatgttttag attgggcagg aaatttagct agtacagttg cacgtgtgac
      541 agtagcaaca atcatggctc cctttatttt gttttatctt ttaagagata gtcgcaacat
      601 gaagaatggt ttcttaatgg ttttaccaac caaactacgc caaccagctg atcgtatttt
      661 gcgagaaatg aatagtcaaa tgtcaggata tgtgcaagga caaatcattg ttgctattac
      721 tgttggtgtt attttttcaa taatgtatag tattataggc cttagatatg gcgtgacatt
      781 agggattatt gccggtgtgt taaatatggt tccctatttg ggaagttttg tcgcccaaat
      841 tccagtgttt atcttagcgc ttgtcgcagg acctgttatg gttgttaaag ttgcgattgt
      901 ttttgttatt gagcaaactc tagagggacg ctttgtctca cctttggttt taggtaataa
      961 acttagcatt catccaatta caattatgtt tattttatta acctctggag cgatgtttgg
     1021 tgtttgggga gtattcctca gtattccgat ttatgcatct atcaaagttg ttgttaaaga
     1081 attgtttgat tggtacaaag ctgtcagtgg gctatataca atagatgttg ttactgaaga
     1141 aagaagtgaa gaagttaaaa atgttgaata gtgaaaaaat gattgtttct atccaaaatc
     1201 aggatttaga acatgctaac aagtatttcg aaaaagcgtt gaaaaatgat cctgaggaaa
     1261 tcttattgga actaggagct tatttggaga gtattggttt tttaccccaa gcaaaacgtc
     1321 tctatgatca aatccgacca aattatccag aagttgctat caatttagct caaattgttg
     1381 ccgaagatgg agatattgaa caagcattct tatacttaga ttatatctca gaagatagtc
     1441 aagagtatgt ttcagccctc ctcgttatgg ctgacttata tgatatggaa ggcttgactg
     1501 atgttgcgcg tgaaaagctt ttgctagcca gcaaacttag cgatgaccca ttggttacat
     1561 ttggtttagc tgaaatgaac cttagtttgg aacattatca agaagcgata gaaggatatg
     1621 catcacttga caatcgagaa atcttagaaa cgactggagt ttcaacttat cagcgtattg
     1681 gtaaatccta cgctattata gggaaatttg atgcagctat cgaattttta gaaaaagccg
     1741 ttgacattga gtatgatgat ttaacagttt ttgagttagc aacaatcctt tatgatcaag
     1801 aagaatatca aaaagctaac ctttatttta aacagttgga tactattaat ccagactttg
     1861 atggctatga atatatctat ggtttatctc ttcgtgagga gcataaatct gaagaagctt
     1921 tacgtctagt tcagcaaggt cttcgtaaaa atagttttga tggtcaactt ctcttactag
     1981 cttcacaatt atcgtatgaa ttacatgatg ttccttcgtc ggagtcttat ttgaaacaag
     2041 ctgaaaaagt ttctgaaaac caagatgaaa ttgttatgag attgtcaaat ttgtatcttg
     2101 aagaagagcg gtttgaggaa gtcttggaat tagacaatga tgatttagaa aatatccttg
     2161 caaaatggaa tatcgctaaa gcacacaagg ctttagaaat ggatgatagt gtagactatt
     2221 atcaatcttt gtataatgat ttaaaagata atccagaatt tttacaagat tatgcttaca
     2281 ttttgagaga atttggttac cttgataagg cacaagaagt aggaaaggct tatttaaaat
     2341 tagttccaga cgatatagag atgtcagaat gggtgaataa catttaaata ggaaaacact
     2401 tacagaaaaa aataaaaaat tcacaagtaa attttcacaa tctgatgtaa aaactgtgat
     2461 ataattgaac cattcttgtg aaaagaggga gataaaatga cagaatcaca taatcaatat
     2521 ggggctgact taattgtcga tagcctcatt aatcatgatg tcaaatatgt ttttggtatc
     2581 ccaggagcta agattgaccg cgtatttgac actctagaag ataaaggtcc agagttaatt
     2641 gttgctcgtc atgagcagaa cgcgactttt atggctcagg ctgtcgggcg tattacggga
     2701 gaaccaggag ttgttattgc aactagtgga cctgggatct cgaacttggc aacagggtta
     2761 gttaccgcga cagatgaagg tgatgctgtt cttgcaattg gtggacaggt taagcgagga
     2821 gatttattga aacgtgctca tcaatcaatg aataacgttg ctatgctaga acctattaca
     2881 aaatattctg ctgaggttca tgaccctaat actttgtcag aaactgttgc aaacgcctat
     2941 cgtcttgcta aatctggtaa accaggagca agttttatct ctattcctca agacgttaca
     3001 gacagtccag tctcagtaaa agcaatcaaa cctcttagtg cacctaaatt gggttcggca
     3061 tctgttttag atattaatta tttagcgcaa gctattaaca atgctgtttt accggtactg
     3121 ttattaggaa atggcgcatc ttcagaggga gtaactgctg ccgtacgtcg acttcttgat
     3181 gctgttaaac taccagtagt agaaactttc caaggtgcgg gtattgtgtc acgtgagctc
     3241 gaggatgaaa cattctttgg tcgtgtaggc ctcttccgta atcaacctgg agatatgctt
     3301 ttgaagcgtg cagacctagt tattgctatc gggtatgacc ctattgaata tgaggcgcgt
     3361 aactggaatg ctgaaatttc tgctcgtatt atcgttattg acgttgaaca agcagaaatt
     3421 gatacgtatt tccaacccga gcgagaactg attggggaca tggcacatac acttgattta
     3481 cttttaccag ctattaaagg atatgaattg ccagaaggtt caaaagagta tttgaaaggt
     3541 cttcgtaata acattgaaaa cgttagcgat gttaaatttg atcgtgactc agctcatgga
     3601 cttgttcatc ctcttgatct cattgatgta ctccaagata ataccaccga tgacatgact
     3661 gttacagttg atgtgggaag ccattatatt tggatggctc gctactttaa atcatatgaa
     3721 gcgcgtcact tgctcttctc aaacggaatg caaacattag gagttgctct accttgggca
     3781 atttctgcgg ctcttttgcg tcccaacaca aaagttattt cagtttctgg tgatggtggc
     3841 ttcttattct cagctcaaga gttagaaact gcagttcgtc ttcatctacc aattgttcac
     3901 attatttgga acgatggaaa atataacatg gttgaattcc aggaagaaat gaagtatggg
     3961 cgttcttcag gtgttgactt tggaccagtt gattttgtaa aatacgctga aagtttcggt
     4021 gctaaaggtt accgtgtaga cagcaaagac agttttgaag aaacactcaa acaagctcta
     4081 atcgatgctg aaaatggacc agtgttgatt gatgtgccta ttgactataa agataacgtg
     4141 acactaggtg aaacaatctt acctgacgag ttctactaag acgaggtcta atatgtcaga
     4201 aacagttaaa ttatttcaat acagcacctt gagttcactg atggcaggat tgtacaaagg
     4261 cagtctaact atcggagaat tattaactca cggtgactta ggaatcggta ctgttcatat
     4321 gattgatggt gaattgattg tattggatgg gaaagcttat caagctattg gtacagatgg
     4381 taaagcagag attatccaat tatcagatga tgtgacagta ccctatgcag cggttttacc
     4441 acaccatatc caaaaacaat ttgatatcaa tgctgagaca gataataagg atttggaaga
     4501 gatgattcta aaaaactttg aaggtcaaaa tctctttaag tctttaaaaa tcaaaggaac
     4561 gttttcaaga atgcacgttc gtatgattcc aaaatctcca caacataaac gttttgcaga
     4621 tattgcatct aatcagccag aatttaccag agagaatgta tctggtacgt tggttgggat
     4681 ttggacgccg gaattatttc atggtgtggg ggtaaaaggg ttccatgtcc actttatttc
     4741 agacgattta actttcggtg ggcatgttat ggattactcg ctcacacaag gaaaagttga
     4801 aattggaaag gttgaccaac ttgaccaatg tttcccaact caagatcaag aatttttgaa
     4861 agctaacttt gacctacaaa agcttagaga agatattgat ttaagtgaat aagaatacct
     4921 ataaataatt aataacacta aaaatagaca gggatatgtc ccagtctatt tttttagttt
     4981 aaggctatct attttttctt gtgtgggagt gactcgtaag gttttttgac cagtgtaagt
     5041 tacaaaacct ggcttagtac cacttggttt attgagtttt tttgcttcaa tcatgtctac
     5101 ttgaacaaga ttggagagac gagctttaga gtaataagca gctaactcgg cggcatctgt
     5161 cttaacctcg tcacttggat tgaggttatc ccgaattaac acatggcttc cggggatatc
     5221 cttggcatga aaccacaact cccctttacg agccatttta aatgttaact cgtcattttg
     5281 gagattatta cgtcccacca taataatagt cttgccatcg gatgctaaat attgttcagg
     5341 ttttttacgc ttatgttgtt tatcatgtgc gcgtcgctta ataaatccag tttcaacaag
     5401 ttcttcacga atatcattga tatcttccat agaagcatga tttagcgatg tttcaacaga
     5461 ttcaaggtag gtgattgtat ttttcgtatc tgaaataatt ccttttaaat gttttacagc
     5521 ttcttttaat ttttgatatt ttttaaagta gcgttgggca ttttggttgg gtgttaaagc
     5581 tcgatcaagt gaaatttcaa tcgtttggtt ggtgtaataa ttatcaagaa caacgatatc
     5641 ttgattattt ggcaccatag agagataggt agttagaagc tctccttttt ggcgaaactc
     5701 ctctgcattt tcagtagcta gaagttcgtc ttgttgctta gctagttttt tgatattttt
     5761 ctctaactca ctttgaacac ggtgaatgag gtcactagct tgctgtgcta tgcgatcttt
     5821 ttcagccttc tcttggtagt aataatctaa caattgtcca agagactgaa atgtttcgtg
     5881 actgcttgag aattggacag cggaaaacga tttttctgtt aggctaggat aatattctct
     5941 cgaaaagaat tgacgaaaat cgttaagttt attgtctttc aaacagtgag ataattctag
     6001 cgcagtatcg cgtcctaaac cttggaagag ctgttggagg tttttagggc ttaggtcatt
     6061 ggtttgaagc agctcaaata gagtttgatc agaaatatca aaaggattga ttgcttttgt
     6121 tttgggtggg gcaatgtaag ttgaaccagg aaggatagta cggtaggagt tttgcgagaa
     6181 accaacatgc ttaattgatt caataatttt atgttcattt ttatcaatca agataatgtt
     6241 actgtgtttt cccatgattt caacaacaag tgtcgcttta atgtgatcac cgatttcgtt
     6301 tttattagac acgatgattt ctaatatacg gtcattttca atttgttgaa tggtttcaat
     6361 cactgcacct tgcaaatact ttctcatgat cattgtaaaa gtatttggat tttgaggatt
     6421 ttggaaattt gcttcggtag tttggatacg tccaaataca gggtgagcag ataggagtag
     6481 tttatagttg cgacgattat tgcgtattgt taataccaat tcatggtcaa aaggttggtt
     6541 aactttttgg atacgtcctt tttcaatctg ttcttgtaat tcatttgtga ggtgatgtaa
     6601 aaaaaatcca tcaaaagaca tggcgtcccc cttattctaa aaattatagc ttatttatta
     6661 taccataggc tgagctaaaa atattttttt ataacgcaaa aggcttgcaa ttttcagaat
     6721 atagtatata attatagaaa aattcatgag aaaggatgac tagtaaatga ctaaaacatt
     6781 atttaccaat tggcaaaact ggcgtagata aagttgtgtt tatgggtgca tagatacgac
     6841 tttttggagt acttcaaaaa agtatctatg cgctggtcta gtctaacaat agagagcgca
     6901 aggtaagtta tttatcaggc gctttttctt ttgtgctaaa ctccagaaag tcaggatatt
     6961 attaaagtga ggagaaaata tgaaaaataa aggactgata gctacgctaa ttctattgac
     7021 catattagtg gttggggaat tattttataa caaatcagaa aaacatctta atttatcaga
     7081 aaagcaagtt gttaaaattg gtattttaca gtatgtaacc catgatgctt tggatgctat
     7141 tgaaaaggga gttgaagatg gtctagctca agaaggatat aaagggaaaa aagttaaatt
     7201 aaccgtttta aacgccgaag ctgatcaaag taaaattcaa gcgatgagta aacaattagt
     7261 taatcatcac aatgatattt tgattggaat tgcgacacca tcagcacaag gtttagcagc
     7321 ttcgactaag gatactccta taatcatggg agctgtttct gatcctttag gggccaagtt
     7381 agtaacgaat atgaagaaac caacaactaa tgttacagga ttatcaaacg ttgttccgac
     7441 caagcaaact gtgcaattaa tcaaggatat aactcccaat gttaaacgta taggaattct
     7501 ttacgctagt agcgaagata attctgtgtc acaagtaact gagtttacaa aatatgctca
     7561 aaaagcaggg ctagaagttc ttaaatattc tgtaccatct acaaatgaaa ttaaaacaag
     7621 tatgtcagtc atgaccaaaa aagtagacgc tgtctttgtt ccacaagata atactattgc
     7681 gagtgctttt agaacagtta tcgtagcagc aaaccaagct aatatacctg tgtattccag
     7741 tgttgatacc atggttgaac aaggaagtat agcatcagta gcgcagagtc aatatggctt
     7801 agggttagaa actgcaaaac aggccattaa agtcctaaga gggaagccgg ttaaagatgt
     7861 accagtcaaa gttattgata cagggaagcc aagcctcaat ttgaaagcag ccaaacactt
     7921 aggaattaaa attcctaaga agattatgaa acaggcagaa attaccgtta aggttgacga
     7981 ttagaaagag ggttttatga ttatttcatc agtttcacaa ggtttattat gggggatatt
     8041 aggattaggg atatacttaa cctttagaat cttaaaattt ccagacatga caacagaggg
     8101 ctccttccct ctagggggag cggtttgtgt taccttaatg aatcaagggg ttaatcctat
     8161 tttggcgacg attctaggca tgctatcagg gatgttagcg ggatttgtta ctggtttact
     8221 ttatactaaa ggaaagatac cgacgatttt agcagggatt ttagtaatga cctcatgcca
     8281 ctctattatg ctaatggtta tgaaaagagc aaatctaggt ttaaatgaga ttcaaacatt
     8341 gaaagatttt cttccgtttt caaatgattt aaatcttctc gtattaggtt tgatagctat
     8401 tctcttggcg atttctgtct taatttattt tctctataca cgcttggggc aagcttatat
     8461 tgcaacagga gacaaccctg atatggcaaa gagcttcggc attgataccg acaaaatgga
     8521 aatgcttggc ttaattgtat caaatggctt aattgcttta tcaggagctt tagtaagtca
     8581 acaagatgga tatgctgatg tatcaaaagg tataggtgtt attgttattg ggttagctag
     8641 tattattatt ggagaggtac tttactctac tggtttaact ctctttgaac gtttaattgc
     8701 tatagtggtg ggttctattc tttatcaatt tttaataacg gcagtaatcg ccttaggatt
     8761 taatacgaat tacctaaaat tatttagtgc aatagtgctt ggaatttgtt taatggttcc
     8821 tgtcctaaaa acgaagattt tgaaaggagt acgcttataa tgatgatgaa aatcatagag
     8881 ttaaaagaag caaccgtaca agtcagtaat ggtttagcag aaatgaaaac gatattagat
     8941 catgttaatt tgagtattta tgaacatgac ttcattacaa tattgggtgg aaatggagct
     9001 ggaaaatcaa cgctttttaa tgtaattgca ggtaccttga tgttaagcag tggaaatatt
     9061 tacatcatgg gacaagatgt tactaattta tcagcagaaa aacgagctaa atatttatca
     9121 cgggtatttc aagatccgaa aatgggaaca gcacctagga tgacagttgc tgaaaattta
     9181 ttagttgcta aatttcgagg tgaaaagaga ccgttagttc ctagaaagat tactaactat
     9241 accgaagaat ttcaaaaatt gattgctaga actggtaatg gacttgatcg ccatttagag
     9301 acacctacag gtttattatc aggtggacaa agacaagcgc ttagtttatt aatggcaact
     9361 ttgaaaaaac caaatctgtt attattggac gaacacacag cagcgcttga tcctagaaca
     9421 agtgtttcgc ttatgggctt gactgatgag tttatcaaac aagattcgtt gactgcctta
     9481 atgattacac atcatatgga agatgctctc aaatatggaa atcgtgtatt agtaatgaaa
     9541 gatggcaaga ttgtcagaga tttaaatcag gcacaaaaaa acaagatggc tatagctgac
     9601 tattatcaat tatttgatta aaaagaagtg aaatggaagt gtttcacttc tttttctata
     9661 cactttgcaa gaaattattt tcaaaatatg atatactaat aatgctgtaa atcgacttgt
     9721 agagacaagg accgtaagta caaatggaat gaaaataagg acacactctt taattcatat
     9781 tcctattatt gattaataag gtttttcttc aaacccaaga aaagttaccg ataaatcaat
     9841 tactaatgat aataattgga caatttcttg tctgctttat aaaaaagaat taacaaaatt
     9901 taaggagaaa actcattatg agcgacataa aaatcattgc tcttggaggt gttcgtgaaa
     9961 acggaaaaaa cctatatgta gtagaagtca atgattctat ctttgttttg gatgctggac
    10021 tcaaatatcc tgaaaatgaa caactgggtg ttgatgttgt tattccaaat cttgattact
    10081 taattgaaaa taaaaaacgt gttcagggga ttttcttaac ccatggacat gcggatgcta
    10141 ttggtgcatt gccttatatt atcgccgaag tcaaagcacc tgtgtttggt tcacctctca
    10201 caattgaatt agctaagtta ttcgttaaaa atagtacagc agtcaagaaa ttcaataatt
    10261 tccatgtgat tgatagtgaa acggaaattg aatttcaaga tgcggtgatt tcgttcttta
    10321 aaacgactca ttcaattcca gagtcaatgg gaattgttat tgggacaaaa gagggaaata
    10381 tcgtttacac aggtgacttt aaatttgatc aagctgcaag gaaatattac caaacagatc
    10441 tggcgcgttt agctgaaatt ggtcgtgatg gtgttttagc acttttatca gattctgcaa
    10501 atgctacaag caatgagcaa gttgctagcg aatatgaggt cggtgatgaa attaagagcg
    10561 ttattgaaga cgcagaaggt agagtcatcg tagcggcagt tgcaagtaat ctcattcgta
    10621 ttcaacaagt ctttgatgca gcggcagaaa acggtcgtcg agtagtttta acgggttttg
    10681 atattgaaaa tattgttcgt acggccattc gtatgaaacg cattcatatt gctgatgaaa
    10741 atatgattat caaacctaaa gatatgacac gttacgaaga taacgaatta ttaatccttg
    10801 aaactggacg tatgggagag ccgataaatg gtctccaaaa aatggcgatc ggacgtcatc
    10861 gttatgttca aattaaagat ggtgatttag tatttatcgt aacaacacca agtattgcaa
    10921 aagaggcagt tgtagcgcgt gttgaaaatc tgatttataa agctggtggc tcagttaaat
    10981 tgataacaca gaatttgcgc gtatcaggtc atgcaaatgg tcgcgaatta caattattaa
    11041 tgaatctatt gaaacctaaa taccttttcc caattcaagg agagtatagg gatttgtcag
    11101 cacatgcggg cttagctcaa gaggtaggaa tgtcagcaga tgatatttat attgtgaagc
    11161 gtggtgacat catggttctt gaaaaagatg gcttcttcca tagtggatct gttcctgctg
    11221 gagatgttat gattgatgga aatgctattg gtgacgttgg taatattgta cttcgtgacc
    11281 gcaaggtatt atctgaagat ggaattttta ttgttgttat aactgtcagc aaaaaagaaa
    11341 agaaaattat ttctaaagct cgtgttaata cgcgtggttt tgtgtatgtg aaaaaaagca
    11401 gagatatttt gcgtgagagc gcagaattag ttaatacaac ggtagaagat tacttaagta
    11461 aagatacatt tgactggggt gaacttaaag ggaaagtgcg tgatgaggtc agcaagttct
    11521 tatttgacca aacgaaacgt cgcccagcaa tccttcctgt ggttatggaa gttcgttgat
    11581 agtgttaaga ggttaccaaa ttttggtaac ctttcttgct agaatggagg tttttatggc
    11641 attttttaat attgaatatc actctaaggt actaggaacg gaacgtcaag tcaatgttat
    11701 ttacccagat gcttttgaga tgtctgatga taaaattgat gattgtgaca ttcctgtttt
    11761 gtatcttctc catggaatgg ggggaaatga gaattcttgg caaaaacgca caaatataga
    11821 gcgtctttta cgtcatacta atcttatcgt cgtgatgcca tcaacagact tagcatggta
    11881 taccaacaca aagtatgggt tggattattt tgatgcaata gctattgagt taccaaaagt
    11941 tttgaaacgt ttctttccta atatgtctga taagagagag aaaaatttca ttgccggatt
    12001 atctatgggg ggatatggag catataaaat agctctactg actaatcgtt tttctcatgc
    12061 tgcctctttg tcaggtgcac tttcttttga ctttgattta ttattcaata atggcaacga
    12121 taacactaac tattggtcgg gaatttttgg tgatttgaat aacactgata acatagagcg
    12181 tcattcacta aggcgttacg ttgagtcttt tgatatgaag actaaatttt atgcatggtg
    12241 tggttatgaa gattttcttt ttgaagctaa tgaagtcgca attgatgaac ttcgtcaatt
    12301 aggtttaact attgactatt ccaatgatca tggtaaacat gaatggtatt actggaatca
    12361 acaattagaa aaagtcttag aatggctccc tgttgattat gtcaaagaag agcgcctttc
    12421 ttagtattag ctaaatcgtt gaaatccaag taaagtcttg ttatactaaa tcattaagtt
    12481 ttgggaaatt agacacagaa ggtgagataa aaaatgagaa cactatttag aatgatattt
    12541 gctattccaa agtttatctt tagattgatt tggaatatca tttggggaat attcaagaca
    12601 gttcttgtta ttgcgattat tttatttggc ttgtattact atgcgaatca cagtcaatca
    12661 gaatttgcta atcaacttag tgacattatt cagacaggaa aaacattttt aaattttgct
    12721 gacactaatc agctcaaaaa ttcctttgct aatctagcta cggataatgt tcatcattca
    12781 gaaaatacta gatgggataa aaatcaggca actatctata tagcaactag agattctgag
    12841 ttggcctctg cttacaagca agcaatctcc aattggaatg caacaggtgc ttttgtgtta
    12901 caaacaacag ataatcctaa tgctgatatt attgctaagg attactcaga tgctaaaaca
    12961 caagctgcag gtgtagctga aaccgagaga aatgctttaa ctaatcgtat ttcaaaggta
    13021 acagttaaat taaacacatt ttaccttagg aataaacagt ttggttatga ccatactagg
    13081 attgttaata cggccgaaca tgaattagga catgctttag gtctaggaca taatgatcaa
    13141 caacattccg ttatgcagtc aaaaggttcg cattatggca ttcaagaagt tgatattcaa
    13201 acgctaaaaa acttatatgc ataaaaagat gagctaataa actcatcttt tttgtttgaa
    13261 cacttatttt aggtgatata agtgcgttag caatagatta ttaaaaataa agaatttgac
    13321 tatcactact aactaaaatg cataaagaac aaagaaaatg ttttcaaaaa tcacttaaaa
    13381 aatggtataa tgtgtcttga gatagtgttt tgaatagact tatcaaaaat attagataac
    13441 attaacttaa aaggggaaaa tatggacgaa ctaaaaaaac ttgctggagt gaccgctgca
    13501 aaatacgtta aaaacggtat gattgtgggt ttagggacag gttctacagc atattttttt
    13561 gttgaagaaa ttggacgccg tgtcaaagaa gagggattgc aagtcatagg cgttacaacg
    13621 tcaaaccgta cgactgagca ggcaagagga ttaggtattc cgttgaaatc agcagatgat
    13681 attgatgtca ttgatgtcac tgtggatgga gcagatgaag tagaccctga ttttaatggt
    13741 atcaaggggg gcggtggtgc gctgctcatg gaaaaaatag tagctactcc aactaaagaa
    13801 tatatctggg ttgtcgatga gagcaaactt gttgaaacgt taggtgcatt taaattgcct
    13861 gttgaggttg ttagatatgg ttctgagcgt ctttttcggg tttttaaatc aaaaggttat
    13921 tgtccttctt tccgtgaaac agaaggtgac cgctttatca ctgatatggg gaactacatc
    13981 atcgacctag atttgaaaaa aatagaggat ccaaagcagt tagcgaatga acttgaccat
    14041 acagttggtg ttgttgaaca tggtttattt aatggtatgg ttaataaagt tatagtagca
    14101 agcaaaaatg gtcttgacat acttgaaaaa taattattta aataatcggt tttaggagct
    14161 aaccgtaacc tccttagaaa gaagatataa tgtcacaatt tgatcgcatt cacttagttg
    14221 tcttagattc agtgggtatt ggagcggcac cagatgccaa tgattttgtc aatgcaggag
    14281 ttcctgatgg tgcttcagac acgcttggcc acatttcaaa aacagttgga cttgcggtgc
    14341 caaacatggc taaaatcgga cttggtaata ttcctcgtcc ccaagcgctt aagaccgtcc
    14401 ctgcagaaga aaatccaagt ggctacgcga cgaagttaca agaagtatca cttggtaagg
    14461 atacaatgac agggcattgg gagatcatgg gcttaaatat tacagagcca tttgatactt
    14521 tctggaatgg ttttccagaa gatattatca caaagattga ggatttttca ggacgtaaag
    14581 ttattcgtga agcgaataaa ccttattcag ggacagctgt tattgatgac ttcggaccac
    14641 gccaaatgga aactggtgag ctcatcatct atacctcagc tgatccagtc ctacaaattg
    14701 cagctcatga ggatatcatc cctctagaag aactctaccg tatttgtgaa tatgctcgta
    14761 gtatcacaat ggagcgtcca gctctccttg gtcgtattat cgctcgtcca tatgtgggtg
    14821 a
//
LOCUS       DATRNX010000044        14391 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRNX010000044 DATRNX010000000
VERSION     DATRNX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            1..792
                     /locus_tag="VBC81_001098"
     CDS             1..792
                     /locus_tag="VBC81_001098"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6912147.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            888..1322
                     /locus_tag="VBC81_001099"
     CDS             888..1322
                     /locus_tag="VBC81_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6912148.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            1322..2293
                     /locus_tag="VBC81_001100"
     CDS             1322..2293
                     /locus_tag="VBC81_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6912149.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            2351..2575
                     /locus_tag="VBC81_001101"
     CDS             2351..2575
                     /locus_tag="VBC81_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6912150.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            2730..3689
                     /gene="fabK"
                     /locus_tag="VBC81_001102"
     CDS             2730..3689
                     /gene="fabK"
                     /locus_tag="VBC81_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6912151.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            3709..4635
                     /gene="fabD"
                     /locus_tag="VBC81_001103"
     CDS             3709..4635
                     /gene="fabD"
                     /locus_tag="VBC81_001103"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6912152.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYCLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            4644..5378
                     /gene="fabG"
                     /locus_tag="VBC81_001104"
     CDS             4644..5378
                     /gene="fabG"
                     /locus_tag="VBC81_001104"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6912153.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            5394..6626
                     /gene="fabF"
                     /locus_tag="VBC81_001105"
     CDS             5394..6626
                     /gene="fabF"
                     /locus_tag="VBC81_001105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6912154.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDVDRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            6592..7128
                     /locus_tag="VBC81_001106"
     CDS             6592..7128
                     /locus_tag="VBC81_001106"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6912155.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            7125..7547
                     /gene="fabZ"
                     /locus_tag="VBC81_001107"
     CDS             7125..7547
                     /gene="fabZ"
                     /locus_tag="VBC81_001107"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6912156.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            7585..8955
                     /gene="accC"
                     /locus_tag="VBC81_001108"
     CDS             7585..8955
                     /gene="accC"
                     /locus_tag="VBC81_001108"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6912157.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            8964..9839
                     /locus_tag="VBC81_001109"
     CDS             8964..9839
                     /locus_tag="VBC81_001109"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6912158.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFIDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            9832..10605
                     /locus_tag="VBC81_001110"
     CDS             9832..10605
                     /locus_tag="VBC81_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6912159.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            11073..11705
                     /locus_tag="VBC81_001111"
     CDS             11073..11705
                     /locus_tag="VBC81_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6912160.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(11751..13028)
                     /gene="serS"
                     /locus_tag="VBC81_001112"
     CDS             complement(11751..13028)
                     /gene="serS"
                     /locus_tag="VBC81_001112"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6912161.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            13327..14388
                     /locus_tag="VBC81_001113"
     CDS             13327..14388
                     /locus_tag="VBC81_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO6912162.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
BASE COUNT         4564 a         2200 c         3087 g         4540 t
ORIGIN      
        1 ttgaagttcg aaaatataat atatggtatt gatggcagcg tggccactat tatgcttaat
       61 cgaccagata tttcaaatgg ttttaacatc ccaatgtgtc aagaaattat tgatgcaata
      121 aggcttatgt ctgaaaataa agatgtcatg tttttagtga ttgaagcaca aggaccaatc
      181 ttttcaatcg gaggagattt aaaagtgatg aaagatgctg ttgagagcga tgatattagc
      241 tcattaacta aaattgctga gttagttaat cagatatcgt atgatctact acagctagag
      301 aaacctgttg ttatgtgtgt agatggggct gtagctggtg ccgcagctaa tattgcatta
      361 gcagctgatt ttgttatcgc aagcaagaag agtaaattta tccaggcttt tgtgggtgtc
      421 ggtttagctc cagatgcagg aggtttattg ttacttagta aatcaatcgg tataactcgt
      481 gcagtgcaac tagctttaac tggagaaagc ttatctgctg aaaaagctga agctttggga
      541 attgtttata aactttgtga atcagataaa ataggtaaaa ttaaggatca actattaaag
      601 cgtttaagta gacattctat aaactcatat caagccataa aatcattagc ttgggaagct
      661 gcttttaaag attgggaaca atataaaaag cttgaattac agttacaaga atcattagcc
      721 tttaagcaag attttaaaga gggtgttcgg gcacacgctg atagaaggcg tcctaatttt
      781 ttaggcaaat agtttgaaaa tcaaattatt taagattaaa gtcttgcaaa taagtttgaa
      841 ctttgatata atttgactat caaagtttaa tgtgaggtgg tgatatcttg gaatacgatc
      901 agattaatag ttatttagtt gacattttta atagaatcat gattattgag gaaatgagtt
      961 taaagactag ccaatttagc gatgtatctt taaaagaaat gcatactatt gaaattattg
     1021 ggaaacattc tgaggtaact cctagcgatg tcgctcgaga attaatgtta actttgggaa
     1081 ctgtaacaac cagccttaat aagttagaaa aaaaaggcta cattgagcgt aaacggtcaa
     1141 gtattgatcg tcgagttgtt cacttgagtc ttactaagag aggtaggtta ttagatcgat
     1201 tgcatagtaa atttcataaa tctatggtat ctcatattat tgaagaccta ggcgaagaag
     1261 acataaaaat gttgacgagc gctttaggga atcttcataa atttttggag gacttagtct
     1321 agtggttttt gctaaaatta gtcaacttgc acattatgca ccttctcaaa tcattaaaaa
     1381 tgaggattta tcattaatca tggataccag tgatgactgg atttcgtcac gtactggtat
     1441 aaaacaacgt catattagta aaaatgaaac aacagctgat ttggcaaata aggtcgcaga
     1501 acaacttatt gaaaaatccg gttattccgc tagtcaaatt gactttatta ttgttgcaac
     1561 gatgacacca gactctatga tgccttctac tgccgcaaga gtgcaagctc atattggagc
     1621 gagtaatgct tttgcctttg acttgagtgc agcatgtagt ggttttgttt ttgccttgtc
     1681 taccgccgaa aaattgatat catcaggtag ctatcaaaaa ggtttagtaa taggagcaga
     1741 aactgtttct aaagtattag actggactga tagagggaca gcagttttat ttggagatgg
     1801 agctggaggt gtcttgctag aagcttctaa ggaaaaacat tttcttgcag agtctctcaa
     1861 tacagatggt agtcgtcagg gacttcaatc tagtcaagtc gggttaaatt cacctttctc
     1921 agatgaagta cttgatgata aatttttgaa aatggatggt agagcaatct ttgattttgc
     1981 tatcaaagaa gtttcaaaaa gtatcaatca tcttatagaa acatcccatt tggaaaaaga
     2041 agatattgat tatcttttct tacaccaagc gaatcgaagg attttagata agatgtctcg
     2101 aaaaattgat atagcacgtg ataaatttcc agagaatatg atggattatg gtaatactag
     2161 tgcagctagt atccctattt tactttcaga atcttatgag aacggtttgt tgaaactaga
     2221 tggtaaccaa acaatcctct tatcgggttt cggtggaggt ttgacatggg gaagtctaat
     2281 tgttaaaatt tagttatata tagtgcatag cacatttata aaagattatt taaaatttaa
     2341 ggagctaaaa atggcagtat ttgaaaaagt acaagaaatt attgttgaag aacttggtaa
     2401 agatgcagaa gaagtaacgt tgaacactac ttttgacgat cttgatgctg attcgcttga
     2461 tgttttccaa gtgatttcag aaattgaaga tgcatttgat atccaaattg agacagaaga
     2521 aggactaaat actgttggtg atttagttgc ttatgttgaa gaaaaagtaa aataattaag
     2581 tcgtaagaaa aaaagaagca agtgataata aaagatattc ttaccaaatc acttattaga
     2641 gtgagtattc aatcaatcgt tgatcggtac tcactcttct tcttatcaat atagtttgat
     2701 tgtcaaatta ttaattttag gaaagaaaaa tgaaaacacg tataacagaa ttactaaata
     2761 taaaatatcc aatttttcaa gggggtatgg cgtgggtcgc tgatggagat cttgcaggag
     2821 ctgtttctaa ggcaggtggt ctaggtatta ttggtggagg aaatgcccct aaagaggttg
     2881 tcaaagcaaa tattgataaa ataaaatcta tgactgataa accctttggt gtgaatatta
     2941 tgcttttatc accttttgta gatgatattg ttgatttggt tattgaagaa ggtgtaaaag
     3001 ttgtaacgac tggtgcaggt aatcctggta agtacatgga acgctttcat gaagcaggta
     3061 taacagtaat ccctgttgtt cctagtgttg cattagcaaa acgtatggag aaattagggg
     3121 ctgatgctat cattacagaa ggaatggaag cgggtggtca catcgggaag ctgaccacga
     3181 tgacattagt gcgacaggtt gttgatgctg ttacaattcc agtcattgcc gcaggtggta
     3241 tagcagatgg gcgtggggct gctgcgggtt tcatgttagg agctgatgct gttcaggtag
     3301 gaactcgttt tgttgttgct aaagaatcta atgctcaccc taattacaaa gcaaaaattt
     3361 taaaagcaaa agatatcgac actgctgttt ctgctcaggt agtaggtcat ccagttcgtg
     3421 ccttaaaaaa taaactagta acaacctata gccaagcaga aaaagattat ttagctggcc
     3481 gtatttcaat caatgagatt gaagaattag gagcgggagc tcttcgcaat gcagttgttg
     3541 atggggatgt tattaatggt tcagttatgg caggacaaat tgcaggatta attaaatcag
     3601 aagagacttg tcaagagatt cttgaagata tttattcagg agcgcgtcaa gttattcttt
     3661 ctgaggcaag ccgctggtca gatttataaa tacaaataag gagacactat gaataaagtt
     3721 gcatttctat ttgctggtca aggtgctcaa aagttaggaa tggctagaga tttatatgaa
     3781 actttcccga ttgttaaaga aacttttgat aaggcaagtc atgttttagg atatgatttg
     3841 agagaattga ttgataaaga cttagacaaa ttaaatcaga cgaaatatac gcaaccagct
     3901 attttaacga cttcgactgc tatttattgt ttaattctta aagaaattgg acttagacca
     3961 gatatggttg caggactttc tttgggagaa tatagtgctt tagttgcttc aggtgctata
     4021 agatttgaag atgctgtagt acttgtttct agacgaggcc aactaatgga agcagcagca
     4081 cctgctggta gtggcaaaat ggtagcggtt ttaaatgctg atcgtcaaat aattgaagat
     4141 gcttgtaaga aggcttctca gtttggtatt gttagtcctg caaattataa tactccaaag
     4201 caaattgtta tagggggaga gtcaatcgct gtaaatgcag ctgtagaaga actaaaacaa
     4261 caaggagtaa aacgtttaat tcctctgaat gtttcaggtc cttttcacac tgctttattg
     4321 aaacctgcaa gtcagaaatt gagcgatgtt ttagataaag ttcatttttc agtttctgaa
     4381 atccctgtaa ttggaaatac agaggcgcag ataatgaaaa aagacgatat caaatcttta
     4441 ttggcacgcc aagttatgga acctgtacgc tttgatgagt ctattgaaac catgaaaaaa
     4501 atgggaatga cacaggttgt tgaaattggt ccagggaagg tactctcagg tttcttgaaa
     4561 aaaatagata gctcattatc agtatattct gttgaagata aaattggatt taataatctt
     4621 aaggaattaa actaggggaa aatatgcaat taaaagataa gaacattttt attacgggtt
     4681 caagtagagg aatcggcctt gcaattgccc atcaatttgc acaattaggt gctaatattg
     4741 ttttaaatgg acgttctgaa atatcagaag acttaatagc tgaatttgct gattatggtg
     4801 taaaagttat tgctatttca ggagatgttt cttcttttga agatgctaat cgcatgatta
     4861 aagaagctat tgcaagtcta ggtagcgtgg atgtgcttgt taacaacgct ggtattacta
     4921 atgataaatt gatgcttaaa atgactgttg aagattttga gagcgttctt aaaattaatt
     4981 taacaggtgc atttaatatg acacaatcag tcttgaaacc gatgacaaaa gcgcgtcaag
     5041 gtgctattat taatatctct agcgtcgttg gattaactgg aaatgtaggg caagctaatt
     5101 acgcagcctc taaagcaggt ttaattggct ttacaaaatc agttgccaga gaagtagcag
     5161 cacgaggcat tcgtgtcaat gccattgctc caggctttat cgaatctgat atgacagatg
     5221 ttatcccaga aaaaatgcaa gaagctattt tagctcaaat tccaatgaaa cgtattggaa
     5281 aaggtaaaga agtagcgcag gtagcgtctt tcttagcaga acaagaatac cttacaggac
     5341 aagttattgc cattgatggc ggtatgacga tgcaataata aagaggaaat aatatgacat
     5401 tacagcgagt agtagtaaca ggttacggtg taacctcacc tattggaaac acaccagaag
     5461 aattttggaa tagccttaaa gaaggaaatg ttggtattgg acctatcact aaatttgatt
     5521 caagtgattt catggtcaaa aatgctgctg aaattcatga ttttccattt gataaatatt
     5581 ttgtcaaaaa agatctgaac agatttgata tgtattccct ttatgcctta tatgcttcaa
     5641 gtgaagctat tcaacatgct aatttaaatt tagatgaaat tgatgtagat cgatttggtg
     5701 tcatcgttgc atcaggtatt ggagggattc aagagattga agaacaagtg attcgtcttc
     5761 atgaaaaagg gccaaaacgc gtaaaaccaa tgacgcttcc aaaagctcta ccaaatatgg
     5821 cagctggaaa tgttgcgatg aggttaggcg ctcatggtgt atgtaaatca attaatacag
     5881 cttgtgcttc ttctaatgat gctattggag atgctttccg taatataaaa tttggtattc
     5941 aagatattat ggtagtcggt ggtgcagaag ctgctattac aaaatttgcc attgctggat
     6001 tccaaagtct tacagcatta tcaacaactg aagatccatc aagagcatct attccatttg
     6061 ataaagatcg caatggattt atcatgggag aaggttctgg aatgttggtc ttagaaagtt
     6121 tagaacacgc tgaaaaacga ggagctacta ttcttgcaga agttgttggt tatggtaaca
     6181 cttgtgacgc atatcatatg acttcaccac atccagaagg tttaggagcc actaaagcta
     6241 ttcaacttgc actggctgaa gctaatatta aacctgaaga agttaactat gtaaatgctc
     6301 atggaacttc aacacctgct aacgaaaaag gagagagtca agctgttgtt gctgcacttg
     6361 ggacagacgt tccagtttct tcgactaaat cttttactgg tcatcttttg ggtgccgcag
     6421 gagctgttga ggcaattgca actattgaag cgatacgtca tagttatgtt ccaatgactg
     6481 ctggaacaac agaattatca gaagatatta cagcgaatgt tatttttggt caagggcaag
     6541 acgctgatat tcgttatgcc atttcaaata catttggttt tggaggacac aatgcagttc
     6601 tggccttcaa acgctgggag gattaaattg gatattcaag agattaagga tttaatgact
     6661 caatttgatg agtcaagtct gagagaattt tcattcaaaa caagtgacgg tgagctgtct
     6721 tttagtaaaa atgagggaaa agctccactt gtaccaacta tgtctcctat gagtcatcaa
     6781 cctgaagcga ctccaactat agcaactcct gtctctaatg aagcaggtga gcaaacgaaa
     6841 caagctactg aagtagtttc agaaatccca gagtcgactg tgactgttgc agaaggtgac
     6901 gttgtcgaaa gtcctttagt tggggttgct tatttagctt ctggaccaga taaacctaac
     6961 tttgtttcag taggtgatag cgtgaagaag ggacaaactt tgatgattat tgaggcaatg
     7021 aaggtgatga atgaagtgcc tgcccctcat gatggtgtag ttactgaaat tcttgtagcc
     7081 aatgaagaag ttattgaatt tggtaaagga ttggtacgaa tcaaatgatt gatataaaag
     7141 aaattcgtga ggctttacct caccgctatc caatgttatt agtggatcgt gttttagagg
     7201 tatcagaaga tgagatagtc gctatcaaaa atgttagtat caatgagcct tttttcaatg
     7261 gtcattttcc agaatatcct gtaatgccag gcgttttaat tatggaagct ttggctcaaa
     7321 ctgcaggtgt tttagaatta tctaaagaag aaaacaaagg aaagcttgtt ttttatgcag
     7381 gtatggataa agtgaaattt aaaaagcaag ttgttccagg agatcaatta gttatgactg
     7441 ctaagtttgt caaacgtaga ggaactatag ccgttgttga agcaatagca gaagttgatg
     7501 ggaaattagc tgccagtgga acgttaacat ttgcaatagg taattgaaga tagtattgaa
     7561 tttagaggga gagaagcatc aaaaatgttt aagaaaatat tgattgctaa tcgcggagaa
     7621 attgcagtac gtatcattcg tgcagcccgt gaaatgggaa tttcgacagt tgctatctat
     7681 tcggaggctg acaaggaatc attgcacaca attttagcag atgaagctat ttgtgttgga
     7741 ccagccaaat cagcagaatc ttatttaaac gttaatgcta tattatctgc tgctattgtg
     7801 actggagcgg aagctgttca tccaggtttt gggtttttga gtgaaaattc taaatttgct
     7861 actatgtgtg aagagatgaa cttaaaattc attggtccat ctggagaagt tatggataaa
     7921 atgggtgata aaatcaatgc tcgtgcagag atgataaaag ctgatgttcc ggttatccca
     7981 ggatcagatg gtcaagttac aagtgtagaa gaagctgtat caattgctga ggaaatcggc
     8041 tatccattga tgttaaaagc ctcagccggt ggcggtggaa aaggaattag gaaggtcaaa
     8101 tcagcagatg aattgaagcc tgcttttgag tctgcttcac aggaggcttt ggctgcgttc
     8161 ggtaatggtg ctatgtatat tgaaaaagtt atatacccag ctagacatat tgaagtacaa
     8221 atacttggag atagctttgg taagatagtt cacctaggag aacgtgattg ttctctgcag
     8281 cgtaacaatc agaaggtatt ggaagaaagt ccgtctgtgg caataggtaa cactctgcgt
     8341 caacaaattg gtgaagcagc tgttcgtgca gctgaggctg tatcttatga aaatgcagga
     8401 acaattgagt ttttattaga tgagaatagc ggacagttct atttcatgga aatgaatact
     8461 cgtgttcaag tagaacaccc tgttacagaa tttgtaacgg gtgttgacat cgttaaagag
     8521 cagattcgta tcgctgctgg aattccttta tcagtttctc aaaatgatat taagttaact
     8581 ggtcatgcga ttgaatgtcg tattaacgca gagaatccac aatttaattt tgcgccatgt
     8641 ccaggtacca ttaatgggtt acatctacct gctggcggta tgggcttacg tgtcgattca
     8701 gcggtttaca caggctacac tattcctcct tattatgata gtatgattgc gaaagtaatt
     8761 gttcacggag aaaatcgttt tgatgcactt atgaaaatgc aacgtgcctt atatgagctt
     8821 gaaattgatg gtattataac aaatacagaa tttcaaatgg atctcatttc tgataaaaaa
     8881 gtcttggctg gagattatga tacttcattc ctgatggaag actttttacc aaggtattat
     8941 ggtaacaatc aataggaggg aagatggctt tatttagtaa aaaggataaa tatatccgta
     9001 ttagtccaaa caaagcatta gggtcttctg ataaacgtag tcttcctgag gttcctgatg
     9061 aactctttgc taagtgtcct tcttgtaaac atatgattta tcagaaagat ttaggattag
     9121 caaagatttg tccagcttgt tcttacaatt ttagaatttc agcgcaagaa cgtctgttgc
     9181 taacagttga cgaagatagt tttgaggaat tatttacagg gattgaaact aaggatcctt
     9241 tgaattttcc aaactaccgt gaaaaactgg ctgctacacg tcaaaagact aacttagatg
     9301 aagcggtggt gacaggttta gctaaaataa aaggacaaac tacggcctta gctattatgg
     9361 attctcattt tataatggcg tctatgggaa cggttgtagg cgaaaagttg acaagattat
     9421 ttgagctagc aactgaaaag aaattaccta ttgttatttt tacagcgtca gggggcgcca
     9481 gaatgcaaga aggtattatg agtttgatgc aaatggcaaa agtatcggca gcagttaaac
     9541 gccattctaa ccagggattg ttttatttaa caattttaac agacccaaca accggcggcg
     9601 ttactgcgag ttttgctatg gaaggtgata ttattctggc tgagccgcaa gcgttggttg
     9661 gttttgctgg tcgtcgtgtt atcgaaacaa cggtgagaga agatttacct gaaggctttc
     9721 aaaaggcaga gtttttgtta gaacatggtt ttattgatgc tattataaat cgaacagaat
     9781 tacgcgactg cattgctcaa ctaattgctt tccatggaaa tgaggttggt catgaataat
     9841 gatgtgacaa gaattttaaa agatgcgcgt gatcaaggga ggctaactgc tcttgactac
     9901 gctgaattga tttttgataa ttttatggaa cttcatggtg atcgccagtt cgcagatgac
     9961 aaatcaatta taggaggttt ggggtacttg gcagggcgtc ctgtgacgat tgtcggtatt
    10021 caaaaaggta agaaccttca agataattta gaccgtcatt ttggtcaacc acatcctgaa
    10081 ggttatcgta aggctttacg tttaatgaaa caagctgaga agtttggaag acctgttata
    10141 accttcatca atactgctgg agcatatcct ggggtaggtg ctgaggagcg aggtcagggt
    10201 gaagcaattg ctagaaacct cttagaaatg agtgatttaa aagtccctat tatagctatt
    10261 attattggtg aagggggttc tggaggagca ttagctttag ctgttgccga taaggtttgg
    10321 atgttggaac atactgttta ttctatttta agtcctgagg gctttgcctc tattttatgg
    10381 aaagatggta caagaacaac agaagcagca caattaatga agatgactgc tggagaactt
    10441 taccatatgg aagttgtaga caaagtaatc cctgaacatg gttatttttc aagtgagatt
    10501 gtcgatatga ttaagacaag tttaatttca gagttagaaa tgctctcaca attatctctt
    10561 gaggatttat tggagcaacg ttaccaacgt tttagaaaat attaatagaa aaaggctgag
    10621 ttatttactc agcctatatt tagtttttca aggtattatc atagttatag tttcatattt
    10681 tgctataatt gataaaaagg agatatgtca atgacactag cattagccaa tttttagatt
    10741 gtagtctaat ccaaaatttt cagaaagagt catttcgaac actttattct aagtaccatt
    10801 atttaggctc tccttaactt gagactctat ctgttattga agagaaattt tctagaaaaa
    10861 atagctattg ttagatttta gaaggctatg aaaagattgg tttttaaaga gttctattaa
    10921 gtgatgaaga aagtaaaact tggttagtta taattgcgat tagactttgt ttctagcaac
    10981 gagacttcgc tatacatact ttaaaagaac ttgaaagtat gtatagatat ataatcgtga
    11041 aaacttaact attgtttaca tggagaaaaa gaatgtatca agaattaaat gattatttag
    11101 aattccaatc aatgaagtat attatgcctg agaaggcagg ccctttaaag gacgatatgc
    11161 aaacttttaa ggcaaagggc caacttgcta ggaatagctt tacagagtta tcaagagcct
    11221 tagaacaacg tatggatggc tttaaaatgc aaagagtgag taattgggct aatcaagctc
    11281 aagtagggcg tccacatttt tgggtttatt atcgaaagga tacagaccaa ttagatgatg
    11341 tggctgttgc gctaagggtt tatggtgtaa aagatagttt tggggttagt cttgaggtaa
    11401 gctttgtaga gaggcaaaaa tctgataaga cactcgagaa gcaggctaga gtgttatcga
    11461 ttccgattgc ctctccattg tattttatgg tccaaaggca aggtgaaact catcgagagg
    11521 aaggcaatga ggaaaatcgt caacgtttaa tgcaagaaat taagtcaggg aaggtaagga
    11581 aagtattggt aaaatatgat gttcttttga ctgaaaacca gagcttagaa aatattctgc
    11641 aacggttgtt agaaggattt gagaaagtgc taccttacta ccaagtttgt caaactatta
    11701 gctaagttca tggaaaaaat gagttggtat accaactcat tttagagtgc ctaatttggt
    11761 ttaataatat cgatattacc catatatgga cgaagaactt ctgggatagt cacagaacca
    11821 tcttcatttt gataattttc gaggattgct gctacagtac gtccaactgc aagtcctgaa
    11881 ccatttaaag tatgaagcaa cctaactttg ccatcaactt cgtcacggta acgaatttga
    11941 gcacgacgtg cttggaaatc ttcagtatta gaacatgaag aaatttctcg ataagtattt
    12001 tgagcgggaa tccatacctc taagtcatac gttttagctg ctgagaatcc catatctcct
    12061 gtacacaagg taatgacacg gtatgggaga ttaagttttt gaagaatatt ttcagcattt
    12121 gctgtcattt tttctaattc ttggtacgat tcctcaggct tggcaaattt caccatttct
    12181 actttatgga attgatgaag gcgaattaat ccacgtgtgt cacgtcctgc agagcctgct
    12241 tctgaacgga atgatggact catggcagta aagtatatag gtaattcttt cccatcgata
    12301 atttcatcac gataatagtt tgttaaagga acttcagctg ttgggataag tacgaatggg
    12361 ctatctgcta attcgaaagt atcttccttg aactttggat attgtcccgt accgaacatt
    12421 gaatcgtggt taaccatata aggagggatc acttcagtgt acccttcctt agcgtgttca
    12481 tccagcatga agctgtagat agcacgctct aaacgagcac ctagaccttt gtagaagagg
    12541 aagcgagaac cagtaacttt tgcaccacgt tcccaatcga gaattccaag gctttcccct
    12601 aaatcccagt gagctttggc ctcaaaatca aattcacgag gagttcccca gcgacgaact
    12661 tcaacatttt catcctcgtc tgctccaagc ggcacatcat cagctggtgt attaggaagt
    12721 acggtcacca ttgattgtaa attagcatcc acatctgcca attccgcatc aattgctttg
    12781 atatcagcgg aaagtgtttg catagctgtg atttgctcgt ctgcattctc tttatttcgt
    12841 tttgcttgag caatggcagc agaagcaaca ttacgttgcg ctttcgcctc ttcagatttg
    12901 ataagtaatt cacggcgttt tatatcaagt tctttaaggg ttgttaatgt ttcttggtca
    12961 acaccacgcg tggccaattt tttagcgaca gcatcaaaat ctgtacgaat acgttttaag
    13021 tctaacatat aggaatctcc tttatactaa aaaaaacacc ctgtcatgct ggagcgcaaa
    13081 ggcgcggttc catccagctt cacagaagtg cacttgattg attgattaaa ttatttccca
    13141 taaatggaac acggtagaat ttcataaaac aatcttatct gctcgcaacg accgcagact
    13201 ctctgaaaag aactattata ctacttatcc gttaaggtta ttatatccaa aaataagtaa
    13261 aatgtaaacc ttaactttat aagcctgctc tttgttttag tgaaatagct atgctaaaat
    13321 atgattatgg aaaagtatac aagtattaaa caatcaggtc gtcattacgg cttagacctt
    13381 ttaaggatta tttccatgtt catgattgtt attacgcatg ttcttggaaa aggtggtctg
    13441 agatcttcgg ttgagggaca cgcagactcc tattttatag taacgtggat tatccaagtc
    13501 ttagtttatg gggccgttaa ctgttacgca ctaataagtg gttatgtggg aattaattcg
    13561 cgttatcgtt actcaaaatt actgtctatc tggacacagg tttttttcta cacctttaca
    13621 ataacagcac tttttgcaat cacaggtcat gaagtgacct tgcttaattg gagagatgct
    13681 tttttcccta ttgtcagtgg gcagtattgg tacattactg cttactttgg tctattggtt
    13741 tttatgcctg ttattaataa tggattaaat gccttgactg ataaacaatt aaaacaattg
    13801 gttttgttaa tgtttattat tttttcaatt ttaccagctg ttttaaacaa tcgtgttcca
    13861 gaattttcat tgagcaaggg ttttgaaatg acttggctac tgattttata tattattggt
    13921 gcctatttga aacgtattga tttaaatata tttaaaacat catacttact tatagtttat
    13981 ctattatcat tggttgctac ttatgtcatg aagtttagta ttggtgatat ttggtattgg
    14041 tatgtttcgc ctactttaac tttaggggcg gtaagtttgt ttatattatt tgctagagca
    14101 agcattaagc cctcgggatt cttaaagaaa ataattgttg tattagcccc ttcaaccttg
    14161 ggagtttatc tctgtcatct ccacccacta atagtaaaat attttgtaag agattttgca
    14221 gagacgtttg tttatgagtc catttatctt tacccatttt taattttagg agcagggatt
    14281 ttaatttatt tgttggcttt tattgtagaa cgtattagat tacgaatatt taaagcaatc
    14341 catttttcta actttttgag atggtttgat gacaagttcc catattaata t
//
LOCUS       DATRNX010000045        14001 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRNX010000045 DATRNX010000000
VERSION     DATRNX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            197..1069
                     /locus_tag="VBC81_001114"
     CDS             197..1069
                     /locus_tag="VBC81_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6912163.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(1096..1404)
                     /locus_tag="VBC81_001115"
     CDS             complement(1096..1404)
                     /locus_tag="VBC81_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6912164.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(1492..3183)
                     /locus_tag="VBC81_001116"
     CDS             complement(1492..3183)
                     /locus_tag="VBC81_001116"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6912165.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGSYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            3405..3842
                     /gene="argR"
                     /locus_tag="VBC81_001117"
     CDS             3405..3842
                     /gene="argR"
                     /locus_tag="VBC81_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6912166.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            3899..6475
                     /gene="mutS"
                     /locus_tag="VBC81_001118"
     CDS             3899..6475
                     /gene="mutS"
                     /locus_tag="VBC81_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6912167.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(6532..6735)
                     /locus_tag="VBC81_001119"
     CDS             complement(6532..6735)
                     /locus_tag="VBC81_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6912168.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            6961..8934
                     /gene="mutL"
                     /locus_tag="VBC81_001120"
     CDS             6961..8934
                     /gene="mutL"
                     /locus_tag="VBC81_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6912169.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            8966..10198
                     /locus_tag="VBC81_001121"
     CDS             8966..10198
                     /locus_tag="VBC81_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912170.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            10200..10790
                     /gene="ruvA"
                     /locus_tag="VBC81_001122"
     CDS             10200..10790
                     /gene="ruvA"
                     /locus_tag="VBC81_001122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6912171.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            10813..11364
                     /locus_tag="VBC81_001123"
     CDS             10813..11364
                     /locus_tag="VBC81_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6912172.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            11453..12712
                     /locus_tag="VBC81_001124"
     CDS             11453..12712
                     /locus_tag="VBC81_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6912173.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTSGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            12786..13925
                     /gene="recA"
                     /locus_tag="VBC81_001125"
     CDS             12786..13925
                     /gene="recA"
                     /locus_tag="VBC81_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6912174.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
BASE COUNT         4520 a         2198 c         2867 g         4416 t
ORIGIN      
        1 aatatttaat gctaaaagca gatccaaaag cctttgtttc cgttgcagaa aatgttcata
       61 ttattggacg attcgttgat gatgattaac tgtatatatg acttttggtc aaccgccact
      121 aaggaaacat tatgatgtta ttaagctaag ttgtttaaac aactttagct tttttgaata
      181 aggaaggatt aagcctatgt tgaaactgga tttaaaaaca aagataaaag aagctatttt
      241 aattgccttt ggtgtggctc tctatacttt cggctttgtc aaatttaata tggctaatca
      301 tttggctgaa ggaggtattt ccggtgtcac tttgattatc catgcacttt tcggagttaa
      361 tccagcactt tcatcacttc tccttaatat cccgttattt atattagggg ctaggatttt
      421 agggaaaaaa tccttattat taactatata tggtacagtg ttaatgtctt ttttcatgtg
      481 gttttggcaa caaatccctg taacagtccc tttgaaaaac gatatgatgt tagttgcagt
      541 agctgctgga attttagcag gaactggtag tggtttagta tttagatatg gtgcaactac
      601 aggtggtgca gatattattg gtcgtattgt agaggaaaaa tctggaataa aattagggca
      661 aaccttattg tttattgatg ctatagttct aacgtcttca cttgtttata ttaatttaca
      721 acagatgcta tatacgttag tagctagctt tgtatttagt caagtgttaa caaatgttga
      781 aaatgggggc tacactgttc gtggaatgat cattattacc aaagaatcag aatctgcagc
      841 agcaaccatt ttgcatgaaa ttaatagggg tgtaactttt ttacggggcc aaggtgccta
      901 ctcaggtaga gagcatgatg tcttgtacgt agctcttaac ccaagtgaag tgcgtgatgt
      961 gaaagaaatc atggctgatt tagacccaga tgcttttatt tctgttataa atgtagatga
     1021 agttattagc tcagatttta aaattcgacg aagaaattac gataaataaa aatacaggct
     1081 gtctggcctg tatttttatt ttggtcctcc acttaagaaa ctaccaagca taatagctga
     1141 gatagcagtt cccttgtaat ggctagcttt tgtttttacc aagcgactaa gatcaaatat
     1201 gcttttagga gcatcaaatt gatgtgttaa gacgtattga ttgataagtg agtcaatttt
     1261 tgtagctagg agtgagttca tcccctcttt ttcaagttga ctagcgagct cgaaaatagc
     1321 ttgacgaaaa taatcatctg cttgtattac ttcttcgcta taaactttac taagagcatc
     1381 taatatttct ttttctgatg gcatggcatt ctcctgtaac ttattgtgct attattataa
     1441 tataaaaaat taagactgtc ataaatttga cagtcttagt aatgtcattt cttacatttc
     1501 attcggagca tcaactccta ggaggcgaag agattctttt aatactgtag cagttgcata
     1561 gcaaagtgct agacgactac tgatttcagc atcttcatct aaaatacgtg tgtgggcgta
     1621 atatttattg aagcactgag ctaaattgat agcgaatttt gcaataatag agggctcaaa
     1681 attatctgct gcacgcacaa taatacgagg aaaatcttgg atgagtttga tgatttccca
     1741 gctttcaaca tcgttgagac tgtaattatc gctattagat gggttaaagt tagctttacg
     1801 taagattgat tgaatacgag catgtgcata ttgtacatat ggcccagttt caccttcaaa
     1861 tgataccatt gcttctaagt caaagtcata accatttgta cgatcagttt tcaaatcgta
     1921 aaatttaata gcacctacac caactgcttg agcaacttta tctttatctg ctaggttagg
     1981 gtttttagct tcaatctgag atgccgcacg attaatagct tctgcaacag taggttcaag
     2041 gagaattaca tttcctttac gagttgataa ttttgctcct ccttttgtta caaggccaaa
     2101 aggaacatga gtcatatcat cggaccaatc gtagtccatt tctttaagaa cagcttttag
     2161 ttgtttaaag tgtgcagact gttcgtttcc tacaacgtag attgattttg caaaatcgta
     2221 ggtacgttta cggtaaagtg cagcagctaa gtcacgtgta atgtagagag ttgctccatc
     2281 ggatttttta atgagggcag gatgttcaat accgtatttt tctaggttaa caacttgtgc
     2341 acctttagat tcgactagga ggtttttaga ttctagcagt tcaagcactt catccatttt
     2401 gtcgttgtag aatgcctcgc cgttatagct atcaaatgta acattcattt tatcgtaaag
     2461 acggttgaat tctaataatg actcgtcacg gaaccattgc cataattcag ttgcttcagg
     2521 atcgttagcc tcaagtttac gaaaccactc acgtgcctct tcatcaacgc tagggtctat
     2581 ctcggcttca gcattaatac ggacataaag ttttaaaagt tcatcaatgg gatgagcacg
     2641 gacagcctct tcattgcccc attttttata ggcaacgatt aacattccaa attgtttacc
     2701 ccagtcgcca aggtgattaa ttttaacagg atgatagcca attttatcaa aaatattagc
     2761 taaactatca ccaataactg ttgaacgaag gtgtccaatt gagaatggct tagcaatatt
     2821 tggactagac atatcaatgg caacatttcg tccttcacca atattttgag tagcataggc
     2881 agaaccatca gataagactt gttttaaaac ttgacttgag attgcggctt tatcaaggaa
     2941 aaagttaacg taagatccaa cagcttccac cttttcaaat tggtcgcttt taatttgttc
     3001 agcaatgtca ctagcaatta tttgaggtgc tttgcgtaga gttttagcca aagagaatgc
     3061 tgggaaagct aaatctccca tgctcgaatt ttttggggtt tctaataaag aaagaatggc
     3121 cgattgttcc atatatggaa caactttttg aatttcactc gcaattagat gttttgtatc
     3181 catggtatct cctattttaa ttagtaatat agtcacccta ttttaacata attatgaaaa
     3241 atagacgaga acttgttgat taaattcata atgcattctg taaagaacta gcctacttta
     3301 tagctttttt acagaatgat tacgatatca ctactaagtg aaaaagacaa ttttttttga
     3361 tataatttgg taagcataat tctaactaaa aaggattgaa taacatgaat aaaatagaac
     3421 gccaaaaacg tattaaacgt ttaatccaat cagggcagat tggcacacaa gaagaaatta
     3481 aattacattt aaaaaatgaa ggaatcgatg tcacgcaagc gactctatca agagacttgc
     3541 gtgagattgg tcttttaaaa ttgcgtagcc cagaaggtaa gctatattat agtttatcaa
     3601 cagctacttc aaatcgattt agcccagcct tacgctccta tattttgaaa gtatcgcgag
     3661 caagttttat gttagtttta aatactaatt taggggaggc tagcgtttta gcaaacttta
     3721 ttgatgagaa agggctgcca gagattttag gaacaatggc aggagcagac accttgttgg
     3781 tgatttgtca aaatgaagat attgccaaag tttttgaaaa agaactatca gtaggtttat
     3841 aaatataatg attgaggagc tgagcatggc agccagcccc gttagttagg agtgagaaat
     3901 ggccaagcca acgatatcac cgggaatgca acagtatctg gatataaaag agaattatcc
     3961 agatgctttt ttgcttttta gaatgggtga tttttatgaa ttattttatg atgatgcggt
     4021 aaaagcagca caaatcctgg aaattagctt gactagtcga aataagaacg cagaaaagcc
     4081 aatcccaatg gcaggagttc cctatcactc agctcaacag tatattgacg ttttagttga
     4141 attaggttac aaagtagcca ttgctgagca gatggaagat cctaaaaaag ctgtgggagt
     4201 ggtcaagcgt gaggtagtgc aagttgttac cccaggaacg gttgtggagt caacgaagcc
     4261 ggatagtgct aataatttct tagtagcgat tgattcgcaa gatcaacaaa catttggtct
     4321 agcatatatg gatgtctcaa ctggagagtt tcaggcaacc cttttaacag attttgagtc
     4381 cgtccgtagt gaaatactaa atttaaaagc tcgtgagatt gtagtaggat atcaattgac
     4441 ggacgaaaaa aatcacctac tgacgaagca gatgaacttg cttttatcat acgaagacga
     4501 acgacttaat gatattcatt tgattgatga gcagttaact gatttggaaa tatctgctgc
     4561 ggaaaaactt ttacaatatg tgcatagaac acaaaagcgt gaacttagtc atttacagaa
     4621 agtagttcat tatgaaataa aggactattt acaaatgtca tatgcaacga aaaatagtct
     4681 agatttactg gagaatgcta gaacaagcaa gaagcacgga agtctttact ggttgttaga
     4741 tgagactaaa acagcgatgg gaactcgaat gctgagaact tggattgaca ggcctttggt
     4801 aagtatgaat cgaatcaagg aacgtcaaga tattattcaa gtgtttcttg attatttttt
     4861 tgagagaaac gatctcacag aaagtttaaa gggtgtatat gatattgaac gcctagcaag
     4921 tcgagtatct ttcggaaaag ccaaccctaa agatctattg caactcggac agaccttatc
     4981 acaaattcct cggattaaaa tgattttaca gtccttcaat caacctgagc ttgacatcat
     5041 tgtcaacaaa attgacacta tgcctgaatt agaaagttta attaatacgg cgatagcccc
     5101 agaagcacag gctactatca ctgagggaaa cattatcaag tctggatttg ataagcaatt
     5161 ggataattat cgaacagtga tgcgtgaagg tacaggttgg attgctgata ttgaagctaa
     5221 ggaaagagca gcaagtggta tcggtaccct taaaattgat tataataaaa aagacggcta
     5281 ttacttccat gtgaccaatt ccaatttatc actagtaccg gagcattttt tccgtaaagc
     5341 gacattaaaa aattctgaac gctatggaac agcagaacta gccaaaattg aaggtgaaat
     5401 gctcgaagct cgcgagcaat cttcaaattt agaatatgat atttttatgc gtgttcgtgc
     5461 tcaagtagaa tcttatatta aacgtcttca agagttagca aagactattg caaccgttga
     5521 tgttctacag agtttggcag tagttgcaga aaatcatcac tatgttcgtc ccaaatttaa
     5581 tgatgaacat cagattaaga ttaagaatgg gcgtcatgca accgttgaaa aagtgatggg
     5641 agtgcaagaa tatattccca atagtatcta ttttgatagt cagacagata tccagttgat
     5701 tacaggacca aatatgagtg gtaagtcgac ctatatgcgc cagttagctc tgacagttat
     5761 tatggcacaa atgggaggtt ttgtatcggc agacgaagtt gatttgcctg tatttgatgc
     5821 aatatttact aggattggtg ctgctgatga cttaatttct gggcaatcaa cctttatggt
     5881 agaaatgatg gaagcgaatc aagctgtaaa acgagccagt gataaatctt tgattctttt
     5941 tgatgaatta ggtcgaggga cagccactta tgatggtatg gcattagctc aatcgattat
     6001 agaatatatt catgaccgtg ttagggcaaa aacaatgttt gcaactcatt accatgagtt
     6061 gacagattta tctgaacaat tgacaaggct tgtcaatgta cacgtggcta ctttagagag
     6121 agatggagaa gttaccttct tacataaaat tgaacctgga cctgcggaca agtcttatgg
     6181 gatacacgtc gcaaaaatag ctggtttacc aattgactta ttggataggg caactgatat
     6241 tttatcacag ttggaagctg atgcagtaca gttgatcgta tcagcccccc aagaagctgt
     6301 tactgctgac ttaaatgagg aactagatcc tgggaagcaa caaggacaat tatcgctttt
     6361 tgaagaacat tcaaatgcag gtagggttat tgaggagtta gaagcgatag atataatgaa
     6421 tctaactcca atgcaagcta tgaatgctat atttgattta aagaaattat tataaaatag
     6481 cctttaggca acaaaaaaga gactaaataa ttcatttagg ctctttttta attatttaac
     6541 aagattgata tttgttgctt gtggtccacg ttgaccatct tgaatatcaa atgttacttt
     6601 ttgtccttct tcaagagttt tgaagccatc aactttaatc tctgaaaaat gagcgaagac
     6661 gtctgttcca gtttcgctag aaataaagcc aaaacctttt tctgagttaa accatttcac
     6721 tgtaccttgt gtcatttgtt tattaccttt ctaaaaatgt tactgtaaaa aataaaaaag
     6781 gtaaaaatgt taaacaaaaa tacaacttca aaacttatta cgctccctaa gtctaacagg
     6841 taataggagt gatgtcaact aaaaattgtg ttattgtcaa attaatattt tgaatatggt
     6901 ataatggcta agatatcaca aaaaaagtta caaaaaatca taaaaaaagg agggatgaac
     6961 ttgtctaaaa ttattgaatt accggatata ttagctaacc agattgctgc tggtgaggtt
     7021 gttgaaaggc ctagtagcgt cgttaaagaa ttagttgaaa atgccataga tgcaggaagt
     7081 agccaaatta ccatagaggt tgaagaatct ggtcttaaaa agattcaaat aacggataac
     7141 ggtgaaggga tgactagtga ggatgccgtt ctcagtttac gacgtcacgc tacatccaaa
     7201 atcaaaagcc agagtgacct ttttcgtatt cggaccttag gttttcgtgg tgaagctcta
     7261 ccatcaatcg cttcgattag tttaatgaca ataaaaacag ctacagagca agggaaacaa
     7321 gggacgttac ttgttgctaa agggggaaac attgaaaaac aggaagttgt ttcaagtcct
     7381 cgtggaacaa aaattttagt tgaaaattta ttctttaata cgcctgccag attgaaatac
     7441 atgaagagtt tacagtctga actagctcat attattgata ttgttaatag actcagtcta
     7501 gcgcatccgg aagttgcttt tacactgata aatgatggta aagaaatgac taaaactagt
     7561 gggactggcg atttgagaca ggcgattgca ggtatttatg gtttaaatac tgcaaaaaaa
     7621 atgattgaga tttcaaatgc agatttagat tttgaaattt cgggttatgt aagtttgcct
     7681 gaattgacac gagctaaccg aaactatatt accctattaa ttaacggtcg ttacattaaa
     7741 aattttcttt taaaccgctc aattctagat ggttatggtt caaagttgat ggttggaaga
     7801 tttccaattg ctgtcattga tattcagata gatccttatc tagcagatgt caacgttcat
     7861 ccgactaagc aagaggtgag aatttctaag gaacgagaat taatgagttt aataagctca
     7921 gcaatttctg agagtctgaa acaatatgat ttgattccgg atgctttaga gaatttagcg
     7981 aaaactagta cccgaagtgt agataagcct atacaaacaa gcttctcact aaaacagtct
     8041 ggtttgtatt acgataggac taaaaatgac ttttttatag atgcagatac tgtatctgaa
     8101 cctattgcta actttacaaa ccttgacaaa agtgacggtt cagttgacaa tgatgtaaaa
     8161 aactctgtta atcaaggagc aacgcagtct cctaatataa agtatgctag tagagatcaa
     8221 gctgatagtg agaacttcat tcattctcag gactacctta gtagtaaaca gtcacttaac
     8281 aaacttgttg aaaaattaga ttcggaagaa agttcgactt ttccagagct cgaatttttt
     8341 ggtcaaatgc atgggaccta cttatttgct caagggaatg gtgggttgta tattattgat
     8401 caacatgcag ctcaagagcg tgtaaaatac gagtattacc gtgaaaagat aggtgaggtt
     8461 gataatagtc ttcaacaatt gttagtacca tttctatttg aattttcaag ttcagatttt
     8521 cttcaattac aagaaaaaat gtcattatta caagatgttg gtattttctt ggaaccgtat
     8581 ggtaataata ctttcatttt aagggaacat ccaatttgga tgaaagaaga agaggtagag
     8641 tctggcgttt atgaaatgtg tgatatgttg ttgctcacta atgaagtatc agtgaaaaaa
     8701 tatcgcgcag agttggctat tatgatgtct tgcaagcgat ctattaaagc aaaccatacg
     8761 ctagacgatt actcagcacg tcatttatta gatcagttag cgcaatgtaa aaatccttat
     8821 aattgccctc atggaagacc tgttttagtt aattttacaa aagctgatat ggagaaaatg
     8881 tttaaacgaa tccaagaaaa tcatacaagc ctcagggatt taggaaaata ttgataatta
     8941 cttgttaggg aagaagggag aaaaagtgaa agagtttttt gctttgccaa aacagctaca
     9001 gttaagagaa ttattgcgtt ttatttccat tacggttggt tcagctattt ttccttttat
     9061 ggcaatgtat tatgttcaat attttggaaa tctagttaca ggaattttga tcatcataac
     9121 tcaactatca gggtttgtag cgaccttata tggaggccat ttgtcagatg ctatagggag
     9181 gaaaaaagtt gtgattgtag gttcactgct ggcaaccatt ggttgggcta taactatagc
     9241 tgcaaatgtt ccaaatcata ttacgccaca cttgaccttt gtaggtatcc ttatcattga
     9301 aatagctcac caattttatt cccctgccta tgaagctatg accattgatt tgacgaacga
     9361 acagaatcgt cgctttgttt acactatagg ttattggttg gttaacatcg ctgtaatgct
     9421 aggttctggg attgctggga ttttctatga tcaccatttt tttgaattat taatagttct
     9481 tcttattatt tcagcgattt gttgtttggt ggtctacttc aagtttgatg agacaaaacc
     9541 acaggaggga accttcaagc atgataaggg agttttagga acttttaaaa attatagtca
     9601 ggttttagtg gataaagcgt ttgttgttta tactttagga gcaattggtt ccagtgttgt
     9661 ctggctccag gttgataatt atttctcggt caatcttaag caaaattttg aagtagtgtc
     9721 tgtgttagga catacaatca caggtgctaa aatgcttagt ttagcagtct ttacgaacac
     9781 tctattaatt gtcttactaa tgacaactat aaataaattc atagagaatt ggcctttgaa
     9841 acgtcaacta atattaggaa gcttgatttg tggttttgga atgcttttta atatttctct
     9901 aaacactttt ggagctattt tgattgccat gacgtttttc acctttggcg aaatgattta
     9961 tgttccagct agtcaggttc taagggcgga gatgatggtg gagggaaaaa ttggatcata
    10021 ttctggattt ttagctatcg cacaacctgt tgcaagtgta ttagcaggtg ccatggtttc
    10081 gctcagctat tttacaggaa aaataggtgt tcaaataaca ttgaccattt ttatgttagc
    10141 aggtctggtt ttaatcttat acgctaccaa aatgaaaaat attgagatag gaaaatagaa
    10201 tgtacgatta tattaaagga aaattgagta aaataacggc taaatttatt gttgtggaaa
    10261 cagctggatt gggatacgtg atatatgtgg caaatcccta ctctttttca ggttacgtta
    10321 atcaagaagt taccatctac ttacatcaag tcattcgcga tgatgcacat cttttatttg
    10381 gttttcatac ggaaaatgaa aaagaaattt ttcttaattt aatttcagta tcaggaattg
    10441 gtccaacgac cgctttagca attattgcag tcgatgacaa tgaaggtctc gtaagtgcca
    10501 ttgataatag tgatattaag tatttgacta aatttccaaa gataggaaaa aaaacagcac
    10561 aacagatgat ccttgatttg tcagggaagt ttgtagaagc ttctggtgag agtgcgacta
    10621 gtcgtaaggt tagctctgag cagaatagca atcttgaaga agctatggaa gctttgttag
    10681 cgttggggta taaggcgact gaattgaaaa aagtaaaagc cttttttgaa ggtacgaacg
    10741 atactgtgga gcaatacatc aaatctagtt tgaagatgtt gatgaaataa tttagaagta
    10801 atgaggtttc acatgaaacg atgttcttgg gtgaatttag ataatccatt atatgtagct
    10861 taccatgata aagagtgggg gagagctgtt catgacgatc atgtactctt tgagttacta
    10921 tgtttagaaa cttatcaatc gggtttatct tgggaaacag tattaaataa acgccaggag
    10981 ttcagacaag tttttcatca ctataatatt gaaaaggtag ctgcaatgtc agatgcggat
    11041 ttagagataa tcttacaaaa tcctagggtt attcgtcatc gtctaaaact tttctcaaca
    11101 cgacaaaatg ctaggtcaat tatattaatt cagaaagaat tcggaagttt tgatcgctcc
    11161 atttggtctt ttgtagataa taaagtacag gttaactcag ttaacaacta caacgatgtg
    11221 cctgcaagta ctaccttatc cgaacgctta tctaaggatt taaaaaaacg cggttttaaa
    11281 tttgttggtc caacttgcct ctattctttt attcaggccg cgggtatggt taatgaccat
    11341 gaaaatatat gtgatttcaa gtaatatcaa tgtgatatat tttaagtagc acttctgcta
    11401 ctttttttgc taaaatgtag tcattatttc gaatagataa gagggtgaag ttgtgagggc
    11461 tgaaattatc gctgtaggaa cagaaatttt aacaggacag atcgttaata caaacgcaca
    11521 atttttgtcg gagaaaatgg cagaattggg aattgatatt tatttccaga cagctgtagg
    11581 agataatgag gaacgtttat tatcgctttt agatatagct agtcagcgca gtcaaatggt
    11641 tatcctatgt ggtggacttg gacctacaga ggatgattta acgaaacaaa ctttggctaa
    11701 atttttaggt aaatcattaa cagtggattt attagctagt caaaaattgg atcgtttttt
    11761 tgcaagtcgt cctcagtttg ctagaacgcc aaataatgaa cgacaagctc aattggttga
    11821 aggctcaata cctctacaaa accttactgg tctagctgtt ggtggtatcg ttacatctaa
    11881 gggagtccaa tatatggttt taccaggtcc gcctagtgaa ttaaagccaa tggttatgga
    11941 gcaggtggta cctattttgt caaataatgg cacaaaatta tattcacgtg tattgagatt
    12001 ctttggtatc ggtgagagtc agttagtcac aatcttagaa gacataatta agaatcagac
    12061 agatccaact atagcaccat atgcaaagat tggtgaggta actttaaggt tatcaacgaa
    12121 agctgaaaat cagaatgaag ccgactttaa attagatagc ttagaaaaag aaatacttgc
    12181 tcttaagaca ttagataacc gtaaattaaa agatttatta tatggctatg gtgataataa
    12241 tagtatggca cgtactgttt tagaattatt gaaagtacaa aataaaacga ttactgccgc
    12301 agagagctta acatcagggt tatttcaatc tcagttagca gaattttctg gtgcttcgca
    12361 agtttttaat ggtggcttta cgacctatag tatggaagca aaatcacaac ttctcggcat
    12421 ccctaagaaa aaattgcaag aatatggagt tgtcagtcat tttacagctg aggcaatggc
    12481 tcagcaagct cgccagcttt taaaagcaga ttttggtatc ggtttaacgg gagttgctgg
    12541 tcctgatgag ttggaaggat accctgcagg tacagttttt attgggattg caacccctga
    12601 aggcgtttct tctattaaag tatcgattgg agggaagagc cgctcagatg tccgtcatat
    12661 cagtacttta catgcttttg acctggtgcg tagggcttta ttaaagatat aatatttgtt
    12721 ataattagta gaacgatttc taaatttgaa tcaacctaga ttcatagaaa tataaggaga
    12781 agacattggc taaaaaaacg aaaaaagcag aagaaattac taagaaattc ggtgatgaac
    12841 gtcgaaaagc cttagatgat gctttgaaaa atattgaaaa agattttggt aagggcgcag
    12901 tgatgcgctt aggtgagcga gctgaacaaa aggttcaagt aatgagttca ggttctcttg
    12961 ctttagatat cgctttagga gcaggtggat atcctaaagg acgtatcgtc gaaatttatg
    13021 gaccagagtc atctggtaaa acaacggttg cccttcatgc cgtagctcaa gcccaaaagg
    13081 aaggtgggat tgctgccttt attgatgctg agcacgctct tgacccagcc tatgctgcgg
    13141 ctcttggagt taatattgat gagttactct tgtctcaacc tgactcaggt gagcaaggtc
    13201 ttgaaattgc aggtaaattg attgattcag gcgcagttga tttagttgtt gtcgattcag
    13261 ttgcagccct tgttcctcgt gctgagattg atggtgatat tggtgacagc cacgttggtt
    13321 tgcaagctcg tatgatgagt caagcaatgc gtaaattgtc agcatccatt aacaagacaa
    13381 agacaatcgc aatttttatc aaccagctaa gggaaaaagt aggcgttatg tttggtaacc
    13441 ctgaaacaac acctggtggt cgtgctttga aattttattc atcggttcgt ctagatgttc
    13501 gtggtaatac tcaaatcaag gggactggtg agcataaaga tcataatgtt ggtaaagaga
    13561 caaaaatcaa agttgttaaa aataaagttg ctccaccatt ccgtgaagct tttgtcgaaa
    13621 ttatgtatgg tgaaggtatt tctcgcacgg gtgagttaat taaaattgct agtgatttgg
    13681 atattatcca aaaagcaggt gcatggtact catataatgg tgagaaaatt ggacaaggtt
    13741 ctgaaaatgc caaaaaatat ttagcagata atccagcaat ttttgatgaa attgaccata
    13801 aagtgcgcgt gcactttgga atgactgaag atgattcgcc agttcagtct gagctcgttg
    13861 aagaaaaaaa tgaagctgat gatcttgttt tagatttgga caatgctatt gaaattgagg
    13921 aataaaatag caactataca tcttgaactt gaataaatgt aactaaaata agttctgaca
    13981 gtggttggaa cttattttta t
//
LOCUS       DATRNX010000046        13894 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRNX010000046 DATRNX010000000
VERSION     DATRNX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            105..986
                     /locus_tag="VBC81_001126"
     CDS             105..986
                     /locus_tag="VBC81_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6912175.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            1001..1846
                     /gene="pstC"
                     /locus_tag="VBC81_001127"
     CDS             1001..1846
                     /gene="pstC"
                     /locus_tag="VBC81_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6912176.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            1848..2726
                     /gene="pstA"
                     /locus_tag="VBC81_001128"
     CDS             1848..2726
                     /gene="pstA"
                     /locus_tag="VBC81_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6912177.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            2719..3468
                     /gene="pstB"
                     /locus_tag="VBC81_001129"
     CDS             2719..3468
                     /gene="pstB"
                     /locus_tag="VBC81_001129"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6912178.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            3465..4121
                     /gene="phoU"
                     /locus_tag="VBC81_001130"
     CDS             3465..4121
                     /gene="phoU"
                     /locus_tag="VBC81_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6912179.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            4121..4798
                     /locus_tag="VBC81_001131"
     CDS             4121..4798
                     /locus_tag="VBC81_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912180.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            4791..6446
                     /locus_tag="VBC81_001132"
     CDS             4791..6446
                     /locus_tag="VBC81_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6912181.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            6601..8784
                     /locus_tag="VBC81_001133"
     CDS             6601..8784
                     /locus_tag="VBC81_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6912182.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            8823..9653
                     /locus_tag="VBC81_001134"
     CDS             8823..9653
                     /locus_tag="VBC81_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6912183.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            9877..10629
                     /locus_tag="VBC81_001135"
     CDS             9877..10629
                     /locus_tag="VBC81_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912184.1"
                     /translation="MNIFILEDNFIQQARLERIIEEVKIRRSIHSKMVNTFEKPVQLL
                     ESICEKGMHNLYFLDIEIKNDEQRGLEVAKQIRQIDPYAQIVFVTTHSELMPLTFRYQ
                     VSALDYIDKGLSQEAFSRRIEDVLLYANNACSKNLLEDSFYFKSRYSQVQVPFNDILY
                     IETSLRSHRVVLYTAKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRSNKL
                     IYLKNGSNCLISRNKIKDIMVVIEENQRGRGI"
     gene            10626..11960
                     /locus_tag="VBC81_001136"
     CDS             10626..11960
                     /locus_tag="VBC81_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6912185.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(12031..12213)
                     /locus_tag="VBC81_001137"
     CDS             complement(12031..12213)
                     /locus_tag="VBC81_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912186.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYLLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            12502..12675
                     /locus_tag="VBC81_001138"
     CDS             12502..12675
                     /locus_tag="VBC81_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6912187.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            12744..13592
                     /locus_tag="VBC81_001139"
     CDS             12744..13592
                     /locus_tag="VBC81_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912188.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            13598..>13894
                     /locus_tag="VBC81_001140"
     CDS             13598..>13894
                     /locus_tag="VBC81_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912189.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYY"
BASE COUNT         4438 a         2170 c         2748 g         4538 t
ORIGIN      
        1 tccaactctt tttaatttta cattgatttt acaaaaatcc tcaaagagat ttgatattcc
       61 ttaactataa taaaatcaaa tcaaaaaata tttaggagtt catcatgaaa aaacacaaaa
      121 tgctatctct tttggcggta tctggtctta tgggaattgg catcctagca ggatgctcaa
      181 atgattcatc atcttcatct aaagggacaa ttaacattgt ttctagagag gaagggtctg
      241 gaactcgtgg agctttcata gagttatttg ggatcgagtc aaaaaataaa aagggcgaaa
      301 aagtagacca tacttctgat gcagcaactg ttaccaattc aacttcagtt atgctaacca
      361 ctgtctctaa agacccgtca gctattggtt attcttcact aggttcatta aatagtagcg
      421 ttaaagtgct taaaattgat ggcaaaaatg cgactgtaaa agatattaaa tcaggaagct
      481 ataagatttc tcgtcctttc aatattgtaa caaaagaagg taaggaaaaa gaagcaacaa
      541 aagattttat tgattatatc ttaagcaaag atgggcaagc agtcgttgag aaaaatggct
      601 atattccact tgacaatgct aaagcatatc aagcaaaagt gtcatctggt aaagttgtta
      661 tagcaggttc aagttcagta acgccggtta tggaaaagat taaagaagcc tatcataaag
      721 taaatacaaa agtagatgtt gaaattcaac aaagtgattc ttcaactggg attacaagcg
      781 caattgatgg atcagcagat ataggtatgg catcacgtga attggataag acagagtcat
      841 ccaaaggggt caaagctact gttattgcaa cagatggtat tgcagtagtt gttaataaga
      901 aaaataaagt taatgatcta tcaacaaaac aagtaaaaga catttttact ggaaagacaa
      961 cgtcttggtc tgacttatca aagtaaggta agagtaaaca atgaaacaaa caaaagaagg
     1021 tcttatgcaa gctgtgtttt tcatcacggc ttgcgtcagt gttatatcag ctattttaat
     1081 ttgcctcttt ctcttctcta gtggtcttcc agctattaca aaaatcggct ggggaaattt
     1141 tatatttggt aaagtctggc atcctagtaa taatattttt ggcatctttc caatgattgt
     1201 aggttcctta tatgttacag cgggagcttt acttttagga ggcccgatag gtatccttac
     1261 tgctgttttt atggcttatt tctgcccaga aaatatttat aggcctttaa agtcagctat
     1321 caatttaatg gcgggaattc cttcagttgt ttatggtttc tttggactcg ttgtcattgt
     1381 ccctatgata aggcaatata ttggtggctt tggtatggga gtcttagcag cttcaatttt
     1441 gctaggaatt atgattttac caacaattgt tagcatttca gaatcttctt taagagctgt
     1501 tccagagagc tattatgaag gcggtattgc ccttggtgcc tctcatgaac gaagtgtctt
     1561 ctttgctgtt ttaccagctg ctaaacgtgg tattttagca tcggttgttt tgggaattgg
     1621 acgtgctatt ggtgaaacaa tggctgtcat catggtagcg ggtaatcaag ctgtcttgcc
     1681 tcaatcgctg acatcaggcg ttagaactct aaccactaat attgtcatgg aaatgggata
     1741 ttcttctggt ttgcatcgtc aagccttaat tggtacagct gtggtactct ttatctttat
     1801 attaatgatt aatatcagtt tttcagcatt acagaaagag gaataagatg gaaaatatta
     1861 accaaagaac gtcaaaacaa tatttagcat ctttaaaaaa agatcccctt tccttctttc
     1921 tttttgctat tgtctatttg ggagcaattc taagttttgc tacaattgca tttgttgtta
     1981 tttatatttt agttaagggg cttcctcatg tgaatactgg cttgtttgca tggacttata
     2041 atactcagaa cgtctcacta ttaccagctt tcattaacac tatctttatc atcgccttaa
     2101 ctttattatt tgcagttccc cttggtattg gtggatcaat ttatctaacg gaatatgcta
     2161 gacgtgataa tccttatctc aaaattatcc gtgttgcaac cgagacattg gcagggatac
     2221 cttcaattat ttatggttta tttggtgcat tattttttgt aaaatacaca cacttaggct
     2281 tatctttgat atcgggttct ctgactttga gcatcatgat tttacctttg attatgagga
     2341 caactgaaga agctttacta tctgttccgg attcttatcg agaaggagcc tttgcgcttg
     2401 gtgctggtaa attgagaacc atatttaaaa tcgtattacc tagtgctatg tctggtattt
     2461 ttgctggtat tattttagcg ataggacgta tcattggtga gtcagcagca cttattttca
     2521 cagctggaac agttgcaaaa gtagtacatt cggtatttag ttcatctcgt actttagcag
     2581 tacatatgta tgctatttcg ggtgagggct tatatgttga ccaaacttat gcgactgcgg
     2641 tcatcttatt gcttttagtt attattgtca attttgtatc tggactagta gccaaacgat
     2701 taggaagtga gtcgaacagt gagtaaatta gtaattaata atttagattt gtattatgga
     2761 gaatttcacg ctctgaagga tgttaattta gacattgaag aaaaagaaat cacagccttt
     2821 attggacctt ctgggtgcgg aaagtctact ctcttgaaaa gtattaaccg tatgaatgat
     2881 ttggtcaaaa attgcaaaat cacaggggat attactttag aaggcgagga tgtctataga
     2941 caattggata ttaaccaatt gcgtaaaaag gttggaatgg tgtttcaaaa acctaatccg
     3001 tttccaatga gcatttatga taatgttgct tttggtccaa gaacgcacgg tattcattcc
     3061 aaagcagagt tagatgatat cgttgaacgt tcccttaaac aggcggctct atgggatgag
     3121 gttaaagatc gtcttcacaa atctgctctg ggtatgtctg gcggacagca gcaacgtttg
     3181 tgtattgcgc gtgctcttgc tattgagcct gatgttttac ttatggatga acccacaagt
     3241 gccttggatc ctatttcaac agcaaaaatt gaagaattgg ttatccaact gaagaagaac
     3301 tacactattg ttatagtgac acataatatg caacaagctg ttcgtatttc tgataaaaca
     3361 gcattctttc tgatgggaga agtggttgaa tataacaaaa cgagccaact cttttcattg
     3421 ccacaagatg agcgtacaga aaactacatt acagggagat tcggatgatt cgttcacgat
     3481 ttgcaagtca acttaatgac cttaataaag aaattatttt tatgggggca ttgtgtgagg
     3541 acatcattgg gaagtcctta ggtgctctaa caaatagtaa tgatgtttac cttgacgata
     3601 tttctgaaac ttatcacaag attgaacaaa tggaacggga tattgaggag agatgtttaa
     3661 aacttttact tcgtcagcaa ccagttgcca aggatttacg ccgtatttct tctgcattaa
     3721 aaatggttta tgatatgaaa cgtattggtg ctcaagctta tgaaattgcg gaaattgtta
     3781 gtttaggtca tattatccaa ggtgcaggtt ctgaaaggga tcaacttaac agcatgtcta
     3841 ataatgtcat tagtatgctg actaagagta tagatgcttt tatttatgac aatgaagaac
     3901 aagcgcatca agtaattgaa caagatagga cagttaacca ggaatttgat acaataaaga
     3961 aacagttagt gttatatttt tcggtacagg atgttgatgg tgaatatccc atagatgttt
     4021 taatgatcgc caagtaccta gagcgtattg gtgatcatac agtaaatatt gctaaatggg
     4081 tgcttttctc aataacaggt aacctagatg gagaagcgta atgatatatt gtgttgaaga
     4141 tgatgctgat attcgcgaaa tgatgctcta tactttgcaa atggcaggtt ttacagctca
     4201 aggattctcg agttcagagc ttttttggga agctgttcag gagaaagttc cagacttgat
     4261 tttacttgat attatgttgc cgggagatga tggtttgact attttagagc gtttaaggag
     4321 aaaacatcaa acggaaatga taccagtaat catgacaact gctaaaggta gtgagtatga
     4381 taaagttaaa gggttagatc ttggagcaga tgattatctc gtcaaacctt ttgggatgat
     4441 ggaaatgatt tcacgaataa aagcagtctt gagacgtagc cgccaagtag attcaaaagc
     4501 tcatattatc attggaaatt tagagattga cccgactaat tattgggtca aaaggggaac
     4561 ggaaaaaatc cacttaaccc taaaggaatt tgagttatta gtattattct tccgtaatcc
     4621 caatagagtc tttacaaggc aagaacttct ggataaggtt tggggagaac aatttttagg
     4681 agaaactaga actgtggatg ttcacattgg gacactgcga acaaaacttg gtgaggatgg
     4741 ctatttgatt gctacagtaa ggggagttgg ataccgtttg gaggaaagac atgactaaaa
     4801 aaattttccg tacaacttta tccgctagtt tgggaattgt tttagttaca attttgatga
     4861 ttatgggatt tctctataat tacttcaatc gcattcagcg agaacagttg agaacacaaa
     4921 cggcactggc gtcacaagga atttcctttg aaggtaaaga ttattttgaa aatcttaaaa
     4981 cttcaaatgt gcgtattact tgggtagata ataagggaca agtgctttat gatacccaat
     5041 cagatgcaaa acatatgaaa aatcacgcca atcgtcaaga aattaaagaa gctatcaaga
     5101 gtggttatgg tgagagcacg aggtggtcag cgacacttac tgagaagtct atctatgctg
     5161 cgcaacgttt aaataatgga acgatagttc gtttatcagt tgcgcaacaa actatttttt
     5221 atcttctcct tggtatgatg agtcccttag caattatcat cttacttgct attattttat
     5281 ctgtattaat tgcacgatat atagcaaaaa aagtatcaga gcctttaaac aatattgact
     5341 tagatcatcc tttatcaaat gatagttacg aggagataac accattatta aggcgtcttg
     5401 atagccatca ggctaagatt caacatcaaa aactgttatt acaaaagcgt caaaaagaat
     5461 ttgataccat tatttctaaa attaaggaag gaatgattct tttagatgat caagcaagaa
     5521 tcgttagtat caacgcagaa gctttaaagt tattccaaat taatgatgat tggcatgggc
     5581 gctttatgat ggaagttagc cgagatttaa ctctcaaaga cttaattgat caaggactaa
     5641 aagggaaaaa gaaagaggct aatattgaca ttgaaaataa tcattaccgc gtcttggtaa
     5701 gaccaactac agataacaac cgagtaacag gattggttgt tctactgttt gatgtgactg
     5761 accaactcca aatggaacaa cttcaacgtg aatttactgc caatgtctct catgaattga
     5821 aaaccccgct tcatgttatt tctggttact cagaactgtt agctaatcaa atggttccaa
     5881 acgaagaagt cccacaattt gcagctaaaa ttcataaaga atctgaacga ttagttaaat
     5941 tggttgagga tattatcaac ctttctcacc ttgatgaaca agaaaaatta ccgcaagaga
     6001 cagtaaatct gtatgactta acacaaaaag ttttagaagg tttacaagct aaagcagata
     6061 agaagcacat acaaatcaat tttaatggag aagaagctat tttgcgaggg aatccagttt
     6121 tattgaattc cttggtgtac aacttgtgtg acaatgctat cacctataat catgagaaag
     6181 gtcaagtaaa tgtgactttg aaaaattcac ctgatacaat taccttagaa gttagtgata
     6241 cgggactagg cattgcagag aaggacaaaa aacgtatttt cgaacgtttc tatcgcgtcg
     6301 ataaaagtcg ctcaaaaata gttggaggga ctggtttagg tttgtctatt gtaaagagtg
     6361 ctttagattt tcacaatggt agtataaaag tggacagcca tttggggcag ggaacaacaa
     6421 tgaccgtttt acttcacaaa caataaaaac tcacaaataa aagtctagat gatattatct
     6481 agactttttt agtaattcag aaaaaatata taagtaaggg tttacaaaaa acaaataagt
     6541 gctataataa aaccataacg caaacgtttg cactattaag tgcataaaag gagattgaag
     6601 atgaaaaata atgtcaaaca gctctttagt tttgaatttt ggcagaagtt tgggaaggct
     6661 ttgatggttg ttattgctgt tatgccagca gcaggattga tggttagtat tggtaactcc
     6721 attaggctat tggatccatc aaatgtttta ttagggcgca tagctaatgt tattgctcaa
     6781 attggttggg gagttattgg taacttacac atcttatttg cattggctat tggtggtagc
     6841 tgggctaaag aacgtgccgg tggtgctttt gcggctggct tatcctttat tcttattaac
     6901 cttattacag ggaacttttt tggtgtgaaa acagatatgt tagcaaacag taaagcaact
     6961 gttcaaacgg tatttggtgc gaccatcaga gtttcagatt actttgttaa cgttcttgga
     7021 caacctgctc ttaatatggg tgtatttgtc ggtattattt caggtttcgt tggtgcaact
     7081 gcctttaata aatattataa ctatcgtaaa ttaccagatg ctttaacttt tttcaatgga
     7141 aaacgttttg ttccatttgt tgttatttat cgttcagtca ttgttgcatt aattttatca
     7201 gtattttggc cggttgttca atcaggtatc aatggctttg gtaagtggat tgcaagttca
     7261 caagacagtg ctccaatatt agcaccattt gtctacggta cgttagagcg tttattgctc
     7321 ccatttggtt tacaccatat gttaactatt ccaatgaact acacgcaact aggtggcaca
     7381 tacactgtcc ttacaggtgc aactaaagga gcacaggtgt tgggacaaga tccactgtgg
     7441 ttagcatggg ttggtgattt aatcaactta aaaggctcaa attcaagtca ataccatcac
     7501 ttattgacaa gtgtcactcc agcacgtttt aaagtggggc aaatgattgg tgcttcaggt
     7561 attttgatgg gattatctta tgctatgtat cgtaacgttg ataaagataa aaaattgaaa
     7621 tacaagtcaa tgtttatttc agcagcagcg gctactttct tgacaggtgt tactgagcca
     7681 attgagtaca tgttcatgtt tgcagcaatg ccactctacc ttgtatatgc tgtcgttcaa
     7741 ggatgtgcgt ttgctatggc agatattgtt aacctccgcg ttcattcatt tggtaatata
     7801 gaatttttaa cacgtgttcc catgggaata aaagcgggcc ttggtggtga tattttcaac
     7861 tttgtttggg tgacacttct ttttgcagta ttgatgtact ttattgcaaa ctttatgatt
     7921 aaaaaattca atttagcaac agcaggacgt aatggtaatt atgataacga agaggttgat
     7981 aatgctcctt caacagcttc tggttctgct gatgcaaatt ctcaagttgt acaagttatt
     8041 aatttacttg gtggtcgtga taacattgaa gatgtagatg catgtatgac tcgtcttcgt
     8101 gtcacagtta aagatgggaa tagcgtgggt tctgaagcag cttggaaaaa agctggtgca
     8161 atgggattgg tgttaaaagg taatggcgtt caagctattt acggacctaa agcagatgtc
     8221 cttaaatcag acattcaaga tttgctagat tcaggtacag tgattccaat agttgattta
     8281 gaaacaggcc agcctgtagc agcaacccaa gttacaactt ataaaggaat aacagaagaa
     8341 attgtttcgg tggctaatgg tcaagtagaa gcattggatg ttgttaaaga ccctgtcttt
     8401 tctcaaaaaa tgatgggtga tggctttgct gtggaaccaa ctgatggcaa tatctatgtt
     8461 ccagtttcag gtacagttac tagtgtcttt ccaacaaaac atgcttttgg tttattaaca
     8521 gaaagtggcc tagaagtttt agtacatatc ggtctagata cagtagctct tgatggtcaa
     8581 ccatttgagg ttaaaatttc tagtggtcaa aaagtggtag caggcgatct tgctgtagta
     8641 gcagatttgg aagctatcaa agcagctggt aaagagacaa gtgtgattat cgtctttaca
     8701 aatgtttcag atataaaaac tgttaaactt gaaaaatcag gtccacaaat agctaaaaca
     8761 gttgtggcaa aagtcgaact gtaacaaacg ataggagctg ggcaagtctc agcttctttc
     8821 tgatgaaagg atactctatg gcaaaatgct taacgttaaa tactcattca tggatggaag
     8881 taaatgctct aaagaaatta tttgatttag cagagcatat cttccgtgaa aaatatgata
     8941 tcatctgcct acaagaggtg aaccagagca tatcttcccc tcttgctacg agtagtccaa
     9001 attatcatcc gattgaaggt acaccagctc ttcatcaaga taactttgcc ttgcaattag
     9061 tgcattacct aaatcttcaa ggtctacact atcattggac atgggcttat aatcatattg
     9121 gttatagtaa gtaccatgaa ggggtagcga tactttctct gaaaccatta aagccagatg
     9181 atattttagt ttcagctgta gacgatgaga ctgattatca tactcgtcgt gctctggtag
     9241 ctgagaccac tctgaatgac aaagtcgtta cagtagttag cctccattta tcatggtttg
     9301 agaaaggatt tgcggaggag tggaaacgac tagaaactac cctacttgag gtagaaactc
     9361 cacttttact catgggtgac tttaataatc ctactggaaa tcagggttat gaacttgttc
     9421 ttaatagtcc attagcatta aaggatagcc accaaatagc caatcatgta tttggagacc
     9481 atactattat ggctgatatt gatggttggg aagggaataa aaaggctctt aaagttgacc
     9541 atatttttac tagcgaagac ctttcaatca gttcaagtca agtagttttt gaaggtggtg
     9601 aagcacctgt cgttagtgat cattacggct tggaaattac gttaaatctt tagattaatc
     9661 tatttaatat attaaaagca tcctaaattt gtctataaaa caaaacttag ggtgcttttt
     9721 gaaaaatatg tcacattatt taacttctta cgaagtttgg aatgcatgag aaaatatgat
     9781 aaaatgataa aatgataata actattatag gtgattttaa taaataattt aatcaattgg
     9841 tttatctatt agaacaatta atacgaggag acgagagtga atatttttat tttagaggat
     9901 aattttattc aacaggcacg tttagagaga ataattgagg aagttaaaat taggagaagt
     9961 atacacagta aaatggtaaa tacctttgaa aaaccagttc aattattgga atcaatctgt
    10021 gaaaaaggta tgcataattt atatttctta gacattgaaa taaaaaatga tgagcagagg
    10081 ggcttagagg tcgcaaaaca aattagacaa attgatcctt atgctcagat tgtttttgtt
    10141 acaactcatt cggaattaat gcctttaacc tttcgttatc aggtatcagc tttagactat
    10201 atcgataaag gactttcaca ggaagcattt agcagaagga ttgaagacgt tttactatat
    10261 gctaataatg cttgcagtaa aaacttacta gaagattctt tttattttaa atcacgctat
    10321 agtcaagtac aagtaccttt taacgatatc ctttatatag aaacgtcatt aaggtcacat
    10381 agagttgtcc tttatactgc aaaagacaga atggaattca cagccacttt gggggatatt
    10441 ttaaaacaag aacctaggct ctttcaatgt catcgctctt ttttagtaaa tcccttgaat
    10501 atttttaaag tggataggtc aaataaacta atctatttaa aaaatggttc gaattgtttg
    10561 atttctcgta ataaaataaa agatattatg gtagtaatag aggaaaatca aagaggtaga
    10621 ggaatatgaa ttctactcta tccatcttag agatagcttt aacgataata ttaacaatat
    10681 ttctcttttc taaaatcagt ggacgtcaat tgactttaat agagattgga atttgtattg
    10741 ctctgagatt aggacttgta attttattta tagcagctaa tactattatt aatctttcat
    10801 ttctaaagta ctggcttctc cccatctata tgttaagtct ggctttcttg ttgctaagac
    10861 ctatatctaa aactttagta gtcttttatg gcttgtttcc aattgttctc agaaatcttt
    10921 gttcacgttc tttagctttt tttgtctttc ctttgtttaa tattgatttt catacaacca
    10981 accaggggtt ttggggatat tttgttgatg tcttgtctac tctttgtgtt ctcctatttt
    11041 taagatggat gcaatacgac tttaaaactt tgcgaagtag tgaagtttcg cccatagata
    11101 agagaattct ccaatttacg aatggttgta tggtcagtta ttacttactg atgcagtttt
    11161 caacttattt ggaatatgaa ttagccatca atactgttat ttaccggcaa tttttgcttg
    11221 ttgtttattg gattatattt acgggagcaa tagccagttt agaccggaga cttcgaaatt
    11281 ctttacagga gaggttagct gttcaaagag aactccaatt ggctaatatg atagattata
    11341 gccaccatat tgaggggttg tataaagaag ttaggagctt ccgacatgat tatagtaaca
    11401 tgttaacgac cttgaaacta gggattgata atgaagatat atctattgtc aaatcagttt
    11461 atcagtctat ctttaaagaa tctaatacac atttccgaca tcctaagtat aatatagacc
    11521 ggttggttaa tgtagataac ccagccttaa aaagtctttt agcagccaag ctttcacaag
    11581 cgatagacaa tcatattatg gtatctatag aagtaccaga cagtattaaa cctcaaggga
    11641 tggaactgat tgattttatt accattgtat ctgttttatg ggataacgct attgaggcat
    11701 ctttagagac agacgatcct aagatagcca tgtcttattt tacattaaaa cataaacaaa
    11761 tttttatcat agaaaattca actaaggaaa aggcaataga tacgtcaacg atattcaatt
    11821 atgagataag tagtaagggg cggaatagag gaataggtct ttacaatgtc aggggagtta
    11881 tcaatcgtta tccaaatatt tctattgcaa caagtagcca agattttagt ttctgccaaa
    11941 ctttcgaaat catattatag ttttaaaaag ttcaatttat ctaagtttat tattttctta
    12001 ataccatcaa tccaaccacc accagtgata ttatttagtt gttccggcga taattcctga
    12061 agctgtagtt ccatctcgat tagttttggc tagttctcgt aagtggttta ataaatattt
    12121 tgaaccatag aactttgcga ttgaagcaag tgcagttact ccacaatcct tagtatcaac
    12181 ctgacgcacc aatgcttttt tgtacgtcaa cattgtgtaa ttcctttctt cgttatcgta
    12241 ttaatatttt gccataaaaa tatatactta ttaaaaatag cttaaacagc tattgcaacg
    12301 cgtccaatgg tttctataag gatataaagg tagatagtac ttttctgttt tgaggcgatt
    12361 caacgacatt tcagggaata aacgtttcta tttttatttg aatgctatat taaatgtgtc
    12421 aagttgcaac agacaaaaat tttatatagg agatattcaa aataaatgta aaatcaattg
    12481 aacaattaga agttatggat aatgaagcat tatcacatgt tgaaggtggc cttggtcata
    12541 gatgggtata cagtaagaac cggtaaacat acttatacaa gtgcatggca tctttctgaa
    12601 aatactgctg cagcaaatgc aagaaatcgt atgggccttt accctggaag cgtttgtcgt
    12661 gtctatcttg tataattgat tttttattta agcgataggg aatcttcctt gccgcttaag
    12721 tttgtatagg gagagacaat actatgttat tacagctaga taatatcact aaaagctacg
    12781 gaaaaaaaat agtacttaac cagatttcct accagtttac tcctggtttg tatggactct
    12841 taggagcaaa tggtactggc aaaacaacct tattaaattt gatgagccac tttacattcg
    12901 cagatagtgg gaatatttat tggaatggac aggagcagtc ggaagaattt tatcgtcaca
    12961 ttggtttttt accgcaacat tttcgctatt atgatcaatt tactggcatt gctttcttaa
    13021 attatatcgc taccttaaaa ggtgttgata aaaagaaagc taaaccagaa attcctagat
    13081 tactagagct tgttggttta ggtgatgtag gtaaaaagaa gatttcatca tattccggtg
    13141 gtatgaagca acgtttaggc attgctcaag cacttatcaa tgaccctgaa atacttattc
    13201 tggatgaacc aactgttgga cttgatccta aggaacgggt taaatttcgc catattttga
    13261 gtcagttatc caccaataaa attatcattt tatcaacgca tattgtgtcg gatgtggaag
    13321 ctgttgccaa agaaattata gtattaaaaa atggtaaatt tatcgaacag ggcaacaccg
    13381 cacagttgtt gaaaactatt gagggaaaag tgtgggaaat aacgactgaa ccagatttat
    13441 ctcagatacc caatattgct attgttaatg aaaaagtcat ttcagatagt ctggtatttc
    13501 gtgtagtgag tgatatttgt ccgagtgatt cggcacagtt ggttgttccg actctagaag
    13561 atttctacat ctatcatttt agaggggagt aagtaagatg atatcaattt ttccttttga
    13621 attaaaaaaa cttctacaac gtaaggcagc attgggggct tttattatct tccttcttgc
    13681 tatttttggt ctattttacc agcatttttt tgttgggcaa attagtggct attctgcaga
    13741 taaaatacat ggtcgagaag cggttgcaat taatcgtaga atagctgaaa aatattcagg
    13801 ggatttatct gaagaacgag taacaaatat tattaaaaac tatgttaatc cgccagcagg
    13861 acgcccccct atatttgatg tttttagtta ttat
//
LOCUS       DATRNX010000047        13881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRNX010000047 DATRNX010000000
VERSION     DATRNX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..469
                     /locus_tag="VBC81_001141"
     CDS             <1..469
                     /locus_tag="VBC81_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO6912190.1"
                     /translation="CWLCAGTLRNFIWNKLSGINETLTSDIDVVFFDKNISYEETVVL
                     EQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTSSKDAISKFPEKCTAVGERLDDRNQL
                     ELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYLKRVDKKKWNNIWPRLTIIKK"
     gene            689..1108
                     /locus_tag="VBC81_001142"
     CDS             689..1108
                     /locus_tag="VBC81_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6912191.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1308..1787
                     /locus_tag="VBC81_001143"
     CDS             1308..1787
                     /locus_tag="VBC81_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6912192.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            1909..2601
                     /locus_tag="VBC81_001144"
     CDS             1909..2601
                     /locus_tag="VBC81_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6912193.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2598..3350
                     /locus_tag="VBC81_001145"
     CDS             2598..3350
                     /locus_tag="VBC81_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6912194.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            3347..3922
                     /locus_tag="VBC81_001146"
     CDS             3347..3922
                     /locus_tag="VBC81_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO6912195.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4017..5099
                     /gene="hrcA"
                     /locus_tag="VBC81_001147"
     CDS             4017..5099
                     /gene="hrcA"
                     /locus_tag="VBC81_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6912196.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKESEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            5102..5674
                     /gene="grpE"
                     /locus_tag="VBC81_001148"
     CDS             5102..5674
                     /gene="grpE"
                     /locus_tag="VBC81_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6912197.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5855..7684
                     /gene="dnaK"
                     /locus_tag="VBC81_001149"
     CDS             5855..7684
                     /gene="dnaK"
                     /locus_tag="VBC81_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6912198.1"
                     /translation="MSKMIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            7973..9088
                     /gene="dnaJ"
                     /locus_tag="VBC81_001150"
     CDS             7973..9088
                     /gene="dnaJ"
                     /locus_tag="VBC81_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6912199.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9206..10453
                     /locus_tag="VBC81_001151"
     CDS             9206..10453
                     /locus_tag="VBC81_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6912200.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10524..11300
                     /gene="truA"
                     /locus_tag="VBC81_001152"
     CDS             10524..11300
                     /gene="truA"
                     /locus_tag="VBC81_001152"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6912201.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            11263..12021
                     /locus_tag="VBC81_001153"
     CDS             11263..12021
                     /locus_tag="VBC81_001153"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6912202.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12031..12495
                     /locus_tag="VBC81_001154"
     CDS             12031..12495
                     /locus_tag="VBC81_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6912203.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12492..13061
                     /locus_tag="VBC81_001155"
     CDS             12492..13061
                     /locus_tag="VBC81_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6912204.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13098..>13881)
                     /locus_tag="VBC81_001156"
     CDS             complement(13098..>13881)
                     /locus_tag="VBC81_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6912205.1"
                     /translation="FTKLISILLLIIAFVIVRQVINYLFEKTVNRSLAFSRQKVARQK
                     TLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAGIAGVAIGLGAQGFLSDVVNG
                     FFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFDGTLHFIPNRNITIVSNKSRS
                     NMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIVGEPTVFGPTTNANGQFVYRI
                     NIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSNNHSR"
BASE COUNT         4599 a         2102 c         2904 g         4276 t
ORIGIN      
        1 ttgttggctg tgtgctggca cattgagaaa ctttatatgg aataaacttt ctggaataaa
       61 tgagacattg acatcagaca ttgatgttgt attttttgat aaaaatattt cttatgaaga
      121 aacggtggta ttagaacaac aattaaagga taactatcca cagtacgatt gggaacttaa
      181 aaatgaattt tacatgaaca ctcattctcc taatacccca aagtatacta gctctaaaga
      241 tgctatttcg aaatttcctg aaaagtgtac tgcagtggga gaaaggttag atgatagaaa
      301 tcaactcgaa ctttacctcc cttatggtga agaggaaata ttaaatttca ttgttagtcc
      361 tacgccatat tttgaagagg atctattacg ttataatgtt tatttgaagc gagtggataa
      421 gaagaaatgg aataatattt ggcctcgttt aacaatcata aaaaaatgat aacgttactg
      481 gagttggctt aacagctttt aaatttttag actgacaaat agtatagacc tttaataagg
      541 tcgtttatcg ataatcatag tgtactgtgt tataatcgtt atagcataaa aacacgttgc
      601 agttggtagt ctgcaagcgt tcagtgaacg tcagtaacct tccctccagg tcgtccattg
      661 caatttaata tttaaaggag ggggctgtat gaaaatgaca gtctattttg atggtaattt
      721 ttggttgggt ttgattgagt acgatgatga tggtgattac aaagtttttc ggtatttttt
      781 tggaaaagaa cctaaagatg atgatgtttt taattttata aatcataaac ttaatgattt
      841 gataaaaaaa tacgagtttg ttaaaacgga tatttctcta aagcgtacga atgaacacaa
      901 aaaaagtcct aaaagaatgc aaagagaaat taatagagaa aaacgtaagc cagtagtatc
      961 tacaaaagcg caattggcga tgaaaactat tcatatgagt ataaaaaacg aacgtcaatt
     1021 aagtcagaaa tgtaagaaaa atgagttaag aaagcatcgg taccaattaa aacaagaaaa
     1081 acgttaccaa aagaaaaaag ggcattgatt gtgtcatcat ataaaaagct tgagagtatc
     1141 atctcaagct ttttatatgt taactaatat gagttactaa atagtctgat aatgctcaat
     1201 taatgtgttt tgggaaaaaa tcatttcctt aaaggacacc attgtcatgt tgttatcacc
     1261 ttactttttt tatacttagt atatgtttaa gaaaggaata agttgttttg agagattttg
     1321 aggagttgtt tgataaagta aagccaatcg taatgaagct gagaagaaat tattttgttc
     1381 aactttggga atatgatgat tggattcaag aaggacgtat tgtacttttt aggttgcttg
     1441 aagagcatcc gtatttattg gataatgaaa gcaaactatt catttacttt aaaacaaaat
     1501 tttcaaatta tttaaacgat gtattaagac accaagattg tcaaaagcga caatttaata
     1561 aaatgcctta tgaagaaatc agtgaagtta gtcattatgt taagagtaaa ggactagttt
     1621 tagatgacta tattgcttat cgtgatactt taacaaaagt ggaagaaact ttgtcagata
     1681 ttgataaaga aaaatttgaa aaactgatta atggtgaacg ttttgcgggg aagaaaaaat
     1741 tcatacgaaa tattcaacct tttttcaatg cttttaaagc ggattaatcg attttatttc
     1801 tgcagtagat agctgcagaa tttttttata gggaaagttc gctgatatat tcctgtgaaa
     1861 gcctttgttt tttgttataa tataataaaa tgcttgtgag gtgtcagtat gagtaaagtt
     1921 cgtttatata tcgctcgtca tggtaaaaca atgtttaata caattggtcg tgctcagggg
     1981 tggagtgata ctcctttaac tacttttgga gaactcggga ttaaagagct tggttgggga
     2041 ctaaaggctt caaatattag ttttaaagag gctttttcta gtgatagtgg acgtacttta
     2101 caaactatgg aaattattct cagggaagtg caacaagaaa atattcccta tacgagagat
     2161 aaacgaatcc gcgagtggtg ctttggtagt ttggatggag gatatgatgg cgaccttttt
     2221 aatggcgttc tgcctcgagt ttcaaacgga gatatgagcc atttgacaca cgaagaaatt
     2281 gctaatttga tttgtcaagt tgatacggct ggctgggcag aaccgtgggc aatattgagt
     2341 aaccgtattc tgtcaggctt cactgctata gctaaaaaaa tagaggatat tgggggaggt
     2401 aatgcgattg ttgtcagtca tgggatgact attgcgactt ttttatggtt aatcgaccat
     2461 agtacccctc gtagtttagg cttagataat ggtagtgtct cagtcgttga ttttgaagat
     2521 ggaacatttt ctattcaatc tattggggat atgtcctatc gagaaaaagg aagagaaatt
     2581 ttagaaaaaa cattgcaatg aaaaaaaata aaattatccg attcagttta gttggtgttc
     2641 tacttgcgat actatgcttt agtctttttg ctttattgaa gcctaacagt caacaatcat
     2701 catctcaaaa gttgaggaat gaggatataa aaaagacatc ctctcaaaaa agaaataaga
     2761 aattacgatt accagctgta tcatcaaaag attggaactt gattttggtc aatcgtgacc
     2821 ataaacatga agaattaagt ccagatgtgg tgcctgttga aaatatttat ttggataaac
     2881 gtattacgaa gcaagctact cagtttttag aggctgctag agcaattgat tcacgagaac
     2941 atttaatttc gggttatcgt agtgttgcct atcaggagaa gttgttcaat tcttatgtta
     3001 ctcaagagat gactagtaac cctaatttga cgaggggaca agcagaaaag ttggtaaaaa
     3061 cttactctca gcctgcaggt gctagtgaac accagactgg attagcgatg gatatgagta
     3121 ctgtagattc tttgaatgag agcgatccta gagtagtcag tcagttgaaa aagatagctc
     3181 cacaatatgg ttttgtctta cggtttccgg atggtaaaac agcagaaaca ggggtaggtt
     3241 atgaagattg gcattaccgc tatgttgggg tagagtctgc aaaatatatg gtcaaacatc
     3301 atttaacatt agaagaatac ataactttat taaaggagaa taaccaatga ggaaacgttt
     3361 ttccttgcta aattttattg ttgttacttt tattttcttt ttctttattc tttttccgct
     3421 tttaaaccat aagggaaaag tagatgctaa ttctaggcag agtgttacct acaccaaaga
     3481 agaatttata caaaaaattg tgccagatgc gcaagatcta ggaaagtcgt acggtattcg
     3541 tccttcattt attattgcac aggcggcttt ggattctgat ttcggagaga aaatattagc
     3601 taataagtat cataatctgt ttggtttgct tgcagaacca ggaacgccct caattacctt
     3661 aaatgatagt agtacaggaa aaaaacagga aaagcaattt actcattata aatcttggaa
     3721 gtattcaatg tatgattacc ttgctcatat aaaatctgga gcgacaggca aaaaagattc
     3781 atatactata atggtgtctg ttaaaaatcc aaaaacttta gtgcaaaaat tacaagatag
     3841 tggttttgat aatgacaaaa agtacgctaa aaaaatgacg gaaatcattg atttgtatga
     3901 tttaacaaga tatgataagt gatattagca ctctttgaat aagagtgcta atttttgatg
     3961 ttttttcctt gactatcttt taaaaaggag tataataaaa tcatagttag cagtcgatgt
     4021 gtaagagtgc taatttcaga aagaccgaga ggggtgatgt ttttgtgatt acacaacgtc
     4081 aaaatgatat tttgaattta atcgttgagt tatttacaca aactcatgaa ccagtagggt
     4141 ctaaagcttt acagagaacg attgattcta gtagcgccac tattcgtaat gatatggcaa
     4201 aactagagaa gttggggtta ttggaaaaag cacacacttc tagtggacgg atgccgagtc
     4261 cagctggttt taagtatttt gtagagcact cccttagact agatagtatt gatgagcaag
     4321 atatttatca tgttataaaa gcttttgact ttgaagcatt caaattagaa gacatgcttc
     4381 aaaaagctag tcatatttta tcggagatga cgggctatac atccgttatt ttagatgtgg
     4441 agccagctcg tcaacgtttg acagggtttg atgttgttca gcttagcaac catgatgctc
     4501 tagcagtcat gactttagat gaatctaagc cagtgacagt gcagtttgct attccaagaa
     4561 actttttgac acgtgacttg atagcattta aagctatcgt agaggagcgc ttgctggatg
     4621 gtagtgtcat ggatattcat tataagttga ggacagaaat tcctcagatc gttcagaagt
     4681 attttgtaac tacggataat gttttacaac tatttgatta tgttttttca gagctgtttt
     4741 tggaaacggt atttgtagct ggcaaggtaa attctttaac atattcagat ctatcaacgt
     4801 atcagttttt ggataatgag cagcaagtag caatttcttt gaggcaaggt ctgaaagaga
     4861 gtgagatggc ttctgtccaa gtggcagata gtcaagaagc cgcacttgca gatgtgtcag
     4921 ttttaactca caaatttttg attccttacc gtggatttgg actcctgagt ttgattggtc
     4981 ctattgatat ggattatcgt aggtctgtta gtttggtaaa cattatcgga aaggtattgg
     5041 ctgctaaatt aggtgattat tatcgatacc taaattctaa ccattatgaa gttcactgat
     5101 aatggcagta ttcaataagt tatttaaaag gaggcactca gtgtcagaag aaattaaaaa
     5161 agacgattta caggaagaag tggaagccac tgaaactgaa gagatagttg aggaagttat
     5221 tgaagaaatt cctgaaaaat cagagttaga actcgcaaac gagcgtgctg atgagtttga
     5281 aaataaatac ttgcgtgctc atgcagaaat gcagaatata cagcgtcgct ctagtgaaga
     5341 acgtcaacaa cttcaacgct atcgttctca ggatctggca aaagcaatct taccatcgct
     5401 tgacaatcta gaacgtgcct tggctgttga agggttgaca gatgatgtta aaaaaggttt
     5461 agaaatgact cgtgatagcc ttattcaagc actaaaggaa gaaggcgtag aagaagtaga
     5521 agtcgactca tttgaccaca actttcatat ggcagttcag accctcccag ccgatgatga
     5581 acatccagcc gatagcattg cagaagtctt tcaaaaggga tataaattgc acgagcgtct
     5641 gctaagacca gcgatggtag ttgtctataa ctaaggtaaa tgagttttcg tttttgtccg
     5701 taatgacagt aaactagata gcaagttaga agctattcag cttgctgatt aaactatagt
     5761 gattgcttag aattggaagt aaaataattc gagtgcttac taagataaat tgaaataaaa
     5821 agtaataaag tattataaaa taagaggtat taacatgtct aaaatgattg gtattgactt
     5881 aggtacaaca aactcagcag tagcagttct tgaagggact gaatcaaaaa tcattgctaa
     5941 cccagaaggc aatcgtacaa ctccttcagt agtatcattc aaaaatggtg agattatcgt
     6001 gggtgatgct gcaaaacgtc aagcggtaac aaatccagat actgttatct ctatcaaatc
     6061 aaagatggga acttctgaaa aagtttctgc aaatggtaaa gaatatactc ctcaagaaat
     6121 ttcagcaatg attcttcaat accttaaagg ttatgctgaa gactatcttg gagaaaaagt
     6181 agaaaaagca gttattactg ttccagctta cttcaacgat gcacaacgtc aggcaactaa
     6241 agacgctggt aaaattgcag gtcttgaagt agaacgtatc gttaacgaac caacagcagc
     6301 cgcacttgct tatggtatgg acaagactga caaggatgaa aaaatcttag tttttgacct
     6361 tggtggtggt acatttgacg tatcaatcct tgaattaggt gatggtgtct tcgacgttct
     6421 tgcaacagca ggtgataaca aacttggtgg tgacgacttt gaccagaaaa ttattgattt
     6481 cttggtagaa gaattcaaga aagaaaatgg tattgatctt tctcaagaca aaatggctct
     6541 tcaacgcttg aaagatgctg ctgaaaaagc taaaaaagac ctttcaggtg taactcaaac
     6601 tcaaatttca ttaccgttca tcactgctgg ttctgctggt cctcttcact tggagatgag
     6661 cttatcacgt gctaaatttg acgatctcac tcgtgacctt gttgaacgta cgaaaactcc
     6721 agttcgtcaa gctctttcag atgcaggctt gtcattgtca gaaattgatg aagttatcct
     6781 cgttggtgga tcaacacgta tcccagcagt tgttgaagct gtaaaagctg aaactggtaa
     6841 agaaccaaat aaatctgtta accctgatga agtggttgcc atgggtgctg ctatccaagg
     6901 tggtgttatc actggggatg tgaaagacgt tgtacttctt gacgtaacac cattgtcact
     6961 tggtattgaa acaatgggtg gtgtcttcac taaattgatc gaccgcaaca caactatccc
     7021 aacatctaaa tcacaagtct tctcaacagc agcagacaac caaccagccg ttgatatcca
     7081 tgttcttcaa ggtgaacgcc caatggcagc agataacaaa acactcggtc gcttccaatt
     7141 gactgatatc ccagctgcac ctcgtggaat cccacaaatt gaagtaacat ttgatatcga
     7201 taaaaatggt attgtatctg ttaaagctaa agatctcggt actcaaaaag aacaacacat
     7261 tgttatccaa tctaattcag gattaactga tgaagaaatt gataaaatga tgaaagatgc
     7321 tgaagcaaat gctgaagcag atgcaaaacg taaagaagaa gttgatctta aaaatgaagt
     7381 tgaccaagcc atctttgcaa cagaaaaaac tattaaagaa actgaaggta aaggctttga
     7441 tacagaacgc gatgcagcgc aatcagcact tgatgagttg aaaaaagctc aagaatcagg
     7501 taaccttgac gacatgaaag ctaaacttga agctcttaac gaaaaagcac aagctcttgc
     7561 agttaaactt tacgaacaag cagctgcagc acaacaagca gctcaagggg ctgaaggtgc
     7621 acaatcagct gattcatcaa gcaagggtga tgatgttgta gatggcgaat tcactgagaa
     7681 ataattatta atattgttca gattcatttg aatataagca tgaaaactat actagcatag
     7741 taaagttctt cgtgataggg gttgctcaat aatctagata agtttcagat tacataagct
     7801 aatttcgcta tcactaaata aaaacatatt aataataaat aggcggggcg cctcgctccg
     7861 tctgttttat taagtgtcat atatatgtta actatttaga gctgtaactg ggcaagaata
     7921 attgttaatc tcttcaagtg tagtatatga acaaaatata aaggattaga taatgaacaa
     7981 tacagaattt tatgatcgtc ttggcgtttc aaaagatgct tctcaggacg aaataaaaaa
     8041 agcttaccgt aggatgtcta agaaatacca tccagatatt aataaggaag cgggagcaga
     8101 ggaaaaatat aaagaggttc aagaagctta tgaaaccttg agtgatacac aaaaacgtgc
     8161 tgcctatgat caatatggcg ctgctggggc aaatggcggt tttggtggct tcgatggtgg
     8221 tggctttggt ggttttgaag acatcttctc aagctttttt ggtggaggag ggatgcgcaa
     8281 tcctaatgca ccacgtcaag gagatgacct acaatatcgt gttaatttaa gttttgaaga
     8341 agctatcttt ggagctgaaa aagaagtatc ttataatcgg gagtcatcat gtcatacttg
     8401 ttcaggaagt ggagctaagc caggtactag ccctgtgact tgtcaaaagt gtcatggttc
     8461 gggtgtgatt aatgttgata cacaaacacc tctaggaaca atgcgtcgcc aagtgacttg
     8521 tgatgtttgt cagggtagtg gtcaagaaat caaagagaaa tgtccaacat gtcatggaac
     8581 tggtcatgag aagaagacgc ataaagtatc agttaaaata cctgcaggag ttgaaactgg
     8641 gcaacaaatt cgtttaacag gacaaggaga agctggcttc aatggtggtc cttatggtga
     8701 tctctttgtt attattaacg ttctcccaag ccaacagttt gagcgtaatg gatcaacaat
     8761 ttattatact ttgaatattg gctttgtcca agctgctttg ggtgatacaa ttgatatacc
     8821 tactgtccat ggagcggtag aaatgtcaat tcctgctggt actcagacag gaaaaacgtt
     8881 ccgtcttcgc ggaaaaggtg cccctaaact tcgagggggt ggacaaggtg atcagcatgt
     8941 tactgttaac atcgtaactc ctactaaatt gaatgatgct caaaaagagg cattgcacgc
     9001 ttttgcagag gcaagtggag ataagatggt acatccgaag aagaaaggtt tctttgataa
     9061 agtcaaagat gctttagatg ttgattaaaa aataaaagtc tagaatcgaa agtattctag
     9121 acttttttgt agttattgct agagggattg cttgaaccta aattttacag tacaatgaat
     9181 ctaatattgt agggggacaa tttttatggt aaccaaagtt gaagagattc gctcatattt
     9241 gatagcttct atacaaaatg gtaagttggc tccaggagat cgcctaccat ctataagaca
     9301 gttagctaat caattttcct gtaacaaaga tacagtgcaa cgagttttga tggaattgcg
     9361 ttttgataat tatatctatg caaagcctag gtcaggctat tacgtctttg attctcatca
     9421 agaggaagtt gaagaagggg ttagtttacc aaactctgag attgcaaata tagcttatga
     9481 tgattttaga ttgtgtttga atgagaccct tattggtagg gaagattacc ttttcaatta
     9541 ttactatcgt caagaaggtc ttcttgattt aagtaaagca gtggctaaat taatggaaga
     9601 aacaggggtc tatgttcccc ttgatgatat tgttattacg gctggtactc aacaggcatt
     9661 atttattttg acacaggtta cctttccaaa tcgaaaatct cgagttttaa tagaagaacc
     9721 gacttatcct cgtatgattg aactaatcaa aacacaaaat ttaccctatg aaactattcc
     9781 tcgaggtact catggaattg attttcagcg cttagaggag attttccaga cacaatcaat
     9841 taagtttttt tatgttatac ctcgcatgca taatcctttg ggaacatcct ataatccggt
     9901 agagatgaaa agattaatag agatggcaga gaagtatgat gtttatattg tggaagatga
     9961 ctatatgtct gattttgcaa gtcagtcacc attacattat tatgatactc acgggcgtgt
    10021 tatttatcta aaatcttttt caaaggctat tttccctgct ttaagattag ctgcgatttg
    10081 tttaccacaa gctttaaaat caacatttat ggcttacaag aagttgatgg attatgatac
    10141 taatctgatt ttacaaaaag cattagcgct ttatattgaa aatggccttt atgctaagaa
    10201 tagtcaatat ttgaaatatc gttatcagaa agaccttgca aattcaaaat ctattttagc
    10261 tgatcaccct aatctacccc catatagttt acatcacgat agtgtattat ttgattgttc
    10321 gaaactcgat aacttcaaaa tattacggca atacggcgat actttggaaa attatttttg
    10381 tcaaaaatcg catcaatctc tcttacaagt aaaaaatgat tcctgcttaa agcagttctt
    10441 gggatcgttg tagtataatt gtagggggac agtttaaaat agatattata gttttcataa
    10501 atgaaaggtg gtaaaataaa cccatgacaa ggtataaggc acagatttct tatgatggtt
    10561 cagctttttc aggcttccaa agacaaccaa attgtaggac agttcaagaa gaaattgaga
    10621 gaactcttaa gcgcctaaat agcgggaacg atgtgataat ctatggtgct ggtcgcacag
    10681 atgctggtgt tcatgcttat ggacaagtta ttcattttga tttaccacag gcgcgtgatg
    10741 tagaaaaatt acgctttggt ttagatacac agtgtccaga cgatattgat attgttaaag
    10801 tagagcaagt gtctgatgat tttcattgtc gatatgataa acacattaaa acttatgagt
    10861 ttttagtaga tatcggtagg ccgaaaaatc ctatgatgag aaactatgca acacattacc
    10921 cttatcctgt cattattgag cttatgcaag aagctattaa agatttagta ggcacccatg
    10981 actttacagg ctttactgct tcaggtacga gtgtagaaaa taaagtacgt actatttttg
    11041 atgcgaaaat tcagtttgaa gcgtctaaaa atcttttaat ttttaccttt acaggaaatg
    11101 gttttttgta taaacaggtg cgtaatatgg ttggtaccct gctaaaaatt ggtaatggac
    11161 gcatgcctat tagccaaatc aaaacaattt tacaggctaa aaatcgggat ttagcaggtc
    11221 caactgcggc aggaaatggt ttatatttga aggagattat ttatgaagac gaggaatgtt
    11281 ttagcaattt caggaaatga tattttctct ggcggcggct tgcatgcaga tttagcaact
    11341 tacgttgtta ataaattaca tggttttgtg gcagtaacat gcttaacagc aatgtcagat
    11401 aaagggtttg aagtcattcc tatagaggca tctatactaa agcagcaact agaaagttta
    11461 aaagatgtag agtttgggtc tattaaacta ggattgcttc ccaatgtaga aacagcacaa
    11521 gttgttttgg aatttgtaaa atcaaaacaa gaatgtccag tagtcttaga cccagtctta
    11581 gtttgtaaag aaaatcatga tttagaagtt agccaattac gtgagcagct aattgcgttt
    11641 ttcccatatg ctgatgtgat tactcctaat ttagtagaga ctcaattatt gacgggtctt
    11701 tcaattgaaa acttagatca aatgaaaatt gctgctgaga aactttacga tatgggggct
    11761 aaacatgttg ttatcaaagg gggcaaccgt ttaaatgctg aagaggctac agacctttac
    11821 tatgatggtg aacgttttga aacctatgtt tttcctgtag tagatgccaa taatacaggt
    11881 gctggatgta catttgcgtc aagtattgcc agccaattag ctatgggtaa aaatgtagaa
    11941 gatgcagtca aaatgtctaa aggtttcgtc tatcaagcta ttaaagcatc ggataagtac
    12001 ggagttgtcc aacattttta ggagatttta atgaggaaag agaaaactag tcaattggtt
    12061 caattggcaa ttgtaacagc tatatctatt gtattgggga tgtttataag tattccgaca
    12121 ccaacaggtt ttttgacatt actagatgct ggaatattct ttgctgcctt ttactttggc
    12181 aaaaaagaag gggctgtggt aggtgcctta gctggttttt taattgattt attgaaaggt
    12241 tatcccaatt ggatgttctt tagtttattg attcatgggg cgcaagggta tttagcgggt
    12301 ttaccaggta gaagacgtct tttaggtctt attagtgcaa ctcttgttat ggtattaggt
    12361 tatgctatag ctagtggatt gatgtatggt tggggagctg ttttaccaga tattcctggc
    12421 aatattatgc aaaatatggt tggtatggtc gttggttttg ctcttaataa gtctctagaa
    12481 agagtgaaga aatgatacta gattataata agttaaagca ggaaactaaa gctattgtag
    12541 ttgatattat tgagcgtagt gctcttaaaa aaggtcaaat ttttgtacta ggtctatcat
    12601 ctagtgaggt ttctggtggt ttaattggaa aaaattctag ttctgaaatt ggtgaaatta
    12661 tcgttgaagt tatcctcaaa gagttacatt cacgcggcat ctatttagca gttcaaggtt
    12721 gtgaacatgt taatagggcg ttggttgttg aagcagaatt ggctgaacgt caacagttgg
    12781 aagtcgttaa tgttgttcct aatctacatg ctggcggtag tggtcaggtt gcagctttta
    12841 agttgatgac atcaccagtt gaggttgaag aaattgtggc acatgctgga attgatattg
    12901 gagatacttc tattggaatg catatcaaga gagtacaagt tcccttgatt ccaattagtc
    12961 gtgagttggg aggggcacac gttacagcac tagctagtcg tcctaaatta attggcggtg
    13021 ctagagcagg atatacgagc gatccaatcc gtaaatttta aaataaacct ctgctatgca
    13081 gaggtttatt gtttatctca acgagagtgg ttattggaga aattgtatgt tggtaagtca
    13141 ataccttctt caagaatagc tttttgataa agtttgtaaa attcagcata gatgtcaaac
    13201 tgagcaccgt tttgcgtgaa gatattaata cgataaacaa attgaccatt agcattggtt
    13261 gtaggaccaa aaacagtagg ctctccaacg atagcaggat gttttgaaac atactcttca
    13321 ttgatttttg ttacaatatc agaaatctga tcaaggtttg tatgaacaaa taaaggaata
    13381 tcaatttgag cacgcatatt gctacgagac ttattgctga caattgtaat attacgatta
    13441 ggaatgaagt gtaaggttcc atcgaaatca tgaatttgtg tggtgcgtat cccaacatta
    13501 gtaacagtgc ctgatacagc accaacatta ataatatcac caacatcaaa ttggttttct
    13561 agcagaataa aaaagccatt gacaacatct gataagaagc cttgagcacc aagtccaata
    13621 gcaaccccag caataccagc cccagctaat aaactagaaa ttggaacgcc caatatactt
    13681 aaaatccaat agaatagaaa gaaataaagg gtatagttta aaacattgtg agaaagttta
    13741 gctaatgttt tttgacgagc tactttttgt ctagaaaagg cgagtgagcg attaacagtc
    13801 ttttcgaaaa gataattgat gacttgccta acgataacga atgcaatgat taataataag
    13861 attgatatta gtttggtgaa g
//
LOCUS       DATRNX010000048        13828 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRNX010000048 DATRNX010000000
VERSION     DATRNX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            153..2717
                     /locus_tag="VBC81_001157"
     CDS             153..2717
                     /locus_tag="VBC81_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6912206.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQFADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            2840..3358
                     /locus_tag="VBC81_001158"
     CDS             2840..3358
                     /locus_tag="VBC81_001158"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6912207.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3476..4156
                     /locus_tag="VBC81_001159"
     CDS             3476..4156
                     /locus_tag="VBC81_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6912208.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4260..4943
                     /locus_tag="VBC81_001160"
     CDS             4260..4943
                     /locus_tag="VBC81_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6912209.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4933..5721
                     /locus_tag="VBC81_001161"
     CDS             4933..5721
                     /locus_tag="VBC81_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6912210.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5730..6833
                     /locus_tag="VBC81_001162"
     CDS             5730..6833
                     /locus_tag="VBC81_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6912211.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6892..7761
                     /gene="rfbA"
                     /locus_tag="VBC81_001163"
     CDS             6892..7761
                     /gene="rfbA"
                     /locus_tag="VBC81_001163"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6912212.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7761..8354
                     /locus_tag="VBC81_001164"
     CDS             7761..8354
                     /locus_tag="VBC81_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6912213.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            8561..9607
                     /gene="rfbB"
                     /locus_tag="VBC81_001165"
     CDS             8561..9607
                     /gene="rfbB"
                     /locus_tag="VBC81_001165"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6912214.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9659..12877)
                     /gene="hylB"
                     /locus_tag="VBC81_001166"
     CDS             complement(9659..12877)
                     /gene="hylB"
                     /locus_tag="VBC81_001166"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6912215.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            13094..13576
                     /locus_tag="VBC81_001167"
     CDS             13094..13576
                     /locus_tag="VBC81_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6912216.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            13566..>13828
                     /locus_tag="VBC81_001168"
     CDS             13566..>13828
                     /locus_tag="VBC81_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6912217.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRH"
BASE COUNT         4473 a         2180 c         2801 g         4374 t
ORIGIN      
        1 ataatatact aaagaagcca ctttttaagt ggtttttttg gtatataaat aagttatttc
       61 aataataata ttgcaaatat tattaaaata taataaaatt aagaaaataa tggaattttt
      121 gataaagaat attaaaggtg aggataaaat ctatgagacc tatcgttaga aaaaagatgt
      181 acaaaaaagg taaatttttg gtcattgcag gtatcgttac tattttaggt ggtagtgcaa
      241 ttcttggtca agatgtcaag gcagaacagg cagaagcagt aacttcaacg atctcagaga
      301 aaactgatag cagtcagact atttcggata tttccaagtt aacgttacct gttaattcct
      361 cggaggctat gaaaaatagt gcagagcctt taataaaaac aggctttgct acatctgttt
      421 catctaatcc tagagagata gcagctaccc cagtcaaaac ctttgatgct agttcaaaag
      481 ttgttgtaaa agctagtact gctgagcact cagcaaatca aacaaattct aatgttaatc
      541 aagtagctaa tgatagtgaa gttattactc aacaaaattc aacaaagcaa ttaccaacag
      601 tgacttactc tgctcatgtt caagatattg gatggcaaaa gtcggttgat aatgcaacag
      661 tttcaggaac agtaggacag gaaaagcagg ttgaggcaat aaaattgtct attaaagccc
      721 ctgaaggaat aacaggaaaa cttagctata aaacttatgt taaaggacaa ggttggcaac
      781 cttcagtaga gagtggtcaa gttagtggta ctgttggtca gagtagacct attgaagcac
      841 taagcattaa ccttactgat aatttacaaa aattatatga tgtctattat cgtgttcatg
      901 ttcaagatat cgggtggatg gcttgggcta aaaatggagc ttacgctggg actttgggaa
      961 tgtcaaagcg ccttgaagct tacaaagtaa agtttaccct taaaggacaa tctgttttaa
     1021 caccaactat acttaaggaa gagagacctg tcttaaatta tcaggttaag gtaggccaaa
     1081 atggttggca atctaataaa cttgaaggac aaatggcggg aactttagga gaatccaaag
     1141 ccctagatgg agtaaaattc actcttagca ccttgaagta tggggacatt ctctatcgta
     1201 ctcatgtaca agataaaggt tggggagaaa ttttaggtag tcaaacaagt aaggattatc
     1261 aaggaaaaag actagaagct attcaattgg acttaagtgg gaatttaaaa caaaattatg
     1321 atatttatta tcgcgctcac gtccaagata aaggttggat ggcttggcaa aaaaataata
     1381 gtgtagcagg tacagtcgga gaatcgaaac gtttagaggc tttggaagta aaactagtag
     1441 ctaaagcttc agattttagt tcccaaacaa atcattcatt cttagaaatg aagcatcctg
     1501 gattaagtta tcaaacttat cttcaaaaag atggttggaa accaacggtt ttggaaggcc
     1561 aattaggagg tagtattgga ctttcaaaat ctataaaagc catcaaactt aacttaggta
     1621 gtacagctct aggaaatata gaatatagaa cttttcttaa tggttcaggt tggcaaactg
     1681 ttgttaattc agggagagaa agtaatgtac caaatgaatc tcaacaagta gaagctattc
     1741 agatgcgatt gacaggtctg ctatcgaaaa cctatagtgt gatgtatcga gttcacatgc
     1801 aagattatgg ttggcaggat tggacttata acaatgacat cgctggtagt actaaccaag
     1861 gaaaacgaat tgaagcaatc gaaattaaat tagttgagac tgctaaaatg cctataactg
     1921 tccaaacgac ctataaggga actggaaact ataatgtacg agtgacaaat gttattagtc
     1981 ctgggaattt aaaaattgct atttggtcag ataaaaacaa tcaggatgac cttaagtggt
     2041 ataccgtcac acccaaaaat catgaagcta gattgacatt taatgttaca aatcatagag
     2101 acaatggtaa atactttatc catgtttatc aggaaaatgc ttctaatcaa aaacaattat
     2161 tggtaaaaac ttctctacaa gtcgcacatt caaattataa tactccttac tttagccaac
     2221 gtgatggaag gtgggcaagt cgaagatatg gtatggcaac tttaggagaa actggttgtg
     2281 taccaacttc tctagctatg attttgtctt ctttgaaagg agaaactgtt ttaccaacgc
     2341 aatttgcaga ttacctttac cataagacag ttgaatttaa tcgtggtgtt caaggaacaa
     2401 cttctagagg aatcttaaaa gcagccaaag aatggggtgt aacagcaact gcattaggaa
     2461 ctcaagctaa ccttgtacaa gcgttgaagg atggttatca tgtcctagcg gcagtacaaa
     2521 ataatgtttt tgttcttcat ggaagtcatg aaatcgttct caaaggatat aataacggac
     2581 tgacacatgt ctctgaccct tacacaccgt ccttgtctgg ttggtatcca atttcacaac
     2641 tttggaaaga acagagctat tatagtgagg atagaattga tattggagca ccttttgtca
     2701 aggtaacaga tgcctaattt ttaaaaccat atgcaaaact agcatatggt tttaaagttt
     2761 ccagtactat tttatttaat tttatgatat aatgaagagt aaaacatttt aggggtttcc
     2821 ccttagaaag acaagtgaaa tggatttaaa taattacatc gcttcaattg aaaactatcc
     2881 tcaagaagga attactttcc gtgatatctc cccattaatg gctgatggta aagcatacag
     2941 ctatgctgtt cgtgaaattg tacaatatgc tgctgataaa gatattgata tgattgttgg
     3001 accagaagct cgtggcttta ttgttggttg cccagttgct tatgctcttg gtattggttt
     3061 tgctccagtc cgtaaaccag gtaaattacc acgcgaagtt atttcagctg actatgaaaa
     3121 agaatatggt cttgatacat tgacaatgca cgcagatgct attaaaccag gtcaacgtgt
     3181 tttaattgtt gatgaccttt tagctactgg tggaacagta aaagcgacaa ttgaaatgat
     3241 tgaaaaactt ggtggcgttg tcgcaggctg tgctttccta gttgaacttg atggattaaa
     3301 tggtcgtaaa gctattgaag gttatgatac taaggtattg atgaactttc caggataagg
     3361 cctattcaag atagatgaat gataagggcg cctctaagtg ctatgataat agttgagagg
     3421 tgctttttgt tacacatttc ataattttag gacgatagag aggatttgaa aggttatgac
     3481 ttatttggaa caatatcagt cagggcaatt gactctacca agtgctttat tttttcactt
     3541 taaatctatt tttaagacag cagacgattt tttagtttgg caatttttct atttacaaaa
     3601 tacaactaat ttatccgatc taacaccgag tcgtattgcg acatcacttg ataaaacagt
     3661 ggcagatatc aatcgttcta tatcgaattt aacaagtcaa ggtttactag atgtcaaaac
     3721 aattgagttg aatcacgaaa tcgagattat ctttgataca agccctgtgt ttgctaaatt
     3781 agataaactt ttcgaagagg ataatcaagt aataatagac aataaaaccg ctgattcgaa
     3841 tcgtctgaaa gatttagtag gagacttcga acgtgaatta ggtcgcttat taagtccttt
     3901 tgaattagaa gatttacaaa agacactgca agaagatcag acagatcctg atattgttag
     3961 ggcagctctt agagaagcgg ttttcaatgg gaaaacaagt tggaattata tcaatgctat
     4021 ccttcgcaac tggcggcgtg aaggattgac gacacttcga caaattgaag agagaaaaca
     4081 agcacgtgaa gataaccaaa tgaaagattt agcgatttcg gatgatttta aaaatgcaat
     4141 gaatttatgg gaataagttt actaggagga gacagaatgg ttgatacatt aggtctgctc
     4201 cttttctttt gatgttaatg atgttatact tttaacagag aaaaactgaa ggaaatacaa
     4261 tggatttaca attatcaaaa cgattacaaa aagttgcaaa ctatgttcca aaaggagctc
     4321 ggttattaga tgtaggaagt gatcatgctt acttacctat ttttttgttg cagatgggat
     4381 actgtgattt tgccattgct ggtgaagtgg tgaatggacc gtatcagtct gcgttgaaaa
     4441 acgtttcaga acatggtctc acttcaaaga tagatgttcg cttagcaaat ggtttgtcag
     4501 cttttgaaga agcagataat attgatacga tagctatctg tggaatgggt ggtcgtttga
     4561 ttgctgatat tttgaataac gatatagaca aacttcagca cgttaaaaca ctagtcttac
     4621 aacctaataa tcgggaagat gatttaagaa aatggttagc tgcaaatgat tttgaaatag
     4681 tagcagaaga tatcttgact gaaaatgaca aatgctatga aattttagtt gttaagcatg
     4741 gtcatatgaa tttaactgct aaagaattgc gatttggtcc atttttatta tccaataaca
     4801 caacagtttt taaagaaaaa tggcaaaatg agcttaataa attaactttt gctttaaact
     4861 ctattcccaa tagtaaaatg gaagaacgtg ctattttgga agataaaata caggatataa
     4921 aggaggttct tgatgaaagc tagagaactt attgatgtat atgaaactta ttgtccacag
     4981 gaattatcta tggaaggaga tatttcaggt cttcaaattg gatctttaga taaagaaata
     5041 aagacagtca tggtagcttt agatgttcga gagactacag tagctgaagc aattgaacgt
     5101 caagtagatc ttcttattgt aaagcatgct cctatttttc gtccattaaa ggatttggta
     5161 gcaacaccgc aaaataagat ttatatagac cttcttaaat cagacattgc tgtttatgtg
     5221 agccatacta atatagatat cgttcctaat ggacttaacg actggttttg tgaattatta
     5281 gatattcaat atcccgatat attatctgag acaagtaatg gttatggtat tggtcgcatt
     5341 ggtgatgtta gaccccagag ttttgaattt tttgcatgga aaattaaaga tgtgtttggt
     5401 ttagatagcg ttcgtttagt ttcatatgat aaatctaatc ctgaaattca acgagttgct
     5461 atttgtgggg gtagtgggca aagtttctac aaagaagcta ttgcaaaagg agcagatgtt
     5521 tttgtaacgg gagatattta ttatcatact gcacaagaaa tgatcacaaa tggtttgctt
     5581 gcgattgacc caggccatca tattgaggtt ttatttgttt ctaaaatagc tactatgatt
     5641 gagcagtgga aacttgaaaa aggctgggat atttcagttt tagagagtaa agctccaaca
     5701 aatccctttt accatatgta ggagctatta tgaaaaagat agcaattatt ggtgcaggag
     5761 cagtcggagc gacactagcc tattacttat caaaagaaaa ggatattcag gttactgttt
     5821 ttgattatgg agttggacaa gcaactaagg ctgctgcggg gattattagc ccatggtttt
     5881 caaaacgtcg taataaagcc tggtatcgca tggcgaggtt gggggctgat ttttattcaa
     5941 agctagtcac ggatttacaa aaagatggtt ttgaaacaaa attttatcag caaactggtg
     6001 ttttcttatt aaaaaaggac gagagtcagt tagagtcatt gtttgcacta gcagataaac
     6061 gtcgactaga atctcctcta ataggtgatt tacaaatact aaataaaagt gaagcaaata
     6121 ctcactttcc tgagttagat ggctatgaac agttacttta tgcgagtgga ggagcgcgag
     6181 tagaaggggc ggacttaaca cgaatattgt tagaagcaag tggtgttaat gtcatcaagg
     6241 atgaagttca ctttacgata actgataatg gctttcgtgt tcaaggaatt gattttgata
     6301 aacttgtttt agcgagtgga gcctggctag ctaagatatt agatgagcac aactatcagg
     6361 tagatattcg ccctcaaaaa gggcaattgc gagactatta ttttagcaat attaatacag
     6421 gaaaatatcc tgttgtaatg ccagaaggcg agctagatat tatcccattt gataatggta
     6481 aagtctctgt aggtgcaagt cacgaaaatg atatggcttt tgatttaaac attgatttta
     6541 aagtcttaga caagtttgaa gagcaagcta ttggctattt tccgcaatta aaaaaagcag
     6601 atacgacttc tgaacgtgtt ggaattcgtg cctatacaag tgatttttct ccattttttg
     6661 gccctgtccc ttgtatggag ggtgcatatg ctgcaagtgg attaggatca actggcttaa
     6721 cagtgggccc attaattggt tatgaattat gtcaattaat cttaaataaa gaaaatcagc
     6781 taaatttaga ggattacgat attacgaaat acgtgaaaaa gattagtaga tgatagcgtt
     6841 taaaaaaagt gttataatat gtaggattaa tataaataag gagaccttac tatgaaaggt
     6901 attattttag cgggaggttc aggtactcgc ttgtacccat taactcgtgc tgcatcaaaa
     6961 caattgatgc caatttatga taaaccaatg atttattatc cattgtcagt tttgatgttg
     7021 gcaggaataa aagaaatact gattatttct actccacaag atttgcctcg ctttgaagat
     7081 atgcttggag atggttcaga actaggaatc tctctttctt atgctgaaca accaagccca
     7141 gatgggttag cacaggcctt cattatcggt gaagaattta ttggcgatga tcacgttgcc
     7201 ttagtattag gtgataatat ttaccatgga ccaggtttat ctgcaatgct acaacgtgca
     7261 gctagtaaag aaagtggagc aaccgtcttt ggctatcaag taaaagatcc agaacgtttt
     7321 ggtgttgtcg agtttgatac tgatatgaat gccatttcta ttgaagaaaa accagcgcaa
     7381 cctaagtcta attatgctgt cacaggtctt tatttctatg ataacgatgt ggttgaaata
     7441 gctaaaaata ttaagccaag tcctcgtgga gaacttgaaa ttactgatgt taacaaagcc
     7501 taccttgata gaggtgattt atcagttgag ttaatgggac gtggatttgc gtggttagac
     7561 actggaactc acgaaagtct tcttgaagca gcacaatata ttgaaacagt acaacgcatg
     7621 caaaacgttc aagttgctaa ccttgaggaa attgcttacc gcatgggcta catcactcgt
     7681 gaacaagtcc ttgagttggc acaaccactt aagaaaaatg agtatggtca atacctactt
     7741 cgtttgattg gagaagccta atgactgaac aattttttga taaagaatta acttgtcgcc
     7801 caattgaagc cattcctgga ttgttggaat tcgatattac cgttcgtgga gacaaccgtg
     7861 gatggttcaa agagaacttt caaaaagaaa aaatgatacc gcttggtttc ccagaaagct
     7921 tctttgaggc agacaaacta caaaataata tttcgtttaa caaaaaaaat actttgcgag
     7981 gtctccatgc agagccttgg gataaatatg tttcgatcgc tgatgaagga cgtgtgatcg
     8041 gtacttgggt tgacctccgt gaaggtgaca gttttggtaa cgtttaccaa acgattatcg
     8101 atgcctcaaa aggtattttt gtcccacgcg gcgttgctaa tggtttccaa gttctttcag
     8161 ataaagcagc ttatacttat ctcgttaacg attattgggc acttgaactc aaaccaaaat
     8221 atgctttcgt taactatgca gatccaaatc taggcattca gtgggaaaac cttgaagaag
     8281 cagaagtctc agaagcagat aagaatcacc cacttctcaa agatgtaaaa cctttgaaga
     8341 aggaagattt gtaattttca acttcttaaa aggcattaac cttagcaagt aaaaacgaat
     8401 tagcaatgtt gtttgacgca agggtgaaag gaaacatgca aaaggcctgt tccctcaggg
     8461 atttgaaata ttgatttctt aggcatgaca ttccatagaa ctacttctga cagaacagta
     8521 tccttcaaac agcaaaaaga aaaaattttt cggagaaaaa atgactgaat ttaaaaacat
     8581 tatcgtaact ggtggagctg gtttcatcgg atcaaacttt gtacactatg tttacaataa
     8641 ccacccagat gttcatgtaa ctgtccttga taaattgaca tatgcaggta accgtgctaa
     8701 cattgaagcc attcttggtg atcgtgttga gttagttgtt ggtgacatcg ctgacgctga
     8761 attggtagac aaattggctg ccaaagcgga tgctattgtt cactatgcgg ctgagagtca
     8821 caacgataac tcattggaag atccaagtcc atttatccat acaaacttta tcggaactta
     8881 cactttgctt gaagcagctc gtaaatacga tatccgtttc caccacgtgt caactgatga
     8941 agtttatgga gatcttccac ttcgtgaaga ccttccagga aatggtgaag gaccaggtga
     9001 aaaattcact gctgaaacaa aatacaatcc atcatcacct tactcatcaa ctaaggcagc
     9061 ttctgacctt atcgttaagg catgggtacg ttccttcggc gtaaaagcga ccatttcgaa
     9121 ctgttcaaat aactatggac cataccaaca catcgaaaaa ttcattccac gtcaaattac
     9181 aaatatcttg gcaggaatca aaccaaaact ttatggtgaa ggtaaaaacg tccgtgactg
     9241 gatccacaca aatgaccatt caacaggtgt ctgggctatt ttgactaagg gtcgtattgg
     9301 tgaaacatac cttattggtg ccgacggtga gaaaaacaac aaggaggttc ttgaacttat
     9361 ccttgagaaa atgggtcaac caaaagacgc ttacgatcac gtaactgacc gtgctggtca
     9421 tgatcttcgt tacgctatcg attctacaaa attgcgtgaa gaactcggct gggaaccaca
     9481 atttacaaac ttttcagaag gtttggaaga aactattaaa tggtatacag aaaatgagac
     9541 atggtggaaa gcagaaaaag atgctgtaga agccaagtat gctaaaactc aagaagtcat
     9601 taagtaattg aaccaaaaaa cctatcattt tgatgatagg ttttttatgt acggctagtt
     9661 agatagctaa ttggtctgtt tttgtcatgg tttgaggttg gtaatagaca ttttgatagt
     9721 cattaccaac tttttgtact aggtaaagtc ccgctttatt cattttgaat tgattgttaa
     9781 tgagactctc ttgattatcg tgtttgataa cagcccaagt ttgactgttt ttatcataga
     9841 taacttgttg ttttgaacta ttttctagta attctacttc tttgctgttg gctaatttat
     9901 caaaacttgt gcgatcaatg tttggaacca tcatgtaacc atagctatca cctttgttgt
     9961 catgcttttg gcttatagtg ataaaaggat tgctaacgat tgaggtattt ttagaagtac
    10021 gattaatgct gttccaagta cctgtttgct ctttgcgttc aatatcaata gtgctatttt
    10081 taaagaagat ataaccaata ttgcgaccag gttcttttga ttctaaaaag acactttttg
    10141 tatctgtaaa ttgttgagaa cttgcttgtt ttaaatcaat agtttttcca ttgacgtatg
    10201 tagtataagg tgttttagaa tcgtcttttc gttgatcaat tgttgtagaa acatttccaa
    10261 tgccgttagt attcttgatg ttgctaccta aaaagacaat cttatcattt aagataaccc
    10321 aacctttttg tgctgttaag gtgcgatccc agttagtaaa atccatagcg gcaagagcaa
    10381 agtgatcatt aagtttgacg gaaccaacaa agtcagatgt tcctgtaact tgaccggttt
    10441 tttctttagc gtctttgcta tgtttgctca tgaattcctt agtggtatct tcacgcttag
    10501 catctttttc agttgttcca gccattttat aaggattgac ggttggccaa aaatgattac
    10561 tataatgaga ttgatcacta ttataaatat agaacatacc atctccggta taccaaccac
    10621 gtgtattttc atcattcatt ccttcatagt tgagggtacg tttagaatgt aatgaaagcg
    10681 cgaaaccaaa gtctttcttg gcattataat aagctaagcg gtccatgcta ttaaaggttg
    10741 ataaattact ttttaaaggt ttagtagcga ctgtactgtc attaagcagc ttattcatgt
    10801 tggcaatatc ggaatacgat ttgaggttat tgaagacatt gtagaattta tttgaagtga
    10861 taatcgtttt aatagttgat ttgaggtcta agtttcgctc ttcattagac atgttagcca
    10921 atctgaggaa acctctgaga acttcaactg cagccgcatg cgaagaagct gcctctctac
    10981 taattgagcg tccacgactc atatccatta actcaccttt tacaattaaa ggtaaaaatg
    11041 attgattaat ccatttataa accatatcaa gttcttgatt agagatttta tagtcagttt
    11101 cttgaatgat aggcagcaat tgtgtcaaac catctatcag aacattacca taggcgccag
    11161 tataagcaac atttgtatgg tcgatgtaag aaccgtcagc atagaagcct tcagctttag
    11221 tagcagtagt aaaaagattt tttagagaat gagaagtttt ttcgataata gtattgtctt
    11281 tacgaagtaa accttcaatg attttaacgc gccccatatc tactagatta ccaccaaggg
    11341 ctttaaatgg attgacaagc gttttacgga aatattctgc atcaggaaca aagtgttcaa
    11401 ttgggtcggt ataagttttt atttcagcgt cagtgaagta gttattcatg agagataggg
    11461 tacctgtaat tgagcgagga acaccgattt caaaatccca ccaattggca gagccttcta
    11521 tatctttatt aacattgtag aagttttgat gaagccaagc cagactctct tttacagtac
    11581 gaatagcttt ttcatttttg taaatagtag agtgtggatt ggtgatttgt ttagctaaat
    11641 cttccaaacg acgataagta gcggttaact gtgctgaatt attgagatta tctaaatctt
    11701 tccaaaggaa agtgcgatta gaatccagtt tgatagcttc gatgtttttg gcgttagttt
    11761 catctaattt ctgattaagc ttttgcatgt tcgaatcatt agtatcgtaa acataattac
    11821 caatagtcac gtcgttccat ttgtcgagga gttttgtaaa gttatcttct gttgaagctg
    11881 taactgataa cggtacttct ttaattattt tgccagattt atcagatatt ttaacagttg
    11941 ttgaaccagt agcgtttgga tacaatattc caccaacaat tttcccaaga gacggattgg
    12001 ttagagtgta ttggtagtca gctttattaa aaacgtaatt tttgtttaaa gccgtattaa
    12061 cgcttttttc aatttgtgtt gtgacgggtt gtggatgctc tgagtcttta gggccttttg
    12121 ctttcattga tatattatca aaagtaacaa aaccagttcc tttttcataa taaagttcaa
    12181 gtgtcacctc ggagacattt aactttgggt tatatatctt tgttaaggta tgtttgttag
    12241 tagtaccgct ggtcatctca gaaagccaaa gtcgcgtatt gttgtctttt ttctccataa
    12301 tacgaaggaa agcttgtcca gccttgttac ttgtttcaat atcaaactta acctcatatt
    12361 ttttagtggg atcaatgttc actttttgtg ttacagcacc tctaaagcct ttgtcgctgg
    12421 atagacttac ttggccttgt tcggtacgtt ggataatagg tgatttatca gcagtatttc
    12481 ctttatcaat ccaagcagac cattccctag cgcttgtatg tgaccagctt ccagatacag
    12541 ggttagtttg attaaaatct ccgtttttca ccagctcctt attttgagtt tgattatcta
    12601 ctacttgttt cattgtgcta tgagtggctg ttgcagttgc agttgaagct gtggggttag
    12661 acagatcttg ttgtgtggtt gtggtgctag tctccttgac agtggtactt tcagtctgga
    12721 gaacttcaat attattagtt tgagtatctc ttatttggga cgttgaggta gttttggtaa
    12781 gctcttcagc tttagcttgg taactattgt taaccaatat tgttcctaaa ataagggctg
    12841 aataaagcat aatacgatgt ttctttttga tttccatttt tactccttag ggtttaaaat
    12901 tgtaaaccgg tttttcctaa ttatagcatg ctttaaaggt ttatgataaa gaacctttaa
    12961 aatgtcatca aagtgaaaag taaataactg gtaaacaaaa taatttgttt aatcatttgt
    13021 gtgctacttt gatagtaaat tgcctaaata aaggctattt tttgttataa tatgagaatg
    13081 agaggagttt gaaatgacta aattagcaac aatttgttac attgacaatg gcaaggaatt
    13141 acttctattg catcgcaaca aaaaagagaa tgatgttcat gagggaaagt ggatctcggt
    13201 aggcggtaaa ctggaggcag gtgaaactcc ggatgaatgt gctaagcgtg aaattctaga
    13261 agaaacccat ttaacagtaa aaaaaatgga ttttaaagga gtcatcactt ttcctgaatt
    13321 tacgccaggt catgattggt atacctatgt ctttaaagta acagattatg aaggagagtt
    13381 aatttcagat gatgaatcac gagaaggaac cttagaatgg gtaccatatg atcaagttct
    13441 ctctaagcca acgtggcaag gagattacga aatttttaaa tggattttag aagatgttcc
    13501 tttcttttct gctaagtttg tttatgatga acaccaaaat ttaattgaaa agacggttaa
    13561 tttttatgaa aaatagagat tttagggtta gagaccagct cgaaacattc ggagtcagag
    13621 tttctgcttt aatcatagaa aatcaaaaac tgctattaat atatgctcct catctctata
    13681 aatactattt gccaggtggg gcgcttcaag ttggagaaga tagcaataag gctgttgcga
    13741 gagaagtact tgaggaaata ggtcttcaca gccaagttgg tgatttagcc tatattgttg
    13801 agaatcagtt taatattaag agacatca
//
LOCUS       DATRNX010000049        13702 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRNX010000049 DATRNX010000000
VERSION     DATRNX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(302..1546)
                     /locus_tag="VBC81_001169"
     CDS             complement(302..1546)
                     /locus_tag="VBC81_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO6912218.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
     gene            complement(1556..2629)
                     /gene="purK"
                     /locus_tag="VBC81_001170"
     CDS             complement(1556..2629)
                     /gene="purK"
                     /locus_tag="VBC81_001170"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6912219.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(2616..3104)
                     /gene="purE"
                     /locus_tag="VBC81_001171"
     CDS             complement(2616..3104)
                     /gene="purE"
                     /locus_tag="VBC81_001171"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6912220.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(3390..4652)
                     /gene="purD"
                     /locus_tag="VBC81_001172"
     CDS             complement(3390..4652)
                     /gene="purD"
                     /locus_tag="VBC81_001172"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6912221.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            4934..5737
                     /locus_tag="VBC81_001173"
     CDS             4934..5737
                     /locus_tag="VBC81_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6912222.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(5760..6737)
                     /locus_tag="VBC81_001174"
     CDS             complement(5760..6737)
                     /locus_tag="VBC81_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6912223.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(6745..7626)
                     /locus_tag="VBC81_001175"
     CDS             complement(6745..7626)
                     /locus_tag="VBC81_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6912224.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(7643..8560)
                     /locus_tag="VBC81_001176"
     CDS             complement(7643..8560)
                     /locus_tag="VBC81_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6912225.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(8557..9219)
                     /locus_tag="VBC81_001177"
     CDS             complement(8557..9219)
                     /locus_tag="VBC81_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6912226.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(9239..9682)
                     /locus_tag="VBC81_001178"
     CDS             complement(9239..9682)
                     /locus_tag="VBC81_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6912227.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(9695..10525)
                     /locus_tag="VBC81_001179"
     CDS             complement(9695..10525)
                     /locus_tag="VBC81_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6912228.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(10535..11422)
                     /locus_tag="VBC81_001180"
     CDS             complement(10535..11422)
                     /locus_tag="VBC81_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6912229.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(11510..12826)
                     /locus_tag="VBC81_001181"
     CDS             complement(11510..12826)
                     /locus_tag="VBC81_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6912230.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(12873..13571)
                     /locus_tag="VBC81_001182"
     CDS             complement(12873..13571)
                     /locus_tag="VBC81_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6912231.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
BASE COUNT         4160 a         2846 c         2297 g         4399 t
ORIGIN      
        1 tctagcaacg ttattggaga tgaaaaatta cttctactta ccattgagat agacgttatc
       61 tgaatactgc tattttttac tattattgca ttatctttga ttgctaatgt atctgtctct
      121 aatgtattag aatcttctct acttacactt tttagaatat cttgaatcaa ttcttatttc
      181 tccttttatt ttttgaaatt aatttttgga atgaaagatg cctacaaata agtcacaatt
      241 atcttttcaa caaagctaca actatattct gttatataca aatacagtct tttctatata
      301 attattttgt catcagactt tgtctatatt caaatgcttc tgctttttca gtcaactcga
      361 tagcaagttc tttatgctcc tctaacactg ctttgatgtc agaaaatggg actttaaaat
      421 actcttttcg cccattaact ttattaattt tatttttttc aaatctgtta tgtaactctt
      481 tttctaatga aaaagcttcc tcactaaaaa ttaaggcatg tacatcaaat tgaaatggaa
      541 cagatgcact ccctaattcg cgaatacgtt ccataggatc tagacgcctt gtaacaccaa
      601 tcttgtaaac atcttctcca aatgcaccaa tatttgaaat gatataaaca tatccagcag
      661 ttgcatgccc ttctcgataa tcaatatctt cttctaactc atctagttct gataatttat
      721 cttggtattc tgctaattga agatttaatt tttccttttc ttcctcatta gctgttttaa
      781 gtaattcttc aacgttatct aacatctgtt taaagtgttt tctatctttt tcaatttgtt
      841 ttctcttagc agctaattct gcttgaagtt tcttatcttc tctttccttc gcacgttgtt
      901 cacgaagaag ttctttttct tctaatttct gaagttcata ttctactgct aattgtaact
      961 ctgaaatttt tagctcaaga tagtcattag atagacatac caaatttctt gaatacatct
     1021 tattaagctg attaaatgat ttaacaactg catctacttt tttacttaga ttagtagccg
     1081 ttacctttac caggtgagca tcaacttctc cattaaaacc tcttatcatc gctttaataa
     1141 gttgattttg cattgctcgt ccttttgatt tactgttatc cagcaacata ggtcttacaa
     1201 taacacccgc ttttttatct tttactaact gtttttgcct ctttctcaga gtttccaatt
     1261 cttctttata cttagtacta tctgagaact tatattttct ctcaaaaaat ccgtactctt
     1321 gaagatctaa atcatcatta agaactatta cctctttttg taaagcttta agttctgatt
     1381 ggcgtttctt aagttcatca tcaagtttta aaatatcttt agcaaccttt atctgccttg
     1441 ttaaatcatc caaaatagat tgtttatctt ttaacttact ttctaaacta ctgattttag
     1501 cttccaaagc ctcatcttta ggctttccga atccaaatag acccatttat tccccctaaa
     1561 actcctccac ctcatccgca tcttccacaa acaccgttac atgtcccatt ttgcggttat
     1621 gtttcgcttc tagtttacca tacatgtgga ggtgagcgct agggttttgg gcaacatagt
     1681 caatcgcctg ctggacatgt tgtccaagga cattaagcat aacggctggg tcatggagct
     1741 tgattggtgg gagaggttgc cccagtacac ccaagatgtg ggtgtcaaac tgtgaaaagt
     1801 cacaggcttc gatagaatag tgccctgagt tgtgggggcg tggggcgatt tcattgacga
     1861 tgatatcatc tgcggtcaca aacatttcca cacagagggt tcctgatagc tggagttcct
     1921 tggcaatctg taccgccatt ttcttagcct tgtcagctag ttggtctgag atgcgagctg
     1981 gtacgatagt ttttgatagg atattgttgc ggtggatatt ttcctgaacg ggaaagaccg
     2041 tcacatcctt gccatttcca gacacgatga ctgagatttc aaggtcaaag tcgacaaact
     2101 cttccaagac acactcagct gagttggcta attgttgcgc ctctggcagg tcttctgctg
     2161 agcggataac cttttgccca tgaccgtcat aaccacctgt cgctgtcttg aggacatagg
     2221 tcttggtcaa gtcaagcccc tctaggtcaa ggctagacgt caccacctta tagggagcga
     2281 cagtcactcc agccttattt gccagaaagt ctttttcaaa gatacggttt tgagaaatgc
     2341 ggagcagatc agtcccctgc ggtagctgac cagctgacac aacggcatcc agaccatcgg
     2401 catcaacatt ttcaaactca taggtcaaaa catcgcaacg cgcagctaat tgtcccaaag
     2461 cctcaacgtc atcgtaaggc gccacaatca cctcgctaac gcgggaggca gggcagtcgc
     2521 tagcaggatc cagcgtaatg accttgtggc ccatgtagat agccgcaatc gccatcatct
     2581 gccccagctg accaccaccg ataatcccaa tggtcttaaa tgagttcatt gcttgactcc
     2641 tctgcgattt ttccttgttc ctcatggaaa tgagctagcg catcagccag attttggtcc
     2701 tcgatagaga gaatacggag ggctgttagg gcagcgttag tcgctcccgc ttctccgata
     2761 gccatggttg ccacaggcac accgccaggc atctgcacaa tggaatagag cgaatccaag
     2821 cctgaaaggg cacgtgattt gacaggcaca ccgataacag gcagggttgt tttagctgct
     2881 accataccag gcaagtgggc tgccccacct gctccagcaa tgatgatttt gataccacga
     2941 ccacgcgcct cctcagcatg cttgaacatg aggtctggcg tacggtgggc agagacgact
     3001 ttcttctcgt aagcaatgcc aaagttatct agaacttcag ccgttttttg catggttgtc
     3061 cagtcggatt tggaacccat gataatagaa ataattggtt gcataatatt aagatacaaa
     3121 gggacgtttc gacttgtcga aaattttcgt agaaaaatat gcggtaagtc cgaaatcttt
     3181 gatttcgaag acgcacccac gtcagggcga ctaggtgctt tagcagcaga cgcctacgac
     3241 gcaaaaatct gacgaagatg ctttagcatc taggaagatt tatctttttc actagaaaat
     3301 tagtcccgtg ttcagttcct ttcttctttc tttttggagc gaccttcggt cgctgattgc
     3361 tgacgctaat atttgaatga gtagtcttat tacttaatag ctttgcttcc gatgtcgttt
     3421 cggtagaaga ggcctgtggt gtcttgttgg gctagttggg tatagatttt gtcctgccct
     3481 gctttgacgc tgtcttctgt ggtgacaagc atatagactc gtccaccgtt tgagagcagt
     3541 gctttgccat tttccgcaaa cttagctccg gcatagtagg tgatgatgtc gccgtcggtc
     3601 ttttcaggca gtggcacacc tttctcgtaa tcgagcggat agccttctga ggcaacgaca
     3661 acgcccaaag tcacgccgtc cttctgccaa gtgatgtaag gctcgatgcc catcatgatg
     3721 tcgtcgatgt tctgagcaaa atcagaagtc aggcgaggga ggataatctg agtttctggg
     3781 tcaccgaaac gtgagttgaa ctcgataacc ttcggaccat cagccgtcag gataagcccc
     3841 gcatagagga cgcctagata aggacgccct tcagtaatca taccctcaag gataggctta
     3901 acgatggttt caaccgccgt atccactacg ctctgaggca ggtggggaac tggcgcatag
     3961 gcacccatac cgccagtgtt tggtcccata tcaccgtcat aggcacactt gtgatcctga
     4021 gctgtcggca tgatgtaaaa cttgtcgcca ttagcgaagg caaagaggga gaactcttca
     4081 ccatccaaaa attcctcgat aaccacacgc gctcctgagt cgccaaactt gttgtccaaa
     4141 agcatctctt gcgccgcctc taccgcctgc tcaacggttt cagccacgac cacgcccttg
     4201 cctaacgcca agccatcagc cttgaccacg attggtgcac cctgctcttc gatgtaggct
     4261 ttagcttttt caaaatctga atatgtgcca taggctgctg ttggaacgtt gtatttaacc
     4321 atgatttcct tagcaaagtc ttttgaccac tctagctccg ctgctgcctt tgttggccca
     4381 aaagctctaa gtccagcact gttgaaatca tcaacgatac cagccgctag cgcatcatca
     4441 ggaccaataa aagtccaagc aatctcattc tccttagcaa agtcaatcag cctagaatgt
     4501 tcggaaatta cgatatttac caagcctaga ccatccaagg tcataccatc attaccaggt
     4561 gccacaaaaa cctgatctac acccttagac actaacaact tcttagcaat cgcatgctca
     4621 cgaccaccag aaccaacaac aagcaatttc atatcagaat aactcctatt aaataatata
     4681 aaaagaaatt tgcaataaaa acgtattatt tataaaatta taacacattt gttcgctttt
     4741 aagttttttg ttgcatcttt tttgaaaaat taataacata atatatactt cacatatatt
     4801 atgttattaa tccttcatat tgctatttaa aacttatcat tgctgtttct tagaacttag
     4861 tttggcccct cgctaacttt atgttatact gaatatgtta agcgctttct taaagaggag
     4921 aggaccgtgc attatgacta tttatgacca aattgagtct gctcttgatt taatgactga
     4981 tttagaacat gaaattgctt gttattttat ggggcagccc atttcaaaag acgctcttgc
     5041 ttcttccatt gtcactaagc aactacacat ttcacaagca gccttaacgc gttttgctaa
     5101 aaaatgtggg tttaaaggct atcgtgaatt tgtttttgaa tatttaaaaa gccatgagac
     5161 catcagtcaa caattatacg gtcttcaaaa tgacaacact aagaaagtat ttatgaatta
     5221 tcaagagatg gtttctaaat ccgctgatat tattgatgaa gaacagcttc tagaagtatc
     5281 acatatgatt gagcaagctg atcgtgttta tttttatggc aaagggagct catcgctagt
     5341 agctaaagaa tttaaaatac gccttatgag gctgggagtg atttgtgagg ctctggatga
     5401 tacagatagt ttctcatgga ctaatagtat tgttaatgat agatgcctcg tcattgcctt
     5461 ctcactttct gggaacacca actctgttat cggtgcctta aaaatcgcaa gttgtcacgg
     5521 agctaaaacc gttctattta caaaacaacc tcacacaatt gactatgctt ttgacaagat
     5581 tattcaggtg gcctctgcta ggcatttaga ctacggcaat agaatttcgc ctcagatccc
     5641 aatgttaatc atggtagata ttatctatgc acaattttta gatattaata aaatagaaaa
     5701 agagcgaatt tttagagaaa ccattattca acgctaaatg aaaatcgagt agaaataatc
     5761 tactcgattt tttgctttgt aaattgactt cctgtcagtt gatcaaaaat atagtctttt
     5821 acttgtactg tcattggatc atgtccatag tctggtaata aaagatgctt tttagttgat
     5881 gtcaaacggt taaaaatggc aaattgactt gatggaggac aaatatcatc tttcaaggct
     5941 gttagtaaaa caacggggca agaaatccta tgtgcaaaat ttttaacatc aatataagcc
     6001 aaagttttta acacattatt ttctgtttta tgaaaaggat cactatattt aaaatagcgg
     6061 aagagttcgt cataaggctc actgacattc cctaaatcta atactcgtct aaagtctgac
     6121 aaaaatgggt aaacagcaac ggtcttgacg attttgggat taagagctgc agctatcaaa
     6181 gcaagtgctc caccttgaga ccagccataa ctataaagtt gattactatc aacactctct
     6241 aatgtagcaa tgatatcaat caattggaaa acatcaagat aaatgtcttt ataaaaaaga
     6301 tgatttggtc ctgagatcat tcctcgcaca atctggccct taactgtaat gccatcaaaa
     6361 tgccccttgt cttgagactg tccagcttga cctcgaacat ccattgaaac aacgccgtaa
     6421 cctgctgcta cataattaag ttggtcactc cagtcaggac tttggttttg gtaaccatga
     6481 aaataaaaaa ctacaggata gggtttattt gttttaggga ataaacattt ggcatagaca
     6541 atagagttat tgctgccttt aaaatgaaga tcataagctt gtgcaaaagt aatattgaaa
     6601 tcttttttgt ctagacgata ctcaatattc ccttgatatt tcattgtttg ttttttccag
     6661 aagtcatcaa aatcctcggg aatttggtct tgacctaggt attctctcat gtcatctagc
     6721 gacattgttt caatcatata cccgtcaccc cctgttttta aaatggtagt aagctcctag
     6781 cataccagct tggttttcgt gttgtgcaaa aactattatt gttttttcag caagacttgt
     6841 cacaagattt cgtttcaaag attcactaag tttatcttgt aaataatctt tttgagccat
     6901 gattccacca cctaaaacaa ctttttcagg atttaccaca taaaccatat tagcgattcc
     6961 ttgaccaagg tagtttatca tacggtctat acttgcaata catttctcat tgccttcttt
     7021 agcctcctga aaaatacgac gtccatccca tcggttaatt tcatctccat gcgctttggc
     7081 aacatcagca atcagtgcag ttgtagaggc taaatcttga aaatcaccat ctgaaaggtg
     7141 catatagcca acttcacaag cagaattact aaagccatga aaaactgttt tatcaataat
     7201 cagacaacca ccaatacctg ttccaatagt aagacaaagc gctattgaac tatctttggc
     7261 actacctgag acagcttccg ctaatcctgc acaattaaca tcattttcaa tttctgtgcg
     7321 tacttgataa gtgtcttcta aaactttctt aaattgtgtc ccagcatagt ttggaatttg
     7381 aggaccagag taaaatatgc atccttcatc tggatctacc atgccagcag atgagatagc
     7441 aattccatca atgctccctt ctgttagata gttatcgata atttgacaaa ctttttgtaa
     7501 aatgcctgga ccacctttgt aggcttctgt ggcaagctca cttgcctcaa caatacaacc
     7561 taaattatcc acaatgccat gcttaatcat tgtaccgcca atatcaattg ctactgttct
     7621 ggtcataatt atctacctca ctttaacaaa actgtttttt agcatgtcta atcatttctg
     7681 ctgcctcttt aataataggt ttatcttctt ctgttacaga tgctaaaggt aaccgaacgg
     7741 aacctatagt tagttgctca ttgatttcca atacagcttt gatcacagca tacatattac
     7801 catgacccga acaaagcttt gtgataatat cgttaattgt aaattgaagt tcacgcgctt
     7861 tttctaaatc tttatcaaca attaactgat ttaaagtcaa gtataattct ggcattacgc
     7921 cataggtacc accaatacct gcagctgctc ccatgagacg accacccaaa aattgttcat
     7981 ctggaccatt aaaaacaata tgattttctc ctccaatagc cacaaaattc tgaatgtctt
     8041 gaactggcat ggaagaattt ttaacaccaa ttacttgcgg attttgtaac atttttcgat
     8101 ataaatccga tgtcaaagca acccctgcca attgaggaat attgtaaata ataaaatcag
     8161 tttgtggtgc tgcttgacta atagtattcc aataatctgc tatggcatac tctggtaatc
     8221 gaaaataaat tggtggaatt gctgcaatag catcaacacc catagcttca gcatgcatag
     8281 ccaactcgac gctatcctta gtattattac atgctacatg cgctatcact gttagtttac
     8341 ccttagcaac ggacataaca ttttccagta caagtttacg atctgctaca ctttggtaaa
     8401 tacattctcc tgatgaacca ttaacataaa gtccttgaac acctttatca ataaaataat
     8461 tggtcagggc ttttactcgt tcgggacaaa tatcaccctt atcatcatag caagcataaa
     8521 atgcaggaat aattccttga tatttttgta aatctttcat tgataatccc cttgttgttt
     8581 gctgaaatag gcaaattgtt tttgtaaaat aataatttta aagtaagcaa aggcggtata
     8641 agcaaaaata aatatagctg acactgtaag cataaataag agactagact gcaccataat
     8701 aaaccaagtc atgattaata aaaacatcaa tacaccagtc catttcaagt ttaaacatgc
     8761 cattataaag ctctttttca cggtatctaa aagagacatt tcatagcggg cagccatagg
     8821 aaataggtat atgctcgtca tcacagtcaa taacaaaaag gcgtaacaaa ttatcttcaa
     8881 gaaaggcaca atgaaaccta tttgataatg aagaattgtt aaatcaaata tgatcacaac
     8941 caaaatactt agttcaacca aacctaaaag cataccttgg aaccactctt tttttaggtg
     9001 ttttatatac aatattaaaa gatttgtctg atcaccttct aatttttgcc acaacgttcg
     9061 atacaaacta attttagcag caccaatagt taccaatggt aggcaagtta tcataaagag
     9121 aaggtttagc aatgccagat caaaaacaac attgcaacct tgcataaagg ggttgttaag
     9181 atcaaaaatc gctgctatta attgatttgc ttttttcaca atatactccc tgtatttctt
     9241 acgccattaa cactttcaat accccttttt tgacataatt acctagactt cccataccat
     9301 ttggctgatg agcttcttct gggaaaaaga aggctatata attatctctc aaatcaaaag
     9361 aagtctcttc tgagcacttg acaaatccta tatcgcctaa gtattcctct acctctacct
     9421 tatcaccttt tcccaatttg ataacttcat atccttcaat gacataatgt atatcaaggt
     9481 attttttatg gtattcaaaa atatgatcaa atgactcact aagttgattt gattggacca
     9541 tgagtatcac ttctgggcta atatgatagg tgcctatatc taaattcctt aaatcatgac
     9601 ttagtagata atcaatcgct aaatctaaat taggattaat atctttataa tgcgttaaat
     9661 tcaataaatg atcgtaaatc ataataaatc ctacctatcc tttaacagct cccattgtaa
     9721 tcccctgagt aaaggatttt tggaaaacta agaatactgt cacaatagga acagcagcta
     9781 aggctgcacc tgccataatt aagccatagt ttgttgccat ttcagcctgc atagtagcta
     9841 ccccaagaga aatggtcaga ttatttctag aagttagcat aactaattgc ataaaatagt
     9901 cattccaagt attgataaat gtgaaaatag ctagggcagc aaatccaggt ttgacaattg
     9961 gaaatgctac attaataaat gttcgaatct ctccacaacc atcaatttta gctgattcca
    10021 gtaattctgt tggaatattt tcagaaaact gtttcatcaa gaagacccca aaaggccaac
    10081 caacaagagg taaaataacc gcccataagg tatcatgtat ccccataaaa ttgataattc
    10141 taactaatgg cactaaaaca acttgtttag gtaaagccat agcagcaata aaaagggaga
    10201 acaatatttt ttgaccgtaa aatcgtttct tagctaaaac atagcctgcc attgacgatg
    10261 tacagcaaac taggaacatt gtcataattg agataaagac actattccaa agccatctca
    10321 aagctgggtt ttgtacagtt aaagctttaa aattttctaa ggtcggtgct ttgggccacc
    10381 attgtggggg gatgataata gtatcaggtt gagatttaaa agctcctgtc ataatccaat
    10441 aaaatggaaa gataaaaagc actgtcaaaa gacaaaggat aagagccgtt aaaaagttat
    10501 aagcactgaa tgttttcttt ttcatgtttt cctcctaata ttccacatca tttcctaaga
    10561 tcttaaattg agcaaaactt attaaagcaa tcattactgc taggaatact cccatagtat
    10621 tagcatagcc atattctgat agtttaaagg ctttttcata aagataatac atcaaagtgc
    10681 ttgttgaata atttggtcca ccagatgtta ggagctggat taaggcaaaa cattgaaatg
    10741 aattaattgt tgttataact gcaatataga gtgtagttgg taagagactc ggccatttaa
    10801 tttgccaaaa aacttgcatt tcattggctc catcaacacg cgcagcttcg caaagagaat
    10861 tatcaatatt tcccatcgcc gcaatatata gaataatagg ttgaccaact gaggtcgtta
    10921 gcaaaataat aataatagca agtaatgccc agtgcttatc accaagccag ctaatattct
    10981 gttcaatgac atgacctgat ttcaagatgt aatttaaaat tccggacata gggtcataaa
    11041 tccatttcca aacaactgtg actgcaacac taccagttac tactggcaag aagaagacac
    11101 aacggtagaa agagcgtgaa aaaacatttt tttcataagt attggcagca acaaaaagtg
    11161 agaagaaaac cactactggc acagagccaa tcacaatgat tactgtattt atcaaggatt
    11221 tcatgaaaat agaatcatga aacatacgat tgtaattagc taacccaata aaagtgaact
    11281 gtgtcataga ataattaaaa aaactagtga caaagcccat aaccatcggt gcaaaaacaa
    11341 aaattaagaa aaataagaga ataggcgcta gaaaagcata agctatcatc gtttccctca
    11401 tctttaactt attagtacgc atacggtact ctcctttaca tcttaacttg actaaaaact
    11461 cctcttctat cccaatatgt gattgaccat agaaaaggag tctccttaat tatttagctg
    11521 cttttttaat ggtatcattt gctttttgag taaagtcttt caaagcatct gctggttttt
    11581 catcaccatt ggatacagat tgaaccattg ggaaccataa ggttctcatt tcagaaaatc
    11641 catcgatagt gttgtaatat ggtgaataat attgagtcca ttttgaaatc ttcatcatac
    11701 gtttatcacc tttataaaga tccccaaatg atgttctaac tgggaaagca cctgtacgta
    11761 taacatcttt tggtccccat tttttatcat cagcaataaa agtgataaat ttcttagagg
    11821 cttttacttt gttttcatct ttattattaa agaccgcaaa accattaaca aggtattcta
    11881 aatctggttt tccatcttct gatgggaatg gcacttcaag gtaatccact ttacttgact
    11941 ctaataattt tgcttgagtt tttggttgag ctggagccca taggatagtg aaagcagttt
    12001 gtccattggc gaagttttga atgtcagctg agccatcata ctgagaccca ttcatcaagt
    12061 agcctttctt aatccattca actatttttt tcattgattt tacagaattt ttagtgtcag
    12121 tggtatattt tgttacttct ttatctgtta ttggagcact ataaagatta gcaaaaaatg
    12181 cacgtggtcc ttgatctcct ccttgcccgt ttgcaaagaa tgaacctggt gtatagcctt
    12241 tattttttag tgcttttagt actttttcaa aatcactagt agtccaacct tcttttacaa
    12301 gtttcaaaac tcctgcatct ttaagcattt ttttattgaa cgccatataa aatggggcag
    12361 aacttattgg atacatgtag gctttatcgc cagacttaga agcttgaatg atgttcttat
    12421 tattgacatc cttaataaat tggtctgtaa ataaatcatt caaatctgct aatttaccat
    12481 ttttaccata ttgaataatt cgccctggtg catcaaaaag cacatcaggt gctgtccctg
    12541 cttcaattgc tgtagtgatt ttttcaggtc cagatgtgaa atcaattgtc tctagtttta
    12601 cttttatatt aggatttttc ttttcaaaag ccttgatgac ttttttctca taagtaccta
    12661 ctccatcctt agccttttct tgagtaaagg ttggaaaagc ccaccacgta atttcgactt
    12721 tatccttctc ggcagccata acagattgac tactgtctac acaagcaaac attgataatg
    12781 ctgcagctcc gaggcaaaaa ccaatcacac tttttttgat actcataact attccccttt
    12841 tttattttta ttttgatttt tgagtaacac ttttaactaa gtcctgagat gaaacgctcc
    12901 gctatttctt ttggtctagt gatagcaccc ccaactacaa ttcctgcaac acctatatga
    12961 ttaatttcat tagcctgctt aggagtatga attttacctt ccgcaatcac atctataccg
    13021 gcttgacaaa gcttattaag gagttctata tccggtcctt cttcttggcg gctgtaatct
    13081 gtgtatcccg atagagttgt acccacaaaa tcaactcctg cttcaaaagc atttttgcct
    13141 tcttcaaaag tacttatatc agccattaac aactgtttag gatatttcct ttttatcttt
    13201 tgaataaact cagctacact caaaccatca tgacgctctc taagtgtaca atctaaggct
    13261 attactgcaa tatctaaagt agctaattga tccacctctg tcatcgtagc agtgataaat
    13321 ggttcttgtg gaggatattc acgtttaata atgccgatga taggtaaatt agtaacttct
    13381 tgaatttcct taatgtcgcg gacactattg gctcttatac caaccgctcc tgcttcttga
    13441 gctgccaaag ctaaaagagg cataacacct ccactttcag tataaagagg ctccccaggc
    13501 aaagcttgac atgacacaat aatgccattt tttatttgct ttttaaaagc ttctttactt
    13561 agatgtggca tattctcact tccttatctt ttttaatttt agatatctac aaaccttatt
    13621 atatctattt ttaaagcgtt ttcaataatc tttacagact tagattttag ttttaacgtt
    13681 ttgatttagt cacggttaac gt
//
LOCUS       DATRNX010000050        13366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRNX010000050 DATRNX010000000
VERSION     DATRNX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(75..1412)
                     /gene="brnQ"
                     /locus_tag="VBC81_001183"
     CDS             complement(75..1412)
                     /gene="brnQ"
                     /locus_tag="VBC81_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6912232.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
     gene            complement(1608..3605)
                     /gene="metG"
                     /locus_tag="VBC81_001184"
     CDS             complement(1608..3605)
                     /gene="metG"
                     /locus_tag="VBC81_001184"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6912233.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3748..4623
                     /gene="tehB"
                     /locus_tag="VBC81_001185"
     CDS             3748..4623
                     /gene="tehB"
                     /locus_tag="VBC81_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6912234.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(4781..5476)
                     /locus_tag="VBC81_001186"
     CDS             complement(4781..5476)
                     /locus_tag="VBC81_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6912235.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            5812..7029
                     /locus_tag="VBC81_001187"
     CDS             5812..7029
                     /locus_tag="VBC81_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6912236.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            7013..7855
                     /locus_tag="VBC81_001188"
     CDS             7013..7855
                     /locus_tag="VBC81_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912237.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            7932..8759
                     /locus_tag="VBC81_001189"
     CDS             7932..8759
                     /locus_tag="VBC81_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6912238.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(8799..9155)
                     /locus_tag="VBC81_001190"
     CDS             complement(8799..9155)
                     /locus_tag="VBC81_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6912239.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(9157..9633)
                     /locus_tag="VBC81_001191"
     CDS             complement(9157..9633)
                     /locus_tag="VBC81_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6912240.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(9688..10838)
                     /locus_tag="VBC81_001192"
                     /pseudo
     CDS             complement(9688..10838)
                     /locus_tag="VBC81_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(10911..11459)
                     /locus_tag="VBC81_001193"
     CDS             complement(10911..11459)
                     /locus_tag="VBC81_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6912241.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(11527..12618)
                     /gene="serC"
                     /locus_tag="VBC81_001194"
     CDS             complement(11527..12618)
                     /gene="serC"
                     /locus_tag="VBC81_001194"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6912242.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            12751..>13366
                     /locus_tag="VBC81_001195"
     CDS             12751..>13366
                     /locus_tag="VBC81_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6912243.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAH"
BASE COUNT         4396 a         2551 c         2096 g         4323 t
ORIGIN      
        1 taaaaaaatc tgatataaaa aaggctaaaa ctaagtttta acctttttga catatctata
       61 aaattgaaat agtgctatcc ttcaaatttc tctaaatcaa atgcttggcc tttttgttta
      121 tctgacagca ccaaagaaaa tactatacca atcaacattg gaactagcca ccccattgaa
      181 atagtatgaa aggggaggaa tccaactaac tgagttaatg ttttttcttg ccattgtccc
      241 gctagaacct caacaaacga cactaaagtg acaagaccaa ttgtcaaaga cattcccttt
      301 tttgaaagcg gcaaccactt attaatcaaa ataattaaga caataacaat aacaatcgga
      361 tacaataaag ttagcacagg aactgaaaaa gtaattacag catttaaacc aagattagca
      421 attaaaaagc caatcaaagt aaaaacagta gcaaataatt tataattccc aaaacgaaag
      481 ttcttatcaa aaaattcaga tactgacaca ataagcccaa ctgttgttgt aaagcatgtc
      541 aatgttacca taatactcaa gaaataacga ccaaaatttc caaataattt ataagatgct
      601 tgtgataata cataagctcc tttattaaca tttgggtcag ctaaaatatc agctgggaca
      661 ggaaacttat tccctaaaaa acctaagcca atatataaaa tactaaatgc taaactagta
      721 acaattccaa caatccaaat ggttgataaa tattcttttt tagttttaaa accaaacttt
      781 ttaagcgttt ctgttgctac tagacaaaaa gcaactgctg caagtgcatc aagtgtatta
      841 tagcctgcta agacaccact tccaaacgct tttcctgcat agtcagcact cgccttagca
      901 ctctccaagt ttccataagc aatagctcca acaacaacca aagagagaat taaaaaggca
      961 aaaacaggcg ttaagatttt tccaacacta tctaatatac catttggtct aatagcgaga
     1021 taataagctg cagcaaaaaa acaggctgta aaacacaaaa gagcaatcgg actatgccct
     1081 acaattggag caaccccaat ttcaaaagaa accgttgctg ttctaggaat agcaaaaagt
     1141 ggaccaatag ttaggtagag tataactaaa aatgttaaag aaaaccacgg actaaatttt
     1201 tccgacatct ctgtcttata accaccatta gttagtgtgc ctagtaggag ggtaataata
     1261 gctaatccaa ctcctgacaa acaaaatcct aaaatcgctg gccagaaatc ttgtccagat
     1321 gctacaccta atgctggtgg aaaaatgagg ttccctgcac caaagaagat accaaataag
     1381 agtaatcccg ttaaaaagcc tttttttacc ataagcaacc attagtgtaa ataatctatt
     1441 tacacccttt ccataaattc tccttatcaa attctatcta aatgcttaga atcgatgtct
     1501 tttattatat caaaaataaa aataaattct aggtcatttt cataattttc caactattca
     1561 gtcacttttt tcttgaaaac aaaaaaagag cataatgctc ttttcgatta accaataatt
     1621 gatccatttg ccactgctga atcgaccgtc aacactgtca atttaccatc atgttctgct
     1681 gagagaatca tgccttgact gacatatttt ttcatcattt tacgtggttt gaggttagca
     1741 acaatttgga gttttttccc tactaactct tgttcatttg ggtaaaattt tgcaatccct
     1801 gataagattt gacgatggcc ctcatcacca gcatctagac ggaaacgaag aagtttatca
     1861 gaaccttcca ctttctcaac ttcaattact tctgcaacgc ggatttcaac agcgtcaaaa
     1921 tcctcaaact tgatttgacc tttgcttgat gtcaattcaa cttcttcagg aacccactcc
     1981 ttttccactg gtgcttttcc agcattcatt tgttctttga tataagtaat ttcgtcttcc
     2041 atatccaacc ttgggaaaat tggtgaacct tttgcaacaa ctcgaacacc ttctggtaga
     2101 tcagcaaaag tcaatttttc aagatcgaat gttgccccta aaccaagttg ttccataatg
     2161 gcatcggaag tttccatcat gaacggttga atgaggtgag caacaacacg tagactagca
     2221 actaaatgac tcattacagc tgctaactta tcacgatccg tttcgtcttt tgcaagtacc
     2281 cacggtgctg tttcatcaat atatttgtta gttcgtgaaa taagattcca tacagcttca
     2341 agggcacgtg ggaaatccac cgcttccatt tgtttatgat agttttcaat actatctgtc
     2401 gcaacagaag ctaaatcggc atcaaagtca gttgcaacag caaaccttgg tacttctcca
     2461 tcgaaatact tgttaaccat agcaatagtg cggttgagaa ggtttcctaa atcattagca
     2521 agttcatagt taatacgtcc aacataatct tctggcgtaa aagttccgtc tgaaccaaca
     2581 ggcaatgaac gcatgagata ataacggagt ggatcaagac caaaacgctc tactaacatt
     2641 tctggataaa cgacattccc ttttgattta gacattttac catcttgcat cacaaaccaa
     2701 ccatgagcaa ctagacgttt tggtaatggc aaatccagcg ccatcaacat gataggccaa
     2761 taaatagagt ggaaacgaag aatatcttta ccaatcatat gaatgtctgc tggccaaaat
     2821 ttatgatatt gactgagatc gtcagaccaa ccataaccaa gagcagaaat atagttcatc
     2881 aaagcatcaa tccagacata aataacatgt tttggatttg atggtacctg aactccccat
     2941 gtgtaagttg tacgagacac tgctaaatct tctaaacctg gttctataaa gtttttaagc
     3001 atttcattca tacgaccatc tggctgaata aattctggat gctcagcgta gtacgctttt
     3061 aaacgatctg catatttgct catgcggaag aaataagatt cttcagaaac tttttcaact
     3121 tcatgaccag atggcgcaac accaccaatc atattaccgt tttcgtcacg ataaacttct
     3181 gctaattggc tttcagtaaa aaattcttca tctgaaacag aataccatcc ggtatactca
     3241 tcaagataga tgtccccttg tgccaacaat tgttcaaaaa ttttagcaac cgcttcttca
     3301 tggtaagtat ctgttgtacg gatgaatttg tcataggaaa tatctaaaag ttcccataat
     3361 gttttgacac tttcagccat gccatcaaca tattcctgtg gtgtaatgcc tgcttcttca
     3421 gccttttgtt gaattttctg accatgctca tcaagaccag tcaaatactg aacatcaaag
     3481 cccatcatgc gtttgtaacg tgccaaaaca tcacatgcta tcgttgtata ggcagaaccg
     3541 atgtgcaatt tacctgatgg gtaataaatt ggggtagtaa tgtaaaatga ttttttttct
     3601 gtcatgattt ctcctataag ccaccctaat gagaggtgtt ttttatcatg atacattata
     3661 acataaaatc tttttcaact ggatatatct ctgaataata tatctacttt tgctataata
     3721 aaggtattaa aaatagagag ttaatatatg gttaatacaa aaacacttct cccctacaaa
     3781 actatgccgg tttggactgc acagtctatt ccaaaggcat ttttagaaaa gcataatact
     3841 aaggaaggca cctgggcaaa actaaccatt ctaagtggtt ctttagtatt ttaccagtta
     3901 tctcctgatg gagaggaaat ctcgcggcat atttttgatg ctagtagtga tattcctttt
     3961 gttgatccac aagtctggca taaagtttcg ccgaatagtc cagacttaag ttgctatcta
     4021 actttttact gccaaaaaga agattacttc cataaaaaat atggtctcac gcgcacacat
     4081 tctgaggtta tcgccagtgc acctctctta tctgagaaga gtaatatatt agaccttggg
     4141 tgtggtcaag ggcgaaactc actttattta tcgctgctgg gacatcaagt gacttctgtc
     4201 gattcaaacg gacagagcct tgtagcttta gaaaatatgg cattagaaga agagcttcct
     4261 tacaatataa aaaggtatga tattaatact gctgctattg aagggcacta tgattttatt
     4321 ttatcaactg tggtatttat gtttttaaat cccgactgca tttctgatat tattttacaa
     4381 atgcaatcac atactcagat tggaggatac aatcttatcg tttctgctat ggataccgct
     4441 gaaaatcctt gcccgcttcc attccctttt accttcaaag aaggacaatt gaaatcttat
     4501 tacaatgact gggaaatcat caaatataat gaaaatttag gtgaattgca cagggtggat
     4561 gaaaacggta atcgcttaaa attacagttt gcgactttat tagctcgaaa attaagatca
     4621 taaaagtgag tagaaatcta ctcactttta tctttggtta ggtaataagt atattctaaa
     4681 cccatacata attatcctat aaaatatata gagatgatat ttcaaatcat tattgtaaca
     4741 atcagcttta gtatggctct tcaacataaa acgccataca ttaggagtgc ctttctactt
     4801 gtaggttata aaattcatgg cctaacagca agttgacata taaaagagga attaatatta
     4861 atgataagct ccattcgccc agtttacttg aaaaataagt cgagcaaaca acaccaaaga
     4921 taaatcctcc aataataata atcgtatttc tagcaattaa gaaaatatca gaattttttt
     4981 cgtaaagtcc ctttgttaat aaataagcag cattcttgat attaccagtc atcatcatat
     5041 tagcataagg tgctcctctg agcgatttaa aggtatctac ttgtattgaa gcaaagaagg
     5101 ctagtcctgc cacagtaaac gacgatggta agaaaggagt caatataatt gttactatag
     5161 caacctgtgt caacgcaaaa ctagataata ggtaccagtg cagttgatgt ttattcgccc
     5221 aacgattcat gaaataagta aacgattgac ctaacatgaa aaccattata ggtagcaaaa
     5281 aagcaagcgc ttgactataa tgcttattac taagatgaat agcaaaagat aacaaattcc
     5341 ctgtttgtac acctgcaaaa cgttttcctt gtgttacata agtaaaagca ttgatataac
     5401 ctgaaataaa acagagtgca accgcacacc tgagcccttc atatatttga tattgacggt
     5461 gagacttttt agacatattt tcctctaact ttatggcaaa caaaaataag actgggcaat
     5521 cttgctcagc actttttttc taatagattg gtttaaaata gtattaatat tagcactagc
     5581 tagcctaaca gttctaaata aattattaag caatgataaa tgtagtttta gtatatcata
     5641 gcctcagcat tgaagcaatc tcactttatg aaaggataaa gaaaatctca taagtcattt
     5701 acccttaatt tgaagtttaa ttagttactt ttaattttac taccaaaaaa cttgcaccat
     5761 tcttaacatt tgttacaata aatacaacct attaggaggt cagtacagat tatgcacagc
     5821 actatttata aatgtcatca ttgccgttat ctaatttata aaagttttaa agatatcttt
     5881 ccttttgctc tcattttttc ttggattgaa atccttcaaa aactcgttat atcagatgat
     5941 tcttttttag ctcaaacaac aaagtccagt atttataaaa acgagtggtt atatacggtt
     6001 tccaatactc tttatgattt atctcattta ctaatctact ttatggctag ttattttgtg
     6061 gctagtttag tatggagagg cttatctaac caaactaaag aaagtttttc tgcaagttta
     6121 cttggctttg ccactgtttg gttgcttcta acttcagttg ttccaggtac agattatcac
     6181 tatcctagcc aacccatttg gcttatgctg gctttgattt cgtatctttt tatcttttta
     6241 actcgactgc ttcaaaatcg acatcatatt ttccagtata gtatgttagc tatactacta
     6301 ttaatctctt ctattttgtc acatatctta tatagttatc ctaatattga tttagaatac
     6361 cttatacaaa atcacttttc tcaatggctg ggtgatgggc cacatcaatt aattcctatt
     6421 atgctttggt ctatcttagc tatttttcta accttatcag gtctttttgt accagatatt
     6481 ttatttcgtc cttactccta tttttcagta gttagcgaaa atttaaatgc tgccttgagc
     6541 caacatacag ataaaatccc ttatctgtat accttttata ctgttaaaaa tagctttgct
     6601 atgtttggcg gtattggaat cctattatcc ttatttttag cagttttata tgaaagtaga
     6661 aaactacaat cgaagaatta ctataaattg acattactga ccttaactcc tcttattttt
     6721 gatcaaaatt taccattttt agtaggtcta cctgtcattt tacaaccaat attattcatt
     6781 ccgatggtat taacaactat ctttgcagag gcttttggag ctcttatgct ctacctaaaa
     6841 tttgtcgacc ctgcagttta tactgtccca agtggtacac ctagtctact atttggcttt
     6901 cttgcttcaa atggggattg gcgttacctt ccagtcactg caattattct agttgttggc
     6961 ttctttattt atcgtccgtt tgtcaaaatc gctttcgcta aggaggagca atatgaaaaa
     7021 attacttaga tggcttcctc ctgtactttt cattattatc cttataggaa tgactatctt
     7081 aggtaagtcc tatatcaata aagtaacagc tcacaaaata aaactctata actctcgaat
     7141 gactcctact attttaattc caggatccag tgctactcaa gaacgattta acagcatgtt
     7201 agcacagctc aaccaaatgg gagaaaaaca tagcgtttta aagttaactg tcaaaaaaga
     7261 caatagcatt atctacaatg gacaaattag cggcaatgac cacaaaccct acattgtcat
     7321 tggatttgaa aataatgaag atggttatag taacatcaaa aaacaaacaa aatggctaca
     7381 gattgctatg aatgatcttc agaagaaata taaatttaaa cgttttaacg ctatcggtca
     7441 ttcaaatggt ggcttatcat ggactatttt cctagaagat tattacgact ctgatgaatt
     7501 tgatatgaaa tcattgttaa caatgggaac accttttaac tttgaagaaa gtaacacctc
     7561 aaatcatact caaatgctta aagatttaat cagtaataaa ggaaatattc catcaagtct
     7621 catggtatac aatttggcag gaactaattc atatgatggt gataaaattg ttccatttgc
     7681 tagtgtggag actggtaaat atattttcca agaaaccgct aaacactata cccaactaac
     7741 agtaactggt aataatgcta cacattctga cttgcctgat aatcctgaag ttatccaata
     7801 tgtcgcagaa aaaattctta aaaatgagaa aggtaaatta ccaaaacctc actaaagctg
     7861 tagaatagca acgcctctgg tattttgata taatatattg cgaattaaac taaataaaaa
     7921 aggatattat tatgaaactc atttcatgga atattgattc cctcaatgcg gcattaacaa
     7981 gtgaatcaac acgtgcttta atgtcacgcc aagttattga tactttagtt gctgaagatg
     8041 ccgacatcat tgcgattcaa gaaactaaac tgtctgccaa aggacctact aaaaaacatt
     8101 tggaagtact tgaaacttac ttcccagaat atgatttggt ttggcgatca tcagtcgaac
     8161 ctgctcgtaa gggctatgct ggtacaatgt tcttatatcg taaagggtta aatcctattg
     8221 ttagcttccc tgaaatcgat gctcctacta caatggataa tgaaggtcgc attattactc
     8281 tcgaattgga aaactgctat atcacacagg tctatactcc aaatgcaggt gatggcctaa
     8341 agcgcttagc tgaccgtcaa atatgggata ttaaatatgc tgagtattta gcaacattgg
     8401 atagtcaaaa acctgttcta gcaacaggtg actataacgt agcccacaag gaaatagacc
     8461 tagctaatcc tagcagtaat cgtcgttctg ctggattcac agctgaagag cgccaaggct
     8521 ttacaaatct actagccaaa ggttttacag atacttttcg ttaccttcat ggcgatgttc
     8581 caaatgttta tagctggtgg gcacaacgaa gtcgtacatc caaaatcaac aatacagggt
     8641 ggagaattga ttattggcta acaagtaacc gtgtcgcaga taaaattacc aagtcagaaa
     8701 tgattcattc tggtgatcgt caagatcata caccaattat tttggagatt gaactataaa
     8761 ccaaaaaaag atgattgagt tataactcaa tcatcttttt atactaagtt gaggtcttta
     8821 tacttagtac ggtagccaat ctgaagaatt ttattatctt taactagcaa tggacgctta
     8881 ataagcattc catccgatgc caataagtcc aaagcttggt caagagttaa atcatcaaat
     8941 ttatctttta agcctaattc tctgtaagaa tttcctgaag tattgaaaaa ctttttaagg
     9001 tcataaggcg aattctctaa caattctttt aataacgaag ctttgggagg attgcttttt
     9061 atatcaatcg cttcaaaagt caagccgagt tcagttaact ctttttttgc tgaacgacaa
     9121 gtggtacatt tggggtattc ataaaacgta tacatattat ttttcctttt cgtattctaa
     9181 taaccaagat tttctttcta agccaccagc ataaccagtt aattgaccat atcttcctaa
     9241 aacacggtga caaggtataa ttagagaaat aggattttta cctattgctc ctccaacagc
     9301 ctgtgcagat tgacaattta gctctttcgc taattctcca taagttttgg tatctccaaa
     9361 agggattttc tgtaattcct gccatactct cgtctgaaat gctgtccctc tcaatgataa
     9421 tggaatacta ggtaaatttt gctgtgaaaa ataagcatct aaccacttct taccaagtaa
     9481 agtataggaa tttgacatat taacaatttc ctcttggttg actgctagca tatcatattt
     9541 ttgcccaaca aaataaagcc ctgataaacc tagattgtct gctagaaggc gaatttctcc
     9601 aagaggggac tgatagaatt cttgatataa catatgataa gcataactta aaaagccatg
     9661 aaaagcaatt catgactttt tagttcttta ttgttgtttt gcaattaaac gtacacggac
     9721 gatattctca tcaccttcaa attcttcaat taaagtagaa atcttgttat tatccgtttc
     9781 atctaaatct attagtgtgt aggcgtagtc accctttgaa cggttaataa tgttatcaat
     9841 atttatacct aacactgata cagcagttga aattttagcc ataatgttag gaacattctt
     9901 gttaattaaa gtaatacgaa atggtgcggt ttgaatttga tgtacattgg ggaaattaac
     9961 tgaattagtg atttctcctg tctccataaa acaacgaatg gtttgacttg ccataatagc
    10021 acaatttagc tctgcctcat ctgttgatcc gcctacatga ggaaagactg taattccttt
    10081 ttggtttaat aattctttgt ctccaaaatc agtaatatag cgcttgacaa caccagtttc
    10141 tatcgcttca aatagctctt ggttattaac taattctgca cgagcaaagt tgataatcgt
    10201 agttcctttt ttcatgattg aaaaagcttc cgcatcaaaa gtatgcttag tttcatttgt
    10261 taagggaaca tgaattgtga tatagtcaca agtttcaaaa atatccttaa tctctttaac
    10321 cctttgaaca tggcttgaaa tattccaagc tgtttcaatt gaaatatagg gatcataacc
    10381 aagaactgtc attcctaagc gtctagcaca ttcgcaattc tagctccaat ggcaccaagg
    10441 ccgataactc ccaatttttt tcctgcaatt tcattaccag caaaagcttt ctttcctgct
    10501 tcaatttgtt tgggaatatc tgttccagtt agagtattaa cccatcggtt agctcctaaa
    10561 taatcacgag ctgaaagtaa taaggcagca atgaccgctt cttttacagc attagcattt
    10621 gcacctgggg tattaaacac gactattccc tgtgcacttg cctcttcaat aggaatatta
    10681 tttgttcctg cacccgccct agcaatagct ttgaagttac ttgggaaatc ctgattatgc
    10741 aaattttgac tacgaataat ataggcatca ggattttctg acatatcccc atcaatctgg
    10801 aaacgattcc ctaactcttg taaaccaatt tgattaatat tattaaatgt tttgacacta
    10861 aggttatctc tttagtcatc attgaccaaa ataacccctt tctttttttc ttacatggga
    10921 tttctttcat caatctcacg atagatgctc gtttctccct tttcttttat cttctgataa
    10981 gccaatcgta ccccatctag aggtacttta ccacaatatt gatagcctaa tttatttaga
    11041 atatgctgca tagtgacatt tttttcatga gtatcgcaac gaaaatcagg tcctttatga
    11101 ccttcaatta acccttgaag gaatgtctgt gctagtcctc taccacgaaa ttgatttgag
    11161 atcgcaatac gatgaaatgt aagatagcga tggttatcat ggagccattt tccctcataa
    11221 atagcatcat aaacctcttc atgaccatca atgacagccg cataagtcgc tagcatccca
    11281 tcttcaatac caacccaagc ataaccgttc aagatatcat caataatatc gttacgatta
    11341 gggtatccat cttctttttg ccattgatca cttcctgttt tagcaatttc ggcacgcgct
    11401 tcctcaataa gaagcataat ttggtcaatt tcatttggaa aagctaatct gatttgcatt
    11461 atattaccca cttttatata ttatagacta taataacggt ttaattttct ttcgtgataa
    11521 catttactat gtattttctg cttcaaatgt tttcataaaa tcaattaatt cgatgacacc
    11581 ttgtttagga aaggcattat aaaggctagc tctcatacca cctaccgaac gatgtccttt
    11641 aatattttta aatccacgtt ctgtcgcttc ctttacaaat ttttcatcta aatctttatt
    11701 aatagttatg aaaggaatgt tacatagaga acgactcttt ttatctctga caggattact
    11761 ataaaattct gaggaatcaa tataatcata gagaagtcct gatttttcac ggttagcttt
    11821 ttccatagca tcaacaccac ctagactttt cacccattca aagactaatt tagcaatgta
    11881 aatactgtaa gcaggcggcg tattataaag agaaccagca tctgactgaa ccttataatc
    11941 caacattgat gataaagttg gttcctcatt aatcatatct tcacggataa taactaccgt
    12001 tacaccagca ggtccaatat ttttttgagc tcctgcataa atcattgcaa aatcttctac
    12061 tttatatttt acagctaata tattagaaga catatccgca ataacaggca ctccattagt
    12121 tttaggaata tcataaagcg atgttccttc aatggtatta tttgttgtca catgaacata
    12181 ggcagcttta ggatcaatct ctttttcatc aaactctgga atataatcat aaactgattc
    12241 ttctgatgaa gctaatagaa ttggttcaaa aggaattgtt tttgatagtt ttacagcctc
    12301 tgtataagct tttttccccc acgaaccagc aacatgatag taagctttac gccctctggt
    12361 aatattcaag ggaatcatac taaactgtaa ggatgcacca ccttgtaaaa agattacttt
    12421 ataattatcc ggaattccca tcaaatctct taagtaacgc tcagcacctt tgataatatc
    12481 atcaaactct ttggagcggt gcgatacctc caaaacactc atactagacc cttgataatt
    12541 aaggagttca gattgggctt ttacaagcac tggttttggt aagactgcag gacctgcaga
    12601 aaaattataa attatcatat aacacctcca tataattgtg taagattata gcacagtatt
    12661 ttgaatattg taaagatttt tttgattaat tttgcttttt attcgtgata taatggtaaa
    12721 ggcgaacaat caagtttaaa ggagtaattt atgattttga gggaattttg tgcagagaat
    12781 ttaacagact taaccagatt agataaagct attatttcac gtgtcgagct ttgtgataac
    12841 cttgctgtcg gaggcactac tcctagctac ggtgttatca aagaagctaa tcaatattta
    12901 catgaaaaag gtattagtgt cgctgttatg attcgtcccc gtggaggaaa ttttgtatac
    12961 aatgatttag aattacgtat tatggaagag gatatcctac gtgctgttga gttagaatca
    13021 gatgctcttg ttttgggaat attaacttca aataatcata ttgatactga agccattgaa
    13081 caacttctac ctgctaccca aggtttgcca cttgtttttc atatggcatt tgatgttatc
    13141 ccgaaatctg accaaaaaaa atcaatagat caattagttg ctttaggctt tacaaggatt
    13201 ctcctacatg gttcatctaa tggcgaacca attatagaaa atatcaaaca tatcaaagca
    13261 ttagttgaat atgctaataa tcgtattgaa attatggttg gcggcggtgt gactgctgaa
    13321 aactatcaat atatttgcca agaaactggg gttaaacaag cccatg
//
LOCUS       DATRNX010000051        12640 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRNX010000051 DATRNX010000000
VERSION     DATRNX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            31..3731
                     /locus_tag="VBC81_001196"
                     /pseudo
     CDS             31..3731
                     /locus_tag="VBC81_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            3941..4849
                     /locus_tag="VBC81_001197"
     CDS             3941..4849
                     /locus_tag="VBC81_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6912244.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            5403..6413
                     /gene="nrdF"
                     /locus_tag="VBC81_001198"
     CDS             5403..6413
                     /gene="nrdF"
                     /locus_tag="VBC81_001198"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6912245.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            6414..6827
                     /gene="nrdI"
                     /locus_tag="VBC81_001199"
     CDS             6414..6827
                     /gene="nrdI"
                     /locus_tag="VBC81_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6912246.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            6829..8994
                     /gene="nrdE"
                     /locus_tag="VBC81_001200"
     CDS             6829..8994
                     /gene="nrdE"
                     /locus_tag="VBC81_001200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6912247.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            9057..12224
                     /locus_tag="VBC81_001201"
     CDS             9057..12224
                     /locus_tag="VBC81_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6912248.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGYEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQMEKNLKPVDYKTPIFN
                     KALPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            12237..12626
                     /locus_tag="VBC81_001202"
     CDS             12237..12626
                     /locus_tag="VBC81_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6912249.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         4363 a         1964 c         2478 g         3835 t
ORIGIN      
        1 gcatactgat ttttggagga aataggaaaa gtggataaac atcactcaaa aaaggctatt
       61 ttaaagttaa cacttataac aactagtatt ttattaatgc atagcaatca agtgaatgca
      121 gaggagcaag aattaaaaaa ccaagagcaa tcacctgtaa ttgctaatgt tgctcaacag
      181 ccatcgccat cggtaactac taatattgtt gaaaaaacat ctgtaacagc tgcttctgct
      241 agtaatacag tgaaagaaat gggtgataca tctgtaaaaa atgacaaaac agaagatgaa
      301 ttattagaag agttatctaa aaaccttgat acgtctaatt tgggggctga tcttgaagaa
      361 gaatatccct ctaaaccaga gacaaccaac aataaagaaa gcaatgtagt aacaaatgct
      421 tcaactgcaa tagcacagaa agttccctca gcatatgaag aggtgaagtc agaaagcaag
      481 tcatcgcttg ctgttcttga tacatctaaa ataacaaaat tacaagccac aacccaaaga
      541 ggaaagggaa atgtagtagc tattattgat actggctttg atattaacca tgatattttt
      601 cgtttagata gcccaaaaga tgataagcac agctttaaaa ctaaggcaga atttgaggaa
      661 ttaaaagcaa aacataatat cacttatggg aaatgggtta acgataagat tgtttttgca
      721 cataactacg ccaacaatac agaaacggtg gctgatattg cagcagctat gaaagatggt
      781 tatgggtcag aagcaaagaa tattttgcat ggtacacacg ttgctggtat ttttgtaggt
      841 aatagtaaac gtccagcaat caatagtctt cttttagaag gtgcagcgcc aaatgctcaa
      901 gtcttattaa tgcgtattcc agataaaatt gattcggaca aatttggaga agcatatgct
      961 aaagcaatca tagacgctgt taatctagga gcaaaaacga ttaatatgag cctgggaaaa
     1021 acggctgatt ctttaattgc tctcaatgat aaagttaaat tagcacttaa attagcttct
     1081 gagaagggcg ttgcagttgt tgtggctgcc ggaaatgaag gtgcatttgg tatggattat
     1141 agcaaaccat tatcaactaa tcctgactac ggtacggtta atagtccagc tatttctgaa
     1201 gatactttga gtgttgctag ctatgaatca cttaaaacta tcagtgaggt cgttgaaaca
     1261 actattgaag gtaagttagt taagttgccg attgtgactt ctaaaccttt tgacaaaggt
     1321 aaggcctacg atgtggttta tgccaattat ggtgcaaaaa agattttgaa ggtaaggact
     1381 ttaaaggtaa gattgcatta attgagcgtg gtggtggact tgattttatg actaaaatca
     1441 ctcatgctac aaatgcaggt gttgttggta tcgttatttt taacgatcaa gaaaaacgtg
     1501 gaaattttct aattccttac cgtgaattac ctgtgggggt tattagtaaa gtagatggcg
     1561 agcgtataaa aaatacttca agtcagttaa catttaacca gagttttgaa gtagttgata
     1621 gccaaggtgg caatcgtatg ctggaacaat caagttgggg cgtgacagct gaaggagcaa
     1681 tcaagcctga tgtaacagct tctggctttg aaatttattc ttcaacctat aataatcaat
     1741 actaaacaat gtctggtaca agtatggctt caccacatgt tgcaggatta atgacaatgc
     1801 ttcaaagtca tttggctgag aaatataaag ggatgaattt agattctaaa aaattgctag
     1861 aattgtctaa aaacatcctc atgagctcag caacagcatt atatagtgaa gaggataagg
     1921 cgttttattc accacgtcag caaggtgcag gtgtagttga tgctgaaaaa gctatccaag
     1981 ctcaatatta tgttactgga aacgatggca aagttaaaat taatctcaaa cgagagggag
     2041 ataaatttga tatcacagtt acaattcata aacttgtaga aggtgtcaaa gaattgtatt
     2101 atcaagctaa tgtagcaaca gaacaagtaa ataaaggtaa atttgccctt aaaccacaag
     2161 ccttgctaga tactaattgg cagaaagtaa ttcttcgtga taaagaaaca caagttcgat
     2221 ttactattga tgctagtcaa tttagtcaga aattaaaaga acagatggca aatggttatt
     2281 tcttagaagg ttttgtacgt tttaaagaag ccaaggatag taatcaggag ttaatgagta
     2341 ttccttttgt aggatttaat ggtgattttg cgaacttaca agcacttgaa acaccgattt
     2401 ataagacgct ttctaaaggt agtttctact ataaaccaaa tgatacaact cataaagacc
     2461 aattggagta caatgaatca gctccttttg aaagcaacaa ctatactgcc ttgttaacac
     2521 aatcagcgtc ttggggctat gttgattatg tcaaaaatgg tggggagtta gaattagcac
     2581 cggagagtcc aaaaagaatt attttaggaa cttttgagaa taaggttgag gataaaacaa
     2641 ttcatctttt ggaaagagat gcagcgaata atccatattt tgccatttct ccaaataaag
     2701 atggaaatag ggacgaaatc actccccagg caactttctt aagaaatgtt aaggatattt
     2761 ctgctcaagt tctagatcaa aatggaaatg ttatttggca aagtaaggtt ttaccatctt
     2821 atcgtaaaaa tttccataat aatccaaagc aaagtgatgg tcattatcgt atggatgctc
     2881 ttcagtggag tggtttagat aaggatggca aagttgtagc agatggtttt tatacttatc
     2941 gcttacgtta cacaccagta gcagaaggag caaatagtca ggagtcagac tttaaagttc
     3001 aagtaagtac taagtcacca aatcttcctt cacgagctca gtttgatgaa actaatcgaa
     3061 cattaagctt agccatgcct aaggaaagta gttatgttcc tacatatcgt ttacaattag
     3121 ttttatctca tgttgtaaaa gatgaagaat atggggatga gacttcttac cattatttcc
     3181 atatagatca agaaggtaaa gtgacacttc ctaaaacggt taagatagga gagagtgagg
     3241 ttgcggtaga ccctaaggcc ttgacacttg ttgtggaaga taaagctggt aattttgcaa
     3301 cggtaaaatt gtctgacctc ttgaataagg cagtagtatc agagaaagaa aacgctatag
     3361 taatttctaa cagtttcaaa tattttgata acttgaagaa agaacctatg tttatttcta
     3421 aagaaggaaa agtagtaaac aagaatctag aagaaataac attagttaag cctcaaacta
     3481 cagttactac tcaatcattg tctaaagaaa taactaaatc aggaaatgag aaagtcctca
     3541 cttctacaaa caataatagt agcagagtag ctaagatcat atcacctaaa cataacgggg
     3601 attctgttaa ccatacctta cctagtacat cagatagagc aacgaatggt ctatttgttg
     3661 gcactttggc attgttatct agtttacttc tttacttgaa acccaaaaag actaaaaata
     3721 atagtaaata aaactaagta aactactcgg taatatgatg aaaagttcag tttaacatta
     3781 tgttaagctg gacttttagg gtattgactc ttatttttga ttaattttat attgattaac
     3841 ggcatataat attataatat tataattacg attagtatgt gatactgttc gctttgtaag
     3901 ctttcataag atgaactagc cttaaggagt ttggatagca atgaaaacga taaaagattg
     3961 gcaaataaat atttgcttag cgacctataa tggtcaaaaa taccttcgcc agcaattaga
     4021 tagcataata caacaaggtt atactgactg gatttgttta ataagggacg atggatctac
     4081 tgatgataca gtagctatta ttaaagaata tgttaataga gattcacgtt ttatatttat
     4141 taattctaac gatgatagaa agttaggaag tcaccgcagt ttttacgagt tggttaacta
     4201 taaaaaagca gatttttatg ttttctcaga ccaggatgat gtttggaaag aaaatcgttt
     4261 agaacgttac cttgaagaag cagagaaatt taaccaagaa ttaccattgc ttgtttattc
     4321 gaactggact tctgtagatg aaaaattaac agtattgaaa gaacataatc cagcaacagt
     4381 tattcaagaa caaattgcct ttaaccaaat aaatggtatg gttatcatga tgaatcatga
     4441 gttagcaaag ctttgggaat accgtcaaat tggcgcccat gattcttatg ttggaactct
     4501 ggcttacgcc gttggaaatg tggcttatat ttctgacagt acagtccttt ggcgtagaca
     4561 agtaggggca gagtcgctaa ataattacgg gagacaatat ggggtggcta cgttttggca
     4621 gatgataaat acttcttttg atcgtgcttc gcttattttt gctcaagtat ccgataaaat
     4681 gtccctggag cgtaaactct tctttagtcg ttttatagaa ttaaaaaatg ccaatctaat
     4741 aagaaggatt tatcttttat caaaattaaa acttcgtcgg aaatcattga aagaaacagt
     4801 tgctatgact atcctacttt taacaggtta cggtaaacca aaagcataat ttttttggtt
     4861 gaaggaaatc aagttgagtt gtagttaaaa atatagaaaa tatttatttt tcaaaaagtt
     4921 gttgacactt aacgaaatta gtgtataata atattaaaga acaaggtaga accttggctc
     4981 gcattaattt cgggttttgg aaaaggagta gcaatcgcta ctcctttttt gttttacaca
     5041 ggaaaagtat gaaggaaaag ccttttttct tataaaaatt aagcttgctt aattttttaa
     5101 tttttatatt ctttacatta tgatgaaaac acaagatata gtggtgatat atgttgaaag
     5161 acacaaaact ttgtgttagt atactgttta agcagatcct taataaaggt aaccgacaaa
     5221 aaagtttaac gtgacttaac aaaacagtgg ttttgaagca agagttagct tcttaccaga
     5281 ataaaaaaac atcaagaaga acattccaat agttgtgtga ttttaacaaa aatccagtcc
     5341 tcttgggagg cttttttggt gcttaaaact cgcttaattt taatatttag gagactagtt
     5401 taatgcaatc atactatgat cgttctcaat caccacttga ctatgccttg tcagaaaaag
     5461 cttttcctat gcgttctgtt aattggaata agctaaacga tgacaaagac ttagaggtat
     5521 ggaacagagt aactcaaaat ttctggttac ctgaaaagat cccagtttct aatgacctta
     5581 attcttggag aacattggat gctgattggc agcaattaat tactcggact tttactggtc
     5641 taaccttatt agattctgtg caggctactg ttggtgatat tgctcagatt aagcattctc
     5701 agacagatca tgaacaagtt atttatgcta actttgcctt tatggtagct attcatgcgc
     5761 gatcttatgg aacgattttt tcaaccttat gcactagcca acaaattgag gaagcacatg
     5821 aatgggtagt ggatactgaa agtctacaag ctcgatcacg tatacttatt cctttttata
     5881 ctggtgacga tcctttgaaa tctaaggttg ctgcagccat gatgccagga tttttgttat
     5941 atggtggctt ttaccttcct ttttaccttt cagccagagg aaaattacca aatacgtctg
     6001 atattattcg tttaatttta cgagataagg taattcataa ctattacagt ggctataagt
     6061 atcaacagaa agttgctaaa ttatctgtag aaaagcaagc cgaaatgaag acatttgtct
     6121 ttgacctcct ctatcagttg attgatttag aaaaagccta tctttacgaa ctatatgatg
     6181 gttttgattt ggctgaagat gcaatcagat ttagcattta caatgctggt aaatttttac
     6241 aaaatttagg atatgattct ccatttacag aagaagagac tcgtatatct cctgaggttt
     6301 ttgcacaact ttcggcgcgt gcagatgaaa atcatgattt cttctcagga aacggctctt
     6361 catatattat ggggattaca gaagaaacat tggatgaaga ttgggagttt taaatggata
     6421 gtacagtgaa ggtggtctat ttttcatcga aatctaacaa tacccatcgt tttgttcaaa
     6481 agttggcttg ttctaaccag cgtattccat cagatggtag tagtatattg gttactgagg
     6541 attatatttt gattgtgcct acttacgctg gaggaggaga tgataccaaa ggcgcagttc
     6601 caaaacaagt tgtacagttt ttaaatgttc gacaaaatcg ggaacattgc caaggggtta
     6661 tttcttctgg aaataccaat tttggtgaca cttatgccat tgctggacct attattgctc
     6721 gaaaattaaa tgttcctttg cttcatcaat ttgaattact gggaacacaa gaagatgtta
     6781 caagggtaaa ggagttactt tgccaattca ctagaaagga taaataagat gacacaatca
     6841 gatgcatatc tctcgttgaa cgcgaagaca cgctttagag atcgcacagg taattatcat
     6901 tttacttcgg ataaagaggc tgttgaacaa tatatgatag aacatgttga acctaatacg
     6961 atggtgttca catcactaat tgaaaagcta gattatttgg tttctaataa ctactatgaa
     7021 tcggaccttc taaaacaata taaccttgag tttatttgcc aaatttttga gcatgcatac
     7081 gctaagaaat ttgcttttct aaattttatg ggggctttaa aattttataa tgcttatgct
     7141 cttaaaacag aagacaatcg ttattatttg gaacactatg aagatcgtgt tgtaatgaat
     7201 gctctgtttt tagctgctgg tgatgaaaaa gctgcctatg acctagtaga tgacatgcta
     7261 gcaaatcgct ttcaaccagc aacacctacc tttttaaatg cagggaaaaa acgtcgtggt
     7321 gaatatatct cttgctattt gttgcgcatt gaagacaata tggaatctat ttctcgtgct
     7381 attagtacaa gccttcagtt atcaaaacga ggtggtgggg tagcactttg cttaaccaac
     7441 ttacgggaat ttggtgctcc aataaaaggt attaaaaatc aagctactgg tattgtacct
     7501 gtcatgaaat tactagaaga ttcgttttct tacgccaatc aattaggaca aagacaagga
     7561 gctggtgcag tttacttaca cgctcaccat cctgaagtat taacgttttt agatactaaa
     7621 cgtgagaatg ccgatgagaa aattcgaatt aaatctcttt ccctaggact tgttattcct
     7681 gatattactt ttgaattggc taaggcaaat aaagatatgg ccttgttttc accgtatgac
     7741 attgagcgag tatatggtaa accaatgtct gatatctcta tcacagaaga atatgaaact
     7801 ttattagcca atgcggatat tcgtaaaacc tttattagtg cacgaaaact atttcaaacg
     7861 atagcagagc ttcattttga atctggttat ccttatatct tatttgaaga tactgtaaat
     7921 gctaaaaacc ctcacaaaaa agagggacga attgttatgt ctaatttatg ttctgagatt
     7981 gcacaagtta atacagctag tcaattttca gaagacctga ctttcactaa ggttggacat
     8041 gatgtttgtt gtaatttagg ttctattaat attgcgcgtg ctatggatca agcggcggat
     8101 tttgaaaaat taattgctaa tagcattaga gcccttgatc gtgtatctcg aacttctgat
     8161 ttagatagtg ctccatctat taaaaaaggt aatgcagcta atcatgctgt tggtttaggt
     8221 gctatgaatt tgcatggatt tttggcgact aaccatattt attacgattc tcaagaggct
     8281 attgatttta cagattgctt cttttacgct atggcttatt atgcctttaa agcttctaac
     8341 catttagcaa aagaaaaagg aacctttgag ggcttctcgg aatcaagtta tgctgatggc
     8401 agttatttct atcaatatac cgaacaaaac tttgagccaa aaacacagcg tgttaaaaat
     8461 ctactagctg agtatggtct gactctacca agccaagagg attggagaaa gcttgtgcaa
     8521 agtattaaag agataggatt agctaatgcg catttattag ctgttgctcc gacagggtca
     8581 atcagttatc tttcttcttg tactccgagc cttcaaccgg ttgtatcacc tgtcgaagta
     8641 cgcaaggaag gagcactggg gagggtttat gtacctgctt ataagattga tgcagataat
     8701 tatgtctact acaaaaaagg agcttatgaa gtgggatctg aggcgattat caatattgca
     8761 gctgccgctc aaaaacacat tgatcaagct atttcgttaa cgcttttcat gacagatcaa
     8821 gcaactacgc gagatttaaa taaagcctat attcaagcat ttaaacaaaa atgtgcctct
     8881 atttattatg tacgagtgag acaggacatc ctagaaggta gcgagagtta tgatgatatg
     8941 ctggatgatt tcacttcatc ggacttagaa gactgtcaat cctgcatgat ttaactaaat
     9001 atactcaatc agaagattaa gagaaatata ttggctttta gaaaggcttt attgtaatga
     9061 caaaaaaaca tcttaaaacg cttgccttgg cacttactac agtatcagta gtgacataca
     9121 gccaggaggt atatggatta gaaagagagg aatcggtcaa acaagaacaa acccagtcag
     9181 cttcagaaga tgattggttc gaagaagata atgagaggaa aacaaatgtt tctaaagaga
     9241 attctactgt tgatgaaaca gttagtgatt tattttctga tggaaatagt aataactcta
     9301 gttctaaaac cgagtcagtg gtaagtgacc ctaaacaagt ccccaaagca aaaccagagg
     9361 ttacacaaga agcaagcaat tctagtaatg atgctagcaa agtagaagta ccaaaacagg
     9421 atacagcttc aaaaaaggaa actctagaaa catcaacttg ggaggcaaaa gatttcgtaa
     9481 ctagagggga tactttagta ggtttttcaa aatctggaat taataagtta tctcaaacat
     9541 cacacttggt tttaccaagt catgcagcag atggaactca attgacacaa gtagctagct
     9601 ttgcttttac tccagataaa aagacggcca ttgcagaata tacaagtagg ctaggagaaa
     9661 atgggaaacc gagtcgttta gatattgatc agaaggaaat tattgatgag ggagaaatat
     9721 ttaatgctta ccagttgact aagcttacta ttccaaatgg ttataagtct attggtcaag
     9781 atgcttttgt ggacaataag aatattgctg aggttaacct tcctgagagt ctcgagacta
     9841 tttcagacta tgcttttgct cacatgtctt taaaacaagt aaagttacca gataacctaa
     9901 aggtcattgg agaattagct ttttttgata atcagattgg tggtaagctt tacttgccac
     9961 gtcacttgat aaaattagca gaacgcgctt tcaaatctaa tcgtattcaa acagttgaat
    10021 ttttgggaag taagcttaag gttataggag aagcaagttt tcaagataat aatctgagga
    10081 atgttatgct tccggatgga cttgaaaaaa tagaatcaga agcttttaca ggaaatccag
    10141 gatatgaaca ttacaacaat caggttgtat tgcgcacaag gacaggccaa aatccacatc
    10201 aacttgcgac tgagaatact tacgtcaatc cggacaaatc attgtggcgt gcaacacctg
    10261 atatggatta taccaaatgg ttagaggaag attttaccta tcaaaaaaat agtgttacag
    10321 gtttttcaaa taaaggctta caaaaggtaa gacgtaataa aaacttagaa attccaaaac
    10381 aacacaatgg tattactatt actgaaattg gtgataacgc ttttcgcaat gttgattttc
    10441 aaagtaaaac tttacgtaaa tatgatttgg aagaaataaa gctcccctca actattcgga
    10501 aaataggtgc ttttgctttt caatctaata acttgaaatc ctttgaagca agtgaagatt
    10561 tagaagagat taaagaggga gcctttatga ataatcgtat tggaactcta gacttgaaag
    10621 acaaacttat caaaataggt gatgctgctt tccatattaa tcatatttat gccattgttc
    10681 ttccagaatc tgtacaagaa ataggacgtt cagcttttcg acaaaatggt gcgcttcacc
    10741 ttatgtttat cggaaataag gttaaaacaa ttggtgaaat ggctttttta tccaataaac
    10801 tggaaagtgt aaatctctct gagcaaaaac aattaaagac aattgaggtc caagcttttt
    10861 cggataatgc ccttagtgaa gtagtcttac cgccaaattt acagactatt cgtgaagagg
    10921 ctttcaaaag gaatcatttg aaagaagtga agggttcatc tacattatct cagattactt
    10981 ttaatgcttt tgatcaaaat gatggggaca aacgctttgg taagaaagtg gttgttagga
    11041 cacataataa ttctcatatg ttagcagatg gtgagcgttt tatcattgat ccagataagc
    11101 tatcttctac aatggtagac cttgaaaagg ttttaaaaat aatcgaaggt ttagattact
    11161 ctacattacg tcagactact caaactcagt ttagagaaat gactactgca ggtaaagcgt
    11221 tgttatcaaa atctaacctc cgacaaggag aaaaacaaaa attccttcaa gaagcacaat
    11281 ttttccttgg tcgcgttgat ttggataaag ccatagctaa agctgagaag gctttagtga
    11341 ccaagaaggc aacaaagaat ggtcatttgc ttgagaggag tattaacaaa gcggtattag
    11401 cttataataa tagtgctatt aaaaaagcta atgttaagcg cttggaaaaa gagttagact
    11461 tgctgacaga tttagtcgag ggaaaaggac cattagcgca agctacaatg gtacaaggag
    11521 tttatttatt aaagacgcct ttaccattgc cagaatatta tatcggattg aacgtttatt
    11581 ttgacaagtc tggaaaattg atttatgcac ttgatatgag tgatactatt ggcgagggac
    11641 aaaaagatgc atatggtaat cctatattaa atgttgacga ggataatgaa ggttatcata
    11701 ccttggcagt tgccacttta gctgattatg aaggtcttta tattaaagat attttaaata
    11761 gttcccttga taagattaaa gcaatacgcc agattccttt ggcaaaatat catagattag
    11821 gaattttcca agctatccga aatgcagcgg cagaagcaga ccgattgctt cctaagacac
    11881 ctaaggggta cctaaatgaa gtcccaaatt atcgtaaaaa acaaatggag aaaaatttaa
    11941 aaccagttga ttataaaacg ccgattttta ataaggcttt acctaatgaa aaggtagacg
    12001 gtgatagagc ggctaaaggt cataatataa atgcggagac taataattct gtagctgtaa
    12061 caccaataag gtccgagcag caattacata agtcacagtc tgatgtaaat ttacctcaaa
    12121 caagttctaa aaataatttt atatacgaga ttctaggata cgttagttta tgtttgcttt
    12181 tcctagtaac tgctgggaaa aaaggaaaac gagcaagaaa ataaggagaa attaatatga
    12241 ttacacaaga aatgaaagag attattaata gtcagttagc aatggtcgcg acggttgatg
    12301 ctaaaggtca acccaatatt ggcccaaaac gttctatgcg tctctgggat gataaaacgt
    12361 ttatttataa tgaaaataca gatggtcaaa ctcgtattaa cattgaagat aatgggaaaa
    12421 ttgaaattgc ctttgttgat cgtgaacgtc ttttaggtta caggtttgtg ggaactgctg
    12481 aaattcaaac agaaggtact tattacgaag cagcgaaaaa atgggcagaa ggacgcatgg
    12541 gggttccgaa agcagttggt attattcatg tcgagcgtat ttttaattta caatctggtg
    12601 ctaatgcagg taaagaaatc acttaatata aaaaagcctc
//
LOCUS       DATRNX010000052        12526 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRNX010000052 DATRNX010000000
VERSION     DATRNX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..483)
                     /locus_tag="VBC81_001203"
     CDS             complement(<1..483)
                     /locus_tag="VBC81_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6912250.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
     gene            complement(635..5959)
                     /locus_tag="VBC81_001204"
                     /pseudo
     CDS             complement(635..5959)
                     /locus_tag="VBC81_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="VBC81_001205"
     CDS             complement(6202..8193)
                     /gene="uvrB"
                     /locus_tag="VBC81_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6912251.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(8254..9174)
                     /locus_tag="VBC81_001206"
     CDS             complement(8254..9174)
                     /locus_tag="VBC81_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6912252.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFTCFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            9357..11540
                     /locus_tag="VBC81_001207"
     CDS             9357..11540
                     /locus_tag="VBC81_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6912253.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            11540..12280
                     /locus_tag="VBC81_001208"
     CDS             11540..12280
                     /locus_tag="VBC81_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6912254.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
BASE COUNT         3906 a         2380 c         2026 g         4214 t
ORIGIN      
        1 tcctagttct tttttgttta ataaaatttt tcttataaaa ccatcttcaa gtcgcttact
       61 ataaaacatc tctgagatgt catcttcgtc aaatactctc aaaaggttaa gcatatcata
      121 tgctagttca atcttatcaa gttgtgaacg ttcaatccat ataacatcac cttcatctga
      181 cgattgtaat tcacctgaaa aatcatatgt tttataaagg aataccaaat aacggatgcc
      241 ttcatctgtg taccaatgct tcataccaac taatttggga ttacttattg ttaaacctgt
      301 ctcctcgcga acttctcgaa cgagagcatc atacaagctt tctccttctt caatatggcc
      361 gcctggtaga gcagctcctg accaagaata acgtttagga tcacggattt gcatcacaat
      421 acgtccttta ttatcttcaa taagacacat attagttaaa ataacttttt gactgcgtga
      481 cattatttcc tctagtatat aaatttagtg atagagttac aataccattt atttatcaat
      541 ttttctacaa aataatagat taagttaaaa ctcccttaat aatttatcca aaggaatttt
      601 agcttaataa attagaaaac taatttaatc aaaatcaata tttctttgaa gcttttttaa
      661 ggcctagtaa accaagtaca attactatga gtgcaattcc tttagccagt aaattattat
      721 tgtcatcact tgtccttggc aattcatgtt ttgccgctct agacattgga atcttagttg
      781 ttttcttcgc ttccatttga ctatgaacaa atgggggtgt ctttgtactc cctttcatgc
      841 ctacagctat cacttgtggt ttatccattt gatgtttaga tatttcttta aattcaccat
      901 gaactgctgg atcaacctta tgccctttta acgactctgt ttcttttttt agttttgtgt
      961 cgtcagagct tttatgatca tttttcaaac gtctgtcaga gttgatttct tccattgttt
     1021 ttacaatttg tatacttgtt tcgaaatggt tatatgcacc atttttaaat atcaaaccct
     1081 ttatagtatg atttcccact ttattatcga ctatttctgt caataaaata gagctatcct
     1141 tatccaagtc gaaaaggtcc gtaattcctt ttggatctgt taaaaggaga gtttgttttt
     1201 tcaaatgtgg ttttagtttt gaatctactt taaggtttga agttgataat aatgcttcaa
     1261 actctctaac cacagaacga ccaagggggt cagatatttt tgccgtaaag ctcacttttt
     1321 tgtccatgag ttggactggc ttaatcaatt ccgcagtctg attgtgggct agggataata
     1381 aattatcgat agaggtaaga tcttgcattt ggaaagaggt tttgcgtaaa cttgctgata
     1441 attcagggaa gttgccgatt gttataattt tttcagtgac tttacctgat ttatcaactg
     1501 ctgatacttt gatgacgtaa cgcccttcga aaccttttaa ttggtaagag tcaatacttt
     1561 gtccttctgt gtagggcttg ccattaacag ttatcattaa atttttaagg tgattatctt
     1621 ctgctttagc tataaccact ttgacttttc cggataataa atctgcttca ttaatagtta
     1681 acttcggtgt atccacatca ttaactacct tataatgttc agaaacggta ttacctgatg
     1741 tatctaccgc tgaaatcgta acaatatctc ctgctgctgc ttttatctta tgagtaaatg
     1801 ctcatctatt actgataaat tcaccacttg aatatttaac atcgacgcta tcaccattaa
     1861 tagaaagacc ccaacctttt acattatcag aaacgtgccc tttgatttca atttctccat
     1921 tttgagcata gatttgacca gtgataaagt tatctttgtc agcagcttct tctatgttag
     1981 tcttgtccag ttctaatttt ggaggttgta catcaacggt aatatgatag ccacgactaa
     2041 ataccaattt atcattctta ttataaacct tcaaattcat gatgttattc ccagacataa
     2101 atttgaccca tgtggtttta aatgtgtcag agtcatcacc atacatataa atagggttgg
     2161 atactgtacg atatgggtcg gaatgttttg gatctttagc gttctcatga aggttaacaa
     2221 ctcttatttc tgcacgatag ccatctttta ataagagaga aacactatgc tccaagcttt
     2281 cattttcaac atttcgaatt ttatttacag ttcgaattga atataccgtt tctcgagtta
     2341 ataataaacc atcctcgata tcagaaatat tttcttcaaa atcaaaatct ttttcttcgt
     2401 taaggtcatc atcgttatca tcccagatat cgtcatcatc tgaactggtt gcttgagctt
     2461 tcatattgcg atgagacagt gactgtggtt gtaatgtatt atcttcctca ctagagtaag
     2521 atttctctga tacttcttct gattctgata aagcaatttc cttgtttgaa ttcccagcat
     2581 agtctgcaac ttttatatcc agatcagatt ctcttaacgt taactcatca atatgatagt
     2641 gattttcggc aattttttta actgtgatag gcttttgtgt ctctttatta cgaacaatta
     2701 cttctttaac acgatgatta tcatgggtta aaatatcaaa gccgttatta acttttttaa
     2761 gagaaaaagt tggtctttca ttatctatta aaattggaag gtattggact tgataaggat
     2821 ctgtaggatt tgaacgtgca cgtaacttaa agtaatataa gcctggcttt gcagctttaa
     2881 aatcaccagt cttaggatca taaaactttc catcccaaac tagcgatgat tctgtcgttc
     2941 tcaaagcatc ataatatgag tcttcttcct catttcttga tttgtaatat ttctccacga
     3001 aatgtttcac tgttatacga cgaagtattg gtgaatcgtc ttttgcttct gatacaatag
     3061 agaggtcaaa atctgatgct gagcgtaata aagaaaatcg aggtgctaca cgcacaactt
     3121 tatcgggagc tacacgcaca ttagacatag taaataggga tggattgacc tttttactac
     3181 ctatttcttg tccgatttca taaaatttgt tagttccgtg tccaaccaaa gttgcttgca
     3241 tcaaggtcgt taatttattt ttagaacgac tatcccacat aggtgcgtca aaaattggtt
     3301 ctaaatccca tgttcctgag aagcccatgt aaggagctga gatatcttgt ccatctttat
     3361 ctaaagtttt gaaatacaaa tacccttcag caaaatcttg tataggtttt tttatctcaa
     3421 gatctatatc aagctctatg gctgattgcg gtgctagtga gataacttta cccatttgta
     3481 aagttgctcc ctctattact ttagcgtgaa ctgcctttaa aacgacattt tcatacccct
     3541 gattagtcac tttatcaaca attttagttg ttaaaatcgc tcctggttca atactaaagt
     3601 tacgacgttt ttgtcctgta ttgaccaatt tcaaggtaaa tcttttatgg ttcttaatct
     3661 cttttataga gaatgatcct gttttatcac caaaaacaag aacatcgtta gctaaagctt
     3721 tttctaagtt aaccatacct gcaccttgac gtcgtggtga aatttctaaa gctttattta
     3781 gagtatcttt caaaggttga cttgtattca tcaagacaat ctttgtcaaa tcaacgtttg
     3841 tcaaaccagc taacattgga agttttaatg ccattatttc agctacttta ctttttacta
     3901 atgcactaat ggcagctact tgaggggtag ccattgaagt tccagacatg tacccatagg
     3961 taccgtcgtt aaaagtagaa taaatatctt ctcctggagc aactacatct ggcttcaaat
     4021 gcaaatctgg tgtcactccc caactagaaa agcctgaaat tgtaggattt ggacggcgaa
     4081 caaaggtttc tttaggtaaa aattctaatt tagcagtttt gtttttcttg atgtagtcaa
     4141 tcaactgatc accatctttt tttgatagac taatagccca tttaccttct ggtatatcat
     4201 agtgataatc aaacatgggg tgagtatcaa aattcccaga actataataa gcaggttcat
     4261 ttatcataat aaccccttta gcaattccga taaaacgttt tagcttatct ttaacgccct
     4321 ctcctccacg ctcaacgacg ataacttttc cagtataact tttaaggtcc tcttctgaga
     4381 tatctttttt caactggcct ttaccaacat agactagatc aaatgtttta tttttgagca
     4441 tttttaatat tgtttgactt ttatgtccca gttctgcata tcctaattta agatttccaa
     4501 ctttcaaaac atctaaaaga agagcactat tttgaactga accaactcca ataaccttat
     4561 caacagctgc aacagacacc atagcagcag tatcttttgt tcccaaagag ttgtcaacga
     4621 cattatcgta ggtagtagat gaagccgaat taccatagtt tcccattgct gctactacga
     4681 taacaccctt gtcagtcacc ttcttaattg tctcatacca aagttcacct ggacgtcctg
     4741 ttcccaaata tccaaagctt aaactaataa cgtctgcccc tcttttagcg gcatcttcca
     4801 ttgcatggaa gactgcatca gagccggtcc actctgcaga tggatcttct ctatttgttg
     4861 aaattttgta cactaataac tgtgcattag gggcaactcc tttaaaacca tcctttgcat
     4921 ttcccgctaa gatacctgct aaatgcatac catgagggcg ctgaacgtcg tctttcaatt
     4981 ctaaattatc gcctcgttga taattgtatg caaaaggaac tttatttgta taaggagcct
     5041 taatatcttt aatacgacgc tttgccatca catctggatc attctcatcc aatcgcatgg
     5101 caggatgatg gatatctaca ccactatcaa tagtcgcaat aagtagccct ctaccgtcaa
     5161 ctttactatt ttgtttagct tttaaacgtg cagctaagac atgtgtccat tggttagcag
     5221 taaacatttg aggttttaat acttgaactg cttcaatatg ttttatctct ttaatcttac
     5281 caattttttc aatttctttt tcatctactg ctaatgaata gccattaaaa aggctactat
     5341 aagttaactt aatacgagtg cccggaattg ctttaagggc ttcatctact ttttccttat
     5401 ttacttgatc aaatgttaca ataacgcgct tatacacttt agaatcagat gggggagttg
     5461 gcttcgtcga agcattattc acgacatttt tcatatccac ttcattagcc ttattaggct
     5521 tattagtgtt tttgttttcc tcacttgaag gttgtgatga agtatttaat aacttttcac
     5581 ttgattgtac ttgcttttta gcctgtaaag aggtttcatc tttttgattt ttcgtttgtt
     5641 cttgaacgac ttgtttaaca ccagcaactt tatgtgcagg ttgttcactc tttagtatat
     5701 ttgaatctac agatgattta ttctcaagct tttgtgagtt actcgaggct attgttggag
     5761 gggttactgc attactttca gtacgtgact gtttaacttc ggaaggcttg atagttgtgt
     5821 ttttttctaa aagctgacta gtattcgcac tagtatgagg gactactgtg tctgcatcta
     5881 ccactacaga ggtcgctacc gcatttacag ctaaaaacgc aatcagtaca ccggttgttt
     5941 ttaaagaaat tcttttcata attccactca tttttaagtt agattatttt atttgttaag
     6001 caagcttaat ttaagcactt ttgtatagag ttgtcaatat ctatttgcta attaatgagt
     6061 attttttaag aatttttatc ttatttagat tagttctttt taaccaaact aaaaagctaa
     6121 ctctacctgc tagagtcagc ttatttaata atctataacc ttctgctatc tcctaaagag
     6181 agaaccagat gagagataag tttaatcgat tgcttttagt tctaagataa catctcggat
     6241 ttgtgcagct agctcaaagt ccaataattc agctgcatct tgcatttgct tttgaagagc
     6301 tttaatttta gcttgccttt cttttttaga taaactactg taatcgacaa ctttttcagg
     6361 cttatcgcta tcattacttt tagtaatagc aattaaatca cggatttctt tttttattgt
     6421 ttgtggcaca attccatgct tttcattata atccatttgt aaacgacgac ggcgagcggt
     6481 ctcatccata gctctttgca tagaatctgt tattttatca gcatacataa taacatgacc
     6541 attactgtta cgtgctgcac gtccgattgt ttggataaga ccacgctcat tacgaaggaa
     6601 accttcctta tcagcatcta agattgcaac aagggaaact tcaggaacat ctattccttc
     6661 acgtaagaga ttaataccaa ttaaaacatc aaacacacca agtcgtagat cacgaataat
     6721 ctctgtacgt tctaaggttt taatatcgct gtgcatatac ttaactttaa ctcccatttc
     6781 tttgagataa tctgttaagt cttctgccat acgctttgtc aatgttgtaa taaaggtacg
     6841 ctcacctttt tcagtcctca aattgatttc accaagcaag tcatccatct gccccatact
     6901 tggtctaact tcaacttctg gatcaagaag tcctgttggc cggataattt gctcaacaac
     6961 tgtatctgtt tgctccattt catagtctcc tggcgttgca gatacataga caatttgatg
     7021 gacatggctt tcgaattctt cacgacgaag tggacggtta tctaaggcag atggtaaacg
     7081 aaaaccgtaa ttaaccaaca tttctttacg cgaacggtct ccattataca tccctttgat
     7141 ttgccccatt gtcatgtgac tctcatcaat cataatcaaa aaatcttctg ggaagaaatc
     7201 taacaaagta aaaggtggct caccttcact tctaccatcc atgtgacgtg agtagttctc
     7261 aacaccattt gtatatccca tttcacgaag catttcaata tcatattctg tcctttgtcg
     7321 aatacgttgc gcttcaatca atttcccttc tttttcaaaa agttctactt gattttccat
     7381 ttcagcttgt atttttgaaa tagcttcttc catatgttca tcatttgtca taaagtgtgt
     7441 tgctgggaaa atagctaaat gttccacttc tcctagcaca cgccctgtta acgattctat
     7501 ttctcttata cgatcaattt catcaccaaa aaattcaatt cgaaaggcat gctcatcacg
     7561 actcgcagga aaaaattcta caacatcgcc acgaacgcga aattttccac gttgaaaatc
     7621 aatatcgtta cgctcaaatt gaatatcaac gagattgttc aataactgat cacgtgaaat
     7681 ttcttgacct ggcctaaggc taacaacact gtctgcatac tctttaggag aacctaaacc
     7741 gtaaatacaa gaaacagatg ctacaacaat aacgtcattt ctttctaata acgaagaagt
     7801 cgctgagtga cgtaatttgt caatttcgtc attgactgag ctatcttttt caatataagt
     7861 atccgaagaa ggcacataag cttctggttg ataataatca taatatgaaa caaagtactc
     7921 tacggcattg tcaggaaaaa attctttaaa ctctccataa agttgacctg ctagggtttt
     7981 attatgtgca atcaccaagg ttggcttgtt aacttgggcg ataacttggc tcattgtgta
     8041 ggttttacca gtaccagtag caccttttaa aatctgagct ttttctcctc cttcaatatt
     8101 gtcaaccaac gtttcaatag cttgaggttg atctcctgaa ggactatatt tggaaactaa
     8161 tttaaatcgg ttagtatctt ttctatctat catttcttta cctcgattac tttattttaa
     8221 catagaaaaa agaaaaagac tgagctgcaa cactcaatct gtttcttcag tcttaattag
     8281 ccatagaagg tatataattg cagctaagag aacaaaaata gaaatcaaac ttccttctat
     8341 tccgaatgca ccacctgata tccagtccgg tgcgttttct ttaacggtaa aatgcaaaag
     8401 agatgctcct gctttctgac cacttactgc aataccatag agattacctt ggctaaaatt
     8461 ccaagctcca tgtaaggctg caacgctcca aatattacca cttttaatca tatacaagga
     8521 cattaaaact cccgaacata taatacttac aatagaaaga aaagtgacat gggcatttac
     8581 caagtgcaaa atgccaaata aagtggatga gacccctatg gcaacagcta aatgaaaacg
     8641 atgattaatt agaggaagaa gccaccctcg tgttagcaat tcttccgtac cgctttgaat
     8701 aagccaaaaa ggaaggagag ttacaacata gaaaatcgtt gccttagaaa catcatagct
     8761 atgaaagtca agtcctccca atatataagc agtgatgaca gaaattgaaa atagaataaa
     8821 acctatactc cagcctttta taagtgacca cagagttctt ttaaactttg tagaaaaacc
     8881 caattctgaa aaaggtcttt tttctacgaa ttttacccag ataagcaaaa ggaatgctga
     8941 aaaactaaag gttcctaatt ctccatagat attattaaat aaattaataa actcaaaaaa
     9001 agtataggaa gaaaacttat agagtacaga agacactaga acaattccca caccacctag
     9061 agttagaccg acgaaaaaaa ataggtatac cataaaacaa gtaaataaca ataatagcca
     9121 tgacggccag tttttataga cagcgaactg actatttaaa atccttttat tcataataat
     9181 cctaaatcct ttttgtgtga tgacactaga acaattatat cataatacta actaaagata
     9241 tatataaaat taacataaca tacatgttac gttttctaac atagaaatga cattttgttg
     9301 ctctatctta tttattttga tataataaaa cacatattta taaaaaggag atttacatga
     9361 agcacaagtt aaaagctttt acgcttgctt tactctcaat attctttgtg tttggtggaa
     9421 aggtcagtgc agagactgtg aatattgttt ctgatacagc atacgctcca ttcgaattta
     9481 aagattctga tcaaacttat aaaggaatcg atgttgacat cgttaacgaa gtcgctaagc
     9541 gtgctggctg gaatgttaac atgacgtatc caggttttga tgccgcagtt aacgctgttc
     9601 aatctggaca ggcagatgcg ctaatggccg gaactactgt tactgaagca cgtaaaaaag
     9661 tctttaattt ctcagatact tattacgata cttccgttat tctttatact aaaaataata
     9721 ataaagtcac aaactacaaa caactaaaag gaaaagtagt cggtgtaaaa aatggaacag
     9781 ctgctcaaag cttcttagaa gaaaataaat ctaaatacgg ctataaagtt aaaacatttg
     9841 atacaagcga cctaatgaat aacagccttg attctggttc tatttacgcc gctatggacg
     9901 atcaaccagt tgtgcaattt gcgataaatc aaggaaaagc ttacgccatt aacatggaag
     9961 gcgaagcagt tggtagcttt gcatttgctg tcaaaaaagg tagtggacac gataatctaa
    10021 ttaaagaatt taacacagct tttgcacaaa tgaaatcaga tggcacttat aatgacatca
    10081 tggataaatg gcttggaaaa gacgctacaa aaacaagcgg caaagcaaca ggtaatgcca
    10141 atgaaaaagc aactcctgta aagccaagtt ataaaattgt ttctgattct tcattcgcac
    10201 cattcgaata tcaaaacggt aaagggaaat atactggttt tgatatggaa ttaatcacga
    10261 aaattgctaa acagcaaggt tttaaacttg atatctcaaa tccaggtttt gatgccgctt
    10321 taaatgctgt ccaatctggg caagctgacg gtgttattgc aggagccaca atcacagaag
    10381 cacgccaaaa aatctttgat ttttctgatc cttattacac atctagcgtt atcttagcgg
    10441 ttaaaaaagg aagcaatgtc aaatcatacc aagatttaaa aggaaaaaca gttggtgcta
    10501 aaaatggtac tgcctcatat acttggttat cagaccacgc agataagtac aactatcatg
    10561 ttaaagcatt tgatgaagca tctacaatgt atgatagtat gaactcaggt tcaattgatg
    10621 ctctaatgga tgacgaagcc gttcttgctt acgctattaa tcaaggtcgt aaatttgaaa
    10681 cacctatcaa aggtgaaaaa tcaggcgata tcggatttgc agtgaaaaaa ggggcaaatc
    10741 cagaattaat taaaatgttt aacaacggtc ttgcttcact caaaaaatcg ggtgagtacg
    10801 ataaacttgt taaaaaatac ctttccacag ccagcacttc ttcaaacgat aaagctgcta
    10861 aacctgtaga tgaatcaact attttagggt taatttctaa taactacaaa caattgctat
    10921 ctggtattgg aactacttta agtttaactc ttatctcgtt tgcgattgct atggttattg
    10981 gtattatctt tggtatgatg agcgtatcac caagtaatac tctccgcaca atttcaatga
    11041 tttttgttga tattgtccgt ggtattccac tcatgattgt ggccgctttt attttctggg
    11101 gtattcctaa tttaatcgaa agcatcacag gtcaccaaag tccaattaat gacttcgttg
    11161 ctgctactat cgctctttct ttaaatggtg gtgcgtacat tgctgaaatt gtacgtggtg
    11221 gtattgaagc tgttccttct ggtcaaatgg aagcaagtcg cagcttaggt atttcttacg
    11281 gcaaaactat gcaaaaggtt atcttacctc aagcagtacg ccttatgtta ccaaacttta
    11341 tcaaccaatt tgtcatctca ttaaaggata caacaattgt atcagcaatc ggacttgtgg
    11401 aactcttcca aactggtaaa atcataattg cacgtaacta tcaatcattc cgtatgtatg
    11461 ctatccttgc aattatgtat cttgtaatca ttacccttct cacccgtcta gcaaaacgtt
    11521 tagaaaagag gcttaaataa tggcagaatt aaaaattgat gtccaagatt tacacaagtc
    11581 gtatgggcaa aatgaagttt taaaaggtat tgatgctaaa ttttatgaag gtgatgttgt
    11641 ttgtattatt ggaccttctg gttcaggaaa atcaactttc cttcgcacat tgaacttact
    11701 tgaatcaatc acaagtggaa aagttgttgt ggacggcttt gagttatcaa atccaaaaac
    11761 agacatagac aaagcacgtg aaaatatcgg aatggtattt caacacttca atctttttcc
    11821 acatatgtca gttcttgaaa acatcacttt tgctcctata gaacttggta aagaatctaa
    11881 agaagcagct gaaaagcatg ggatggaatt acttgaaaaa gttggtttag ccgataaagc
    11941 taatgccaaa ccagatagtt tatctggagg acaaaaacaa cgtgtagcta tcgcaagaag
    12001 cttagcgatg aatccagata tcttactttt tgacgaacca acttctgcac ttgatccaga
    12061 aatggttgga gatgttctca atgttatgaa agatttagct gaacaaggta tgactatgtt
    12121 aattgtgaca cacgaaatgg gatttgctcg tcaagttgcc aatcgtgtta tctttacaga
    12181 tggtggtcgt ttcctcgaag atgggacacc tgagcaaatc tttgatacac ctcaacaccc
    12241 acgtctccaa gacttcctta ataaagtttt aaacgtttaa taaaaaaaga tgacttcgtg
    12301 tcatcttttt ttatatcctg atcacaaaca aattataata ggataagttt tcttctaaaa
    12361 ttagcaaaac atcagctaga atgtttgaat aatgttgcac aagggctttc agtatgataa
    12421 tatagcagta tgaaagacta tattaacaga atattgcact ttattaaaga acatatgact
    12481 tatcatgtca actttataga tgacttttta gatattaaat gggaaa
//
LOCUS       DATRNX010000053        12106 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRNX010000053 DATRNX010000000
VERSION     DATRNX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..146)
                     /locus_tag="VBC81_001209"
     CDS             complement(<1..146)
                     /locus_tag="VBC81_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6912255.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQL"
     gene            362..2863
                     /locus_tag="VBC81_001210"
     CDS             362..2863
                     /locus_tag="VBC81_001210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6912256.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2953..3561)
                     /locus_tag="VBC81_001211"
     CDS             complement(2953..3561)
                     /locus_tag="VBC81_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6912257.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3654..5042)
                     /locus_tag="VBC81_001212"
     CDS             complement(3654..5042)
                     /locus_tag="VBC81_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6912258.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(5039..5724)
                     /locus_tag="VBC81_001213"
                     /pseudo
     CDS             complement(5039..5724)
                     /locus_tag="VBC81_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5957..10672)
                     /locus_tag="VBC81_001214"
     CDS             complement(5957..10672)
                     /locus_tag="VBC81_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6912259.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            10995..11687
                     /locus_tag="VBC81_001215"
     CDS             10995..11687
                     /locus_tag="VBC81_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6912260.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            11677..12000
                     /locus_tag="VBC81_001216"
     CDS             11677..12000
                     /locus_tag="VBC81_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6912261.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
BASE COUNT         3471 a         2129 c         2332 g         4174 t
ORIGIN      
        1 agttgcttat ttcgtttgac ttcttttgcg atagtagtag ggtgtttcag tagattaagc
       61 ccgatggctt taaaggtttc tcctctatcc aaaccagatt ggatgtcatt acggtctgag
      121 agagttaggt gtttgtgttt tgtcatgatg gataaactca tttctaaccc aaacgtctca
      181 gacttaattc caccataaaa atccttttca cactaacttc cggttttgga gggcagacgg
      241 aacttacttt gagaaattac tgtggggaga gaacctgctg ttgagggtat tttcaccttt
      301 tttattgctg tttttcgtgt tataataaga ggactatact tttttgacat ggagaagtgc
      361 gatgacattc tataatcaca aagaaatcga acccaaatgg caagcttttt gggcagataa
      421 tcacaccttt aaaacaggaa cagacgcatc aaaacctaag ttttatgccc tagatatgtt
      481 cccataccca tctggagctg gtctccacgt tggtcaccca gaaggctata cagcaacgga
      541 tattctcagc cgtttcaaac gtgcccaagg tcacaatgtc ctccacccaa tgggttggga
      601 tgcttttgga cttcctgctg agcagtacgc tatggatact ggtaatgacc cagctgagtt
      661 tacagctgaa aacatcgcta acttcaaacg ccagattaat gctcttggtt tctcatacga
      721 ctgggatcgt gaagttaaca caactgaccc taactactac aagtggacac agtggatctt
      781 taccaagctt tatgaaaaag gtctagctta cgaagctgaa gttccagtta actgggttga
      841 agaattggga acagctatcg ccaacgaaga ggttcttcca gatggtacat cagaacgtgg
      901 tggctaccca gttgtcagaa aaccaatgcg ccaatggatg ctcaaaatca ctgcctatgc
      961 agagcgcttg ctagaagact tggaagaagt tgactggcca gaatcaatca aagacatgca
     1021 acgcaactgg attggtaaat caactggtgc taatgtaacc ttcaaggtta aggatacaga
     1081 taaggacttc actgtcttta caacacgtcc tgacaccctt tttggtgcaa cttacgcagt
     1141 tcttgctcct gagcatgcct tggttgatgc tattacaaca gctgaccaag cagaagccgt
     1201 tgccgaatac aaacgtcaag caagtctcaa atcagacctt gctcgtacag accttgccaa
     1261 agaaaaaaca ggtgtctgga caggtgccta tgccattaac ccagttaatg gtaaagaaat
     1321 tccagtttgg attgcggact atgtccttgc cagctacggt acaggtgcta tcatggctgt
     1381 tccagcccac gatgaacgtg actgggaatt cgcaaaacaa tttaagctag acatcattcc
     1441 agttcttgaa ggtggtaatg ttgaagaggc tgcctttaca gaagacggtc ttcacatcaa
     1501 ctcagacttc ctagatggtc tcgataaagc gtcagctatt gccaaaatgg tcgaatggct
     1561 tgaagcagaa ggtgttggta atgagaaagt cacttaccgt ctccgcgact ggctcttcag
     1621 ccgtcaacgt tactggggtg agccaatccc aatcatccac tgggaagatg gtacatcaac
     1681 agcggttcct gagagtgaat tgccattggt tcttccagta actaaggata tccgtccttc
     1741 aggtactggt gagagcccac ttgctaactt gacagactgg ttggaagtga ctcgtgaaga
     1801 tggtgttaaa ggtcgtcgtg agactaacac catgccacaa tgggcaggtt caagctggta
     1861 ctatctccgt tacatcgatc cacacaatac tgaaaaattg gctgacgagg aactcctcaa
     1921 acaatggttg ccagttgata tctatgtcgg tggtgcagag cacgccgtgc ttcaccttct
     1981 ctacgctcgc ttctggcaca aggttcttta cgatctcggt gtcgtaccta ccaaagagcc
     2041 attccaaaaa ctctttaacc aagggatgat tttgggaaca agctaccgcg atagccgtgg
     2101 tgctcttgtg gcaactgata aggttgaaaa acgtgatggt agcttcttcc atgtggaaac
     2161 tggggaagag cttgaacaag caccagctaa gatgtctaaa tcacttaaga acgttgtcaa
     2221 cccagatgat gttgttgaac agtacggtgc tgacaccctt cgtgtttacg agatgttcat
     2281 gggaccactt gacgcttcaa tcgcttggtc tgaagaaggt cttgaaggct cacgtaagtt
     2341 cttggatcgt gtttaccgtt tgatcacgac taaagagatt actgaagaaa atagcggagc
     2401 ccttgacaag gtttacaacg aaaccgttaa ggcagttact gagcaagttg accaaatgaa
     2461 attcaacaca gctatcgctc aactcatggt ctttgtcaat gcagccaaca aggaagacaa
     2521 actctttagc gactatgcca aaggctttgt tcaattgatt gcaccattcg caccacactt
     2581 gggtgaggaa ctctggcaag tcttgacagc ctcaggtgag tcaatctctt acgtaccatg
     2641 gccaagttat gacgaaagca aattggttga aaacgaaatt gaaatcgttg tccaaatcaa
     2701 aggtaaggtc aaagctaaac ttgtagttgc taaagacctt tcacgcgaag aattacaaga
     2761 agttgccctt gctaacgaaa aagtccaagc cgaaatcgca ggcaaagaca tcatcaaagt
     2821 catcgcagta ccaaacaaac tggttaatat tgtggtgaaa taagataaag attttactag
     2881 aaatagtgat atttacgcgt ataaaatccc aaaagtttat gaacttttgg gatttttcta
     2941 tttttagcta aatcagtcag ctcgtactgt ttttttagca tagtaagttt ttaatacctc
     3001 ttttgtaata ccatgttttt gatagatttt ttctattttt gacgttaata tttttagttc
     3061 tttattttgg ttttcaatag agttatcaaa tttttgatag cggtcgttca accttgtctc
     3121 aaggtaagca cgttgttcaa aatataattt taatctatct ttttcgttag tggatagggt
     3181 tgtcttatca atcaattttt tgacttcact tgtgctatcc catgtttgat tatcaataga
     3241 agtatagaat ttagttagta gacgtttatt ttgcttctct agttggttga tatctttgtt
     3301 aatatacaat tcaataactt ttaaatcttt tgcttttttg acttcaatat gtcgttggat
     3361 atggtcgatt ttagcgtaga gaggagcagc ttctttatct gcaactttta acttatgttc
     3421 aatagttttt tgactcatat gaacagttga tgttttttgt gattgttcag ccgctacatg
     3481 atttggttgt aaactcgtat atgttgctaa agagagaacg gcaataagcg ataaggaatg
     3541 tattttttta gaatgtaaca tataaaaact cactttcttt tagaataact ttttgatcta
     3601 taggtattat aggttaaaaa agcaataact tctttgaatt tttgtaggaa aagttaggca
     3661 attttgtggc aattgaaata aatcgtaaaa gttgttgagg tcgaatcact ctcagcgatg
     3721 atattaccat gatgttgctt gataatttct ttagtcattg caagtccaat acctgtttgg
     3781 ccgccatgtc ctttatagaa gcgatcgaaa atataaggca agtcttcttt tgaaatggct
     3841 ggtccgtcgt ttgagatagt tagtttttga tttgtgtaag agatttcaat atgagaaaca
     3901 gcatagcgta gtgcgttact aaggatatta ttgatagctc gttgaatcaa ctcttcgtta
     3961 ccttttagta acttttcatg cttgtctggt ctataagtga tactaatctt ttgtttatta
     4021 gctaggggtt tgaaataagc aataatacta ttaagtaatt catttaaaga aaattcatct
     4081 ttttgatcac ttaagttact atctaattta gagagtaaga taatatcgtc tacgagctgt
     4141 ttcatttttt tgctctcttg cagaataaca gagtgtgcga gctcggcatc aataataccc
     4201 tcttgaactc cctcagtata tccttggatg gacattaaag gtgtccgtaa ctcgtgggag
     4261 gcattttgaa agaagtttct ttggctctct tctgcctggt agattcgatt tgccatttct
     4321 tggctctcaa gtagaagagg atcgaaatca gaaaagacaa tttctttttg actagattga
     4381 tgattgcctt gatttttcca aaggctggaa atataagttt gtactgattg aatagattct
     4441 ctaattccag tgaaagtctt cctcataata aatagcatgg gaatataggt tagtactaag
     4501 ataactataa gaaaatgatt aattttagta attagatgct gggtgtaagt aatgtctgaa
     4561 aagttaatca catgaaaaag ttgcgactgt gctttaggag ctttttttat gctaccatca
     4621 acaaggatac cagagtagtc tttggtcatt acataatagg tttttttctt gatggttata
     4681 aacataccat ctttttcctt ggaaatttgc cacaatcttt tttgggtaag atagcgaata
     4741 atatcatggc tctctttttt atgattatca gtgccatatt tcatacgata atgctggtca
     4801 aaaatagcat agtaagacca aacaaagatg ctatcttggt taaccatatt ttcatctggt
     4861 attttcttgt tgttatagat actatctaaa atggtgaaat tcgcctgcat ggaattcctt
     4921 gtcatttgat ggacatagtc tttaatgaca tagttgacag aaaaatagat gaggtaaaaa
     4981 ccaagaaata gagtgatgct attaataagg ataagattgc taattaaact tttctttttc
     5041 atttgtcctc tttgattaat ttgaagccgt aaccccagac agttttgata ctaacctgac
     5101 tgtggtattg cttaagtttt ttacgtaatc gtttaatagt gtcatcaact gctctgcttt
     5161 caatatcatc aaatccccaa atacgattaa gcagtgtttc cctagaaaaa gcagtttcag
     5221 gtttttcaat taatactaaa agaaaatcaa attctgtctt ggttaagggc atttcttcat
     5281 ttgataatag ggctattcgc ttttcagtat ccagaattaa atctagaact gatactgagt
     5341 gtcatttttg attgcttttt catcaaggcg tttgaacaat gctttaacat gtagactaag
     5401 ttttataggt gaaaaaggct tggtgaagta atcgtcagtt cctaaattaa aagccgtaat
     5461 gaaatcaaca tcagaatctc tagctgttaa catgataata ggaatattat catattggcg
     5521 gatgcttttc ataacatcta aaccattagt tccaggcatc attatgtcta atataactaa
     5581 atcgggtttt tggtttttat aggcttctaa gagaaggtct ccagtttcaa atgctcgaac
     5641 agtgaaacca tcatgttcta aaaaaggaac caccaagtca cgaatattct tttcatcatc
     5701 agcgaaataa attatttttg tcatgtatac ctcaataccc atagttataa tttgactttt
     5761 agctagctgt ttatgttata tcaaatataa caaaaaaaga gtttttttcc tattctttga
     5821 agtgttatag tgttataaac caagatattg cagagaaaag gtctttagga acatcatttt
     5881 aagctcctca aaaaggcacc agtcactaac tattaatagc tagtaattga tgccttgctc
     5941 tattttttaa agtattttat tctcttttga tagactttag tttgattgtt gccagaccta
     6001 gcatagagag actaaatagc aatattgtat aggttatttt aggagtgctt tcgcctgttt
     6061 taggaagctt gtttgtcttt atcttttgat tatcggtttg gctagtcacc cctaagacac
     6121 gcttctgagc aacctcactt tctttagtca tgcctttttt gaatatcaac gcctggggct
     6181 gatttggcgg attgataggt gtggtattga gagagtcatt atcattgagt gattgactat
     6241 aattctctgc ggaagaaact ctatcgcgac catcaagttc ttgttcggtt gcttttattt
     6301 ggtttaataa ctcttcaact tgagcttgtg tagcttgtct attggaaagc agcaattctg
     6361 ctttcttgat aaggttatta taattttctt tagcaaattg actggcgttc tgatatttag
     6421 atgatgtttg tttcagaaca tttgctttaa ttatcatatg gtgtaaacca gagtaatcag
     6481 tttcatgacc atttaattga tttttagcaa tatcaagatt ttctaaggct ttgtctactt
     6541 ctgattgagt gacgttaggt ttttgtagaa gatttttcgc caattggact tgattgatat
     6601 aagttgattg gcttaatgga ctagcattaa caaattgata gtttcccttg atttctgttt
     6661 cagctaaaat agcttttgcc agtaaagaag tatctgtttc tttaccgtta agagcttggc
     6721 gactttctct caaagcagca atagcattat caatacttgt ttgttccact ctatttttaa
     6781 ttaaagtttg tgccttttca agattagcta ggtaattatt ttttaagtga gttccagcat
     6841 tataaaacac aggttgggaa ataatgtcag tttgttctgc tagggcatta attagaggag
     6901 ctttattaat cagcgtcaat tttgttagat ttacttgacc ttcttttacg gatacaagta
     6961 aatcatctaa gttctctaaa gtgctatacc cactaggcag cgttacgttt aaacgatagt
     7021 ctcctacagg tatgttttta ccatagtcag taggagaata agttgctttt ggtagatcaa
     7081 caacagtatt tgtcttagta accaactgga ctgttgctcc ttttggtaaa agcttgtcaa
     7141 attcaacgag taaggctgct ttcttgagta agttaacttt aaataagacg tctttaaggc
     7201 tatctttttc agaaatagtt acagtgacac tttggggact gattagattt gctcgttcct
     7261 catcgtataa gaataggtca aacgtatagg taccaaaagg caatttcagt aaattgaggt
     7321 ctttcccgtg atgttgcttt ttgatgacat tacctttgtc atctttgaca gagtaagaga
     7381 aatcaatatc gacattactg ttaagctcag ggctaaacac tttgacgttt actaaaccat
     7441 ttttattgcc aatattgatc aaatcttcga gttttgctga cactagattg cctgcataat
     7501 cttcaacagt gtagtagaca tgagagaaat cattaacctc tttaggtaga ataaagctac
     7561 catccttttc ttgtttgata aatactttgt tatcttcaac aaggataccg tcttctcctt
     7621 tttgacgtaa gacgctatta taatgaccat ctttatcttt ttttaagtaa aaaacttgtt
     7681 ctctaaagat accagattcc ccgtgttcta ctgcaggacg agctttgaag atacgtctat
     7741 ctttgtcata agttgctgtt gttagtgtag gagcttgtct atccaaagtg atatcaaata
     7801 ccatttgttg tggcttagag ccagggacat cagagtagta tgttaaaacg tatttgtatt
     7861 ttccatcttc taaaggaata ccatcagtag tggttccttt ccattctgtg tcatataaaa
     7921 atgtagattt tggattttca gtatttccgc tatagtaatt tttatctcct gcttggggag
     7981 cacttaccca tagtggtttt tgtaaattaa catcatctgc acgatagact ttggctctta
     8041 aattattgaa attacgtaag aaaacacctt tcagtgcaac ggcatcttga ttttggtcat
     8101 cattaggtga aatggctaga tgaggctggc ctttttgctc tagttgtaag atccattttc
     8161 catctataga cttatagctt cccaaggctt tcaattggat aggactagta ccgtctgttg
     8221 agtagatagg ctctgaactt gtagtcatta aggcagtata atcttctgaa ccaggaactt
     8281 cgtttgtttg tttcggttgg aaataaaatc ccttttcttt gttagcaaca agcttataaa
     8341 tggatttttc taaaacttct aagttttgga attctccctt aaatccaaca taaggaatac
     8401 taagaacttc cccaccatca acagggtctg taaaacgtac gtagccttct aggaaatagc
     8461 cgtttggcat tacttttttt aacatgtcat ggtatttcga aacatctata tcaattgtaa
     8521 tcgttttagt ttgccctggt tcaatccgta tcgttttccc tgtaaatgta ccaagttgtt
     8581 gaggagccaa tgtgacaaag ccatctttaa cttgatcagt atttaaatag gttgtatagt
     8641 gtaaatcttt tgcaacttta ttgatattat gaacagtaac atcaaaggaa attttatctt
     8701 taatatttcc taatgtaacg ctaccatagt tgttttcccc accagttagg tataatcctg
     8761 tctgaactgc tgctgcgaca tcgataatac ctgctccttg ttggcgtggt gaggtgtaag
     8821 cgcctgtatc tttgtttagg tgtgctttag cagtactcat aagaaggtat ttgacagttc
     8881 tttcaatgtc acctttttta agttctggat gttctttcaa taagtattgt ttaactagag
     8941 cagtagcacc agcgacatgg ggagaagcca tacttgtccc actcatcata tcatattcat
     9001 tatcattgat agcggcgtaa atagagcctc caggagcaga taagtcaggt tttaattgtc
     9061 cgtctgctgt taatccccag cttgaaaagt cagataggac acctgcatta gggttggctt
     9121 gtttattttt gatattgtta aatacaattt tatagttgtt tttagctaaa acatctccaa
     9181 actctttttg agtaaaaatg gcaggaatag cgcttgcttc aggatctaaa ctcattgtta
     9241 gattagcttc tccagctttg ttattaaaga taatagctcc cacagcaccg tgattaatag
     9301 cgttgacgac tttttttgta aaagtaatat ctccacgttc aattaaggcg attttaccat
     9361 ttaaagtttt gtccttataa tcattgtcgt ttccttttcc tacgaaaaca taatcatatt
     9421 gcttccctac ttcaaaggtc ttatcactaa ctttaggatc tgcataagca gctaatccgt
     9481 tgttgagatt tttgttgttt tctaatccga taatgttgaa gactttatta accagtgttg
     9541 tattattata tgatgctaca gaaatggcct cacgagctgt tgatggacta ccaactaaac
     9601 cataatcagg ataaagagca gaaggctttg atgctccact cccaaatgta ccgtcgttac
     9661 ctgctgctat aacaacagaa acgccagcga gtctagccat ctctaaagct tttataagtc
     9721 ggtcatcggc attaactaaa gagccattag ctccacctaa acttaaatta atgctatcag
     9781 caccgagttt aacggcatct tcaatagctt taacataaag ggctggtcca gttcctcttt
     9841 tttcatcaga gaagactctc ataaacatta cttgtgcttc aggagcaaca ccatagatta
     9901 attcatttgt atctttcttg ctaggattag ctgttgcgat actggttacg tgcataccat
     9961 gagaagtgct tttcacctct tttagctctg tattgacatc aacatagttg tgaccaaaga
    10021 ttactttgtt gttataccat tttccatagt ttataccagc ttttgcttta gcagcattca
    10081 tttgctgttc gttttggtat tttgcttttg tcgaatcatt taattggaga gcatcgtgat
    10141 tggtatctag tcctgagtca ataattgcta caacagttcc ctctcctttg tagcctgtgt
    10201 tccatacttt ggggacagtg ataattttat tactatcaat gttttgaggg gtttctttag
    10261 aaatagctgg tgtaggtttg actgctaaat tttgtttggt taaaggagtt ttagaaactt
    10321 cttgagtgct ttcaaccggt tgatttgcgg ctggggaaaa tgtctcggtg gcttgagagt
    10381 gaggctcagt tactggttgt gtttctgttg gactattatt attagccttt gtaacaagat
    10441 tgtcttgagc tgttgacgca agggctgatg acgtgttgtt agtaggtgtc tcatttgcta
    10501 tgctcgttgg actgtctttt acttgctgtt caactgcaat atcagatggc ttatttatga
    10561 cagaatcagc agctacattt gtgataccgc ctaaaaaaaa tagggttccc aaaagtacag
    10621 atacggcacc taacttatat ttccggattg aaaaacgctg ttttgtgttc ataaaaatct
    10681 ccttttcttg taacataata attacaaaaa ctattaaaac ataaaaaatc aaaattttct
    10741 acaattttct gaattttcat tacaaattat tttgtggtca atttaagacc gtacctaaag
    10801 cattttttaa aaatcattct atgaaaataa taatattgtc caactatatg gctacaatat
    10861 caaaggttta tagaataatt acaggatatt tattagtcta attgctggca aagctaaata
    10921 atcattcctc ttctttctta gtaatcaccg ttgtgctata ataactgaat atactgaaaa
    10981 tttaggggga tatgatggaa aaaggattta aagagggagt gaaagatgcc ttgcccacag
    11041 ctttagggta tatctcaata ggcttggcgt ttgggatagt tgcttcagca tcagatttat
    11101 ctgcaataga agttggtctt atgagtgcct tggtatatgg gggatctgct cagtttgcta
    11161 tgtgtgcgct attattagca aaagcagact tgatgactat tacgatgaca gtatttttag
    11221 ttaatttgag aaatatgtta atgagtttac acgcgactac tatcttcaag tctgctcacc
    11281 taatgaatca attagccata ggaactctaa taacagatga gagctacggc gttttacttg
    11341 gagaagcatt gcatcacaaa gtcgtatcgc ctagttggat gcatggtaat aatgtgatga
    11401 gttatttgac atgggtaatc tcaacgataa taggaacttt actggggagt actattccta
    11461 atccagaaat gtttggttta gactttgcat tagtcgccat gtttataggt ctctttgtat
    11521 ttcagttgtt tgggatgtta tctgatggaa agcgacttgt tgtatatgtt cttgcttctg
    11581 taggtttaag ttacttctta ttatccactt ttttgtcagg agccttatca gtattgcttg
    11641 cgactgttgt tggatgtagt gtaggagttg ttttagatga taaatagtac cgtttttctt
    11701 gctattcttt taggttttgt tgtttcttgg gtgccaagag tcgctccatt catatttgta
    11761 aaatacaaag ggctaccaaa tagtcttgtg aaatttttaa aatttcttcc tctttctatt
    11821 ttatttgccc taacattctc tagctttttt tcagttaaat taggacaatt gccaacacta
    11881 aaatggttag atttattggc tggcatacca actctagttg tcactattcg atatcgaaat
    11941 ttattttaca ctgttcttac tggtatcgtt actatggctt tattgagatt actattttaa
    12001 cgtgtaggaa tggaagatta ataaaaacac aaaaaacgca tctatttcta gatacgtttt
    12061 ttgtgttttt gtctagacag gaacttttga tagccgatgt gtaata
//
LOCUS       DATRNX010000054        12066 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRNX010000054 DATRNX010000000
VERSION     DATRNX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            45..683
                     /locus_tag="VBC81_001217"
     CDS             45..683
                     /locus_tag="VBC81_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6912262.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVASISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(761..1546)
                     /locus_tag="VBC81_001218"
     CDS             complement(761..1546)
                     /locus_tag="VBC81_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6912263.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            1807..3252
                     /locus_tag="VBC81_001219"
     CDS             1807..3252
                     /locus_tag="VBC81_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6912264.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(3309..3980)
                     /locus_tag="VBC81_001220"
     CDS             complement(3309..3980)
                     /locus_tag="VBC81_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912265.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(4077..4397)
                     /locus_tag="VBC81_001221"
     CDS             complement(4077..4397)
                     /locus_tag="VBC81_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6912266.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(4406..4534)
                     /locus_tag="VBC81_001222"
     CDS             complement(4406..4534)
                     /locus_tag="VBC81_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6912267.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLTIGKSKAIIIVSTQ"
     gene            complement(4635..5429)
                     /locus_tag="VBC81_001223"
     CDS             complement(4635..5429)
                     /locus_tag="VBC81_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912268.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(5439..6668)
                     /locus_tag="VBC81_001224"
     CDS             complement(5439..6668)
                     /locus_tag="VBC81_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912269.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(6792..7853)
                     /locus_tag="VBC81_001225"
     CDS             complement(6792..7853)
                     /locus_tag="VBC81_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912270.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(7888..8887)
                     /locus_tag="VBC81_001226"
                     /pseudo
     CDS             complement(7888..8887)
                     /locus_tag="VBC81_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(8922..9953)
                     /locus_tag="VBC81_001227"
     CDS             complement(8922..9953)
                     /locus_tag="VBC81_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912271.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            complement(10092..10910)
                     /locus_tag="VBC81_001228"
     CDS             complement(10092..10910)
                     /locus_tag="VBC81_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6912272.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            complement(10931..12016)
                     /locus_tag="VBC81_001229"
     CDS             complement(10931..12016)
                     /locus_tag="VBC81_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6912273.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIELKEKMLTPN"
BASE COUNT         3510 a         2271 c         1901 g         4384 t
ORIGIN      
        1 catccaagtc tctgcacttt agttgatcaa taaaggagac tcctatgttt ttaatcgatt
       61 ttattttaca tattgatact catatctatg ctatggctaa cactgttggt aattggacct
      121 atctattgct cttcctcgtt attttcgttg aaactggggc tgtcatcttt ccatttttac
      181 caggtgatag tttattattt gctgctggag cccttgctgc aaatccaaaa atgtccttca
      241 atattgtaac ttttcttatt attttcttta ttgcagcatt tattggtgat agttgcaact
      301 tcttgattgg tcgtacattt ggttaccgtt ttatcaaaca tcctttcttt cgtcgtttca
      361 ttaaagaaaa aaatatccga gatgctgagc tttattttga gaaaaaaggt actgctgcca
      421 tcattttagg gcgctatatc cctattatca gaacctttgt tccttttgta gcaagtatta
      481 gtcagctccc tccaaaagta tttatcaaac gcgccttcat tgcagcatta tcttggtcag
      541 ttattgcaac aggttctggt tttctctttg gtaatattcc ttttgtgaag cagcacttct
      601 cgcttattat cttgggaatt gtatttgtta ccttaattcc tgtccttatt tctggtgtca
      661 aatcatatcg gaacactaag taatgtatcc aaaaatttct atttaacact attaatcact
      721 aagaatgaca acaaaaaaag agcaattagt tgctctttta ttaatttagt gttatttctc
      781 ctgttgcata atccatagtg tatgatgctt gtgtgtttgg aatagcaaca ttaaattcta
      841 atgtgccatc ttgtgattta gcttctaggt gcattgcaaa tggaactaaa tcagtatcat
      901 tacggtacaa tggagttaca cggtaacgaa cacgtttgtt ttggtcaacc gccttacgaa
      961 ctaagctttc ataataattt tgtccacgat tgattttttg atttgattgg ttggaatgag
     1021 ctgtttgtgt gacaacattt tgaggatttg acacggaagc atcccaacct ttgaaatttc
     1081 cagctaaagc ataggcaatt aaatgcccct tgtcgactgc atgcccataa tggtcattag
     1141 tagctacttg atgccaacct aatggtttcc aatttgcatc gggaagagtg gtatctctgc
     1201 gattttgata ttgacgaatg gttctatcaa gcattgcatt agcagttgat agtctttgtt
     1261 gattgtcaat tgtattttga acatacgctt taacattgac atcagcattt aaatttgtct
     1321 gattattatt taattcataa ataccattaa catattgaaa tggtaggtta ccaatagcat
     1381 tttttaaatt attttgaaat tcctggctcg tatcaacaga tgaattgaca tcagcatgta
     1441 ctatcggcgt tacgttttgc ggctgcgttg gaagaccaaa ggtcattacc cccaacatac
     1501 ctaatgtagc aattacggtt ataaacagtt tatgtaatct tttcataagc tttacctctt
     1561 tctaattaca ttaaacaagc gtctaccata ttgatatccg ttttagctaa ttgctctcct
     1621 tttgtcacaa ccatatcata tgcctattat ttaagcatgt caatcattta tttaatctta
     1681 tattaattta atattacttt aaatttaatt tactcgttaa attagaaaat ctgcaaataa
     1741 tagctttgtt catcccctaa cttaaatcaa aatttatgct atgatagtgg taaggaggct
     1801 tgttctgtga aaaaaacaat tggtattata ggtatgggag ttagtggttt agctgttcta
     1861 ctagcacttt ctcgtaaaaa agagaaagag ttaaaacagt tagctatttt ttgttttgat
     1921 gatactgtcc actttggacg aggaattcct tttcaagaag acgttgactc agctttaatc
     1981 aattccccaa tcaacgatat ttcatttgat tatcgccata tgtctgactt catggattgg
     2041 ttagaagaaa attactatga cacaaatcta acctatgtat caaggacact ttacggacaa
     2101 tacctttcag aaagaactaa gttactttta gataaactcc ctgtcacggt tattaaaaca
     2161 agagtggata aactaaacta tttatcagat gagcaacaat ggcaagtcta tattcaagat
     2221 aagctttttc ctgtcacatt tgatgaatta cacttagctt gtggtgctct gcctgttata
     2281 gaaccttatc atttggaagg gaagaccgat tatatcaaag acccttatcc tatcaaatac
     2341 ttggctcagg aaaattggaa gggaaaaaca gttgctatca tcgggactgg tctagctgca
     2401 attgatgtta taaaatggtt gctactcaat acacaatctt ctgttaaagc attttcaaga
     2461 accaactact tccctagcgt ccgtattgtt aatgatttac cacttacttg gcactttttt
     2521 accaatgata accttaaagc atttctatca catccttcaa aaaccttcac tattgccgat
     2581 tttaatgatt tgatgtctgg cgaattggag catcttggct ttaaaaactg gaaaaataca
     2641 acagaaactt ttttatcccc aggaattgat ggtctcaagt tagcctttaa ataccctaag
     2701 caactctatc tattacaaca attagcttca cgaatcactg tctggttaac tgacctttgg
     2761 ccactcatgt cattatcaga taagaaagag tataaagcac tctatggaaa agcgattatt
     2821 aacttgcgaa atcctatgcc tgatgaatct gctaaagtaa ttttgaaagc tgcacaaaaa
     2881 gggacttttt ctctgatcaa taacgtcaag acaattacaa aaaatgacga aggattttca
     2941 atagaaaaag aaaaacaaaa tcatatatct gtcgatcgcg tcattaacgc tactggttac
     3001 cagttaaaca ctaaaaactg gacaaaagcc aatccacttc taaaatcgct catcaatcaa
     3061 gaattttgtc aaattgacga tcaaggaggg attactattt taactgaaaa tgcacaagtt
     3121 ttgtctccaa aatatggtcc aatgccaggt ttatatgcgc atggtgcact ggttaatggt
     3181 gtcgtttatc agaataactc tacaattaaa attcaacaaa tggcagagcg agccttttct
     3241 gataataaat aaccatttct aaaaaatacc tcctctttta ttatttaaga aagggaggta
     3301 tttttttatt ataaattcct ttgttgtaat atagactgtg ctatactttc ttttgataat
     3361 tcttttaagt tatctcctat caattgtaaa gcagtatcag ccatatgagc gatctctcta
     3421 ctatgtgtca ccataataac acatcgtttt tccttatgcg caaaatcttt aagacgcatc
     3481 gtaatatcac gcgcaatgtc aaaatctaaa tttcccgtag gttcatcaag taaaatagct
     3541 ggtgttccta caaccaaagc tctcgcaata gctacacgtt gttgttgccc tcctgataat
     3601 ctcaagatat ttcgcgttaa catatcttct tttaaaccta aatccaacaa taattgttta
     3661 tctgcagtag gttttaccaa ctgaacattt tccaatgggg ttagatagtc taaaaggtta
     3721 taattttgaa agaccaaagc tatattcttt ttgcgatgct gagcataacc tgcttcttta
     3781 atatcttttt tgttaaataa tacttgtccc tcagtaggcg aatcaagacc tgccatcaag
     3841 gatagaaggg ttgttttccc tgaaccagaa ttccctaata tagtataaaa tacccctgac
     3901 tgaaaactat aattgatatt ttctaatgtt ttattgtcgt tccccttata acgataagca
     3961 atatttttta attcgagcat agatttctct ttcttgagct atgtattcaa tagcagagca
     4021 tattcatttt aaccgttaat atagatgtca ctattggtcg gtatacttat ggcactttag
     4081 gacagttttg ataagattcc ccttggtttg tagcgagcaa taattaaact agtagtaata
     4141 atagcaaata agacgattgc taaaccaact agaattacta accagacttc ctcaggcttc
     4201 acttgtacat ttaacgtctt aatcgtttgc ataaacatat ctgtatcact atcagcccca
     4261 agctgcatat tcccaaaccc tttcataaag tctgctctga cagttctaga ggcctgatac
     4321 actaaggagt ctgctaattt ttgaccaata aattgactga taagtactga taaagcaaag
     4381 gctggaatag caatcattag tacttctact gcgtactgac aatgataatt gctttagact
     4441 taccaatagt taataaaata cctgtctcat gggttctacc accaatccag aatagcagag
     4501 ccatcatcac taggataata gatactacta acatgccgcc ttgtagagaa gtaattaatt
     4561 gattcaagac atctatactt tttgcataac ttgttagcaa ggttgctgta attgtcagtt
     4621 gatatttatt ccaatcaata ggattgtttt ttgataaaac ttttaagagt ttctatatca
     4681 gcattctttt taggatatac agttgtttgg cgataaaagg ccttaccttt agaatagccg
     4741 tataaatcct taatcaattg cgtatctgaa aaggctgtat tttctagcat ctcataaggg
     4801 taatttgatt tttgtaccgt tttaccttta tatatgccaa caatagttgc ttcaactaat
     4861 gttttatcct tactgtttct aacggggtct tttgaaaaca ttaacttatc gccaacttta
     4921 agatgatttg tcttcgcaaa agcctgatga acaattataa ctcgctgatc tttcggcgtg
     4981 atatgtcgcc ctcccttaag agaaatcatc cccgctgtgt atttaggatc tgtatcggag
     5041 taactattac catgaacttg taaaacttgt tctttatatt gttctttatc atttggtcgt
     5101 gtaaccattt tttccgaggt tagtccagca taagccatca aattattatt aacgcgctta
     5161 acatttggat tacttgctat gttaactatt gctttttcag ggatttctcc agatccgtaa
     5221 ccatcaccaa caaggtcagt actatccgat gaaatatgca aaattgatcc agtttgcctt
     5281 gaaaatgtct tcttagcagt aagaccagca ttttttaccg caataccgct aattaatgta
     5341 gaagcaacta gccataaaca tacgaataaa gtgatactct tacgcttttt acggctaaca
     5401 tataaaaagg aacgtttaaa taaattcata ttaacctcct atgacattcg tgacaatatt
     5461 tgctttggtt gtagtttaag aatagaataa ctagataata caactgcaaa taagcaaatt
     5521 aaagcaccct gagcatagag tctaaagaca tccattactc caacagaaac agcgaggtta
     5581 tcaattctat caataattag actactattt tgtacaattt gtgaactcac accaccatta
     5641 acttgtgata aaaggccagc tgcaaatgtt tttccaagaa agacaccacc aataatagat
     5701 agtgtgaagg cagcacctgc tactaacagt acttctatca gataatgacc tatgatttca
     5761 aacttagttt ttccgatagc gagtaaaatt cccgtctctt taatgcggtt attaatccaa
     5821 aatgttagca tgacaactaa tattgccatc gccacgagac taacaataag aatgattcgt
     5881 cccactagac gatgcaatgt ctcgatatta cgataagaat ctccataaaa ctctttagct
     5941 gtttgaacat ctaatttccc gaaatcaagc ggccattgtt tgatgttttg gataactgta
     6001 tccactaaac gtttatcact taagcgcact gcaaccgtct gataaccttt ttgtttacta
     6061 atcttcctaa ccaatgttgc ctgagcaatc aatgtattat caatattggg attaaaagta
     6121 tttttagact tagcactatt cgcatcataa attcctgcaa tagtaacatt ttctttgcct
     6181 aatctcaatt tgttacccac acttaaatga ttcttttgtg ctaacttcaa tggaactagt
     6241 acttgttttg tattatcggc cgctaaatgg cttcctttct ttaagaccaa tgcttttgtc
     6301 tgaaaagctc gtaaatcttc tgtactttga taacctgcta atgtaacttg attctcttct
     6361 ttagaagagt tatatggtga tacagatttt aaacggctac caacatcaat gcttaaactt
     6421 gcctctactg actcaaccat ttttgctttt ttcaaagctt cgtatgcttg ttcaggccaa
     6481 taagcctttt ctttggaggt taaataaatg cttttccctt gttttgttag gatatttttt
     6541 tccaaagaat gctgaatact catcagactg attaaacatg tgcctatagt tagaaaaact
     6601 aagaacaagg ttactgtttt cattttttga cgtttcaaat aagctaaact taatgtccat
     6661 aggttcacta ttttccctcc tttttctatc ttaataatct ctagaataac gatagaagtc
     6721 gttagatatt ctctatcgac ttctacgtta tctatcaatt agtgatttta tagcactgtt
     6781 tctaaaaaca attatttctc aaatgttttc ataaattggt tagtataatc aattacttct
     6841 ttaaggttag tatcataaat ttggtgagtt ccgtctagtg tatgaataga gacttctttt
     6901 ggatcttctg ataactcctt atgttgctct tgccaactgc ctttaattgg tatatctaaa
     6961 ctacgtaatt gaagccctaa gtcactttca cgtgctaata aatacatagc aggaattccc
     7021 tttggaatct tatagttggc actttcgtta atcatattag aagaattctt cgcttcttct
     7081 ttcatattag aattattaag tatcttatgt ccaatagcaa aaaattgtgc attaatatcg
     7141 tcggatactt taggaatttt aggtaattct tttaacactg gagcagcgaa atcaacttgc
     7201 ttaccacctt ccatacccgg cgttgatgta tctaaaccta ttaatcctag cacttccttt
     7261 ggataagtac tgatgtattt aagggcataa acaccaccaa ttgaatgaga ggcaattaca
     7321 tattttccgg taataccaac tttttgtaaa gcttcgtgga tatctgaggt aatattagct
     7381 agatttcgtg gttggtcaat gacatcactt aaacctgaac caaatggctc aactaccaca
     7441 accttatgag ttttacttaa attactaatc aaattatggt actgatgttt ggcagtcacc
     7501 gtcccttgac caggaataaa gacaattggt actttacctt ctccaacagt atagacattc
     7561 atcttcttgc ctttgacttc aaccttttga ccatattctt tgataggtgt tttgatatat
     7621 ttttgatatt gtttgttact aataaagctc tctgtgcctt tattatattt ttcaagttct
     7681 ttcgttaact tcattcgctt ggctgctgtt gccttgtctt ccttcggatt tgcagttacg
     7741 ttttcttgct taggatcctc agttttagtt ttttgattat ctttattggc agaacatcca
     7801 acgcaaagca ttaaagtcag ggcgatggtt gataatgtcg ttattttatt catcttttct
     7861 ccttagtaaa ataattgggg ggaataatta tttttcatgc gattgaatcc actcgtcaat
     7921 agccttcgcc atatgtttat attgcgtatg ataaatcaag tggctagcat ctagtgtctc
     7981 aacagaaacg tcttttggat cttcagataa gtcaatatgt tgttgttccc agcttttaag
     8041 cttagggaat atttttttac gcatttttat atcttctgct gactcattag ctaagaaata
     8101 cttagctggc atacctttag ggaacttaac tttttccatt ttatgaagat tgtcactaac
     8161 acgcctatct tcatcgcgct ctgttttatt acctgatttt ttagcaccga ttgataagta
     8221 ttgttcattg gttttcttat caacttctgg aatggattct gcccatttta aggttgaagc
     8281 tgaagattct ccagttgtgc catcaccttc catatttggt gtgcttgtat ccatgccaat
     8341 gaaacctttg acatcgttag aatatgtctt agcataattt agaccgtata tgccacctaa
     8401 cgagtgagcc ataagataga aatttttgac accgactttc tttggtaata ttttcaatcg
     8461 tacgtggctt atctgtcata tcactaagcc cataaccgag cggttcaacc gtataaattt
     8521 tatatttttt ccctaatcta tcaagcaagt ttttgtaagc ataacgtggt gaaatttcag
     8581 cttgaccagg gaagactact agcggaattt tcccttgacc cattgtatat atgttcattt
     8641 ttttaccgtc accgacatca attttttgtc cgtaatcttc aattggtttc gctttatatt
     8701 tagttaggtc aatagcacct ttagcaatca tagctttttc tgcttctctc tcttgttttt
     8761 gcttagcgat tgacttttct tgcttaggct gctcagtttt agttttttga ttatctttat
     8821 tggcagaaca tccaacgcaa agcatcaaag cgagggcaac ggttgataat ttcgttattt
     8881 tattcatttt ttctccttga cttaggtatt tcattcaacg attacttttg atgggattgg
     8941 ataaatccat caatagcttt agccatatgt tcatattgtg tttggaaaat attgtgacta
     9001 cctttaagaa tttcaacgga agcatctttt ggatcttcag ataaatcaat atgctgctga
     9061 tcccaatttt tcaaaccttt aaaatctgct tgacgtagct tgattccctc cacagattga
     9121 tcagcaagaa aatacttagt tggcatccct ttagggaatt ttacattctt caatttacca
     9181 agattatcac ctaagcgctc atcttcatct cgttgagact tattgccgtt aatttttttc
     9241 gcaattgaca gatactgttc gttagtctta tcatcaacat ctggtaattt cattgcccat
     9301 ttagcacttt cagggtcata ctttgttttt tgttcccctt ccatccaagg tgtactggta
     9361 tccataccaa taaatcctct gacttcttct ggataatttt tagcataatt taaagaatac
     9421 ataccgccaa gtgagtgagc tgcaaggtag aaatttttaa ctccaatttt atttaggcct
     9481 gtgtgaattt cttttgtaat gttttctagc gtacgtggtt tagtaggaat atcactgaga
     9541 ccatagccta atggttctac tgtataaatt ttatattttt tacttaaccg ctcaattaaa
     9601 ttcttataag catagcgtgg cgaaatctca gcttgaccag gaataaaaac aattggaatt
     9661 tttccctgac cagtttcata aatgttcatt ttcttgccat caccaacatc gattttttta
     9721 ccataatctt tgaccggttt tgccttatat tttgtcaaat caacagcgcc ttggggatgc
     9781 atctcttgat cagcagcttc tttgtttttg ggttcttctt tttgcttatc tgtgagttta
     9841 gtttgtttag gatgatgaga ctgtgtttta gatgtagacg tgtttgatgc agcacacgcc
     9901 gtacaaagta gtaggcttaa agcaacagtt gatagctttg ttatattctt catgcattct
     9961 ccttattgtc tggaaaacat agctttacca aagtgtcaaa cgtttttctg gttttagata
    10021 cattgaatcg ccttttttaa caccaaaggc atcataaaat tcttggaaat tacgtactgg
    10081 gatgttagct ctcaattcat atggtgcatg aacatctgac tgaattgagg acttactttg
    10141 ttctttggtt gctttttgac gccaaatact tgcccatgat tcaaaaaatt ctttatagtt
    10201 tggatttttt cagtcttaag agcttctaga gatgccataa caccaccatt atctgcaata
    10261 ttttctgcta aagttaattt accatcaact ttaccgccat ctgctttaag gccatcccat
    10321 tggtcaatca tagcttgtgt ttttttctta taatgtttta aatcttcttt agtccaccaa
    10381 tcgtgaagat tccctttctc gtcatatttc ataccattaa tatcaaatga gtgtgaaatt
    10441 tcatgaccaa taattgctcc gatagctcca taattttgac taactgtttt agttttatcg
    10501 tagagtggtg attgaaaaat cgctgctgga aagactatgg aattggtatt aggatcatta
    10561 taagcattta cagcattggc actcatttgc caatgttcac gttgattaga ctggttaaat
    10621 tcttcaaatg ttttcttgtt cgataatttt ctgtagttat cattgttttc aaagaagctt
    10681 gctttactat caaattggta ctgacgataa agatcaggat aatcttctgg atagccaatc
    10741 attaatctca tgttatcaag tttcttaatg gccattgctt ttgtattttc tgataaccaa
    10801 gtgttgtttt ttaaccgtgt tttatagaca ttaatgattt tcttagccat atgttcgacg
    10861 tcttttttag ctgcctcacc aaaatatttc ttaccataat aatcacccaa aatatggcca
    10921 aaagtatatt ctaattgggt gtaagcattt tttctttcaa ttcaatctga gtcaaaccag
    10981 acgcaacatt cttaaaagca ctcgctgctt gacgattttg ttctcctaga aaatctgatt
    11041 gatcaactag catagaaatc atcatccatg ctttcatatt ggctaattgt ttactattta
    11101 taacatcatt aacctgttta aaataagttt tatcttctac attgactgcc ttattcgttg
    11161 gcccaactag tttttcaatc aattctttaa agtcaaattc cttggcgtaa ttgtgaaccg
    11221 tttccatact ttcagggtta tatcgccccg cagctgtctc actttcagct gtgattttac
    11281 tttgatcaac ttgcgttttt tctgataaga gtctatcaaa tgctatagct tgtttaacta
    11341 gttttctatc ttcaatgtta ctttttccag cttgtttaag caaagccatt gctgaagtac
    11401 ggtaagctga taatttagcc tcaccttctt tattgccctt cttatattgg tcaggtgatt
    11461 caagtaatgc gggtgcttga cgaagcacca attgcttttg gctattatct cgagcattgg
    11521 tttccacagt caaaccaaat ggtaaaacaa aaccactcat cacaaaatca tgggccaaac
    11581 tttgaaagtc tttcattgaa gagactgctt caagtttttg taaaactggt tttagaggtt
    11641 tgagaccatt tttatctctt gttttaaagt ccataccttg tttatagtat gcaaccattt
    11701 ttttctgttc atcattggtt gtttcaatct ttcctgaggc cattttagcc atatcagaca
    11761 ctaacagttg ctttaattta tcctcaattt ctgaaaaact attaacacta gtctgccctt
    11821 gtttgagttt tgtattggct agccaattat cattgacagc cctgtagaga ttttctctcg
    11881 gactgacatt atttgctttc ttctcactgg cagatactgc atgtaacata gcaatatctt
    11941 taaccacata tcctgatata agacaagctg ttaaagtaag aacactcatg gattttaaat
    12001 aggattttct ttgcataaat gacacctctc tttcttattc acttttagta tagcaagtag
    12061 ggtgtt
//
LOCUS       DATRNX010000055        11818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRNX010000055 DATRNX010000000
VERSION     DATRNX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..970
                     /locus_tag="VBC81_001230"
     CDS             <1..970
                     /locus_tag="VBC81_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912274.1"
                     /translation="VLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLPVVNQIKPQ
                     TIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNPKQKVIFYV
                     HGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKKLYQNTLAS
                     VTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNNPKIEKVQK
                     KDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTHDIFYPDNQ
                     LLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(1029..1973)
                     /locus_tag="VBC81_001231"
     CDS             complement(1029..1973)
                     /locus_tag="VBC81_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912275.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(1984..2793)
                     /locus_tag="VBC81_001232"
     CDS             complement(1984..2793)
                     /locus_tag="VBC81_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6912276.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(2919..5156)
                     /locus_tag="VBC81_001233"
     CDS             complement(2919..5156)
                     /locus_tag="VBC81_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6912277.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(5140..5793)
                     /locus_tag="VBC81_001234"
     CDS             complement(5140..5793)
                     /locus_tag="VBC81_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6912278.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            complement(5893..6633)
                     /locus_tag="VBC81_001235"
     CDS             complement(5893..6633)
                     /locus_tag="VBC81_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6912279.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            6768..7532
                     /locus_tag="VBC81_001236"
     CDS             6768..7532
                     /locus_tag="VBC81_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6912280.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            7525..7791
                     /locus_tag="VBC81_001237"
     CDS             7525..7791
                     /locus_tag="VBC81_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6912281.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(7974..9560)
                     /locus_tag="VBC81_001238"
     CDS             complement(7974..9560)
                     /locus_tag="VBC81_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6912282.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(9795..10643)
                     /locus_tag="VBC81_001239"
     CDS             complement(9795..10643)
                     /locus_tag="VBC81_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912283.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQAMSSKQIKDKNSKEK
                     VITVATYSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEF
                     FMSIFNKENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLL
                     LQEKKLITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTK
                     INLVPKRDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPS
                     EADVAF"
     gene            complement(10659..11321)
                     /locus_tag="VBC81_001240"
     CDS             complement(10659..11321)
                     /locus_tag="VBC81_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912284.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            complement(11314..>11818)
                     /locus_tag="VBC81_001241"
     CDS             complement(11314..>11818)
                     /locus_tag="VBC81_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912285.1"
                     /translation="MRKDIAYIFQNHNLLAGESVYYHLALVYKLNHQKVNHDAINDIL
                     DFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILCDEISSALDTNSEKEIFNLL
                     SDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDTVVPVKATLNQLKSNYVDQV
                     KEFLLHD"
BASE COUNT         3958 a         2217 c         1800 g         3843 t
ORIGIN      
        1 tgtactacta cttctaatct tttttgccat ttttatggga gcatatgctt acacgcatat
       61 tgttgaaaaa agatccctaa ctagcaatac tattgaaaaa actctacctg tggtaaatca
      121 gattaagcct caaaccatta aagaatacca aaattactta actaaggtag ctaaacgtaa
      181 tgttcttcct gtagacattc ctcaggcatt aaataatgaa aaggtagaaa ttactgctac
      241 tgatggcatg caaacattca cttggaatga taaaaataat cctaagcaaa aggttatctt
      301 ctatgttcat ggaggatcat atatccatca agcttccgaa ttacaatata tttttgtcaa
      361 taaactagct aaaaaattag atgcaaaagt tgtctttcct atttacccta aagctcctac
      421 atataattat agtgatgcta tccccaaaat taaaaaatta taccaaaata cattagctag
      481 cgtcacatct cacaaacaga ttatcctagt aggtgaaagt gcaggcggag gccttgcttt
      541 aggtattgct gataaccttg tcacggagca tatcaaacaa ccaaaagaaa ttattttaat
      601 atcaccatgg ttagatattg ctactaataa tccaaagatt gaaaaagttc aaaaaaaaga
      661 tccccttctt aaagcttggc aattacaaca agtagctcct tactgggcaa atggaaaaaa
      721 gaattttaaa aatcctcaag ttagtcctct ttacagtagc caatttaaca agatggctcc
      781 aatatcattt tttataggaa cgcacgatat tttctacccc gataatcaat tattacatca
      841 aaaattagca aaagaaaaca ttaaacacca ctatatcgtt ggtcaaaaaa tgaaccacgt
      901 ttatccagtc ttaccgattc ctgaagcaga gactgctttc aaacaaatgc ttaaaattat
      961 caatcattaa ataaaaaggt ttccaatcaa gtttagttgg aaaccttttt tctattactt
     1021 ttgaatgact aattatcttg agataaaaac tctgagaata aaacttcttg actaatcttt
     1081 tcagctaaag cctgtttaaa agctaatttc cctaaataat aggatttgtg gtcaattgtt
     1141 gggagtttca aaagttgccc aactaataaa cattcctgac caataatata aggacatttt
     1201 ttcctacttt cttcataacc ttttattagc cctgccgcaa cttcatcact tgtagctaat
     1261 ataccatcta tactaacttc ttttaccaat tgataagata aatttagtcc atcattaaag
     1321 tcatgaacat tcccaactac catatatgga gaggatagtt gcccatagac ctcttgataa
     1381 gctaataatg cggattgata ggtagcagag gattcattat tccttgaaaa taataaaact
     1441 aagtgctcca aacctcgtaa cttcatatca gaaaatgcct ctagaaatga agaatagcga
     1501 tcaaggtaag cagaggatag atgattatat tcctgcaatt tttcacaaac tactattcgt
     1561 ccatattttg cataagtttc aataatgtcg aggctaattg ctcttgaagt gaatattaaa
     1621 gcatcaatag cttccatctt gagctgtttc aagtaggaaa gttctaattc ttggttgtaa
     1681 tccgaaggca tcacaacaag ttgatagtcg gtagttttag cagcatctaa taggccattt
     1741 attaattgtg taaaataagg atggcgcgtg tggggaatga cgacacctat tttttgtgtt
     1801 ttgcctcgac ttaaatcccg cgctagttga tttggtatat aatccaattc ggtgataaga
     1861 gccattactt tttgtcgcgt ctcatcagaa acatgtgggt gatgattgat aacacgggaa
     1921 accgttgatt tagcaacacc tgccttttta gctatatcac taatacttgt cattataagg
     1981 ctcctacttt aaaaattttt gaatcccata tcccactccg tcttcatcat tagacttagt
     2041 tagttgatat gctatcgctt tgatatcatc aaaagcatta tccataacaa tagggtaacc
     2101 taccatttca agcataggca aatcattatg tccatctcca aatgcagccg tttcttctct
     2161 agctaactgc tctttttgaa ggatgtaagc aattccttta gattttttgg ctaagagatg
     2221 tgtaatttca agatacgctt tacctgaacg ttgaatagtg atttctggta actccaaact
     2281 ttgaagatat ttttctaatt ctagcatatt tgcttcatcg aaagtaatca tcataatttt
     2341 aaaagtattt gtgtgacctt ctaaaaactg atcctcatta tgaataaacg ttgggcattg
     2401 ttgagttaaa ctatgttcgt atcggattcc ttcatctatt ttatcacaat accagttatt
     2461 aagatcgtaa taagataaac taacttgagg aaaatggaac cgtattcctc ttagcaattg
     2521 ttttactgtg ctttttttaa tgatttgtgt atgaataggt agaacttgat tattatctcc
     2581 tattctataa atcaatcctc cattaaaagc aacttggact cctcctaatt gaagtgcatc
     2641 tacggcatcc ttcatttcca taggtgccct agcagacact aaagttactg gaatcgctgc
     2701 ctcacgtatt aaggtagcgt tacttttact cacttgtcct tcactattta atagtgtacc
     2761 atccatgtca caaaaaatac gtttgatagt cataaaaaat acctctcttc ttctgaaatc
     2821 agtataaatg ctggaacccg ttccatgtca agcttaaaat tactgaatcc ttgcgaaaaa
     2881 tagattgtat ttaacagata cgcaatcatt ttaataaatc aacgaactgt ctgaatatgc
     2941 cacttccacc atcctgttag cctaattgta cctgattgat ccgttcggaa aatcttgctt
     3001 ttaatctttt ggaacctttg taaagtctct tgatgaggat gctggtaacg attattttta
     3061 ccagctgaca ctagaaccac actaggagac aactttttca gaaaggatag acttgatgag
     3121 cccttagaac catgatgtcc tgctttaagg atatctactg ataaattagg ataagcttct
     3181 aataactgct tttctccctc ttcctccaaa tcaccggtaa ataagaagcc ttttcctaat
     3241 aaatgtccat ataaaacaat tgaatcatta ttttttccat cacccttgtg ccatgggtaa
     3301 agtacttgta aataacttcc catgacagaa agtttatcac cagcttctat agattttaca
     3361 gctactccta tctttgataa acgcttaacg tactgcgaat tcgttaaaga acctgaactt
     3421 gtaatcaaac gtgcaacttt aaaatgcttt gagataactt ccatatcccc catatgatct
     3481 gtatcggtat gagttatcac taaattatct atcttgctaa tacctctgct tttcaagtaa
     3541 ggaatgagcg ttctctcagc attagactgg tttacttttt gtttccattc ttcaggctgt
     3601 tcaaatctca cccttccccc agtatcaatt aaaattgttt ttcctaacca atccctcact
     3661 aaaatactat ctccttgtcc aacatctaaa atcgcaacct cgttagtaat tggattctta
     3721 gtgataaaaa acaaggtaaa gataataaga agggagcaat aacgaaaaca tttagaatga
     3781 taataatcat aaagtaatcc caaaattata atcatgacta ttaaaagaaa aaggctgggc
     3841 tttccaaaaa taagtggtct tggaaacagt tgcccagtcc attttaataa tacttctagg
     3901 atttcaaaca aactattaat acaacttaat ttaacgaacg gtgataagac aaatataaca
     3961 gttaataaag gcaaaaatat aatatcaaat gcaaaagata aaagtgctgt taatataatt
     4021 gatattggtt gaaaacccga aaaatagtag gttagtatgg gaagaattcc tacagagact
     4081 gtcaaagatt cgatagctac tttttttata ctcgaaaaat gatcaaagga ggtcatcgta
     4141 agtataaaag cataagcaaa gctaagaact cctcccgtag tcaaaagtga gtgggggaga
     4201 gagataagac aaattaacaa gcagagagca aagttctcat ctttcttgac accgctatgt
     4261 gataataaag attgaataag tgccctaacg actgagatac taaagcctgt taaaccagca
     4321 taaattagcg agaatggtat ttgaagtaac caaacatggt ctagacgtaa gccaatacgt
     4381 agacagatat aacgaaaaat accgagaaaa aaacctactt gcatacctga caaagcaaat
     4441 aaatgtataa tacctagact actatataaa tcggacatgt catcaaaggt cttatctaga
     4501 tatccaaaca aaagccctga catatagtga cgcataggat ttggaaaagt ttgactaata
     4561 acaattgcac ttctacgcca cttaaacata agatcaaaga aagacctatg ttgaaataag
     4621 cgtatctgtt ttatctttgt cactttggcg atacgataaa ttccatgtct ttttaaatac
     4681 tgacgataat caaagccatt aaaatgcctt ctctcctctg cttcttcaag tttgatatct
     4741 gcaattatca ttatattatt atcaagattt tgaaaataat ctttctcact tttatttttt
     4801 aatgtgtaaa aagcttgata agtgttacct gaagcatctg cctggaatga taaaacatca
     4861 ccattgatag aaatagtatc aatacgcact ttaatctggc taatatgatt cggtactctt
     4921 tgatattgga cattcatatt gtgattttgc cataaaaaat aaaataagaa aaaaattact
     4981 attgttattg ttttaaaaat agtttcacga tcatattgat aaatcagaaa aataaaagta
     5041 aataaaagaa aggcacaacc aagcattgtc caagaaaaca ctagtaagta aatttggaag
     5101 accaacaatg ctaaataaat aggttttaga ggaaaatact tagtcaagtg taacatcttg
     5161 acgtaatttt tctagtgttt tctcaccaat acctgataca tttttgaggt catcaatagt
     5221 tttaaaacca ccttgactat cacgataatc taaaatatct tgagctcttt tagctccaac
     5281 gcctgatatg gtctgaagac ttgataaatc tgctttatta agattaattt tccccttact
     5341 atttccctga gtagcaatat tatttgcttt ctcctcttcc acaacagtta ttttttcacc
     5401 tttttgagca acatagataa ccgtctcatc ttgtaatttc tgtgctagat taacggattt
     5461 actatcagcg agattgctta gtccacctgc acgcttaatc gcatctgtta cacgactttg
     5521 agaaggcaaa ctataaacac ctggatgatt gactgcccct tttacgtcaa ccgttacttg
     5581 attagatact ttatctttga cgtgactaat cattggtacc tttttttctg ttatagttga
     5641 tacttttttc tctaaattga tatttaaatc gtcttcttta tgatgagtgc caaaatgatt
     5701 taaacccaaa attccaaaca ataagagtcc tacaataatt cctgttgtct cccatttatg
     5761 cgatttaatt ttttccaata ctatttcaaa cataaaaata ttcctccatt tacttattcg
     5821 taactaaaga gattttccta ttaaaaacaa aaaaaactat ttactaaaaa gtaaatagtt
     5881 tccatgtaac ccttatttta gatgtttttg tggatcccac acaaagcttg ctaaataact
     5941 aaaaattaga gttaggacaa gcaagacaat agctataatt gctaaaggta gacgataaat
     6001 ataagtcaat ggatttaatt tcttaccatc atcataagtt tcattttctc tatctaaacg
     6061 atcaaattct tcctgaatac gacgagatac ttctgcaatc ccttcggcat ccatacgctt
     6121 aatatcagaa acatcaatag ggttaccaaa attcatatca acacgatgcc cctttaatag
     6181 atttttaaaa accataggac ctctatatgc tgctggcata attctgactt ttgccatttt
     6241 tgcaataaca gctacgcctc ctttgacatc tttggagtgg cggcttccac tcggaaacat
     6301 caccaaagaa cggttactat ttttcagcat ctttacagga tatcgaatag cgtcttgccc
     6361 tggcttttca cgatcaatag ggaaagcacc gcacatttta atccaccaac caaacaaacg
     6421 attcgtgaat aattcttttt tggccataaa aacaaactgt ttgggtctag cagcaaaagc
     6481 catgtacaca ggatcccaaa atgttctgtg tggagctact aaaatataat tttcatcatc
     6541 ttttaacatt ttatcttcat tatgatagtg cgcattacca ttagccaccc aaatcaagaa
     6601 cataactaaa gtacgtaagt aagtatagaa catgtttctc ctctaccttt catcaagtaa
     6661 cttctatttt atcatgcttc ctttttttta gaaagaatta tcttaaaatc aacctccaca
     6721 ctttcaaaac tagtgctagg aagctgtaat tttgctataa taagcctatg atagatacaa
     6781 ttttaaaaga aaatgaacgc attgatcagc ttttttcaac tgatgtcaaa atcatccaaa
     6841 acaaagaagt tttttcctac tccatagata gtgttttatt gtctcggttc cctaaacttc
     6901 cttctcgtgg tcttatcgtt gacttatgca gtggtaatgg tgctgtcggt ttgtttgcca
     6961 gcaccaaaac aagtgcgaca attatagaga tcgaactaca agagcgcctt gcggatatgg
     7021 ctaagcgttc aattaagctc aataaattgg aaaaacaggt tactatgatt aatgatgatt
     7081 taaaaaatct tcttgatcat gttcaacgtt caaacgttga tttaatgctt tgcaatccac
     7141 cttatttcaa agcaagcgaa acctctaaaa aaaatctaag cccgcattat ctccttgcaa
     7201 gacatgaaat aacaacaaac cttagagaga tttgtcaaat tgctcaacat gctctaaaaa
     7261 caaaaggacg tatcgctatg gtacatcgtc cagaccgttt ccttgaaatt attgatacga
     7321 tgcgtcagtt caatttagca cctaaacgta tccaatttgt ttatccaaaa ttaggaaagg
     7381 atgctaatat gcttttaatt gaagctatta aagatggctc gacagaaggt atgaaaatac
     7441 ttcctcctct tgttgttcat caagataatg gagactatac tgagactatt tttgatatat
     7501 atttcgggga aaacggaaaa tctcatgatt aaagttcctg cttatatgta tgttttagaa
     7561 tgttcagatg gaacacttta tacaggttat actacagatg tcaagcgccg tctcaataca
     7621 cacaataccg gaaaaggtgc taaatatact cgtgcgagat tgccagtaaa acttctttat
     7681 tctgaagctt ttaatagcaa acaagaagca atgagggcag aagcattatt taaacaaaag
     7741 actcggaaag caaaattgac ttatattaaa caacataaaa atgaacaata atacgataat
     7801 atactaagtg caatgagcct gtcctcgctt ctttaacttt taaatttcaa cctcacttta
     7861 atcttttgtc ttcattctta aagttataag gcaattttac ggatatcact atctacgctt
     7921 tatcttaaaa aataaaaaag gagtcgctca gcaactcctt tttcgtaatc ctcttattta
     7981 tcgcctttgt tgcgaataac aaatccagtt tgtttttcac ttgaagtgtt tttatgaggt
     8041 tttttatttt cttgacgacg attatctttc ttgaacttat catcacgacg tttaaaatca
     8101 cgacgacctc cacgatcatc acggtcacga tttccacgac gatcaccacg atcattgcgt
     8161 cgacgatcgc caccacggcc attacctcgt ccacctttac ctttaaagcc accacctgat
     8221 ggtttaaatg gtaatggttt ttcacgtgtg atttcaactt ctggtaacga ttctggatcc
     8281 tgaactgtta agcttaagac ataaagagca agttcttctg gtgtatattg agttgctaat
     8341 tctaaagcat cggctttaaa tttatcaaat ttagaaacta attcttgatc ctcaaagtca
     8401 cgtgcaatac gtttcaatgc aacttttttc ttagcctgaa atgcttcgct tgcagtagca
     8461 ggcttcattc cagtcatacg ttttttagtt aagttttcaa taattgtcaa atatcccatt
     8521 tcgtttggtg aaacaaaagt gatagattga ccagatttac cagcacgacc agtacgtcca
     8581 atacggtgaa cataactttc tggatcttgg ggaatatcat agttgtaaac atgtgtaacg
     8641 cctgaaatat ctaaaccacg cgcagcaaca tctgtggcaa caagaatatc aatatggtca
     8701 tttttaaagt ctctgataac acgaagacgt ttattttgat ctaagtctcc atgaattcct
     8761 tctgctcgga aaccacgtaa ttttagacca cgagttaatt catctacacg acgtttcgta
     8821 cgtccaaaga caattgacaa ttctggttga tcaacgtcca tcaaacgtgt catagtatcg
     8881 aatttttcat tttccttaac gcgaacataa tactgatcaa cattcacatt agttaattca
     8941 gttgctttaa ttttaacatg ctcaggatct ttcatgaatt taaccccgat acgtttgatt
     9001 ggatcaggca ttgtcgctga gaataaaaga gtttgacgtg cttctggtac acgactaatg
     9061 attgcttcaa tgtcctctaa aaatcccatg ttaagcattt cgtctgcttc atcaagaata
     9121 agagtttcga tgtgattaag tttaagtgct ttacgtttaa ttaaatcaag taatcgacct
     9181 ggagttccaa caacaacgtg tgctcctgaa cgtaatgcct tgatttgttt ttcaatactt
     9241 gacccaccat aaacagagcg gaccttaaca cctttatcgc gtccaaaacg gaacaattct
     9301 tcttgtgatt gtactgcaag ttcacgtgta ggagcaataa taagtgcttg aatagtatta
     9361 tcttcagtat gaattttatt taaagttggt aacccaaaag ctgcagtttt tcctgtacct
     9421 gtctgcgctt gaccaatgac atctttgcct tcaagagcaa gtgggatagt catttcttgg
     9481 attggtgatg gctctacaaa gcctgctttt tctaccgctg aaagaatatc ttgcgataag
     9541 ttaagttctg taaatttcaa atgtttctct tttctaaaag acagtgcgaa gctgccttat
     9601 gaacctttat tagttatctt ttctgacaac ctacttagtg taacacaatt aaaagtaaaa
     9661 agatataaaa taaggtcaat ctaataaata acacgctttc ataaattgct agttctatta
     9721 gttgcttcag aaaatagaaa aaaggcaata aaaaccctcc atctataaag aaggagggtt
     9781 catagtttat ctatctagaa cgcaacgtct gcttcacttg gaatctcctt agtgatatag
     9841 tgacgaatct ctttacttgt tagtacttct ttaagtactt ttattttatc actattttta
     9901 ttatcttcac gagctaccaa ggcacctgca aaacggatat ctggtttttt ctcatataat
     9961 aatctatccc ttttaggaac tagattgatt tttgtcacat atccagggaa attaaagaca
    10021 aggtcatatt caaaataggc ctgattgagg ttaagtaatg ctacaggttc aattcgtaat
    10081 tttttagggt tagtaataat atcttcgata gccttggtct ttttggacgt attctttaag
    10141 gtgataagtt tcttttcttg caataatagc agagctctag tcatattggc aggatcagac
    10201 ggaatcgcta ccttagcacc gtctttaagc tcggcaatat ttttcaaatg ttgaccataa
    10261 aaaccagcca atgaatgata aattggtgta attgacacta gatgaccatc attttcctta
    10321 ttaaagatac tcatgaaaaa ttcatgttgt aaaaggttag catcatgttc tttgttttct
    10381 aaagcaatgt tagcctgaat atagtcagag accatgacaa cctttaaagt atatcctttt
    10441 tcttttacat tatctttaat caaatctaaa aaggtagatg taggtttgct gtaagttgca
    10501 acagtaatca ccttttcttt actattctta tctttaattt gcttagacga cattgcttgc
    10561 ttagacgaca ttgcttgctt agtacatgcc actaaacata aagacatcat gattagacat
    10621 gttagggaaa gtaatttttt catagttcct cttttctttt attttcttga gtagcggttt
    10681 gctataaaat agcccaaact ctgtaagata aacacatata ttataaataa gacaacaatc
    10741 aagtacataa ctgggtagtc atactcttga tagccaaaac gataggcata ttcacccaaa
    10801 cctcctgcac caataactcc cattattgtc gagtagccaa gaatacttat agccgttgca
    10861 gtaaatgata aaacaaggtc aatcttgatg gaggggatta aaaaataata aattatctgt
    10921 ctcttgttag cccctagact gagagctcga tctacaacaa cttgaggtat atttaataaa
    10981 gcttgttcca cataacgtgc atacaaactg actgaaacca aagtaagagg taagattgcc
    11041 gctatcgttc caaatgaggt cccaaaaatt aatcgattga gagggattaa gataaaaata
    11101 aaaataagga aagggacact ccgtaacgta cctaaaaata aatttagtag ttgataggct
    11161 aaagaatgtt taatcaagta agactttctc aatgaaaaca ataaaatacc tgttggaaaa
    11221 gcaattagaa aacaaagaat caaggttaac cctaacatta aattggtttc ccaaaatgat
    11281 tttaagatac cttcgccatg tttttcaata atgtcaatca tgtaacaaaa actcctttac
    11341 ttgatctacg tagtttgact ttaattgatt cagagttgct ttcactggaa caacagtatc
    11401 tacaatagtc tggtgatcca aaatcattac acgatcacaa tattgtttta aaagagatag
    11461 gtgatgagca atcatcaaga tagagatacc atatttttca cgaagatcag acaacaagtt
    11521 aaatatctct ttttcactat tagtatctaa ggcagagctg atctcatcac atagaataag
    11581 tttcggtttt tgcaaaacag ccatagcgat tgcaactttt tgttgctgcc caccactcaa
    11641 actatggcat ttcacctgtt ttaaatccat caaacctaaa aaatctaaga tatcattaat
    11701 tgcgtcatga tttacttttt gatgatttaa tttataaact aaagctagat gataatatac
    11761 actttctcct gctaacaaat tatgattttg aaaaatataa gcaatatctt tacgcatc
//
LOCUS       DATRNX010000056        11796 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRNX010000056 DATRNX010000000
VERSION     DATRNX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            143..871
                     /locus_tag="VBC81_001242"
     CDS             143..871
                     /locus_tag="VBC81_001242"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6912286.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            887..1444
                     /gene="frr"
                     /locus_tag="VBC81_001243"
     CDS             887..1444
                     /gene="frr"
                     /locus_tag="VBC81_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6912287.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            1562..2416
                     /gene="cvfB"
                     /locus_tag="VBC81_001244"
     CDS             1562..2416
                     /gene="cvfB"
                     /locus_tag="VBC81_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6912288.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            2554..3063
                     /gene="msrA"
                     /locus_tag="VBC81_001245"
     CDS             2554..3063
                     /gene="msrA"
                     /locus_tag="VBC81_001245"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6912289.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            3060..3275
                     /locus_tag="VBC81_001246"
     CDS             3060..3275
                     /locus_tag="VBC81_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6912290.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            3432..5204
                     /locus_tag="VBC81_001247"
     CDS             3432..5204
                     /locus_tag="VBC81_001247"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6912291.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            5328..6314
                     /locus_tag="VBC81_001248"
     CDS             5328..6314
                     /locus_tag="VBC81_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6912292.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(6405..7208)
                     /locus_tag="VBC81_001249"
     CDS             complement(6405..7208)
                     /locus_tag="VBC81_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6912293.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            7400..7876
                     /locus_tag="VBC81_001250"
     CDS             7400..7876
                     /locus_tag="VBC81_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6912294.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            8198..9004
                     /locus_tag="VBC81_001251"
     CDS             8198..9004
                     /locus_tag="VBC81_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6912295.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DLDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            9399..9896
                     /gene="ybeY"
                     /locus_tag="VBC81_001252"
     CDS             9399..9896
                     /gene="ybeY"
                     /locus_tag="VBC81_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6912296.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            9877..10275
                     /locus_tag="VBC81_001253"
     CDS             9877..10275
                     /locus_tag="VBC81_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6912297.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            10317..11216
                     /gene="era"
                     /locus_tag="VBC81_001254"
     CDS             10317..11216
                     /gene="era"
                     /locus_tag="VBC81_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6912298.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            11453..>11796
                     /locus_tag="VBC81_001255"
     CDS             11453..>11796
                     /locus_tag="VBC81_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912299.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFIIMKGCLTSISDILIYFYL
                     HLVVVYYTIGMI"
BASE COUNT         3947 a         1704 c         2350 g         3795 t
ORIGIN      
        1 taactgtttg accaatctct gaattattat gtttttctta ttttaggagt tgataaagaa
       61 atcaacttac tagtgtcatt gctctattaa aattttgcct tttatggtaa aataataaag
      121 acaaaataag gagaacatag tagtggaacc taaatatcaa cgtatattaa ttaagttatc
      181 tggtgaagca ttggcgggag ataagggtgt cggtattgac attcctaccg ttcaatctat
      241 tgcaaaagaa attgccgaag tacacaattc aggtgttcaa atagcacttg tcattggtgg
      301 aggtaatctt tggcgtggag aacctgcagc agaagcaggg atggatcgtg ttcaagctga
      361 ttatacaggg atgttaggga cagtaatgaa tgctcttgta atggctgata gccttcaaca
      421 atatggggta gatactagag ttcaaacagc tattccaatg caaactgttg ctgagcctta
      481 cgtacgtggt cgtgctttac gtcatctaga gaagaatcgt attgttgtct ttggagcggg
      541 tattggttcg ccttatttct caactgatac aacagcagcg cttcgtgctg ctgagattga
      601 ggcagaagca attttaatgg ctaaaaatgg agtagatggt gtttataatg ctgatccaaa
      661 gaaagatgcc aatgctgtca aatttgatga attaactcac gttgaagtaa taaaacgagg
      721 gttaaaaatc atggatgcaa ccgcatcaac tatttcaatg gataatgata ttgaccttgt
      781 tgttttcaat atgaatgaga ctggtaatat taagcgtgtt gttcttggag aacaaatcgg
      841 aacaactgtt tcaaacaaag catctgaata ggagtgtata aaaaatatga ctaaggaaat
      901 cgttacaaaa gctcaagagc gttttgagca atcacaccaa agtttatcaa gagaatttgc
      961 agggattcgt gcaggacgtg ctaatgctag cttgcttgat cgtattcaag ttgagtatta
     1021 tggagcgcca accccactta accaattagc ttctattact gtacctgaag ctcgtgttct
     1081 tttaatttca ccatttgata aatcatcaat caaagatatt gagcgtgcta ttaacgaatc
     1141 agatttaggt attaatcctg caaatgatgg ttcagttatt cgtcttgtta ttccagcttt
     1201 gacagaagaa acacgtcgtg atttagctaa agaagtgaaa aaagtcggag aaaatgctaa
     1261 gattgctatc cgtaatattc gccgtgatgc tatggatgaa gctaagaagc aagaaaagaa
     1321 taaagaaatt acagaagatg acttgaaatc tcttgaaaaa gatattcaaa aggctactga
     1381 tgatgctgta aaacatattg atgagatgac tgctaataaa gaaaaagaat tgttggaagt
     1441 ttaatagtaa cgtttgctat ctttattatg attggcaagt aataaaatga ttatggaggt
     1501 aactgataga atcaaaacca gatggatttg gtttgtcggt taccttttta aaaggagaaa
     1561 aatgaataca ttattagcga cagtcataac aggcttagta actgatgaaa acaaagattt
     1621 ttactttatc caaaaagatg gctttacctt tgcattatca aagtcagaag gagagcatca
     1681 cattggtgaa atggttaaag gattcgccta tacagatatg caacaaaaag cacgtcttac
     1741 aactaaagaa acatttgcga ctcgagatca ttatggttgg gggacagtta ctgaggtacg
     1801 taaagacttg ggagtatttc ttgatactgg tcttccagat aagcaagtgg ttgtttcttt
     1861 ggatgttctt cctgaattga aagaactttg gcctaaaaaa ggtgatcgcc tctacgtttg
     1921 cttagatgtt gataaaaaag atcgtctctg ggctctccct gcagatcctg aagtgtttca
     1981 acgaatggca acccctgcat ataacaatat gcaaaatcaa aattggccag caatcgttta
     2041 tcgcttaaaa ttgtcaggaa cttttgttta tctaccagaa aataatatgc tcggctttat
     2101 tcaccctagt gaacgttaca gtgaacctcg tttagggcaa gtgctggatg cacgagttat
     2161 tggcttcaga gaagttgata gaaccttaaa tttatcatta aagcctcgtt cttttgaaat
     2221 gttagaaaat gacgcacaaa tgattttaac gtatttagaa agtaacggag gattcatgac
     2281 tttaaatgat aaatcttctc cagaggaaat taaagctact tttggaattt ccaaaggtca
     2341 gtttaaaaaa gcacttggag gactcatgaa agctaaaaag atcaaacaag atcagctagg
     2401 tacagaatta ttataaactt ttgaactttt aaagttttct cacgaggtcg ttttagttat
     2461 agctagaatt aatggctaat tatagaaaaa agctgagagg aaattttggc tttttttgtt
     2521 ataatgtatc tattaaaaag aatggagaac gatatggaac gtgcgatttt tgctggaggt
     2581 tgtttttggt gtatggtaca accatttgaa gaattagatg gtattgaatc tgttttatct
     2641 ggatacactg gaggacatgt tgagaatcca acttataaag aagtttgtag taaaactact
     2701 ggacatacag aagctgttga aattattttt aatcctgaaa aaatctctta tgcagattta
     2761 gttgagttat attgggcaca aacagatcca acagacgctt ttggtcagtt tgaagatcgt
     2821 ggggataatt atcgtcctgt catcttttat gagaatgaag agcaacgtca gattgctcaa
     2881 aaatcaaaag ataagctaca agcttcgggt agatttgata gaccaattgt aaccagcatt
     2941 gagcctgctg atacttttta cccagcagaa gactatcatc aagcattcta tcggactaac
     3001 cctgctcgtt acgccttgag tagtgccaga agacatgcat ttctagagga gaactggcat
     3061 tgagaaaatc attttacagt tggttgatga cacaaagaaa tccaaaatca aatgaaccag
     3121 tagcaatcct agcagattac gcttttgatg aaaccacttt tccaaaacat agttcggatt
     3181 ttgaaacagt tagtcgctat ttagaagatg aggccagttt ttcctttaat ttaacagatt
     3241 ttgatgatat ttgggaagat tatctcaatc attaacaata atatagagat tgtattttca
     3301 atctcttttt taggttatag aaattggaca cattgtctaa gactgtctga aaagcaggtt
     3361 ttttaatggc aatcgttttc acaaaggagt agaatatagt catctaaaat atagccctag
     3421 ggaggttgtt tatgagatat acaaatggaa attttgaagc ctttgcaaga cctcgaaaac
     3481 ctgaaggtgt ggataaaaaa tccgcttata ttgttggttc tggtttagca ggattagctg
     3541 ccgctgtctt tttaatacgt gacggtcaaa tggatggtca acgtattcat atttttgaag
     3601 aactacctct ttctggagga tcacttgacg gtgtcaaacg acctgatatc ggttttgtaa
     3661 cgcgtggtgg tcgtgaaatg gaaaatcact tcgaatgtat gtgggatatg taccgttcca
     3721 tcccctctct cgaagttcca gatgcttctt atctagatga attttattgg cttgacaagg
     3781 atgatcccaa ttcatctaac tgtcgcctca ttcataaaca ggggaatcgc ttagaatctg
     3841 atggtgattt tacactcgga acacattcca aagagttagt taagctagtc atggagactg
     3901 aagagtcttt aggtgctaag acgattgaag aagttttttc aaaagaattt tttgaaagta
     3961 atttttggac ttattgggct actatgtttg cctttgagaa atggcattca gcgattgaaa
     4021 tgcgtcgata tgctatgcgc tttatccatc atattggtgg tctgcctgat ttcacttcat
     4081 taaaatttaa taaatataat caatatgatt ctatggtgaa accaatcatc agttatttag
     4141 agtctcataa tgtagatgtt caatttgata gcaaggtaac taatatctcc gtagacttta
     4201 agaacggtca gaagcttgca aaagccattc atctcacagt tggtggtgaa gcaaaaacaa
     4261 ttgatcttac tccaaatgat tttgtttttg taacgaatgg ttctatcact gaaagtacga
     4321 actatggtag tcatgatact gtagctaaac ctaataccga tttgggaggt tcttggaatc
     4381 tttgggaaaa cttagctgct caatctgatg aatttggaca tcctaaagtc ttctacaaag
     4441 atattccaaa agagtcatgg tttgtttctg ctactgcaac aataaaagat ccagctattg
     4501 aaccttatat agagcgttta acacatcgtg acctccatga tggtaaagta aataccggag
     4561 gcattgttac agttacagat tctaattgga tgatgagttt tgctatccat cgccaaccgc
     4621 atttcaaaga gcaaaaggaa aatgaaacaa ttgtttggat ttatggactc tattcaaatg
     4681 tggaaggtaa ttatattaaa aaaccaattg aagaatgtac tggtcgagaa atcacagaag
     4741 agtggttata tcacttaggt gttcctgaaa tgaagattca tgatttatct gataaacaat
     4801 atgtaagtac agttcctgtt tatatgcctt acattactag ttatttcatg cctcgagtta
     4861 aaggtgaccg accagatgtt atcccacaag gatctgttaa cctagccttc attggtaatt
     4921 ttgcagaatc tccatccaga gatacagtat ttacaacgga atattctatt agaactgcta
     4981 tggaagctgt ttatactttc ttaaatattg aacgtggtgt tccagaagtc tttaattcag
     5041 catttgatat tcgtgtcttg ttgcaatctt tatactatct caatgataag aaatctgttg
     5101 aagatatgga tttaccaata cctgccttaa tgcgtaaagt aggcatgaaa aaaattagag
     5161 gaacttattt agaagaatta cttcgagaag ctcatctttt ataatgtaaa ataaaatcga
     5221 gaaaacttgt ttttctcgat tttttagttt tacaacgact aaaatcacca aagaaaatga
     5281 aattttgtta taatgtagtc acttactgta cgaaaaggaa gaataatttg caagaatact
     5341 ctatcgaaat tactttacaa catccagatg atatgatgtc actctttggc agcaatgagc
     5401 gccatttaaa attaatcgaa gaaaatttag atgttatcat tcatgctaga actgaacgtg
     5461 ttcaggtatt aggtgactct gaagaagcag tagaaaccgc tcggttaacc attgaagcat
     5521 tattagtgct tgttaaccgc gggatgactg tgaatacttc agatgttgta acagccttat
     5581 ccatggcaca aaatggttca attgataaat tcgttgccct ttatgaggaa gaaattatta
     5641 aggatagcta tggtaagcct atccgtgtta agactttagg gcaaaaaata tacgttgata
     5701 gcgttaaaaa tcatgatgtt gtttttggta ttgggcctgc tggaacggga aaaacttttt
     5761 tagcagtcac tctagcagta acggcactaa agcgtggtca agttaaacga ataatcttaa
     5821 cgagaccagc ggttgaagct ggtgaaagcc ttggtttctt gcctggagat ctcaaagaga
     5881 aagttgatcc ttatttgaga ccagtttatg atgccctata tcaaatttta ggtaaagagc
     5941 aaacaagtcg cttaatggag cgtgaaatca tcgaaattgc tccattggct tacatgcgtg
     6001 gtcgtacttt agatgatgca tttgtcatat tagatgaagc gcaaaacacc actataatgc
     6061 aaatgaaaat gttcctaaca cgtcttggct tcaattctaa aatgattgta aatggcgatg
     6121 ttagccaaat tgacttacca aagaatgtta aatctggttt gattgatgct gttgagaaat
     6181 tacgaaatat caaaaagatt gactttattc atttatcagc aaaggatgtt gtaagacacc
     6241 ctgtcgttgc tgaaataata aatgcttatt cagattcaga aagtagtcac aagctacaat
     6301 ctgataagga ctgatgtttg ccactaggag acgttgttag agagtgtgtc aatatgtaaa
     6361 aaacgcctaa tgctttaggc gtttttatga tggtgtactt caacttaacc tataaagtgt
     6421 tgaaaatttt ctttaagcat gacgtcgaga tgcattatta tatcgtttaa tgtttcttta
     6481 acagtgtgtt cgttggagtt gtgatgacat agtttaatga tattttcttc gaaatgttta
     6541 ggaagcaatg tagcattctt cttagcatag gaaatcattc ttttttcgcc aggatgtgtc
     6601 aacttattaa gtgcaaaaat aatatcaaag taggaatcta agaaagcagt agttctatgg
     6661 tgagtactaa caaagtcttg gcgtttaagg gcttttataa tttgtttatc gtaagaggga
     6721 agcttgccag ttaataagtt acgattttga atgataattt gtttttgtaa ctgactggga
     6781 taagaaactt cgaatctctc tttgagtgat tgtaattgat tttcaggatc atagagtatt
     6841 tggcaattga tgaggttatg ccaaaaacaa gtagtgtatc caattcgaga attgtggtgt
     6901 tcaacgacat cttctaagtc tgataaaaag ttatctatgt tacgatagag aatatcaata
     6961 tcagttccgt cgtttaaagt accattatct tcaagctccc agtaatgatt gttaagttca
     7021 atgtaatgac agtgcttgtt aaggatacgt ttacgactag tgatatcagg agttgctgca
     7081 caataaacat acaaatcata atcggaagat tggtcaaaac tgtcaccaga gcgtgagcct
     7141 ccgagtgcta tagcaacaac atttggaagt ttagagaaat cttgccagag ttttgtgaaa
     7201 tggtccataa atgctctcct aaaaatgtaa aatattgatg tgtctccatt ctacagggct
     7261 atctcttttt agtcaataat taatagtaag ttaataaaag aggtaacggt tctaatattc
     7321 aagaagtgta tatgagtgat ttgaaaagga gatattttat gctacaatac gtacatatta
     7381 gtttaaacaa ggtgatagaa tgaaacaaga ttatatttcc tacattaggt caaaagttgg
     7441 acatgagact atctttttaa catattctgg tggtattttg acagatggta agggacgtgt
     7501 cttactccaa ttaagagctg ataagaattc ttggggaatt attggtggat gtatggagtt
     7561 aggtgagtca tcagtagata cattgaaaag agaatttttc gaagaaactg gattgagagt
     7621 ggaaccaatc agactgttaa atgtttatac taactttcaa gactcttatc ccaatggcga
     7681 taaagcacaa accgtcggtt ttatttatga agtaagctgt ccaaaaccag ttaatataga
     7741 aggttttcat aatgaggaaa cattgcaatt ggactatttt tctaaagaag acgtcaaaaa
     7801 tattacgatc gtaaatgaac aacaccaatt aattttagat gaatattttt cacaaacatt
     7861 ccaaatgggg cgttaaaagg atgggaaata ctaggtgtga ctttaaagaa cttctaactt
     7921 ttattgatat gttgttaggc attcttgtag aaacaaatgt taagaaaata aggagttctc
     7981 atttgtatat tttataatta ttaaaaagaa aaacagtagc ttgcaataat ttagtgactg
     8041 tttttttaga aagcataaga agtacagaaa aagaaaatta tggaatattg gatggtgggg
     8101 atatgcttta cttacggtat tggaatgata tacttaaaga aatccagttt tagaaatcca
     8161 aatttctaag taaaatagca taaaggagag tagatgaatg aagataacat tacacggcgt
     8221 tgctgaaacg ttattaatca ccttatatat tagggctaaa gatgcgatgg ctaaacatcc
     8281 gatacttaat gatcaaaaat ccttagcaat tgttgaacag atagaatatg atttggataa
     8341 attcgataat tcagaagctt ctttttatgc aacattagct agaattcgcg ttatggatag
     8401 agaaatcaaa aaatttatta gagaaaatcc aaatagtcaa atcctttcaa ttggttgtgg
     8461 acttgataca aggtttgaaa gagtcgataa tggacaaatt aggtggtata accttgattt
     8521 gccagaggtt atggagataa gaaaattatt ttttgaagag catgaaagag ttactaatat
     8581 agcaaaatca gccctagatg aaacttggac acgggaggta aatccccaaa atgccccttt
     8641 tctaatcgtg tcagaaggtg ttttaatgtt tctaaaagaa gatgacgtag agacttttct
     8701 tcatatcctg acaaattcat ttagccaatt tatggcacaa tttgatttgt gtcataagga
     8761 aatgattaat aaaggaaagc aacatgatac agtaaagtat atggatacag aatttcagtt
     8821 tggtatcaca gatggtcatg agattgtgga tttagaccct aaattaaagc aaataaatct
     8881 gattaacttt acagatgaga tgagcaaatt tgagttaggc acacttcgct ctttacttcc
     8941 aacaattcgt aaatttaata attgtttagg tgtgtacgaa tataaagcat ctgagaaaaa
     9001 gtaacaaaaa tctatttata taaagagaca ttctaaatgt aaatttgtca taatcgttat
     9061 agcatttgta gctttgttta tttaggaatt agttttacgt aagttaggcg ctctaaagtt
     9121 tgtattttga agtaaatact aattcataag tcagaatttt attgaaatgg attataagtg
     9181 tgtaatagtg attattagct ttctctccct tttttaatac tcttatggta taatgatgtg
     9241 aaagaatagc ttgaaggaag gaagagaacg cacttttatt ttgagaacta gttttagata
     9301 aaagtgaaaa acttaggaaa gtagatgtgt cgattattta gaattatgca ttacacttta
     9361 tcttttctaa acgttttggg tcgttcttac tatataagat gtacgttgaa atgattgatg
     9421 aaactggaca agtatcagaa gatatcaaaa agcaaactct tgatttgtta gagtttgcag
     9481 cacaaaaaac aggtaaagag aataaagaaa tggctgtaac gtttgtcact aatgaacgta
     9541 gccatgaatt gaatttagag tatcgagata ccgatcgtcc gactgatgtt atcagtttag
     9601 aatataagcc tgaggttgat atttcttttg atgaagaaga tctggctgaa aatcctgaac
     9661 ttgctgagat gctagaagat tttgattctt atattggtga actttttatt tctattgata
     9721 aagctaaaga acaagcagaa gagtacggtc actcttatga acgtgaaatg ggttttttag
     9781 ctgtacatgg ttttttgcat attaacggtt atgatcatta tacacccgaa gaagaaaaag
     9841 agatgttcag cttacaggaa gaaattttaa ctgcttatgg acttaaacga caataatcaa
     9901 aaaaaatgga aaaatcgcac cttaacatca agcatggaat ttgctgttac agggattttt
     9961 acagctttta aagaagaaag aaatatgcgt aagcatttgg tttctgccat tttagtaata
    10021 cttgctggtc ttacttttca agtatctatg gtggagtggt tatttctttt attgagtatt
    10081 tttttggtaa ttacttttga aattatcaat tcagctattg aaaatgtagt tgatttggca
    10141 agtaattacc atttctcgat gctggctaaa aatgctaaag atatggcagc aggagcggtt
    10201 ttagttgttt ccttatttgc cgtattggtg ggattgatta tttttatacc caagatactt
    10261 gctttacttt tatgatgaga agtattacga ataaattgaa tggagaaaga aaagaaatga
    10321 catttaagtc aggatttgta gcaattttag gtcgtccaaa tgttggaaaa tcaaccttcc
    10381 taaaccatgt tatgggacaa aaaattgcta tcatgagtga taaagctcaa acaacacgta
    10441 ataaaattat ggggatttat acaacagaaa ctgagcaaat tgttttcatt gataccccag
    10501 gaattcataa accaaagacc gcccttggtg attttatggt tgaatctgct tatagtactt
    10561 tacgtgaagt tgaaacagtt cttttcatgg ttcctgccga tgaaaagcga ggcaaaggcg
    10621 atgatatgat tattgaacgt cttaaggctg ccaagattcc tgttatttta gtaatcaaca
    10681 aaattgataa ggttcatcca gaccaacttt tggaacagat tgatgacttc cgtagtcaaa
    10741 tggattttaa agaagtagtg ccaatttctg cccttcaagg aaataatgtg ccaaccctaa
    10801 tcaagttatt gactgataat cttgaagaag gtttccaata tttcccagaa gatcagatta
    10861 ctgatcatcc ggaacgattt ttagtatcag aaatggttcg tgagaaggta cttcatttaa
    10921 cgcaacaaga ggttcctcac tcagtagcgg ttgttgtcga atccatgaaa cgtgatgaag
    10981 aaactgataa agtgcatatt cgtgcaacta tcatggttga acgtgatagt caaaaaggca
    11041 ttatcattgg aaaacaaggt gctatgctca agaaaatagg aaaaatggcg cgccgtgata
    11101 ttgaacttat gttaggtgat aaagtttacc tagagacttg ggtaaaagtc aagaaaaact
    11161 ggcgtgataa aaagcttgat ttagctgatt ttggctataa tgaaaaagag tattaatgag
    11221 tttattttga gatccgcaag ttagtgggga agagttagat aagttcaccg tgagacttga
    11281 ttcgcacaga aaaagcaatt cacgaggata aaaccaacta gtcataattt tgagattata
    11341 ttcacaaatt aattaaaggg agccaaacat gcttgttggc cccttcttaa ataaaggaga
    11401 gcacatgact caagattata tttgctacct ataaagtaga ggaggtgagg atatgcataa
    11461 tatattaaga tttttaggca ttgttattat ttctgcagtg attctattta gtataggtag
    11521 tttctacgat ttatcattaa tgaaaaatat actacttatc tgttggagtt ttctctttga
    11581 tctattggtt tttgtcttca aacaaagaca aacgacagaa gtactcacat ggtatcaagt
    11641 tgttaagcaa ttttggttat ttattaagtg tactattctt attcctatct tagttgcttt
    11701 tattataatg aaaggatgtt tgacaagtat atcagatata ttaatttact tttatttaca
    11761 ccttgtcgtt gtttattata cgataggaat gatttt
//
LOCUS       DATRNX010000057        11628 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRNX010000057 DATRNX010000000
VERSION     DATRNX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            232..537
                     /locus_tag="VBC81_001256"
     CDS             232..537
                     /locus_tag="VBC81_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6912300.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            537..1385
                     /locus_tag="VBC81_001257"
     CDS             537..1385
                     /locus_tag="VBC81_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6912301.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            1382..2104
                     /gene="fabG"
                     /locus_tag="VBC81_001258"
     CDS             1382..2104
                     /gene="fabG"
                     /locus_tag="VBC81_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6912302.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            2097..2402
                     /locus_tag="VBC81_001259"
     CDS             2097..2402
                     /locus_tag="VBC81_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6912303.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            2386..2862
                     /gene="fabZ"
                     /locus_tag="VBC81_001260"
     CDS             2386..2862
                     /gene="fabZ"
                     /locus_tag="VBC81_001260"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6912304.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            2852..3781
                     /locus_tag="VBC81_001261"
     CDS             2852..3781
                     /locus_tag="VBC81_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912305.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            3774..4652
                     /locus_tag="VBC81_001262"
     CDS             3774..4652
                     /locus_tag="VBC81_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912306.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            4649..6652
                     /locus_tag="VBC81_001263"
     CDS             4649..6652
                     /locus_tag="VBC81_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6912307.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            6649..7602
                     /locus_tag="VBC81_001264"
     CDS             6649..7602
                     /locus_tag="VBC81_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6912308.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            7599..9794
                     /locus_tag="VBC81_001265"
     CDS             7599..9794
                     /locus_tag="VBC81_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6912309.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            9799..11010
                     /locus_tag="VBC81_001266"
     CDS             9799..11010
                     /locus_tag="VBC81_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6912310.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            10979..11554
                     /locus_tag="VBC81_001267"
     CDS             10979..11554
                     /locus_tag="VBC81_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6912311.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
BASE COUNT         3982 a         1644 c         2238 g         3764 t
ORIGIN      
        1 attttaaatt taaactaatg tttatagcag tgttttactc atgattttgt gaattcaaca
       61 cttaagtcat gacctcattt taaagagttt cagacgacac agaaaggaag aaaacataag
      121 aaaggcaatg cacggcttgt ccatagtagt gtttgaagta gatgtttaga aatattttat
      181 tttccaacag tactgatatc agagcgaaga tgattagttg aaggtgataa tatgggacgt
      241 tctaatattt tgagtaggtg tgaacaatgt tctgataatc aatgtggctt gtataaaacg
      301 cggcttaaag acaagttact attagtagtc caactggttg gaaatattag gtattctgag
      361 tttcttacgg aaggtggtgt atatcataag ggagaaacct tgttggaagt tataacctta
      421 ggaatttctt atgaatataa aagttatggg gatatcaaag taatcaagaa gtactgtgct
      481 gatggcagtg tcgttgatta taacatgcca atactattaa tggaaaggat tgaataatga
      541 aaacagtaat aatatatgga ggacagggta atgttcagtc aaaacacctt tgtcaactag
      601 ttaaaactcc aaaaggtgaa attctttttt caaagttaca agcaactgat ttagaatcat
      661 atcagttaat gatggatgtc attaaccaca aggtaagttt agacaatgtt catgcgtcta
      721 tgttagcaaa ttttttatat aatgattggc taacaaacga tgaaaaaata gtatctggga
      781 taggattttc agctcatagc gccggtattt tcaatgtgtt atatgctagt ggtagtacta
      841 gttttgaaaa cattattcac tttattaaac gtcgtgccca acttatagaa aattgtcatg
      901 tgtctgaaga gctttggctt ttgatggttg aaaacattga tatgtttagt aaggatgttt
      961 taaaagaaca gtctgagaaa gtaaaattag ctattattac tgatgataga gctggtgtaa
     1021 ttgctatgga tcaagtcaac cgttctttct ttgatgacgt gatgactaac aaaggttatt
     1081 tttataaatt aaagcagtta ggcgtaaaag caccatatca tactgatttt ttaaatgaat
     1141 ctaaagagca gtataaacaa ttgattgaaa gcctagatat ttcgcaaaat gatgactatg
     1201 actatatttt tcattgtgat caattggtag atgaaatttt atatcagtgg accaatctct
     1261 ttaactggcg tgcgattaag gaaactatca tagaaaagga tgcggagatt attgatattt
     1321 ctcctaatag gtttatttct agtcagttgc aaaaaatgaa aaagaggggg aggaaacagt
     1381 tatgaaagta gcaatagtta ctggtggtac cagaggaatt ggaagggcta ttacaaaaga
     1441 gttatacaaa gaaggttaca aagtcattgc tatttataat agcaatgatg ctaaggcaag
     1501 agctttacaa gaggagttac caaaattaga cgtttataaa tgcaatattt cagatgcaaa
     1561 agcagtacaa aaattggtga ctaaaatttt tagagaatat ggcggtattg attgtttagt
     1621 taataatgct ggtattgtta gagatggctt tttcttgatg atgtctaagg aaaaatggat
     1681 ggatgtcatt aacataaata ttatggggct agttaacatg agtaaagccg tgttaaaaat
     1741 catgaaggct aagcgtatac agggaaaggt tatcaatatt tcttcaacta gtggtatagc
     1801 agggcagata gggcaagcga actattcagc aacgaaaggc gctatcattt ccattactaa
     1861 aactctagcg aaagaatttg catcagatgg tattacgatt aattgtgtaa gtccaggatt
     1921 cattgaaaca gatatgacaa atgaattgca aaataaagaa gagctaaaag agcatcttat
     1981 tcctctaaaa aggtttggac aacctgaaga ggtggcttgg ctcgtaagct ttttagcaag
     2041 tgagaaggca aactatatta caggaaaaaa tattgtgatt gatggaggaa tgattaatga
     2101 ttaactgggc tgaagaacgt aaaataataa aaacacgtca agaagtagtt gatgaaatta
     2161 aaggtatttt gattgactct ttaatgttgg atattgataa agatctgatt atgaatgatc
     2221 aaccattatt tggtcgtgga ttagagctag actctattga tgccctagaa ctgtcaatag
     2281 ggatttcaac aacgtttggt gttgaactaa atgatgatga tatttcaata ttatcatcag
     2341 taaatagact agcagatttt gtgattgaaa atagtgagga tatagatgac caagctaagt
     2401 agtgaagaaa ttataaaaat gttacctcat cgctatccgt ttctaatggt ggatagagtg
     2461 cagagttata aaaaaggtat cattgaatgt cgtaagaata ttactatcaa cgaaccttac
     2521 tttcaaggac attttccaca acgtcctata gttccgggtg ttttgatgat tgagatggca
     2581 gctcaatctg cagctttact ttatatttta gatgcacttg atggtagcct cccctctctt
     2641 gatgaattaa cagcagagaa aatagcagaa aaagtaggtt atttagccag tgtgaagaat
     2701 ttcaaattca agaaaattgt tactccagga gaccagctaa acattacatg taaatctcaa
     2761 tcaaaattgg ggcatctttt agaaattcaa gtcgttatta gagatgagaa taaaaaagag
     2821 gttgcctcag gaaggatgtt ggtatcagaa aatggaaatt aaactcaaaa atattggaaa
     2881 aaaatatggt tcttttgaag cattgagaga cattaatctc atctttgaag agggaaaatt
     2941 ttatggcctt cttggaccta atggagctgg taaaacaact ttatttaacc ttttaattca
     3001 aaattttaag caaacgtcag gcgatattaa gtgggaggtg gatggaaaac ctctatctat
     3061 taaagatttt tatcgtcaca ttggtattgt cttccaaagt aatcgcttag atgataattt
     3121 aacggttgag gaaaatctaa tttctagagg tgctttgtac ggtttatcta aatcacaagt
     3181 acgtaatcgc cttaaagatc ttcaaacgta tctagatatc acagcaataa aaaaacaaaa
     3241 atatggcagt ttatcaggag gtcaaaaacg aaaagttgat attgcacgcg ccttgctgcc
     3301 acaaccttct ctattattat tagatgaacc aacgactggt ttagaccctc aatctcgtcg
     3361 cgatttatgg gatgctatcg cacaactaaa tcaacaatcg cagatgacag ttgtactaat
     3421 tactcattat cttgaagaaa tgtctgcttg tgatgtttta aatgttctta ttgaaggaaa
     3481 catttattat tcgggagata ttaaaagctt tattgagcaa cattccacaa caaacttgaa
     3541 tgtggtattg aaaccagaaa agtcattaga ccaactttcg atagctgatt ttgttaataa
     3601 atgtcaagtc ctttcagaaa gggagatagt atttaaggat atttctgttg aggaaatgat
     3661 gcaggttatc tcagacaatc aagataaatc tattattgag acatttaatg ttgaatattc
     3721 gaacttagag gcggcgtatc tcaacttgct tagagaaaaa gaaaggaaaa acaatgtttg
     3781 aactaatttc tagaaatcgt aaagtttaca cgcgagatcg gttagcgttt ttcatgtctt
     3841 ttttatcagt tattatctta attttggtct atcagatttt cttgggaaaa attcaattag
     3901 atgctataaa agcagcattg aggagtaata cagtatccag tgatactatt cacatggtta
     3961 attattggct cattgcaggt ttgacgacta ttatttcaat gactagtaca ttaggtgcct
     4021 ttggagttat ggtgtccgac aaagaaaaaa aattaaatga agattttaag gtcagtccta
     4081 tatcaaatat caaaattgaa ttgtcctatg ctatatttgc cgttttattt ggtattgtca
     4141 tgacgatgtt ctcttgcatc tttgctatcg gtatttttaa cggctttaat tccttgctag
     4201 agtattcagc tatggattat ttaactattt tagggatcgt ttccttaggg actgttttat
     4261 ctgcggcgat gattttacct attttagcct ttattcatac tagttcagct tttacgactc
     4321 ttagcacaat tgtagggact tttattggtt ttatttcagg agtatatctc tcaattggct
     4381 cggtaggaaa agcattacaa caggttatga catggtttcc attgactcaa atcaattctc
     4441 ttttgaaaca ggtcttaatg aagggttcta ttgcgaaggt atttgacaaa gccaacgaag
     4501 ccactgtctc taactataaa gaatcatatg gtgttgtttt gcgtaatgct gatggagaaa
     4561 ggctgagtaa tcactttatg ttgatttata tcattgccct cattcttatt ttattggcaa
     4621 ttcattttat tattaaaaag gttaaaaaat gaaagatgat aataaattaa agatttctga
     4681 agcttcctta gaagattatt ctgaagtggt tcatttattt aataggaatc atgtttatca
     4741 atttccagac ggtaggcctt taactgttga tgaccttgat ttaacgttaa aagttaaaga
     4801 agtgacacat ttatttttac tgaaaaatca tggtgttttg attggaacat cagctttctt
     4861 taaatttatt acctatggtt gtttagattg gaatagtagc tttagtggtt ttcttttaat
     4921 tgattcgaaa agtcgtagtg gacaggcaat cacttatcta tataaaacta tcttaaagaa
     4981 gataacaaaa ctgaagtttt ctaatatcta taccgaaatc agtaactaca ataaaccctc
     5041 cttagcttta tcaaaattga atggcttcaa agagtatgat aagacatatg aagacatact
     5101 gcattgtcga tcattgcgta gtcacctccc taagatattg aatacgtttc gtatttcgaa
     5161 ttattatggt aaaacatacg acatatcaac ttttcaaatt atggaggaaa ttgaaaatcc
     5221 cttggaagag gagacagaaa ttaggaccaa agtctctgat gaagaaatat tatttaaagc
     5281 agaagatagt gcttcacttc cttattattt aaaaatgagc ttgtttcaaa tggaaattgc
     5341 taagttagat aaccgttatg ttttacaagt tgacttttta tcggaacaag taaagagggt
     5401 tcgggttaaa actggaaaat atcacttggc aaacttaacg agggctcacc ctagcctaac
     5461 tttaagtcgt tttgccaatt attattacat tcaagcaact gtggaaaccc tttatggtaa
     5521 tatcgatgtt caactggaac gtcgaaaaaa gcattataga gatgcaacta tttgtcttaa
     5581 gagaactttt caaggttatg atttacttat ctctccaaat ggcagcctta tttttgaaaa
     5641 gcagaaaaga aagatacttg aagatagttt tttgattttt agccaacctt tggacaagaa
     5701 attagttgta aaagaagaag aaaatcacat tacaatcaaa tgtttctatc aaggtgcttt
     5761 aattgagaaa atcatgacat ttacaagtga cgaagaaatc acttgtgttt ataaatgtaa
     5821 tcaaaaggct aaagagatgt tcccaaaatt attgaaacag actttcaagt tacactgcca
     5881 agagcagttg attagagata gtgaaggtta tcttgtgaat gttccaggaa gttacccgat
     5941 tgagcatgat gattttcttc gagcagataa gtttgaagat agacaattcc attattacct
     6001 cccgaatgaa gataaaatga tttcctattc tcctcctggc aaagccagca atcaaatgca
     6061 atttagaccc ttatgtctta ttgatacaga tagcctttct tttccactga cttatcattt
     6121 taggatttca caagcatcat cggaggaggc gttaatcaat ctaaaaaaac agccaatatg
     6181 ggacagcaac taccaagcgt cagcaactga ccttttaaaa catatctcta acctaacatt
     6241 agaagaagag aaggattatg gcatcaagag aatgatagct aacagaaagc attatcctag
     6301 tcacaaactg gttttagcct ataatcagat tgttttgcct aagaatgaaa ttccaagaga
     6361 cagtgaatta tattctattt cttttgatta tcgaattaga ggaaagtttg tccaaatccg
     6421 ccaaggggaa catgtcaaat atgataataa atcctatgtt ttggaaaata gtcaacaatt
     6481 agtattgtat gtagctagcg atgataaata tatactaatt tctgctaaaa atggaatatt
     6541 ttattcttac aaagagaaca atcatttaaa aataaggtgc atgtttaaaa ggaactcacc
     6601 ttatgctaca aatgtgagca ttacagaata tagaaagtgt gaagaaaaat gaatactatt
     6661 tataatacat tgagaacaga taaaggttat aaagtttatg aggggtattt atatgaaatt
     6721 actggtgaag aatgtgaaga agccttagac cttgtgattc ctaagaatat tgtatttgca
     6781 gatacagata cttgtggcta cactttttta ctcaatgaag atggaacagt ttatgatgat
     6841 gtgactttct acaaatttga tgataaatat tggttggcta gtcataaagc tttggattct
     6901 tatttagaca acatcaattt tgactatacc gtaacagata tttctgacga gtataaaatg
     6961 ctgcaaattg aaggaagata ttcgggagaa attgctcagt cattttatga atatgatatt
     7021 tcaacactta attttcgtac tttgatagag atgacttata aaggtgagaa aggttatctt
     7081 gctagatttg gtttttctgg agaatttggc tatcaatttt tcctaccatc ttctattttt
     7141 gctacttttg tttcggatgt ctgtgaaggt atagcagagt atggggatga acttgataga
     7201 tatttaaggt ttgaagtggg acaacccatt actgatattt atcaacaaga agaatattct
     7261 ttatatgaaa taggttattc ttggaatcta gatttcacaa aggaagaatt tagaggtcgc
     7321 gatagcttgt tagagcacat cagatcagca acagttaaaa gtgttggatt ctcaacgaag
     7381 gaaaaactcg cttcaggaac accagtgcta tttgatgacc aaattgttgg aaagattttt
     7441 tggatagcag acgagaaaga ctcttcggaa aattacctag gtttgatgat tgttaaccaa
     7501 acatatgctc attcaggagt tacttttgta acagaagatg gccaaatttt gaaaacacaa
     7561 tcaagccctt attgtatccc agaaagttgg aacaaagaat gagcgtatat gttagtggaa
     7621 taggaattat ttcttctttg ggaaagaatt atagcgagca taaacagcat ctcttcgact
     7681 taaaagaagg aatttctaaa catttatata aaaatcacga ctctatttta gaatcttata
     7741 caggaagcat aactagtgac ccagaggttc ctgagcaata caaagatgag acacgtaatt
     7801 ttaaatttgc ttttaccgct tttgaagagg ctcttgcttc ttcaggtgtt aatttaaaag
     7861 cttatcataa tattgctgtg tgtttaggga cctcacttgg gggaaagagt gctggtcaaa
     7921 atgccttgta tcaatttgaa gaaggagagc gtcaagtaga tgctagttta ttagaaaaag
     7981 catctgttta ccatattgct gatgaattga tggcttatca tgatattgtg ggagcttcgt
     8041 atgttatttc aaccgcctgt tctgcaagta ataatgccgt aatattagga acacaattac
     8101 ttcaagatgg cgattgtgat ttagctattt gtggtggctg tgatgagtta agtgatattt
     8161 ctttagcagg cttcacatca ctaggagcta ttaatacaga aatggcatgt cagccctatt
     8221 cttctggaaa aggaatcaat ttgggtgagg gcgctggttt tgttgttctt gtcaaagatc
     8281 agtccttagc taaatatgga aaaattatcg gtggtcttat tacttcagat ggttatcata
     8341 taacagcacc taagccaaca ggtgaagggg cggcacagat tgcaaagcag ctagtgactc
     8401 aagcaggtat tgactacagt gagattgact atattaacgg tcacggtaca ggtactcaag
     8461 ctaatgataa aatggaaaaa aatatgtatg gtaagttttt cccgacaacg acattgatca
     8521 gcagtaccaa ggggcaaacg ggtcatactc taggggctgc aggtattatc gaattgatta
     8581 attgtttagc ggcaatagag gaacagactg taccagcaac taaaaatgag attgggatag
     8641 aaggttttcc agaaaatttt gtctatcatc aaaagagaga atacccaata agaaatgctt
     8701 taaatttttc gtttgctttt ggtggaaata atagtggtgt cttattgtca tctttagatt
     8761 cacctctaga aacattacct gctagagaaa atcttaaaat ggctatctta tcatctgttg
     8821 cttccatttc taagaatgaa tcactttcta taacctatga aaaagttgct agtaatttca
     8881 acgactttga agcattacgc tttaaagggg ctagaccacc caaaactgtc aacccagcac
     8941 aatttaggaa aatggatgat ttttccaaaa tggttgccgt aacaacagct caagcactaa
     9001 tagaaagcaa tattaatcta aaaaaacaag atacttcaaa agtaggaatt gtatttacaa
     9061 cactttctgg accagttgag gttgttgaag gtattgaaaa gcaaatcaca acagaaggat
     9121 atgcacatgt ttctgcttca cgattcccgt ttacagtaat gaatgcagca gctggtatgc
     9181 tttctatcat ttttaaaata acaggtcctt tatctgtcat ttcgacaaat agtggagcgc
     9241 ttgatggtat acaatatgcc aaggaaatga tgcgtaacga taatctagac tatgtgattc
     9301 ttgtttctgc taatcagtgg acagacatga gttttatgtg gtggcaacaa ttaaactatg
     9361 atagtcaaat gtttgtcggt tctgattatt gttcagcaca agtcctctct cgtcaagcat
     9421 tggataattc tcctataata ttaggtagta aacaattaaa atatagccat aaaacattca
     9481 cagatgtgat gactattttt gatgctgcgc ttcaaaattt attatcagac ttaggactaa
     9541 ccataaaaga tatcaaaggt ttcgtttgga atgagcggaa gaaggcagtt agttcagatt
     9601 atgatttctt agcgaacttg tctgagtatt ataatatgcc aaaccttgct tctggtcagt
     9661 ttggattttc atctaatggt gctggtgaag aactggacta tactgttaat gaaagtatag
     9721 aaaagggcta ttatttagtc ctatcttatt cgatcttcgg tggtatctct tttgctatta
     9781 ttgaaaaaag gtgataaaat gagtaaattt ttatttgctc ctttcatgat gaatttagga
     9841 gagagccaaa gattatcaaa actggctaat tgtttatatg aacaaggaca tgatattcat
     9901 atccttggag acaattatta tccattttta tttaataatg atgcttatcg ctatcaccac
     9961 tgtgtagaag ataaatctgt ttataatagt aagcgatatg atgctttctt ttctctttca
    10021 acagacttta attttttatc agaagaagaa attgaacgca tctgttctat tgaaagagac
    10081 ttgttacgcc aagaaaaatt tgatgctgta ttgacaggtt accgtctatc tattgttaca
    10141 agttgtcgtt tagaaagcat accattaatt tggattattt caggggcaac gcatatcagt
    10201 gaaattgttg aaaactcaga aggaatattg cctaatggga agataagtaa agctagtaag
    10261 cctcaaacaa aggattttat caaaagagta atcactactt attcaacaaa tgttaaaacg
    10321 tggaataact atattaaaaa atatggtggc aaacctttta ataatgcttt agaattattt
    10381 actggtgatc taaacttggt aacagattac tctttgtttt atgaatttga taaagattcg
    10441 tcctataaaa cgataggacc tattttgata gataatgtag gtttttcaaa atgcagccaa
    10501 attaatcaag acaataagac tgtactgctc agctttggta cttcatttaa acgagattgg
    10561 gtggaatctt ttttaaagac attaccaagg cattatcatt atttactgac aagctgtggt
    10621 gaggctataa acattccagg aagttatatt gagatagttg attttattga ttttaaaacc
    10681 atagataaag agatttattt cgctattata cacggtgggc aaggaacagt ttatgcgatg
    10741 gctgcacaag gtattccctt tataggtatc cctttcttta acgaacaatt ttggaatatt
    10801 aagaagttca gtaaagaaaa gtgcgcttat ttggtaaaga aacctgatgt aagagagata
    10861 cgtaaagcaa ttagctgtct tgaaagagac tatgatatct ataaaagtta tatgataaga
    10921 ctaagccaag gaattaaaga agaagcagag gcatctttac aaaaagcgga ggcagaaatt
    10981 gaatccttta tcagaaaaag acaaagttaa ctatattatg atttataaaa ataggcagtc
    11041 tacattggag aataatttat ctggcgatcg gttgatgaga tatcaacaag caaatagaca
    11101 ggtaagcaaa gataaactgt taacagggag ttattttgtg taccaatgct taaaaaagtt
    11161 aggttttacc aataagattt gtcaggaatc tttttctagt gttagtagtt atttaattgg
    11221 tttgccaaag gggaaaatta gttactctaa ttctggtgac tatcatattc taacctatgc
    11281 tcccagtggt tcaactgggg ttgatattga gaaatacaaa gatagatcag aacaaaccta
    11341 ccaaaactac ctaggagaat cagttagtag tgatatggat tctaaattat tattttataa
    11401 ggcatggtta caaaaagaaa tttcctataa atgtgggaaa tctatagata ttacctatca
    11461 aacaatgata gatggctata tttatggtta tgcttttgat aatactttta aagtagaagc
    11521 ggtttactta aaagaattat taccagataa ctaactcgca acatgttgtt cctacatatg
    11581 catctataaa aagcactttg aagtatttca agggcttttt ataatacc
//
LOCUS       DATRNX010000058        11247 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRNX010000058 DATRNX010000000
VERSION     DATRNX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            110..820
                     /locus_tag="VBC81_001268"
     CDS             110..820
                     /locus_tag="VBC81_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912312.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            813..2162
                     /gene="vicK"
                     /locus_tag="VBC81_001269"
     CDS             813..2162
                     /gene="vicK"
                     /locus_tag="VBC81_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6912313.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            2166..2975
                     /locus_tag="VBC81_001270"
     CDS             2166..2975
                     /locus_tag="VBC81_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6912314.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            2978..3346
                     /locus_tag="VBC81_001271"
     CDS             2978..3346
                     /locus_tag="VBC81_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6912315.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            3521..4207
                     /locus_tag="VBC81_001272"
     CDS             3521..4207
                     /locus_tag="VBC81_001272"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6912316.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            4215..7754
                     /gene="smc"
                     /locus_tag="VBC81_001273"
     CDS             4215..7754
                     /gene="smc"
                     /locus_tag="VBC81_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6912317.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            7845..8642
                     /locus_tag="VBC81_001274"
     CDS             7845..8642
                     /locus_tag="VBC81_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6912318.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            8642..9466
                     /locus_tag="VBC81_001275"
     CDS             8642..9466
                     /locus_tag="VBC81_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6912319.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            9466..11076
                     /gene="ftsY"
                     /locus_tag="VBC81_001276"
     CDS             9466..11076
                     /gene="ftsY"
                     /locus_tag="VBC81_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6912320.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT         3836 a         1698 c         2367 g         3346 t
ORIGIN      
        1 aattagtgat aatagtggtg ctttaggccc atattatcat attttcggtc acttttattt
       61 ttcagtttat ggatggaagt gggagaccag aaagtaatag aggtaaaata tgaaaaaaat
      121 acttatcgtt gatgatgaaa aaccaatttc agatattatc aaatttaatt taaccaaaga
      181 aggatatgaa acggcgacag ctttcgatgg ccgtgaagct ttagtacaat atgcagaatt
      241 tcaaccagat ttaatcattt tggatttaat gctccctgaa ctagatggtt tagaagtggc
      301 taaggaagtt cgtaaaacca gccatattcc tattattatg ctttccgcaa aagacagtga
      361 gtttgacaaa gtcattggtt tagaaatcgg tgcagatgat tatgttacta aacctttttc
      421 aaatcgtgaa cttttggcac gggtcaaagc acatttacga cgtactgaaa atattgaaac
      481 agctgtagca gaagaaagtg ctcaaaatgc ttcttcagat attactattg gtgagttgca
      541 aattttacca gacgccttta ttgctaagaa acgtggcgaa gaaattgaat taacgcaccg
      601 agaatttgaa ttacttcacc atttagcgac acatatagga caagtcatga cacgcgaaca
      661 tttattggaa acggtttggg gatatgatta ttttggagat gtccgtacag ttgatgtgac
      721 ggtgagacgt ttacgtgaaa agattgaaga tactcctggt cgtccagaat atattttgac
      781 tcgacgtggt gtaggttatt acatgaagtc ttatgaataa tagtgctgca aatattaggt
      841 cgtttgagct agccttactt tttttactag tttttgttgc tgtctatttt gtttatctag
      901 cggttagaga ttttaaaatg tctaaagata tccggctttt gaattggaaa gtcagagatt
      961 tgatagcagg aaattattca gatagtattc taattcaagg tgatgcagat ttggtagaat
     1021 taggggaaag tctaaatgat ttatctgatg tcttccgaat ggcacatgat aacttagaac
     1081 aagaaaagaa tcgcttagcg agtatcttaa cttatatgac tgacggtgtg ctggctacag
     1141 atcgttcagg aaaaatcgtt atgattaatg agacagccca acagcagttc aatctagcat
     1201 atgatgaagc tttatcaatg aatattgttg atatgttagg gtcgggaagt ccatattcgt
     1261 tccaagattt agtctctaag acgccagagg tagttcttaa tagacgtgat gaaaatggtg
     1321 aatttgttac cttacggatt cgatttgctt tgaaccgtcg tgaaagtggt tttatttccg
     1381 gtttagttgc agtatcacac gatgctactg agcaagagaa agaagagcgt gaaagaagac
     1441 tttttgtatc taatgtttct catgaacttc gtacaccttt gacatctgta aaatcttatt
     1501 tagaagcttt agatgaggga gcgctcaatg aagaagttgc tccaagcttc attaaggtat
     1561 ctctagatga aacaaatcgt atgatgcgta tgatatcaga tttattaagt ttatcgcgca
     1621 ttgataatga agtaacgcat ttagatgttg aaatgacgaa ttttacagct ttcatgacct
     1681 caattttgaa tcgatttgat cagattagaa atcaaaaaac agtcacagga aaagtttatg
     1741 aaattgtcag agattatcct cttaagtcaa tttgggtgga aattgataca gataagatga
     1801 ctcaagtgat tgataacatt ttaaataatg cagtcaagta ttcaccagat ggtggtaaga
     1861 ttacagttaa tctacgcaca actaaaacgc agatgatttt atcaatatca gaccaaggct
     1921 taggtattcc caaaaaagat ttacctctca tttttgatcg tttttatcgt gttgataagg
     1981 cgagaagtcg tcaacagggt gggactggac ttggtttgtc aattgcaaaa gaaattgtta
     2041 agcagcataa gggatttatt tgggctaaga gtgagtatgg taaagggtct acttttacaa
     2101 tcgtcttgcc ttatgataaa gatgctgtaa cttatgaaga atgggaggac gttgaagatt
     2161 aaaacatgtc agaaatagga tttaaatata gtatcttagc ttcaggttca actggaaatt
     2221 gtttttatat tgagacaccc caaaaacgtc ttttaattga cgctggttta acaggcaaga
     2281 aagtgactag ccttcttgca gaaattaatc gtaaaccaga agatttagat gctatactag
     2341 ttacccatga acattctgac cacattaaag gtgttggcgt attggctagg aaatatcacc
     2401 ttgatattta tgccaatgag cagacgtgga aagttatgga tgagcgtaac atgttaggca
     2461 aagttgatgt tagtcaaaag catgtttttg gacgcggtaa aaccttaact ttcggggatt
     2521 tagatattga gagctttggt gtaagtcatg atgctgtgga tccccaattt taccgtatga
     2581 tgaaagatga taagagcttt gtgatgttga cagatacagg atatgttagc gaccgaatgg
     2641 caagcttgat tgagaatgca gatggttatt tgatcgagtc aaatcacgac attgagattc
     2701 tacgttcggg ttcttatcct tggacactta agcaacgtat tttatcagat aaaggacatt
     2761 tatctaatga agatggttca gaaaccatga ttcgcactat aggtaaccgt actaaacaca
     2821 tctatttagg acacttgagt aaggaaaata atattaagga acttgctcac atgactatgg
     2881 aaaacaattt aatgagagct gactttggtg tgggcacaga tttttctgtt cacgatactt
     2941 cacctgattc agctacccca ctgacgagga tttaagattg aaaaagattt tttacattag
     3001 tttaggattt attagtttag ggataggcat tgcaggtatt gtcttgccag tagttccaac
     3061 gactccatta gtattgcttt cagctttttg cttttcgaga agctctgaaa aatttgatat
     3121 ttggttgcgt caaactaaag tttataaata ctacgctgca gattttgttg aaagcagaag
     3181 tattgcccca gcgcgtaaaa agtcaatgat ttggcaaatt tatatcttga tggggatttc
     3241 aatatatttt gctcctctaa tgtggctcaa actgggatta ttgattggaa ctattgtagg
     3301 aacttatgtg cttttttatg tagtacctga taaaattgat gaatagttaa caagagacta
     3361 ggtgtgtatc ctagtctttt gttgaatatt caaatatgta attaaggaaa cgttttcttt
     3421 ggaagataag tttaacttcc aaaatgtttg attatttgtc aactgtggta taatggtatt
     3481 tgtttaatat tttatctatg attgaaatca aggagaaaaa atgaaagaat taagatcaaa
     3541 gctggaaaaa gactatggta ttgtttttgc taatcaagaa ttattagata cagcttttac
     3601 ccatacatca tacgcaaatg agcatcgcct cctaaacatt tcacataatg aacgtttgga
     3661 atttttagga gacgctgttc tacaattact tatttcacag tatcttttta caaagtaccc
     3721 tcaaaaggca gaaggtgatt tatcaaaatt acgttcaatg attgttcgtg aggaaagtct
     3781 tgcaggtttt tcacgtctat gtggctttga tcactatatt aaacttggaa aaggagaaga
     3841 aaaatctggt ggacgtaatc gtgacactat tttaggagat ttatttgagg cttttttagg
     3901 agctttatta ttggataaag gtgttgaagt ggttcatgcc tttgttaaca aggtaatgat
     3961 ccctcacgtt gaaaaaggga cctatgagcg tgttaaagat tacaagacaa gcttgcaaga
     4021 attacttcaa tctcatgggg atgtaaaaat agattatcaa gttactaatg aaagtggtcc
     4081 agcacatgca aaggaatttg aggtgactgt atctgtcaac caagaaaatt tgagccaagg
     4141 aattggacga tcaaagaaag ctgctgaaca agacgctgcc aaaaatgctt tagcaacgtt
     4201 acaatgagga aatcatgttt ttaaaagaaa ttgaaatgca aggctttaag tcctttgcgg
     4261 ataaaactaa ggtagaattt gatcaaggag ttactgctgt tgtaggtcca aatggatcag
     4321 gtaaaagtaa tatcacagag agtttacgtt gggcattggg agaatcaagc gcaaaaagtt
     4381 tacgaggagg taagatgcca gacgtcatat ttgctggtac agaaaatcgt aagcccctta
     4441 attatgcgca agtgagtgtt actttagata acagcgacca ctttatcgaa aatatagctg
     4501 acgaagttcg agttgaacgt cgtatttttc gaaacggaga tagtgaatat ttaatagatg
     4561 gacgcaaagt tcgcttacgt gatattcatg atttatttat ggatactgga ttagggagag
     4621 attctttttc tattatttcc caaggacgcg ttgaagctat ttttaatagt aaaccagaag
     4681 agcgccgtgc catatttgaa gaagccgctg gtgttctgaa atacaaaaca cgtaagaaag
     4741 aaacacaatc caaattagaa caaactcaag ggaacttaga tcgcttagag gatattattt
     4801 atgaattgga catgcaagtc caaccattag aaaaacaagc aagtattgct aagcgttttt
     4861 tagtattaga tgaggaacga caaggactcc acttatctat tttgattgaa gatattttac
     4921 aacatcaatc agatttaact actgttgagg aaaagctatt gacagtaaga gaagagttag
     4981 caacttatta ccaacaacgt cagtcccttg aagatgaaaa tcaatcgtta aaacagaaac
     5041 gtcaccattt atctgaagaa attgaggtta aacagttagc tcttttagac gttacaaagt
     5101 taaagtcaga tttggaacgc caaattgatc tgattcgttt agagtcaagc caaaaggctg
     5161 aaaagaaaga agaggcaggt caacgcttag cagagttaga agttaaagct aaagattgta
     5221 atgaccagat tacccaaaaa aatatagagt taactactct gtcggaaaaa atagcccaaa
     5281 taagatcaga aattgttagt atagaaagtt ccttagagcg tttttcaact aatccggatc
     5341 aggttattga aaaacttcga gaagactttg ttaccttgat gcaagaagaa gccgacactt
     5401 caaacgcttt aacagcttta ctagctgata tagagaatca aaaacaagct tctcaagcta
     5461 aatctcaaga aatacaagaa gttagcaaaa atttagaagt attaaaaagt aacgcaaagg
     5521 tagcattgga acgatttgag gctgcaaaga agaatgttcg tcagttatta tctcactacc
     5581 aagatttagg tcaaaccttg caaaatctag aaggtgagta taagaatcag cagtcaattt
     5641 tatttgatca tttagatgaa atcaaatcaa agcaagcacg tatttcgagt ttagaatcta
     5701 ttttgaaaaa tcatagtaat ttctatgctg gagtgaagtc agttctacag gctaaggacc
     5761 agcttggagg tatcataggt gctgttagtg agcatcttag ctttgacaaa cattaccaaa
     5821 cagcattgga gatagctttg ggaggtagca gccaacacat cattgttgaa gacgagagtg
     5881 ctgccaaacg ttcaattgct tttttaaaga aaaaccgcca aggtagagcg acatttctgc
     5941 ctttaactac aataaaacca agagaattag cacaacatta tctctcaaaa ttacaaagtt
     6001 ctcaagggtt tttaggaata gctagtgaat tggtaaccta tgatcaacgc ttgtcaaaca
     6061 tttttaaaaa taacttaggg ttaactgcta tttttgatac ggttgataat gctaatgtag
     6121 ctgcaagaca attgaattat caagtacgtt tagtaacctt agatggaaca gagttacgcc
     6181 ctggagggtc gtactcaggg ggagcaaatc gtcaaaataa tactgttttt atcaagcctg
     6241 aattggataa tctaaaaaaa gaattgaaac aggcacaaag taaacagtta attcaagaaa
     6301 aagaagttgc cgcattgtta gagcaactca aagagaaaca agaaacctta gcacaactta
     6361 agaatgatgg agaacaagct cgtcttgagg aacaaagagc tgatatagag tatcaacaac
     6421 tatcagaaaa gcttgctgat ttaaataaac tatataatgg cttgcaatta agcagcgggg
     6481 cgttggaaca aactacttct gaaaatgaaa aaaatcgttt agaaaaagaa cttgaacaat
     6541 ttgctattaa aaaagaagag ttgacaactt caattgctca aattaaggaa gataaagatt
     6601 caattcaaga aaaagtcaat aatctaacaa cattattgtc agaggcacag ctagaagaac
     6661 gtgatttgct aaacgagcag aaatttgaac gtgccaactg tacacgtcta gagattacct
     6721 tgtctgaaat aaaacgtgac attagtaatt tacaaacgtt actaagccat caggacagtc
     6781 aattagacaa agaagagctt ccacgtatag aaaaacagtt gctacaagca aataagagaa
     6841 gagaaaatga cgaagaaaag cttgtgagtc tccgttttga attggaggat tgtgaagctg
     6901 ctttagatga tttagctgct tctctggcta aagaaggaca aaaaaatgaa agccttattc
     6961 gtcagcaagc tcaattagag tcacaatgtg aacaattatc tcaacaattg atgatattct
     7021 cacgtcaatt atcagaagac tatcagatga cattggatga agcgaaggta aaagcaaatg
     7081 tcttggaaga tattttgatg gcgcgtgaac aattgaaatc tctacaagct aaaattaaag
     7141 ctttaggacc tgttaatatt gatgcaattg ctcaatttga agaagttcat gaacgattga
     7201 ccttccttaa cacacagcga gacgacttag ttcatgccaa aaatttatta ctagagacga
     7261 ttacagatat ggatgatgaa gttaagactc gttttaaatc gacatttgaa gccattcgtc
     7321 atagtttcaa agaaaccttt gtacagatgt ttggtggagg ttctgcgaac cttattctga
     7381 cagaaggtga tttattatca gctggggttg atatttcggt tcaacctcct ggtaaaaaaa
     7441 tccagtcttt aaacttgatg tcaggtggtg aaaaagcatt atcagcgtta gcattattat
     7501 ttgccattat ccgtgttaag acaattccat ttgtcatctt ggatgaagtt gaggccgctt
     7561 tagatgaagc aaatgttaag cgatttggcg attatcttaa tcgatttgat aaatcaagtc
     7621 aatttatcgt tgttactcac cgaaaaggga ctatgtctgc tgctgatagt atctatgggg
     7681 taacgatgca ggaatcaggc gtttccaaaa ttgtttctgt aaaattaaaa gaagctcaag
     7741 aaatgaccaa ttagaaaaca gaaggggatc ccttctgttt tttggatttt cagccttatt
     7801 ttatgttaca atagtaaatg taaacccttt agttaggaga ttccatgata aaattagtag
     7861 caaccgatat ggacggtaca ttcttagatg aaaacggtac ctatgataaa aaacgtttag
     7921 caaatgtgct aaaaaaattt aaagaacaag gaattgtctt tacggcagca agtgggcgtt
     7981 ccttattatc acttgaacaa ttatttgctg actttcgtga tcagatggct tttatagcag
     8041 aaaacggaag tgcagcagtc ttatttaatc gcttggcata tgaacagcac ttgagtcgag
     8101 aacaatactt agatattatt gaccatttga gtaagagccc ttatatggag aataatgaat
     8161 atgtcttatc tggcaaggat ggtgcatata ttctaagtga tgccaatcca gattatattg
     8221 aattcatcac tcattattat gataatttgc aaaaagtttc tcactttgaa gatgttgatg
     8281 atattgtttt caaagtaacg gctaacttta ctgaagagac tgttagacaa gctgaggagt
     8341 gggtaaatca agctataccg tatgccacag cagtgacaac aggttttaaa tccattgata
     8401 ttattctaag ctcagttaat aaacgaaatg gtttagagca cctctgtgaa caatacggta
     8461 ttagagcaga agaagtctta tcttttggtg acaatattaa tgatttagaa atgctagagt
     8521 ggtcaggaaa agctatcgca acagaaaatg ctagaccaga agttaaagag atagcagatt
     8581 gtattatagg tcatcacaat gatcaagctg taatggctta tttagaaagt atggtggatt
     8641 aatgttggat attaaaatac ttgccttaga tttagatggc actttattta caacagataa
     8701 gaaagtttca gaagaaaata aagtggcctt aaaagcagct agagaaaaag gtataaaagt
     8761 tgttattaca acagggcgtc ctttaaaagc aattggtaat ttgttagagg acctagagtt
     8821 agtatctgat gaagattaca gcattacttt taacggtggt ttagttcaac aaaatacagg
     8881 gaaaatctta gctaaaactg caatgacacg acaagaagtg gaagatatcc atgaggagtt
     8941 gtatcaagtg ggtttgccaa cagatatctt aagtgaggga acggtatata gtattgctaa
     9001 caagggacac cattctcaat atcatttagc caacccactc ttagaattta tagaagttga
     9061 tgatttagag caagtgccaa aggatgtagt ttataataaa attgttagtg ttattgatgc
     9121 tacatatctt gatcaacaaa ttgctaaact tcctgataga cttaaagttg actatgaaat
     9181 gtttaaatca cgtgatatca ttttagaatt gatgcctaaa ggtgttcata aagcagtcgg
     9241 cttagaacta ctgacaaaac accttgggtt agatagtagt caagttatgg caatgggaga
     9301 tgaagcaaat gatttatcaa tgctagagtg ggcaggctta ggagttgcaa tggctaatgg
     9361 tattccagaa gctaaggcaa cagcaaaagc gacaactatt tgtaataacg atgagtctgg
     9421 agttgccgaa gcagttggga aatatatttt aagtgaggaa aattaatggg attatttgac
     9481 cgcctatttg gacataagaa aaaagataaa gaactagaaa ttgaagcatc agaatctgtt
     9541 gttttagaaa atgaagattc agttattgat aaagaggaaa gcagcaactt ttcgaaggag
     9601 tcaactttga atcgaacctc tgaagtccct gtcgcagaag atgattcttt cttagaatta
     9661 gaaagagata cagcattgtc agaatcacat caaccagtta cctctgaaat tcatccttta
     9721 gagtcagaag atacctctga aattcctgta aaagaagacg atagtttttt agaactagaa
     9781 gatagggcta agacaaaggt ggcagatact tctgaggtag gaaatgtagt tcctgattcc
     9841 acgactcttt ctgacaatgt ttctgctaaa tctgaggcat cttttagtga tgaggagcaa
     9901 tcaagtgata gtcaggcttc agagcagttt tcagaaactc cccttcaaga agagatgtct
     9961 agtggaaaaa ctgaggtaca aacagagtca gaggatacat ctgcggctga tgctttttta
    10021 gcagattact atgctaaacg taaagcaatt gagaaagaaa tctcgtctaa ttcgctgtca
    10081 acagatgagt cagaactttc tgaggcacag gaagttttat cgcaatcaca ggcagacact
    10141 ataaaagctg agagtcaaga ggaaaaatat aatcgtagcc tgaaaaaaac acgcacaggt
    10201 tttagtgcac gccttaatgc tttcctttct aacttccgtc gtgttgacga agagttcttt
    10261 gaagaattag aagaaatgtt aatcttatct gacgttgggg taaatgttgc aacacagtta
    10321 actgaagacc ttcgttatga agcaaaatta gagaatgcca agaaatcaga ggatttaaaa
    10381 cgtgttatcg ttgaaaaatt agttgaaatc tacgaaaaag atggtattta taatgaggct
    10441 atcaatttcc aagaaggttt aaccgtaatg ctttttgtag gtgttaatgg tgttggaaaa
    10501 acgacatcta taggtaagtt agctcatcaa tacaagtctc aagggaaaaa agttatgtta
    10561 gtagcagctg atactttccg tgcaggtgct gtggctcaac tagtagaatg gggacgccgt
    10621 gttgatgtac cagttgtcac tggagaaaaa aaagcagatc cggctagcgt tgtctttgat
    10681 ggcatggaaa aagccgttgc tcaaggcgtt gatgtcttgt taattgatac agcaggacgt
    10741 ttgcaaaata aagaaaacct tatggcagag ttggaaaaaa ttggacgtat tattaagcgt
    10801 gtcgtaccag atgctcccca cgaaactttg cttgctttag atgcatctac aggacaaaat
    10861 gctttaagtc aagctaaaga gttctcgaaa attacaccat taactggcct tattctgact
    10921 aaaattgatg gtactgctaa gggtggtgtg gtactggcta ttcgtcaaga actagatatt
    10981 cctgttaaat tcattggttt cggcgaaaaa atcgatgata ttggtgaatt taattcagaa
    11041 gatttcatgc gtggccttct ggaaggaatt ttataagaaa aaagaacttt catttagaaa
    11101 gttcttttta tagtacgacg ggcatgtcgt acatctgagg tgcaagtcct ctgtgggcac
    11161 ccgctaccgg tgaacccaat agcgactccc aagcttgact atcgtgaggt agcggggaga
    11221 ggaagggata gcgaaatcat ggctcta
//
LOCUS       DATRNX010000059        11057 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRNX010000059 DATRNX010000000
VERSION     DATRNX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(47..223)
                     /locus_tag="VBC81_001277"
     CDS             complement(47..223)
                     /locus_tag="VBC81_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912321.1"
                     /translation="MAYPDKSFKPTPINELIEKFDKEINQFMENQKEVISVNTQTNGL
                     AGGFQYFVTVVYRN"
     gene            complement(252..629)
                     /locus_tag="VBC81_001278"
     CDS             complement(252..629)
                     /locus_tag="VBC81_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6912322.1"
                     /translation="MSKKNAMRKLKEFQRWQRIANSLDLTYNELYQFDIEYHSTRRTY
                     LEISRECALEELGAIKYAINQLSKIDYRQILIECYLISEKLSNQEIMTKLNRSESWYY
                     ETKKRALLEFAEHYRDGILNKIN"
     gene            complement(604..966)
                     /locus_tag="VBC81_001279"
     CDS             complement(604..966)
                     /locus_tag="VBC81_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6912323.1"
                     /translation="MGAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAQQLLCLAKERDKRLAMITSLQDENQIAVIKARYVDDLSWDEIPDKVGCSRNTVF
                     KLHREALEVLDEQEERHA"
     gene            complement(1367..1855)
                     /locus_tag="VBC81_001280"
     CDS             complement(1367..1855)
                     /locus_tag="VBC81_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912324.1"
                     /translation="MLELAIESIIKPMKAQRKTKITGTMDNQPIRIDLDNLVIHYNHQ
                     NLLLETIPGTYGGKRYFFLCPKCERRCRKLFKASHDFACGSCQKVHQATLNRSKTDCC
                     YYWRLAFKECLKVDPKAKHKHGYYSHDDFPKRPKYMRIAKYLYHWKRFHYYMDKGDRH
                     WL"
     gene            complement(1929..2468)
                     /locus_tag="VBC81_001281"
     CDS             complement(1929..2468)
                     /locus_tag="VBC81_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912325.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYHAPVIG
                     VTIKGKGQLVLEYVEDKKLSKVFKRICDSFKDYYNQSEDMTDEELDDMAQENELIKEV
                     EELDDQRVVPLFEITQEEANEQDTLMAFISDHDQLAEHLSMNYQEMSQDDLGAILETI
                     SQAFNHLYDMVVEGQLLVK"
     gene            complement(2470..2643)
                     /locus_tag="VBC81_001282"
     CDS             complement(2470..2643)
                     /locus_tag="VBC81_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912326.1"
                     /translation="MKLQLHTRGGNTITIQGDRTLYNELVKYLLSGQQPNWVACPSAI
                     INLSDIIAITKEK"
     gene            complement(2649..2867)
                     /locus_tag="VBC81_001283"
     CDS             complement(2649..2867)
                     /locus_tag="VBC81_001283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912327.1"
                     /translation="MKIKLFNRKRIETGFNEYMDLPKFRNETNEEFENRVNSFIADKK
                     VIDIKYQEATYGNYEDMSTTTSLLVLYR"
     gene            complement(3142..4767)
                     /locus_tag="VBC81_001284"
     CDS             complement(3142..4767)
                     /locus_tag="VBC81_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6912328.1"
                     /translation="MKSLESEILEARESEDKYFKTFKSVRGQLIKECQSIKEEAYQIA
                     YDEVMADSEHLENVKAGRLTEVQHKELAHQKGQEASEKALPKTPLGVAIMLKQYIHFI
                     RIKPEAQGQKAPLYYYNPDFGIWLEDNELLQDLISTIFPNATEKQAFDTLYKIARQSQ
                     MKEIQGKYTVIGKQLFNNKTGEFEPFNPKIIVTRKIKTAYNPYAKEPTINGWKVTEWL
                     KELFDGDKELYQLAIQIIKASITGQSLEKIFWLYGEGGTGKGTFQQLLINLVGLENVA
                     SLKITDLNKSRFTTSILLGKSLVIGDDVQKDAIIKDTSDMFSLATGDIMTIEDKGKKP
                     YSLRLNMTVVQSSNGLPRMNGDVSAIDRRFRILIFSSRFKDKPNPAIKKDYINRKEVL
                     EYLVKLAIETPSEDINPKQSQTLLGEHQKDINPVLDFVEKTFREDLASEFIPNDFIWY
                     CWKQYQDYFNQSFFKTERGLHRDIKQVLPPYIRTGVRTIPSGRQLHLGFYPKEDTPDY
                     ASLATYSNGRETPEKRKRPKQDRGYWNSKAKHK"
     gene            complement(4787..5635)
                     /locus_tag="VBC81_001285"
     CDS             complement(4787..5635)
                     /locus_tag="VBC81_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEO6912329.1"
                     /translation="MAIYEARGFSSYLYPYKGQLEPFDYIAQFKPLKPPEGINIEEYK
                     RTQAPYCLSGKVTAEKNGGYKRNNASLVYRDLIFLDYDEIETGANLPKIVSKTLWEYS
                     YIIYPTIKHTPEKPRYRLVVKPSDTMDKTTYKQVVKEIANKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNRGLDYPVPKAPNRQVTTTYTSRPRSQRSITMRVIDTLFNGFG
                     DEGGRNVALTKFVGLLFNKWVDCDIETAYELTKIANGVTDNPLPIEELDRTFTSIARA
                     EFRKRG"
     gene            complement(5636..5908)
                     /locus_tag="VBC81_001286"
     CDS             complement(5636..5908)
                     /locus_tag="VBC81_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912330.1"
                     /translation="MSLFSQDYEAKLLEQNLTAFNRFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVNV"
     gene            complement(5911..6240)
                     /locus_tag="VBC81_001287"
     CDS             complement(5911..6240)
                     /locus_tag="VBC81_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912331.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNIPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(6237..6599)
                     /locus_tag="VBC81_001288"
     CDS             complement(6237..6599)
                     /locus_tag="VBC81_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912332.1"
                     /translation="MTTEEYFKKLLEDSEKQPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRDYNKADAVVKFLESIPIEMEYKKGLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            complement(6612..6803)
                     /locus_tag="VBC81_001289"
     CDS             complement(6612..6803)
                     /locus_tag="VBC81_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912333.1"
                     /translation="MQELNLTPTQTLILFIVLGLLGLLLSLSKSLIDIDLPEDTQVPK
                     PRQNANYGAYIQSQNHYYN"
     gene            complement(6803..7135)
                     /locus_tag="VBC81_001290"
     CDS             complement(6803..7135)
                     /locus_tag="VBC81_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912334.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEAYFTRLYNQH
                     IARVIIANVMAEYTTTFRKSATSFEEAWEALDYQRTTEIVFRAVNGLPCSEKDTGELE
                     THLSEVSA"
     gene            complement(7224..7730)
                     /locus_tag="VBC81_001291"
     CDS             complement(7224..7730)
                     /locus_tag="VBC81_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912335.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQDNMIKKKRNPRVLFRNVAYKL
                     SEIEGKTLTEIASFLGFGNSEACRITLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKANIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            complement(7816..7992)
                     /locus_tag="VBC81_001292"
     CDS             complement(7816..7992)
                     /locus_tag="VBC81_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEO6912336.1"
                     /translation="MTATVRRGRPTESKKDYMLRVRLDDETKSKLEQIEKKTSLSKSE
                     IIRNGIDEQFDRLK"
     gene            complement(8217..8390)
                     /locus_tag="VBC81_001293"
     CDS             complement(8217..8390)
                     /locus_tag="VBC81_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO6912337.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            complement(8489..9127)
                     /locus_tag="VBC81_001294"
     CDS             complement(8489..9127)
                     /locus_tag="VBC81_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO6912338.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSAEELSKYRELEHKVIVLLELNAEYNDIKKLVL"
     gene            complement(9351..9593)
                     /locus_tag="VBC81_001295"
     CDS             complement(9351..9593)
                     /locus_tag="VBC81_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEO6912339.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPNADREQFEAITQE
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGNY"
     gene            complement(9620..10240)
                     /locus_tag="VBC81_001296"
     CDS             complement(9620..10240)
                     /locus_tag="VBC81_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO6912340.1"
                     /translation="MRTETWNGYTIRFVEHQGEWWAVLADIAKALDLNPKFIKQRLGD
                     EVVSNNHVADSLGRQQEMLIVNEFGIYETIFSSRKKEAKTFKLWVFEIIKQLRQSTGL
                     EGFQVFRMFDKEHQKKAMNRLVDGLQNATKKDLIKANTIANKAVSDLYGYPKMVSKNE
                     MTENMLRDREPILDETVELIKVKEKYGLNFSVSEAIYNQNTIKKAQ"
     gene            complement(10264..10464)
                     /locus_tag="VBC81_001297"
     CDS             complement(10264..10464)
                     /locus_tag="VBC81_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6912341.1"
                     /translation="MDKLRGYRVMLGLTQKDMSDKLNISLQSYNNKETGKSAFNDKER
                     LAIKSMVSEIKPDITIDELFYS"
     gene            10670..>11057
                     /locus_tag="VBC81_001298"
     CDS             10670..>11057
                     /locus_tag="VBC81_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912342.1"
                     /translation="MNRLKELRKEKKLTQEELAGEIGVSKITILRWENGERQIKPDKA
                     KELAKYFNVSVGYLLGYAPNKKIDFQLNLDGTTLHLTKEQFLALENTSKSIKKIKNTI
                     NESVKQEEYIKNASKYYDFEKVSRRLT"
BASE COUNT         2991 a         2142 c         1910 g         4014 t
ORIGIN      
        1 tttaaatatt ctatccgctt gtttaatttc ttttatccct tcaaagtcag tttcgataaa
       61 ctacagtgac aaaatattga aaaccaccag caagtccatt tgtttgtgtg ttaacagaga
      121 taacttcttt ttgattttcc atgaattgat tgatttcttt atcaaacttt tctatcaatt
      181 cattaatggg tgttggttta aacgacttat ctggatatgc cacatataag atataaaggt
      241 actatgattt attaatttat tttatttaga attccatccc tataatgctc cgcaaactca
      301 agcaaagctc ttttcttagt ctcgtagtac caactttcag agcgattaag tttcgtcatg
      361 atctcttgat tagataactt ttcactaatc aagtaacact cgatcagtat ttgtctgtag
      421 tctatcttag acagttggtt aatggcatac ttaattgcac ctagttcctc tagggcgcat
      481 tctcggctta tttcaaggta cgttctgcgc gtggaatgat attctatatc aaactggtaa
      541 agctcgttat aggtcaaatc aaggctattg gcgatacgtt gccacctctg gaactcctta
      601 agtttacgca tggcgttctt cttgctcatc taacacctct aaagcttctc tatgtagttt
      661 gaaaacagta ttccttgaac aacctacttt atctggtatc tcgtcccatg ataagtcatc
      721 cacatatcta gccttaataa cagctatctg attctcatct tgtagggagg taatcatggc
      781 tagcctcttg tcacgttctt ttgccaaaca taagagttgc tgtgcagtac ctttttcaat
      841 gccatttagt aactcaggat tacgaaaagc attcatcagc ttgatgtcct tgtctcgttg
      901 ttcctcaaat aaggtcatta aagcaaaaag tggcttcaat tcttttaatt gttctttagc
      961 gcccatgatt aactcctttt tggtataatt tagttaagct taaatttaac caaggagacg
     1021 ttctgtgtga gcgtcttttt gtgtttctcg ttttgtaaac tagatatttt ttgtagtact
     1081 tcgcttttgg cttctaaaat cgtttctaag cgctttttaa agttggtaat ataaaatatc
     1141 aacttgtcag atagaagttc taaaaccatg ttttatttta gtctgataaa gtcattcttt
     1201 gtggtagggt acttagctta aattagatat aaaatccttt agaatcaagc ttttaatttt
     1261 tatcaaaaat ttcaaaaagg gaattttttg cacggaaaag ggcggcgttc ttaagtttcc
     1321 gaacaatgta gccccgttca aaaacaagtg gggtatttcc cgaatgttat agccagtgcc
     1381 tgtctccctt atccatatag taatggaatc tcttccaatg atacaagtat ttagctattc
     1441 tcatgtattt tggacgctta ggaaagtcat catgactata ataaccatgt ttgtgtttgg
     1501 cttttggatc tactttcaaa cactctttaa aggctaatcg ccagtagtag caacagtctg
     1561 tcttgcttcg attgagtgtc gcttgatgaa ctttctgaca agaaccacaa gcaaaatcat
     1621 gagaagcctt aaataacttc cggcaacgtc tctcacattt aggacacaag aagaagtagc
     1681 gtttaccacc ataagttcct ggtatcgttt caagtaataa gttttgatgg ttataatgaa
     1741 taaccagatt atctaggtct atgcgaatag gttggttatc cattgttcct gtaatctttg
     1801 tcttcctctg tgccttcatt ggtttaatga tactttcaat agctagttct aacattcttt
     1861 ctccttaaaa ctcaaacaac ccaaaactat tgataaaacg acaaaaagag ggaaatccct
     1921 ctgattgttt atttaacgag taactgacct tcaacaacca tatcatacaa atggttaaag
     1981 gcttgactga tagtttcaag gatagctcct aaatcgtctt ggctcatctc ctgataattc
     2041 atagagagat gttcagctag ttggtcatgg tctgagatga aagccatgag tgtgtcttgt
     2101 tcattagctt cctcttgagt aatctcaaat aaaggaacca cgcgctgatc atcaagttct
     2161 tcaacttcct taattaattc attttcctga gccatgtcat caagttcttc atccgtcata
     2221 tcctcagatt ggttgtaata gtccttgaaa ctgtcacaga tacgcttgaa gaccttgctt
     2281 agtttcttat cttccacata ctccagcact aactgaccct tgcctttaat cgttacccca
     2341 atcacgggcg cgtgatatgt cccagtcata taccctagaa tggcatggca agctacctga
     2401 gcggtgtcaa agtctttaaa agtgtagtgg aatgtgaatg tttttggtgt gtctgaaaat
     2461 gttctcatgt tatttctcct ttgtgattgc tataatgtct gataaattga taatggcaga
     2521 aggacacgct acccagtttg gttgttggcc agataaaaga tatttgacca actcattata
     2581 aagggtgcgg tctccttgta tggtgatggt gttaccacct cgtgtgtgta attgtagttt
     2641 catatcagtt acctgtacaa gaccaataaa gaagttgtgg tactcatgtc ttcatagttg
     2701 ccataagttg cttcttgata tttaatatca atcactttct tatcagcaat aaaagaattc
     2761 accctgtttt caaattcctc attagtttca tttctgaatt ttggcaaatc catatactcg
     2821 ttaaagcctg tttcaattcg cttgcgatta aatagtttaa ttttcatttt tctttatcct
     2881 ttctgatttt gtgacatttg tgacattgtg tgacgttttt aaaaaactcg taatcccttg
     2941 gggggcaatg gtttgttaca ttttttactt ttatgacata ggggttaaac tctataacta
     3001 gatataagag taaagcactc ttctttctct tttatagcaa ttatatctaa tgtaataaat
     3061 gtataaaatg taacaaagcc ttggggctgt aagggtagct cgtgtgacgt tttctaattg
     3121 taatgtcaca aatgtcacaa actacttgtg ttttgctttg ctattccaat accctctatc
     3181 ttgttttggt ctcttgcgtt tctctggtgt ctctctaccg tttgaatagg tcgcaagact
     3241 agcataatcg ggtgtatcct ctttagggta aaaacctaaa tgtaattgcc tacctgatgg
     3301 gatagttctt acaccagttc taatgtaagg tggtaaaacc tgcttaatat ctctgtgcaa
     3361 tcctctttcg gtcttaaaaa acgattgatt aaaataatct tgatactgtt tccagcaata
     3421 ccaaataaaa tcattgggaa taaattcact cgctaaatct tctctaaacg tcttttcgac
     3481 aaaatccaaa acagggttaa tgtccttttg gtgttcacct aataaggttt gagattgctt
     3541 aggattgata tcctctgatg gtgtttcaat tgctaactta accaaatact ctaggacttc
     3601 tttgcgattg atataatctt tcttgatagc tgggtttggt ttatctttaa atctgcttga
     3661 aaatatcaag attctaaacc gcctgtcaat tgctgataca tcaccattca tacgtgggag
     3721 accattggaa gattgtacaa cggtcatatt taacctcaac gaataaggtt ttttcccttt
     3781 gtcctcaatt gtcataatat caccagtcgc aaggctgaac atgtccgagg tatctttgat
     3841 aatagcgtct ttttgaacgt catcaccgat aactaacgat ttcccaagca agatagaagt
     3901 ggtaaaacgg cttttattta agtctgtaat tttaagactt gcaacgtttt ctaaaccaac
     3961 taaatttatc agtaactgct gaaaagttcc tttacctgtt ccgccctctc catataacca
     4021 aaaaattttc tcgagcgatt gacctgtgat actcgctttt ataatttgaa tggcaagctg
     4081 gtataactcc ttatcaccgt caaacagttc ttttaaccat tctgtcactt tccacccatt
     4141 tatagtaggc tcttttgcat aaggattgta ggcggttttg atttttctag tgacaatgat
     4201 tttaggattg aaaggttcaa actcgcctgt tttgttgtta aaaagttgtt tgccaataac
     4261 cgtatacttt ccttgaattt ctttcatttg gctttgtctg gcaattttat aaagggtgtc
     4321 aaaagcctgt ttttccgttg cgtttgggaa aattgtagaa ataaggtctt gtaaaagttc
     4381 gttatcctct aaccagatac caaaatcagg gttatagtag tacagagggg ctttctgtcc
     4441 ttgcgcttct ggtttaatcc tgataaagtg gatatattgt ttcagcatga tagcaacccc
     4501 tagcggtgtt ttaggcaacg ctttttcgct tgcttcttgt cctttttggt gggctagttc
     4561 tttatgctgt acctcggtca atctgcccgc ttttacattc tcaagatgtt cgctgtctgc
     4621 catgacctca tcataagcta tctgataggc ttcttcttta atactctgac attccttaat
     4681 aagttgcccc ctaacgcttt tgaaagtctt gaaatactta tcttcacttt cacgcgcctc
     4741 taagatttca ctttcaaggc ttttcaaatc ttctttttct atggctctat cctctctttc
     4801 taaattctgc tcttgctata ctggtaaaag tgcggtctaa ctcctcaatc ggtaatgggt
     4861 tatctgtcac accgttagct atctttgtca actcgtaagc tgtttctata tcacaatcaa
     4921 cccatttatt aaatagcaaa ccaacaaatt tagttaaggc tacattacgt ccgccttcgt
     4981 ctccaaaacc attaaacaag gtatctatga ccctcatggt aatagaacgc tgacttctag
     5041 ggcgtgacgt gtaagtagta gtaacctgtc ggttgggtgc tttagggaca ggataatcaa
     5101 gaccacggtt cacatagcgc tgatagtctt ctgggtcgcc tgttgtgacg ggtaagcctt
     5161 gtaattgcga ccaggtaaga ctagctaaat caaacggtag tccaatctta ttagctatct
     5221 ccttgaccac ttgtttatac gtcgttttgt ccatagtgtc actaggcttc acgacaaggc
     5281 gatagcgtgg cttctcaggg gtgtgtttaa tcgttggata aataatatag ctgtattccc
     5341 aaagcgtctt agaaacgatt ttaggtaggt tagcgcctgt ttctatctcg tcatagtcaa
     5401 gaaaaatcaa atcgcgataa actaaactag cattattgcg cttatagcca ccgtttttct
     5461 ctgctgtgac tttgccactc aggcagtaag gggcttgcgt tcgcttgtat tcttcaatat
     5521 tgattccttc aggaggtttc aaaggtttaa actgagcaat atagtcaaat ggttctaatt
     5581 gtcctttata ggggtacaaa taagagctaa agcctcttgc ttcataaata gccatctaca
     5641 catttacccc caaaaaaata agaatatcac tgactttata gtaatgcttc ctcgtatctt
     5701 ctagtggtgg ttggtatcgt cttaacccag cattttccca ccgttttaga gttttgcctt
     5761 tgatatttaa ttcctctttg acctgttcag ccgttatcaa ccctaaaact cttggtttag
     5821 gtttctggta ggcttccaaa aagcgattaa aagcggtcag gttttgttct aagagtttgg
     5881 cttcataatc ttgactaaat aagctcatgc ctaacctcct ttgagtaatt ccttataact
     5941 ggttaaatcg gcattcaata acacacttag gcgttcctgt tccttttgta cttgattata
     6001 aaaggcttta gcaccatcta gtaattcttc tttgttagct gggataaagt acccacgatt
     6061 gaatccgtgt ctaatgccga taatagggat gttatagcgt gtgattaagc tactgatgat
     6121 actttggacg gaacgctctt caagtttcag tattaagcta atctctgccc ctgtaatggg
     6181 gttgtctgct ccaaccttga tcagattaag gacacgtcta taattctctg gtagtgtcat
     6241 ttcgtgtcct ccttatcatt tggaaacagg atttggttat caataacacc aagcacttga
     6301 tttttaagac tatcttttaa ctcgttcaat gcttgtataa ctatatttag ccctttttta
     6361 tattccatct ctattggtat tgattccaaa aatttaacaa cggcatcagc tttgttatag
     6421 tcacgttgtg tagtattgat aaatattttt ttcgtgaata acggaaaatc gtcccactct
     6481 gcttcaatat cttcatctag cattttgata tttttgtcta aaaccctatc actcaaccag
     6541 tttgtaggct gtttttcgct atcttctagc aattttttaa aatattcttc cgttgtcatg
     6601 cagttccctc cctaattgta ataatggttc tgtgattgaa tataagcccc gtagtttgcg
     6661 ttctgacgtg gtttagggac ttgggtatct tctggtaaat caatgtctat taatgactta
     6721 gaaaggctaa ggagaagccc taagagacct aaaacaatga ataaaataag tgtctgtgtt
     6781 ggtgtgaggt taagttcttg catcatgccg atacctcact taaatgagtt tctaattccc
     6841 ctgtgtcttt ctctgaacaa ggtaaaccgt taacggctct aaagacaatc tctgtagttc
     6901 gttgatagtc taaagcttcc catgcttctt caaagctggt ggcacttttc ctgaatgtag
     6961 tcgtatactc tgccattaca ttagcaataa tcacccgagc aatatgttgg ttatatagtc
     7021 gagtgaaata tgcttcagct ttatctttgc tgagttggcg atttttgaac atttctagct
     7081 gttcaggagt gtatctatct tttgaaaaag gatttgtttc tactctatat ttcattatgt
     7141 cttttctcgc ttaattttat tttctgtgta attgccggtt tcctatacta gattcatgct
     7201 agttttaagg ggtagctccc tgattagttc atgttagtgt ataattctgc aaataactcg
     7261 ctaggaatac gctctagcgc tttttgttgt aagtggatgg ctttaattct atcttgtgtt
     7321 ttggttttaa tgtcttctat aatttcggat gttgagacca cttgttcata gtaaatgtta
     7381 gctttataaa tgagtccttg ctcttttaac tccttgttag ccattttttc aagcgtaact
     7441 tcatgtaata cttcaacatt acgataatga ccgtttttga ggtcgaattt tagccatttt
     7501 ttacgcttcc atttatatag agtgattctg caagcttctg aattcccaaa accaagaaaa
     7561 gaagcgattt ctgttaatgt tttcccttca atttcagata gtttataagc gacgtttcta
     7621 aataacactc tcggatttct ttttttctta atcatattgt cttgattttt gagcacacaa
     7681 aaagcgcact ccctttctgt tttttgtgtt gactgaaaaa gagtacgcat gtataatatt
     7741 tacgtacctg ttttcaggtc ggaagctcct agcgtgtgcg gtcgccaaac tacaacacgt
     7801 taggggcttg tttttctatt tgagacggtc aaactgttca tctattccgt ttctgattat
     7861 ctcagacttt gaaagagatg ttttcttttc gatttgctct agtttagact tggtttcatc
     7921 atcaagccta accctcagca tataatcttt tttgctttca gttggtcgtc ctcttcttac
     7981 agtagctgtc atagagtttt atcactcctt tctgtcgcta caaaataaat tatattatgt
     8041 agctacataa tttgtcaacc cctaaattaa aaaaacatgc gtattctatt ttcaatgtgc
     8101 aaatggttca caaaatagag tacgcatgat atactattta cgtactcact ttgtgggtcg
     8161 agcgatagca tagtaaccaa aaattggcgt tggcggttac tgtgctattt ttttgtttag
     8221 tctgacaaat cttcaagggc ttttctgatt gcttctggct ttgttaagcc atgattatca
     8281 gcatatgctt gtaccttttc atcaagtcct tggctaattc taaccgttat cttgatattg
     8341 ttcggttgct cgccctttgg tggtctgccg actgctcttt tgtcagtcat tgtatacctc
     8401 cttagttttg acgacataat tattataatt aagccgtctt aataagtcaa cccctaaatc
     8461 aaactttttt gcgtactcta ttcagttgtt aaaggactaa ttttttaata tcgttatatt
     8521 ctgcatttag ctctaacagg acaatcacct tgtgctcaag ttctctatat tttgataact
     8581 cctccgcact caaacaatct aatgcaattt cataacctac ccgtgactgt ttcagttgtt
     8641 tagcagtctg tcctgtgaca gattttagca aaaggtcgct aatagccttg tagctccatt
     8701 gattaaagtg ttcccaatcc ttgatagctt gtgttaattc tttatgatta gacttttcaa
     8761 gctctcgtat cagtttaaag ttagcgtttt ctctttctag ttcttcgata tggtcataaa
     8821 tccattgacg aaagatttta cctttctccg ttttggacag cataccaatt tcaaaaatac
     8881 ctcttttatt gaatagtcgg gtctcgtatt gtttgccatc agtagctgac aatttgtcag
     8941 taactgaaaa tcgcttttct ttgaggtagg gttggcgctc tatcatcttt tcaatagcat
     9001 ttcggctctt atatccaaat ccctgcgcta attgttcaat cgtcacaaaa atatttctat
     9061 tcctgtccag ataaaaatca atttcaagtt ctccaaaaga actttttact tgttttatga
     9121 tattcactgt tttacctcgt tatttgatta ttgttgtttt gtcggggaga actgcaactg
     9181 ttaagtcgtt gcctgctctg cggtagtatg atacaatgga agtatcaaat cttttactaa
     9241 aaccccttta ataatagctt gcctgcttta ttatttgagt ttagttatac tagttaaagg
     9301 ctgtgctgat tggtctcggt aagccttttt ttgttgctct tgattattga ttaataattg
     9361 ccttgttcaa tgtcattcaa acgcttttgc tctgctttgc gattatagat tagcactttg
     9421 tcatcaagca tgagcgatac gccttccaat acgttgaaaa tttcctgtgt gattgcttca
     9481 aactgttcgc gatctgcatt tggtactttg tcagcgtaac cctgtgctag ctcagctaaa
     9541 tcaacacctt catcaatcca tttcttcaac tctttgtaag ttgttgcttt cataatcatt
     9601 tctcctttat ccacgcgcat cactgcgctt tttttattgt gttttggtta tagatagctt
     9661 cagatacgct aaaatttaag ccgtattttt ctttaacctt gattagttca accgtttcat
     9721 caaggatagg ctcacggtct cgcaacatgt tttctgtcat ctcgttttta ctaaccatct
     9781 taggataacc atataggtca gaaacggctt tgtttgcgat agtgtttgct ttgataaggt
     9841 ctttcttagt tgcattttgt aagccatcga caagcctatt catagctttc ttttgatgtt
     9901 ctttatcaaa cattctaaat acttggaagc cctctagccc tgtgctttgg cgtagttgtt
     9961 tgataatctc aaatacccat aatttaaagg ttttggcttc cttcttacgg cttgagaaga
    10021 tagtttcata aatgccaaac tcattaacga ttaacatttc ttgttgacgc cctaaactgt
    10081 ctgcgacgtg gttgtttgaa acaacctcat ctcccaaacg ttgtttaata aattttggat
    10141 tcagatctag tgctttagca atatcagcta gcaccgccca ccattcacct tggtgctcta
    10201 caaatcggat agtatatccg ttccatgttt ctgttctcaa taagttgtcc tttctttaat
    10261 cttctagcta taaaataatt catcaatggt tatatctggt ttgatttctg aaaccattga
    10321 cttaattgct agtcgttctt tgtcattaaa agcgctctta cccgtctctt tattgttgta
    10381 agactgtaaa gaaatattca gcttgtccga catgtctttc tgggttaacc ctagcatgac
    10441 acgatagcct cgtagtttgt ccatgatgta tcctcctttt gaaaaaattc ctcccgtagg
    10501 ttgaaagtga gaaaggttgc gggagataaa agtatccttt tcggataact tcaaaacgat
    10561 tatatatctg tttcggttac ttgtcaagaa ttattttgaa atttttatat catttcttga
    10621 tactttttat aagttcggtt ataatcaact ttgaaaggta gtgaaataaa tgaataggtt
    10681 gaaagaatta cgcaaagaaa aaaagctaac ccaagaagaa ttagctgggg agattggtgt
    10741 atcaaaaatc actattctcc gttgggaaaa cggcgaaaga caaataaagc ctgacaaagc
    10801 aaaagaatta gcaaaatatt ttaatgtatc agttggatat ttactaggtt atgctcctaa
    10861 taaaaagatt gattttcagc taaatttaga tggtacaacc ctccatctaa ccaaagaaca
    10921 gtttttggct ttagaaaaca cctcgaaaag cataaaaaaa ataaaaaata ccatcaatga
    10981 atctgttaaa caagaagaat atataaaaaa tgcttcaaaa tattatgatt ttgaaaaagt
    11041 cagcagaaga cttacgg
//
LOCUS       DATRNX010000060        10660 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRNX010000060 DATRNX010000000
VERSION     DATRNX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..140)
                     /locus_tag="VBC81_001299"
     CDS             complement(<1..140)
                     /locus_tag="VBC81_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6912343.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCT"
     gene            complement(233..874)
                     /locus_tag="VBC81_001300"
     CDS             complement(233..874)
                     /locus_tag="VBC81_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6912344.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(855..1439)
                     /locus_tag="VBC81_001301"
     CDS             complement(855..1439)
                     /locus_tag="VBC81_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6912345.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(1509..1654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1723..2322)
                     /locus_tag="VBC81_001302"
     CDS             complement(1723..2322)
                     /locus_tag="VBC81_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO6912346.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKIGNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(2315..2986)
                     /locus_tag="VBC81_001303"
     CDS             complement(2315..2986)
                     /locus_tag="VBC81_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912347.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(2976..3851)
                     /locus_tag="VBC81_001304"
     CDS             complement(2976..3851)
                     /locus_tag="VBC81_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6912348.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(3889..5397)
                     /locus_tag="VBC81_001305"
     CDS             complement(3889..5397)
                     /locus_tag="VBC81_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6912349.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(5438..9742)
                     /locus_tag="VBC81_001306"
     CDS             complement(5438..9742)
                     /locus_tag="VBC81_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO6912350.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDISVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(9762..10331)
                     /gene="lepB"
                     /locus_tag="VBC81_001307"
     CDS             complement(9762..10331)
                     /gene="lepB"
                     /locus_tag="VBC81_001307"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6912351.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(10315..10587)
                     /locus_tag="VBC81_001308"
     CDS             complement(10315..10587)
                     /locus_tag="VBC81_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912352.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
BASE COUNT         3268 a         2043 c         1710 g         3639 t
ORIGIN      
        1 gtgcaaaaag tgcaccctcc tctagcaact gttccatctc tgttaggaca gtcaaaacca
       61 gcatctatag gtaatttaaa tattttttct ccaaagagtt ctcgataata atcattaatc
      121 gcacgataac gttttttcat aggataattg tatcacaatt ttaactaaaa taacctcact
      181 actacaataa aactaaaaaa gattggaacg tcagttagtc ccaatctttt atttacttca
      241 ctttctttaa ccaatccttg gataaaaaga tatacgcagt tagattcaaa ataccataag
      301 caagtataaa accagctaaa acatctgtcg gaaaatgaac ccctaggtaa atacgagata
      361 acccaattaa aaaaatgagc aaacccaatg taccttggca caacagtttc catatactct
      421 taggcatata gtactgcaat aaaataatac tactcccaaa tatcataaat gttcccatcg
      481 agtgcccact gggaaacgaa tagccacctg caaatactaa atgggttaaa gttggtctca
      541 ctctttgaaa aataagtttt aaagaaagta tacatatacc agagataata gcatttactg
      601 cgataaatct agcttgagga taccacttct taaggtaaca gaaagtgacg ctcataatcg
      661 caatagctat ctggcttaca gtattaccaa ccacagtgat taacttaaaa aatcttgtag
      721 aaagatttgg caactgtcct ctaacacttt cttgaatagt ttggtcaaat gcgattacag
      781 tgtcgggcca atatttgatg accaatccta aactgaaaaa taagataata gcaataaatg
      841 cttgaataag tttactattt tgacgagata acattagtct ttttatatct ttctaatatt
      901 ggcaaacaag ccacgtaagt tagatagaaa acaatcgaaa ttaaaattcc ctcaacgata
      961 ttaaatggaa taaccattgt taaaaggtaa ttgcctacac caataaatgt tctgatatca
     1021 aagttagcaa atatagcata caaaggaatc gcaaagacat agttgagagc taccatagat
     1081 acagtcaagc taactgtacc aaataaacta gctttaataa aatcttttgc actctctcta
     1141 tttttccaga aaatagcgaa acttgctaaa aatagagcta gagcaaccat attcatcggt
     1201 aaaccgataa aggtttctgg accacgatta gcaagtataa cttttaaaag tgatcttaat
     1261 aagagtacac cataacttga tttcaaatca aataaaataa aagcaactaa catcggaagg
     1321 attgaaaaat caacctttaa aaattctgct cctggtatta atggaaatga aaccatcatc
     1381 aatacaaaag ataaggcaga aagaatggcg attgtcacca ttttacgtgt atttgtcata
     1441 aaaaaattcc tccaatttaa ataaattgaa agaagctcca aaggtaagcg taggtacgcg
     1501 aaaaaaacct ttgtcttctc ccatccagac tttactgtcg gttgtggaat ctcaccacat
     1561 cagctttcgc tcgcggactg atgcttcaca actgacaaat aagttggaag cgattaccgc
     1621 cggtcgggaa ttacaccctg ccctgaagac acctatagca taacaaaaaa aacttgcaat
     1681 tgcaagtttt ttaatcacta attagtagta gattgtataa tattaatttt taacatcaat
     1741 taattgacag cgcactaata ctctagctac tcctgccttt gtacaagtaa acaagcttaa
     1801 gtcccaatca ttgtctgatg tggcagtttt ataaactttt tcaatcgctg ttggttcaat
     1861 aatttctcta ttaccgattt tgtagtgata gatttgccct gttgtagttg taaaataaac
     1921 atccgttccc atatctacat tttttaaagc atcaaaatga taaggaaaat tatgcgcaca
     1981 aatcaccatg ttattagtta aataagaacc ataatacctt gtaggcgtct tagacagttg
     2041 ttcaaaacta taattagcag ctaccggtaa atgcagtttt aagttcggaa tatccagagt
     2101 tcccaagtaa tctgttttat ccaacttttt tacaggtaat tctccatttt ctgaaccctt
     2161 tacttgatgc gtaatagatt tatcaagcac cttgacaata tgctgagaag ttaaatcagc
     2221 ttgatgcgcc tgattaatat tataccaacc ccaatagatt ccagaactta ccagaataat
     2281 tccgagtata gctaaaaaat ttgctgaata tcttctaatc acgtcttctt ctccatttta
     2341 aagctattat taaacacaga agtcctgaca taattagtat aggtattggc caccatactt
     2401 gtccagtaaa cggaagcttt ccctttgtct gatgtgttac tgtaggagta attgtctctt
     2461 cttttttagg tttagcattt aaagggctca ttttctcaaa tgctgtaata tcgtacttcc
     2521 catccttagg tattgatagt ataaagggag acattagttc ataaccttga gccgttttag
     2581 tctgaataaa tagataaatc ccttgaggaa gattgttcgc aacaatacct tcagccggta
     2641 aattatcaaa cgtttgtaaa ggttgagttt tatgaacagc ttttgttagt agattgacgt
     2701 atttggcttg gttactatca aggtttactt gtgttagatc atcgtctttt attccaatac
     2761 cttgaaatgg ggtagttaga gtaaaaactt gattaccatc aacatcttta gcttgtgcta
     2821 cttggtaaac aagtaaatta ccgccagcga taccttgatt attatactta ttttgtatag
     2881 taatagaacc cgttttcatc tgatcattgg tatcagcaga cacaagttga gtacttagac
     2941 taaataataa gagaagagtt atctttagga tctttttata aatcattgtt ctcttccttt
     3001 ctcattgctt gttttaaaat tttcttacgt tgacgtgctc tcctagttac ttctaaagag
     3061 attaaaagta aaatcaaagt aaggaaaata gcgataaatg gtgcgatata aataggctct
     3121 atttgtattg cctctgctac taccaaagcg ttaccattat cgtttggtac acgatgtcct
     3181 ctcactagta accgatgggt attaacgcca tatggtgtac acgtcaccaa agtttggtag
     3241 tctttacctt taacaatttg taaatccctc aaatcatccg gtttaactgt tctgatttga
     3301 tccacttgat aagtatatgt ttcatttaag atactgactg tccagtggtc tccaactttt
     3361 aacttatcca aatcagaaaa aagccttgaa gagggtaaac ctctatgtcc tgataaaata
     3421 gaatgagttg agtctcctcc aattggaaga ctacttcctt ctaaatgacc aatagaagtt
     3481 tgaagcactt tttcacttgt accatgataa agtggtaatt ttatgtttat ctttggaatt
     3541 gaaatataac ccatattacc cgttttatcg atagccagtt gtgaattata atccaaacgc
     3601 tcttggctag tcatgtgcca cttcattcct gaagttttaa attgcttatt atattctttg
     3661 gctcggttaa taattttttt ataatcgttt tcatccatat gcgttacgcg gtcttggtaa
     3721 tccataatcg ctcgagattg gtgaaatgaa ttccaataat tagcaagtga aggataagcc
     3781 attaagccta cccccactgc aattatagtg acaagcaaaa tggatactaa atgttgtctt
     3841 atttttttca tatattttaa atctgtacca ctttgctagc ccatcttatt aagaacgtaa
     3901 acgacgacga gcaacaagca cgatacctgc tcctattact aaaattgcac ctataatgta
     3961 gaaaattgtt gtaccaatac cacctgttga aggcaactca gtacctttgt tattttcaac
     4021 agttgggtta actaaaaggt tatctgaatt agtcgtatca gtggctccat ctcctaaaat
     4081 aaccttctga gagttatcta acaaattgta acctaaggga gcctttttct caactagata
     4141 gtatgtacct tctttcaagc ctgtaatggt aattatacca tctgctcctg ttgtatattc
     4201 tgttgcatta gcttctgtgc cccattcaac gttatttgta tcgttaaagt ttagaaattg
     4261 acccgtagca ttctttaaaa caaatatagc accttgtaat gaagcttttg tggaaccatc
     4321 aatttttttt atagtaattt gaccatccct cactgttact ttttgacctg ggtcatcatt
     4381 gctagtattg gggttgatgg tcgcaatgtt tgtattttct ggtaaatcag ctgaacctgg
     4441 tttagctcca ctctttaata ctcctgtata agtgactgtg attgtattta ttcccttata
     4501 aaaaaagtca tcattagctc cattttgagt atttccggtt ggagtattgg tagctgccca
     4561 cggaatagta atcgtgaaat tattattttc ctctaacagg ttatacttcc cagttgcttt
     4621 ttccgaacct tgagttagag ttgtaatatt ccctgatcca tcagtaatag ttacttcata
     4681 agacccttcg ttcaaatcaa ctacagaagc agatggcata gtatccttta taacatattg
     4741 atacactttt tctgtaccat gataattgac tgcattctta taagtaatag tatatttgac
     4801 tgtatcacca accgagtacg ttttttgatc tacagttttt ccaccaccat ctccccatgt
     4861 cgcatcagta ttcttttcat gaatagtagc atttggagtt acagatgtaa ccataattac
     4921 agctccatta ttaacagtgc tagaaacata ataatatcca tattgggaaa cattaataac
     4981 ctcagtacca tcattatttg actcagtaac agtggaaact ggtgtagtat tagctgatat
     5041 agatttagcc catgtcgcaa tctcatttgc tgacgcagta tcttttttag ttacatatgt
     5101 tctccctcca ttagtagttg tcgtaaaaag agaattaaaa tcagttgaag ctttatactc
     5161 agcttcttta ccttgaggaa ttaaataaga agctccatct ttattcgaat cagatacatt
     5221 tgcattatct atttctgcat caaaaacttt atatgcttta taggttgcgc ctttttgagt
     5281 atcttgaact gtaattgtcc ctgtctcagc ggcaaaagct atcggcgtaa ctggtgatac
     5341 agccatacca aatgctaaac tcgccactaa cagcgattga atcattttct ttttcattga
     5401 aatctttctc ctaaaatcat attgatgaat gattaattca tatttttttt cgatagtata
     5461 atattaatcc tgatggtaga gctaaagcta aaccaactag gatataaatg tgtgttccaa
     5521 tacctccagt actaggcaat tctgttcctt tactgttagt aattttaaaa gtatatactg
     5581 tacttccatc cactaaattc tcttttattg gtgtcgcatt attaccattt tgttcaaagg
     5641 taactcccgt agaaatcact aatactgata tatcattttt aggtagtagg taccctggag
     5701 gggcctttgt ctctgttagg tagtattttc ctactggcaa actgaggtag ttattagcat
     5761 ccactaataa caagccttta tcgtttgtca ccagccctga atacatagga tgtgaagctt
     5821 tattcccatt agcatctgat tcataaatat caaaaactgc acctgctaaa aaattattat
     5881 cattttcgac attaactttc tgtagtcgta ctttttgctt gatacgtgta ttggtaaagc
     5941 taatatctac gtctcctgaa actgtcaggg attgtaagcc ggtagcatca taagttttat
     6001 cagcttcacc agttgctaga tttttttctg taattgactc agatacttta aattcatcgt
     6061 aggcttgttc atctattgat atagaagttc cataaggtac tttaaattcc ttagtctgac
     6121 catctctcag cggaaaattc tcttgttgca acgtttcact tggattaaac aagaagtctt
     6181 tcgtcttatc ttcatctagt ccaacgacag ttttacttac tctgacggtg tattctttag
     6241 gttgccaaac agcatataag gtatttgttg catcagggtt gttatcaata cctattgatt
     6301 gacctgctgt aaattccaca cgtcctgtat cagctaaatc cttatcatga tgccaaccaa
     6361 taaggttgta acctgtcctt gtaaagtatt ggttttcagg aattgtagtt gtgctattca
     6421 actccatacg cggtgtctcc acttgtgtta ccacattacc attttctact ctagtaccac
     6481 cgttaccatt gtatttgatg gaggtatctt ctaatttgat atctcctact ggaataatga
     6541 caggttttat ggtgatattt ttattagcat ctgctaaatg ggcgtcaata tcaatggaat
     6601 catatgggtt ataaatttta ccattgtacc accagccacg gaaacgatag ccatctggca
     6661 ttgtcggacg tctcagtaag gctgaatggg agccatcgtc ataggaatca gggtcagtag
     6721 gctcattact tgtttgtaat tgctgaccag aagcatccaa tgctggcttt ccatctgtac
     6781 caacagcatc attgctgtat ataatatgat aatctccagc ctttcgccaa atagctctta
     6841 aattgatatc ttgagttaca gcacctgaaa agttataagg tctaatacta ccatctggat
     6901 taacataata ccaaccgact aatttgtaag cgtcttttac gtacttgtac ttagttgttg
     6961 tatcaacatt tgagagacta gtatctgtcg tataataggc atctttagtt gagtcgggat
     7021 ctttatctcg tgaatcatac ttataataat atgtacctga aggatcttgg atataatccc
     7081 ttgtaatatc tgtataatcc ggaatacgat caccataatg caaatctaaa taggtatcat
     7141 ctgtttttga taatcggcct ccgtttggat caatattgac acgatatgtt accttttgcc
     7201 aacctgcata gactttaaca tcatgaggag gcatagtcgt gttaaagtca aatacttgtg
     7261 tttgtgcttg gtctttatac catttaccat cccaaacata ccctggtcga ctaggtttag
     7321 gttgaacagt tgtcgtatcg ggggcataag aggacaaatt ttgctcatat agaacatcct
     7381 ttactggaaa attaggaagc tctgtattat caagcggatc taaatattta atcttgtatg
     7441 aattacgttc ataccatacc actaagttca aataatcttt gtggtaggca ccatatctat
     7501 catagtattc atctgaaata ggaactttta ctcctgcact cgtttgactt gcgtactgat
     7561 caaaaaggta attatcagga tataaacgtt gataatattt aacattaaac cctagatatt
     7621 tttctgtaaa ggtaaattca tctgccccag cacctccacc ggtatctgct aaagaataag
     7681 tgccatccaa accttgtttg aagaaccgat aagtttgaac tatattccct tttggaaaga
     7741 gtttgataac atctgaatca acggtatcat taggtaaaat gaactcaccc aaataactca
     7801 tcccataggt tccaacttgg ttatttccaa cattggctaa ataaccccat gcacccgtct
     7861 tccattgata accattagcg gccaaggttg taccgtaaag tcctgtgtaa gtatcagcat
     7921 cagagctcca attatacgaa tagtaatatt ctggcaagga atatccgcca tattttgcaa
     7981 attttattgt gatgagtttt ctatcataat aaacattaac aacacttgaa ccatcgtctt
     8041 ttatcatgac agaagtttct gtcctcgtat tattaacttt aaagccagtc ggtagtttat
     8101 cattaatatc ttgttgtgtt agcgtctgat tagataaaga taggcctgat cgtgttactt
     8161 gccctgcgta ctcatatgtc ttttgcgcat cagtagcatt tttattatcc gttgctgatt
     8221 gttgccagta gtttatcgtg taggttgttt gtgcgggcga ccaatgtgca tatagcgtcg
     8281 tatccttggt caagacttga ttaaaatcaa aggctgcccc accactagca gctgtgtacc
     8341 accctgcaaa agtataacct ggcctcgttg gatcattagg cttaattgtc gaagcaggtt
     8401 ggcctgttaa cacacgacga ggtgcaatat aggtaactcc tgttgataag tcgcctgtgt
     8461 tgaattcaac accgtacgat tctttaaaga caggataaag acttatgtct gaagatacag
     8521 gtaatgcttg aatttctgat tcagaaagtg gagctccatt ttgagtttta gaccaaccta
     8581 caaataacaa tgttgaagtg ggtggtgaaa ctttaaatga acttaaatcc gcggttccgt
     8641 ttcctgaact atccaatggt acttgataac gctccaaaat acttttacca gcagaatcat
     8701 catataaagt tactgtcgcc actttactca taaatggacg aacacaaatt tcttttgtct
     8761 cagttacagt tattggctca ccaaatttaa cagggtcacc atactttcca gtagtaggat
     8821 cataggtata ccaaccatta aaatgctctc ctgctttaat cgtcggaatc ccaacttctc
     8881 ctagagattc tccatctttt ataatttgat gatgaacttg catacctgaa gctgtcagga
     8941 aatcataatc agttccgtca ttattttgaa aatggtaagt taacctagga acttctgtat
     9001 tatcctcttg aacaattgca taaatggaga atgaatctgt tttaaaagca acatcactgc
     9061 tagtgttctt agtttctgca gtatctttag attttaatac ttctgtttga ccatcatctt
     9121 taaagtgaac aactttaagg ttttcatctg aagcttctaa tggcttatca tagttaacct
     9181 ctacttttac tggagcttgg ggttctgctt ctttaccatt tgactcaata gtaatgtcat
     9241 agagtttgaa atttttgatt tcactatctt gtttagcaac ctctgtcaat gcttttttct
     9301 tataatcttt aaaagtagct gaattgtctt ttaattccgt cacctttaaa tcagcatttt
     9361 taggaatctt agcttctttg gtcaaagtca ctgttacagt atagtctgca cctctaaaca
     9421 tcaatggttc ttcacgataa gcagcttcct cagaagatga tgtttctgtt acactagaag
     9481 caggagtctg gggcttgctc tgctcaacac ttgattgaga actagatgtt gatgaagtta
     9541 cctggctaga atttttattt tctaaagtaa tccccacatc atctgtctta gtttcttcaa
     9601 ctgttattgc tggtagaatt aaaaaataag tcgttaaaaa agttgttagg atcatcaatg
     9661 accacatgat aattttccac tctttagggt gttttttctt ttttaatgat tcgattataa
     9721 aagtttgaca cttctttagc atttttgcat cctccctaac cttaattgat actactaatc
     9781 ttacctagag gccatattct gaaagaaatt ttacctacaa tttgttcttc tgaaacatct
     9841 cctacagaag tacttcgaga atcaattgaa gtttttcggt tgtctcctaa tacaaaaatt
     9901 tttttatcag gtacttgata tgggtatttt atattactat taccgagtgc tttatgaata
     9961 acatatggtt ctttcaactt atgttgattc acgtaaacat ccccttgaga atcaatatta
    10021 acccagtctc ctgactctgc aataacccgc ttgactagga ccttattatt gtagtaaaac
    10081 gcgacaacgt ctccagtttt aaaatttgaa ccttttactg taaagactac atcacctgca
    10141 cttaaagtct tattcattga atgtccgtag attcttaata caggcaacca taaaaccgca
    10201 attaaaatgg ctattgaggc aaccgccatc aagatgtata tggtattttt aatgacactc
    10261 caaaagcgtt tctgataagt tacgcgatcc agttcttgag ataatttatc tgaactaatc
    10321 tgtcttttca ttgtctacgc ctctcttatc tactaaattc tttacgtttt ctaaataggt
    10381 atctgcagct aactgagcag tctcaaaaat accactaagc attaaggaag cttcggcaat
    10441 agaaccagct ttgctaattt tgattttttt atcatctagc gcttctttaa gttgctgaat
    10501 ctctttctct tgtttttcaa taagaagttg ctgttctaac ataatttcta gcaagtcttt
    10561 tctttttaat ttttttaaat cttccatcgc gattacttcc ttaactgaac cttaaattat
    10621 cgtttagata ttatatcaaa gttctaacct ttaaactcat
//
LOCUS       DATRNX010000061        10540 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRNX010000061 DATRNX010000000
VERSION     DATRNX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            73..744
                     /locus_tag="VBC81_001309"
     CDS             73..744
                     /locus_tag="VBC81_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912353.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(815..2815)
                     /locus_tag="VBC81_001310"
     CDS             complement(815..2815)
                     /locus_tag="VBC81_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6912354.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(2950..3711)
                     /locus_tag="VBC81_001311"
                     /pseudo
     CDS             complement(2950..3711)
                     /locus_tag="VBC81_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(3773..4765)
                     /gene="pta"
                     /locus_tag="VBC81_001312"
     CDS             complement(3773..4765)
                     /gene="pta"
                     /locus_tag="VBC81_001312"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6912355.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(4791..5675)
                     /locus_tag="VBC81_001313"
     CDS             complement(4791..5675)
                     /locus_tag="VBC81_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6912356.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(5672..6508)
                     /locus_tag="VBC81_001314"
     CDS             complement(5672..6508)
                     /locus_tag="VBC81_001314"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6912357.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(6483..7154)
                     /locus_tag="VBC81_001315"
     CDS             complement(6483..7154)
                     /locus_tag="VBC81_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6912358.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            7264..7836
                     /locus_tag="VBC81_001316"
     CDS             7264..7836
                     /locus_tag="VBC81_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6912359.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            8013..8987
                     /locus_tag="VBC81_001317"
     CDS             8013..8987
                     /locus_tag="VBC81_001317"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6912360.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            8991..10106
                     /locus_tag="VBC81_001318"
     CDS             8991..10106
                     /locus_tag="VBC81_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6912361.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            10108..10455
                     /locus_tag="VBC81_001319"
     CDS             10108..10455
                     /locus_tag="VBC81_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6912362.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
BASE COUNT         3474 a         2083 c         1661 g         3322 t
ORIGIN      
        1 tcagagaaca taacaattgc aatttactct taataccgtt atcatgttca tatgctaata
       61 cccaagaaaa tagtggcttt tgcctcaaat gaatctgaaa ataatttctt tattacgtta
      121 aagacttact ttaattattt gttttctatt caaatcatta cagatatttc aactctcaat
      181 catgcagatt ttgacggtag ctttgcattc catgatatag aaactagcat acctcatctg
      241 gtaattgact ccaactactt agcgattagt cagactaact caaaaacaga ggctaacgat
      301 attaaaacgt tctcggaact ctccaaaaca atgaccgagt tttactatat gctaaatttt
      361 gacttattta accatctccc atatcgtttt agattgcata ataaggacgg tcaaacaatt
      421 tattcgaacc ataaacctga agatcctttt gatatttatc ctgaagaaga atatcctata
      481 gacaaatggg ttcaaaacag tctaatagag aaaaaagcga aagagctaca tttactctta
      541 cctggcgcat cacaagatta tattctagta caatcttata aacgcttaga aaatgatagt
      601 gggcaactag ttggttatat tgaacatgtc cataatatta aaccactatt agaggggtat
      661 ttaaaagaat ccggtcaagc tatcgttgga tggtctgatg tcacgtctgg tgcttctatc
      721 tctaacgatg attttgaact ataatccact cgtcactcta aaaagaaaac taataactaa
      781 aaggcactgg tttaattcca gcgcctttta ttaatcaatc tccagttgtt gtaagttcaa
      841 ttttttcttt aattggtaga cctcgattag ctgtaaaaat caaaggaact acttctgttg
      901 taacttcgga aaattgtaga ccaaaccatc gtattggtgt tgctgtccaa tgctttacag
      961 atgatttcat tagcataaca aatcgttcac caggttttag gttttgactg cttgaaaaac
     1021 gttcatcaag gacaataaac ctaaaatctc ctactttact atcaggtcta acgctgtatg
     1081 tctttccttg tttaggcaga cgtccacttt caagtaattc ttctacaatc gtacgaagat
     1141 agcgtgacac agtctgacgc atcttaaatc caaggtaaag ttctaccttc acaatgaagt
     1201 cggttcccat catgtcaact ttatactcag ctgtataagg ttcatcagtg accttaatgt
     1261 ttacaaacca ataaacttgt gcttttttag ggcgcttatc aaggatagaa tacatgatag
     1321 acttatcaat catgtctcct tccatacgat ttgtcaaata aacaacgtta gtatgataaa
     1381 tcggataacg atgatcatgt ctaagtttat ctaattgtcc gatgtaatct tttaaatcca
     1441 acaacttaac ataacgcgaa acaattttac tacctttata ccagatagtc atgatgaata
     1501 taattgctac tgctataata acaacaacat agcctccatg cataaattta acagctgaag
     1561 caaggaagaa gattccctct aaaataccaa agaaaatcat catcaataag actaaacctc
     1621 tttttactcc tttttgaatt aaaaagaaag acagtaaaat agttgtcatt agcatcgtaa
     1681 ttgttatcgc taatccatat gctgcttcca tgtgcgctga agtcttaaaa agcaaaacaa
     1741 tagaggttgt aatggcaaat aagaaccaat taataacagg tatgtaggtt tgaccaatat
     1801 tgtcaccagg ataagttgaa cgaaattgtg ggaatatttt tagtcgcata gcttcagaaa
     1861 ctaaggtaaa tgatccagaa atcagtgcct gtgaagcgat gatagctgcc aaagtagcta
     1921 aaataacgac atgcattgta aattgcgaag gaatactagc aaaaaaggga ttcaattcat
     1981 ttcctgcgtt cttattagct aaaatccatg ccccttgccc acaataagaa agtataatgg
     2041 caaccttaac gaacggccat gaaacatgta tatttccacg cccaacatga cctaagtcag
     2101 agtatagtgc ttctgctccc gttgtcgcca ggaaaataga tcctaaaata aaaatacctt
     2161 tatgattctc tggactaaat aacaatttca aaccgtagta tggattaatt gctttgaaaa
     2221 cttctggatg ggcaaaacta ttaaggagac cgctaatacc taaaaaggca aaccatataa
     2281 acataattgg cccaaataat ttcccaataa cacccgttcc aaacctttgg atggcaaaaa
     2341 gtaacagtaa aataaacaag gtcgcaaaaa taacattact ctgattctgg aaaatatgct
     2401 gcaaactagg aactactttt aatccttcaa cggctgaagt tacggttaca gctggcgtca
     2461 aagctccatc tgacaacaag gttgcacctc caataacagc cggaacaatt aaccaaggtg
     2521 tcatttttct aacaagggta tataaagaaa aaataccacc ttcgtggtga ttatccgcct
     2581 ttaaagctac aagcacatac ttgatagttg taataagtgt caaggtccat atgattaaag
     2641 atatagaacc taagataaac gattctgtga cactagaaat accaccttgg ttttcaacca
     2701 atgattgcat cgtatagagt gggcttgtac caatatctcc ataaacaatg cctaaagcaa
     2761 taataaatcc tgcttttgat gctttatcaa aagaagaatg attgacatgt tgcatttctg
     2821 ccaaccctcc gattaaataa tattagtcaa aaaaatggct gaactcatta tagcagatta
     2881 agttaaaaaa acaacactta tctataaaaa gaccagaaat tattaaagcg tttcctggtc
     2941 tttactattt taatcacgat aaacaggttg aggtccataa gtttgactga ctggcattat
     3001 ttctatgcga ttaatattaa tgtgttctgg ttggctagca acccatgaca cggtattagc
     3061 aatatcctct gcttgaattg catgcgcacc ttcataaagt ttttcaactc ttttatgatc
     3121 tcctttgaaa cgtacagttg aaaattctgt cccttcacat aaaccgggtt ctaggttagt
     3181 aactctgatt ttagttccag ctaaatcggc acgcaagttg agagcaaatt gttttacaaa
     3241 agcttttgat gcgccataga cattagctcc tggataagga attgttcctg cagttgatcc
     3301 caaattgata attaaccctc tattaacttc taccattttc ggtaaaatgc atcgtgtcaa
     3361 gtaaattaat cctacaacgt tagtattaat catagtcatc caatcttcaa aatcagcttc
     3421 atatgattta tccaatccaa gtgctaaacc tgcattatta accaacaaat caattgattg
     3481 caaattttta ggcagacttt ctaaagcttt gtcaaccgat tctctacttg taatatccat
     3541 ttaaagtgga taaaagtaac cctctgaaaa cttttctcca aggcatttta atttatccat
     3601 tcgacgagca caaccaataa cgctataacc atctgaaact aatttagtaa caacagctgc
     3661 accaaaacca gcagatgccc ccgtcacaag cgcaacttta cgtgatatca taaatactcc
     3721 tcctctagta tatagaaaaa cgagctagga aaaatccttc tcgttttata acttattttg
     3781 atttctcatc aatagcttga gcagctgtaa tgatagcaag tttgtaaata tcttcagcac
     3841 ttgatccacg tgataaatca tttactggag cattcaatcc ttgtaagatc ggtccgattg
     3901 cttcaaacat acccaaacgt tgtgcaattt tatagccaat atttcctgat tgaaggtctg
     3961 ggaaaataaa aacatttgct tttccagcaa catcactatt aggagcttta atttctgcag
     4021 tttccggaac aaaagctgca tcaaattgta attctccatc aacagctaat tcgggagata
     4081 aatctttggc aattttagct gcttcttgaa ctttttcagc ttgaggcgct tttgcacttc
     4141 cttttgttga aaaactaagc atagcaatct tgggattaat atcaaatatt ttggctgtat
     4201 ccgcagtatt tactgcaatt tctgctagct cttgtgcatt aggatcaatg ttaatagcac
     4261 agtcagcaaa aatataacgt tcttgtgtat tttcacggtt catcaagaat acacctgaag
     4321 tgcgtgaaat tcctggtttt gttttaataa tttgaagagc aggacgtaca gtatcagctg
     4381 ttgaatgaat ggcccctgat accataccat ctgccaaacc aagttttact aacataaccc
     4441 caaaatagtt aacatccttc aaaagacgat ctgcatcttc catagtagct ttgcctttac
     4501 gaatctcaac aaaagcttct ttcatctctt caaatttttc gtattgttca gggtcaataa
     4561 cgctataatc ttgatcaaca aaaccaagtc ttgccaaaga atcgtgaact tcgtcagcct
     4621 tacctaaaat aacaggatcc acaagacctt caaatttcaa acgagctgct gcacgtaata
     4681 cacgttcatc gcttccctca gggaaaacaa tcttaacata atcacttgca actttttctc
     4741 ttaaaccacc aaataaggaa cgcatatata taaaaccttc tatttctaaa ttaatgtaat
     4801 tccatgataa cgctgtcaaa gtcatctgtc aagtctagcg tctgtttatt tatttttccc
     4861 ataaaagggt cgtaaaaaga caaagaatga caatgcaatg cttgcctgtt tattcctaga
     4921 tccatacgtc ctccgtacaa atcatctccc aaaagaggaa agccgatatg agcaaagtga
     4981 actctaattt gatgagtacg ccctgtatgt aatttgatat ccactaatct aacatcacca
     5041 taacgagcaa ctacttgata actagtatgt gcatatttgc ctgattcatg gactcgcctc
     5101 gtaataatac tatcaacatc tcgtgcaata ggagctataa tatcaccact atctgctaaa
     5161 tcaccactcc ctgatactaa tgcataatac cttttttcaa ttgctttagc ctgaagttgt
     5221 ttatctagac gagcatgggc ataaccatgc ttggcgaata acattaatcc acttgtatct
     5281 cgatccaacc gggtcacaat atggacctgc tgattggcat agttattaga cacataatag
     5341 tgttttataa aattagcaat tgtatttgaa tgaatagagc taggaatact agcgaaacca
     5401 aaaggtttat ttaaaatcaa aaaatgatca tcctcataaa caatatctaa atcgtgaggt
     5461 actggttcca aagtttcgtg agaaggctcg tcaggaatat ctatcgtaac aacatcgcca
     5521 atctctaata agaaaatagc gttctgctct tcaccattga caaaaatctt accacctctg
     5581 tacttaatct ttgctaataa cccccttgaa acatcatgac ttttcaaaag tgttttaact
     5641 ttgcaccttt cttctgctat gtattcaaat ttcaatgttc aggttctccg ataaaagcat
     5701 cgttaacacg ttcccaaaag ctagtgtgac ttggagttga aacaaagtta atactttttt
     5761 cgtcaatact atattctatt ttggtaacat ttttgtaatg gaccgttttg ttatctatag
     5821 aaattgtata gacaccaact cgtttaggaa ctatctctat agcatctttt ttggggatga
     5881 taaccgatga ccccaaggta cgatagacgc gattattaag actagaaatt tctgttagtt
     5941 gcaaggcttc tatcgttggg tggagtactg ccccccctag tgatttattg taagcagtac
     6001 taccggttgg tgtagaaact aaaatgccat ctcccctaaa cctttcaaat accacttgat
     6061 taataacaac atccgctacc attgtttttt caatacgctt aatagtagac tcatttaaag
     6121 cacgcgcacg aataactcga ccatcttcaa gggtaatagt tactttcaaa ataggatagg
     6181 agatttgttc acctttatca tttttcaaat tattaattaa tgtgtcaact tcaaaatctc
     6241 gatagtctgt ataaaaccct aaatgtcctg tatgaacacc gacaaacctt actttatcca
     6301 gttgtttttc gtacatatgg aaggctgaca aaagcatacc atctccacca atagaaatga
     6361 caatatcagg atcctttttt gataaataaa aatcaggatc atgtttaaaa gcagcaaaga
     6421 gctttgaggc tacacgcttg ctttgatatt ttccgttagc aattatagca acacgtgttg
     6481 ctctatctgt aaaattcatc tgtgtcatca ctatttccta caccatcgct caattttcga
     6541 tttaaaggat caaataaaag ctgtgcttca cgaatatctt cgcgaatctt acgcatttct
     6601 tcatccaatt ctaaagcgat ttttgctgtt ttctccagtc tttgtttaat ttcttcaggg
     6661 aagtccccct gatacttgta atttaatgaa tgttcgatgg ttgcccaaaa attcatagcc
     6721 agagttctaa tttgtatttc tgctaaaact ttcttttgtc catctattgt gtctacagga
     6781 tactctacaa cgacatgata tgaacgatat ccactagatt tcatatgggt aatatagtct
     6841 cgctcctgaa ccactgtcat gtcatgacgt tttctcaaca aggcaaggac ttcatcaacg
     6901 tcatctacaa attgtaccat gatacgaaga cctgcaatat cttgcaagtc ctgagcaaga
     6961 ttttcttctg aaataccacg taaaaccatc ttttcttgaa tactttcaac cgactttacg
     7021 cgtccagtaa caaattctat tggagagtga cgattttgct ttctaaattg tttacgaata
     7081 ccacgaagct ttatttttaa ttcaccaacc gtttgaatat aagggtctag aaatgtttcc
     7141 caatccattg acattctaat cctccaaaat atttaatgta ctctcttgta aaataaactt
     7201 tttttctata aaattaacac aacaattaat attataccat atttaggaaa aggaagtgat
     7261 aagatgacac acctagaaat tgaatacaaa actctattga ataaagacga atttaatcgt
     7321 ctaacaagct tattttctca tgttcaacct attactcaaa ccaattacta ttttgacaca
     7381 gaaacatttg aaatgaaagc acaccgaatg tcacttcgca ttagaacgtt acctaaccgt
     7441 gccgaactaa cactaaaaat ccctagagaa gtaggaaatt tagagcacaa tcatgacctt
     7501 actttagaag aagctaaata tatcgttaaa aatggccagt tccctgaaga tactgagatt
     7561 gcttctttaa tccttgaaaa gggagttgat ccaaccaaat tagctgtctt tggacaactt
     7621 acaacaacaa gacgagagat ggaaacttca atcggcttaa tggctctaga cagcaatata
     7681 tatgctgata ttaaagatta tgaacttgag ttagaagtca aacaacctaa acaagggaaa
     7741 agagatttcg accagttttt aaaagaaaat aatattaatt ttaaatacgc caaaagtaag
     7801 gtggctagat tctctgctac tttaaaaaat aactaacgtt gagagattgg attttccaat
     7861 ctcttttata attctcattt aagcaagaac ttaaaatctc atactatctc tcctcaacat
     7921 tgttattgca agcattttca tgtttcttat cgtgaatttt ttctattttt ttggtaaact
     7981 attagtatca atctaaagga gtcatctatc tcatggcaga acaatacgcc gataaacaaa
     8041 tcaaactttt ctcattaacc gctaatagag aaattgctga aaaaatttcc caagcttcag
     8101 gaattccttt gggtaagatg tcatcacgtc aattctctga tggcgaaatt atgattaata
     8161 ttgaggaaac ggtgcgcggt gacgatatct atatcatcca atctacaagc ttcccagtca
     8221 atgataatct ctgggaatta ctcattatga ttgacgcttg caaacgtgca agtgctaata
     8281 ctgttaacat cgtagtccct tattttggat actctcgtca ggaccgcatc gctgcatctc
     8341 gtgaacctat tactgcaaaa ttggtcgcta atatgttagt taaagctgga gttgatcgag
     8401 ttcttaccct cgacttacat gcagtgcaag tacaagggtt cttcgatatc ccagttgaca
     8461 atctttttac tgtcccacta tttgctgaac actataatca actcggtctc tctggtgaag
     8521 atgtcgtcgt tgttagtcct aaaaattctg gaatcaagcg tgctcgtagt ttggcagaat
     8581 accttgactc tccaattgct atcattgatt atgcacaaga tgattctgaa cgtgaagaag
     8641 gctatattat tggtgaggta gaaggtaaga aagctattat tattgatgat attttaaata
     8701 caggaaaaac ctttgccgaa gctgcaaaaa ttttagaacg tggtggagca actgagattt
     8761 atgctgttgc tagtcatggg ctttttgctg gcggcgcagc tgatatttta gagtctgcac
     8821 caattagaga aattatcgta acagactctg ttttatctaa agagcgtatt cctagcaata
     8881 ttaaatatct gaccgctagc catctcattg cagacgctat tatccgtatc catgaaagaa
     8941 aacctttaag tccattattt tcatatcgtt cagataagaa ggattaattg atgatttatt
     9001 tagacaatgc tgctactacc gctctaaccc catctgttat tgagaaaatg accaatgtca
     9061 tgacaagtaa ctatggtaat ccatctagta tacatacctt tgggcgtcaa gcaaatcaac
     9121 ttttacgtga atgtcgacaa attattgctg aatatctaaa tgttaattca cgtgaaatta
     9181 ttttcacttc tgggggaact gagagcaaca atacagctat caaagggtat gctcttgcaa
     9241 atcagctaaa aggtaaacat attattacct ctgaaattga acatcattca gtcctacata
     9301 ctatgactta cttatcagag cgatttggtt ttgatattac ttacttaaaa ccaaaccatg
     9361 gacaaattac tgcaaaagac gttcaagaag ctttacgaga tgatactatt atggtatctc
     9421 tcatgtttgc taataatgaa accggggact ttttaccaat tcaagagatt ggtcagcttc
     9481 tcaggaacca ccaagctgtt tttcacgttg atgccgttca agtctttagc aaaatggaac
     9541 ttgatcctca ttctttagga attgactttt tagctgcttc tgcccataaa tttcacggtc
     9601 caaaaggtgt tgggatactt tactgtgctc cccatcactt tgatagtcta cttcatggtg
     9661 gagaccaaga ggaaaaaagg cgtgcttcaa ctgaaaatat aattggtatt gctggaatgt
     9721 ctcaagctct tactgatgct acgactaaca cccttaaaaa ttggactcac attagtcagc
     9781 tgagaacgac ctttttagat gctatttcag accttgactt ctatcttaat aacggtcaag
     9841 actgcttacc tcatgtactt aatataggtt ttcctagaca gaataatggc ttgttattga
     9901 cacagttaga tttagctgga ttcgcagttt caacaggttc tgcatgtact gcaggaacag
     9961 tcgaacctag tcatgtctta acaagcttgt atggagccaa ctcaccacgt ctaaatgaat
    10021 caatacgtat tagtttttca gaactaaata cccaagaaga aattcttgaa ttagctaaaa
    10081 ccttaagaaa aattatagga gattaatatg gcatttgaaa aagaaataaa tcttccagac
    10141 tgcaattaca tttacactat cagcccgaac attaaaaaat ataccttacg cgatactaca
    10201 ttcactcaaa atcgcttagg taattatgaa ttagagcgtc tcctagaaac agtacctaat
    10261 tctggcgatg gctttcctct aaagattaca atcaataaag aactttctgg ttttaagctc
    10321 ttaattactg ataaatctgg ccttagaata gtaaatatct ttaaatctac cgatcataaa
    10381 attcttcaag acaaatttta tttcttaatg gacagcctcg ttgagcgtaa tatctttacg
    10441 aaaactgcaa aataatttac cgaccactat ttttaacatt ttagtggtct ttttgcgaac
    10501 cattttgttg tcactttcac aatctttttg tacaatagag
//
LOCUS       DATRNX010000062        10257 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRNX010000062 DATRNX010000000
VERSION     DATRNX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..2142)
                     /gene="recJ"
                     /locus_tag="VBC81_001320"
     CDS             complement(<1..2142)
                     /gene="recJ"
                     /locus_tag="VBC81_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6912363.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKF"
     gene            complement(2139..2900)
                     /locus_tag="VBC81_001321"
     CDS             complement(2139..2900)
                     /locus_tag="VBC81_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6912364.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            complement(2902..3831)
                     /locus_tag="VBC81_001322"
     CDS             complement(2902..3831)
                     /locus_tag="VBC81_001322"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6912365.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            complement(3873..4520)
                     /locus_tag="VBC81_001323"
     CDS             complement(3873..4520)
                     /locus_tag="VBC81_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6912366.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            complement(4513..5751)
                     /gene="hflX"
                     /locus_tag="VBC81_001324"
     CDS             complement(4513..5751)
                     /gene="hflX"
                     /locus_tag="VBC81_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6912367.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            complement(5842..6732)
                     /gene="miaA"
                     /locus_tag="VBC81_001325"
     CDS             complement(5842..6732)
                     /gene="miaA"
                     /locus_tag="VBC81_001325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6912368.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            6843..7019
                     /locus_tag="VBC81_001326"
     CDS             6843..7019
                     /locus_tag="VBC81_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6912369.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            complement(7061..7979)
                     /locus_tag="VBC81_001327"
                     /pseudo
     CDS             complement(7061..7979)
                     /locus_tag="VBC81_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
     gene            complement(8114..>10257)
                     /locus_tag="VBC81_001328"
     CDS             complement(8114..>10257)
                     /locus_tag="VBC81_001328"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6912370.1"
                     /translation="IKNYTGYYYLYEIKRGKDKVKILDPYAKSLAEWDSNTVNDDIKT
                     AKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFTSDQSLDGKLKNQLGTFA
                     AFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTSSDNNYNWGYDPQSYFAL
                     SGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHTAKTYLFEDIEPNYYHFM
                     NEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDGFRFDMMGDHDVAAIELA
                     YKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTDTVGVFSDDIRNSLKSGF
                     PNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQYIAAHDNLTLHDVIAKSI
                     NKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTKRLLNPDYMTKVSDDKLP
                     NKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHPISTKTQAYTAGLITLRR
                     STDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTIDSKGDIYAVFVNADSKAR
                     NVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSLLIDPLTAIVIKVGKVAP
                     SPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNKTDSYLTNEANLPKTGDK
                     SSKILSVVGISILASLLALLGLSLKRNRHLK"
BASE COUNT         3222 a         2026 c         1536 g         3473 t
ORIGIN      
        1 aaacttaact gtctctttta attcttgata aatttgactc tcagagatat ctcgcttttc
       61 tgcttcttta ttgactgtca taatcccctc tgtaatcgta acaaaatgta gttcttcaaa
      121 aatttgaatc attttaatta ataaaatatc aggaatatga aggtaggaac tgagctcttt
      181 taacttataa cgaacgtcaa attctgggaa ttgatatatt gttttatata aactagcaaa
      241 ttgatcacgt gtaccataac cagatataaa ataatttttt ggtatctcat ttttaaaata
      301 gatagcatca aactgtttac cttgagtcat ttgttttaat tcttgtggat gatcgggaac
      361 agtcaaaaga ataacttctt tgctctgctc ttcttgagac aggattggca ctccatgagg
      421 taagctactt gctttgcttc taatatcaaa taattgaata ccatctacac gcgcatcctc
      481 caacattaat tgtagggttg tggcaccatt ccattgatta acagataaag tgacagctaa
      541 ctccaaatgt tgtgcttgtt gaaactcttg tagttgactc cccatattaa atgctaccaa
      601 atcaagtgct tgtccatctt gttctagttt taatttcaga tgagcaccgt tttgccccat
      661 gactctggct tgactcactt taaaatcttt taacaaaaag actggttttt tattatccat
      721 tccaaatggc gctaactttt caaaatctct aacagtgtct aaactaagtt cgtcaaaacg
      781 tagctcacta tcaattgtca gagttttttt ctgactcatg tccaattgtt ttttagagat
      841 atagtcacat aaaacttgag aaagatcacc taccttactt tcctctaaag tcatgcctgc
      901 tgctccgctg tgacctccaa aagcaatgaa taattctcga tgctggtcaa aagcttgaaa
      961 aatatcaagt gcttctacac ttctggcact tcctttagca ataccatctt caatattcaa
     1021 aacaataaca ggttgtccag ttctctctaa aatacgaccc gccacaatac ctaaaacccc
     1081 tggatgccaa ttttcttttg ctaaaacttg tacaggcttt gtttgatcaa gcatttgcat
     1141 agcttggtca aagatagttt gaacgatttc tttgcgctct tcattttttt gatcaatcat
     1201 ttgggctatt gcttgagatt cttcatcatc aaaccctgtt agtaattcaa tagctggatt
     1261 agggtcatca agtcttccta aagcgtttaa ttgtggagca attttaaatc caatagtttc
     1321 ttcatttagt gtttttaaat caatatttga taaagatatt agctcctgta agcctattct
     1381 ttcactatct ttcataactt caagtccagc ttttaccatg atacgatttt catcagttag
     1441 gctaaccata tctgcaatcg tcccaatagc aaccaaatcc aacatttctg tcgggattgt
     1501 ttctaagaga gcacaagcta ctttgaaagc aacaccacac cctgctaaat aaggaaatgg
     1561 ataattagca tcaggatgtt ctgggtgaat aatagcataa gcacaaggta aatctgctgg
     1621 catactgtga tgatcagtaa ctaccacgtc cacaccttga ttttgagcat atgcaattgc
     1681 ttcatgccct gccacaccat tatcaaccgt aatgattaat gaaacatctt gttgttcaat
     1741 aaaatattta taaacacttt gattgggacc gtaaccatct gtaaagcgat ttgggagata
     1801 aacctggact tcagctccca tcatatctaa tgcttctttc atgattgatg ctgatgtcat
     1861 tccatcagca tcatagtctc cataaactaa aatagtttca ttattttcaa tagctcgtct
     1921 aatacgatta acagctttat ccatatcatt taacaaataa ggatcatgga gattttccaa
     1981 attagtttgt aaaaaatgat gtaattccgc tgatgtctta atacctcgcg aataaattaa
     2041 ggaagccacc gcttcactta tcttttcttt ttttgaagcc tcaaaaaagc cagcgtctgg
     2101 cttttggtta ttgaggaccc aactatactt agcagatatc atttataatt gaataccttt
     2161 ctggcaaaat aatcagctgt ttttggaaat aaactataat atttatgcgc aaaagctaat
     2221 ataaaaggaa gatttaattc tctcttattt tttccaagaa tagaaactac ttttttagct
     2281 actttttctg gtgttagaac aaaacgacct atacttgcta aataatcacc acttggatca
     2341 gcctgagcaa aaaatcctgt tttgattggt ccagggttta cacttgtcac ataaacattt
     2401 ttttctgcta attctaaacg aagagcattc aaaaatccaa cgacagcaaa cttactagcc
     2461 ccataaacgc tagctttact tgttgctatg aggccagcca tgcttgcaat attaacgata
     2521 tgaccagatt ttacaagaga catcttatgt cctatctgac gagctataga catcgttgca
     2581 aatgtattca cagcaaacat gtctttgact tcttcatcac tataatccca aaattcttta
     2641 aaagaaccaa atccagcatt attaattaaa atatcaattt gaccaaattc aacataaatt
     2701 ttttcaatca tattcgttac agcattgtca ttagttatgt ctagtgataa acataaggtg
     2761 tttggtctct ttccataaag cttttcaagt ttttctcttg atctccctgt gacaattaaa
     2821 tggtcgtctt gaggtaattg gttaataatt gcctgtgcta aaccaccaga agctcctgta
     2881 atcagtatcg ttcttgccat tttatatacc tacttcttcc aaatctctaa caagatgggt
     2941 atttgaaaaa atcgtctttg catcttcttc catttgccaa caatctcgcc ccataaaacg
     3001 agcactaaca tgatttaata atagtcgttt agcatttgct tgtttagcga tatcagctgc
     3061 ttgcatattg gttgaatgcc catgcgattt agcaattcgc tcatcaccct taccataagt
     3121 agattcgtgc actaaaacgt cagcgcccaa agctaaccta atacttgcat ctgtttttct
     3181 agtatctccc aaaatagtga ttactttacc ctttttcggt tcagaaatat aatctttagc
     3241 aataatttct ctaccatctt ctaaggtaac attctcacca tttttgactt tcccaaaaag
     3301 gggaccaaat ggaacacctg ctagtttcaa tgcttcagca tctaaagtgc cctcaaggtc
     3361 tttttgcaca acacgatatc ccatacaaaa gatagtatga tctagctttt ctgcataaac
     3421 ggtaaattta tctgtttcca taatttttcc gagtgatgat tcatcaaact cgtgaaaatg
     3481 aattcgataa ggtaaacgtg aacctgatgt ccttaagcca gtcataacaa aactttttat
     3541 accgaccggt ccataaatat ctaaatctgt ctgttcttca ttggcttgaa aagcccgact
     3601 ggataaaaaa cctggaagac caaaaacatg gtctccgtgc atgtgcgtga taaaaatttt
     3661 tttgacttta cgaggtttaa tcgtggtttc taaaatttga cgctgtgtac cctctccaca
     3721 atcaaacatc caaacctcgt taatttcatc taataatttc aagactaaac ttgaaacatt
     3781 acgagctttc gctggttgac cagctccagt tcctaaaaat tgaatttcca tacttgttga
     3841 cttttttata atccttccat aacttaataa atttaattag tagatttagt atcaatataa
     3901 ataactgact cacgtccttg actagaataa tagtattcac ctgactttgt tttaaccaac
     3961 tctaatagct cttcttgatt gaaactagtt attttaataa tatctttttt aagttgtaag
     4021 acattcccta gtaaacttgt ttctggtgta atattttgag attctgtttc gtcaaaatct
     4081 atttttgcca ttctgatacc agaccgatct tgatagactt tatattgagg aaaaatctgt
     4141 gcaatttcta agagtaactg cggtgtaatg acttctttaa cttcagaaaa aacactcgct
     4201 tcttcttgat catttaggta agcaaaacca aatgattttc ctgataaatt caaacgaata
     4261 aaaggttttt cttcatcaaa cgataaatgt tttggaaaca agcccaaggc tttggaaaga
     4321 tcaaaatagt aatcagttgc cgcttctggc ccttgctttt gatatatttc agcaaaataa
     4381 gcattaatca cactaggagg tggagtgata aaatcaagtc gttgttgctt agagaggcca
     4441 tctagttttt tctctagata aactttatct aagctcgtaa aaccaccata agtcaaagcc
     4501 aaatcaatat aatcaatcat aaaattcttc cagcttccat ttttgtttag gagaaatata
     4561 gcctgtaata ttctcgtatt cttcttcaaa ggtgtaggtg tcaagcaatg ctattttatt
     4621 gagatcatag agcttatatg cttgattttg atgcactcta atcgaaaatt cgtcaaagat
     4681 atgtcgtatt tcatccacaa taagacggcg taataaatcc ctactccctt gttttttggc
     4741 tgcaattctc acattaggaa aagttgttgc attcagctgc tcagtgacat ccatcttgtt
     4801 gtatatcgct aaacgaggaa tatctatcat atctaagtct ttaagaatct ccataacaac
     4861 tttttcgtgt tcttcatgat tagggtcact agcatcaata acatgaagta ataaatcaac
     4921 atgtcgactt tcttctaagg tagacttgaa agccgcaact aactctgtag gtaaatcttg
     4981 aataaaacca acagtatctg ttaaagtgac ttgaaattga ttttgtaaat agatctgctt
     5041 ggttgtagcg tctaacgttg caaataattc attggcttca tattgcttat catcggtcaa
     5101 aacattcata atagttgact tgccagcatt tgtatatcca attaatccaa ttttaaaggt
     5161 tgtcgaatca acacgtcttt ctcgaactgt ctcgcgattt ttttctacaa tcttcaattg
     5221 tctttcgata tcagaaattt gatgacgaat ggaacgacga ttcaactcta actgagattc
     5281 tcctggacct cggcttccga ttcctcctgc ttgacgacta agcataatac cttgtccaac
     5341 taagcgtggt aacatatatt tcaattgcgc tagatgaacc tgtaattttc cttcatgact
     5401 tctagcacgc atagcaaaga tatctaaaat aagttgcata cggtcaataa ctttaacacc
     5461 aagttcagct tccaaattag agttttgtcg cggagtcaaa cgattattaa ccacaacggt
     5521 atcaatctca tctgcttcta caatagcttt tatctcctct aatttaccag atccgataaa
     5581 tgatttacta tcatatttat ctcttttttg ataataatga ttaacaacat ttgctcctgc
     5641 tgtcttagct aagctagcaa gttcttccat ggacatttca aaattctcag tatcttgaag
     5701 ttctacccca actaaaataa cacgttcttg ctcttcttta gtttctatca cctataatct
     5761 cctttcaata tttttttctt gttctaaata cgtaatcttt attataacat aatgcccttt
     5821 aaatgcgaga aacggcttta tttactttga tgataaaaaa tcactgactt ttcgcttgat
     5881 tttttgttga taattttcct caccaaccat aataaattcg acattcatac ggtttctaaa
     5941 ccatgttaat tggcgttttg caaaacgtct ggtattttgt tttagtttat caacagcttc
     6001 ttctaatggt atttgctttg aaaagtaagg aaataattct ttatagccaa ttcctctact
     6061 tgcttgaaca ctagggtagt tatcgtatag ccatttagct tcatctaaga gaccattgtc
     6121 tatcatcaaa tcaactcgtc tattgatacg atcatagagc acctgacgat catcattcaa
     6181 tccaattaat aaaacatcat acggactttc actattttgc aaatcattcc caaacttcgc
     6241 caattctaat gcacggatgg cacgtcgacg attaacttgc ggtataacaa tggattgttc
     6301 tgtcaacttt tcaaataaat cttcatcact tagagactct aatttttcac gataggcctt
     6361 aagagtttct tgattgactt cgcctcctaa atgatagccc tcaaacaaac tctgtaaata
     6421 taggccagta cctccaacaa taatagggat tttcccctta gaaataatcg tatcaatagc
     6481 tacttttgcc tctttgacaa aatcataaac cgaataattc tcatttactt cacgaacatc
     6541 aatcaagtga tgataagctt gttcttgctc ttctttacta gctttagccg ttccgatatc
     6601 taacttctga taaacttgct gactatcgcc actaatgatt tcgccattga aagttttagc
     6661 taattcaatt cctaatgctg tcttcccaac cgcagttgga ccaacaactg ctataagctt
     6721 gatttttctc atgatacttc tcaattctat aaaggttttt acttgaaatt taactagtat
     6781 ttcgttacca taattatatc atagatagaa gcagagtctt catcatagat tggagaatta
     6841 ttatggttaa aaaattcgca tttgctaaag gaatagcaac aggagtcgtt gcaacagctg
     6901 ctactcttgc aggtgctgca tttgctatca aaaaaacaat tattgaacct gaggaagaaa
     6961 aaatagcttt tatcgaagaa aatcgtaaaa aagctgcacg taaacgtgtc tctcattaaa
     7021 caataactcc cttactagct agtaagggag ttattttata ttaaattgtt cgccttaaaa
     7081 agaaaatata acgtaccatt actatcataa caataagagt tgttgtccca atttcaaagt
     7141 ggactagccc ctgtatatca actggtaatg atagatgctg aatacttaat ttaagtgagg
     7201 tagctgagat aacaaggggg aaagtaaagg ctgagaaacc tggtgtaaaa tcaccaatca
     7261 gtaatttagg cacctgaaaa agaatgaaaa aataaagaaa ttgtgccatt acgattaata
     7321 gcaataataa acctctattt ggtgacacaa aactattaac atatcccgct gtgattagcg
     7381 acattggagc gcaaatagta gttatattag gcttaactgc cgaaggtagc ccgattttat
     7441 atgctttaat gaatagaaat ggtaataata ctaaagttgc aataaaacca taccaaaaaa
     7501 ctattttccc aatagtgaat tgtccactaa ttggtgctgt caggctagct acccctatac
     7561 caacatagag gacagaccaa gaaggaaaaa gattagccat cgaaaagtgt ctcaaatatt
     7621 tccatgaaaa aatagccata tgaatgatta acgcaataaa tgacaaccac catacacctt
     7681 tttgacagaa ctgctaccca tattcctaaa cttctaaata ataagatata ggtcgataaa
     7741 atcatacctg ccataaaaaa agtagtaaag acagaagcaa tcaaaggatt gcttaactcc
     7801 ttcctgcttt cttttttatt ttttaatatt cctttgatta aaaaaatcca aaaaacaagt
     7861 gctactagcc ctaaacaata gcgaaaatag gtgccatatc cctctagaag gttccccaaa
     7921 gctaatagcc ccaatactaa tcccgccatc actaatggtg gtttttccca atttttcatc
     7981 atatacctca taaaaaaacg tttacatcca ttataacaca attagatttt gctgataagt
     8041 gattttatta acttaatgaa aagaagctag cccattgact aacttctttt ttaagctctt
     8101 ctaaagtttg cttttatttt aagtgacgat tcctctttaa agagagacct agtagagcaa
     8161 gtagacttgc tagaatgctt attcctacta cacttagtat ttttgatgac ttatctccag
     8221 tttttggcaa attagcttca tttgtcagat atgaatcggt cttattagaa actacaggag
     8281 ttattgaggt cttattagct attctattta ctttttctac cgttttagat cccttgaaag
     8341 attgtgtttt gggataatct gcttgcaatt cctccttagg gctaggagca actttgccaa
     8401 ctttaatcac aattgctgtt aatggatcaa tcagcaaact atctttttca aaatgaacac
     8461 ctctaggagt tgagattggt ttaatccccg cttgatcagc atcaacaatt acttgccctt
     8521 ttaaaaggtg tttatatttt tcacctagta aaacgtttct agctttacta tcagcattaa
     8581 caaatactgc gtaaatatcg cctttagaat ctattgtttg gtaagcaata accaaatctt
     8641 tttctttaat atcaccttga cctacctctg taatcaagct aacctcacga tcaatttctg
     8701 ctttgctcaa tttccggaaa gcatctgttg aacgccttaa tgtgattaaa cctgctgtat
     8761 aggcctgtgt tttcgttgaa attgggtgtt tgttattatc tgtggctgct gcccaatcaa
     8821 aatgattaat ggcatctgaa gaatcatatg aatcatgaat aaaatatggg tattctttaa
     8881 cagcttcaat aagtgttgct ttattaggca atttgtcatc tgaaactttt gtcatgtaat
     8941 cagggttaag taaacgcttc gtacgaccat actcttgacc agaatgaatg aatgctgtcc
     9001 cttgagatgt taaaatcatt acatttccta aacgcagacg tctatgaata tcttcttcag
     9061 ctaccttagg gtctttatta attgattttg caatcacatc atgcaaggta aggttatcat
     9121 gtgcagcaat atactgcacc acatctcctg gcgaatctgc ttcaaaattc ccaggttgtg
     9181 ctttgatatt tttaaaaata ccttgtaaag attgtgggcc acctgtgatg aaagctggag
     9241 taccttcatt tggaaaacca gatttcaagc tattacgaat atcatctgaa aagacgccaa
     9301 ctgtatcggt tgacttcatc caatcttggt cagctggttt aaccggctta ccttgatcgc
     9361 cttggaatgt tctccagccc tcaccaatca taatcatatt aggattaata gctttagctt
     9421 ctttataagc taattcaatc gcagccacat catgatctcc catcatatca aaacggaaac
     9481 catcaacttt aaattcactt gtaagatatt taatggaatc aaccaaaaca cgacgactca
     9541 ttgcatgagt ggttcctaaa cgtccccctc caaaactttc tcttggtgaa ccatcttcat
     9601 tcataaagtg ataataatta ggttctatat cctcaaagag ataagttttt gcagtgtgat
     9661 tatagacgac atcaagtata acccccatgc cacgtttatg aatatcatgt attaattgtt
     9721 ttaattcggc gatacgtgct gatggatctt ttggtttctc tgaatacatt ccagaaagag
     9781 caaaatagct ctgtgggtca tagccccaat tgtaattatt gtctgaggaa gtgtaagctg
     9841 ttgagcgtga cttatccatt tcattaacat aaaaataact caataccggt aaaagctgaa
     9901 tgtgtgtaac tcctaatttc tgtaaataat ctagtttctc tgaaaaggct gcaaaggtac
     9961 caagttgatt ttttaatttt ccgtccaaag attgatcaga agtgaagtct cttacatgtg
    10021 cttcgtatat aacagcatct tgtcttcctt taaaattagc aattttagca aaacttaaat
    10081 tttgaggtcc aagttgactt ggatttacaa aagctgcttt agccgttttt atatcgtcat
    10141 taacagtatt actatcccac tctgctaatg actttgcata aggatctaaa atcttaacct
    10201 tatccttacc tctttttatt tcgtaaagat aatagtaacc agtatagttt ttaatac
//
LOCUS       DATRNX010000063        10202 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRNX010000063 DATRNX010000000
VERSION     DATRNX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            41..1042
                     /locus_tag="VBC81_001329"
     CDS             41..1042
                     /locus_tag="VBC81_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912371.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(1114..1728)
                     /locus_tag="VBC81_001330"
     CDS             complement(1114..1728)
                     /locus_tag="VBC81_001330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6912372.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(1755..2438)
                     /locus_tag="VBC81_001331"
     CDS             complement(1755..2438)
                     /locus_tag="VBC81_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6912373.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQPRSVA
                     LSFNYFMLN"
     gene            2550..3713
                     /locus_tag="VBC81_001332"
     CDS             2550..3713
                     /locus_tag="VBC81_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912374.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            complement(3759..4742)
                     /locus_tag="VBC81_001333"
     CDS             complement(3759..4742)
                     /locus_tag="VBC81_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6912375.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            4890..6785
                     /locus_tag="VBC81_001334"
     CDS             4890..6785
                     /locus_tag="VBC81_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6912376.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(6835..7788)
                     /locus_tag="VBC81_001335"
     CDS             complement(6835..7788)
                     /locus_tag="VBC81_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6912377.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            7955..8191
                     /locus_tag="VBC81_001336"
     CDS             7955..8191
                     /locus_tag="VBC81_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912378.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            8193..>10202
                     /locus_tag="VBC81_001337"
     CDS             8193..>10202
                     /locus_tag="VBC81_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6912379.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELES"
BASE COUNT         3184 a         1619 c         2000 g         3399 t
ORIGIN      
        1 taaaaaaata aaatcagtaa aattagaaag gaggtttgtt atgtctaaaa aaatgaccat
       61 taatgacatc gctcaattat caaaaacttc caaaacaacg gtttcttttt tcttgaatca
      121 aaaatttgaa aaaatgtcgg atgagacgag gcagcgtatt caagaggtga ttgatgaaac
      181 aggttataga ccaagtacga tagcaagaag tctaaattct aaaaaaacaa aactactagg
      241 tgtcttaatt ggagatataa caaacacatt ttcaaatcaa attgtaaaag gaattgagca
      301 catcaccaaa caaaaaggct atcaaattat tgtgggtaat agtaattatg atgccaaaag
      361 tgaagaagat tatattgaaa atatgttaaa tttaggtgtt gatggtttca ttattcagcc
      421 tacatcaaat tttcgaaagt attctcgtat tttaaaagaa aaaaagaaac caatggtatt
      481 ttttgatagt caactttatg aacataagac gagttgggtt aaggctaata actatgatgc
      541 tgtttatgat atgacacagg agtgtctaaa tcgagggtat aaaaagttta ttatgattac
      601 tgctgatacg agcttgctta gtacacgtat tgagagagct tcgggattca tggatgcttt
      661 aaaggacaat ggtttcggtt atgacacttt ggtgattgaa gatgatgatc attcaaaaag
      721 tgatatagaa gactttctaa aagcggtagt tccagataag gaggagacat tagtatttgc
      781 accaaattgt tgggctttac caatggtttt tactgccatg aaaaatctca attttgatat
      841 gccacgagtt ggtttagttg ggtttgacaa tattgaatgg acagactttt catctcctaa
      901 agtgtcaaca attgtccagc cggcttatga agaaggtgaa caagtagcac agattttgat
      961 taatcgtatt gaaggagatg actccgtcga taaccaacag attgtagatt gccagatgtt
     1021 ttggaaagag tcgacttttt aaaaaaagat ttcttatttt atttgctatt aaaaaataaa
     1081 acctagagaa aattatctct aggttatttg tccttattct aaaataagta agccttcttt
     1141 gacagcgaaa ggatttttct cattgatacg gtcgtaaaac ataataccat caatgtggtc
     1201 aatttcatgt tgaacaacaa tagagttgta accttttaat ttgagtcgat gtttttcacc
     1261 agttttgtcg aaatattcaa tagttacacg cgcgtggcgg acgacatagc caggtacttc
     1321 acgatctact gataggcatc cctccccgtc agataaagca gcatcttgaa cagaatgcga
     1381 tacaacttta gggttgtaca ttacttcttg gaggctataa gcttctttag gtggatttcc
     1441 ttgagcatcc tcaacgtttg gaacaaggac ggcaatgatg cgttttgaga tgtctaattg
     1501 tggtgcggct aaaccaacac caccacgtaa gcctagcttc tcagccataa ttggatcttg
     1561 agagtgtttt aaaaattgca tcattttttc tccgagaatt tcttctttct cagaaagtgg
     1621 gaatgtgact tcttcagcca ctttgcgaag ggttggattg ccttcgcgga taatgtcatt
     1681 catgtcaatt aaatgactag cttttactaa tttatcaata gcagacatat tttctcctaa
     1741 ctgtattctc tgatttagtt tagcataaaa taattaaatg ataaagctac gcttcttggc
     1801 tgaatattta atgattctag tgcgaaacga tttttgataa agtattttgg tttttctttt
     1861 tggataaggc cttctttgac catatcatgg ataactctta ataaatgacg ataactgatg
     1921 ccaaacgaat cagctaaact acttagttca aggtgaaagc aaccttgttc ttcaattgct
     1981 aaaatatgac ttgcaagtaa ctctttgacg gtatacccta aattagtaga cgctttgatg
     2041 ttttgatcat gaaaagcctg agctagctct tgactaagtt ttaggaggaa agttgcgtct
     2101 gttaacagtt tctctttccg tcctttaagt gataattgaa tgactgtaat atcctctaag
     2161 gcaataacag atgatacaat ttgtctatta gtcaaaagtt caatatcacc aatcagtgtt
     2221 ggttttgttt taatatctaa aatatgttct ttgccgttaa agagtcgacg tacaatttta
     2281 aaacgtccct tgacgatgta gtgcaagtat tctaattgtt ctccctgttt acagacggat
     2341 tggccggcag ttagggtgat tacttgtaaa tccttgaaat aatgttttgg aaagatagtc
     2401 tccaaacagt agtcttggca gtagttttct atatccatgt attgactccc tatttgagat
     2461 agttttagga catatgtcat atatttctag tttaatattg attatacttt atttatctaa
     2521 aaaaagacat cagaaagagg tattgtcact tgaaaatctt tatggagaag ctaagtttac
     2581 tctctttatc acttatcttg ctatcaactt tttcgacatc accagcccta ccacaaatga
     2641 tatcttatta tagagataag ggcttaccat caccccaagt tgaattatta ttttcaattc
     2701 cttcgatggc tattatcttc atcttgctta tcacaccttg gttaagtaaa aaactttctg
     2761 agaaacatat gattatcttt ggactattgc taacagcatt agggggagga ttgccggttg
     2821 tcagtcagaa ctacctttta gtatttgtat cgcgtctttt attggggagt ggtatcggtt
     2881 ttattaatgc tagggccatt agtgtgatta gtgaatatta tcaaggcaaa gaaagaagaa
     2941 aactcttagg attaaggggg tcctttgaag ttttaggaaa tgctggtttg acagccttag
     3001 ttgggctatt gttgactttt ggttggtcta aatcatttat gatttatttt ttagctttac
     3061 caatcttagt tctatatctt gtttttgcgc ctaagaaagt ggtgaaagat acaaatgaca
     3121 agattaaaac taaaggtcaa aaaatcccta aagctgattt gacttatatt gtcgccttag
     3181 ctattttagc aggatttgtc ataactataa atactgggat taatcttagg atacctctgc
     3241 tagtagtaga atttggtttg ggtacgccag ctcaagctag tcttgttctt agtgcgatga
     3301 tgcttatggg aataatagca ggtatgagtt ttggtcagtt gatagctatg tttcataaac
     3361 aattaattcc aatctgcctc gtcttatttt ctttaacatt gcttggagtt ggattgcctt
     3421 ctaatctaat ggtactaaca attagtgcga tggcgtcagg tttcctttac agcctgatgg
     3481 tcactgctgt gtttagccta gtagcagacc gtgttgaata ttcgttggtt ggttctgcaa
     3541 cgacattagt tttagtattt tgtaatattg gtggagctag tgcggctatt cttctgtctt
     3601 gttttgatca tttactagga caaataaatg cagttttcta tgtttatgct attttaagtt
     3661 tagcagttgg tatgatttat ttccttaaac gcctattttt taagaaaatg tagtgtatat
     3721 taactaaaaa gggcttgaca atcattgtca agctctcttc atttttcttc ttctgggtag
     3781 gtaaatcccc attcttgacg tagctgtgtc atgatatcca taacatcttt ggtatagttt
     3841 aagtacatgt ggttagtttt tcctgaaatg gcttcttcca tatcagctac ctcatattgg
     3901 agagcatttt cagttttgcc agcttcgata atatcttgat gcccatcctc agtataagta
     3961 ataactgcct tttgtcctcg cggatattca aaaagttcaa tgtagccttt atcgtaagcg
     4021 atagttgctc gtttaggttg ttttgcatgt aaactaaggc tgacagtcgc catctcattt
     4081 gctgggttgg ttagtaggat accaacttgt tcatcaaccc ctgttggtgc aaatgtaact
     4141 tgagaggtaa tgttgtgagg tgcttctgac ataaaccagc gaatacaaga aagtgcataa
     4201 acaccaatgt ccagcaaagc accgcctgct aggtcacgac tgaaaaaacg gttagtcata
     4261 tcatattctt tataacttcc gaaattcatt tgaatcattt ttaacggtcc taattttcca
     4321 ctatcaacta atgtttttaa ttggcggtaa attggcatat gaaaaatagt catggcttca
     4381 gctaagacaa catggttagt ttcggctaaa tctatggctt ctttaagctc agtactattt
     4441 aaagtaatag atttttcgca aagaacgtgc ttaccatttg ctaaagcctt tcgtaaaaat
     4501 gagatgtgag tattgtgggg agtagagata taaatgatat ccacttcagg gtcttcaaat
     4561 acttgatcta tgtgatcata aactttttgg ataccatatt tgttagcaaa ttcaagtcct
     4621 ttgtcgtaag ttctattagc tacagaatat aatttttgtc cacgtgcttc cagtgcttgg
     4681 gctaattcgt tggcaataac gccggttcca agcgttgccc aattataata tttttcagtc
     4741 ataacaacct ccttgttggt ttatagtata gcaaaaataa caaaatgata tttaaagaaa
     4801 gcaccaatat gagagtattt ttaatagtca aactctgaat tttctgttag actgtaagag
     4861 attacatatg agatattaag gagaaaataa tgataaaata tcaagatgat ttttaccaag
     4921 cggttaatgg ggaatgggct aagacagctg ttattcctga tgacaaacca aggacaggtg
     4981 gtttttctga tcttgctgat gacattgaag cattaatgct gtcaacgaca gataagtggc
     5041 tggctgatga aaataaacca agtgatacga ttttaaatca ttttattgct tttcataaaa
     5101 tgacagctga ttatcaaaaa cgtgaagagg taggagtatc accagtatta ccattaattg
     5161 aagaatataa aggtctacaa tctttctctg agtttgcatc aaaagttgca gaatatgaac
     5221 tagaaggtaa accaaatgaa tttccatttg gtgtggctcc tgactttatg aatgctcaat
     5281 tgaatgtact ttgggctgaa gcgcctggga ttattttacc ggatacaact tattatagtg
     5341 aagataatga aaaaggaaag gaactccttg ctctttggcg taaaagtcaa gaagacttac
     5401 tgcctttgtt tggcctttca gaacaggaaa ttaaagatat cttggataag gttttagctt
     5461 tggatgcaaa attggcacaa tatgtcttat ctcgcgaaga gagttcggaa tatgttaaac
     5521 tttaccatcc ttataactgg gaagatttca caaaattagc acctgaactt ccattagatg
     5581 ctattttcca aaagatatta ggacaaaaac cagataaagt tattgttcct gaagaacgtt
     5641 tctggacaga atttgcatct gactattact cagaatctaa ttgggaatta ttaaaggcag
     5701 atcttatttt atcagctgcg aatgcctata atgcttactt aactgatgat attcgtatca
     5761 aatcgggagt ttacagtcga gctttatcag gcacaccaca agcaatggat aagaaaaaag
     5821 cagcttacta ccttgcttca ggaccttaca atcaagctct aggtttgtgg tatgcagggg
     5881 aaaaattctc tccagaagct aaagctgatg ttgagcataa gattgcaacg atgattgatg
     5941 tctataagtc acgcttagaa aaggcggatt ggcttgcaca gtcaacacgg gaaaaagcta
     6001 ttgtgaaact taatgttatc acgccacaca ttggataccc tgagaaacta ccagagactt
     6061 atactaaaaa aatcattgat cctaaactat cgcttgtcga aaatgctact aatttagaca
     6121 aaatttcaat agcctatggt tggagtaaat ggaataaacc agttgataga agtgaatggc
     6181 atatgcctgc ccacatggtc aatgcgtatt atgatccaca acaaaaccaa attgtcttcc
     6241 cagcagccat tttacaagca ccattttatg ctttagagca aagttcatca gcaaattatg
     6301 ggggaattgg tgcagtgatt gcacatgaaa tatcacacgc ttttgacact aatggagctt
     6361 catttgatga acatggaagt ttgaataatt ggtggactga tgaagatttt aaagctttca
     6421 aaaaacttac agataaagta gttgagcagt ttgatggact ggaatcttat ggcgcaaaag
     6481 tcaatggtaa actaacagta tcagaaaatg ttgcagattt aggtggagtt gcttgtgctt
     6541 tagaagctgc gcagcgtgag tcagattttt cagcgaggga tttctttatt aactttgcga
     6601 caatttggcg tatgaaggca cgtgatgagt atatgcaaat gttagctagt gtagatgttc
     6661 acgccccagc acaatggcga actaatatta cagtaacgaa ttttgaagaa ttccataaag
     6721 agtttgatgt taaagatggt gataacatgt ggcgtccagt tgaaaaacgt gttattattt
     6781 ggtaactcta aatgataaac tcccgttact cagatgagta gcaggagtat ttttttattc
     6841 ttttatagat aaagataatg tagtagtgtc aacgcttatc tcagctccaa tagtcactgt
     6901 caaaagaggt tgggtgtgcg caaaatccat atcataaata acaggaattt ccttgaaaat
     6961 agcatgtttt gaaaggatat attcaaagac ttgtggagtc atgccgcatt ctttaggaaa
     7021 gcgccccata agaatagctt gtggatgagg ataagcttgt aaaattgcag caaggttgcg
     7081 gtcaaattca taaaaattac tttcctcagc ctcttcaatc aagaggacat agcgttcaat
     7141 tttcggtgca taaggggtac ctcgaagtag gccaaacgta gagagattac ctccgataat
     7201 cgtaccggaa gcttttccgt gattataaat tttccattct gttgggaaaa attggcgtgg
     7261 tttgctggga tcataccagg gatcactaga ccattcttca ctaggccaaa gttcatagtg
     7321 gttttctgtc atggcagtaa gccaagcttg tgtttggtaa ggttgacctt ccttcatttt
     7381 gaaagaagaa taagcagggc ccatgtaagt ttggattttt gctttggcaa aaatagcatt
     7441 aaggaaggcg gttgaatctg agtaaccaca aattattttg ggattcttag agattaaatc
     7501 ataatctaaa tatggtaaaa gctcgttgga attgaatccg ccaatggttg ctaatatggc
     7561 atcaacagaa gggtcggcaa atgcttcatg taaatcttct acgcggctag caatcgaagc
     7621 agaatagagg atatcatttt ctagatagtg cttagagaat gacacttgaa atcccagctt
     7681 ttctagtact tctttggcta ataggttagc ctcaaaacca cccagacgtt caatggaaga
     7741 agagggacta agaacacgaa tgtggctgcc ttttgttaat tttttcattg gttaacctcc
     7801 tatttaaatt gttgttagtc taccacatat ggagataatg agcaacgata ttcttaaatt
     7861 ctatcaattt aagaaatgcg tgttatacta gagatagccg atgaaagaaa aaatacttat
     7921 tattaataat ttggttatta aataggaggt catgatgtct caattatctt tggaagaacg
     7981 attgaatcga ctaaaagata aatacctttc ttctaaaaat caagaatttt caaatgttca
     8041 cttaagtaaa gtaaagaaga aaaagaagaa attaataata cttgaaaaag aaagatgtcg
     8101 ctgtcgattg gagagagaag atactaaaaa aatcgatgat aaaattgaga aattgaagaa
     8161 agaattgata gatgttatga aggaggaata atatggaaga aactattcta atagtcagct
     8221 ttctcctgtt tttgatttta tctaatgtta tcaataggat atttcctaaa ttaccattac
     8281 catttatcca gttggtcttt gggattctct ctggcttagt ttttcataag tcacaagtcc
     8341 atatcgatcc tgagttattt cttgcctttg ttattgctcc tttgaacttt agggaaggtc
     8401 aagaaagtga tattggtagt tttgtaaagt atcggactat tattctttac ttaatcttac
     8461 caacggtatt tttaacagct attgtcgttg gttatgtagc agggcacctt ttgccagtta
     8521 gtttaccact tgcggcatgc tttgcgcttg gagcagcctt aggcccaaca gatgccgttg
     8581 cctttatatc gattgctaag aggtttcaat ttcctaaacg agcagagaat atcctcaaat
     8641 tggaaggtct tttaaatgac gctagtggtc tagtttcctt ccagtttgct ttaacagccc
     8701 ttgtaacagg ttatttctcc ttagcaaaag ctagtctcaa gttagcattg gccataatgg
     8761 gtggtttttt aattggcttg ctttttgctt ttctaatgag gttatgtctc actgtcctag
     8821 agaaatttga tgctgctgat gttacagggg ctctactttt ggaattaacc ttaccttttg
     8881 ttgcttattt cgttgctgat ttgttgggat tttcagctat tattgcggtg gtagtggcag
     8941 gagtaatgca agcaaatcgt ttgaaaaaag tgactctctt tgatgctcaa gtagatcgcg
     9001 ttacctctgt tatttgggaa accttaaatt ttattttaaa tggtttagtt ttcttaatat
     9061 ttggaagaga actgatacgt atcattggtc ctcttttaac aagtaacgct tatagtaatt
     9121 ttgatcttat tagtattgtt gttttagtta cttgcacact ttttctagtt cgctttctag
     9181 cagtttctcg cttttatgct tggagatctt tcaaatacca taaatcattt aaaaagtatt
     9241 ggagagagat tcaactgttg actttttcag gagttaaagg aagtgtttcc atagcaacaa
     9301 tactcttatt acccaagcac tcagtcatag gagagttggg gtatagtttg attcttttca
     9361 ctgttggtgc agtaacgtta atgagttttt taacgggact tttagtatta ccaaagttag
     9421 cgcctccctt acaggtgaaa gatgattatt taatacgtct atcgatttta acaaaagtat
     9481 tatctgtctt agaagaagac ggtaagagtt ctgaaaatca agcttcattt tatgcagtta
     9541 tagataatta taatagccgt attcgtcatc tcattttaga acaagaatct agtgacatta
     9601 agaaggactt agctgaattg caactgatga tgcttagtat agaaagcgac gggcttgaag
     9661 ctgcttatcg ttatggcaat atttctatca aagaatatcg tatttaccag cgttatttaa
     9721 aatacttaga acgtcaagtt aatcgtagct ttgtgtcaaa tatcacttat agtttaacga
     9781 ttcttatgcg tgttattcgt ggtattgttc atgagttttt aacatttgga caaggaatac
     9841 gaaatcggaa aaaacatttc aaaaacagga aacggttgtt agcagaagtc aatcgtcagc
     9901 acttaacaga tttgtactta tcaaataatg acctcatttt agaaacgcta gaagatatgg
     9961 aaggttatta taatgcaact ttgatagatt tcttaaaaaa tgaacgcatt cgagacgcag
    10021 aattaattaa atcaggttta tttgtagaac gtgtcattgc acgcataacg ccagatattt
    10081 taaatgaaac cctaagagga tattacttgg aacgcagaac aatctctgaa tttgaaaggg
    10141 acggtttaat tagttctaaa gagtctcaaa ttttaaggga taatgtcaat gaattagaaa
    10201 gc
//
LOCUS       DATRNX010000064        10170 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRNX010000064 DATRNX010000000
VERSION     DATRNX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(215..2191)
                     /gene="ftsH"
                     /locus_tag="VBC81_001338"
     CDS             complement(215..2191)
                     /gene="ftsH"
                     /locus_tag="VBC81_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6912380.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2214..2756)
                     /gene="hpt"
                     /locus_tag="VBC81_001339"
     CDS             complement(2214..2756)
                     /gene="hpt"
                     /locus_tag="VBC81_001339"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6912381.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2761..4035)
                     /gene="tilS"
                     /locus_tag="VBC81_001340"
     CDS             complement(2761..4035)
                     /gene="tilS"
                     /locus_tag="VBC81_001340"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6912382.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            complement(4037..5323)
                     /locus_tag="VBC81_001341"
     CDS             complement(4037..5323)
                     /locus_tag="VBC81_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6912383.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(5323..5457)
                     /locus_tag="VBC81_001342"
     CDS             complement(5323..5457)
                     /locus_tag="VBC81_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6912384.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            complement(5460..5831)
                     /locus_tag="VBC81_001343"
     CDS             complement(5460..5831)
                     /locus_tag="VBC81_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6912385.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(5818..6090)
                     /locus_tag="VBC81_001344"
     CDS             complement(5818..6090)
                     /locus_tag="VBC81_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6912386.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            complement(6381..9878)
                     /gene="mfd"
                     /locus_tag="VBC81_001345"
     CDS             complement(6381..9878)
                     /gene="mfd"
                     /locus_tag="VBC81_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6912387.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            complement(9875..>10170)
                     /locus_tag="VBC81_001346"
     CDS             complement(9875..>10170)
                     /locus_tag="VBC81_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6912388.1"
                     /translation="LDMEVGKIRFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRP
                     NGRMTVINHVLGKFDKNDEIMISNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
BASE COUNT         3141 a         1939 c         1452 g         3638 t
ORIGIN      
        1 attttttctt ttatttttaa aaagttaata ttatttatag taactaacct tctatacttg
       61 tttaatggat agcattttta ccgttgtcat gttcatattt catcttctta attcacaaat
      121 ttaaacttca tcttcataaa aaacacccct caaattttat ctaaatttga agggtgtttg
      181 aaatttataa agttctttaa aaatatatga tgacttattt tttatcttct tcttgcattt
      241 tttctttgat ttcatcgtat gataacgctc tcgctttatc ttcatcattt tctgtctcag
      301 gcattttacc tgtctcgaaa atcgatttaa tctgagcagc atcaagagtc tcatatttta
      361 agagggcttc tgcaattaat ttatgagtat cacggttttc gttgataata tcagctgcct
      421 tattacgtgc ttcattaaga aggtgacgaa cttcatcatc aataagttgt gcagtttgag
      481 cagaatatga tttttcaggt gacatttggc cagccatcat tgcatggtta ccttcgtatt
      541 gaactgggcc aagtttctca ctcataccat attcagttac catagcgcgg gccatggcag
      601 tggcttgttc aaagtcattt gaggcacctg ttgtctgagc gttgaaaata atttcttccg
      661 ctacacgtcc tcccataaga cctgctaatt gctctttcat atcatctttt gaaagaagca
      721 tttgatcttc tttaggtaaa gcaatcatat atccacctgc acgaccacgt ggtacgatag
      781 taactttatg aacaactcgc gcatttgaaa gaatcaaacc gacaattgtg tgcccagctt
      841 catggtaagc aaccatagct ctttctcttt cagaaatagt acgatctttt ttagaaggac
      901 cagcaattac acggtcttct gcttcatcaa tatctgaagc atcaataact tttttatttc
      961 gtcgcgcagc aactaaagca gcttcattga gaacattctc caaatcagca ccaacaaatc
     1021 ctggggtttg ttgagctact acttttaagt caacattatc tgctaatggt ttatttttag
     1081 catgtactct cagaattgct tcacgacctt ttacatcagg ttgtccaact agaacttttc
     1141 tatcaaaacg tcctggacgc agtaatgcgg gatctaacac gtcactacgg ttggtagcag
     1201 cgataacgat aatactttcg tttccttcaa aaccatccat ttcaattaaa agttggttga
     1261 gcgtttgttc acgttcatcg tttccgccac ccataccagc tccacgtcga cgtccaacag
     1321 catcaatttc atcaatgaaa ataatagcac gttcagcttt tttagcatct tcaaacaaag
     1381 agcgtactcg actagctcca acaccgacaa acatttcaac aaaatctgaa ccggaaatac
     1441 taaagaatgg aacccctgct tcaccggcaa cagcttttgc taatagggtt ttacctgtcc
     1501 ctggaggtcc ttctaataga acccctgctg gaattcgggc acctagagat ttatatcgtt
     1561 taggatcttt aaggaagtca acgacttcta ttaattcttg cttttcttcc tctgctccag
     1621 caacatccga aaatcgaact ttaacctcat ctttactaga tgatcttgct ttatttttac
     1681 caaagctcat ggcaccacga gcgccaccgc caccgccttg gttcatcatc atcataaaga
     1741 aaccaatcat gataactaaa ggtaaaaaac tggcaatata agaaatccat gtcccacttg
     1801 aactttcatg tttgacctga atattagtgt tattttcttc agcagcgcta acaagagatt
     1861 ttattgaaga gtcatttggt agtataacag aattaaatcc tgtaacttta gtagcaacac
     1921 taccacctaa aaaagtaaaa ctattagctg atttaattgt tttggctttc ttatatgtcc
     1981 cactaacttc aacaacacca ccacttggct ggtatgaaat agacttaatt tctccagctt
     2041 ttaattgttt aacaagtttt gtataagaaa tttgctggtt ttgagagcta gttcctttta
     2101 aatagtattg aaaggttgta ataaccgcaa taatcaataa tatgtaaata aaggaatttt
     2161 tcagaaaacc attattttta ttatttttca tagtttaaga gagtaccttt tttctatttt
     2221 gagtaaattt cttcttttaa cacaccgaca taaggtaggt tacgatagtt ttcagcatag
     2281 tctaaaccga atcccacaat aaattcattt gggatatcgt aacaaacgta atcagcatca
     2341 atatctacca atcgcccttc tggcttatca aaaagggttg caactttgac agagttagct
     2401 tgacgatact taaacatatc tcgaaggtat tttaatgtac gtccagtatc aatgatgtct
     2461 tcaataaaga ttacatctct tccttcaatg ttagtatcaa catcttttaa aattttaact
     2521 tctccactac tagttgtacc accatgatag cttgaaacaa ccataaaatc aatttcaaca
     2581 tgtgtatcaa tatgctttaa tagttcagcc ataaaaggca ctgacccctt taatacacct
     2641 actaataatg gattcttacc tgcataatct gcagttaatt tagctcctaa ttctttcgtt
     2701 ttcagaataa tatcttcttc tgaatataaa acttttttta tatcattttc aagcatgatg
     2761 tcaccttttt tctaaatttt caatataaag tttgcccttc attatatcat gttcagaagg
     2821 ttttctcaaa tatgttctac ctgcaacaat cacaaatatc aactctttat tttgctcccc
     2881 aataacagct tcttctctgt cttttagagt tattttctca tcaataaata gacggcgaat
     2941 ttttttagtg tgatttccta aaaatatcct atccccttct tttcttcttc ttaaaataat
     3001 cggagaagta tcataaatag gaatagtaat gtctgcttga gaaatgaatt ttccatagga
     3061 aaataaatat tgtgagaata tttgtatatt atgcaattct aatctttttt caaccttaaa
     3121 actatccgtc ttaggatgga tttttgtaat atgaaaactc tcgtaatcct tatgaagaaa
     3181 atagcccgat ttaatagtat agtcaatatt ggctttagtt ctaatcaatt ttaatattac
     3241 tctgaactgt tctttactca cttgtaaatc tggaaatttg ctaatgtaat cttgcaataa
     3301 aaattcttgg acggaatgag tttgccttaa aaaatcatag cagtttgtta cttcgatgct
     3361 ccttgttagg tctgttaaag ccgtgaataa acactctgtt tcctcagaga gagttatgag
     3421 agaatctttc aattgattat tttctctttc caaagtagga aagtagatgt ttcttattcg
     3481 atttctcaaa taatcagacg aagcattgct agcatcttca aaatgaaaaa tatttggtaa
     3541 taactccttt ttatatggaa gaaatggtct aatcaattga ccatttgcaa aagcactcac
     3601 ttgcttaatc cctgataaat agcgtagcct gcttcctctt aaaattctca taaatacagt
     3661 ttcagcttga tcatcagcat ggtgagctgt tactaaagcc gtgtaacctt cttcacgcat
     3721 aacttttgaa aaaaaattgt agcgatggtt acgtgctcta tcttctgaaa atattccttg
     3781 aaaataagag ataaagacag ggacatcatg tatctgtccc cactgtctga tatattcttc
     3841 ttctttttct gattcttttc gctgtccatg atttatgtgt gcaataccaa tactaattga
     3901 taactctttt tgatactggt ataaaaattg caacaaattt attgaatcaa tgccaccaga
     3961 aacagcaatt agaactttct gatgagcggt aaataaccct ttggataagg tatctttcaa
     4021 aatagtgtta tacatattac tttaaaacct ggtaaacgtc gtcagcaatc ttagtgatat
     4081 catcgtaaga tgatttatct gtaaaaataa tcataataaa aggttgttct gcataaacaa
     4141 tagccgcatc atgtttgtag tcataagcat ctccaatttt atgtgctact ttaactggta
     4201 tatttttagg gattcttgta ttatcaaaat cagtctttga taggtaatta acaatatctc
     4261 cattttgata ataaattgct tccatcattt tacccgcagc ttgtggagaa atctggcgtt
     4321 tcttcatatc ccagtgcata ccagaaattg tgtctacttg tgtttgaaac attgaatcgt
     4381 attgattatt gacataataa cctagcatat ttgttgcaac attatctgat tccttggcag
     4441 ttgcttcaag taactcctta actgtatata atttatgatc agcttttttc tctaatttcc
     4501 cactaccact tggatcatag ctattaggaa attgattaac attatctttg tattcaaatt
     4561 tgctgtccaa tgacagtttt cctaatttta cttgctcttg gctagcataa agtgttgcta
     4621 attttgcaat acttgcagag tacattgacc tatcaatatt aataccagct actgtctgtg
     4681 tatttaattg tttgatgtat ataccatatt tatctttatt atatttttcc aataatactt
     4741 cttgaacctt agccatacga ttatcagcaa ccgatagatc ttctactgat atccaaaaac
     4801 cactgtccgt tttataataa ataccatgtt ctgtttctgc ttgttctact aaatgcactc
     4861 tggaataagg ttttaaatta tttttaactt cactaacacc atttgtgtaa ggtgcttcat
     4921 aagtcacaaa agaaggtttt aaccaataat ccaatgagac agattgctgc ttagaaacta
     4981 aatcattaaa aatagcttca cgactagcct ttatgaactg tccattggac aaagtaaata
     5041 ctggaattcc atttgaatta attgtaaggg aaataatcga caataaagta tttggaacta
     5101 aagtctctcc tgttatggtt aaagcttcat cttgaaaact gcttatttca atttttgtag
     5161 ttggtctctc tggaatagca tcaaaataca tttccttatc cacaactgtc tgagttaagg
     5221 cgtatcgtct attttgagaa agcgttaatg gctcctcggt acttatagct gggattgata
     5281 caaaatataa gctacataat aagaaggtta aaactttctt cattactttt ctctgctctc
     5341 aatcttttct ttttctacaa ttgtttgcct aagttgatag ttcttatcag ctaactcttc
     5401 tagtttgtca tccgagtagg ctagaaaatg ttctattgtt tcaattaaat tattcataat
     5461 tattttggta aaaggtctgg taatgggtaa atcatttcgc cggtcttaga gaaataatac
     5521 ttcgctcgag catatttttg aacgtaatct ggatttttta gttgttttgc tagtaacttc
     5581 tggttctcag ttctattagt taatgtctga tagtctttcg ttaattttac aacttcttga
     5641 cgacgttctt gtaaagttct gtaactctta actaaattat aagtgggtaa aataaataaa
     5701 agcatgacaa aaataagaac ccaacccatt aaacgatttt ttcggcgtaa ctcctcagct
     5761 tcgtaacgtt tttttagatt ctcatcgtta atatattgat tatttaactg aacaacatta
     5821 ggcttgctca tctgtttcta tccttgtttc attaataatt tcatacattt tgatagcatc
     5881 ttctttctta gtgctatctt tcatttctaa tactttaacg gtcaacaatt tattaccaaa
     5941 acgaatttca acttggtcat taagctttag gtcagttgat gactttgcta atacgccatt
     6001 caccttgaca cgtcctttgt ctgcaacttc tttagcaacg ggacgacgct taatgatacg
     6061 cgacactttt agatacttgt ctaatctcac tgatgatcac atccttctat ctaaattaac
     6121 tctactattt tagcattttt tttgaaaaat atctgaactg ataatatttg tcaatataat
     6181 gtaacaaaaa gagagctatt agctctctta attctctcaa atatcaagta aaaaacttct
     6241 attttaataa cctctaaaaa tatttatgtc tttcaaaagt tgagatgttt gacgtgaaac
     6301 ataatgtttc tcgtactatt cttcttgaac caaacatttt ctaaaagagt atccaaattt
     6361 ccttgcaact tttcacttag ttattcttct actggtttac gtgacttaat ctcgataaaa
     6421 cattcggcaa attttagaag ttcttcaata atttcataat ttttcttatg tttgatatta
     6481 aaaacaactt ccatcttacc atttgtttcg ctaattctag ccttaagttg agtttttgat
     6541 agtgcttcaa aataatcttg agttaaaaaa tatttcaaag atgctttttc aaatatgatg
     6601 cttatttcat taccttttct ttctactaat tcagtaaagg caagatccag ataagctttt
     6661 aaaagtccta tttctaaaag gtatgcaact tgatcaggat attctccaaa acgatctatt
     6721 aactcatctt gtaaggcttc ataatcagcc ctagtttcta attcacgaat acgcttatat
     6781 atttctattt tttggcgctc atctgatata tattctgctg gtaaataggc gtcaatttgt
     6841 aaggctaatt caacattacc tttttgacga attaatgatt ttccttgttt ggtggcaatc
     6901 gcttgctcta gaagctgtga atacatctcg aatccaaccg aatcaataaa accactttgt
     6961 gaagctccta aaatatttcc tgcaccacgt atagataaat cacgcatcgc tattttaaaa
     7021 ccagatccca attctgtaaa ccctttgatt gcatctagtc ttttctcaga aatttctgtt
     7081 aaaactttat caggtctgta cataaggtaa gcataagcaa tgcgattact tctacccaca
     7141 cggcctctta attgataaag ggttgacaag cccatatgat ctgcattttc aacaaagagg
     7201 gtattaacat ttgaaatatc gactcctgtc tcaattattg tcgttgcaac taaaacatcg
     7261 taatctccat tgataaagtc tattaatgta ttttctaatt gaatttcaga catttgacca
     7321 tgaacaaaac caatactagc ttctggtact aactcctgaa gttctgaaac tttctgttca
     7381 atagtgtcaa ctttattgta aacataaaaa acctgtcctc ctctgtctat ctcacgaata
     7441 atagcttctc taactaaacc aggatttgtc tccaaaacgt aagtctgcac aggataacga
     7501 ttagttggag gtgtttcaat gactgataag tctctaatgc caagcattga catatgtaat
     7561 gttctaggta ttggggtcgc tgtcaaagtt aagacatcaa cttttgtttt taattctttt
     7621 aacttttctt tatgctttac accaaatcgc tgttcctcat caataactat taaccctaaa
     7681 tccgaaaata caacatcttg agagagaagc cgatgtgttc caataataat atcaacttgg
     7741 cccttagata atctttttag tgtatctgtt tgttcttttt tgctacgaaa acgactaagt
     7801 acatcaactg taacaggata attactaaaa cgttctttaa aattttcaaa atgctgctga
     7861 gctagaactg ttgttgggac taaaacagca acttgtttat ggtcattgac agctttgaaa
     7921 gccgcacgca tagcaacctc tgtcttacca aaaccaacgt ctccaaccaa taatcgatcc
     7981 atcggacgat taccttccat atcctgttta atttctttta tcgaccgcaa ttgatcctct
     8041 gtctctacat atgcaaaatc attatcaaag tcattttgca tattatcgtc aggagaaaat
     8101 gcaaatcctt gtaattgact acgctctgca taaagtttca acaaatcatc agcaatatct
     8161 tctacttgct ttgcaactct ctgttttgct tttttgaagc gaccatcatt taacgtatta
     8221 attttaggct ctttgccatc tgcagagaca tattttgtta gaagttcaat ttgttctacg
     8281 gggatagaga tccgatcagc attttgatat tgaatagtca gataatcacg atgaatacct
     8341 tgtatctcaa ttgtttcaat acctaaaaat tttcctacgc catgaacatt atgaacaaca
     8401 taatcgccaa ctgaaagttc attataatct tttagacgtt ctgcattact aatattactg
     8461 cgtctaattt tccttttaac acgcctatga taaatctcac gttcagtaat cagaacaatt
     8521 ttctcatctg caaaataaaa accatttgaa agatgcccaa caataagttg tgactccttt
     8581 ggaacgatat cgtttttatc tgaaatgatt aaatctaacc catattcttt taagttttct
     8641 tgaaggagat ttaaaccttt ttgagaatct acttgcaaca gtactgtcgc tccagatttc
     8701 ttataacgat tgatttcatc gaccaacaat ggaaattgat taaaaaattc ttgcattcca
     8761 tattgagtaa aatgatgaag tttatcaaat ttcagatttc ctaatccttt gtggaaattt
     8821 gaaaagaagg ttgctggttg gtattgtctt aattgcttat aaggatctga aaaatagttc
     8881 aaactagaat gagacttgcc ttgttgtaaa tcttccgtca gaaggctagc tatttctaaa
     8941 tctaacttag catttcgatc tacaattttt tgaaaatcat caacaaaaag tggagttccc
     9001 tcaggaatat agtctaatat tccccattct ttttcataaa agatacttag aaaacgacga
     9061 atatttgagt gcttaaaatg attctcggtt gtagataaaa cctcgctcaa ataggcgcgt
     9121 tgttcctcac tagctgttac tagatagccc tcaagttttt tctttgcacg ctcgaaatct
     9181 gcatcttgta aaataatatc atctgctggc gaaatctgta ctgactctaa ttgtttaaga
     9241 gacttttgag tgtcaatatc aaactgcctg atgccatcta tttcatctcc gaagaattcc
     9301 aagcgataag gatattcttg tgtcatctca aaaatatcta aaatatctcc tctttggcta
     9361 aattcaccag gactaaatac ttgagaaact ttctgataac ctaaattgac taagttctta
     9421 ctcaatttat ctaaataaca ttcttgaccg atttcaaact tatattgact tttcgagaaa
     9481 acttccggat ttggtaatag taaccttaaa ccactgatac tagtaatcaa aacaccgttt
     9541 ttttctggat cttttagaaa cctcaaggca ctgagtcgtg aaattgattt gtctaatgaa
     9601 gagaaaataa actctgctgc aggaacgtca tcagcaaaaa attgataaac gttatcttct
     9661 ccaattaaac ttgatatatc acttgataat ttatcagaat cgttttgagt ggcagtgact
     9721 accataattt ttttagataa cttctcgtag gctgatgcta tagcaatagc tttacttgct
     9781 cctgaaaacc ccataactaa ttggcgacta ttagttacta atccagagtg ccatgtccgc
     9841 accactttat tttggctaaa taattctata atattcattt taaaccgttg tatttctgca
     9901 ttgttttttg aaaatcattc gtctgtaaat aataattgac agcattgtca accttatcaa
     9961 gagtatttga tatcataatt tcatcatttt tatcaaactt ccctagaaca tgattaataa
    10021 ctgtcatacg tccattcgga cgtcctatac ctactttaat acgatcaaat tcttgagtac
    10081 ctaaatgagc aataatagac ttgataccat tatgtccccc agcagatcct ttttgacgaa
    10141 aacgtatttt accgacttcc atgtctaaat
//
LOCUS       DATRNX010000065        10124 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRNX010000065 DATRNX010000000
VERSION     DATRNX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            41..1105
                     /locus_tag="VBC81_001347"
     CDS             41..1105
                     /locus_tag="VBC81_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6912389.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            complement(1213..2520)
                     /gene="eno"
                     /locus_tag="VBC81_001348"
     CDS             complement(1213..2520)
                     /gene="eno"
                     /locus_tag="VBC81_001348"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6912390.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            2718..3173
                     /locus_tag="VBC81_001349"
     CDS             2718..3173
                     /locus_tag="VBC81_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6912391.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            3186..3671
                     /locus_tag="VBC81_001350"
     CDS             3186..3671
                     /locus_tag="VBC81_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6912392.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(3692..4333)
                     /gene="serB"
                     /locus_tag="VBC81_001351"
     CDS             complement(3692..4333)
                     /gene="serB"
                     /locus_tag="VBC81_001351"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6912393.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(4427..6151)
                     /gene="ezrA"
                     /locus_tag="VBC81_001352"
     CDS             complement(4427..6151)
                     /gene="ezrA"
                     /locus_tag="VBC81_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6912394.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KAQERIRF"
     gene            complement(6245..8197)
                     /gene="gyrB"
                     /locus_tag="VBC81_001353"
     CDS             complement(6245..8197)
                     /gene="gyrB"
                     /locus_tag="VBC81_001353"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6912395.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(8198..8758)
                     /locus_tag="VBC81_001354"
     CDS             complement(8198..8758)
                     /locus_tag="VBC81_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6912396.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(8877..10082)
                     /locus_tag="VBC81_001355"
     CDS             complement(8877..10082)
                     /locus_tag="VBC81_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6912397.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT         3289 a         2023 c         1609 g         3203 t
ORIGIN      
        1 ataaacagct actattttat gttattttag aggttaacag atgaaaagaa aacagtttat
       61 aaaattagga attgcaacct tactaacggt tatttcgctt tacacaccaa taaacctagc
      121 tacaaatcat accacagaaa atattgttac tgctcaagag tataaaacaa aagagaatgg
      181 tactttacct tttaaacaca aacgacaatt ggttttagga gagttggatg acaaaggtcg
      241 tgccacattc gcacatattc agctaaaagt taaagatgaa cccaagaaaa agagagtaaa
      301 acgtttaaaa acaacccctg ttggatggca caattttaaa ttctattata atgatggtac
      361 tcaaaaagct tggctaatga gccgaggccg tcttatttgc caccaattta gtggactaaa
      421 caatgaaaga aagaatttag ttccaatgac gaattggcta aatactggaa actacaatag
      481 cacgaattca tctaaccctg agagcatgct attctatgaa aaacaactaa agacttggct
      541 ttcgactcat aaaaactact acttagatta taaagtaaca ccaatttatc aaaataatga
      601 actaattcca agaaaaattg aattaaaata tgttggtatt gataagactg ggaaattatt
      661 acctattttt atcggaaaca aatccactca agatcaattc ggcatcagca ctgttacctt
      721 agagaatacc tcaccaaatg ctaccattga ctacctcagc ggaaaggctc aaaatactgt
      781 cttatcagct aaagaacaaa gaaaacttat cgctaagcac gaagaagaaa aacgacttgc
      841 agaaaagaaa gttgaagagg aaaaagctgc tgctgaaact cagaaaaaac tggaagaaga
      901 acaagcaagg cttgctgctg aagctcaaag gaagcaaaaa gaggagcaag caaggctcgc
      961 agctgaaact cagaaaaaac aagaaactct agtacaagaa caaactagtc aaggctacaa
     1021 acgtgactat agaggaaggt ggcatcgtcc aaatggacaa tacgcttcaa aagcagaaat
     1081 cgctgctgct ggattgcaat ggtagaactg tttattatca aatcaattct attattataa
     1141 tgtgtacata aaaaccatct tgcatatgca agatggtttt tattatattt tcaactgttt
     1201 aataatctga gactattttt ttaagttgta gaatgatttg atacctttgt attgagcaac
     1261 ttcaccaagt tggtcttcga tacgaagtaa ttggttgtat ttagcaatac ggtcagtacg
     1321 tgataatgaa cctgttttaa tttgaccagc gttagttgca actgcgatat cagcgattgt
     1381 tgaatcttca gtttcacctg aacggtgtga tacaactgca gtgtaaccag cttctttagc
     1441 catttcaata gcttcgaaag tttctgtcaa agtaccgatt tggttaactt tgataaggat
     1501 tgagttagca gcttcttctt tgataccacg tgctaagtaa tcagtatttg taacgaagaa
     1561 atcgtcacca actaattgta cacgtccacc aaggcgttct gtaagagctt tccaaccatc
     1621 ccagtcgttt tcatccatac catcttcaat agtgatgatt gggtatttgt taaccaactc
     1681 ttcaaggtaa tcgatttgtt ctgcagcagt acgaactgct ccaccttcac cttcgaattt
     1741 actgtagtcg tatactttac gttctgcatc gtagaactct gatgaagcac agtcaaaacc
     1801 aatcatgatt ccgttttcgc cagcttcgta accagctgct tcaatagctt taagaatagt
     1861 ttctacgcca tcttcagtac cttcaaattt aggtgcaaat ccaccttcat caccaacagc
     1921 tgtttcaaga ccacgttctt taaggatttt cttaagagca tggaatactt cagcacccca
     1981 acgaagtgct tctttgaaag ttggtgcacc aactggcata atcatgaatt cttggaatgc
     2041 gattggagcg tctgagtgag aaccaccgtt gatgatgttc atcattggag ttggaagaac
     2101 tttagtgttg aatccaccaa ggtagctgta aagtggtact tcaaggtaat cagctgcagc
     2161 acgtgctacg gcaatagaaa caccaagaat agcatttgca cctaatttac ctttattagg
     2221 agttccgtca agagcgatca tagcacggtc gatagcttgt tggtcgcgta catcataacc
     2281 aatgattgct tctgcaataa cgttatttac attgtcaaca gctttttgtg ttccaagacc
     2341 accgtaacga gacttgtcgc cgtcgcgaag ttcaactgct tcgtgttcac cagtagaagc
     2401 tcctgaagga accataccac gtccgaaagc acctgattct gtataaactt ctacttcaag
     2461 tgttgggtta ccgcgtgagt caaggacttc gcgtgcgtaa acatcagtaa taattgacat
     2521 tacaatactc tccttatgag ttttaatttt ttacatattc ataatatcat aatctttagt
     2581 taataacaag gaaaaaccca gtgaaaccgc tttattacgt ggtttttgat aaaaaaatca
     2641 ctaattcttt tcaagtttac ctgtgctaat gcaacccatt taggttataa taaaattagc
     2701 aaattcaagg agaaattatg actgaacttg acaataaaat cctacaaaaa gcaagcggtg
     2761 aaaatcgtct tgatcctgat caacaacgtt actaccttgg tacttataaa gaaagagttg
     2821 tgctccactt aagtttaaaa gaggcacaag aagaaaagtt tcaacttgct ataacatctg
     2881 ctcttaataa tctgatactt gaatatccta atcttagttt aaaattatca ccaaatctcc
     2941 ctactgatgt tcaaatgttc tacatgaagt tagctaaccg tctatcacta gctgctacga
     3001 ttgttaatga agcaaatgcc aattctccat ttggtattat tatacatagt gataaggctg
     3061 aaaacgttga atggaacgat tttgagacac aattccctga tttattcaat tcaccaaaaa
     3121 aagaggagtc tcctaaaaaa agtctatggc aacatttctt tagtcaaaaa taacaaggag
     3181 gtctaatgga aatttgggat gcttatgacc aatatgggtt cattacaggt cttacactta
     3241 atagggatca aaatatccca caaggcttat tccacttagt tgttgatgtt atcctttttc
     3301 atgaggatgg tgacgtattg atgatgaaac ggcaccctaa aaagaaagct tttcctgctt
     3361 atttcgaagc caccgctggc ggatctgccc ttaaaggaga aaatgctaag caagctattc
     3421 taagggaact caaagaagaa actggtatcg ttcctcaatg tctaaccttt cttaatagag
     3481 agtggttctc tgagcgttct tactttgtag accattttat cgcaaaatac aatggtgcaa
     3541 aagatataat tacattgcaa gaaggagaga ccgttgacta tatctggtta aaaccagaat
     3601 atattgattt atttttatct aaaaacaaat taataccctc tcaaataaaa ctcttaaaat
     3661 ccttaatcta aaaactaggc ttaataaagg cctagttttc atttgcatca ataatatcaa
     3721 ataccttaag caaatttttt tcattaatct gatacgcagc ttcttctctg acaattggtt
     3781 ttgcacaaaa agcaattcct accccagcgg atttgatcat tggtaaatca ttggcaccat
     3841 ctcccattgc gatggtttgt gaaaggtcta agtgattttc agaagcccac tcttttaact
     3901 ttattttttt cacttcttta gtgacaatct ctccctcaac ttcgccagtg agaaaaccac
     3961 ctttgacacc aagacgattc gctttgacat aatcaacttg taattgctta gctaagatat
     4021 caatcgtctc gtgaaaacct cctgatacaa gaccaacttt catctgacgc ttgtgacact
     4081 ctttaatcaa ttcataagcc cctggagtaa aatgtattga ggataatata tctgtcaaaa
     4141 tagtaacagg caaaccttta agtagcgcta ctcttttctt caaagcttct tcaaaatcta
     4201 actcgccacg cattgcagcg tcagtcagtg cagcaacctg tttccccacc cctgcttcaa
     4261 tagctaataa atcaatagct tcttccatta ttaaggttga atcaacgtcc atgactaaca
     4321 aacctttaat catttaaaac ctccaaaact tttttactat tgtaccttaa aattagcacc
     4381 tttcatagat agcaattaaa aaaagaagag tactaactct tcttttttag aaacgaatac
     4441 gttcttgtgc cttttcataa gacgtcacaa aacggtcagt tacacctggt tccacagtct
     4501 caagtgcgta tgaaatttca tcgaaagatg ctttatagtt atgttcaact tcaaataagt
     4561 acaaagcttg ttcaaagctc ttttgcacat tttgttcaaa actacgataa cggtttgaat
     4621 actgtagtaa ctgctctgtg agagtcgcat cttgaacaac tagatatgta gcttgctcta
     4681 aattagcgat agcatttgta gtcacatcat ttaaacgtga aacagcctca atatcaatac
     4741 gaccgcgact gagttcatta ataagtgcct caatttgaga acttgttgta aagaaagtgc
     4801 ttaagaagtc ttgcgggatt cctggaagat tgcgtttttc catgaaacgt ttaatcatat
     4861 ggagcttgtt aacatactta tccatatttt ggcgtgctcg tgtctcaacg ctttcaatag
     4921 ttttaacggc ttcaaaaact tccatttgat tttcttcaac agtagtcaat gttttcaacg
     4981 tacgctcaaa taaaatttgt agatgagtat aaggtgaagc ttcttgacca aaattttcta
     5041 cagaaggtaa aacctttgtt tcaatttctt ccaaatcatt tgtgaaggaa cgtaaactta
     5101 agctttcatt ttcatttaag atatatttag cagataatcg tttgatttca tgccccagtt
     5161 gttcattgtt agattttgca tgagcaagga attgaggaat aattttacta tccttatgag
     5221 ctactttata agcagcaatc tcacgttcaa aaatatcata aagtttatca attttttctt
     5281 gaatatgttc attttcatcg cgagcacgat ctagatccaa actaactaaa ccatctgaat
     5341 tcgaacgaat agcctcacga acttcttgaa aacgttgttc aatatccttt tcagggaaat
     5401 ggtagttttc ctctaaaaga cgacgatatc ctgtctccaa atcatctaac tgatctggaa
     5461 aatcgtcttc taatttagca acaatagctg gaatttgttc tgtaatttgc cctaaagcaa
     5521 tcgtatgctc ctctgcttta ttcaaaactt cagaagcttc aatcgggtca ccagttgagt
     5581 ttaaggtcac aaaatgtgaa aattcagcct cgatattttt caactgcttt tcaatttctg
     5641 gcatggttgt cccgtaatta tcttcttttt cagaaattga tttttgaagt gtttcgtata
     5701 ggtccaaagc atgtgttaca cgggcactat ttttctcttc ttgctctttt aaaacttcta
     5761 aagcttcacg aatcgaaaca atatcttctt ctataatagt tagctgacta tcaacattat
     5821 caatctcgtg cttagcacgc acaaaattaa atgaatcatt aagattttca gcttcaacta
     5881 aatgaaaatc aatatcttta aatgaattag ttgataaatc agtccatttt tggttccatt
     5941 ctctaaaagt tgattgactt tggccaatca aatgaagcaa tttgacttgc tcaatctcct
     6001 cttgcacagg aaggtcaact aactcttgtt ttctagtttc taagtttgca attaatgtat
     6061 cattacgttt tcttattacg acgccgacta cgtaggcaat aatcacaagc aggactatag
     6121 ccacaagcag caatatgatt ccgctcgaca ttcaactctc cttataatgg tctaattttc
     6181 taaaaatcta ctataacgtt aagattatag catacttgaa ctgtttttca aagctatctt
     6241 tggactagat atctaagtta ctataaacag cattagcttc aatgaattca cgtcgtggtt
     6301 ctactcgatc tcccattagc atatcgaaaa tcttatctgc ttctgctgca tcatcaactg
     6361 agacacgtgc catcaagcgg ttttcaggat ccattgttgt ttcccaaagt tgatggtcat
     6421 ccatctcacc tagacctttg taacgttgta cagtaggttt tgaacgtcct gaactatagg
     6481 tatctagagc ttgacgtaat tcctcttctt gattgacccc tggctgaata taagctttaa
     6541 tctcacttcc tactttgaca ccatagattg gtggttgagc gatataaaca tatcctgctt
     6601 ccaaaacagg tcgcataaaa cgataaatca gagttaaaag taaagttctg atgtgagctc
     6661 catcaacatc ggcatcagtc ataatgacta atttttgata tctgactttt gatacatcaa
     6721 aatctgcacc aaatccagtt cccatagctg tgaagagact tcggatttct tcgtttgcta
     6781 aaattttatc cattgtagct ttttcaacgt ttagaatctt acccctaatc ggtaaaattg
     6841 cttgaaactc acgattacgt cctgattttg cagaccctcc cgcagaatct ccctccacta
     6901 taaaaagttc attcatctca gcattattag atgagcaatc agccagtttc ccaggtaagt
     6961 ttgaaatctc taagcctgac tttttacgtg ttacttcacg cgcccgtttt gctgcaatac
     7021 gggctttaga tgccaaaatt cctttttcaa caatcttctt agctacttgt ggattttcta
     7081 ataggaatcg attgaaagct tcgctaaaga gacgatttgt aattttaaca acttcagaat
     7141 ttccaagttt tgttttagtt tgtccttcaa attgtggatt tggatgttta acagatataa
     7201 ctgctgttaa accttcacga acatcttctc cagttaaatt atcttcattt tctttgagaa
     7261 tcttattctt tttagcgtaa tcattaatca cacgtgtcaa ggcagtacga aatccttgtt
     7321 cgtgcgttcc tccttcatga gtgtggatgt tatttgcaaa actcataaca gtctcttgat
     7381 atccagtagt atactgcatt gctacttcaa ctgaaatacc atctaattca ccgtcagtat
     7441 aaataggatt ttcaaaaatc acttctttgt tctcattgat aaactcaacg tatgaaccta
     7501 taccaccttc atagtgatat tctttttcaa cttcttgacc ttctcgcttg tctgaaatgg
     7561 agattcttag tccacgattt aaaaaagcta attcctgaat acgtttggct aatttatcaa
     7621 aatcaaatac agtggtttca gtgaaaattt ctgggtcagg cgtaaagtga actgttgttc
     7681 ctgataagtc agtatcaccg ataatttcaa gatcattaac aacaacaccg cgttggtatt
     7741 cttgataatg aaccttgcca tttttataaa ccttgacatc taattgtgtt gacaatgcat
     7801 taacaacaga tgaccctaca ccatgtaagc caccagaaac cttgtatccg ccaccaccaa
     7861 atttaccacc ggcatggaga actgtaaaaa ctgtctctac agctggtctt ccagtttttt
     7921 cttggatatc taccggaata ccgcgaccat catcaacaac tgtaatagag ttatctggtt
     7981 caatgtaaac tttaatatgt cctgcaaatc cagctaaggc ttcatcaatt gagttatcaa
     8041 caatttccca tacaaggtga tgaagccctt ctttcgaagt cgaaccgata tacatgcctg
     8101 gacgcatgcg aacagcttct aaaccttcta atacttgaat ctgacttgcg tcatattctt
     8161 gagcacgttg ttccatattc tttgtttctt ctgtcattta ctctattatc tccatcaatg
     8221 attttgaccc atcgactaat aaggtatcaa aaccagccga ttctcctgct tgcttatcta
     8281 tatcacgatc gcctattact aaacaattat caatttggta tttttctttc aaataaagca
     8341 tactctcggg actaggtttc cttgaaaagc cgttatcagc agtcactact tctgtaaaat
     8401 agtcaataat ttctgttttt tccagaataa ctataacttg attatcacga tgagacacca
     8461 aaaaattacg actaccttgt ctttgaattt tcgctaaaag ttcttttgcc ccttggaata
     8521 gaattggtgt ttccaaaacc ttagcttcat tagccttata ctcttttaaa aaatcagctt
     8581 cttcagggat aaaatatgca attgcatctg ctgttgactc ttttaatttt tgatacacac
     8641 tatcgtggtc tgcttgatat ccaaattctt tcaaagtctc tacaaaagca cgtgtggaac
     8701 tttcgtaatt atcaagtagt gtaccaccca aatcccaaat ataatcatga taattcatac
     8761 cctttattat atcatattat tacgattttt tatagctacc accttactta attaactcac
     8821 tcctctaaaa tatttcgatt attaaaaggc tggattatct ttccaacctt tttttgttat
     8881 atactacgat acttcaaatg tgatccatta gccttggcct gacgaactgt attttgataa
     8941 gacatggata agaccaatcc aattccaata agattactta gtaatgaact tccaccttgc
     9001 gaaataaacg gaagtgggat tcctgttaga ggtaatatac caactgctgc accaatattt
     9061 tcaaaaacat ggaaaacaat catcatgata aaaccagttg atataaaagt ataaaattga
     9121 ttattagatt caatggtgat ccttaacata cggtaaatga taatgagata taaccctaaa
     9181 acaatcgccg aaccaatgaa gccaaaattc tcagcaatga cagtaaaaat catgtcactt
     9241 tcacgaacag gaaccgagag ctctaaaata ttaaaacctt tacctgttac cccgccactt
     9301 ccaattgata ccataccctg tgtttgttga taagcaattg agtctgcaaa actaaatgga
     9361 tcaatccaag cagataaacg gttgatttga tatgtatcca tccccaaatt ataaaaccat
     9421 tccttgccgt ttggtgagat aaaaatcatc aaaaagctag ctataaaaag aacgatagta
     9481 gataaaattg gcaagattat ccaccaagat atgcccgata aaaggattaa acctgacaaa
     9541 atggcaagga aaactaatgc tgttcctaaa tctttttgta acattagaag aatcatcact
     9601 ggcaatgtaa ccaaaccaaa caatcctagc aacttccaat catcttgaaa agttttgcga
     9661 tttttctgat gaaaactaac agttattcga gataacatta aaatatagga aatcttcata
     9721 aactctgacg gttgaaacaa agttaccgag ccaatggtca cccaattttt agcacctgtt
     9781 gccgcaaaaa gctgtggact ataaaaaatt aagggaagaa ccattagggt tagtcctaaa
     9841 gcatacagat agggcgtggc tttccataaa aattcagtac tgaaaagcat aacgacaaat
     9901 gcaataatac accccataat aatccaagac acctgctgaa acatcgcgat atagatatta
     9961 gaaggataat cattcatcgt tgcaacataa attgaggcta aaccaatcat taataaaaaa
    10021 aatactggta tcacaacagc ataatcaatt tgccctttaa aatagtttga tttttgtgac
    10081 ataggacttc cttttttatt catggtaatt attataacat tttt
//
LOCUS       DATRNX010000066        10118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRNX010000066 DATRNX010000000
VERSION     DATRNX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            104..880
                     /locus_tag="VBC81_001356"
     CDS             104..880
                     /locus_tag="VBC81_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6912398.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            880..1386
                     /locus_tag="VBC81_001357"
     CDS             880..1386
                     /locus_tag="VBC81_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6912399.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            1421..1506
                     /gene="ffs"
                     /locus_tag="VBC81_001358"
     ncRNA           1421..1506
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC81_001358"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1658..3007
                     /locus_tag="VBC81_001359"
     CDS             1658..3007
                     /locus_tag="VBC81_001359"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6912400.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            3329..3856
                     /locus_tag="VBC81_001360"
     CDS             3329..3856
                     /locus_tag="VBC81_001360"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6912401.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDCEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            3847..4524
                     /locus_tag="VBC81_001361"
     CDS             3847..4524
                     /locus_tag="VBC81_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6912402.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            4656..5699
                     /locus_tag="VBC81_001362"
     CDS             4656..5699
                     /locus_tag="VBC81_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6912403.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(5790..6689)
                     /gene="galU"
                     /locus_tag="VBC81_001363"
     CDS             complement(5790..6689)
                     /gene="galU"
                     /locus_tag="VBC81_001363"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6912404.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(6726..7742)
                     /locus_tag="VBC81_001364"
     CDS             complement(6726..7742)
                     /locus_tag="VBC81_001364"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6912405.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            7912..8241
                     /gene="rnpA"
                     /locus_tag="VBC81_001365"
     CDS             7912..8241
                     /gene="rnpA"
                     /locus_tag="VBC81_001365"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6912406.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            8254..9069
                     /locus_tag="VBC81_001366"
     CDS             8254..9069
                     /locus_tag="VBC81_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6912407.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            9077..9898
                     /locus_tag="VBC81_001367"
     CDS             9077..9898
                     /locus_tag="VBC81_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6912408.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         3398 a         1566 c         1965 g         3189 t
ORIGIN      
        1 taaaaattta gatttactag tcttttttgt gcataaaatc taaaacagtt attcttaaca
       61 aaatttggta taatagcatt aaatcagtaa ggaggggatt atcatgaaat taacaacgtt
      121 aggttgttgg ggtggttatc cttataaaga tggaggaacc acaagttttc tattaactgg
      181 agaaaataat tttcacttat taatagatgc gggaagtcgt tcaatcaatg agttagaaaa
      241 agaaataagc ccgttggatt tggatgcagt tatcatcagt cattatcatc cagatcatgt
      301 agcagatttg ggtgtgttgt atcattatcg tttgctatac cctaaagata aatgggaacc
      361 caaagtattg ccagtttatg gacataatga ggatgattat gagttttcta aattgacatt
      421 acctaacgtt gctcaaggaa ttgcttatca taccaatgga gttgagcaag ttggtccctt
      481 caatattacg tttatcaaaa cagtccaccc agtcgtctgc tatgccatga ggattacaga
      541 aagagaaact ggtcaagtat tagtctttac aggagattct gggtattttg ctgaccttgt
      601 accttttaca aaaggcgcag atttattttt agcagacgtt tatctttacg agggaaatga
      661 aaatcatatt gcacatttga ctagtaaaga agcaggtgag attgcttata acgctaaagt
      721 taagaaatta gttttaacac atgttccgca gtttccggga gaaggaataa accccgaaaa
      781 tcatttggaa gaacttcgac tacagtctga aaagtatgca ggtggcatct ctgttgattt
      841 agctcagcca cataaatctt gggacttagg aaaactataa tgcagaaatt tacccaagaa
      901 gaaaaagaat ggtttatgac agaagctcta aaggaggcaa aatcctcctt agagaaagaa
      961 gaaattccta taggttgtgt cattgtaaaa gatggtcata ttattggaag aggacataat
     1021 gctcgtgaag agtttaataa agctatttta catgctgaaa tcatggctat taacaatgca
     1081 aatgagaagg tagggaattg gcgcttatta gatactactt tatttgttac tgtagaacct
     1141 tgtgtaatgt gtagtggagc tattggctta gctagaattc cccacgttat atatggcgct
     1201 aagaatacga aatttggagc agcgggaagt ctctatgata ttttaagtga tagccgactt
     1261 aatcatagag ttgaagttga aaccggcatt ttagaagatc aatgcgctaa aataatgcag
     1321 gatttctttc gaaaaggacg acaacgcaag aaagaagcta aactaagtga aaaaactaag
     1381 atttgatagc ttgcaataaa atattagttt tgttataata tctttggagc aacatttact
     1441 cgtgaagtgg gtcaggggag gaatccagca gccctaagcg atgctaaatg tgtgctcttt
     1501 ttattttcaa aagtcaaagt gtttttggaa ataattatga aagtttcata aaaattggta
     1561 gcgtttcaaa aaatatgaaa atacagttaa aaaaccttga aaaaaaactc acaaaagagt
     1621 aagatgatag tgaaacataa ttgttaggag aaataaaatg acacatatta catttgacta
     1681 ttcaaaagtc ttgggtcaat ttgtaggcga acacgagtta gactacctac aaccacaagt
     1741 aagtgcagca gatgctttcc ttcgtcaagg gactggtcct ggatcagatt ttctcggatg
     1801 gatggacctc ccagaaaact atgacaaaga agaattttct cgcattcaaa aagccgctga
     1861 aaagattaaa tcagatagcg aagtactcgt ggttattggt attggtggtt cgtaccttgg
     1921 tgcaaaagca gcaattgact ttttgaataa tcattttgct aatttgcaaa ccgcagaaga
     1981 acgtaaagcg cctcagattc tttatgctgg aaattctatt tcatctactt accttgccga
     2041 tttagttgaa tacgtccaag ataaagaatt ctcagtaaat gtcatttcaa aatcaggtac
     2101 aacaactgaa ccagcgattg ctttccgtgt atttaaagaa cttctagtta aaaagtacgg
     2161 tcaagaagaa gctaataaac gtatctacgc tacaactgat aaagttaaag gtgccgtaaa
     2221 agttgaagca gatgctaaca attgggaaac atttgtagtt cctgataatg ttggtggtcg
     2281 tttctcagta ctgacagctg taggtcttct tccaattgct gcttcaggag cagatattac
     2341 tgctcttatg gaaggcgcaa atgctgctcg taaagattta tcttcagata aaatctctga
     2401 aaacattgct tatcagtacg ctgcagttcg taatgttctt tatcgcaaag gttatatcac
     2461 agagatttta gctaactacg aaccatcatt acaatacttt ggtgaatggt ggaaacaatt
     2521 agcaggtgaa tcagaaggta aagatcaaaa aggtatctat cctacatcag caaacttctc
     2581 aactgatctt cactcacttg gtcaatttat tcaagaaggt taccgtaacc tctttgagac
     2641 agttgttcgc gttgagaaac ctcgtaaaaa tgtgactatt cctgaattaa ccgaagattt
     2701 agatggtctt ggttatcttc aaggaaaaga tgtagatttt gttaataaaa aagcaacaga
     2761 tggtgtgctt cttgctcata cagatggtgg ggttccaaat atgtttgtaa cgcttcctac
     2821 acaagacgct tacactcttg gttacactat ttacttcttt gagttagcaa ttggcctttc
     2881 aggttatctt aactcagtaa atccatttga tcaaccgggg gtagaagcat ataaacgtaa
     2941 tatgtttgct cttctaggta aacctggatt cgaagagctt agcgctgaat tgaatgcacg
     3001 tctttaaaaa tatctaacta aaaagttcct caaagagagg aactttttag ttttgttgta
     3061 ggataactaa aaacccacta gcactggtag cgatagtggg ttggaaaaag ggttgagaaa
     3121 agttattaac ttttctcagg agatttatga aaaaggaaaa taatttagga taatattttc
     3181 ctaacggcta ggataaaccg cttattttag gatgatgcta gtatacagta gaaaccttaa
     3241 ataaacctta aaaattcata ctttatcatt ttattttccc ctatactatt tttgtataaa
     3301 tggtcaatat taatggtatc atagatatat ggaaaagaaa ttattaagaa aagaagtttt
     3361 gataacctta aaatctcaac ctcaagctta caaatcagaa gttgactcta aacttttaga
     3421 ggcttttata aaaactaaag cttatcaaaa tagttgtgta attgctactt acctatcttt
     3481 tgactacgaa tacaatacac aacttcttat taaacaagct ttatgtgatg gcaaacgtgt
     3541 ccttgttccc aaaacttatc caaaaggtaa aatgattttt gtggactatc aaaaagataa
     3601 cttgagggca acaccgtttg gcttattaga acctgttaat gatagagctg ttgaaaaagc
     3661 tagtatagac ttaattcatg tgcccggact tatctttaac aataaaggtt ttcgtatagg
     3721 ttatggtgca ggttattttg atcgctattt gagtgattgt gaaggtgata ctattagtac
     3781 tatttatagg tgtcagagac aggattttgt agaagagaag catgatgtag cggttaagga
     3841 ggtattatgt ctatgaagaa gttcgctaaa gagtatccga cgacagtact cttggtcggt
     3901 ctgacaaccc ttgttttttt attgatgcaa ctaacttatg gaagtcaagc agagtctagc
     3961 caagttattt ttcaatttgg tggaatacaa ggtgattatc taaaagcata tccaacaaat
     4021 ctatggagac taataagtcc tatctttgtt catattggtt gggaacattt tttattaaat
     4081 ggtttagctc tgtattttgt gggacaaatg ggggaaagta tttggggaag tttgagattt
     4141 ctaattctct atattttatc tggcttaatg ggtaatattt ttacgctatt ttttactcca
     4201 cacgtggtgg cagcaggagc ctcaacgtct ttatttggtg ttttttctgc tatagcaatc
     4261 gcaggttatt ttggaaaaaa tccttattta aaacaagttg gaaaatcata tcaagtgatg
     4321 atcttgttaa atttattttt taatatcttc acacccggag tgagtttagc aggccatgtt
     4381 ggtggtttag ttggtggagt attggtggct atttttttga caaaacaaaa tggttcttta
     4441 ttatttaaaa cttggcaaag tattttagct ttaatgatat ttattattgt atcgatcagt
     4501 ttaataggtc tatcgttggt ataattctac ggctatagtc tctaaaacca aactttcttt
     4561 gggaaaaata gaataaagtt tgctaaatga tttttttatg ttatgataga aatctgtttt
     4621 tatgtgacta taacttatta ggaggttcat tctcggtgaa cagaaaaatt attggcttaa
     4681 ctctactatc tttatctgta ctaacattaa cagcatgtgg taatcgttca gataagtctg
     4741 caaataaatc agatatcaaa gttgcgatgg taactaatca aggtggagta gacgataaat
     4801 cgtttaatca atcagcttgg gaagggctac aaaagtgggg aaaaaagaag gggttaacaa
     4861 aaggtaatgg ctttgattat ttccagtcat ctaatgaatc agatcatgct aataaccttg
     4921 atacggcagc ctctagtggc tataatttaa tttttggtat tggatttggt attcatgata
     4981 ctattgaaaa agtatcagaa aataataaag atgttaaata cgttattgta gatgatatca
     5041 ttaaaggtaa agaaaatgta gctagtgtta cttttgcaga caatgaagct gcttacttag
     5101 ctggagttgc tgcagctaag acaactaaga caaaaacagt tggttttatc ggagggatgg
     5161 aaggcgtcgt tgttaagcga tttgaagcag gatttaaggc tggagtaaaa tcaattgatc
     5221 cggctattaa agttgcggta tcttatgcag gctcatttac agatgcagct aaaggtaaaa
     5281 cgattgctgc cacacagtat gcaacaggtg ttgatgttat ttaccaggct gctggtggta
     5341 caggagcagg aatttttagt gaggctaaaa ctgaaaatga gacccgcaag gaatctaaca
     5401 aagtctgggt aattggtgtt gacagagatc aaagtcaaga aggcaattat gtttcaaaag
     5461 atggtaagaa agcaaacttt gttctggcat ctactataaa agaggtgggg aaatctctcc
     5521 aatctgtagc agaattaaca gagaaaaaac aatatccagg agggaaagta acagtttttg
     5581 gtctaaagga tagtggtgta gatattaaag agcatcaatt gtctagtgag ggatctgttg
     5641 ctgtcaaaaa agctaaagag gggattgttt cagggaaaat acaagttcct atgaaatgat
     5701 aaaagtgtgg aggaacaata agttcttcgt caagtacaat aaaaaagttt cctaaatagg
     5761 aaactttttt attgtacttg actcatattt tatttcttag atgttttttc tagagatttt
     5821 cctaaatcaa tgatatattt cttcaagtca tctttaactt gtgggtgttg aagagcataa
     5881 tcaatagaag ttttcataaa accaaacttg tcaccaacgt cataacggtc ccctgtaaat
     5941 tttctagcaa aaacacgttg cgttttattt aaagtatcga tagcatcggt tagttgaatc
     6001 tcatttcctg caccaggttt ttgagtttct aaaatattaa aaatttctgg agttaacaag
     6061 tagcgaccaa tgattgccaa attacttgga gcctcttcgg gagaaggttt ttcaacgaaa
     6121 gtgttaacgc tatatagacc attaactcct tctccttgtg gagcaataac accataagca
     6181 gaaacatctt catgtggaac ttccataaca gcaattgttg atgcatgagt agcttcaaag
     6241 tcattaatta attgtttggt gagaggaata actttattat tggtaatatc cattagatca
     6301 tcacctagca taacaacaaa aggttcatta ccaacgaagg cttttgcttg tagcacagca
     6361 tctccaagcc cacgaggatg actttgtcga ataaaatgga gtcgaatgcc agttgtttca
     6421 tcaacaagtt tgagaagttc gtttttccct ttctctttta ggttatattc taattcaaaa
     6481 tttgaatcaa agtggtcttc tatagaacgt tttgatttac cagttacaac taaaatatct
     6541 tcaattccag attttagtgc ttcttcaaca ataaattgaa tagttggttt atcaacgatt
     6601 ggaagcattt ctttagctaa agctttagta gcaggtaaaa aacgtgttcc taaaccagcg
     6661 gcagggataa ctgcttttct gactttcata tatagctcct ttaaatagta ttaatataaa
     6721 cgatattagt gccattcatt ctctgaacga aattcattgg acatcatatc taaaatgcta
     6781 tctttgatat tagccccttc atatattgat ttataaatag cttctgtaat gggcatgtaa
     6841 acatttaagt tttgggcgat ttcgtaagca accttagttg ttgagatacc ttcaataacc
     6901 atccccatat ttttttcaat atcttcaagt ttctcaccac gtcctaaagc atctcccgct
     6961 ctccagttgc gagaatgaac agatgtacca gtcacaatta aatctccaac tccggataaa
     7021 ccactgaaag tgagagggtc ggcccctaat tgtacaccaa ggcgtgttat ctcagctaaa
     7081 ccacgcgtaa taatagctgc tttagcatta tcaccataac caagaccgtg tagtgctcca
     7141 gcaccaacag caataatatt ttttagagca ccagctgttt ctactcctac gacatcagtg
     7201 ttagtataaa gtctaaaata atgattacta aacaattttt ggacatactt agctgcttca
     7261 atatcttttg aagcagcagt aatcaaagta atatcacgaa cgatggcttc ttcagcatga
     7321 ctgggacctg aaacaacaac gatatcacta cgatattgtt cagagatttc ttcttctaaa
     7381 attgtagaaa gtcgttcatg agtgccaggt tctagcccct tagaagcatg cataattaca
     7441 actttatgct ttaaaagact agccacttgt ttagcaacaa gtcttgtaac tttagttggg
     7501 acgacgaata gaatgctgtc aacattatta atcgcttcct caagattagt gtaggctttt
     7561 attttggaat ctaaggtaat atctttgaag taacgttgat tagtatggtt agtattaatc
     7621 tcttctattt gttcaaccac gtttccccat aagcgtacct catgtccatt gtcatttaat
     7681 acttgtgcaa gagcagtccc ccatgaacca ggtccaagaa cggcaatttt ttgttttgtc
     7741 atcttattcc ttctttctaa taataaggca tatagcgcta acattatatc atactctaat
     7801 acactttgtc ttgtttagtc tgttgtcgat gatagtagca ttagaaaaga attgagtctt
     7861 ttaggttatt tatggtataa taaaaccaat ttagataaat ggagcactat tttgaaaaaa
     7921 acctatcggg taaaaagtga taaggatttt caaatgattt tcagcagagg taaaaatgtt
     7981 gctaatcgaa aatttgttat ctactattta gaaaaagaac aaaaacactt tcgtgtggga
     8041 atctctgtta gtaagaaatt aggaaatgct gttgttcgta acgctgttaa aagaaaaatt
     8101 aggcatgttt tactctctca gaaaacagcg ctgcaagatt atgattttgt cgtgattgcg
     8161 cgtaaaggtg tagaggaatt agactatcaa gcattagaaa aaaatcttat acacgtttta
     8221 aaaatagcag gactcatcta gaaaggtata aaattgaaga aaaaacttaa aacatttagc
     8281 ttaatattgc tgacaggttc tttattggtt gcttgcggac gaggagaagt atctagtcat
     8341 tcagctacat tatgggagca aatagtttat gcatttgcta aatcaataca atggctctcc
     8401 tttaaccatt caattggttt gggaataatc ttattcactc taataatcag agcgattatg
     8461 atgccgcttt ataatatgca aatgaaatcc agccaaaaga tgcaggaaat tcagccaagg
     8521 ctcaaggaat tacaaaaaaa atatccaggg aaagatcctg atagccgttt aaagctaaat
     8581 gatgaaatgc aatctatgta taaagctgag ggagttaacc catatgcaag tgtattacct
     8641 cttctaattc agttacctgt cctatgggct cttttccaag ctttgactag ggtttctttc
     8701 ttaaaagtag gaacgttttt gagcctagaa ttatcacagc cggacccata ttatatttta
     8761 cctgtactgg cggccttatt taccttttta tcaacttggt taactaacaa agcagctgtt
     8821 gaaaaaaata ttgctttaac tcttatgact tatgttatgc cgtttatcat attggtaaca
     8881 agttttaact ttgctagtgg tgttgtttta tactggacgg tgtctaatgc ttttcaagta
     8941 tttcaaattc tattattgaa caacccatac aagattataa aggttcgaga agaagcagta
     9001 agagtggcac acgaaaaaga acaaagagtt aaacgtgcta agagaaaagc aagtaagaaa
     9061 agaaagtagg aaaaacatgg tattatttac aggagcgact gtagaagaag ctattgaaaa
     9121 aggactacaa gagttaaaca tttcacgatt gagggcacat ataaaagttg tatcacgtga
     9181 gaaaaaaggt tttttgggct ttggtaaaaa gccagcaaaa gttgaaatag aaggtatcac
     9241 cgacgaagta actgatatta atgaatcggt agcgttaaaa aatattaaga atgtaccgtc
     9301 tagcgtagat gtggtggaag aatatataga ggaggttgat gaaacgcttg aaaaagaaga
     9361 cgtatcacaa ccagaacttc caaaaattga tgataaaaac gttgtaacta caagtgaagc
     9421 tattgaaaaa attgacctac ttcctaatat tgaagtagca gctgctcagg tgactaaata
     9481 cgttgaaaat atcatctatg agatggattt agatgcaaca atagaaacta cgacaagtaa
     9541 gcgacaaata aatctccaaa tagaaacgcc agaagctggg cgcatcattg gttatcatgg
     9601 gaaagtatta aaatcgttgc agttgttagc acaaaattat cttcatgacc gcttttctaa
     9661 gagtttttca gtatctatta atgtccatga ctacgttgag catcgtatag aaactttaat
     9721 tgatttttct aaaaaaatag ctagacgtgt tttagagact aatgagcctt accatatgga
     9781 tcctatgagt aacagtgagc gaaaaactgt tcataaaaca attgcaacta tagagggtgt
     9841 tgaaagttat tctgaaggta acgatcctaa tagatttgta gtagttacta agaagtaaaa
     9901 agaggtttca cctctttttt tcttgcgccc gatctacatt ttcagaaaat gtagaaaaag
     9961 tatgaaaaaa gagttgacaa agtaagagat gatgtagtaa actaaataag ttgtctcgag
    10021 agagcagttt ctagtagcgc gtagttaaca acttaaaaaa agtggttgac aaagcagaat
    10081 aagtttgcta gaatatagaa gttgtctcaa gagaggca
//
LOCUS       DATRNX010000067         9738 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRNX010000067 DATRNX010000000
VERSION     DATRNX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBC81_001368"
     CDS             complement(<1..782)
                     /locus_tag="VBC81_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6912409.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBC81_001369"
     CDS             complement(782..2125)
                     /locus_tag="VBC81_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6912410.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBC81_001370"
     CDS             2265..3116
                     /locus_tag="VBC81_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6912411.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBC81_001371"
     CDS             3119..4078
                     /locus_tag="VBC81_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6912412.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTQKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBC81_001372"
     CDS             4132..5484
                     /locus_tag="VBC81_001372"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6912413.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBC81_001373"
     CDS             5607..5978
                     /locus_tag="VBC81_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6912414.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBC81_001374"
     CDS             6003..6383
                     /locus_tag="VBC81_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912415.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBC81_001375"
     CDS             6476..7606
                     /locus_tag="VBC81_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6912416.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="VBC81_001376"
     CDS             7610..8347
                     /locus_tag="VBC81_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6912417.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="VBC81_001377"
     CDS             8348..9118
                     /locus_tag="VBC81_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6912418.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..>9738
                     /locus_tag="VBC81_001378"
     CDS             9108..>9738
                     /locus_tag="VBC81_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6912419.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRK"
BASE COUNT         3189 a         1503 c         1906 g         3140 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcagaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cgatttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct acaatttata
     9181 ttgcttggtt gatctatcct atagaaattc agtttttaaa attagaaaag gttgtgtatt
     9241 taaaggctga aacaatttat tataatttta ataagttgat gatatatctg actcatcctt
     9301 ttatctctga tttaaatatg cctagctttc cttcttcaga ggatggttta aagcattttg
     9361 cggatgtaaa atatcttttt actctagcgc atgggctttt tgttatacta acttttccag
     9421 ttatttattt tttaagacgt ggatggaagc aaaaaagtat ctttctatat gaaggcttct
     9481 tcaagatagc tattatgttg cctattttta ttgtagtatg tgcttttctg ttgggttttg
     9541 atcagttttt cactctattt catgaggtgc tttttccagg agattctact tggcaattta
     9601 atcctctaac ggatccagta atttggatac ttcctgaaac ttttttcctc cattgtttca
     9661 tcattttttt gttaatctat gaaacaataa ctataattct tttgataata ggtagaaaac
     9721 atttaaaatt aagaaaat
//
LOCUS       DATRNX010000068         9724 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRNX010000068 DATRNX010000000
VERSION     DATRNX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1832)
                     /locus_tag="VBC81_001379"
     CDS             complement(<1..1832)
                     /locus_tag="VBC81_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6912420.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLM"
     gene            complement(1912..2727)
                     /locus_tag="VBC81_001380"
     CDS             complement(1912..2727)
                     /locus_tag="VBC81_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6912421.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(2714..3580)
                     /locus_tag="VBC81_001381"
     CDS             complement(2714..3580)
                     /locus_tag="VBC81_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6912422.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKTALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(3616..4110)
                     /locus_tag="VBC81_001382"
     CDS             complement(3616..4110)
                     /locus_tag="VBC81_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6912423.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(4165..5361)
                     /locus_tag="VBC81_001383"
     CDS             complement(4165..5361)
                     /locus_tag="VBC81_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6912424.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(5364..5798)
                     /locus_tag="VBC81_001384"
     CDS             complement(5364..5798)
                     /locus_tag="VBC81_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6912425.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            6076..6888
                     /gene="dhuD"
                     /locus_tag="VBC81_001385"
     CDS             6076..6888
                     /gene="dhuD"
                     /locus_tag="VBC81_001385"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6912426.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            6905..7543
                     /gene="dhuI"
                     /locus_tag="VBC81_001386"
     CDS             6905..7543
                     /gene="dhuI"
                     /locus_tag="VBC81_001386"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6912427.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            7569..8576
                     /locus_tag="VBC81_001387"
     CDS             7569..8576
                     /locus_tag="VBC81_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6912428.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            8588..9226
                     /locus_tag="VBC81_001388"
     CDS             8588..9226
                     /locus_tag="VBC81_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6912429.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         3123 a         1886 c         1627 g         3088 t
ORIGIN      
        1 tgcatcaaat agagtttatc cccaaagata atatcatcca tatgcactcc aattaaataa
       61 tgaaagtctt gagacctaat atcaaatgca atgctattac ttagttcctt atgactgttc
      121 gtttgcacta aaggaacaca tttcacctga cagttccgat caactatcaa ggtataatcc
      181 atcacctcat caacaaattt tattttctta actaatttat gactttctgt caattgatta
      241 taccgcttag aaataaggca atcttctaga ttaaaggtcg taggacttac taatttcaag
      301 tcgttaacaa gcccattttc ataaatgacc tgatcatcta aaatatattg atttgttaaa
      361 acatgctctc ctggacaatt caccttatca ataactaagg taatggcctg cggtaaagtt
      421 aacaaccatc gcttatggta atagatatca gaagctcttt ggacatgtag ttgtccttcg
      481 actaaatggt acctatcctt tgaagtcagc tgacaatagt cacattttgg ataagaattg
      541 tatgtccaag agtccttgat catttctaaa gattgcccat caagggtaca tgttgaatga
      601 ctagtcgaac gtttaaaatc ataccttagt tgttcttctt tgtaagtata tcttcctgca
      661 tcaatgaaaa taggtttctt tttatcatag agacaaacac tattattatc actatgagta
      721 tgagcgctac caaatggacc gttcttaaaa aatatataac gcctatcatc acgtaaacac
      781 acatgaccag aatctggaaa aagataagca gactcaccta ttgttgcacg cggtatttct
      841 tcaaaaagat aaattgatgg cttcccaaac aaaagtaaag tttctaaatt aacattatca
      901 aaagaaaatg attttgtttt agaagatttc aacacaagtg ttgccaaagt tagaatatca
      961 cgagtatcgg tcacatcact gtcccctata gctagttgac aataatccgg accagtcata
     1021 gcaattagat aggtaaccat tttttcaata gtttcttcta atggtagata atatttaggc
     1081 gcaaggataa ctagttccat taaggacttc aagacttcta catgatacat aattgattgt
     1141 tcatactggc tcccgtcatc taaaatttga agcttgattt gaagtaataa ttcttcttca
     1201 gcaaaactct gaatttcagg taggtttaat tcatcttcat aatagtataa acacgccaat
     1261 attgctgttg tttgtaaaat tccccagtta cttagactat ctttttcacg ataatagtcc
     1321 ctcatataag ttatctgctc ccgtaaacta gttaatagtt taattttttt agtttctgtt
     1381 attaatccaa aataatctaa aaaaatcaaa actttgagcc atgacatgca acgtatccca
     1441 gtatcaatag tacgacttac cgccccttct ggctttagtg taaattggca atcaatccaa
     1501 tggtacataa aatatttcat ttgtctaaga taagacttat cgccttcaac aatgtagacc
     1561 accaggaatt taaaaagata agtttgacga tttagcatat acaaccattc tggatcatct
     1621 gttactggat tatcccactc caaaggatca agacaatatg ctttagaaca aggttccata
     1681 tcccaattat cattaaatac aaaagtgttc ctcatcatca aattaacaga ggctttcatt
     1741 tcttgatagg cattagattg gtatgtttta atatactttt gacaaaattc cctatcaaaa
     1801 tccttgaatt tattatataa gcgatctttc atttttttac aaaacgtcct cgtccgatat
     1861 aaaaaggact atgtcattga tatatcataa tccttattta tcaaaatttt attaagctaa
     1921 aatacctaac cagctaccaa ggataccaat aataaccgta aggataacta gcttataagt
     1981 tgtccatttt ttatttttaa taaggtaaaa cataaggaaa gtgtagagtg ctggaagtag
     2041 agctggtgca attttatcaa gcataccttg gacagtaaca attttttgcg ctgtactagt
     2101 tactttttct ggagcaaaag tgtaagggat tgtaagctta acttgtgttg cagctaaact
     2161 tgagatgacc gttacaccaa cgatattagc tgcccgtgag ataactgaca tttgttcact
     2221 caatttatca ataaaactgg tcccaagacg ataaccatac atgccagtaa ctaatttaat
     2281 tcccgttaaa atagtattca tagccacaaa gaacagaatc ggtcccatca ccaaaccatc
     2341 tgtcgctaaa gaggctgcaa tcgttgagaa caagggtgct aagcaaaatt gtgataaaga
     2401 atcaccgata cctgcaagag gacccataag cgccatctta ataccgcgaa tttcttcttc
     2461 tggccgctca ttatcaagca ttgctaaatg taaactagtt acaaaaggta agaaatgaag
     2521 gtttgtattg taaaactcca cattttcttc tagagctccc gctaaagctt ttttgtcatc
     2581 tccataatat tttttgagag ctggatatat gacattagca taaccaagac cttgatagtt
     2641 actgtaatta aagccatttt gtaaataaaa ggcacgtaaa gctgttttag tgtaatcact
     2701 ttttgctaat tttttagatc cagtcatctt ggttgtctcc ttctgtctta gtttctttag
     2761 taggtgcagc tggttttctg aaaatatcga gcaaagcaaa tattgttgca attatagcta
     2821 ccccgatagt tggaacccca agcacactgt tagtagccac tgatgttaaa actccgtctc
     2881 catttgctgt tggagttaga actggcaaac caaaataaac agctagaaca tatccaagta
     2941 ggatataagg aatgagttct tttttagcca ttacagagag aatcatcgca aaaccaattg
     3001 caggaagcat tttcccagct aaggttagac cattaagcaa aacaggggga atgagagcaa
     3061 ataaatctgt caaagtttgc gttgaaagag cacccagtac tcctaaacca aatcccaatc
     3121 cagcgaatgc ccaaattgta ccatttgctg caattttaaa tcctcggaaa ttacctgctt
     3181 gcaaagctgt tttagctgtt tcaggcgcac ctgcaaaagc agtataagtt gctgtttgaa
     3241 gaaattgaat accaactgca attggggttg ataatgcaag agcagcttcc ggtgtgattt
     3301 ttcctttagt tccagctgtt gtaatagcca tcaaagttcc aaaaatccca ggaccgattg
     3361 ggtttggagg aactgtccct cccgctccca caccaaatcc catgtaagcc agttcagata
     3421 ttgctccaac tgccaaagct gtttgaatat ctcctaaaat aacaccaacg cctagagaga
     3481 gaacgataca acgatttgta tagaggccaa gcaacatgcc actaaaacag aaagccgtcc
     3541 acagtccaat taagacagcc tgaagaatac taatatccat atttttctcc tttatatgat
     3601 agtgagtttg tccaactaaa tataatctaa aatattaact tccacagcac catcgttacc
     3661 tgttggagtt gttttagtat taaatgcaat gtgatattct tggtttaatt ttctaattat
     3721 ctctttatcc ttcatcccta agaagataga gcgacttacc tgctctttac cttctccatt
     3781 atgaatattt ccaatattaa tctctttaat tgggacgcct cctgctacaa gtctatagac
     3841 atctttcaaa tctttaacaa ttataaaaat cgtctgtgct gggttggcct tgtgaataat
     3901 atcaataact ttttgaatat caaaaaatcg caatgcaatt gattctggca ccaccgtctt
     3961 cattaacgtt tgttgaagat gatctttgga gacatcatca tttgccacaa taacagtatt
     4021 acaacttaaa aatttcaccc aaagttgtcc ttgtccatga ataagacgct catcaacacg
     4081 tgtcattaca atatttggtc ctgctgccat ttctgacctc cttgttttct attatttatt
     4141 gtttattttg ttagtaaaat actcttacca ataaagttcc cagtctttat agaatcttat
     4201 taaggcttct aagtaataat aatctcccca aatattacct tcatctactc ctttacctga
     4261 atgccacgaa tacacaccgt gcaatagaag aggacgtcct gctataaatt cactattact
     4321 atactgttca atcagactac gaagcattgt atgcatagca tatttgtatg tctctttatc
     4381 aggatctact tctggtaaat gtttaagcat ctcatgaatc ccacacacag ctgttgctgt
     4441 tgcggatgta tctctaggct gacccgagcc atccgtaaaa ataaggtccc aataggatac
     4501 cttgtcttca ggtaaacgat ttagaaaata gtttgtcata cctttaaaaa ggataatctg
     4561 ctgataatct ttcattttcc ggtaactaag cggaataccg tagatgcccc atgcttgccc
     4621 tcttgcccaa gatgactcat cactataacc ctgtcgtgtg acacctttta acggttctcc
     4681 agtttctggg tcgaaataaa aagtatgaaa agcagaagaa tcatcacgca ccacattgtt
     4741 agctgaagcg tagaagtgat tcaccgcaac ttgtctatac ttttcatcac ctgtctgttc
     4801 ataagcaaaa aataagagtt ggatattaag taagcaatcg ataattaagc gatagtgttc
     4861 cttatacccg agttctcccc aagcctgaat aaatccacct ttttcttgat agcgctccat
     4921 caatttatct gcagctttta tagtggcttc taaagcttta gcatcaccat tgatacgata
     4981 ttctgctgta caagatggtg tgtaaagaaa tcctaagtcg tggtgatcta atgctatacg
     5041 attattaata cgatttagaa atgacaatac atttttgtgg gctatgtttt ttaacttttt
     5101 atcctgatta tactcataag ctaaccacaa gcaccctgtc caaaaaccat ttgtccattc
     5161 tgtgttatcc ataactttat aagtattatt gaatgttgca ggcgttggat actcttcacc
     5221 aaagtaatca atattaatat aaagttgctt caaggctttt tcaatggctt cctcaacttc
     5281 aatcttagtt aataatctac tgtctaaaaa acgctttgga ttttcaattg actcaacctt
     5341 gaccggtttt attttcatca ttattaaatg cctccctcaa aagttgcttc tgcagtttgt
     5401 gtagcaaggc aagtagaaaa atcaacaatt ccttcatgag atgatgttat aactttatca
     5461 acaaggtcat ctacatgttc aaacatcgtt ctagataaaa cagcttccat taacattgaa
     5521 aggttcaagc ccgataaaac ttttatcttt ttatctgtat attctactga taaggcggaa
     5581 gaaacattga aaggagtccc acctagcaaa tcagtcaata ctaaaatttc tttgaaatcg
     5641 ataacctctc tttgcaaagc aactctaaca tcattagaag acatgcctgc aataaagtta
     5701 ataccaacaa catattcctg atgcccagca ataagttcta aagagcttaa aataccatct
     5761 ggaaagttgc cgtgtgctac aataataatt tttatcatat tttctcctct tgagtttacc
     5821 ggtttacttt tataataaga ttatagcgct ttcattttta ggtgtcaaca tcttttttat
     5881 attttttgag cagtttactt gattttttat aatgatgtct tttttatctt taagaatcat
     5941 aacttagata aagctttgtt atatatgttg ccaagtttta taggagaaga actctatagt
     6001 tgacaaaatc ggtttacttt gttactattc atgtaaacag gtttacttcc tgaatttttt
     6061 tacggaggtc atattatgac tgaacaattc ttaaaagaca acttttcact agaaggtaaa
     6121 gtcgccttaa taacaggcgc ttcttacggc attggttttt caatagcaac tgcttttgct
     6181 agagctggag caactattgt cttcaatgat attaagcagg aactcgttga caaaggcatc
     6241 tccgcctata aaaaacttgg cattaaggcc catggctatg tttgcgatgt tacagatgaa
     6301 gatggaatca acgaaatggt gaacaaaatt tctcaagatg ttggggtaat tgatattcta
     6361 gttaacaacg caggtattat aaaacgcacc cctatgctag agatgagcgc tgcagatttt
     6421 cgacaagtta ttgatattga tttgaatgct ccctttatcg tatcaaaagc ggtattacct
     6481 ggaatgatcc aaaaaggaca tggtaaaatc atcaatattt gctccatgat gagtgagctt
     6541 ggacgcgaaa cagttgctgc ctatgctgct gccaaagggg gacttaaaat gttaaccaag
     6601 aacattgctt ctgaatacgg ttctgctaac atccaatgta acggtatcgg gcccggctat
     6661 attgccacac cacaaacagc tccccttcgt gaacgtcaag atgatggttc acgtcatcca
     6721 ttcgaccaat ttatcatcgc taaaacacct gccgctcgct ggggtgaggc agaagattta
     6781 ggtgcaccag ctatcttcct tgctagcgat gcatcaaact ttatcaatgg tcacatactc
     6841 tatgtagatg gcggtatttt agcttacatc ggtaaacaac ctcaataata aaggagtaat
     6901 ataaatgaaa attgcattaa tcaacgaaaa tagccaagcg agtaaaaata ctcttatcta
     6961 caaagaattg aaagcggttt ccaaaaaaaa aggatttgat gttttcaact acgggatgta
     7021 tggcaaagaa gaagaaagcc agctaactta tgtacaaaat ggtttactga ctgctatttt
     7081 acttaatagc ggtgctgcag attttgttat tacaggctgt gggacaggaa ttggagcgat
     7141 gcttgcttgt aatagctttc ctggcgtagt ctgtggattc gctgctgatt ctgtcgacgc
     7201 ttacttattc tctcaagtaa acggaggaaa tgctctttcc cttccttttg ctaaaggatt
     7261 tggttgggga gctgaattga atctgaggta tttatttgaa cgtcttttcg aagatgaaaa
     7321 aggaggggga taccctaaag agagggctgt acctgagcaa cgcaatgcac gtatccttag
     7381 cgaagtaaaa cagattactt acagagattt actctctgtt ttaaaagaaa ttgatcaaga
     7441 tttcttaaag gaaactattt ctggggagca tttccaagaa tatttcttcg ctaattgcca
     7501 aaatcaaaat atagcagact acttgaaatc tgtcttagac taaagcgtct gttttagaaa
     7561 ggacttttat gtctaagatt ctttttttcg gtgaacctct cattcgcatc actcccaaag
     7621 aaaatgacta ttttgcagat agtatctcaa ctaaactatt ctatggtggc tctgaagtta
     7681 atatcgcaag agctctacaa ggctttgggc aagatactaa actcctctca gccttaccca
     7741 ataatcccat aggtaatagt tttcttcaat ttttaaaagc acagggtatt gatactcatt
     7801 ctattcagtg ggttggagaa cgtgttgggt tatatttctt agaaaattcc tttgcttgca
     7861 gaaaaggtga ggtggtctac gaccgcgatt atagtagttt acatgacttt aggataaacc
     7921 aaattgattt cgatcaatta tttgaaggag tttctctctt tcattttagt ggtattacac
     7981 tatcattgga tgagtctatc caagagataa cactgcttct tttaaaagaa gctaaaaaaa
     8041 gagagataac aatctcctta gatctcaatt ttcgcagtaa actaatttct cctaaaaatg
     8101 ccaaaatcct tttttctcag ttcgctacct ttgcggatat ttgttttggt attgaacctc
     8161 ttatggttga ctcacaggac actaccttct tcaatcgcga tgaagctaca atagaggaca
     8221 tcaaagaacg aatgatttct cttataaatc actttgactt tcaagtcatt ttccatacta
     8281 aacgtctaca ggatgaatgg ggacgaaacc actaccaagc ttacatagca aatcgtaaac
     8341 aagagtttgt cacttcaaaa gaaatcacca cagctgtaaa ccagagaatt ggaagcggag
     8401 atgcttttgt ggcgggagcg ctatatcaac tacttcagca ctcagacagc aagacagtca
     8461 ttgatttcgc tgtcgctagt gcatctttaa aatgtgcttt ggaaggggac aatatgtttg
     8521 agaccgttac cgcagttaac aaagtactaa atcaatcgaa agacattatt cgttagaagg
     8581 gagaattatg ataaaatcaa agattttgtt gcaattaatg caaggaaaaa tagttgctgt
     8641 cattcgtgga aatagccaag aagaagcttt tcaagctgct caagcatgca taaaaggtgg
     8701 tatttctgct atcgaaatcg cctatactaa tagcaaagct agccaagtca ttgaacaact
     8761 agtcacacaa tatactaacc aagaacaagt tgtcgttggg gcaggaactg ttctagacgc
     8821 agaaactgcc cgaatggcta ttttagcagg agcaaagttt attgtatctc ccgcatttaa
     8881 tctacagact gctaaattat gtaatcgtta tgctattcct tatttaccag gttgcatgac
     8941 cttatcagag ataacaacag cacttgaagc tggttgtgaa ataattaaaa tattcccagg
     9001 aggtactcta ggaacatctt tcatctcatc actaaaagca cctttacctc aagtacaaat
     9061 tatggtgact ggtggggtaa atctcacaaa tgccaaagac tggtttctat ctggagtaac
     9121 agccattggt attggtggag agttcaataa actagcagct ttgggggaat ttgataaaat
     9181 cacagaaatg gctaaacaat acaaaataca gctaaatctg aattaacaca ataaaaagac
     9241 aaggcacccc cacacctcaa tcatgagaca gaagaaaaga acttttataa agccattttt
     9301 ctgtagtacc gtttatctta atcagactca ttatctcgat tttcttatca gaccgagata
     9361 atttttagct aatcttcctg tagacataac cgttctactt ccgacaactg atccatgtct
     9421 tcaccaaaga tatactactt gcccgcagat tgatataagt agtgaagctc atcattctca
     9481 tatcatttca gcaaataata gatttgatat catttttata tccaaaaact tagcaatgac
     9541 ctatcaggct tacccgcttt tcctatctca acacaggcta acttagctcc tatcaaatac
     9601 attttttaaa cataattaaa aacatttctg attatagttt attagatttc aacagaagta
     9661 tttttcctgc gtctcagctt ttgggtgtac cgttattttg aagttttgat agttgagtga
     9721 tatc
//
LOCUS       DATRNX010000069         9674 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRNX010000069 DATRNX010000000
VERSION     DATRNX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            61..1278
                     /locus_tag="VBC81_001389"
     CDS             61..1278
                     /locus_tag="VBC81_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6912430.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            1266..1667
                     /locus_tag="VBC81_001390"
     CDS             1266..1667
                     /locus_tag="VBC81_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912431.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            1651..1956
                     /locus_tag="VBC81_001391"
     CDS             1651..1956
                     /locus_tag="VBC81_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912432.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2145..2217)
                     /locus_tag="VBC81_001392"
     tRNA            complement(2145..2217)
                     /locus_tag="VBC81_001392"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Lys,seq:ctt)
     gene            complement(2499..3767)
                     /locus_tag="VBC81_001393"
     CDS             complement(2499..3767)
                     /locus_tag="VBC81_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6912433.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(3760..4251)
                     /locus_tag="VBC81_001394"
     CDS             complement(3760..4251)
                     /locus_tag="VBC81_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912434.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            4578..5048
                     /locus_tag="VBC81_001395"
     CDS             4578..5048
                     /locus_tag="VBC81_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6912435.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            5184..5642
                     /locus_tag="VBC81_001396"
     CDS             5184..5642
                     /locus_tag="VBC81_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6912436.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            5614..6084
                     /locus_tag="VBC81_001397"
     CDS             5614..6084
                     /locus_tag="VBC81_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912437.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(6123..6839)
                     /locus_tag="VBC81_001398"
     CDS             complement(6123..6839)
                     /locus_tag="VBC81_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6912438.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            6963..7421
                     /locus_tag="VBC81_001399"
     CDS             6963..7421
                     /locus_tag="VBC81_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6912439.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            7418..8371
                     /locus_tag="VBC81_001400"
     CDS             7418..8371
                     /locus_tag="VBC81_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6912440.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            8371..9111
                     /locus_tag="VBC81_001401"
     CDS             8371..9111
                     /locus_tag="VBC81_001401"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6912441.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            9108..9674
                     /locus_tag="VBC81_001402"
                     /pseudo
     CDS             9108..9674
                     /locus_tag="VBC81_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
BASE COUNT         3060 a         1616 c         2134 g         2864 t
ORIGIN      
        1 gatttagaaa ccaataaaaa atgaaaaaaa tatattgatg aagaaaaatt acaaatcgca
       61 atgtcagaat ataaaacaat ccatcccaac gccagattaa aggatcaata tgataacaca
      121 attggagttg tttcggaggt ttacgacaat acttctcatt ctggagaaca gatttatgct
      181 gtggttgata atcgtcatat tccaaaagaa gatgtaaaag aggtgacggt actttttcgt
      241 ggatcaacat ctcctcagga atttattaat aatcctgcag atgtgacttt ggattggtta
      301 gaaaatgaca ttcctatggc aagtaatatt tgggtaatga aagattttgg gaatcctcat
      361 aatttcagtg ctgtatcacc acagttgaaa gcttcatcag aacatttaaa agagattatg
      421 aaaaaatatc caaatgcaaa aataaacatt ggtggacatt cattaggagg tatggatgct
      481 caatatgctg ttgtagatat tacagataag aaagacttga aacggattaa ctctgtacat
      541 gtttataatg ctccagatat atatcctaca cttactaaag gacagaaaaa gacggcagat
      601 tcactgaaat ctaagattgt tgtttatgtt gatcctaatg attttattgg catggtgggg
      661 cgtgaaggaa aaaaaggaag tgaagactct gtaggaacag tctattatac agaaagtcca
      721 gatatcaact ggatagatca acacatgacc tatggctatc gtatggaaaa tggtcagatt
      781 aaagttattg aaacgaattt accaccggaa gttaggacta ctagaaaagc tatgggatct
      841 ttttataagg acaagaaaaa atttaaaaaa tctggtaagg gtctaagttc gcatgaaaaa
      901 atcttcttgg atgctgaaca agctgctgtt attgcaagtg gtttagtctc tactgctgaa
      961 actgctttag aggatatcaa gacatctgcg aaaacagctg ttggagaagc agaagaattg
     1021 tggaatacga ctaaatcgat tccttttggt attacggagc ttacagaagc agagttggca
     1081 gaagcttatg cagaaggtgg tgtcacttat gatagcatag taactaaaac tgatactcat
     1141 tttgatgaga aagtcaaaaa tgcaggagat ttagtaacaa catatacgac tttgaaaagt
     1201 gatattcaat caggtgttga aaccatgtta tctaaggata gtgaattagc tggagatttt
     1261 aaagcatggg aaaattagac gctaaagatg ataaattaag taacagtaaa aaggatttag
     1321 aaaagttgga agatgattac catcataaaa ccatagctat ttcagataaa ttctttgaac
     1381 tggaagataa acgatgtgag tttgagacga tgcttcagga aacttacgaa gcaacaagtt
     1441 atcatcttcg tcaggatgag atgattaatg aagagtcctt tatgaccatg aatcacatta
     1501 tagaagcttt tcaaagtgat tttgatacag aatatactaa agagaaaaga agattgactg
     1561 ccttagaaga agaaactaat caaaagtact ctaaaaagag acaattgctt gaagaaaaaa
     1621 tagatcattt aatgtcagaa aggagagact atgggaatcc ttggtgatta tgttttatca
     1681 gaagcctctg attatatcag tggaaaaaaa gatatcaagg ctttaaaaaa gaaacttgat
     1741 aaaatgttag tgacaggtgt ttatgctcca agaatcaagt ctaaacgtag aagtattgtc
     1801 tctacctatg atgcagagat aacgactact gcaacaaacg caaaagctag tataactgcc
     1861 attgcaggtc aaatagacac tgcaataaag ggacagttta gaactaaagt tgaaactgta
     1921 ttggataata acagtacaaa gtatgatgaa atttaaaaga catgggctaa ataatttagc
     1981 tcttttttta tactcaaaaa taagagaaat acaaaacaaa atacatattt atgcaagtaa
     2041 agagaaataa aaaaatcacc attttatctg tcaaaaaaaa ttggtgggca agtgtaaaaa
     2101 gagcgtcaaa aaagcctata ggtataggct ttgttttata agactgcccc ctacagggct
     2161 cgaacctgtg acccatagat taagagtcta ctgctctacc aactgagcta aggaggctag
     2221 gaagctgtat tggcaccgat gatccacttt ttgtattgat cccgcacaaa ataagcaggt
     2281 gggtaactcg cacctcttta agtggcttcc gcgtgttaac ggcttgcgca ctagagatga
     2341 cttcgtttcc cgaaatacaa agaaatagtc ggtcaacact tagatgtgga ttcgtatgcc
     2401 acagcaatgt ttctatgtct ttatgatacc actttttata aaattttcaa gcaaaattat
     2461 cgatttttga aatcgattgc gatttttaaa actggcaatc agtctgagag cttatcaatg
     2521 cgctctttat ttgaaccaag tgcacgttcg tatttaccag tttcattggc ttcaaaataa
     2581 ttatgtccta ctagtttatc tggtaagtat tgttgtttaa cccatttttc aggataggcg
     2641 tgagggtact tgtaatccct tgcattgccc aaggttttac taccgctgta atgtccatct
     2701 cttaggtgac gtggaatagg taaatttcct gaacgacgta aatcagctaa ggctgcatcc
     2761 atagccaaat aggctgaatt tgatttaggt gatagagcta aatccacaac aatgttagca
     2821 ataagaatac gagcttctgg aaaaccaatg cgttgggcag cttccaatgc agtcacagta
     2881 tgtatttgtg cttcggggtt agctaagccg atatcttcat aagcaataat ggtcaaacgt
     2941 cttgctaaac taggtaaatc tccagcttct actaaacgag cggcatagtg caggctggca
     3001 ttgacatcag aacctctaat tgatttttgt aaggctgata ggacatcata atgcccatct
     3061 ccgtttttat ccattgtaat ataactacgt tgcagactat tttccattgt ctctagacta
     3121 atatgacggc taccatcttc gttaggagaa gtagacatga ctgctagatc caaggaatta
     3181 taagcagatc gtaggtctcc atttgtggcg gtaacaatga aatctagcgc cccatcatca
     3241 attgtcacta aaaaaggaaa tccccgttct ttatcggaaa ttgctagttg aatggctttt
     3301 ttaatatcct cgttagaaag aggttctagc tcaaaaattt gtaccctact tctaatggca
     3361 ggagttactg aaaagaaagg attttcagtt gttgctccaa tcataataat attgccattc
     3421 tcaagtaaag gtaaaagaaa atcttgtttt gtcttgtcaa gtctatgaat ctcgtcaagt
     3481 aataaaacta agccaccaga aaatttagct tcttctgcaa tttcttgaag acgtttttta
     3541 ctatcaacag tggcattaaa agtacgaaag gcatatttag tagtaccagc aatagcagaa
     3601 gcaatagaag tcttcccaat acctggtggt ccatatagga tcatggaaga taacatatta
     3661 gctgcaacca ttcgatcaat aattttgcca ttaccaacga gatgttgctg cccaatgact
     3721 tcattaatat tgcgaggacg catacgtaga gcaagattgt cagccatgat tattctccaa
     3781 atcttttgcg aaaattaaag catcaacata atgattttca atgaaaaagg ctttagtgat
     3841 tcgagcctct tcggtaaaac cgtatttttg atagagggaa atggctctac tattgttgcc
     3901 catgacgtgc atggcaatct tgatgtaacc ttgagcttta gcctcagtta gcagagcttt
     3961 caagagagcc ttacctagcc cctgaccttg gtagggttca gcgatgagaa tgccgaaagt
     4021 ggcaacatgc ttgccagctg ggaaaggata gtagggacca aagtccaaga cgccagcgat
     4081 cttatcatcg atctcagcga caaagaagga catgccattt gccagattct tctccaagtc
     4141 actctcagtc cacttgtggt catagacagg agaggtaaga gcagtccagc cagtattttg
     4201 taaggtcaga actgcctcac ggtcgcagtt ctgcataggt ctgataatca taattgatga
     4261 tacaaaggga cggttcggtt tacgaaaatt ttcgtaaaga aatagggaac gacgaagtgt
     4321 cgtcagacac taggaagttc atcttttctt ttagaaaatt agtcccgtgt tcaaatcctt
     4381 tctatttttt atatagagtt tttctctcag ctgactcttt taacatagta tgaagtcagt
     4441 caaaaacgac tagtctttga gactattgta acataaaaat aagtaggaac taccttttga
     4501 ttaacctgag tctgtaacgt ttttcttcta tattcatgtt ataataagaa agaataaata
     4561 gaaaataagg acataagatg gctaaatttg gatttttatc ggtactagaa gaagaattgg
     4621 ataagcattt acagtatgac tttgccatgg attgggataa gaaaaatcat actgtagaag
     4681 tcacttttat attagaagca caaaacagct ctgctattga aaccgtggac gaccaggggg
     4741 agatgagtag cgaggacatc gtttttgagg actatgtact cttttacaac cctgtcaagt
     4801 ctcgctttga tgctgaggac tatctggtga ctatccccta tgagcctaag aagggcttgt
     4861 cgcgtgagtt tctggcttat tttgcggaga ctctcaacga ggttgccact gaagggttga
     4921 gcgacctcat ggacttcctc accgatgaca gtatcgaaga gttcggtctt tcttgggata
     4981 cggatgcttt tgaaaatggc agagcagaac tgaaagagac agagttttac ccttatccga
     5041 gatattagac ttgatggatt tgctaaaatg tggatataca ttggatgata tagaaacagc
     5101 cagaccagag gatatggaac gttattatcc ccctgaacaa atcagaaaat atggagcttt
     5161 aggaatagaa ttgaggttat tgcatggata tttttgatga gttatttgat ttctcaggtg
     5221 caaagatcgc tcttttctgc gagggaaaga ttttgaccag cctaagagat gattttccag
     5281 atttgcccta tgcaggcttt tgggacttac caggcgatgg tcgtgagggc gatgaaacac
     5341 ctttggaatg tctttttcga gaagttagtg aagaattaaa tctgacctta actcgaaacc
     5401 atattgattg ggtcaagact tataggggta tgctgaaccc tgataaatta tcggttttta
     5461 tggttggaca tatcagtcag aaagagtacg atagcattgt tctaggcgat gaaggacaag
     5521 actacaagct catgtccatt gacgaatttt taagccataa aaaggtcatt cctcagttgc
     5581 aggagaggtt gagagattat ttggaggtgg aagatgatta ctttacaaaa ggcggaagct
     5641 agtgatttag agaagattat tgccattcag agagctagct ttaaggcagt ctatgagaaa
     5701 taccacgatc aatacgatcc ctatgtggaa gaagttgagc agattcgatg gaaactagtg
     5761 gagcgaccag attgtttcta tcattttgtc cttgttgatg agacaattgt tggttttctt
     5821 agattggtta tcaaggacga ggagaagcgt gcttggcttg gtacggcggc tattcttccc
     5881 caatatcaag gacagggcta cggctcagca gcgatggctt tattagagaa aacctatcct
     5941 aagcttacaa agtgggattt atgtacgatt gcgcaagaaa aactaatggt cagtttttat
     6001 gaaaaatgtg gttatcattc aacccatacc gaacctgagc aagaaggaat ggatatggtc
     6061 tatatgacaa agaagataga ataaaaaaag aaaactagat gattgtctag ttttctgata
     6121 gtttattggc aatagtaagc aatagcttgg ctgacaaatt cacttgttcc tgctccacca
     6181 gctttgtcaa ttgattgact aaaccggtca tctgattgat acatttgtcc tagtccagca
     6241 aggatttcat tggtacaggt gtaaaaatgc tgagagatat agtcttgtag ccgtttcaca
     6301 cacatttgaa cagattcatc agatacattt tgtgtcttta attgcccaaa ttgtaccatt
     6361 atttttgcca tttcttggct aatttgtgca aagtcatctg aagcatgttt ttgagcaaat
     6421 tcttggtagg ctgctgtttg tccccatttt tcttttgctt cagcttgtaa ttgttctaat
     6481 tctgttttat tataaacatc gaaattcata taatcatatc ctttttcttg taatcgtttc
     6541 gcatgggtga taacctgctc aagatgcgat cttttccatt caagcaattt gatttgttgg
     6601 gctaataagt cttgcctttc ctcttttgct tgctcaagca ggtatttgat atctttgaga
     6661 gggaattcca actcacgaaa tagcaaaatt tcttgcaggc gaatcaatgc ttcatcgtca
     6721 taaagtcgat agcctgcttc ggatagggcg gtaggtggga ataaaccgat tttatcatag
     6781 tgatgtaagg tgcgcacact tacaccgctt aagatactga cttctttaac tgttttcatg
     6841 gtaataacct cctctcactt acatagtata aaggataacc taaggtcagg gtcaagcaaa
     6901 aaataaatta ttttgaaaag tatggttaat catattgatt tgaagataaa ggagggatag
     6961 ttatagaaat agttgagcta ctaagggaat ggtattcttc ctttgaagtt aaagaagaaa
     7021 agtggaatga aaaatatgaa ggtatcctca taagaattga aggcgatgga aactataaaa
     7081 ggtgtcgttt agctcataag acagctagca agatggggta ttttacagta ttttggcaga
     7141 aagatggaaa tggtaaaaat attccttttt atgaacaaga tgaagtggaa gatttgatta
     7201 ttgtcattaa agatggtagg aggaaaggtt tgttcatcat tcccaaagag gttgcagtga
     7261 gtaagggtat tctttcatct gctaactctc aaggaaagat ggctatgcga ttttactctc
     7321 cttggtgtag cgatttaaat agaacagctt tggtgacaca gcgatggcag ttgaactatt
     7381 ttattgattt aagtaaaaat aatgaaggag ttactacatg aacacatgga atgaactaac
     7441 ggttcacgtg aatcgtgagg cagaggaagc tgtctcgaat ttattgattg aaactggcag
     7501 tcagggggtt gccattagcg actcggctga ttatcttgga caggaggatc gtttcggcga
     7561 gctttatcct gaggtggagc aatctgatat gatcgctatt actgcctatt atcctgatac
     7621 gcttgatatt gaggcggtta aggctgatct ggctgatcgt ttggctaatt ttgaaggctt
     7681 tggtcttgcg acgggtagcg tcaaccttga cagtcaggaa ttggttgaag aggactgggc
     7741 tgataactgg aagaagtact atgagccagc tcgcattacc catgatttga ccatcgtgcc
     7801 gtcatggacg gactacgagg ctaaggctgg tgagaagatt atcaagctgg atcctggtat
     7861 ggcttttggg actgggactc acccaacgac taagatgagt ctttttgcct tggagcaagt
     7921 gcttcgtggt ggtgagacgg ttatcgatgt ggggactggt tctggcgttt tgtctattgc
     7981 tagctctctc ttgggtgcca aggacatcta tgcttatgat ttggatgatg ttgcggtgcg
     8041 cgtggctcag gaaaatatcg atatgaaccc tggtactgat aatattcatg tggctgctgg
     8101 tgacctgctc aaaggtgtcc agcaagaggc agatgtcatt attgccaata ttttggcgga
     8161 tattttgatc catctgacag acgatgccta ccgtttggtc aaggatgagg gttatctcat
     8221 catgtctggt atcatttctg aaaaatggga catggtgcgt gagagtgctg aaaaagctgg
     8281 tttcttcctt gagactcaca tggttcaagg tgagtggaat gcctgtgttt tcaaaaagac
     8341 agatgatatt tcaggagtga ttggcggcta atgcaacagt attttgtaaa tggcaaagca
     8401 gatgactttg tgaccatcga ggacaaggat accatcaagc acatgttcaa tgtcatgcga
     8461 ttgacagaag atgaccaagt ggtcttggtt tttgatgatg ccatcaagcg tctggcaaag
     8521 gtggtggaca gctctgccca tcgcttccaa attctagaag agctagacag caatgttgaa
     8581 atgcctgtcc aagtgaccat cgcctcaggt tttcccaagg gagacaagtt ggattttgtg
     8641 acgcaaaagg caacagaatt gggtgctgcg gctatctggg gattcccagc tgactggtca
     8701 gtcgtcaagt gggacggcaa gaaactagct aaaaaagaag ataagctggc aaaaattgct
     8761 ctgggcgcag cggagcagag caagcgtaat cgtcttcctc aggttcgttt atttgagaag
     8821 aaggctgatt ttcaagcgga gcttgcaggc tttgataaga tttttatcgc ctatgaagag
     8881 tctgccaagg agggtgaaat gtcagcgtta gcccagaatt tgcagactgt aaaagcaggt
     8941 gacaagctcc tcttcatctt tggaccagaa gggggcattt ctccaaaaga aatcgcagct
     9001 tttgaagagg ttggagctat caaggtgggc ctgggtccac gcatcatgcg cacagaaaca
     9061 gcaccgctct atgctctaag tgttattagt tactcagcgg agttattatg attattgcta
     9121 ttcaaggtga tatggctgta gggaaaacca cagctttgaa aatcattgag aaagtttacc
     9181 cgaaaatcgc tattagtttt gaggatattt caccagtagt tgataagatt aataaacatc
     9241 agcttgataa caataaatta gacgattatt gtgaaattca acaactattt attcagcatg
     9301 agcgagatag atacgaactt gttagagata atgaaatagt gttaatggat tatggagctg
     9361 aggaaattgc tttctataca caatactttc caaaggtggc gggttatagt tgactttacc
     9421 ttctaatttg ttactagagg taaataggct aaaggaatgt tttgctgatt atattatttt
     9481 tctagatgca gatgagacgg tcctatacca acgtcaaaaa aatgatttta atagacggcg
     9541 tcattttttc aaatggtata ctcatcacct gttgcctgct aaaaaacatt atttagacac
     9601 attttccaat gttattcata ttaatacaaa tcatttaaat gctgaagaag tggctgaaaa
     9661 aatcattgac atca
//
LOCUS       DATRNX010000070         9663 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRNX010000070 DATRNX010000000
VERSION     DATRNX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            28..675
                     /locus_tag="VBC81_001403"
     CDS             28..675
                     /locus_tag="VBC81_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6912442.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(715..1404)
                     /locus_tag="VBC81_001404"
     CDS             complement(715..1404)
                     /locus_tag="VBC81_001404"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6912443.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(1414..1683)
                     /locus_tag="VBC81_001405"
     CDS             complement(1414..1683)
                     /locus_tag="VBC81_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912444.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(1683..2237)
                     /locus_tag="VBC81_001406"
     CDS             complement(1683..2237)
                     /locus_tag="VBC81_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6912445.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(2258..3637)
                     /gene="glmU"
                     /locus_tag="VBC81_001407"
     CDS             complement(2258..3637)
                     /gene="glmU"
                     /locus_tag="VBC81_001407"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6912446.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(3881..4294)
                     /locus_tag="VBC81_001408"
     CDS             complement(3881..4294)
                     /locus_tag="VBC81_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6912447.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(4307..4684)
                     /locus_tag="VBC81_001409"
                     /pseudo
     CDS             complement(4307..4684)
                     /locus_tag="VBC81_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(4773..5729)
                     /locus_tag="VBC81_001410"
     CDS             complement(4773..5729)
                     /locus_tag="VBC81_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6912448.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(5726..6178)
                     /locus_tag="VBC81_001411"
     CDS             complement(5726..6178)
                     /locus_tag="VBC81_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6912449.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(6175..6420)
                     /locus_tag="VBC81_001412"
                     /pseudo
     CDS             complement(6175..6420)
                     /locus_tag="VBC81_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(6417..7115)
                     /locus_tag="VBC81_001413"
     CDS             complement(6417..7115)
                     /locus_tag="VBC81_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6912450.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(7108..7344)
                     /locus_tag="VBC81_001414"
     CDS             complement(7108..7344)
                     /locus_tag="VBC81_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6912451.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(7440..8585)
                     /locus_tag="VBC81_001415"
     CDS             complement(7440..8585)
                     /locus_tag="VBC81_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912452.1"
                     /translation="MKIKEHKKKNGAIVYRASIYLGIDQVTGKKAKTSVTGRTRKEVK
                     QKARHAQDEFISNGYTVTKVVPIKNYQELAELWLENYQLTVKPQTFIATKRMLYNHLI
                     PIFGTMKVDKLTVSYIQRFINDLSNQLVHYGVVHSINRRVLQYGVSLQLLPFNPARDV
                     MLPKVPKKENKAIKFIAPEDLKALMAYMEKLANKKFSYFFDYVLYSVLLATGCRFGEV
                     VALEWSDIDLENGTISITKNYSRLLKLIGTPKSKAGVRVISIDKKTINLLRLYKNRQR
                     QLFIETGTRVSAVVFSTPLKEYQNMATRQESLDRRITEVGIPRFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHATLAMTMDIYGHLSMDKEKEAVSYFEKAINNL"
     gene            complement(9301..9588)
                     /locus_tag="VBC81_001416"
     CDS             complement(9301..9588)
                     /locus_tag="VBC81_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEO6912453.1"
                     /translation="MLRRVNHVKNVLAHGEFAEWIENKIGIHYREANRMMTVAKQIPN
                     VSTLKYLGATAKHVNGVAKRKQNFLSQISLIPTNPQLPHQTIINTYLYWQP"
BASE COUNT         2992 a         2016 c         1441 g         3214 t
ORIGIN      
        1 ttttaagaag tataagaaag actgaatatg actccaaata aagaagatta cctaaaatgc
       61 attcatgaat taggtgaaaa aaacaacaaa atttccaata agaaaatcgc tgaaatgatg
      121 caagtctcgg ctcctgccgt gtcagaaatg attaaaaaaa tgataagtga acaactgatt
      181 gttaaagata aagatttagg atattatctt accaaacaag gtttattagt tgtttctgac
      241 ctttaccgta aacatcgtct agtagaggta tttttagtta accatttgca ctatactgct
      301 gatgatattc atgaagaagc ggaagtttta gaacatactg tatcaacaac ttttgttgat
      361 caattagaaa agttgttaga ctttccacaa ttttgtccac acggtgggac tattcctaaa
      421 aagggagagt ttttagtaga aattaaccaa atgacattgg accaaattag tcagcttggc
      481 acatatgtta tcagtcgtgt tcatgatgat tttcagctac tgaaatacct agaacaacac
      541 cgtttgcata tcaacgatac cattgaattg acacaaattg acccttatgc aaaaacttat
      601 cacatcacct ataacgatga aaatctaaca atccctgagc gtatcgcaag tcaaatatat
      661 gtatctgcta cataaataaa aaagtccgtg tattcattag atacacgggc ttttttataa
      721 tgctttcaaa aatgccatta atacttgggc agagcgttta ccagcttcaa tgataaattc
      781 atcaaatgtg atattggcat catgagctgc agtatcactc atagcacgta ccaccacaaa
      841 gggctttcct gtagcttgag cagcttgggc aattgctgca ccttccatct cgacagctaa
      901 aacctgtgga aaatgcccct taataacatc aatcttttcc tggccagcaa taaaactatc
      961 cccagttgca attaaaccaa ctttactagt catttcttct ttagataaaa ctgcttcaaa
     1021 agtggaaaca aaagtcttat ccgaatgaaa ataaagtggc tgcattgaca tttgtccata
     1081 gtcataacca aaggcggtta aatctacatc gtgataaact aatgtatctg cgacaacgac
     1141 gtctcctaca ttcaatcctg ttgctactgc accagctgat cctgtattaa taatagcatc
     1201 aactttaaag ctttctacta aaatagcaac actcatcgct gacatgactt tcccaacacc
     1261 agactgtact aaaacaagct catgttcacc ataacgtcca ctgtaatata catttgataa
     1321 tactgtctct tgagatttat cctcaagatt ttccacaagg agttttaact cttcctccat
     1381 ggctgcaata attccaattt tcatgatatt tccttataat ttaaaaattg cataaaacaa
     1441 gagagccaac aaaataagca aaatgaataa aactaaattc aacttacgtt gaaattcatt
     1501 tcgttttgca ttctcaatcc ggcgactctt gtagatatgt ttaccgttct cgtcagttag
     1561 ttgctccatg ccatcttctg tagaaataat tttagtttct tgatctaaga ttgtttgacc
     1621 tgaaactttt tcacctcgat ttgaacgttc aatcatatca tctgttaaca atggttttcc
     1681 cattaagaat gacctcccat ttttaaaccg taatattgta aagcaatgat cgttttcgca
     1741 tcttgaatca tgcctttttc taccatttgc atacaatcct cataacttaa ctctagaagc
     1801 tcaaggacct catcatcatc ttgtggccta ggattttcaa ctttttgtaa atcagtagct
     1861 agatagagga cgattttctc attacaaaaa ccaatagcag tgtagaaact ataaagaatt
     1921 tcaagattcc ctgtgtagcc agtctcttct tctaattccc gaagggcagc ttcttcctta
     1981 gaacctgact cacccgtctc aagtttccct gctggaattt cgtaagaaat accttcaata
     2041 gccttacgat actgtttcac taaaacaatt ttatgctcag gagttaccgc caaagtagca
     2101 acagctcctc catgaaaaac aagttcacgt ttggattgtc ccaatccatt tggcaactct
     2161 acatcatcaa cagctacttt gataatttgt ccatcaaaaa cagtttgacg attaattgtt
     2221 ttttcttcaa aatccataag aacctcccaa ataagtgtta cttttgtgaa ggatgatgtg
     2281 gttttttatt ggcatatcct tctttattga cttgacgtcc tcttccaata gctatgctat
     2341 caattggaac attatctgtg atggtagatc cagctgctgt taaagcatta tcaccaattt
     2401 caagtggtgc aatcagagta gaattacttc cgataaagac attacttccg atttctgttt
     2461 taaatttatt ttgtccatca taattgacag taatcgtccc tgcgccaaag ttaacatcac
     2521 aacctacctc tgcattgccg atataagtta aatgacctgc cttcgtattc tcacctattt
     2581 gagaaccctt tacttcaacg aaattaccaa tatgaacacc ttttgcaaga cttgttcctg
     2641 gtctaatatg cgcataaggg cctacggtta ctccatcaga aataatggat tcttcaacca
     2701 tcgaattagt gatggtaaca tcatttccga cttgagcatc tactaaataa ctaccattgg
     2761 ttaataaagt acctttacca atcttcgtct gtccttttaa agtcacatta ggctcaatta
     2821 cagattcttc tccaatctca acatcaatat caatataagc ggaatcagga ttaaccactg
     2881 tgaccccatt aaccatatgt tgacgtgcaa ttcggtgacg catcactttt tcagcagttg
     2941 caagtgctac acgatcatta actcctagac tttcgtcaaa gtcacgaagt ttataggcac
     3001 ctaccttttt acccgcttct ttgaaaattc caataacatc agttagataa tactctcctt
     3061 gagcgttatt tgtattaatg tctttaagtg cttcaaatag actttgatta tcaaaaacat
     3121 aagttccagt attaatctct ttaacctgct gctcaaagtc attagcatcc ttttgttcta
     3181 caattttagt aacttcatca tctgaattac ggataatacg accatatcca aatggatttg
     3241 cagcatcggc tgttaagatt gttgcaacgt ttttatgatt gacatgaaaa tcaattaagt
     3301 tttttaaact ttctccagta atcaggggtg tatcacctgc aataactagt gtatgtcctt
     3361 ttgatgtcgc taattcttct tccgccatca ttacagcatg gccagttcct agctgttctg
     3421 tttgcattac aaactctgat ttgtcaccta aaacatcacg tacaagctcc gccttatgtc
     3481 caataacagt aacaatttta cttggctcaa tagcttgcac actacggaaa acatgctcaa
     3541 gcatggtaat accagatact ttatgcataa cttttggtaa atcagatttc atgcgagttc
     3601 ccttacccgc agctaaaata atggcataat ttgacatatt aattcctcta tctctgaata
     3661 taattcaacc tattatatca taatgccttc aacttatcac taatacccct ttaaatccct
     3721 attacaatag actttagcta aaatagctta cttcaattag gaataaaata gctacctttt
     3781 ggtagctatt caatacatga aatccgttaa ttttttcttc tctgtttgca atcagtattt
     3841 ttataaatct atcattgcaa tgctcagatg gtaaaaacga ttagtttaac tctgttgaaa
     3901 tttgccacat cacaccaaat tggtctacta agtttgcgta agcaggattc cagaaagttt
     3961 cttgtaactc taactgaact tcttgtgcac tcttctttaa tttttcataa atttcacgcg
     4021 cctcctctac gctacccaca ataatagcgg ctgtaacatt tttaccagct tgatacacga
     4081 aatctggatt ttcatccgag atttgaagac gaatgccatc aaaaataagc tgagcattaa
     4141 tgattaaatc cttgcggtct tcaagacagt tggggtccat ctcttcccat gtcatcatat
     4201 tcactaaatc cgcttgaaag acatctttat aaaaatcaac cgcttgtcgc ccatttccat
     4261 ttgttacgat gtatgtttct agtgctttaa ccataagttc tcctttttat tttacttcac
     4321 ggattgcaaa gtaattattg tcattatcgg aaaaattaaa aactctcata ctccccatat
     4381 caacaatagg atttgtcata acctcatttg ctgttaattc ttgatatgta ctgtcaatat
     4441 cagtagtttc aaataaaata gagggagttg ctaaatctag ctcaggactc atttgagcta
     4501 taattgcttt gtcatgtaag acgaagctaa cttcactatc caacttcggt gcaactatat
     4561 aggttacata atctccttgt gtctattttt caacacgctc aaaacccacc ttgtttttcc
     4621 aaaaatctgc tgatgcttca acattgctaa cgtagagcat tacctgtcca attgatgaaa
     4681 tcattttatt atctcacttt cggttttatt actaattagt ataacataca gtatttaaaa
     4741 taagtaaaaa atgactcaaa agagtcattt acttatttcg ttaccatatc tagaactttc
     4801 atcaccgttc tactatgttc taaagcttga gcagcacgct taaaatctaa attgctaata
     4861 ataccttcaa aaataacaaa ttcatcgtgc atgcggtgcc tatcaccatt taattgataa
     4921 acatgacttt cttgtccatt catagttaat gaaattttag gcatagtatt agttggccct
     4981 aaaatcgcta tagacccctt atcaccttga atagtagatt ttacttctgc actacaatct
     5041 tttgctccaa tacagactgt tttaaagtgc ccatagtcta ggactagaac tcctgaagta
     5101 tcaataccac gctctatatt tggtaaatac tgtgctgtta taggttctcc aaaaagtcca
     5161 attactaaat gtaaattata aatattaagg tctctcaacg cacctccacc catttcagga
     5221 ttgaaagctg gtgctatttc cccacgttta aaggcatcat aacgcgatga atattgggaa
     5281 taattacatt ctacaatttt aatatctcca aggttgctta aatgttcttt tacaaggtca
     5341 aaattaggta aatattgatt tgtaattgct tcgagtaata ttagttgcct agtattggca
     5401 atagatacaa gttcttctaa ttgacttgct tctaatgtaa atggcttctc acaaatgaca
     5461 tgttttcctg ccaagagtgc ctccttagca tagtcaaaat gcaagtgatt cggcagtcca
     5521 atatagactg tgtcaatctc ctcattgtcc aagacagcct tatattcact cgtagctaga
     5581 gacatgttat attccttagc tagatcttta gctgtctcca aacttctaac tgttgataag
     5641 atagcaacta gatcaatacc ttcaattttc tgcaagacag gcaaaacttc cttaacaatc
     5701 atacctgtcc ccaaaaccgc taatttcatt cgttaatctc ctttgggtaa accacttgga
     5761 attcctctaa aactatccta gagtcctcac taaacattaa accaaactcc tcaaaataag
     5821 gtttaaagaa atcaatgtga gcttttctcc accattctaa agttttatct ccttctcctt
     5881 ctttaaaagc atggtctgca gagacatctt taaaagggac aatatcaact ttagttattt
     5941 caataataca gatagcttca tcattgctat ccaaaataat atcatatgaa ccaacttgtg
     6001 gtaacccttc ctcatctaat tcatataaat cataggcaga ggccgttgca gtttttgtcc
     6061 catctacaac taactttgcc agaagatctg gttctgctcc aaattgccat gcgtctatct
     6121 catcaccgat tgaaggattc aaagatttaa acttattcca taattcttgt gccttcatat
     6181 tacctccgaa gcaattccca caacaaactt aactgcgtta ctcatttttc tttgtctttt
     6241 aaatccttct ttaacatcta aaatataaac agtttgacca atccaaacac gtaaataaac
     6301 agattttatt aaaatatcac ctgatattcc tttaacttcc cgctcagtac catcagatgc
     6361 ttcaaattcc gtacgtaata accaagtatt accacaacca aattcaatct attttctcat
     6421 ttcaaactcc agccaccatc aattttgaca atttcacctt gcattgatct cgcttttcct
     6481 gaagcaagaa atccagttaa ttcagcaact tcacttggtt tagtccaacg accaattggc
     6541 gtctcactag ctacccattc tgctaatcca ccaggttcaa aatcactagc agtcatagca
     6601 gtttgtacag cacctggcgc tataccaaaa atttgaatac aatcttttgc gtaatccaaa
     6661 gccaattgac gtgtgaatcc cgcaagggca tgtttagaac tagtatatgc agctccaccg
     6721 ccacccgcta taaaacttgc tattgagcac atattgataa taatacctga cttcttttcc
     6781 accatacgtc taagataatg acgtgttagc ctaacagtca cgaaaaaatt aatatcaaat
     6841 aggtgttcca gttcttcatc acttacttct agaagtggct tgtaggcatc tagaatacct
     6901 gcagtattac acaaaatatc aacagtcggt accattgtga atagtgggga taaatcagag
     6961 gataaatcca actttataaa gttaaaatta ccatttaagt tcggtttatc cgacttatcc
     7021 acaccatata cttgataccc ttgtttcaaa aaatattgag cctgtgcata accaattcca
     7081 ctagcacagc ccgtcactaa aacaacttta gtcattgacc tcgatccaat cctctgctaa
     7141 aacatcacat ggtgttggtg cccacatgga aaaaccttca ccttcaccag taacattaat
     7201 aagaaaataa ggtgtagcct gtagtacttt cccattaact tcaagggtat caaataactg
     7261 aacataattt tcagcgtccc cccaacctgt acgtacatat tttttctttg cttttaaacc
     7321 tggtaaaatt tcttcaaatg tcatattgtt ctcctttatt gtctataaac gttgatttaa
     7381 taggcttttc aatgagccta taaacaccta aaaataaatt tgttcacctt tttgtggact
     7441 tacaggttat ttatagcttt ctcaaaatag gaaacagctt ccttttcttt gtccatggaa
     7501 aggtgtccgt aaatgtccat ggtcatagcc aaagtggcat gacctaatct gtattgtagt
     7561 tctttataac taataccagc atttagcaat aaactagcgt gagtgtgtcg gaaagcgtga
     7621 aaggtaaatc tagggatacc aacttcagta atgcgcctat ctaggctttc ctgtctagta
     7681 gccatgtttt gatattcctt tagtggtgtt gaaaatacca cagcagaaac acgcgtccca
     7741 gtctctataa ataattgtcg ttgcctattc ttgtaaagtc gtagcagatt gattgttttc
     7801 ttgtctatgc ttatgaccct tacccctgct ttgctttttg gcgtaccaat taactttaat
     7861 aacctactat aattcttggt tatgctgata gttccattct ctagatcgat atcagaccat
     7921 tctagggcta ctacttcccc aaatcggcaa ccagtagcaa gtaaaacgct gtatagaaca
     7981 taatcaaaga aatagctaaa tttcttattg gctagctttt ccatgtaagc cattaacgct
     8041 tttaaatctt ctggagctat gaacttaata gccttatttt ccttttttgg tactttaggc
     8101 aacataacat cacgcgcagg attgaatggc aataactgga gagatacgcc atactgtaaa
     8161 acacgtctat ttatcgaatg aactacaccg taatggacta actgatttga taagtcgttt
     8221 atgaagcgct gaatatagct gacagttaat ttatcaactt tcatagttcc aaaaataggt
     8281 attaagtgat tatagagcat tctcttagtt gctataaatg tctgaggctt taccgtaagc
     8341 tgataatttt ctagccataa ctcagccaat tcttgataat tctttattgg gacaacttta
     8401 gtaaccgtgt agccattaga aatgaattca tcttgcgcgt gccttgcttt ctgcttaact
     8461 tcttttcgtg tcctacctgt tacgctagtt ttagctttct tacccgttac ttggtcaatg
     8521 cctagataaa tactagcacg ataaacgatt gcaccgtttt tcttcttatg ttcttttatt
     8581 ttcatgatga taaacctttc catcagcagg caagctatta ttaaaagggt tttagattag
     8641 tttatatcat ggtataataa cggtatcggg ttaatatccg aaatattata aatagaaaat
     8701 tgtgatataa tattgttgtt ttttaagacc ttgcgagcgt ggaaaacttg cgaggtcttt
     8761 ttttgtttgg ttttagtaag ttggtaaatt tttagatagc ccagaaaagc tggctgtctc
     8821 ttggctattg ttagccgtat atatcaaaaa ttgtaattca ttcatggcta ttcctttaag
     8881 gttaccccca tcataaaaaa atgttgttca atccaagttt tgaaaccggt gggaagggtc
     8941 aactgtccaa atatttgctt tttttctcac acgacccccc accccgaaga taagaatact
     9001 aaattatcac atcattaaag agacaaacgt ttctaactgc cattgctttt attggaggag
     9061 tagttgcata agttggaaat cgattatact tttgcaaagt gaatgatgaa aatagctcat
     9121 gaattaccaa atttcccgac gttgggaaat atcggaacag aagctctcta ccttattgcc
     9181 accctaccag ataaccaaaa gtaagaacag cttgaataac ctattgcttc ctatggcgga
     9241 aggctcttga acctaaatgc gaccatgtgg tcgtatttga catgttaatg ctaaggaatg
     9301 ctatggttgc caatataaat aagtgttaat tattgtttgg tggggtagtt gagggttggt
     9361 cggtatcagg gagatttggg agagaaaatt ttgctttctt tttgcaactc cgttgacatg
     9421 ttttgctgta gcccctaaat atttcaacgt tgaaacattt ggaatttgtt tagctaccgt
     9481 catcatcctg ttagcctccc tgtaatggat accaatttta ttttctatcc attcagcaaa
     9541 ctctccatgt gctaaaacat tttttacatg atttactcgt ctgagcattt attatttatt
     9601 taatcaaaat ctatcttatc tccctcagta tagattttat atccgtagga ctccaaattt
     9661 ttt
//
LOCUS       DATRNX010000071         9607 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRNX010000071 DATRNX010000000
VERSION     DATRNX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..291
                     /locus_tag="VBC81_001417"
     CDS             <1..291
                     /locus_tag="VBC81_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6912454.1"
                     /translation="YFLIQTKSPKGYLQSNKPFYFTVNKDSVSKAQFGNYNINGYIMD
                     MQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFYITAIRFVFKRM"
     gene            316..1140
                     /locus_tag="VBC81_001418"
     CDS             316..1140
                     /locus_tag="VBC81_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6912455.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            1140..1862
                     /locus_tag="VBC81_001419"
     CDS             1140..1862
                     /locus_tag="VBC81_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6912456.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            1862..2920
                     /locus_tag="VBC81_001420"
     CDS             1862..2920
                     /locus_tag="VBC81_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6912457.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            2922..3857
                     /locus_tag="VBC81_001421"
     CDS             2922..3857
                     /locus_tag="VBC81_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6912458.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            3866..4813
                     /locus_tag="VBC81_001422"
     CDS             3866..4813
                     /locus_tag="VBC81_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6912459.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            4815..6179
                     /locus_tag="VBC81_001423"
     CDS             4815..6179
                     /locus_tag="VBC81_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6912460.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            6179..7603
                     /locus_tag="VBC81_001424"
     CDS             6179..7603
                     /locus_tag="VBC81_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6912461.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            7600..8448
                     /locus_tag="VBC81_001425"
     CDS             7600..8448
                     /locus_tag="VBC81_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6912462.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            8438..9586
                     /locus_tag="VBC81_001426"
     CDS             8438..9586
                     /locus_tag="VBC81_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6912463.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
BASE COUNT         3128 a         1413 c         1837 g         3229 t
ORIGIN      
        1 tatttcttaa tacagacaaa atctccaaaa ggctacttac aaagcaataa accattttat
       61 tttacagtaa ataaagatag tgtttctaaa gcacaatttg gtaactataa tataaatggt
      121 tatataatgg acatgcagta caacaaacaa gaatacaatg cacttgcaaa cactccaaaa
      181 ggtcaaaaaa caaagaaaac tttcaaaccc atgaccctag tgattctttt tgcagtagct
      241 ttatttattt tctatattac agctataaga tttgtattta aaagaatgtg agttaactgt
      301 tagacagagg taattatgaa agaaatgacg gtctcagaaa taagagaagt tcaacttgaa
      361 atgttagctt atattgataa agttgcgcgt gataataaaa ttgaatattc tttaggcgga
      421 ggcagcctac tgggagcaat gagacataaa ggattcattc catgggatga cgatattgat
      481 ttgatgctag aaagaagcca gtatgagcgt ttgatgaaag cattagctga tgctaataat
      541 agcgatttta aattgctaca tcattctgtt gaaaaaaatc tatggccatt tgccaagctg
      601 tatcacacaa agtcaatgta cttaagtaaa acagatagaa ttcatccttg gacagggatt
      661 tttattgata tcttcccact tgaccgatta ccagagagtg cagaagaacg tcaaagattc
      721 tttaaaaaag ttcactctgc tgctgcaaat ttgatgtgta cgacttaccc caattttgct
      781 agcggttccc gaaagcttta tgcaaatgca aggctaattt tggggctacc ccgattcatc
      841 gcttatcacg gccaagctaa aaaacgtgct gaaattgttg atcaagtgat ggaaacatat
      901 aataatcaag aggttcctta catgggttat accgattcaa gatatcgctt gaaagaatat
      961 tttccaagag aaattttttc tgagtatgaa gatgttatgt ttgaaaacat taaaacccga
     1021 aaaattaaaa atgagcatgc ttatctgaat caattatatg ggggtagtta tatggaatta
     1081 cctccagaaa gtaagagaga aagtcatagt tattacactt ggtactggaa agaaggttaa
     1141 tgatgaatat tggagttatt tttgcaggtg gtgttggtcg tcgcatgaat accaaaggta
     1201 aaccaaagca attcctagaa gtgcatggta aacccattat tgtacacaca attgatattt
     1261 tccaaaatac agaagctatt gatgctgtag tagttgtctg tgtatcagac tggctagatt
     1321 atatgaataa tctggtagag cgtttcaacc ttacgaaagt aaaagcagtt gttgcaggtg
     1381 gagaaactgg ccaaatgtca atttttaagg ggcttgaagc cgctgaacag ctggctacag
     1441 atgatgccgt tgttttaatt catgacggag tacgtccatt gattaatgaa gaagttatta
     1501 atgctaatat acagtcagta aaagaaacag gaagtgcggt aacttcagtt cgtgctaaag
     1561 agacagttgt tctagtcaat gattcttcta aaatatctga agttgttgat cgtactcgtt
     1621 ccttcattgc taaagcgcca caaagttttt atctatcaga tatcctttct gttgaacgtg
     1681 atgccattag caaagggatt actgatgcaa ttgattctag tactttgatg ggtatgtaca
     1741 atcgtgaact cactattgtt gagggaccat atgaaaatat caagataaca acaccagatg
     1801 atttttatat gtttaaagct ttgtatgatg ctagagaaaa tgaacaaatt tatgggatgt
     1861 aatgatgatt aaatttagta ataaaattgt acaagaagat ttagcactta ttagtaacca
     1921 aaattatatt aattgggaaa gaattgctaa taaaggcgtt ttaataagtg gttctaatag
     1981 tatgttagcg agttatatgg tatttttact agcctatctc aatgagacaa gaaattacca
     2041 aacgcaaatt attgcgacag ctagaaacat tgaaaaagcc cgtgataaat tttcagattt
     2101 agttgggaaa gattatttca ccttaattcc ttatgatgta gaagaacgcc tagagtatga
     2161 tggaaaagtg gattacatta tccatgcagc aagcaatgct agtccaactg ctattttgag
     2221 taatcctgtt agcatcataa aagctaatac aattggtaca ttgaatttat tagactttgc
     2281 aaaggaaaaa acaattgaaa acttcttgtt cttatcaacg cgtgaagtat atggaacatc
     2341 tattaaagaa gtgattgatg aagaagctta tggtggattt gatattttag ctactagagc
     2401 ttgttaccca gaaagcaaac gtatggctga aactctactg caaagttact atgatcaata
     2461 taaggtaccc tttactattg ctcgtattgc acattcattt ggtcctggta tggagcttgg
     2521 taacgatggt cgtattatga atgatctttt atcgaatgtc attgatggca aagatatcgt
     2581 tttaaaaagt agtggtacag cagagcgtgc tttctgttat ttagcagatg ctgtatcagg
     2641 gttatttact attttattaa atggtgaagt tggtcaagct tacaatgtag caaatgaaga
     2701 tcaacccatt atgattaagg atttggcaca aaagttagtt gatctctttt ctgataaaaa
     2761 tatttcagta gtattcgata ttcctaaaac tatgagtgca ggttatagca aaatggggag
     2821 aacacgtctc actatggcca aattagaagc gttgggatgg aagcgagaag tttctcttga
     2881 atctggaata ttaaaaacag tacaagcatt tgaggagtaa tatggtaaag acagcagttt
     2941 taatggcgac atacaatggc gaaaaattta tatctgaaca acttgattca attcgccaac
     3001 agacattaaa accagattat gtattattga gggatgattg ttcaacggat gaaacagtca
     3061 atgtcgtcaa taactatatc gcaaaacatg agttagaagg ctggaaaatt gttaaaaacg
     3121 acaaaaactt aggctggcgt ttaaattttc gtcaattact tattgatgtg ttagcctatg
     3181 aggttgacta tgtctttttt agtgatcaag atgatatttg gtatcttgat aaaaacgaac
     3241 gacagtttgc cattatgtca gataaccctc aaattgaggt tttgagtgca gacgttgata
     3301 tcaaaacgat gtctacagaa gccagtgttc cacattttct aactttttct tctagtgata
     3361 gaatcagtca gtatcctaaa gtatatgatt atcaaacatt ccgtcccgga tggaccattg
     3421 ctatgaagag agattttgcg caagctatcg ctgataattg gacttttgga gctaatattt
     3481 cgcacgataa tcttatcgag acaactgcca gtattattgg aaatggttat aaccttaacg
     3541 aaagcgtagg agttcatatt cgatatggag gaaacgcaag tggtaatagc aaatttgttt
     3601 tgaattggaa gtcgcctaaa acacaacata tcacaggatt aaaaacattt gttgattttt
     3661 acgaaaccat gcagaaggtt gtcaacaaat atcaaaatga tgtcgcttta aatcaacgct
     3721 tagcctttga aaaagaaaga ttggaagctt ctaaattgtc attaattcat aatagtgctt
     3781 ttgtgattaa aaattggcga ctctataact ctaatagtgg acgtatgaga gatttattat
     3841 ttgcgtttaa aaagtaagga ataatatggt aaaaacagca gttttgatgg caacatataa
     3901 tggtcaaggt ttcatccacg atcagctaga ttcaattaga aatcaaacgt tgagaccaga
     3961 ctatgtctta atgagggacg atggctctac tgatgatact gtaaaggtgg ttgaagacta
     4021 tataaaagag caccgtttag atggttggtc aataactagt aatgataaaa atttaggttg
     4081 gcgtttaaat tttcgtcaat tacttattga tgtgttagcc tatgaggttg actatgtctt
     4141 ttttagtgat caagatgaca catggtatca tcataaaaat aagatgcaag ttgatattat
     4201 ggaagaacgc caagatataa accttcttag cgccgatatt gatatcaaaa agttatcaga
     4261 agaggctact attcccaata attttaagtt caatggtgag gaaacaattt ctaagtatcc
     4321 attagacttt tcatatcata actatcgtca aggttggact ttttgtttac gcaagtcgtt
     4381 tgttgatcaa gtaatgactt tttacagtga taatttaatt ttgtcacatg ataatctttt
     4441 tgcaggggta tctggtgtca ttggttcagg ttacaattta aacagaagtg ttggtgaaca
     4501 taagcgacat gctgagaatg caagtgggaa tatcttgaat atgagctcaa gttacgaaag
     4561 gcatttacaa gaactgtatt ttgttttgag ttactatatc atccttgtac catttttaga
     4621 aaaaagtagc tctagtcaat taagggaagc taaagcttat ctggatttta atcagcaacg
     4681 ttataaaaat gctcaaaata aagattttgt agcaatactt aaacaaatga tgactaaaaa
     4741 acggttctat gatagctttt caaatcgcat tcgtgacttg gtgtttttat taaaagatat
     4801 aaaaaggagt taatatgaac ctcaaaaaag ttcgtctatt tgaaaagttt tttctagaca
     4861 agaacttgca taaaacatat tttatattag gcttaacgat tactattgta ttgagcctat
     4921 taatgccatt tttcaatgaa cctgatggac aataccattt agctgtgtct ggtcgtattt
     4981 caaataacat tattgatact tcttactatg gagaatataa tattgattca ggtatgagac
     5041 gacaatcgac ctcctataga gatggtacta gatttgaaaa atactattta aataaagcca
     5101 attttgttga tggtaatcat ttgcctcgtg agataaacta ttctaaacat aactttgttt
     5161 tttggggtca tcttgtgcca gcagttggct tatttttagg caaatttgtg tatccttcta
     5221 tgggggtaat gattacagta gcaaggttat ttagttcctt tgtctcaatc accttggttg
     5281 ccctcattat ccggtatgtg aaaaaaggaa agttaatcta ctttgctatt ttcctcagtc
     5341 ccgtagttct aaattcattt gctagtctat cttatgactc gactggatat attgtatttg
     5401 gagcattttt atcgcttttg ataaatcttt tagttgataa atcgttgact aaaaatcgta
     5461 gtattgctct tataggattg attcttttag tcattgttgc atgtaagcag aattactggc
     5521 tgtttttatt gctagtaata ccggtattat tgcaggttaa caatcctaaa ttaatgggct
     5581 ataatcagaa gctatcacat ttatttcaag gtcttaaatc taaaaagcta ttttggctat
     5641 gtctaacctt tttgtcactt gctatagttt atgttctgac cgcaagacat ggtggtgtcc
     5701 ttgctgtttt aagacgttat gtaatgacat ttggctataa ttatgctggt atcaatattt
     5761 taagtaatga cattacttcc tggcttgcct ctccatatcc aagtcagaat tttattccca
     5821 cttgggtatc atcagtatgg tatctcatgg tctttctagt atggttttct gaagaaaaat
     5881 ttgttaagtc aaaaaatatg ggatttcttt tcttagctag tttctttgtg ggaattcttg
     5941 gtgtcttttt tatcatgtta gaatatcgtg gagcgccaac aagctacatt gaaggagttc
     6001 aaggacgtta ctttactgtt acattgcttt tggtacaagt ttttgcatca agtattccag
     6061 cgcgtctaaa tgaggcagga aggaaagtgg tgccgtatgc gttaactggc ttggtaattg
     6121 tatcaaatgc cttattgtta tttgatactg ttattagttt aattatgagg agaaactaat
     6181 gaaacttttg aaaaatatgt tttataatac ctcataccag ttattgactt tgttgttacc
     6241 tttagtaaca gttccttatg tttcccgtgt actatctcct caaggcatcg gtattaatgc
     6301 ctatacgagc tccattgtca tgtactttac tttatttggt gctttaggga tatctttgta
     6361 cggtaatcgt gagatagctt ttgtgcagtc aaataagtat aagagaagta agatattttg
     6421 ggaattagtt gtattaaaac ttgcctctgt atctatagca acgttattat tctttggatt
     6481 tgtcttactt acaaacgaat ggcaattatt ctatttaatt caaggtatta atttattagc
     6541 gactgcaact gatatttcgt ggtattttat tggagttgaa gactttaaga taattgtcat
     6601 tcgtaatacc attgttaagc ttattacagt tgttttgacc ttcttagttg tgaagacgcc
     6661 agatgaccta gcactttata tgtttttaat tgcatttgca tcattactcg gaaatttaac
     6721 agtctggcat catttaaagc atgaaatcat caagatacca tttagtagat tagatatctt
     6781 aatacatcta agaccaactt taatgttatt tttaccccaa atcaccatgc agatttatct
     6841 ctctcttaac aagagtatgc tgggagcaat ggatagtgtg gtcagtgctg gatattttga
     6901 tcaatctgat aaaattattc gtattttatt taccattgtt tcagcaatcg gaggagtttt
     6961 cttaccaagg ctctccagtc tattttcttc aggaaaagaa aagcaagcta aggctttact
     7021 tttgaaactt gttgatttaa gcaatgctat ctcaatgtta atgattgcgg gagtggtagg
     7081 agtttcgtcg acatttgccg tcttcttttt tgggaaaggg tatgaagctg tcggtccgct
     7141 aatggctgtt gagtctttga tgatcatttg catttcttat ggtaacgctt tagggacaca
     7201 gtatctactg gcttcaagac gtacaaaagc ttacacaatg tctgcagtta ttggacttgt
     7261 ggcaaatgtt gtgcttaata tacttttaat accaatttta ggtgctatgg gagctattat
     7321 ctctacagtt attactgaat ttattgtctc cctctatcaa gctatctctt taagagatgt
     7381 atttactttt aaagaattaa caagaggaat gctgcgctat ttaattgctg ctactcttag
     7441 tggagctgta ctttattata tcaatactca aatgtcagta tcattggtaa attatgtcat
     7501 tcaaagttta gtagcagtaa ctatctatgt cggtatcgtt tttataacca aagcaccagt
     7561 cattcagtta tttagagatt ttagaaaaga gcatagaaca tgaaatattt agcgggtatt
     7621 gtcaccttta accccaatat tgaacgctta gatcagaaca ttcgagcaat ttatcctcaa
     7681 gtaagccata tctacatcgt agataatggc tcgaaaaata aggaagaaat ttctcaatta
     7741 gtggccgatt ataatgaaga ggggcaccta actgttgatt atttaacaga aaataaagga
     7801 attgcatatg ctttgaactg tatcggtcaa tttgcagtag cacaagaatt tgattggttc
     7861 ttgactttag atcaagattc ggttgtccta ggtgatttga tagataatta tgaaaattac
     7921 cttcttttac ctaaggtcgg tatgttatct tgtttgtatc aagatatgaa ccgtgaagat
     7981 ttagttatgc aagaatttga ctataaagaa attgaagagt gtattacttc ggctgcttta
     8041 atgaaaacga gcgtttttga agaaacatct ggctttgcag aagagatgtt tattgatttt
     8101 gttgactctg aaatgaatta tcgtctgtca gagatgggat ataagactta tcaggttaat
     8161 tttattggtc ttttacacga aattggacac tcttcacgag ttaaaaaatt tggccatgtt
     8221 tttcatgtat tgaatcattc accatttcga aaatattaca tgatacgcaa tgcgatttat
     8281 attataaaaa aatatggtaa gaagaagcgg cacaaatatt tagtatttat gcgaaatgaa
     8341 tttgttcgag tgttggtagc agaagagcaa aagagcaaaa aattagttgc tatgattaaa
     8401 ggcttaaaag atggcttatt gatgaaagtg gataaatatg gaaaataagg taaaaacagt
     8461 tgctgttttc tcaggatatt atctaccctt cctcggaggt attgaacgtt atacagataa
     8521 aatgacagca gatttggtta agcgtggcta ccgtgttgtt attgtaacaa ctaatcatga
     8581 tgatttaccg attatagatg aagacaaggg tagaaaaatt taccgtttac cgaccaaaaa
     8641 tattgtcaag cagcgctatc ctattattaa caaaaatcga gaatacaaca cgctgatgaa
     8701 acatgtttca gatgaaaata ttgattttgt gatttgtaat acacgttttc agttaacaac
     8761 gctagaaggg ctatcttttg caaagaagca ccgtttacct agtattgttt tagaccatgg
     8821 gtcaagtcat ttttctgtca ataatcgttt tttagatttc tttggagcta tctatgaaca
     8881 tcttctcaca gcgcgtgtta agcactatcg tccagatttt tatgcggtat ctaagagaag
     8941 cgttgagtgg ttaaaacatt ttaatataga agctaaagga gttatttata attcggtatc
     9001 tgaaagtcta ggttctgatt ttgctgacac ggcttacctt gaaaaatctg ctgatgatat
     9061 tttcatcact tatgctggta gaattattaa agaaaaaggc attgaattgc tcttagaagc
     9121 attttcgatg tcacagtact ctgaaaatgt ttatttacag attgcaggag acggacctga
     9181 attagcacat ttgaaagaga aatatcagtc caaacagatt aactttttgg ggaaattaaa
     9241 ctttgaacag actatgtctt taatggcaca gacagatatc tttgtttacc catcaatgta
     9301 tccagaggga ttaccaacct ctattttaga agctggtcta ctttcgagtg ctattattgc
     9361 aacagatcgc ggtggaacag ttgaagttat tgatagccct gagttaggaa tcatcatgga
     9421 agaaaatact cagtcactcc atgaatctct tgatttatta gtaaaagata aggcattaag
     9481 agagaaacta caacagaata ttgctaaaag aattaaagaa catttcacat gggaaaaaac
     9541 agttgaaaag ttggattata ttattcaaaa agaacttaat ctgtaataga ttatgtcact
     9601 aaattga
//
LOCUS       DATRNX010000072         9579 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRNX010000072 DATRNX010000000
VERSION     DATRNX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..344
                     /locus_tag="VBC81_001427"
     CDS             <1..344
                     /locus_tag="VBC81_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6912464.1"
                     /translation="EGETGQRIKLNDLYFFALANNTYFGGGITIWPKASALTAELDMV
                     YAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEIDGEIVELDQI
                     SLKCQKRYLYM"
     gene            354..551
                     /locus_tag="VBC81_001428"
     CDS             354..551
                     /locus_tag="VBC81_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6912465.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1157..1630
                     /gene="dfrF"
                     /locus_tag="VBC81_001429"
     CDS             1157..1630
                     /gene="dfrF"
                     /locus_tag="VBC81_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEO6912466.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1737..2267
                     /locus_tag="VBC81_001430"
     CDS             1737..2267
                     /locus_tag="VBC81_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6912467.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            2813..3055
                     /locus_tag="VBC81_001431"
     CDS             2813..3055
                     /locus_tag="VBC81_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HEO6912468.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITSHKVK"
     gene            3052..3522
                     /locus_tag="VBC81_001432"
     CDS             3052..3522
                     /locus_tag="VBC81_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEO6912469.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            3650..4864
                     /locus_tag="VBC81_001433"
     CDS             3650..4864
                     /locus_tag="VBC81_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6912470.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECISYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            5853..6062
                     /locus_tag="VBC81_001434"
     CDS             5853..6062
                     /locus_tag="VBC81_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HEO6912471.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            6174..7787
                     /locus_tag="VBC81_001435"
     CDS             6174..7787
                     /locus_tag="VBC81_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO6912472.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGKVE"
     gene            7840..7956
                     /locus_tag="VBC81_001436"
     CDS             7840..7956
                     /locus_tag="VBC81_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912473.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            8200..9315
                     /gene="ychF"
                     /locus_tag="VBC81_001437"
     CDS             8200..9315
                     /gene="ychF"
                     /locus_tag="VBC81_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6912474.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            9399..>9579
                     /locus_tag="VBC81_001438"
     CDS             9399..>9579
                     /locus_tag="VBC81_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6912475.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYF"
BASE COUNT         3413 a         1345 c         2022 g         2799 t
ORIGIN      
        1 tggaaggaga gacaggacag cggataaaac taaatgacct ttattttttc gcattagcaa
       61 ataacactta ttttggaggt ggcattacta tttggcctaa agcaagtgct ttaactgcag
      121 agttagatat ggtttacgct aaagggcata cttttctaaa acgattaagt attttattat
      181 ctttggtttt taaaaggcat actacctcta aaagtataaa acaccagact ttcaaagcta
      241 tgaccgtata ttttcctaaa aatagtttga ttgaaataga tggcgaaata gttgagttag
      301 atcagattag tttaaaatgt caaaaaaggt atttatatat gtaaggagaa aagatgtatc
      361 aagttggatc acttattgaa atgaaaaaac ctcatgcttg tgtaattaaa gaaactggta
      421 aaaaggctaa tcaatggaag gtactgagag taggagctga tattaaaatt cagtgtacta
      481 actgtcagca tgtgattatg atgagtcgtt atgattttga gcgaaaatta aaaaaagtac
      541 tgcaaccatg aaagtccttt taaacaaata attatagcta tatttgcaac caatactttc
      601 ctaaataatt gctagtatgc cgttggaaaa ttagctattc taatgttatc gaaagaagaa
      661 aggtggccat tgacaatgac gaaagttgca gaacagttgt ttatttttcg gtaagtcata
      721 tcaaaatggg aaaatggtgt ttcccaaaca aaaagatatt aagaaacagc ccatgtttac
      781 ctgcttgcaa acaaaagaat attgctacta gactaagaga tactacataa gtgtgttggt
      841 atctcttttt tgttgccgtg aaacagcaga ctatttcatt tctgcttgat taccttaaaa
      901 cttattagtc tatggtttgt caagggcttg ttgcgtgaga aatgctgaat atcacccttt
      961 acaaaacata ggctgataag taacttttca aaagcagaaa agaaatggca atgcttataa
     1021 caatatgata taatgcagat gaagacaaaa aaggaatttg tcgagctggt tagttcgaga
     1081 taattttgag tttgttaaga tgtggtgcac ttgatggaat cacaggtacg acatcatatt
     1141 atggaggatg aaatagatga taggtttgat tgttgcgagg tcaaagaata atgttatagg
     1201 caagaatggt aatataccat ggaaaataaa gggagaacaa aagcaattta gagagttaac
     1261 aacgggtaat gtggttatta tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc
     1321 taatagaatg aatattgttg tttccaccac aacagagtat caaggagata atttagtttc
     1381 agttaaatca ttagaagatg cattattatt ggctaaagga cgagatgtat acatatctgg
     1441 tggatatgga ctatttaagg aagctttgca aatagtagat aaaatgtata tcacagaagt
     1501 agatttaaat attgaagatg gagatacatt ctttccagaa tttgatatca atgattttga
     1561 agttttgata ggggaaacac ttggtgagga agtgaaatat acgagaacat tttatgtaag
     1621 gaaaaaatga attgagtaaa ttttggattt agggtgtttt cataaatata ttgcttttta
     1681 tgcatatata aattgttgta agacaaattc ctgtttgtat ttataaaaga gaaaaaatga
     1741 gaaaagtagt tttatttatt gccatgagcc ttgacggata tattgcggac ggtaatggtg
     1801 gagtagcttg gctaaatggt catggaaatg acaatgaaaa catcgacact tatacagaat
     1861 ttaccaaaga tatagatact gtcttaatgg ggtggaatac ttctcatcag gttgttactg
     1921 aactttctcc gcaagaatgg gtatataata aatttacaac ttatgtatta acacataaag
     1981 agtgcaattc ttcccaacaa attcatttta caagtgaaaa tcctgtttta ttattggaac
     2041 ggctaaaaca agaagcagga aaggatattt ggatttgtgg cggagcaaat cttgttcagc
     2101 agttggtaag caaaaatatg attgataaat attacatttc tgttattcct actctgttag
     2161 gaaatggggt tcgtcttttg ggcaacatag acagggaaat aaagttaagg ctttgtaaaa
     2221 cacagactta taacggaata accgatttga tttatatgcg tagataacta gagagaaaca
     2281 gaaaagctgt taaaccgacg gagcgatccg accacgttgg cgggaaaatc cttaagattt
     2341 ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt cggcgagctt gcgagccgtt
     2401 caaagccccc gttatctaac aaggggggca caaaaaacaa gaaacactat acactaacaa
     2461 gcggaaaacg ctgtatttat aaggcaaaac cccattttta cagtagtgat ataaattgta
     2521 cctatatcac tatgaaaatt gaatagtaaa tatgagaaag acagttgatt tcaaaaaata
     2581 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
     2641 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
     2701 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
     2761 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
     2821 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
     2881 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
     2941 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
     3001 tataacggta aatgcgtaca gttataccgt tatcacttct cacaaggtaa aatgataaat
     3061 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
     3121 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
     3181 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
     3241 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
     3301 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
     3361 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
     3421 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
     3481 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
     3541 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     3601 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     3661 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     3721 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     3781 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     3841 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     3901 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     3961 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     4021 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     4081 actggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     4141 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     4201 aaaggaaaaa tacaaggctg gcgaatgtat ctcctacttc cgtatgcaga aagattacag
     4261 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     4321 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     4381 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     4441 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     4501 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     4561 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     4621 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     4681 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     4741 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     4801 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     4861 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     4921 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     4981 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     5041 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     5101 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     5161 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     5221 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     5281 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     5341 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     5401 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     5461 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     5521 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     5581 ttctttgaca actgaataaa gtaattcaat acgtttgaca gacagctagc cgttgaaaca
     5641 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     5701 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     5761 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     5821 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     5881 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     5941 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     6001 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     6061 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     6121 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     6181 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     6241 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     6301 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     6361 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     6421 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     6481 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     6541 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     6601 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     6661 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     6721 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     6781 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     6841 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     6901 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     6961 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     7021 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     7081 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     7141 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     7201 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     7261 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     7321 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     7381 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     7441 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     7501 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     7561 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     7621 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     7681 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     7741 tacaggaaat tgagatatat taccgattta ttggaaaagt tgagtaatca acaagagtaa
     7801 tatttttaac taagggaaac cggggatacc acaccagatt tggataattt aatcaggtta
     7861 gctgagattt ttgacgtgag cttagatgaa catgttttag ctaaaacaaa tgaagttaaa
     7921 gttgaacgta tttatgaaaa aaatcttgat ctgtaaaaat ataataaaat ttattggttt
     7981 atttttcgaa atattattcg atctctacta attatcttag ctatattaac tttcttataa
     8041 gctctaggtc ttccctttgt ttcggagtgg ataatttaaa tgaaggtgaa agagtggcat
     8101 aatgataagc aaatctgaaa ttttcttctt atcataccgc tttttcttct attttttgat
     8161 ataatggtct tgattgaaat ttgaatggag actcactaaa tggctttaac agcaggtatt
     8221 gtaggcttac caaatgttgg taaatcaact ttatttaacg caattacaaa agcaggagca
     8281 gaagctgcta actatccttt tgcgactatt gatcccaatg ttggtatggt agaggtacca
     8341 gatgagcgtc tgcaaaaatt gacagaattg attacgccta aaaaaacagt tccgacaacc
     8401 tttgaattta ctgatattgc aggtattgtt aaaggtgctt cgaaggggga aggtctagga
     8461 aataaatttt tagctaatat tcgtgaagtg gatgctattg ttcatgtggt acgtgctttt
     8521 gatgatgaaa atgttatgcg tgaacaaggt cgtgaggatg ccttcgttga tcctatagca
     8581 gatattgata ctattaatct tgaattaatc ttagctgatt tagagtcaat caataaacgt
     8641 tatgcgcgtg ttgaaaaaat ggcacgaact caaaaagata aagaatcagt agcagagttc
     8701 aatgttttac aaaagattaa acctgttttg gaagatggga aatcagctcg taccattgag
     8761 tttacagaag aggaagcaaa agttgttaaa ggtctctttt tattaacaac taaacctgtt
     8821 ttatatgttg ctaatgttga tgaggacaaa gtagctgatc ctgatgatat cgactatgtt
     8881 aatcaaattc gtgcatttgc agaaacagag agtgcagaag tagttgtgat ttctgctcga
     8941 gctgaagaag aaatatctga acttgatgat gaagataagt tagaattttt agaagctatt
     9001 ggattaacag aatctggtgt tgataaatta acacgagctg cttatcatct tttaggcctt
     9061 ggaacttact ttactgctgg tgaaaaagag gttcgcgctt ggacatttaa gcgtggtata
     9121 aaagctccac aagctgcggg tattatccat tcagattttg aaagaggttt tattcgtgca
     9181 gtaacgatgt catatgatga ccttattcaa tatggatctg aaaaagcagt aaaagaagct
     9241 ggccggttac gtgaagaagg aaaagagtat attgttcaag atggcgatat catggaattc
     9301 cgtttcaatg tctagtaata aataatcaat ttgttaactt ttaggttaag gatattgtta
     9361 ttatccttaa ccatttttgc gttttaatgg agaagataat ggtaaaaatg attgttggat
     9421 taggaaatcc aggaagtaaa tataatgata caaaacataa tattggattt atggctgttg
     9481 acagaattgt aaaaaaactt gacgtcaact ttacagaaga taaaaatttt aaagcagaga
     9541 taggtagtga ttttatcaac ggtgaaaaaa tttatttta
//
LOCUS       DATRNX010000073         9526 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRNX010000073 DATRNX010000000
VERSION     DATRNX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1204
                     /locus_tag="VBC81_001439"
     CDS             <1..1204
                     /locus_tag="VBC81_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6912476.1"
                     /translation="EKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEWV
                     RNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDII
                     VNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTAE
                     ELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNII
                     HFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLFT
                     LTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKLI
                     TLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVLI
                     NELNLNQL"
     gene            complement(1251..1790)
                     /gene="nusG"
                     /locus_tag="VBC81_001440"
     CDS             complement(1251..1790)
                     /gene="nusG"
                     /locus_tag="VBC81_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6912477.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(1996..3720)
                     /locus_tag="VBC81_001441"
     CDS             complement(1996..3720)
                     /locus_tag="VBC81_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO6912478.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYS
                     FRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYE
                     ANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQN
                     LTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSV
                     QKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVD
                     TEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVA
                     KPVAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFT
                     AGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAK
                     PEAKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLIT
                     IVLKHKKN"
     gene            complement(4034..4207)
                     /gene="secE"
                     /locus_tag="VBC81_001442"
     CDS             complement(4034..4207)
                     /gene="secE"
                     /locus_tag="VBC81_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6912479.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(4243..4395)
                     /gene="rpmG"
                     /locus_tag="VBC81_001443"
     CDS             complement(4243..4395)
                     /gene="rpmG"
                     /locus_tag="VBC81_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6912480.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(4444..6765)
                     /locus_tag="VBC81_001444"
     CDS             complement(4444..6765)
                     /locus_tag="VBC81_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6912481.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            6809..7693
                     /locus_tag="VBC81_001445"
     CDS             6809..7693
                     /locus_tag="VBC81_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6912482.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            complement(7718..9358)
                     /locus_tag="VBC81_001446"
     CDS             complement(7718..9358)
                     /locus_tag="VBC81_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912483.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
BASE COUNT         2930 a         1792 c         1453 g         3351 t
ORIGIN      
        1 ggaaaaagca gttgcacttg cagttgattc aaactacttg gataaagcct tagtaacaat
       61 aaagtctatt tgtgtttata atagaaatat aactttttat ttattcaatc aagatacccc
      121 agttgaatgg gtacgtaata taaacaggaa actagagcct ctaggatcaa aactgattaa
      181 tgttaaaata tataactatg atattgctca tctaacgact tttctaactg ttagtacatg
      241 gtttagatta tttttagcag attatatacc tagttcacgt gtactttatt tagattcaga
      301 tattatcgtt aacactaatc ttgattactt atttgaacta gattttaaag gtcattactt
      361 agcagccgtc aaagatcccc ataaaaatga agaaggaggg tttaatgctg gcatgctttt
      421 agctaatcta gaactatggc gggaagatgg cctcactaaa acattactaa aaacagctga
      481 agaactccac cgagttgtca aaacagggga tcaaagtatc ttgaacattg tttgccataa
      541 tcgttggtta tctctgaaca aaacatggaa ctttcaaact tatgatgtcg ttagccgcta
      601 taatcatcga tcttatttat atctaaacat agaaaataga actcctaata ttatacattt
      661 tttaactagt gacaaacctt ggaatgaaaa tagcgttgca aggtttagag aactatggtg
      721 gtattacttc caacttgatt tttgccaatt aaccggcaag caaagaaaag tgatttctta
      781 cgaaaagtcc atggaattgc tttctgtttc agatattcat cttttcaccc ttacatcttc
      841 cgataatttg gaacacattg aatcgctaat ttgtagatgt cctactgttc aattccatat
      901 tggtgcctac acaacagtgt caaataaact tagcaaacta gaacaatatc caaatgtcct
      961 agtttaccct gaattaattg aggcaagaat tgaaaaatta ataacattag ccactgcata
     1021 ccttgatatt aatcatggtc cagaagtagg taatatttta caaagagttc atttgaaaca
     1081 aaagccaatt tattctttca acaacacgtc tcatcaagag aatataacca aacatatcgt
     1141 taatcataat tctattgata atatggttgt tttaattaac gaattaaacc taaatcaatt
     1201 ataaattaac aaaaatccat ctttatttta agatggattt ttcagaagtt ttataattct
     1261 gcaacttggt acaactcaag ttctgcttgt gtttctgagc cgaacatgtt gatcattaat
     1321 ttaactttgt tattctcaat ctcaactaca cgtccttctt gtccgatgaa agcaccatca
     1381 atgatttgaa caacatcacc ttccttgata ttagtatcaa agacatcaac ggtttgtccc
     1441 attgagatga gtattgaacg aatctcttct tctaacaacg gtgttggttt agagcgattg
     1501 ccgtgtgatc caacaaatcc agtaacgttt ggagtattac gaacaacaaa ccaggcttca
     1561 tcagtcatta ccatttccac aagtacataa cctgggaagc gattttcttc aatttctttt
     1621 gttttcccat ttttctcaac atttacagtt tgagtaggga tttccacacg caaaatgttg
     1681 tctaacatat tataagtttg cgcacgttgc aagagatttt cctttacttt attttcataa
     1741 cctgagtagg tttggagtac aaaccaaccc ttatcaaaag aatctaacat aagttttcct
     1801 ttccttaata aaaaagcctt ttacggctta ttggactgct attttcaata tatattataa
     1861 agaaaataaa ataagctgtc aattaaagta ccgactaaat cattctaaaa ttcaaataaa
     1921 aaaggcaaag ttctgatgag gtttgcctta agattttgaa aggatagacc tttcgattta
     1981 tgtttaaaaa taaatttaat tttttttatg ctttaaaact atcgtaataa gacctgcact
     2041 taacataaca attagtgata caattgctaa gagtggactt gcggcttcac ccgttgatgg
     2101 taatttttta ctatactttt tgttttcaat agctttttta gccaccaagt ttccgctagt
     2161 attaaccgat tttttggttg ctggcttggc ttctggttta gcctctggct taacgtctga
     2221 tttaacttct ggcttagcct ctggtttggc ctctggctta acgtctggtt taacttctgg
     2281 cttagcctct ggtttggcct ctggcttaac gtctggttta acttctggtt taactttttc
     2341 gtaaacatat gtacgtactt gtgctcctgc tgtgaagaca tttgaaacag caccttccgt
     2401 tctaacaagt ttgtaacccg ctacttcttt cgctgaagtg acaaaagctg cgccttcttc
     2461 tccttcgaag tgacgcgact tagctacttc ttttccttct gtgtctacat acttaacatc
     2521 gtatgtacct ttagctactg gtttggctac tttttcgtaa acatatgtac gtacttgtgc
     2581 tcctgctgtg aagacatttg aaacagcgcc ttccgttcta acaagtttgt aacccgctac
     2641 ttctttcgct gaagtgacaa aagctgcgcc ttcttctcct tcgaagtgac gtgacttagc
     2701 tacttctttt ccttctgtgt ctacatactt aacatcgtat gtacctttag ctactggttt
     2761 ggctactttt ttatctttta attctaaact ttgtaaagca tcacgtagct tgttagctaa
     2821 ttgatttgct tgatcagtgg taataaattt tccctcttcc acatgatcat aagcttggtc
     2881 aacagctttt tgaactgaaa gtatatctac gtatttgtcc tttacttgat catctttaag
     2941 cagatcttta gcttgattat accatgtagt caatcttgtc aaggcttctg gtgacaaagc
     3001 tggttttgca gcttctgtag gtgcttgttt aggtacttga atatttggtt tgttattttt
     3061 tgttaaattt tgtagctcaa gtaataattt tttaacatct tctaacaatt tttgagctgc
     3121 attaaatcct tcctgaccat tccaaatatt aaattttcct ggacttcgtc tttcctcaaa
     3181 actgttcaca taagttccaa cagaatcttt gtaacgatga ggtaatatag atttatttgc
     3241 ttcataaatt tctttagcct caagagctat cgcaagacct gtagtactga tattatcaat
     3301 tttactacgg tagtcagcag aatttgtatc taactgtttc cttgcaactg cgttataaaa
     3361 ttcatttaat agacgtttaa cgtcgttcac atcattagat gtattaattc taaaactata
     3421 gtcactagct agtttgtcta cttccgaatt agttatatta ggatgtgaaa ttattgaaga
     3481 aagtgcttgc tctttagcac cttttaattc gttcggtatt gatatgggtg tgagttcatg
     3541 aactgatttc acatctttca atttgcctaa tttttcgact tttaatgtat caggactatc
     3601 agcagacact gtaggagtat ttacaatacc actacatact ataaacgccg ctgacattga
     3661 agccaaacca taagctgttt ttcttaaaaa gtattttact tttttttcgt tattattcat
     3721 attcgctccc ttatatagtt agttaaaaat cctaatttat taggatagga ccacgtttat
     3781 tttaatttaa tttgagttcc ttaactaatt atataacttg gagtttttta tttcaacact
     3841 ttttctttaa tttatttaca ttttattaaa ttgaataact tttattttaa tattatttgc
     3901 ttaataatct agtaaaaaca atcaaaagat tattttttta taatttaatc ggatatatat
     3961 gttattttcc acacaaaaaa acccttagta aaatctaagg gtttgggttg tttctatcta
     4021 aaataatcta ttattaaaat cgattaatca agtccatgac tcctgcagct aacagtttgt
     4081 caaagatgta gataacgatt gtgaaaaata cagtgtattc caagatggaa ataaaatctt
     4141 tccaacgttg ttttcgattt ggccaagtag tgtcacgcaa tacttgaaaa atccctttga
     4201 caaacattat cagttctcct ttttgcagtt tgttacgcta tatcaacgcg tttctttgtg
     4261 tattgtatat ttcttacaat atttacaaaa tttatttact tctagtcgtg ttggcttggg
     4321 ggtgctactt actccgattg agtaatttcg gcttccgcat tctgtacacg ctaggcttgc
     4381 tttcttttgt gccataacgc ctccataagt gttattttaa catgatttgt ataatcctgc
     4441 aagctatcta aagtagtcct ttagactatt ccaagcatct tttaccttct cttggaaatt
     4501 tgaatcttcc acagctctag aaatatcttg tgcagcctta cttgccgaat cttttaaatc
     4561 attaataatt gagcgtgatt cttggttcgt tgtattacta gcatcttgtg cattataaac
     4621 tgaaccaata ccgttttgga aataagcatt ctcaatatga gtaaaactag accccttagt
     4681 atacggtagt atatatgaag cttgcgtgct aaatatattt gaagctgtac ctgcactaga
     4741 atctgtcaaa taatggtgtt tatctgtgtt cttaaagcca acccattgac taataacgac
     4801 atcaggcgta tagccgacta cccactggtc tccagataaa ttggggttaa aatctgtttc
     4861 ggttgttcca gttttacctg ccattgtata accataaaca ttggcattaa tagctgtacc
     4921 gtttgagaat gttcctaaca tcatactagt cattttacta gccactgagc gactaataac
     4981 tcgcttaggc ttatctgtaa attgctttac taattttcca ttagctgtct caattcgagt
     5041 aatcaaatga gcgcgatgca ttataccatc atttgcaaaa gttgagtaag cttgtgccat
     5101 ttctaaagga ttagtagtta cacttccacc tagcgccact cctagctctt tgtttgctga
     5161 tgacatattt agaccaaact tcctgccata agtaaaagct ttattgattc ctagtttatc
     5221 taaagtataa acagcaggaa tattataaga attagctagt gcttgataca tcgggatact
     5281 ttcagtctca attccaccat agttacttgg tttataacca tgaaaatctt gaactttatt
     5341 aggaagttct ttatctattg accatcctga agcaatagct ggtgaataaa caactaaagg
     5401 ttttatggtt gaggctggac ttctctttga ttgagtcgcg tagttaaaac ttctaaactg
     5461 tgcatcttta gtactttgaa cacgtccaac taaacctctt acaccaccag tttttggatc
     5521 tagagctacg ctagcacctt gggcagattg accatcatag tcagatacag ggaagaggct
     5581 agtatcatca aatgtttttt gcattcctga ttgataattt tgatctaatg cagtgtaaat
     5641 cttatagcca ttattgacaa tatctttttc tgaaatacca taagtatcaa tggcttcatt
     5701 gacaacagca tcaaaatagg atgggtactt gtaatcatta attttaccag cataggtatc
     5761 tgctaatcta tttttcatac caattgaagc agcttcttta gcttgagact tggttagttg
     5821 acctgcatca accatcgctg ccaaaacagt atctcttcgg ttagttgcat tttcaacaga
     5881 ataataagga ttataaactt ctggtccttt aagcattcca gcaagtgtag cagcctcatc
     5941 tactgtcaaa ttagcagcac tggttccaaa atatttccta ctagcatctt ccacacccca
     6001 aacaccatta ccaaaataag agttattaag atacatggtc agaatctcag ccttgctgta
     6061 tttcttagtc aattctagcg ctaaaaagaa ttcacgcgcc ttgcgtttaa tcgtttggtc
     6121 ctgtgacaag taagcatttt ttgctaattg ctgggtaata gttgagcctc cgccaaattt
     6181 cccaagtgta gctactgcca aaaaaaatcg cttaaagttt acaccattat tttcataaaa
     6241 agttctatct tcagttgcta taacagcatt tttgagatga tcagaaatag catttaactc
     6301 tacataagtt cccttttgtc ccgaaagttt tccagcgtat tctttattct tatcataaat
     6361 ggtggtggtt gtttttagag cactctgtaa atcactaact gtagcagttt tagaaagata
     6421 aaaaagatag ctaccaaata taagaataaa agccattaag gcgataaaaa gcaacttccc
     6481 aatacgatag cgccgccaaa aacgacggat aggattctta ggagaaggga ggtaacgatc
     6541 aactttttgt agccattcaa gtcgagattt agcattttcc ttttggtgac gaatgcctcg
     6601 ctgatactgg gatttactag gtcgggtaat atcatcaata tcatagactt cttcgccatc
     6661 caaattgcta gtttcttctc tagtttcttg ttcatttttt cttgtcattt cgtcgttgtc
     6721 ttcatcaaca cgaaataagt ctataaactt atcaaataat ttcatagact tattatatca
     6781 attttcaata aaatgctata ataaaaccat gtcattttca ttaaaaatta gaaatccata
     6841 cggtgaacat accgttaaag aactccttga agattatttt ttgattccac gtaagattag
     6901 acattttttg cgtgttaaaa aacatgtact tataaacaat gaattcatta attggcaaac
     6961 tgtcgtccaa gaaaacgata ctattacctt aatctttgat gatgaggatt accctactaa
     7021 aaaaattcct ctgggcagag cagagcttat tgattgtctt tatgaggatg aacatcttat
     7081 tatcgttaat aaacctgaag gtatgaaaac tcacggtaac caaccaaatg aaatagcact
     7141 gttaaatcat gtatctgcct attctggaca aacatgctat gttgttcatc gcctagatat
     7201 ggagaccagt ggagctgttt tatttgctaa aaatccattt atacttcccc ttatcaatca
     7261 acgcttagaa cgaaaagaaa tttggcgtga atattgggct ttagttgaag gaaaattttc
     7321 acctaagcat caagttttga gagacaaaat tggacggaac cgtcatgaca gacgtaaacg
     7381 aatcattgat tctaaaaacg gtcaacatgc tatgacaatc attgacgttt tgaagtatat
     7441 ccaaaatagt agtctcataa aatgccgact ggaaaccgga agaacccatc aaattcgcat
     7501 tcacttatct catcacggac atcctttaat aggagatccc ctctacaacc cttcttctaa
     7561 taatgaaagg ttaatgctac acgctcaccg attgactcta tcccatccat taacttgcga
     7621 aactattagc gtagaggccc cttcatctac tttcgagaag gttttaaaca attataaaaa
     7681 aggagttgga taataattat ccaactcctt ttaatcatta ttgcttagtt aatggcttac
     7741 tgagtagtgg tttatggtaa tcaaattctc catcactaga tgtctgccag attactggga
     7801 tgatcttatt cccttcaatt tttattccat aggcgtattt aactttaaga ccaccgatac
     7861 ctcctgttac aaaagttgta gtatcagtac ctgattcata ttgataaagg tagagcccat
     7921 ttcccttatc ctccaacttc gtaactttag cagaatggtt actttcctta gatttactat
     7981 ccttgtaaac ttttttctga gtaactttac catccttatc aaaagtcatc gttactgtac
     8041 cgtcctcaac ttcacctttc caacttccta caaggttctc tggaaaaata gaaacgggat
     8101 cagccttaac tttattagtt tccttagcta ctgtgatatc ttttttacca tcattagaaa
     8161 taaggtaact atttcccgat tttttcataa taaaattacc agttaaattc tgaataatgt
     8221 ggccatatgt tttcttatca ggatctgttg atttatcata attaaaatca taatcggcag
     8281 caaaattaac ttgataactt tctttcccaa cctgtgtcat tttattaagg acaatattag
     8341 gaatagtgaa atggctcgct ttacgattat cagttacaaa ttttgcttta atactttctt
     8401 tcaatccttt gtaaaaatca ttttcggcac ctttttcaaa aactgttcct aaagtctgag
     8461 ggtcttgccc agttgataca taaagtatca attgattgaa agcagttatc aatttctgtc
     8521 cagcttcttt ttcatttaat aaaccatcaa catctaattt gattgtctga ttatcagcga
     8581 tagagattaa tttttgttta tgagaggtta tctcaccatc attaaaaact tttttgatat
     8641 aagcttttga accatcagtc actggataat tctctacgga atagctacca tcttttaatt
     8701 tagcaatacg attatcacca aaataaagat taccatccat aagattactt gttactgtaa
     8761 aagatttaaa agcgaccgat aaatcaatag tttggttttt accatcataa tcttttttga
     8821 gtttaatctc ttttccgtca ctagtccctt caaggctagc agtataatgg gttctaggta
     8881 aacgttccac cgttactgaa aaatgatctg aattgcttgt cgcgaccttt ttttgattca
     8941 acaaaacatc caatttagga acattggttt tcaaggtcaa agacataggc ttattagcaa
     9001 tacgataatc agggaatata cctaagcgac tacctacacg ctttagatac attgaatcat
     9061 ttttgtttcc ttcttttatt tcctgcccta atttacgcgc ctcactttta gacaaaggct
     9121 caaaattagc gtaattggcc tcatcatttg tgatagattc tcccgtatct gaccaaacca
     9181 tgtattcttt aatattatca aacgcttgac ctgacgtacg agaacgtgct gctacaaaac
     9241 gactaagtac ttgtcccttt gaaaaataaa agaatcctag actcagaagt gatactaaaa
     9301 ctataccaaa aataactaag cacactttaa caactttttt cttaaaaaaa tcaaacatac
     9361 cttatcctct cattcacttt atattaaagt aaattgctta aagaaaagtt acaacactga
     9421 taaaacagct gtatttgtac ttgtaaccgc tatttttcta ttataacaaa acgaaaaaag
     9481 gattgaaacc ataatccgat ttcaacctct aaaatttttg tctatg
//
LOCUS       DATRNX010000074         9384 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRNX010000074 DATRNX010000000
VERSION     DATRNX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(50..1369)
                     /locus_tag="VBC81_001447"
     CDS             complement(50..1369)
                     /locus_tag="VBC81_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6912484.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            complement(1523..2020)
                     /locus_tag="VBC81_001448"
     CDS             complement(1523..2020)
                     /locus_tag="VBC81_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6912485.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            complement(2156..3520)
                     /gene="radA"
                     /locus_tag="VBC81_001449"
     CDS             complement(2156..3520)
                     /gene="radA"
                     /locus_tag="VBC81_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6912486.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            complement(3682..4128)
                     /locus_tag="VBC81_001450"
     CDS             complement(3682..4128)
                     /locus_tag="VBC81_001450"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6912487.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            complement(4191..4276)
                     /locus_tag="VBC81_001451"
     tRNA            complement(4191..4276)
                     /locus_tag="VBC81_001451"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4240..4242),aa:Leu,seq:aag)
     gene            complement(4323..5249)
                     /locus_tag="VBC81_001452"
     CDS             complement(4323..5249)
                     /locus_tag="VBC81_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912488.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            complement(5358..6962)
                     /locus_tag="VBC81_001453"
     CDS             complement(5358..6962)
                     /locus_tag="VBC81_001453"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6912489.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            complement(7235..7810)
                     /locus_tag="VBC81_001454"
     CDS             complement(7235..7810)
                     /locus_tag="VBC81_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6912490.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEV"
     gene            complement(7999..9282)
                     /locus_tag="VBC81_001455"
     CDS             complement(7999..9282)
                     /locus_tag="VBC81_001455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6912491.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
BASE COUNT         2870 a         1987 c         1436 g         3091 t
ORIGIN      
        1 tttcgataaa aaaagtaaag caaaattgcc ttacttttta aaaatattct taaaagttaa
       61 acaaatcatt aaaattctct accattgttg ggtgagtgaa aatttgtttt tggaaataag
      121 tatatgggat tttattatcc attgccattg tgatgatatt aattaattca tgggattctg
      181 caccaaataa gcgagctccc aaaataagat ttgtctcagt atctaccaca actttaaaaa
      241 tacctctaag gtcattatta acatgtgcac gtggcatggc agaaacaaga aggctattag
      301 atttaacttg atatcctttt tccttagcgg tcttttcatc tagacctacc gttgctaaag
      361 gaggatttgt aaaggttgaa gtcggtactg ctcctcgatt ttttagagaa taatccttat
      421 caccattcaa ataatttaag acaatacgtg agtcatctaa agaaatatat gtgaattgag
      481 ggccgccatt aacatcacca actgcaaaaa tattctcaac agaagtttgg caatattcat
      541 caacagcaat agctccacgt tctgtcaatt taatatctgt attttcaagt gctaaacctt
      601 ctgtatttgg cttacgtcct gttgcatata atactgcatc aaagcttaat ttttcatcct
      661 caaagctaat aacaacgttt tcatcttcat tttgcacaga tttgatatcg gctgataatt
      721 taaaagatat ccccatctct tctaaataat cttgagccat ctctgataat tcctcttcct
      781 cacgcgcaaa gatacgagat tgactatcaa taactgtcac tttgctccct aattcggaat
      841 ataaagtcgc aaattcaagc ccaatattac cgcctccaat gatacctaac cgtttaggaa
      901 gatgagccaa ttcttggatt gccgttgaat cataaacatg ttgagaatca gctaatcctg
      961 gaattggtaa ttgtactgac ttagcaccag tattgataat aataacatca gctgtaagtt
     1021 cttgtttgtc agctcctcct gttaattcga ccactttatt tgatataaag cgagctttag
     1081 cattataaac atccacagtg tctttattat ccaacatagc aaaattttta gcacgtaaac
     1141 gacttgtaac ttcatttcta gtagtcatag cttcttgaaa atcgtgattt ttgctagctg
     1201 atactaaaag cgtttttgtc ggaatacatc caatgttgat acatgttccc ccatacattt
     1261 tatcgtcttc ttcgaccatt gcaacgcttt taccttgtgt tgctaatttt gctgctaatg
     1321 ttttacctgc tttaccaaaa ccaagaacaa tgacatcata ttttttcatt ttaaatttcc
     1381 tccgctttta taatatagtt tcagtatagc aaaagatgtt tcctctgtct gaattccagc
     1441 ataaggcatg ataagcctaa ctgttacctt taaatctagt tttctaaaat aaaaaaaccc
     1501 atcgctaagg acaggatttt ttctatttaa cttccaccat acgaccagta tctacatcat
     1561 aaatagcacc agaaataaca acatcatttg ggattaatgg agaagcatgt aattttgcca
     1621 catcttcacg aacgctttct tcaatatcat taaaaggaag aaaatcatgt ccatgcatat
     1681 caacacccaa atctctctgt aattgagcag caaaagcttc gttagtaaac gtctgtgctc
     1741 cacaatctgt atggtgtaga acaacgattt ctcgtgttcc taattgttgt tgcgaaataa
     1801 ctaaagaacg tagaacatcg gctgtaactc gtccaccagc attacgtaaa atatgagcat
     1861 ctcctaaagc tagccctaga gcttgagcaa cgtgtaaacg agaatccata caggttacaa
     1921 tagcaacctt tgtctttggc ttgataggta gatgagctgt tccatgtaag tcagcatagg
     1981 cttgattggt ttttaaaaag ttatcaaaat aagtggtcat agagatctcc tttgttagtg
     2041 taacatcatt cgatatttac taaataccaa cccttttata ttatcatttt gcccattatt
     2101 tttcacgaaa tgtctctcgc caccaaaaaa gattcggctg aaccgaatct aatcattatg
     2161 ttgaaaacac tgcttttaag acctgactaa cagttgtcac accaataaca tcaatgcctt
     2221 taggtatttc aatcccagct agagaattct ttggtgcgta aattttagta aagcccaatt
     2281 tactagcctc attaatacgt tgctcaatac gtgtcacgcg acgaatctca ccagtgagtc
     2341 caatttctcc aataaatgat tcttgtggat ttgtgggttt ttctttgtaa ctggaagcaa
     2401 tagctacagc aacagctaaa tcaattgctg gttcatccaa tttaacacca cccgctgatt
     2461 ttaaataggc atcttgattt tgaagcaata aaccacaacg tttttctaga actgccataa
     2521 taagactgac acgattaaag tcaagacctg tggttgtgcg cttagcattt ccaaaaacag
     2581 ttggagtcac taaagcttga acctctgcta aaataggcct tgttccttcc atagtaacaa
     2641 caatagcaga tcctgttgca ccgtctaaac gttcttcaag aaaaacttga cttggattca
     2701 aaacttcaac tagaccaccc gactgcattt caaagatacc tatttcattg gtcgaaccaa
     2761 aacggttttt gactgcccgt aaaatacgga acgtgtgatg acgctcccct tcaaaataaa
     2821 gaaccgtatc caccatatgt tctagcattc gagggcctgc taatgttccc tccttagtca
     2881 catgacctac aataaaagta gcaatattat tagttttagc taactgcatc aactcagcgg
     2941 tcacttcgcg aacttggcta acagagcctt ggacacttga tacttcggga ctcataatag
     3001 tttgtataga atcaataata agaaaatctg gtttaatctt ctcaatctcc gaccgaatac
     3061 tttgcatatt agtctcggca taaaggtaaa attcattatc gatatcgcct agacgctcac
     3121 ttcgtaactt aatctgttct gcagattcct cacctgagac atataatact gtacctttat
     3181 tagctagctg agttgatact tgtagtaata aagtagattt acctatccca gggtctcctc
     3241 caatcagaac caaacttcct ggtacaacac cgcctcctag aacacggtta aattcatcca
     3301 tatcagtctt agttctagaa tagttaattg aggaaacatc ttttaacttc gtcggtcggg
     3361 acttttcacc gttcaaacta actcgtgcat ttttaacttc ttgaacttca acctcttcta
     3421 caaatgaaga ccaggctgag caatttggac aacgtcccaa atacttaggt gattggtaac
     3481 cacactcttg acaggtgaag actgattttt tcttagccac tcaggcctcc tttataactt
     3541 ctaaattaat atgatagctt ggtaccaaat aactagtaca ctcattatat cacaccaagt
     3601 tagagaaaat ataagtttac tctcatcaaa aaaggctaag aagtaagtct agccttttct
     3661 aacattattc gtaaaataat tttattttcc agtactacca aaaccacctg tacgcattcc
     3721 tgtagcctga tcgtcatctg ccaataagaa tggggcaaaa acagcctgaa caatccgttc
     3781 cccttcttct aaaataacag cctgatcagt aatattttgc atctgtgcaa aaatatgtcc
     3841 ttcattgact tggttattat agtagtcacc atcaataaca ccaactgaat ttattaggac
     3901 aatccctttt ttacgtggat tcgatgaacg atcataaagg tacaggacct cccctggttg
     3961 catgtgtgct ttaataccgg tcgggaccaa tgtaatttca ccaggttcaa taaccgtttt
     4021 tttagctact tttaaatcat agccagctgc gtgagctgtt tctcgttttg gtaataactc
     4081 tttattgcta aattggctaa ctaattcaaa gccacgtact tttgacatgt gatactccat
     4141 ctttatctta aatatgcaaa aagtcgagtt ccaaatgaac tcgactagtc tgcggagaga
     4201 gggacttgaa ccctcacgac caaaagcggt cacaagatcc ttagtcttgc gcgtctgcca
     4261 attccgccat ccccgcttaa caacataata aattatatca acttatcata ttgttgtcaa
     4321 tattattttt catatttttt caaaaaacta gagatacgtt tctcatattt ttctggctct
     4381 gtttcaaaag atttcgcatg ttttgcccct tttacaatat aaagctcttt cttacctgct
     4441 gtagctttat agttgtcata aaccatactt gttggaacaa aattatcctt atcaccatga
     4501 ataaagaggg ctggtaaatt attctttttc aattgttcga cactacttgc ttgtccatac
     4561 gaaaaacctg ctctgatttt agaaattgtt gaaacttcat ataagagtgg gaaggctggt
     4621 aaaccataca tctctttagc ctgaaatttt aattcatccc aaacactaga ataaccacaa
     4681 tcttcaatga tattaacaac ctgactaggt aatttttcac cactagccat catgactgtt
     4741 gctccaccca ttgaaacacc aaataaagta atttggcttg atggattctt atccactatc
     4801 atttctgtcc atttgataat gttctcgcgg tcgttccagc catagcctat caactgccca
     4861 tgactttcac catgtgcaat gttgtcaggc ataagaacat tgtatcctaa cttatgaaac
     4921 agccaaccat atgccttcat attctctttg ctattcgcaa aaccatgaac gacaacaact
     4981 gtcttatgag ttttcttagc agcaggaaca taccaagcaa cttgctttat attttggttt
     5041 gtcatttcta ttttttgctt taatagctta tcaaaggatt tatcataagc atataaagag
     5101 tttccaggct tacgttgacc atttgaaata aaggatttat catctcgaac ttgggcaacg
     5161 tggaaaaaat aaaaactagc tgctacacta attaaaaaca aaataacaac aatcatttta
     5221 ataaactttg ataatcgaat ctttttcatg caacctatta taccttattt ttagaaaaag
     5281 aaaagagcta atgatacttt gatagtgaaa ctatctaaaa aaataaaatc gcctcctaat
     5341 actgggaggc gataaagtta tttcatattt tcaactgctg ctgttacaaa agctgtgtaa
     5401 agctcttcag catggttagg acgtgattgt agttctggat ggtattgtgc tgcaacaaag
     5461 aattttttct ctggtaactc aacaacttcc atcaaacgat tatccggtga tacccctgag
     5521 aagacaaatc cagcagcctc aaattgctca cggaattttg tattaaattc ataacggtga
     5581 cggtgacgac gttggacaac ttcttgatta ttataagctg ccgcagctct tgaacctgct
     5641 ttcaatttac atggatatag accaaggcga agtgtaccac ccatatcttc aatatcgatt
     5701 tgatcacgca tgatatcaat gattgggaac ggtgttttag ggtcaagctc tgctgaatta
     5761 gctccatgaa ggtttaaaac attacgcgca aattcgacac aagttagctg cattccgaga
     5821 caaacaccaa gcattggaac atcattttca cgtgcatagc gaattgcttc aattttaccc
     5881 tcactacctc tttggccaaa tccaccagga acgatgatac catctgcatc tccgacaagt
     5941 tctttaatat tatcttctgt cacttcagca gcatttaccc attttaggtc aatagcaaca
     6001 tcgttgacgt accctgagtg cttaagggcc tccacaactg aaaggtaagc atcaggtaat
     6061 tcaacatatt taccaaccaa tgcaatttta acttttttct caaggttcat tactttatca
     6121 accattgctg accattctgt catatcagca gcaggtgtct caagcttcaa gtgatcacaa
     6181 acaatttgat ccatattttg agcttgcatg ttaagtggaa tttgataaat atggtcaaca
     6241 tcaagggact ctataacagc ctctggtgca acatcacaaa attgtgccaa tttattctta
     6301 atactttgac ctgctggttg ctctgtacgg ataacaagca tattaggttg gatacctagg
     6361 ccacgtaact ctttaacaga atgttgagtc ggttttgttt tcatttcgcc ggcagccttt
     6421 aggtatggta ataacgttgt atgaatgtac ataacattat cagaaccaac atctgccttc
     6481 atttgacgaa gtgcttctaa aaacggaagg ctctcaatat ctccaacagt accgccaact
     6541 tcagtaataa taacatctga atctgtagtt gtcgcagcac gtttgatttt ttctttcaaa
     6601 gcatccgtaa cgtgtgggat aacttgaaca gttgcaccaa ggtattctcc acggcgttct
     6661 tttttaagaa cttcactata aatcttaccc gttgtaacat ttgaatactt gtttaagtta
     6721 atatcaataa aacgctcgta gtgtccaagg tcaaggtcag tttctgcacc atcatctgta
     6781 acataaactt ccccatgttg ataagggctc attgtccctg ggtcaatatt aatatatgga
     6841 tcaaattttt gaattgtcac ctttagccca cggtttttta gtaagcgccc aagacttgct
     6901 gcaacaatcc ccttaccaat agaagagacc acaccacctg taacaaaaat atactttgtc
     6961 attaaaaact ccttaaatta aatgttaatc taacagttag tttacgggta aaacccaaaa
     7021 cacatgattg ataaataata ccagcttctc tagtcaaaac tagtagaaac tatctgatca
     7081 agggcctact cagaaaacta tctggaacca ttctgtatct ttcccaaaaa caaaatagcc
     7141 ccctatcact agggagcttt atctgacctc ataagaggtg cccgaataat agtttaaact
     7201 atatctgact atttgtcaaa tacaaaatcc ccatctaaac ctcttcttcc tcttcttcct
     7261 cttcttcctc ttcttcgttg atttctacat cctcatctaa atcttcatca ggaatgattt
     7321 cattaatctc agaatcatac gattctacct ctgatttttc atcgtcaggg ttttcttcat
     7381 catattctaa acttggtgtt tcttctgtga aatcttcatc ttctggatca tcatcattgt
     7441 aatcaatagc atcttcatca ccatccatga aagcattgac acgtttcttc ttacgttttg
     7501 gagcgccatc ttcatcttct tcaagagtga tgatttcctc gtcaatttca tcgatagcat
     7561 accatgaacg aagtccccat ttgttttcgc caagtggaat aaaacttccg tctgtattca
     7621 aatctgaata gaaaaatggc aatgactcac ggattgctga gtcagatttt ccaaggtagg
     7681 tttgaatatc atttaccaaa tcgctgaagt acatttcgtt atcacgacca cgttgctcta
     7741 aaatagcgcg agcaacttca atcatcgaaa gttcactttt ttcttgccca gcaaatactt
     7801 ctaattccaa agttattctc cttaattgtg taagactatc attttgttta gcaatattct
     7861 ttatagatag aaaatagata agtcatattt tcaaattttc gctatttact attttacgct
     7921 atttttagct attcgtcaaa agaataatca caaataaaaa gactgggaag cccaatcttt
     7981 tcaaaattta ataagcaatt attttacagt agctgagcta gtgataacgt caaccgcttt
     8041 tttcattgca atatcatgtt taagcatatc tgctgaaagt agaccgcgta cttggtcagc
     8101 ttccatatta tattctgaag caagatctgt gatttctttt tcgatttctt catctgttgc
     8161 ttcaaagcct tctgcagctg caattgcttc aataacaagg ttagttttaa cgcgtttgtc
     8221 agcatctgct tggtattgct tatgaaggtc ttcttcagtt gtacctgtta attggaagta
     8281 catttctggt gaaattcctt gacgttgcat attacccatg aactcattca tagcacgatg
     8341 tacttcatca tggaccattt cttctggtaa ttcaacaatt tctgcattgg caacagctaa
     8401 ttcgattgct acaccttcaa ctgcatcatc aaatgcaatt tcttttgctg attctagctc
     8461 tttgcgatat ttcgctttca actcatcaag tgtttcaact tcatcatcaa tatcttttgc
     8521 aagttcatca tcaagtgctg gaacttcttt agtttttact tcatgaattg ttgtaacaaa
     8581 tttagcatct ttaccagcaa gatcttctgc ttgataatct tctgggaatg ttacattaac
     8641 gtctacagtt tgaccagctt tagaacctac taattgttct tcaaatcctg ggataaattg
     8701 acctgaacca agttcaagtg agaagttatc tcctttacca ccatcaaatt caacaccatc
     8761 aactgaacct acaaagtcaa taacaactgt atcaccttgt gctgcttcac catctttaac
     8821 agtcaattct gcaaggttgt tacgctcacg ttcaacttta gcatcaactt cttcatctga
     8881 aacttctttt gaagcatcaa cttctactga aaggtctttg taatccccaa gtttaacttc
     8941 tggttttgta acaacttcag ctgtcaactt ccaatcttga cctttttcca ttgatacaac
     9001 atcaattttt ggttgagcaa caacgtctaa accaagttct gcaacagctg cttcgtaagc
     9061 ctttggcaac actaaattca atgcattttc gtagagtgct tcttcaccaa atttttggtt
     9121 gaaaactgta cgaggcatat gtcctttacg gaatcctgga acgttcaaat cttttttaac
     9181 tttgttgaat gcttgatcaa gagctggctt gatctcatct tgactaattg taaatgtgat
     9241 aataccacgg tttgtagctt tattttcaaa tgatgtagac attaagtcat ttctccttaa
     9301 aattatttag atacagttta gtttaccata atttaaaacg ttttacaaag tttttatgca
     9361 aaaaagaagc tttaaactgc ttaa
//
LOCUS       DATRNX010000075         9199 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRNX010000075 DATRNX010000000
VERSION     DATRNX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..809)
                     /locus_tag="VBC81_001456"
     CDS             complement(<1..809)
                     /locus_tag="VBC81_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6912492.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
     gene            complement(809..1711)
                     /locus_tag="VBC81_001457"
     CDS             complement(809..1711)
                     /locus_tag="VBC81_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6912493.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(1717..1845)
                     /locus_tag="VBC81_001458"
     CDS             complement(1717..1845)
                     /locus_tag="VBC81_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912494.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1978..3021
                     /locus_tag="VBC81_001459"
     CDS             1978..3021
                     /locus_tag="VBC81_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6912495.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            3125..5287
                     /locus_tag="VBC81_001460"
     CDS             3125..5287
                     /locus_tag="VBC81_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6912496.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            5250..5708
                     /locus_tag="VBC81_001461"
     CDS             5250..5708
                     /locus_tag="VBC81_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6912497.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5789..6052
                     /locus_tag="VBC81_001462"
     CDS             5789..6052
                     /locus_tag="VBC81_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6912498.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            6280..7215
                     /locus_tag="VBC81_001463"
     CDS             6280..7215
                     /locus_tag="VBC81_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6912499.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            7208..7981
                     /locus_tag="VBC81_001464"
     CDS             7208..7981
                     /locus_tag="VBC81_001464"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6912500.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7996..8394
                     /locus_tag="VBC81_001465"
     CDS             7996..8394
                     /locus_tag="VBC81_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6912501.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            8391..8822
                     /locus_tag="VBC81_001466"
     CDS             8391..8822
                     /locus_tag="VBC81_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6912502.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8863..9138)
                     /locus_tag="VBC81_001467"
     CDS             complement(8863..9138)
                     /locus_tag="VBC81_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6912503.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
BASE COUNT         3064 a         1428 c         1853 g         2854 t
ORIGIN      
        1 aagacacgat aaacataagg attattaggt ttagaaactt gacgagactc ttcaatgtgt
       61 aagactggta agttttgttg taacaattga ttatactcgg atttaattgt acctctaaca
      121 gttacccaag tattgtccgg ataacttttt tgatttccag ttgttagtag accataaact
      181 cctgaatcag caatacaatg aatgattcca aagcggaaaa taaattgata tccttcgtgt
      241 ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa ttgctcggga
      301 tagagataaa ttagttccat aacttccatg tagttttcag ttgtgatggt taatgtacca
      361 gagcctttat attttttaag ctctttttgc atttcttttt cataggctga agaagtaaaa
      421 taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact gacagtacca
      481 gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt cgagtctaaa
      541 ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat tgggcttgtt
      601 gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac ccaaataatt
      661 agttgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa ataggtataa
      721 tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct taattcaaaa
      781 taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa caatagataa
      841 taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta aaagaattta
      901 ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa gccatcactg
      961 gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct tcactacaca
     1021 atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg ctgtgtccga
     1081 tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt aaagtgccaa
     1141 atactagata gcgtcctgtg tcaaaaaatt catcgatggc atgagctaag gctagaaaga
     1201 ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta gcgtcttctt
     1261 ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg gctacaatgg
     1321 tagcacctac aaagcgtaat atcaggaagc gtatagaatt tccaaatgct gagtaggtcg
     1381 caaataaaac aataggatta ataataggag cagtagcaag aaacggaact gccgtgtatg
     1441 aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat tcgcaagaag
     1501 gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt ttcggtaaga
     1561 acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa atagttccaa
     1621 gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac cattgaagga
     1681 cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag aatcatcttt
     1741 ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag caaaactagc
     1801 gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg gcacctcttt
     1861 ctttatataa taacctatta tactatatta gcttattttt aacataaaaa aacaaatcga
     1921 ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg taagattatg
     1981 attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac aaaccaaagc
     2041 tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc tgatcaagtt
     2101 ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt ctctgcaccc
     2161 gaaattatcc tagcagcagg agcggttaga actaataata tccgtttatc tagtgcagta
     2221 acgattctct cttccaatga tcctattcgc gtctatcagc aattttcaac gattgacgca
     2281 ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga gtcttttcca
     2341 ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat ggatatgttg
     2401 ttagcaatta actcagcgac aaatctcgat tggaaaggtc atttgacaca aacagttaat
     2461 gagcgaccaa tttatccaag agcattacaa agacagttat caatatgggt ggcaacagga
     2521 ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt ttatgcaact
     2581 attggtggga atcccaaagc ctttcgtcaa ttggtccata tttataaaga agttggtagt
     2641 cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg atggattgaa
     2701 gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac cgtcgataat
     2761 attgctaagg gacgccctca ttggtctgaa atgactaaag agcagtattt acgttcagta
     2821 ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa aattatagga
     2881 cttgttgaag cgcttgagtt agaccgtttt atgttacatc taccagttgg ctctatgcct
     2941 cacaaagacg tcctaaatgc catcaaactt tatggtgagg aagtagcacc aattgttcga
     3001 aaatattttg aaaagaatta aagcagacct ggtctgtttt ttattgttag attataaaaa
     3061 cgctatcttg aaatcgcttt ttaaaactct tcgtaagtca aaaatatgct ataataaaag
     3121 cgttatggaa aatttaaata ttacacaaat tgctattgat ttaggtatta aagcgtcaca
     3181 aatcgaaaag gttttagaat taacagatga aggaaatact attcctttta ttgcccgtta
     3241 tcgtaaagaa atgacaggta atttggatga agttcaaatc aaatcaatta ttgaccttga
     3301 taagagtatg acagctcttt cagatcgtaa aactacggtc ttagccaaaa ttgaagaaca
     3361 aggaaagttg actcaagaac tcaaaaaggc tattgaagaa gcaactaaac tagcagatgt
     3421 tgaggaactc tacctacctt ataaagaaaa acgtcgtacg aaagctacga ttgcacgtga
     3481 agcaggtctt tttcctttag cacgtcttat tttacaaaat aaggataacc ttgaggaaga
     3541 agctcaaaac tatcttacag atggttttga gacaactacg aaggcccttt ctggagcagt
     3601 agatattctt atcgaagctt tttctgagga taataaatta cgttcatgga catataatga
     3661 gatctggaac tacagctcaa tcactgcagt tgttaaagat gagtcacttg atgaaaaaca
     3721 ggtcttcaaa atctactatg acttttctga aaaaataagt aaactccatg gctatcaagt
     3781 tttagcattg aatcgtggtg agaaaatggg tgttcttaag gttaattttg agcataacct
     3841 tgaaaaaatg tttcgctttt ttgctgttcg atttaaagaa actagccaat atatagatga
     3901 ccttattgta cagactgtca agaaaaaaat tgttccagct atggaacgcc gtatccgtac
     3961 agaactaagt gaaggagcag aagatggtgc aatttcactg ttttcagaga atcttcgtaa
     4021 cttattgtta gtctcaccgc ttaaggggaa aatggtgtta ggttttgacc ctgcctttcg
     4081 tacaggagca aaattagcag tagtagacca aactggaaaa ttaatgacaa cacaagtgat
     4141 ttatccagtt cctccagcta atcaagcaaa aatcgaacaa tcaaaaatag agttagcaaa
     4201 acttatcaaa gaattcaata tagaaattat tgctataggg aatggaacag cgagtcgtga
     4261 atcagaagca tttgttgcag aagttttaca agattttcca gacgtatcct atgtgattgt
     4321 taacgaaagt ggtgcctctg tttattcagc ctcagaattg gcacgtcatg aatttccaga
     4381 cttaacagtt gaaaaacgtt cggctatctc aattgcaaga cgtctgcaag acccgttagc
     4441 agaactagtc aaaattgatc caaaatctat tggtgtgggt caatatcaac atgatgttag
     4501 tcaaaaaaaa ttagcagaaa atcttgattt tgttgttgaa actgtagtta accaagtagg
     4561 tgttaatgtt aatacagcaa gtccagcctt gttagctcac gtttcaggtt taaataaaac
     4621 aatatctgaa aatattgtta aatatcgtga agagaatggg caaattaagt cacgtgctga
     4681 aattaagaaa gtacccagat taggagcaaa agcttttgag caagctgctg gattcttacg
     4741 tattcctaat gctaaaaatt tcttggataa tacaggcgtt caccctgagt cttatgaagc
     4801 tgttaaaaag ttgcttgacc aactgactat caaagaatta gatgatcttg caaaagagaa
     4861 gttacaacac cttgacttaa ttgcaacagc agaaagtatt ggggtaggtc aagaaacatt
     4921 aaaagacatt atagaagatc ttttgaaacc aggtcgtgat ttgcgtgatg attttgaagc
     4981 gcctgttttg agacatgatg ttttagatgt cagtgattta aaagttggac aggaattgca
     5041 aggaactgtt cgaaacgtcg ttgattttgg tgcttttgtt gatattggtg ttcatgaaga
     5101 tggtctaatc caccagtctc gccttattaa gcgaaaacgc gataaaaaaa ctagaaaaat
     5161 gcctccttta ccacatccaa gcaaatatct ctctgttggt gatattgtaa ctgtttgggt
     5221 tgtagaggta gatgcggagc gtggcagaat tggacttagt cttatcaagc cagacggact
     5281 tgactaatta tgttaagaag gtgtctatag aagattttgg atgtcagttt ttacatactg
     5341 cacattggaa taatcgctta agaacaaccg gaggacgctt ctttccaaac gatagacacc
     5401 ttgactttaa tcccaaaatt tatcgtgagt ttggtattga tatttttcgt aagattgtaa
     5461 gacatgagct ttgccattat catctctata tacaaggtaa aggatatcga catagggata
     5521 aagcttttaa agagttacta gaaaaagtcg atggcttacg atatacccct agagttacgg
     5581 ctgttaaagt gaattatcat cattactctt gccaatcttg tggtcaagta taccgacgta
     5641 ggagacgtgt taatttacgc aagtttgctt gtggttattg tcatggtcgc ttgatagaga
     5701 gtttttaaaa tcagtcgtaa ggcctattta attttactgt caaagtagtt ataattaaaa
     5761 tatagagtag atagaaagaa ggataggtat gaaatcaagc ttctacaaac aacgaaaagg
     5821 taagttggtc tgcggtgttg tcgccggtct agctgataag tataactggg atttagctct
     5881 ttcacgggtg ttgattgcac tgattttgta ttttaccaaa tttggtcttt tgctatatat
     5941 tttattagct gttttcttac cctataaaga agatattatt gaaacaagaa gacaaggtcc
     6001 cagaagacgt aaagatgctg aacctgttga tgatgacggt tggttctggt aattcatata
     6061 aaggagattt gctagacgaa tctccttttt tcttagcttt atttagacca ttttttcaaa
     6121 atatcttctt tttttcagaa aaacgcgtta gcgcttactt ttagtaaaaa gtatgatata
     6181 atgtgggtgt aaattaatga aagtgtttag tacctttgct caactttaaa ttgtagggta
     6241 tagcaatagg gatattcact tttataagaa ggagtaagta tggcagttac tgttcaaatg
     6301 ttagttgacc gcttaaagtt aaatgtcatc tatggtgatg aacatctatt atctaagcgg
     6361 attacaacag ctgacatttc acgtcctggt ctagaaatga cagggtattt tgattattat
     6421 gcaccagagc gtttgcaact tgttggtatg aaagagtggt catatttaat ggctatgaca
     6481 ggacataatc gttatcaagt gttacgtgag atgttccaaa aagaaacacc agctattgtt
     6541 gtagcacgtg atttagaaat tccagaagag atgtatgagg cggctaaaga taccggtatt
     6601 gctattcttc aaagtaaggc ccctacaagt cgtttatcag gtgaagtttc ttggtattta
     6661 gattcttgct tagctgagag gacaagtgtt catggtgtct tgatggatat ctatggtatg
     6721 ggtgttctga ttcaaggaga ttccggtatt ggtaagagtg agacaggact agagctagtt
     6781 aagcgaggac atcgtttggt agcagatgac cgtgttgatg tttatgctaa agacgaagag
     6841 acgctttggg gagagcctgc tgagatatta cgtcatttac ttgaaattag aggtgtggga
     6901 atcattgata ttatgagcct atatggagct agtgcggtta aagattcatc gcaagtgcaa
     6961 ttagctatat accttgaaaa ctttgaaact ggaaaagttt ttgatagact cggaaatggc
     7021 aatgaagaga tcgaattatc tggagttaag gttcctcgga tacgtattcc tgttaaaaca
     7081 ggccgaaacg tgtcagtagt tattgaagca gcggctatga atcacagagc taaacaaatg
     7141 gggtttgatg ctacccagac ttttgaggat cgtttaacac atttgattag tcagaacgag
     7201 gtcaatgatg attaatccag tagcaataca attaggcccc ttttctattc gctggtatgc
     7261 tatttgcata gtgtctggta tgttgttagc agtttactta gcaatgaaag aggcccctcg
     7321 taagaacatt aagtcggatg atattctaga tttcattctc atggcttttc cactatccat
     7381 tgttggagct cgtatttatt atgttatctt cgaatgggct tattactcta agcacccagt
     7441 agagattatc gccatttgga acggtggtat tgctatttac ggagggttaa ttaccggagc
     7501 cattctcctt gtgatttttt catatcgtcg cttgattaat cccattgatt ttctggatat
     7561 tgctgctcca ggagtgatga ttgcccaagc cataggtaga tgggggaatt ttattaatca
     7621 agaagcatac ggtagggcag taaaaaatct gaattactta ccaaacttta taaaaaatca
     7681 aatgtatatt gatggagctt accgagtacc tacattttta tatgagtctc tttggaactt
     7741 cctaggtttt gtcatcatca tgtctattcg tcatcgaccg cgcactttaa aacaaggtga
     7801 agtggcatgt ttttatttag tttggtatgg atgtggacgg ttcattattg aagggatgag
     7861 gacagatagc ctttatttgg cgggtttgcg cgtgtcacaa tggttatcag ttattttagt
     7921 tattatcgga attgtcatga ttatttatca tcgtcgtgaa caacatattt catattatta
     7981 aacagaggag gcattatgat agaaattgct gtattaatta ttgccattgc gtttgttgtt
     8041 ttagttttag gaatactttt cgttctgaaa aaagtatcag aaacgattga ggaaaccaag
     8101 caaactatta aagtattaac aagcgatgtt aacgtgactc tttaccaaac taatgaaatt
     8161 ttagccaaag ctaatgtttt agttgatgat gttaatggta aagtatctac tattgatcct
     8221 ctttttgtag ctattgctga cttatcagaa tctgtatctg atttaaacct acaggcaaga
     8281 cacattggac aaaaggcaag tagtgcaacc tcaagtgtta ctaaggcagg gtcagcttta
     8341 gctattggaa aagcggcttc aaaaattttt cgtaaaaaag gagacaaaaa atgagtaaat
     8401 tatttaaaac tttagttatc agtgctgcat caggtgcagc agctgcttat tttttaacaa
     8461 caaaaaaagg aaaagagcta aggaaaaatg cagaaaaatt ctatggagaa tataaagaaa
     8521 atccagaaga atatcatcaa atagctaaag ataaagcaag tgaatattca aatttagctg
     8581 ttgatacttt taaagattat aaaggtaaat ttgaatcagg tgaattgaca acagaggata
     8641 tcgtctcagc cgttaaggaa aaaagcggag aagtagttga ctttgctaat gattttgtca
     8701 atcaagctaa atcaaaattc tcagacgagg atactgctaa aaaagaagat aaggctcctg
     8761 aaacaaaagt agaagatatt gtcattgatt ataaagaaaa cacagaagat aaagaaaaat
     8821 aaaaaagaag ctactataag tagcttcttt tttatgagat ttttattgtc tgagtgacca
     8881 aatgaatgaa gagactgcag atgtaactgc tagacttgcc agcatcgcta gtaagaaact
     8941 ccacacaggt gttaaactta gtgctagtaa taagaaagag attagcacag tggttatact
     9001 gaagagagcg atacgagcaa aaaagactag ctcacgcaac ttagcactgt gatcaaattc
     9061 ttgaaatact tgaaattttg gttgagcttg ctctgttttg ctttcgtgag tttgctcttg
     9121 cttaagtggt gatgacatag ctttctcctt tgatactagc atatcatatc acaaataata
     9181 gagtttgtca ctagttttt
//
LOCUS       DATRNX010000076         8855 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRNX010000076 DATRNX010000000
VERSION     DATRNX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..310)
                     /locus_tag="VBC81_001468"
     CDS             complement(<1..310)
                     /locus_tag="VBC81_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEO6912504.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     S"
     gene            complement(450..1877)
                     /locus_tag="VBC81_001469"
     CDS             complement(450..1877)
                     /locus_tag="VBC81_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6912505.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(2027..3760)
                     /gene="ptsP"
                     /locus_tag="VBC81_001470"
     CDS             complement(2027..3760)
                     /gene="ptsP"
                     /locus_tag="VBC81_001470"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6912506.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3765..4028)
                     /locus_tag="VBC81_001471"
     CDS             complement(3765..4028)
                     /locus_tag="VBC81_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6912507.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            4411..4635
                     /locus_tag="VBC81_001472"
     CDS             4411..4635
                     /locus_tag="VBC81_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6912508.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            4713..6872
                     /gene="nrdE"
                     /locus_tag="VBC81_001473"
     CDS             4713..6872
                     /gene="nrdE"
                     /locus_tag="VBC81_001473"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6912509.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            7075..8034
                     /gene="nrdF"
                     /locus_tag="VBC81_001474"
     CDS             7075..8034
                     /gene="nrdF"
                     /locus_tag="VBC81_001474"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6912510.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      8119..8217
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8274..8837
                     /gene="thiT"
                     /locus_tag="VBC81_001475"
     CDS             8274..8837
                     /gene="thiT"
                     /locus_tag="VBC81_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6912511.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
BASE COUNT         2788 a         1694 c         1470 g         2903 t
ORIGIN      
        1 tagaatctgc taattgagga cgtagaataa tgtattgatt gtcataaaat tttttatgct
       61 caaaactttt ttgattccac tttaaaatat cagcgcttag acttttgtca tatcctttag
      121 gttcaagaaa aagaacacgt tcagttttct gtcttttcac cacttctacc gttttctcgg
      181 ctatgcgaat ttctttttca acaacagctt gttgaagttt tatttcctta tgtttcaaaa
      241 acgaaaaacc ttgccaagca agaatcgcta taataattag actaaaaata cctataatta
      301 atttcttcat ataatgactc ttttgttatt ttttatgaca attatatcac aaaaataaaa
      361 ccactttaga agattatata attctttatt tttaatgtaa aagaggttta taaaacgact
      421 caactgacaa tcgttgagtc gttttacatc tatttcatat caaaaacaat cgattttaca
      481 tttgtcattg cttcaattga atatctgata ccttgaacac ctgcaccaga tcctttgagt
      541 cctaagaatg ggaagttatc aggaccacgt ccagtcttat tgttaatgtg aactgtacca
      601 acttcaagtt tatttgctat atcaaatgct ttttggaaat cacgtgtaaa gactgatgat
      661 tgtaatccaa aatcagattt gttggcaata gcaacagctt cttctgcatc cttgacacga
      721 ataattggga gaattggtcc aaaaggctct tcccaagcta gtttcatatc taaagtaaca
      781 tgatcaaaca atcctggagt taatagacga ccatcacgtt taaattcatt caattctttc
      841 gcaccttttt gacgtgcatc tactactaag ctttcaataa agtcagctga attatcatca
      901 ataaccggtg tcaccgttgc attatcaaat ggatcaccta ctgataattt tgctacattt
      961 tcagatattt tttctgccaa ttcatctgca acttcttcaa cgacaagcac acgcttaatt
     1021 gccgtacagc gttgtccaga gtaatcataa gcacctgcaa cgatttgttt agcagcgtta
     1081 tcaaggtcag catcagctaa gacgatacct gcatccttac cgcccaactc aagcataatt
     1141 ggacgcattc ctgccaactt accaatacgt tgtccaactg gcgttgatcc tgtaaagtta
     1201 ataaaattaa cttcttcatg ctcaacaatg taatctccaa tctcagaacc gcgtcctgta
     1261 atagtattaa agacacctgc tggaagacct gcttctgcaa aagcttttgc taaaacaagt
     1321 cctgagactg aaccttgtgt tggtggttta aacatcacaa cgtttccacc aattagagct
     1381 ggcgcaattt ttgatcctga gaggttaaca gggtaattat aaggtgcgat tgctaaaacg
     1441 atacctactg gttgacgacg aataacagcc aacttatgac ctgttgaagc atccattttt
     1501 ccaccttcgt cagctgaagt tgataaacga attccttctt ctgctgcata acgaataaga
     1561 tcagctgtcc ttacaacctc agttactgaa gcattgtaag ctttagaaat ttcttttgct
     1621 aaaacagtag caatttcttc tttatcacgt tcaataatgt ctgcggcttt atgaaggtat
     1681 tgtgcacgtt catatactgt taaagcagcc caagctggta aagcctcacg acccgctttc
     1741 atagcatgat caacttcttc tcgagtcatc gctggcacga atcccaatga agaatcatca
     1801 attggtgaca aaatctcaat ctgattaaca gatgatttcc attcgccatt gacataattt
     1861 tgatattctt ttgtcaaagt aaatctcctt ttacattgta ataggtttat tctatcactt
     1921 tatctattta tttcaattat ctgacatatt tttcttagaa acttaacaat atccaaactt
     1981 tcaataaaaa agtagagatt ttttaatccc tactttttta ttattctcag tcaaagtcaa
     2041 cgtattcttt ttgaagttcg ataacttctt ccatagttga acactctgaa agagcacgat
     2101 tagcgtattc ttccatttta gctgtgtcca attttttcat taagctacgt gtacgaagta
     2161 ctgaggttgc tgacattgag aattcatcca atcccatacc aacaagaagt ggaaccgcag
     2221 tttgatcacc ggccatctca ccacacatac ctgcccattt accttcagca tgagcagctt
     2281 tgataacgtt gttaatcaaa cgaaggattg atggattgta tggttgatag aggtatgaaa
     2341 cttgctcatt catacggtcc gcagccattg tgtattggat aagatcatta gtaccaattg
     2401 agaagaaatc aacttctttt gcaaattggt ctgcaagcat agctgccgct gggatttcaa
     2461 tcataatacc aacttcaatg ccttcagcta ctgctacacc gtcagctaac aagttcgctt
     2521 tctcttcttc aaatatagct ttagcagcac ggaattcttt aagcaaagca accattggga
     2581 acatgatgcg tagctgtcca tgaactgaag cacgaagaag tgctcggatt tgtgtgcgga
     2641 acattgcatc accagtttca gaaattgaaa tacgcaatgc acggaatcct aagaatgggt
     2701 tcatctcttt tggaagatca aagtaaggaa gctctttatc tccaccgata tccattgtac
     2761 gaacaacaac tggtttacca ttcataccct caagaactgc tttatatgct tcatattgct
     2821 catcttctgt tgggaaatct tgtgaatcca tatataagaa ctctgtacgg taaagaccaa
     2881 ctgcttctgc tccattttcg ttaacaccct caacatcttt aggtgtaccg atattagcag
     2941 ctaattcaaa atgtttacca tcagcagtaa ctgtttgtgc atccttaaga agtgcccatt
     3001 cagctttttg cttggcataa gcttcacctg ctgccttgaa ggctgaaatt tgagcttcag
     3061 ttggttcaat gataacttca ccagtaatac cattaacagc aatcaactga ccatcttgaa
     3121 cacgttcagt aatatcattt gtaccaagaa cagctgcgat ttcgagtgta cgtgccataa
     3181 tagctgagtg acttgtacgc cctccaatat ttgttacaaa agctttaaca aattgtttat
     3241 ttaattgagc tgtatcagaa ggtgtcaaat cgtgtgcaat aacgattgat tcttcattga
     3301 tagttgccgg gtttggtaat ttaacaccca aaagatgtgc taagacacgt ttggcaacat
     3361 cacggatatc tgctgcacgt tcttgcatgt atgggttatc ttccatacct tcaaagatag
     3421 tgatgaacat atcagtcact tctttaagac ctgtttctgc atttacttgt tttgcacgaa
     3481 tagtctcttt aatttgatta atcatttctg ggtcagaaag aaccattaaa tgagcatcaa
     3541 atacagctgc ggcttcttcg cctaagcttt ctactgcctt ctcacggata acagaaagct
     3601 cgtcttgtga agcttggagt gcaacatcaa ggcgagcttc ttctgcgttt gtatcttcga
     3661 ctgttacagt ctcaaatgac aaatccggtt ggacaagtag atatgcttta gcaactgcaa
     3721 caccatcaga ggctgcgatt cctttaagca tttctgtcat tttcttatgc caatccttct
     3781 tttgtcattg tttcttcgat agctgcaatt gcatcatctg catctgcacc ttctgctgaa
     3841 atagtaacat cagcaccttg accaacacca agactcataa cacccatgat tgatttaagg
     3901 tttactgctt tacctttgta atcaagcgtg atgtctgaag caaatttgct agctgtttgg
     3961 acaagcaaag tagctggacg tgcgtgaata cctgtttctg caacaatgtg gaaatctttt
     4021 gaagccatat tatggttctc cttttgtcat ttgtaaatat ttgagtcacc tattggtaac
     4081 ccttacaagt taaaagtata acatattgct ttttattttt caagattaaa tgtctcaaac
     4141 agaaaaaaac ttgaatgttt tccacagctt ttcatattaa aaatgagagt cttagaattt
     4201 aaaaatagaa ttctaaaaat gattttttta tgttcttctg aaaggaattg gaaaacaggc
     4261 tgaaagcttt taaactaacc ctaaaaacga aatatagtgt ctcattttat tttaagacac
     4321 tatatattgt gttttgttag aaataaaaca cttgcttttt ccattctttt tttatatgct
     4381 attaccatga aaaaaaatta ggaggacaac atggcagcta ttactgtttt ttcgaaaaac
     4441 aactgtatgc aatgtaaaat gactaagaaa tttcttgacc agcacggcgc tgattttgaa
     4501 gaaattaata ttgatgaaaa accagaaaaa attgaatatg tgaaaaatct cggcttttct
     4561 gcggctccag tgattgaagc tggtaatgtt gttttttcag gtttccaacc aagcaaacta
     4621 aaagaacttg tttaagcaaa ttagagtctg ggaataaagc aattaattgc tttattccca
     4681 ttttgtattg ttaagttaag aggataaatt caatgagttt aaaaaatatt ggcgatgtat
     4741 cttactttcg cctaaataat gaaattaacc gtcctgttaa tggacaaatc ccactccata
     4801 aagataaaga agccttaact gcttttttca aagaaaacgt acagcctaat tcaaaagctt
     4861 ttgactcaat cactgataaa attgcttacc tccttaagta tgattatctt gaagaagctt
     4921 tcctcaataa atatcgtcct gaatttattg aagaattatc taccaaactc ttcgataaaa
     4981 agtttcgttt taaatccttc atggcagcct ataagtttta tcagcagtat gcattaaaaa
     5041 caaatgatgg agaatactat ttagaaagta ttgaagatcg cgttctcttt aacgctcttt
     5101 attttgcaga cggtgatgaa gaactagcaa ctgacttagc tctagaaatg atttcacaac
     5161 gctatcaacc tgcaacacca agttttttaa atgctggtcg aagtcgtcgc ggagaattag
     5221 tctcatgttt cttaatccaa gtaactgatg atatgaatgc tattggtcgt tcgattaatt
     5281 cagctcttca attatctcgt attggtggtg gggttggaat atcacttagt aaccttcgtg
     5341 aggctggagc tcctatcaaa ggttttgctg gtgctgcttc tggagtcgtt ccagtcatga
     5401 aattatttga agacagtttt tcttactcca accaacttgg acaacgccaa ggtgctggag
     5461 ttgtttacct tgacgttttc catccagata ttatttcatt cctatcaact aaaaaagaaa
     5521 atgctgatga aaaagtacgt gttaaaacat tgtcattagg gattactgta cccgataaat
     5581 tctatgaact agcacgtaac aatcaagaaa tgtacttgtt tagtccttat agtatcgaac
     5641 gtgaatatgg tgttcctttc agctatattg atattaccga aaaatatgat gaattagtcg
     5701 ctaaccctaa tatcactaaa acaaaaatca atgctcgtga tttagaaaca gagatttcta
     5761 aacttcaaca agaatctggt tatccttaca tcatcaatat tgacactgct aatcgtacta
     5821 atcctgttga cggaaaaatt attatgtcaa atctttgctc tgagattttg caagtacaaa
     5881 aaccaagtct tatcaatgat gctcaagaat accttgaaat gggaactgat atttcttgta
     5941 accttggctc aactaatgtt ttaaatatga tgacatctcc tgattttgga aaatcaatta
     6001 aaacaatgac tcgtgctcta actttcgtta ctgactcatc aaatattgaa gctgttccaa
     6061 ctattaagaa cggaaatgct caagcacata catttggact tggagcaatg ggactccact
     6121 cctatttggc aaaaaatcat atcgaatatg gtagtccaga atcaattgaa tttactgata
     6181 tttatttcat gctaatgaac tactggacct tagtagaatc aaacaatatt gcccgcgaac
     6241 gtcaaacaac atttgtaggt tttgaaaaat caaaatacgc tgacggtaca tactttgata
     6301 aatatgtatc tggaaaattt gttccacaat ctgacaaagt aaaatcccta tttactaatc
     6361 attttatccc cgaagctaaa gattgggaaa accttagata cgcagttatg aaagacggac
     6421 tatatcatca aaatcggctt gcagttgctc cgaatggttc tatttcgtat atcaacgatt
     6481 gttcagcatc tatccaccct attacacaac gtattgaaga gcgtcaagag aaaaaaatcg
     6541 ggaaaattta ttatccagct aatggcttag ctacagatac tatcccttat tacacttctg
     6601 cctatgatat ggatatgcgt aaagtaatag acgtctatgc cgcagcaact gagcacgtag
     6661 accaaggtct ctcaatgaca ctctttttac gaagcgaatt accaaaggag ttgtacgagt
     6721 ggaaaacaga aagtaagcag actactcgcg acctttcaat cctacgtaac tatgctttca
     6781 acaaaggtgt gaaatcaatc tactatatcc gtacttttac agatgatagt agcgaagtcg
     6841 gagctaatca atgcgaaagt tgtgtcatct aacgaagaaa ctctacttaa aacatcagaa
     6901 actagactga gataaactat ctcagtttca tgatgttatc ttattttcta aggtattttc
     6961 atctttattt ttctaaaaat cgataatact tgaaatattg ctagatcaga attctatatt
     7021 ctactcttct tccatcatca ttatataaaa acctatttac cattggagaa aaccatgaca
     7081 acttactacg aagctataaa ctggaacgaa attgaagatg ttattgataa atcaacttgg
     7141 gaaaaactaa ccgaacaatt ttggctcgat acacgtatcc ctttatcaaa tgacttagac
     7201 gattggcgca aactttccgc tcaagaaaaa gatcttgttg gcaaggtttt tggaggctta
     7261 accctacttg ataccatgca atcagaaact ggtgttgaag ctattcgtgc cgatgttcgc
     7321 acgcctcacg aagaagctgt cttaaacaat attcaattca tggaatctgt tcacgctaaa
     7381 tcttattctt caattttctc aactttaaat actaaatcag aaattgaaga aattttcgag
     7441 tggactaata ataatgagtt ccttcaagaa aaagcacgta ttatcaatga catttatgct
     7501 aatggaaatg cccttcaaaa aaaggtggct tccacctacc tcgaaacttt ccttttttat
     7561 tctggctttt tcacacctct ttactatttg ggaaataata agttagcaaa tgttgctgaa
     7621 atcattaaat taattattcg tgatgaatct gtacatggta cttatatcgg ttacaaattc
     7681 cagcttggtt ttaacgaatt accagaagat gagcaagaga attttcgtga ttggatgtat
     7741 gacctccttt atcagctgta tgaaaacgaa gaaaaataca ccaagacact ttatgatggc
     7801 gtaggatgga ctgaagaagt tatgaccttt ttacgctaca atgctaataa agctcttatg
     7861 aatttaggac aagatccttt attcccagat acagcaaatg atgtcaaccc aattgttatg
     7921 aatggtattt caacaggaac atcaaaccat gacttcttct ctcaagtagg taatggttac
     7981 ctacttggta gcgttgaagc tatgcatgat gatgactata actatggatt ataagaaaaa
     8041 gacactgatt tagtgtcttt ttctagttaa atacaaattt cttgacaagc aaaccctttc
     8101 aagatagaat gttagagtaa atacacaagg gagtgccttg agctgagatt gcagatatgc
     8161 aaaatcctct aacctgatct cgttaggacg agcgtaggaa ttgtggtttg aattggttaa
     8221 acatgacaaa tgacaaaaga atctcctttg ctgtataaaa aaggagattt tttatgtcaa
     8281 aaaataacaa cactacctgt cttattgaaa ctgctatttt tgcagcgctt gctatggctt
     8341 tatcaatgat tcctgatttt gcgtcatggt tcactccctc ttttggagca attcctctga
     8401 tactttttgc attacgtcgt ggtacaaaat acggtctatt tgctggctta atttggggct
     8461 tattgcactt tgttctttcc aaagtatatt accttagttt atcccaagtt tttatagaat
     8521 acatcttagc atttataagt atgggacttg caggcgtatt ttctgctaaa tttaaagatg
     8581 cacttagcag cagctctaaa actaaagctt tatcattagc attatctgga gcaatattag
     8641 caacatttgt tcgttatgtt tggcattata tcgctggtgt tattttttgg gctagttatg
     8701 caccaaaagg tatgtctgct acattatact cattaagcgt caatggtact gctggtttgt
     8761 taactctatt ttttgtagtc attagtatta ttattttagt tatcagctac cctagcttct
     8821 ttttacctaa gaaatagaaa aaataaaagg actga
//
LOCUS       DATRNX010000077         8717 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRNX010000077 DATRNX010000000
VERSION     DATRNX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            96..635
                     /locus_tag="VBC81_001476"
     CDS             96..635
                     /locus_tag="VBC81_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6912512.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            715..1086
                     /locus_tag="VBC81_001477"
     CDS             715..1086
                     /locus_tag="VBC81_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6912513.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            1120..2466
                     /gene="glnA"
                     /locus_tag="VBC81_001478"
     CDS             1120..2466
                     /gene="glnA"
                     /locus_tag="VBC81_001478"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6912514.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            2614..3123
                     /locus_tag="VBC81_001479"
     CDS             2614..3123
                     /locus_tag="VBC81_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6912515.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(3281..4960)
                     /locus_tag="VBC81_001480"
     CDS             complement(3281..4960)
                     /locus_tag="VBC81_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6912516.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5014..5244)
                     /locus_tag="VBC81_001481"
     CDS             complement(5014..5244)
                     /locus_tag="VBC81_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6912517.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            5423..6115
                     /gene="tsaB"
                     /locus_tag="VBC81_001482"
     CDS             5423..6115
                     /gene="tsaB"
                     /locus_tag="VBC81_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6912518.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVSEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            6117..6524
                     /gene="rimI"
                     /locus_tag="VBC81_001483"
     CDS             6117..6524
                     /gene="rimI"
                     /locus_tag="VBC81_001483"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6912519.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            6600..7610
                     /gene="tsaD"
                     /locus_tag="VBC81_001484"
     CDS             6600..7610
                     /gene="tsaD"
                     /locus_tag="VBC81_001484"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6912520.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            7730..>8717
                     /locus_tag="VBC81_001485"
     CDS             7730..>8717
                     /locus_tag="VBC81_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6912521.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWST"
BASE COUNT         2790 a         1379 c         1776 g         2772 t
ORIGIN      
        1 aattggttta taaaagatat actagtaata aataaaacgt ttacaaaaag ggttttatgg
       61 attgatatgt tagaatatat agtgaaggga taggtatgaa aacgttaaaa aaattattat
      121 ctaattacaa gtttgatatt aaaaagttta aatttggaat gagaacattt aaaacaggct
      181 tatcggtctt tttagtttta ttggtttttc atttatttgg ttggaacggg cttcaaattg
      241 gtgctctaac tgctgtattt agtttaagag aagactttga taaaagcgtg cactttggtt
      301 tttctagaat tattggtaat agtataggtg gcttactctc cttagtattt tttgcattca
      361 atgagatatt tcatcaagct ttttgggtca ctttattaat tgttcctatt tgtaccatgt
      421 tatgtatcat gattaatgtt gcttgtaata ataaatcagg tattattggg ggaacagccg
      481 cattacttat tataacttta tctatcccaa gcggagagac aattctatat gtttttgcac
      541 gtatttttga aactttttgt ggtgttttta ttgcaatgat ggttaataca gatattgaaa
      601 tacttcggaa aaaactaaaa aataacaaat tatagtttga tgtcataaaa tctcacatga
      661 gagttgacat caaacttttt aaagtttata atggtttaat gaaaggaggc catcatgaaa
      721 gaaagagaac ttaggcgtac aatggctgta ttcccgattg gagctgttat gaagttgaca
      781 gatttgactg ccagacaaat tcgctattat gaggatcaag ggttaatcac acctgaacgt
      841 actgaaggta accgccgcat gttttcactg aatgatatgg accgcttact ggaaataaaa
      901 gacttcatta gcgatggttt gcatatttct gatattaaaa atgaatacat gcaaagacaa
      961 cataagtcca aagagaaaca aaagtcatta tcagatgccg aggttcgtcg acttttacaa
     1021 gatgagctcc gtaatcaagg gcgtttttca agtccttcac agcacattgg aaatatgcat
     1081 ttgtaaacat gcagtcaagc tagatattaa ggagactata tgactatcac agcagaagat
     1141 attcgtcgcg aagttaagga aaaaaatgtt accttcctcc gtttgatgtt cacagatatc
     1201 ttaggagtaa tgaaaaatgt cgaaattccg gctacagatg aacaattaga caaagtttta
     1261 tcaaataaag caatgtttga tggatcttct attgagggct ttgttcgtat caatgaatct
     1321 gatatgtatt tatatcctga tttagatact tggattgtct ttccttgggg agatgaaaat
     1381 ggagctgttg caggtctaat ctgtgatatc tatacagcag aaggagaacc ttttgccgga
     1441 gatccacgtg gaaatcttaa gcgtaatatg aaacgtatgc aagagatggg ctataagtca
     1501 tttaacttag gaccagaacc tgaatttttc ctatttaaaa tggatgaaaa tggtaaccca
     1561 acacttgatg ttaatgacaa aggaggctac tttgacttag ctccaacaga cttagcagat
     1621 aatacacgtc gtgaaattgt taatgtctta acacaaatgg gctttgaagt tgaagctagt
     1681 catcatgaag tagcagttgg acagcatgaa attgacttta aatatgatga tgttttaaaa
     1741 gcctgtgaca acattcaatt atttaaattg gttgttaaaa caattgctcg taagcatggt
     1801 ttatatgcta cttttatggc aaaacctaag tttggtatca atggttcagg tatgcattgt
     1861 aatatgtctc tttttgataa tgaagggaac aatgctttct ttgatccaga agatccacgt
     1921 ggaatgcaat tatcagagga tgcctactac tttttaggtg ggttaatgaa acacgcttac
     1981 aattacacag ctatcattaa tccaactgtt aattcttata aacgtttggt accaggatac
     2041 gaagcaccag tttacattgc ttgggcaggt cgcaatcgtt cgccacttat tcgtgtacct
     2101 gcatctcgag gtatgggaac acgcctagaa ttgcgctcgg ttgacccaac agcaaatcct
     2161 tatcttgctt tatcagtact tttgggatca ggacttgagg gaatcgagaa taaaattgaa
     2221 gcaccagaac caattgagac aaatatctat gctatgactg tagaagaacg tcgtcaagca
     2281 ggtattgttg atttaccatc tactcttcat aatgctctta aagcacttga agaagatgaa
     2341 gtggtaaaag cggccctagg aactcatatc tatactaatt tcttagacgc taagcgtatt
     2401 gaatgggcaa gttatgcgac ttatgtttca caatgggaaa ttgataatta cctagattta
     2461 tattaatcat aagaatgttt ataactagtt ttaggcttta ttttgtaaag tctaaaacta
     2521 gtttttttgt aaattattgt aaaaaattgc ctaaaacttg ttacaggttt tcatttgtct
     2581 gttatatgga tttgataaac taggagtcat cagatggagg aaaacatgaa cataaaacaa
     2641 ttaaagtcta aaacaatgct tggaacagtg gctttggtgt ccgctttttc atttgcttca
     2701 acaaatgcag atgcgaatac atataactat gcagtagatg tagactacct agcaagtgcg
     2761 gaggaaattg ctcaagcaca tccagctagt aatacgtttc cactaggtca atgcacatgg
     2821 ggtgtaaagg agatggcaac atgggcaggt aactggtggg gaaacggtgg tgattgggct
     2881 gcgagcgccg catcagctgg ttatacagta gggacacaac cgcgcgtggg ttctatcgta
     2941 tgttggactg atggtagtta tggacatgtg gcttatgtta cagctgtaga tcctgtcact
     3001 aataaaattc aggttttaga atctaattat gcaggtcatc aatggattga taattatcgt
     3061 ggttggtttg atccacaaaa tacagtaaca ccaggggtag ttagctacat ttatccgaat
     3121 taatctataa agaactcctc acatagacgg tataaaagta cgatactgtt tggagttctt
     3181 ttatattcta tagttatccc agaagtaatt tgatatatta acttattttg ctaatatagg
     3241 caattaaaaa gaacggagaa agtattttcc gttcttttgt ttatttatca ggggtaagga
     3301 ccataggaat aatgataggc tcacgctcag ttttttcata taagaatggg cgtaaggcat
     3361 taacaatagc tccatttact gattggatac ttgcatcttt atttttcagt gcaatacgaa
     3421 tagcattgaa taagatatgt tggctttcgc gaataaggtc cccagattct ctcatataga
     3481 taaagcctcg gctaaggatg tctggtccag ctaaaatcat ttttgaatcg aaatcaacgg
     3541 tagcaacggc aaggacaaca ccatcttcag ataagtcgtg gcggtcacgt agaactgccg
     3601 caccaatatc gccaatacca ttcccatcaa cgtagatatc ttgagcattg aaatgtccag
     3661 caatacgagc agagtcgctc gttaaagcta aaacatcacc gttttccata atgaagatat
     3721 tctctttagg aatacctgtg tcaactgcta aaccagcatg gactttttgc atgcgatact
     3781 caccgtgtac gggcatgaaa tactttggtt taatcaagcg taacattaat ttttgttctt
     3841 gttgaccacc atggccggag gtatggatat tgttaatttt accatgaata acatcgacac
     3901 cagcttcttg aatggtattg attaatttat tgacacttgt tgtattacca gggattgggc
     3961 ttgatgagaa aatcacagta tcaccaggct gcaaagtaac ttgacgatga gtaccattag
     4021 cgatacgagc aagtgctgcc atagactcac cttgactacc tgtacacata attaaaactt
     4081 cactagcatg aagattttta agttcgcttg gctcaatgaa ggtacctttt ggaactttaa
     4141 tatagccaag ttcaataccg ttaacaattg ccttttccat tgaccgtcca aaaacagcaa
     4201 ttttacgacc agttttaaca gcagcttcag cagcctgttg gagtcggaag atgtttgaag
     4261 caaaagaggc gaaaataata cgtccctcga taccttcgat aatcttcata atagattgcc
     4321 caacaatttt ttcggaatta gtgaaggttg gaacttcagc atttgtcgag tctgataaaa
     4381 ggcagaggac accatcttca cctagagcag ccatacgatg taagtcagcg ggctccccaa
     4441 caggtgtaaa gtcaaatttg aagtcacctg tacaaataac cttaccttga ggggtatgaa
     4501 taacaatacc taatggttca ggaatggagt gagtagtgcg gaaaaatgta acgcttaagt
     4561 ttttaaaagt cagctctgta ttagcgtgga tttcgtataa tgtagcgtca cgaagtaaac
     4621 catgttcttc taacttacct ttgattagag caagagcgag aggacctgca taaatcggta
     4681 aattagcttg ttttaataga aaaggaatac caccgatgtg gtcctcgtgc ccatgagtta
     4741 taacaagggc cttgatacgg tcaatgtttt caacgatata tgaatagtct gggataacat
     4801 aatctattcc caaaagatca tcttcaggga atttaatacc agcgtctaca ataataattt
     4861 cgtcttgata ttcgataccg taagtatttt ttccgatttc accgagacca ccaatcgcat
     4921 aaaccccaac ttcttcgggt tttaagttga tatttgacat aataagttta tcataatggg
     4981 tagattactc tagctattat gtttaactcc tttctagaac tcagtgagct cgaaaacacc
     5041 agtttctttt tcatattcaa ggtgtttgtc tgaaagtaac tcgatgaatt cgatattgta
     5101 agctgtattt tcttcaacta gtttacgtgc agcaatgcga ccttctaatt cattagctgc
     5161 atcaatagta acgtaaagtg ttttggtttg ctcacgacgt gggttgcgtt cttttgtttc
     5221 ttgatagaaa actttataaa tcataataat cctttcttat ctctcggttt ttctaactat
     5281 aaaaaaatgg gtcaatgccg ttaagtgaga caatgacaaa ccattattaa gtttttatag
     5341 agtaatagaa ttaaccttga ttcgttgcaa ttatcatgat tatatcataa aataagaggt
     5401 agaatcaagt aagggaagtt aaatgatgaa agttttagcc tttgatactt caagcaaagc
     5461 actatcagtg gctgtactaa acaatatgga atgtttagcg actgtcacta tcaatatcaa
     5521 aaagaatcat agcattaatt tgatgccagc cattgatttt ttaatgcaat caattgattt
     5581 agaacctcaa gatttggacc gtatcgtagt atcagagggt ccaggatctt atacgggctt
     5641 acgtgtagct gttgctacag caaaaatgct agcttatacg cttaagattg acttagttgg
     5701 agtatctagc ctgtacgctt taacaaatgg attttcagaa aatgatttat tggtaccact
     5761 tatagatgca cgacgtaaca atgtttatgt tggtttctat caaaatggtg atactgttaa
     5821 accagactgt cacacttctc ttgaagaagt cttacaagag gtggggaata aagccaatgt
     5881 tcattttgtc ggagaggttg cagcattttt tgatcagatt aagaaagcct taccacatgc
     5941 taaaattaca gaaactttac cttgtgcagt agcaattggg cgcaaaggac aaaaaatgaa
     6001 aagcgttaat gtagatgcgt ttgttccacg atacttaaaa cgtgttgaag ctgaggaaaa
     6061 ttggttaaaa aaccactgtg aaacgaatac agaagaatat attaagagag tttgacatgg
     6121 aatcatcaga acaagcactg gctatatgga gcgtactatc tgatgtctat gacaaatctc
     6181 cgtggagttt atcgcagatt tcagaagatt taaaaaaaga ttcaacagat tatttttttg
     6241 tttataatga cggtgaagtc attggttttt tggctcttca acaattggta ggtgaagtag
     6301 aaattactaa tattgctgtt aaaaaaaatt atcaaggtaa aggttatgct taccagttaa
     6361 tgtcaatgat cgccgatata gaggtgccag tttttcttga ggttcgttat tcaaatatcg
     6421 tagcacaaaa actttatgag aggtgtggtt ttgtagtttt aagaaagcgc aaaaattatt
     6481 accatgatcc aattgaagat gctatagtga tgcgcaaggg ataaatagtt gatgatagaa
     6541 aggatatgaa tagtcctcaa tactaatgga aaagacgata ttgatggatt aatatcatta
     6601 tgaaagatag atatatttta gcagtggaaa gttcatgtga tgaaactagt gttgctattt
     6661 taaaaaatga taaagagtta ctagctaata ttattgcaag tcaagttgaa agtcacaaac
     6721 gttttggtgg tgttgttcct gaagtggcaa gccgtcatca cgttgaagta gtaacaacct
     6781 gttttgagga tgctcttcaa gaagcaggta ttgttgctag cgatttggat gctgttgctg
     6841 taacatatgg tccgggatta gtaggagcct tattggtagg tatggctgca gcaaaagctt
     6901 tcgcttgggc aaataaatta cctctaattc ctataaacca catggcaggt catttaatgg
     6961 cagcacgtga cgttaaggaa cttcaatacc cattgttagc tttgcttgtc agtgggggac
     7021 atacagaatt agtatatgtt tctgaaccgg gagattacaa aatagtagga gaaactcggg
     7081 atgatgctgt tggagaagct tatgataaag taggccgtgt tatgggctta acttatccag
     7141 caggtcgcga gattgatcag ttagctcata agggtcaaga tacttaccat tttcctagag
     7201 cgatgatcaa agaagatcat cttgaatttt ctttttctgg attaaaatct gcatttatca
     7261 atttacatca taatgcagaa caaaagggtg aagcattggt tcttgaagat ttatgtgctt
     7321 cctttcaggc ggctgttttg gatattttat tggccaaaac tcaaaaagct ttgttaaagt
     7381 atccagtgaa aactttagtc gttgctggtg gagttgcagc taatcaagga cttcgggaac
     7441 gcttggctac tgatatttct cctgatattg atgtggttat tcctcctctt agattatgtg
     7501 gggataatgc aggaatgatt gcattagcag cagcgataga gtttgaaaaa gagaattttg
     7561 cttctttaaa attgaatgcc aaacctagtt tagcttttga gagtttatag gtgttcataa
     7621 tatcaaaacg cttgttttga tattttttat ttgttataat gattgaatat tcaaaaatat
     7681 tagcaaatga tattgtcgct atttttttaa ctaataaagg agttaaggaa tgttacattt
     7741 tgaaaatgat tataataaag gagctcatcc agagctttta aatgccctta ttgaaactaa
     7801 tgatcaaggt ctatcgggat atgggacaga tagttactgt caacaagcgg cagataaaat
     7861 tcgagaagtc tgttcctgtc cacaagctga agttgagttt ttagtgggtg gtactcaaac
     7921 aaatcaagta gttatcagta gtatgcttgc ttcttatgaa ggagttattg ctgcagaaac
     7981 aggtcatgtg agtagtcatg aagcaggagc catagaattt tcagggcata aggtattgac
     8041 tttaccttct cataatggga aattactcgc aagcgaagtc gcaacttata ttgagacttt
     8101 ttatgctgat ggtaattacc aacacatggt ttttccagga atggtttata tatcacatcc
     8161 aacagaatat ggaacacttt atagtaaagc agaactagaa gagttatcga agatttgtaa
     8221 acattatcaa atccccttat ttattgacgg tgctcgtctg gggtatggtt tggctgcgaa
     8281 agatacagat gttgatttcc caacgattgc agccttatca gatgtttttt atattggtgg
     8341 aaccaaaatg ggggctcttg ctggggaagc agttgttttc acaaagaaaa atcgtccaaa
     8401 acagtttact acaattgtaa agcagcatgg tgcgttatta gccaagggaa gattgctggg
     8461 gcttgctttt gatcgtttct ttacggataa cctttactta aaaattggaa agcatgcgat
     8521 cgacttagca gaggaattaa aaatcatttt agaagaaaaa ggttactcat tctatttaaa
     8581 atcaccaact aatcaacaat ttattattgt agaaaataca aaactagcag atttagcaaa
     8641 gaatgtagct tatagttttt gggaaaaata tgatgaccat catactgtaa tacgtctagc
     8701 aactagttgg tcaacta
//
LOCUS       DATRNX010000078         8707 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRNX010000078 DATRNX010000000
VERSION     DATRNX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            225..1295
                     /gene="xerS"
                     /locus_tag="VBC81_001486"
     CDS             225..1295
                     /gene="xerS"
                     /locus_tag="VBC81_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6912522.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(1338..2276)
                     /locus_tag="VBC81_001487"
     CDS             complement(1338..2276)
                     /locus_tag="VBC81_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6912523.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2273..2941)
                     /locus_tag="VBC81_001488"
     CDS             complement(2273..2941)
                     /locus_tag="VBC81_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912524.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(3051..5006)
                     /locus_tag="VBC81_001489"
     CDS             complement(3051..5006)
                     /locus_tag="VBC81_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912525.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(5008..5760)
                     /locus_tag="VBC81_001490"
     CDS             complement(5008..5760)
                     /locus_tag="VBC81_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912526.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(5786..6748)
                     /locus_tag="VBC81_001491"
     CDS             complement(5786..6748)
                     /locus_tag="VBC81_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6912527.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            6914..7401
                     /locus_tag="VBC81_001492"
                     /pseudo
     CDS             6914..7401
                     /locus_tag="VBC81_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            complement(7433..8281)
                     /locus_tag="VBC81_001493"
     CDS             complement(7433..8281)
                     /locus_tag="VBC81_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6912528.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(8414..>8707)
                     /locus_tag="VBC81_001494"
     CDS             complement(8414..>8707)
                     /locus_tag="VBC81_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912529.1"
                     /translation="NRVNLLNNNAKVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQES
                     YRGQGIGTRLQFDASRGFKKVILISEEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT         2902 a         1513 c         1322 g         2970 t
ORIGIN      
        1 aatgaaatta tgaatcagtt ttctcaaaat actctgtctt tactataaca aacatgttaa
       61 acgataataa gtaatactga tcatcataaa gaatggatat ttgatgtact taccgaatga
      121 attcctgttt ttaactttta aactttccga aaaactataa ttttcttgaa agttatgata
      181 gagtatgata tacttctatt tatagatata atggagattt tattatgaaa cgtgaattac
      241 tactcgaaaa aatcgatgaa ctaaaagaaa ttatgccttg gtatgttcta gaatattatc
      301 aatcaaagct ttctgttcct tatagtttca caacactcta cgaatattta aaagaatata
      361 gacgttttct tgaatggtta ttggattctg gtgttgctaa ttgtcatcac attgcagaaa
      421 ttgagctttc agttttagaa aaccttacta aaaaagatat ggaagctttt atactatatt
      481 tacgagaacg ccctttatta aatgctaaca caaggcaaaa tggggtgtct caaaccacca
      541 ttaacagaac tctatctgcc ttatctagtc ttttcaaata ccttactgaa gaggttgaga
      601 atgctgacgg ggagccttac ttttatcgca atgtcatgaa aaaagtctca accaaaaaga
      661 aaaaggaaac tttagcttct cgggcagaaa atattaaaca aaagttattt ttgggcaatg
      721 aaacgataga attcttagag tatatagatt gtgaatatca aaacaaactt tccaaacgtg
      781 ctttagcttt tttcaacaaa aataaagaga gagatttagc tattattgct ttgctactag
      841 cttcaggagt gcgtctatct gaggcagtaa acttagattt aaaggatatt aaccttaatg
      901 taatggttat tgatgtcaca cgaaaagggg ggaaaaggga ctctgtcaat gtcgctagct
      961 ttgcgaagcc ctaccttgct aattatttag acatccgtaa aaatagatac aaagcagaaa
     1021 atcaggacat tgctctattt ttatcagaat accgtggggt gccaaatcgt attgattctt
     1081 caagtgtaga gaaaatggtt gctaagtatt cccaggattt taaggttcgt gtaacacctc
     1141 ataaactaag gcatactctt gctactaggc tatatgatgc tacaaaatct caagtattag
     1201 ttagccacca actaggtcat gcttccactc aagttactga cctatatacc catattgtta
     1261 acgatgaaca aaagaacgct ttagataaac tatagtaaca taaataaagt tatgtaaata
     1321 ttttatttta cataacttta tttatgtata gaaacagcag tcccataatt aattttgcta
     1381 tccaccgaaa tggacaaatt cagactagat aagacttctt ttgtcatata taaccccatg
     1441 cctgttgcct tttggtgctc atgaccgttg taacccgtaa agccatcttc aaaaagtcta
     1501 gggatatcct cttcgagtat gccgataccg tagtcttgta ttctaatact ctcttctcct
     1561 atgcttatta ttatctttga ctcaggatta gaatatttta tggcattatc tagcacctgt
     1621 gaaagagcaa aagttaacca ctttttgtct gttttccaga tattatcgcc ttcaattatg
     1681 atagataagc ttttggatag acaaataacc ttatacgatt ttataatttc tactactact
     1741 tctctaaggc taacagcttc aaaacgaaaa tcgtcacgat attgtctaaa tttcaaaaat
     1801 gctaacaacg tttcaagata atgttgcaat ttcaataatt gttgttcaac ttccttagga
     1861 tcgagatgat ttgtctgggc cattaatgaa atagctgcca atggaacttt catttggtgc
     1921 gaccacatct ttatcataag tgcaacatga ttggtttgtt caattgtttc agaaactttt
     1981 tgcgcttctc tagcttctaa ttgtgtaata atattaaaat aagcgtaatc actcggttga
     2041 agttcgaaag aggggtcctg actactatta aaatatttga gatgtaacat ttttttcctg
     2101 taacgactgt attgccaaat actaattcct agtaaaacaa taacatttaa acctaaggaa
     2161 ttaaacaaat agggcattgg taaatgatat agatagaaac taataaaaaa taggacaaac
     2221 atcatgatat ataaaatata ccaaatcaag tgttctctta aaaactggcg tatcattgta
     2281 gtaaataccc aactcctctc actgtatgaa tgtaatcaaa acctattggg acaatttttt
     2341 tacgtaagcg tgtcatatta acattaagtg tattttgatc aataaaacta tcattttccc
     2401 aaagtttctc taatagagac tcttttgaga ctacttgttt aggatgcatg agcaagatag
     2461 ataggatttt attttctgtt ggcgataaaa taacctcttt atcttggcta gacaataacc
     2521 cttctcttgt caacgtaaat cccccaaaag ttaattcctg ttggataaat tgttgacttc
     2581 tccttaaaat agcagttagc ttagcatcta atacagctag agagaatggt tttgaaataa
     2641 agtcatcacc ccccatattt aatgccataa ccatatccat ttcatcatta ctagatgaaa
     2701 tgaaaataat aggaattgtt aaaaacttac gcaattctgc agtccagtaa aaaccattaa
     2761 aatagggtaa cgtaatatcc attagtatca aatcgggttg aaatgcgata atttcttgtt
     2821 tcacatcacg aaaattgctg acactagaga catgatagct agctgataaa tgatctttta
     2881 aaagtgacac aatcgtcata tcatcttcta caatataaat ttttccttgc tcttgtgaca
     2941 tatcccctcc taaaatagta cgaaatctct ttctttcatt tagtataaca aattttttag
     3001 tctatcggtt tctattaatg caaaaggatt ggtaaaataa ccaatccttt ttagcgttca
     3061 ataatatgat aataagttcg actagttata cggtaaatga taaagtaaat tatagaaatc
     3121 gcaagtactg tcaaaccact tactacatat acgattgttg agttaagcac cccaaatacc
     3181 aaaagcatct gttttagcat tggaatagca actccaaagt gaataactgc taccactaaa
     3241 ggttggaaga agaaaatcat aatttgagaa ttaatagtcc gtttaactaa ttttttgctc
     3301 atacctactt cttgtaagat acgataagat ctcttatctt catgaccttc tgagtattgc
     3361 ttatagtaaa caattagagc tatacctagc aaaaagctta tacctaacag aaatccagta
     3421 aataataacc caccaaacat ttgataacct tcttttagaa aactctgttt agtgctaata
     3481 tttccaacat acttaccatc tacctctatt atgtcgtttt tagaaattga atttatctct
     3541 tgactattca aatccaaata tgccttaaag gtttttggaa aagtattgat gttcttagta
     3601 taaggtaaat atgcttttct aatattgtca atttgtttgt tgtttgcaaa tataatcaaa
     3661 cccgggttgt aagtatttgt agtctctggg acataagcct cttttaaatt ttttacaacg
     3721 tcaaatttat ttcccaatag atttattttt ttcaaacggc tatcaccttt ttgaacaaag
     3781 taagccactt ggttatcttt taattttggt aattggtgac ctaaacgacg gaaatcattc
     3841 tgagtaatta ggtacataaa acccgttttt gtgatatcaa catgtttgac gtttttagat
     3901 gtgatattaa gttcacttga ttgactaact ggcatcgaaa tcattgtctg attataagtg
     3961 atagcttcct tagatgactt acctagtttc ttcaaaatct tttcttcaaa tatatttttc
     4021 gcgtcacctt ttgaattatc tattgataaa cttactgatt ttggaaatag tccggtaaca
     4081 acattttgtg tatttgaata gagtgagact gttgtagcaa tagtaactag agccataaca
     4141 gctaataaag tgatacttgc taaccctact gcattttgct tcattcgaaa aatcatttgc
     4201 gaagttgata caaaatgctc agatttataa taataatgct tgttttgacg caaccttttt
     4261 aagtaccatg tcataaaact aatataaaaa agataagtac cagcaataac taaaagtact
     4321 gcaaagaaga aacgatagat aacagctaat gcaggtgctt tacctgaagt aagagccatt
     4381 gtataagcga tggcgatagc tactaacgct aaaattgcaa gtataagatt accttttggt
     4441 tctttttctc cctgttgttt ctttctaaaa agacttaatg gggatgataa atgaacatgt
     4501 cgaataactg gaacttctaa agtcagaaaa atacctgtaa atataacgat acaaataata
     4561 aatggccata aacttaaact aagatttagt gcatgatagt taataatgtt gacaaaaatt
     4621 aaataaataa atttagcaaa aaaagcacta aacagacttc ctatagaaat aagaaatata
     4681 taaataataa acagctctag actagctaca cgcgcaactt gacgtttatt catccccaaa
     4741 atgttataca gtccaaattc actactacgc tgtttcatca agatattgta gctataatgt
     4801 tccatgacta ccgcaaagat tgacaaaaca atcactccaa gaaaaagaac cgttgccgct
     4861 gtccccatat ctcttccaac agcactcatt aagattagta gcgtagaaca agtcaatgaa
     4921 taaagtaata aactggctaa gaggaatggt atgtactggt ctatagaatg ttttaaatta
     4981 tgccaggcta ttttgagata aaacatatta ttcaccccct aatatggcag acattgttaa
     5041 agaaatatct ttattgaatt cgctattatt cttattacca cgataaagtt ggtgaaaaat
     5101 acgtccatct ttgataaata aaacgcgttt ggcatgacta gcagcatttg ctgaatgggt
     5161 taccatcaaa atagtttgtc catccaagtt aatagtttca aataaattca gcaagtcttc
     5221 tgaattacgg taatctaacg ctgctgttgg ttcatctgct aataaaattt gagggttggt
     5281 tatcaaacta cgagcaatcg ctacacgttg cttttgccca ccagagagct cgaaaggtct
     5341 cttatctaat aagtcatcaa ttctcagatg agaagataat tctgacaaac gatgatccat
     5401 ttctttgtaa cgtttacgat caagaactaa aggaagatag atattatctc ttacagagag
     5461 agtatccaaa aggttgaagt cctgaaagac gaaaccaaga tttttcaaac gaaaactcgc
     5521 taattttgcc tctttaattt tcgtaatatc ttccccattt aaaatcactt gtccatttgt
     5581 cggtttttct aaagtagcta aaatatttag cagagttgtt tttcccgaac cagactctcc
     5641 cataatagca atgaattccc cttgttcaac tttaaaatcg acgtcttgta gagcccttgt
     5701 ctcttctttt gaaaaacgtg tacgaaaaac tttttctaag tgattgattt ctaataacat
     5761 ataattctcc tttatttatt ataccctaat tttgattgat tctactcttt aaccaagact
     5821 gtgactcttg taaaggaagg ctggttactt gatctggtaa gataggggtg ttctcgtatt
     5881 tatatccttg tcgatcacta actattcctg tagttaaagc taatctagca ccgtcgtaaa
     5941 gcatgaaagt ttcattaaca gtcgtatatc ctgctgtagc ttgaccaaag cttttaacat
     6001 taggtaatcc tttaaatgat aaaaaagtca tttctgccga actagcagtt ttatgattag
     6061 ttataatagc gattggaaca tgcttagtat tgattgtttt cctagatatt tttaatgctt
     6121 ctagcggtat atttttcatc gtgattgttt tcttatttcc atacttatca gtataatgaa
     6181 atagggtatc atttggtaaa atagaggcta accccccaat catagggatc atattgccgc
     6241 cagtattatt cgataagtct aaaatgagtc ccttataact tttttccatc aatatatttt
     6301 gtaatttacc actataatgg ctaatagatc gtttatctaa cttacttata gaaggaatag
     6361 taattaagag aatgtcttcg tttaatcttc gatattctgg atatcgacta tctagggtat
     6421 ctcttcgtac agtctctttg gataatattt gactatgttt accgcctcca taccgtactg
     6481 cttcctgtac aatgttgcga ctctcttgat aagattttga cgttttcaac tttttttctg
     6541 atcgttgtct tataatttgc cattgacgac cttgcgaata caaaccacgc tgttcaacac
     6601 gatctaaaat aacacgtcca taacgttcgc tactaggagg taatagataa atatttaatg
     6661 ctgaaccata ataatgaact accactccta aaattcctaa aactataagc attggaacta
     6721 caaaaagtaa aactattttt cgccgcatct cttgctcccc ctttctcttt aagtctatta
     6781 tatggcaaaa tggtttacta taaccttaca tcttaagacc tatttcttac aaatttgaaa
     6841 gttttaaaag ctttataaac tataattttg atataatatc ttacataaca aatggaaagt
     6901 acggtgaaaa catatggcca agacaagtaa aaaattgaca aaatctttag ggcctattgg
     6961 taaattaatt tcaataatcc ctgacacaac tgaacttatt ggaaaagcaa ttgataattc
     7021 tcgtccaatt attgaaaaag aacttgaccg tcgtcatgaa aaaaacagac ttatactcag
     7081 cttgataatg tagtacacct tgatatcaat gatgcgcaag tttacttaga gcaacgccat
     7141 tttaatgtat caaggattgt tgctgaacca aattcaaagt gggcaaccct acatcctaat
     7201 caagttgtga atatgtcgcc taaagctggt agggtcagaa tcggttcatt agtgaagttg
     7261 tactatatta cagaggatat ccttgaagaa agtaagacac tacttttatc taaatccgaa
     7321 aaaagtaaag cacgacaaca acggttaaca gaagtattga gtacccctag tcaattactc
     7381 aaaaaaaatc gaaataaata agcatgatcg aattgaccat gcttatttat ttttaccaaa
     7441 caggttgatc taaaccttga gatgtttttt cgataacttt tttaaccgca tctgtatggt
     7501 aagctttcac aacttcttta atggctttag cttttgattt gtcttgccaa cctttttgag
     7561 caactaaaag gttgtaccat tgttttgcat ttttactttt tggttcaata aaaatagctg
     7621 atttaggatt tattcctgct tctgtcacaa aatcattatt aataactgct gcatctgtgg
     7681 aatccaaaga acgtgcggtc tgtgctgcat ctacttcttt taaatctaaa gatttaggat
     7741 tggaaacaac atctgacatt gttgctaatg tatcacctga tactttcaat tttagcaagc
     7801 ctgctgattg taacaagtag agagagcgac tttcgttaac tgcatcgttt gggatagtaa
     7861 tagttgcttt atttggaatt tcagaaactg tttggtattt acctttaccg ttcttagtac
     7921 cagagtataa tctaaaggaa gtaaagtatg tctcagcaac ggaaacaaga ttggtcttat
     7981 ttgctttatt ccaattgttt aagtaattat aatgctggaa agcattaata tcaatctcat
     8041 cactttctaa agctttatta ggttgtgtat aatctgtgaa ttccgtaaat tttagcttaa
     8101 cgcctttttt cttaactaat tcttcgattt tatcccaacg agctttttca gttttactct
     8161 ttaccattgt tccaactcta acgactttgg cgtcttcatt tttagactga ccacaggctc
     8221 ctaaaataag tgctgaaatt actactgttg tcgttaaccc taatagcttt ttgattttca
     8281 taatttattt ctcctttgat atggttgtat gattaatcaa ctgatactag tgtaacaaaa
     8341 agctaatgga ttaaaaaata cttattatca atcaagagat atagctttta actatatcat
     8401 ttcttttata attttacttt cgagggctcc gtaaagcggt ccaataatag gcgacttcct
     8461 cataaccttg gtgctcatac atttttctgt tttcttcttc tgaaattaag ataactttct
     8521 taaaccccct acttgcatca aattgtaggc gcgtaccaat tccttgtcct ctgtaggact
     8581 cttggacata aaaatcatct atttccacgt attcaccata gtcccaagct gttacatctc
     8641 caataatttg attatcttta atagctaggt aaactttagc attattattt aaaagattaa
     8701 cacgatt
//
LOCUS       DATRNX010000079         8483 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRNX010000079 DATRNX010000000
VERSION     DATRNX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..677
                     /locus_tag="VBC81_001495"
     CDS             <1..677
                     /locus_tag="VBC81_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6912530.1"
                     /translation="VLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLNDIK
                     IQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLIKP
                     FTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEITYQ
                     HILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYKVY
                     KLIKKT"
     gene            complement(698..1693)
                     /gene="galE"
                     /locus_tag="VBC81_001496"
     CDS             complement(698..1693)
                     /gene="galE"
                     /locus_tag="VBC81_001496"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6912531.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(1781..3388)
                     /locus_tag="VBC81_001497"
     CDS             complement(1781..3388)
                     /locus_tag="VBC81_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6912532.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(3517..4650)
                     /gene="ugpC"
                     /locus_tag="VBC81_001498"
     CDS             complement(3517..4650)
                     /gene="ugpC"
                     /locus_tag="VBC81_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6912533.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(4751..5602)
                     /locus_tag="VBC81_001499"
     CDS             complement(4751..5602)
                     /locus_tag="VBC81_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6912534.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(5697..6593)
                     /locus_tag="VBC81_001500"
     CDS             complement(5697..6593)
                     /locus_tag="VBC81_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6912535.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(6647..7624)
                     /gene="lacD"
                     /locus_tag="VBC81_001501"
     CDS             complement(6647..7624)
                     /gene="lacD"
                     /locus_tag="VBC81_001501"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6912536.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(7626..>8483)
                     /locus_tag="VBC81_001502"
     CDS             complement(7626..>8483)
                     /locus_tag="VBC81_001502"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6912537.1"
                     /translation="NRVTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRS
                     GLDALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDA
                     ATIITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPN
                     LEELTQLIGKPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPK
                     IEVINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQEL
                     ISQIQVKEV"
BASE COUNT         2630 a         1712 c         1388 g         2753 t
ORIGIN      
        1 acgtattaat tatcgaagat gatcctatgg tggaatttat ccaccgcaat taccttgaaa
       61 aactcaatta ttttcaaaac atctattcaa ctgctagtca aacacaagct attgcttatc
      121 ttaatgacat taaaatccaa cttgttcttt tagatattca tattaaagaa ggcaatggtc
      181 tagaattatt aaagttatta cgcaatcaac accaaaatac cgaagtcatt gttatttcag
      241 cagccaacga ggcgcacact gtcaaagaag catttcattt aggtattgta gattacctga
      301 taaaaccatt tacatttgaa cgctttgagt ctagtattga aaaattccta aatcattatc
      361 atacatttga agctgataaa atctatcaag acaacatcga tcactttcaa aaaatagatt
      421 ctggatggct agagggagag gttaaacttg atgagaaagg gctctctgaa attacctatc
      481 agcatattct tgatgccatt caagagttag agcagccttt cactatccaa gagttagcaa
      541 aatgcagtca attttctcat gtctctgtta ggaaatatat tgcctatatg gaagaaaaag
      601 gccttctgac aagtcagcaa atttatacta aggtcggtcg tccttacaaa gtttataagc
      661 ttataaaaaa aacttaggcc tgtagaccta agttttttta atcttcgtag ccatttggat
      721 gactagaatg ccatgcccat gccgatgaaa taattttatc aatattatca aatttaggct
      781 cccacccaag catttgacga gctttctctg aggaagcaat aagcgtatct ggatctcctg
      841 gacgacgagc tgccttttga gcaggaattt ctttcccagt aatacgacga gccgcctcta
      901 acatctgaag gttagaaaaa cctgtcgaag atccgagatt aaacacgttt gattcattcc
      961 cttggcgaag gtaatcaact gctaatatat gagcatctgc caaatcaaat ggatgcacat
     1021 aatctcgaac attagttcca tctggagtat tgtaatcatc tccaaaaatc attattttgt
     1081 cacgtactcc ttgagcaact tgaagaatga ttggtaacaa atgtgtttct ggtttatgat
     1141 cttccccaat tgagccatcc ggtttatctc cagcaacgtt gaagtaacgt agagcgacaa
     1201 acttaatacc gtaggcttga tcagcccatt tcataattgt ttccatcata agcttacttt
     1261 cgccataagg attaatagga ttttgaggag ctgtttctaa aataggaact tgttctggaa
     1321 taccatatgt tgcagctgtt gatgaaaaga caatattttt gatatcacat tcattcatta
     1381 cttctaaaag ttttatcatt ccagcagtgt tattatcaaa atattttaaa ggattttcca
     1441 ttgactcagc aactagtgaa aatgccgcaa aatggataac agcatctaca tcaggatttt
     1501 ccctaaaaac ttgacgcata aacgttttat cggataaatc tccttcataa aaaatcgcat
     1561 ctgaatgcac cgcttgacga tgacctgtta ccaagttatc aacaacaata actttctctt
     1621 ttccttgtgt gattaactga tcaaccatat gcgagccaat gtaaccggcg ccacctaaaa
     1681 tcagaactgc catatttttc tcctatttca atactctttt aacatcattt gttacctcat
     1741 tatactgaga ataagctttt tttgcaaata tctaccgaaa ttagaatagc tctatgcaga
     1801 aggcatccca cggcttgaga gtgttacttg atagtacttc ttgaacctct gtatttgaaa
     1861 taatagtttc tttaacagct tctggaaatt caaaagattg gttttgatcg gataagtttg
     1921 caactattaa ataacgttct ttatctgttt gacgaacata agcaaatacc ttatctgctg
     1981 tttccaaaag tttaaaatca gcatcaacta accagtcgtg ttctttacgt aaagcaacta
     2041 atttttgata agtataaaaa atactttccg tatcatttaa cgctgcctcc acattaattt
     2101 cctgataatt gggattgact gctagccatg gggtaccgtt agtaaaacca gcttgatgtc
     2161 cttgactcca ctgcattgga gtacgagcat tatcacgacc gactttacga atactatcta
     2221 aaattgttgc ttcagatacc ccattatcca tggcttcttt agcatagtta agggattcaa
     2281 tatcatctac atctgctaaa cactcaaatg gataattggt cataccaatt tcttcacctt
     2341 gatagatgta aggcgttcca cgcatcaaat gtagtaggat tgctagagcc ttggcagatt
     2401 gttttctgtt atcactatca tttccccaaa tcgagagcac ccgaggaagg tcgtgattat
     2461 tccagaataa agaattccaa ccttggccaa gctccaattg tgtttgccat tttgtaaaaa
     2521 tatctttcaa tgctggaaca tctaatccgt cactgtaatc ccacttagga gcgtcaggtt
     2581 tatgttgaag acctacatgt tcaaattgaa agaccataga taattcttca ttatcgggat
     2641 ttgagtattg ttttgcaatg tctggcgttg ctccccaagt ttcacccacc gtcaataagt
     2701 catgctttcc aaaactagct ctgttcattt ctttaagata atcatggagt tttggtccat
     2761 taccagtaat gcccttatct ggaattttcc caattaaatc aatgacatcc atacggaaac
     2821 caccaatacc cttatcaatc cagaaattca tcatatcata aattttatga cgtaaagctt
     2881 cattctccca gttgagatct ggctgacgtt tgctaaaaag gtgcaaataa tattgacctg
     2941 atgctttatc atattcccaa gctgagccac taaaaataga agtcaaatcg ttaggttcat
     3001 ctcgccaaat ataaaaatca cgttcaggac tattagggtt ttctcgcgct tctacgaacc
     3061 aagcatgttc atcagaggta tgattcacta ccaaatccat gatgatttta atcccgcgtt
     3121 gatttgcctc ttgtaacagt tggtccatat cattcatgtc accgaaaata tctgcaatgg
     3181 cttgataatc tgaaatatca taaccattgt catccattgg actttggtaa acaggtgata
     3241 accagatagc agtaatccct aatttttcaa ggtagtctag tttgctagta atccctttaa
     3301 tgtcccccac accatcacca tctgaatcca taaatgaacg tgggtaaatt tgataaatcg
     3361 tagctttatg ccaccagtgt ttcttcatta ttcttctcct atcaataatt cattcatctt
     3421 ttattttaac gtttttcctc ttttagtgca agcgtttgcg caaataacat tataaaaagg
     3481 ctggaaccca acctttttat gaatatcgct tataccttaa cgaatagctt gttcagtatc
     3541 tgcatcaaag aaatgcccct tagcaacatt aaacgttagg ttaacttttt ctcctggatt
     3601 atggaagtca cgcgcttcaa cacgagaagc aaattctgtt tgacctaact taacataaag
     3661 catagtttcg ctaccgagta attcagaaac taaaacttct gcttcaacat tggcatttgg
     3721 ataagtatct tggacaagaa gattgcttga aatgtcttct ggacgaatac caaaaatcaa
     3781 ttcttttcct ttatagcctc gtgattctaa aagtttttct tgaccttcag ggatagcgat
     3841 tcgaagacca tcttcactaa taatcatacc attttcaact ttaactttga agaagttcat
     3901 agatggacta ccgatgaatc ccgcaacaaa tttattagca ggaagattat agagttcttg
     3961 tggtgaacca acttgctcaa ttttaccaat tgtaccatca ccatcagggt tcttagtagc
     4021 tgacataatg acaatacggt ctgccaaagt catcgcctct gtttggtcgt gggtaacata
     4081 aatagttgtt gaaccaatac gttgatgaat ttttgcaatt tcagcacgca ttgaaacacg
     4141 taatttagca tctaagttag ataaaggctc atccattaag aacactttgg catcacgcac
     4201 aatagcacgt cccatagcaa cacgttggcg ttgtccacct gacaaatccg caggtttgcg
     4261 ctctaagaat tcagtaaggc caatattagc tgctgcttca cgaacgcgct tatcaatttc
     4321 ttgttttgag aatttacgca atttgagccc aaaagccata ttatcgtaaa cagtcatatg
     4381 tggataaaga gcatagtttt ggaaaaccat agcaatatca cgatctttag gtgatttgtc
     4441 attgacaact tcgccatcaa tcttgagctc accttcacta atatcttcaa gacctgcgat
     4501 catacgaagt gttgttgatt tcccacaacc tgatggtcca acaaaaacaa taaattcttt
     4561 gtctttaata tccaagtcaa agtcttcaac agaataatgc gatgcacttg gatatttttt
     4621 gtaaatatga tttaaattta attctaccat tagattgacc tcttttatta ttgtgatttt
     4681 attatagcaa agaaagggtt ttcatataat ggtaaccttg cctaaatttc ttgtgcaaac
     4741 taactaaatt ttaggtatcc atcgaaatta aataggcata ggctaaatca tctaatattt
     4801 ttaaattaag ccccgaagat tgggtaaatt tatctaattt atattgcaat gaattccgat
     4861 gaatgaaaag tgccttagcc gttttagcaa ggttcccttg ctcttgccac aaggctttta
     4921 ttatagccga agtatcactc gtatcaagga tatgttgtaa acattttgct ttaatagaag
     4981 gcatgggcga ttgatttgcc aaagcccata aagccatctt tggaaatcga cagtaatgtt
     5041 gttcggcctt gtgactaaga aaaagcgtaa aaagtttata ttcttcttca tataattctt
     5101 tccagtccac tgcttgtaga tgcgtccaac tatttccaaa atagcatctt aaacgcgtgt
     5161 taaagtcatt ttcaatcgtt ggtaaaatat ctgttagtaa ttccgtagca ttaaagaaat
     5221 tatcctgatt taaaatcatg acactttggg tttgattaat tgtcataatt gtttctacgt
     5281 ttattactaa aggcgacaag aaatccttaa gttcttctga taaggtaaat tgatgctcaa
     5341 taaaaataaa ttggactgcc gaatgagatt ggggcaacct tccacgacct tcaactagat
     5401 agcggtacca aggattctga gaatccactg aactcgcctc ttccaggctt tgtgtcattt
     5461 gtaataagca tagttcacgc ttagttagcg aagattttgg aaagtggagg acatgaccat
     5521 cttgctcaac gatgatataa tcctcactct gtttaacttc ctgtttagta ataacttctg
     5581 gaaacagttc cttaaaatcc atggcatcct ccactacagt tttgttcatt atatcatatt
     5641 tataacctct ttttgagcac agaaaaagag gagaatgtct cctcagaaag tttctgttat
     5701 aaaatagtga tccgaataga atgctgtttc gactgctttg gattaagccg tacgatattt
     5761 tgtttatctt ctaaaatatc agactcttca atagaagttg acaaggaact ccagggttcc
     5821 aaagccaaaa aaggaccacc attgttactt gtccaaagaa taagattctc aaaatcatca
     5881 aagtcaagtt ggattccttt tgcatgattt ctagatttta agtagaccgt cttagatcgt
     5941 agttggtcta aagtaatagc atctttttca aataaagcat aattaagaga taattgtttt
     6001 tgattttcta aaaaaggatg tctagcttgt aaatctaaca agcctgtatc cggaaacgac
     6061 tcaggaattg tgcaagtttc aaactgttca aactctagat agtaatcact aaatacttcc
     6121 ttttcaaaca aagggcaatt aaagcctggg tgagctccga tagcatatgg catatttttt
     6181 tctgattcta agtttttgac aatatatgta atggcaatgc ttttatttct taaggtgtaa
     6241 gttatttcta aactaaaacg ataaggataa tttatcaacg tttcttcact acttttaatt
     6301 gtaaaacgaa cagaagaatc cgatatgtaa tcatagtcga atgttttgtg acgtacaaag
     6361 ccatgccttg gtatgatacc cgtaaaatgt ggagcttgag ctggtctgta aatgacgcaa
     6421 tcatttcgaa cacttccgca tataggaaat aaaataggag cttgcccacc ccaataagtg
     6481 ggttcccctt gccaaaggta ctcaacgcct tgactatctg taatagaagt cattgttgca
     6541 cctaacgctt ttaatgtcac ttgcaactca tgattttgta tcgtaatagc cattattttt
     6601 cctttcataa agagcaataa ttgctttacc aacttttctc tagcaattat ctttccttcc
     6661 aagaagttgc tgttttttgc aaaattttat ttagttcatc aatattttga aaacctattg
     6721 tacgtaacca ttgacgtgca ccagcttctc ctttttcaac atactcttta acacttccag
     6781 cccaagttgc tctaccacaa aggacaccgt taaatttagc accagcttct ttagcaaaca
     6841 caagagtctc ttgaaaaagc tgagcaggaa ctcctgcact caaaaaaatg taggggagta
     6901 gtgtcgcctg atcttgttct ctaaaataag ccgcagctgt cgctttatta taagcagtct
     6961 ctccttgcgc aaaaccttct acatagtcca tattgacagg cacttccacc ttcaagacat
     7021 caatattaaa tcgaggattt gaaaaaactt tcatagcttc aataacctta cgtggcttaa
     7081 ttttggcata ctcaatacct gagctatccg taatcttttc atcatatgac aaaatttcaa
     7141 ggaaaaaagg aatgtcttct gcaacacatt cagaaccaat ccgttcaata taagcttctt
     7201 tttcttcgtt tacttcatcc gaactatcaa catcataata aagtaagaac ttaactgcgt
     7261 ctgccccctc ttctttaatg cgtttcgcag accaaatgtc taaacagtct gggagtcgct
     7321 tggtactact ggtatcataa cctgtttttt cgtacgcaag taaaagaccg gcattgtcat
     7381 ctaaaacttt cgtagctggc aacccatatt ctggatctaa caacattgat gaagcgtaag
     7441 gggttaattc ttcagctacc aaaactttaa gtgcttctat ttgagaaaca gtgggctcct
     7501 cagattgata ttttgccatt aatcgcttca atgctccacg ttggtcaaaa gccaaggccg
     7561 aaataatacc atttttatca ctcaattgtt ccatatgttt ttgtttttgt tccgttaatc
     7621 ccatcctata cctctttcac ttggatttga ctgattaatt cttgatagta ggtcagattg
     7681 atatgccctg ttaatgtttc ttgtgcatta agcataccca agacattagc tctcttaagt
     7741 aaatttatat catctagttg atgttctaaa gcagacgcga ttcctgcaac cgttgcatcc
     7801 cctgaaccaa ctggattgat aacttctatt tttggaatcg tcacttgata gtagtgattc
     7861 ccatgtttag caaaagctcc cctagcacct aaggaaacaa taacccaatc aattcctcta
     7921 aaaatatctt gctgtaaggt agattttaat tcgtctaaac tatagctaat tggctttcca
     7981 attaattgtg ttaattcttc aaggttaggt ttaatgacgg tcggcttagc actagatttt
     8041 aaaaccgaac gtaatgcttc tcctgaacag tctaaaacaa ctttcttatt aaagtgattc
     8101 gctagggaga ttaacctagc ataataatct gaggggagtc cctttggtaa acttcctgat
     8161 actgttatga tagttgcagc atcaaagata agttttagat gattcaaaaa ggcgcctgct
     8221 tcatctttag taatatgtgg acctttttct agaatttcag tctgctgacc ctcatgtaaa
     8281 actgcaatac aatgacgtgt ttctccacca atacttaaaa attggtgtct gatttccaat
     8341 gcatctaagc cacttcggat gaaatctcca aaatgccccc ctaatagacc agttgccact
     8401 acattatcgc caagttgaga aagaacacga gtcacattta atcccttacc accaggtgtt
     8461 ttggagacgt cggtaacccg att
//
LOCUS       DATRNX010000080         8291 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRNX010000080 DATRNX010000000
VERSION     DATRNX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8291
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            162..866
                     /locus_tag="VBC81_001503"
     CDS             162..866
                     /locus_tag="VBC81_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6912538.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            1132..2364
                     /gene="arcA"
                     /locus_tag="VBC81_001504"
     CDS             1132..2364
                     /gene="arcA"
                     /locus_tag="VBC81_001504"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6912539.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            2370..2800
                     /locus_tag="VBC81_001505"
                     /pseudo
     CDS             2370..2800
                     /locus_tag="VBC81_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2816..3829
                     /gene="argF"
                     /locus_tag="VBC81_001506"
     CDS             2816..3829
                     /gene="argF"
                     /locus_tag="VBC81_001506"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6912540.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3892..5319
                     /gene="arcD"
                     /locus_tag="VBC81_001507"
     CDS             3892..5319
                     /gene="arcD"
                     /locus_tag="VBC81_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6912541.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            5340..6296
                     /gene="arcC"
                     /locus_tag="VBC81_001508"
     CDS             5340..6296
                     /gene="arcC"
                     /locus_tag="VBC81_001508"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6912542.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
     gene            complement(6416..7441)
                     /gene="trpS"
                     /locus_tag="VBC81_001509"
     CDS             complement(6416..7441)
                     /gene="trpS"
                     /locus_tag="VBC81_001509"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6912543.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(7549..8241)
                     /locus_tag="VBC81_001510"
     CDS             complement(7549..8241)
                     /locus_tag="VBC81_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6912544.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
BASE COUNT         2750 a         1443 c         1453 g         2645 t
ORIGIN      
        1 ataatgaaaa ttttttaatg tataaaattt tcttgtcacg ataaatcctc cttcattata
       61 gcaaaaaaat tcgtagaaag aacgtttttc gttgaatttt gaaaacgctt ttgagaaaat
      121 gtaattgttc tgatatcttt taaaaaaatt aagaggcctt catgactaaa tttattgttg
      181 attcatcgta ctggaatttg tttccaactt ctaaaattgg agtaatctta atcaaagatt
      241 atcacatgga caggaacctt gaaacagaac ttaaacagtt actgtctgat agtcattctt
      301 tagcaaaaaa atatttgcaa gagaaagaat tcagtcaaaa caaagttatc caaacttatc
      361 gcaaagcata tcaaaccttt aaaactaaaa aaggtgcacg ttcaagtatc gaagcattat
      421 tgaagcgaat taattcagga aacgaaatta ctagtataaa tcctctagtt gatatctata
      481 atgccgctag ccttaggttt ggattaccta ttggtgctga ggatagtgat acatttagag
      541 gtgatttaaa actaactatt accaatggtg gtgatgaatt ttacttgatt ggcgaagatt
      601 ttaacatacc aactctttca ggtgagcttg cctatgttga tgatgttgga gcagtttgcc
      661 gctgttttaa ttggcgtgat ggaaaacgta ctatgattac cgataatact cagaacgctt
      721 ttttagttat cgaattaatt gataatagaa gagaaattat ttttaaagaa gcgttggact
      781 ttatcgccac caatacaaat cgctttctaa aagctaaaac acagaccatc atattagata
      841 aagaacacag tgaaataacg ctataacatt aaaacgaaaa gtaaaaattt tacttttcgt
      901 tattttttag gttaataatt tctcactttc caacgataaa agaaagaaat tgtgtagaaa
      961 tcgctttcat ttttataaat ttaaattttt ttggatataa tcgtgacatt cttaacactt
     1021 ttaatgcgaa taacttccct attttatatt caaaagaaag tagaatgtaa gtgtagatat
     1081 agagataaat cctgtttcaa tgcaaatctc taactaagaa aggagtaaaa tatgactcaa
     1141 acacatccaa ttcatgtttt ctcagaaatt ggaaaactta aaaaagttat gctacaccgt
     1201 ccaggtaaag aaatagaaaa tctgatgcct gattatttag agagactctt gtttgatgat
     1261 atcccatttt tggaagatgc acaaaaagaa cacgatgcgt ttgcacaagc acttcgcaac
     1321 gaaggagtcg aagtacttta ccttgaaaat cttgctgccg agtcgctaac aaaccaagaa
     1381 attcgtgaac agtttatcga tgagtacatt ggcgaagcaa atgttcgcgg tcgtgctact
     1441 aaaaaagcta ttcgagaatt attactcaat atcaaagata ataaagaact tatcgaaaaa
     1501 actatggcag gtatccaaaa gtctgagcta cctgaaatcc catcttctga aaaaggtcta
     1561 actgatcttg tcgaatctaa ttatccattt gccatagatc cgatgccaaa tctttacttt
     1621 acacgtgatc catttgcaac tattgggaat ggagtttcat taaaccatat gttctctgaa
     1681 acacgtaatc gcgaaacttt atacggtaaa tatatcttta cacatcaccc agagtacggt
     1741 ggaaaagtcc ctatggtcta tgatcgtgaa gaaacaactc gtattgaagg tggagatgag
     1801 ctcgtccttt ctaaagatgt ccttgccgtg ggtatctctc aacgtactga tgctgcctct
     1861 attgaaaaac tcttagtcaa tatttttaaa caaaatttag gttttaaaaa ggttttagca
     1921 tttgaatttg caaataatcg taaatttatg caccttgaca cagtctttac aatggttgac
     1981 tatgacaagt ttacaattca cccagaaatt gaaggcgatc ttcgcgttta ctctgtaact
     2041 tacgaaaacc aagatcttca tattgaagaa gaaaaaggtg atttggcaga tcttctagcg
     2101 aaaaacctag gagttgagaa agtggaattg attcgttgcg ggggtgacaa cttagtcgct
     2161 gctggacgtg aacaatggaa tgatggttca aatacattaa ctatagcacc aggtgtcgtt
     2221 gtcgtttaca acagaaatac aatcacaaat gcaatcttag agtccaaagg attgaaatta
     2281 attaagatta atggaagtga attagttcgt gggcgtggtg gaccacgctg catgtcaatg
     2341 ccatttgaac gtgaagacct ttaaaattta tgaagctatt agtcaatcac ttgccttacc
     2401 aaagagctgc aagcctatat attcgctttt ctgttttcgt tatcgaaaga aatataaaaa
     2461 tgaaagaaga attcgatgat aatgacgagc aagatactat ttatgctgtt ctttatgatg
     2521 gaaacagcca gtttcaacag gacgctttct tcctgaaact caaacagaag ctcgtttgac
     2581 tcgaattgcg actttaaaag gttatcgtgg taacggctat ggtaccaaaa ttattatagc
     2641 actagaaaat tatgcaaaag aaaatggtta tcactatcta actatacatg ctgaattgac
     2701 agcaaaagat ttctaccaga cacttgggta tcaagctact gggaatattt atatggaaga
     2761 cggggaagcc tgtcaaacac tcgaaaaata tttaatttaa aggagacatt aacatatgac
     2821 acaagtattt caaggacgta gtttcttagc agaaaaagat ttttctcgtg aggaatttga
     2881 atatcttatt gatttttcag ctcatttaaa agaccttaaa aaacgtggtg ttcctcatca
     2941 ttatcttgaa ggtaaaaata ttgctctctt atttgaaaaa acatctactc gtactcgcgc
     3001 agcctttaca actgcagcaa ttgacctagg cgctcatccg gaataccttg gtgcaaatga
     3061 tattcaactt ggtaaaaaag aatcaacaga agatactgct aaggttttag gacgtatgtt
     3121 tgatggtatt gaattccgtg gttttagcca aagaatggtt gaagagcttg ctgaattttc
     3181 tggagtacct gtctggaatg gtttaacaga tgaatggcat ccaacacaaa tgctagctga
     3241 ctaccttact atcaaagaaa acttcggtaa acttgaaggt attactcttg tttactgtgg
     3301 tgacggacgt aacaatgttg ccaactcgct tttagtggct gggactttga tgggggtcaa
     3361 tgtacacatc ttttctccaa aagaactttt ccctgctgaa gagattgtta aattggctga
     3421 aggatatgcc aaagaatctg gggctcacgt tctcgttact gataatgtag acgaagctgt
     3481 aaagggagca gacgtctttt acactgatgt ctgggtatcg atgggagaag aagataagtt
     3541 caaagaacgc gttgaacttc ttcaaccata tcaagtaaac atggaactga ttaaaaaagc
     3601 taataatgat aatcttatct tcttacactg cttacctgca ttccatgata caaataccgt
     3661 ttatggcaaa gacgtcgctg aaaaatttgg ggtcaaggaa atggaagtta ctgatgaagt
     3721 cttccgtagc aaatatgctc gtcatttcga ccaagctgaa aatcgtatgc acactattaa
     3781 agctgtaatg gctgcaaccc ttggaaatct tttcattcca aaagtttaaa ttaaacgact
     3841 atagggagtt gacatatagt caattcccta ttttaggaaa tgaggtataa aatggaaaaa
     3901 gaaaaaaaat taggtctttt accactaaca atgcttgtca ttggctctct tatcggtggc
     3961 ggaatctttg atttaatgca aaatatgagt tccagagccg gtttggtacc aatgcttatt
     4021 gcttgggtaa ttactgctat cgggatggga actttcgttt taagttttca aaatttatct
     4081 gaaaaaaggc cggacctaac agctggaatc tttagttacg ctaaagaggg gtttggaaac
     4141 tttatgggat ttaactctgc atggggttat tggttatcag cttggcttgg aaatgttgcc
     4201 tacgctgcac tcttattcag ttcactcggt tatttcttta aattctttgg taatggaaat
     4261 aatatcatct caattattgg agcaagtata gttatttggg ttgtccattt cttaatttta
     4321 agaggtgtta atacagctgc atttattaat accgtagtta cctttgcaaa attagtacct
     4381 gttattattt tcttaatttc agcgttatta gctttcaaat ttaacatttt tagtcttgat
     4441 atctggggaa atggattaca tcaatcaatt ttcaaccaag tcaattcaac tatgaaaacc
     4501 gctgtttggg tatttattgg tattgagggc gccgttgtct tctcaggtcg tgctaaaaaa
     4561 cactctgata ttggtaaagc aagtatccta gcattattca ctatgatttc actttatgta
     4621 ttgatttctg ttttatcact tggtatcatg tcacgtccag aacttgcaaa cttaaaaaca
     4681 ccagctatgg cttacgttct agaaaaagct gttggtcact ggggtgctat cttagttaac
     4741 cttggtgtta tcatttcagt atttggcgct attcttgctt ggactttatt tgcagcagaa
     4801 ttaccatatc aagctgctaa agaaggtgct tttcctaaat tttttgcaaa agaaaataaa
     4861 aacaaagctc caatcaactc actcttagtc actaatcttt gtgtacaagc attcttaatc
     4921 acgttcttat tcacacaaag tgcttatcgt tttggtttcg cattagcatc atctgctatc
     4981 ttaattcctt atgcttttac agcactatat caattacaat tcacactccg tgaggataag
     5041 tcaactccag gacatcaaaa gaatttaatt atcggtatcc tcgctacaat ctatgctgtt
     5101 taccttatct acgctggtgg ttttgattac ttacttttga caatgattgc ttatactcta
     5161 ggtatgattc tctatattaa aatgagaaaa gatgacaagc ttcctatctt tgtgagatat
     5221 gaaaaaatta gtgccattgt cattctagcg ctttgcttac tatgtattat tgaaattatg
     5281 actggacaaa ttgacattgc tgcaatccta gcaggttagt cattagaaag agggataaat
     5341 tggtaaaaga acatcaaaaa atcgtagttg ccttaggtgg taacgctatt ctatcaacag
     5401 atgcttcagc aaaagcacaa caagaagcat taatcaacac atcaaaatca ctggtcaaac
     5461 tgattaaaga aggccatgat gtcattgtta ctcatggaaa tggtccccaa gttggtaact
     5521 tattgctaca gcaagcagat tcagattctg aaaagaatcc agcaatgcct ttggatactt
     5581 gtgttgccat gacagaagga agtattggtt tttggttaca aaatgctctt aacaatgaat
     5641 tacaagaaca aggcattgac aaagaggttg caacagtggt aactcaagtt attgtagatg
     5701 aaaaagatca agctttcact aatccaacaa aacctattgg cccattctta tctgaagaag
     5761 atgccaagaa acaagcccaa gaaacaggtt caaaatttaa agaagatgct ggtcgtggat
     5821 ggcgtaaagt cgttccttca ccaaaaccgg ttggtatcaa agaagcttct gttattagac
     5881 ggttagtgga ctctggtgtg gttgttatta gcgcaggtgg cggaggtgta ccagttattg
     5941 aagatgctaa tacaaaagca ttaaaagggg ttgaagctgt tattgacaaa gactttgcca
     6001 gtcaaacttt atcagaactt gttgatgccg acttattcat cgtcttaact ggcgttgaca
     6061 acgtttttgt taattttaat aaacctaatc aagaaaaatt agaggaagtt actgtttcac
     6121 aaatgaaaca atatatcact gaaaatcaat ttgcccctgg cagtatgcta ccaaaggtag
     6181 aagctgcgat tgcttttgtt gaaaataaac cagaagcgcg tgctatcatt acttcacttg
     6241 aaaatattga taatgtctta gcagaaaatg cagggacaca aattgttgta aattaaacaa
     6301 tgtagattac cattacatac actccttatt aataacaaag taatgtcact ccttaggcat
     6361 tcctataata aaagagagct agatatatct agctctcttt tattatagga atatattatt
     6421 taaaatagtt caaacccatg gctgatttaa cctcatccaa agtagaggct gcaacagctt
     6481 gagctttctc actacctttt tgtaacattt gataaacttg tcccatatct ttagcatatt
     6541 caaggcgacg ttctctaata ggtgataatt cacgctctaa gatatcaagt agataacgtt
     6601 ttgttttaac atctccaagt ccaccttttt gatagtgttc tttcatagca gtaatctctt
     6661 tttggtcttc atctcttcca aaaacatcaa ggtaatggaa taccatatta ccttcgattt
     6721 gtcctggttc ttctacttta atatggtttg gatcagtata catgctcata acttttttct
     6781 taactgtatc catatcatct gctaaaaaga ttccattatt taatgactta gacatttttg
     6841 cattaccatc taaccctggc agacgacctg ctgcatcatt ttctggatag attccctctg
     6901 gttcaactaa aacctgacaa ttataagcat gattgaaaga tctaactatt tcacgtgttt
     6961 gttcaatcat tggtttttgg tcagtcccta caggaacaag gttagcttta aaagcggtta
     7021 tatcagctgc ttgtgcaaca ggataaacta agaatcctgc tggaatagac tcaccaaaac
     7081 ctttttgagc aatttctgtt tttacagttg gattacgttc caaacgtgca agtgaaacca
     7141 agttcatata atacatactt aattctgcta actcaggaat ttgactttga atgaataaag
     7201 tcgacttgtt tggatccagc ccaacagcta aataatcaag tgcaacatta ccaattgatt
     7261 caacaatagt ttgcggatct ttagcatggt ctgtcagtgc ttgttggtct gctaaaaaga
     7321 caaataaagt atagctaccc tcattttgaa gtagaactct attttttaag ctacctacat
     7381 aatgtccaat atgcaacttt cctgtcggtc tatcacctgt taagataatt ggttttgtca
     7441 tgtttactcc ttaatatacc aaagatatca tttcatatag ttatcttatc atgtcacaag
     7501 ccctattgca attcatccct acaagaaaca ttcaccttat taagatgatt aaacattatg
     7561 taacgttaaa tcgttcttaa taatttcaat cgtataatga atcataataa ttaaaagcgt
     7621 tatcgtcaca cctaaaatag ctctatatgc aaagatatcg taaactattg cagctaccaa
     7681 tgctccaata ataaatgcta gaactaccag tgaataattg atagctcgtc ttttttgcat
     7741 agccgttctc tgacgattaa caatatactc ataccaaaat tgtagcattt tccgataatt
     7801 accagaagtc attaagatag tgtatggttc agattcaatg cgacttcctg aaaatgtcaa
     7861 cattaaaata cctgtgctaa aggctaataa agctacccag atattgtggt ttacattttc
     7921 aacaaaagga ataaacatcg tagaaagtaa aaggggacat aatgcatata gacgccaaaa
     7981 agctgttact gcacgttctt taatcacaat accaaccaaa aaaccaattg aaaaaaagaa
     8041 aatagaaaat aatttagtga taaaaaagtg aaaatcattt tgtccgagat ttgccaccat
     8101 caagataata ttacctgtct gtgttgcaac aagggagtga aactgcatgt gactataaac
     8161 gtcaataaaa ccaccaataa aacccaataa tacagccatt tttcttccat cttgtggcat
     8221 tataatatca tctctgcgca ttatagcaaa ttctccttct tgtattatca tgttttctat
     8281 tatataccaa t
//
LOCUS       DATRNX010000081         8200 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRNX010000081 DATRNX010000000
VERSION     DATRNX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(13..366)
                     /locus_tag="VBC81_001511"
     CDS             complement(13..366)
                     /locus_tag="VBC81_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6912545.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            complement(363..1094)
                     /locus_tag="VBC81_001512"
     CDS             complement(363..1094)
                     /locus_tag="VBC81_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6912546.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            complement(1096..2037)
                     /locus_tag="VBC81_001513"
     CDS             complement(1096..2037)
                     /locus_tag="VBC81_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6912547.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            complement(2027..3181)
                     /locus_tag="VBC81_001514"
     CDS             complement(2027..3181)
                     /locus_tag="VBC81_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6912548.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            complement(3298..4152)
                     /gene="rfbD"
                     /locus_tag="VBC81_001515"
     CDS             complement(3298..4152)
                     /gene="rfbD"
                     /locus_tag="VBC81_001515"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6912549.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            complement(4242..4583)
                     /locus_tag="VBC81_001516"
     CDS             complement(4242..4583)
                     /locus_tag="VBC81_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6912550.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            complement(4692..5801)
                     /gene="rpoD"
                     /locus_tag="VBC81_001517"
     CDS             complement(4692..5801)
                     /gene="rpoD"
                     /locus_tag="VBC81_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6912551.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            complement(5809..7683)
                     /gene="dnaG"
                     /locus_tag="VBC81_001518"
     CDS             complement(5809..7683)
                     /gene="dnaG"
                     /locus_tag="VBC81_001518"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6912552.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            7807..8184
                     /gene="mscL"
                     /locus_tag="VBC81_001519"
     CDS             7807..8184
                     /gene="mscL"
                     /locus_tag="VBC81_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6912553.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT         2571 a         1635 c         1230 g         2764 t
ORIGIN      
        1 aaaatatgtt ttttactcat ctaaagtgct atcctctatc tcttttttca acaaagaaac
       61 ctcttgaatc aggcgtttaa tttgctcatc tcttttggta ttgctactag tatcaaataa
      121 ttctaaacaa aacagaaata aaatagcacc aaataacaag aaattcatcg ttaaagcgaa
      181 tccaaggtga tgagcgataa atgagaccac cccaggaaat acactaataa agactagaat
      241 taaaccaact aatagccaag gtaaagaacg tttaacagaa aatgtgtttt tattaaccat
      301 tcttataatc atataaagta ggacaatggc taaaataaac atttcaattt gcaatgctaa
      361 actcatttat catcctcctt aataaaccct gcaataataa ttgatgttaa aacatccgtc
      421 atataaaaaa tcgattttag ggaacttata cttgaagtac ctgcctcacg ttcaaacata
      481 ttagctgtga cttccttcaa acgatagcct tttttcaaaa tccgagcagt tgattcaggc
      541 tctgggtatt gtactggata atgacacgat aaataagcta ttagcgccgc gttggctgcc
      601 ctataaccac ttgttgtatc atagactttg tgtcccgtcg ttaattttat agcagcagat
      661 attaaattta tgccaagacg cctcattgct gtcgattgaa aattttgatg ttttttatca
      721 acaaagcgag aaccaataac aaaatctgct tcgtcattta agattggcat aacaacatct
      781 tcaatgcttc taatatcatg ttgaccatca ccatcaaatt gaacggcaac atcgtaatga
      841 ttacgattag cgtataagta tcctgtctgc acacagccac caatacctag attttgaaca
      901 aggtcaatat gatttaaacc caatctatct aataactctg gagtaccatc ggttgaccca
      961 tcgttaataa caatataatc cagctcaaaa ggtagtgagc gagacgcctt aaaatctaca
     1021 atagtctgga ctgtctttgc aatactacct tcttcattat aagcaggtat cactagtaaa
     1081 atttttttca tcatcttatc tcttattgta ataatttgtt gcaatcaacc atttaaacac
     1141 aacttgatgc ttaaattgat ttttgctata accatatttc cataaccacc ttaatcttgt
     1201 cataaaaggc tgtttgtcaa tttttataaa acatttaatg agctcaagat cattagctgt
     1261 cataatatca ccatacttat caacaattaa ggaagcttgc ttttgtgagt catggataag
     1321 tttccaatac cttgtaaata tagatttagg accttcacga agtatcttaa atctcttatc
     1381 cattgtcctc gcccccaaaa cattattgtc atgttgacgg tatagttgag ttggtaaatc
     1441 caaataaatg atttctccta aacttgctgc taagagtgct aaaaaccaat catgcattaa
     1501 gatatcatta ggagtaaacc acttctcagc taatgcatga tttatcatca tagtcccacc
     1561 agtaacagta ttctcagtta gttctggtaa taatgtcgta ttggcatggt gcgattgagc
     1621 ccgaatcatg ctgtcttgta aaatatttaa ttcttgatta acaactttca aatcagtata
     1681 aacaagtaat ggtacatcag atgctttatg ctttgctgcc tctaaactta cagataactt
     1741 ttctggcaac catacatcat cttgatcact aaaaaaatag aaatctgcaa cttcgtaatt
     1801 aactagcgta aaaaatgatt taataactcc taaattatgg tgctcatttt cattaatgaa
     1861 acgaattcga gaatctttag cagtaaagtt tctaataata tcacatgtct tgtcacttga
     1921 accatcatct ctgattagga ggttccactc tttaaaggtt tgcttttgga tactttcgat
     1981 ttgctgcgcc agaaacttct ctccattgta ggtggccatt aaaatattaa ctttcattta
     2041 aaaacaaatc ctcatattct tcaacaattt ttttccaagt atagttttct tgaataatgg
     2101 cttttgctcg atcaccgtaa gtagataaat cctgttgttg atcactatcg ttaataagct
     2161 tagctaaatc cccctctttt ttagtccagt aaaaacttga gagacctgct actgatttgt
     2221 tgaaatcaac atctagaaca agattcaaat cagtattagc taaagcctca agcagtcctg
     2281 gattagtacc gccaacttca tgcccatgaa tataagcaaa ggcttgttga cgaacataat
     2341 ccagcagatc tttttcatag agcgtaccta caaacttaac tcttttatct tgttgaaggt
     2401 ttgtctttaa ggacaacttt tcaaagtagg ggttattttg atggttacag ataataacta
     2461 aatcacgctt agtatctgaa gccatgaact ccctaatagc cgtttcataa ttgttttcag
     2521 ggacaaatcg accaacgatt agatagtaac ccttagactt aatattccat tttttaaaca
     2581 actgtttgac acgtggatca tctgacgata atttcctaga attaatctct gtaccgtaag
     2641 caatgaacct tgtcttcaca ttagagtagg tagattgaat gtaattttca ataccaatat
     2701 tatcagaaat aattaggtct gcatttttag tcatacattt ttcggcgtat tttaaataac
     2761 gctgtgtggg acgagaccac tttgatcgct tccactctaa accatccgga ttaacataca
     2821 ataagccacc gactttatgt attttattgg caaaatgcca aataaaggca ccaattgtat
     2881 ttcctaaaat ataaaaaata ggctctttta aatcatgtgt cttaaccaag tcaagggcat
     2941 aattaatggc cataatatca taagcaatca cacgtgctgg ccctaattgg ggaggattta
     3001 tagtaaaaca atcagcgtca gcaaagttaa aatgagtatg atgttcttta tcactaaggc
     3061 atgcaacatg gtattttatg tcggaacttt tttgatgatt aatcaattct gaaacaaaag
     3121 tttcaaaacc accgtaacga gctggcaacc ctctacttcc tataatgaaa acatcttgca
     3181 tcatcttctc cattctagct aaacaatcca tacaattata ccataaatta gaaaagttag
     3241 ctaaaaagag cagtaagcta aaaggattct ctagccttac tactctttgt tttgatctta
     3301 ttttttaact tcctgcttgt agaactcttt taaagcatct tgccaagtag gaatcacaaa
     3361 tcctgttgct ttcgcttttt ccaaactcat agttgaattt aaaggacgtt ttgcttttgc
     3421 tgggaattga ctagaatcaa caggcttaac ctccacatct gtgtctttta agatttcaac
     3481 cgcaaaatca taccatgttg tatcttcttt agcatcattt gataaatgat agtaaccaaa
     3541 atctttttga ttttcagcta aataagtcat aaactcagcc aaagtacgtg tccatgttgg
     3601 tcgtccatgt tggtcgttaa caacagttag agttttgtgt gtttttgcaa ggttttgcat
     3661 cgtaaagaca aagtttttac catagttacc aaatacccac gctgtgcgaa tggtatagaa
     3721 ttttgaagta tatttttcaa ctaactcttc acccatacgt tttgtacgac cgtactctgt
     3781 tttgggatca ggcagatcat ctacttccca ttcttgcccc actggttttt caccatcaaa
     3841 cacgtagtcc gtcgaaatgt aaacaagtgt tgcatcatgt ttagcagcag ccttagcgac
     3901 attctctgta ccagtgacat tgattgcaaa gtctagctct tttccttcat cttctgccgc
     3961 atcaacagct gtgtaagctg cacaatggta aactaaagat ggcttcacct cttcaaagac
     4021 tttgtccacc atctcggcat ttgtaatatc catttcagct acatcaacag caacatactc
     4081 ttgagttcgt tcgtctaaaa ggtgacgtaa ttcactacct aattgcccgt ttgcccctgt
     4141 aattaaaatc attccttgct cctttaaaaa taagtttaac ttttaattct tcgtttattg
     4201 tacactaaaa aagggaatat ttcatccctt tactctttat cttatgaacg aattcccaaa
     4261 gcgatacgag cataacgact catcttatca acagaccagg ctgggtacca taccaatttc
     4321 acctcggttt cagttacctc tgggactgtt ttcataacat catgaatttg atctgtcaat
     4381 aaatctgcta acgggcatcc catagtcgtt aaagtcatat caatttcagt tcgaccgtta
     4441 tcttcaaagc gaatctcata aattaaccca aggttaacaa tatctattcc caattcagga
     4501 tcgataacca tctccaaagc ttctaggata cgatctttta tttttccaac ttcttcttcg
     4561 ctatattgct ttacttctga cattataata cctctctgaa aatattaata ccgtctttaa
     4621 gataactcct ttgcaaatca tttttattag aaactaaact tcctattcta aaaacgatta
     4681 tcgagaagca atcaatcttc catgaaatct ttaagttgct tgctacgact aggatggcga
     4741 agttttctaa gggctttagc ttcaatctga cgaatgcgct cacgagtaac attaaatact
     4801 ttacctacgt cctctaacgt acgcatttta ccatcgtcaa gaccaaagcg taaacgaaga
     4861 acattctctt cacggtcagt aagagtatct aaaacctcat ctaattgctc acgaagaaca
     4921 acacgtgtag tataatctac tggattttca ataacttcgt cttcaatgaa atcacccaaa
     4981 tggctatcat cttcctcacc gattggcgtt tctagcgata ccggctcttg agcaattttt
     5041 aggatttcac gtactttatc tggtgtcata tccatgcgtt ctgcgatttg ctcaggagtt
     5101 ggatcttgac caagttcttg aagaagatta cgttgttcac ggaccaattt attaatcgtt
     5161 tcaaccatat gaacaggaat acgaatcgta cgtgcttgat ctgcaattgc acgtgttata
     5221 gcttgacgaa tccaccatgt tgcataagtt gaaaatttga atccctttga ataatcaaat
     5281 ttatcaacag ctttcatgag ccccatgtta ccctcttgaa ttaaatcaag aaattgcatt
     5341 ccacgtccaa cataacgttt tgcaattgag acaacaagac gaaggtttgc ttcagctaaa
     5401 cgttgtttag ccattaaatc tccctcagca accgcaacag ccaattcttt ttcttcttca
     5461 ttcgttaaaa gaggaacaac accaatttcc ttcaagtaca tacgtactgg gtcattgact
     5521 ttagcagaat tacttcctat gagctcttca tcagtcaatt cttcaggttt tggcccttct
     5581 accacatact ttgttgaagg attgccttct ttatctgtga ttgaaatgcc accgtctgtc
     5641 aatcgttcta acaaatcatc aatttgatct gcatctaaaa caaaaggaat aacaagtttt
     5701 tctgttacct catcatcaat tgcagtacct tgttttttat gattgcgaat aaaatccgca
     5761 acttgaacat taaaagttgt atttcctttt ttctctgcca taggttcctc attccatatt
     5821 tcttttttga gcaattaaat tttctaatgc agccaaggct ccttcttcat caccttgatt
     5881 actagattgt ctaattaact ggctttgttt tcttaaatca cgttctttca ataaacggtc
     5941 acgtgccttt tcgacggctt caatctctcc aatagatact tctacaggta attgttcctc
     6001 taaaattata taataagtac gattaacttt gtcactttct tgtgataaat cataagacgt
     6061 gatctcacca ctttctttta gcaactcata taaaacttgt atttctgttg tatcaaaata
     6121 aaaattatcc ctattacgga attcatttaa caggtaggga tgattcaata atcgatgaaa
     6181 caattgcatt tctgttttag tgatagctga taacttacct gtctgtggta attgcactgt
     6241 agcacttgtt tgtactgaag atgaagcttg ctgttgactt ctatgatgca atcgttcgtt
     6301 attaaccgat tgttctactt gaaaataatc aaaatctggc aagagttcag ccacttttgt
     6361 aatatatgaa ttttgtgctg ttatcgatgg agatttagca attaatttcg ctattttttc
     6421 aacataagca atttcagatt gtaaattatc ggtgttttca ggctttaagt agtggatata
     6481 aaactctgta ttactgatgc gcccattttc taagagttgt ttaaaatctt ctgctgaatt
     6541 tgcttgtaga aattcatcag ggtccatttt attaggtatc ctaacgatat caacagtcat
     6601 atctgatagc agttctaaag acttgtcaat tgcgttttga ccagctctat caccatcata
     6661 agtcagaaca actttcttag taaatctttt aaggtgtcta acgtgttcat tggttaaagc
     6721 ggtccccata gaagcaacaa cattctcaat tcctgcacga taagctgcaa taacatccat
     6781 aaagccttcc attaggtaaa cttcgtgtgc tttattaatt acagcacgcg ctttatctaa
     6841 atggtaaagc tcgtaagact tattaaaaat aggggtggca cgagaatttt tatatttagc
     6901 ttgttttcta tcaatgtcct cttgagtcca aactcgtcct gaaaacgcaa tgacttcacc
     6961 aatatcatta gttaacggaa acataattct attattaaat gaatcgtaaa actgattgct
     7021 attttctgaa taattgaaca aaccagaaga catcaaagtt ttttcatcaa actcttttgc
     7081 cagacgacga tatagaaaat cctgccctcc cggcgataat cctatttgaa aatgtttaat
     7141 taagtcctct gtcactccgc gctcggcaag gtattgtcta gcgcgttcac catcattagt
     7201 tgtcattaaa taggcatggt aaaatcccga agcaactcta tgaatatcta aaagcgactg
     7261 attagcttta ggttttttag gaacttgccc cttaaaatta gtatctagag gtattcctga
     7321 acgttcagct agaacttgta cactatctaa aaaactaata tgttggtaat cttccacaaa
     7381 tttaaagaca tcaccagagc gaccacaacc aaaacaatga aaaaactgcc tatcttcgat
     7441 aacattaaaa gatggcgttt tttctttatg aaatgggcat aaaccaagat gattgcgtcc
     7501 tgtttttgtc aaaccgacaa cttcaccgat gacatcaaca atgttaacac tgttcttgat
     7561 ttcagaaatt ttttctttat caattgccaa gtcatgacct ccacagaaat aacccattat
     7621 agttttacat ttgattatat cattttttct tttctgtgta aattttttca aaagcttttt
     7681 caaggatttc ttcttcactg atttaatata aaaatttaca gataatttga aacctattac
     7741 ttggatttct caaaagaaat ctgctataat gaacgtgaat taaataatat taaggagctt
     7801 gttcacatga ttaaagaact aaaagaattt ttatttaagg gtaatgtttt agaccttgct
     7861 gttgctgtta ttttaggtgc agcttttaat gctatcatca cttcacttgt taaagacgtt
     7921 attacaccgc ttatccttaa tcctgtatta aaagctgctg gcgtttcaaa tatcgcacag
     7981 ctttcatgga atggtgttgc ttacggtaat ttcttaagtg cagttattaa tttcttaatc
     8041 gttggtacta ctttattctt catcgttaaa gctgctaaca aggttatggc taagaaacct
     8101 gctgaagaag aaattattga agttgttgaa ccaacacaag agcaattact tgctgaaatc
     8161 cgcgacttac tcgctaacaa ataataaaag actcggatta
//
LOCUS       DATRNX010000082         8050 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRNX010000082 DATRNX010000000
VERSION     DATRNX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            119..1045
                     /locus_tag="VBC81_001520"
     CDS             119..1045
                     /locus_tag="VBC81_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6912554.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            1216..1932
                     /locus_tag="VBC81_001521"
     CDS             1216..1932
                     /locus_tag="VBC81_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6912555.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            1934..2767
                     /locus_tag="VBC81_001522"
     CDS             1934..2767
                     /locus_tag="VBC81_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6912556.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(2819..3622)
                     /locus_tag="VBC81_001523"
     CDS             complement(2819..3622)
                     /locus_tag="VBC81_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6912557.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            3788..6238
                     /locus_tag="VBC81_001524"
     CDS             3788..6238
                     /locus_tag="VBC81_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6912558.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            6320..8035
                     /gene="pabB"
                     /locus_tag="VBC81_001525"
     CDS             6320..8035
                     /gene="pabB"
                     /locus_tag="VBC81_001525"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6912559.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         2643 a         1205 c         1583 g         2619 t
ORIGIN      
        1 tatcaaaaaa tactgaaaaa gtatttgaca aatactttat aataggttat gctaatatca
       61 aaattaagtt aacttaattt aatacataac ttatattaat tttaaaagga gcattttgat
      121 gaaaaagtgg ttagttattg tatcatgttt tgtagcattt ttaggcttag gtgcctgtgc
      181 taacaagcag gatcaagcca aaaacgaaaa attaaaagta gttgtaacca actctatttt
      241 ggcagatata acaaaaaata tagccggtaa caaaatcgac ttacatagta ttgtacctgt
      301 tggtaaagat cctcatgaat atgagccatt accagcagac gttgaaaaaa caactcaagc
      361 agaccttgtt ttttacaacg gaattaatct tgaaacaggc ggtaatgctt ggtttacaaa
      421 attaataaag aacgctaaga agaaagaaaa caaagattac tttgcggtta gcgatggagt
      481 tgatgttatt tatctaaatg gtcaaagcgg aaaaggtaaa gaagacccac atgcttggtt
      541 gaaccttgaa aatggtatta tttactcaaa aaatattgct aagcaattaa ttgctaaaga
      601 tccaaaaaac aaagctactt atgagaaaaa cagagatgct tatgtagcta aattagaaaa
      661 attggataaa gaagcaaaat ctaaatttaa tgccattcca gctaataaaa aattaattgt
      721 tactagtgaa ggttgcttta aatatttctc aaaagcttat ggagttccat cagcatacat
      781 ttgggaaatt aacactgaag aagaaggtac accagatcaa attactagct tagttaagaa
      841 attaaaacaa gtaagacctt cagctttatt cgttgaatca agtgttgaca agcgtcctat
      901 gaaatctgtt tcaagagaat caggtatccc aatttacgca gaaatcttca cagactcaat
      961 tgctaaaaaa ggtcaaaagg gtgacagcta ctacgctatg atgaaatgga atcttgataa
     1021 gattgcagaa ggtttagcaa aataagagtg agaatcgaca ttgaaatcat aatgaaatca
     1081 atgatgtagt attctcgctt aaggctgagt tgtaacaatg tcgaaatcct aatctaaaaa
     1141 ctgacttagc tttaataaat acagagaagt ggagtctggt tttataacca gctccattct
     1201 acttttggag gaaaaatgat tattagtaaa cacttaagcg taagttatga taataattta
     1261 gtgctagaag atattaattt acgcttagaa ggctccggaa tcattggtat tttagggcct
     1321 aatggggcgg ggaaatctac gcttatgaaa gctcttcttg gtttggttga ttctactgga
     1381 gaatcaggga ttgggggaga tttgctacct ttaatgggga gagtagcata tgttgaacaa
     1441 aaaactaata ttgattacca attccctata actgttggag aatgcgtttc tttaggatta
     1501 tataaggaac gaggcctctt caagcgacta tcaaaaactg actgggaaaa agtatcccga
     1561 gtgattgatc aagttggttt gagagggttt gaaaatagac caattaatgc cttatcaggt
     1621 ggacaatttc aacgtatgct tatggcaaga tgtctcgttc aagaagcgga ttacattttt
     1681 ttagatgagc cgtttgtagg gattgattcc attagtgaac aaatcatagt taatttacta
     1741 aaaaaattaa gtaaggccgg aaaattaatt ttagtcgtcc atcacgattt gagtaaagtt
     1801 gatcattatt ttgatcaagt aattattttg aaccgtcatc taattgcttg tggaccaata
     1861 gatcaggcat ttacccgtga gaatttaagt gctgcttacg gtgatgctat tttgttagga
     1921 caaggagact agaatgttta cgaaattttt tgaaggctta ttgacttatc attttttgca
     1981 aaatgctttt ataactgcaa ttgtgattgg tattgtagcg ggtgcagtag gctgttttat
     2041 tatcttgcgc tcaatgtctt tgatgggtga tgctatttct cacgctgttc taccaggtgt
     2101 tgctatatct tttattttag ggattaactt ttttataggt gctattgtct ttggtttgtt
     2161 gtcttcgatt atcattactt atattaaaga aaatagtgtc ataaaaggag atactgctat
     2221 tggaattact ttttcgtcct ttttagcctt aggtatcatt ttaattggac ttgctaatag
     2281 tacaactgac ctctttcata ttctttttgg gaatattttg gcagtacaag atagtgataa
     2341 atatatgact attattgtgg gattaattgt actgactctg attactatct tctttaaaga
     2401 attattattg acttcttttg atcctgtttt agcaaaatca atgggaatga gagtttcttt
     2461 ttatcattat ttattgatga ttttgttaac attagttgca gtaacagcta tgcagagtgt
     2521 gggaacaata ttgatcgtag cacttttaat tacaccagct gcaacagcat atctttatgt
     2581 taaaagtctc agaacaatgt tgtttttatc atctgcatta ggagcagtag catctgttct
     2641 ggggttatat atcggttata catttaatat tgccgcagga tcaagtattg ttttaacatc
     2701 tacttttatg tttttacttg ctttcctatt ttctcctaaa caaagtttgt ttaaaaagaa
     2761 acaatagtgt tgttaaaaaa tgagactaga aaatgttcta gtctcatttt tgatagaatt
     2821 atttaaattt gtagtcttta ataactttga tacttgtaat agtaattttt tctttcggtt
     2881 gatctgattt tgtaacttct accgaagcaa ttttatccac agtttccatg ccagagatta
     2941 cttgtccaaa gacggtataa ccaccatcta aacttggatt tcctccctct gaataggctt
     3001 taataatcac cttaggaact tttttatctg acagttgttt ggaatgatct tgctggcttt
     3061 gattgataaa gaattggctg ccattagtat cagctccagc atttgccatt gctaaacttc
     3121 ccctgatatt gtagaggtaa ggggatattt cattaacaaa accatttcca gaatctttct
     3181 ttttatcttt gctattccat atagattttc caccggtccc gtctccatta gggtcaccag
     3241 attgtatcat aaaatctttg ataactctgt ggaaacttaa cccattatag tatccttctt
     3301 ttgcatgtgt tagaaaattt tcaacagcta aaggagcata ttttggaaat aacttgatat
     3361 ttatatcacc tttagaggtt tttataagca cttgtgcctc gtttttggca acagccttgt
     3421 ccaattgagg aaaagctttt ttatcagctt ttaaggcttg cttagttttt ttgtttagtt
     3481 gttcagtagc ttcctttgaa gagttttcag ctagtttttg gtcaacataa cggtctcctt
     3541 ttagagaacg ctcaattgac tcgcaaccac ttaaagttaa tatactgaca caggctaaac
     3601 caagataaat aatttttttc atacatactc cttgattaag ttattaagat gattatattt
     3661 tatcataaat ttttaaataa tttataaaag taaatagtaa acctattggg ggacttgttg
     3721 aaattatgat agaatagtat agcatgagtt gatagagaaa gtgaggaaca agtatttcac
     3781 ttgaaatatg gtatttatgg caaataagaa aaaaacaaaa ggaaagaaaa ccagaagacc
     3841 tactaaggca gaaatagagc gtcaaagagc tattcaaagg atgattactg ctcttgtttt
     3901 aacaattatt ctcttctttg gtattatcag attaggtatt tttggtatta cagtctataa
     3961 cgtcatccgt tttatggtag gtagcttggc ttacttattt attgcggcaa ctttaatcta
     4021 cctttatttc tttaaatggt tgcgaaagaa agatagctta gtagcaggtt ttttgatagc
     4081 ttctttagga ttattgattg agtggcatgc ttaccttttc tcaatgccta ttttgaaaga
     4141 taaagaaatt ttgcgttcaa ctgctcgatt aattgtgtct gatttaatgc aatttaaaat
     4201 cactgttttt gccggtggag gtatgttggg tgctttgatt tacaagccaa ttgcttttct
     4261 cttttctaat attggtgcct atatgattgg tgttctcttc atcattttgg gtctcttttt
     4321 aatgagttct ctggaagttt atgacatcgt cgaatttatt agagctttta aaaataaagt
     4381 ggcagagaag cacgagcaaa ataaaaagga gcgttttgct aagcgagaga tgaaaaaagc
     4441 aatcgctgaa caagagcgca tagagcgtca aaaagctgaa gaagaagctt atttagcttc
     4501 ggttaatgta gaccctgaaa cgggtgagat tctagaggat caagctgagg acaatttgga
     4561 tgatgcgcta ccacctgagg taagtgaaac atcaactccg gtatttgagc cagagatcct
     4621 tgcttatgag acatcgcctc aaaatgatcc tttaccagta gagccgacaa tttatttaga
     4681 agactatgat tcgccgattc ctaatatgag agaaaatgat gaggaaatgg tttatgattt
     4741 agatgatgat gtagatgata gtgatataga aaatgtcgac tttacaccta aaacgacact
     4801 ggtttataaa ttaccaacga tagatttatt tgcaccagat aagcctaaaa atcaatccaa
     4861 agaaaaggat ttagtccgaa agaatatcag agttttagaa gaaacattta gaagttttgg
     4921 tatcgatgta aaagtagaac gtgctgaaat tggaccatca gttactaaat atgaaattaa
     4981 accagcagtt ggagttcgtg tgaatcgtat ttcaaatcta tctgacgacc tagctcttgc
     5041 tcttgcagca aaagatgtgc gtatagaagc accaattcct ggaaaatcat taataggtat
     5101 tgaagttcct aactcagaaa ttgcaacggt ttctttccgc gaactttggg aacaatctga
     5161 tgccaatcct gaaaaccttt tagaagtacc actaggaaaa gctgttaacg gcaatgctcg
     5221 cagttttaac ttagctagaa tgccgcatct tttggtagct ggttcaactg gttcaggtaa
     5281 atctgtggca gttaatggaa ttatttcaag tattttgatg aaggcacgtc cagatcaagt
     5341 taagtttatg atgattgatc ccaaaatggt tgaattatct gtttataatg atattccaca
     5401 tttattaatc cctgttgtaa ccaatccgcg taaagcaagt aaggcactcc aaaaagttgt
     5461 tgatgaaatg gaaaatcgat acgagttatt tagcaaaatt ggtgtgcgta atatagcagg
     5521 ttataataca aaggttgaag agtttaatgc ttcctctgag caaaaacaaa tacctttgcc
     5581 tttaatcgtt gtcattgtag atgaattggc tgacttgatg atggttgcta gtaaagaagt
     5641 tgaagatgct attattcgtt tggggcaaaa agcacgtgct gcaggtatcc atatgattct
     5701 tgcaactcaa cgtccatccg tagatgttat ttctggtttg attaaagcaa atgttccgtc
     5761 gcgtattgca tttgctgttt caagtggtac tgatagccgt acgatccttg atgaaaatgg
     5821 tgctgaaaag ctcttgggac ggggtgacat gctctttaag cctattgatg agaatcatcc
     5881 agtacgacta caaggttcct ttatttcaga tgatgatgtt gaaaggatcg ttggttttat
     5941 caaagaccaa gccgaggctg actatgatga tgcctttgat cctggagaag tatctgaaac
     6001 agataacggc tctggtggtg gcggcggagt acctgaaagt gatcctcttt ttgaagaagc
     6061 caagggactc gttttagaga cgcaaaaagc aagtgcctca atgattcaac gccgattgtc
     6121 tgttggtttc aatagagcaa caagactaat ggaagaatta gaagcagcgg gggttattgg
     6181 tccagcagaa ggaaccaagc cacgaaaagt tttaatgact ccaactccga gtgaataaga
     6241 aaaaactaat ctgacaagaa attgtcggat tagtttttta tgttataatg aagcaaatag
     6301 atgaagaaga aggttacgaa tgcatattga gactgttatt gatttcaaag aattaggaaa
     6361 aagatatcgt tttaaaaatc ctacaaaaga attaatagct gatactttag aacaagtctt
     6421 agaagtgata aaagaagttg attattatca atctcaaaat tattatgttg ttggttattt
     6481 atcttatgaa gcatctgctg cttttgattc acattttaaa gtttctcaac agaagttggc
     6541 tggagaacat ctagcttatt ttacagtaca taaagattgt gagaacgaag cttttccttt
     6601 aagttatgaa aatgttagat tagcagataa ttggactgct aatgtttctg agcaagaata
     6661 tcaagaggca attgctaata ttaaaggaca aattagacaa ggaaatactt atcaagtaaa
     6721 ttatacacta gagcttagcc aacaattatg ctcggatccc tttagtgttt atgaacgcct
     6781 aatggtagaa caaggagcag gatataatgc ttatattgcc tacgacgata aaaggatttt
     6841 atctgtaagc ccggaattgt tctttaagaa gaaggatgaa gtcttaacaa ctagaccaat
     6901 gaagggaaca agcgctagaa agcctactta tcaagaagat gtggcggagc gagattggtt
     6961 ggcaaatgat cctaaaaatc gttctgaaaa tatgatgatt gttgatttat taagaaacga
     7021 tatgggccgt atctgtgatg ttggaacagt taaggtaaaa aaactatgtc aagtggagca
     7081 gtatgcaact gtgtggcaaa tgacatcaac tattgaagga gttttatcgc cagaagtgac
     7141 acttatgtct atttttcaag ctctatatcc ttgtggatct atcactggag ctcctaagat
     7201 atcaacaatg gctattatta atgagttgga aaaacggcca agaggtattt attgtgggac
     7261 gataggactt tgcatgccag acggacaagc tatttttaac gttcctattc gcacagtaca
     7321 aatgaaaggt caacaagcct attacggcgt tggtggaggt attacgtggg agagccagac
     7381 agattctgaa tatgaagaaa cccgtcaaaa atcagctgtt ttaacacgtg tcaatccaaa
     7441 atttcaatta ataactacag gaagagtgac tgaaaataaa cttcttttct ctcaacaaca
     7501 tgtcgagaga ttagttgaat cagcgtctta ttttgcttat tcttttgata aaagtaaatt
     7561 cgaaagggaa ctgaaaaaat accttcatca gctagatgag aaggattacc gcttgaaaat
     7621 aatgcttgat aaaactggta aggtaacgtt tgaggtaaaa caactagtga atttatcaaa
     7681 aaagttttta acggcagagg tggtcgtaca agactaccct attaaattat ccccgtttac
     7741 ttattttaaa acttcttatc gcccacatat tattgaaggt cagaatgaaa agatatttgt
     7801 atctcctgag gggttgctat tggaaacaag tattgggaat attgttttag aaaaaaatgg
     7861 aaggttttta accccagatt tatcagaagg agggttgaat gggatttatc gtcgtcatct
     7921 ccttaaaaac caaaaagtaa ttgaagcacc actaacttta aaagatttag aatcagccga
     7981 tgctatatac gcctgtaatg ctgttagagg gctttatcct ctaaacctaa agtaaaaaga
     8041 gttggaaatt
//
LOCUS       DATRNX010000083         8020 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRNX010000083 DATRNX010000000
VERSION     DATRNX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..454)
                     /locus_tag="VBC81_001526"
     CDS             complement(<1..454)
                     /locus_tag="VBC81_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912560.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDK"
     gene            636..1622
                     /locus_tag="VBC81_001527"
     CDS             636..1622
                     /locus_tag="VBC81_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912561.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            1615..2526
                     /gene="rbsK"
                     /locus_tag="VBC81_001528"
     CDS             1615..2526
                     /gene="rbsK"
                     /locus_tag="VBC81_001528"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6912562.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            2501..2899
                     /gene="rbsD"
                     /locus_tag="VBC81_001529"
     CDS             2501..2899
                     /gene="rbsD"
                     /locus_tag="VBC81_001529"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6912563.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            2915..4393
                     /locus_tag="VBC81_001530"
     CDS             2915..4393
                     /locus_tag="VBC81_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6912564.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            4395..5336
                     /gene="rbsC"
                     /locus_tag="VBC81_001531"
     CDS             4395..5336
                     /gene="rbsC"
                     /locus_tag="VBC81_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6912565.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLTPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            5389..6357
                     /locus_tag="VBC81_001532"
     CDS             5389..6357
                     /locus_tag="VBC81_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6912566.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(6397..7851)
                     /locus_tag="VBC81_001533"
     CDS             complement(6397..7851)
                     /locus_tag="VBC81_001533"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6912567.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         2588 a         1481 c         1463 g         2488 t
ORIGIN      
        1 ctttatcacc taactttatc ccttcagctt ttaaactatc atttaaaata atagcttttt
       61 tgtaacttgt cagttgtttt ccctcagaaa gtgtaggtgc cataaaagaa tctttatcaa
      121 tagcaaaata agaaatatca attttctttt catgtccttg tcgagttaaa ctcgaacgtt
      181 gaatatttaa agtggtacta tcttttaaat tcaaactgtt taaatctgtc tgatctttag
      241 ttgtcaatac agatgatgta atcacttgtt cagcaccttc attcaaaata taggtttgag
      301 ctgggttatt ttcaatagca gcactaacag ctctacctaa accgtttgct aaacctgata
      361 aaaaaactac catgaacatc ataacaatga ttagcaattc aatcaggagg tattttttct
      421 tataaaaggt taattctttc caagcatgtg acatggtttt tctcctttga tcttttgatg
      481 tgaacatagt atcacagctt tatgacttga atatgacctc taaaaataat gatattttat
      541 cattaaatta tttaaaaaat agcttgacaa ttttttagta agcgattaca attctaatta
      601 tgaaaccgtt cccacaataa aagaaaggag tttctatgag tacgattaga caagtcgccg
      661 agaaagctgg cgtatcaaca tctactgtct caaggtatat atcccaaaat ggttacgtta
      721 gtcaaaaagc aagtcaaaaa atcgagcaag ctatccgcga actccattat gttccaaatt
      781 tccttgcaca atctctgaaa accaaaaaaa atcaacttgt tggattacta cttcccgata
      841 tttcaaaccc tttctttcca agacttgcta gaggagttga agaatttcta aaagaacagg
      901 gttatcgtgt catgcttggc aacacaaata acaagagcca tttagaagaa gagtacttaa
      961 atgtgctatt acaaagtaat gctgcaggga ttattacaac acacgatttc accaaaaatc
     1021 atcctgaaat agatattcca gttgtagttg ttgaccgcgt taatcaagaa acacagtacg
     1081 gcgttttctc tgataataaa gagggaggaa aattagcagc tcaagctatt tggacagcag
     1141 gtgctaccaa tatattactc attcgtggtc cgctagataa agctgataac cttaatcaaa
     1201 ggtttcaagg aagccaaaat tacctattaa acaaaggggc tcgttttgct atcgaagata
     1261 gtgccagttt tgattttgca gagattcaaa ttgaagcgaa aactttactt gaccaccatc
     1321 cagatattga tagcattatc gctccatctg atatacatgc tattgcctac ctacacgaga
     1381 ttcttaatcg agggaagcga atccctgaag atgtacaaat tattggttat gatgatattt
     1441 taatgagcca atttatctac ccttcgttgt caactattca ccaatcatca tatattatgg
     1501 gacaaaaagc agctgaatta attttcaaaa ttactaatca attacctatt accaataagc
     1561 gtattaaatt acccgttcat tatgtcgaac gtgaaaccct aaggaggaaa atagatgagt
     1621 aacattgtca ttatcggtag tatttccatg gacttagtta tggaaaccaa tcgtatcgcc
     1681 aaagagggag aaaccgtatt tggccaacgt ttttcaatgg ttccaggagg taaaggtgct
     1741 aatcaggctg tcgctattgg gcgtcttagc caagatcgag ataatattac tattcttggt
     1801 gccattggcg aggattcttt tggtcccatt cttttggata atttaaacaa aaaccatgtt
     1861 actacggatt ttgtgggaac gataccatct tcttcaggtg ttgcccaaat cactctttat
     1921 aataatgata atcgaatcat ttactgtcct ggagccaacg gaaaagttga tactaaaaag
     1981 tggtctcagg agtggtcaat aattaaagaa gctgatttag ttgtattaca aaatgaaatt
     2041 cctcatcaag caaatatgaa aattgctaat ttttgtaaag aacatagtat taaattactc
     2101 tataatcctg caccatctag aaagactgat attgagatgc tcgataaggt cgattatttc
     2161 acaccaaatg aacacgaatg tcaagagctg ttcccaaacc aaaaactaga ggatatatta
     2221 gccacatatc ctgaaaaact aattgtaaca ctcggcacaa aaggagccat ctactctgat
     2281 ggaaaagaaa gccatttaat tcctgcctta gaaactaaag ctgttgatac aactggtgca
     2341 ggtgatactt ttaacggtgc attcggttat gctatctcga aaaaatttaa gattgctaat
     2401 gccttacgtt ttgcaactct agctgctcac ttatccgtgc aaaaatttgg tgcacagggt
     2461 ggcatgccta caattaaaga aatggaggac aaccaacatt atgaaaaaga ctggtatatt
     2521 aaatagtcat ctcgctaaat tagcagatga ccttgggcat actgatcaag tttgtattgg
     2581 agatctgggt ctacctgttc caaatggcat tccaaagatt gatttgtctt tgacctctgg
     2641 aatacctagt ttccaagagg tacttgatat ctatttagag aatatcttag ttgaaaaagt
     2701 cattttagca gaagaaatca aggaagcaaa ttcggatcaa ttatcaaggt tattagctaa
     2761 acttgataac tctgttaaca tagagtacgt cagtcacaat catttaaaac aaatgaccca
     2821 agatgtcaaa gcagttattc gtacaggtga aaatacaccg tattcaaata ttattctaca
     2881 atctggtgtt atcatctaga aaggagttta gtccatgaaa attgacatga ggaatatctc
     2941 caaatccttc ggtactaata aagttctaga gaagattgat ttggaattac aatctggtca
     3001 aatacacgca cttatgggtg aaaatggtgc gggtaaatca accttaatga acattctaac
     3061 aggacttttt ccagcaagta caggaactat ttatattgat ggcgaggaac gaactttttc
     3121 aaaccctcaa gaagcagagg aatttggtat tagttttatt caccaagaga tgaatacttg
     3181 gccggaaatg actgtcttgg aaaatctctt tcttggacgt gaaatcaaga caacttttgg
     3241 cttactcaat caaaaattaa tgagacaaaa agcacttgaa gcttttaaaa gattaggtgt
     3301 gactattccc ctagacatcc cgattggtaa cttgtctgtt ggacaacaac aaatgattga
     3361 aatcgccaaa agtctcctaa accaactctc tattttggta atggatgaac cgactgctgc
     3421 tttaacagat cgtgaaactg aaaatctttt ccgagttatc cgaggtttaa aacaagaagg
     3481 agtgggcgtt gtctatatct cccatcgaat ggaggaaatt tttaaaatca ctgacttcgt
     3541 aaccgtcatg agagatggtg tcattgtaga tacaaaagaa acatcactca caaattctga
     3601 cgaattggtt aaaaaaatgg ttggccgaaa attggaagat tactatcctg aaaaacattc
     3661 tgaaattggg cctgttgctt ttgaagttag taacctctgt ggagataatt tcgaggacgt
     3721 ttccttttat gtccgtaaag gcgagattct tggattttct ggattaatgg gagctgggcg
     3781 tactgaagtt atgcgaacta tctttggaat tgataaaaag aagtctggta aggtgaaaat
     3841 tgataaccaa gaaatcacta ttactagccc aagccaagct atcaagcagg gcattggttt
     3901 tttgaccgaa aatagaaaag atgaaggtct catcctagac ttcaatatca aggacaatat
     3961 gactctccct agcactaaag attttagcaa acatggtttc tttgatgaaa aaacaagcac
     4021 aacatttgtg caacaattga ttaatcgcct ctacattaaa tcagggagac cagatttaga
     4081 ggttggaaat ctttctggag gtaaccaaca aaaggtcgtt ctcgctaaat ggattggtat
     4141 tgctcctaaa gtcttaattc ttgatgagcc aacacgtggt gttgatgtcg gagctaaacg
     4201 agagatttac caattaatga atgaactagc agacagaggt gttccaattg tcatggtatc
     4261 atcagactta cctgaaattc taggtgttag tgaccgcatc atggtaatgc acgaaggtcg
     4321 aatcagtgga gaactcagtc gaaaagaagc tgaccaagaa aaagttatgc agctagccac
     4381 aggaggtaag taaagtgaaa aatatgatga aatatatatc agagttaaca accctgattg
     4441 cattagctgg tttaatggtt attatcacac tcattaatcc taatttcctc acaaccaata
     4501 atctactcaa tttattatta caagtaactg caaatggttt tatcgctttc gggatgacct
     4561 tcgttatttt gactggcggt attgatttat cagtcgggtc catattagcc ctatctagtg
     4621 cattaacagc tggattaatc ggccacggag tacccgttgg tattgctatc gttttatctg
     4681 ttgccttagg tggcatcttg ggaatgctaa acggactctt catttcttac ggaaaattaa
     4741 caccatttat cgttacactg gcaacaatga ctatctttag aggagcaacg ctagtttatt
     4801 caaacggcaa cccaattacc gctggattat ccgatagttt tctattccaa tttttgggac
     4861 aaggctatat tgttggtatc ccattcccag tcattctcat gtttcttact tttattattt
     4921 tgtatatttt gttacataaa acagccttcg ggaaatctgt ttacgcttta ggaggtaatg
     4981 aaaaagcagc gtatatctcg ggtattaagt taaacaaagt taaaattatc atctacacta
     5041 tctcaggtat catggcttcc atttctgggt taattatcac atcacgcctt agctcagcac
     5101 aaccaacagc tggagcaagt tatgagatgg atgctattgc cgccgttgtc ttaggtggaa
     5161 cgtctttatc tggtgggaaa ggacgtatca ttggaacatt gattggtgct ctcattattg
     5221 gtgtcctgaa caatggatta aacatcattg gtgtctctgc attttggcaa caagttgtga
     5281 aaggtatcgt tattttaatg gctgttttac ttgatcgctt taaagtagca aaatagtctt
     5341 tactctaatt tctataagtt cattatattt tatctaaagg agtttcaaat gaaatttgga
     5401 aaaaaacttg gctttctagc cttattgatg tctattgtcc tcattctcgg tgcatgtggt
     5461 aaaactggac ttggaaattc ttctgggaat tctacaaaaa atgtaaccaa aaaatctgct
     5521 aaagatttga aactaggtgt atctatttca acaactaaca acccctactt tgttgcgatg
     5581 aaagacggta ttgacaaata tgcgagcaat aaaaaaataa gcattaaggt agctgatgct
     5641 caagatgacg ccgcacgtca agcagatgat gttcaaaact ttattagtca aaacgtagat
     5701 gctattttga ttaatcccgt tgactcaaaa gctattgtaa cagctattaa gtctgcaaac
     5761 aacgctaata ttcctgttat tctaatggac cgtggtagtg agggaggcaa agtattaact
     5821 actgtcgctt cagataacgt tgctgctggt aagatggctg cggattatgc cgttaaaaaa
     5881 ctaggcaaaa aagcaaaagc ctttgaatta tctggagtac caggtgcctc tgctactgta
     5941 gaccgtggta aaggatttca ttcagttgca aaatctaaac ttgacattct ttcaagccaa
     6001 tctgccaatt ttgaccgcgc caaagctttg aatactacac aaaatatgat tcaaggacat
     6061 aaagatgtcc aaattatttt cgcacaaaat gatgaaatgg cacttggggc tgcacaggct
     6121 gtcaaatctg ctggcttgca aaatgttctt atcgtaggaa ttgatggtca gccagatgcc
     6181 catgatgcca ttaaaaaagg tgatatttca gccactattg cacaacaacc agctaagatg
     6241 ggagaaatag ctattcaagc agcaattgat cattataaag gcaaaaaagt agagaaagaa
     6301 acgatttctc caatttacct tgttaccaaa gacaatgttg aaaaatacaa ttggtaaaag
     6361 tacacaaaaa gtcgtgaaaa ttcacgactt tttatattat aattttgcta acatattatc
     6421 gatatgttga actgattttt ctttacctaa taaataaata gtatctggta actctggtcc
     6481 atgcatttca ccagaaaccg caatacgaat aggcataaat aggttcttac ctttgatgcc
     6541 tgtttctttt tgaacagcct taatttgagg gaaaatagta tctctagtga attcttcgtc
     6601 agaaagcgat actaactttt ccttaaaagc agataagact gttgggactg tttctgctgc
     6661 cattacttct ttttcggctt ctgttaattc tggaaaatct gcaaagaaaa ggtctgttaa
     6721 tggtacaatc tcatcagccg attttaattg aggtttataa agctcaacta atttttcagc
     6781 tttgtctgta agtcgaccag cttcttctaa gaatggtttg caaagtgcaa aaacactctc
     6841 aaaatcagca ttttttaggt aatcattact catccaatcc atcttcttct gatcgaatgc
     6901 tgctggtgat ttgctaagac gattttcatc aaatagatta attaattgct cgcgtgagaa
     6961 gatttcttct tctccaccag gattccaacc aagaagggca atgaaattaa agacagcttc
     7021 cgacatataa ccttttttac gatagtcttc gataaattga agagtatttg tgtcgcgttt
     7081 tgataatttc ttaccagtct ctgagtttat aatcaatgtc atgtgaccaa attgtggcgc
     7141 ttcccaaccg agtgcttcat aaaccattaa ttgttttggc gtatttgcaa tatggtcatc
     7201 gccacgaata acatgcgaaa tttgcatgtc atgatcatca atgactactg cgaaattgta
     7261 agttggataa ccatctttct tctgaataac ccagtctccg ccgatattag agccttcaaa
     7321 ctcaatgtca ccttttacca tatcagtcca cttataaatc ccagattcat taacagcaag
     7381 gcgtactgtc ggaatgatgc ctgctgcttc acgttctgca atgtaagctt ctttttcagt
     7441 ttctgacatt ccgataaatt cgttgatata gcgaggtgtt tcgcctgcca attcttggcg
     7501 ttcacgctct gctgctaatt cttcctcagt cacataggac ttgtaggctt taccttctgc
     7561 taacaactga tcaatataac gttggtagag ctcaaggcgt tctgattgac gataattttc
     7621 atgtgtttca gggctttcat cccagtccat acctagccaa cgtaagttct ccaattgaga
     7681 gcgttcacca tcttcgacgt gacgttttcg gtcagtatct tcaatacgaa taacaaaatc
     7741 cccaccatga tggcgagcat aaaggtagtt aaagagcgca gtacgcgcat ttccgatgtg
     7801 taacaatcct gttggacttg gtgcataacg cacacgaatc ttattagcca aaattctact
     7861 cctatatttt ctgatagtaa actttttcag tttaactgaa ttagtcaagg gggcaaaaca
     7921 tcattcaaac tcttacagat gattgccaat caaccctatt ataccactaa atcactaaaa
     7981 atgataatca gtaataatag tcacactaat tttcgataaa
//
LOCUS       DATRNX010000084         7879 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRNX010000084 DATRNX010000000
VERSION     DATRNX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            92..751
                     /locus_tag="VBC81_001534"
     CDS             92..751
                     /locus_tag="VBC81_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6912568.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            802..1479
                     /locus_tag="VBC81_001535"
     CDS             802..1479
                     /locus_tag="VBC81_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6912569.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1472..2917
                     /locus_tag="VBC81_001536"
     CDS             1472..2917
                     /locus_tag="VBC81_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6912570.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2924..5083
                     /locus_tag="VBC81_001537"
     CDS             2924..5083
                     /locus_tag="VBC81_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912571.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5223..6635
                     /locus_tag="VBC81_001538"
                     /pseudo
     CDS             5223..6635
                     /locus_tag="VBC81_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            6875..7375
                     /locus_tag="VBC81_001539"
     CDS             6875..7375
                     /locus_tag="VBC81_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912572.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            7573..7821
                     /locus_tag="VBC81_001540"
     CDS             7573..7821
                     /locus_tag="VBC81_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912573.1"
                     /translation="MEKITNDFEKVIAVLNRLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
BASE COUNT         2748 a         1086 c         1515 g         2530 t
ORIGIN      
        1 taatttattg ttaatattta tatataaatt aatatttaat taaacagagg tgagtgatga
       61 acgaagttct tagacaggaa aaaaatattt aatatcactt gaagattact atcttttatc
      121 taagaaattt tcccaagtaa tgacgctcga ttcgcatagt tcagacgacg gctatactat
      181 ccgttcgcta tactttgatt ctttagaaga tgttgattgg caagaaaagg aagatggtgt
      241 tgagatacga agaaagattc gtttgcgcaa ttatggtagt caaagcaatt ttgcgaaatt
      301 agaaatgaaa caaaaacaag gtgctaatca aaaaaagcgc tctctaaaat taagcaagac
      361 agatgctcaa gcgctgatta atggtaacaa tagtgtttta ttaaattatc cggatacatt
      421 tgctgcggag tgttattcaa ggataacaac gcaactttat caacctaaaa cggttattga
      481 gtaccaaaga aaagcttttg ttgctaagga aaacaatatc cgaatcacct ttgaccacca
      541 tataataggt acagaggcta gttatgatat tttctcagag gatttaaatc aaaattattt
      601 attagatcct tatcttgtta ttttagaagt taaatataat ggatttttat tgtcttatat
      661 taaagaactc ttagattcgg tagatagaag tgaactttca gcgagtaagt attgtttagg
      721 aagagctgtt agtaaacatt atcgttttta gtgaaaaagt atatacgtta attattatta
      781 aatgtgagag gataacaaaa aatgaaagac ctattacgaa atagtctaga gcaaagtgga
      841 aatttaagtt ttcaagatat gattttacat attcttgtag cagctttatt gagtgtagtt
      901 atttatgttt cctatgctta tacgcatagt ggaactgcct atagtaaaaa gtttaatgtt
      961 tcattaatga cattgacggt cttgactgca acagtaatga ccgttattgg taataatgta
     1021 gccttgtcat tgggtatggt cggtgccttg tcagttgttc gttttaggac agccataaaa
     1081 gattcaagag atacagttta tattttttgg accatagttg ttggtatctg ttgtggtgtc
     1141 ggtgactatg tggtagctgc attaggaagt agcgttatct ttatcttatt atgggttatg
     1201 ggacgtgtta aaaacgagaa tcgtatgtta ttgattgtga agtgcgatag aacactagaa
     1261 gttgatttag aaggaatttt cttccaatat tttgacggaa aagctgttca gcgtgttaaa
     1321 aattcaacaa ctaatactat tgaaatgatt ttcgaaatct ctagaaaaga ttacgataag
     1381 caactccatg tagataatca gttaactgaa aaagtgtacc aattgggaaa tattgattat
     1441 ttcaacattg ttagccaaag cgacgaaatc aatgggtaga cttatgagag ggaaggttat
     1501 ttacggcaca acccttatag gtctttttct attcttattt ttctattttt ggattcctaa
     1561 gcatcacatc gagagaatac atcatcatcg tataaagcag gtagatgcga agagtgattt
     1621 aacaggattt aaaacccatt tgcccattat cagcattgat acaaagcaac aagttattcc
     1681 tcttgttaca aaagaaggcg gaaaatatgt caaagctagg gataatatta atgttgatat
     1741 cgaattacgg gattctccaa gtagatcaca tcatttatca gaaaagccga gaattaggac
     1801 aaaagggtta atatcatata gaggaaattc ctctcgttac tttgataaga agtcattgaa
     1861 agttaagttt gttactaata agttaaagga aaagaagcat cgattagcag gaatgcctaa
     1921 agaatcggag tgggtattgc atggtccctt tctagacaga acattattaa gaaattatct
     1981 gagttataat attgctggtg agattatgtc ctatgcccca aacgttcgct actgtgagtt
     2041 atttgtcaat ggtgagtatc agggagtata tctagcagtt gagaatatcg aacaaggtga
     2101 gcaacgagtt cccattgaaa aaagtgataa aaagttgcat aaaacacctt atatcgttgc
     2161 ttgggatcgt gaacataagg caaagcaaaa gttagataac tatgtacatt atacacacca
     2221 gtctggtatc tcagctcttg atgtgaaata tccaggtaaa caaagattga catcaaaaca
     2281 attagagttt attaataagg acatcaatca tattgaaaaa gtcctttatt cttatgattt
     2341 ttcacaatat cctaaatata ttgatagaga atcatttgca aattattttg tcatcaatga
     2401 attttttaga aatgtggatg cgggtaaatt ttcaacctat ctatataaag atcttcgtga
     2461 tagagcaaaa ctagtagttt gggattttaa taatgctttt gataatcaaa ttgaaggacg
     2521 tgttgatgag gcagatttta ccttgacgga tgcaccgtgg ttcaatatgc tcattaagga
     2581 caaagcattt atagacctgg ttgttcatcg ctataaagaa ttacgaaagg gagttctagc
     2641 gacagaatat ttatctaatt acatagacga gacaaggcat tttttagatc cagctattga
     2701 tcgaaattat aaaaaatggg gctatgtctt tgatttaaag aatactgatc caaggaatta
     2761 tttgattcct actgaacgca atgtgacaag ttatcacaaa tcagtagaac aattaaaaga
     2821 ttttatcaaa aagcgtggca gatggatgga tagaaatatt gagaccttgt atcaatatag
     2881 tgcaccatcg aaaaatacta atactttact ggagtagaac attatgaaaa aatatctaag
     2941 tattgtagcc cttttaacta ttttgtttag ctctatttat tatttgtatt actttgatgg
     3001 tagtttgtat ttaccaaagg gcttattaaa agaaaataca agaactaact ttgttgttaa
     3061 aggtgatact gtacttcaca agcccaccaa taaacctttt gttgttaaag gagtagacgt
     3121 tgagtcttcc ttagcgggtt atcatcacaa cgattttcct attactcaaa aaacgtatcg
     3181 tgaatggttc catttaattt ccaacatggg ggcaaatact gtaagagtca aggtaccgat
     3241 gaatgttgca ttttacgatg ccttatatca ccacaacaaa gaatcaaaga ggccactgta
     3301 tttgttgcaa ggaatacgta tagattctta tcgcaataat gcttctataa cagcttttaa
     3361 tgataattat agggggtatt taaaacgaga agcaaaaggc gttgtggata ttctccatgg
     3421 gcgtaagcaa gtatggaata ctgattttgg tagccgtcat tatcattatg atcttagtcc
     3481 ttgggtactt ggttatgtcg taggggatga ttggaatagt ggtactgtcg cttatactaa
     3541 tcatcaagag aaaaaaacgc aatataaagg acgttatttt aaaacttctg tggcagctaa
     3601 tccatttgag gtcatgctag ctcaagtaat ggatgaattg acacattatg agacagctaa
     3661 atatggttgg caacatttga ttagtttttc aaactcacca acaacagacc cttttcatta
     3721 tcgaaaacca tttgaggcac aggctcctaa atacgtacaa ctaaatgtag aaaatattca
     3781 agctaattca aatgttaaag caggtatgtt tgcagcatat aaagctattg atttccatcc
     3841 tcgatacaag gattatctat tatttgataa agagaatatc agtaaagaag atagacaaaa
     3901 gattaaagaa ctttctttgt cacagggata cgttaaactg ctaaatgctt atcacaaaat
     3961 ccctgttcta gtcacgggtt atggctattc gacagcgaga ggtattgccc aaaaagaaat
     4021 tgataaacgt cctctgccga ttaatgaaaa agaacaaggt cagcgtttac tagaagatta
     4081 tgaatctttt atatcatccg gtagttttgg agcgactatc aatgcatggc aagacgattg
     4141 gaatgcaagg gcgtggaata catctttcgc cacaaataaa catagtcaat tcctatgggg
     4201 ggatgcacaa gtatttaatc aaggttatgg tttattaggc tttaaaaacg caaaacatca
     4261 ttatcaagtt gatggtaaaa gaggcaaagg agagtggaaa catcctctga tgactagtgc
     4321 aacaggagat gacttatatg ctagcagtga tgaaagctat ctctaccttg cgattaaaac
     4381 aaaacctgaa aaactaaaag aaaaacgatt attaccaata gatattacac caaaatctgg
     4441 tagtagaaaa atgaatggta gtaaggtcac attttctaaa tctagtgact ttgtattgtc
     4501 tattgatcca aatggcaagt ctgaattatt tgtccaagag cgctataatg ccttaaaagc
     4561 gaactatctt cgacagctta acggtaaaga tttttatgct ttcccaccaa agaagaacag
     4621 tagtaatttt gagcagataa atatggtatt gagaaataca aagattgttg aagacatgga
     4681 aaaagtaaaa gcaacagaga ggttcttacc aactcatcct actggtcttc tcaaaacagg
     4741 aacaactgat aggcaccaaa aaacatttga ttcacaaaca gatatttcgt ttggaaagga
     4801 ctttatagag gtcagaattc cgtggcagtt gttgaatttt tctgatccat catctcaaaa
     4861 aattcacgat gattacttta aacattatgg tgtgaaggag ttagaaattg agagcattgc
     4921 tttaggatta ggtgctaata gcaaagaaaa cacactgata aagatggcag attatcgttt
     4981 gaaaaattgg gagagacccg ataccaaaac ctttttaaaa gactcctatt atagtattaa
     5041 gaaagaatgg tctaaagaaa gagagagaac atatggtaca taatatttta tatttttaca
     5101 ttttgatttg cttagcaatt attgttttta acattcacta tatcttaaga caacgattag
     5161 ataagcttag ttggcttcgt attcggaaaa aggatataaa tatcaaataa aggggatttt
     5221 ctatggcatt attttttacc ttcctaaagg tatctcaagt cattttctta gtattattga
     5281 ttgcctatgc cagcttttta gtgatttcat tagtcattgg aggtgttcat ctctatgaaa
     5341 aaaatgagcg caaaaaaagt ttaactagaa ataaagaaaa aattccaatt tcaattattg
     5401 taccagccta taatgaatca acaaccattg tgagttcgat tgattcgtta ttgcaccttg
     5461 attatgaagc gtatgaaatt atcgttgtgg atgacggttc cagtgacaat acttcagatg
     5521 ttctcaagga agagtttgca ttaatgaaaa tctcgaatac gattgattca attattgcta
     5581 ctcaaacttg taaagatgtc tttcagagac aggttggtaa ggttaaactg acactcattg
     5641 ttaaagaaaa tggtggcaaa ggggatgcct taaatatggg gatcaatgct gctaattacg
     5701 attattttct atgtctagat gcagattcta tgttgcaggt agattctttg agtcaaatta
     5761 gcaaatccat acaggtagat ccgacggtta tagcagtggg agggcttgtc caagttgccc
     5821 aaggtgttaa gattgagcag gggaaagtag catcttatcg tttgccttgg agaattattc
     5881 cttgtgcaca agcacttgag tatgattcat catttttagg agctcgaatc tttttagact
     5941 atttaagagc taatttaatt atttcggggg ctttcggtct ttttaagaaa gaccttgtta
     6001 aagcggtggg aggatatgac acccagactc taggagaaga tatggagttg gtgatgaaat
     6061 tacatttctt ctgtcgtaat aataatattc cttatcgtat ttgttatgaa acagatgctg
     6121 tttgttggag tcaagcacca acgaatttag gggatttacg taaacagaga cgccgatggt
     6181 atcttggctt gtactaatgt ttaaaaaaat ataaaagtat ttttgccaat tatcgctttg
     6241 gagctgtagg atcaatttct tatatttact atattttatt tgaattactg acccctttta
     6301 tagaatgttt tggtattgtc attatatttt tgtccttatt atttaatcaa ttaaacatcc
     6361 cattctttat atcattggtg agcctttata tcttttactg tgttttaatt acattaagct
     6421 ctttcttaca ccgtatttat tctcagcaac tagtaatagg aattttagat attgttaaag
     6481 tattttatat tgctgttttt aggtatctta tcctacatcc tgtattaact tttgttaagg
     6541 ttgcaagtgt tattggctat aaaaataaaa aaatggtttg ggggcatatt acgagagaga
     6601 aacaaatgaa ggattttgaa acaagtgtta cttagaagtt atagcgattc atatatataa
     6661 aataatcact aaaagagcgt aattatctac gctcttttag tgattatcaa taattggcct
     6721 aatctgaggt taatgtttaa aaccatctaa aattttagaa atataatgat aaatgagaac
     6781 cagaactgtt caaaataaaa attaatatca cgaaaatggt ataattggag tagttatcag
     6841 gcagttaata ataatttttg aaaggatttt agttatggct tgcataatca ggagagcaag
     6901 attaggagat gaagtcaatt tagcttatat tcaaacggag agctggaaag ctgctttggg
     6961 taagatacta cctgaggata tcattcaaaa aactacggaa atagaacctg ccataacaat
     7021 gtatcaacag ctactacaca aggaggttgg taaggggtat atattggaag tagatagcaa
     7081 tcctcattgt atggcttggt gggataaaag tagagaagat ggtatgcttg attatgcaga
     7141 actcatttgt atacatagtc ttaaagaagg ttggggaaaa ggttatgggt ctcaaatgat
     7201 gaatcatgta ctatcagaga tacaacaagc aggttataat aaagtgatac tttgggtatt
     7261 tacagaaaat acacgcgcaa ggaaatttta cgataggttt ggtttttctt ttaagggtaa
     7321 atcaaaaact tattttgaaa aggaagaaat gtgttatgag aagattttgc actagcgaga
     7381 taaagcatcc gataatacaa tagtataaac atcctatttt gaaaaacaat ttcctttaag
     7441 gggaacaaat ttgtaaaaga aaattgttat aggtataatg aagattatga aggtaaaatt
     7501 ttttgagtga aaaacagtgt atcaatttta ggttctctag tctacaaata gttatgctaa
     7561 ctctatggaa gaatggagaa aattacgaat gattttgaaa aagttatagc tgtgctaaat
     7621 cgactaagta tttcttatga attagtggaa aatggacctg ttttgttgac ggagcaagca
     7681 gataacttta ttgtgggaat tgaaggagta agaacaaaaa taatgttctt gaccaacaag
     7741 aaaaagacga aatattattt actaataatg gatgaccaga aactatttta tcctatgaaa
     7801 acaatagtag cctttagatg aactaaaagg cgaagaaact ttaaattatg attttaaata
     7861 agcaataaac cttgatata
//
LOCUS       DATRNX010000085         7655 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRNX010000085 DATRNX010000000
VERSION     DATRNX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            26..325
                     /locus_tag="VBC81_001541"
     CDS             26..325
                     /locus_tag="VBC81_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6912574.1"
                     /translation="MLELKFQQERMLKGLHIKNTDQLIFYDYRFGMMSNDAVNKFLAS
                     SLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLL"
     gene            complement(461..531)
                     /locus_tag="VBC81_001542"
     tRNA            complement(461..531)
                     /locus_tag="VBC81_001542"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Thr,seq:ggt)
     gene            complement(602..1436)
                     /locus_tag="VBC81_001543"
                     /pseudo
     CDS             complement(602..1436)
                     /locus_tag="VBC81_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            1747..2001
                     /locus_tag="VBC81_001544"
     CDS             1747..2001
                     /locus_tag="VBC81_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6912575.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2110..2421
                     /locus_tag="VBC81_001545"
     CDS             2110..2421
                     /locus_tag="VBC81_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912576.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2689..3258
                     /locus_tag="VBC81_001546"
     CDS             2689..3258
                     /locus_tag="VBC81_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912577.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3356..3940
                     /locus_tag="VBC81_001547"
     CDS             3356..3940
                     /locus_tag="VBC81_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912578.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3937..4503
                     /locus_tag="VBC81_001548"
     CDS             3937..4503
                     /locus_tag="VBC81_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6912579.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4503..7154
                     /locus_tag="VBC81_001549"
     CDS             4503..7154
                     /locus_tag="VBC81_001549"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6912580.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(7372..>7655)
                     /locus_tag="VBC81_001550"
     CDS             complement(7372..>7655)
                     /locus_tag="VBC81_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912581.1"
                     /translation="QYTSRNKVGSSYTAHSVIEEIYLYLIAKESILYFESLDENSHLQ
                     LKELLDYNDEWPFDIFDDMDSYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
BASE COUNT         2465 a         1323 c         1563 g         2304 t
ORIGIN      
        1 agtaagtcat gatttaaaag agttattgct tgaactaaaa tttcaacaag aaagaatgct
       61 gaaaggtttg catataaaaa atacagatca attgattttt tatgactata ggtttggtat
      121 gatgtcaaat gatgctgtta acaaattctt ggcaagctca ttggaaaaat tagaaataga
      181 atctaagatg tcatcaacag gtgctagaca tacttatgga agttatttgt tggctaacgg
      241 agtagatatt tgggcggttg caaaattaat ggggcataaa gatatcaaac aattaataga
      301 aacatatggt catttgcttc tgtaagtgga agaaaaggaa aatgatcagc ttttaaaaat
      361 tcttaataat aaaggtagtt tttaaaatga ctatcaagaa caaattaaga acaaaaataa
      421 aagaaaccct attataataa ggtttcactc atatattttc tgccaccacc gagaattgaa
      481 ctcggaattg agcattacca ttgctctatt ataccattta actatagcgg ctacttttct
      541 atgatactat aagattttga cgaaatcaag tatttcttta cttagaaaaa tagtcgtttt
      601 tttagtctaa ttctatttgg agaaatttac tattttcaat agctcttaaa tggtgtgttt
      661 cttgtggaga gaggctaata aggctatctt tttcaaggat atgtttctga tcaccaagga
      721 taatttcaag ttttctttcc aaaacattta ttaattttgg atgagggcat cgttcatttt
      781 tcagtccccg gctgatgatt ttaatggtat tgaataaacc ttcatcttta atcattccta
      841 acggggacat aagacttatt tttccaaatt gatattctga actttaaaaa ccattttgta
      901 agaataaatt ttgccaatca tctattttta gaggagcaac gttaacattc atagtatgtc
      961 taagttgatc gatcagcttg gcagtgtctt catcttgata agaaacatca tgtgtgagta
     1021 aacgtccacc aggttttagg acacgtaagt actcttttat ggctttctcc ttagctgaat
     1081 tgttaagcat cgttaacatt gcttcgttga taacgataaa ctattatcag gaaagggcag
     1141 tctgagtgca tttccctgaa taacttcaat aaattcttct aattgggcct ttttgacatt
     1201 ttctttggcc ttttctatta ctttactatc tagatcaagc gctgtaatgt gacaggaaaa
     1261 tgctttagcg atatcgatgg aactggtaca ttgattacaa gctacttcta gaacacgact
     1321 gtcagcacta aagtgtcctt ttttgataag ccaattcgtt gcttggacac ctcctggtct
     1381 taagcgtttt ttcctagtcg tgctaagaat tgatgtccaa tttctttatg tggcaataag
     1441 atcacctctt tcttttaata gtaatatcat tttttcttaa ttataacaca tagatgctaa
     1501 gataacaagg agtaatagtg attgaatggt ataagtgatg aattttagag agcgttttct
     1561 tttaactagg ggtgtgatgc caaaactaga atggcataga gaaccataca tctcaaaagg
     1621 tcttccttag ataaaactag ttgtttttat atctgtcaga aaaatcataa tttatttttg
     1681 atataatgat tttttgagta tttaatttat atgttataat gaaaaaaact aaaaaagagg
     1741 aatatcatgt catacgaaca agaattttta aaagactttg aagagtggtt acaatcgcaa
     1801 ataagtatca accaaatggc aatggacagt gcaaagaaag ttttagagga agataaggat
     1861 gagcgagcag cagatgcgta tattcgttat gagagtaagt tagacgctta tcgctttttg
     1921 caagggaaat ttaataacta tcataaccaa aaatctttcc atgatttgcc tgatggtctt
     1981 tttggacaac gtcattatta aaaggtttat cgctagaaag cttgtcaaat aggcatttct
     2041 atgatacatt gttatagtag tttttagata ctatgataga tttcataaat gaggagaaag
     2101 cattatacaa tggcaaagcg tattaaaccg aataaaaaaa gagagctttt gaaagctaaa
     2161 gggatcaatt ggaaggatgt tcgactatct caaactttac aagaagtcgc atcaccgaaa
     2221 aaaaaggctc ctgaagcgcc aaaggcagtc gatcttttag aaggtttaac tgctaataaa
     2281 caagatgttt taaaagaagc tggccttgtc agccttgaag cgtttgcgaa agtaagtgaa
     2341 gcagatgttt tagcactaaa aggtattggt ccagcagcta ttaaacagtt agttgataat
     2401 ggtgctgttt ttgcaaaatg atttgattca ccttgcgaaa gtagtcttat tttgctagaa
     2461 taaattaact tagaggtgtt ttgaaatccc atttgtcaca ggaagcggtc atactgagaa
     2521 atccgcacaa atgtcaaagc ttgttgctaa gtccaataat tgaaacaaaa cgagttgcgg
     2581 atgttatcat ccgaatttgg gtggtaccgc ggaaatcgta atacgaaatt cgtccctgtc
     2641 agaattgaca gggatttttt gtatttagga gtgaggagat tgaagtatat gcagattcta
     2701 gaagatttcg atggcaaagc attaccaaaa ctagaaacgg atcgacttat cttgcgtcag
     2761 cgaacggtgg gggatgtccc agccatgttt gattacgttt gcttggaaga ggttgcctat
     2821 ccagcagggc tcagtccaat agcttcttta gaagatgagt atgattattt tgaaaatcgt
     2881 tactatcaaa atctggaaaa ggcaaaatta ccatctggtt atggtattac cgttaaagga
     2941 agtgaccgga ttatcggctc ttgcgacttt aaccaccgcc atgaagacga tgttttcgag
     3001 attggctaca tgcttcatcc tgactattgg ggtcatggtt atatgacaga agctgtcgca
     3061 gccttgattg aagtcggttt taccctcctc aatctccaca aaatcgaaat ccgctgctat
     3121 gattacaata agcaaagccg gcgcgtggca gaaaaactag gcttcaccct tgaagccacc
     3181 atccgtgatc gtaaggataa tcaaggtaat cgctgtgtca atttgatata tggtttgctg
     3241 aggagtgagt gggagtaatt attctactct tcatagaagt tactagatag actaatttgg
     3301 ctgtgcaggg tacttagcta actcatcaat aatatcgtag atatggagga gacaaatgat
     3361 taagatactt ggagaaatcg tagataacca gctacctgtt gtggaaacga atcgtctgtt
     3421 gttgcgtcaa cgcaagttag aagatgctaa ggagatattt gaatttgtca aacttgatga
     3481 ggtgacttat ccagcaggtt ttccggctgt taaaagcttg gaagaagaga taacctatat
     3541 tcaagagatt tatccaacta atcttgaaaa agaaaagtta ccctctggtt atgctattac
     3601 tctaaaaggc gatgataagg taattggttc agtagatttt aaccatcgcc acgaagacga
     3661 catttttgaa attggctacc tgctccaccc tgattattgg ggacaaggca ttgtgccaga
     3721 agcagcttca gtccttgtgg agataggttt cacactgcta ggactacata agattgagct
     3781 gggatgctat gactataata agcaaagtca ggcggttgcg cgcaaattag gttttacttt
     3841 agaggctaat attcgtgatc gaagagatgc ccaggggcaa cgttgtggcg atatgcgctt
     3901 tggtttactg cgaagcgagt gggagaaaaa aagaagatga atattattat catcggagca
     3961 caagcttctg ggaaaatgac aattggtcag gaaatcgcaa aacagacagg catgacctta
     4021 ttccttaacc atgattctat agattttgtt ttacgcttca tgccatggtc tccagatagt
     4081 attgctctaa cggagtctat tcgctttaaa ttttttgaaa cgtttgctaa gactggtcaa
     4141 gaaatgattt ttactattgt aattgatttc aatgattcaa gagatgtagc atttctagaa
     4201 aagattcaaa cagtttttca atctcacaac caagaggtat tatttgtaga attggaaaca
     4261 gagctaagtg agcgtctcaa acgaaatcgc acagaaaatc acctcaagca taagccttca
     4321 aaacgtgata tcaaatggtc agagaacgat atttgttcaa ccatggatta cgctatcttt
     4381 aatcctgaag tagccccaga agcgcttaca tattattata aaatcaataa tacttgctta
     4441 actgctactg aaacagcata tcttattatt caaaaaataa atcaaatcaa ggagaaaaac
     4501 taatgtcaaa agaactatca ccaaaataca atcctgccga ggttgaggca ggccgttatc
     4561 aaacttggct tgaccaagat gttttcaagc catcagggga tgctgaggct aagccttatt
     4621 ctattgtgat tccaccacca aacgttacgg gtaaattgca cttgggtcac gcttgggata
     4681 caactctaca agatatcatt attcgtcaaa agcgtatgca gggctttgat acactctggc
     4741 ttccaggtat ggaccacgct ggtatagcca ctcaagctaa agttgaggag cgccttcgtg
     4801 agcaaggcat ttcacgttat gatcttggtc gtgaaaaatt cctagataaa gtttgggaat
     4861 ggaaagacga gtatgcagca actattaaat ctcagtgggg gaaaatgggt ctttcggttg
     4921 attatagccg tgagcgtttt acccttgatg agggactttc aaaagcggta agaaaagtct
     4981 ttgttgattt gtacaataaa ggttggatct atcgtgggga gttcatcatt aactgggatc
     5041 cagcagctcg tacagccctt tcagatattg aagttattca caaggatgtt gagggtgctt
     5101 tctatcatat gaattatatg ctggaagatg gctcacgcgc cctagaagta gcaacaactc
     5161 gtcctgagac tatgtttgga gatgtggctg tagcggtaaa tccagaagat ccacgttaca
     5221 aggatttgat tggtcaaaat gttatccttc ctatcatcaa taaacctatt ccaatcgtcg
     5281 ctgatgaaca cgctgatcca gaatttggaa caggtgtggt taagattacg ccggctcacg
     5341 accctaatga cttcgctgta ggtcaacgcc acaacttacc acaagttaac gttatgaacg
     5401 atgatgggac tatgaatgaa cttgcggatg aatttaatgg tatggatcgt ttcgaagctc
     5461 gtaaggcagt agtcgctaaa ttagaatcgt taggaaactt agtaaaaatt gaaaaaatga
     5521 ctcattctgt tggtcattca gaacgaacag gcgttgtggt tgaaccacgt ttatctactc
     5581 aatggttcgt caagatggat caattggcta agaatgccat tgccaaccaa gatacagaag
     5641 acaaggtaga gttttatcca ccacgattta acgatacttt tatgagctgg atggaaaatg
     5701 tccatgactg ggttatctct cgtcaattat ggtggggaca ccaaattcct gcatggtaca
     5761 acgtcaatgg tgagatgtat gttggagaag atgccccaga gggtgacgga tggacacagg
     5821 atgaagacgt tctagacaca tggtttagtt ctgctttatg gccgttttca acaatgggat
     5881 ggccagacac cgaagcagct gacttcaaac gctacttccc aacgtcaacc ttggtaactg
     5941 gatacgatat catcttcttc tgggtatcgc gcatgatttt ccaatctctt gaatttacag
     6001 gacgccaacc attctcaaat gttcttattc atggactcat ccgtgatgaa gaaggacgta
     6061 aaatgtctaa atcacttggc aatgggattg atccgatgga tgtcattgag aaatatggtg
     6121 cggatgctct tcgttggttc ctctcaaatg gttcagcccc tggtcaagat gtgcgttttt
     6181 catacgaaaa aatggatgct tcgtggaatt tcattaataa gatttggaac atttcacgct
     6241 atatccttat gaataacgaa ggtttaacgt tggatcaagc cagagaaaat gtggaaaaag
     6301 ttgttaatag tcaagttgga aatgtaacag accgttggat tctccacaat ttaaatgaga
     6361 ctgtgggtaa agttactgaa aactttgaca agtttgaatt tggtgttgct ggtcacatcc
     6421 tttacaactt tatctgggaa gagtttgcta attggtacgt tgagttaaca aaagaggttc
     6481 tttatagtga caatgaagat gagaaagttg ttacacgttc tgttcttctt tataccttag
     6541 atcaaatctt gcgtcttctt catccaatca tgccattcgt gacagaagaa atctttggtc
     6601 agtatgcaga tagctcaatc gtaacagctg cttaccctac ggttaaccca gcctttgaaa
     6661 atgctgctgc ccacaaggga gttgaaagct tgaaggactt gattcgttcc gtgcgtaata
     6721 gccgagcaga ggtcaatgtt gctccatcta aaccaatcac tatcttggtt aagacaagtg
     6781 acagcgaact cgaaagcttc ttcaaagaca atagtaacta catcaaacgt ttcacaaacc
     6841 cagaaacact tgaaatcagc tcagctattg ccacaccaga acttgccatg tcatcagtca
     6901 tcacaggagc tgaaatcttc ctcccacttg ctgacctcct caacgtagaa gaagagttag
     6961 cacgacttga aaaagaacta gccaaatggc agaaagaact ggacatggta ggcaaaaaac
     7021 tcggcaacga acgcttcctc gccaacgcca aaccagaagt tgtccaaaaa gaaaaagaca
     7081 aacaagccga ctaccaagct aaatacgacg caacccaaga acggattgta gagatgcaaa
     7141 aacttgttaa atagttaaaa tcatccatta cttccgcaga gtcctgccgc tagaccatca
     7201 gtctaagcga cagggctttt tgctatattc aaagattgtg aaaaattaat atatatgatg
     7261 cctacttctt gcgtctgaaa tacaagaagt gaaaatgatt tggccataaa gaaaaacagg
     7321 cagaaaccca aatgattaat gcctatttct tatatttttt cgtcaattat tttataaata
     7381 aaattgaggt acaaaccagt ttttaaaatg ataaggtgaa tcttcttcaa tatagatgtc
     7441 tgtatataaa aaggtatagc tatccatatc atcaaaaata tcaaaaggcc actcatcgtt
     7501 ataatctaat aattccttaa gttgtagatg ggagttttcg tccagagatt caaaataaag
     7561 aatggattct tttgcaatta aatagagata aatttcctct attacactat gcgctgtata
     7621 agatgaacca actttatttc ttgatgtata ttgag
//
LOCUS       DATRNX010000086         7569 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRNX010000086 DATRNX010000000
VERSION     DATRNX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            103..2340
                     /gene="metE"
                     /locus_tag="VBC81_001551"
     CDS             103..2340
                     /gene="metE"
                     /locus_tag="VBC81_001551"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6912582.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2385..4229
                     /locus_tag="VBC81_001552"
     CDS             2385..4229
                     /locus_tag="VBC81_001552"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6912583.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4576..5283
                     /locus_tag="VBC81_001553"
     CDS             4576..5283
                     /locus_tag="VBC81_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6912584.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5448..6533)
                     /locus_tag="VBC81_001554"
     CDS             complement(5448..6533)
                     /locus_tag="VBC81_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6912585.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6734..7273)
                     /locus_tag="VBC81_001555"
     CDS             complement(6734..7273)
                     /locus_tag="VBC81_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6912586.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         2424 a         1237 c         1454 g         2454 t
ORIGIN      
        1 atataaaata ttaaaagcta ttgatattat agttttcatg gactaaaata attcataaaa
       61 ctaaaatggt tagttattag ctataacttg atggaggaaa ttatggtaaa agtttcaaat
      121 ttagggtatc cacgtcttgg tgaacagcgc gaatggaagc aagcgatcga agctttctgg
      181 gcagggaatc ttgaacaaaa agatttagaa aaacaactaa aacaattacg tatcaatcat
      241 ttaaagaaac aaaaagaggc aggtattgac cttattccag tgggggattt ttcttgttat
      301 gatcatgttt tggatttgtc atttcaattc aatgtaatcc caaagcgttt cgatgagtat
      361 gagaggaatt tagaccttta ttttgctatt gcaagaggtg acaaagataa tgtcgcatca
      421 tctatgaaaa agtggtttaa taccaactac cactacatag tcccagaatg ggaggttgag
      481 actaaacctc acttgcagaa taattactta cttgatcttt atctagaagc tagggaagta
      541 gttggtgata aagcaaagcc ggttatcact ggtccaataa cctatgtttc cttatcatca
      601 ggaattgtcg actttgaagc gactgttcag cggttattac cactttataa gcaggtcttt
      661 caagatctga tagatgcagg cgccacctat attcagattg atgagccgat atttgtaact
      721 gatgaaggtg aacttttagt agatatagct aagtctgtct atgatttttt tgcaagagaa
      781 gtaccacaag cccacttcat ctttcaaacc tactttgaat cagcagtctg tttagataaa
      841 ctctctaagc tgccagtaac gggatttggc cttgatttta tacatggtag ggcggaaaat
      901 gtagctgctg ttaagcaagg tctattccgc gaaaaagaat tatttgcagg aattgttaat
      961 ggtcgaaata tctgggcagt aaatttagaa gaaacgttgg ctttattgga agagataggt
     1021 ccctttgtta aacgtttgac ccttcaacct tcttcaagtc ttttacatgt accggtgacg
     1081 actaaatacg aaacacattt agaccctgtg ttaaagaatg gcttatcatt tgctgatgaa
     1141 aaactaaagg aattagaact attagcgagt gcttttgatg gtaataaaac aaagggatat
     1201 cacgaagctt tatctcgttt ttcagctctt caagctgctg attttcgtca tgtagcgttg
     1261 gaatcattag cagaagtaag gcttgaacga agtccgtata aattacgcca agctttgcaa
     1321 gctgaaaaat tacagttacc gattttacca acaacaacta ttgggtcctt tcctcaatca
     1381 cctgaaatta ggaagaaacg ccttgcttgg aaaagagaaa atctatctga ctcagattat
     1441 aaagatttca taaaaactga aattagaaga tggattgcta ttcaagaaga tcttgatctt
     1501 gatgtgttag tacatggcga atttgagcgc gtggatatgg ttgaattttt tggtcaaaag
     1561 ttagctggtt ttacgacaac caaattaggc tgggtacagt cttatggttc aagggcggtc
     1621 aaaccaccta tcatttatgg tgatatcaaa catattcaac ccttaagcct tgaagaaacg
     1681 gtttatgccc aaagcttgac taagaaacct gttaaaggca tgttgacagg tcctattact
     1741 ataacgaact ggtcatttga gcgagatgat atcagccgat ctgatctttt taatcaaatt
     1801 gctttggcta taaaagatga gattcaactt ttggaacaat caggtattgc tattatacaa
     1861 gtggatgaag cagcccttcg agaaggctta cccttacgcc agcaaaagca acaggcttac
     1921 ttagatgatg ctgttgcggc ctttaaaatt gcaacttcat ctgtgaaaga tgagacacaa
     1981 attcatacac atatgtgtta ttcaaaattt gacgaaatta ttgattctat ccgttcacta
     2041 gatgcagatg ttatttctat tgaaacgagt agaagtcatg gggacatcat tgaaagtttt
     2101 gaaacagcag tttatcctct aggaattggt ctgggtgttt atgatattca ttctcctcgc
     2161 atacctacta aggaagaaat tattgtcaat attcaacgat cactaaaatg tctatcaaaa
     2221 gagcaatttt gggtaaaccc tgactgtggc ttaaaaacac gtcgtgaagc agaaacaatt
     2281 gctgccttgg aggttcttgt ttcagctacc aaagaggttc gtcagcaatt agataattaa
     2341 taggttaatc agtaaaggat tgccatgagg gaggagtaaa gaagatgtcc aagtttttag
     2401 agaaattaaa aacagatatt ctagtagctg acggtgcaat gggaactctc ctttatactt
     2461 atgggctaga tacttgtcat gaatcctaca atgtaacaca tccagaaaag gttctagcca
     2521 ttcatcaagc ttatattgaa gcgggtgcag atgttattca gacgaatacc tatggggctc
     2581 aacgtcatcg attaaagaac tacggtttgg aagatcaggt tgtctctatc aatcaagcag
     2641 cagttaatat agctcatcaa gcgactttgg gtaaagaaac atttatttta ggtactgttg
     2701 gaggttttcg ttctcaaaga caatgcgatt taacattgga taatattgta gaagaaactt
     2761 tggaacaagt tgaggcttta ttggtgacag gacagctaga cggattattg tttgaaactt
     2821 attatgatat tgaggagata acgacagttt taaaaattgt gagggagatg actgatctac
     2881 caattattac taatatttct cttcatgaag caggggtaac atcaaatgga aagcctattg
     2941 tagaggcgct cagccaatta gttatgctag gtgcagatgt gattggctta aattgtcacc
     3001 ttggacccta tcatatgatc caatcattga agcaagttcc tttatttgcc cagtcttatt
     3061 tgtcagttta tcctaatgca ggtcagttat ctttagatgg tgagaatagt caataccagt
     3121 ttagtcagaa ttcagagtat tttggaaaat ctgcagaact tctcgtagca gaaggagtta
     3181 ggcttattgg tggttgttgt gggacaactc cagatcatat tagagctgtg aaacgatcta
     3241 ttagaggatt gaaacctatt gaacgaaagg ttgttactcc tataatacct gtaaaagatt
     3301 ttgttaggcg tatcagacga acagatactc tagtggataa ggtaaaaaaa gaagtaacaa
     3361 ttatcgcaga attagatcct ccaaaacatt tagatatcgt tcagtttcaa aaggctatac
     3421 gagcgattga ccaaaaaggt attgcagcaa ttactttagc agataattca ctatctaata
     3481 cacgtatttg taatttaagt atagctagct tgcttaaaga cgaaattagt acaccctttt
     3541 tactacatat agcttgtcgt gaccataatt taattggttt acagtctaga ctgttgggca
     3601 tggaattatt aggttttaac catattttag ccataacagg agatccgact aaacttggtg
     3661 atttcccagg agcgactagt gtttatgatg tgacttcctt taaactcctt tctctgatta
     3721 aacaacttaa tcaaggttta tcttatagtg gtgctagctt gagacgtcca acagatttta
     3781 cagttgctgc tgcctttaat cctaatgtga aaaaccttac tcgaaccgta aaattgattg
     3841 agaaaaaagt agcttctggt gctgattatt ttatgacaca accaatattt gatcactctg
     3901 tgttaaggga attagctgat cttactaaaa cagttgaaca accctttttt atcggaatta
     3961 tgccaataac aagttataat aacgctgttt ttctacacaa tgaagttcct ggtattaaac
     4021 tttctgagtc atttttatct gctcttgaaa aggtaaagga cgacaaagaa gcttgtttaa
     4081 ctttggcctt aaacgagagt aagtccttga ttgatgaagc cttgaaccat tttaatggta
     4141 tctatttgat tactcccttt ttacgctatg atctgactct tgagttaatt gactatatac
     4201 aaaaaaagca agtaagaaag tcctcttaac tttcttactt tttagttggt cgtttattat
     4261 tataataaaa gttacgtacc aaaacagagg ttaaactaga tttttaagcc actttctata
     4321 ctaagataaa ttagttaatt taagttttct ttaagtttat agacttagaa taaagcgtta
     4381 ttttgatggg tagcataaaa tcaaggcata atatagatgt cagcgatttt tttagactaa
     4441 cgaaataacg atgtaaagct taataggggt gtatcgaata ctcacttata tagtaccaaa
     4501 agcctatagc ctgttggcta gcttgaccgt attcaaatgt tgaataaatc ttggttgaga
     4561 aagggaggta taaaaatggt atgtgtcgat tttattgatc aaaaagttta tagtgaagca
     4621 gaaggtctat ttgtttccga tttagactta ggcattaaag gtttaataag agcacgttat
     4681 cctttcgctc tggatagtga ctttatttcg tatgggaatt taagtcatta ttgtatgaat
     4741 tatcttgatg aaattgttca gcgagcgaat cagaaaaatg agaagataaa atataatgtg
     4801 actaccttga tgaaagaaga agagaagccc ttcaacgttg aagagcgttt gaacaagcaa
     4861 gcaactatcg gtcaacgaat tgctgatgat gttgcacgtt ttggcggatc ttggactttt
     4921 attatcgttt ttgtatccat tatggctatt tggatgttgg ttaatatcat gaagcctttt
     4981 ggtattcaat ttgatcctta cccatttatc ctgttaaact tagctttatc aacgattgct
     5041 gcgatccaag caccacttat tatgatgagt caaaatcgtg ctgctgatta tgatcgtttg
     5101 caagctcgaa atgactttaa tgtcaataaa acctctgagt tagaaattag attattgcac
     5161 gaaaaaattg accatatggt acaacaagat cagtttgaat tgttagagat tcaaaagtta
     5221 cagacagaaa tgctagtatc attgggaaat cagttagcac aattgaaaca actacaaaaa
     5281 taatctttta gcagttattt aaaaatactg atacaatact aagaatttaa attaatactg
     5341 caacctccta taatggagag cagtattttt actttattaa taacaaaaac cccttagatg
     5401 atgtgtctaa gggatttttg tatcggaata atcgagttgg tgaattatta ggctagtgtc
     5461 tgtccgaaag cgtctgctgc taaaatagct tgagcaaaca ttttgggatc gaaaggggta
     5521 tttttgtatt cattagtagt cattgcaatt tcaacaattt ttggaatatc tttttctgat
     5581 aaagagacgt cagctagagt tgttggcaaa cctagtgact tattaaaacg agctactttt
     5641 tcaagttctt ctagttcgtt gaaataagca tgtaaaacta acacaccaaa cgcaactaca
     5701 acgccatgta agaattcacc ttgtctttga atagcggttg taccatagta aaatgcatgc
     5761 gcgtgacaag aattgtagta gaaatcaggt tgattaacta aattagaagc atagccagtt
     5821 gagattaaga tatcaagcgc aatttcttca actgcacgcg aagctaaatt agcttcaacg
     5881 tcttttagac cttgttcacc aaattgataa aaagcttcct ttgaagacag agcgactgct
     5941 tgtcctaaca ctgcagtatg tggtagttta ttggtcttag cctctaaggt agcacgttct
     6001 acttcagggg cttttgaaat accgtcccca ataccagccc aaaaatattt gcttggtgcc
     6061 tgtgcaatga tccgtgtatt gataaaaata tgaagtggag actcgggata gccgtatctt
     6121 aagaaagaat ggtcatcatt atatacaact gcaatggcag tacctgcgga gcaattagaa
     6181 caaatagttg ggatagtaaa gctattttta cctaactctt ttgctaccat tttagctgtg
     6241 tctaaagctt ttccaccgcc aaaacctaag atagcatcag cagcttgaac ttgtgggttg
     6301 gctactaact tgtcaatgtt agattgtgtt gaatccgttc catagacaaa gcttcctgtg
     6361 actttaatat ttgcttcctc taaaatagag cgaacctcgg cttctgagct gtctagggca
     6421 cgtttgccac ccacgaaagc aatgctttga atacggtaag ttgccaaaac gctaggaatt
     6481 tctttgaagc aatcttctcc gtagctataa tttgccatta catttgttgt cattcagtgt
     6541 cctcctctat tccaaaggtc gattagagac agctattttc taaaagtgaa tagttttttg
     6601 tctcttgacc aagtgattat catattatag cagaatattc agaaaattgg aacatgaaaa
     6661 ataatttaat tttcaagaat aagaatagaa aaggtaaagc gagatatacg agtaaatact
     6721 gtcagtataa taattataag ctcttgagag ggtctgtatt ggatgcaaca gggagttctt
     6781 ttaggtattg atcaagatcg cgttggtgtt ttagcacctt tattttttga gggtagtcct
     6841 caacaacttg tttatagttt gatttttgtt tgtcagagcg accgtcttta agaatccaac
     6901 tgataaattc aaaatctaat ttttccgggc atccattggc catatcaggt ctagtcttac
     6961 cacgaaactt acagtaacgt ttgaatgctc tccacaagca gttgaagcga ttaaagtcaa
     7021 aaaaaataat ctcatctgca tctgctagac gttcttgata tagcagtttt ttgtagttac
     7081 cttctataat ccatgttctt ttttctaaca tgctgctgac atcgtcaatc atgtcatcat
     7141 attttctctc ttcccaattt ggggcgaagt gaatactatc aaggtgtaat acattgcaat
     7201 tatagtaatt tcccaatttt ctcgctaaag tggacttacc tgacccacta tatccgatta
     7261 ttgctatttt catatactac ctccgttaaa ttgtttactt gaagttaatg ctgtacttag
     7321 gatttactta ttttataagg gttattcact tttttagatt aatatatttt tgatataagt
     7381 tatccataca catggtaaca taattaccag taaatgtaat ctagatatca atactatggt
     7441 tgacgatatt gttaactttt gggttcaaaa aattggggta aaaatcatcg gagggaactg
     7501 ccaaacaaac tcacagttta aaatactaag taataaaaag tcttgaattt attatgttta
     7561 aggcttttt
//
LOCUS       DATRNX010000087         7569 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRNX010000087 DATRNX010000000
VERSION     DATRNX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            163..834
                     /locus_tag="VBC81_001556"
     CDS             163..834
                     /locus_tag="VBC81_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6912587.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            919..1590
                     /locus_tag="VBC81_001557"
     CDS             919..1590
                     /locus_tag="VBC81_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912588.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            1574..2938
                     /locus_tag="VBC81_001558"
     CDS             1574..2938
                     /locus_tag="VBC81_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6912589.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(3049..4602)
                     /locus_tag="VBC81_001559"
     CDS             complement(3049..4602)
                     /locus_tag="VBC81_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6912590.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(4696..5622)
                     /gene="arcC"
                     /locus_tag="VBC81_001560"
     CDS             complement(4696..5622)
                     /gene="arcC"
                     /locus_tag="VBC81_001560"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6912591.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            complement(5634..6632)
                     /gene="argF"
                     /locus_tag="VBC81_001561"
     CDS             complement(5634..6632)
                     /gene="argF"
                     /locus_tag="VBC81_001561"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6912592.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            6735..>7569
                     /locus_tag="VBC81_001562"
     CDS             6735..>7569
                     /locus_tag="VBC81_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6912593.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DI"
BASE COUNT         2581 a         1418 c         1178 g         2392 t
ORIGIN      
        1 ctcttacctt caggtagtta gtcattcttt taaaactcac ttttttataa ctgaaaatat
       61 tgttcttaat tttaaaaatt cattttagat tcattttacg actatatact cagaagtaac
      121 aaacctaatc caaggtttga aaaaagaaag aaggaagtca gtatgacaaa ctataaaaac
      181 aactttaaaa aattcatgag caataaatta tacgtagggt tagcttccat tacgctactt
      241 ggtattgggg gagctatagg agtaagtgga gctgcattag cgcataacca tcactcagaa
      301 cacatgtctt ttgaacataa gcatgaagga cacgataaac atcacgatca tagagaaaaa
      361 tactcactaa aaacattaaa gaatatctcg acaaaaatcg attataagac tgccttagaa
      421 actgccaaaa aagaagctcg ctcaggcttt atttcacaaa ttgaattaaa taccaaaggt
      481 gatacacctt tttatgaaat caaagttatt gataataaaa tagctaaaaa atataaaatt
      541 gatgctaatt ctgggaagat cctttcaaca acatcagata atcaactcaa ttcaaaagat
      601 gcccaattaa aacaaccaag taaagatatt cagactgcat tacaagctat tcaagcaaaa
      661 tataaaaatg caaaaataaa agaaatttct cttgcgaata acaaatcaaa taagctagtt
      721 tataaagtaa aactacttga tggaaatact cctaagattg cccaatttga tagtgataaa
      781 gcatcattta taaaggcaaa taagcttaat acaactcata aaaatcacga ctaatactag
      841 tgtattaaag ggaataggac ctcagtactc cattcccttt tgtgatataa tggaaaaaag
      901 ccatacggag gccaaaaaat gaaaatcctt gttgttgaag atgagtttga cttgaaccgt
      961 agtattgtaa aacttttaaa aaagcaacac tacagtgttg acagtgcttc aaatggtgaa
     1021 gaagccttac aatttgtttc agtggcagaa tatgatgtga ttatcttaga tgtaatgatg
     1081 ccaaaaatgg acggcttcac ctttttaaaa ctgcttcgca acaaaggaag tcaagtatct
     1141 attcttatgt tgactgctcg agatgccgtt gaagatcgta tcgcaggtct agattttggt
     1201 gcggatgatt accttgtaaa accatttgaa tttggagaac tcatggcccg aattcgagct
     1261 atgttacgac gtacaaatag acaagtatct tctgatgata ttcaaattca agatataaca
     1321 atcaatttat ctacaaagca agtttggaga aacgacaatt tgattgattt aacagctaag
     1381 gaatacgaag tccttgagta tttggcacgt cacagagacc aagtcctctc tcgtcatcaa
     1441 attcgtgaac acgtttggga ttatgattat tatggagagt ccaatattat tgatgtcctt
     1501 atcaaaaacc ttcgtcgaaa actagataac aatcgagatg gatcactaat aaaaactaaa
     1561 cgaggtttag gatatgttat tccaaagtaa aataaattac cctaaaaagt ctagcatcat
     1621 cactcaaatt accctctggt acagctcttt tatttttatc cttgttatcg gtgtattaat
     1681 tggttcattt tttatttcaa aaagcattgc tgaaaacaaa tctaaaaaaa atttagaagc
     1741 taaagctgtg caaatgagtc aagcgttagc taaaggtcat cggtatgagg cttttgaaga
     1801 tggaatattt tattcagtct atgaccaaaa tggaaaagtt atctattctg gttttccaaa
     1861 aggatttaaa agggatttag accatcaaca taaacataaa aagaaattat ctctattttc
     1921 catggagaat cgtacctttc agtatgttga tattccaatt tctgggaaaa atcagtggtt
     1981 acgtgctatc cgtactgttg acagactgga taagcaatta actgaattgt tatttagttt
     2041 gggtatcgtt cttcctctta tgttgattat aataactgtt ggtggctatt taattttaaa
     2101 gcgcgctttt agaccgattc aagaaattac tgaaaccgct caatttatta cccaaaatga
     2161 agactatact aagcgaatta tcactaaaaa taacgaaaat gaattaactg agctagcagc
     2221 tgttatcaat actatgttgg cttctattga atcatcattt gttcgggaaa aacaatttaa
     2281 caatgatgtt tctcatgaac tacggactcc tgtaacagtt atactttctg aaagtgaata
     2341 cggaaaaaac tacgcagaaa atttaagtga agcaaaagaa tcctttgagg ttatacaccg
     2401 tcagtcacta tcaatgaaaa aattagttga gcaacttcta gaattgacta aggcagaaaa
     2461 tccattatca attcagttag aacctcttaa tttctcaatt atgatgaagc aactagtatc
     2521 cgattcgagt agattattag ataatacccc tatacacctt gactctcaaa tcgaagatga
     2581 cctttggatt ataggtcaac aaacactact caaacggctt tttgataatc tattttcaaa
     2641 tgctattaaa tttacaaaca atcatatttc tattagtcta agacagtccg ataatcaaat
     2701 tgtcttctct attaaagata atggtctagg tatctcagtt gatgaccaaa gtaaaatttg
     2761 gaaccgtttc tatcaagtgg actctgctcg aaccaaggat agtcagtctg gaattggttt
     2821 agggctatct ttagtaaaac aaatcgctac aatacaccgt gctaaaatat gggttgattc
     2881 taaacctgat gatggcagtc agtttacgct cacctttcca aaattgaaga aagactagag
     2941 aaaggaaata aaaaaaaacg atagattaca cctatcgttt tttttattta acttaaactc
     3001 aatttattat atctcttcag atatttttga gactaatagc tgaaatcttt aaagtattaa
     3061 aacttgtaga actaacaaac cgccaccaaa tattaggagg aataaaacaa ccggccacac
     3121 aaattttagc caatgggagt acttcatatc taacatctgc aaggttgcca taactagtcc
     3181 agtaggtgct aaaaacaaca ttgcatactg cccaaattga taagccatta ctactactga
     3241 acgaggaatc ccaacagtat ctgcaagagg tgctagaatt ggcattgaaa gtactgctaa
     3301 acccgacgag gatggaacaa caaatcctaa cacaaagtag ataaacatca taacaatgat
     3361 aaagacaggg ccactaacat gtgaagctaa ttttgaagct gtgtaaagca tcgtatctga
     3421 tatataacct tggctcaaaa taatattgat accacgcgct aaaccaatga ttaatgaaac
     3481 cccaacaagg cttgatgcac cattaacaaa ctcatcaaca acatcccttt ctccaatacc
     3541 atttgcccca gttgctgtta aaaacataat aataattgta atagctaaaa aggaagaagc
     3601 catggttgga aaccaccatc cctgtgacat tactccccaa accatcaaag gaaatgaaat
     3661 aacaaataaa cttaaaataa ttttttgtct aatagtaaag agtgagggtg tatggttagt
     3721 tgtcatgccc catttagcat taaattctac tctatcttca taggaatatg aaaactctgg
     3781 attcgctttt atttttttag cataccaatg tagatacacc acaacaaaaa tagcacctgc
     3841 aatacaccct gctgtacgcc atgataagcc ttctgtgaaa ctaataccag ctgcatttga
     3901 agcaataaca gaagaaaacg gattaattgt tgaaaatgat gtcccaacag aacttgctaa
     3961 gaaaatagca cctacacaaa tgatagaatc ataacccatg gctaaaaaga ttggaactaa
     4021 aataggataa aaagcaactg cttcttcttc aataccacat agagtaccgc ctaaaaccat
     4081 caataaggaa actaaaaata tcaataaaaa ctctctacct ttggttttct ttgttaaagc
     4141 tagaagacca gactcaaaag cgccactctt tctaactact cctatcatgc ctccaagtac
     4201 catgataaat accataatat caacagcttc aatggttcca tttaccatac tagtcgtcac
     4261 ggagccaatg cctgctggat tttgcttcaa acgtttgtaa gtatttggaa ctgaaactgg
     4321 cttactaata gcaccagaag taaattcttt aattttaatt ttgacattca tcttatccaa
     4381 ctgtgtttgc gttgctggaa catgaactgt cttaccattt ggatctgtta ctactagttt
     4441 ggatgatgct gtatcaaatt gtaattttga ataggaccca gcaggtatcc accatgtagc
     4501 caaaaccgaa aagattgtta aaatgaataa tattgtaaag gcacctggca ttcgaaaacc
     4561 tttactcctt ttgttttcac tgtttgtcat tttttctggc atgacacgcc tcctctataa
     4621 taaaaataga ttgacttcat cagaaaaagt gcaaagatga aggttaaata atcatttctt
     4681 tgcacttatc tgagattact tagttatacg tgttcctaat ttcccatcta atgcatcttc
     4741 taaaccattt aatgaggtga ttaaggctat tcctttcgga ttgtgatcta agaaactcat
     4801 acaggctatt actttaggta acatacttcc ttttgcaaac tcaccttggt taacgtaatc
     4861 aatcatttga ttctcattaa cctctgttaa agctttttga ttttctttac caaattgagt
     4921 ataaacataa tcaactgcag tgagaataat caattgatcc gcagctagtt ctcctgctaa
     4981 cagtgcacta gatttgtctt tatcaataac agctgcaata ccttcataac ctcccttatt
     5041 aatgacaggg atgcctccac ctccacctgc gataactagg gtatcatttt caataagtgt
     5101 tttaatgcta ttaatttcga tgattttttt aggttcaggt gaagcaacaa cacgacgata
     5161 ccctcgtcca gcgtcttcta cgaaagtata ccctttctct atagcaatct tttctgatgt
     5221 ctccttatca taaaaggtgc ctataggctt ggtaggtgcg ctaaatgctt ggtctgattc
     5281 atccacctca atttgggtaa taattgtagc aacttcctta tttattccct cttgacgtaa
     5341 ttcatttaaa aggctctgtt ggagatgata gcctatgtat ccttggctca ttgctccaca
     5401 ttctggaaaa ggaaaattag ttccttgacc actctcagcc gcaaaattca ttcctaaatt
     5461 aatggcacca acttgtggtc cattaccatg acttacaaca atttcgtgtc ccttttttat
     5521 caaggccacc aatgattttg cagtatgttt tactaatctt aattgttctt ctggagaatt
     5581 tcctaaggca tttcctccta gtgcaacaac aatttttgcc atgaaaattc ctcctaatca
     5641 cctaaagttg ctaccataac tgctttaata gtatgcatac gattttcagc ttcttggaaa
     5701 acaactgagt gagggccttc aaaaacttca tctgatactt ccatttcctt caaaccatat
     5761 ttttcataaa tgtcgtatcc aactttagta tctatattat gaaatgaggg caagcaatgc
     5821 tcgaaaataa cgtttggatt ttctgtcata ttaagcattt cttgggtaat acgatagggt
     5881 tctaaaagag ctattctttc tttccaaact tcatccggtt ctcccattga aacccaaaca
     5941 tctgtatata ggacgtctga atctctgacg ccttctgcaa tatcagcagt gatttcaata
     6001 gaagcaccag tagttttcgc aatttcttga catttagaga gtagttcagg atctggctct
     6061 aattctttag gacaaaccaa atgataagtc atgcctacaa tagaagcacc gatcatcaag
     6121 gcattagcga cattgttacg tccatcaccc acatacgtaa agcgaatatc tttatagggc
     6181 ttatgtaagc attccttagc ggtcagaaaa tcggctagta cttgcgttgg atgatcagca
     6241 tcggttaaac cattccacac aggaactcct gaaaattcgg ctaaagtctc aactgtttct
     6301 tgagaaaatc cacgatactc aataccatca tacataccac ctagcacacg cgcagtgtct
     6361 tttgaggtct cttttttacc catctgactg cctgttggac ctaaataagt aacatgagct
     6421 ccttgatcgt gagcagcaac ttcaaaagcg caacgtgtcc gtgtagaatc tttttcaaaa
     6481 atcaaagcga tatttttacc aacaagtttt tgttgttcaa ttcctgcgta tttagcacgt
     6541 tttaaatctt ctgaaagttt taaaagaaat tcaacttctg ctgtactaaa atccaataaa
     6601 gtaagaaaac tacggttacg taaatttttc attgcttatt gcctcctttt tttttatact
     6661 cttattgtag atagcgcttt caaaaataat caaaattcat caaaaatctt caaaaaaact
     6721 ttaaggacat ttatatgcta gatatattat cagaaaaaca tctagaaaga aggcagcgta
     6781 ttattatctc tgcaatagct atcgccttag ctgctcaaat caatatctca atacttgctg
     6841 atgggtttat tatgacctta tctttgttca ttttgcctgt ctttttatat tttaatgacg
     6901 acattaatcc aattcttctc tgtctaggaa ttacttttgc ttctcctatc ttcagaggaa
     6961 tcatattaag tatcgctggc gaggctgaga ttcatcaaat aattgaattc gttttaactg
     7021 atatggcgtt ttatatttgt tatggcatca ccttttatac tatttattgg catcgttcct
     7081 accgtaataa aggtactttt ttcttttcta tcattatttg tgattatttt gccaaccttg
     7141 tagaaattag ttttctcata aagtttaaca attatactat aactatattc gctactttat
     7201 ttgccatagc acttttgcga gcttttatta gttgcgctgt ggcttatact tatagttatc
     7261 tatcacttct cttacaaaaa gacgatcacg aacgtcgata ctattatttt atgtggtcaa
     7321 cttcagccgt taaaagcgag gtttatttta tgcaaaaaaa tatcattgaa attgaaaaca
     7381 tcatgaaaaa tgcttattta ttagataaag agttatccaa atatcaccta cctaaagaat
     7441 accaacacct ctctctagat atttcgcggg acgttcacga agttaagaaa gattaccaaa
     7501 atattattaa aggcttagga acctatttta gtgtaaaaaa tgaatcaact atggcattga
     7561 aagatatct
//
LOCUS       DATRNX010000088         7537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRNX010000088 DATRNX010000000
VERSION     DATRNX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(63..1259)
                     /locus_tag="VBC81_001563"
     CDS             complement(63..1259)
                     /locus_tag="VBC81_001563"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6912594.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1444..2379
                     /locus_tag="VBC81_001564"
     CDS             1444..2379
                     /locus_tag="VBC81_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6912595.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2360..2554)
                     /locus_tag="VBC81_001565"
     CDS             complement(2360..2554)
                     /locus_tag="VBC81_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6912596.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2643..4307)
                     /gene="dnaX"
                     /locus_tag="VBC81_001566"
     CDS             complement(2643..4307)
                     /gene="dnaX"
                     /locus_tag="VBC81_001566"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6912597.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4307..4804)
                     /locus_tag="VBC81_001567"
     CDS             complement(4307..4804)
                     /locus_tag="VBC81_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6912598.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4891..5520)
                     /gene="udk"
                     /locus_tag="VBC81_001568"
     CDS             complement(4891..5520)
                     /gene="udk"
                     /locus_tag="VBC81_001568"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6912599.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            5665..6747
                     /locus_tag="VBC81_001569"
     CDS             5665..6747
                     /locus_tag="VBC81_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6912600.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHNYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(6779..>7537)
                     /locus_tag="VBC81_001570"
     CDS             complement(6779..>7537)
                     /locus_tag="VBC81_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6912601.1"
                     /translation="APGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKL
                     YNVIDESYLKSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFI
                     MGQSAVGHTDILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQK
                     PVYLRPPYGATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMH
                     DIHQTSIDALPTIMDYLTTQGYYFVTVGELYSTP"
BASE COUNT         2462 a         1435 c         1198 g         2442 t
ORIGIN      
        1 ttataaatgt tctataaatt atcatgagca aaaaaagcgt aaactttcgt ttacgctttt
       61 tattatttgc tagcaataaa atcctttaag gcctggactt tatcaactcg ctcccatggc
      121 aaatcgatat cagtacgtcc catatgccca taggctgctg tttgtcgata aataggtcgt
      181 ttgaggtcca acatattaat aatgcctgct ggtcgcaaat caaatatttg gcgtacagct
      241 gcctctaggt ctgcttctgc tataacacct gttccaaatg tatctacacg cacagagaca
      301 ggctgagcaa caccaattgc ataagcaagt tgtacttcta cttttttagc taaatcagct
      361 gcaacaatat tttttgcaat ataacgagct gcataagatg ctgaacgatc tactttagta
      421 gcatctttac ctgaaaatgc accaccacca tgacgagaat aaccaccata agtatctaca
      481 ataatcttac gacctgttag tcctgaatct ccttgagggc ctccaataac aaagcgccct
      541 gtaggattga taaagaattt tgtttggtca tctaaataat ggcttggaat aacttcattg
      601 ataactttct caatcacgtc cttatggagt tggtcattag ttacattagg atcatgctgt
      661 gtcgaaataa ctactgcatc cacacgaatt ggttgatcat tatcatcata ttcaaccgtt
      721 acttgagatt ttgcatctgg tcgaagatag gtgagttccc cagacttacg tagatctgtc
      781 aatttcttaa ctaactgatg agctaaagaa attggcaatg gcattaactc aggtgtttca
      841 tcaacagcaa acccaaacat caaaccttgg tcacctgcac caattaaatc taaaggatct
      901 tgttcaagag aaccacgtac ctcaagagct tcattgactc cctgtgcaat gtctggcgac
      961 tgttcaacga gtgacggatg cactccgaca gattcagctg agaagccata ttcagcttta
     1021 tcatagccaa tttcagcgat tgtgttacgg acaactcggt taatatcaac ataggctgtc
     1081 gttgatatct cgccaaatac atgaaccgaa cctgtataaa cagctgtttc ggctgctacg
     1141 tgggcatctg gatcttgctc taaaattgca tctaaaatcg catcggaaat ttggtcagca
     1201 atcttatcgg gatgtccctc agatactgac tcagacgtga aaagtttacg ttctgacata
     1261 aaaatgtccc ccctaaaatg taaaatgtgt cgagttacaa agaactagcc tctaggctac
     1321 cttttcggga ctcatatagc tattgtacca ctatttcatc ttttgtgaaa taagttacca
     1381 ataaataaaa aattatctct attttcttta gtcttttttc tcgatttata ctatactaga
     1441 tttatgaaaa cctacgaaaa aatttatcaa atactatcaa aaaataacaa ctttattagc
     1501 ggagaaacta tggcaaatca acttaatatt tcccgcacag ctatttggaa aggtataaaa
     1561 acgttagaag aattaggcct agagattgag tctgtcacta acaagggcta ccgattggta
     1621 tcaggtgaca tattattacc agaacaactc gaacaagaaa ttggtattaa ggttagtctt
     1681 aataataata gtgcttcaac ccaactagat gctaaaatgg ggatagaaag caaattaaag
     1741 actccacacc tttttttagc tcctaatcaa aaaaaagcta aaggacgttt tgatagacct
     1801 ttctttacat caaatcaagg cggcatctat atgtctcttt tattacaacc taatgtaccg
     1861 attgaagata taaaacctta tactgtcatg gtagcctcta gtgctgtaaa agctatctca
     1921 cggttaacag gaattactcc tgaaattaag tgggtcaatg atatttattt agataataaa
     1981 aaaatagctg gtattttaac cgaggctatc gcttctgttg agagtggatt ggtaacgaat
     2041 gttattatag gattaggtat caatttctat atcaaagagt tccctagggc gttaactaag
     2101 cgcgcaggta gtctctttac agagcaaccg accataacta gaaatcaatt gattacagaa
     2161 atctggaatc tctttttcaa tattccacta gaggatcact taaaggtata tagagaaaaa
     2221 tccctagtct tagataaaac cgttagcttt atggatggtc aaacaatgta ttctgggaaa
     2281 gctattgata ttactgataa aggatattta gtggttgagt tagatgatgg tcagttgaaa
     2341 acacttcgca gtggcgaaat cagtctttct tcttggtaga tttgataagc tcttttgtga
     2401 attttgaaat cgcatacacc ttttgaagat ctcgaagtaa taaaactccc caaaaaatag
     2461 caaaaatcca ggagccatcc ataacggatt gattgaaaaa ataagtttca aaagagcata
     2521 aaagcgccat aaaaataaat tgtcgttttg tcatatcacc attataatag aatctactaa
     2581 ataattaaaa aacttatcaa aaaaatagtt gcctgttacg ggcaactatt tttagttacg
     2641 ttttaatctt caacgattgc tattcgttct gctagataag aaaagtcttc tggaatctga
     2701 tgattaactt caggttctgt ttccgtcttt tgagctttca ttttctttgc aaaatcgctc
     2761 cgaatttgat taaaatcatt tctaggtaca gctaaaatat taggtgaaaa acctgctgct
     2821 ttactcataa tattaccaaa tatatcattg agatcggtac gtttcatagc ttgttcagca
     2881 ttaaaggccg catcaaatgc caaaatagca ttctcgctat tagctaagac aggttcagat
     2941 cccattaata aggctctatc ttgagccgta atattatcta atatctcgtt ccaagcactc
     3001 tttaaagctt ctaaatattc acgtgaacgt tggctatcta caaccgtttc ttccataatg
     3061 gtcaaaatct ttgtacggtc aactttgaat gtgaatgtct tattattaac ctttactttt
     3121 tgagttgatg aactttgatc tgctcgagag agttggctca tttcgttttt taacgatttt
     3181 aactctcgac gaagactatc taattctgct gtcacatctg cgggtatgtt tgacgaattt
     3241 ttttctacca tttcagacaa ttgaatggtc atcatctctg cataaacttt aggatggcta
     3301 ccatttttta tctcaggaag aacagatgtc actttatcaa tcatttcaaa taaaatatct
     3361 tgtttaacat caagattggc cataaaagta tcacttgaat gactattttc accacctgct
     3421 tgaacaacaa gtaaatctct taagtacatc aataaatctg ttgcaaaacg actcatactt
     3481 ttgccactat caaagattac ttcaagttta gcgagtgctt cagttgtatc gtgagccaat
     3541 atattcgata cataatcatc tatagcagat aaggaaatgc tccctgtaat ttcttctgct
     3601 acatctaaag aaatgtggtt atctttcgct aaactcaagg cttgatcgag aattgataaa
     3661 gcatcacgca taccaccttc ggctcgtcta gcgactagtg tcagagcatc caaatcatat
     3721 gaaatagctt ctttatctaa aatctgtgct aaatgatctc gaatagctaa taatttaatc
     3781 gctttaaatt caaagcgctg gacacgagat aagattgttg cagggatttt atggagttct
     3841 gttgttgcta atataaaaac aacattttct gttggctctt caagtgtttt caatagtgca
     3901 ttgaaggcac ctgtagacaa catatgaact tcatcaataa tataaacttt ataggttgcc
     3961 ctacttggag cataagttga tttgtccctg atatcacgaa tttcatcgac accattattt
     4021 gacgcagcat caatttcaat gacatcctct aaactaccat ttgtaatatc tcgacaaata
     4081 tcacaatgat tacaaggttc gccattaact tgatttgggc aattcatcgc ttttgcaaat
     4141 attttagcag cacttgtttt tccagttccc ctaggtcctg aaaaaaggta agcatggcta
     4201 atttttttgc tggacacagc ttgctttaaa gtagttgaaa taacactttg tccaaccatt
     4261 tcatcaaatg tttggctacg atatttccga taaagggcct gatacattac ttttcaactc
     4321 caaacatatc caaattccaa atcgtatgtt ctactagaat acctacaaat ttttctaagt
     4381 attcttgatc aatctcatca taatctgcta ctaaagaaga atctaaatct agaactccta
     4441 gaagtttgcc atttttaaac atgggtacta cgatttcact catagctttt gaatcacagg
     4501 agatatagtt agcatgcttt gtaacatcat caacaatcag cgtcttagca gtttgtgcag
     4561 attcaccaca aacacctttt cctaaagtaa tatgcacaca tgatacacca ccctgaaaag
     4621 ggccaagaat taactcctct ccatcaaata aataaaagcc tgtaaataca gaatttggaa
     4681 gcatagcatt tagcatagct gaagcatttg ataagttggc aagagcattt gtttcatctg
     4741 agaatagagc ttgcgcttgt agtaataata attgataatt ttcgattttc ttagacttgt
     4801 tcatagcact tattatagca taatttgaaa gttactaaaa ctaaataacc atcctatata
     4861 aaacaaaaaa ataaacacaa accattgtgt ttattttgct tcatttaaga tgctagccac
     4921 tttggtattg attaaatcaa tagcgacaat attacttact ccttctggaa ttacaatatc
     4981 cgcataacgc tttgttggtt cgataaattg atgatacata ggttttacaa cttctgtata
     5041 ctgttcaata atactatcta aacttctatc gcgctcttcc atatcacgct taatacgacg
     5101 aataatacga atatcatcat ctgtatcaac aaataatttg atatccatta aatcacgcaa
     5161 acgctgatct tctaatacca aaattccctc aacaataatg acatcctgag gttcttggcg
     5221 gatagttcta tcactacggg tatgttttgt gtaatcataa accggaatat cgaccggacg
     5281 cccctcaatc aattcgttca attgctcaat catcaaattg gtatcaaaag caaggggatg
     5341 atcataattg gtttttacac gttcttcaaa agtcaaatga ctttgatctt tatagtaaga
     5401 atcatgttca atcatgataa ttttttgatc tgggaaattt gataaaatcg cccgagaaac
     5461 actggtttta ccaccaccag atccacctgt aacaccaata ataataggtt ttttacgcat
     5521 ttgtcactcc aaattaaatt tatcctctat ctattgtact ataaacttat tttttttaaa
     5581 gtctaaattt tattgaaaac gctctaaaaa taaaaagcat gttataataa caatgacaac
     5641 atataaaata acaagaggat tcttatgata acaaaatttc cagaccaatg gcaggataaa
     5701 ttaacccagc gtcaatttga tgatctaaca gatattcaaa ataaactttt tcaacctatt
     5761 actgatggtg ataatatcct tggaattagc cctacaggta ctggtaaaac tctagcatac
     5821 cttttcccaa cgcttcttaa gttgcaacct aaaaagagcc aacaactctt aattttagct
     5881 ccaaattcag aattagctgg acaaatcttc gacgttacaa aagaatgggc tgaaccatta
     5941 ggactgacag cacaactatt cctttcaggg tctagtcaaa aacgccaaat tgagcgacta
     6001 aaaaaaggac cagaaatcct tataggaact gctggacgcg tatttgaact tgtgaaacta
     6061 aaaaaaataa aaatgatgaa cattaatact atcgtccttg atgagttcga tgaattactc
     6121 ggcgactcac aatatcattt tgtggacaat attattaatc gcgttcctcg tgatcagcaa
     6181 atgatttaca ttagtgccac caacaaactg gacaatagta aacttgccga taacactata
     6241 acaattgacc tatctgatca aaaacttgat actataaaac ataattatat tacagttgat
     6301 aaaagagagc gtactgactt acttcgtaaa ttttcaaata ttcctgattt tagaggtctt
     6361 gtctttttta acagcttatc agacttaggt gcatgtgaag aacgtttaca atttaatagg
     6421 gcctcggcag tttctttagc aagcgatatt aatattaaat tccgtaaagt tatcttagag
     6481 aaatttaaaa accatgatat ttcattactc ctaggaactg atttggtagc tcgtggcatt
     6541 gatatcgaca acctagaata tgtgattaat tttgatatcg cacgcgacaa agaaacatat
     6601 acacatcgtt ctggacgaac tggtcgtatg gggaaagagg gatgtgttat tacatttgta
     6661 acacataaag aagagctcaa acaacttaaa aaatacgcca ccgttgcaga acttgtactt
     6721 cacaatcaaa aattacactt aaaataaaaa agaaacaagt aacttgtttc tttttttatt
     6781 aaggcgttga atataattca cctacagtaa caaaataata accctgggtt gtcagataat
     6841 ccataattgt aggcaaagca tctatggatg tctggtgaat atcatgcatc aaaatcactc
     6901 ccccaggacg taattgtttt ttgatattgg tcatcattgc ttgtgtatta tgatttttcc
     6961 agtcttctgt atcaactgac cacaacattt ctttcaatcc tgtcactgtt tttacagtag
     7021 catttgttgc tccgtaaggg ggacgtaagt acactggttt ttgtcctgtt gctttcatga
     7081 ttagatcatt agtcttatga atctcttcct tgattttctc cgctggtagt ttggttaaat
     7141 taggatgatc ccatgtatga ttaccaatct catgaccttc agcatgcatc ctttgtaaaa
     7201 tatctgtgtg ccctacagca ctttgtccca ttataaagaa agtcgcttta gcattatatc
     7261 ttttaaggat ttctaaggct ttaggagtcg ttttttcatt tggtccatca tcaaaggtaa
     7321 gtgcaacacg tggtgcttta gcttttaagt tagaaactaa atctagatca cttgatttta
     7381 aatatgactc atcgatcaca ttatataatt tatcaagtgg taattttaaa tcattaataa
     7441 ttaacttacc atctttaaca tctagcccat ctgttactct tccagttttt aaaaattctt
     7501 tattgctatc atgttccttt agttgagttc ctggagc
//
LOCUS       DATRNX010000089         7413 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRNX010000089 DATRNX010000000
VERSION     DATRNX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..546
                     /locus_tag="VBC81_001571"
     CDS             <1..546
                     /locus_tag="VBC81_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6912602.1"
                     /translation="MGGNNISYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDIS
                     GHKTQVVQGTTTAKQLENFNKEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPT
                     VNAIIKNQGLTNLKTIPLTMRDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIA
                     KEYLGGDYVPNEKDLVTPKEK"
     gene            687..1511
                     /locus_tag="VBC81_001572"
     CDS             687..1511
                     /locus_tag="VBC81_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6912603.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            1645..3021
                     /locus_tag="VBC81_001573"
     CDS             1645..3021
                     /locus_tag="VBC81_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6912604.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            3014..4084
                     /locus_tag="VBC81_001574"
     CDS             3014..4084
                     /locus_tag="VBC81_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6912605.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            4081..4773
                     /locus_tag="VBC81_001575"
     CDS             4081..4773
                     /locus_tag="VBC81_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912606.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(4822..5877)
                     /locus_tag="VBC81_001576"
     CDS             complement(4822..5877)
                     /locus_tag="VBC81_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6912607.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(5991..7358)
                     /gene="brnQ"
                     /locus_tag="VBC81_001577"
     CDS             complement(5991..7358)
                     /gene="brnQ"
                     /locus_tag="VBC81_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6912608.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
BASE COUNT         2530 a         1211 c         1421 g         2251 t
ORIGIN      
        1 atgggtggaa ataatatttc ttattcatca gagagatctc aaaaatattt attttcatac
       61 ccaataggct ctactccttc agttttagca gttcctaaga atagtaatat caaagcttat
      121 aatgatatta gtggtcataa aacacaggtt gtccaaggaa cgacaactgc caagcaatta
      181 gaaaatttca ataaagagca tcagaaaaat cctgttactc taaaatatac taatgaaaat
      241 attacacaga ttctaacgaa tttgagtgat ggaaaagctg attttaaact ttttgacgga
      301 ccaactgtta acgctatcat aaagaatcaa ggtctcacga atttaaaaac catccctttg
      361 actatgagag atcagcctta tatttatttc atctttggtc aggatcaaaa agacttgcaa
      421 aagtatgtga ataatcgcct taaacaatta cgtaaagatg ggacactttc aaaaatagct
      481 aaagaatacc ttggaggtga ttacgtacca aatgagaaag atctcgttac tcctaaggag
      541 aagtagtaat aaaaactaag gagatgtcaa tccttagttt tttgttatat agtttaaaac
      601 tataacaaga gacagtttat aacaatttga ttctaataac ttcctatggg ataatatctt
      661 tatcacaaaa attgagggaa tttattatga agttaaaaca tattgtctta ggattagcct
      721 taacaacact tttaggagtc acatttagta atcaagaagt ttcagcaagc tcaacttcaa
      781 gtaaagttgt taaagttggt gttatgacct tttctgacac tgaaaaagca cgttgggata
      841 aaattgaaaa gctagtaggt gataaagcta aaatcaaatt tacagaattt acagattata
      901 cacaaccaaa tcaagcgaca gccaataagg atgtggatat taatgccttt caacattaca
      961 atttcttaga aaactggaat aaggaaaata agaaaaactt aattccactt gaaaagactt
     1021 acttagctcc aattcgtatc tattctgaga aggtaaaatc tcttaaaaaa ttgaaaaaag
     1081 gagccactat tgcaattcca aatgatgcaa caaatggtag ccgtgcattg tatgtccttc
     1141 agtcagcagg tttaatcaaa ttgaatgttt ctggtaagaa ggttgcaaca gttgctaata
     1201 tcacatctaa taaaaaggat attaatattc aggagttaga tgcgagtcaa acaccacgtg
     1261 cactcaaaga tgtagatgca gctattatta ataatacata cattgagcaa gctaatttaa
     1321 aaccttcaga tgctatcttt gttgagaaat cagataaaaa ttcaaaacaa tggattaata
     1381 tcattgcggg acgtaaaaat tggaaaaagc aaaagaacgc taaagctatc caagctatct
     1441 tggatgctta tcacacagat gaagtgaaaa aagttatcaa agatacttca gctgatattc
     1501 cacaatggta acgtgaattg actgtataag tttaacaggg aatcctaata ggatacaact
     1561 tatcagccat atagccataa agggcctggg acgaatacaa tgtgccaggc tcttggattg
     1621 tttttgaagg aaggtggatg aaagatgact ttaacaaacg aagaaaatca aattcaaaag
     1681 tttctatctg atactattgt tcaggatgat atcaatattt taagagattt aattgcaatt
     1741 aagtcaattt ttgctcagaa agttggttta aatgacttat caagctatct tggagaagtt
     1801 ttcagaaaag ctggcgcaga agtcattata gatgacagct atagcgctcc ctttatcgtg
     1861 gcaaatttca aatcatcaaa agtagatgct aagcgtatta ttttttacaa tcattatgat
     1921 actgtaccag cagatgaggt agaacagtgg actgaggacc cttttacact ttcacttcga
     1981 tatggtaaga tgtatggacg tggtgtagat gatgataaag gtcacattac cgcacgttta
     2041 tcagcagtta aaaaatattt atcaagacac aaaggggaac ttcctctaga tatcactttt
     2101 attgtggaag gagcagagga atcagcttca gtaggccttg attattattt agaaaagtat
     2161 caagagcagc tacaaggggc tgatttgatc gtgtgggaag atggccctaa aaatcctaaa
     2221 ggacagttgg aaattgctgg tggaaataaa ggcattgtca cttttgattt atcagtaagt
     2281 tcagcggatg ttgatattca ttcatccttt ggtggggttg ttgattcctc gacatggtat
     2341 ttaattcaag ccctcaatac gttaagggat aacaaaggtc atatattggt agaaggtatt
     2401 tatgataaag ttattccccc tactaagaga gagttagaat tagttgaaaa atattcttat
     2461 agatctgcaa aggcgctaga aggtgcttac caattagttt tgcccagttt agcagatagt
     2521 cataaaactt ttttgagaaa gctttatttt gaaccttcta tagccattga aggtataacc
     2581 tcaggttatc aaggtgaagg cgtcaaaaca attttacctg cttatgctaa atgtaaagca
     2641 gaggttaggt tagtaccagg gttaaccccg aaaggtgtac tagattcaat acaaaatcat
     2701 ttgaaagaga atggctttaa agacattgag ctaacctata cccttggtga aatgtcttat
     2761 cgtagtgata tgtcagcgcc atctatccta aaggttgttg atttagctga gcaattttat
     2821 ccagaaggga tatctctgtt acctacctca ccaggtacag gaccaatgta tttagttcat
     2881 caagctctaa gagcgccaat tgcagctatt ggcataggac atgccaatag tcgagaccat
     2941 ggcgttgatg aaaatgttag tattgctgat tattacaccc atattgaatt agtggaggct
     3001 ttaatagaat catatgagta aagaaccaat tattaaatta gacaacattg atgttacttt
     3061 ccaccaaaag aaacgtgaga ttaatgctgt taaagatgtt actatccata ttaatcaagg
     3121 tgatatatat ggaattgtcg gttattcagg agccggaaaa tctacccttg ttcgtgttat
     3181 taatttgcta caagaacctt cagcaggtaa aattactatt gatgaccaag ttatttacga
     3241 caacaaggtg acactgacta gcacacaatt acgcgaacag cgccgtgaaa ttggaatgat
     3301 ttttcaacat tttaacctaa tgtcacagtt gacagcagaa cagaatgtag cttttgctct
     3361 taaacattca ggtttatcaa aagaggcgaa agcagctaag gttgctaaat tattagaact
     3421 tgttggatta tccgatcgtg ctcaaaacta tccttcacaa ttatcaggag ggcaaaaaca
     3481 gcgtgttgcc attgcgcgtg cattggctaa cgatcctaaa atcttaattt cagatgagtc
     3541 tacctctgca cttgacccta aaacaacaaa acaaatactt gcccttctcc aagatctgaa
     3601 taaaaaattg ggacttacga ttgttttgat tacacatgag atgcaaattg tcaaagacat
     3661 tgctaatcgc gttgctgtta tgcaaaatgg taaattgatt gaagaaggat cagttttaga
     3721 cattttttca catcctagag agagtcttac acaagatttt ataaaaatag ctactgggat
     3781 tgatgaggca atgcttaaga ttgagcaaca agaagttgtc aaaaatctac cagtaggtag
     3841 caaattagtt caactgaaat acgctgggca tagcacggat gagccgcttt taaatcaaat
     3901 ttataaagaa tttgaagtaa ctgctaacat cctctatggg aatattgaaa ttttagatgg
     3961 tattccagtt ggagaaatgg tagttatctt atcaggtgac gaagaaaaat taagacaagc
     4021 ctgccaagca attacagatt cgcaagttca attaacgtta ttgaaagaag gtggtaaagc
     4081 atgatagagt ggattcaaac acatttacca aatgtatatc aaatgggttg ggaaggtgct
     4141 tacggctggc agacagctat tgtacaaacc ctttatatga ctttttggtc gttccttatt
     4201 ggaggtttaa tgggattgtt aggaggttta ttccttgttt taactagtcc tagaggagtt
     4261 attgctaata aattagtatt tggagtttta gataaagttg tttctgtttt tagagctctg
     4321 cccttcatta ttcttcttgc tttgattgcg ccagtaactc gcgtaattgt aggaacaaca
     4381 cttggttcac cagcagcttt ggtacctctt tctttggcag ttttcccatt ttttgctcgt
     4441 caagttcaag ttgttttagc tgaacttgat ggtggagtta ttgaggctgc acaagcctca
     4501 ggtggaacac tttgggatat tattgtagtt tatcttcgtg aaggtctacc agatttaatt
     4561 cgagtatcaa cggttacttt gatttcttta gtaggtgaaa cagctatggc tggcgctatt
     4621 ggtgcaggag gattgggttc tgttgctatt actaaaggat ataactattc tcgtgatgat
     4681 attactttag tagcgactat tctgatttta ttattaattt tctttatcca atttttaggt
     4741 gattttttaa cacgtcgctt gagtcataaa taaaaatgtg agaaggctat tcttctcaca
     4801 ttttcatttg tttaaaaatt atcaggctgg agtaatatcg ttttcgatga taactttgag
     4861 agtattgttt tcttcagcag ctttaaatac cttataagct tcttcgattt cagatagctt
     4921 gaagtgatgt gttactagtt gtgttgcatc gattttgcct gtctcaagaa cttcaaggag
     4981 catttcagtt gtattagcat taactaatcc agtattaagg gttatatttt ttatccataa
     5041 atcttggagg ttaaattcaa caggtttacc atgaacacca acatttgcga tatgtcctcc
     5101 aatagaaact acattttgac aaatatcaaa tgttgcagga taaccaacac attctaatac
     5161 gacatcaacg ccacggcctt tggtaatttt atcaatttct tctttaactt cttgagtaga
     5221 cgttgataga atggtgtgtg ttgcaccaaa ttttttagag gcttcaagtc gtttttgaga
     5281 taaatcaacc ataataattt tggcaggaga atagaattgt gcagttagta aggcagatag
     5341 tccaattggt ccagcgccta caatacaaac agtatcacct ggtttaatgt gagaaggtaa
     5401 gacgccgatt tcgtatgaag ttggtaaaat atcagataac ataaccaatg catcgtcaca
     5461 aacaccttca ggtgcatgat aaaggctacc atctgcatgt ggaatatgaa catattctgc
     5521 ttgagtacct tcaataagat gtcctaaaat ccacccgcca tcttcacaat gtgaaggcaa
     5581 ctggtgctta cagtaatagc aagtattaca ggcagtgaca caagaaataa ttactttatc
     5641 tccgacttta aaatgattga cagcagaacc aacttcttcg acgataccaa tgccttcatg
     5701 acctaaagtt gttcctggtt tacaagcagg aacatcgcca ccaaggatat gtagatcagt
     5761 accacaaatg gttgttttga caagtcttac aaccgcatct gtgggtttta ttacttgagg
     5821 tttagcctta tcagtaagtg ataattcatt agctgaaaga taagttgcag ctctcatatt
     5881 agagttcctc caaaaattat ttattttcaa ggctattata ccaccaaaag aaagcggtta
     5941 caataattat tttaaaataa aagcagtaat tttcgtactg ctttaaatta ttatgctttt
     6001 acttttttta gaataagacc aactaagaca ccacaaattg caaaactaat ccatcccata
     6061 gaatattcac ctaaaggaac aactttagta aagaatgtgg tcaaagcgga tggaatggta
     6121 aataaaccag tcatttcgcc gagttttgat aaggcatcgt ataaagcagc gacagcagtg
     6181 gcggcaatag aagtttggta gacaatgcgg ctactttcaa atttttgatc aaaaaagact
     6241 aagaaaatta agactatagt taatggatag agaaggtaga gaactggtag tgaccaacgg
     6301 ataatctctg ataaaccacc aaaatagaac gtaatagcaa ttaaagtaaa gattgtagcc
     6361 caagtaatgt gactaatctt tggaagtaat ttgtggaagt attccgcaca agcagtgatt
     6421 aatccagtag ctgttgttaa gcaagcaaga aaaatagccg tacctaaaat agcttgtccc
     6481 acaattccta aatagaagtt agctgattga ctaagaacat gcccaccgtc tattggagta
     6541 ttgtgaagta agaaggaacc atttgcaaat ttaaacaagc tttgtgaagt tgcaccaata
     6601 cgtcctacaa aaatataaat aaaagctaaa agaaatgtag cgatagcacc agatttaagt
     6661 gtcatagaag taatttcttt tttggtaatt gctccgtact gtttacttgc ttgaataacg
     6721 ataatagcaa aagcaattga tgcaagggca tccattgtac catacccctg aatcagtcct
     6781 gctaggaatg gtaaatcctt gaaagcatcg ctgacattcg ttgaagcgtt atgtggtgtt
     6841 ccaacgatag cagctggatg gatgagtgag gcaataatta aaatactaat aacagttagt
     6901 aaaaagggag ttagatattt accaatacga tctacaatct gactaggtct aatagctaag
     6961 aaataagaaa taccaaagaa aattaaacca tagataattt tgactaagga gctgtcgcct
     7021 aagataggtt gaataccaac tgagaatgag gttgcccctg ttcgaggaat ggcaaagaaa
     7081 ggtccaatag ataggtatag tgctactgca aagaaaatag catatgtttt agagacaggt
     7141 ttggtgaagc tttcaacatc ggttgctcct gagtacgtca cagctgcaac accaagtagg
     7201 ggtaaagtta cgcctgttaa gcagaaccca atgatagccc acgcccaatt agaaccagag
     7261 tagataccta agtaggctgg ataaataaga ttaccggctc caaagaacag cgcaaaaagc
     7321 atcaagccga taatgatata agtgtttttt agtttcatca atgtaccttt ctgtacaaaa
     7381 aaatatttta ttttcgaaat tgattgaata ttt
//
LOCUS       DATRNX010000090         7379 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRNX010000090 DATRNX010000000
VERSION     DATRNX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            279..802
                     /locus_tag="VBC81_001578"
                     /pseudo
     CDS             279..802
                     /locus_tag="VBC81_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            873..1978
                     /locus_tag="VBC81_001579"
     CDS             join(873..1149,1149..1978)
                     /locus_tag="VBC81_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6912609.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            2202..3334
                     /locus_tag="VBC81_001580"
                     /pseudo
     CDS             2202..3334
                     /locus_tag="VBC81_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3374..3613
                     /locus_tag="VBC81_001581"
                     /pseudo
     CDS             3374..3613
                     /locus_tag="VBC81_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3812..6280)
                     /locus_tag="VBC81_001582"
     CDS             complement(3812..6280)
                     /locus_tag="VBC81_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6912610.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(6293..7213)
                     /gene="lmb"
                     /locus_tag="VBC81_001583"
     CDS             complement(6293..7213)
                     /gene="lmb"
                     /locus_tag="VBC81_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6912611.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         2282 a         1452 c         1336 g         2309 t
ORIGIN      
        1 tgagcggtta aataactctt tatgaggaat cattgcaaga actgatttat cattctttag
       61 tgatcttatt aattcttttg aagagttggt attatgcttt atcatattac ctaaaaagaa
      121 taattttgct gtgacataac ttgctcgagt tttaaagtca aatgcctctg ttttaaattc
      181 ttcaagtctt tcagaaagat ggatgtatac tataaaagta gacaaaactt tgtgtgttac
      241 aatctgtttt aaaagacaca aaaacgaaag gacaacctat atcaacattt aaacgctatg
      301 atgaagaatt caaacaatct cttgttaacc tttatcaaac tggaaaaact cagtctgaac
      361 tctgtaaaga ctatggggta tccacttcta cgcttgcaaa atggatcaaa cagtattccc
      421 aagtcaaact cgaagataat tctgtactta ctgccaagca aatacaagag ttacaaaaac
      481 gtaatgcgca gcttgaagag gagaacctta tcctaaaaaa agcaagtgcc atattcatgc
      541 aaaacttaaa gtaagactcc tcgctgtcta tcggttacgc tttgaacacg ccacaacaac
      601 cttgtgtcgt gttttacgtg tcaatcgctc cacttactat aaatttctaa aacataagcc
      661 ctcaaaaaga gaattggata atcaaattta tagaaaacaa atacttgaga tttataccaa
      721 agcgaacaaa agacttggtg tgaagtctat caaggtcatt cttcaaagag actatgacac
      781 aaaaatctct gaaggaagaa tttgaatagg ttacactaaa ctagacagaa attataaagt
      841 gttctacact taagaaaaac tggagaaaaa ccatgtctag aaaagtacgt cgtcacttca
      901 ccgatgattt taagcaacaa attgttgacc tttacaatgt tggtagaaag cgtagcagtc
      961 tcatcaaggt atatgagcta accccttcca ccttcgataa gtgggttaga caagccaaaa
     1021 caactggttc attcaagtct gttgataatc tgacagatga acagcgggag ctaattgaac
     1081 tcagaaaaca caataaagaa ctcgaaatgc aattagatat cctaaagcaa gcagcggtga
     1141 ttatggcaca aaaagggaaa taatcactgc taataaggat aaatacagca tttcaaagat
     1201 gtgtcgctgg ctgaatatgc cacactcaag ttattactat caagccgtgg agtcagtatc
     1261 tgaaacggag tttgaagaaa ctattaaaag aattttcctc gatagcgagt ccagatacgg
     1321 atccagaaaa atcaaaatat gcttgaataa cgaaggtatc acactttcac gtcgtcggat
     1381 tcgacgcatt atgaagcgac tcaatttggt ttctgtttat cagaaagcca ccttcaaacc
     1441 acattctaga ggcaagaatg aagcccctat tcccaaccac ttagacaggc aatttaagca
     1501 agaaagacca ctacaggcct tagtcactga cttaacctat gttcgtgtag gcaatcgttg
     1561 ggcttatgtt tgcctcatca ttgacctata caaccgtgaa atcatcggcc tgtctcttgg
     1621 ttggcacaag accgctgaac tcgttaagca agccatacaa agcatccctt acgccctgac
     1681 caaagtcaag atgttccatt cagatcgtgg caaagagttt gataatcagt taattgatga
     1741 aatattggaa gcctttggaa tcacacgttc gcttagtcag gctggttgtc cttatgacaa
     1801 tgccgtagct gaaagtacgt atcgtgcttt caaaattgaa tttgtttatc aagaaacctt
     1861 tcaattgctg gaagaactag ctcttaagac taaagactat gttcattggt ggaattacca
     1921 ccgcattcat ggtagtctca actaccaaac accaatgact aaaagattaa ttgcctagaa
     1981 aaacacttta taaatgttgt acagaaaagt gttgcctttt cacttaataa aagaaagatt
     2041 gaaggaacac ttctctttca ttccgaccaa gggtcacaat ttaagtccgg gaaatttaga
     2101 aaaataattg atgacaacaa taggcactga atccctaaaa tgagacaaaa gaaaaacact
     2161 cctgttgaaa tttagtaaac tagaaacagg agtgttttat tatggttaaa aaagcatatt
     2221 catgggaaac taagctggct tgcatcgata tgaagaaagc aggcaagtct aacagagtta
     2281 ttatggagac tttaggcatc aaaaataata gtcaaatcta tacttggatg aaatggtacg
     2341 aaaacgagga gctttatcgt tttcatcaag gtgttggcaa gcaatatacc tacggtaaag
     2401 gcttggaaca tctgtctgaa gtggaacaat tacaattaca ggttgacctc ttaaaaaagt
     2461 atcgaggctt gataaggaaa tcgataaagt aagtcttatc aagcttgtgg aagactataa
     2521 aaacaactac ccgatagttt tcattctaga ctgttttaag gtcaagcggt cgacctatta
     2581 ccgttggaaa aaggagtatg agaagccgca aaaaaagatg accttattga attaattgaa
     2641 cagctctgta tggagaatca ctttatttat ggctatcgta ccattacacg attgctcaaa
     2701 aaaatccacg gattgactgt taatacaaag aaagtctatc gtatcatgaa aaataatggc
     2761 tggctttgtc ggacacgtac caagaaagtt cccaatttgg gtaaagctta ttatttaaca
     2821 gataataaat tgagtcggga tttccatgcc gataagccta aggaaaagct cgtaacagat
     2881 attacctacc tatatttcgg taactgtaaa ttgtatctct catcaattat ggatctctat
     2941 aaccgagaaa ttatagctta cactatctca gattgtcagg acacggattt tgtactagac
     3001 acccttaatc agttaaaatt gcctaaataa aggagcaatc ttacacagtg accaaggctc
     3061 agtgtatact tctatggctt actaccaggc ttgcacggaa aaagacatta tccgctcaat
     3121 gtctcgaaaa ggaacacctg cagataatgc ctgtattgaa tggtttcaca ccgtcctaaa
     3181 gactgaaacc ttttatttcc ataataggag aaaatacaac aaagatagta taacaaatat
     3241 tgttaaaaat tacattacat tttataatga aactagaatt caacagagat taaacgacca
     3301 gtcgcctgta cagtacagaa aactgattgc ctaaaagtgt ttttcttgcg tctcactatt
     3361 agggtgcagt gccaatatca tgcattcttt ttctaatcct ggatatcctt atgataatgc
     3421 cgtaactgaa gcatttttca agtatttaaa gcatagacaa atcaaccgaa aacattatca
     3481 aaatatcaaa caggttcaat tagactgctt tgaatacatt gagaattttt ataacaatta
     3541 caacccacat acggctaatc taggactaac ccctaatcag aaagaagaaa attattttaa
     3601 ctcaataaaa taacacagtt ttctgtctac ttatttgaca ttagtccaag aatcctcctc
     3661 tttttttact attctaacat ggataccatt tctttgcaaa agaaaaccgc tattatccaa
     3721 cttttatcac tactcgttac atagaatgac ttcaaaatca ccgtaacttg ctttgacaaa
     3781 gcagtgcttt aacaatgaga tcttcttttg gttaagggtt tatttgttga agtgtcttga
     3841 tatcataagt taccaattca ccatttttat tataaaattg gacaccttct ggttggaaaa
     3901 tgagatactt aggatcgata ttagcttttt gtgctaattg attgatatga tcttctaggg
     3961 ttgctctatc tagaccatag tctggtaaac tgtctataaa gtcatctgat tctttttctt
     4021 ctttactggc ttcacttggc tgttggtttt cattgctctt atctgcctgt tgcttttctt
     4081 caggtttatc cgtatcagta gcatcaccag catttttctt tgccaataac tcttgagctt
     4141 gttgccactc agcttgggat agatcggatt tattaatggt tctcaatgaa ctgccatctg
     4201 ctcttgggat gctaaaggag ccatctttcc atacaaaagt ttcttttgcc aaaacatctc
     4261 gtggatcgaa aatatagccg tctggtgctg caaaacgacc ttctgctagg gctttttgaa
     4321 cttcttcagc agaatggata atttgccagt ttggcatacc agcacgttta tcaagaggcg
     4381 taacatttgg aatgaccgaa cctgactctt catgccctgg cttagaccat acatccggac
     4441 gaacttcggg gtgttgcatc acatacttga ttgttgcaat ctgattgcgc gtcaaccatg
     4501 aatacggaac gacatggata tgatcaatat gtgggataac aaacgaactt ccagtatcgt
     4561 aagtagcatt accagcatgc atcggcagac ttgacacatc tacagcaagt cgtggctcaa
     4621 tgcctgtatc aacaatgtca taacggtaat gcttcttatc tttaagcatt agttcttgtt
     4681 cggcaaaggc aatctgagtc aaatcaagtt gataacgagc atagaaatag tccttgccat
     4741 cttttggcat aatatagccc actttaccat cttttgttac tttgcgactc acttttttag
     4801 tgtcaaagag tggtttttct ttgccttgtt cctgatccaa agcagcaaca agctcatcag
     4861 cttgaccttt tgctttcacc cagttagcga cctcatccaa ctcatattgt tcaagttctc
     4921 caaatcctat atagtggaaa tgatctccgt gtttagctgt aactcctgat ttatccactg
     4981 aatgaatgga ttctttacta aaaacataag catcactcgt atcatatggt ttaccatcta
     5041 agccttttcc gtaagctttg atgcgatgac caagaaaggt atgtgtcact tctttatctg
     5101 atggttttga gtgatctgaa cctgagtcgt tgtcatcagt ttggccggct aagtatcgat
     5161 ctgctaattc catttcaaga ggtgataact gacttcttgg gataatatga taatgatctc
     5221 catgaggcac cacataccca aaagcgtttg atttgatcac ttgagtcggt tcaaaaatca
     5281 acccatcttc ttccacatga cggtatttca aatcaagacg gtgtagttga tctaaaagtt
     5341 ccttaaaggt ttttccttta aactcatctc tttggtgttt gttttgtgac gcatcatttg
     5401 gcctaggagg cgctggatga taaccaccgt tatctggctg atgaccttgt ccagggttag
     5461 gcgtcacatc aggaattggg gctttcctac gacctggggc tggtgtcggg cggtaatcag
     5521 acggtctagc acctcgacct tgtttttgac tccagtaggc ttgtgcagca gctagctcac
     5581 ttggagacaa atctttttta ggaatataat gatagtgatt accatgaggt actaaataag
     5641 catctcctaa atcatcaatg atatctgtcg gactaaaaat atagccatcg tctgtagtat
     5701 agcgtccttg tctttttgct tcattgactg ccgcaacttc ttctttactg agatgggcca
     5761 cttgagctaa acctttttct ttagcttctt tagttccttt ggctacttgc tcagcaattt
     5821 gttgtttggt tcgaatgttt ttgcgcttac tacctggctt gaggtaaaca taatagttgc
     5881 cattgacttt aataacgtaa ccgtctaaga tttcattgat aacgtctgat tgtttaaaat
     5941 ggtaattagg atccgtcatc aacaactctt cactaataat cgcatcataa ggaactttcc
     6001 cattgtaaaa atgataatgg tcaccgtgtg aggtaacata accttggtca gtaattttga
     6061 ctacgatctg ttcagcagag atgccttctt cagcactgat ttgatccatc gttttgtttg
     6121 ttttaggggc ttttacctta cctttgctat catcaatata ggcaatctga ttgtcctttg
     6181 ttgctagacc catatgatgc ttaccaagct ggtaacttcc aatatgagta gctagtaaaa
     6241 tagcagcaac tgagccgata taaccatatg ttttcttcac taataatctc ctttacttca
     6301 actgttgata gagcacttcc aaatttgctc taagattttc tagatatgtc ttgtttccgc
     6361 ttggagcagc ttcaagtgga cttaatgtct ttactttagc tcctgttgat ttcgcaatag
     6421 catgagcaat tttggggttg acgttgtctt ctgcaaaaat agtcttgacg ttgtattctt
     6481 taacaaagtc ttgaatttct ttcaattggc gaggagaggg ctcttgctct ggagaaatac
     6541 ccgagatacc aagttgtttc aagccgaatc gtttagccag ataagaaaat gccgtgtgtt
     6601 gcgtcacaaa tgtttttgag cgcacctttt taaatttttg agtgtattct tcagttagtt
     6661 gctctgcttc ttttttgaaa gccttagcct ttttagtgta actgtctttg tgtttaggat
     6721 ccaaatgtcc tagctcttta gcgatattaa cagcttcctc accagctaaa acgggatccg
     6781 tccaggtatg tgggtcataa agtgtcgcag ggtcaatgcc ttgtgtgact tccatatctt
     6841 ctagcccttt gactctatct agtgtcagag gttttgacgc ttcaaacaca ttaacctttg
     6901 atttttttaa attagggtct agatcccttg cccaagcttc taaggtatgt gaatggtaaa
     6961 caaacaaatc cgcgtcataa atagctgcca catcatttac agacggttca aaggaatgaa
     7021 tgcctgcacc tgattggatc atcctcacat catttagatc tccagatact tcttttgtca
     7081 tcgcatacat tgggtaaaag ctggttacaa ctgacatgcc ttgcgtaggc tgtttggggt
     7141 ttgctgactt atcacaccct gctatcatta ctaaactcac aaccatagcc atgagaaaaa
     7201 aaactttttt catagtacct cctcaattat aatttaacca gttattaacc agttaatttt
     7261 actcctttat caatgctttg tcaatgacaa ctaccatctt ttatcacatc aactgaaggg
     7321 ataagcaaaa ggcaattgtt gacagctatt tgtcctaact tctttttgat gaggtagca
//
LOCUS       DATRNX010000091         7017 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRNX010000091 DATRNX010000000
VERSION     DATRNX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            60..401
                     /locus_tag="VBC81_001584"
     CDS             60..401
                     /locus_tag="VBC81_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912612.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            435..950
                     /locus_tag="VBC81_001585"
     CDS             435..950
                     /locus_tag="VBC81_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912613.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            1002..3659
                     /locus_tag="VBC81_001586"
     CDS             1002..3659
                     /locus_tag="VBC81_001586"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6912614.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            3705..7017
                     /locus_tag="VBC81_001587"
                     /pseudo
     CDS             3705..7017
                     /locus_tag="VBC81_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2551 a         1148 c         1336 g         1982 t
ORIGIN      
        1 ggagtgctaa aatttcgtta gctggttaac aaaccggtta agtaatgtga ggttctttta
       61 tgagtgaatt cctttcagcc aaacctattg gtcataatga ttcatctaat actattttaa
      121 gaattataga gaatacgaaa tatattcatc actatgatgg aaaagttgtt gtcgaaattc
      181 ctcctcaaaa agagcctatc aaagtctttt ttatggattc acgtcttcat tatttagaat
      241 tttcagacat atgtcggtat gtaactgttg ttttgaaaaa tagtaccgtt gataatataa
      301 ccaatttagg gctagataat catattgttt atgacaatgg tgatgatatg gttgatccgt
      361 tgccgagtat tcagcatgga ttatttctta atgatgatta aagatgtaga caaaaattta
      421 gaaacgagga agccatgaca ttttataaaa agacggacaa atttggattt aggaaaagta
      481 aagtgtgccg cagcctatgc ggtgctttat tgggtacagt agcagttgtt agtctagcta
      541 cagcaagtac agagattcac gctgatgaag caacaacctc acctaccact gtaacgaaag
      601 tacctcagcc agttcaagca gacacaacag ctctaaatac ctctaagact cacagtacac
      661 aagcaactac aactcctgtt gaagctaagg agaataaggt tgtcaaaagt gaaactgttc
      721 aatctgagag ccgagtaatg cctagagata aggtagtaga aaggccagaa acagtaaaag
      781 ctagtgttaa ctcagacgtt agccaaccta ttacaaccac acccccaaca attaatgaaa
      841 aaacagttga gattcctaac ttagctcaag atactaagaa ggtagctcca aaagtaactg
      901 ttacaccaga agttgttaat cagccaaagc agttgcctaa atacgagtaa gaaaactcag
      961 agaaaggcaa taagagtaca gaagcaacta aggcagttac tgtgactaaa ccacaggggg
     1021 cggttgctga gaaagctact ccggctgtgc caaagcctca aaaagtaaga gtaattgttg
     1081 aatttaataa ggaatctatc ttagattatg cgacagaaca gaaaaaaaca gttgcacagt
     1141 taaatcaagc tgatgttgag aaaaaattac aaagcatcaa gcaagagcaa gataaagttc
     1201 ttaagaatat tgaaaaaagt gttcactttg attcaagcaa agtgaagcgt tacgatgcta
     1261 ttattaatgg tgtcgcactt gatattcaag ctcaagaaat tgaaaaatta aaaacgattg
     1321 ctgatgttag aagggtctat gtctcacaag aatatgttca gacaaaacct ttactatctt
     1381 cctcagggca acttattggt ttgccagaag tttggaacaa ctcccagtat aaaggtgagg
     1441 gaactgttgt tgcagttatt gattcagggg ttgatttcaa acaccaagca ctaaaaatca
     1501 aagaaccaaa tcgtgctaag tataataaaa ctagtattga aaaactgatt catgaaaaga
     1561 atctcaaagg aaaattctat tccgaaaaag taccttatgg ttataactat tatgactata
     1621 atgataacct caaggatagc tacggtgtaa tgcatggtat gcatgtaaca ggtatcgtag
     1681 gagctaatga tgataatcaa aaattgtatg gtgttgcacc aaatgctcaa attttagcga
     1741 tgaaggtatt ttcagatgac caacaaaatc ccacaacctt cacagatgtg tggctaaaag
     1801 ctctagacga tgctatttta ctaaaagctg atgttgttaa tatgagtctt ggaacacctg
     1861 ctggatttgt acatgagggg aaagactatc ctgaacttga agttattgca agagcgtgta
     1921 aggcaggaat cgtcattgca gtagcagcag gtaatgaagg aaatatcact gacggtaata
     1981 cttatggtgt taagccgcta gcagaaaatt atgatacagc cttgattgcc aacccagccc
     2041 tcgatgataa taccttggct gttgcctcta tggagaacct taaaaagcat gctcatgttt
     2101 tgaaatttaa agataaaaaa tctggaacag aagttacaga agtcattaat cttcatgttg
     2161 ccccaaacgc ttcaaaaact attattggat tggctgtaga tttgggagca gaagctcctt
     2221 cggaattgtc aaaacatttt gatttaagtg gtaaaatcgc catgcttgaa attccagaag
     2281 ataataaatc taatggattt ttagaaaaag tacaggcgat tactaagtta aatccagctg
     2341 ctatcttgct ttacaataat gcgaaggtta aagatgattt aggcagtcaa ttattagttg
     2401 aatcagaagc agctaaattt aatattgctc gcattaccag aagcacgtat aacaatatca
     2461 aaaataatag caataaaatt attactattt taacagagcg tcaagctatt gataatagtt
     2521 tagcaggaca attatcttct tattcttctt ggggacctac accagactta cgtctaaaac
     2581 ctgagattac agctccaggt ggtcatattt tctcaactgt ggaggataat caatatgctg
     2641 ataaatcagg aacatcaatg gctgcacctc aggtagctgg ggcagctgca gttttaaaac
     2701 aatacattac agataaaaaa attcctgttg ataatgctgc tgattttatt aaattactct
     2761 tgatgaacac tgcacagcca attatcaata aacaatcaaa agatggcaaa acaccttact
     2821 ttgtgcgcca acaaggtagc ggagcaatga atcttgccaa agctcttgtt acgacagtcg
     2881 ttgcaactgt aacagggact aacgataata atgcagatgg taagttggag ttacgtgaat
     2941 taaaagagaa aaaatttaaa gcacgcattc tacttcgtaa ctttggaaaa actaataaaa
     3001 cgtatatcat ctcatcagaa gcgattgcgg acccagttga tgaaaaagga tttcgtacgc
     3061 aaaattcaga acacctagta tcaaaaaaag ctgatgcagt tacaaggaaa gttactgtag
     3121 aagcaggtaa gacattagct gttgaccttg atgtggatta ttctgatgca gaggctttaa
     3181 ctcgcaataa tttcttggaa ggttatctta acttaaaaga tactgaaggt gtagccgatt
     3241 tacacttacc atttttaggt ttttacggtt catggacaga gcaaaaagct attgatgctt
     3301 ttgaaggcat ttctgaaatt ggtaacggtg acaaaaaacg tcgcgttcaa ttctatgtta
     3361 ataaagaaac caacaagacc tcatcaacct ttacaactaa cggcatgttg agtcttccaa
     3421 tctataacaa tacagtattt ttctctccta atagtccttt ctatgacaaa gcaggagttc
     3481 gtattgcagc tttgcgtaat atggagtatg ttcaatattc tattatagat ccagatacca
     3541 ataaagaagt tcgtgtactt ggtagatcac acgacgttag aaaactttat cgcctagatt
     3601 atcgtaatag ttttgccatg atgccagatt ctatttggga tggtaagatt aaagattaaa
     3661 ttgcaaaagg ggataaacaa tacatttatc aaattaaagt acagttgaat aataaaggtg
     3721 tcggtggcga tggtgtccaa atttatcaat actatatcaa aatggacaac aataaacctt
     3781 acttaagtcc caaagataag actactgtag agaagttaga agatcgctgg aaaaaaatta
     3841 ctttcaaagt tcaggatact ggcattggtt tgaaagacgt ttatcttcaa tctgttaagt
     3901 atgttggtgg tggcaataat aatttagacc ttatcacacc tccaggattt aaaaaagaag
     3961 ataaaaaagt tgaaaaacca aaattagacc gtccaccagg aattgattta ccagcaccaa
     4021 cttcaatgag aagttttgat tattcaaccc caccgggaac taagccaagc aaacccaaag
     4081 atagtttatc aactcctcca ggtttcccag atttaaacac gccgccggat gaagccacca
     4141 aaggatagta aaaaagacgc tattgaagat aaatcaggag caattaaata tgctaagtct
     4201 cttcaactta gctttgttga tgaccctatt ttagctagca aagtaaatgg caaaatatta
     4261 caagtcgaat ctgatggcaa attagtcatt cctagaaatg ctttgtcagc taatcaattt
     4321 gatgacacta gtcttaaaat ttatcgtaat aataatcgca ataaagaaat tactatcaca
     4381 acagattatt ttgcagatac aaaatatgtc aatatcacag cggttgacta tttgagcaat
     4441 actacttttg agcaattagc tactggtgaa acagtagatt accatgccat tgtattttca
     4501 agctttgctg ctattaaaga caagggtggt aagatttatg ttaacgataa attgcaagaa
     4561 acttctcgta tagcgcttaa agataaatct gttaagattg gtattgaatt accaaatgat
     4621 gtcagacata ttgatagttt atctgttcgt cgtttgaatg aggttaaaac tgttgataat
     4681 atcttgaaaa atgatgaaca agacattaat ctcagcaaaa cttaccaatt aaaatacaac
     4741 ccgacaaatc gtcgtctaga gtttactatt aataacatta actcaagttc agaaatcatg
     4801 accactttca aagatggaaa gatgccagaa ttggttgaac aaaaagatgt ttctttggat
     4861 ataaacgata tggacatgag taagtttaaa actattcgac ttggacgaaa ggattctgaa
     4921 tttaagggac aacttattgc aaaaactgga acagttgaat tagatatgtt tttcaaacaa
     4981 tctcaagacc cagcttcaat tattaaaaaa atatacctta tccaaaatgg tgttccaaat
     5041 gaattgaaaa aatttgactc tagttttggt ttaactgaaa gtcagataga tggatactat
     5101 atttataaag atgcaattaa ccttaaattt aaattaacca gtggtgcaag tcttaaagtt
     5161 gtttataaag ggcaagaaga tccatatagt catcagaaag aagatatgac taaaaaaggt
     5221 gaacagctca gtcattcaac tcaagccaat gaaaatacag caaaagtaac ctttgctaat
     5281 attgactggt cacattatag taaggttact gtgaatggaa aagaagttgg taaaggtagt
     5341 gagttacctt taactaaagg atggacaaca tttgtattac ataaaacaga aaattcatta
     5401 aatgttaaaa gtttgattat ggagacgggt agtgtaagta agaaagttca acaacttcct
     5461 ttaagtccta gattatctaa aaataagcat atgagggata tgctacttac tatgcaaaaa
     5521 gattcagcgt attacgaaac aagtgacagt ctagtccttc gaattaatct cactgcagat
     5581 actaaactta attttaatgc tgttaaagga gcgagtgctc ttactgaaaa tatgatgatg
     5641 agacagtttg cagttgctgg accacaagat gatcctgtta gtgaacataa atacccatca
     5701 gtatttctct taactcctgc cttattggaa actgctagtg aggcaactct aaatggtaag
     5761 gaaatcacag catctggtat tatcggtcac atcaaggatg gtgataaaag caagcatgtt
     5821 gaagtcaaaa tggtgaatga aaatggagac atgctaggaa cccctgttat tattcaaggt
     5881 aaagacttga ctaatcgaac aaaaccatta atgagtggac gtagagtact ttatgccggt
     5941 aaacaatatg agttccgggc taaattacca cttagtcgtt ttaacacttg gattagggtt
     6001 gaagtggtaa cagaagcagg agagaaagca agtattgttc gtcgcatgtt ctttgaccaa
     6061 tcagttccag agcttaacac agcagttgct aaacgtgatt tgacttctga tactgctctt
     6121 atccacatcg ttgccaaaga tgactctcta aaactaaaat tatatcaaga tgattcatta
     6181 cttgaatctg ttgataaaac cggtctttat agttttagaa atggtgtaga aatcactaaa
     6241 gatatgacag taccactaga atttggagat aatattatta agttatctgc tgttgactta
     6301 tcaaattatc gtcgtaatga gacccttcat atctatagaa accgttttga tgttaaagca
     6361 agccaaatga cagctgacaa aggagctaaa gtaactgtgg atatgttgat gaagcactta
     6421 gttgttccag aaatggcagg agcttataca ttaacaatcg acgaagctcc aaacacaaat
     6481 gaatcaggaa tgttaacaaa cgctaaagta tcgattcatt atgtaaatgg tggtgttgat
     6541 aaagttgatg ttccgattaa agtagttgac ttagaagcta ttcgtaaagc tgaagaagca
     6601 cataaagctg acgaagcacg taaagctgaa gaagcacgta aagctgaaga agcacataaa
     6661 gctgaagaag tacgtaaagc tgaagaagca cataaagtcg aagaagcacg taaagctgaa
     6721 gagggacata aaacccaaga agcacctata gttgaagaag gctacaaagt taataacgtt
     6781 catcaaactg atactacagt taaagcgtct gatttaccaa agactaagac agtttccgca
     6841 gttcatatgg ctagaacaga caataaacag ataacttcac atcagacaca tgttgaaaaa
     6901 caaattaaaa atacattgcc atccactggt gacagcaaac gtggttatta tatcactgga
     6961 atggctatcg ttatgctgag tgtattattt agtttagcta aaaagtttaa aagcaaa
//
LOCUS       DATRNX010000092         6920 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRNX010000092 DATRNX010000000
VERSION     DATRNX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            66..1181
                     /locus_tag="VBC81_001588"
     CDS             66..1181
                     /locus_tag="VBC81_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6912615.1"
                     /translation="MSDDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKD
                     DIDVWVGGKSYNPARSFRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILF
                     ELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILER
                     MYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDI
                     AELYQKYSKEELANLGINIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLE
                     PTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYY
                     FQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            1502..1951
                     /locus_tag="VBC81_001589"
     CDS             1502..1951
                     /locus_tag="VBC81_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6912616.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            2153..2473
                     /locus_tag="VBC81_001590"
     CDS             2153..2473
                     /locus_tag="VBC81_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912617.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2473..3573
                     /locus_tag="VBC81_001591"
     CDS             2473..3573
                     /locus_tag="VBC81_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6912618.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3570..4175
                     /locus_tag="VBC81_001592"
     CDS             3570..4175
                     /locus_tag="VBC81_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912619.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4381..4986
                     /locus_tag="VBC81_001593"
     CDS             4381..4986
                     /locus_tag="VBC81_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6912620.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            4979..5227
                     /locus_tag="VBC81_001594"
     CDS             4979..5227
                     /locus_tag="VBC81_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6912621.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5317..6582
                     /locus_tag="VBC81_001595"
     CDS             5317..6582
                     /locus_tag="VBC81_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912622.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         2452 a         1258 c         1167 g         2043 t
ORIGIN      
        1 attatttttt cagttctatt ttatatagag atttagaggc tgtagatgaa aaactatatt
       61 ctttcatgag tgatgattta atttcaatcc aatcaagttg tctaagtatt gccaagacat
      121 ttgcaaattt caatattcaa agaaaaagat ttatgatttc tactgaaact gaaatggata
      181 cttttcacaa gaaagatgac attgacgtat gggttggtgg aaaaagttat aaccccgcta
      241 gaagttttag aacccgtacc atcaatgaat tgactgtcat tgattttgaa gaaatgtttg
      301 atattctttg gttgatgtta ggggataatt taattaaatc atttgaagtt aatgtctgtg
      361 gtatcctatt tgaattaggc ggaaatgaca taccctcaac atttaggcaa gaaaacattg
      421 atcctctcat caataaatgg tggtatgata atgtttcgac agaaattatc cctaatctaa
      481 taaaaaaact caaagaaaat cccttgttta atatcggatt tatggtaaat gatatattag
      541 aaagaatgta taaagagaat attccaaaat cttaccttac atccgtccca ttagttattt
      601 ctcaaaaagg aagaacaacc tattcgttta gtatgactgg tggtcaacaa atagatggag
      661 tgaaattcac acagatatat gaggactata tgaaattact cagtcaaggt aaggatatcg
      721 cagagttata tcaaaaatat tctaaagaag agttggcaaa tctaggcatt aatatttatc
      781 aatccaatga tatagaaagg actgaggaaa gaacttttga tgaaattatc agttgggttt
      841 ccaaccctta tgcaacaaga ccaattcaag aaaggcacac tattcaatta gagccaacaa
      901 gattttcact agaggataag aaaagaattg aagaagctgc agctcaagga ctaagcgaaa
      961 tcgaccttat tgatttagtt gacctatatg atattaattt agacaataca agcgtcaatc
     1021 gccatattgt ggggttattg actaataaca cccaagtaac atactatttc caagaacaat
     1081 taaataagga gttgctgtca atggctcacg ctttagataa cgtacaacag gcctttatta
     1141 aattattaag tgaagaggag atacgaaaat ttgctcttta aagactaggt gaatcgccta
     1201 gtcttttttt gcgcctcgtt ttacaaatta gtctattgaa gtaaaaaaag aagtaaaaaa
     1261 taaaagaaca ttacgcctta tactgaatat atcaagaaaa actcttgata taaattttaa
     1321 tagtccaaat tgatggccat caaaggacag attgaaacaa atatgatgac atgacgataa
     1381 aggtcatgtc ttgcatagaa ttgtttcgct gtttctttgt tgtgcctttt ggtatactca
     1441 gcgttcaata tttgctcatt ttgtttcctt ctgtccaaag gacagaaagg agctcatcat
     1501 tatgagtaat aaagtacaag aacgtcgtga gcgtaaaatc aaagaagcta ttaaagctaa
     1561 aaattgggat gaagtaactc gtttgcttca acaagaacaa agtaacgcag aacgtcgtga
     1621 ccgataccac aatagacgaa tcaaagacga aacaatagct agcaaaaatg ccaaaaagtc
     1681 tgtacgttat gatgtcattg caagttctga tttgaatccc gaagaagctt taatcctaga
     1741 ggagttaaga caagctattc gtgaagctaa agcatcccta tcagaaattg acagtaagat
     1801 tgttgaaatg atagcggaac aaggttcaag ctataaagaa acggctcgct atatcactga
     1861 acattataag aaaatgagtg atgtaactgt taaatcccat tattgtaaag cgctaaaaaa
     1921 attagctcct ttattaaaag cttaccgcta atccctcaaa tccagtgtct ctgtcactgg
     1981 attttttgta aaaaaaaatt aaaaattcaa caaataactt tgcgtttagc tgtctcattc
     2041 tccttagtaa tgaggaggtg gtcagataac ctaaaaaaca aagtccagct catgacccaa
     2101 accgagcctg tcgcattcac actatctctt attcataagg aggatttgcc ctatggcgtt
     2161 taaaatgaaa accacaaaag gtggttatac aactacattg gctgataaaa ttatcagtca
     2221 aaaacaaccg atttactcac ttagtaccga acttgaacca caacaacggt ttgaagaagg
     2281 aaaaccaacc ggagaaatcg tagcttataa agcatggttt gtacaggaag ggcaagaccc
     2341 tttccaagta aaatttgaag aaacgattga gcttccagct tttcaatcca tgattcaatt
     2401 tgacacacta caagcctgcg aagtaaaata taacatctac ttcaaagcaa atggtatcaa
     2461 ggaggttcgg taatgacttc acaatctcgt gatgtgctga accaatctta cctgcaatct
     2521 tacctgctcc agagcctaaa catggctctg ggggctttaa tgcagggaga aaccagctac
     2581 accaattctt ttaatattgt tattcaagca gacggcttta tctttgtccc tcgtctacca
     2641 tgtgcttata tccttgatga tgacttgtac aagaaaatct ttctgattgc caatgcttca
     2701 ctttatccac aatatacgct tctaaaacaa aacgctacct attttgtccc tctagaaaca
     2761 gatgacttac acactcagcg tggattattt ttcccttgga aaaagggaat ttcagaacgt
     2821 ttagtcattc cagatttgga taagttttca gctagcttgc cacaggggaa aatccctatc
     2881 atgaagtact ttgaactcaa tttagataag gttaatcact gggctattgc tggaaattca
     2941 ggttctggga aatcgtacgc tctcacttat ttcttgagtg tactaaagca tatgtctgac
     3001 ttgattatca ttgaccctaa atttgatacc ccaagtcgat gggcaagaga aaatcacgtt
     3061 gctgttatac accctgttga aaaccgttca aaatcagatt ttgtctcaca ggtcaatgaa
     3121 caattaagtc agtgtgcaac tctcattcag aaacgacaag ctatcttgta tgataatccc
     3181 aatcatcaat ttacccatct aactattgtt attgatgaag tcctcgctct atcagaggga
     3241 gtcaataaga atatcaaaga agcctttttc tccctactct cacagatttc cttattagga
     3301 cgtgctacca aaatccatct gtttttggta agccagcgtt ttgaccacaa tactattcca
     3361 atttcagtga gggaacagct aaacgtattg ttgcaaattg gaaatatcaa tcagaaaact
     3421 actcaatttt tatttccaga cctagaccca gagggtattg ttattccaat aggacatggg
     3481 acaggtatca ttcaagttat cgataatgaa catccctacc aagttctgcc cttactctgt
     3541 ccaacctact acactaaacg aggtattcta tgaccaataa aacctacttt atgagaagtt
     3601 ttaatctcat actatattta tttctatttg cacctatctg ttctgcattt ggatggttat
     3661 tcaattatct ttccgttcaa ttaacaaata tcacgtttgt caatttagat acggtcaaaa
     3721 gtattacgga tatcattagt tccgtttgtc atggatttgc acttatttct cttctacttg
     3781 ccttattttt ggttggatta gaaataatac aacggttgaa aacagatagt ctttggaact
     3841 atatccaatc tgtctatcac acttttctgt tgcgacactt cctgtttcag cgtgaacgag
     3901 tacagaagat aagcaatctt gaacatcaga cggttactac tatcaaccca atccataacg
     3961 gatttaaccg agctgttcgt aagtgtatcg tagatatacg aaaagatacc atcactattt
     4021 ttctaaaagt ccctagagac caacaagggc aaaaaatcct aaaagagatg gaagcacaat
     4081 taaaagaaga aatcgtcagc caacacgcag actattattt ctcctctcct attcgagttc
     4141 gtaaccaatt gtggttcact ggacaaaaac gttaatccct tggggctgtg gcttgcgtta
     4201 agcaaaagcc agacagcccc tttttatatt cttttctatc atgacctatt ggggtgacta
     4261 cgacccccaa taggtcaata atcaataatc acaaaaaatt caattttttc aaaaaaactt
     4321 tgcatacgac ctgtcatttc tccttagtaa tagaacccaa aagaaatgag gtaactgcct
     4381 atggcaaaag aacagcgctc aaccaaatgg acttttctct tctacgagga aagcgcccca
     4441 gagaactact tgaatatatt agaggaactc catattccgt ttattcttag tccttggcac
     4501 gataaggacg ttaatagaca gacgggagag ttcaagaaat cgcacaaaca cggtgctttc
     4561 ttctttgatt cactgaaaag ctattcacaa gtatcaaata tcatcagtga caaactaaac
     4621 ggtccagcac atgtggaggt agttatgtct cccactggtt tatttgacta ctttacccat
     4681 gcggaaaatc cagataagac cccctacaac attgaggata ttgaagtcgg ttgcggcttt
     4741 aatttagaaa aattcttgat ggagatgaac tcctcggatt ttatacatga agtagttgat
     4801 attatcgaag agaacgactt taccgaattt gaagaattgg tctggtatgc ccgagcaaac
     4861 aataccaatt tattagggct tatcattgaa cgtacatatt tcttcgctaa atacctagat
     4921 tcacgaagat acaatcctaa tcgactccat aattcaaaca cagaggagaa agagaataat
     4981 gaataacgaa aaattagaac aaattgaaca attattgcaa acacttatca aactcattca
     5041 aatcaaagaa cagaacatac cactaaagat aatacaacag cgagaattgc taaaacagct
     5101 aaatatctcc ccaaacacac taaaaacttg ggaacaaaaa ggattaaaaa gattagaacc
     5161 tcctatagag ggaactcgaa ccgtcttcta tctactagat gatattatca actttttaca
     5221 atcctaattc tgaaaatttg tgctaaaata gttttatcta aaagaaagag tggtaactat
     5281 tttagcctac ttcaatatcc atgaagaaag gcaaatatgg aaactgaaac aattattcat
     5341 aatggcaaaa aagtcattaa aaaaatcaaa aaagataagt ccatctcata cactttgaag
     5401 gggacatttc tgggaaaaga tatcaaaact ggtaagcaag tcactactac cattacagcc
     5461 aagacactaa aacagttaga tagagcaatc attcaagcac gactagaatt tgaaaaaaat
     5521 ggctcaacac gagaaaaagt tatcgtcatt gatacactgg aagatttagc tgaagaatgg
     5581 tttaaatcat ataaaacctg ggttaactcg cataatactc tgaacagagt aagaggttat
     5641 cttgataatt atattatccc taaatttgga gattacaaac cagataaaat tgaatctgcg
     5701 gatatccagc tttggttaaa cgatttggca aagaaatcaa aagagtccat tgaatctggt
     5761 ataaagcgtg ctgataaagg tagtgctaaa gattttggtg ctgttatcca taagttgaaa
     5821 gatatttttg actatggaat tacaaactat ggactaatca ctaatcctgt tagcactgtt
     5881 aagataccac ctaagccaaa atcaaataag caacgcatca tggtattaca tgatgatggt
     5941 cttgtaatat ggcttaatta tcttgaaagt ttagataaca atagagccaa tcgtagattt
     6001 aagttaattt gtaatactct attagcctcc gctttgcgaa taaatgaatt acttgcattg
     6061 acgattgatg acctagattt tgaaaattct tctattaacg tcagtaaaac attgatgtgg
     6121 aaaaccgcaa ataaaaaaga tgggacaaaa ggggaagtta tctgtaaagc tacaccaaaa
     6181 actgatgcag gaaatcgctc tgtccctgtc cctcccccca taatagaaaa actcagagat
     6241 ttccaccacg aaatgaacaa gtatcttgaa ttgcacaaca gacatagaac aaaactgatt
     6301 ttccctacaa tctatgggaa ctatatgtgt gacagaaacg agagaacaac tcttaagaaa
     6361 aggttagtag gtcttggatt accaaactat ggtttccatt tattccgaca tactcatgct
     6421 tcaatgctac ttaatgctgg aacaaattgg aaagaactcc aagtcagaat gggacataaa
     6481 tccatttcaa ctacaatgga tacttacgct gaattagcac cacaacgaaa gttagaagct
     6541 gtggggattt atcttgaaaa gattgctgag ctaacgcaat aactactcta ccattcactc
     6601 taccttttat tatgtagcat tctagaaaca ttgatgtacc aacgtttcta acagctaaaa
     6661 tagaaattat ttaactctta ctgaagttaa ataaggattc attacacgtc aataggcagt
     6721 aaaaagattg gttttatgag tttttgaaat tttatatttc actacactgc aatataaatc
     6781 agttgaatct ctacattttt ctctacattt tggtaagaaa aaaatgacgg ataactaaac
     6841 cccactagca agagttagtg gggttttatt attatttata aatcaattta ctttatttca
     6901 ataatctatt ataaattata
//
LOCUS       DATRNX010000093         6873 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRNX010000093 DATRNX010000000
VERSION     DATRNX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..200
                     /locus_tag="VBC81_001596"
     CDS             <1..200
                     /locus_tag="VBC81_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6912623.1"
                     /translation="FDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLISRDDY
                     LKAELILRREHRLELEKENKK"
     gene            197..1165
                     /locus_tag="VBC81_001597"
     CDS             197..1165
                     /locus_tag="VBC81_001597"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6912624.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            1177..1557
                     /locus_tag="VBC81_001598"
     CDS             1177..1557
                     /locus_tag="VBC81_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6912625.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            1789..3630
                     /gene="typA"
                     /locus_tag="VBC81_001599"
     CDS             1789..3630
                     /gene="typA"
                     /locus_tag="VBC81_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6912626.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            3675..3920
                     /locus_tag="VBC81_001600"
     CDS             3675..3920
                     /locus_tag="VBC81_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6912627.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            4050..5405
                     /locus_tag="VBC81_001601"
     CDS             4050..5405
                     /locus_tag="VBC81_001601"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6912628.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            5408..6484
                     /locus_tag="VBC81_001602"
     CDS             5408..6484
                     /locus_tag="VBC81_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6912629.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            6488..>6873
                     /locus_tag="VBC81_001603"
     CDS             6488..>6873
                     /locus_tag="VBC81_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HEO6912630.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITV"
BASE COUNT         2281 a         1065 c         1406 g         2121 t
ORIGIN      
        1 tattcgatgt gcagaatctt ttgaaaaaat ttggtattta tgtttatatt ggtaaacgtc
       61 tttatgatat cgaagttatg aagattgaat tgcaacgtct ctacgataat ggtctgataa
      121 gtagagatga ctatttaaaa gcagaactga ttttaagaag agagcacaga ctagaattgg
      181 agaaggaaaa taaaaaatga gtaagaaatt attgggaatt gacctcggag gaacgaccat
      241 taaatttggt atcttgacgc ttgagggaga agtacaagaa aaatgggcaa ttgagaccaa
      301 tactttagaa aacggaagac atatcgtttc tgatatcgtt gaatctctca aacatcgttt
      361 gagcctctat ggattaacaa aagatgactt tctcggtatc ggtatgggtt ctccaggagc
      421 tgttgataga actagtaaaa cagtaacagg tgcttttaat ctaaattggg ctgatactca
      481 agaagtaggt tcggttattg aaaaagaagt tggaattcca ttttttattg ataacgatgc
      541 taatgttgca gcacttggtg aacgctgggt aggtgctggt gccaataatc ccgatgttgt
      601 tttcgtaacc ctcggaacag gagtaggtgg aggtgttatc gcagatggta acctcatcca
      661 tggtgttgca ggagcaggtg gagaaattgg gcatatgatt gttgatccag aaaatggatt
      721 tacgtgcaca tgtggtaaca aaggctgtct tgagacagtt gcatcagcga caggtgttgt
      781 tagagtagca cgtcaactcg cagaacaata tgaaggttcg tctgccatta aagcagcgat
      841 tgacaacggt gatactgtta caagtaaaga tatttttata gcagcagaag atggggataa
      901 atttgctaat tctgttgttg aacgtgtatc acgttacctt ggactggcag cagctaatat
      961 ttcaaatatt ttaaaccctg attctgtggt tattggtggc ggtgtctcag cagcaggtga
     1021 atttttacgt agtcgcgttg agaaatactt tgtcacattt gctttcccac aagttaaaaa
     1081 gtcaactaaa attaagattg ctgaactagg taatgatgct ggtattattg gtgcagcaag
     1141 cttagccaat caacaagcaa gttaaggaga taagatatgg atatgtctgt tattttaatt
     1201 atcgttattc ttctggcttt tgttgcttgg gcatcttgga attattggag ggttcgtagg
     1261 gcagctaaat ttttagataa cgagtctttt caaaaagaaa tgtcacgagg acagttaatt
     1321 gatatccgag aagctggtgc ttttcataga aagcatattc tcggagcacg taacattcca
     1381 gcaagtcaat ttaaagttgc cttatcggct ttacgcaaag ataaacctgt cctcttatat
     1441 gacgcaagcc gtggtcagtc tattccaagg attgttttgt tactcagaaa agaaggtttt
     1501 aatcaacttt acgtgttaaa ggacggtttc aattactgga cgggtagagt taagtaatat
     1561 actaaggaga gagttactat taataactct cttctctttt tgtagaaatg aattaaattg
     1621 aaaaaagtag aaaataaatg acataaataa tgaaatgatg aaaagttttc ctatcacaaa
     1681 taggcagtta atatgaaaac atttacactt gtgtaaattc tgttttttaa gaaaaattgt
     1741 gttataattc ataagttaac agaattacat tataaaatag aggaaaacat gacaaattta
     1801 agaacagata tccgtaacgt tgcgatcatt gcccacgttg accacggtaa aacaactctc
     1861 gttgatgaat tattaaaaca atcacatact cttgatgagc gtaaagagct tgaagaacgt
     1921 gcaatggatt caaatgatat cgagaaagaa cgtggtatca ccattcttgc aaaaaataca
     1981 gccgtagcat acaacgatgt tcgtatcaat attatggaca cacctggtca cgcggacttt
     2041 ggtggtgaag ttgagcgtat tatgaaaatg gttgatggtg ttgttttagt cgttgatgct
     2101 tacgaaggaa caatgccaca aacacgtttt gttttgaaga aagctcttga acaaaactta
     2161 attccaatcg ttgttgtaaa taaaattgat aagccatcag ctcgtccatc agaggttgtt
     2221 gatgaagttc ttgaactctt cattgagctc ggtgctgatg atgaccaact agatttccct
     2281 gttgtttatg cttcagccat caatggaaca tcttcaatgt cagatgatcc ttcagatcaa
     2341 gaaaaaacaa tggcaccgat ttttgatact atcattgatc acattccagc cccagttgac
     2401 aactcggaag aaccacttca attccaagtt tctcttcttg attacaatga ttttgtagga
     2461 cgtattggta ttggacgtgt tttccgcggg actgtcaaag ttggagatca agttactctt
     2521 tcaaaacttg atggtacaac taaaaacttc cgcgtaacaa aactttttgg tttctttgga
     2581 cttgaacgta aagaaatcca agaggctaaa gcgggtgatt taatcgctgt ttctggtatg
     2641 gaagatatct tcgttggtga gacagtaact ccgacagatg ctattgaacc actaccagtt
     2701 ttacgtattg acgagccaac acttcaaatg actttcttgg tgaataattc accatttgca
     2761 ggtcgcgaag gtaaatggat tacgtcacgt aaggttgaag aacgtctttt agcagaatta
     2821 caaacagacg tttctttgcg tgttgaccca acagattcgc cagataaatg gacggtttca
     2881 gggcgtggag aattacattt atctatcctc attgaaacaa tgcgtcgtga gggatatgaa
     2941 cttcaagtat cacgtccaga agttatcatc aaagaaattg atggtgttca atgcgagccg
     3001 tttgagcgtg ttcaaattga tactccagaa gaatatcagg gtgctattat ccaaagtttg
     3061 tcagagcgta aaggtgatat gcttgatatg cagatggttg gtaatggtca aacgcgtttg
     3121 attttcttga ttcctgcacg tggtttgatt ggttattcaa cagagtttct ttcaatgaca
     3181 cgtggatatg gtatcatgaa tcatactttt gaccagtatc taccggttgt tcaaggagaa
     3241 attggtggtc gtcatcgtgg tgtcttggtt tctattgaaa atggtaaagc aactacatat
     3301 tcaattatgc gtattgaaga acgtgggact atctttgtaa atccaggtac agaagtttat
     3361 gaaggaatga ttgttggtga gaattctcgt gataatgatc tcggagtcaa tattacaact
     3421 gctaaacaaa tgacaaatgt ccgttcagca actaaagatc aaactgcagt catcaagacg
     3481 cctcgtatcc ttactttaga agagtcactt gaatttttag cagatgatga gtacatggaa
     3541 gtaactcctg agtctattcg tcttcgtaaa caaattttaa ataaagcagc acgtgacaaa
     3601 gctaataaaa agaaaaaatc agctgaataa tatccaaagt atttcttatt tatgattaat
     3661 aagaaaggag ttctatgttt tacttaattg ttgccctttt gattgcctta tattactttt
     3721 ttatggcacc aaaaacagta cgtaatactt tgaacgctat tggtttagta ggtttggtag
     3781 ccttattact tgtcttggca gttatgagtt ttattaaaat ccttcaattg ccaggtgaat
     3841 tgtatattgg gcttataatg attcctctag gatatacagc atttaaagaa acattgaatt
     3901 tgtcagaaaa gaagaaatag cttatgaact taggattttg tctcctaagt tcttttgttt
     3961 tgagaaatta gagacatatc ctttaccttg aaaaagccac tgattaaagg taaaataata
     4021 gggataataa tgatagaatg ggacgagtaa tgaaaacaat aacaacattt gaaaataaaa
     4081 aagttttagt ccttggttta gcacgatctg gagaagccgc tgcacgtttg ttagctaagt
     4141 taggagcaat agtgacagtt aatgatggca aaccatttga tgaaaatcca acagcacagt
     4201 ctttgttgga agagggtatt aaagtggttt gtggtagtca tcctttagaa ttgttagatg
     4261 aggatttttg ttacatgatt aaaaatccag gaatacctta taacaatcct atggtcaaaa
     4321 aagcattaga aaaacaaatc cctgttttga ctgaagtgga attagcatac ttagtttcag
     4381 aatctcagct aataggtatt acaggctcta acgggaaaac gacaacgaca acgatgattg
     4441 cagaagtctt aaatgctgga ggtcagagag gtttgttagc tgggaatatc ggctttcctg
     4501 ctagtgaagt tgttcaggct gcggatgata aagatattct agttatggaa ttatcaagtt
     4561 ttcagctaat gggagttaag gaatttcgtc ctcatattgc agtaattact aatttaatgc
     4621 caactcattt agattatcat gggtcttttg aagattatgt tgctgcaaaa tggaatatcc
     4681 aaaatcaaat gtcttcatct gattttttgg tacttaattt taatcaaggt atttctaaag
     4741 agttagctaa aactactaaa gcaacaatcg ttcctttctc tactacggaa aaagttgatg
     4801 gtgcttacgt acaagacaag caacttttct ataaagggga gaatattatg tcagtagatg
     4861 acattggtgt cccaggaagc cataacgtag agaatgctct agcaactatt gcggttgcta
     4921 aactagctgg tatcagtaat caagttatta gagaaacttt aagcaatttt ggaggtgtta
     4981 aacaccgctt gcaatcactc ggtaaggttc atggtattag tttctataac gacagcaagt
     5041 caactaatat attggcaact caaaaagcat tatctggctt tgataatact aaagttatcc
     5101 taattgcagg aggtcttgat cgcggtaatg agtttgatga attgatacca gatatcactg
     5161 gacttaaaca tatggttgtt ttaggggaat cggcatctcg agtaaaacgt gctgcacaaa
     5221 aagcaggagt aacttatagc gatgctttag atgttagaga tgcggtacat aaagcttatg
     5281 aggtggcaca acagggcgat gttatcttgc taagtcctgc aaatgcatca tgggacatgt
     5341 ataagaattt cgaagtccgt ggtgatgaat tcattgatac tttcgaaagt cttagaggag
     5401 agtaactatg gggaaaaaaa ttgtttttac aggtggtggt acggtaggtc atgtaacact
     5461 aaacctaata ttaatcccca aatttatcaa agatggttgg gaagtacatt atattggtga
     5521 taaaaatggc atagaacatg aacaaattaa tcagtctgga cttgatatca catttcactc
     5581 tatagctact gggaaactac gtcgctattt ttcatggcaa aatatgttag atgtctttaa
     5641 agtaggggtt ggcgttcttc aatcgattgc tattattgct aaacttagac cccaagcctt
     5701 attttcaaaa gggggatttg tttcagtacc tcctgtagta gctgcacgcc tattaaaagt
     5761 tcctgttttt gttcatgaat cagatttatc aatggggtta gcaaataaga ttgcttataa
     5821 atttgcgact attatgtata cgacttttga acaatctaaa gatttaatca aaactaagca
     5881 tattggtgct gtaacaaaag tcatggattg taaaaagtcg tttgaaaata ctgacttaac
     5941 tagtataaaa gaagccttcg atccaaacct aaagacctta ctatttattg gaggatcagc
     6001 aggtgcaaaa gtattcaatg attttattac tcaaacacct gagctggaag aaaaatataa
     6061 tgttatcaat atttcaggcg attcttctct caatcgattg aagaagaacc tttatcgcgt
     6121 ggattatgtt acagaccttt atcagccttt aatgaattta gcagatgtgg ttgttacacg
     6181 aggtggctct aatactattt ttgaattagt agctatgaaa aaacttcatc ttattatccc
     6241 tcttggacgt gaagcgagcc gaggtgacca attagagaat gctgcttatt ttgaagaaaa
     6301 gggctacgca ttgcagttac cagaatctga attaaacata aatacacttg agaaacagat
     6361 aaatttatta atttctaaca gtgagagtta cgaaaaaaac atgtcacaat catctgaaat
     6421 aaaatctcaa gatgaatttt atcaattatt aattgatgat atggcaaaag taacaaaagg
     6481 ataaaacatg cctaagaaga aatcagatac cccagaaaaa gaagaagttg tcttaacgga
     6541 atggcaaaag cgtaaccttg aatttttaaa aaaacgcaaa gaagatgaag aagaacaaaa
     6601 acgtattaac gaaaaattac gcttagataa aagaagtaaa ttaaatattt cttctcctga
     6661 agaacctcaa aatactacta aaattaagaa gcttcatttt ccaaagattt caaaacctaa
     6721 gattgaaaag aaacagaaaa aagaaaaaat agtcaacagc ttagccaaaa ctaatcgcat
     6781 tagaactgca cctatatttg tagtagcatt cctagtcatt ttagtttccg ttttcctact
     6841 aactcctttt agtaagcaaa aaacaataac agt
//
LOCUS       DATRNX010000094         6841 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRNX010000094 DATRNX010000000
VERSION     DATRNX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..422
                     /locus_tag="VBC81_001604"
     CDS             <1..422
                     /locus_tag="VBC81_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912631.1"
                     /translation="ENILATLKIRFLYSIKLNFPIFVALNICFGTYYFNFFTPKIDQI
                     IILVITQAISMILFSFYFLYLYFLLQPFTEGLKSKSAMYNILTFIIYYMGYKLFTFTK
                     SITMPILLVSLGIIVVILIIGYLAVVVFAPKTFRLKS"
     gene            618..1787
                     /locus_tag="VBC81_001605"
     CDS             618..1787
                     /locus_tag="VBC81_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6912632.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1784..1930
                     /gene="rpmG"
                     /locus_tag="VBC81_001606"
     CDS             1784..1930
                     /gene="rpmG"
                     /locus_tag="VBC81_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6912633.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1977..2213
                     /gene="secG"
                     /locus_tag="VBC81_001607"
     CDS             1977..2213
                     /gene="secG"
                     /locus_tag="VBC81_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6912634.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2326..4731
                     /gene="rnr"
                     /locus_tag="VBC81_001608"
     CDS             2326..4731
                     /gene="rnr"
                     /locus_tag="VBC81_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6912635.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4734..5201
                     /gene="smpB"
                     /locus_tag="VBC81_001609"
     CDS             4734..5201
                     /gene="smpB"
                     /locus_tag="VBC81_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6912636.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(5285..6673)
                     /locus_tag="VBC81_001610"
     CDS             complement(5285..6673)
                     /locus_tag="VBC81_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6912637.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
BASE COUNT         2295 a         1070 c         1359 g         2117 t
ORIGIN      
        1 gagaaaatat cttagcaacc ttaaaaatac gtttccttta tagtataaaa cttaatttcc
       61 ccatttttgt agctttaaac atatgttttg gaacctatta ttttaatttt ttcaccccta
      121 agattgatca gattatcata ttagtaatta cacaagctat atcaatgatt ttatttagtt
      181 tttacttttt gtatctttat ttcttattac agccttttac agaaggatta aaatcaaaga
      241 gtgctatgta taatatttta acctttatta tatactatat gggatataag ctatttacct
      301 ttactaaatc tataacgatg ccaatattat tagtaagcct aggaattata gttgttattt
      361 taattattgg ctatttagca gttgtagtat ttgctccaaa aactttccgt ttaaaaagtt
      421 aattttaatt gtggtataat aacagaggca tgtgataaca taggtttagt tacataaagg
      481 aaattataat ggaaataaca tctcaaatag atgttaaatc tcaataaaac tgctaagata
      541 gttagtggta gaaaagttta gaaagaagat gatgttttat ctcagttgaa acggttaact
      601 ggaagcaaaa tttaaaagtg gcttggctcg gtaatttttt tactggggca agcttttcac
      661 ttgttatgcc ttttatggta ttatatgtag aacaattagg agcacctagc aacaaagtag
      721 agtggtatgc aggcctatca gtatccttat cagccttgtc ttcagcttta gttgccccat
      781 tatgggggcg cttagctgat aaatatggta gaaaaccgat gatggtaaga gcagggttga
      841 tgatgacttt tactatggga ggccttgctt tcattcatag tgttacagga cttctgattt
      901 tacgtatatt aaacggcatt tttgcaggct atgtacctaa ctctacagct ttgatagcaa
      961 gtcaagctcc tcaagaggaa tcaggatatg ccttaggaac cttagcgaca ggagtgactg
     1021 gaggtatgtt aattggtcca cttttagggg gacttttagc agagtggttt ggtattaggg
     1081 aagttttttt attagtggga accatattgt taatatctac tttaatgacc atttttatgg
     1141 taaaggaaga tttcaaaccc atttctaacg aagaaaccat gccaactaca gaagtgttca
     1201 aaagcgttaa aagcttacaa attttaatag ggctgtttgt aacaagcatg attattcaaa
     1261 tttctgcaca atcaattgca cctattttaa cactttacat tcgtcattta ggacagactg
     1321 aaaacttgat gtttgtttcc ggattaattg tttctggtat gggattctct agtattctga
     1381 gtagtccaaa gctaggaaga atcggggatc gcataggcaa tcatcgtttg ctattattag
     1441 ctctactgta tagtttcctc atgtatgtct tatgttcatt ggcacagact tcattacagc
     1501 taggggttat tagattcctg tatggttttg gaacaggtgc cttgatgccg agtattaatt
     1561 caatcttgac taaaattgct ccaagacaag gtttatcaag gattttcagt tataaccaaa
     1621 tgtttagcaa tttaggacaa gtattaggtc cttttgtcgg ttcagcagtt tctattcatt
     1681 taggattccg atgggttttc tttgtgacta gcttcatcgt tttagctaat tttgtttggt
     1741 gttttattaa ttttagaaaa tatattagag ttaaggaaat tgtgtgagag ttaaaattaa
     1801 tttaaagtgt agctcttgtg gtagtaagaa ttatttaacc agtaagaata aaagtaatca
     1861 tcccgataaa atcgaggttc caaagtattg cccaaaggag agaaaagtaa cactacatat
     1921 tgaatcttaa taagttttat gttagaatgt actttgacca tagtggagga attaacatgt
     1981 ataacctatt actcaccatt ctacttgttt tatcagtatt attgattatc tcaattttca
     2041 tgcaaccaca aaagaatcca agtagtaatg tttttgatag tagcggttct gaagcccttt
     2101 ttgaacgtag taaagctcga ggatttgaag cttttatgca acgttttaca ggtgttttag
     2161 tctttttctg gctactaatc ggcttagtat tatctatttt atcaagtcat taatgatagc
     2221 taaatacaga taagtgagga tgggggatag cccaacctca ctttttgtta gtagaatggt
     2281 tgtagagtaa taataaagtg actaaagtta ctagaaaagg aaataatgaa agaaaaaatt
     2341 ataaaatatt tggaagaaca cggtaaaacc agcgttaatg atttagctgc tgccctagat
     2401 atggcaggcg caaaagcatt tccaaaatta atcaaaacaa tctcaaattt ggagagccac
     2461 aggcaattaa gatttgacga taatggaagt ctttctcttc aaaagaaaga agctaagaaa
     2521 aaggaaatca cagtaagggg tctttttaga gcaaataaag caggatttgg ttttcttagt
     2581 attgaccaag acgaagatga tatgtttatc ggaaaaaacg atattgctta tgctattgat
     2641 ggtgacacgg tagaagctgt tgtaaagaaa ccagcggacc gtcttaacgg gactgcagca
     2701 gaagcgcgtg ttgttaatat tgttgaacgt agtttaaaga cgcttgttgg taagtttgtc
     2761 ttagacgatg agcgccctaa atacgcagga tatatcaaat cgaaaaatca aaaaatcaat
     2821 caaaagattt atatacgcaa agaaccggtc gttttagacg gaacagaaat tatcaaagta
     2881 gatattgata aatatccgac acgaggacat gattacttcg tagcaagtgt tcgtgatatc
     2941 gttggacatc aaggagatgt tggtattgat gttttagaag tcttggaaag tatggatatc
     3001 gtctcagaat ttcctgagga cgttattgca gaagccaatg ctatcccaga tgctccaacg
     3061 gagaaagatt taattgggcg tgttgattta cgtcaagagg tcactttcac tattgatgga
     3121 gcggacgcca aagatttaga cgatgctgtg catattaaat tactggataa tggtcatttt
     3181 gaacttggag ttcatatcgc agatgtatca tactacgtta ctgaaggctc tgccttaaat
     3241 cgtgaagcac ttagtcgtgg tacgtccgta tatgtcacag atcgcgttgt accaatgtta
     3301 ccagagcgtc tatctaacgg tatatgttca ttaaatccaa atcttgatcg tttaacgcag
     3361 tcttgtatta tggaaattga ccaaaatggg agagttgtta atcatcaaat tacacagtca
     3421 gtgattaata ccacttatcg tatgacttat atcgctgtaa atgatattat tgctggcgat
     3481 gaggaaatct gctcagaata tgagtctatt gtgtcctcag tacagcatat ggtcacttta
     3541 caccatactt tggaagctat gcgtacgcgt cgtggagcgc tgaattttga cacatctgaa
     3601 gcaaaaatta tggttaatga taagggaatg ccagttgata ttgttattcg caacagaggc
     3661 attgctgaac gcatgattga gtcatttatg ttggcggcaa atgaaacggt tgctgaacat
     3721 tatgctcgtc taaaactgcc atttatttat cgtatacacg aagagcctaa agctgaaaaa
     3781 ttacaaaaat tcatcgatta tgcttctgtt ttcggtgttc agattcaagg aacagcaact
     3841 aaaatcaccc aatctgcttt acaggacttt atgaaaaaag tacaagggca gccaggaagt
     3901 gaagtgttat ctatgatgtt attgcgctcg atgcaacaag ctagatattc tgaacacaac
     3961 catggacatt atggacttgc tgcggaatat tacactcact ttactagccc cattcgacgt
     4021 tatccagact tgttagttca tcgtatgatt cgtgattatg atgataaagc gatggataaa
     4081 gctgatcatt ttgctaacct tatcccagaa attgcaactc aaacgtctag tcttgagaga
     4141 cgtgccattg atgctgaacg tattgtagag gcaatgaaga aagccgaata tatggaagaa
     4201 tatgttggtg aggaatttga aggtgtggtt gcaagcgttg ttaaatttgg tatgtttgta
     4261 gaattaccaa atactattga aggattgatt cacgtaacga ccttgcctga atattatcac
     4321 tttaatgaac gtacgctgac gcttcaaggt gagaaatctg ggaaagtttt ccgtgttggt
     4381 caacaaatta aggttaagtt gattcgttct gataaggaaa ctggtgatat cgattttgat
     4441 tatttaccat cagattttga tattgttgaa aaggtaagta aaagctcgcg tgagggtcgt
     4501 cctaatagaa gttctaaacg agaacatcag catagaattt ctgatcgtga taataagaat
     4561 aaaaatacat ctaagaaaaa agcatctcga aaacccaaaa ggaactcgga tagcaaatca
     4621 catcatcata aagacgatag aacaacaggt tcaaccaaga aaaaaacgaa aaaacctttc
     4681 tataaaggcg ttgctaagaa aggtcagaaa cggaaatcta agaaaggtta agtatggtta
     4741 aaggtcaagg aaacgttgta gcacaaaata aaaaagcgca tcacgactat actatcgttg
     4801 aaacaataga agcaggaatt gttttgacag gaacagaaat caaaagtgtc cgcgcagcgc
     4861 gtattacatt gaaagatgga tatgcacaaa taaaaaatgg tgaagcgtgg ttgattaatg
     4921 ttcatattac accatatgat caaggtaaca tatggaatca agatcctgat cgtacacgaa
     4981 agttattact aaaaaaacgt gagattgaaa aaatatcaaa tgaactgaaa gggactggta
     5041 tgactctggt acctctaaaa gtttacttaa aagatggttt tgctaaagtt ctattgggtt
     5101 tggcaaaagg aaaacatgat tatgataagc gagagtctat caaacgtcgt gagcaaaacc
     5161 gtgatattgc ccgtcagtta aaaaactata attcacgatg attatcaaaa ggttagggaa
     5221 ttgcctaacc ttttttggac gaaaaaagtg aagttgattg accaacttca ctaataatat
     5281 agacttaatg tgatttatgt ttgaagtgac tatataaacc aaatccaata atccagatgg
     5341 ttgctccaat agccccgatg tatgttgaat cttgcaagaa gagagaaacg aatacaaaga
     5401 ggaagaagag aagtgtgatt ggtgaaagga ttttgtaggc aggcatttta taaccatctt
     5461 ccataaaatc aggtgattta cgatatttcc aatgtgcaat cataataaga acatagatag
     5521 aaatgtaaac accagaagat gatgcagtaa ttaaggagaa ggcatctgaa ataccaggta
     5581 aaacactgat aagtgctgaa gctccaacga ttacagctga tgcaataata gctcgacttg
     5641 gaacgctttg acgagataat tggtctaatt ttaacgcttt tgtaagagca tttggtgatt
     5701 cattagccaa ttggaataaa tgacgccctg ttgagtacaa agtagaatta agagcagatg
     5761 ctgcagacgt taggacaaca aagttaatca aagcggcagc ccacttaatc ccagctagtt
     5821 tgaatactgt aacgaaaggt gactcattaa ctggtaactg ttgccatggg aagatggcca
     5881 taattgctaa taatgaacct gcgtagaata aaataatacg catcggaatt tcttggattg
     5941 cttttggtaa aactttacgt ggattagctg tttcagaagt tgttactccg acaaattcaa
     6001 tagctaaata agcaaagaat accatttgga aagccatgaa gaaattcaat ttacctttag
     6061 ggaaccattc aaaatgattg gtgatatttg agattgaagc atggtatccg tgaccggtgt
     6121 caaagtttgt caataccata aagataccgg ttgctattaa accaagaatt gcaataactt
     6181 ttatcatagc aaaccaaaac tcggtttctc cgaatgcttt tacagcaatt aagttgatag
     6241 aacttaggat agctaaaaaa acaatttgga taatccagga tggccattca gggaaccaaa
     6301 attgaacata ctctcctacg gctgtaatct ctgccatacc gataaatatt agggaaatcc
     6361 aataagaaag tgttgagaaa aatccccaac cgggtcctaa atatttggta atgaaattaa
     6421 taaaagtatg ctgatttgga tcttgataaa gcatctctcc aatagcacgc atcatgaaga
     6481 acatgaaagc gccagtgata gcatagacaa gaactattga aggacccgtc aggctaattg
     6541 aacgacctgc accaagaaaa agcccagttc cgattgtccc tgcaatcgca attaactgta
     6601 cgtgacgatt ttctaggccg cgtaccatac cattctcaga tttttgagtg tggtcattat
     6661 tattttttga cattataaat actccatcta tttttgaaaa actccttcat tatataattt
     6721 ttttgcaaaa ttcgcaataa ttactataca atatttttct atgataaaat ctcctagcta
     6781 agtgagctaa gagatttcaa ttcatccctc ttccttgaca atatagactg ggcgtttttt
     6841 a
//
LOCUS       DATRNX010000095         6767 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRNX010000095 DATRNX010000000
VERSION     DATRNX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..2282
                     /gene="srr2"
                     /locus_tag="VBC81_001611"
     CDS             <1..2282
                     /gene="srr2"
                     /locus_tag="VBC81_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO6912638.1"
                     /translation="LKNSTIKIYKTTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWY
                     DIYWGQFIASNETYVIVVETPFTNAVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNA
                     QERKILSELVSSSESVSSSESVSNSESISTSESVSNSESISSSESVSSSESISTSESV
                     STSESISSSESVSSSESVSSSESISSSESVSNSESISSSESVSNSESISSSESVSSSE
                     SISNSESISSSESVSTSESISSSESVSNSESISSSESVSSSESISNSESISSSESVST
                     SESISNSESVSSSESVSTSESISSSESVSNSESISTSESVSTSESISSSESVSSSESI
                     SSSESVSNSESISNSESVSSSESVSNSESISSSESVSNSESISTSESVSTSESISSSE
                     SVSNSESISSSESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSSSESIST
                     SESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSSSESI
                     SSSESVSSSESVSNSESISSSESVSNSESISSSESVSTSESISSSESVSNSESISSSE
                     SVSSSESVSSSESISTSESVSNSECISSSKSVSNSESISSSESVSNSESISSSESVSN
                     SESISSSESVSSSESISSSESVSNSESILSSESVSSSESISSSESISSSESVSMSTTE
                     SLSESEVSGDSEISSSTESSSQSESMNHTEIKSDSESQHEVKHQVLPETGDNSASALG
                     LLGAGLLLGATKSRKKKKD"
     gene            2392..3645
                     /gene="secY2"
                     /locus_tag="VBC81_001612"
     CDS             2392..3645
                     /gene="secY2"
                     /locus_tag="VBC81_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6912639.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            3655..5232
                     /gene="asp1"
                     /locus_tag="VBC81_001613"
     CDS             3655..5232
                     /gene="asp1"
                     /locus_tag="VBC81_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6912640.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFF
                     TSLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAA
                     LSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGD
                     YTSGKILQRWRKLLEKE"
     gene            5234..>6767
                     /gene="asp2"
                     /locus_tag="VBC81_001614"
     CDS             5234..>6767
                     /gene="asp2"
                     /locus_tag="VBC81_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6912641.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWF"
BASE COUNT         2216 a         1056 c         1221 g         2274 t
ORIGIN      
        1 atttaaagaa ttcaacgata aaaatctata aaaccacctc ggataatatt gtagagagta
       61 tggtacagga ttattcaagc atggaggatg taactagtaa gtttgcaaat agttacccag
      121 aaaaagggtg gtatgatatt tattgggggc agtttattgc atcaaatgaa acgtatgtaa
      181 ttgttgtaga aacaccattt actaatgcag taactttgaa tactacttta tcagattata
      241 atgagaacaa tggtgtagaa cataatcata cttactcatc tgaatcggga tattcagatg
      301 taaatgctca agaaagaaaa attttatccg aattagtaag tagctcagaa tcagtatcaa
      361 gttcagaatc ggtatctaat tccgaatcta tttcaacttc tgaatcggta tctaactccg
      421 agtctatttc aagttccgaa tcggtatcaa gctcagaatc tatttcaact tctgaatcgg
      481 tatcaacttc agagtctatt tcaagctccg agtcagtatc aagttcagaa tcggtatcaa
      541 gctcagaatc tatttcaagt tccgaatcgg tatctaactc cgagtctatt tcaagctccg
      601 aatcggtatc taactccgaa tccatttcaa gttctgaatc ggtatcaagc tcagaatcta
      661 tttcgaattc agagtctatt tcaagctccg agtcagtatc aacttcagag tctatttcaa
      721 gctccgaatc ggtatctaac tccgaatcca tttcaagttc tgaatcggta tcaagctcag
      781 aatctatttc gaattcagag tctatttcaa gctccgagtc agtatcaact tcagagtcta
      841 tttcaaactc cgagtcggta tctagctccg agtcagtatc aacttcagag tctatttcaa
      901 gttccgaatc ggtatcaaac tccgaatcca tttcaacttc cgagtcagta tctacctcgg
      961 aatctatttc aagttcagaa tcggtatcaa gctcagaatc tatttcaagt tcagaatcgg
     1021 tatcaaactc cgagtctatt tcaaactccg agtcggtatc tagctccgag tcagtatcaa
     1081 attcagagtc tatttcaagt tccgaatcgg tatctaactc cgaatccatt tcaacttccg
     1141 agtcagtatc tacctcggaa tctatttcaa gttctgaatc ggtatcaaat tcagagtcta
     1201 tttcaagttc cgaatcggta tctaactccg agtctatttc aagttcagaa tcggtatcaa
     1261 attcagagtc tatttcaagt tccgaatcgg tatcaaattc agagtctatt tcaagttccg
     1321 agtcagtatc aagctcggaa tctatttcaa cttcagaatc ggtatctaac tccgagtcta
     1381 tttcaagttc cgaatcggta tctaattctg aatctatttc aagttccgaa tcggtatcaa
     1441 attcagagtc tatttcaagt tccgaatcgg tatcaaactc cgagtctatt tcaagttccg
     1501 agtcagtatc aagctcagaa tctatttcaa gttccgaatc ggtatctagc tccgagtcag
     1561 tatcaaattc agagtctatt tcaagttccg aatcggtatc taactccgag tctatttcaa
     1621 gttcagaatc ggtatcaact tcagagtcta tttcaagttc cgaatcggta tcaaattcag
     1681 agtctatttc aagttcagag tcagtatcaa gttcagagtc agtatcaagc tcggaatcta
     1741 tttcaacttc agaatcggta tctaactccg agtgtatttc aagttccaaa tcggtatcta
     1801 attctgaatc tatttcaagt tccgaatcgg tatcaaattc agagtctatt tcaagttccg
     1861 aatcggtatc aaactccgag tctatttcaa gttccgagtc agtatcaagc tcagaatcta
     1921 tttcaagttc agaatcggta tcaaactccg agtctatttt aagttccgaa tcagtatcaa
     1981 gctcagaatc tatttcaagt tcagaatcta tttcaagttc agagtcagta tcaatgagta
     2041 ctacagagtc tctaagtgaa tcagaagtat caggggattc tgaaattagc tcaagtacag
     2101 aatcatcaag tcaatctgaa tcgatgaatc atactgaaat taaatccgat tccgaatctc
     2161 aacacgaagt taagcatcaa gtattaccag aaacaggtga taactcggct tcagcattag
     2221 gtctgttagg tgcaggattg ttgttaggag ctactaaatc tcgcaagaag aaaaaagatt
     2281 aattttcaaa gtgatattta gatgataaat cgaaagtact ttcgatttat cacttaagat
     2341 agtaagtagt tatactgtct taagtgataa attgatttga aaagggaaaa aatgattgga
     2401 aaattatatt atagctatag aaagtcaacg ctattaagaa gtattttatg gactatttta
     2461 attgttggtg tatatatgtt aggacaacgt gttttattat ccactgttcc tttatcacat
     2521 caagagataa aactagcagt agatcaacat ttactcaata acttttcagc agtaagtggt
     2581 gggagtttta ataaattaaa tgttttcaca ctggggttga gtccatggat gtcaagtatg
     2641 attatttgga gattcgtttc cttattttcg tgggcaaaaa atgcaacgaa gcgaaaagca
     2701 gaagtagctc aatatacttt aatgcttact atctcagtta tacaagcata tggtgtttca
     2761 ggaaatcaat ttataaaaag ctctttatta ggttcttata gtgatattgt ttttaaagca
     2821 ttgacaatgg tcttgttaat aagtggctca tttgtgttaa tgtggcttgc taatctcaat
     2881 gcttctaaag ggctaggagg aagtattgtt atcatattag tgagcatgct ccaagcaacg
     2941 actactggaa taataactta ttttgaacag tcacatttta gtattatgga tctgctggga
     3001 actattatag ctataagtat aatacttagt gttttattat taatctcaac ggtggtttat
     3061 agggcagaat atcgtatacc tattaggcgt attgatatcg ttagtaaatt tgcccaagat
     3121 acctatattc ctattcgcat aaatccagca gggtcctacc ctttcatgta tgggatgaca
     3181 ttaatgatgc ttccaacgtt tataattcaa gcattgttac ttgcttttcc aaacgagcga
     3241 ttattgattc agatctcgtc aatgattgca attaataagc ctttaggtat tggacttttt
     3301 gttatattat tatttatttt gagcattgga tttgcatatt ttaattacga cccgtataat
     3361 attgcaaaaa atatgcgtca acggggggaa tattttcaaa atgtaactcc aggagctgct
     3421 actcaacgtt ttttacaaga aaaaattagt atcatggcag tagttggagc aatttttacg
     3481 gttattatgg gaggtctccc aatgttggct gtggtgggga agacaaacca agttagtatt
     3541 gcaatgatcg tcattaatat ttatattatt tcaactttta tgctcagtat tattgagcaa
     3601 gttaatacct tgaagttgtg gggcagtcac cgaaatatta tttaaggagc aatcatgttt
     3661 tattttattc cttcgtggta caattctgat caaaagtggc ataatgaagc tcctatctgg
     3721 tatcgtgtat tggagaacat gacttttgat gatactgtta gtcaggttag aatgtttcag
     3781 aatgcagatg aggaagtgcg tttacttcta tttagttata atcctcagtt gagatacatg
     3841 cttcatgaag agagtatgtt ggctgtctct tattggtctt tttttgacga tattcaaaat
     3901 attcaacaaa aagagattaa gttaattaat tttaaagaac tgaattggcc taaagggact
     3961 tattttgttt atacaccttt tttagtatta gctaaacatg gtggcgagat tattgcacgt
     4021 atccaatttg cggaaaatgg gaatttacta ctgattgaac ttttaataga tggtgtgctt
     4081 gataagatta tgatatttga cgatcgtggt tttgtatcca gtatcaaata tttcaaacaa
     4141 aatagattaa tttatcaaga ttatttaaat caaaatggtg tttggcaaat tcgagagtat
     4201 gcttcaaaat ttccgagaat agtagttaat ccaatgtctg ataaggtgtt taattttact
     4261 gagtatgatg attgggatac tcttattcga gagagaatat tggttttaaa aaaactagaa
     4321 gtgactccag atgattatat tatagtagca gtaaacgata ttcataacaa tttagtcaga
     4381 aaatcatttc cagatcaaaa gaaaattttc tcattttttg gtgaacgcta ttcatttgat
     4441 aatcgactcg actttaatga acttcttgat gaaggtgaat tgtttattgc tgaaagcgaa
     4501 aaaatagaac ggttattgat tacttcatta caagagaaaa attattcgtc aaactatgtt
     4561 gagaatcaca ttagccgtat tccccctttt gatactcgtt tgagactcgg acagagccaa
     4621 aatattgaag agcttattgc atatttactt gtagatgatc tttctcaaac ttatttaagt
     4681 gaagttttag accaattatt agcaaaaatg gaagttaatc cgtcattaga gttgaaaata
     4741 gtcagctata aaaatgatta tgatatgact tctctaaaag aacaagttaa ggacaaaata
     4801 ttgtcttcta aaaatccaga ttattttttt acctctcttt ctttggaaga tgagaatcat
     4861 tttaaggaag aacaaaaaac taaaattcgt ggcattagtt ttgatatata tacaaatgaa
     4921 aatcaaatta tagatgctct agatactgct aggttagtta ttgatttaag agatgatcca
     4981 gacctatata ctcaaattgc tgctctgagt tctggaattc ctcaaatcaa taaagttcac
     5041 actaattatg tagaacacca gaagaatggc tggattatta gtgactctac agaactcaat
     5101 aatgcaattg actattattt tgatggacta atgaattgga ataaatcgtt agtttacagc
     5161 gtaaaaaaaa tgggtgatta tacaagcggt aagatattac agcgctggcg aaaattatta
     5221 gaaaaagaat aatatggaaa aattaaaaat tttgcagatt ggtgaggaac catgggatgc
     5281 tgatttaata actccagaca atattgaatg gttatactgt aatggaaatg ctattcaaat
     5341 tttcttagag aaattaaaaa gtaaagagtt agaaaaaaat aatgttgaat atattggtaa
     5401 aacttcaact aatgaagtta aattatggtt taatgcggtc attttaacta gtgaggttag
     5461 cgaattgcaa ttgaacctac tttcagattg cttagaagcg catacactct tttacgacga
     5521 ggattttcaa ctcgattttt gctcagaaaa cggtatattt aaacgtaagg ttttgagacc
     5581 actaccattt aatggatcaa ggaaagaaaa aattattttc ctaagccgta ctcttttttc
     5641 ggaacaatat ggtgcaaaat tgaaggtttc tgacatagat ctaaatcctg attttaaggg
     5701 agagataata tatgaaggca atagctctat ttgtttttca gggaattttg gagaagattt
     5761 tcaacctcta ttctcatttc gctataattt atcctctact gataaagcga tagaaatatg
     5821 gcaagaatat caaaaattgc aaggtgataa tagtattatg atagaagtca ttcaatacca
     5881 aaaaggatca cttgatttga tattaaatac aaaatacctt tcagaaaatg atttggctga
     5941 tccatatatt ttagaatatg atgaatcagt tggtttttca tcaatttcta tctttgctag
     6001 aggtgacgga gtggtgagat ttggtgcact acattggcga tattcgcgta aaggtttggg
     6061 tcaattttta atcggtggag aaaggtacag tgattccaaa aggcaagagt ttatctacta
     6121 ttttaaccct ggagatatga aacctccatt gaatgtttat ttttcaggtt ttcgaggagc
     6181 cgaaggtttt gagggttttt acatgatgaa acgtttggga gctccgtttc tcttaattgg
     6241 tgatccaagg attgaaggtg gaagcttcta cctaggtact tcagaatacg aaagtgaatt
     6301 agaaaatgtt ttacaaaaat atttagatta cctacaattt gatcattgtc aacttatttt
     6361 atcaggttta tctatgggaa gttttggtgc cctttattat ggttctactt tttcccccca
     6421 tgctatagtt gtggggaaac cctttacaaa tttaggcgat atggtagtaa atttaaaatt
     6481 aagaagacca gatgattttg aaacgagtat ggatattctc caaaatatta ttggttctca
     6541 aaacgatgaa gctattaatc aatttaatca aaaattttgg aataagttta agcgttctaa
     6601 ttttgaaaaa acgcagtttg ctatatctta tatgaaacat gatgattatg atagaaatgc
     6661 aactccaagg ctgattgatt atttttctaa aactaacacc ctgttgtatg caagtggata
     6721 tgctgggcga cataatgata atagtcaatc aataaataat tggttta
//
LOCUS       DATRNX010000096         6747 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRNX010000096 DATRNX010000000
VERSION     DATRNX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            116..1399
                     /gene="aroA"
                     /locus_tag="VBC81_001615"
     CDS             116..1399
                     /gene="aroA"
                     /locus_tag="VBC81_001615"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6912642.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1392..1904
                     /locus_tag="VBC81_001616"
     CDS             1392..1904
                     /locus_tag="VBC81_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6912643.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1961..3334
                     /locus_tag="VBC81_001617"
     CDS             1961..3334
                     /locus_tag="VBC81_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6912644.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3435..4790
                     /gene="rlmD"
                     /locus_tag="VBC81_001618"
     CDS             3435..4790
                     /gene="rlmD"
                     /locus_tag="VBC81_001618"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6912645.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            4898..5119
                     /locus_tag="VBC81_001619"
     CDS             4898..5119
                     /locus_tag="VBC81_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912646.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            5237..5974
                     /gene="aphA"
                     /locus_tag="VBC81_001620"
     CDS             5237..5974
                     /gene="aphA"
                     /locus_tag="VBC81_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6912647.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            6294..>6747
                     /locus_tag="VBC81_001621"
     CDS             6294..>6747
                     /locus_tag="VBC81_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6912648.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHV"
BASE COUNT         2377 a          977 c         1324 g         2069 t
ORIGIN      
        1 agttaattta taaaagatgt tacagctata tagaggagct atagtataaa taaaaaattc
       61 aaaatatagc atatattatg atacaataat agcaaaatag attttgggag ttatcatgaa
      121 attactgact aatgcaaata ctctcaaggg aaccatccgt gttcctggcg ataaatcaat
      181 aagtcacagg gctattattt ttgggagtat atctcaggga gttacccgaa ttgttgatgt
      241 cttaagaggc gaggatgttt tatcaactat agaggctttt aaacaaatgg gggttctaat
      301 tgaagacgat ggcgagataa tcactatcta tggcaaagga tttgcaggtt taacacaacc
      361 gaataatctc ttagacatgg gaaactctgg tacttcaatg cgtctgatag caggtgtgtt
      421 agctggtcaa gagtttgaag tgacgatggt tggagacaat agtttgtcaa agagacctat
      481 ggatcgtata gcattgcctt taagcaaaat gggagctaga atttcgggag taactaaccg
      541 tgatttacca ccattaaaac ttcaaggtac aaaaaaatta aaacctattt tttaccattt
      601 acccgtagct tctgcacaag taaaatcagc tttaattttt gcggctttac aaactaaggg
      661 agaatctctc attgtagaaa aagagcaaac aaggaatcat actgaggaca tgattcggca
      721 atttggtgga catttggata ttaaagataa agaaattcga ctcaatggcg gtcagagctt
      781 agtaggacaa gatataagag ttcctggtga tatttcgagt gctgcctttt ggatagtagc
      841 gggtcttatt attcctaaca gtcatattat tttagaaaac gtaggtataa acgagacacg
      901 aactggaatc cttgatgtcg tcagtaaaat gggtggaaaa attaagttat cttcggttga
      961 taatcaagta aaatcagcaa cccttacagt tgattactct catctacaag ctactcatat
     1021 ttctggggct atgattccac gtctgattga tgaattacct attattgctc ttttagcaac
     1081 acaagctcaa ggcaccacag tcatagcaga tgctcaggaa ttaaaggtga aagagacaga
     1141 tcggattcag gtcgttgtcg aatcgctaaa acagatgggt gctgatatta ctgctacagc
     1201 agatggtatg attatacgtg gtaatacccc tcttcacgct gcttcacttg attgtcatgg
     1261 agatcataga ataggaatga tgatagctat tgctgctctc ttggtcaaag aaggcgaagt
     1321 agatttatct ggtgaagaag ctattaatac aagctatcct aactttttag aacatttgga
     1381 gggattagtt aatgcctaag gtattattgg gattcatggg agttggcaag actagtgttg
     1441 ccaactgctt agaaaatgaa gtaattgata tggatagtct gattgaaaaa catattggta
     1501 tgtcaatttc ccgatttttt acagaagaag gagaagcctc ctttcgtgct ttagaatctc
     1561 aatttttaaa cgaactttta aagaaaaaaa atgaaggact tgtgatagct agtggaggag
     1621 gtattgtact ccttgaagaa aatcgtcgtc ttttaacttt aaatcgtcat aataatattc
     1681 ttttaacagg aagttttgaa gtcttatatc atcgtataaa aaaagatgag aagaatagaa
     1741 gaccattatt tttaaaccat tcaaaagaag aattttatga tatttatcaa aaacgcatgt
     1801 tactctatag tggtttgtct gatatgatta ttgataccga ttatcttaca ccacaaaaaa
     1861 tagctactgt aattggagag tcactggtta atttatctaa ctaagttatc acagtttggt
     1921 agtatgaact attattattg aaagtaaatc acctgaaaat atgtcaagaa ataattacgg
     1981 tcagctaaat catcatgaag agctacgata taactatctt ttaaaaaaca tccattatct
     2041 taatgaacgt gaaaagatgg aatttcagta ccttcattat aaaaaaacag cagtacgacc
     2101 acagagaagg acagaaagtc caccaactaa ttcttattat gaggaacctt attctgattc
     2161 ttattatcaa gatgacgatt tttactcaga gcctcagcta acctcacaag gtttaccaat
     2221 ttatcaagaa gagagagctc cgaagaagaa aaaacaaagg gccagaaaag aaaaacagag
     2281 ggtgaaggtt atggcgcctt tccctccaaa ggctataacc ccccctagaa agaaaaaaaa
     2341 atttaagggt ttcctaaagt tcataggtat tatattacta attgttttat caggtatggt
     2401 tttcatgttt gttaaaggaa tgcgtgatgt gaataatggg aagtcacatt atagccctgc
     2461 aattattgaa gattttaagg gaaaagatgc tgttgatggg actaatatct taattttagg
     2521 tagtgataaa cgtgtcagcg agagatctac tgatgcgaga acagatacca taatggttgc
     2581 taatgttggt aacaaggata ataaggttaa gatggtgagt tttatgcgag atctcttagt
     2641 caatattcct aattatagta cggaaggtta ctatgatatg aaattaaatg cgtcttttaa
     2701 tttgggagaa caagataatc ataaaggagc agagtatgtt cgacaaacgt tgaaaaatca
     2761 ttttgatatt gatataaaat attatgttat ggttgacttt gagacatttg cagatgctat
     2821 tgatacactg tttcctaatg gtgtcaagat taatgctaaa tttggacttg tcggtggaca
     2881 atctgctgat tcagttaaag ttcctgatga tttaaggatg aaaaatggcg ttgtacctag
     2941 ccaaaaaatt aaggttggga ttcagtacat ggatggaagg acccttctaa attatgctcg
     3001 tttcagaaaa gacgatgatg gggactttgg tcgaacacaa cgtcaacaac aagttatgag
     3061 agctattgtc tctcaaataa aagacccacg tcgtctcttt acaggttctg ctgctatcgg
     3121 taaagcctat gctttaactt caagtaattt atcttatagc tttgtcctaa cagatggcat
     3181 tccaatttta tcagatgcta aaaatggtat taagcaaatg acaattccaa gagaaggaga
     3241 ttgggtagat gattatgacc agtatggtgg tcaagggtta acaattgatt ttgctaagta
     3301 caaaaagatc ctaaagaaaa tgggactgcg ttaacatttt taattatgat ataatagagt
     3361 gatttattgt cactctattt ttatctttag tgtataaact aaagacttca aatgatagat
     3421 ataaaaggaa aattatgtta cataaaaatg atattattga aactgaaatt agcgacatta
     3481 gccatgaggg aatgggtatt gccaaagtag atggctttgt tttctttgtt gaaaatgctc
     3541 ttccaggtga gattattaag atgcgcgtct tgaaattacg taagcgtata ggatatggaa
     3601 aagttgagga atatttaacg acttctcctc accgaaatga aggattagac tatacctatt
     3661 tacgtacagg gattgctgat ttgggacatc ttacttatga acaacagctg ttatttaaac
     3721 aaaaacaagt agctgataac ctttataaaa ttgctcatat atctgatgtt ttagtagaac
     3781 ctactcttgg tatgacaatc cctttggcat atcgaaataa agctcaggta ccagttcgtc
     3841 gtgttgatgg acagctagag acaggttttt tcagaaaaaa ttcacatact ctggtatcca
     3901 ttgaagatta tttaattcag gaaaaagaga ttgatgctct gattaatttt acacgagatt
     3961 tattaagaaa atttgatgtt aaaccatatg acgaagagca gcaatctggt ttaattagaa
     4021 atctcgttgt tcgtcgagga cattatacag ggcaattaat gttggttttg gtgacaacac
     4081 gtcctaaaat attccgcatt gaccaaatga ttgaaaagtt ggtgtctgcc tttccttctg
     4141 ttgtttcaat tatgcaaaat attaatgatc gtaatagcaa cgttattttt gggaaagaat
     4201 ttcgtactct atatggttct gatactattg aggaccaaat gttaggaaat acttacgcta
     4261 tatcagccca atctttttac caagttaata ctgaaatggc tgaaaaactg taccaaaaag
     4321 caatcgattt ttcagattta aactcagagg atatcgttat tgatgcctat tcaggtatag
     4381 gaacaattgg cttatctgtt gctaaacaag taaaacatgt ttatggtgtt gaagttgttg
     4441 aaaaagcagt aagtgatgcc aaagaaaatg ctactcgaaa tgggataact aacagtacct
     4501 atgttgctga ttcggctgaa aatgcaatgg caaagtggct taaagaaggt ataaaaccaa
     4561 ctgttataat ggttgatcca ccacgaaaag gattaacgga gagttttgta tattctgctg
     4621 cccaaacaaa agcagataaa atcacttata tttcttgtaa ttcagcgaca atggcgcgtg
     4681 atatcaaact atttgaagaa ttagggtatc atttagtaaa aattcaacca gtagacttat
     4741 tcccaatgac acatcatgtg gagtgtgtgg ctctgctcgt aaaagcctag aaacttttta
     4801 acgaaaggaa tattaaaaag ccttagataa tcgactgacc tattaccaaa aaatcgaagg
     4861 ggctctactt gccattgcac aaacaccagg aaatagtttg ccaactatgc agctgttaga
     4921 tgagttagtt cttccaaaag acattaccat agcagatgtc agtcaaagta tccaagaatt
     4981 tgaaatagaa agagatgccc ttcaagaatt ttttgacgat atgctgaaga cttatacaac
     5041 ctaccaagaa atgaaagatt atattaaatc tcgtgaagcg aacactccta agcaagagac
     5101 agaagaaaaa tcactttgaa gactgtagac ttaacataag taaaaaataa ccatatatga
     5161 tatcgctttc attcttatgt tttttgtgat aaactaatta aagaaataat ttttatactt
     5221 tattatggag gttcttatga agaaagtgtt agtgagtagt cttttggttt tagggattac
     5281 gataacgtta caaacagtag ttgaggctaa ggggccaaaa gtagcttata cacaagaggg
     5341 aatgactgct ctttcggaca caaataaaga taaagtcact actatttcta ttgacgagat
     5401 tcaaaaaagc ttagaaggta agaagccgat tactgttagt tttgatattg atgatacact
     5461 gcttttcagt agtcaatatt ttcaatatgg taaagaatat gtaactcctg gatcgtttga
     5521 ttttcttcat aaacaaaaat tctgggatct tgttgcaaaa cgaggagatc aagattccat
     5581 tcccaaagaa tatgctaaaa aattaattgc tatgcatcaa aaacgaggag ataaaattgt
     5641 ttttataaca ggtaggacaa gagggtcaat gtataaggag ggcgaggttg ataaaacagc
     5701 taaagcctta gctaaagatt ttaaattaga caaaccaatt gctgtaaatt atacaggcga
     5761 taaacctaaa aagccataca aatatgataa atcatattat attaagaaat atggttcaga
     5821 cattcattat ggagatagtg atgacgatat tcatgcagct agggaggccg gtgctagacc
     5881 aattagaatt ttaagagcac ctaattctac aaatctacct ttaccagaag ctggaggcta
     5941 cggtgaagag gttctcgaaa attcagctta ctagtatctt tattagttaa catagtatta
     6001 tctatcaatt tttagatttg taataagaag tgctaaaaaa gtagcaatca atgatatgct
     6061 aataagggaa gttggagtag tttagattga ttattaatga ctgatcttct ctcctctcgg
     6121 taacaagaag tttttctaac aaatgcctgt ctaaaaaaat tgaatcataa ttttttaata
     6181 actcttttgg cgtgaacaag gaatttgtaa tcagtcatct aattctataa tacaatactt
     6241 tggggttgtt tccgtatcat tattaataat atgtatcaga aaggagatgt tttttgagag
     6301 tttatgaaaa taaagaagag ttgaaaaaag aaataagtaa aacatttgag aaatacatta
     6361 tggaatttaa taatattcca gagaatctaa aagataaaag aattgatgaa gttgatagaa
     6421 ctccagcaga aaacctttct tatcaggttg gctggaccaa cttggttctt aaatgggaag
     6481 aagatgaaag aaagggactt caagtaaaaa caccatcgga taaatttaaa tggaatcaac
     6541 ttggtgaatt atatcagtgg ttcacagata cctacgctca tttatctctg caagagttga
     6601 aagcaaaatt aaatgaaaat attaattcta tctatgcaat gattgatttg ttgagtgagg
     6661 aagaattatt tgaagcgcat atgagaaagt gggctgatga agcgactaaa acagcgactt
     6721 gggaagtgta taagtttatt catgtaa
//
LOCUS       DATRNX010000097         6710 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRNX010000097 DATRNX010000000
VERSION     DATRNX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(103..1263)
                     /locus_tag="VBC81_001622"
     CDS             complement(103..1263)
                     /locus_tag="VBC81_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912649.1"
                     /translation="MIKFTYSANFFYYNCSKRIKEKMDSINQLIDEYGYTSSRLAANR
                     NTARKPKISRNAITLDYTKEFSRALNNKITQRNPNFITPALTEDILNFCKPEEDFIRQ
                     LNIDGIDSCKYEFESVSEIYWGTDEELYKNEELFFYAIIKDMLNSIDDKNFIESILID
                     YVPYAYLTALNEIDSSLPPNAVSIFDYYPDLPNLKETQKTLSEAISRLYQQFKNQYSL
                     TDMFFDFLSKNKKEVEFKNMQTEFKNFVNMYLKPRLKHMVTDENSLGKRVYELFKVDG
                     GIYITSQIFQTTISQPNKDLLDATIDYCNTLRNIQLEQENGLQSLSVELENYTQSIAL
                     SILALDKYPTGVTTIDKLLEKFESHSGGDLSAFKRIEYLEDESENDGQIYKY"
     gene            2044..2469
                     /locus_tag="VBC81_001623"
     CDS             2044..2469
                     /locus_tag="VBC81_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912650.1"
                     /translation="MKKINLNSTKLGELRFSVNEIEAPQVAIVTNARRVAKRDGNGEV
                     IDGTIAKLTILVLDIKLYQLMNQQGLDVSSVVPITVEVVGDENYLKQIIVEDIIGKVI
                     DLRSAYVGLKWVSRGNSGNWGGLKLILEELKFVNLNKDN"
     gene            2544..3950
                     /locus_tag="VBC81_001624"
     CDS             2544..3950
                     /locus_tag="VBC81_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6912651.1"
                     /translation="MMKTIFKEIISTYSLKNKKVATIIVSRTRKQRCQLLTNILLSIL
                     SVEMMFGLLYLLFDFVNSNQISISIIFSLVGLVCLESIMVLNWLFLNSKFSPIEKWKV
                     SALLKLFPEENDILRKHKSEIDTTQSVRWEYWIDNGLIFLKLYHGGHVPKGKLEEIPT
                     NILSFFIKETRDEWFLEDKNLADGYVKVVLSHKPDKSLYIKGREDFIESKTLKIRLTE
                     RLNWTLSQTNALIVGPSNSGKTTLLKYLILAFLINNKKNEVYTIDGKSAYLSNAMRHI
                     GEVATTGQQAFDMISKVEAIMEERYAEINQNSDDEADVTHNEKFGQGNILLVIDEYLA
                     LATEMQAEDKLKKPADRLFPQFNAKLLKLIVKCRQASIMVVVSGQLLPTSILSSESRD
                     SLGLRIALGRISQSQSTEIFNIGKSELPSVDSSKREGVIYLDGIGLEHPITFKTPFYD
                     DSRLPFKGALRVLKSERS"
     gene            4052..4921
                     /locus_tag="VBC81_001625"
     CDS             4052..4921
                     /locus_tag="VBC81_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912652.1"
                     /translation="MTSEYNLKVINFGDHVEVIKYGYLKQRVSGNNRPTNHERQIRTE
                     EEQEIYNFQQAQKIQRRIRYYCECNDFDLFWTLTFDDEKVNAKDYEYAKKKLRAWLKY
                     QREKYGKFDYIFIPELHKNGRIHFHGITFGFSPPLIEARNPKTDKLIKKNGLQIYNAE
                     TWKDGFSTVSKIDSKAKTANYISKYITKDLIAIPSAYRQPKYFVSRGLKKPEIEFQQI
                     DENELQEFTPNFIVGNLYIEEQKFEKDVSIYLLELDDENKFFQKKKPETVVRSRLKSK
                     NDENIENVSDENE"
     gene            4941..5198
                     /locus_tag="VBC81_001626"
     CDS             4941..5198
                     /locus_tag="VBC81_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO6912653.1"
                     /translation="MTNTKVTVKHIDLMKIGFPENTAREIIKQAKAIAVQRFENNIQE
                     TGKVIQLGQSPFDNVRLNLAPASIVEELLGFELPLLEKEGA"
     gene            5201..6433
                     /locus_tag="VBC81_001627"
     CDS             5201..6433
                     /locus_tag="VBC81_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912654.1"
                     /translation="MASKKYSGVSRDKKNRIYFQTEFGKDPVTGKRRRKKSYKDKNGN
                     PFRTEKQAYDELCRVRAEVRSQDELAQKQTVPVLTLEDFINTVFLPYYIKTVESSTYK
                     TALTHFDIFKEELGQYLLSDISVQFCERFRLSLQDKYSPNYAKNVWLRFKKCLTYAER
                     LEYIEFNPCTKLDNIKGERTVTSFWTVEDFQKAVNAFDRTTYQGVYYYTVIWLYFMTG
                     LRVSEGLSLIWSDIDFESKLLHVQSTLEYDENGNPFRKNKTKTEAGTRYVELDDMTLQ
                     TLKEWKEVQIHNSLGDFVLAREEVPMHKTTLTRLLKRVAKEQDLPVMAGKGLRHSHDS
                     FLINVLHKDVLYVSARSGRTDKATTLNTYAHIYAKKKAEGGREITKALESFGFDNPTK
                     TPPSKEEIAETLEITRFN"
BASE COUNT         2433 a          942 c         1214 g         2121 t
ORIGIN      
        1 taatattcat gaactaaaaa atgcattttc tcagattaaa ccgaaatagg ttttaataaa
       61 aaggcttcaa ctatcattta gtagttgaag ccttttttgc tattaatatt tataaatttg
      121 accgtcattt tctgattcat cttcaaggta ttctattctc ttaaaagctg aaaggtctcc
      181 cccactatga gattcaaatt tttcaagtaa tttatcgatg gtagtgacac ctgtaggata
      241 cttatctaaa gctaagattg ataaggcaat tgattgggta taattttcta actcaaccga
      301 gagactttga agaccatttt cttgttccag ttgtatgttt cttagagtat tacaataatc
      361 aatagtagca tctagaagat ctttgtttgg ttgtgaaatt gtggtttgaa agatttgact
      421 agtaatgtat attccaccat ctactttaaa taactcatag actcttttac cgagagaatt
      481 ttcatcggtt accatgtgtt ttaagcgagg ctttaaatac atatttacga aatttttaaa
      541 ttcggtctgc atatttttaa actcaacttc ttttttattt ttagaaagaa aatcaaaaaa
      601 catatctgta agtgagtatt gatttttaaa ctgttgatat agtcttgaaa ttgcttcgga
      661 gagagttttt tgcgtctctt ttaaattagg caaatcagga taataatcaa aaattgaaac
      721 agcatttggg ggcaaacttg aatcgatttc atttagtgct gttaagtaag cataaggaac
      781 atagtcaatc agtatacttt caataaagtt tttatcgtca atggaattta gcatatcctt
      841 gatgattgca tagaaaaata attcttcatt cttatagagt tcttcatctg ttccccaata
      901 aatttctgaa acagattcaa attcatattt acaagagtca ataccatcta tatttagttg
      961 tctaataaaa tcctcttcag gtttacagaa atttaatata tcttctgtaa gtgctggtgt
     1021 gataaaatta ggattccttt gggtgatttt attatttaaa gctcgagaga actcttttgt
     1081 atagtcaagt gtaatagcat ttctactaat ttttggtttt ctagctgtat ttctattagc
     1141 agctagacga ctagaagtat aaccatattc atcaattaat tggttaattg agtccatttt
     1201 ttcctttatt ctctttgaac aattgtaata aaagaaattg gcactatatg taaatttaat
     1261 cataaaaagc tcctaaaaaa ttttgtactt ctattataac ctaataatgt tctttgctaa
     1321 ggggagaatt tggacttctt attctctatt ttttcccctt agcaaagaat ttgcttcctt
     1381 gtataatgag aatagagctg acagccactc ccaatcaaat gtttttgggg gcgaaaatag
     1441 tgaacaaaag tttgaaaact ccccttagca aagttctctc tactttatat aataagggta
     1501 gagctggcag tcagtcccaa ccaaatattg ggggcgaaaa tagtgaacta aaagtttgaa
     1561 aactcccctt agcaaagttc tctctacttt atataataag ggtagagttg gcagtcagtc
     1621 ccaaccaaat attgggggcg aaaatagtaa actaaaagtt tgaaaactcc ccttagcaaa
     1681 gttctctcta ctctatataa taagggtaga gttggcagtc agtcccaacc aaatattggg
     1741 ggcgaaaata gtaaactaaa agtttgaaaa ttccccttag caaagttctc tctactctat
     1801 ataataaggg tagagttggc agtcagtccc aaccaaatat tgggggcgaa aatagtaaac
     1861 taaaagtttg aaaactcccc ctaccaaagt tctctgttct ctatatatta aggttaatat
     1921 taggtagcta gttctcaaat taaagtaaca gaggaacgac catagaattt tatagctata
     1981 aagtctaatc aatgcttgtc ggcttagtta agacataaat aatttatata gaggtaaata
     2041 acattgaaaa aaatcaattt aaatagtaca aaacttggag aacttcgttt ttcagtaaat
     2101 gaaattgaag ctcctcaagt agcaatagtc accaatgcta gaagagtagc aaaaagagac
     2161 ggcaatggtg aagttattga tggaacaatc gcaaagctta cgattttagt tttagatatt
     2221 aagctatatc aacttatgaa tcaacaagga ttagatgttt caagtgttgt acctattaca
     2281 gtagaagtcg taggtgatga aaattatcta aaacagatta ttgttgaaga tataattgga
     2341 aaagttattg atttacgttc agcctatgta ggtttgaaat gggtttctag aggcaattca
     2401 ggaaactggg gtggattaaa attaatttta gaagaattga aatttgttaa tttaaataaa
     2461 gataactaaa aattaaatag gaatattagg gatagggggt agcaatgcta catgggtata
     2521 tatttttaac aaggaaaaaa ttaatgatga aaacaatatt taaagaaata ataagtactt
     2581 actcactaaa aaataagaaa gtagcaacta tcatagtgag caggacacga aaacaacgct
     2641 gtcagctatt gactaatatt ttgttgtcta tcttatcagt tgagatgatg tttggcttac
     2701 tatacctgct ctttgatttt gtgaactcta accaaatttc aatatcaatc atatttagct
     2761 tagtaggctt agtttgcttg gaaagtatta tggttctgaa ctggttattc ttaaactcta
     2821 agttttctcc tattgaaaaa tggaaggtgt cagccttatt aaagctcttt cctgaagaaa
     2881 atgatatttt gagaaaacat aaatcagaaa ttgatacaac acaaagcgtt agatgggaat
     2941 attggataga taatggcttg atatttttga aattatatca cggtggtcat gttccaaaag
     3001 gcaaattgga ggaaatccca actaatattc tatcattttt cattaaagaa acacgtgatg
     3061 aatggttttt agaagataag aacctagcag atggatatgt gaaggttgtc ttgtctcaca
     3121 aaccagataa atcactttat ataaaagggc gtgaagattt tatagaatca aagacattaa
     3181 agattcgatt gactgaaaga ctgaattgga cattatcaca aactaatgct cttattgttg
     3241 gaccgtcaaa ctctggtaaa acaaccttac tgaaatatct aattctagca ttcttgataa
     3301 ataataagaa aaatgaagtt tatacgattg atggtaaatc agcctatcta agtaatgcga
     3361 tgcgacacat tggtgaagta gcaactacag ggcaacaggc gtttgatatg atttctaaag
     3421 ttgaagctat tatggaagaa cgttatgctg aaatcaatca aaattctgat gatgaagcag
     3481 atgtgacaca taatgaaaaa tttgggcaag gaaatatttt gctagttatt gatgagtatt
     3541 tagctctagc tacagaaatg caagcagagg ataaattaaa gaaacctgct gatagactgt
     3601 ttccacaatt taatgccaaa cttttgaaat taattgttaa atgtagacaa gcttctatta
     3661 tggttgttgt ttcgggtcag ctgttgccta cgtctatttt gagctctgag agtagagatt
     3721 cacttggttt acgtattgct ttgggtcgta ttagtcaatc tcaatcaaca gaaattttta
     3781 atataggaaa atcagaattg cccagtgtgg actcaagtaa acgagaaggt gttatctact
     3841 tagatggtat tggattagag cacccaatta ctttcaagac accattttat gatgattcta
     3901 ggctaccatt caagggggcg ttacgtgttc tgaaatcaga gcggagctag ctccgctctg
     3961 attctaggaa taaataagga tacttttggc tggctacttg acagacagcc aagataagct
     4021 gagggtatga tagaaagttg gtaaattatg agtgactagt gaatacaatt taaaagttat
     4081 aaattttgga gaccatgttg aggtaataaa atatggttat ctaaaacaaa gagtatcagg
     4141 taataataga cctaccaacc atgaaagaca aatacgtaca gaagaggaac aagagatata
     4201 taattttcag caagctcaaa aaattcaacg aagaattaga tattactgtg agtgtaacga
     4261 ttttgattta ttctggacat taacttttga tgatgaaaaa gttaatgcaa aagattatga
     4321 atatgcgaaa aagaaattaa gagcttggtt gaaatatcag cgtgaaaaat atggaaaatt
     4381 tgactatatt tttataccag aattacataa gaatggtaga atccattttc acggaataac
     4441 ttttggattc tcaccaccat taatagaagc aaggaaccca aaaacggata aattgataaa
     4501 aaagaatggt ttgcaaatat ataatgctga aacttggaaa gatggtttca gtaccgtatc
     4561 aaaaattgat agcaaggcaa aaacagcaaa ttatatatcg aagtatatta caaaagattt
     4621 gattgctatt ccttcggcgt atagacagcc aaaatatttt gttagtcgtg gtttgaaaaa
     4681 acctgaaata gaatttcagc agattgatga aaatgaactt caagagttta cccctaattt
     4741 catagtaggt aacttatata ttgaagaaca aaaatttgaa aaagatgtct caatttacct
     4801 gttagaatta gatgatgaga ataaattttt tcaaaagaaa aaacctgaaa cagtcgtaag
     4861 aagtagattg aaaagtaaga atgatgagaa tattgagaat gtttcagatg agaatgaata
     4921 agaaaattag gattattaat atgacaaata caaaagtaac agtaaaacat attgacttaa
     4981 tgaaaatcgg attcccagag aatacagcta gagaaattat aaagcaagca aaagcgattg
     5041 cagtacagag atttgaaaat aatatacagg aaacaggaaa agtgatacaa ttaggacaat
     5101 caccatttga taatgtgaga ttaaacctag ccccagctag tatagttgag gaattgcttg
     5161 gctttgagtt gcctttacta gaaaaggaag gtgcttaaag atggcaagta aaaaatatag
     5221 cggagtatcc agagataaga aaaatagaat ttactttcaa acagaatttg ggaaagaccc
     5281 tgtaacggga aaacgaagac gtaagaagtc ctataaggat aaaaatggaa atccattccg
     5341 tactgaaaaa caggcttatg atgaactttg tcgagttaga gccgaagtca ggtcacaaga
     5401 tgaacttgca caaaaacaga cagttccagt attaacgtta gaagatttta ttaacacggt
     5461 atttttaccg tattatatca aaactgttga atcgtctacc tataaaacag cattgacaca
     5521 ctttgatatt tttaaagaag aactagggca gtacttactt tcagatatat ctgttcagtt
     5581 ttgtgaacgg ttccgtcttt cattacaaga taagtattca ccaaactatg caaaaaatgt
     5641 ttggcttaga tttaaaaaat gcttaactta cgcagagcgt ttggaatata ttgaatttaa
     5701 cccttgtact aaactagaca atatcaaagg tgagagaaca gtgacttcat tttggacggt
     5761 agaagatttt cagaaagcag tcaatgcatt tgatagaaca acatatcaag gtgtttatta
     5821 ctatacagta atctggctat attttatgac aggattacga gtttctgaag gattgagcct
     5881 aatttggtct gatattgatt ttgaatctaa attattgcac gtgcaatcaa ctttagaata
     5941 tgatgaaaat gggaatccat ttcgtaaaaa caagactaag accgaagcag gtacgagata
     6001 tgttgaatta gatgatatga cacttcaaac gctaaaagag tggaaagaag tacagattca
     6061 taatagttta ggtgattttg ttttagctcg tgaagaagtt cctatgcata aaactaccct
     6121 aactcgtctt ttgaaacgtg tagcaaaaga gcaagattta ccagttatgg ctggtaaagg
     6181 tcttagacat tcgcatgata gctttttgat taatgttctt cacaaagatg ttttatatgt
     6241 atcagcccgt tctggaagaa cagataaagc tacaacactc aatacatatg cccatattta
     6301 cgctaagaaa aaagctgaag gtggtagaga aataacgaag gctttagaat cgtttggatt
     6361 tgataacccc accaagaccc caccaagcaa agaagagata gcggaaacac ttgaaataac
     6421 aaggtttaat tgaaaaacgc aactgttact cttaaataat catctattgg ttgtttaaag
     6481 gtgaaagcct agttttaaag tcttttcgtt aaagttaagg cgattaaaaa aggaaagatt
     6541 caattgaatc ttccccacaa acacccccac aatattttgt gggggtgttt tgtttaatat
     6601 ggatttagaa tatcattata aagtatttct tgaataatta aacgattttc taattgaagt
     6661 tttgtgaata aatttctgcc atgattctta ttattaaaag tatctccata
//
LOCUS       DATRNX010000098         6658 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRNX010000098 DATRNX010000000
VERSION     DATRNX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1110)
                     /locus_tag="VBC81_001628"
     CDS             complement(<1..1110)
                     /locus_tag="VBC81_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6912655.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLI"
     gene            complement(1171..1440)
                     /locus_tag="VBC81_001629"
     CDS             complement(1171..1440)
                     /locus_tag="VBC81_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912656.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            complement(2785..2961)
                     /locus_tag="VBC81_001630"
     CDS             complement(2785..2961)
                     /locus_tag="VBC81_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912657.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            complement(4059..4664)
                     /locus_tag="VBC81_001631"
     CDS             complement(4059..4664)
                     /locus_tag="VBC81_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6912658.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            complement(4913..5703)
                     /locus_tag="VBC81_001632"
                     /pseudo
     CDS             complement(4913..5703)
                     /locus_tag="VBC81_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(5757..>6658)
                     /locus_tag="VBC81_001633"
     CDS             complement(5757..>6658)
                     /locus_tag="VBC81_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEO6912659.1"
                     /translation="DGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGNDGSQLKNGVALG
                     GPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDNYISNKFYNTNN
                     RTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIKVNKDKHSESLL
                     GAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYEISSPDGYIEVK
                     TKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPPGVFPKTGGIGT
                     IVYILVGSTFMILTICSFRRKQL"
BASE COUNT         2118 a         1313 c         1007 g         2220 t
ORIGIN      
        1 tattaaaagc attaaaaaga aagaaaatac aaagtaaaag actaagtgga aaatcttatt
       61 aaggaacata aatttttgat aatattcaaa tgcttttggt gccacgtgac tagcatcact
      121 atagactttg ttattcataa aaagcaccat tgtatcatgc gtctttagat ctgcataacc
      181 aaaacttgaa tactggaaaa aggccccatt agaattaact atacgaccat gcatgtaaag
      241 cccaccataa taatgtgata tcgcatcatt ttgatgtttg gtatattcat ccgttgggaa
      301 aaagtaccct ttagaaaaag accgcatcaa cacccaaaag ttcagcggag ttgtgaaaaa
      361 atctccagct ccatatgctg tggatacttg attaaagata ttaattttat ttaacttgcc
      421 gttctttcgc attgggctaa ctaagtcatg attaacaact gattggtaac tttgggtatc
      481 atctaatcgc aaaggatttt ttatcacatc atcaacaagt tcagcgtagg tccttcccgt
      541 aacttgagtg gctatacctg ttaggaaact aaagttgata ttagaataat accacgattt
      601 cttattaaca aagttatagt gttttaagca aaattgtaat tgttctctct cattttttag
      661 ataaggagac accttaacac ctttttctct taacccactc gtatgggtca acaattggtg
      721 gatggtaata ctattactac cttttacttg gggaaagaac tgagacaatc ggtcgtcttc
      781 actcaagaca tcttgatcaa ttaatctttg gataataatt cctgtcatga gtttttggag
      841 agaagccatt ggaaataatt tattattttc aatatccgtt tcaacttccc cacggttaat
      901 cccatgcgag atggtaatcg gtttaccagt attatcgcta acaatcatca ttccattaac
      961 attatttttt cgcataaagc gatgcaataa aatcggtgta atattccctt tattttctgg
     1021 aataaaaaag aaacatgcca ctgaaatacc ggtggcgatt actgctataa gaaagagaga
     1081 taggagcgat tttaaaaatt tacgtaccat aaataacctc cttaatatca taacacaggg
     1141 ctaaattatt tcaaagacat tttatatatt ctagcaaaaa atatgttcgt cgctaatatt
     1201 attttcaact cctatcattg ctacctcaat atttcctttt agattctcac tatgatcttg
     1261 atagttataa ctcttctgat aagtacggat tcctaaaaaa acttggtaat tctcttcagt
     1321 cactttagcc ttctcaaaat tttctgtttc aagataagaa gatatcgctt ctctataaaa
     1381 aacattatga aactctatca actcctcctg ctttactttc aagagaagca agctcaccat
     1441 ttctttaccc tgtttaaaac actttctcta gtgtcactcc aatcttttaa actgttttta
     1501 ggacattttt taaagacaga gataaggtta tcaatgtcct tgaatcgcct tattcaaaca
     1561 ccaaacttga gcaaccaata atctcatcaa aatcatcaag cacaaacatt tagctttaga
     1621 aactttgaat attttaaaaa gagaattcat atcactttga atatcaaaaa ggagaaaacc
     1681 aatttggttt tctccagaat ataatttttc atcaacccac tacagttgac aaagagccaa
     1741 aaaacaagga atcaatcaag aaattttgat tgattcctcg ttttatgctt atatgagata
     1801 ctttaaacaa attttttcaa tgtgatccaa ctatctagtg actcctcgat atccttttat
     1861 atctctcacg atagctttga ggagtaagta ctattccatt cgttttacct ataatttcac
     1921 caaatgcata cataccatat cctatataac ggctatcgtt ttaagaacaa tttatttatg
     1981 ttggcagaaa ataataatag gaagcagaaa aatccaataa tattcaagag aaaaagtata
     2041 ggtgatagct ctctaaaaaa atttgatatt gaaactataa gaaaccctgt agatacgata
     2101 gttgaaacaa cctttttctt atccatatta ataaccaccc cttaaaacaa tttcatatcc
     2161 tatatacttt gtacgcataa cgaatccctc ctaaattatt tttttgtctg ttgcaaccct
     2221 gacaagttag tgtagcacga aaaaacgatt atattctcaa aatcttatct tctagctgct
     2281 caacttcttc ttcaatacga gggtcttcat cattcttagt gttaaataaa gcgagattgg
     2341 atcaaccagt gtcacgttag cctctccaag ctatttattt ttctagtaat caaagggatt
     2401 ggttcataat tccaaatctc tagccgatga ctttgaagga tgtatagttg aagacttgat
     2461 tcgaaacttt tgtatctttt ccttgaaata gtatagtctc cgtatttttc cgaatcatca
     2521 agcaagccta gatatgataa gtctttaaaa taagtcggtt aaaataatag ctcttgtttt
     2581 aatttttgaa aatccttatc acttataatc aacgcttctc taactggact atctattaga
     2641 tctttggact cttcaaatat catatttttt ggcttcgcaa attggtaaga tttctgttta
     2701 ttatgaagcc atccacgctc tttaaaattt ctaaagcaac aatataagtt ttagaataga
     2761 aagatcggtt agctgactta cttcttacat atcaatgatt aaacctttag tatgtaataa
     2821 taaggtaatc aataactttt gttcagtcgg agacaaccga ttatcaagtt caattttttt
     2881 agttaattta gaatatagaa aacctaattg tgggataaga gcattttctt gatagtcaaa
     2941 ataagacttc ccaacatgca ttaacttctc cttctgctca tcagatagct tattaaaaac
     3001 tagagtaatg cccatttcaa actattttta gatagcttga gcttgtttga caaagttatc
     3061 taaacgacct gacctttttt ctttaaccaa agggaggtgg aatcctaaca gttaagattg
     3121 aaaattcata tcgtcgacga aaaacaatta gaagttctag aactattagc tatatttgaa
     3181 tttcaaaatg gaaagcactt aggatactct tgatttttta aatatccata tttagctcct
     3241 actatcatta tatttgcatc ttatcaaaca taatgataat atgcaaactt ttcgataaaa
     3301 tggacaaata cgataataaa tatttcattt gttagtatat cctcacttta cttggtcata
     3361 taagcaaaat tcatggtact atctgtagat gtaatgtgac gaaagatata gttccgtagc
     3421 ctatatacga accataacta ctttcctaaa aagagagaat agtaccttct tttggaactg
     3481 tatcagtttc accatctagt ctttcggctg ttccaaccca atcctgacca tttcccattg
     3541 aactggtaat ggtaatcttc tcaccatttc aacctttgag tttcagtccc actgattaat
     3601 tcgtgccgca actccccaag tacatttgct ataagcataa cccatgacat catcaccgcc
     3661 cgaaatcgaa tcattacgga cactttctag tgcctttgga tcattctggg cggttcccct
     3721 tttggttgac taaagctacg atcgacttgg tagtaccacc ccgttgcgct ggtttggtgt
     3781 tcaatgagct tgtccccaga attcccctcc caatggatga gaactcgtga atcctgtttt
     3841 tccatatata tttttcctcc gtaaactaaa aaaagagaac actctaaaaa tgttctcgta
     3901 atgccagaaa tttatctgac gtatctattt ttttgtctac gcaagccact tcaaaacaaa
     3961 cggtcacgtc aatatgtttt ttaatacctt ttttatgtta aaatcactct tttcaataaa
     4021 gacaaaatgc ttgaaaaagt gcttaaaata aaggattttt accctttgct tcgttgtagc
     4081 aacgctacgc actccacatg gctggtatta tcagccattg ggtaatcagc ctctttgaag
     4141 ttagcttcat ccacaattgt cgtttttcct gaattttgaa cgaccacaac ataagtctta
     4201 ggatcaagta cgtaagcatt cggcgctttc gtttctttaa ggtcataaaa tccaggttta
     4261 aggttgtcaa aggtaagggt tgtccgaccg actgtcgtct ctttgctgac aggaatgaaa
     4321 tctccttgta cttgatgatc tgccgctact gttgtgcgtt tagacaaggt gaaggtcgca
     4381 cctactaagc cttctttagc ttcgtcaatc ttgttaatgc tgaacttcct gctcttttta
     4441 tccgtaaccg tcaaaatcga ttccggaatt tgtgacttaa tcgttgtccc aatagcttga
     4501 atctgagtgg tgtagtttgc attaataggt ggtgtaaata aagtgctgga gttgttagac
     4561 caagctacct taatattatc tgctggaaaa aaggcgccaa aagaggatga tagaactttt
     4621 ggatctaaat atctcgatct atcactatat ggatcgcgcg ccactaaaag ataaggaata
     4681 actcctaatg gcttcatgat atcaaagcta gactgacttt gaggtataaa agttttctca
     4741 gatgagctcc gctattataa cgacctttat cagctcatac tctttcattt ctaagaaaag
     4801 cgaacaactg aattctttag cttcatagat gataacgtaa caactggcaa ttctgctttt
     4861 aaaatggtct ttaggacctt catgaagtac aaaacttaca tcacaaacgc cctcagctat
     4921 ccctttccaa tgccaaatta gaagcgacca acaagcttat caaagacatc aagcgacaag
     4981 cctttggctt tcgcaacttt aaaaatttta aaagcaaaat tctcatcgct ttgaacatcc
     5041 aaaaagagag agaaccaagc tgattctctc tcgttgttaa tgataagtca cccattacag
     5101 ttgacaaaga gcccatataa ggtgtacagg tcagaagggt aacataatct ttatcctctt
     5161 caatgctcac ggcatccaat tggctaggtt caatcaccat gatacgatcc acttgataag
     5221 ccaaggtttc tttcagattg gtaacataaa aataatcacc ttttttcatc ttatccaaat
     5281 ccgcaaacaa ccgagcagct ggcatacctc tttgaccgct caagaccgca tgtgtagaag
     5341 cccctccaat cggcaaactt atcccttcta gatgaccaac tccccgttgc agattgtcct
     5401 cttctgaacc agcgtagatt ggtaaatcct gattgatttt tggaatcatc acatggtcaa
     5461 cctgctctct gacttctaac atcctagcgt attcagtcca ccctgccttt tttgctcctc
     5521 agaataagaa tctctcagca cagattgagt cgactgacta cttgttccag aaatactagc
     5581 attgtaggct ttagccagac ctaaccgtcg ttgaatctcc gtacggtcaa tggttgtaac
     5641 tgttctttta aaggcattaa tattggcatg agaagcttga taataactaa tctgactgac
     5701 aatagggaaa ggccaaaact agtcattttc tattatttaa taatttcaac gacaccttac
     5761 aattgtttac gacggaaaga acaaatggta agtatcataa aagtagaacc aactaatata
     5821 tagacaattg taccaattcc ccctgtttta ggaaaaacac ctggtgggcg tttgggagtg
     5881 ttggtaataa gatgtttacc atttccttca agatacccga tttgattttt attagcattt
     5941 ggatctgctt tcaggttcgt aacttctcca ttttgaattg taaatgtcac aacaggtttc
     6001 gttttaacct ctatatagcc atctggactt gaaatttcat ataatttata gttaccatct
     6061 tgaagtgctt taaaataaat tttaccatca ttctttgttg taacatcact tccctctgga
     6121 acaaattgct tatacccaga aaaatctttt tctatctgaa gttgaaactt agctcccaaa
     6181 agcgattctg aatgtttgtc tttattaact ttaataaatt caacctcacc cattttcttc
     6241 tgattactga tggttagtac cggaaactca cgaacatcac gaattttggg aattgggaaa
     6301 tcacgaatag tatttggttc tttttcactc ttcggactta gcgttgtacg attatttgta
     6361 ttgtaaaatt tgttacttat atagttatct tttaaacgta catcataggt aagaactact
     6421 ttttgtccac ttcctaagtt caaatgattg attttgatgg tttgagatgt cttatcataa
     6481 gtcactgtaa catcttttaa aattccccca tcactgtttg gtccaccaag agccacacca
     6541 ttttttaatt gactgccatc atttccaacc aaaacgtaat catcatgtgt aaaactttga
     6601 ccatttttta attggaattc aatcatctct cccataggat cagtcacatt tccatcaa
//
LOCUS       DATRNX010000099         6642 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRNX010000099 DATRNX010000000
VERSION     DATRNX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            83..568
                     /locus_tag="VBC81_001634"
     CDS             83..568
                     /locus_tag="VBC81_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912660.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            667..1320
                     /locus_tag="VBC81_001635"
     CDS             667..1320
                     /locus_tag="VBC81_001635"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6912661.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1386..2024)
                     /gene="plsY"
                     /locus_tag="VBC81_001636"
     CDS             complement(1386..2024)
                     /gene="plsY"
                     /locus_tag="VBC81_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6912662.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            2138..4087
                     /gene="parE"
                     /locus_tag="VBC81_001637"
     CDS             2138..4087
                     /gene="parE"
                     /locus_tag="VBC81_001637"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6912663.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            4221..>6642
                     /gene="parC"
                     /locus_tag="VBC81_001638"
     CDS             4221..>6642
                     /gene="parC"
                     /locus_tag="VBC81_001638"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6912664.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDK"
BASE COUNT         2270 a         1040 c         1319 g         2013 t
ORIGIN      
        1 ttgcgatttc aatagtgata tggtatattt aaagtaagct acgtatagtt ttaccagtca
       61 gaaatagtaa ggatggttga gtatgagaga aactgaaaca cacgatcacc aagcgctaat
      121 tcaaaaattg ttagtcagta ttcattattt aaccttgttt cgtgatgaaa ttatattagt
      181 agaaaaaacc ccatctttac tggggaaaca tttttctatt gctattgtgc agaatgaact
      241 aggtgagata ctttctaaaa tagaagcttt gagtaagcaa aaaaaactga ttcgttctat
      301 ttattggtat gatgaatcgt catttaaagt catgaataag gctctggcta ttgtagaaga
      361 atggattaag ggcttagata atcttttaga attctgtcaa tcacagacgg tttttcaagc
      421 aattttaggt gatgaacgtg cacatgtatt tggaatcctt attgatgttt atacttcttt
      481 aaacattata aatacttctt tgaaagaaga aaattctaga ccagtgtcct tatctgattt
      541 ggctctaggt cttaaggacg aagattaata aaaaaacgtt actgctttga atgaataaag
      601 cagtaacgtt ttttataaaa tattgctata ctagtaatac agatatctaa aagtaaggag
      661 ttataaatga aacattccag ttggcatgat ttaataaaaa gagaactacc caatcattac
      721 tacaataaaa ttaatacatt tatggatgca gtttacgaaa gcggaatcgt ttatcctcca
      781 agagataagg tgttcaatgc catacagata acaccactag aaaacgtaaa agtagtgatt
      841 attggccaag atccttacca tggccctcaa caagctcaag gactctcatt ttctgttcca
      901 gacaatttac cagctcctcc ttcacttcaa aatattctaa aagaattagc agaagatatt
      961 gggtcacgtt cacatcatga cttaacatca tgggcgcaac aaggagtgct gctattgaat
     1021 gcttgtttaa cagttccaga acaccaagct aatggtcatg ctgggcttat atgggaacct
     1081 tttactgatg ctgtcatcaa agtggttaac caaaaggaaa ccccagtagt ctttattctg
     1141 tggggtggct atgctcgaaa gaaaaagtcg ctaattgata atcctattca tcatattatt
     1201 gagagtcctc atccgagtcc cctatctgct tatcgtggtt tctttggtag cagacctttt
     1261 tctagaacaa atcatttctt agaagaagaa ggaataaatg agattgattg gttaaattag
     1321 aagggaaatc tactctcgaa acattgttag tcttataata aaaaaccgct agtagcggtt
     1381 ttttattatt tatttttttg tttacttagg tttaagccaa acggaaccaa attttcttgc
     1441 ctttttcgaa tacgtttgat attatcctga tgtcggataa taatcgtgag ggccattaag
     1501 ataaccacag tggtaaatat ccagtcatag tccgttaata taaaacctac taatggaaaa
     1561 attaagacac taaggatacc tacgacagct actgtaatac tggatagcga aatcatgcta
     1621 aaaagataaa gcgttaataa aaagatcact aataagtaaa ggaaaaaaga aggtgcaaat
     1681 cctagcaaaa ccccagcgct tgtagcaacg gctttcccac ctttgaattg tgcaaaaata
     1741 gggaaagtgt gacctataat agcaaaaaaa ccgataaaaa atggagatac tgtcgttaca
     1801 cctaatatga taggaataag tgttgctaga gtccctttta aaatgtcaat agtcaaagtg
     1861 acaatgccag cttttacacc taaaatacgg aaggtgttcg tggtgccagt attaccacta
     1921 ccgtgttgac gaagattgac ttggtaaaaa tatttaccaa tccaaagccc agtttggata
     1981 gagcctaaca gataggcaat gataatcata attataatat tcataccctt attataacat
     2041 aagcataaaa gtctttttgc tatttttaaa aaaataattt tgcaaaaaca gcaaaaaact
     2101 tgtaatataa aaaagatgaa aaaatttgga ggtcactttg actaaacaag atataacagt
     2161 gacgaattat ggcgacgatg ctattcaagt cttagaagga ttagatgctg ttcgtaagcg
     2221 tccagggatg tatatcggtt ccacagatgg aactggttta catcatttag tgtgggaaat
     2281 tgtagataat gcagttgatg aagctctatc aggttttgga aatcgcattg atgtgattat
     2341 taacaaagat gggagtataa ctgttacaga ccatggacgt ggtatgccaa ctggaatgca
     2401 tgctatggga aaacctactg ttgaagtcat cttcacggtg cttcatgcag gtggaaaatt
     2461 tggacaaggt ggttataaaa catcaggtgg tctccatggt gttggatcat ctgtagttaa
     2521 tgctttgtct tcttggttag aagttgaaat cattcgcgac ggagctattt acagacaacg
     2581 ttttgaaaat ggtggtaagc ccgttacaac tctgaaaaaa attggcacgg caccaaaatc
     2641 aaaatctgga acttctgtca gctttatgcc agatcaatca gtattttcaa cgatagattt
     2701 taagtttaat acaattgcag agcgcttaaa ggaatctgcc tttctcttaa aaaatgttac
     2761 tttaacctta acagataatc gttcagaaga agcagagcat ctagaatttc attatgaaaa
     2821 cggtgttcaa gattttgtag agtatttaaa tgaagataaa gaaacgttga caccaatcat
     2881 gtttttcgaa ggtgaagaac aagaatttca tatcgaagtt gctttgcagt ataatgatgg
     2941 tttttcagat aatatccttt cttttgtcaa taatgttcgt actaaagatg gtggaacaca
     3001 tgaaactggc ttgaaatcag ctattacaaa gtctatgaat gactatgcac gtaaaacagg
     3061 acttcttaaa gaaaaagata aaaaccttga aggttctgat tatcgtgagg ggctatcagc
     3121 cattttatct attcttgtac ctgaggagca cttgcaattt gaaggtcaaa ctaaagataa
     3181 actaggaagt cccttagcac gaccaattgt tgatggtatt gtttctgaaa agttaactta
     3241 ctttcttatg gaaaatggag atttagcatc taatttaatc cgtaaggcaa taaaagcacg
     3301 tgatgcgcgt gaagctgcac gtaaagcacg tgatgaaagt aggaatggga aaaagtcgaa
     3361 aaaagataaa ggtctacttt ctggaaaatt aacccctgca caatcaaaaa atgctaagaa
     3421 aaatgaactc tatttagtcg aaggagactc tgcaggtggc tctgctaagc aaggtcgtga
     3481 tagaaagttt caagctattt taccgttgcg aggaaaagta ttgaatacag ctaaggcaaa
     3541 gatggctgat attataaaaa acgaagaaat taatacaatg atccatacca ttggtgcagg
     3601 tgttggtcct gattttaatc ttgacgatat taattatgat aaagttatca ttatgaccga
     3661 tgctgatact gacggagctc atattcagac actattatta actttctttt atcgctatat
     3721 gagacctctt gttgaagagg gacatgtcta tattgctttg ccacctttgt ataaaatgtc
     3781 aaaaggaaaa ggtaagaaag aaattgttga gtatgcttgg acggatatag agttagaaga
     3841 attacgacaa aaatttggta aaggctcact attacaacga tataaagggc ttggagaaat
     3901 gaatgccgat caattgtggg aaaccactat gaaccctgag acaaggacat taattcgtgt
     3961 aacaattgaa gatttagcaa gagcagaacg tcgagttaat gtcttgatgg gtgataaagt
     4021 tcctccaaga cgccaatgga ttgaagataa tgtcaagttt actttagaag agaatacagt
     4081 attttaataa agagccttgg ttattcatgt tttatagtgt acaagtaata ttgtatgcgt
     4141 tagacaagag agccaatcat tcatttcaaa taagtggaaa aataatacta agccactcca
     4201 actaggagaa aattaaaaat atgtctaata ttcaaaatat gtcccttgag gacattatgg
     4261 gagagcgttt tgggcgctat tctaagtata tcattcaaga acgggcgttg ccggatattc
     4321 gtgatggctt aaaacctgtt caacgtcgaa tcctttattc aatgaataaa gatggtaata
     4381 cttttgaaaa gggatttcgc aaatctgcaa aatcagtcgg taatgttatg gggaatttcc
     4441 atcctcatgg ggattcctct atctatgacg cgatggttcg tatgtctcaa gattggaaaa
     4501 atcgtgagac attgattgaa atgcacggaa ataatggttc tatggatgga gatcctgcgg
     4561 cagctatgcg ttatacggaa gcccgtctat cagaaattgc tgattatctt cttcaagata
     4621 ttgataagaa tactgtaccc tttgcgtgga attttgatga tacagagaaa gaaccaactg
     4681 ttttaccagc tgcctttcct aatcttttag tcaacggagc aacaggaata tctgcaggct
     4741 atgcgacaga tattccacca cataatttag cagaagtcat tgatgctgtc gtgtacatga
     4801 ttgatcaccc taaagctaaa ttagataaat taatggaatt tctacctggt ccagattttc
     4861 caactggcgc tatcattcaa ggaaaagatg aaattcgtaa ggcatatgag actggtaagg
     4921 ggagagtagc ggttcgctcg cgaactgcta ttgaaacctt aaaaggtggt aagaaacaaa
     4981 ttattgttac tgaaattcct tatgaagtta ataagtcagt tttagtaaaa cgaatcgatg
     5041 atgttcgtgt taataataaa gttccaggaa tagcagaagt gcgtgatgag tctgatagag
     5101 atggccttcg tatcgctatt gagctcaaaa aagaagccga tgaaacaatt gtcttaaatt
     5161 atctttttaa atatacagac ctacaagtta attataattt caatatggtt gctattgatg
     5221 actatacccc taagcaggtg ggtctatcaa gaatattaac tagctatatc gctcaccgaa
     5281 gagaaattat tattgctcgt tcaaaatttg ataaagaaaa agccgaaaaa cgtctccata
     5341 ttgttgaggg actgattagg gttctttcta ttttggatga agtgattgca ttaattcgtg
     5401 cttctgagaa taaagctgat gcgaaagaga atttgaaggt cagctatgag ttttcagaag
     5461 ctcaagcaga ggctatcgtc acattgcaat tatatcgtct cacaaatact gatattgtga
     5521 cgttgagaga agaagaagaa gaacttcgtc aacaaatcac aatgctcaag gcaattatta
     5581 gtgatgaacg tacgatgtat aacgtaatga aacgtgagtt acgtgaggtc aaaaagaaat
     5641 ttgccaatac tagacgttct gaacttcaag aattagctga aactattgaa atcgatactg
     5701 caagtttaat tatcgaagag gacacatacg tcagtgttac tagaggcgga tatgtaaaac
     5761 gcaccagccc tcgctctttc aatgcatcaa ccgttgatga acttggtaag cgagaagatg
     5821 acgaattgat atttgtgagc aatgcaaaga ctacccaaca tttattgatg tttactaacc
     5881 ttggtaatct tgcttatcgt ccagttcatg aattagctga tattcgatgg aaagacgttg
     5941 gtgaacattt atcacaaaat cttgttaact ttgcttcaaa tgaagaaatt atttatgctg
     6001 aattagtaga tgattttact aaagaaactt attttgctgt cactagttta gggcaaataa
     6061 agcgttttga acgtcaagaa atcagtccat ggcggacata taagtcaaaa acagctaaat
     6121 atgctaagtt aaaaaatgta gatgattacg ttgtgactgt tgctcctatc caattagagg
     6181 atgttatact tgtcacttat aatggttatg tgttgagatt tagtataaat gatgtaccag
     6241 tcgttggtag taaagccgca ggtgtaaaag cgatgaattt aaaagatagt gatcacatcg
     6301 tatctgcatt tatagctaac accacaagtt tatatttatt aactcataga ggaagtctta
     6361 aacgaatggc cattgatgtt atccccacaa ctagtcgtgc taatcgagga cttcaagttc
     6421 ttcgtgaatt gaaatccaaa ccgcatcgcg tttttaaagc aggacctgta tatttagagg
     6481 attcatcttt tgaatttgat ttatttagtt cagtatcgaa tcatgaagga gatactttcg
     6541 tacttgagat tatgtctaaa acaggaaaag tttacgatgt tgatttaagt caatggagtt
     6601 tttctgaacg tacaagtaat ggctcttttg tatctgataa ga
//
LOCUS       DATRNX010000100         6587 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRNX010000100 DATRNX010000000
VERSION     DATRNX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            443..694
                     /locus_tag="VBC81_001639"
     CDS             443..694
                     /locus_tag="VBC81_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912665.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
     gene            713..967
                     /locus_tag="VBC81_001640"
     CDS             713..967
                     /locus_tag="VBC81_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6912666.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(1304..1498)
                     /locus_tag="VBC81_001641"
     CDS             complement(1304..1498)
                     /locus_tag="VBC81_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912667.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            1497..1691
                     /locus_tag="VBC81_001642"
     CDS             1497..1691
                     /locus_tag="VBC81_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912668.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(1742..1918)
                     /locus_tag="VBC81_001643"
     CDS             complement(1742..1918)
                     /locus_tag="VBC81_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912669.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(1954..3543)
                     /locus_tag="VBC81_001644"
     CDS             complement(1954..3543)
                     /locus_tag="VBC81_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6912670.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            complement(3604..3672)
                     /gene="shp2"
                     /locus_tag="VBC81_001645"
     CDS             complement(3604..3672)
                     /gene="shp2"
                     /locus_tag="VBC81_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6912671.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            3760..4608
                     /gene="rgg2"
                     /locus_tag="VBC81_001646"
     CDS             3760..4608
                     /gene="rgg2"
                     /locus_tag="VBC81_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO6912672.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            4757..5578
                     /gene="mutM"
                     /locus_tag="VBC81_001647"
     CDS             4757..5578
                     /gene="mutM"
                     /locus_tag="VBC81_001647"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6912673.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            5569..6162
                     /locus_tag="VBC81_001648"
     CDS             5569..6162
                     /locus_tag="VBC81_001648"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6912674.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            6286..>6587
                     /locus_tag="VBC81_001649"
     CDS             6286..>6587
                     /locus_tag="VBC81_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912675.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDL"
BASE COUNT         2111 a          996 c         1207 g         2273 t
ORIGIN      
        1 atttgttttc aacagagcgg atttgaccat ctgttgtttg ccatgccttt cataattttt
       61 cagtaattat gatagttgtt ctgttctccc aatcataaaa ctttaaataa gattctatta
      121 gttcttgcct cactctcctt aagtttctgg taaaaatcga aaagttacca gactttaaag
      181 gggagttttt tatttgtcaa cagttagtag aaaaacgata tattgctcca tttagattaa
      241 ttatggagat aaccaaataa tagcttgtga taagaagata cctttgaagt ttcagaaaca
      301 atctgttttc catcctgcta tattcatctt tgccaaagtt gcaacaacac aataataaca
      361 attgcttggg cattgggtga ttagtttact atcaataggg ttagagtgga gttggagtat
      421 atggattggc attaatctta taatgatgat tctgttgagt tatgtgctaa caaaaactga
      481 gccaatcagt aactaccaag agtcatcatc taacctcaaa aaatggctat ttgcaatagg
      541 tgcttttagc gttttatttt gctgggcact actagtaaaa acatttttcc caataaggca
      601 aaaccaggag ggattaatag ctgatatgaa tcaggtcaat cactactttc gagttgtctt
      661 attattgact atctgtctta ttgccccaat ttaagaagag ataacgttca ggttgtttgt
      721 catgagaaca tttgggaact ccctgagtgg tcccttggtt gtgatcttat cttctatact
      781 atttagctgg tctcacttac atggtctatc tgtcgtcgac tttgttgtct attttggtat
      841 gggtttgatt ttcgctagtt tgcaccacta tacaaaatcc attcattatt cgattggcga
      901 gcatattgtt tggaatagtt tgtcatatat tttttatttt ctagcatttt tgcttgattt
      961 actctaagta ataagattga gagactaatc attactaaga catttttgtc agtttaaaag
     1021 agctttttgg ctcttttgtt attcgttttt ttaatgaata aaatgttatt attttattgt
     1081 taactaaata aagttcgtaa ctttatatga tttatataga attacgagtt ttttgttaga
     1141 taaatatgag aaaatatagt atgacgaatt cctaaataaa aaataatgaa aggaacatga
     1201 tgttaaaaga tgtgaatgtt ggaatgagtc atttattagt tagaggtgct attattctag
     1261 tagtagggat tgtttttctg ttgcaacaga ttcagtaagt acttcaagtg ggagctttgg
     1321 aataggttat cgagaagaaa agacccagac gttctctttg gatcagctgt cccaagtgat
     1381 tttgtattgc ggggagccca atactgttag atttacttat acaaatagct tctatatgat
     1441 gaaagatatt tatatcgctc tggatggtgc tggtagctct ttgaccactc atgatcatgc
     1501 tgttcctaat gattaaacca gattatataa ggtatttcaa agtcattgac gatatgcatg
     1561 ataccatgta ccaacagact gtttttcatg ttcaactgca agcttggaaa tacaccatga
     1621 aatcgattga tcaattagtt gtagatgatt tgaatcatat cagtaatgag aaaaccactt
     1681 tggttaaata aaaaggaaca ctgtcaaaaa tggcagtgtt cctttttatt ttaggagaga
     1741 gctaagattg gtcatctaat gggatttcca catgttcaac tgtcatagta atgacaggtt
     1801 tgataacaca atctctgtct ttagggatag tctcctgttg cagaggcgcc tcatcgctcc
     1861 aaattttctt gtcagaacaa ttggaatgta gataatttga atcagtcttt ttagacattt
     1921 catatccttt ccaggctcaa tacaattttt gagttatgca ccttgatggc gatcagctgg
     1981 aatatcttta gctaatgcat tgtattgact atccaaaaga tgatcagcat ccattattgt
     2041 tttaccaaca aggaaatatt tattgctttt tatattatta tcccatgttg tatcaacata
     2101 atatttaacg ccattaatag taacaatgtt ccaagcgtga tttccacctc cagcttgacc
     2161 agttacatac catactgata agccagcagc agccatatct ttaaacatta ctgcaaaggc
     2221 ttgacaaaca ccacgtttat ctttgtagag tgcttcagga gcatggatac tgataccact
     2281 agcagtttca cgagtggttg ccagttcttc agtcgcgtaa gtgtaattat taaccaagtg
     2341 gtcatgaatt actttagctt tttcataatc acttgtgata tttttgttag taatattttc
     2401 tttgacaaaa gaatctacaa actgtttata ttctttatgt aatcctacca tgtcaccttt
     2461 aagagtatag gtgattgtga tgtcgctttt tacaataatg cgttggattt gtccaccaag
     2521 agtataagtg ctaatgttac ttttggcatt gaattcagtt gctgttccaa gtgtatctcc
     2581 aatttcccgg atgatgcttc cgagattttt ttggtattcg tcaacttgtt cttgagtttt
     2641 aaactccatt tgaacactga tgtgtggtac ttggttgcta atattatttc gtaaaacttc
     2701 ttttaaacta tcgtaatcgg caatattagc tgactcaggc tgtgatgcct ttgcatcaga
     2761 ggtagcctgt ttttcctcac tcttagttac ttcttgttta ttgcctgacg cttctgattg
     2821 agtagttttt tcttctactt tgtttgcagg aacagaagta ttttcgctaa ctttgagagt
     2881 agcaagttta gaagtctcag tttcaatagc cgctgttgat agggcatcta cttcttgttg
     2941 aaggattgcc atagcagctt tgtaattatt aacttcagct acaagaggtt caatttgatt
     3001 tctgagtgaa tgaaataaat tttcagcttc ttgtatagaa ttcacagagt tgtagtttac
     3061 agttgctaaa atagcttgaa ttttgttaac aacctgatta cgttgcccca cgagctgagc
     3121 tgctttattt tgacttgtct gaatcttatt atgattttgg tcaatcagat tttgaacagt
     3181 ttggttaatg acattacgtt gattaacgat agcattatag ttactgggag agagatgacc
     3241 agctttcacc tggttaaata attgctcatc tttttgttta aataatgtta tagctctatt
     3301 taactgttca atagttggat tagcatttag ttgagtgaat gagttgacat tatcaacaac
     3361 aaattgattg agcaaacgat gttgttcctt gatgtaacta ataagctgct tttcattatt
     3421 ttgcttactg atatagtgat actcactggt ggtgtcatca gctttaacct catagttaat
     3481 agttgatagt gaactgataa ttaaagctgc ggcaaagcca ctaataattt ttttaatctt
     3541 cattagttct cctgttataa actaaataaa atgggattcc ataacaacca tttgtgggga
     3601 gttttatcca ccaacgataa ttaggatatc catgattaat gtgaacaaca aagctttgtt
     3661 aatttttttc atgactatct cctttctgat tttctatttt gtacttgtat tatataatgg
     3721 atttaaaggg tcttttaaat tgttgtgaaa gtgggaaaaa tggaaaaaga attaggaaaa
     3781 acactaagaa gattacgtaa aggaaaaaaa gttagtattt cttctttagc agatgagcac
     3841 ctatccaagt cacaaatctc aaggtttgag agaggagagt cagaaattac ttgtagtcgt
     3901 ttgcttaata ttttagacaa actcaacatc actattgatg aatttgtgtc gattcattct
     3961 aaggcgcaca ctcatttttt tatcctttta aatcgagtaa gaaaatattg tgctgaaaaa
     4021 aatgtaacta aattagtggc tctcttagag gatcataacc ataaagacta tgaaaagatt
     4081 atgattaagg ctcttatttt ttcaattgat caatcaatag agcctaatca agaagaatta
     4141 gcacgcttaa cagactatct ttttacggtg gagcagtggg gatattatga aattattctc
     4201 ttaggaaatt gctcacgtct cattaattac aatactttat ttttattgac caaagaaatg
     4261 gttaattcgt ttgcttattc agaacaaaat aaaaccaata aaatcctagt tacacactta
     4321 gcaattaatt gtctaatcat cagcattgat cactcttact ttgagcatag ccactactta
     4381 attgataagg ttagatcatt gttacaggat gaagttaatt tctatgaaaa aactgttttc
     4441 ttatatgtaa ctggatacta tcatttaaaa cttggtgata cttcttcagg aaaagaagat
     4501 atgaggaaag ctttacagat ttttaaatat ttaggtgaag attcatttta caatacttat
     4561 aatgaacatt attgtcaaaa agtacttggc aataataaag aatgctaatg agtgcaagtt
     4621 tatgagtaat ttttaaagct tttgtctcaa aatgataata agaaaggaaa atagaaatcg
     4681 aatggtcagg tgacatcaag aacgtttgga aattattgct ctaagcaatg actattgatt
     4741 tttagactat atatttatgc cagaattacc agaagtagaa accgttagaa aaggactaga
     4801 aagacttgta gttaatcaag aaatagcttc tataacgatt aaagtcccta aaatggtgaa
     4861 aactgatttg aatgacttta tgataagtct accaggaaaa acaattcaac aggtattgag
     4921 acgagggaag tatcttcttt ttgattttgg tgaaatggtt atggtatcac atttacgcat
     4981 ggaaggaaaa tatctactct tcccaaataa agtaccagat aacaaacatt ttcaccttta
     5041 tttcaaattg actaatggca gcacattggt ttaccaagat gtccgtaaat ttgggacatt
     5101 tgagttggtt cgtaaatcat ctctcaaaga ttattttact caaaaaaaat taggtcctga
     5161 gccaactgct gacacatttc aatttgagcc tttttcaaaa ggcttagcaa actccaaaaa
     5221 gccaataaaa cctttactat tagatcagag attagtagca ggacttggaa atatttacgt
     5281 agatgaagtc ttgtgggcag caaaaattca cccacagcgt ttggctaatc aattgaccga
     5341 atctgaaaca agcttattac acaaggaaat tattcgcatt ttaactttag gcatcgaaaa
     5401 aggtggctcc acgattagaa catataagaa tgctcttgga gaggacggta cgatgcaaaa
     5461 gtatttacag gtctatggta aaactgggca accgtgtcca aggtgtggtt gtttaattaa
     5521 gaagataaaa gtaggaggga gagggacgca ttattgccct cggtgtcaat gcttatgaca
     5581 aaaataatag gactgacagg agggatagct tctggaaagt caacggtaac aaaaataata
     5641 cgagaatcag gttttaaagt catagatgcg gatcaagtgg ttcataaatt gcaagctaag
     5701 ggtgggaaac tttaccaagc tttattagaa tggttgggtc ccgagatact tgatgctgat
     5761 ggtgagttgg atagaccaaa gctttctcaa atgatttttg ctaatccaga caatatgaag
     5821 acatcagcta ggctacaaaa tagtatcatt cgtcaagagt tagcatgtca gcgcgaccaa
     5881 ttaaaacaaa cagaagagat atttttcatg gatattcctt tattgattga agaaaagtat
     5941 ataaaatggt ttgatgagat ttggttggta tttgttgata aagaaaaaca attacaacga
     6001 ttaatggccc gtaacaacta cagtcgagaa gaagcagaat tacgactttc acaccaaatg
     6061 cctttaacag ataaaaaaag tttcgctagt cttattattg acaataatgg tgatttaata
     6121 actttaaaag agcaaatatt ggatgctctt caacgtttat agaactacta aatggataat
     6181 gtatttatcc attttttgtc tttattacac tagttttaaa aaacttgcga ttaaaccaca
     6241 gtaatagtac aatgatataa aaggtttact ttcaaaggag tttttatgga gttagtaatt
     6301 agagatattc gtaagcggtt tcaggaaaca gaggtcttga gaggagcaag ttaccgattt
     6361 tattcaggta aaataacagg ggtcttaggt aggaatggtg ctgggaaaac aactttattt
     6421 aatatacttt atggggatct tgcagctgac aacgggacca tttgtttatt gaaggataat
     6481 cacgagtatc ctcttaccga taaggatatt ggtattgttt attccgaaaa ctaccttcca
     6541 gaatttttaa cagggtatga atttgtaaaa ttttacatgg atttaca
//
LOCUS       DATRNX010000101         6557 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRNX010000101 DATRNX010000000
VERSION     DATRNX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            166..1122
                     /locus_tag="VBC81_001650"
     CDS             166..1122
                     /locus_tag="VBC81_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO6912676.1"
                     /translation="MTNFVCHTLYHLLITIIKLKDKENTRIFICDTITDYETWVKILN
                     DQGIRTESFKEFAYREQLQNKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYM
                     VYLGKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLV
                     GLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDK
                     CYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNVP
                     MEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            1119..2519
                     /locus_tag="VBC81_001651"
     CDS             1119..2519
                     /locus_tag="VBC81_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6912677.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDTIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2519..3544
                     /gene="neuB"
                     /locus_tag="VBC81_001652"
     CDS             2519..3544
                     /gene="neuB"
                     /locus_tag="VBC81_001652"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6912678.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3621..4775
                     /gene="neuC"
                     /locus_tag="VBC81_001653"
     CDS             3621..4775
                     /gene="neuC"
                     /locus_tag="VBC81_001653"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6912679.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4772..5401
                     /locus_tag="VBC81_001654"
     CDS             4772..5401
                     /locus_tag="VBC81_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6912680.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5412..>6557
                     /gene="neuA"
                     /locus_tag="VBC81_001655"
     CDS             5412..>6557
                     /gene="neuA"
                     /locus_tag="VBC81_001655"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6912681.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNY"
BASE COUNT         2152 a          962 c         1226 g         2217 t
ORIGIN      
        1 tgcttataga atgtatggac ggttacttag aaaagtgaag ttagttgata aattaaaact
       61 tataaaaaat aggtttttct agaaatggct gtttaatagt tgtaatgtga gtgaccacga
      121 attgttggat aactatcaaa aagactaatt taggggaaaa ataaaatgac taattttgtt
      181 tgtcatactt tataccatct actgattact ataataaagt taaaagataa agaaaatact
      241 agaatattta tttgtgacac tatcacggat tatgaaactt gggtaaagat attaaatgat
      301 cagggaataa gaactgaatc atttaaagag tttgcttatc gggagcagtt acaaaataaa
      361 aatattgaag aagtgatgga gcttgtagat tcagacttaa atcactactt tgagagagta
      421 gatacacaag tctatctatt taatgatgat actttaatag gtcgctatat ggtgtattta
      481 ggtaaaaatt atcatctaat tgaagatggt tataactgtt ttcaagccaa gttattcttg
      541 ggtggttctg tcgtaaaacg agtaattaaa acttacttat ttaaaaagta tgttccgtat
      601 ggtttttcaa aatattgtct ctcaattgag gttaattctt tagtggggtt acctcatgac
      661 ataaggagta agaaatataa agaactaccg agaaaaaaat tatttgatag tcttaacaaa
      721 gaacaaaaat cactgatttt caaaatattt aaaacaaaac cattaactat aactccaaag
      781 tcagtattat tgttgacaca gccacttgca caagataaat gttataaaac acctacagag
      841 aggtttcaaa gtattcaaga gcaatacgat tattttgacg atattgtcca ggaatataga
      901 acgttagggt acaatgttta tttaaaagtt catcctagag atgtagtaga ttattccaaa
      961 ttgccggtag agctattacc atcaaatgtt cctatggaaa ttatagagtt gatgtcaaca
     1021 ggtcggttcg aatgtgggat aacacattcg tccactgcgc tggatttttt aacttgtgtt
     1081 gataaaaaaa taactttagt agatctgaag gatattaaat gaatactaag aaattacttc
     1141 aatctggttt tatttataca ctcgggaatt tattggtgca aggattagcc tttatcacac
     1201 ttcccatcta tactcgggtt atctcaacag aagtttatgg ccaatatagt ttatatgtcg
     1261 cttggatgaa tattattatg ctttttattg gccttcagac gagcggttct ttaagttcag
     1321 cccgggtcaa atacggagaa gaatttaaaa gctattcagg gagtgccttt tctgtaggta
     1381 atatatggtt tcttattata cttttgatag cttttctatt tagaagtttt cttgcaccat
     1441 tagttggttt ttctgaatct atttttttat taatggtgtg tcaaagttac gctagctatg
     1501 tggtgacttt ctttggtcag tattttatac aacaacagag gagtttggct aatttaatat
     1561 tatccttagc caatgcagtt tcatctgttg cactatctct atttttaatt tttcattggt
     1621 ccgatgactt tttatctagg gtttttggag cttttgttcc tactataata actggaatag
     1681 ttgcctttgc ttatatttat tatcatagca aatcttttta caatcctaag tattttcggt
     1741 tcattgtcac tgtgtctgtt cccttgattt tccatttgtt aggacaccag ttgttggggc
     1801 aattagatag aattatgcta gctcgtttat acaatactaa agaagttgca atgtatagtt
     1861 tcggttattc gcttggaatg attattcaga ttgttttgaa tagtattaat atggcttgga
     1921 ttccatggtt ttttgatgct agaaaagaaa aattactgca actttcaact tatattagcc
     1981 gttatctcta cttaggagtt ttcttaacat taggttattt gactgttttt cctgagttag
     2041 ctcaaataat gggaggagac aagtatagta gtagtgttca atttatttct ttaattattg
     2101 tcagctactt tttagttttt ctctatacat ttcctgtaaa tatccaattc ttctatgcta
     2161 atacgacatg gattcctatt gggacattat tagctgctgg tgtaaattgg ttactaaacc
     2221 ttgtattaat tcctcattat gcagcctatg gcgctgctat ggctacaata atatcttatt
     2281 tagccttgtt gatttttcat catattgttt cgaaagttaa ataccattat tcagatgtct
     2341 cagttaggca gtacatcatt ttaagtggta tagtatttag ttatgctatg ttaatgaaca
     2401 tgttcctaga tactatcgtt attagatgga gtttaggaat aattattctt atagtatata
     2461 gcattgtttt tcaaaaagtc attttagact tattgagtaa aaaaagaagg agaagataat
     2521 ggtttatatt attgcagaga ttggttgcaa tcataatgga gatattaatc ttgcgaaaaa
     2581 aatggtagat gttgccgtgt cttgtggtgt tgatgctgtt aaatttcaga cttttaaagc
     2641 tgagaaactt atttctaaat ttgctcccaa agctgaatat caaaaagcaa ctacaggaac
     2701 agcagacagt caacttgaga tgacgaaacg tttagagtta agctttgaag aatacttaga
     2761 aatgcgtgat tatgcaattt caaaaggtgt ggagaccttt tcaacacctt ttgatgaaga
     2821 gtcattagag ttcttaattt ctacagatat gccaatttac aaaattccat caggagaaat
     2881 cactaattta ccttacttag aaaagattgg caagcaacaa aagaaagtta ttctttcgac
     2941 gggtatggcg gtaatggaag agatccatca agcggtgaat attttacgtc agaatggtac
     3001 aaccgacatt tctattttac attgtacaac agagtaccca acaccttacc cctctctaaa
     3061 tttaaacgtt attcatactt tgaaagatga atttaaagat ttaacgatag gttattcgga
     3121 tcattcaatt ggatcagaag tacctatcgc agcagcagca atgggtgcag aagttattga
     3181 aaaacacttt actttagata ctaatatgga aggtccggat cataaagcca gtgcaacacc
     3241 tgatatttta gctgctttag ttaaaggggt tcgcattgtt gaacaagcct taggtagatt
     3301 tgaaaaaatc ccagatccag tagaagagaa aaataagatt gttgctcgta aatcagtcgt
     3361 tgctttaaaa ccaattaaaa aaggcgatat ttattcaata gaaaatatta cggtgaagcg
     3421 cccaggtaat ggtatttctc ctatgaactg gtatgacatc ttgggacaag aagcgcaaga
     3481 tgatttcgaa gaggatgaag ttattcgtga ttcacgcttt gaaaatcaat tgcccgagtt
     3541 ataataacac ctaattataa tcggcaacag atgtattaca cggctaagat tatgtaatta
     3601 ctatatatag aagggatacg atgaaaaaaa tttgtttagt aaccggatcg cgtgctgaat
     3661 acggaataat gaagccattg attcaaagat tatcaaaaga taaagaagtc aacttacaaa
     3721 ttattgcaac agcaatgcat ctggaagaaa agtacggcta tacttatcgt caaattgaag
     3781 aagacggttt tgatattgct tataaagttc ccttacatct ttatgatact gacagaagaa
     3841 ctgtatctac tgcaatggcg catttacaac taggattgac caaaattttt gacaaggaag
     3901 actatgatct agtcatcatt ttaggggatc gttacgaaat gttaccagtt gtgaatgtag
     3961 cgttgattta taatgtccca gtatgccacc ttcatggagg ggagacctca ttaggcaatt
     4021 ttgatgagta tattcgccat gcaattacta agatgagtca cctacactta gtctctacag
     4081 aggactttcg tcaacgtgtg attcagatgg gagaacaacc tcaatttgta attaacacag
     4141 gagctctcgg agtggaaaat gctctatcaa tcccttctct aaccaaagaa gcaatagaaa
     4201 agcaattagg tattgtacta gaggaatcct attttgttgt tctctatcat cctgttactt
     4261 ttgaacaagg aaaaagtgct ggagaacaga tgaaagcggt actatcagcc ctttctaaat
     4321 ttggtgtcca gtgtctattt ataggatcca attcagatac tggttctgat gatattgcaa
     4381 aggctatcaa tacttatttg ataaatcatg aaaatagtta ttgctttgct tctttatcaa
     4441 cccaacttta tcattcctta attcgtcatt cactaggact gattggtaat tcgtcttctg
     4501 gtttaataga agttccttct ttgatgaaac ctactcttaa tattggtgat cgccaaaaag
     4561 gtaggctaca tggagaaagt gtagtaagtg taccagtcga aaccccctct gtattggagg
     4621 gattatcaaa attgaatgaa gtgaccaact ttgacaatcc ctactacaaa gagaatgctt
     4681 catctattgc atatgaagca ataaagcttt atttgaaaga tgaaccttca atttcccaac
     4741 cattttatga tttaaaggag aataatctaa aatgaaaaaa aagttggcat ttttaggcgc
     4801 aggaagtcat gctgatgcga ttgctccggt tattgatcct cttgtgtatg atttcgtagg
     4861 tttttttgat gataaagata ttacggagca tgatggttat cctgttcttg gaaaacttta
     4921 tgatgtgcta ccttaccttg aagatggctc aatagatgca gtatttatta caataggtga
     4981 taatgctaaa aggaaagaac tatttgaata tgtagcaaag gattattatg actttattat
     5041 taacatcatt agtcccaatg ctttagtatt gacaccagat agtatttgtg ggcgtggtat
     5101 ctttattggt tttggggctt ttataggttc taaagtgaag ctgtttgata acaacgttgt
     5161 taatacagga gcgctcattg aacatcatac tgttgtagaa tcacactgta atatagcacc
     5221 taatgctacc ataaatggtc tttgttatat tagagaagaa gtttatgtag gtagtgccag
     5281 tgttattatt caaaccttgg atatttcatc gtgtacgaca attggcgcag gagctgttgt
     5341 tgtaaaagat attatagaac ctggaaccta tgtaggggtt ccagccaaaa aaataaaata
     5401 aaggatatgt tatgaagcca atttgtatta ttcctgcgcg atcagggtca aaaggattac
     5461 cagataagaa tatgttattc ctagcaggta aacccatgat tttccatact atagatgctg
     5521 caattgaatc tggaatgttt gataagaaag atatttttgt tagtacagat tcagaattgt
     5581 acagagagat ttgtttagaa cgcggtattt cagtggtgat gagaaaaccg gaactttcaa
     5641 ctgatcaggc aacttcgtat gatatgttaa aagatttttt atctgactat gaagataatc
     5701 aggagtttgt gttacttcaa gtaacctctc cactaagaaa atcatggcat ataaaggaag
     5761 caatggagta ttattcttca catgatgttg acaatgttgt aagtttttct gaagttgaga
     5821 aacaccctgg tctgtttacg acattgtctg ataaaggcta tgctatagat atggtgggag
     5881 cagataaagg ttatcgtcgc caagatttac aacctttata ctatccgaac ggcgctattt
     5941 ttatttctaa taaagaaact tacttaaggg aaaaaagctt tttcacctct aggacatatg
     6001 cttatcaaat ggcaaaggaa ttttcattag atgttgatac gagagatgat tttatccacg
     6061 tcatcggtca cctctttttc gattatgcca ttagggaaaa agagaataaa gttttttata
     6121 aagaaggcta tagtcgtttg ttcaatagag aagcttcaaa gataatttta ggtgattcaa
     6181 aaacgatatc tatctcacta gaaaattacc ataattattc ccaaggtggt gtaacattag
     6241 caacgatgtt ggaaaactta cctaactttt tgacagctaa tgtaacagag gcttttgttt
     6301 ctataggggt aaatgatctc attacaggtc atagtgtgga agaaatattc agcaatttcc
     6361 aaaaactcta ctcattatta gctgagaata aaataaagat gagattgaca actattgctt
     6421 acaccctttt tagagaaact gtcaataatg cagatattga gaaaatcaat caatggctaa
     6481 cagaattttg ttatcaaaat cagattccac tgttagatat taatagattt ttatcaaagg
     6541 atggtaatct aaattat
//
LOCUS       DATRNX010000102         6525 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRNX010000102 DATRNX010000000
VERSION     DATRNX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            22..93
                     /locus_tag="VBC81_001656"
     tRNA            22..93
                     /locus_tag="VBC81_001656"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Gln,seq:ttg)
     gene            102..173
                     /locus_tag="VBC81_001657"
     tRNA            102..173
                     /locus_tag="VBC81_001657"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:135..137,aa:Glu,seq:ttc)
     gene            236..754
                     /gene="rimM"
                     /locus_tag="VBC81_001658"
     CDS             236..754
                     /gene="rimM"
                     /locus_tag="VBC81_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6912682.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            741..1496
                     /gene="trmD"
                     /locus_tag="VBC81_001659"
     CDS             741..1496
                     /gene="trmD"
                     /locus_tag="VBC81_001659"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6912683.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1477..2469
                     /locus_tag="VBC81_001660"
     CDS             1477..2469
                     /locus_tag="VBC81_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6912684.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2606..3676
                     /locus_tag="VBC81_001661"
     CDS             2606..3676
                     /locus_tag="VBC81_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6912685.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3689..4612
                     /locus_tag="VBC81_001662"
     CDS             3689..4612
                     /locus_tag="VBC81_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6912686.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            4824..6458
                     /locus_tag="VBC81_001663"
     CDS             4824..6458
                     /locus_tag="VBC81_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6912687.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
BASE COUNT         2169 a          981 c         1349 g         2026 t
ORIGIN      
        1 gatatactag attttgtctt ttggggtata gccaagcggt aaggcaaggg actttgactc
       61 cctcatgcgt tggttcgaat ccagctaccc cagtttcaca tggtccgttg gtcaaggggt
      121 taagacaccg ccttttcacg gcggtaacac gggttcgaat cccgtacgga ctatcgtggc
      181 cagttgcatt ttagcggctg gtttttcttt tattttcaaa aatatcggag ttactatgga
      241 atactttaac gttggaaaaa ttgtcaatac acaaggatta caaggcgaaa tgcgtgtatt
      301 aagcgtgact gactttgtag aagaacgctt taaaaaaggt caagtgttag ctttattcga
      361 tgaaaagaat cagtttgtta tggatattga aattgctagt catcgtaagc agaagaattt
      421 tgacattata aaattcaaag gaatgtatca tattaatgat attgaaaaat ataaaggttt
      481 cacactgaag gtggcagagg atcaactgag cgaccttaaa gatggtgaat tttattatca
      541 tgaaattatt ggcttagatg tctatgaagg ggaagagctg attggaaaaa tcaaagaaat
      601 tttacaacca ggagcaaatg acgtttgggt tgtggaacgt catggaaaac gtgatctttt
      661 attaccctat atcccgccag tagtattaga agtggacctt tctaatcagc gtgttcaagt
      721 tgaattgatg gaaggtttag atgatgaaga ttgatatttt aactttattt cctgaaatgt
      781 ttgcccctct ggaacattct atagttggta aggcaaaaga aagaggactt cttgaaatta
      841 attatcacaa tttcagagaa aatgctgaga agtctcgaca tgttgatgat gagccttacg
      901 gtggggggca agggatgctt ttacgtgctc aacctatttt tgatacaatt gataaaatag
      961 atgctcaaaa agcacgtgtt attttgctag acccagcggg cagaacattt gatcaggatt
     1021 ttgcagaaga actatccaaa gaagatgaat taatttttat ttgtggtcac tatgaaggtt
     1081 atgatgaacg tataaaatca ttagtaacag acgaagtatc tttgggagat tttgttctta
     1141 cgggaggaga attggcggct atgacaatgg tcgatgctac cgttcgtctg attccagaag
     1201 ttataggtaa agaaacaagt catcaggatg attcattttc atcgggccta ttagaatatc
     1261 ctcagtatac gcgcccatat gattatttag gcatgacagt tcctgatgtt ctcatgagtg
     1321 gtcatcatga gaacatccga aaatggcgtt tagaacaaag tttgcgaaaa acactagaac
     1381 gtcgaccaga cttgttagaa aattatgcaa tgacggatga agagagactt atcttagaga
     1441 aaattaaaac agaaatagaa agaacagata cagtaaatga acaaaacaat ttatgatatc
     1501 actattgtag gaggcggacc agtaggtctc tttgctgctt tttatgctgg actacgcgga
     1561 gtatccgtta aaattattga aagcctttcg gaattaggag gccagccagc tatcctttat
     1621 cctgagaaaa aaatctatga tattcctggt tatccggtga ttactggacg tgaattaatt
     1681 gataaacata ttgaacaatt agagagattt aaagactcta ttgaaatctg tttaaaagaa
     1741 gaagttttat catttgaaaa agtagatgat gttttcacaa ttcaaacaga taaagatcaa
     1801 catttgtcac gtgctattgt ttttgcttgt ggcaacggtg cttttgctcc gcgtttacta
     1861 ggtttggaaa atgaagaaaa ttatgcagat aataatttat tctataacgt tacaaagtta
     1921 gaacagtttg caggtaaaca tgttgttatt tgtggtggag gcgactcagc tgttgattgg
     1981 gccaatgaat tagataagat tgcagctagt gtagcgattg ttcatcgtag agatgctttt
     2041 agagcgcatg agcatagtgt ggatatacta aaggcatcag gtgttagaat tttaacccct
     2101 tatgtcccaa ttggtttaaa tggagatagt cagagagtta gcagcttggt cgttcaaaaa
     2161 gttaaaggtg atgaagtaat tgaattacct ttagacaact tgattgttag ttttggcttt
     2221 tctacttcta ataaaaattt acgttattgg aatttggatt ataaacggtc tagtatcaat
     2281 gtttcttcac tttttgagac gactcaagaa ggcgtgtatg ctattggaga tgctgctaat
     2341 tatccaggaa aggtagaatt gattgcaaca ggatatgggg aagcaccagt cgctataaac
     2401 caagctatca attatattta tcctgatcga gataaccgtg tagtccattc aacatcattg
     2461 ataaaataac tataaataaa actgataagt ttataaaaaa ggattatata tattgttaaa
     2521 aaaataacaa aacttcctat ttattttgtg atataattaa ctttgtcgtt atgaatgata
     2581 ttaaataaat gaagcgaggt attttatgac aaatacggta acaccaaaag agacggctgg
     2641 ctcttttatc aataaagtgt taggtgggac agcaactgca atcgttgtag cactcatccc
     2701 aaatgctatc ctagccactt ttttgaaacc atttttatct tacggacttg ctgcagagtt
     2761 cttacacatc gtgcaggtat tccaattttt cacaccaatt atggcaggat ttttgattgg
     2821 tcaacagttt aaattcaccc caatgcaaca gcttgctgtt ggaggtgctg catatattgg
     2881 ttctggtgcg tgggcttaca cagaagttat tcaaaaagga gttgctactg gtagtttcca
     2941 attgcgtggg attggtgatc tcatcaatat gatgctaaca gcagctttag cagttttagc
     3001 agttaagtgg tttggtaata aatttggttc cctaaccatt atcttgttac ctattatcat
     3061 aggtacaggc gtaggctatt taggatggaa attactccct tacgtttcat atgtgacaac
     3121 acttattggt caaggaatta attcttttac tactttacaa ccaattgcga tgtcaattct
     3181 aattgcgatg gctttttcaa tgcttattgt aagtcctatt tcaacagtag cgattggtct
     3241 agcgattggt cttaatggta tgtcagcaag tgcagcttca atgggagttg cttcaacaac
     3301 agcagtgcta gtttgggcaa cgatgaaggc taataagtct ggagttccaa ttgctattgc
     3361 tttaggtgca atgaagatga tgatgccaaa tttcctaaag catccagtaa tggcgattcc
     3421 aatgcttatg actgctacag taagttctct aacagtccca ttgtttaaat tggtggggac
     3481 accagcttcg tctggttttg ggcttgttgg tgcagtaggt ccgattgctt catttgaagc
     3541 aggagcaagt atgttgattg ttatcttatc ttggctcgtt attccttttg cagtaggttt
     3601 tgtatcacac aaaatttgta aagacattct gaaactttat aaagacgata ttttcgtttt
     3661 tgaaggtcaa aattaatttg gaggttgtat gttagtttat attgctggtt caggcgctat
     3721 gggatgtcgt tttggatatc aaatttctaa aacaaatcat gatgttattt tacttgataa
     3781 ttgggcagac catattatgg ctattaaaga aaatggcttg aaggtaacag gtgatacaga
     3841 ggaccttgtt aaacttccta tcatgaagcc aactgatgcg acagaagaag cagatttaat
     3901 tattttgttt actaaagcaa tgcagttgcc aaatatgctt caagatatta aaaaaattat
     3961 tggtaagaaa acaaaggtct tatgtctttt aaatggatta ggacatgaag atgtcattcg
     4021 tcaatatatc cctgaacaca atattttgat gggagtaaca gtttggactg cagggttgaa
     4081 aggaccaggg catgctcatc tggaaggagt tggttctgta aatcttcaaa gtatcgatcc
     4141 taacaatcaa gaagcaggtc atcgcgttac tgaattgctt aatgaagcaa aattacaggc
     4201 tacttatgac gaaaatgttt tgccaaatat ttggagaaaa gcttgtgtta acggaacgat
     4261 gaattcaact tgcgccttgc tagattgtac aattggtcaa ctctttgcta gtgaagacgg
     4321 cgtaaatatg gtacatgaaa ttatccatga atttgttact gttggaaaag cagaaggtgt
     4381 agagcttgat gaagaagaaa taacaaagta cgttatggat acatctgtta aagcagccca
     4441 tcattaccct tcaatgcatc aagacttagt tcaaaatcag cgcttaactg aaattgattt
     4501 cttaaatggc gcagtgaata aaaaaggtga aaacctaggc attgatacac cttactgtcg
     4561 attaatcaca caattaattc acactaaaga aaatgtactt agcataaaat aataatcaac
     4621 cgggagaata gtgcccggtt ttttgatatg cttaatgata atgattttgt tatactaaga
     4681 ataataagag ttgggtattt ttttcgagaa gtatttacag tataaaatat tacatttgta
     4741 ttacaaatat ttctaaaata tgttgacatg ttacgaaaag gatattataa tagtagtgta
     4801 aataaataag gaagtaggtg tttatgatga aaaaaggaca agtaaatgat actaagcaat
     4861 cttactctct acgtaaatat aaatttggtt tagcatcagt aattttaggg tcattcataa
     4921 tggtcacaag tcctgttttt gcggatcaaa ctacatcggt tcaagttaat aatcagacag
     4981 gcactagtgt ggatgctaat aattcttcca atgagacaag tgcgtcaagt gtgattactt
     5041 ccaataatga tagtgttcaa gcgtctgata aagttgtaaa tagtcaaaat acggcaacaa
     5101 aggacattac tactccttta gtagagacaa agccaatggt ggaaaaaaca ttacctgaac
     5161 aagggaatta tgtttatagc aaagaaaccg aggtgaaaaa tacaccttca aaatcagccc
     5221 cagtagcttt ctatgcaaag aaaggtgata aagttttcta tgaccaagta tttaataaag
     5281 ataatgtgaa atggatttca tataagtctt ttggtggcgt acgtcgatac gcagctattg
     5341 agtcactaga tccatcagga ggttcagaga ctaaagcacc tactcctgta acaaattcag
     5401 gaagcaataa tcaagagaaa atagcaacgc aaggaaatta tacattttca cataaagtag
     5461 aagtaaaaaa tgaagctaag gtagcgagtc caactcaatt tacattggac aaaggagaca
     5521 gaatttttta cgaccaaata ctaactattg aaggaaatca gtggttatct tataaatcat
     5581 tcaatggtgt tcgtcgtttt gttttgctag gtaaagcatc ttcagtagaa aaaactgaag
     5641 ataaagaaaa agtgtctcct caaccacaag cccgtattac taaaactggt agactgacta
     5701 tttctaacga aacaactaca ggttttgata ttttaattac gaatattaaa gatgataacg
     5761 gtatcgctgc tgttaaggta ccggtttgga ctgaacaagg agggcaagat gatattaaat
     5821 ggtatacagc tgtaactact ggggatggca actacaaagt agctgtatca tttgctgacc
     5881 ataagaatga gaagggtctt tataatattc atttatacta ccaagaagct agtgggacac
     5941 ttgtaggtgt aacaggaact aaagtgacag tagctggaac taattcttct caagaaccta
     6001 ttgaaaatgg tttaccaaag actggtgttt ataatattat cggaagtact gaagtaaaaa
     6061 atgaagctaa aatatcaagt cagacccaat ttactttaga aaaaggtgac aaaataaatt
     6121 atgatcaagt attgacagca gatggttacc agtggatttc ttacaaatct tatagtggtg
     6181 ttcgtcgcta tattcctgtg aaaaagctaa ctacaagtag tgaaaaagcg aaagatgagg
     6241 cgactaaacc gactagttat cccaacttac ctaaaacagg tacctataca tttactaaaa
     6301 ctgtagatgt gaaaagtcaa cctaaagtat caagtccagt ggaatttaat tttcaaaagg
     6361 gtgaaaaaat acattatgat caagtgttag tagtagatgg tcatcagtgg atttcataca
     6421 agagttattc cggtattcgt cgctatattg aaatttaaag ctaaatcttt ttagatttag
     6481 ctttactaat attatgtgtg ttggaaaaaa cagtggagat ttacg
//
LOCUS       DATRNX010000103         6485 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRNX010000103 DATRNX010000000
VERSION     DATRNX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBC81_001664"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBC81_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6912688.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBC81_001665"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBC81_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6912689.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3369
                     /gene="nikA"
                     /locus_tag="VBC81_001666"
     CDS             1753..3369
                     /gene="nikA"
                     /locus_tag="VBC81_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6912690.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3356..4300
                     /locus_tag="VBC81_001667"
     CDS             3356..4300
                     /locus_tag="VBC81_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912691.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4300..5106
                     /locus_tag="VBC81_001668"
     CDS             4300..5106
                     /locus_tag="VBC81_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912692.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5094..5882
                     /locus_tag="VBC81_001669"
     CDS             5094..5882
                     /locus_tag="VBC81_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912693.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5869..6485
                     /locus_tag="VBC81_001670"
                     /pseudo
     CDS             5869..6485
                     /locus_tag="VBC81_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2140 a          984 c         1270 g         2091 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa
     2341 tgggttgtga aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac
     2401 tggggaaaga aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg
     2461 cgtgctttag cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt
     2521 ttagacactt ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct
     2581 atgtcaacaa gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt
     2641 cgtcaggcaa tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga
     2701 acagaaaagc cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta
     2761 aatccttata gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag
     2821 atgggaaaag ataaggttcg tgaaaaagat ggtaaagctc taaccttacg tcttccttat
     2881 atcgccacta aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa
     2941 atcggtatta atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa
     3001 aagggtaatt ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct
     3061 tggatgtcgg cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa
     3121 aatctagcaa ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag
     3181 gaagaaaatg ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta
     3241 tatatcccat taacttatca atcggtgatt tctgtttata ggaaaggtga ttttaaaacc
     3301 atgcgttttg cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta
     3361 tctaagtagt tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc
     3421 acttctaaca tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg
     3481 tttatcaaaa ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt
     3541 agataagcca ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt
     3601 tggatattct tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc
     3661 tactttattt ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg
     3721 tgtgtgggct ggcttacatg aatctgctcg gagtgatcac ttgattcgat ttttgagttt
     3781 ttctagtgtg tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc
     3841 aaaattaaat cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag
     3901 tattacctta agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag
     3961 tcaagaaaat agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta
     4021 tattgtcaca catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt
     4081 gacttgggtt tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg
     4141 aataggacgt ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg
     4201 tatgctaatt tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa
     4261 ttgggttgat cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt
     4321 tcgagtagct tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc
     4381 tttgatccac aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg
     4441 ttagggactg accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat
     4501 tcactgtttt tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt
     4561 cttattgttg gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt
     4621 attttagctt ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt
     4681 ctagggaatt taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg
     4741 accaatttag ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga
     4801 ctttcagtct ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc
     4861 gttatggtcc ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta
     4921 gggattggtg tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga
     4981 tattttagga cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta
     5041 ttttccttta atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac
     5101 agttagaaat aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta
     5161 gttgtaaaat agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa
     5221 ccctcttggc aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca
     5281 tattttttaa gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg
     5341 gacgagatat tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa
     5401 ttgaagcgca tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct
     5461 tatctaaagc gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa
     5521 aaaaataccc ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat
     5581 tatctcttga tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata
     5641 attgttctac tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta
     5701 ctgtaacgca tgattatcag ttagctagag acctgggggg acaattatta gttataagtg
     5761 agggagaagt tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact
     5821 atacgaaagc actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt
     5881 aatcatgtcg gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt
     5941 aaaagaggag aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc
     6001 cgactcatta taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt
     6061 tacacaccta aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg
     6121 gtcaatccat attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaac
     6181 aacttttgaa ttatgtcaaa tattagaagc ggtgggttta gatggaactt atctaaaata
     6241 taaagctaga caacttagtg gtggtcagct acagagagtt tgtatcgcta gagctttact
     6301 cttaaagcct aaaattatta tttttgatga atccttaagt ggattagacc cagtaactca
     6361 aattaaaatg ctacgtttat tacaaaaaat aaaacgccgt tatgaattaa gttttataat
     6421 gatttctcat gaccctaaaa tttgtcaagc tatctgtaac cgggtctttc tgataaaaaa
     6481 tggtt
//
LOCUS       DATRNX010000104         6478 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRNX010000104 DATRNX010000000
VERSION     DATRNX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1166
                     /locus_tag="VBC81_001671"
     CDS             <1..1166
                     /locus_tag="VBC81_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6912694.1"
                     /translation="HSLLGLTITVCDQNFSVIREYKSEKTISLFYNHYLILSNFSKTQ
                     HDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQINASEQDYFID
                     RLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFDLDKIKQFSKQ
                     NMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELRSEKNYSIMIY
                     DRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFYTQQVHSLKKH
                     LETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEKHITIQQYFLN
                     LKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQKPKTI"
     gene            complement(1361..1885)
                     /locus_tag="VBC81_001672"
     CDS             complement(1361..1885)
                     /locus_tag="VBC81_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6912695.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            2017..2880
                     /locus_tag="VBC81_001673"
     CDS             2017..2880
                     /locus_tag="VBC81_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6912696.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2965..3819
                     /locus_tag="VBC81_001674"
     CDS             2965..3819
                     /locus_tag="VBC81_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6912697.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3929..4956
                     /locus_tag="VBC81_001675"
                     /pseudo
     CDS             3929..4956
                     /locus_tag="VBC81_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4972..5361
                     /locus_tag="VBC81_001676"
     CDS             4972..5361
                     /locus_tag="VBC81_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6912698.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            5371..5766
                     /locus_tag="VBC81_001677"
     CDS             5371..5766
                     /locus_tag="VBC81_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6912699.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            5794..6111
                     /locus_tag="VBC81_001678"
                     /pseudo
     CDS             5794..6111
                     /locus_tag="VBC81_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            6198..>6478
                     /locus_tag="VBC81_001679"
     CDS             6198..>6478
                     /locus_tag="VBC81_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6912700.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWR"
BASE COUNT         2215 a         1057 c         1124 g         2082 t
ORIGIN      
        1 tacacagtct tctaggtcta actattactg tttgtgatca aaatttttct gttatcagag
       61 agtataaatc cgagaaaact atttcacttt tttacaatca ttaccttatt ttaagtaact
      121 ttagtaaaac tcaacatgat tttttatttc attatggctc tttaggagaa ctttttcttg
      181 tccaccatat tcaacaatat tatattatca tcggtccctg gcgtagcaat gttattgacc
      241 ctttacttct taagaaaaag ctgactgaaa cacaaatcaa tgctagtgaa caagactact
      301 ttattgatag actatcccaa ttacctttct tctcacttag tcaaatccga gaattactaa
      361 tagtaaccaa ttattgtcta actggggttg tgaaagataa attatcagag cccttacatt
      421 actacacgaa agggtggagc aactcctttg atctagataa aattaagcaa ttttctaaac
      481 agaatatgag ttcttacaaa tatcagtatc attttgaaaa taatattcta aaagctgtta
      541 aatcaggtag tgaatttctt ttgaaagaaa cagtggaaca atttagcaat tctatcgtac
      601 ctataatcag tggagatgaa ttgcgatctg aaaaaaacta ctccatcatg atttatgatc
      661 gtctctcaca ggctaccatc caagcaggac ttgatatcga aacagcttat cgagcacgag
      721 atcgttttat aaaagaaaac gagtcaacta taagtctaaa tgaagtttta aaattacgtg
      781 atactgctat cttattctat actcaacaag ttcattcttt aaaaaaacat ctcgaaaccc
      841 ctcattctca aactattgtc gcagtgattc gatatcttga aaataatcta aatcgcttta
      901 ttaaaacaga agaaatagct aaagaatgtc acatgagtga atcgaagtta cgaaaactat
      961 ttaagcaaga aaaacacatc actattcaac aatattttct aaacttaaaa atcgaagctg
     1021 ctaagcaatt actagatgaa aataagaaag tagaagaagt ttccaattta cttggatttt
     1081 ccacttcttc taatttttca aggacattta aaaaaatagt gggaattagc ccactagaat
     1141 ataagcaaaa gcctaagaca atatagtaat cgaaaaatat tcattttgct tatggtgaca
     1201 tcaacaatcc caaagaaact ctttcatcaa ggggactttt ttatctcccg aattatactc
     1261 atttaatcta ataatttcaa ctcgctatat tagacaatca atccattcat cacctctgat
     1321 aacgctaatg aaataagaga atctcccaac tctctataat ttacctctct accatagtta
     1381 aataatattt tagtaaatct tcaacagtca ttttctgtct ttgatgaagc caccacttta
     1441 aggtctcgat aaaactggat aaaagaaatt gttttaagaa atcttcagga atgtccacct
     1501 ttgtaatata ctcctcacgc aatctagggt aaacatcgtg ttctaattca cttctaaagc
     1561 gtaataaaaa atagggatcg tcagataata ataacgtagc gatactatct tgattttgtt
     1621 caaaatgttt taagatatga actaaatact cttcaaacgt aacatctcta ccctgcttaa
     1681 ataagtggtg aaaaaggtct tcacagagct ccttgagtaa cacttctttg ctttcatagt
     1741 gagagtaaaa agttgatctt cctacatttg caagtccgat tacatcttga accgttattt
     1801 tagaataatc attttcctgt agcaagctta taaaagcatt ataaatcgct acttttgttt
     1861 tctgaatttg tctatctttt accatatagg acactttcta agttttgttc agtattgaac
     1921 aagtgttctt ttttgtttct agcaattctc ttttatctcc tagataatag aagaaagaat
     1981 cacactacaa taccagtata acaaaaggag aaaaatatga catcaaacaa aaaagtggct
     2041 atcgctttta tattaaatat cagcttttcc gtccttgaat tcatctttgg aagtctattc
     2101 ttctcaggag ctatcttagc cgatgctgtt catgattttg gtgatgcaat agctattggc
     2161 atctcagcta ctttagaaaa aaaatctaag aaagatgaag atacgatatt ttcattaggt
     2221 tataaacgtt ttagcctttt aggagctctt atcacaagtc taattcttat aagtggatct
     2281 atcttagtca tgatagaaaa tattccaaaa ttgtggcatc ctactcctgt taattatcac
     2341 ggcatgttta ttctagctgt catcgctatt atcattaatg gtctagctag ttttattctc
     2401 cattctggtc aaagtaaaca tgaggaaata ttaagccttc attttttgga agatattcta
     2461 gggtggttag ctattattgt tatatctctt atcttaaatt ggaaaccgtt gtatatcctt
     2521 gatccattat tatcagttgc catatctact ttcatcttat ctaaagcact tcctaaatta
     2581 ctatcaaccc tgaaactttt tttagatggt gttccagatt ccatagatta cgctgcatta
     2641 catgatgaac ttaagggact ttcgcaagta agatctatca atcaactcaa tatctggtca
     2701 atggatggta ttgataatcg agcaatcatt cactgttgct taaatcaatt gatatctgaa
     2761 aaagattgta aaagagctat tcgaacgatt tgccaacatt ataagataaa cgatgttact
     2821 gttgaaatcg attattcttt aagagaacat caaaatcatt gcaaaccatt aaaaaactaa
     2881 cctgagtgtt agttttttcc ttgacttgga gttaactcta agtgttaaga ttaggtcaat
     2941 actaatgaaa gaaggaaaat aaagatgaca gatacaatga tattaaatga caatcataaa
     3001 ataccaactt taggtttagg tacgtggatg atagataatg atagggtagc tcaagttgtt
     3061 aaagatgcta tcgaattagg ctaccgccat attgatactg cacaagcata caacaatgaa
     3121 aaaggggtcg gtgtagggat taaagaatct ggtattacac gtgaatcttt atttgttacg
     3181 actaaaattg ccgctgaaca taaagattat gaaagtgcta caaaatcaat tgatcgatca
     3241 ctagaagctt tagggcttga ttatattgat ttaatgatta ttcatagtcc acagccttgg
     3301 aaggaatggc gtgaaacaga taaacatttt gaccaaggaa atttagaagc ttggagagct
     3361 ttagaggatg ctcaaaaatc tggtaaggta aaatccatcg gtgtctcaaa ctttttagaa
     3421 aaagacttag aaaatatctt aaaaaatggt tatgttaaac ctgctatcaa tcaaatctta
     3481 gctcacatcg gaaatacacc tttcgacttg attgattact gtcaaagtaa aggtattcaa
     3541 gttgaagctt attcaccaat tgcacatgga caagcattga aatctgatgg tattcaaaaa
     3601 atggctgaaa aatatggggt tagtgttgct caattatgta ttcaatattt actacaatta
     3661 aacctgatcg ttttaccaaa ggcttctagt aaagaacatt tacagtctaa tctagacttt
     3721 gactttgtaa ttagtgacga agatatgtcg atattaaaat cacttatgtt tgacgattat
     3781 ggggaatttt ctaacttccc tgtttttagt ggaaaatgaa accatatgcg gatttttaca
     3841 gatgtttaat tttcaaaatt tttaagtttt tcttgacttg aagttaactc taagagttat
     3901 aatgaagtta tagaaaagag gtaaggttat gaaagtagct acatttattg aacctggcaa
     3961 aatggttata actgatacac caaaaccagt cattgaacaa gagacagatg ctgttatcaa
     4021 aattgttaga gcctgtgttt gtggttcaga cttgtggtgg tatcgtggta tttcaaaacg
     4081 tgaaagtggt tcttttgcag gtcatgaggc tattggtatc gttgaggaag ttggtactaa
     4141 agtaactgac gtgtcaaaag gtgattttgt tattgttccc tttacacatg gctgtggcca
     4201 atgtccgtct tgtaaggctg gatttgatgg aaattgtaca aatcatcaag ctgaaaaaaa
     4261 tgtagatacc aaggtcaata tctacgttat actaatgcaa actgggcatt ggttaaaatt
     4321 cctggtcaac cttctgacta tgataatgag acacttaact ctcttctcac cttatcagat
     4381 gtaatggcaa ctggttatca tgctgcagaa gttaaagaag gtgatacggt agttgtcatg
     4441 ggagatggtg ctgttggact ctgtggtgtt attgctgcta aaatgttagg tgctaaccgt
     4501 atcattgcaa tgagtcgtca caaagatagg caggaactag cattgacttt tggtgcaacg
     4561 gatattgtcg aagaacgggg tgacgaagcc gttaaacgtg ttttagattt aaccaatcaa
     4621 gcaggtgccg atgctatttt agaatgcgtt ggtacagagc agtctgttga tacagctacc
     4681 caaattgcta ggcctggtgc tgtcatagga cgtgttggta tcccacaaaa tccagacatg
     4741 aataccaata atttattctg gaaaaatatc ggccttagag gtggcattgc ttctgtaaca
     4801 acatttgata agtctgtcct tttagatgct gttctaactc ataaaattaa cccaggctta
     4861 gtttttacaa aatcctttgt attagatgat attcaaaagg cttatgaagc aatggataaa
     4921 cgtgacgcta tcaaatcctt agtgattgtt gactgaatat cgatgttaaa aatgcatatc
     4981 aaagaagtta gtaaaaagac tggcattacg tcagatacca ttaggtatta tgagagaatt
     5041 aatcttattc cccctattca tcggagtgaa tcaggaattc gtgagttcac acaaaacgat
     5101 attgatattt tagaatttgt ccgctatttt agaggcgcag gtgtttccgt tgaaagtctc
     5161 attgattata ttcatttggt tgaaaaaggc gattccacaa tcccagctag gctagaaatt
     5221 ttaaaagata aacgcaacaa ccttgaagat aggcttcaag gcctccaaga agcacttaat
     5281 cgtctaaatc acaaaataga caattatcaa aaagttcttg ttcctagaga acaagaactt
     5341 tttgatccaa caacgaaatg aggaaatctt atgcaagaaa caactaagtc tatctttcct
     5401 aagggtgaaa aaaatccata tggtgaattc tttattaggc aatcctatct cgcagcatta
     5461 gcaaaatctc cagatgggaa tgtttcagtt ggaaatgtaa cctttgaagc tggctgtcgt
     5521 aataattggc atgttcattt ggacggttat caaatcctcc tcgttacaga aggttcaggt
     5581 tggtaccaag aagagaataa aaaggcagtt tcattagaac caggtaacgt gattgtaact
     5641 gataaaggcg ttaaacattg gcatggtgct aaaaaagatt ctgaatttgc acatattgcg
     5701 attacagctg gtaagagtga gttttatgaa gctgttagtc ttgaagaata ttcaagacta
     5761 ggttaaattc aagtaaggag gaatcaatcc attatgaaag aaaaacaaac agccggacgt
     5821 cgtcaattag aagaatttgc acctgaattt gccagatata atgatgatat tttatttggt
     5881 gaggtttggg caaaagagga tcatttaaca gataaaacac gttctattat tactatatct
     5941 gccttaatta gctgaggaaa tcttgaacag ttagagcatc atcttcaatt tgccaaacaa
     6001 aacggagtta ctaaagaaga aattgctgac attatcactc acctcgcttt ttatgttgga
     6061 tggccaaaag cttggtcagc ctttaataaa gctaaggaaa tttggatata aaataacaga
     6121 catagcaagg gagatacttt cccaaaagcc aaaaagaaag atcaaccaat tattaaacca
     6181 actattagga gaatgatatg cttactattc aagaaatgaa agatacaacc tttgaattac
     6241 aagaaaattt tagaaggtta aattatccta ttaaacaagt tgctaaagac ttgcaactta
     6301 atatcagtga agttgaatct cttttaagtc ttgatgttac ttatcctgga gatgtttgta
     6361 tgctgagaga ttatctagaa gatatgttga aaaaagaggg taaagaagtt tatccatttt
     6421 ctagattagc atctcattca gcaaatcgtt ggtaccctta tgagacacca tggcgtta
//
LOCUS       DATRNX010000105         6439 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRNX010000105 DATRNX010000000
VERSION     DATRNX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(26..1498)
                     /locus_tag="VBC81_001680"
     CDS             complement(26..1498)
                     /locus_tag="VBC81_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912701.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     IPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1729..2565)
                     /locus_tag="VBC81_001681"
     CDS             complement(1729..2565)
                     /locus_tag="VBC81_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6912702.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2565..3935)
                     /locus_tag="VBC81_001682"
     CDS             complement(2565..3935)
                     /locus_tag="VBC81_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6912703.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4033..5280)
                     /locus_tag="VBC81_001683"
                     /pseudo
     CDS             complement(4033..5280)
                     /locus_tag="VBC81_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            5536..>6439
                     /locus_tag="VBC81_001684"
     CDS             5536..>6439
                     /locus_tag="VBC81_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912704.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVGGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGR"
BASE COUNT         2189 a         1076 c         1190 g         1984 t
ORIGIN      
        1 gtaacctagt tctttttaga tgtctttatt taactccaga cattagtttt tcaatgcgtg
       61 gaacataaaa taagaggata aatccaaata caactgttat accaccaaca acaccataat
      121 aaagaatttg agtatcaggt gtgtacaatt taacgatttg agcgttaata gcttgtgctg
      181 cagcattact taagaaccag atgctcatca tttgagcttg gaatgcttta ggtgcgagct
      241 tactagttgc tgataatcca actggtgaga ttagcatttc cccaacgatg acaatagccc
      301 aactcattgt taaccaaaga gggcttactt tagcgttaac tccaaagagt aaacctggta
      361 gcatcatcca taagaatgaa gctccagcaa agaaaagtcc atacgcaaat tttttaggtg
      421 atgaaggttg cttacttccc catttagccc ataaccaagc aaagaatggt acataaagca
      481 taatgaagag agggttcata ctttggaaat aacttgatgg gaaattaata tgatgcccaa
      541 agaagttaag gtaaagtttt gtttgttcat ctgcaaataa ggctaagaca actgaacctt
      601 gttcttcaat tgaccaaaat agaattgagg cgataaagag agggatatat gcccacactc
      661 gagaacgctc aacagaagaa atctttttac tacttaaaat cttgataaaa taaataattg
      721 ggatggcgat tgcaataata ctaaaaatat cgataattac ttcaattgtt agcatatgca
      781 tcaagtgaag tagaataagt acaataacaa taccaataag gataagccca acttttttat
      841 atagagagga cttttcttca ggacttaaag gatcgtttgg tctgagactc tcttcagtca
      901 aatatttttt tccatctaag gtatattgga ggagaccaaa gaacatacca atagcagcaa
      961 gtgagaaacc aagatggaaa tttacttctt gtccgaggta ccccactaca atgggtgaaa
     1021 taaaagcacc aaggttaata ccaaaaacaa agatactaaa accagcatca cgacgagaat
     1081 cattttctcc atataaatta ccaaccatct ctgatacatt tggttttaat agtccagtac
     1141 caaggataat tagagcaatc gaaatgaata gagctgtttg accaaatggt atagctagag
     1201 caatatgacc tagcatgatt agaataccac catacagaac agttttacga ctacctagaa
     1261 tacggtcgct gacaaaacca ccaattactg atgagagata aaccagtgaa ccatagacag
     1321 ccatgattga tgcagcgaca gtcttgtcca taccaaggcc accttgagaa acactatagt
     1381 acatatagta cagcaaaata gcacgcatac catagtatga gaatctttcc cacatttcag
     1441 taaagaaaag agtggacaaa ccacgaggat gtccaaagaa tgattttgtt ttttccatga
     1501 aaaacgctcc ttttgtagat aacactatta ataatatagt aaatagaagc aactgtcaaa
     1561 ttacttagca ctatcaagtt atttttttgt agaggttaac acccttggta aaccaagttt
     1621 tttaactatc tagcttagaa aaaggcttga attcaataga acccaagcct tttttagaaa
     1681 agaaaccaca gtgaaataaa aatgtcgtgt ctttaaatta tctgaagatt attttacaga
     1741 tccacctgta ataccttcaa catagtattt ttgcatgaac atgaaaagta ttgtgattgg
     1801 aattgcaata ataacagatc ctgctgtaaa tgacatgaac cactggttga tggtatcttg
     1861 ttgcaacatt gaaaagagac caatagcgac tgtgtacttg ctcgtagcat cacctaaaat
     1921 aacttttgca aagataaagt ccatccaagg gcccataaaa gcgattaatg cagtataaac
     1981 gataatgggt tttgacaatg gtagggtgat tttaaggaaa atatccaaac gggtcgcccc
     2041 atcaatcata gcagactcat ccaaagagta tggaatagta tcaaagaaac cttttgcgat
     2101 gtagaaggtt aatgctgctc ctgcagaata gacaaatatt aaagcggtta atgtttggtc
     2161 tagatttaga gcttttagga tgtagtaaac tgcaatcatt gacatgaacc caggaaacat
     2221 attcaagact aaagctagct ttaggaaacc attacggtgt ttgaatttga ttctgctgag
     2281 agaataagcc attgctacag tgattaaagt tgatagtata caagtacatg tagcgacaaa
     2341 taatgtattt aagaaccatt gaccaaatgg gaatgtgttt tgggtgaaga gtttagcgta
     2401 gttgtctaat gtccaagttt taggaataaa gtagttgaca aaggcacttc cttcaccacg
     2461 gaagcttgtg agaacgaccc aaacaatggg aaagagccac ataattgata gtactatcaa
     2521 taaaatataa acaaaagtaa ggttaagacg ttttttctta ttcattattt agctgcccct
     2581 tccttgtaag aatttgttct tgtgtaagct agtaaactaa atactgctga aagaataaag
     2641 attaagatac caacaacaga tgctaagtta taatctgcag cagttactgt caaattgtat
     2701 agccaagtga caagtaaatc ggtgctacct gcttggtagt atgttgagtt tgttggtcca
     2761 ccttgagtga gtaggtagat aacgttgaag ttgttaatat ttccgataaa ttgttgaatt
     2821 aaagtaggag tcataatcaa caaaatttgt gggaaagtaa ttgattgaaa gacttggaac
     2881 ttattcgccc catcaatttc agcagcttca atttgttctg caggtaaatt catgatgata
     2941 ccagtagcaa caagcattgt tacaggaata ccaacccaca tattaacaaa gatgattgag
     3001 aatttcgccc agaccggatc agaaaggaat ggaaggggat gtgctgaaga aataagtccc
     3061 cattttatta acaaggcgtt aaccggtcca gcgtcactta agaggtttct catgattaag
     3121 agagagataa attgagggac agccatggta ataacaaaaa tagtacgcca catctttttg
     3181 aatttcaaac cttttgtatt aattagtaag gcaagaataa taccaaagaa gaaattcgtt
     3241 acagtagcga aaacagccca aattaatgtc caagatagaa cagggaagaa agtactggcc
     3301 atacgcccgc taaagatatt accgaagtta gcaaaaccaa cccagtcaaa taaacttttt
     3361 ggcggtaagt ggttatgatc atagttagta aatgccaaac aaatcatata aactaacgga
     3421 aggattgtaa atagaagcac ccctattaaa ggaatagaca ttaaagtcat atggaagcgt
     3481 ccatctgtca agcttttaat atcttttttg aaggatggaa tttctcttcc ttcttctttc
     3541 aaggtcaata aatgtgctgc gcttttaatg tttgaccagt aaatataggc aaatacgcaa
     3601 cagaagataa gcgaagctaa tccaaaaatt agcattaaca tagagttatc accttgtgtc
     3661 gcaacttgga ttttaatacc atccacagtt ttagttgtca taccttgttc ttgggtccct
     3721 aaggaaataa gaccttttac agctgggata acttggtaaa caaaggtaat taaaaacaga
     3781 atttcactga ttaaaaataa aagtcccttg acaatttgtt tatttttgag gttagcaagt
     3841 cccataatta agaatgatag tttagttgct aaatcacctt tttgaaagac ctcttttagg
     3901 gacataccat caagtgagat cagtttttta ttcataatgg tctcctttaa ttgttatttt
     3961 acaaaataag agatgggaag cactctcaat tgaaaggaag agtactcacc tcatctcatt
     4021 actctagacg tactattttg ttttagcgat gtctttatcg aattgtttta gacgtttaag
     4081 gtaatcgcta gatttgattt taccactgta agtatcgcta agaatagcag cactttctgt
     4141 ccagaatgtt gacatttgac tcaacttagg cataaccgtt gtatatttat ctgagctacc
     4201 catttcgata actgcttttg caagttcatc tttttggacg ctatcagaag attgaattga
     4261 tgagttagca ggaacgatat gacgtttttc gaattgaatt ttttgacttt cagcattagt
     4321 tagatatgca gcgagtttgt agctagctga gattcgttta gtgtttgaac cagcaggtgc
     4381 ttggttaacg gcataaagtt taacgcccaa gaatgctttt tgttgaactt ctttgtcacc
     4441 gattttcatt gttgggtaaa cagcaacacc gattttatct tcaccgacag cttttttagc
     4501 agcgtcgtaa tcccatggtc cactttcaaa agcatgaaca gaaccatcgc caaatttaga
     4561 cattgtattt tcagctgtca agttgacaaa accatcattt ttcttttgat ctgcaatcca
     4621 tttaaggaca gaaacacctg cttcattacc ccagttagtg cctttagcat cttcaccaga
     4681 tttaccaaat aaagtgtcgc ctacagaaag gaaaagagga cctgttacat atgagttagc
     4741 tgctttaagc tgttgaccga atttcccttt gcttgtaatt gtttcgtatg atttaacgtc
     4801 gtcagcagtt aactttgttt tattataata aagaacttga gattcaatac caaatgggaa
     4861 tgcataagtt ttccctttat attgtgcacc agtaagtgat tgtttagtgt cgtttttagc
     4921 aatttctttt gagtattact ctggaatttc ttggataaca ccagattcta ctaattgacc
     4981 aagttggtca tgtggaagtg agaatacatc ggctgccttg cttgggtctt tttttacgtt
     5041 ttcttgagct ttggagtcat tagactcaat catttttaca gtaacgcctt tgttttcctt
     5101 ctcgaatttt ttaacaattg ctttataaga cgcttttgaa tctgttggga cccaaagttt
     5161 gatagttgtt tttgaagcag catcaactga gttagagtga gtagcagcaa ttgctcctcc
     5221 tgcaactact gaaagagcag cagtactaac aagtaatttt ttccaagtat ttttttccat
     5281 tttggttctc ctcctagaga atattttaat tacacctata ttatgcaacc gtttgcaaaa
     5341 aaagtcaagt ctttttttaa aaaaaataag aaaaataaaa aaataatatc aattaatact
     5401 aaatgagaag attattttag tgagagtatt acatttgcag agaaggcaaa cgtttgtttt
     5461 tttcttagat tagagtataa tagacaaagg actataccaa aaggtataaa atgattagaa
     5521 tgaagaggtc gtaggatggt aactataaaa gatgttgctg ccaaagctgg tgttaatcca
     5581 tcgacagtaa gtcgtgtact aaaagataat gaatctattt caagtaagac gaaagagcgc
     5641 gtgaaaaaag ctatggaaga gctgggctat gttcctaatg ttgcagcaca aatgttggct
     5701 agtggtttaa cgcaaaatat tggagttatt tttcctccac tggttttacc agaccgctta
     5761 agtgaaccct ttttcatgga gattctttct acattaacta atgaagcaaa agagcatggg
     5821 tttacagttt ctattgcaac aagtatgtct tctgaggagt tgttagaaca agtaaaatta
     5881 atgcatctac aaaaacgagt ggatggtttt attgttctct attcggatca aaaagatcct
     5941 atcagagagt atttattaaa atataaactg ccctttgttg ttggtggtgc tgctaatggc
     6001 tatgaaaatg agattactta tattgataat gataatcagc tgatggggaa atctgccatt
     6061 gattatctct ataaaaaagg acataaaagc atccagtttg ttacagatga tttaaattca
     6121 gaagtatcag aagagcgcta tttagggtat tttaaaggtg ctagaaagtt aggtttaaac
     6181 caaaagccag ctcttttatt tgacagaggc aatccgcaag ttttagagga atttataaat
     6241 agagtcaaag aagaagaaac aactgcttta attgttattg gtgatactgt ctctgttaga
     6301 gtaatgcaat ttctttcttt ctataaatta aaagttccag atgacatttc tatcatgacc
     6361 tttaataatt ctctcttctc acatctgatt catccttacc taagcacatt tgatattaat
     6421 gttaataatc taggaagga
//
LOCUS       DATRNX010000106         6427 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRNX010000106 DATRNX010000000
VERSION     DATRNX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..377
                     /gene="essA"
                     /locus_tag="VBC81_001685"
     CDS             <1..377
                     /gene="essA"
                     /locus_tag="VBC81_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6912705.1"
                     /translation="DTKRLESDSQKNSGFQTELTKDLFKKEDLKKLDKNRQSKVSLQK
                     KQSKQLFQYSNKGQSDRKKRIKQLFSEESNQFKFVTRTEEATVSADIGHMIMYLVLIS
                     LVLILASIMTFKIKDKKDEYTY"
     gene            361..603
                     /locus_tag="VBC81_001686"
     CDS             361..603
                     /locus_tag="VBC81_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6912706.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            603..1877
                     /gene="essB"
                     /locus_tag="VBC81_001687"
     CDS             603..1877
                     /gene="essB"
                     /locus_tag="VBC81_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6912707.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYYAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGCGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1884..6311
                     /gene="essC"
                     /locus_tag="VBC81_001688"
     CDS             1884..6311
                     /gene="essC"
                     /locus_tag="VBC81_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO6912708.1"
                     /translation="MLVICYYQSLRYEFNLEEEKSFLISSNGKSPIPVSDLENDITLK
                     NMQGQLVYIIDQKEKELTNGVEISGIVFYLANNQKEIYTPLDYEDILIGDKEGYHVRF
                     KEGAPNLLLKKIESNWQLNLFEGDIYLNNHLQKVVQQLPLSLGDEISFQGTIVKLFPD
                     EIQIWGGTDYETSLTKKVMSAYQFYEGYPDFHRSPRIIYRSSEDKITVNAPGNKPNKS
                     KDELLKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMIFSITGYFKNRKQYKQ
                     DLQERIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQEMADTYHHRIYEKTPL
                     HFDFLYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYFNNRYIKNMPIVANLS
                     HGPVGYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEEMDKWSWMRWLPHATL
                     QDVNVRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTLFSPHYVVIVTDEKLI
                     LDHVIMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDRNTGQLVIEEGELKET
                     DFELDHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMYQAEEFEDLHVQERWI
                     SHAPYKSLAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSGKSEIIQSYILSLAVN
                     FHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRLF
                     AKADVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPEFMKELVSTARIGRSL
                     GIHLILATQKPSGVVDDQIWSNSRFKLALKVADRGDSMEMLHTPDAAEITQAGRAYLQ
                     VGNNEVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYEILNDDLSGLDQAENI
                     KEVPTELDAIVENIQTLTKEMGISDLPQPWLPPLSNQIAVTDLRKEESVDLWSKAPSY
                     KVVLGFMDIPSQQAQEVAYHDFEDDGHLSIFAGPSMGKSTALQTVTLDLARHNSPEFL
                     NLYLFDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFISRIKTEMSDRKKALSRYNVAT
                     AKLYRQVSGETMPQILIVIDSYEGLREAQTLTNLEACFQNISRDGSSLGISLVISAGR
                     MAALRSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGRGLIKRDDIEVLQVAL
                     PTEGTETFDIINNIQNESDAMNSKWTGPRPKAIPIVPETLTFEEFKAYEDVKKASETN
                     QISLGISLKEVDNCSIPLSKFKQLLFTSDNPDNLSNLTRFLVKSVSLIKDSKVYLLDT
                     ASDYDDLTEQQIEYINNRSEIAGHLEAIVSEIYLREESGKIEPWFVIIPDMNSFVAET
                     NMDKEQILFLLDSAHKYHIYFLFGGLASYLMTNISDVPKAIRTQAQYVLLGMRVMDQS
                     VLPKTYNSKEPYLKPDMIYIHDRRQETMLKITQEIEMEH"
BASE COUNT         2199 a         1131 c         1162 g         1935 t
ORIGIN      
        1 ttgatacaaa acgtttagaa tcagattcac aaaaaaattc tggttttcaa actgaattaa
       61 ctaaagattt atttaaaaaa gaagacctta aaaaattgga taaaaaccgt caatctaaag
      121 tctcactcca gaaaaagcag tctaagcaac tgtttcaata ttcaaataaa ggacaatctg
      181 ataggaaaaa acgtatcaaa caattatttt ctgaagaaag taatcaattt aaatttgtta
      241 caagaacaga agaagcgact gtttcggctg atattggtca tatgattatg tacctggttc
      301 tcattagttt ggtactaata ttagcttcta taatgacttt taaaataaag gacaaaaagg
      361 atgaatacac atattaacat tactctagat atgggggaaa cctcaaaaga tttacgtatc
      421 cctttgaaaa ttagcgttaa gcaattagta atggaattag atagtatttt tagccgtact
      481 gaaaggcgta ctaaatatca actaagagtt ataaataaag gtattttatt gggagagaac
      541 gatatattag cgcaccatcc agtaacaact ggtgatcgta taaggataga ggagttttat
      601 taatggaaac agattcgttt gtatttgaag aacaagactt tcaatttgaa aaaggaccag
      661 atttttggca gttatctctt aagcgttcaa aagtaggaac tcaaaatctg caacaactgc
      721 aattgttaga aattcaccat tctttgttga tgcctatgac aactgcaatt aatgcagata
      781 ccattcaatt tcaattccag acagaagcgc acgctctatc ttttgaaagc tttaaaaaag
      841 aaactttatc tgaaaaatta cgattagcct tgaatgtctt ggatttagat aaggctttgt
      901 ctttgccagt aaatttcatt ttgcatccct ctaatctatt tttaacaaaa aatgcaactg
      961 ctaaaatagc ttatcgtagt cttcctggga ttatgacacc tgagaagttt ggtccagagg
     1021 agtttttata tcaattcaaa tgttttgtct ttgcattact gacgcaacat gactatatag
     1081 aattgtataa tggtgctatt tctgttattg aagtatcaga tttcctaaaa agcatttatt
     1141 atgcagaaac tattcaggct gttagagaca tcattactac tgattatgag cagcaagtag
     1201 aagtagaaac tcatacgtta gcaaaagtat caaaggcaaa atataagctt tataaataca
     1261 taagtgtctg gctaggagct ttatctacaa tactcttgat tccgctagta tatctggttt
     1321 ttattcacaa tccattcaaa gaaaaaatgt tggcagcgga tacttcattt attaaagttg
     1381 attataatca agtgattaat cgactagaac atgtaaaagt aagtaagtta ccttatacac
     1441 agaagtacga gttggcctat tcctatatta atggaatgtc attttctgaa gaacagcgtg
     1501 aagttatttt aaacaatgtt acgctcaaga ctgatgagct ctatcttgat tattggatta
     1561 atattggctg tggtttagat gatgatgcta ttgatgctgc caaacgttta gacgattctg
     1621 accttgttat ttatgctatt gttcagaaaa tggatcaggt tagaaaagac aatagtttat
     1681 ctggtaaaga tcgtgaacaa aaactttctg agctacaaac agactatgat aaatattgga
     1741 aagaccgtaa gacagcctta acagatgagg aatccaaatc gaaaaatagc aataatcatt
     1801 cgacaaattc caacaaagag tcatctgaat catcaagtac tacagcaagt acatcttcta
     1861 aaactaaaag taggtaggtg gatatgttag ttatttgtta ttatcaatca ctccgctacg
     1921 aatttaatct tgaagaggag aaatcctttc taatatcatc aaacggcaag tcgccaatac
     1981 cagtatcaga tttagaaaat gacatcacgc taaaaaatat gcaaggtcaa ttggtatata
     2041 ttattgacca aaaagaaaaa gagttaacta atggtgtaga gatttcagga attgtcttct
     2101 atttagcgaa taatcaaaaa gaaatctata ctcccttaga ttacgaagat atattaatcg
     2161 gtgataaaga agggtatcat gtccgtttta aagaaggagc ccctaattta ttactgaaaa
     2221 aaatagaaag caattggcag cttaatttat ttgaaggtga tatttatctg aataatcatc
     2281 ttcaaaaagt agtgcagcag ttacctttaa gtttaggtga tgagattagc ttccaaggaa
     2341 caattgttaa gcttttccca gatgaaattc aaatctgggg ggggactgat tatgaaacat
     2401 cattaactaa gaaagttatg tcagcttatc aattttacga aggctatcct gattttcacc
     2461 gctcacctcg tattatttat cgttccagtg aagataaaat tacagttaat gcgcctggta
     2521 ataaacctaa taagtctaaa gatgaactgt taaaattaat tgttcctcct ctagtgatga
     2581 ttggagtcag tattcttatt tctatttttc gtcctcgtgg tatctatatc attgcaacaa
     2641 tgtcaatggc tctagtgacg atgattttct ctatcactgg ttactttaaa aatcgtaaac
     2701 agtataagca ggacttacaa gaacgcattg attcctatca tgactattta tctgataagt
     2761 caattgaact tcagaagtta gctaaagagc aaaagcgtgg gcaacactat cattacccaa
     2821 cgattgaagg tttacaggaa atggctgata cttatcacca tcgtatttat gaaaagacac
     2881 cacttcattt tgacttcttg tattatcgcc taggtttggg agaagtacca acgagctata
     2941 atattcatta ttctcaacca gaacgttctg ggaaaaaaga tcctttagag aacgaaggct
     3001 ataacctcta ctttaacaat cgttatatta aaaatatgcc gattgttgcg aatttatcac
     3061 atggaccagt tggctatata ggaccacgag gtttagtatt ggagcaactc caattgatgg
     3121 ttaatcaatt agcttttttc cattcttacc atgatgttca atttattact attgttccag
     3181 aggaagagat ggataagtgg tcttggatgc gttggttgcc acatgcaacc ttacaagatg
     3241 tcaatgttcg tggctttgtt tataatcaaa gaagccgtga tcaagtgctc aacagtctta
     3301 accaaatttt aaaattacgt cgcacacaac gcgaagataa atctgccaaa gaagggactt
     3361 tattttctcc gcattatgtc gttattgtaa ctgatgaaaa gttaatttta gatcacgtca
     3421 tcatggaatt ttttacggaa gatcctacag aattgggatg ttctctcatc tttgttcaag
     3481 atgttatgag ttcactttca gaaaatatca aaaccattat taatattaaa gaccgtaata
     3541 ctggtcagtt agttatagaa gaaggagagc ttaaagaaac agattttgaa ttagatcatt
     3601 tcttagagga ttatgacaag gaaaatattt cacgtcgcct agctccgtta aatcacctac
     3661 aaaatcttaa atcttcaata ccagaagcag ttacttttat ggaaatgtat caagcagaag
     3721 aatttgaaga tttgcatgtt caagagcgtt ggataagcca tgctccgtat aaaagcttag
     3781 ctgttccact tggcttacgt ggtcaggatg atattgttta tctaaaccta catgaaaaag
     3841 ctcatggtcc tcatggatta gttgcaggaa caacgggatc agggaagtca gaaattatcc
     3901 agtcctatat cctttcttta gctgttaatt tccatccaca cgatgtagct ttccttctca
     3961 tagactacaa aggtggggga atggcaaatc tctttaagga cctgccacac ttacttggca
     4021 ccatcaccaa cttagatggt gcccaatcga tgcgtgcttt ggtttctatt aatgctgaat
     4081 tgaaacgtcg tcaacgttta tttgctaaag ctgatgttaa tcatattaac cagtatcaga
     4141 agaaatacaa acttggagaa gtttcagaac caatgcctca tctcttctta atctccgatg
     4201 agtttgccga attgaaatcc aaccaacctg agtttatgaa agagcttgtc tcaacagcgc
     4261 gaattggtcg ttccctaggg attcacttaa tcctagcgac ccaaaaacca tctggcgtgg
     4321 tagatgacca aatctggtca aactcacgct tcaaactagc tcttaaagta gcagaccgcg
     4381 gcgactccat ggaaatgctc cataccccag atgccgccga gattacacaa gcaggacgtg
     4441 cctacctcca agttggtaat aatgaagtct atgaactctt ccaatcagca tggtcaggag
     4501 cagattacca acctgaaaaa gacgaccaag ggattgaaga ccataccatt tattccatca
     4561 acgaccttgg ccaatatgaa atcctaaatg atgatctttc aggcttagac caagccgaaa
     4621 atattaaaga agtacctacg gaattagatg ccatcgttga aaacattcaa actttaacca
     4681 aagaaatggg catctcagac ttacctcaac cgtggctacc gcctctaagc aatcagattg
     4741 cagtaaccga tctccgtaag gaagaatcag tagatctctg gagtaaagcc ccatcttaca
     4801 aagttgttct gggctttatg gatataccaa gccaacaagc acaagaagta gcttaccacg
     4861 attttgaaga tgatgggcat ttgtcaattt ttgcaggtcc aagtatgggg aaatccacag
     4921 cccttcaaac tgtaacattg gacttagcac gtcacaatag tccagaattt ttaaaccttt
     4981 atctctttga ttttggtacc aatggcctct taccattaag acgtttgcca catgtagcag
     5041 atttctttac cattgatgat gacgaaaaaa tagccaagtt tatcagcaga atcaaaacgg
     5101 aaatgtctga ccgtaaaaaa gccctcagtc gctacaatgt tgcaacagca aaactttacc
     5161 gccaggtatc tggggaaacc atgcctcaaa tcctgattgt tattgacagt tatgaagggc
     5221 tacgggaagc tcaaacactg actaacttag aagcttgttt ccaaaatatt tcgcgtgatg
     5281 ggtcgtcact tggcatttcc ttagttatct cagctggtcg tatggcagct ttgaggtcgt
     5341 ccttaatggc aaaccttaaa gaacgtatcg ctcttaaatt aacagatgac tctgaatcac
     5401 ggacacttgt cggtcgtcac caacacatca tggaagatat tccaggacgc ggattaatca
     5461 aacgtgatga cattgaagtg ttacaagtgg ctctcccaac agaaggcact gaaacctttg
     5521 acatcatcaa taacatccaa aacgaatctg acgccatgaa cagcaaatgg actggaccaa
     5581 gaccaaaagc aataccaatt gttccagaaa cacttacctt tgaggagttt aaggcatatg
     5641 aggatgttaa aaaagcaagt gagactaatc agatttcact tggtattagc ttaaaagaag
     5701 ttgataattg tagcattcca ctttcgaaat tcaaacagtt actttttact tctgacaatc
     5761 cagataattt gtctaacttg acgcgcttct tagttaaatc tgtttcattg attaaagata
     5821 gtaaggttta tcttttagac actgcttctg attatgatga tttgacagaa caacaaattg
     5881 aatatatcaa taatcgaagt gaaatagcag gacatttgga agctattgtt tcagaaatct
     5941 atttaagaga agaatctggt aaaatagaac catggtttgt tattattcct gatatgaata
     6001 gttttgtggc agaaacaaat atggataaag aacagatttt attcttactt gactcggctc
     6061 acaaatatca tatttatttc ttatttggtg gcttagcatc ataccttatg actaatatct
     6121 cagatgttcc aaaagctatt agaacacagg cacagtatgt tctcttggga atgagagtta
     6181 tggatcagtc ggtattaccg aaaacatata attcaaaaga accttatttg aaaccagata
     6241 tgatttatat tcatgataga agacaagaga caatgcttaa aatcactcaa gaaatagaaa
     6301 tggagcattg agtatatgaa aaaacaaaga ctattactgc tttttggagg cttattaata
     6361 atgataatga tgacagcatg taaggattca aaaatcccag aaaaccgcac gaaaaaggaa
     6421 taccagg
//
LOCUS       DATRNX010000107         6417 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRNX010000107 DATRNX010000000
VERSION     DATRNX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..97)
                     /locus_tag="VBC81_001689"
     CDS             complement(<1..97)
                     /locus_tag="VBC81_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6912709.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNL"
     gene            300..2339
                     /locus_tag="VBC81_001690"
     CDS             300..2339
                     /locus_tag="VBC81_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6912710.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSSYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2449..3027
                     /locus_tag="VBC81_001691"
     CDS             2449..3027
                     /locus_tag="VBC81_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6912711.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(3061..4302)
                     /locus_tag="VBC81_001692"
     CDS             complement(3061..4302)
                     /locus_tag="VBC81_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6912712.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            4480..4608
                     /locus_tag="VBC81_001693"
     CDS             4480..4608
                     /locus_tag="VBC81_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6912713.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            4669..5982
                     /gene="obgE"
                     /locus_tag="VBC81_001694"
     CDS             4669..5982
                     /gene="obgE"
                     /locus_tag="VBC81_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6912714.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            6008..6166
                     /locus_tag="VBC81_001695"
     CDS             6008..6166
                     /locus_tag="VBC81_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912715.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         2134 a         1062 c         1277 g         1944 t
ORIGIN      
        1 gtagattaag cccgatggct ttaaaggttt ctcctctatc caaaccagat tggatgtcat
       61 tacggtctga gagagttagg tgtttgtgtt ttgtcatgat ggataaactc atttctaacc
      121 caaacgtctc agacttaatt ccaccataaa aatccttttc acactaactt ccggttttgg
      181 agggcagacg gaacttactt tgagaaatta cttaatataa aataacagtt aatattttag
      241 caatttgata acacttagat tataatgtaa acgtatactt ttttagattg gaagatgaaa
      301 tgaaacgtaa atactttgtt cttaatacgg tgacggtttt aacgttagct actgcaatga
      361 atactagcag tagctatgct aatagtactg agacaagtgc ttcagtagct cctactacaa
      421 atactatcgt tcaaactaat gacagtaatc ctaccgcaaa atttgcatca gaatcaggac
      481 aatctgtaat aggtcaagta aaaccagcta attctgcggc gcttacaaca gttgacacgc
      541 ctcatatttc agctccagat gctttaaaaa caactcaatc aagtcctgtc gttgagagtc
      601 cttctactaa gttaactgaa gagacataca aacaaaaaga tggtcaagat ttagccaaca
      661 tggtgagaag tggtcaagtt actagtgagg aactcgtcaa tatggcatac gatattatcg
      721 ctaaagaaaa cccatcttta aatgcagtca ttactactag acgccaagaa gccattgaag
      781 aggctagaaa acttaaagat actaatcagc cgtttttagg tgttcccttg ttagtcaagg
      841 ggttagggca cagtattaaa ggtggtgaaa ccaataatgg cttgatctat gcagatggaa
      901 aaattagcac atttgacagt agctatgtca aaaaatataa agatttagga tttattattt
      961 taggacaaac gaattttcca gagtatgggt ggcgtaatat aacagactct aaattatacg
     1021 gtccaacgca taatccttgg aatcttgctc ataacgctgg tggctcttct ggtggaagtg
     1081 cagcagctat tgctagcgga atgacgccaa ttgctagcgg cagtgatgct ggtggttcta
     1141 tccgtattcc atcttcttgg acgggcttag taggtttaaa accaacaaga ggattggtga
     1201 gtaatgaaaa gccagattcg tatagtacag cagttcattt tccattaact aagtcatcta
     1261 gagacgcaga aacattgtta acttacctaa agaaaagcga tcaaacgcta gtatcagtta
     1321 atgatttaaa atctttacca attgcttata ctttgaaatc accaatggga acagaagtta
     1381 gtcaagatgc taaaaatgct attatggaca acgtcacatt cttaagaaaa caaggattca
     1441 aagtgacaga gatagattta ccaattgatg gtagagcatt aatgcgtgat tattcaacct
     1501 tggctattgg catgggagga gctttttcaa caattgaaaa agacttaaaa aaacatggtt
     1561 ttactaaaga agacgttgat cccattactt gggcagttca tgttatttat caaaattcag
     1621 ataaggctga acttaagaaa tctattgtgg aagcccaaaa acatatggat gattatcgta
     1681 aggcaatgga gaagcttcac aagcaatttc ctattttctt atcgccaacg accgcaagtt
     1741 tagcccctct aaatacagat ccatatgtaa cagagaaaga taaaagagcg atttataata
     1801 tggaaaactt gagccaagaa gaaagaattg ctctctttaa tcgccagtgg gagcctatgt
     1861 tgcgtagaac accttttaca ccaattgcta atatgacagg actcccagct atcagtatcc
     1921 cgacttactt atctgagtct ggtttaccca tagggacgat gttaatggca ggtgcaaact
     1981 atgatatggt attaattaaa tttgcaactt tctttgaaaa acatcatggt tttaatgtta
     2041 aatggcaaag aataatagat aaagaagtga aaccatctgc tgacctaata cagcctacta
     2101 actccctctt taaagctcat tcatcattag taaatttaga agaaaattca caagttactc
     2161 aagtatctat ctctaaaaaa tggatgaaat cgtctgttaa aaataaacca tccgtaatgg
     2221 catatcaaaa agcacttcct aaaacaggtg atacagaatc aagtctatct ccagttttag
     2281 tagtaaccct tttattagct tgttttagct ttgtaacaaa aaagaatcag aaaagttgat
     2341 tgtaatgtaa aataattcct atttttcact ttttagtatc atattttcga tttttatgtt
     2401 gtaataaatt ataatgaaga tgtaaggaat tataaaatag gaaataatat gaaagtgaaa
     2461 aataagattt taacgatggt agcacttact gtcttaacat gtgctactta ttcatcaatc
     2521 ggttatgctg atacaagtga taagaatact gacacgagtg tcgtgactac gaccttatct
     2581 gaggagaaaa gatcagatga actagaccag tctagtactg gttcttcttc tgaaaatgaa
     2641 tcaagttcat caagtgaacc agaaacaaat ccctcaacta atccacctac aacagaacca
     2701 tcgcaaccct cacctagtga agagaacaag cctgatggga gcacgaagac agaaattggc
     2761 aataataagg atatttctag tggaacaaaa gtattaattt cagaagatag tattaagaat
     2821 tttagtaaag caagtagtga tcaagaagaa gtggatcgcg atgaatcatc atcttcaaaa
     2881 gcaaatgatg agaaaaaagg ccacagtaag cctaaaaagg aacttcctaa aacaggagat
     2941 agccactcag atactgtaat agcatctacg ggagggatta ttctgttatc attaagtctt
     3001 tacaataaga aaatgaaact ttattaaata taaaaaggag gactaacctc ctttttatat
     3061 ttaaatagcc cattcgccat ttctaaagat tgggacagca gttccatctg cacgaatacc
     3121 atcaatatcc atttgttctg aaccaatcat aaaatcaaca tgtgttgaag aacgatttaa
     3181 accagcttca tccaattctt gacttgtcat ttctgttcct ccttctacat tgaaggcata
     3241 tgcagatcca atagcgaggt gattagaggc attttcatcg aataaagtat taaagaaaat
     3301 tagtccagac agtgaaattg gtgttttatg tgggacaagt gcaacttccc caagcgatct
     3361 tgccccatca ttttcctcga tcaagcgttg gattgtttct tgcccttttt ctgcagtgac
     3421 attgataatt ttaccgtctt taaaagtaaa cgtcatattt tcgataataa cgccagcata
     3481 actgagaggt tttgtacttg tcacataccc atctgcacga cggtaatcag gtgctgaaaa
     3541 gatttcttca gtaggcatat tagcgataaa agtctctccc tgagcgttga gactaccggc
     3601 tgcctcccaa atgtgatttt taggcatacc aagcgttaaa tctgtacctg gtgcggtata
     3661 atgaagagca tcaaattgtt cttgatttag tctagtagct ttttctaata atttagcttc
     3721 atgtgcatcc caagcagcaa taggattatc ttcataaata cgatttaatt tgaaaatggt
     3781 atcccaaaga gcatcaactt gttggtcgct tgtttttaat tctgggaaga caagtgcagc
     3841 ccattctcta ccagcagctg cagccacgtt ccaagatact ttattagctt gtattgctct
     3901 actttgttcc tctaaggcaa tagcggttgc tctagttgtt ttagataaac gtttaggatc
     3961 aacagtagcg aaagcgttag ggttagaaga tttcacaact aatcgactag ccttcttatt
     4021 taatagcgct aaagatttat caacggtata ctgtggaaca ttcgtgagaa cttcgtctga
     4081 agcatgaagt agtcgctggc gcgtgataaa atcatctgta taatcaacaa taacttcact
     4141 tgctcccttt tcataagcga cttcagttaa aagccttgct aagtggactt gttccacatc
     4201 gattgttaaa gcgagagtgt gccctttttg gacatttaaa cctttagaaa taattaattg
     4261 ggcatatttt tttaaaaggt tgtcgaaatc ttgtaaaacc atactagctc ctttaaattt
     4321 actatttttt ataatttatc ataaactctt ataaagcaca aggaaatatg gaagcgattt
     4381 aataaagata tatttaagct aacggtttgt agtttgcgaa ataagacgtt tcgtgttaat
     4441 atagacttaa ctatatattc agtaaatgag gtgacatgag tggcttttgg agataacgga
     4501 caacgaaaaa aaactgggtt tgaaaaacta actctttttg ttgttatttt gatggtacta
     4561 gtaacagttg gtggtttggt atttggtgct attagtgcaa tcatgtaatg agaaacgctg
     4621 taaagcgttt ttttatactg aatagaataa agaataggaa ttttagaaat gagtatgttt
     4681 ttagatactg ccaaaattag tgttaaggca ggtcgcggtg gtgatggcat ggttgctttt
     4741 cgccgtgaga aatatgtccc caatggaggc ccgtggggcg gtgatggcgg taaaggcgga
     4801 tcggtcattt ttaaagtaaa cgaaggttta cgaactttga tggattttcg atataaccgt
     4861 aattttaaag caaaagctgg tgaaaaaggg atgactaaag gtatgcacgg tcgaggtgca
     4921 gaagacctta ttgtgtcatt accacctgga acaactgttc gtgatgctaa tacaggtaag
     4981 gttattaccg accttgtgga gcatgatcag gaattcgttg tagccagagg tggccgcggt
     5041 ggtcgtggaa atattcgctt tgcaacccca cgtaatccag ctcctgaaat tgctgaaaat
     5101 ggggagcctg gcgaagaacg tgaattacaa ttagaattaa aaattttggc tgatgttggt
     5161 ctcgttggtt tcccatcagt tgggaagtcg acgctactta gtgttgtttc agcagcaaag
     5221 cctaaaattg gtgcctatca ttttacgaca attgtcccta atttagggat ggttcgtaca
     5281 aaatcaggtg atagctttgc aatggctgat ctccctggtt tgattgaagg ggctagtcaa
     5341 ggtgtcggat taggaactca gttccttaga catattgagc gtacacgtgt tattctacat
     5401 gttattgata tgtcggcaag tgaaggacga gacccttacg atgactatat ttcaattaat
     5461 aatgagttgg aaacttacaa tcttcgattg atggaacgtc cgcaaattat tgttgccaat
     5521 aaaatggata tgcctgattc agaagagaat ttggcagcat tcaaagaaaa attagctgca
     5581 aactatgatg agtttgatga catgcctatg atttttccaa tttcaagttt agcacatcaa
     5641 ggactagaga accttatgga tgcgacggct aaattattag ccaatacaga agagtttctt
     5701 ctttatgacg aaactgacat gcaagaagat gaagcatact atggatttaa cgaagatgaa
     5761 cgtccatttg agattacacg tgatgatgat gcaacgtggg tattgtatgg tgataagcta
     5821 gaaaaactct ttgttatgac aaatatggaa cgcgatgaat caataatgaa atttgctcgt
     5881 cagttacgtg gtatgggcgt tgatgaagca cttcgagagc gcggagctaa ggatggagat
     5941 attgttcgta taggtaactt cgaatttgaa tttgtagatt aatgcagtac ttgattggag
     6001 gttttttatg ggagataaac cgatatcatt tcgtgataaa gatgggaact ttgtttcagc
     6061 ggccgatgtc tggaatgctg aaaaattaga agaactcttt aacacattaa atcctaatcg
     6121 taaacttcgt ttagaacgcg aaaaattagc aaaagaaaaa ggatagttaa gtaactatcc
     6181 tttttagtta ttttgggtat gaaaaaagac tctaaaacat aattgataga gtgttaaggt
     6241 acagattact ttattatttt aaacgttttt gagctttttg atatactgaa gagcattttt
     6301 tacaatggtt ggagtataag taataaattt ataagcaaaa taaaaagcac aaactaaagt
     6361 tataaaaaat agtaagtaaa taatagggtt ttgcgttaag aatccttgaa aaagaag
//
LOCUS       DATRNX010000108         6407 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRNX010000108 DATRNX010000000
VERSION     DATRNX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            184..1848
                     /locus_tag="VBC81_001696"
     CDS             184..1848
                     /locus_tag="VBC81_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6912716.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            1937..2860
                     /locus_tag="VBC81_001697"
     CDS             1937..2860
                     /locus_tag="VBC81_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6912717.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRNVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            2862..3779
                     /gene="srtC1"
                     /locus_tag="VBC81_001698"
     CDS             2862..3779
                     /gene="srtC1"
                     /locus_tag="VBC81_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6912718.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            3736..4587
                     /gene="srtC2"
                     /locus_tag="VBC81_001699"
     CDS             3736..4587
                     /gene="srtC2"
                     /locus_tag="VBC81_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6912719.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            4669..>6407
                     /locus_tag="VBC81_001700"
     CDS             4669..>6407
                     /locus_tag="VBC81_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6912720.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKY"
BASE COUNT         2288 a         1058 c         1288 g         1773 t
ORIGIN      
        1 tttgaaaatc cacatatatt tttaatcttc cgtctgaaaa aataaataaa aatagtaaaa
       61 ataaacacga atttaaaata agcaaatttt ttaagaaaat ctgtgctaaa ctttaatagt
      121 tttgtgctta ataataatca gcacttacaa agaacaaagg gaaaagcgag gagagaactt
      181 ttaatgaaat tatcgaagaa gttattgttt tcggctgctg ttttaacaat ggtggcgggg
      241 tcaactgttg aaccagtagc tcagtttgcg actggaatga gtattgtaag agctgcagaa
      301 gtgtcacaag aacgcccagc gaaaacaaca gtaaatatct ataaattaca agctgatagt
      361 tataaatcgg aaattacttc taatggtggt atcgagaata aagacggcga agtaatatct
      421 aactatgcta aacttggtga caatgtaaaa ggtttgcaag gtgtacagtt taaacgttat
      481 aaagtcaaga cggatatttc tgttgatgaa ttgaaaaaat tgacaacagt tgaagcagca
      541 gatgcaaaag ttggaacgat tcttgaagaa ggtgtcagtc tacctcaaaa aactaatgct
      601 caaggtttgg tcgtcgatgc tctggattca aaaagtaatg tgagatactt gtatgtagaa
      661 gatttaaaga attcaccttc aaacattacc aaagcttatg ctgtaccgtt tgtgttggaa
      721 ttaccagttg ctaactctac aggtacaggt ttcctttctg aaattaatat ttaccctaaa
      781 aacgttgtaa ctgatgaacc aaaaacagat aaagatgtta aaaaattagg tcaggacgat
      841 gcaggttata cgattggtga agaattcaaa tggttcttga aatctacaat ccctgccaat
      901 ttaggtgact atgaaaaatt tgaaattact gataaatttg cagatggctt gacttataaa
      961 tctgttggaa aaatcaagat tggttcgaaa acactgaata gagatgagca ctacactatt
     1021 gatgaaccaa cagttgataa ccaaaataca ttaaaaatta cgtttaaacc agagaaattt
     1081 aaagaaattg ctgagctact taaaggaatg acccttgtta aaaatcaaga tgctcttgat
     1141 aaagctactg caaatacaga tgatgcggca tttttggaaa ttccagttgc atcaactatt
     1201 aatgaaaaag cagttttagg aaaagcaatt gaaaatactt ttgaacttca atatgaccat
     1261 actcctgata aagctgacaa tccaaaacca tctaatcctc caagaaaacc agaagttcat
     1321 actggtggga aacgatttgt aaagaaagac tcaacagaaa cacaaacact aggtggtgct
     1381 gagtttgatt tgttggcttc tgatgggaca gcagtaaaat ggacagatgc tcttattaaa
     1441 gcgaatacta ataaaaacta tattgctgga gaagctgtta ctgggcaacc aatcaaattg
     1501 aaatcacata cagacggtac gtttgagatt aaaggtttgg cttatgcagt tgatgcgaat
     1561 gcagagggta cagcagtaac ttacaaatta aaagaaacaa aagcaccaga aggttatgta
     1621 atccctgata aagaaatcga gtttacagta tcacaaacat cttataatac aaaaccaact
     1681 gacatcacgg ttgatagtgc tgatgcaaca cctgatacaa ttaaaaacaa caaacgtcct
     1741 tcaatcccta atactggtgg tattggtacg gctatctttg tcgctatcgg tgctgcggtg
     1801 atggcttttg ctgttaaggg gatgaagcgt cgtacaaaag ataactaaat aaaaggctac
     1861 ttcttaagta accatgttta agaaaagaga aatagcctta tttctctttt tgtcgttttt
     1921 aaaataaagg aacatcatga aacaaacatt aaaacttatg ttttcttttc tgttgatgtt
     1981 agggactatg tttggaatta gccaaactgt tttagcgcaa gaaactcatc agttgacgat
     2041 tgttcatctt gaagcaaggg atattgatcg tccaaatcca cagttggaga ttgcccctaa
     2101 agaagggact ccaattgaag gagtactcta tcagttgtac caattaaaat caactgaaga
     2161 tggcgatttg ttggcacatt ggaattccct aactatcaca gaattgaaaa aacaggcgca
     2221 gcaggttttt gaagccacta ctaatcaaca aggaaaggct acatttaacc aactaccaga
     2281 tggaatttat tatggtctgg cggttaaagc cggtgaaaaa aatcgtaatg tctcagcttt
     2341 cttggttgac ttgtctgagg ataaagtgat ttatcctaaa atcatctggt ccacaggtga
     2401 gttggacttg cttaaagttg gtgtggatgg tgataccaaa aaaccactag caggcgttgt
     2461 ctttgaactt tatgaaaaga atggtaggac tcctattcgt gtgaaaaatg gggtgcattc
     2521 tcaagatatt gacgctgcaa aacatttaga aacagattca tcagggcata tcagaatttc
     2581 cgggctcatc catggggact atgtcttaaa agaaatcgag acacagtcag gatatcagat
     2641 cggacaggca gagactgctg tgactattga aaaatcaaaa acagtaacag taacgattga
     2701 aaataaaaaa gttccgacac ctaaagtgcc atctcgagga ggtcttattc ccaaaacagg
     2761 tgagcaacag gcaatggcac ttgtaattat tggtggtatt ttaattgctt tagccttacg
     2821 attactatca aaacatcgga aacatcaaaa taaggattag catgggacaa aaatcaaaaa
     2881 tatctctagc tacgaatatt cgtatatgga tttttcgttt aattttctta gcgggtttcc
     2941 ttgttttggc atttcccatc gttagtcagg tcatgtactt tcaagcctct cacgccaata
     3001 ttaatgcttt taaagaagct gttaccaaga ttgaccgggt ggagattaat cggcgtttag
     3061 aacttgctta tgcttataac gccagtatag caggtgccaa aactaatggc gaatatccag
     3121 cgcttaaaga cccctactct gctgaacaaa agcaggcagg ggtcgttgag tacgcccgca
     3181 tgcttgaagt caaagaacaa ataggtcatg tgattattcc aagaattaat caggatatcc
     3241 ctatttacgc tggctctgct gaagaaaatc ttcagagggg cgttggacat ttagagggga
     3301 ccagtcttcc agtcggtggt gagtcaactc atgccgttct aactgcccat cgagggctac
     3361 caacggccaa gctatttacc aatttagaca aggtaacagt aggtgaccgt ttttacattg
     3421 aacacatcgg cggaaagatt gcttatcagg tagaccaaat caaagttatc gcccctgatc
     3481 agttagagga tttgtacgtg attcaaggag aagatcacgt caccctatta acttgcacac
     3541 cttatatgat aaatagtcat cgcctcctcg ttcgaggcaa gcgaattcct tatgtggaaa
     3601 aaacagtgca gaaagattca aagaccttca ggcaacaaca atacctaacc tatgctatgt
     3661 gggtagtcgt tggacttatc ttgctgtcgc ttctcatttg gtttaaaaag acgaaacaga
     3721 aaaagcggag aaagaatgaa aaagcggcta gtcaaaatag tcacaataat tcgaaataat
     3781 aaaatcagaa ccctcatttt tgtgatggga agtctgattc tcttatttcc gattgtgagc
     3841 caggtaagtt actaccttgc ttcgcatcaa aatattaatc aatttaagcg ggaagtcgct
     3901 aagattgata ctaatacggt tgaacgacgc atcgctttag ctaatgctta caatgagacg
     3961 ttatcaagga atcccttgct tatagaccct tttaccagta agcaaaaaga aggtttgaga
     4021 gagtatgctc gtatgcttga agttcatgag caaataggtc atgtggcaat cccaagtatt
     4081 ggggttgata ttccaattta tgctggaaca tccgaaactg tgcttcagaa aggtagtggg
     4141 catttggagg gaaccagtct tccagtggga ggtttgtcaa cccattcagt actaactgcc
     4201 caccgtggct tgccaacagc taggctattt accgacttaa ataaagttaa aaaaggccag
     4261 attttctatg tgacgaacat caaggaaaca cttgcctaca aagtcgtgtc tatcaaagtt
     4321 gtggatccaa cagctttaag tgaggttaag attgtcaatg gtaaggatta tataaccttg
     4381 ctgacttgca caccttacat gatcaatagt catcgtctct tggtaaaagg agagcgtatt
     4441 ccttatgatt ctaccgaggc ggaaaagcac aaagaacaaa ccgtacaaga ttatcgtttg
     4501 tcactagtgt tgaagatact actagtatta ttaattggac tcttcatcgt gataatgatg
     4561 agaagatgga tgcaacatcg tcaataacga tgttgtgaat ggcttactta cttatcaaat
     4621 aggtgactaa tgatgattgt gaataatggt tatctagaag ggagaaaaat gaaaaagaga
     4681 caaaaaatat ggagagggtt atcagttact ttactaatcc tgtcccaaat tccatttggt
     4741 atattggtac aaggtgaaac ccaagatacc aatcaagcac ttggaaaagt aattgttaaa
     4801 aaaacgggag acaatgctac accattaggc aaagcgactt ttgtgttaaa aaatgacaat
     4861 gataagtcag aaacaagtca cgaaacggta gagggttctg gagaagcaac ctttgaaaac
     4921 ataaaacctg gagactacac attaagagaa gaaacagcac caattggtta taaaaaaact
     4981 gataaaacct ggaaagttaa agttgcagat aacggagcaa caataatcga gggtatggat
     5041 gcagataaag cagagaaacg aaaagaagtt ttgaatgccc aatatccaaa atcagctatt
     5101 tatgaggata caaaagaaaa ttacccatta gttaatgtag agggttccaa agttggtgaa
     5161 caatacaaag cattgaatcc aataaatgga aaagatggtc gaagagagat tgctgaaggt
     5221 tggttatcaa aaaaaattac aggggtcaat gatctcgata agaataaata taaaattgaa
     5281 ttaactgttg agggtaaaac cactgttgaa acgaaagaac ttaatcaacc actagatgtc
     5341 gttgtgctat tagataattc aaatagtatg aataatgaaa gagccaataa ttctcaaaga
     5401 gcattaaaag ctggggaagc agttgaaaag ctgattgata aaattacatc aaataaagac
     5461 aatagagtag ctcttgtgac atatgcctca accatttttg atggtactga agcgaccgta
     5521 tcaaagggag ttgccgatca aaatggtaaa gcgctgaatg atagtgtatc atgggattat
     5581 cataaaacta cttttacagc aactacacat aattacagtt atttaaattt aacaaatgat
     5641 gctaacgaag ttaatattct aaagtcaaga attccaaagg aagcggagca tataaatggg
     5701 gatcgcacgc tctatcaatt tggtgcgaca tttactcaaa aagctctaat gaaagcaaat
     5761 gaaattttag agacacaaag ttctaatgct agaaaaaaac ttatttttca cgtaactgat
     5821 ggtgtcccta cgatgtctta tgccataaat tttaatcctt atatatcaac atcttaccaa
     5881 aaccagttta attctttttt aaataaaata ccagatagaa gtggtattct ccaagaggat
     5941 tttataatca atggtgatga ttatcaaata gtaaaaggag atggagagag ttttaaactg
     6001 ttttcggata gaaaagttcc tgttactgga ggaacgacac aagcagctta tcgagtaccg
     6061 caaaatcaac tctctgtaat gagtaatgag ggatatgcaa ttaatagtgg atatatttat
     6121 ctctattgga gagattacaa ctgggtctat ccatttgatc ctaagacaaa gaaagtttct
     6181 gcaacgaaac aaatcaaaac tcatggtgag ccaacaacat tatactttaa tggaaatata
     6241 agacctaaag gttatgacat ttttactgtt gggattggtg taaacggaga tcctggtgca
     6301 actcctcttg aagctgagaa atttatgcaa tcaatatcaa gtaaaacaga aaattatact
     6361 aatgttgatg atacaaataa aatttatgat gagctaaata aatactt
//
LOCUS       DATRNX010000109         6081 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRNX010000109 DATRNX010000000
VERSION     DATRNX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..579)
                     /locus_tag="VBC81_001701"
     CDS             complement(<1..579)
                     /locus_tag="VBC81_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6912721.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGE"
     gene            788..1261
                     /locus_tag="VBC81_001702"
     CDS             788..1261
                     /locus_tag="VBC81_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6912722.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            1263..1568
                     /locus_tag="VBC81_001703"
     CDS             1263..1568
                     /locus_tag="VBC81_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6912723.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGSILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            1608..2095
                     /locus_tag="VBC81_001704"
                     /pseudo
     CDS             1608..2095
                     /locus_tag="VBC81_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
     gene            2336..4786
                     /locus_tag="VBC81_001705"
     CDS             2336..4786
                     /locus_tag="VBC81_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6912724.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANEPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
     gene            5035..5459
                     /gene="lacA"
                     /locus_tag="VBC81_001706"
                     /pseudo
     CDS             5035..5459
                     /gene="lacA"
                     /locus_tag="VBC81_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            5480..5995
                     /gene="lacB"
                     /locus_tag="VBC81_001707"
     CDS             5480..5995
                     /gene="lacB"
                     /locus_tag="VBC81_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6912725.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
BASE COUNT         2136 a          881 c         1153 g         1911 t
ORIGIN      
        1 ttcaccttct ccctcgtcat aagtagcgat agacttttca aaaacaccat tactactaac
       61 aaaagctttt gaaaaattaa gtgatttaat agagttaata gtaacagaac cgacaaaagc
      121 accagtaatt tctctgtact ctcctcctac aaggataagg tcaagtgttg aactttgatt
      181 cagaatattg aaaacaggaa ggctattggt tactacccta attttaaaat caagtaattg
      241 cgtagctaat ttctctaagg ttgttccagg cccaataaag attgtttcac catcattgat
      301 gaattgtttt gcaaattggg caatttctaa tttttcattt gtttgaatat cgtatttttc
      361 agtgtttgat ttttcataat tttgtggaat tggtgctgcg tttacttttt gtgcaccacc
      421 atggatgcgt attaacagtc ctgctttatc taattcatcc aagtcacgcc taacagtcat
      481 gtctgataca tcaagttctt ccatgatttc attaacagtg acaataccat ttatattgac
      541 tttttctatt attttttgta atctttctcg ctttaacata ataatctctc ctacctcttc
      601 attgtatata tcataccata aatagagcta aaataataat aattttgaaa aaacaaacaa
      661 taataaacaa aaataaataa aaaacgtctt gtgatgtttt attttgtttt gtataatatt
      721 gatgtagata agttgataag gcttgtttgc cttacaacaa aatgtaaccg cttaactaaa
      781 aagaaaggtg tttgctgtga ctcaagatat tttatttatt gatgcccatt ctcaggaaga
      841 attatttgac ttagtatcaa aagcattgat aaaacaacac tatgttagtc ctgactaccg
      901 acaggcagta aaagaaaggg agagagagtt tccaacgggg ttaaaaattg atttaaaaga
      961 tgggactcct attcaatatg ttgctattcc acacactgaa acgcagtatt gtctagtaga
     1021 tagaatcttt tatgtcaaaa atagtcagcc aattactttc aaacatatga ttaatccaga
     1081 agaggagtgt agggttcagg attttttctt catcattaat agtaggaata gtaaccaaag
     1141 tgatatttta tctaatttga ttaccttctt tattacaaaa ggaaacctag atcggctaca
     1201 tgagcttggc gacaataagg aaaaaattaa tcattattta atagaaaaag gagtgtttta
     1261 acatgatcaa aatattagca gcttgtggtg caggcataaa ttcaagtcat caaattaaag
     1321 atgctatcga gacacaatta ggagatagag gttacaatgt acattgtgat gctgttatgg
     1381 ttaaggatat cactgaggaa atggtcaaca aatatgatat ctttactcct attgctaaaa
     1441 ctgatttagg ttttaatgta ccaattccag ttgtagaagc aggatctatt ctttatcgca
     1501 taccagttat gagcgaaccg gtctttactg ctttagaaca agttatcaaa gaacacaacc
     1561 tatcataaca tatttaaaat tggaattagt cttaaaggag aaggaacatg caggtatttt
     1621 taaatattgt caataaattc tttgatccag ttattcatat gggttcggga gttgtgatgc
     1681 taattgtcat gacaggttta gccatgatat ttggagtgaa gttttctaaa gcacttgaag
     1741 gtggtattaa gttagctatt gctcttacgg gtattggtgc tattattggt attttaactg
     1801 gtgctttttc cgaatcactt caagcttttg ttaaaaatac aggaatcaat ctaagcatta
     1861 ttgacgttgg ttgggctcca ttagcaacta ttacatgggg atcaccatat acgctttact
     1921 tcttattaat catgcttatt gtcaatattg ttatgattgt tatgaaaaaa aaacggatac
     1981 cttagatgtt gatatttttg atatttggca tttatctatc acaggtcttt taattatgtg
     2041 gtatgctaaa caaatgaaac gccgtaatgc agaatatgca gctaatcaaa aataaataga
     2101 ataaacaaaa acctaggaaa tatcacattc tcctaggttt ttgatatatt aaatatttta
     2161 aaaataatat atataattaa ttttcgctca ttttaataat aaaaaactag taaaattata
     2221 aaaatatatt gaaaatagtg ataagttggt atacaatcct ttttggaaac attactgaac
     2281 tcccattttt ctagagttga tgtatgcaac caatatttta agacttggag tttctatgac
     2341 attaaccttt gggaaacctt ctataacgat acgccgtcta ggagtgataa ctagtacata
     2401 tatgatgcta ggttttatct tattatgtgg acttttgcat cctagtacaa tttatagtga
     2461 ggaaataaaa tctgatataa actcttttat atcaatagat gatgaaaaaa ctactgctcc
     2521 catatttgac tctactttag ataagcttac taattcaaca gaggagaggc agttagaatt
     2581 agattctgat acatcggacg gtttaatagt agatgatcaa aaagataaag atatggtagt
     2641 agtcttaggt gattctgaca gtcaagaaaa attgtcacct cctctaaaat cggaaatagc
     2701 gacagaagaa aatcgtaaac aggcaaattt agcagaacta aatgcaactc aacctaataa
     2761 tagaactacc tatattatac ccgaaagcag tcattccatt gcagaacaac agagattcct
     2821 gatagaatca aagggttctt cggttgcatt acttaatagc gatgaattta gaaagacagc
     2881 gggagaggat agaggttttg aaagggataa gttgaggtct ttggatatca ttcctaaggg
     2941 agatttatcg acaagtaatg tcataggtaa tacggacatt gctagtcaga tatcgttggg
     3001 ctttaaaaag aatgcgatgc aggaacacca tcttactaaa acattctctc aaaaggatgg
     3061 aaagttatcg tctgttatag aggggatgct tgctattggc aaagagaaag tagagaaaga
     3121 aataaaatat agtggtaatt tatggcaaaa attaaaagct aaggcacact gccttgtttg
     3181 ctgtgttgat aatttgaatt ttgaagatat aaaatcttat tttcaatatt attgtcatct
     3241 aaaccatcag ctcaaattac ctaaaggtgc tatactttct gctaaaacag aagtatatag
     3301 gggaggagat tttgggagaa aaaataaaga taatgtgttt ggttaccgta tcccctcatt
     3361 attgaaaacc caaaaaggaa ctttacttgc gggagctgat gaaagaattg agcaagcttg
     3421 tgattgggga aacataggaa tggttattcg ccgtagtgag gatgatggtg tcacttgggg
     3481 aaaaagagaa actattgtca atctccgtaa taaccctaga gttccgctag ttactagtgg
     3541 tgactatagt ggctcaccta ttaatatgga tatggcatta gttcaagata ctagctccaa
     3601 gacgaaacgt attttttcaa tatatgatat gtttccagaa ggaagaggcg ttattagtat
     3661 tgctaacaca cctgaaaaag aatataccca aatcggagga cagtcttatc ttaatttata
     3721 taataatgga aagaaatcga aggtttttac tatccgtgac aaaggtattg tatataattt
     3781 taaagggaaa aagactgatt atcatgttat aacagaaact actaaaagtg accattcaaa
     3841 tctaggggat atttataagg gaaaacagct acttggaaat atatatttta caaaacataa
     3901 aacgtcacca tttcgtttag caaaatcaag ctatgtgtgg atgtcatata gcgatgatga
     3961 tggtaggaca tggtcatcac ctagagatat aacagcaagt cttcgtcaga aaggcatgaa
     4021 atttttggga ataggacctg gaaaaggtat agttttaaaa tgggggccac acgctggtcg
     4081 tattattatt cctgcctatt ctacgaattg gaaatctcat ctaagaggtt cacaatcttc
     4141 acgcctaatt tattcagacg accatggaaa aacgtggcat actggaaaag cagttaatga
     4201 taaccgtata ctttctaatg gtgaaaaaat tcactcctta acaatggata ataaaaaaga
     4261 acaaaataca gaatccgtac ccgttcaatt gaaaaatggg gacattaagt tatttatgag
     4321 gaatctaact ggtaacctag aagtagccac aagtaaagac ggcggggaga cttggcaaaa
     4381 ccatgttaaa cgatataagg aaattcatga tgcttacgtc caactatcag ctattcgctt
     4441 tgagcatgac aaaaaagagt atattttatt agtgaatgct aatgagccag ggaagaagtg
     4501 ccaagatgga tatgcacgtc tagcgcaagt taatcgaaat ggtagtttta agtggttata
     4561 tcaccatcac attcaagatg gttcgtttgc ttacaactct gttcaacaac ttaataatga
     4621 tcaatttggt gtcctttatg aacatagaga aaaacatcaa aatagtttta ctttaaatta
     4681 caaagttttt aattggagtt ttcttagtca aaatacagag aagcaaggca ctttatggga
     4741 gaaaatggca gcaaattggc atgttttgtt taaattttat ttatgattat ggcttgcgga
     4801 ataaccgttc ctacaaataa aaataatgag aagtatcaaa cttcgtgata gctagctagt
     4861 agtgaattct agttgccata gttatcacgg agtttttttg tttatttcaa acaaaaacaa
     4921 aaacaaaaca ttgacaaaaa caaaaataag atatatgatg atattacaaa caaaaaacaa
     4981 acaatatctt ccaactacat acaattgtat taaaagtgaa ataaggagaa agaaatgaca
     5041 attattattg gggcggatgc gcatggtgtt gaacttaagg aagtgattag acaacacctt
     5101 acacgtttag gtaaggaaat tattgattta acggatacta gcaaagattt cgttgataat
     5161 accttagcaa tcgttgctaa agtgaatcaa aaagaagata acttaggaat aatggttgat
     5221 gcttacggtg ctggtccttt tatggtagcc actaaggtta aggggatgat tgcagcagaa
     5281 gtttcagatg aacgctcagc ttatatgact agagctcata ataacgctag gatgattact
     5341 ttaggttcag agattgttgg tccggggttg ctaaacatat agtagagggt tttgttgatg
     5401 ggacttacga tgcaggtcgt catcaaattc gggtcgatat gttgaataaa atgtgctaag
     5461 cgtaagagag gtaaagcaga tgaaaattgc ggtcggttgt gatcatattg taacttatga
     5521 caaaattgca gttgtggatt atttaaaaac aaaagggtat gaggttattg attgtggaac
     5581 ttatgataac atccgtactc actatcctat ttatggtaag aaagtaggag aagctgtcgc
     5641 tagtggaaaa gccgacttag gtgtatgtat ttgtggaact ggagttggta ttaataatgc
     5701 tgttaataaa gttccaggaa ttcgctcagc tctggtacgt gatttgacat cagctatcta
     5761 tgctaaagaa gaattaaatg ctaacgttat cggatttggt gggaaaatta ctggtgggct
     5821 gttaatgaca gatatcatcg aggcgtttat aagagctaaa tataaaccaa caaaagaaaa
     5881 caaagtatta attgaaaaga tagccgaggt tgagactcat aatgcccatc aagaagagaa
     5941 tgactttttc acagagttct tagataaatg gaatcgtggt gaatatcacg actaaggaga
     6001 tatctatgat tttaacagta actttgaacc cttctattga tatttcgtac tgcttagaaa
     6061 actttaacat ggatactgta a
//
LOCUS       DATRNX010000110         6077 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRNX010000110 DATRNX010000000
VERSION     DATRNX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBC81_001708"
     CDS             complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBC81_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6912726.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            1959..2726
                     /locus_tag="VBC81_001709"
     CDS             1959..2726
                     /locus_tag="VBC81_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6912727.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(2785..4125)
                     /locus_tag="VBC81_001710"
     CDS             complement(2785..4125)
                     /locus_tag="VBC81_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6912728.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4225..4641)
                     /locus_tag="VBC81_001711"
     CDS             complement(4225..4641)
                     /locus_tag="VBC81_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6912729.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(4645..5142)
                     /locus_tag="VBC81_001712"
     CDS             complement(4645..5142)
                     /locus_tag="VBC81_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6912730.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5364..5960
                     /locus_tag="VBC81_001713"
     CDS             5364..5960
                     /locus_tag="VBC81_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6912731.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         2098 a         1150 c          903 g         1926 t
ORIGIN      
        1 actgttctac taagtttttt atttaggagg atcattaaga gaatctcgga gactttctgc
       61 aactgtccta ggaataccac acataataat gtcctcaata tcagcttttt ggatattagg
      121 taagctttta aaatgtttca ttagcaattg tttacgtttt ggtcccaacc cagtgatacc
      181 atctaatttt gaagaaaagg aatttttgga gcgtaattga cgatgaaaag taatagcaaa
      241 acgatgtacc tcatcttgaa tacggtgaag taagaaaaat tcttctgaat tacgcggtaa
      301 ttctactact tctaaaggat ctccaaaaag taattcatgt gtttggtgtt tatcattctt
      361 ttgcaaacct gcaataggta tggctaaacc taattggttc tcaataacat cacgcgctat
      421 atttacttgc ccctgaccac catcaattac aatcaagtcc ggaggtgtca agccatcttt
      481 taaaacccta ctataacgcc gatgaatgac ttctctcata ctagcataat catctggtcc
      541 gataacagtc ttaattttaa actttcgata gtctttttta cttggatttc cattcacaaa
      601 taccaccata gcagctactg gacttgtccc ttgaatattg gaattatcaa atgcttctat
      661 gcgtactggt ttagggatat taagaagatt acctaaattt tcaattgccc catgagtttt
      721 acgaatatct ttttccagta agtcaaactt ctgttgtaaa ctgacacgag cattttttat
      781 tgccagattt actaactgtt tcttttcacc acgttgtggt ttaacaatct tacagcctac
      841 aattgtttca accgacttag catcaatatc ctgtggaata aagacttctt tgggtagaaa
      901 atgttttgta tcttgataaa attgccctat gtaagttaga aaatcttcct ctggttcatt
      961 ataataagga aacatattca cgtcacgctg aatcaatttt ccatttcgaa caaaaaagac
     1021 ctgtacacac atccaaccct tatcaacgaa ataaccaaaa acatctctat ccttcatatc
     1081 ttgatgaata acacgctgct tagtacgcaa caaactaatt gcttcgatca aatctcgata
     1141 ttcagccgca cgctcaaatt ccatagtcat agcagcgctc ttcatttttt cagttaatcc
     1201 gttaacaatt ttattatctt taccattaag aaactgtttt acatcttctc tcagactatc
     1261 ccaataagct ttattagttt gacaaactgt atgtgcattg cattgcccta aatgataata
     1321 aaagcatact ttatttgctg gattagtaca tttcttaaaa ggaaagagcc tatctagtaa
     1381 ccgcttaatc tcagttgctg ccccagaatc cggataaggt ccaaaataag tgccatctga
     1441 tttttttact tgacgtgtaa tcataagacg aggataacgt tcatttgtta ttttgatata
     1501 tgggtaagac ttatcatctt tcaatcgaat attatacttc ggcatatttt cttggattaa
     1561 attaatttcc aataatagag cttcggtatt agatgttgtc acaatgtatt caaagtcttc
     1621 aatctcagag accaaaagtt ctgtcttagt attatgactc ccatgaaaat aacttttcac
     1681 acgattctta agatttttag ccttacctac ataaataatt gtcccattct tatctttatg
     1741 aaggtaacaa ccaggactgt ctggtaacaa ttctagctta tgtttaatca gttcattcat
     1801 agaactatta taacaaaatg tactgtaatc tttacgaaat aaatactatt ccccaacatc
     1861 tattgtatct tccaatactt gaataaaacc ccaatactcc tagtctaagc aattatttta
     1921 tgttacactt taaacaagtg aattattgag gagtttttat gatagacgtt gagaatattc
     1981 ttgaaaaaat gaaacctaac caaaaaatca attacgattg ggtcatgcaa caaatggtta
     2041 agcagtggca agctagtgat atccgtccga aaattctgat gcatgtttgc tgtgctcctt
     2101 gtagtacata tacccttgaa tacttatcac aatgggcaga tgttaccatc tattttgcta
     2161 attcaaatat ccatcctaaa gatgaatatt atcgccgcga atatgttact caaaaatttg
     2221 ttcatgattt caacaaaaat actggatatt ctgtacaatt cttatcagca ccatatgaac
     2281 caaatgaatt tttcaaaatt gttcatgggt tagaagaaga acctgaaggt ggggatcgtt
     2341 gtaaagtttg ctatgatttc cgtcttgata agacagcgga aaaagccgtt gaactaggtt
     2401 ttgattactt tggcagtgcc ctaactatta gtcctcacaa aaactctcaa acaatcaata
     2461 ctattggtat tgatgtccaa aaaatttatg atactcagta tcttcctagc gaccttaaaa
     2521 aaaataaagg ttaccaacgt tccgttgaga tgtgtaaaga ttatgatatc taccgacaat
     2581 gttactgtgg gtgtatcttc ggtgctaaag atcaagggat aaatcttctc caaatcaaaa
     2641 aagatgcgaa agctttcgtg agtgataaag acggcaaaga agaatttcct aatatacgct
     2701 tcacatttaa tggcaagtca atgtaaatga taaaaaaaga cttagtaaag aactaagcct
     2761 ttttggtata aatcttgtca aactttaata atacaacttc ttaatctttt gcagacgttg
     2821 gtttagaaat agaccacaaa caaaaatacc tgtgattaaa ccaatccaat aggcaaacgg
     2881 acctaactgg ctattttttt ctaagataac cgctaatggc aaagcacata accaataaga
     2941 gcccgcaccg atcataaatg gttttgttgt atccttatag cctcgtaaaa tcccctgtac
     3001 aggagctgca taagcatctg caaactggaa aaagagacta taagttagga attgagctgt
     3061 aatagcgaca aagtgagggt cactattata cattgctgct acattctcac gaaatagaaa
     3121 taaaaatagt aaggttcctg atgtaatccc taccgctgtt aagcgtccta tcctactata
     3181 agtgtttgcg tcctgaaagc gctctgcccc tacttcaaac gatattgtaa tagctagagc
     3241 agtggaaata cttaaaggaa atgcatacat taatgatgaa aaattcatag cagcctgatg
     3301 tgctgcaata atgattgaag aaaattttgc catgaataag cctactactg caaaaattgc
     3361 aacttctgca aaaatttgta aaccaatcgg taatcccaat cgaatatctt caataatcaa
     3421 aggagctttt attctttcca gagtccatat atgatatgtt ttaatttgag gatgaagtga
     3481 catcacaata ataataacaa taaaaatagc ccaataagtt aaagaagttc caagacctgc
     3541 ccccgcacct cctagtctag gcataccaaa tttaccgtag ataagcatat aattaaaaaa
     3601 tgaattaaag ggtagaatta aaagcatcag atacatagat aaccttgtta accccaatgc
     3661 atcaaagaat gaacggcaaa tgctaaacaa caccagcggc atgattccaa tcaacatata
     3721 atttaaataa ccacgaccaa ctgctagaac ttcatcttct aaacccaaac tccccaagac
     3781 aggttgagca ataaattgca tgattaaaaa taatattaag gacagtatca aacctaaata
     3841 tagaaattga tgaaattctg tgcgaatttg ttctttattt cctctaccca aatgttgacc
     3901 aactactggt actaatgctg aaatcatacc tactaataaa gcgaaaaacg gagtccataa
     3961 attactagca gttgacacac ctgccaaatg tagctgacta tactgtccag tcataaccga
     4021 atcaataaaa gtagctgaaa aattagcaaa ttgataaatc aatactggga taaataattt
     4081 caaaaataaa acaaattttt ccttatttgt ctgagtttga tacatctttt ttctcctttt
     4141 caccttaatc aatttatttt agcttagcta ggatactctg tcaatgctat ttaaaaaggt
     4201 tgagaaataa tcctcaaccc cattttagct aagctcagta ttgatttgcc atgtcatacc
     4261 atatttgtca gtcacatttc cataagctgg actccacgga actgcttgta gaggcatttt
     4321 aatgttcatt gcatctcgag aaagcttatt gaataactct tgcgcttctt ctacagattc
     4381 cacaattaaa gctgctgtca tattttgtcc aatttgaaaa tcaaaaatag gattattgtc
     4441 agaaagttgc attcgaatac catcaatatt aaattgagcg ttcataatta aatgttcaag
     4501 ttctggcgga caatctggcc aaccctgtgc aaaagttgtg acgtgttctg cttttgtatt
     4561 aaaagcctct tcataaaatg taatagcttc ggccccacga ccattaatat tcatataaat
     4621 ttcaattgct ttcattacca tacctcactt catattatcc aaccaacgct gcatcagagt
     4681 ttcattttcc ccttttaggg taaaataaga accacgtatt atacctgcga gttttgactt
     4741 ttgacgagca aacttaaact tgtccgctaa ttcaatagga atatccattc catctgataa
     4801 ttttaaacct acagctctct ttttaggatt atctaattca tataaaatat taatggctaa
     4861 accatcctca tagaaaacat gtgtccataa aatacgattc tctaaaaaaa gctgtgaaat
     4921 ctctaaaggc tttgcagcaa aaacaaggtt acgttcattt tttagaaggt gattaatatc
     4981 tgccatcaat tctggatagt ctgatactgg aaatgtttcg taggctactt tgaatttatt
     5041 ccagaaatag cgagcttcat ttgcacgcaa accagccaag gcttctgcca ctggagaagt
     5101 ctccaaacct ttagttgaaa cggttttaaa gtcgataatc atgttctttc ctttttgatt
     5161 aaaatcttcg ataatttagt atactctttt ttgaagaaaa agtacattct aatcacttat
     5221 aaaaagtagt acatttagaa cactaccacc tcagtaattt cttgaccttt ctctacttaa
     5281 tacttataat taatctagac ttaatgtcaa agcatcaatc aaggtgtaaa cctgattcac
     5341 ctaaaaagct gatggagcta aacatgacca aaaagatatt cttatttatc tgcgctactc
     5401 ttttagtagg aatagcctta tatacaccaa caacttctat ttttaatcat aacaacgttt
     5461 atgctgccac caagaaagca aaaattaaat ttaataaaac tcaaaaaaaa attgtcaaaa
     5521 aagcaagaga atatgctaag tctggccata tgtctaaaga tagtatcatt gaaaaattaa
     5581 aaaaggattc taagaaatat cgtcaagaag acatcaactt tgtgattaat aacctcaagg
     5641 ttgattacaa aaaaaatgcc ttgatttctg ccaaaattta ttcaaagacc atgaatttat
     5701 ctaaacaatc tatctttgaa cagctctatt cggagtctcc agataaggca acccactctg
     5761 ataaatttac aaaagaagaa tcccaatatg ctatagacca tctcaaagta gatttcaaag
     5821 aaaatgctct tgagactgct aaaagttatc aaagtagttc ttctttatcc aaagaagaaa
     5881 tctataaaca attgacatct acgctgggcg ataaattcac aaatgatgaa gcccaatatg
     5941 ctgtagatca tttgaaatga tgacatacca atagtctatc cttatgtaat agaattgata
     6001 gcttatgcta atattccagc tgtaaaatag caagaaccct cctaagttca ttaagactta
     6061 ggagggtttt tatcaat
//
LOCUS       DATRNX010000111         6046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRNX010000111 DATRNX010000000
VERSION     DATRNX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            268..1089
                     /locus_tag="VBC81_001714"
     CDS             268..1089
                     /locus_tag="VBC81_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6912732.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1190..1804
                     /locus_tag="VBC81_001715"
     CDS             1190..1804
                     /locus_tag="VBC81_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6912733.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            1964..3403
                     /locus_tag="VBC81_001716"
     CDS             1964..3403
                     /locus_tag="VBC81_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6912734.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSHKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3494..4627
                     /locus_tag="VBC81_001717"
     CDS             3494..4627
                     /locus_tag="VBC81_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6912735.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            4723..5403
                     /gene="radC"
                     /locus_tag="VBC81_001718"
     CDS             4723..5403
                     /gene="radC"
                     /locus_tag="VBC81_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6912736.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(5416..>6046)
                     /locus_tag="VBC81_001719"
     CDS             complement(5416..>6046)
                     /locus_tag="VBC81_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6912737.1"
                     /translation="DKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGIDSA
                     TVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHYRFH
                     DRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTEAQE
                     VADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
BASE COUNT         1984 a          898 c         1146 g         2018 t
ORIGIN      
        1 atattttatt atgactattg taatagttac gaatctatta ttcaactcaa caataagaaa
       61 actcttcagt aaatcattat gttaaacaag ctctgatagt atggagataa gtataggtaa
      121 aagtcgattt aatctctgaa acaaaattta acttaattaa tagaaccgtt gacgtatcag
      181 gtatcaaatc tttttggttt atatgtgata taatgaaaga acatattgag atatttggca
      241 ccctcttgaa aggcaaaggt tacttgtatg attaaactaa tagcaacaga tatggatgga
      301 acttttctac gctcagataa aacttatgat aaagcacggt tttcttcttt attaacgttg
      361 atggagaagt atgatattaa atttgtagct gctagtggta atttatacga tcaattgttg
      421 ttaaactttt tggaataccc taatcgtatc gcctacgtcg ctgaaaatgg tggtcgtgtc
      481 attgaccaag atggcacatt actaaaagaa acttatttat ctaatgatac agttgcagct
      541 gttttatcct atttatatca gaactatcct gagacactca tcagcttatc tggtgaaaaa
      601 cgttcatacc ttgagcgaag aactcctatt aataggcgga ctgaattaga atactatatg
      661 cctaatttta tctataaaga ccatttgctg cccttagatg atgaccgata ttttcaaatg
      721 acactttggg taaatgaaaa tcttgtttct gagatgttgt tagatattag tgaacacttt
      781 aaaaatcatc atatcagatt gacctctagt ggttttggtt gtatcgatgt cttaccagct
      841 gatgtcaata aagcggatgg tatcgctatt ctccttgaaa agtggggatt gaaacaggat
      901 caagtgatgg tttttggtga tgggggcaat gatgtggaaa tgttgagagc agcaaatatt
      961 tcttacgcta tgtcaaatgc gccagaagaa attaaagcta ttgctaaata tcaaacagtt
     1021 agtaacgatc aagatggtgt gttagaaacg atagagaact ttcttcacaa gatgagtgat
     1081 aatgaataga accacgtagc ttttcacgaa gcatactatt caattctcag gtgaaatttt
     1141 gttataatac aagaaagcct agagaaatat ttaagaatga aggagaacga tgttagaagt
     1201 tattgacaaa gctttaagag attatcaaat gaaacgagaa cagttttttg agattaacaa
     1261 tcagacagta caagaaggtg ccattgtttt cacaggagat tctatcgttg agttttttcc
     1321 tttaaaaaaa catttaggac gtgactatcc tttagttaat agaggagtag caggatcaga
     1381 ttcttattgg cttttagaaa acttgagaac ccaagtatgg gaactattac catccaaagt
     1441 atttatttta attggaacaa atgatattgg tttagggcat tcacagtcag aaatcatagc
     1501 taatatcacg gatataattg cagaaattcg tgcggaatct tatatgacag agattaatat
     1561 actgagtgtt ttacctgtta gtgaagaaga tgactatata gagcgtgtca aggtaagaaa
     1621 taatcaagca ataaaagctt taaacaaaac cttatcagta atttctggca ttaattatat
     1681 tgaattgtat gatttattag ttgacgaaaa aggtcagctt gctagcagtt ttacaaaaga
     1741 tggtttgcac ctaacagatc aagcctatgc taaaatttct gaaacgataa aactccacct
     1801 ttaaaagtgt ttagatacta tttttaagta tttaaataca cagaattagt gaaaagacct
     1861 tctaagtaga aagttcatgt cacggatagt accagtattt ggcgaatcaa tgtcagtaac
     1921 tggtcttttt taatattatg ataagaaaaa acaagagggg taaatgatgg taaaacaagt
     1981 atttccaaaa ggttttttat ggggcggggc aactgctgcc aatcaatgtg aaggagctta
     2041 caatgttgat ggacgtggcc tagcaaatgt agatgttgtt cctactggag aagatcgatt
     2101 tgcaattatt tcaggacaaa agaaaatgtt tgattttgag gaaggatact tttatccagc
     2161 aaaagaatca atagattttt atcaccatta taaagaagac cttgcattac ttgcagaaat
     2221 gggtttcaaa acctatcgta tgtcaatagc atggacgcgt atttttccaa agggtgatga
     2281 gctatatcca aatgaagctg gtcttcggtt ttatgaaaat atttttaaag agtgtcgtaa
     2341 gtatggtatt gaacctttgg taaccatcac acattttgac tgtcctatct accttattaa
     2401 acattacggt gggtggcgga gccataaaat gattggtttt tatgagcgcc ttgtacgagc
     2461 tttatttact cgttttaagg ggttagttaa atattggttg acctttaacg aaatcaatat
     2521 gattttacac gctcctttta tgggcgctgg cttatatttt gaagatggtg aaaatcaaga
     2581 gcaaattaaa tatcaagcag ctcaccatga gttagtggct tcggctattg cagtaaaaat
     2641 tgctcatgaa gttgatccaa atagtcaaat cggatgtatg ttagctgcag gtcaatacta
     2701 tccaaatacg tgtcatccac aagattattg ggcttcgatg caaaaaaata gagaaaatta
     2761 tttttttatt gatgtgcaag cgcgtggcaa ataccctaat tatgctaaaa aatattttga
     2821 gcatttaggt atttcaattc aaatgacagc agaagatctt gctttgttaa gagactatac
     2881 ggttgatttt atttcatttt cttactattc aagccgagta gcttcaggta atccaactgt
     2941 cagtgaacag gttcaagaaa atattttcgc atctcttaag aatccttact tgaaatcttc
     3001 tgaatgggga tggcaaattg atccgcttgg attacgcatt accttaaatg ctatctggga
     3061 tcgttatcaa aaaccgatgt ttattgttga aaatggactt ggagcagtag atattcctga
     3121 tgagaatggt tacgtagaag atgattatcg tattgattac ctccgtcagc acattgctgc
     3181 aatgagagat gctatatatg tagatggtgt taatctaatt ggctatacta catggggatg
     3241 tattgattta gtatctgcag gaactggtga aatggaaaaa cgctatggtt tcatttatgt
     3301 tgatcgcaat aataaaggag agggaacact caagcgttac aagaaaaaat cgttttattg
     3361 gtacaagaag gttattgcaa gtaatggcag tcaaatagaa taaagagcag actaataact
     3421 gttaactctc tttttctcat tcataaaata gtgtataata attgaaagaa attaagatta
     3481 ataaggagat ttcatgaaat ttgaaaaacg gcaggtctat tatgttgtca taacatttgc
     3541 tatttgctat gctatacagg cttattgggg agctgtttct aatattttaa ctacgcttca
     3601 taaggcaata tttccttttt tgatgggagc tggaattgcc tatattatta atattgtaat
     3661 gtcagtctat gagcgattat atataaagct ttttaaagga tctagactat taatggcaat
     3721 caagcgtagt gtttctatga ttttatccta tgcaactttt attggtttaa ttgtctggct
     3781 attttcaatt gtcattccag atttgatttc tagtttgagt tctttattgg ttattgatac
     3841 cggagcactt gctaaattgg ttaataatct caatgaaaat aaacaaattt ctgaggcttt
     3901 aaattatatg ggaacagata aagacttagt ttctacttta agtggttata gccagcagat
     3961 tttgaagcaa gttttatctg ttttaacaaa tttactaacc tcagtttcct ctattgcggc
     4021 aacacttctg aatgtttttg ttagttttat tttttcaatt tacgttttgg caaacaagga
     4081 gcagttggga cgtcaattta atttgttaat tgatacctat ttaggttcaa caggcaaaac
     4141 attccattac gttcgtcata tccttcatca acgtttccat ggtttttttg taagccaaac
     4201 tttagaagct atgattttag gaagtttgac ggttattggt atgttgatct tccaatttcc
     4261 ttatgcttta acagttgggg ttttagttgc ttttacagct ctaataccgg ttgtgggagc
     4321 ctacattggt gttacaatcg gtttcatctt aattgctact gaatcgctta ctgaagcatt
     4381 cttgtttgtt cttttcttga tccttttaca acaatttgag ggaaatgtca tttatccgaa
     4441 agttgtcggt ggatcgattg gactgccttc tatgtgggtt ttaatggcta ttactatcgg
     4501 aggtgcttta tgggggatct taggcatgtt acttgctgtt cctgttgcag ctactatcta
     4561 tcagattgta aaagatcata ttatcaagcg acaaacgctt agaaatcgtg cacgaaccta
     4621 tcgttaaata aaaaatactt agatttttca tctaagtatt ttttattgat ttaaaacatg
     4681 agtttttgat tagtttacga atattatgac tggaggtgtt ttatgtacca tattgaatta
     4741 aaaaaggaag ctttactacc aagagaacgc ctagttgatt taggcgcaga tagattgagt
     4801 aatcaggagt tattagccat tctcttacgt acaggtatta aagaaaaacc tgttcttgaa
     4861 atttcaacgc aaattttaga aaacataagc agtttagcag attttggtca attatcctta
     4921 caggagttgc aatccattaa aggaatcggt caggttaaat ccgtcgaaat aaaagctatg
     4981 ctagaactag caaaacggat tcacaaagct gaatatgatc gtaaagagca aattttaagt
     5041 agtgaacaat tagcgaggaa aatgatgctc gaattagggg ataaaaaaca agaacattta
     5101 gtagctattt atatggatac acaaaatcgt attatcgaac agagaactat ttttattggt
     5161 actgtacgtc gttcagtagc agagccaaga gaaattctac attatgcttg taaaaacatg
     5221 gcaacttctt tgattattat acataatcat ccctcaggtt ctccaaatcc cagtgaaagt
     5281 gatttaagtt tcactaaaaa aataaaacga tcatgtgatc atctgggaat tgtctgccta
     5341 gatcacatca tcgttggaaa aaataaatat tatagttttc gagaagaagc agatatttta
     5401 taaataatga actatttatc gctgctggtt catgaaataa agtaaagttt gtaattcgct
     5461 tgttaaatct acatactgaa caatgatatc ttttggtaat gttaaatgaa ctggagaaaa
     5521 actcaagatg ccttttatac ctgctttgac taagatgtca gcaacttctt gggcttctgt
     5581 actaggtact gttaggatag cagtttcaat atcactatct attaaatggt cattgatagt
     5641 cgaaataccg tagacaggaa ttccatcctc ggttgtttta ccaactaaat cattgctatc
     5701 taaatcaaaa gccattgaaa tttgcatttt attgcgatcg tggaaacgat aatgcaagag
     5761 agctctaccg atatttccac accccaccag cataacattt gttgtagaat gatcgttcaa
     5821 tatttctgca aagaagttca taagtttttt gacatcataa ccaaaaccac ggcgtcctag
     5881 ttcaccaaaa taagaaaaat cacgtcgaac agtagcagaa tcgataccta gggcatctgc
     5941 aatttgtttg gaactagctt tttcgatgcc atcagtatta aaacgtttaa aaatacggta
     6001 gtacagtgat aaacgtttag cagttgcttt aggaatagac ttatcc
//
LOCUS       DATRNX010000112         5964 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRNX010000112 DATRNX010000000
VERSION     DATRNX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..803)
                     /locus_tag="VBC81_001720"
     CDS             complement(<1..803)
                     /locus_tag="VBC81_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6912738.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNH"
     gene            complement(793..2544)
                     /gene="aspS"
                     /locus_tag="VBC81_001721"
     CDS             complement(793..2544)
                     /gene="aspS"
                     /locus_tag="VBC81_001721"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6912739.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(2637..3917)
                     /locus_tag="VBC81_001722"
     CDS             complement(2637..3917)
                     /locus_tag="VBC81_001722"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6912740.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            4137..4319
                     /gene="rpmF"
                     /locus_tag="VBC81_001723"
     CDS             4137..4319
                     /gene="rpmF"
                     /locus_tag="VBC81_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6912741.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            4335..4484
                     /gene="rpmG"
                     /locus_tag="VBC81_001724"
     CDS             4335..4484
                     /gene="rpmG"
                     /locus_tag="VBC81_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6912742.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            4764..5390
                     /locus_tag="VBC81_001725"
     CDS             4764..5390
                     /locus_tag="VBC81_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6912743.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            5402..5740
                     /gene="cadX"
                     /locus_tag="VBC81_001726"
     CDS             5402..5740
                     /gene="cadX"
                     /locus_tag="VBC81_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6912744.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            5791..>5964
                     /locus_tag="VBC81_001727"
     CDS             5791..>5964
                     /locus_tag="VBC81_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912745.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKYNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALT"
BASE COUNT         1930 a         1147 c          957 g         1930 t
ORIGIN      
        1 tcatgattat aagtaccttc agcatcattg atacaggtta caccacgatg taaatcacga
       61 tggatacgtt taatgacgca atcaggctta ctggtaacaa tcatcgcttg catctttttt
      121 tgctttgtaa aaatggcatc tgtaactcta cttgatacaa aaattgtcac catcgagtac
      181 aaagcgtact tccaaccaaa taaaagacct gcaaataata aaataatacc attaataata
      241 aaagaaatac tacctacatc tctgcctgtt ttctttctaa ttgtcaaact gataatatct
      301 gttcccccac tagaaattct agacttaaag ctaaaaccaa caccagctcc cataattaag
      361 ccaccaaaga tagcattgat taagggatca gttgtaaggg tgatttgagg cattagttga
      421 atgaagatag agcttacagt tactgtgata aacgtaaaaa tagtaaattt atgtccaatc
      481 ttacgccaag ataatatcag caaagggata ttaatggcat aaaaagctaa agcaactggt
      541 atttcaaaag aaaaccagtg tttactgaca gctgaaataa cctgagctaa accagttgct
      601 ccacttgaat aaacatgtcc aggttgaaaa aagaaattaa cagcaacact ggatagaata
      661 ccatacagta atgaagccga aattttctct gcatattttt ctcgtgagat actttttaaa
      721 gtgtgtaaga gaccaaattt tttagcccat acactgataa tatattttac ctttttttct
      781 aaaggggttc gtctagtttt cataactttc tacagtaagt gataattctt ctaattgttg
      841 ttctgaaacg agactaggag cttgagtcat tggatcacta gccttattgt ttttagggaa
      901 tgcaataact tctcgaatat tgtccttgcc tgctaacaac ataacaaagc ggtcaaggcc
      961 tattgccaat ccaccatgtg gtgggaagcc gtaatccata gcttcaagta agaaaccaaa
     1021 ctgttcctga gctgattcag cgctaaatcc taaagccttg aacatacgct cttgtgtatc
     1081 tttttggtta atacgtaggc taccaccgcc taactcatat ccattcaaaa caatatcata
     1141 ggcaactgca cgtactttag ccaaatcccc ttccaactca tgcgctgtct ccgctgttgg
     1201 caaggtgaat ggatgatgag ccgacatata tcgtccctct tcttctgacc actcaaacat
     1261 tggccaatca acaacccata ggaaattaaa tttagaataa tcaatgagtt caagttcttt
     1321 tgcaatgcgt gtacgaagcg ctcctaaagt ctcgttagct acttcaagac tatctgctac
     1381 aaatagaatt aagtcattat tctctagctg caaagcttct gtcaacctac cttcaatagc
     1441 tgtcaagaac ttagcaaccg gacctgaaat agtattatct acatatttta gccatgctaa
     1501 acctttggca ccgtattgtt ttgcaatttc tgttaattta tcaatatttt ttcgagagta
     1561 cttgtccgcc ttgtctttga caacaatcgc tttaactact gaagcttctg agaacacctt
     1621 gaagtcaact tcttttacaa tttctgtcaa gtcttgtaaa agcatatcaa accttgtatc
     1681 aggcttatca gacccgtagt tattcattgc atcatcatat gccatccttg ggaatggtaa
     1741 agaaacctct aaacctttag tgtctttcat tactttagca atcataccct caacaatgtc
     1801 ttgaatttct tgatcactaa ggaatgatgt ctctaagtca acttgtgtaa actcaggttg
     1861 acgatctcca cgtaaatcct catcacgaaa acatttgaca atttgataat aacgatcaaa
     1921 tcctgcattc atcaatagct gttttgtaat ttgagggctt tgtggaaggg cataaaaatg
     1981 tccttggttg acacgtgatg gaaccaaata gtctcgtgcc ccctctggag ttgattttgt
     2041 tagcatcgga gtttcgacat caataaactc taagttgtca aggtaattac ggattgagtg
     2101 tgttactttc gcacgtaatt tgaaattttc aagcatttcc ggacgacgaa gatccaagta
     2161 acggtaacgc atgcgtgtat catcatttgc ctcaatacca tctttaattt caaatggtgt
     2221 tgttttcgag gtattaagaa ccgataattc ttgcactttt aattcaactt ctcctgttgg
     2281 taaattatca ttagcttgtt ctcgtgctgt cactacaccg ctaacctcta taacaaattc
     2341 actacggaga ctttctgcgg ttgccataac tgaggctgct acttcttcag gattaataac
     2401 taattgcatg attccctcgc gatcgcgaag atcaataaaa ataaggcctc caaggtcacg
     2461 acgacgacca acccatcctt ttaaagtaat cgatgttcct atgtgctcac tacgaacacg
     2521 cccagcatac atactacgtt tcatctattt tctccattta aaatattcat tccaacctat
     2581 tataacaaaa agcacctaaa aaccccaccc attaaggaga gtttttagga actgttttac
     2641 aatcctaatt gtttaagcac agatgaaaaa tctgttttaa tgttttcaaa actaacttta
     2701 acttcttgac gtgtttgatt attttttaaa ccgacctctt tcgaatctac ttcactgcta
     2761 cctaatgtca taatcacttt agcattaaaa gtatctgcag acttaaattg tgctttgatt
     2821 ttacgaccca aataatcacg ctctacttta aaaccttgat gacgtattga ttgtgccaaa
     2881 tctaaggcag ctagattagc ctctgatcct aatacagcta tatacagatc tatagtattt
     2941 tctattggta aagaaatccc ttgcttatct aatattaaga gcaagcgttc taatcctaat
     3001 ccaaaaccaa atgctggtgt ttctggtcca ccaaaatagg atactaagcc gtcataacgg
     3061 cctccagcac agatcgttaa ttcattatct tcaatctcag tgataaactc aaaaatagta
     3121 tggttataat aatcaagacc acgaaccata ttagtatcaa tgatgtatgg aatatttaga
     3181 gcatcaagca tatgacacac agcatcaaag tgggcttggc ttgactcatc taaataatct
     3241 aaaatagaag gagcattttc aactgctagt ttgtcttctg gctccttaga gtctagtacg
     3301 cgcaatggat tttcattcaa acgacgttga ctatccttag acaattgttc acgtatgggt
     3361 gttaagtaat caataagagc ttggcgatag gcttgacgac tttctggact tcctaaacta
     3421 ttaagatgaa gtttaacgtt tttgatacct aaatcttcaa atagatgatg tcccattgcg
     3481 attgtttcaa catcagttgt tggattattt gatccaaagc actcaacgcc tacttgatga
     3541 aattctcgta aacgaccagc ctgtgggcgc tcatatcgga acattgatcc aatataatac
     3601 attttggttg gtttttgaac ttctggtgca aatagtttat tttcaacata agaacgtaca
     3661 acaggtgctg ttccttctgg acgaagcgta atgtgacgat ctcctttatc atgaaaatca
     3721 tacatctctt ttgtaacgat atccgttgta tccccaactg agcgactaat aacctcgtaa
     3781 tgttcaaaca ttggtgtacg aatttcatca taatgatatt gtttaaagag attgcgtata
     3841 acattttcaa cgtattgcca ctttgctgat tctccaggta agatatcctg agtccctttt
     3901 ggtttttgaa gtttcataag tttacctctt cgattagttt tctctttaaa cattctacca
     3961 ttttttctta tattttaaac aagcatatat catttgcttt agtttgatag tattttcacc
     4021 atgaaattgt aaaaataaaa agttttgtca agtaacttta ctttacaaaa aagatatgtt
     4081 atcatagtga agttgatgaa aatcaaaact tacacatcga aaggaagaat aagaaaatgg
     4141 cagtacctgc acgtcacact tcaaaagcga agaaaaacaa acgtcgtaca cactacaaat
     4201 tgacagctcc atctgtacaa ttcgacgaaa ctactggaga ttactcacgt tcacaccgtg
     4261 tatcactcaa aggatactac aaaggacgta aaatcgctaa agctaacgaa gctaaataat
     4321 agaagggaga taccatgcgc gtaaatatta cacttgaaca taaagaatct ggtgaacgct
     4381 tgtaccttac ttcaaaaaac aaacgtaaca ctccagaccg tcttcaattg aaaaaatact
     4441 caccaaaact tcgtaaacac gttgttttca ctgaggttaa gtaagggttt cagtacacat
     4501 caatagacgt caaaatattg attttaagcg atttttgatt ttttgaaact cattatatcg
     4561 cactacaaat caataaaaac tctacctttt actctacctt ttttagataa atatatcatc
     4621 tggatattga agttaagccc tgctatcatt tcgatggcag ggctttttaa tatttgtaca
     4681 ctaacttctt atctatattt tggtggattt taagttgaca tctacaaagt ttaatgttag
     4741 aatacattca aaaatatatt tgaatgaggt attttatgat tcaaaatgtt gttacttcaa
     4801 taatcctgta ttctgggaca gccgtagact tacttattat cctaatgtta ttttttgcca
     4861 aaagaaaaag cagaaaagac atcattaaca tctatttagg acaatttcta ggctctgtta
     4921 gtctaatatt cctaagtttg ctttttgcat ttgtcttaaa ttatattcct agtaaagaga
     4981 ttttaggttt actcggtttg attccaattt tcctaggcct aaaagttttg cttttaggag
     5041 attctgatgg agaagctatt gcaaaagatg gtttgcgaaa agacaataaa aacctgattt
     5101 ttctagtcgc tatgattact tttgcaagtt gtggcgctga caatattggt gtctttgtcc
     5161 catattttac caccttaaat ttagcgaatt tgatagtgac tttacttact tttctagtca
     5221 tgatttatct cttggttttt tctgcacaaa aattagcaca agtcccttct gttggagaaa
     5281 ctttggaaaa atatagcaga tggtttattg ccgttgtcta tttaggattg gggatgtata
     5341 tcctgattga aaacaacagc tttgacatgc tatgggctgt gttaggctag gagaaaaaat
     5401 tatgaaaaaa gatagtatct gtcaagtgga tgttataaat caacaaaatg ttacaaccgc
     5461 aacgaactac cttgaaaagg aaaaagtcca aaaatcactt cgcattttat caaaatttac
     5521 cgataataaa cagataaata tcatctttta tctccttgcc gtcgaagaac tctgtgtctg
     5581 cgatatagcc tgtttattaa atctcagtat ggcatctgcc tcccaccatc ttcgtaaact
     5641 agccaatcaa aacatcttgg acactagaag agaggggaaa attatatatt attttataaa
     5701 agatgaggaa atcagagatt tttttaatca actaggataa caactatttc tactactttc
     5761 tacttttcca tgattatagg gggattatat atgaaatttt ttgaagaaaa ttattcacaa
     5821 gaaataccta ctcgtatcaa aaatttaaga aaaaaataca atattacaca aagcgaacta
     5881 ggcaatgcag gtcaagttag tcaagttgaa agtggtaagc gaccaattac gagttcgatg
     5941 ttagtttatt taaatgctct aacc
//
LOCUS       DATRNX010000113         5863 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRNX010000113 DATRNX010000000
VERSION     DATRNX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            58..714
                     /locus_tag="VBC81_001728"
     CDS             58..714
                     /locus_tag="VBC81_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912746.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            711..1484
                     /gene="fetB"
                     /locus_tag="VBC81_001729"
     CDS             711..1484
                     /gene="fetB"
                     /locus_tag="VBC81_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6912747.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            1648..2466
                     /locus_tag="VBC81_001730"
     CDS             1648..2466
                     /locus_tag="VBC81_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6912748.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(2503..3387)
                     /locus_tag="VBC81_001731"
     CDS             complement(2503..3387)
                     /locus_tag="VBC81_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6912749.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGVRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            3580..4638
                     /locus_tag="VBC81_001732"
     CDS             3580..4638
                     /locus_tag="VBC81_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6912750.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            4652..5644
                     /locus_tag="VBC81_001733"
     CDS             4652..5644
                     /locus_tag="VBC81_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6912751.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
BASE COUNT         1882 a          858 c         1124 g         1999 t
ORIGIN      
        1 atttttatgt tagacttaat tataatgatt ataaataaga aataaaggag cttaacaatg
       61 tcctatttaa cctttaaaca cgttgatttt aaaacggatg ataagttggt tttaaatgat
      121 atcaactttg caattgatga aggtgatttt gtttctattg tagggccatc tggaagtggt
      181 aagagtactg tattaaaatt agccagtggt ttaatgagtc ctactgcagg tcatattttc
      241 tttgatggaa aagatttgaa ccaattagaa ccgattgaaa gtcgaaaaat gatttcttac
      301 tgttttcaaa cgccacattt atttggtaat acagtggaag acaatattag ttttccttac
      361 cacattcgac acgaaaaagt agattataga cgcgttgatg atttatttca acgttttgaa
      421 atggaccagt cttacctgaa acaggatgtc aaaaaactat caggaggaga aaagcaacgt
      481 attgctctga ttaggcaatt attgtttgaa cctaaagttt tactcttaga tgaagttact
      541 tcggctttag acaatcataa taaagctatt gttgaaaagg ttattaaatc attacatgac
      601 aaaggaataa cgattttgtg gattacgcat gatgaggaac agagtcgtcg atttgccaat
      661 aaagtcttaa aagttgtcaa tggtagtatt gaagcaatgg aggttataaa atgagcggtg
      721 caaattctat ttctttaatg tcattactaa tggcatcgtc attagttttg attactttat
      781 ttttttctta ctggcaaaag ttaaacctag aaaaagaagt gattattagc gctatccgag
      841 cagtgattca attattagca gtgggttttt tgctagacta tatatttggt tatcaaaatc
      901 ctattttcac agctctttta atgctattca tgattattaa tgcttcttat aacgcagcta
      961 agcgtggtaa agggattaat aaagggtttg ttatttcatt tatagctatt gggtcaggga
     1021 ctataatcac tttatctgtc ttgatttttt caggaatttt aaaatttgtt ccgaatcaaa
     1081 tgattcctgt aggtggtatg attattagta attctatggt agcaatcggt ttgtgctata
     1141 aacaattatt gtccgaattt agaagtaaac aagaggaagt ggagacaaaa ttagctttag
     1201 gagcagatat tttgccagct tcgattgata ttattcgtga tgttatcaaa actggtatgg
     1261 tacctacaat tgattcagct aaaacacttg ggattgtatc tttgccaggg atgatgacag
     1321 ggttaattct tgctggaact tcacctatcc aagcggttaa ataccaaatg atggtgacgt
     1381 ttatgctatt agcaacgact tcgatagctt cttttgtagc tacttatttg gcttataaaa
     1441 ttttctttaa taatcgtaag caattagtag taacaaaaga ttgattcatt ttaataaaca
     1501 gtatgttagt ctggtaagta tagtccaggc ttttttggta tcttaatctt atttttgatc
     1561 gaaaattgac caagttattg agtcgattac gtttaaatgc tactatttaa tagaaattgt
     1621 tgtagggatt atcaaaagga ggaagaaatg attttaaaaa tttgtcgtgc agcatatagt
     1681 ttacaatggg gaggtgttta ccaattagct ttgctggatt atcctcgaat taaggcgttt
     1741 gaattggaaa ggataggagc tttcatagct tacgagaaac aatataaaag aaaaactgag
     1801 atacaatgtg acgataaaca tctcctcgca aaaattgttc attttttaaa atacaatagt
     1861 tttacttttc cctatattcc caaatataga gaagcggcag ctacttttaa tgaggatggt
     1921 attagtttaa cttctgattt tttaagccat acatgtacga ttgaaactgc aaaactaatt
     1981 tttaaagaag gtaaaatctt atcagcagtt aaagccttta ataagcctgc tgaagtactg
     2041 gtaaaagata agaggaatgc tgctggagac cctaaagatt actttgacta tgtgatgttg
     2101 aactggtcaa ataccaattc tggttatcgt ttagtaatgg aaagattgtt aggcaaagca
     2161 ccatctgaac aggagttaac agtaggtttt aagccagggg tcagttttca ttttacttat
     2221 caagatatca tcaatcatcc tgattctatt tttgatggtt atcatcctgc taaaattaaa
     2281 aatcagcttt ctttagcaga acatttagtt gcatgtgtta tcccaaaaca ttatcaagaa
     2341 gattatcaaa gccttgtgcc caatgacttg aaacacaggg tttattattt agattactgt
     2401 aacgaaacac tttatgagtg gaatcaaaaa gtttatgatt ttctttgtca tttggaaaat
     2461 aaataaaacc aaaaagttta aaactttttg gtttaactgc tattaattgg tctttaaaag
     2521 acttaaaaat tcttcaatca taggactaaa agaagtatca tttcggtaag caataccaat
     2581 cggataaaaa ttatttttat ccttaagtgg gacccagcat aaatcttgaa cttggctaat
     2641 gctttcaaat tcacttggta aaatagtgac tgcattgagg tcttgtaaac tatgaataag
     2701 aacttcccag tcattgtgtt taaagacgat atgggggtca aatcctaaag cacgacattt
     2761 acgaggcaac atctctccta gcatataatg ttcgttgagt gaggcaactt tataatctct
     2821 taaatcatta agttcaattt caggaagtgt tgcaagagga tgcgattttg gcatgacaat
     2881 gcttacttta tatcctttag ttgaggtttg cagtggttcg atagtgatat cattgcgggt
     2941 acttggaaag gacaataagc caatatcgat ctttcctttt gctaacaatt cttgtaattt
     3001 acgggaacca tcttgaatca aagatacttc aacatttgaa tgtgtagcca taaaagttga
     3061 aatctgtttc ataaattgaa tagcaaaaag agaagtaagc cctacacgga caccttcctt
     3121 tttttcttga tttaattgct ggatctccgt aaccatttga ttaacattag tcatcaacaa
     3181 ttgtcctttc tcatagagaa tttctcctgc ttcagttaaa gcgagttgac gaccattttg
     3241 taagaataaa atagtattta attctgcttc taatttttta atggattgtg aaagtgtagg
     3301 ttgggtcaca aaaagacttt ttgcagcgcg tgaataattt tttgcttctg caacagcaat
     3361 aaagtaagta agttgtctaa tatccataat atctgtcctt ttttcttagt aatagtatag
     3421 cacaagtcta tcaatctgac ctttatgatt atttccacaa taatattagt aaaagctata
     3481 cttatataat tttttccaat tttccttttg caaatagcta tgatatagtt aatatagtta
     3541 gtgaaatcac aaacaatcat tattagtagg aggcatctta tgtcggatgt tgtcgaaaaa
     3601 caaacaacta aatcgtttat aatgaacgta ttaaatggtt tagctttagg gactgttatt
     3661 gtcttgatac caggagcaat ccttggagaa ttaatgaaag ctttactgcc aatgtggagc
     3721 ggctttgcaa ctttaatcgc tgcaacagca gttgcaacaa gtatgatggg gcttgttatt
     3781 ggtattatgg ttggtctcaa ttttaaattt aatcccattc aatcagcatc actaggatta
     3841 gcggttatgt ttgcaggtgg agctgcaacc ttccttaaag gcgctattat gctaaaagga
     3901 acaggcgata tcattaacat gggaattact gctgccttag gtgtcctttt gatccaattt
     3961 ttatctgaca agacgaaatc attcactttg attgtcattc caacagtaac actcttacta
     4021 gttggaggag taggtcatgt gttattacct tatgtcaaaa tgattaccac catgattgga
     4081 caagggattg cttcattact tggtttacaa cctgttttga tgtcaatttt aattgcgatg
     4141 attttctgtt tcttgatcgt atctccaatc acaacagttg ggattgcttt ggcaattagt
     4201 ttgtccggaa ttggttctgg tgctgctaat ttaggtatct gtgctgctag ttttgggctt
     4261 tgtatggctg ggtggtcagt caattctaag gggacagcct tagcgcatgt tttagggtct
     4321 cctaagattt cgatggctaa tgtattagca aaaccgaaga ttatgctacc tatgatatct
     4381 tcagcagcaa tactaggaat tctcggagct ctctttaata ttcaaggaac accagctagt
     4441 gcaggttttg gtatcagtgg tttgattgga ccgattaatg ctcttaattt agcaaaaggt
     4501 ggatggtcag ttatgaatat gttattgata attataatat ttgttgctgc accaattata
     4561 ttgaatttta tttttaatta cctctttata aaagtactta aaattattga tccaatggac
     4621 tataaattag atatttaagg agatattagt gatgaaactc aaagtattta atgttagaga
     4681 agaagaagca acattagcac aagattgggc aaaccgaaat catgtggaac tttctatgag
     4741 tgaaggacct ttgacattgg aaactgttaa tgaggtagaa ggttttgatg ggattgctaa
     4801 tgcacagatt gagcctttag atgatgctat ttatcctctt ctcaaagaaa tgggaattaa
     4861 gcaaatcgct cagcgcagtg ctggtgttga tatgtataat cttgagttag ctaagcaaca
     4921 tggtattatc attagcaatg tgccaagtta ctcaccggag tctattgcag aatttacagt
     4981 gacaatcgct cttaacctta ttcgaaaagt tgagcttatt agagctaatg ttcgtgaaca
     5041 gaatttttca tggaccttgc caattcgagg acgagtccta ggtaacatga ctgtggctat
     5101 aataggaact ggtcgcattg gtctagcgac tgctaaaatt tttaaagggt tcggatgtcg
     5161 tgttattggt tatgatattt accataatcc aatggcagat ggtatccttg agtatgtcaa
     5221 ttcggtagaa gaagctgttg aagaagctga tcttgtgtcc ttacatatgc caccaactgc
     5281 tgaaaatacg catctattta atttagatat gtttaaacag tttaaaaaag gtgctatttt
     5341 aatgaatatg gcacgtggag cattggtgga aaccaaagat cttcttgagg ctttagatca
     5401 agggcttttg gagggagctg gtattgatac ttatgaattt gaggggccat atatacctaa
     5461 aaattgtcaa ggtcaagaca tttcagacaa ggatttccta agactgatta accatccgaa
     5521 agtaatatac actcctcatg cggcttatta tacagatgaa gcggtcaaaa atctagtaga
     5581 gggtgcctta aatgcttgtg tagaagtgat tgaaacggga acaactacta cgaaagtcaa
     5641 ctaaaagatg cttaggcatc tttttttgtg gggtaacaaa acatatgatg attatgacct
     5701 ttaaagcaaa aaaacatctt atgtatttat agcttgaaag tcttttttat aataaaaaaa
     5761 gatatttttt ataaaaatat tgaaaaaaac atcagatgaa tagtattatt taatcataaa
     5821 agcgtttaca aaatacaagg agggttctct catggagaaa aca
//
LOCUS       DATRNX010000114         5684 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRNX010000114 DATRNX010000000
VERSION     DATRNX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            37..1905
                     /gene="glgB"
                     /locus_tag="VBC81_001734"
     CDS             37..1905
                     /gene="glgB"
                     /locus_tag="VBC81_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6912752.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            1947..3086
                     /locus_tag="VBC81_001735"
     CDS             1947..3086
                     /locus_tag="VBC81_001735"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6912753.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            3076..4209
                     /gene="glgD"
                     /locus_tag="VBC81_001736"
                     /pseudo
     CDS             3076..4209
                     /gene="glgD"
                     /locus_tag="VBC81_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            4206..5636
                     /gene="glgA"
                     /locus_tag="VBC81_001737"
     CDS             4206..5636
                     /gene="glgA"
                     /locus_tag="VBC81_001737"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6912754.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
BASE COUNT         1816 a          829 c         1190 g         1849 t
ORIGIN      
        1 gtcttatatt gactagtaga cctgtgaggt gagtagatgt ccaataaaga tgagttatat
       61 acttttggaa taggtgaaaa ttttcatctc cagaattatt taggcgtaca tagcgaaaat
      121 ggaagctttt gtttcagagt ttgggctcct aatgctgaaa atgttcaagt tattggagat
      181 tttacagatt ggcgaaatcg acctctccaa atgaacaaaa ttcaagcagg agtttgggaa
      241 gctaacagtt tggatgcgcg cgaaggtgat ttatataaat atttggttac tcgtaaaggt
      301 ggtcaagtag ttgagaaaat cgatcctatg gcagtttata tggaaagacg tcctggaact
      361 gcaagtgtta ttaaagttct tagaaataaa aagtgggaag atggtctttg gatgggaagg
      421 cgcaaacgtc tgggttttca gaagcgtcca attaatattt acgaagttca tgctggttca
      481 tggaaaaaag atgattttgg acatcctatg actttttctc agttaaaaga ttacttaatc
      541 ccctaccttg ttgagatgaa ttatactcat gttgaattta tgcccttaat ggcacatccg
      601 cttgatatga gttggggata tcaattaatg gggtattttg cttttgaaca tacctatggt
      661 actccagaag aatttcaaga ctttgtagaa gcatgtcata aaaataatat aggagttctt
      721 gtagattggg ttccaggaca ctttattcag aatgacgatg ctttagccta tttcgatgga
      781 actgctactt atgagtatca aaatcatgat cgtgcgcata actatcgatg gggagctctg
      841 aactttgatt taggaaaaaa tcaagtacaa tcctttttga tttcgagtgc cttgttctgg
      901 attgaacatt atcatattga tggtattcgt gttgatgcgg ttagtaatat gttatatctc
      961 gattatgatg aaggaccatg ggaagctaac caatttgggg ataatcgtaa tttggaagga
     1021 tatcactttt taaggaaact taacaaagta attaaagagc gtcatcctaa tgttatgatg
     1081 attgctgaag agtcaacagc ttctacccca ataactaaag acttagagtc tggaggattg
     1141 ggttttgact ttaaatggaa tatgggctgg atgaatgata tcctgcgttt ttatgaagaa
     1201 gatcctctct ataggcaata tgattttaat ttagtgactt ttagttttat gtatatattc
     1261 aatgagaact ttgtactagc tttttcacat gatgaagtag tacatggtaa aaagagtatg
     1321 atgcataaaa tgtggggtga ccgttataat caatttgctg gtttacgtaa tttatatgca
     1381 tatcaaatgt gtcatcctgg gaaaaaactt ctctttatgg gaagtgagtt tggtcaattt
     1441 ttagaatgga aatataatga tcaactagaa tgggaaaact taaatgatga tatgaaccaa
     1501 aagatgcaac gctatactaa acaattgaat caattttata aagatcataa gtgcctatgg
     1561 cgtattgatg attcctttga cgggctagaa attattgatg cggataataa atctgaaact
     1621 gttttatctt ttatacgaaa agatgataaa ggcgatttgt tactttgtgt ctttaatatg
     1681 actcctgtag aacgccctaa ctttactatt ggtgttccgc aagcgggtat ttatgaagaa
     1741 gttttaaata ctgaaatgga agagtttgga ggtgtttgga aaaaccacaa tcctgtaacg
     1801 aaaacacaag tggctacatg gaaagattat gatcatactt taagttttac attaccagcc
     1861 cttggggcaa gtgtttggag aataaaacgt cggcttcgta aataagtctg caatttagta
     1921 acctcatttt gaaaggaaag aataatatga aaaatgaaat gttagcactc attttcgtcg
     1981 gaggacaagg cactcgactc ggaaaattga ctcagagcat tgcgaaacct gctgttcaat
     2041 ttggaggtcg ttaccgtatt attgactttg cgctttcaaa ttgtgcgaat tcaggtatta
     2101 ataatgttgg agttattact cagtatcaac cgctggaact caatactcat attggaaacg
     2161 gatctagctg gggactagat ggtatcgatt cgggagtaac tgtattacag ccatattctg
     2221 caacagaagg taatcgctgg tttcaaggta ccagccacgc tatctatcaa aatattgact
     2281 acattgatcg tattaatcca gaatatgtac tcattctctc tggggaccac atctataaaa
     2341 tggactatga tgacatgcta cagacgcata aagataattt agcaagccta acagtcgctg
     2401 ttttagatgt tcctttaaaa gaagcaagcc gttttggaat tatgaatact gattcaaacg
     2461 accgtattgt cgaatttgaa gaaaaacctg aacatcctaa gtcaacaaaa gcttcaatgg
     2521 gaatatacat ttttgactgg aagagattac ggacagtttt aattgatggt gaaaagaacg
     2581 gcatagacat gtcagacttt ggtaaaaatg ttataccagc ataccttgaa tcgggtgaac
     2641 gtgtttatac ctataatttt gatgggtatt ggaaagatgt tggtactatt gaatcccttt
     2701 gggaagctaa tatggaatat attggagaag ataacaaatt acatagtcgt gatcgttcat
     2761 ggaaaatcta ttctaaaaat ttgattgcac cacctaattt catgacagaa gatgcaaatg
     2821 ttaaagattc tttagttgta gatggatgct ttgttgcagg aaatgttgag cactccattt
     2881 tatcaactaa tgtccaggta aaaccaaatg ctattattaa agactccttt gttatgagtg
     2941 gcgcgactat cggtgaaggt gcaaagataa atcgagctat tatcggtgag gatgctgtta
     3001 ttggtgatgg tgttgtgatt gatggaagta aagaagtaga agttattggt tataaagaag
     3061 tagcgggggt tccaaatgaa gattgataaa tatactgcga ttttaggaaa tactgttagt
     3121 tacccagata tgcacagttt gacggataat cgtccgcttg cttctctacc ttttgatggt
     3181 aagtatcgtt taattgattt tcaattatcc tctttagcta atgcaggcgt tagaagtatt
     3241 tatggtattt ttcgtgggaa taatgttcgc tctgtttttg accatattag aagtggtcgt
     3301 gagtggggct taggtacact gttgagtcac tattctttag gattctatga tacagacgat
     3361 gatactcgta cagtcaatgg ggattattac gattaaattt taacttactt gaggcgttcg
     3421 ggtagcgacc agacgatata tatgagttgt gatatgcttt gtaatattga cttaagtcaa
     3481 gtaatccact tgcataatgc aaatggtgtg caagccaccg tggtttataa aaaaatgccg
     3541 agatcagcta ttgcagaggc taatcaagtc ttgaccattg atgaatctga tcatgtagtg
     3601 agtctaggta aagctgtaga taatgacgat aaccaaaaaa tgtctgctga gatttatgtt
     3661 atcgatacac cttggttaat cgaaaaaatg gaagaggaag ctcaaaataa tgaaccacgt
     3721 aaactccgct tcttacttcg tgatttaatt gttgaaagta atgctttggc ttttgaatac
     3781 actggttatt tgtcaaacat ttcttctatt aaatcctatt atgatgctaa catggacatg
     3841 ttaacaccaa ataaatttta ttcgctcttc ttttcaaacc aaaaagttta tactaaggtg
     3901 aaaaatgagg aagctactta ttttgataaa caatcaaatg tctctaattc gcaactagcc
     3961 tatggaagta ttattaaagg ttatttagac cattctatcg tttctagaaa ttgtctcctt
     4021 gaaaaaggga ctcgagtagt caatagtatt atctttccaa aagtaaaaat cggagaaggg
     4081 gcaaccattg aaaatactat tattgacaaa tgcgttaagg ttgcatccgg agttacattg
     4141 aaaggtagtt tagataaacc gttagtgatt cctaaatttt cagagattaa tgaggatatt
     4201 atacaatgaa aataatgttt gttgcggcag agggtgctcc ctttgctaaa acaggtggtt
     4261 tgggagatgt gattggcgca ctcccaaaat cacttagtaa aaaaggtcat gatgtcgctg
     4321 ttgtcatgcc atattatgat atggtagacc aaaaatttgg agaccaaata gaaaacctca
     4381 tgtatttcta tacagatgtt ggctggcgtc atcaatatgt aggagttaag agactttctc
     4441 aagataatgt tacgttctac tttattgata atcaatatta tttttataga ggtcatgtct
     4501 atggtgattg ggatgatggg gagcgttttg cttatttcca attagctgct ttagagttga
     4561 tggagaaaat agattttatt cccgatgtct tacacgtcca tgattatcat acagcaatga
     4621 ttcctttctt attgaaagag aagtaccact ggattcaggc ctataataat ataagagcag
     4681 tttttactat tcacaatatt gaatttcaag ggcagtttgg tccagaaatg ttaggagatt
     4741 tatttggagt tggcgctgag agatatgaag atggaactct tagatggaat aattgtctta
     4801 attggatgaa agcagcaatt ctttactcag atagagtgac aacggtatca ccaagctacg
     4861 caaatgaaat aaaaactcca gaatttggta aaggtttaga ccaaattatg cgtatggaag
     4921 caggtaaatt atctggaatc gtaaatggta ttgacagcga tttgttaaat ccagaaacag
     4981 atgctttttt gccatatcat ttttcaaaaa gcaacttaga gggcaaaatt aaaaataagt
     5041 tggctttaca ggaaaatttg ggcttgcctc aagataaaaa tgtaccatta attggtattg
     5101 tttcacgact taccgatcaa aaaggatttg atattattgc ttctgaattg gacaatatgc
     5161 ttcaacaaga tattcaaatg gttattttag gaacaggcta tcaccatttt gaagaaacgt
     5221 tctcttattt tgctagtcgt taccctgaaa aactatctgc taatattact tttgacttag
     5281 gattagcgca acagatttat gcggcttcag atatcttcat gatgccaagt gcttttgaac
     5341 cttgtggtct ctcgcagatg atggctatga gatatggtag cttacccttg gtacatgaag
     5401 ttggaggatt aaaagatact gtggtagcct tcaatcaatt tgatggtagt ggtacaggtt
     5461 tttcatttaa tcatttttca ggctattggt taatgcaaac tctaaaacta gcattagaag
     5521 tttataatga ttatcctgaa gcgtggaaaa agttacaatg gcaagctatg tcaaaagatt
     5581 tttcatggga tacagcatgt gttgcttacg aacaactgta tcagcaacta caataatcac
     5641 tttattttag tgattagttg ataagcttat cgaatctcta tgct
//
LOCUS       DATRNX010000115         5425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRNX010000115 DATRNX010000000
VERSION     DATRNX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(37..1056)
                     /locus_tag="VBC81_001738"
     CDS             complement(37..1056)
                     /locus_tag="VBC81_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6912755.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            1160..2287
                     /locus_tag="VBC81_001739"
     CDS             1160..2287
                     /locus_tag="VBC81_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6912756.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIEIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2324..3310)
                     /locus_tag="VBC81_001740"
     CDS             complement(2324..3310)
                     /locus_tag="VBC81_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6912757.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCSGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3473..3779
                     /locus_tag="VBC81_001741"
                     /pseudo
     CDS             3473..3779
                     /locus_tag="VBC81_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3937..5310)
                     /locus_tag="VBC81_001742"
     CDS             complement(3937..5310)
                     /locus_tag="VBC81_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912758.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT         1906 a         1100 c          795 g         1624 t
ORIGIN      
        1 taaataaggt tgagatgtac atctcaacct tatttattat tttctaaaaa tcgctttaga
       61 tatgatatag gtcattataa ttgacaaaag aattgtggta gctaatgcta acagttgaac
      121 tggcatatgt gataatttaa agagaccaac tcctaaactt cccaacaatc ccccaatacc
      181 attcattcca aaggaggaaa cgacatcatc aatggcaaaa gctttcgcaa cgtaatccgt
      241 tacaaagtag gtgaagaggg atgcaaagaa tgttacagct aataattgcc aagttaagag
      301 atacccaact cctgctgtac tcgtcacaag accaacaata atgccattca ataagcttcc
      361 tgttttctct tcctcaccat tccacttagc agccaatgtc cacgccaaac caccacctat
      421 aatagcaaat atagtattta aaagtatcat aatggcttgt gaattccact caccaactgg
      481 acccatatta aatccaaacc aaccaaatgt aataagtatc attcctaaaa agagactatc
      541 atttttaacc ttattgtgct gatgttcact cttgccaata acatgagcta aaatatacga
      601 agaaagccct gcagataaat gaacgaccat tccgcctgaa aaatcaagta ctcccatctt
      661 tgcaaaaaca ccctctgaat tccaaattaa ataagctaag ggcgtataca ccaagaaaag
      721 ccaagaaaca acagctaata aaagtcgctt cgtctgaact ctatcaataa ttgacccaat
      781 aagcattact actgcgtaaa gggcgaaaca aagttgaaat aaacaattta caatttgttt
      841 accatctgct tcttggaatt gaaagacagt tttaaaatga ccttcaaatg ctaaaaaata
      901 ggcacccatg aaccaagctg ttgtcgttag caaaacagta agtacaaact gataaataat
      961 ccttgaagtc aatgattgat gtagcgaccc aaagtaatag aatgcaaccc caaaaatcat
     1021 gagccacatc gataaaatac ataatagtaa aaacacaaat agtcctttct ttacaaacac
     1081 tcttttattt gttaaactgt attgttgtta actggttaag tattttatct tacacattta
     1141 tttttgtcaa ggagaagaca tgaaacatta tcaaaccata attatcggtg caggcgctgc
     1201 aggaatcggt tttggttctg ccatgcaacg attaggactt acaaattttc ttatcattga
     1261 aaaagggcac attggagaat cttttttaag atggcccaga acaacacaat ttatcacacc
     1321 ttccttcact accaatggct ttggttttcc agatctaaac gctgttattc cagatacttc
     1381 tcctgctttc tcatttgaaa aagaacatct ttctggagtg gaatatgcac gctacttaca
     1441 attagttgct gctcattata atctccctat ccaaaacgaa acatctgttc tatctattga
     1501 taaacgagat agcctttttg ttattaaaac ttcaaaggga gatttttctg ctgattatct
     1561 tattatggca acaggagaat ttcaaaatcc aaataccatt gagattaaag gtgcagatct
     1621 aggaatgcac tatggtcaag ttgataattt ccatatcaaa tctgacaatc catttatcat
     1681 cattggtgga aacgaaagtg cttgtgatgc actaacacat ctagtatatt tgggaaatca
     1741 agtcgaatta tatacagata cgtttggtcg taaagagagc aatcccgatc caagcatctc
     1801 tttatccccc ctaactaaag aacgtttaaa acatatacaa gaccacaaaa aagaatatta
     1861 tagtatctct gaaggcaaga aagccattga gattaaacaa attggaaaac aatatcaagt
     1921 tacttttgat gacggtagta ctgcggagtc tttccataaa cctattcttt ccacaggttt
     1981 tctaaatacc tgtcatctca ttgacggaat cgctttattt gaatacgata aaaatcagtt
     2041 gccaatagtt actgaagatg acgaatctac tatcgttaat aactgtttct taattggtcc
     2101 aagcttacga caaagagata ctattttctg ctatatttat aaattcagac agcgctttgt
     2161 ccctgttatt aatcatatcg cacaacgaga agggattata ttagacgaag atacccttga
     2221 attctttaaa gaaaatcaaa tgtaccttga taacctagct tgttgcgatg tcaattgtga
     2281 ttgctaaaaa atcgctagct ttattgctag cgatttttta atattaaata tttgctactt
     2341 gaggtaagga ttctccaata gcttctaaac gttctgccca ttcttgacgt gataaaccat
     2401 tttcttgcac atagcgatta cgttcatgtg cacgacattc aggcgagcac cctcgaacat
     2461 acttcgcttc gttttcttct gaagcaaaaa tttgtttgtt acaaaacgga tttgcacaat
     2521 tgacataacg ttcacaaggt gttccatcaa aataatcttt actaataaca gttggattaa
     2581 catgattaat tggcaccgag atacgatcat caaaaacata catcgcacca tcccaaagtt
     2641 caccttgaac ttccggatct tttccatatg ttgcaatacc accgtgtaat tgtccaacat
     2701 ctttaaaacc ttctcgaacc atccaacccg agaatttctc acaacgaaca ccaccggaac
     2761 agtacacaac aacacgtttt tccataaact tatctttgtt gtcacgtacc cattgtggta
     2821 actcacggaa attgcgaata tcaggacgaa ttgctccacg gaagtgacct aagtcatact
     2881 cataatcatt acgcgtatct aaaacaacag tatcttcatc aagcaaggct tctttaaatt
     2941 gtttaggatt taaatactca cctgttgttt ccaaaggatt aatatcgcta tcaaagttat
     3001 tatcttctaa cccaaggtgc acaatctctt tcttataacg aacaaacatt ttacggaaag
     3061 cttgttgatt ttcctcgtca atcttgaacc aaagatcagc aaaacgctca tcgctgtgga
     3121 tccaatccat atacttctga gttgtttcat aatctccaga aacagttcca ttaatcccct
     3181 catccgcaat caaaatacga cctttaagcc caatagattt acaaaattct agatgtttcg
     3241 cagcgtactc ttccgcattt tcaattgaaa catatttata atagagtaaa actctgattt
     3301 tttctgacat tttttctcct catctgcaaa ataagttagt atacttcatt atataacgac
     3361 tccttacaac atagcaaaaa atacgcttag gacatattct ttgctttaaa gacaaattat
     3421 tcttcttaag aacacacata tgttaaaata gtaaaagact aggaggactt ttatgactta
     3481 tgaattatgt ctagaatacg gtacttatcc acttagacct gtagatgctt gggctgatga
     3541 aatcaatact gcacctgcgt ttattactga agataaaaaa acttttagaa cttttagaag
     3601 aagtcaatac cttattccat gagctctttt taacgataga atgttccttc cactatattg
     3661 gtcatgactt cccagaaaaa agagcaaaaa tcacacaaat ttatcatgtg attatcgaac
     3721 atttaagcat acactaccct gaatatgaca ttaaaattga atcattatta atggggtagt
     3781 tttattttaa ctaggtagaa actatctatt aaaaacaatc gatatcacta attaattcaa
     3841 caaatagatt atctaaaaca aaaaaggtct ctttcaagag gccttttttc ataacttatc
     3901 tcactaaata ttaaatgata cttttaccaa ataatatcac caatactcta gactcttatc
     3961 tcttgaaagc aaggtacaga caatagccgc aaatccagaa ataccaaata ttataaatgc
     4021 tactccccaa ccaaagtggt caataataat accaatagct agagtagcga tcgttgctcc
     4081 tccgacataa ctaaatgtgc cagtcaatcc tgaagctgct ccagctgctg cttttggaac
     4141 taactccata gcatacaggc caaccatcat aacaggaccg tagattgatg cccccaaacc
     4201 tagtaaaata atgaagtcta atgccatata ttgaggtcct gctggaataa accaataggc
     4261 agctagtaat aagattgcta caactaagaa ggctacattg gttaaaggtt tagagttttt
     4321 aaacactttg gcagatagat atcctgctgt tagcatccca ataataccac ctatttcaaa
     4381 aagtgaaaaa ccacccatac cagctgttgc agtaatacct tttccaccat cttgcacact
     4441 cgtcgctaaa aacttaggag tccatgagac aataccgtaa cgtaaaatat atacaaatat
     4501 tgaatacaag gatgcccaca tcagtttagt atttggtaga atatattttt ttataatttc
     4561 gcccataccc aaaacttcag gctcttctaa aatgtcactt gaatccacaa cattagtata
     4621 ttgctcttta tgatactctt ctataggcgg aagtccgata gattctggag tatcttcttg
     4681 taaaaagtaa acaagaacag ccaaaagaca agccaccacc cctggaaacc aataagcagc
     4741 acgcgcttga ttaagagaat cattaccagc aagagctaga cctacaccag ttaaaatcgg
     4801 cgctattgct ccaccaaaat tgtgagataa attccaaatg gtagcatatt taattctttc
     4861 tttattatca taccaataga ctaatgtacg cgctccagga gggtatccca tcccttgtaa
     4921 ccaaccatta ataagataca tgatagtaaa gagccagata ctagtaatta cagatggaat
     4981 aacaccgatt aacacattaa ctagtcctgt aagaaacaaa ccaactggaa ggaaaatttt
     5041 agaattcgat tcatctgaaa tcaaaccaga aataaattta gagagaccat aagcaatgta
     5101 gctagcacag gtaataatac caatctcagt agcagacata ttaaggatac cagataaaat
     5161 tgaagtcgta ttacgaacaa aatagtatcc catataacct acaaaaatag aaataaggac
     5221 ttgtacacga taacgaggat atttatgctt aatttcctca gctgataaac gttctctttt
     5281 ctgagggggt gcaaatagtt ttttcaacat agcaaattac ctttccgatt tttagatgtg
     5341 tttaattata caactatgaa ttcacactgt caattaatta ataatttatt ttttatcatt
     5401 ttatcttatt tttttaaatg tggat
//
LOCUS       DATRNX010000116         5409 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRNX010000116 DATRNX010000000
VERSION     DATRNX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            45..1484
                     /locus_tag="VBC81_001743"
     CDS             45..1484
                     /locus_tag="VBC81_001743"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6912759.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRTFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            1486..2448
                     /locus_tag="VBC81_001744"
     CDS             1486..2448
                     /locus_tag="VBC81_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912760.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(2532..2966)
                     /gene="nusB"
                     /locus_tag="VBC81_001745"
     CDS             complement(2532..2966)
                     /gene="nusB"
                     /locus_tag="VBC81_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6912761.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(2959..3348)
                     /locus_tag="VBC81_001746"
     CDS             complement(2959..3348)
                     /locus_tag="VBC81_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6912762.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(3437..3997)
                     /gene="efp"
                     /locus_tag="VBC81_001747"
     CDS             complement(3437..3997)
                     /gene="efp"
                     /locus_tag="VBC81_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6912763.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(4925..5224)
                     /locus_tag="VBC81_001748"
     CDS             complement(4925..5224)
                     /locus_tag="VBC81_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6912764.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT         1781 a          959 c          876 g         1793 t
ORIGIN      
        1 ttactatata gttattagat attaagacag tcaaggagta tcaaatgaat ttacccactg
       61 aaatccgcta tcgtccttat gatgaatgga ctgaagaaga taaggaaaat attttaaaga
      121 atgtctcaaa atccccttgg agagcaactt atcacttaga agctaaaaca gggcttttaa
      181 atgacccaaa tggcttctct tactttaatg gaaaattcca tcttttttat caaaattggc
      241 cctttggagc tgcacatggt ttaaaacaat gggtacatac agaatcagat gatttagttc
      301 atttcaaaga aacaggaatc aagcttaaac ctgaccatgt caatgatagc catggtgcat
      361 actccggttc tgcattagcc attgacgata agctttttct tttttatact ggtaatgtcc
      421 gtgacatgaa atggaaccgt gacccccgcc aaattggagc atggatgaca agtgatggca
      481 agattacaaa atttgataaa gtccttattt cacaaccaaa tgatgtcact gagcattttc
      541 gtgatcctca gattttcaat tatgataatc aattctatgc tgttatcggg gcgcaaaata
      601 gtaaaaaatg tggattcatt aaattatata aagcacttaa taatgacatc catcattggg
      661 agtttgtggg agatttagat tttggtggta ctggatctga gtatatgatt gaatgtccaa
      721 atattatttt tgtcaaagga aaacctgtat tactttatag tcctcaagga ctagataaaa
      781 acgaattgga ctaccaaaac atttatccaa atacctacaa aatcggacaa tatttcgatg
      841 ctaactcatc taagatagtt gaaccatccc ctatctataa tttagattat ggttttgaag
      901 cctatgctac tcaaggattt aatacttctg atggtcgtac ctttatcgtg agttggatag
      961 gcttgccaga tattgattat cctagcgatc aatttgatta tcaaggagct atgagtttag
     1021 ttaaagagtt aagcataaaa aatgggaacc tttatcaata tcccgttcct gctatgaaaa
     1081 atcttcgtca acatcaagca gaatttaaaa cacaacttca aactaacaac acctatgaat
     1141 tagaattact agttcctaga aacgatcttt cttcatttgt tctctttgct aatccgaaag
     1201 gacaaggact atccattact attgatactg caaaggggaa agttatcgtt gaccgctcac
     1261 aagccggaca gcagtacgcc acagaatttg gtactagtcg ccagtgtgac attccaaaag
     1321 atgctactag tatcaatatt tttatcgata agtccatctt tgaaatcttt ataaataaag
     1381 gagaaaaagt atttacggga cgagtctttc cagatgcaga acaatcagga attcaactaa
     1441 aagaaggaca cgttcatggt aaatattttg aattaaaata ttaatatggt tgcaaaatta
     1501 acagatgttg ctgcacttgc cggtgttagc ccaacgactg tatcacgcgt tattaacaaa
     1561 aaaggctatt tatctcaaaa aacagtcacc aaagtcaatg aagccatgag aacccttggt
     1621 tataaaccta ataatttagc acgtagctta cagggaaagt ctgctaaatt aattggactc
     1681 attttcccca acatccgtaa cattttttac gctgaattaa ttgaacacct tgaaattgag
     1741 ttgtttaagc atggttataa aactatctta tgtaatagtg aaaaggatcc tatcaaagaa
     1801 aaagaatacc tcgaaatgct aggagcaaat caagtagacg gtatcatttc aagtagtcac
     1861 aacctaggaa ttgatgatta cgagaaagtt gaggctccca tagtagcatt cgatagaaat
     1921 ttagctccac acatcccgat catctcatct gataatttct ttggaggaaa aatggctgct
     1981 caaaccctta aaaaacatgg ctgtcaaaag atgattatga ttacagggaa tgataattca
     2041 gattctccta ctggacttag gcggctaggt ttttcctatg aatcaaagga atccaaagtt
     2101 atcacagtca caaatgggct atctaacatg agacgtgaga tggaattaaa atcaattatc
     2161 tcgactcaca aaccagatgg tatttttaca tcagacgact taactgcttt attagtaatc
     2221 aaacttatca gccaactagg gttgtccatc ccagaagata tcaaagtgat tggatatgat
     2281 ggtacaagtt tcattcaaga ttacgtccca catctaacca ctattaaaca acctattcga
     2341 gaaattgcgc aattgatggt tgaaatactc ctagctaaga ttgaggggca aaagacaaat
     2401 aaggattata ttttaccagt cagtctcatc cctggtagta gtgtttagga aatcatattt
     2461 ataaaataat ttttagtaat agttattttt caaagcaaat taaaaatgtg gaagaatctt
     2521 cttccacatt tttaattttc atttgttata aattggctta ataaaccatt gacaaatttt
     2581 gctgacgtct catctgaata ttttttagca atctcgataa tctcatttag agctacacga
     2641 tcaggcgttt catcaaaata tttaatttca tagaggccta agcgtaagag agatttatca
     2701 accaatgtta gacgttctaa gctccatcct gattttaagt gactagaaat caaagtatct
     2761 aattcatcct tgtgatctac aacaccatta acaagattaa gtaaaaagat tggaacctct
     2821 aaaactttgt cttcagatac tgttttatca taaccataag caaagtgagc cgcatcaata
     2881 aactctccac cagtttctaa gctaaatagt gtttgaaaag cacgttcacg taaatcacgt
     2941 cttgaatctt taaatacact agtcatctaa gaaatcctca tcaaacaatg acttcaagtc
     3001 tggttttggt gttttctctg gaacaatacc ttcaacatgg atattgaccg aagaaatttt
     3061 tacttctgcc atgtcatata cagctgtttt gacagcttgt tgaatagcaa ttgaaactgc
     3121 tggtacattt acaccatact gcaagtaaac ataaatatct gcagctactg tatcatcttc
     3181 ttcattttta aggtagacac ctctacctag agatttctta ctaaggctat ccgttaccgc
     3241 tttgttacgt aacgaatgaa caccatcaac tttagttgca gcaatacctg taattacctc
     3301 aagtacacgt ggtgaaatga caatttctcc aagattttca gttgtcatag cttatccttt
     3361 cttttagtgt ttactaaggt ttttccttag tagagtaaat tcatcatgaa tctacttaac
     3421 acttttttga gagtcattag gcgcgtgaaa cgtaagttcc ttctgctgta ttgattacta
     3481 acttttgtcc agcttcaata aagtctggaa cgttaacaac aagtcctgtt tccatagttg
     3541 caggttttcc tgaaccagtt acagtagcac ctttgattga tggttgagtt tcagcaactg
     3601 tcaattcaac agttgttgga atttgaacac caataacttc tgttccatag aattgaattt
     3661 ttacttctga gttttcaaga atgtagagta attcattttc aatgttaaca gttggaattt
     3721 catattgatc gtaagtttca ttattcatga agtaagctgt atcatccatc ttataaaggt
     3781 attgagcagg tacagtttca ataatagctt gctcaaattt ttcttcagga cggtaacttg
     3841 tatcaaaagt tgaaccagta cgaacatcac gcaatttcat acgcatgatt gtgttacctt
     3901 tacctggttt gtggtggcta gcttcaagaa cacggattaa tttcccatca gccgtttcaa
     3961 aagtcatgcc tgctttaagc ttacttgctt caatcattta ttaatacctc tttgtaaaaa
     4021 ttatttatca actaatttta tcacaaataa cctgctttct caaatagttt attgatttgg
     4081 tatgtgtctg atttcttgat aaataaataa aatcaccaag agggcgtaaa taagactgaa
     4141 gcacttgaca ctttcctctt acttcagttt tgataacagt tttaatatta taccgatatc
     4201 ttgactaaga ttgaaatact gtttaggtgt ttttagtgac tttttagtaa attttaataa
     4261 aggtagtaaa ctatagttgt cttattgaaa cagataaaat aaatcaccta cggagctatt
     4321 cgtatgaaaa tgtagttatt ctcgatataa gttttagata cgaacttaat ttgctctatt
     4381 gaagctggta aaggaaatcg gggtggtgtt agtggacgtg cgctaagcat tggtactgag
     4441 cttggtgata ccaatcttgc ttactacatc aggtagctct ctgttttagt gccatatgca
     4501 attggaataa gagctgtcat tggtattgaa ggagccctag tccactgatc attgaattac
     4561 taatttagga ggtggaatga tgaattttaa tatgtctcat tatagaaagt tacaactatg
     4621 tcgttttcta atcgtacaga tacctgcgct ggctttatta gctattcttt tatacgggat
     4681 tttcctaaag tacagtctat tttcaaaagt attttttctt atcttttagg ggttgaaata
     4741 gttatcaatt ttgggaagta cataccagtt tacaccaacc acaatttgtc gagtaaagaa
     4801 ttgtaattta gtaaagcctt tttcgttttc ttctgataag tgtatcatga catctatcaa
     4861 tagcatattc acactagtag catattgatt tgtttttgag tagaatttac tttttagatt
     4921 atttctattc tacactagtc tttccctttg gaagattctt tccctctgta tttttccatt
     4981 tttcaatgag acgagaaact ctttcttcct cctcatcatg gtaaccttca aaaacaacct
     5041 catcaatatc ttcttgatta aaatagtaaa cttgttcaag atttaaaccc tcaggaaaaa
     5101 ttccacctag gtaatcaaaa taaacttctt gtccttcttg ctcaacaatt gcacctctat
     5161 ttacaataac taatttttga ttgccatcaa taagatagac aacactccct actggtaata
     5221 acatacttca gttctcccca atataataat ttttcccatt taacgatggt tgaaactcaa
     5281 tgaactttat aaaaaataaa ctgtttgaaa aaataaatcc aaataaaaat aagtttgaaa
     5341 agttttcgtc ccacaatgcc attccccata gaacgatata agaaattatg attgataata
     5401 ttactataa
//
LOCUS       DATRNX010000117         5341 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRNX010000117 DATRNX010000000
VERSION     DATRNX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            114..1040
                     /locus_tag="VBC81_001749"
     CDS             114..1040
                     /locus_tag="VBC81_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6912765.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            1170..2456
                     /locus_tag="VBC81_001750"
     CDS             1170..2456
                     /locus_tag="VBC81_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6912766.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            2583..2795
                     /locus_tag="VBC81_001751"
     CDS             2583..2795
                     /locus_tag="VBC81_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO6912767.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            2919..3716
                     /locus_tag="VBC81_001752"
     CDS             2919..3716
                     /locus_tag="VBC81_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912768.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
     gene            complement(3818..5158)
                     /locus_tag="VBC81_001753"
     CDS             complement(3818..5158)
                     /locus_tag="VBC81_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6912769.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
BASE COUNT         1809 a          795 c         1033 g         1704 t
ORIGIN      
        1 tatctgatag tgcttaacct ctaaagtgat ataattttcg aaaagaatat tttttattta
       61 ttcttgtcaa gaaatatgct taaaaatgct gaaagtttgt tataatacta attatggaaa
      121 aaataatttt gacagcgaca gctgaatcta ttgaacaagt aaagcaattg ctagcaatag
      181 gaattgaccg catctacgta ggggaagaaa attacggtct tcgtttacca cattcatttt
      241 cagacgatga attgcgtgaa atcgctaaat tggtacatga tgctggtaaa gaattgacag
      301 tagcttgtaa tgcactcatg catcaagaaa tgatggataa tatcaaacca tttttggagt
      361 tgatgaaaga aattaatgtg gattatttgg tagttggtga tgcaggagta ttttatatca
      421 acaagcgtga tggttataac ttcaaactta tctacgacac ttctgtattc gttacatcaa
      481 gtcgtcaagt taatttttgg ggacaacatg gagcagtaga aacggtttta gcacgtgaaa
      541 taccttctga agaacttttt aagatgtctg agaaccttga atttcctgct gagattttag
      601 tttatggggc ttctgtaatt catcactcta agcgtccgtt attgcaaaac tattataatt
      661 ttactcatat cactgatgaa aaaacacgtg agcgtggtct ttttttagca gaaccaggtg
      721 atccagaaag tcactattct atttacgaag ataagcatgg gactcatatt tttattaaca
      781 atgatatcaa tatgatgact aaagtgactg aactagttga acatcacttt actcactgga
      841 aattagatgg tatttattgt ccaggagata attttgttgc tattgctgaa atttttgtcg
      901 aaacagctcg tttgattgaa aatggaacat tcactcagga tcaggctttc ttgtttgatg
      961 aacgaattag aaaactgcac cctaaaggac gtggtttaga tactggattt tatgattttg
     1021 acccatcaac agtaaaataa gatttatttg ctacgtgaaa gttttttatc tcaaagcgta
     1081 gccaattgca ctaagaactg catatcgatt ggtatgttaa ttaagataga aaataattct
     1141 tttatcttga aaatagaagg aaatataaga tgtctaatgt aaaaaaacgc cctgaggttt
     1201 tatcacctgc aggaacacta gaaaaattaa aagttgctat tgattatgga gcagatgctg
     1261 tatttgttgg aggtcaagcg tatggtcttc gaagtagagc tggtaacttt tctatggaag
     1321 agttacaaga gggaataaac tatgctcatg cgagagatgc taaagtttat gtagcggcta
     1381 atatggttac tcatgaaggt aatgagcttg gggcaggtcc gtggtttcgt gaattacgtg
     1441 atatgggact agatgcagtc attgtttcag atcctgctct tattgttatt tgtgctacag
     1501 aagcaccagg tttagaaatt catttgtcaa ctcaagcctc ttccacgaac tatgaaactt
     1561 ttgaattttg gaaagagatg gggcttactc gtgtcgtatt agcacgtgag gttactatgg
     1621 cagagttggc tgaaatcagg aagaggacag atgttgagat agaagcattt gttcatggcg
     1681 cgatgtgtat ttcatactca ggacgatgtg ttctatcaaa ccatatgagc catcgtgatg
     1741 ctaatcgtgg cggttgctct cagtcatgtc gttggaaata tgacctctac gatatgccat
     1801 ttggacaaga acgtcaatcg ttaaaaggcg agattccaga acctttctca atgtcagctg
     1861 tggatatgtg tatgattgag catattccag atatgattga aaatggcgta gatagtttaa
     1921 aaatagaagg acgtatgaaa tccattcatt atgtttctac agtaactaat tgctataaag
     1981 ctgctgtaga tgcctatatg gaaagtccag aagcttttga agctattaaa gaagacttga
     2041 ttgatgaact ttggaaggtt gcacaacgcg aattagcaac aggtttttac taccatacac
     2101 caactgaaaa cgaacaactc tttggagctc gtcgtaaaat tcctcaatac aaatttgttg
     2161 gggaagtggt ttcatttgac aatgctaaaa tggaggctac aattcgtcag cgtaatgtta
     2221 ttatggaagg agatcgcgta gaattctatg gtcctggctt ccgtcacttt gaatgtttta
     2281 ttgaaggtct gcgcgatgct gaaggaaata aaatagaccg tgctccaaat ccgatggaat
     2341 tattaaccat aacattacca aatccagtaa aaaaagggga tatgattcgt gcttgtaaag
     2401 aaggattagt gaacctttat caaaatgatg gtactagcaa gactataaga gcttagatac
     2461 ttttataact attttgtcat tttacatggt caatattact aaaaattgtt aaaatagaag
     2521 aagttcattt aaatagaaca aaaagctata aaaataacga atagtataag tggaggtata
     2581 aaatgtcaga atttacattt gaaattgttg agaagttatt ggtcctatct gaaaatgaaa
     2641 aaggttggac aaaagagttg aatcgtgtta gtttcaacgg agcgccagct aaatttgatt
     2701 tgcgtacatg gagtccagat catacaaaaa tgggtaaagg tatcacttta tctaatgaag
     2761 aatttaaagt cattttagat gcttttcgta aataaataaa aagtggtttc aaaaaccact
     2821 ttttttgtcg agacaagcga gtattcagta aacatgttga ttaaaaaaat agaattttta
     2881 aactaaaaga attataatgg aggtaaaagg agtcctctat gattagacat tcatttaaaa
     2941 gtaagatgtt attgttagtc tttatcttaa caataatagt gaattattta tccgcaacag
     3001 gtttgttaac agggaacagt caaaagagtc tgtctgatcg ttatcaaacg ctcttgacac
     3061 cagcaccatt agccttttca atttggtcag tcatctattt attgaccttt ttagtaatat
     3121 taagagctat attcagtaag agtcaatctt accaagataa ctttgctagc atttttccat
     3181 actttttggg tcttctatta gttaataata tatggactgt attttttacg agcaatttga
     3241 ttggcctatc aacaataata atttttgctt actgtattct tctggttatc attatcaaga
     3301 tactaagtaa aaataaatca aaattactgc ttagaataac atttggaatt catgcaggat
     3361 ggttgctagt tgcgtcattg gtcaatttag cggtctactt agttaaaatt gattttaatt
     3421 acccattacc aaaggtatat atagctatca ttgccttgat ttttataact gttttaagtc
     3481 tttacctggc tagggtatta caaaatgctt acttaatctt atccgttttt tgggcttggt
     3541 tgatggtttt taaagcacat ttagaaggaa attttcaaca cctatatttt agtttccaaa
     3601 ttttatcttt gattgcagca attatccttg gcgtatcgtt tttcatcatc ggttattata
     3661 atgtcaagga gaggtacaag caaaaacgta ctagtcctta ctcacgggtt ctttaaaaga
     3721 aatacaaaaa gctccatcat tatgtacttt cctctatcat tagatgtatg tctcatgata
     3781 gaggttcagt tcattagtgg ggcttttttg tattgtatta aagtaaatcc atgattaatt
     3841 tcaaattgag taatgttaaa atgatagcga taaggtatgc taaataggta ttccatttag
     3901 tattagcatg ctctcccata attgattttt gcgatgtgaa ataaatcaat ggaaagattg
     3961 aaaatggtaa agctaaggaa agaaaaactt gtgaatagac gattagttga tctaacgtat
     4021 tttcttcgcc tccaactagt agggcgatag caaaaattgg aagaagagtt aataatctag
     4081 tacaaagcct tattaaccat tgaggtaatt tgaagtgaag gaaaccttcc ataacgattt
     4141 gtcctgttaa agtacctgta attgttgaat tttgaccact tgctagtagg gcaactgcaa
     4201 aaagggtaga aaggaaagaa ctggctactg caccagtaat agttttatca gataaggcaa
     4261 gatacatttg agaaaaggca ctgatatcat tagcatgccc ataaaataga ctagctccta
     4321 aaactaatag tagcgaattc acgacaaatg caagtgatag ctgaatgtta gaatctaagg
     4381 tcatgaaacg tacagctttt tttattgaag atttattatg ataatccacg cgtctagtct
     4441 gagataaaga agaatgtaaa taaagattat gtggcatgac tgttgcgccg ataatgccaa
     4501 gagcgagggt taaaggacta tttttccctt catgtgatat gttaaaaagc tcatggtgtg
     4561 gtaaaaatcc tttgaacata gcatctaaat caggttgtga taaaacaact aaatatgtaa
     4621 agattatcaa aattgtcaat atgagaacag aaacaaaggc ttcaattttt tggacgccta
     4681 acttcattaa gagtaaaagt aagaatacat ccaaaatagt aatcatgata gacagtaaca
     4741 ggggccagcc aaataataag tgaagagcaa tggcagagcc aatgacttct gctaaatcag
     4801 tagcgataag ggctaactca atgataataa atagcaggta acgaagcggt tttgggagtc
     4861 gagaagctgt tgcttgtgct aagtcctgtc gtgtaacaat accaagcttg ccagccattt
     4921 gttgtaattg catcgccatc aaagaagata ctaaaacaac aaacaatagt aagtaacgat
     4981 aggtagctcc accaataacg ctagtaatcc agttacctgg atccatataa ccaactgcaa
     5041 ccaaagctcc aggtccaaga aaagctctta aagtgttcca aaaagagcta ttgtgaggaa
     5101 cctcaacgct ctgattaact tcggataagg atcttttgac ggaatgattt tgattcatgt
     5161 aatactatac tttctagtaa tctcaagcca ttataacaaa tagaaaatag aaaaacaatt
     5221 aaaaattaat gctacttaat aatcgattga ggtgctgttt agtattagag aactattttc
     5281 ttaacagtta catttgaaat aacaaaataa cagatttcta tcgattattt ttagttccgt
     5341 a
//
LOCUS       DATRNX010000118         5288 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRNX010000118 DATRNX010000000
VERSION     DATRNX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            32..1198
                     /locus_tag="VBC81_001754"
     CDS             32..1198
                     /locus_tag="VBC81_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6912770.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            1304..2173
                     /locus_tag="VBC81_001755"
     CDS             1304..2173
                     /locus_tag="VBC81_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6912771.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            2176..3129
                     /locus_tag="VBC81_001756"
     CDS             2176..3129
                     /locus_tag="VBC81_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6912772.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            3130..3894
                     /locus_tag="VBC81_001757"
     CDS             3130..3894
                     /locus_tag="VBC81_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912773.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            3894..4604
                     /locus_tag="VBC81_001758"
     CDS             3894..4604
                     /locus_tag="VBC81_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912774.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            4623..5282
                     /locus_tag="VBC81_001759"
     CDS             4623..5282
                     /locus_tag="VBC81_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6912775.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         1639 a          787 c         1156 g         1706 t
ORIGIN      
        1 ttagttacca ttttcaaaaa aggagaaaaa tatggaaaag agacttagtt taggagcgct
       61 tgttttagct agtacagttc ttttagcagc atgtggaaat gtcggaggag gagcatcttc
      121 gacaggaaca aaaataggga aagatattaa ggtagggtat aactgggaat tatcaggaaa
      181 cgtttcttca tatggaaatt caatgaagaa tggagctgac ttagcagtaa aagaaattaa
      241 tgctgctgga ggggttggcg gcaagaagtt aaaagtccta tcacaggata ataaatcaga
      301 aaatgcagag gcagcaacgg ttgccacaaa cttggttacc aaaggagcta atgtcattat
      361 cggaccagca acatcgggtg cagctgcatc ttcaactcca aaagtaaatg cagcagcagt
      421 tccaatgatt gcacctgctg cgacacaaga caatttagtc tatggttctg atggaaaaac
      481 cttaaatcag tatttcttcc gagctacttt tgtcgataat tatcaaggaa agctattgtc
      541 tcagtatgct acagacaacc ttaaagctaa aaaagttgtt ctattttatg ataattcatc
      601 agattactca aagggggtag caaaatcatt taaggaaagt tatagtggaa aaattgttga
      661 tagtatgaca ttctccgctg gtgatactga tttccaagcg tcattgacta agttgaaagg
      721 gaaagaatat gatgctattg tgatgccagg ttactatacc gagacaggat taatagttaa
      781 gcaagcgcgt gatttaggta tctctaaacc ggttcttggg cctgatggtt ttgatagtcc
      841 gaaatttgtg caatcggcaa cacctgtagg agcttcaaac gtttattatt tgacaggttt
      901 cactacacaa ggatcaacca aagctaaagc tttccatgat cattacgtta aggcatatgg
      961 tgaagaacca tccatgttct cggctttatc atacgacgct gtgtatatgg cagctaaatc
     1021 tgctaaagga gctaaaactt caattgatat taaaaaagct ttagctaagc tgaaagattt
     1081 taaaggtgtg acagggaaaa tgtctattga taaaaatcac aatgttgtta aatcagctta
     1141 cgttgtcaaa ttagaggatg gaaaaacaag tagcgttaac attatttcag caaaataagt
     1201 attctctaga aaaatgggag tttaattttc agctcccctt ttttaaaaag taatttttca
     1261 gaataattcg acaataatat ataatagaaa gtatggttat aatatgcttc aacagctcgt
     1321 taatggactt atcttaggat ccatatatgc tttacttgcc ttaggatata ctatggtata
     1381 tggcattatt aagctgatta attttgctca tggggacatc tatatgatgg gcgctttcat
     1441 gggctattat ttgattaatc atcttcatct taattttttc ctagctttat taattgcaat
     1501 gctggggtct gctttcctag gtgttgtaat tgaatattta gcttatcgtc cactgagaaa
     1561 atcaactaga attgccgcgt taattacagc tattggtgtt tcttttcttt tggagtacgg
     1621 gatggtatac cttgttggag cggatactag ggcatttccg caagccattc atactgttaa
     1681 atataattta ggaccgataa ctataaccaa tgttcagctc atcattttag gtattgcgct
     1741 attactaatg cttactttac agttcatcgt tcaaaaaact aaaatgggga aagctatgag
     1801 agctttatca gtggatagcg atgccgcaca attgatggga attaatgtta accgtaccat
     1861 tagttttaca tttgcgctgg ggtctgcttt agctggagca ggtggagtac tgattgggct
     1921 ttattataat tctgttcaac ctcttatggg ggtaactcca gggttgaaag catttgttgc
     1981 agctgtctta ggtggtattg gtatcattcc gggagcggct atcggtggtt ttgttattgg
     2041 cattttagaa actttagcga ctgcacttgg tgtgtcagat tttcgagatg gtatcgttta
     2101 tgctatttta attctcattt tcttgatccg tccagcagga attcttggaa aaaatattaa
     2161 ggagaaggtc taggcatgaa agaaaatatg aaatcaattt tatcttggtt agctatagta
     2221 gtgttaggtt atctcttgat tagcgtgcta atatctatgg gtatatttaa cctctatcat
     2281 attcaaatta ttgaaacaat tgggattaat gttattttag cagttggttt aaacctaatt
     2341 gtaggttgtt cgggacagtt ttcactagga catgcaggtt tcatggctat tggcgcttat
     2401 gcagttgcta ttatcggcgt taaaatgcca acttatgtag gttttttgat agctatactt
     2461 gttggaactc ttgttgcagg aggtattgcc ttaggtgtag gaattcctac tttgagacta
     2521 aaaggtgatt atttagctat agctacttta ggtgttgctg agattatccg tattttgttg
     2581 gttaatggag gtgatattac gaatggtgct gcgggtatca tggggattcc gccttttaca
     2641 acctggtctt tagtttatgg ggtggcagtt gtgagcctta tccttgcaat gaattttttg
     2701 cgtagtccct tagggcgtaa tacaattgct atacgagaag atgaaattgc agcagagtct
     2761 atgggtgtgg atacaacaaa agtgaaagta attgtttttg tttttggagc tattttagct
     2821 tctatcgctg gtagtttaca ggctggctac gttggaactg ttatgccaaa agacttttca
     2881 tttatgatgt ctgtcaatgt tttaattatt gttgttttag gtggtttagg ttcaatgaca
     2941 gggacagttt tagcagcaat tttattagga ttattgaata tgttactaca agactacgct
     3001 tctgttcgaa tgattatcta tgctttagcc ctgattttaa ttatgatttt tagacctagc
     3061 ggtttattag gaacgaaaga gttaactcta tctcatctat ttcgtaagac gagtaaggga
     3121 ggcaattaga tggcacttct tgaagtaaaa aatttaagta aacatttcgg tggcctaact
     3181 gctgtaggtg atgtttcgat gaaattacat aaaggtgaat taattggctt aatcggcccc
     3241 aacggtgcgg gtaagacaac gctttttaat ctcttaacag gagtatacct tcctagtaaa
     3301 gggacaatat cacttgatgg taagatatta aatgggagga aaccagctaa aatagcttct
     3361 ttaggtttag gtagaacttt tcaaaatatc cgacttttta aaaatatgac tgttttagac
     3421 aatgtactag taggactcag taatcatcac ttatcacatc ctattgctag ttttttacga
     3481 ttacccaaat actatcatag tgaaaaagcc ttaaggaaaa aggcactgga gctattagag
     3541 atttttggtt tgaaagcata tcaagatgct cttgctaaga acctccctta tggaaaacaa
     3601 cggcgtttag aaattgttcg tgctttagca acagaaccaa aaatattgtt tttggatgag
     3661 cccgcggcag gaatgaatcc tcaagaaact gcagaattga cgcagttaat tagtcaaata
     3721 aagagtgatt ttgatattac tattatgctg attgaacatg atatgaactt ggttatgcaa
     3781 gtaacagaga gaatttatgt attggaatat ggtcgtctga ttgctcacgg gacacctgaa
     3841 gaaattaaaa ataataaacg tgttatagaa gcatacttgg ggggggagct ataatgacca
     3901 tgttgaaagt agaaaatcta tctattcact atggtgttat tcaagcggtg aatgatgtct
     3961 cgtttgaagt taaccaaggt gaggttgtaa ccttaattgg agccaatggt gcaggaaaga
     4021 catcaatttt gaggacaatt tctggtttag taagaccaag tcagggttct atttctttta
     4081 tggggaaacc tatacataag ttagcagcta gaaaaattgt tggcaatggt ttagctcagg
     4141 ttccagaagg gcgccacgtt ttctcaagtt tgtctgttat ggaaaattta gaaatgggag
     4201 cctttcttca aaaggatcgt gaacaaaatc aaaaaatgtt aaaaaaagtg tttgatcgtt
     4261 ttcctcgctt agaagaacgt aaaaaccaag atgcagcaac cttatcaggt ggtgagcagc
     4321 aaatgctcgc tatggggcgt gcattgatga gtcgtcctaa attattactt ttagatgaac
     4381 agtcaatggg cctagctcca atttttattc aagaaatttt taatatcatt gaagacatca
     4441 aaaaacaagg gactacagtt cttttagttg aacaaaatgc aaataaagct ctgacgattg
     4501 ctgataaagc atatgttcta gagactggga aggttgtttt atcagggaca ggtaaggaat
     4561 tactagtttc agatcaagtt cgtaaagctt acttaggtgg ataatattta gaggagtcag
     4621 gtatgccagt gaaagatttt atgacaaaaa aattagttta tgtctctcca gatactacag
     4681 ttgcagaagc tgctgattta ttgagagaac accacttaag gcgtttgcca gttgttgaaa
     4741 atgatcagct agtggggtta gtgacagaag ggacaatggc agaagcacaa ccttctaaag
     4801 caaccagtct ttctatatat gagatgaact atcttcttaa taagacaaaa attcgagata
     4861 ttatgattaa agatattgtg actgtttcac agtatgctag ccttgaggac gctatttatt
     4921 tgatgatgtc tcgaaagatc ggtgttttac cagtagttga taatggacaa ctttatggga
     4981 ttgtcacgga tagggatgtt tttaaagcct tccttgaaat tgcggggtat ggtcaagaag
     5041 gttatcgctt ggttatatta gctgatgagg ggataggggt actatcaaaa gtcctaaatc
     5101 gtctttctag tgcaaattta agtgttaaac gtttagtcat tattgaacga aaagcaggta
     5161 agaaagcggt cgagatacaa ctagaaggat acgctgacaa ggatgtttta aaacaagaac
     5221 tagtatttga tgatgttatt gtggaaactt tagaaaagac catgaaaaaa ccactttctt
     5281 aaaaatat
//
LOCUS       DATRNX010000119         5187 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRNX010000119 DATRNX010000000
VERSION     DATRNX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            160..579
                     /locus_tag="VBC81_001760"
     CDS             160..579
                     /locus_tag="VBC81_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6912776.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            576..872
                     /locus_tag="VBC81_001761"
     CDS             576..872
                     /locus_tag="VBC81_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912777.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(936..2243)
                     /locus_tag="VBC81_001762"
     CDS             complement(936..2243)
                     /locus_tag="VBC81_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6912778.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(2252..2812)
                     /locus_tag="VBC81_001763"
     CDS             complement(2252..2812)
                     /locus_tag="VBC81_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912779.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2766..3275)
                     /gene="tsaE"
                     /locus_tag="VBC81_001764"
     CDS             complement(2766..3275)
                     /gene="tsaE"
                     /locus_tag="VBC81_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6912780.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3358..4779)
                     /locus_tag="VBC81_001765"
     CDS             complement(3358..4779)
                     /locus_tag="VBC81_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6912781.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4870..>5187)
                     /locus_tag="VBC81_001766"
     CDS             complement(4870..>5187)
                     /locus_tag="VBC81_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6912782.1"
                     /translation="VSSILFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFG
                     IHIGRYARLNSWDILTKPQVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKK
                     LEK"
BASE COUNT         1759 a          993 c          781 g         1654 t
ORIGIN      
        1 tttaggtcat gctaagataa gtatgatggt aaatatagat atcacaatca atacaggcta
       61 cttttatcca cctgtacgac ttatgaccta tcaaacgata aaaatttacc attccttaat
      121 agctacccaa taaatttttc aacatgaaag gaattagtta tggataattg tattttttgt
      181 aaaatcatca gtggagaaat ctcttcatca aaagtttatg aagatgatga agtattagct
      241 tttctcgaca ttacacaaac tacgactggg cacacccttc ttattcctaa aaaacatgtt
      301 cgaaatgtgt tagagatgga tgaaaaaact gcacagataa cctttgaacg cttgcctaaa
      361 gtggcgcgtg ctgtccaagc tgctacaaaa gccaaaggaa tgaatattat taacaacaat
      421 gaggaaattg ctggacaaac tgtttttcat gcacacgtcc accttgttcc ccgctttgat
      481 gaatcagacg gtatcaaaat tcactacact actcatgaac ccgactttga ggcacttgca
      541 aaattagcca aagaaattag aaaagagatt caattatgaa aataggtcgt ttaattgcac
      601 taggtttagt tagtttagga gcgcttgaac tttacaaaaa tagaaaaact atcaaggatt
      661 cctatcaaaa tacaaaaaat gagacagatt cagctaaatt aaaattagaa cgtatcaaaa
      721 atgatcttgc tattattagt caggaaaaag aaaaaatcag attaatttct caagaactca
      781 atcataaatt ccaagtgttt aataaagata ttcaacctcg tttagaagaa attaatcaac
      841 gcatggctaa atatcaagag aaagacgaat aagccgacgg tataaaaata tctactctaa
      901 atataaaaag agcaaataat gctctttttt atattttatg gattagtttg acttggtact
      961 ggttgcgttc ctgtgtttgg attaggcgtt gcagccccgt tataattatt aacacttcca
     1021 cttgaatcag cctgacctgt gttagtgtta ctgctatagt tactagcagg agtgctacta
     1081 ttatagtaac tttgatgagt tgtttgatta gtctcagaac tataagtagt attaccactg
     1141 taacttggtg gtgcttctga ataaggtgtt gtattataat tattctcttg tgttgatgaa
     1201 taagtagaag aatcattact agcagtagta ccatagtaat cttcatatag aatcgcgctt
     1261 gtcttcagag ttttactacg ctttttatcc agctctttct taattctatt ttgaactgca
     1321 agtagatgtt tcttagttaa aatttgataa gagccaccat ctgatagagt agcgtcttca
     1381 cccttcaact gataagattt aatatgttcc aatgaatctt tataagctaa caaattagga
     1441 atcgtttttg atgatatctc aatattagtt tgcatgttat tacttactgc ggaaagaatt
     1501 tttttgtatg aactaatact atttaacgcc aatatttttt taaggacttt ttgaattact
     1561 tcacgttgtc ttttttgacg cccataatct ccctctggat catcatagcg catacgagaa
     1621 taaacaagtg cttgttctcc atttatttta tgtgtccctg gttcaacaac agccttgtac
     1681 tctggttcat tggcagcaat tgatattgga aagtcaaatt tattagttac tgttatacca
     1741 ccaacagcat tgaccaaatc aactaatcct tgcatattaa tttgcataaa gtaatcaaca
     1801 ttaatatcta ataagtcttg aacagtcatc aatgccattt ccgcaccacc agaagcatag
     1861 gctgcattta gctttgcttc tacgccagtc tgtccattat ttttgggacc actcaattta
     1921 atcaatacgt cacgttctaa gcttgtcatc gttgttttat tagttttagg atttatagtg
     1981 actaagatca tagaatcgct atttcctgac cacttagatt ttcgatgctc tgaacctgtg
     2041 tccacaccca ttaatagtat tgaaaacggc tttgtttctt caatagcatg actttttgat
     2101 ttagctagct taaaatcttt aaaagtctta gacaattcat tagtcgaaaa attataagct
     2161 gaggcaacat agactcccaa tgcaattact gttgttaaaa taattgcaga aaacattaag
     2221 gttatttttt tccaaatttt catgtatatt cctaatctat caacttactc attctgtaaa
     2281 tgtctagaaa ttctccattt tctcttttaa tacctcttgt ttgaagccca tctatttcaa
     2341 aactgaattt ttggtagagg tgaatagcac gttcattacg tccttggact gatagttcta
     2401 agcggcgtgt aatatcgtta tctgacgccc attctattgc ttcttccatg agaatatgac
     2461 caataccata cccccaataa tccttctgaa cagcaataaa aagatcccca atgtgttcaa
     2521 tttcaatatc agactgacta atgattgtta tcaagccgat cactttattt tttaatttag
     2581 caatcaaaca aatgcaattt ttcgttatta aggtattttc aataaacgtt tccatttctg
     2641 gcaaagatag ttgattgctc accgtatgca aaataaaatc tgtttcctca gtaacagtat
     2701 ttaaaaattc aagaagcgca gatgcttcct ctctcgaagc ttctataaaa tcaacagcaa
     2761 attgtttaga catcttgaat cgcctcaata atttctcttg cgcgatgccc ataagcttct
     2821 aactgaactt gacgtccttg attgctcctc gtaataacaa tttctaagta gttattgata
     2881 aggttatcac taagcaactc tccccattca ataactgtta ctccttgacc aaacaagaag
     2941 tcatctaagt caatagaatc aggatcatct ccaatacgat aaacatccaa atggtaaagt
     3001 ggtacacgcc cttcatattc tctaacaata gtgtaagttg ggctcttaat catttgtttt
     3061 atgtctaatc cctttgcaat accttttgta agagttgttt tgcctgcacc taaattacca
     3121 gtcaataata cgatatctcc tgactttaaa acagtaccca gtttttgccc tagggcaata
     3181 agctcttctt cattttgagt ataaaacata gagatattat accagaaata tctctgtctt
     3241 ccaacagttt gctgattctg acagccccat agcatatatt tcttcacttt tcttaagaaa
     3301 aaaggaaacc aggctacaaa tctgatttcc ttttatctta tttatcaaac tataatatta
     3361 caaaatagcc aagctgataa agtttagaat aaataatata tctaaaatcc acagtaccac
     3421 atgaatatct tttgcttgtc ctttgatgac tttagctagt gtataggtca agaaaccagc
     3481 tgcaatacca taagtaatgc tataagtaaa ccccataaaa agagaagtaa agaaagctgg
     3541 gatagcttct gacatatcat cccatttgat atcttttaaa tttgaaagca tcataatacc
     3601 aacaataacc aatattggtg cagtagcttg actcggaacg atagatacta gtggactaaa
     3661 aaaactagat atagcaaaca aaccagcaac tactaaggct gtcaatcctg tacgaccacc
     3721 agctccgatt cctgctgctg actcaacata ggttgtaacg ttagatgttc cacaaatagc
     3781 accaaaagtt gtcccaatta agtctgaata gagtgctttg tctaaactct tagattcatg
     3841 attatcacca gtcgttgcta agataccaac tttttctccg gtaccaatga gcgttccaat
     3901 ggtatcaaaa atatcagtca gtgaaaaagc aagaatggcc attaatacac ttggtagacg
     3961 tgaaggatta gaaaataaag aaattaatcc tttctctcct aatgcaactc caaatacttg
     4021 cttcaaatct ctaaaagagg ccgagaggtt agttgcttcc caattaatag catctaactt
     4081 aaccactccc attaaaatac ctaagagggt cgttgttaat atcgatagaa taatccctcc
     4141 acggatacct tttacaataa agaagatggt aatgcttaaa ccaatcaaag ataataaaat
     4201 tgctggatta tcaaacgaca ctagccctgg cgttgctgaa gaatttgctg taatagtagc
     4261 caatcctttc gcagcaccct ttccaacaac atcatacgtc ccaggatcaa ttgaaaattt
     4321 caaaaaacca gcatttttaa tcccaacgta tgctaaaaaa gttccgattc ctgctgtaat
     4381 ggctgatttt aaagttgttg ggatagactc aataatcatc tttctaactt tcgttaaagt
     4441 aataatcaga gaaatcaaac cacaaataaa taccatggct aaggcttctt gccatgtata
     4501 tcccaatgca aagacaactg tatatgtaaa gaaagcattt aatcccatcc caggagcttg
     4561 tgcataggga aggttagcat agaaagccat aacagatgta gctacaacgg caccaataat
     4621 cgtcgctaag aaaactcctt gtgctggcat cccagtctga gataaaatag ctggatttac
     4681 aaacaaaatg tacgacattg caaagaaagt cgttagacca gcagtgattt cagtccgaat
     4741 tgtcgtcccg tgttccttta gcttaaaaaa cttttccatt ttaaaatcgg attctccttt
     4801 attttttgtt aatatatatt aatcgttcgt atatttacga acgattaata tatattaaca
     4861 aaaaataatt cacttttcaa gctttttccc tgcagaataa tccataaaaa taagacacaa
     4921 tacctgtaaa aatgtaaatc ctaaaataaa gtggaatgaa tccctactcg gaaccgctaa
     4981 aagttcgtta attaccactt gtggcttcgt taagatatcc catgaattta gacgggcata
     5041 acgaccaata tgaataccaa aacttgaaac aaatgataca attccaataa ttccccatct
     5101 taaaaatata ttgctaattc taaatttacg cataatcaca gaaaaacttt cgatacctga
     5161 taaaaaacca aacaagatac tagatac
//
LOCUS       DATRNX010000120         5185 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRNX010000120 DATRNX010000000
VERSION     DATRNX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..328
                     /locus_tag="VBC81_001767"
     CDS             <1..328
                     /locus_tag="VBC81_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6912783.1"
                     /translation="LNAVEAIDKQKKGTISVHTEELEDFIKIEISDNGPGIPDKMKHM
                     IFKPGFSTKFDANGDIYRGIGLSHVRILMEEQYQGTITVCPNQPNGTTFTLLFRKNRF
                     KLGDNT"
     gene            325..1158
                     /locus_tag="VBC81_001768"
     CDS             325..1158
                     /locus_tag="VBC81_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6912784.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            1380..2102
                     /locus_tag="VBC81_001769"
     CDS             1380..2102
                     /locus_tag="VBC81_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912785.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            2105..3619
                     /locus_tag="VBC81_001770"
     CDS             2105..3619
                     /locus_tag="VBC81_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6912786.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(3698..>5185)
                     /locus_tag="VBC81_001771"
     CDS             complement(3698..>5185)
                     /locus_tag="VBC81_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEO6912787.1"
                     /translation="LQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAFPIA
                     IIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGIIIV
                     SLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKILQGK
                     SCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVNNKC
                     LIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVGEDA
                     LVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLEGRP
                     EKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQKIPN
                     GVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHFYSF
                     NGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
BASE COUNT         1827 a          952 c          825 g         1581 t
ORIGIN      
        1 tttaaatgca gtagaagcta ttgataaaca aaaaaagggt acaatcagtg ttcacacaga
       61 agaattagaa gattttatta aaattgagat tagtgataac ggaccaggaa tacctgataa
      121 aatgaaacac atgattttca aacctggatt ttcgactaaa tttgatgcta atggtgatat
      181 ctatcgagga atcgggctat cccacgtgcg tattttaatg gaagagcaat atcaaggaac
      241 gataactgtt tgtcctaatc agccaaatgg aacaacattt acactgttat tccgtaaaaa
      301 tcgatttaag ttaggagata atacgtgaaa ttctatatta ttgatgatga ccccactatc
      361 actatgattt tacaagatat tattgaagaa gatttcaata atacagtagt cagagtgaat
      421 aatgtctcca gtaaagcata caacgaattg ctgattgcag atgttgatat cgtattgatt
      481 gatttattaa tgccaatatt agacggtgta acattagtcc aaaaaatcta taaacagcgt
      541 tccgatttaa aatttattat gatttcccaa gtcaaagata atgatttacg acaagaagca
      601 tataaagcag gaatcgaatt ttttattaat aaacccatta atattattga agtaaaaagc
      661 gtcgtgaaac gtgtcactga tactatcgaa atgcaaaaaa aattaaatac aatccaaaat
      721 ctcctagaaa atacgccatc atatcaaaag cctataacga ctagtaacct cactaaaatc
      781 cgttcaatac tcagttattt aggtattact tcagagacag cctacacaga tattcttaac
      841 atctgtgaac ttttattaaa acaagaatta aattttgccc aatttgattt tcaaaaagaa
      901 ctttctatcg atgaacacca acaaaaaatc attttacaac gtattcgtcg tgccgttaaa
      961 aaagctatga taaatatggc tcatctgtac attgatgatt ttgaaaatga attaacactg
     1021 caatatgcta atgcattgtt tggatttcag aatatccata atgaggcaca acttattcaa
     1081 ggaaaaagca tgtatggtgg taaaatttca cttaaacgtt ttttcgatga acttatttta
     1141 cagagtaaaa cgttttaaga cttgtttaag catggcctta tatactataa ccatcctaaa
     1201 acttttcttt tcataaatct ccaacaaaca ccttgtctac cgcaaggtgt ttgtttttgc
     1261 ttctagttta tcgtttctga caatttaaat gtcagatagt tgtttgaatg tctactttat
     1321 gctaatataa ccctaatatc atacgaccta cactcgttac gcactagaaa gtaaattaca
     1381 tgatttcatt tgaaaatgtt agtaaatcat atggtgatca taccatcatt gataatatta
     1441 gttgccatat tcaaagagga gaattctttg tccttgtggg ggccagtggc agtggtaaaa
     1501 ctaccatcct caagatgatt aatcgtctca tcgaaccaag ccaaggcgct attactttag
     1561 atggggaaaa cattacatca cttgatttgc gacaattacg cttagaaact ggttatgtct
     1621 tgcaacaaat tgcacttttc cctaatttaa cagttggaga aaatatagaa cttatccctg
     1681 aaatgaaggg ctggtcaaaa ggtgaccaaa aaaaagccgc tagcgacctt cttgataagg
     1741 tgggtttacc tgctaaggac tactttaatc gctatcctca tgagctctct ggtggggaac
     1801 aacaacgcat aggtatccta cgagcaatcg ttgctaaacc taaggtacta cttatggatg
     1861 agccattttc tgctttagac cctatctcaa gacgtcaatt acaagatatc accaaacaat
     1921 tacagtctga attgggtatt acattagtgt ttgtcaccca tgacatgaag gaagctatgc
     1981 gtcttgcaga tagaatttgt gttattaagg aaggtaagat agtgcaattg gatagaccag
     2041 aaataattca aaataatccc tctgaccaat ttgttagaac cctatttgaa gaggagaact
     2101 gagtatgaca acactaatca ctacctttca agagcgtttt ggagattgga ctcaaagtct
     2161 tattgaacac ctccagctat cactactaac acttatttta gcaactttaa ttgctattcc
     2221 actcggtatc atcattagtc attataaaaa aatcagtcat gttgtccttc agataactgg
     2281 gatatttcaa acaattcctt ctttagcctt gcttggttta tttatacctt ttatgggaat
     2341 tggaactgta ccagctgtcg ttgcactaat tatttacgct ctatttccaa ttttacaaaa
     2401 tactgtaaca gcactgatgc aaattgatgc taatctcatt gaggcagcaa ccgcttttgg
     2461 aatgacacgt tgggaaaggc ttaaaaagtt tgagttagct ttaagtatgc ctgttatcat
     2521 ctcaggcatt agaactgcct ctgttatgat tattggtaca gctacattag ctagtcttat
     2581 cggagctggt ggccttggat catttatttt gttagggatt gatcgcaaca atccttctct
     2641 aattctcata ggggcaatct cttctgctgt cttagctatt atatttagtg gtttaattgg
     2701 attactagaa aaggctagat tacgaaccat agccgtttct ggtatacttt tactagcagg
     2761 cttaggacta tcctatgctc caaaatggat gcctggaaca aatactgcaa cgataactgt
     2821 tgcagggaag ttaggaactg aaccagatat tttaattaat atgtacaaag aacttattga
     2881 ggatcagaca gatatcaaag ttaagcttaa acctaatttc ggaaaaacaa ctttcttata
     2941 tcaagcccta aaatctggtg atattgatct ttacccagag tttacaggta ctattacatc
     3001 aagtttactt aaaaatcctc ctaaagtaag taataaccct aaacaggttt acaacttagc
     3061 caaaaacggt attttaaaac aagataagtt gtcacttttg tcacctatgg cttatcaaaa
     3121 cacctacgct gtagctgtta aaaaagatta cgctgaagct aatcaactca aaaatatcag
     3181 cgacctaaaa aaattagata aactaaaagc tggtttcact ttagaattta aggatagaga
     3241 ggatgggagt attggtttac agaaacatta tggtttaaac cttgatatct caaccttaga
     3301 acctgcttta cgataccaag ctatcaatag taaagatgtc aatattattg acgcttactc
     3361 taccgatagt gaattaatcc agtatcaact tcaaattctc aaagacgata agcatctatt
     3421 tccgccatat caaggagcac cgctactaag gcaagatacg attaaaaaat accctcaagt
     3481 aaaaaaagct ctcaataaac ttgctggtca tatcactgaa aaagaaatgc aagaaatgaa
     3541 ctaccaagta gctgtaaagc ataaatcagc agctacagta gctaaacaat atttaaaagc
     3601 acatcatatt ataaagtaga ttcaagcaat caatggtatt aaatgattta aaaagagcat
     3661 gtcgcatgac atgctctttt taattgccaa ataaagctta tttaacaatc ttaattttac
     3721 tatcttccat taatagggca caaatagcac aaattaacca ggacgaacga gaacaagaaa
     3781 tataacgttc cgaccacaat ggtgtaaact tcttcttata cttgtgtaaa ccattaaatg
     3841 agtagaaatg actaccgaaa tgatagacaa gatacgccat tctctcttta gcaaaacttg
     3901 tttcaacgcg accaactcct gataaaggtg ccatccccaa atcaaaatag tggtatccct
     3961 tctcttgata ataagagaat aatgataaaa agaggaaatc cataacgcca ttcggaattt
     4021 tctgtttatc gtgacgcatt aaatcaatag agataatact cttttcatag tttggcataa
     4081 tattagcaaa agcaacaact tcgtgttcag cattttttac caaagctatt ggggcttgtt
     4141 ggaaataatc tttattaaaa tatcctagtg agaaaccttt ttcaggacgt ccttctaacc
     4201 aagtattaga aatctcttcc aaactattta gtagctcttg actatgtggc gattgtacaa
     4261 cttcgaaata gaaaccatcc ttttcaactc tatttagggc atttctgaaa ggtttgtact
     4321 tattcccttt aagagtaaac gtttctaaat taactaaagc atcctcacca actttcataa
     4381 agtcaaaacc atactcatgt aaaagtagtg tcaacttctg tccaatactg taaaaaacaa
     4441 ggtcatagtc tagcttatca gcatcatcaa taaacgattc aatagcttca cgaatataag
     4501 tgtcatcacc ggctggttcc cccataataa gacatttatt attgacaatt acaaattgga
     4561 acgcaacgca atcttctcca tttttttggt accagtagag ccttttatca tttaaaaagg
     4621 ctaaaccgct atccgaagaa ccaccgtaag cttgaagtag ttttttataa cgatccacat
     4681 tgaacacatc accaatctga caagattttc cttgtaaaat cttaaccaaa cataaataaa
     4741 cgagagtaag aatcaacgta gccaatgcta tcggctccca tgctatgata taatgcaggc
     4801 gttcaatact accacctgta atatgagccc tgatagggaa tagtagtcct gcaatataaa
     4861 atagaactcc cattaaacta acgataatga ttccatcctt aatacgctct tcccagctat
     4921 aaataaattg ttttttatag agagttggct taatgactaa taagcctaac aatagtaaaa
     4981 taaaccagaa agatagtcgc caactaatat gacctaaatt aagataaaac aatgtcaaag
     5041 taatccagat aatagcaatt ggaaaagcat ttttcacttt ttgatcaata gttctagcta
     5101 agagaataaa acaaaccccc agcaataaac ctggaaactg ccataacatt tgttcttgta
     5161 atgggtccaa gcctagcttc tgcaa
//
LOCUS       DATRNX010000121         5079 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRNX010000121 DATRNX010000000
VERSION     DATRNX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            34..1119
                     /locus_tag="VBC81_001772"
     CDS             34..1119
                     /locus_tag="VBC81_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6912788.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            1144..1269
                     /locus_tag="VBC81_001773"
     CDS             1144..1269
                     /locus_tag="VBC81_001773"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO6912789.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            1334..2143
                     /locus_tag="VBC81_001774"
     CDS             1334..2143
                     /locus_tag="VBC81_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6912790.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            2140..3051
                     /gene="pfkB"
                     /locus_tag="VBC81_001775"
     CDS             2140..3051
                     /gene="pfkB"
                     /locus_tag="VBC81_001775"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6912791.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            3048..5012
                     /locus_tag="VBC81_001776"
     CDS             3048..5012
                     /locus_tag="VBC81_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6912792.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         1676 a          765 c         1037 g         1601 t
ORIGIN      
        1 gagaaaagta ggaaatagtt aagaaaggta tttatgacgc ttcgagaatt aacaatagaa
       61 gaatttaaag aacattcagg aaattatgat tcacaatcat ttttacaaac acctgagatg
      121 gctaaacttt tagaaaaacg cggctatgat gttaggtatt tgggatatca agtagaaaat
      181 aaactagaga taatcagttt atcttatatt atgccagtca ctggtggttt tcaaatgaaa
      241 attgattcag gaccagttca ttcaaattct aagtatctaa aacaatttta taaagcattg
      301 caaggctatg ccaaatccaa cggtgttcta gaattaatag ttgagccttt tgatgattac
      361 caattattca ctagttcggg agttcctagt aatcagggaa atgataatct gattgaagat
      421 tttaccagtt caggttatca ccatgatggt ttaacaactg gttttactgg taaatattta
      481 tcttggcact atgttaaaaa tttagaaggt gtcacttctg aaacgttact atcttcattc
      541 tctaagacag gacgagcttt ggttaagaaa gcaatgtctt ttggaatcaa ggttcgcgtt
      601 cttaaacgtg atgagctaca tttatttaaa gagataacaa cttctacgtc aaatagacgt
      661 gattatatgg ataagtcctt agattattat caagattttt acgatagctt tgaaggcaag
      721 gctgaatttg tgattgccac tttaaatttt agagaatacg accataactt gcaaataaaa
      781 gctgaagcat tggaaaataa gcttaaattg cttgatgaac gttttaggga aaatgcagac
      841 tctcctaagt atcataggca acgctctgaa ataattaatc aactggctag ttttgaaaca
      901 cgacgacaag aagttcagtc atttattcaa aagtatgata accaagacgt tgttttagct
      961 ggtagtctat ttgtttatag tcttaaggag acagtttatt tcttttcagg atcctacaca
     1021 gaatttaata aattttatgc accagcagtt cttcaggaat atgttatgca agaggctctc
     1081 aaaagaggtt ctactttcta taacctccta ggtatttagg gaacttttga tggttcagat
     1141 agtattcttc gctttaaaca gaattttaat ggttatatca ttcgtaaaat gggaactttt
     1201 aactattatc caagtccttt taaatacaaa ggaattcagc ttcttaaaaa ggttttgaag
     1261 agaaattaaa gtatttgtaa aagctaatct ttagattagg cttttttagt ttattgatag
     1321 tttttggaag aaaatgcttg acattgaaag aaaaagagat ataattaaat tgcaaacgat
     1381 ttcaattcaa ggagggacta tgctaaagtc aaagagaaaa gaaattatct tatcacgact
     1441 tgagcaaaat aagagtgtta ctttagatga attaacgtct atcttagaga cttctgaatc
     1501 tacagttcgc agggatttag atgaacttga atctgaaggt tttttaaaaa gggtgcatgg
     1561 tggtgctgaa ttaccatact cacttggcca ggaattaagt aatcaagaaa aatctatcaa
     1621 aaacgttcaa aaaaaattgg atattgctag acaaactgca aagctgatag caaagcaaga
     1681 tgttatattt attgatgccg gaactacgac agaattatta attgattttc tccctcacga
     1741 gcaattaacg gtggtgacga attctattca tcatgctgca aagttagttg atagagggat
     1801 aaaaacaatc attatcggag gagcggttaa acattcaaca gatgctagta ttggccaggt
     1861 agccattaat cagattaggc agataacggt tgacaaagct tttctgggga tgaatggtat
     1921 agatgaagtt tatttaacta caccagattt agaagaagct gctattaaag aagctattat
     1981 taataatagt caacaaacct ttattctaat ggattcatct aaaattggac aagttacatt
     2041 tgctaaagtg aaagagataa atgacattaa tttagtaact aataagactg attcagaact
     2101 tatgacaata ataaaagaga aaatgaaggt gattcaagta tgatttatac agtaacatta
     2161 aatccatcga ttgattttat tgttcgtcta gatacactat tgttgggtag tgttaaccgt
     2221 atgactagcg atgataaata tgtaggtggt aaaggaatta atgttagtcg catattaaaa
     2281 cgcttaaaaa ttgacaatac tgctacaggt tttatcggtg gatttacagg acattttgtt
     2341 gaagatggac ttgtatcaga aggtatcaaa actgactttg tttctgttaa tgaagataca
     2401 cgtatcaacg ttaaagtaaa agcaaaaatt gaaactgaaa tcaatggcgg gggtcctagg
     2461 ataacaaatg aacaacttca tcgtctagag aaacttcttt cgagactaac acctgaggac
     2521 acagtagttt tcgcaggttc agcaccagct agccttggaa ataaggtcta taatacattg
     2581 attccaattg ctaaaaagac aggagcggaa gttgtttgcg attttgaagg tcagacactt
     2641 ttagacgctc ttgcttatca gcctctttta gtaaaaccga ataaccatga attagcagat
     2701 atttttggcg tagagctaga aggtctgcct gatatcgaaa aatatgcaca taagattcta
     2761 gataaaggag ctaagaatgt tatcgtttct atggcagggg atggtgcatt gttagtaact
     2821 ccagaagctt catattttgc aaaaccgatt aagggtgaag tgaaaaattc cgttggggct
     2881 ggtgattcaa tggtagctgg ctttacagga gaatttgtta agtcaaaaaa tccagtagaa
     2941 gcactaaaat ggggcgtagc ttgtggtaca gcaaccactt tctcagatga tttagcaaca
     3001 gctgagttta tccaagatat ttataataaa gttgaggtag aaaaattatg aaaatccaag
     3061 acctactaaa aaaagaagtt atgattatgg atttaaaagc aaccagtaaa gaggctgcaa
     3121 ttgatgaaat gattacaaaa ttagttgaca caggtgttgt gacaaacttt gctattttta
     3181 aagatggtat catgaagcgt gaggcacaaa catcaactgg attaggtgat ggtatcgcta
     3241 tgccacatag taaaaatgct gccgttaaag aagctactgt cctttttgca aaatctgcat
     3301 caggtgttga ttacgaagca cttgatggac agccaactga tttattcttt atgattgctg
     3361 cgccagatgg tgctaatgat acgcatttag ctgccttggc agaactatct aaatatcttt
     3421 taaaagaagg gttcgctgat caattacgtc aagcaaaaac gccagatgac attattgcta
     3481 cttttgatag taatagcatt tctcaagaaa cagtagctcc acaaacagta caatcaacaa
     3541 gtaaaggttc agattatatt gttgcagtaa cagcttgcac tacgggaatt gcccacacgt
     3601 atatggcaga agaagcttta aagaaaaaag cagctgaaat gggtgtaggt attaaagttg
     3661 aaacaaatgg cgcttctggt gtaggaaata agttgacttc aagtgatatt gcaagggcta
     3721 agggtgttat tattgcagca gataaggctg tagagatgga tcgttttgat ggcaaacctt
     3781 tggtatcacg tccggtagct gatggtatta aaaaaagtga agacttaatt aacattattc
     3841 tcgataataa ggcacaaacc tatcatgcaa aaaatcaaaa tgataaacaa tcaggtgaat
     3901 ctgatggaaa atcaggctta ggctctgctt tttataaaca ccttatgggt ggtgtatcac
     3961 aaatgttacc attcgttatt ggtggtggta ttatgattgc gattgctttc ctttttgata
     4021 acatacttgg tgtaccaaaa gaccaattaa gtaatttagg ttcataccat gaaattgcag
     4081 ccttattcaa aaatatcggt ggcgcagcat ttgcatttat gttaccagta ttggcaggtt
     4141 atatcgctta ttcaatagct gaaaaaccag gactagtagc tggcttcgta gctggttcaa
     4201 ttgcatcaag tggactggca ttcggtaaag taccttttgc agaaggtggg aaagcaacct
     4261 tagcattagc aggtgtgcca tcaggtttct taggggcttt agtaggtggt ttcttagctg
     4321 gtggagttat cctactactt agaaaattac tttcaggtct cccaaaatcg cttgaaggta
     4381 ttaaatcaat tcttctatac ccattgttag gtgttttgat tacaggtttc ttgatgttgc
     4441 tcgtaaacat tccaatggca gctatcaaca cagctcttaa cactttctta caaggtttat
     4501 ctggtagttc agcagtcttg atgggcttac tagttggtgg tatgatggct gtagatatgg
     4561 gtgggcctgt taataaagca gcttatgttt ttggtacagg tactcttgca gcaactgttg
     4621 caaatggcgg ctctgttgtt atggcagctg taatggctgg aggaatggtt ccaccgttag
     4681 cagtatttgt ggcaacttta ttatttaaag ataaatttaa taatgaagaa cgtcaatctg
     4741 gtctaacaaa tatcgttatg gggttatctt ttatcactga aggtgctatt ccgtttggtg
     4801 ctgctgaccc agctcgtgca atccctagct ttattgtagg ttcagcctta actggagcac
     4861 ttgttggttt agctggtatc aagttgatgg ctcctcatgg agggattttt gtcatcgcat
     4921 taacaagtaa tcctttactt tatattctat ttattctaat tggagcgata gtctcaggtg
     4981 tactctttgg cttgttccgt aaaacaaaat agttaatctc aacttatcca aatttacatt
     5041 gaataaaagg tgaataaact atcgttattg ggtctattc
//
LOCUS       DATRNX010000122         4999 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRNX010000122 DATRNX010000000
VERSION     DATRNX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            98..1646
                     /locus_tag="VBC81_001777"
     rRNA            98..1646
                     /locus_tag="VBC81_001777"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VBC81_001778"
     tRNA            1701..1773
                     /locus_tag="VBC81_001778"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            1928..4830
                     /locus_tag="VBC81_001779"
     rRNA            1928..4830
                     /locus_tag="VBC81_001779"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4905..>4999
                     /gene="rrf"
                     /locus_tag="VBC81_001780"
     rRNA            4905..>4999
                     /gene="rrf"
                     /locus_tag="VBC81_001780"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1410 a         1015 c         1445 g         1129 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
      541 gttgttagag aagaacgttg gtaggagtgg aaaatctacc aagtgacggt aactaaccag
      601 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
      661 ggatttattg ggcgtaaagc gagcgcaggc ggttctttaa gtctgaagtt aaaggcagtg
      721 gcttaaccat tgtacgcttt ggaaactgga ggacttgagt gcagaagggg agagtggaat
      781 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
      841 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
      901 tggtagtcca cgccgtaaac gatgagtgct aggtgttagg ccctttccgg ggcttagtgc
      961 cgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
     1021 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
     1081 aaccttacca ggtcttgaca tccttctgac cggcctagag ataggctttc tcttcggagc
     1141 agaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1201 ccgcaacgag cgcaacccct attgttagtt gccatcatta agttgggcac tctagcgaga
     1261 ctgccggtaa taaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac
     1321 ctgggctaca cacgtgctac aatggttggt acaacgagtc gcaagccggt gacggcaagc
     1381 taatctctta aagccaatct cagttcggat tgtaggctgc aactcgccta catgaagtcg
     1441 gaatcgctag taatcgcgga tcagcacgcc gcggtgaata cgttcccggg ccttgtacac
     1501 accgcccgtc acaccacgag agtttgtaac acccgaagtc ggtgaggtaa ccttttagga
     1561 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg
     1621 gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa acctgccatt tgcgtcttgt
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccattgaatc gaaaggttca aattgttcat
     1801 tgaaaattga atatctatat caaattccac gatctagaaa tagattgtag aaagtaacaa
     1861 gaaaataaac cgaaaacgct gtgaatattt aatgagtttt ctagttttaa agaaactagt
     1921 ttaataaggt taagttaata agggcgcacg gtggatgcct tggcactaga agccgaagaa
     1981 ggacgtgact aacgacgaaa tgctttgggg agctgtaagt aagcgctgat ccagagatgt
     2041 ccgaatgggg gaacccacta gttaatgact agtatccata tctgttaagg atatgagaag
     2101 gaagacgcag tgaactgaaa catctaagta gctgcaggaa gagaaagcaa aagcgattgc
     2161 cttagtagcg gcgagcgaaa cggcaggagg gcaaaccgaa gtgtttacac ttcggggttg
     2221 taggactgcg atataggatt aatcattata gaagaattac ctgggaaggt aagccaaaga
     2281 gagtaacagc ctcgtattta aaatgataga ttaaccttag cagtatcctg agtacggcga
     2341 gacacgcgaa atctcgtcgg aatctgggag gaccatctcc caaccctaaa tactctctag
     2401 tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga
     2461 aatagaacct gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg
     2521 ccttttgtag aatgaaccgg cgagttacga tatgatgcga ggttaagttg aagagacgga
     2581 gccgtaggga aaccgagtct taatagggcg ccatagtatc atgttgtaga cccgaaacca
     2641 tgtgacctac ccatgagcag gttgaaggtg aggtaaaact cactggagga ccgaaccagg
     2701 gcacgttgaa aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga
     2761 gatagctggt tctctccgaa atagctttag ggctagcgtc gatgttaagt ctcttggagg
     2821 tagagcactg tttgggtgag gggtccatcc cggattacca atctcagata aactccgaat
     2881 gccaacgaga tataatcggc agtcagactg cgagtgctaa gatccgtagt cgaaagggaa
     2941 acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag gatgtggggt
     3001 tgcacagaca actaggatgt tagcttagaa gcagctattc attcaaagag tgcgtaatag
     3061 ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg ctaaaacata ttaccgaagc
     3121 tgtggatgac acaaaagtgt catggtagga gagcgttcta tgtgtgaaga aggtgtaccg
     3181 tgaggagcgc tggaacgcat agaagtgaga atgccggtat gagtagcgca agacaggtga
     3241 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     3301 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     3361 tagagtatat agtgatggag ggacgcagta ggctaactaa accagacgat tggaagtgtc
     3421 tggtcaaaca gtgaggtgtg atatgagtca aatgcttata tctttaacat tgagctgtga
     3481 cgaggagcga agtttagtag cgaagttagt gatgtcacac tgccaagaaa agcttctagc
     3541 gatacatata ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag
     3601 gtgagcgaga gaactctcgt taaggaactc ggcaaaatgg ccccgtaact tcgggagaag
     3661 gggcgctgat tttagatcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac
     3721 agctctctgc taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg
     3781 ttaagaggag tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg
     3841 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
     3901 aggcgtaatg atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga
     3961 agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata
     4021 ttgagtatct gtaccacatg tacaggatag gtaggagcct atgataacgg gacgccagtt
     4081 tcgtttgagg cgttgttggg atactaccct tgtgttatgg ctactctaac ccagataggc
     4141 aatccctatc ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag
     4201 gtaacggagg cgcccaaagg ttccctcaga ctggttggaa atcagtcgta gagtgtaaag
     4261 gtataaggga gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt
     4321 gatccggtgg ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata
     4381 acaggcttat ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc
     4441 tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg
     4501 cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag
     4561 gaaatttgag aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta
     4621 ccagttgtct tgccaaaggc atcgctgggt agctatgtag ggaagggata agcgctgaaa
     4681 gcatctaagt gcgaagccca cctcaagatg agatttccca tgattttata tcagtaagag
     4741 ccctgagaga tgatcaggta gataggttag gagtgtaatt gtggtgacac atttagcgga
     4801 ctaatactaa tagctcgagg acttatccaa aaagagatat tgacaacgtt acggtttctt
     4861 gttagactat agatattcaa ttttgagtga ataagactca atagttaagt gataattgcc
     4921 taggagatac acctgttctc atgccgaaca cagaagttaa gccctagaac gccggaagta
     4981 gttgggggtt gccccctgt
//
LOCUS       DATRNX010000123         4878 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.124, whole genome shotgun sequence.
ACCESSION   DATRNX010000123 DATRNX010000000
VERSION     DATRNX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            38..682
                     /locus_tag="VBC81_001781"
     CDS             38..682
                     /locus_tag="VBC81_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6912793.1"
                     /translation="MPLLIHSLILSVLPFLMWLTLVNRDKPLYKTIWSILLGLQLITI
                     YTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGVVGGVLAMIHPDFYP
                     YQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLTLLINMSLIFINLLT
                     GGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVRMRASFKTEES"
     gene            862..2399
                     /locus_tag="VBC81_001782"
                     /pseudo
     CDS             862..2399
                     /locus_tag="VBC81_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            2576..4120
                     /locus_tag="VBC81_001783"
     CDS             2576..4120
                     /locus_tag="VBC81_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6912794.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            4206..4586
                     /locus_tag="VBC81_001784"
     CDS             4206..4586
                     /locus_tag="VBC81_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6912795.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4780..>4878
                     /locus_tag="VBC81_001785"
     CDS             4780..>4878
                     /locus_tag="VBC81_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912796.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVG"
BASE COUNT         1594 a          741 c          959 g         1584 t
ORIGIN      
        1 agaattcttt tctaatatca gaacagagat tccgcagatg cctttactta tccatagttt
       61 gattttatct gtcttacctt ttctgatgtg gctgactttg gttaatagag ataagccttt
      121 gtataaaact atttggagta tccttttagg acttcagtta attacgattt atacttggtt
      181 tttctgggca aaattgcctt tatctgaaag tcttcccctt taccattgtc gaataggcat
      241 gtttgtcgtt ctcttagcga ggcctagaat attaaaggat tatttcgcat tgttaggagt
      301 agtaggtgga gtattggcaa tgattcaccc agacttttac ccttaccagt ttctacatgt
      361 gactaatatt ttcttcttta ttggacattt tgctttattt gtccttagtt tactacatct
      421 gatgacacag tccaatttgg acaaattaaa tccaaaattg attatacaac taacgcttct
      481 cattaatatg tctttgattt ttattaacct cttgactggg ggaaattatg gctttatgat
      541 gaagacacca atactaggaa taacaaatcc ctttttaaac cttttgatag tgacaactct
      601 attaagtttt ctcgtcctat ttgttaaaca aattttccaa aaagtgagaa tgagagctag
      661 ttttaaaaca gaagaatctt aattatttta atattattat attatctagc tattaaaaaa
      721 taaatagctg gataatgttt ttcattttta gctattttta tgattgaccg ctaattatta
      781 atctgctaag atatagtacg taataaaaac ttaaccggtt aattattttt tgaaatatat
      841 tttttctgga ggaaaatagt aatgaataaa tcattcaata ccaaattagg tttggtaact
      901 gtagcggcat tatcaggtat tgtattaacg tctcaattac ctgtgaacgc taaagctgag
      961 acaccagtaa tggcagcaag tgcacaacaa ggtttccgtt ttgtagcaac cgttgtagat
     1021 agtcagacgc gtgtcttgcc gggaaaattg gtaactttgt cagaggtgac aactggtcag
     1081 cctaaaatta ttgcaagtgt aaaaacgaat gatgccggac aagctatttt taataatctc
     1141 cctataaaac gtaaccttag tgtgtctgtt gatggacaag taaaaggata tactattcga
     1201 actgacgtag caggctctag taaagcagct agttttacag caacaggagt agggacaaat
     1261 gagccaacgt attcaaaaaa aaccatagac attacagtga gagaccaaaa tgctgaacct
     1321 gtttcaggca ggacagttac cttaaaaaca catgctggtc gtgagattgc tagcttggtt
     1381 tctggtgaca atggattaac tcgctttact gatcgtttac tagatggaac attctaccaa
     1441 tattttgttg atgggaaaaa attggtgata ttgtacctgg agaatcaaga agtgcatatg
     1501 ttaatgttgc acctaaaaaa gattatttta cctttacagt cactgcttta gataaagatg
     1561 gacttgttgt taagggaaaa gaagtaacgt tgactgatat tactgatggt aaagcagtag
     1621 cgcttgcttc tttaaaaacg aatgataatg gacaagctat ctttaccaac ctaccattat
     1681 caagaaatat cagtgtttca attgatggaa aatcaaaggg ctatactctt cgaactgatg
     1741 tagcaggctc tcataaagcg gctgcattct atgtagatgg taaaggtacc aaagcacctc
     1801 aatttagtgc agaagcagcg gttgtaacta tttacgatgc taatggaaat actatagcaa
     1861 accaagaagt gacattaact aattcaaatg ggacaattgt tgcaaaaggt ttgactgaaa
     1921 aagatggcaa agcaaggttc gccaataaat taatgggagg aacgttatac aatatttttg
     1981 taaatggtat tgagatgcct aaaactgccc ttgttggtag tgatgtcagt gtttttctaa
     2041 cagataaaca aattaagaaa gaaaaacctg ttactcctga taaattggat aaagaaatga
     2101 accaagagaa ggaaaaggag ctcttagata ctccaaaacc agtaccacct acttcgaaac
     2161 caacagcacc aattaaaaat aaaaaggacg attcccctaa gaagtttacg aaaagtcaac
     2221 cttctagttt gaagtcaagt gttaaggcta taggtgctaa gccaaaagct atgacagcct
     2281 tagctaaaaa attgcctaaa actggtgatc aagccattag tattttaact ttggttggtt
     2341 ttattgtaac agggttagcg attatgttag gcttctttaa aaaatcacga tcaaaataat
     2401 ttattttaga agcttgggtc tttagacaca ggcttctctt ctttcttcgg aaatagattg
     2461 aacttgaaga atgttgttaa attcgctata ataataaagc tatagacaag attattgttt
     2521 cagttgagac tactgaaact aatactaaga aatggataaa agctaaggat tgaatatgac
     2581 attacaagac gaaatcaaaa aacgccgtac ttttgccatc atctctcacc cggatgctgg
     2641 taagacgact attactgagc aattattata ttttggtggt gaaattagag aagcagggac
     2701 agtaaaaggg aaaaaatcag gtacttttgc aaagtccgac tggatggata ttgaaaagca
     2761 acggggtatc tctgttactt catctgttat gcaatttgat tacgcgggta aacgtgttaa
     2821 tattttggat acacctggac atgaggattt ctcggaagat acataccgga ccttaatggc
     2881 tgtggatgca gctgtcatgg ttgtagattc ggctaaaggt attgaggctc aaactaaaaa
     2941 actttttgaa gttgttaagc atcgtaatat tccggttttt acgtttatta ataaattgga
     3001 ccgtgatgga cgtgagccac tggatttgct tgaagagtta gaagaagtac taggtattgc
     3061 ttcttatcca atgaattggc caattggtat ggggaaatca tttgaagggc tttatgattt
     3121 acataataaa cgtttagaac tctataaagg agacgagcgt tttgcaagta ttgaagacgg
     3181 tgatcagctg tttgccaata atccatttta tgaacaagtc aaagaagata ttgaacttct
     3241 tcaagaagca ggtaatgact tttcagaaca agctattctt gatggtgacc taactccagt
     3301 attctttggc tctgccctta cgaactttgg agttcagact tttcttgata ctttcttgga
     3361 gtttgctcca gaacctcatg gtcacaagac tacagaaggt aatgttattg accccttagc
     3421 taaagatttt tcaggttttg tctttaaaat tcaagctaac atggacccac gtcatcgcga
     3481 ccgtattgct tttgttcgta ttgtttctgg tgaatttgag cgcggcatgg gagttaattt
     3541 gacacgcact ggtaaaggcg ctaagctctc caatgttact caatttatgg cagaatcacg
     3601 agaaaatgtg acgaatgctg ttgctgggga tattatcggg gtttatgata caggaactta
     3661 tcaagttggg gataccttaa cggttgggaa aaataaattt gaatttgaac cattaccaac
     3721 ttttacccca gaattattta tgaaagtatc agcaaaaaat gttatgaagc agaaatcatt
     3781 ccacaagggt attgagcaat tagtacaaga aggagcaatc cagctttata agaattatca
     3841 gacaggcgaa tacatgctgg gtgctgtggg tcaacttcag tttgaagttt ttaaacatcg
     3901 tatggaaggt gaatacaatg ctgaagttgt tatgacacca atgggtaaaa agactgtacg
     3961 ttggattaac tcagacgatt tagacgaacg catgtcctca agccgaaata ttttagcaaa
     4021 agatcgtttt gaccaaccag ttttcctatt tgaaaatgat tttgcacttc gctggtttgc
     4081 tgataagtat cctgatgtga agcttgaaga aaaaatgtaa aatttaaagt cactcaaatt
     4141 tgagtgactt tttaagtttt ttagactaaa atagttctaa tacatagaaa agattggaga
     4201 ttaacatggg aattttttcg ggattaatgg gaaatgcttc acaagtggat accgataaag
     4261 tcgagaatca gttatctgat attctgattc caggtgaaca ggttgattta gcttatactt
     4321 taattcgtga tttgattgtt tttactaatt atcgtttaat tttagtcgat aaacaggggg
     4381 tgacaggtaa aaaggtttct tataattcta taccttatgc ttctatatca cgttttacag
     4441 tggaaactag tggtcatttt gatttagatg ctgagttaaa gatttggata tcatcagcaa
     4501 tagaaccagc agaagtactt cagtttaaga acgaccgtaa catagtgagt atacagaaag
     4561 ctttggcgat agctgtttta aaataaacaa tttagcaagt gctgctacta agagttattt
     4621 gttttaatta taacttttaa tagtgctaaa ttctcatttt caaaataaaa gtagtgattt
     4681 ttatcactgc ttttattttg aagtatagta ggcctcaagc atatattttg aaaacgctgt
     4741 atttttttga taagatattt ttttgaggag tattaaataa tgataataga attaaagagt
     4801 gttgggaaaa tttttccaaa atcacagttt agattagatg atatttcttt taatatagca
     4861 gaaggcgaga tagttggt
//
LOCUS       DATRNX010000124         4753 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRNX010000124 DATRNX010000000
VERSION     DATRNX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..796)
                     /locus_tag="VBC81_001786"
     CDS             complement(<1..796)
                     /locus_tag="VBC81_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6912797.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGNI
                     KAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQKIS"
     gene            complement(810..1439)
                     /locus_tag="VBC81_001787"
     CDS             complement(810..1439)
                     /locus_tag="VBC81_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6912798.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(1449..2090)
                     /locus_tag="VBC81_001788"
     CDS             complement(1449..2090)
                     /locus_tag="VBC81_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6912799.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(2225..2554)
                     /locus_tag="VBC81_001789"
     CDS             complement(2225..2554)
                     /locus_tag="VBC81_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6912800.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(2717..4531)
                     /gene="glmS"
                     /locus_tag="VBC81_001790"
     CDS             complement(2717..4531)
                     /gene="glmS"
                     /locus_tag="VBC81_001790"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6912801.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT         1546 a          927 c          688 g         1592 t
ORIGIN      
        1 aagagatttt ttgaaatctc cccttattat gaagctgttt gaaagctttg ttaatctttt
       61 caattagtct acgatcaact ttacgagctc ctactacaaa attttctcct tgataagcag
      121 ttttaacaaa ataataagct tttatatttc cttcttgctt taaataatag ttagcataaa
      181 cttcatcaat caaaagacca tcaatacggt tattttttaa atcaatcaaa gcctgagtga
      241 aagtatcgta ttgaactgct tcttttcctt ttacaaactt ttttaaaata tcaggtttag
      301 cgttaaaagc atcaaaacca gatgaacccg actgggctcc tagttttttc cccttcatat
      361 ccttaatact attaatatgt gatgaagttt tagtaacaat tacttgatga ttattcatat
      421 atgggtttgt aaaagcgact tttttagcac gttctgccgt ttttgaataa ccattccaaa
      481 taaggtctat attaccatta ttaagttcag tttctttcat atcccagtta ataggctgcc
      541 atttcactga aataccgtat tctttaaaaa cagcattagc taaatcaata tcaaagccgg
      601 tatagtcacc agaacgactt tcaaatccca taggaacaaa agtattatca aatccaatag
      661 taattttctt ttccttttga taatgttccc aattatcaat accagccatt tccttattag
      721 acataccaca tgctgataat ataatcataa ataatccaaa tataatggtt aataatatat
      781 ttttgtgagt caacaatcta cccccaagtc tacttaggat taattttgag aatcgtatca
      841 gatatacttt cagcaaattg aagatcatgt gttactacaa tttgtgtcat acctgtttct
      901 ctattttgta aaattagttt ttctacttct tgacgcaact ctggatcaag agcgctagtt
      961 ggctcatcat aaccaataat ctgtggatca atcatcatcg cacgagctag tgcgacacgt
     1021 tgcttttgcc ccccagataa tgaaaatgga tagactaaag catggtcttt aagtcccaag
     1081 cgttcaagta atactaaggc tttttcttta gcaagttctt ttgataatcc catcgtttta
     1141 acaggagaga ggatcaaatt atctaatact gttaaatgtg ggaataattg aaagtcttgg
     1201 aagacgaatc ctaaaagatt tagtgtttcc aaatgatcaa cagatacttc tttaccatcg
     1261 tgaacaatag tccctgaatc aatcttttcc aatccagcta acatacgtaa tagtgttgtt
     1321 tttccacctc cagaaggtcc tactaaagag agaattttac cttcttctac agtcagattg
     1381 aaatctttaa agatttcttt ttgcccatag catttagaaa tattttttaa ttctaacatc
     1441 gtccctcctt atttatagta gttaaaacgc ttttcaactt gtttagataa aatagttaaa
     1501 agaccaatta ataataggta aataccacct gctataaaca ttggtgcaag tgttgcatcc
     1561 ctatttgcgg ctgtcttact ttctagaagt aagtcgccca caccaagtat gtaaaccaat
     1621 gaggaatctt tcactagagt aatcgtttca ttgaaaacac tcggtaagac tattttgaag
     1681 acttgaggta atactatgta ccaaaccgtt tgaaacttag aaaactttaa tacctttgca
     1741 gcttcatatt gccctttagg aatagactca atacctcctc gaaaaatctc tgcaaaataa
     1801 gctgcataat tcatcgtaaa agcaataatc gcagctggca tacgatcaaa agaaattcct
     1861 acacttggta aaaagtaata aaaaaagata agctgtaata acagaggcgt gcctcgcata
     1921 atccaaatat aagttgtcag tagccattga agcggcttaa aatgaacttt cattaggaag
     1981 gctacaacaa taccaagtgg aattgataaa caaataacaa taaagaaaac ttgcaatgtc
     2041 acttgcgcac ctactaataa gctaggtaag atttctaaaa tataagacat ggtcccccct
     2101 aaataaaaaa tagatgttct cattatatca aaattcaaac caatgtcata gagctttttt
     2161 aataaaaatg catgaaatca agtcatattc aaaaagcaat tgaccgtcgc caattgcctg
     2221 aaagctattt ttttataaac tcagatttta gtttcatagc tccaaaacca tcaattttac
     2281 aatcgatatt atgatcgcca tcaactaaac gaatattttt aacacgagtg ccttgcttaa
     2341 tatccttagg cgctcctttt acttttaaat ccttaataac tgtcacagta tctccatctg
     2401 ataagcgcgt tccattgcta tccaatacaa ttaaacctac ttcttcttcg atttcttctg
     2461 gattccactc gtaagcacat tctggacaaa ccagtaaaat accgtcttca tagacatatt
     2521 cagagttaca cttagggcaa tttggtaatg acatttttct ctccttaaat taattaataa
     2581 aatagtcagc aagtaacatt ctaactgatt ataggcttct tgacaatagg aaaaagccta
     2641 ttttccttca aaaataggct tttttacaat ttttaatatt ttgtagttaa tattagcgta
     2701 ttttagtgta acgagtctat tcaacagtta cagcttttgc caaattacgt ggtttatcaa
     2761 catctaagcc acgttgtaat gaagcatagt aagcaattaa ttgagtcgga ataaccattg
     2821 aaatggctga taagaatggg tgaactttat taacaataat atcatcacct tcacgttcca
     2881 acccttcttc tacaataatc aaagcatttg ctccgcgaga aacaacttct tggatatttc
     2941 cacgagtatg cgctgcaata gttgaatcag cagatattaa agcaataaca ggagtattgt
     3001 cttcaatcaa cgaaattgta ccatgtttta attcacctgc agcaaaacct tctgtctgaa
     3061 tataagaaat ctcttttagc ttcagtgcag cctccattgt aacataatag tcgttaccac
     3121 gaccaatgta gaaggcatta cgtgtcgaga ttaacaactg ctctaccttc tcagagataa
     3181 catctttttc tgataaggtc gcttcaattg attgtgctac aattgacaat tcatgaacca
     3241 aatcaaaatc ttttgcttct gccttaccat ttgcttctcc taccgctttt gctaagaaag
     3301 ctaacgttgc aacttgagca gtatatgctt ttgtagaagc aacagcgatt tcaggtcctg
     3361 catgaatgag catcgtatag gttgcttcac gagaaagtgt tgagccaggc acatttgtta
     3421 tagttaaact agggattccc atttcgtttg ctttaactaa aacttgtcta ctatctgctg
     3481 tttctcccga ttgactaagt aaaataaaca ttggcttctt actcaaaagt ggcatgttgt
     3541 aaccccattc tgaagaaact ccaagttcaa caggtgtatc agttaatttt tcgatcatgg
     3601 tcttagcagc aaaaccagca tgatatgacg ttcctgcagc caatatgtaa agacgatctg
     3661 cttcttggac tgattttata atatcagaat ccacattcat atctccagat tcgttcgcat
     3721 aagttgaaat caacttacgc atcacagttg gttgctcatc aatttcttta agcatgtaga
     3781 atggataagt ccccttacca atatctgata agtcaagctc tgctgtgtag ctaccacgtt
     3841 caatgacatt accatcataa tcttgaactt caacgctatc ttttttaaca ataacgagtt
     3901 ctttatcgtg gatttccata tattcacttg tctcacgtat catagccatg gcatctgaac
     3961 acaccatatt ataaccattt cccaaaccta ttagaagagg tgatttattt ttagctacgt
     4021 aaatcgtatc agcatcttga gaatcaataa gagcaaatgc gtatgatcct tctataatat
     4081 gtaaggcttt tttaaaggct tcaagcactg ataagttatc ttcttcaaca aagtgttcaa
     4141 ctaaatgaat agctatttct gtatcagttt cacccttgag gttatgtttt gttaagtaag
     4201 tttctttaat ttgaaggtaa ttttcaataa caccattgtg taccaataca aaacgaccag
     4261 atcctgaagt gtgaggatgt gcatttcctt cagtaggttt accatgggtt gcccaacgtg
     4321 tatgaccgat acctgtcgta ccactaacag aatctcctac ttttgcctga agctccgcga
     4381 ttcgaccgac tgacttcaca agttgacttt tgttatcacc tactacaaaa atacctgctg
     4441 agtcatatcc tcggtactct agcttttcta agccttgaat taaaatatcc gttgcatttg
     4501 tatttccaac tacacctacg ataccacaca tattattatt accaataaga gctttctctt
     4561 atactttcta tctaaacttt ctttttgaat tggtatatat taatatatac cgttaaaaag
     4621 taacaaaaac agtatataat taatgttttt gtttgtcaat aaaaaaattg gtatagtctt
     4681 tttgattttc tgtgataacg agataaacgc tatcaaaaat tctatctatc ttaaaaaaat
     4741 atatttctta gcc
//
LOCUS       DATRNX010000125         4477 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRNX010000125 DATRNX010000000
VERSION     DATRNX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..322
                     /locus_tag="VBC81_001791"
     CDS             <1..322
                     /locus_tag="VBC81_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912802.1"
                     /translation="AHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMI
                     ILERANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITG
                     IKTK"
     gene            976..4416
                     /locus_tag="VBC81_001792"
     CDS             976..4416
                     /locus_tag="VBC81_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6912803.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGNAPSEMKEPYRLEGAMPEA
                     QLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAFD
                     YAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLT
                     ETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIERGDI
                     DFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNPPKTI
                     TFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTS
                     MSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFSPRQQ
                     GAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQVTV
                     QTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFL
                     EGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQL
                     DGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQD
                     DDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNVVWTS
                     EVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKE
                     QHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDEDLPTT
                     EYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNK
                     PEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSSRTLE
                     KRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQKETKK
                     "
BASE COUNT         1569 a          886 c          882 g         1140 t
ORIGIN      
        1 agctcatctt agaaatcttt caaatcgtgg tattggtatc atggaatcac aacacagaaa
       61 gataacgtat agaatgaagc gacgtggcat gtattggtca aagtggggaa tctccacaat
      121 ggcaaatatg attatacttg aaagagctaa cggtttacga gaattatttt tcggttcttg
      181 gagaaaggta tacagtgagt ataaagaagg ttcatttagt gcagggcgac tttttaaaaa
      241 gacagatgaa ttagataaat tttctaagcc ccttctaaaa aatggcagaa aatggagtat
      301 aacaggaatc aaaacaaaat agaaaaagtc taaagatacc tttatttcta gcgttttttt
      361 gcatgtttta acttgcatta aatcttctaa agtagtataa ttatattaaa gaaaagcaaa
      421 aaaggtgaag gagcgcgaac tcctacacca aaccgattaa gacaaatacc acttgcttaa
      481 tcagtcgaac cttattgtag cataactaaa acaataccgt caagactgaa caagcaaaaa
      541 atacttgttt tggtcttttt aagaataatt caagttttct taaatttatg aggtatcgac
      601 taatctcaaa atttcagaca catacctgag tgaacatcaa gagagaaccg atcggttctc
      661 tcttgatgtc taaaagaatg aggttaagga ggtcacaaac taaacaactc ttaaaaagat
      721 gacctttact aataatcgtc tttgttttat aataaagatg ttagtaatat aattgataaa
      781 tgagatacat ttaatcatta tggcaaaagc aagaaaaata gctgtgtcat atgcaaataa
      841 cccctgtttg ctctttaaaa aagacgttat ccttatttct ctacacacag acggatagct
      901 aggagagaat cgtttgattc tctcttttct taatggtcat aaagacaaag tctctcatca
      961 tgaaaggacg acacattgcg taaaaaacaa aaactaccat ttgataaact tgccattgcg
     1021 cttatatcta cgagcatctt gctcaatgca caatcagaca ttaaagcaaa tactgtgaca
     1081 gaagacactc ctgctaccga acaagccgta gaacccccac aaccaatagc agtttctgag
     1141 gaatcacgat catcaaagga aactaaaacc tcacaaactc ctagtgatgt aggagaaaca
     1201 gtagcagatg acgctaatga tctagcccct caagctcctg ctaaaactgc tgatacacca
     1261 gcaacctcaa aagcgactat tagggatttg aacgaccctt ctcatgtcaa aaccctgcag
     1321 gaaaaagcag gcaagggagc tgggaccgtt gttgcagtga ttgatgctgg ttttgataaa
     1381 aatcatgaag cgtggcgctt aacagacaaa actaaagcac gttaccaatc aaaagaaaat
     1441 cttgaaaaag ctaaaaaaga gcacggtatt acctatggcg agtgggtcaa tgataaggtt
     1501 gcttattacc acgactatag taaagatggt aaaaacgctg ttgatcaaga acacggcaca
     1561 cacgtgtcag gaaatgctcc atctgaaatg aaagaacctt accgcctaga aggtgcgatg
     1621 cctgaggctc aattgctttt gatgcgtgtc gaaattgtaa atggactagc agactatgct
     1681 cgtaactacg ctcaagctat cagagatgct gtcaacttgg gagctaaggt gattaatatg
     1741 agctttggta atgctgcact agcttacgcc aaccttccag acgaaaccaa aaaagccttt
     1801 gactatgcca aatcaaaagg tgttagcatt gtgacctcag ctggtaatga tagtagcttt
     1861 gggggcaagc cccgtctacc tctagcagat catcctgatt atggggtggt tgggacacct
     1921 gcagcggcag attcaacatt gacagttgct tcttacagcc cagataaaca gctcactgaa
     1981 actgctacgg tcaaaacaga cgatcatcaa gataaagaaa tgcctgttat ttcaacaaac
     2041 cgttttgagc caaacaaggc ttacgactat gcttatgcta atcgtggtac gaaagaggat
     2101 gattttaagg atgtcgaagg taagattgcc cttattgaac gtggcgatat tgatttcaaa
     2161 gataagattg caaacgctaa aaaagctggt gctgtagggg tcttgatcta tgacaatcaa
     2221 gacaagggct tcccgattga attgccaaat gttgaccaga tgcctgcggc ctttatcagt
     2281 cgaagagacg gtctcttatt aaaagacaat cccccaaaaa ccattacctt caatgcgaca
     2341 cctaaggtat tgccaacagc aagtggcacc aaactaagcc gcttctcaag ctggggtctg
     2401 acagctgacg gcaatattaa gccggatatt gcagcacccg gccaagatat tttgtcatca
     2461 gtggctaaca acaagtatgc caaactttct ggaactagta tgtctgcacc attggtagcg
     2521 ggtatcatgg gactgttgca aaagcaatat gagacacagt atcctgatat gacaccatca
     2581 gagcgtcttg atttagctaa gaaagtattg atgagctcag caactgccct atatgatgaa
     2641 gatgaaaaag cttatttttc tcctcgccaa cagggagcag gagcagtcga tgctaaaaaa
     2701 gcttcagcag caacgatgta tgtaacagat aaggacaata cctcaagcaa ggttcacctg
     2761 aacaatgttt ctgataaatt tgaagtaaca gtaacagttc acaacaaatc tgataaacct
     2821 caagagttgt attaccaagt aactgttcaa acagataaag tagatggaaa acactttgcc
     2881 ttggctccta aagcattgta tgagacatca tggcaaaaaa tcacaattcc agccaatagc
     2941 agcaaacaag tcaccgttcc aatcgatgct agtcgattta gcaaggactt gcttgcccaa
     3001 atgaaaaatg gctatttctt agaaggtttt gttcgtttca aacaagatcc tacaaaagaa
     3061 gagcttatga gcattccata tattggtttc cgaggtgatt ttggcaatct gtcagcctta
     3121 gaaaaaccaa tctatgatag caaagacggt agcagctact atcatgaagc aaatagtgat
     3181 gccaaagacc aattagatgg tgatggatta cagttttacg ctctgaaaaa taactttaca
     3241 gcacttacca cagagtctaa cccatggacg attattaaag ctgtcaaaga aggggttgaa
     3301 aacatagagg atatcgaatc ttcagagatc acagaaacca tttttgcagg tacttttgca
     3361 aaacaagacg atgatagcca ctactatatc caccgtcacg ctaatggcaa accatatgct
     3421 gcgatctctc caaatgggga cggtaacaga gattatgtcc aattccaagg tactttcttg
     3481 cgtaatgcta aaaaccttgt ggctgaagtc ttggacaaag aaggaaatgt tgtttggaca
     3541 agtgaggtaa ccgagcaagt tgttaaaaac tacaacaatg acttggcaag cacacttggt
     3601 tcaacccgtt ttgaaaaaac gcgttgggac ggtaaagata aagacggcaa agttgttgct
     3661 aacggaacct acacctatcg tgttcgctac acgccgatta gctcaggtgc aaaagaacaa
     3721 cacactgatt ttgatgtgat tgtagacaat acgacacctg aagtcgcaac atcggcaaca
     3781 ttctcaacag aagatagtcg tttgacactt gcatctaaac caaaaaccag ccaaccggtt
     3841 taccgtgagc gtattgctta cacttatatg gatgaggatc tgccaacaac agagtatatt
     3901 tctccaaatg aagatggtac ctttactctt cctgaagagg ctgaaacaat ggaaggcgct
     3961 actgttccat tgaaaatgtc agactttact tatgttgttg aagatatggc tggtaacatc
     4021 acttatacac cagtgactaa gctattggag ggccactcta ataagccaga acaagacggt
     4081 tcagatcaag caccagacaa aaaaccagaa gctaaaccag aacaagacgg ttcaggtcaa
     4141 acaccagata aaaaaaaaga aactaaacca gaaaaagata gttcaggtca aacaccaggt
     4201 aaaactcctc aaaaaggtca atcttctcgt actctagaga aacgatcttc taagcgtgct
     4261 ttagctacaa aagcatcaac aagagatcag ttaccaacga ctaatgacaa ggatacaaat
     4321 cgtttacatc tccttaagtt agttatgacc actttcttct tgggattagc agctcatata
     4381 tttaaaacaa aacgccaaaa agaaactaaa aaatagtatt acttaaactg ataagagctt
     4441 atttaataga gagaaccaat ttggttctct ctatttt
//
LOCUS       DATRNX010000126         4440 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRNX010000126 DATRNX010000000
VERSION     DATRNX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            135..902
                     /gene="cfb"
                     /locus_tag="VBC81_001793"
     CDS             135..902
                     /gene="cfb"
                     /locus_tag="VBC81_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6912804.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1075..1377
                     /locus_tag="VBC81_001794"
     CDS             1075..1377
                     /locus_tag="VBC81_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6912805.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1534..2397)
                     /locus_tag="VBC81_001795"
     CDS             complement(1534..2397)
                     /locus_tag="VBC81_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6912806.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2702..3262)
                     /locus_tag="VBC81_001796"
     CDS             complement(2702..3262)
                     /locus_tag="VBC81_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6912807.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3259..3576)
                     /locus_tag="VBC81_001797"
     CDS             complement(3259..3576)
                     /locus_tag="VBC81_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6912808.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            3790..4404
                     /locus_tag="VBC81_001798"
     CDS             3790..4404
                     /locus_tag="VBC81_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6912809.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT         1514 a          679 c          778 g         1469 t
ORIGIN      
        1 tgtaataatg taaaatatag gggaaaagaa agcgcttgac gaccttttgg acaagtagta
       61 agatacaaac atgggccctg taaattaaaa atactgcagt aaaagtgatt ttagtttaaa
      121 ggaggaaatt tattatgaac gttaaacata tgatgtatct atctggaact ctagtggctg
      181 gtgcattgtt attttcacca gctgtattag aagtacatgc tgatcaagtg acaactccac
      241 aagtggtaaa tcatgtaaat agtaataatc aagcccagca aatggctcaa aagcttgatc
      301 aagatagcat tcagttgaga aatatcaaag ataatgttca gggaacagat tatgaaaaac
      361 cggttaatga ggctattact agcgttgaaa aattaaagac ttcattgcgt gccaaccctg
      421 agacagttta tgatttgaat tctattggta gtcgtgtaga agccttaaca gatgtgattg
      481 aagcaatcac tttttcaact caacatttaa caaataaggt tagtcaagca aatattgata
      541 tgggatttgg gataactaag ctagttattc gcattttaga tccatttgct tcagttgatt
      601 caattaaagc tcaagttaac gatgtaaagg cattagaaca aaaagtttta acttatcctg
      661 atttaaaacc aactgataga gctaccatct atacaaaatc aaaacttgat aaggaaatct
      721 ggaatacacg ctttactaga gataaaaaag tacttaacgt caaagaattt aaagtttaca
      781 atactttaaa taaagcaatc acacatgctg ttggagttca gttgaatcca aatgttacgg
      841 tacaacaagt tgatcaagag attgtaacat tacaagcagc acttcaaaca gcattaaaat
      901 aatatttgta tttttcgtgt gatgctgtcg acttcgtgat tttgtactac catgattgtt
      961 atgattaaaa gatttacgac aatagtcata atagtagaac gatgtcacca ttttaaataa
     1021 taaagtgatt agtcatttga ctaaatttgc caagtatcaa aggaaataaa gattatgact
     1081 aaaaagataa ctgttgtagc attagaaaca ttgattgccc agcataataa tatccatttg
     1141 atagacgttc gtgaagagca tgagtatcgt ggagggcata ttccaggtgc gataaatctt
     1201 cctttgagtc aactcagtca taagtttgaa cagttagata aaaataagga atattatctt
     1261 gtttgtcaac gagggggaag atctattaga gcatgccaat ttctcgagtt gaaaggctac
     1321 aaagtaatca atgtagatgg tggcacagaa gcgtggagag gaaatctaga agtgtaaata
     1381 tgtcctgtgt agtctaccat atttgataat aaaaaaattg acattatctc actattaatg
     1441 accttttagt aaacataaag aaaagtatgt gttttgaaaa ggaggatgat tatccttttc
     1501 aaaaccatac tttttgtgtc ttgtggcttg gtcttaatta atagttgtta ggacaatgtc
     1561 gtaaatgaca aggagaataa aaggccaact agtaagtaat aattttgtgt gttgtttaga
     1621 gtttattgag tcagcctctc gaacgttaga gtgcagactt atacgacggc gagcagtatt
     1681 cctatgcgta aaaaggtaat ataatataaa taagattgct aaaattagca atatattttc
     1741 aaaataagtc ccgtataact cgtttactgt tgatagctgg gtgaaaattt ctacgataaa
     1801 gttattgcag atatgaagga gtagggctac cttaaaggaa taatggtaag cgataaaacc
     1861 cattatgata cccgcaatag atgtagtcaa tagctgagaa atgtcttggt gcattaaacc
     1921 aaataggatg ccagaagtga taatggcaaa ccatttacca tacttggata ataaattcaa
     1981 tactattcct cggtataaaa cttcttcaaa aaaaggacct acaacaacag aataactaat
     2041 cataagtata ctgaatgtgg catcataatt ttgactttcc gattccataa tattgagatg
     2101 atttagcaca gcatcccata ttgtggagac tatattatta gtagtatata taatgcttaa
     2161 caagaataaa atagtggtga aagtgattgt atgatgagtt gccgttagat gtttttttaa
     2221 atttttaaaa cccataaaaa aacaaggaat gagtaaggcg attaaagtac ttgctatacc
     2281 tatccagaca tcattttgta agatttcagc aaatagtgta gtcgccaaac ttaaaacaat
     2341 agcctgagct aaaagtatca gcccgattag gttaagtctc tttttttctt gattcaattg
     2401 attacctcct tattaataac ggtaacgaca aagaaatatt ataatgtata atgtataggt
     2461 ttctaagtca ttagtcaata gggacgagca cttggatact tctaataaga taaaatacat
     2521 agacaagact atggttttgg cttctctaaa gattacctaa aaatttataa gttctcgagc
     2581 agaaaggatg agtttggata ttgagaggat gggcttgacc agctgaaaga aaaatgaacc
     2641 aagggtaatc gcttcgaaaa aagaaggaaa tttcaattag aaacttcctt tttttatcaa
     2701 tctagtattg gcgcgtgcta gggactttat aattcgttat aaaatcttcg atttcagcaa
     2761 tatcatctga gaataggaca ttttctctat cttccagact aagaaaaccc tcactaacca
     2821 tatgatcaaa catatttttt aaatcattaa agtagccatt aacattatat aaaatgcaag
     2881 gattatcatt ttgaccgatg cgagaccaag agataacttc ggaaatttct tcaagggtac
     2941 caggtccacc aggtagagcg ataaaggcat ctccaagtag catcattttt gccttacgtt
     3001 caggcatatt atttactatg ataagctctg ataaattttc atgtgcaatt tcacgatctc
     3061 ttaaaaatgt cggaataaca ccagtagtat agccaccatt tgcaatcaca gtatcagcca
     3121 tgactcccat taagccaact tttcctccac catacaccag agcatgtttg ttttgtgcca
     3181 tccattgtgc caaggcaact gttttttcac tataaacagg attttcccca gtacttgctc
     3241 cacaaaaaat agttattctc atgtttcaaa cctcttttct aatttagtgc gatgtgcgct
     3301 gataatctct tctagtgaga tattatactg atcagcaagt atgaagatat tatctaagac
     3361 atctcctagt tcttctttta tatcatttaa gttttccaaa tcagtttgtt cggtttcgtc
     3421 aggtctgtca cgtccaattt catatttgcg aatggcttga gccaattcgc cgacttcttc
     3481 tgaaagaaag ttacttctaa taaaagaatt gtattgatac cagtttcttt ttttatagaa
     3541 ttcgctcacc catttttgat aatctgtaat atccattgtt ccctccgttt caagtatatt
     3601 gtagcaatat tgctttatta atgaaaattt gaacgctaat taataggaaa agttcgtatt
     3661 tggtatttct tctagtttgt gaatagtcat atttaaactt aagaggttag cgcgtgcttt
     3721 aagcattttt taaatatttt aaaccataat gtatgaagat ttatctcttt attaaagtga
     3781 ggttatgtag tgaacaagaa atcctttttc cagtgtgtta tactacttgt ttctttttta
     3841 gtgttaacat tagcagttaa atctcaaagt gatatgataa gttatttgga taatatcact
     3901 tcagcttttt ttcaaagcat acgaaactcc gatttaacta acttaatgac gataatatca
     3961 acagttgtca gccctttgac aacgtcatta atagcacttg ttatccttgg gtatcaatat
     4021 tttttaaacc aaaggatagc tgtgtggcta tttatgttat tttttggtac caatgctttg
     4081 gcattgcttt taaaagatat catcgcacgt catagaccta tgaatcaact tatttttgac
     4141 tcaggttata gttttcccag tggacatacg atatcaacgt ttttattaat gattcttgtt
     4201 ttagtggttg caagacagag attgagaaga gtattgtcac aagtagtttt cgtaatattt
     4261 gcattagtaa tccttgcatc agttattttt tcaagacttt atttagaaaa tcatttttta
     4321 acggatattt tagggtcgtt gttactagga gcatcgtcat attgtggttt gtctgctatt
     4381 gtatctttga aagagttaca ataaaaatag tagttaccaa tgaattataa aagagccttt
//
LOCUS       DATRNX010000127         4340 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRNX010000127 DATRNX010000000
VERSION     DATRNX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            73..924
                     /locus_tag="VBC81_001799"
     CDS             73..924
                     /locus_tag="VBC81_001799"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO6912810.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPICAGLGGG
                     STDAAATIRALNRLWNLQMGHDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            1011..1454
                     /locus_tag="VBC81_001800"
     CDS             1011..1454
                     /locus_tag="VBC81_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6912811.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            1457..2167
                     /locus_tag="VBC81_001801"
     CDS             1457..2167
                     /locus_tag="VBC81_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6912812.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            2157..2969
                     /locus_tag="VBC81_001802"
     CDS             2157..2969
                     /locus_tag="VBC81_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6912813.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(3180..4210)
                     /locus_tag="VBC81_001803"
                     /pseudo
     CDS             complement(3180..4210)
                     /locus_tag="VBC81_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         1264 a          633 c          849 g         1594 t
ORIGIN      
        1 catcttaatt gtccgaattt gtcatttgtt atgttataat atggatattc tatattttat
       61 tagggaggtt ttatgaaaat ttttgagaaa gctcctgcca aacttaattt aggattagat
      121 attaaaggac gatgtgacga tggttatcat gaattagcta tgattatggt tagtattgat
      181 cttaatgact acgttaccat ttctgagcta aaggaagatt gtattgttat cgattctgat
      241 agtagcaaaa tgcctttaaa taatgataat gatgtgttta aagctgcaga tataatcaaa
      301 aatcaatatg gtattaataa aggtgttcat attagattag aaaaatccat tcctatctgt
      361 gcaggactgg ggggggggtc tactgatgct gctgctacaa taagagctct taatcgatta
      421 tggaatcttc aaatgggcca tgacgaaatg gttgctattg gttttaaaat tggtagtgat
      481 gttccttatt gccttggtgg gggatgttcg ttagtattag gtaaaggtga aattgttaaa
      541 cccttaccga cattgagacc atgttggata gttctggtta agccggattt tggtatttca
      601 actaaatcca tttttagaga tattgattgt aagtctataa gcagagttga tattgattta
      661 ttaaagtctg ctattttgtc atcagattat cagttaatgg tcaaatctat gggaaattca
      721 ctcgaggata ttacaattac taaaaatcca gttataagta caataaaaga acgtatgtta
      781 aattctggag ccgatgttgc attaatgaca ggaagtggac caactgtttt ttcaatgtgt
      841 tctacagaga aaaaagctga tcgtgttttt aatagtatga aaggtttttg caaagaagtt
      901 tataaagtta ggttgttgag ataaaaggtt ttatcttctt cacctatcta tgattgattc
      961 ttctatatct tcgtgatata atttactggt taacaaaaaa agaggtatat atgacagttt
     1021 tagaacaaaa attagaccat ttagtgagtc aaatcttgct taaggcagaa aatcaacatg
     1081 aattattgtt tggtacttgt caaagtgatg taaaattgac taatacgcaa gaacacattc
     1141 tgatgctttt atcacaagag cagttgacaa attctgattt agctaagaag ttaaatatta
     1201 gtcaggcagc tgtaactaaa gcagtaaaaa gtttaatttc tcaggatatg ttgaaagcga
     1261 ataaagattc taaagatgct cgtattactt attttgagtt atctgaactt gctaaaccca
     1321 ttgcagatga acacacacat catcatgaca atacgttagg ggtttacgga agattagtaa
     1381 atcatttttc aaaggacgaa aaggtcgttt tagaacgttt tcttgatctt ttctctagag
     1441 aattagaagg gtaatcatgc gatatattac agttagtggt cttacttttc agtatgatag
     1501 cgatcctgtg ctggaagggg ttaattacca tttagacagt ggggagtttg ttaccttaac
     1561 aggtgaaaat ggggctgcta aatcaacttt aataaaggct actttaggta ttttaacgcc
     1621 caaagtgggt acagttaata tctctaaaga aaacaaagaa ggaaaaaaat tacgtattgc
     1681 ctatttacct caacagattg caagttttaa cgctggcttt ccatcgtcag tttatgaatt
     1741 tgtgaaatct ggacgatacc ctagaaatgg ttggtttaga agactgacta aacatgatga
     1801 agaacatatt agggttagtt tagaagctgt tggtatgtgg gataatcgcc ataaaaagat
     1861 aggaagttta tctggcggtc aaaagcagag ggctgtcatt gctaggatgt ttgcttctga
     1921 tccagatatt tttgtattgg atgagccaac aactggtatg gacgcaggga ctactgagaa
     1981 attttatgag ttaatgcatc ataatgctca taagcatggt aagtctgttt tgatgattac
     2041 tcacgatcct gatgaagtaa aggggtatgc tgaccgaaat attcatcttg ttaggaacca
     2101 atctttacca tggcgttgtt tcaatgtcca tacaaacgaa atggaggttg aatcagatgc
     2161 tacttgatat gctttcctat gattttatgc aacgagcgct cttagcagtt gtggctatta
     2221 gtatttttgc tccaatttta ggcatcttcc taattttacg tcgtcagagt ttgatgagtg
     2281 atactttgag ccacgtttcc ttagccggtg ttgcattagg cgttgtttta ggtatctcgc
     2341 ctacgtggtc aactattttc gttgttactt tggcggcagt tgtattagag tatttgcgta
     2401 ctgtatacaa acattatatg gaaatatcaa ctgctatcct gatgtcgatg gggttggcta
     2461 tatctttaat cgttatgagt aaagctcata atgttggtaa tgtcagtctt gaacagtatt
     2521 tatttggttc tattatcact attgggaagg aacaagtgat tgctttgttt gttattgcat
     2581 taatcacttt tattttaacc atcctattta ttagaccaat gtatatatta atgtttgatg
     2641 aggatacagc ttttgtagat ggattgcctg tgcgtacaat gtctattttg ttcaatgttg
     2701 ttacaggtat tgctattgcg ttaacaattc ctgccgcagg tgctctattg gtgtcaacta
     2761 ttatggtttt accagctagt attgctatgc ggttaggtcg aaattttaaa actgttattt
     2821 ttcttgggat gttaattgga tttgtgggaa tggtagctgg tattttcctt tcttattatt
     2881 gggagacacc tgctagtgca acaataacga tgatttttat tggcattttc ctattagtta
     2941 gcttagtagg cttgcttcgc aaacgttaaa taaaaaaatc tatcttattc taggatagat
     3001 tttttattta tctccctgtt tttttcgaat atattttatg agggatcagt tgagaaatta
     3061 tctcgatgag gtatctacta agttttctgg cttaagttga tagaaggggc aatgtgaatc
     3121 tatttttgcg ttttaggata aaaaatacta ccggcaattt tgccggcagt aaattgtaat
     3181 tattgtgctt cagtagtgct gtgatatttg tgttgattta acgcttcttg ttcagtcatt
     3241 tgaacgatat tagcggtatt tgcatttttt atgttttcta aactatacca ataagtattt
     3301 gaacgaccct tatttgcaac gtagacgatt ctatttttgg gagctacatt atttgtatct
     3361 gattcagaag tattagtgtt agcattttct tctatatcag atgcaccttc aatagctgct
     3421 tcgttttcat cagcatcgct atttggatct tcaccttctt ctgcctgttt cccatcttct
     3481 ttgattagca taccagtttt gtaatcaatt tttgcgagtg gagacgtatt ttctaatgta
     3541 acagttgtga cgccgtattc atcaacttgt tctttgccgc cgcctaattt gatttctaga
     3601 agttttcctt gtttatcaat tccaacatac tgaagttcaa ctcggctagg tactaaatta
     3661 tttccttcgt atattggagt cactttgtaa tcaagctaga aatttttatg atttgccaac
     3721 caactatcta atctattttc gtagtataac ataccatcag gattattttc gctaaatcct
     3781 gtatttagat atttggtcat agtaactagg ttctcaggaa cattgttcat tcctgaaaat
     3841 tgataaccaa cgaggtgacc tcggtctata agccaagttg ttttaccctt ttcagtagta
     3901 aatttgtagt tgtgccaacc ggttggatta tacgtcagtc tttctctttt tatcttaggc
     3961 tcatctgcat cccttaattg tatgtgagaa tatgttgctc tttctaagtt atctaatgaa
     4021 cctaccttga tttgcttatt atgagtaaac ggtaagattt ttttgaagaa gtacttacat
     4081 actgtgtctc aacatgggat gtcgtcttat aatgtttagc cttagcttca acattctgtt
     4141 gtaatgcccc aacgcatagt acgacagtta atagtgggag tgatttgtat aattgtttag
     4201 ataatttcat aaacctaaac ccttctaaat gatatttgtg aaggcctttc acaagtataa
     4261 ttttatcatt ttattgggaa aaagtaataa aaaaacataa ctagttaatt tttttggaga
     4321 ttatgttatt taactagata
//
LOCUS       DATRNX010000128         4256 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRNX010000128 DATRNX010000000
VERSION     DATRNX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            147..1355
                     /locus_tag="VBC81_001804"
     CDS             147..1355
                     /locus_tag="VBC81_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6912814.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYSNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            1549..2044
                     /locus_tag="VBC81_001805"
                     /pseudo
     CDS             1549..2044
                     /locus_tag="VBC81_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(2019..2274)
                     /gene="mobC"
                     /locus_tag="VBC81_001806"
                     /pseudo
     CDS             complement(2019..2274)
                     /gene="mobC"
                     /locus_tag="VBC81_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2284..2421)
                     /locus_tag="VBC81_001807"
     CDS             complement(2284..2421)
                     /locus_tag="VBC81_001807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912815.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2554..3698
                     /locus_tag="VBC81_001808"
     CDS             join(2554..2788,2788..3698)
                     /locus_tag="VBC81_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO6912816.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEENYFNAIK"
     gene            complement(3819..4151)
                     /locus_tag="VBC81_001809"
     CDS             complement(3819..4151)
                     /locus_tag="VBC81_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6912817.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
BASE COUNT         1386 a          848 c          683 g         1339 t
ORIGIN      
        1 aatggtgaga gatgatgaag agagagtttg ctaattagcg tgtcttttta gtgtatccac
       61 aagatagttg ttttcatcat tttattttgt agtctttaca aacctaaaga aaaattctag
      121 aaatgataat gaaggcggtc tatcaaatgg ttacattcga ttttaaacat gttcaaacat
      181 tgcataccat atctcaacta cctatttcag tcatgtcacg agacaaggaa ctcgttcagt
      241 tatatagtaa cgaagactat ctgttgcctt actatcagtt tttaaaacat ttagctatcc
      301 cttataacca agatatcact gtttatgagg gtctttttga agaatcattc ttaatttttc
      361 ctgtctgtca atatcttatt gctattggac cattttatcc ctatagccct gatatcaata
      421 ggcaagaaca gttatccagt cgctttctag aacaattttc tcatcgtagt aaaaaagaaa
      481 tcttatccta tataaagctt gtcccttgtt ttcctactac cagtatacgt agtcttcttg
      541 tgtctatcga tgcctttttc caaacacagt ttgaggctag ttgccaacaa gtcatcaatc
      601 atttattaga agagtcagaa cagattgttg cggatcctga tattgttctt cacctaaaac
      661 atactaagaa aaactctttt cagttaccca ctgttttaaa ccatctcaat cacattattg
      721 atctcgttaa gctgggtaac actcaactgt taaagcaaga aattaatcgc ctcccatcat
      781 ccagtgttac ctcatcttca atccctgccc taagagctga aaagaactta actgttgttt
      841 acttaacaaa attactagaa ttaagtttcg aggaaaatac tgatgtggct aaaagttatg
      901 cgctggtaaa gcactatatg gctttaaacg aagaggctcc tgatcttatt gatgtttcaa
      961 gaattcgctg tgcagctctt attgattttt cagaatcctt aaccaataag agcatctctg
     1021 acaagcagca aatgtacaat agtattctcc attatgtgga caaccacctc tactccaaac
     1081 tcaaagtatc tgatattgcc aactacctat atatctcaga ttcccactta cgctcagttt
     1141 ttaaaaaata ctctgacatt tccttgcaaa gttatattct aaaggcaaaa attaaggagg
     1201 gacaattact actgcaaaga ggggtaccga ttggggaagt agcgaaatta ttacattttt
     1261 acgacaccac acattttctt aaaaccttta aaaaatacgt gggaatatct tcaaacgaat
     1321 atcttactaa atatcgtgag acctcatgcc agtaacatag caatccatga aggtacggaa
     1381 ttatttcatc gtccttttgc cttcatctga aacgactaaa aacagccttc tcgtttaaac
     1441 ggaagactct tttgttacct ttcaaaatcc actggcacta ctttttagtc taaaattttc
     1501 agctaatcca gtccatacaa actctctctt tgtgctataa cataggttat ggataaaatt
     1561 attaaatcaa tttctgcctc tggtgccttc aggtcttatg tcctcgatag caccgagact
     1621 gttaaattgg ctcaggaaaa acatcatacc ttgtcgtctt caacagttgc tcttggtcgc
     1681 accctgattg ctaatcaaat attagcggct aatcaaaaag gcgatagcaa gattacggtt
     1741 aaagtcatcg gtgattcttc ctttggtcat attatctctg ttgcagatac caaagggcat
     1801 gttaaggggt acatccaaaa tactggtgtt gacatcaaaa aacggccaca ggtgaagttt
     1861 tggttggctc ttttatgggg aatggtcatt ttgtgaccat catcgactat ggcacaggta
     1921 atccctatac ctcaacaacg cctcttatta ctgggaaaat tggtgaggat tttgcttatt
     1981 acttaaccga atcagagcaa ccccccctgc tgttgggctt aacgtgcttc tcgatgaaag
     2041 tgatactgag cctgagaaat tagatggctt tggttgatag cacgcgcaat ttgattgatg
     2101 ttatttccaa tccgtgttaa tcgtgacaaa tgacatattg ggattaagaa gaactcttct
     2161 agcatagact gagaaatttt gacaattgct cttagcaatc cgctcattta attgattcaa
     2221 ttctgaatct gttagaaaga cttttttgag attggtacga taacgataaa caattgcagc
     2281 ctcctaaccc atatactttt cacgaaactc acgactgagt ggctgtgttt gattgacttc
     2341 cacaatcaat tcttgaacgc aggtcaataa gattgacacg tgttcatgag tcacttgatg
     2401 attctctctt gcgataatca aaacttcctg cacatctcaa ctaatctgct caaacttttg
     2461 gtattgccaa agggtggatg tatactataa aagtagacaa aactttgtgt gttacaatct
     2521 gttttaaaag acacaaaaac gaaaggacaa cctatgtcaa catttaaacg ctatgatgaa
     2581 gaattcaaac aatctcttgt taacctttat caaactggaa aaactcagtc tgaactctgt
     2641 aaagactata gggtatccac ttctgcgctt gcaaaatgga tcaaacagta ctctcaattc
     2701 aaactcgaag ataattctgt acttactgcc aagcaaatac aagagttaca aaaacgtaat
     2761 gcgcagcttg aagaggagaa ccttatccta aaaaaagcaa gtgccatatt catgcaaaac
     2821 ttaaagtaag actcctcgct gtctatcggt tacgctttga acacgccaca acaaccttgt
     2881 gtcgtgtttt acgtgtcaat cgctccactt actataaatt tctaaaacat aagccctcaa
     2941 aaagagaatt ggataatcaa atttataaaa aacaaatact tgagatttat accaaagcaa
     3001 acaaaagact tggtgtgaag tctatcaaag tcattcttca aagagactac gacacaaaaa
     3061 tctctgaagg aagaatttac catctgatga agaatatggc gctccctaaa atggctaccg
     3121 ttaagccaaa aacagctctt aaaaagactc aaaaaacgta tcctcaaaac ttactcaacc
     3181 agaaatttta tcctgacaaa cctaatcaag tatggtctac tgacttcacc tatatttcta
     3241 ttggatataa gaaatatgtc tatctctgcg caatacttga tctctattct agaaaatgta
     3301 ttgcttggaa attgagtcac cgtatggatg caaagttagc atgtaacact ctagaattag
     3361 ctcttaataa aagaaagatt gaaggaacac ttctctttca ttccgaccaa gggtcacaat
     3421 ttaaggccag ggaatttaga aaaataattg atgacaacaa tatcatgcat tctttttcta
     3481 aacctggata tccttatgat aatgccgtaa ctgaagcatt tttcaagtat ttaaagcata
     3541 gacaaatcaa ccgaaaacat tatcaaaata tcaaacaggt tcaattagac tgctttgaat
     3601 acattgagaa tttttataac aattacaacc cacatacggc taatctagga ctaaccccta
     3661 atcagaaaga agaaaattat tttaacgcaa taaaataaca cggttttctg tctacttatt
     3721 tgacattagt ccactctgtg aattcagatg tggaagaaga agtaactcgc aatgttttat
     3781 ttgcttcttt ttcgtgtgtc actggaaccg ctgcatcttc agcattctaa tcctacagca
     3841 tgctccgctg attctgtagg attagaatga gatgcatctg ttgataacac tgctgaagtt
     3901 gacgtatctg tcgcaagact tgttacatct gaagtaggct gtgtaacagt aacttccggt
     3961 tttacaccag ctgtttgtgg tacctcagta ctcataatgc ttgactcatc cgccaaaaca
     4021 gaatgactac tcattcctag aaatgtcaat ccaattaata ccgatgctac accaaatttg
     4081 aatttcttaa tcgaaaaacg ctgtttcgta tgtgcgacat tataatttct ttgcttatac
     4141 ttcctaagca taacttctct ccttttcaaa agtaaaaata gattaaccaa taaagttatc
     4201 tatttttact ttttttaaag ttttactgtt ttacattatc tcgataaatg tgaact
//
LOCUS       DATRNX010000129         4251 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRNX010000129 DATRNX010000000
VERSION     DATRNX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(368..820)
                     /locus_tag="VBC81_001810"
     CDS             complement(368..820)
                     /locus_tag="VBC81_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6912818.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(832..1899)
                     /locus_tag="VBC81_001811"
     CDS             complement(832..1899)
                     /locus_tag="VBC81_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6912819.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            complement(1999..2715)
                     /locus_tag="VBC81_001812"
     CDS             complement(1999..2715)
                     /locus_tag="VBC81_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6912820.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2717..4216)
                     /locus_tag="VBC81_001813"
     CDS             complement(2717..4216)
                     /locus_tag="VBC81_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6912821.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
BASE COUNT         1389 a          872 c          671 g         1319 t
ORIGIN      
        1 atcatacacc caatcaaatg ccatattagc ataaactgga tttacatcta ttgaggtcag
       61 caacaatatc ttttctcttt tttgtaaatt atttttgcaa ttcgagattc ccagcagtac
      121 taattttaga tgttgataat agatggatca gctattctat tatcaacatc taaaactttc
      181 tgtgaaaaat cattaggaat aatgactatt aactggtgat cacctttttt aggccatttt
      241 cagtaatccc atgactaaca attgaccaat tttgagaatt atctcgttca atatatttaa
      301 cataactggc ccttagctta aacgataaaa ccttcaagga agataggcat atactctatc
      361 ttcctcttta ctcttccatc cctagtgtta cctctggaac atcatgttga acataagcaa
      421 ctccttttgc ttccataagt tcaatagcaa atgggtgtgg gcgataatta gctttataag
      481 taattttctt aaccccggct tgcaagagcg ccttggtgca attgatacaa ggaaaatgcg
      541 tcacataaat ttctgtatta ttagtagaaa taccttcttt agcacactga ataagggcat
      601 tcatctcagc atgaacagtc ctaatacagt gaccatcttc catataatgt cccacttcgt
      661 tacagttatc tgtctcagag actcctccgt tataacctgt agcaatgatg cgattatttt
      721 tgactaaaac agctccaaca aaagcacggt cacaggtaga acgtttagaa atcaactctg
      781 catttgccat aaaataatct tcccaagaaa gtctattcat tctattttcc tttaaagtac
      841 aattaattct ttcggtgcag atgtcaatac ttcgcaacct gtttccgtta ttaataaatc
      901 atcttcaata cgaacaccgt atttattatc gagatatatt cctggctcgt cagtcactac
      961 catcccagat gcaatcactc cctctgactt accaaaataa ggaatttcat gaacatcaag
     1021 ccccatacca tgccctatac cgtgggtaaa atgattaccg tatccagaat tttcaatgac
     1081 ttgtcgcgcc aagtaatcat aatcacaacg cttcattcct gattttacat tatcaataat
     1141 agcttgatta cttttcaaaa cgatatcgta aatctcgcgt tcctgatcag tgacatgacc
     1201 gatatgaatc gtacgtgtca tatcactaac atagtgctga tagtaacaac caaaatctaa
     1261 agtcagagtt tctcccgatt gaatagtctt ctgactcgca accccatgtg gcatcgctga
     1321 tcgatatcca gatgctacaa taaaatcaaa tgaaggacca gttgctccta attctctcat
     1381 gcgaaagtct aaaaaattag caacttgcaa ttcagttgtt cgatcaggct ttataaaatc
     1441 caacacatct aaaaaagctt ggtccgaaat acgacaggct tgacggatga tcgcaagttc
     1501 tgttacatct ttaatcatac gtaacttttc aacaacagat ggagcttcta tcaagcgata
     1561 atcttcaaaa acttccacca tatgtttgta catatgataa gaaatatcag tttcaaaacc
     1621 aatcgctatc aatgcgtcat cgcgaataat tttcaaaata gccgcaagtg gttctctgct
     1681 aatgataata tcaaactctc ttacagtatt ctttgctatc aaggcatatc gtgaatctgt
     1741 aacaaaaatc cggtgttttg gactaatgag taccgttgca ttagttcctg aaaaaccagt
     1801 caaataaaaa atatttgtta aatttgttat taagagtgcg tcacatgata attgtgaaat
     1861 tgctgtttga catctagtta gacgttgttc aaaacccata agaaacctcg ctattctaac
     1921 cttaattagg ttaattttat catcttttaa aacatttagc tataagaaaa gcaactcatt
     1981 tcagttgctt ttactttact attcttttat attaagagct tcttttaatg ctttaacttg
     2041 catctcagca taagcttctg gatttgtctt agctaatgtc tttatctcca tagctttcac
     2101 tgggtttact tttgcttgcg aagtcatttg ttcttttagt ttagccgcag ccgattctaa
     2161 gtgttcggac tgctctttag tcaaaactag ccaagttttt cgtggtattt tagcaactga
     2221 tttctgagaa atcaattctc cattttcacc tgcaaagcca taaagtagtt tcgaaagact
     2281 atctttccac acataacgtt tagtctttgt tgttactgtg gcttttttca catcagctaa
     2341 ttggtagcta gcagattttt tcacagcact tgtcacttct tttgtatttg gaacaaagtg
     2401 agcttttgct tctttttgat ttaaatctgt tcgataaact aaaacattat tactcgaatg
     2461 agtaccaatc tctttataaa tcatgagacc aaatggagcc ttactatcac ccgcactata
     2521 aatctcacgt gtctttgtct tagtttcaat agtcatacct gtatgattaa taaagtgatc
     2581 cgttaacatc caaactgagg caaacaacaa agctaatgag agaagaccta gaatataccg
     2641 tagagctttt ggacgaatca acatccatgt gacaaatgtc aaaatagttg ccagtgcaat
     2701 aatcaaaata atcatgctat acctcctttt ccgtctcaat aattttccct tttctcagga
     2761 aaagagatac cagtaaacca agaaccgcaa atacaaaacc aatggcaaaa gaaacatgga
     2821 agccatcaag actagcattc aacatctgat ttgcatattt taaaggattc attgttagca
     2881 aatcttttga tggtttattg ttggtgataa tattttgagc aacacttgaa agcaatgcaa
     2941 ctacaactgc tgaagcaatt tgacgagcag tattattagc tgctgttcca tgtgcggcct
     3001 catgtggtgg aagtgcgctc atcgcacttg ctgtcaatgg catcataacc atagcaatac
     3061 caaacatacg tactgcatat aagagggtaa taaaatgatc tggtgttgta gtcgttaaga
     3121 aaacaaaagg taacgtcgct acacctagta tggtaaaacc aatcatagcc atacgtcttg
     3181 caccaacttt atcatataca gctccagaaa ttggactaac tatccccatc atcaaggcac
     3241 ctggtaataa agctaaacca gaatcaagtg cagagagacc atgaacattt tgcaagtaaa
     3301 gcggtagcat catttcaaca cccatcatcg ccatcattga aagtgcaata gctgctgttg
     3361 ttactgagaa ttgtttattt ttgaaaacac ggatatctaa gaacggtgtt tctagtttta
     3421 attgacgatg aatgaataag gcgataaaaa taataccaac cataatagga gaaataacat
     3481 atccaatatc tccccatcca taagttgcca catttgtgaa gccccacaaa aagctaccaa
     3541 agccaattat tgaaaggata agggaaggca cttccaattt aactgactta ttatcaacca
     3601 catcttttag aacaaagggg gataaaattg tagtgaccgt taaaattagt aaaggaagaa
     3661 ggaaaattgc acgccatgtt aaatcatgcc ctgcaaaatg aaattcttgt tttaaaatcc
     3721 accctgctaa tgtaggccca atagctgggg caagaccaac caccaaacca ttaagtccca
     3781 tagcggcacc acgctgctct ggaggaaaaa cattcaccat aacaacctgc atcaaaggca
     3841 tagaaatccc tacagaaatc gcttgaataa tacgtcctac taaaaaaagg ttccagttag
     3901 atgtaggtgc taacgtcgtc attaacagtc caataagtaa aacaacgtag gatgtcacat
     3961 acaaccattt tgttgaaaaa cgtgtcgcta aataagctga aacaggaatc ataatcccat
     4021 tcgctaataa aaaccatgtt gttgcttgtt gagccgttga caacgaaatg ttaaagctat
     4081 tcatcaaagt tgggatagcc gttcccaaac ttgtctgatt taatacccct gcaaatgtag
     4141 caatcaacaa taaggtgatc atggctgtac gattatatgg tttgccatga atatcatgtt
     4201 ttgcttttgt agtcatttta tctccaatct ctatttctga ttttaactaa t
//
LOCUS       DATRNX010000130         4239 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRNX010000130 DATRNX010000000
VERSION     DATRNX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..864
                     /gene="dnaE"
                     /locus_tag="VBC81_001814"
     CDS             <1..864
                     /gene="dnaE"
                     /locus_tag="VBC81_001814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6912822.1"
                     /translation="IYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGF
                     ISPLIAIGAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYE
                     MEQDILGVGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQ
                     MAFLTVMDINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEA
                     TEERFWILLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALD
                     LSEFVVKTVYQ"
     gene            945..1967
                     /gene="pfkA"
                     /locus_tag="VBC81_001815"
     CDS             945..1967
                     /gene="pfkA"
                     /locus_tag="VBC81_001815"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6912823.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            2016..3518
                     /gene="pyk"
                     /locus_tag="VBC81_001816"
     CDS             2016..3518
                     /gene="pyk"
                     /locus_tag="VBC81_001816"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6912824.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            3689..>4239
                     /gene="lepB"
                     /locus_tag="VBC81_001817"
     CDS             3689..>4239
                     /gene="lepB"
                     /locus_tag="VBC81_001817"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6912825.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTAS"
BASE COUNT         1348 a          647 c          900 g         1344 t
ORIGIN      
        1 atttatttag gtttaaaaaa tattaaggct gttcctaacg acttggctta ttggatttct
       61 aaaaatcaac catttcagag tattgaagat tttctaatga gactgccaca gcagtttcaa
      121 aaaagtggtt ttatttctcc gctaattgct attggagcat ttgacgaatt tgataacaat
      181 agacggaaaa tcacctctaa tcttgattct ttatttacat ttgtaaatga actaggtagt
      241 ttatttgcag atacctcata tcattggtta gaagtagaag atttctctaa ttctgagaaa
      301 tatgaaatgg aacaagatat cttaggagta ggaatcagtc cgcacccttt agtggggatt
      361 tcacaaaaag ctagccgtcc gtttatacca attagtcaag tccaagaaaa tagcgaagct
      421 actattttag ttcagctaaa gcaggtcaaa atcattagga caaaaagttc tggacaacaa
      481 atggctttct tgactgttat ggatattaat tcaaaaatgg atattactgt ttttcctgaa
      541 actttcaata ttgtaagaga tgatttacaa gaagggaaat attattactt gcatggaaaa
      601 attcagaagc gagatgagcg attacagatg gttttaaacg gtgttcaaga agcaacggag
      661 gaacgttttt ggatacttct aaaaaatcat gataacgaca aaaaaatatc ggaaatattg
      721 tccaaatata aagggcatat tcctgtctat ttgcattatg aaacaactaa agaaactatc
      781 caaagcaaag tacatttggt tagaaaagat agtggcttag cccttgattt atctgaattt
      841 gttgtgaaaa cggtttatca ataaaattgc atataaagtg cagaaattta caaattaata
      901 tgctataatt aaatcgttaa attaataaaa ggagtaccaa ttaaatgaaa cgtattgctg
      961 ttttaactag tggtggtgac gcccctggta tgaacgctgc tatccgtgca gttgttcgta
     1021 aagcaatttc tgaaggtatg gaagtttacg gcatcaacca aggttactat ggtatggtga
     1081 caggggatat tttccctttg gatgctaatt ctgttgggga tactatcaac cgtggaggaa
     1141 cgtttttacg ttcagcccgt tatcctgaat ttgctgaact tgaaggtcag cttaaaggga
     1201 ttgaacagct taaaaaacac ggtattgaag gtgtagtagt tatcggtggt gatggttctt
     1261 atcatggtgc tatgcgtcta actgagcacg gtttcccagc tgttggtttg ccgggtacaa
     1321 ttgataacga tatcgttggc actgactata ctattggttt tgacacagca gttgcgacag
     1381 cagttgagaa tcttgaccgt cttcgtgata catcagcaag tcataaccgt acttttgttg
     1441 ttgaggttat gggaagaaat gcaggagata tcgctctttg gtcaggtatc gctgcaggtg
     1501 cagatcaaat tattgttcct gaagaagagt tcaatattga tgaagttgtc tcaaatgtta
     1561 gagctggcta tgcagctggt aaacatcacc aaatcatcgt tcttgcagaa ggtgttatga
     1621 gtggtgatga gtttgcaaaa acaatgaaag cagcaggaga cgatagcgat cttcgtgtga
     1681 cgaatttagg acatctgctc cgtggtggta gtccgacggc tcgtgatcgt gtcttagcat
     1741 ctcgtatggg agcgtacgct gttcaattgt tgaaagaagg tcgtggtggt ttagccgttg
     1801 gtgtccacaa cgaagaaatg gttgaaagtc caattttagg tttagcagaa gaaggtgctt
     1861 tgttcagctt gactgatgaa ggaaaaatcg ttgttaataa tccgcatgaa gcggatcttc
     1921 gcttggcagc acttaatcgt gaccttgcca accaaagtag taaataagaa tttgtcaatt
     1981 tgacacttta aataaaaagg agctcataaa ttattatgaa taaacgcgta aaaatcgttg
     2041 caacacttgg tcctgcggta gaattccgtg gtggtaagaa gtttggtgag tctggatact
     2101 ggggtgaaag ccttgacgta gaagcttcag cagaaaaaat tgctcaattg attaaagaag
     2161 gtgctaacgt tttccgtttc aacttctcac atggagatca tgctgagcaa ggagctcgta
     2221 tggctactgt tcgtaaagca gaagagattg caggacaaaa agttggcttc ctccttgata
     2281 ctaaaggacc tgaaattcgt acagaacttt ttgaagatgg tgcagatttc cattcatata
     2341 caacaggtac aaaattacgt gttgctacta agcaaggtat caaatcaact ccagaagtga
     2401 ttgcattgaa tgttgctggt ggacttgaca tctttgatga cgttgaagtt ggtaagcaaa
     2461 tccttgttga tgatggtaaa ctaggtctta ctgtgtttgc aaaagataaa gacactcgtg
     2521 aatttgaagt agttgttgag aatgatggcc ttattggtaa acaaaaaggt gtaaacatcc
     2581 cttatactaa aattcctttc ccagcacttg cagaacgcga taatgctgat atccgttttg
     2641 gacttgagca aggacttaac tttattgcta tctcatttgt acgtactgct aaagatgtta
     2701 atgaagttcg tgctatttgt gaagaaactg gcaatggaca cgttaagttg tttgctaaaa
     2761 ttgaaaatca acaaggtatc gataatattg atgagattat cgaagcagca gatggtatta
     2821 tgattgctcg tggtgatatg ggtatcgaag ttccatttga aatggttcca gtttaccaaa
     2881 aaatgatcat tactaaagtt aatgcagctg gtaaagcagt tattacagca acaaatatgc
     2941 ttgaaacaat gactgataaa ccacgtgcga ctcgttcaga agtatctgat gtcttcaatg
     3001 ctgttattga tggtactgat gctacaatgc tttcaggtga gtcagctaat ggtaaatacc
     3061 cagttgagtc agttcgtaca atggctacta ttgataaaaa tgctcaaaca ttactcaatg
     3121 agtatggtcg cttagactca tctgcattcc cacgtaataa caaaactgat gttattgcat
     3181 ctgcggttaa agatgcaaca cactcaatgg atatcaaact tgttgtaaca attactgaaa
     3241 caggtaatac agctcgtgcc atttctaagt tccgtccaga tgcagacatt ttggctgtta
     3301 catttgatga aaaagtacaa cgttcattga tgattaactg gggtgttatc cctgtccttg
     3361 cagacaaacc agcatctaca gatgatatgt ttgaggttgc agaacgtgta gcacttgaag
     3421 caggacttgt tgaatcaggc gataatatcg ttatcgttgc aggtgttcct gtaggtacag
     3481 gtggaactaa cacaatgcgt gttcgtactg ttaaataatt tttgaattca aacctattat
     3541 ttaagaatac cttaaataat gggtttttta tatgttttgc tttttattat tcgattattg
     3601 tgactagaat aggatatgat attaataatt accctccttt ttttatctat gaaaacatga
     3661 tataatggaa aaaagattag gagtagttat ggttaaacgc gattttatta gaaatattat
     3721 tttagcattg attgctgtat tgattttgat tctcttgaga tattttgttt ttgcaacctt
     3781 taaagtacat aaggatgcta caaattctta ttttagcaac ggagatgttg ttgttgttaa
     3841 tcgtaatagg acgcctaagt acaaagactt tattgtctat aaggttggaa agattttcta
     3901 tattagtcgt gttattggtg agccaaatca aaaagtgaga gtaatggatg atattttata
     3961 tttgaatgat gttttcaagg atgagcctta cattgaaaaa atgaaaaatg cttattctga
     4021 aaagaaagac ggtcaaatgc cctttacttc cgacttctca gttgaaacat taacacgaaa
     4081 taaagaaagc agagttccta agggttctta tcttgtgtta aatgataatc gacaaaataa
     4141 aaatgatagt cgaaaatttg gattaatcaa agagaaagat attcgtggtg ttattacctt
     4201 taaagtatac cctctaagtg aatttggttt tacagcatc
//
LOCUS       DATRNX010000131         4164 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRNX010000131 DATRNX010000000
VERSION     DATRNX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..750)
                     /locus_tag="VBC81_001818"
     CDS             complement(<1..750)
                     /locus_tag="VBC81_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6912826.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTH"
     gene            complement(762..1460)
                     /locus_tag="VBC81_001819"
     CDS             complement(762..1460)
                     /locus_tag="VBC81_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912827.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(1461..1829)
                     /locus_tag="VBC81_001820"
     CDS             complement(1461..1829)
                     /locus_tag="VBC81_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6912828.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1974..>4164
                     /locus_tag="VBC81_001821"
     CDS             1974..>4164
                     /locus_tag="VBC81_001821"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6912829.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIE"
BASE COUNT         1372 a          660 c          756 g         1376 t
ORIGIN      
        1 gtgtgtcaac atatagaata tagcaatttc aacaatgcta acaagtatat tgtagagcaa
       61 tacaggttga gagaatgttg catttgcatg agagttgaat aaatcaatat gaaaaatact
      121 atctattatt aatgttaaga aagtaattaa gagtgatatt ccaaaataag aaacaattcc
      181 catcaataca cgtttgtttg taaataattg ccctacagcg atagagagat aaatcagtaa
      241 tgtccctgca acgtacgata gaaagaaata ggcgataaaa agtggagcgt tcgaagatga
      301 aataatttta tagatttctg gaaaagcgac aacgaactga ccaataccta ctaaaggtaa
      361 tatgaccaat atgattccaa taatgacaat aaaaatatta aaaatactcc aaagaagcga
      421 agctagtagc tttgaacaaa taatctgatt cgtagttact gggagcgtta aggttagata
      481 tccttgacgg ccgaagatat tgctataaaa acgtttgata ataatagcaa gggtggataa
      541 cagagatcct gaaataacca tcgctaacaa taaaacgagt attatagtga atatttgggc
      601 gctagtacta ttagtatcag tagaaaaatt gccgccaagt gcctttaata ccagtcctaa
      661 aataatagat actaataaaa ccgcagcgtt gagtgttaga taccacttac caactgattt
      721 taattcatat tttaataatt ttccaaacat atcaatctcc tttatttctt gaatctttca
      781 cgaaagagag catcaatcga acagttgtgc tcttctctta agtcatcagc attgccttgt
      841 aggtcaattt ctccttcttt aaggaagata acttcatcaa ggataggttc aatgtcagaa
      901 attaagtgag tcgaaattaa aacagaagca tcattagagt agttgctaat gattgttttt
      961 aaaatgtagt cacgcgctgc agggtctaca ccaccaatag gttcatcaag gatgtagagc
     1021 ctagctttac gactcataac aagtattagt tgaactttct ccttgttccc ttttgatagg
     1081 ttttttagac gttccttagg atttaggtta agatcctcaa atagttgata agctaccttt
     1141 gaattaaaat cgttatagaa atcttcaaat aatgtaacga cgtctttgat tttcatattt
     1201 tcttgtaaat atgaagtatc agggagatat gaaataattt ttttggtttc aacagacggt
     1261 ctgtaaccat cgataacaat atctccctta tctggctgaa gtaatccatt tatcattttg
     1321 atgattgttg tttttccgct accatttgga cctaataaac ctataatctt tcctgttgga
     1381 atgcttacag tcacatcatt aacagcaata tgtttgtgat atttctttgt aacatgatga
     1441 agttgtaata actgtgtcat ttaattaatc tcctttataa aatgatcgag agcaaccgta
     1501 atttcatcta atttatatcc tatttttttc atgttgttga caaaagttgc taactctgct
     1561 tttgcaactt gttgacgaat tttaccaatt agtaagttgt cttctgtaac aaagcgtccg
     1621 gaagtacgtt gtgagaatac cattccttca cgttccaatt cagtgaaggc acgttgcatg
     1681 gtatttgggt taacaccagc ttcttgagct aactctctca cggtaggcaa ttggtcacca
     1741 ctttttattt cttgactaac aatttgcatc tttatatgtt cagcaatttg tgaataaata
     1801 ggtgattttt cattgaattc ccaagccata gataactcct ttcataatct tgtacttttt
     1861 gtactataaa aatagtacaa aaaaatattt gaaatgtcaa ggaattatca ttgagacttt
     1921 aatcgtttat tgtttcatag tataatagta aaaaatcgta aaaaggggct actatgtttg
     1981 cacaacttga tacaagaacc gtttattctt ttatggatag cttggttgat ttaaagacat
     2041 atgtctctaa atcgaaatca ttaggatatc aaactattgg tatcttggac cacagtaatc
     2101 tttatgcagc atatcatttt attcaagagg cgcaaaaggc aaacctaaga ccaatagttg
     2161 gatttagctt tgatatagtg gttgaaaata gatcaataga agtttactgt attgcgataa
     2221 atacagttgg ttataagaat ctcttgaagt tgtcaacggc tcaaatgtct gagaagatgt
     2281 ctctaaattt attgacagaa catcttgaag gggtgcaatt gattcttccc tatcaagatg
     2341 ctcttgatca gctcaattta ccctttgatt atgttattgg tgttaatttg acctctccgc
     2401 agattccata taccaaacct attatagcca tagatactgt tcgatatttt caaaaaaatg
     2461 atattgagac attacagatg cttcatgcta taagagataa tgtcctgtta aaagatgcac
     2521 aatatgcgag caaaaatcaa gaattaaaac cttgtcaaga aatgactcta gcattcaaga
     2581 aacgttttcc tgaagcttta gctaacctag aatctttggt agagaatgtt gtttatcact
     2641 ttgattctga ctttaagtta cctattttta atagagaaat tcctgctgga gaagaattaa
     2701 gaaccttaac tcaaaataac ttaaaaagta aaggtttatg gagcgatgct tatcaagagc
     2761 gtctcgaaaa agaattaaca atcattcaca aaatgggctt tgatgactat ttccttattg
     2821 tgtgggattt attaagattt ggtcgtagta agggctacta tatgggaatg ggacgtggtt
     2881 cagcagcggg aagtttagtc tcatatgctt taaatattac cggtatcgat cctgtcaaac
     2941 ataatttaat ttttgaacgt tttttaaatg aagagcgtta ttcgatgcca gatattgata
     3001 ttgatcttcc ggatatttat cgtggagaat tcttgcgcta tgtacgtaat cgatatggtt
     3061 caatgcattc tgcacagatt gtaaccttct caacttttgg tgcgaaacag gctattcgag
     3121 atgtttttaa acgctttggt gcatcagagt atgagttaac aaacataact aaaaaaattc
     3181 atttcagaga taatttgacc agtgtttata accgcaactt agctttcaga caaataatag
     3241 atagtaaaat tgaataccaa aaagcctatg atattgctaa gcgtattgaa ggaaatccta
     3301 gacagacttc tattcatgcc gctggggtag taatgagtga cgatttgtta acagatcata
     3361 ttcctttaaa aaacggtgag gatatgatga taacgcaata tgatgctagt tctgttgagg
     3421 ctaatggtct tctaaaaatg gactttctag gtcttcgaaa tttgacattt gttcagaaaa
     3481 tgaaggagaa agtcgataag gactacggta tctctataca attagaagct attgatttag
     3541 aagataaaga gactctaaaa ctatttgcgg ctggtcaaac aaaggggatt tttcaatttg
     3601 agcaaagtgg agctattaat ttgttgcgac gtattaggcc agagtgtttt gaagatgtgg
     3661 tagctactac cagcttgaat aggcctggtg caagtgatta tactgagaat tttataaacc
     3721 gtcgttttgg taaagaaaaa atcgatttag tagatcctgt catagctcca attttacaac
     3781 ctacctatgg cataatgctc tatcaagaac aggttatgca gattgcccag acctacgcag
     3841 gttttacgct aggcaagtct gatttgctca gacgtgctat gtctaagaaa aattctaagg
     3901 agatgcaaaa gatgtctcaa tccttcttag agggtgcagt atctaaggga catcgtcaag
     3961 aagatgctcg acttatattt gaacgcatgg ctaaatttgc aggttatggg tttaatcgca
     4021 gccatgcctt tgcttattcg gctttagctt ttcaattagc ctatttcaaa gcccattatt
     4081 ccgatgtctt ttatgacatc atgataaatt attcaaatag tgattatcta atcgatgcta
     4141 ttgattttgg ttttgttatt gaaa
//
LOCUS       DATRNX010000132         4160 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRNX010000132 DATRNX010000000
VERSION     DATRNX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(60..1622)
                     /gene="guaA"
                     /locus_tag="VBC81_001822"
     CDS             complement(60..1622)
                     /gene="guaA"
                     /locus_tag="VBC81_001822"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6912830.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1835..2533
                     /locus_tag="VBC81_001823"
     CDS             1835..2533
                     /locus_tag="VBC81_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6912831.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            2647..3981
                     /gene="trmFO"
                     /locus_tag="VBC81_001824"
     CDS             2647..3981
                     /gene="trmFO"
                     /locus_tag="VBC81_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6912832.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            4059..>4160
                     /locus_tag="VBC81_001825"
     CDS             4059..>4160
                     /locus_tag="VBC81_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912833.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFS"
BASE COUNT         1365 a          738 c          804 g         1253 t
ORIGIN      
        1 gtatccctta ttcaagaaat aagagtaaaa aaacttagct acttaaagta gctaagtttt
       61 tattcccact caacagttgc aggtggttta cttgtaatat cataaacaat acggttaacg
      121 tgatcaactt cgttaacaat acgtgtcgaa attttcttaa gtacatccca aggaagttgc
      181 gcaaagtcag ctgtcatgcc atcaattgaa gtaattgcac ggatagcaat tgtatagtca
      241 taagtacgac catctcccat aacaccgaca gaacgtacgc cagtattaac tgtgaaatac
      301 tgccatacgt cacgatctag accagcttta gcgatttctt cacgaagaat agcatctgat
      361 tcgcggactg tttcaagttt ttcttcggta atttcaccca taacgcgaat agcaagacca
      421 ggcccaggga atggttgacg ccatacaact tcatcaggca ttccaagtgc agtaccaaga
      481 gcgcgaacct catctttgaa taatgtgtta agtggctcaa tcaattcaaa ttgcatatct
      541 tctggcaatc caccaacatt atggtgtgac ttgattgttt gggctgtttc tgtcccagat
      601 tcaataatat ctgtgtagag ggtaccttga gcaaggaaat caacgccttt aagtttactt
      661 gcctcatcat caaaaacgta cacgaactca tttccgatga tttttcgttt tctctcggga
      721 tcttcaacac cagaaagaag atctaggaag cgttttgaag catcaacacg gataatatta
      781 agtccaaatt taccaccaag catatccata acttggtcac cttcattttt acgtaataaa
      841 ccatgatcta caaaaataca tgttaattga tcaccgatag cacgttgtag gagtactccg
      901 acaacagatg aatcaacacc gccagatagt ccaagaagta ctttacgatc gcctacagtt
      961 tcacgtattt ttgcaatttc catatcaatg aagttatcca ttgaccaatc accacgtgca
     1021 ccacagatat taacagcaaa atttttaagg atatcattgc catatactga gtgacgaact
     1081 tcggggtgga attggatacc ataaaattgt ttttcagtat tttccattgc agcaaatgga
     1141 cagtcaacag agtcaccaac gagatggaaa ccctcaggga tttcagtaac agcatcacca
     1201 tggctcatca aaacaagttg ttcttgagga gtgccagcaa agagtgctga ttctgagcgt
     1261 aggcgaagtg ctgattgacc atactcacgg tgaccagctt ctcccgcagg aagaacctta
     1321 ccaccgagtt tatgggtaat cagttgcata ccataacaaa ttcctaagat tggaattcct
     1381 aattcaaaaa tttcttcatc gattccaaaa gcgccatcag cgtatacgga atttggacca
     1441 cctgaaagta cgataccaat aggattaata tcgcgaattt catcagccgt aattttatgt
     1501 gattttaact cagaaaatac accaaattca cggatacgac gtgcgataag ctggttatat
     1561 tgactaccgt aatctagaac gataattttt tggatatcat tcaatataga aatatcagtc
     1621 attgtttccc tttctttgta gctaatttta aaaacagctg tacctatacc attttagcat
     1681 attttatata aattaacatc attaaaataa ttgattaaac gctttcttat caagaaatat
     1741 tgcttatgat ttttctgaat tatttcacga ttttagagtt agaaatatat agaagtatgg
     1801 taaaatgaag tgtagattct aggaggagag aatattgtta ccagcatata ttaaaattca
     1861 cgacgctatt aaaaaggaaa tcgataaagg gacttggaag attgggcagc ggttacctag
     1921 cgaaagagat ttagcggatg attatagtgt aagtcgtatg acacttcgcc aatcaattac
     1981 attgttagtt gaagaaggta ttttggagag acgagttggt agtggcacct atgttgctag
     2041 tcatcgtgtt caagaaaaaa tgcggggaac aactagtttt acagaaattg tcaattctca
     2101 agggcgtaag ccatccagta aactaatctc atttcaaaga aaactagcta atgaaaccga
     2161 aattcaaaaa ttaaatttat cacagtcaga ttatgtggtt cgtatggaaa gggtacgtta
     2221 tgctgataag gtaccgttgg tttatgaggt agcctctata ccagagaatt taattaaagg
     2281 ttttgaacaa tcagaggtta cagaacattt ctttaaaacc ttaacagaga atggctacga
     2341 aattgggaaa agccaacaaa ctatatatgc tagaaatgct agtgaacgtg ttgcttcgca
     2401 tttagaagtt aacgcaggcc atgctatttt ggcattgaca caagtttcat actttactga
     2461 tggtaagcct tttgaatatg ttcatgggca atatgttgga gatcgttttg agttttattt
     2521 agagaataac tagtagttta atgaataacg ttttttgaag aagtagctat gtatgttata
     2581 atggaaaaat aaattatata gaaagatttg aggaagtagt tgactacttt caagaggtat
     2641 tagttcatgt ctcaatctta cattaatgtt atcggcgctg gtttggctgg ttctgaagca
     2701 gcttatcaaa ttgctaaacg tggaatacca gttaagcttt atgaaatgcg tggggttaaa
     2761 tcaacccctc aacataaaac agataatttt gcagaattag tatgttccaa ttcattccgc
     2821 ggtgattctc taacaaatgc agttggtctt ttaaaagaag aaatgcgtcg tttggattct
     2881 atcattatga gaaatgggga ggcacatcga gttccagcag gtggagccat ggccgttgat
     2941 agagaaggtt attctgaagc tgttactgag gaaatccata aacatccatt aatcgaagta
     3001 attcgtgatg aaatcacaga tatccctgga gatgctatta cagttatcgc tacaggacca
     3061 ttaacaagtg atagtctggc agctaaaatc catgaactta atggtggtga tggtttttat
     3121 ttttacgatg ccgccgctcc aattgtcgat aaaaatacga ttgacatgaa taaagtttac
     3181 cttaagtcta ggtatgacaa aggtgaagca gcttatctaa attgtccaat gaccaaagaa
     3241 gaatttatgg cttttcatga agctttaact acagctgagg aagcaccact aaattctttt
     3301 gagaaagaaa aatacttcga aggttgtatg cctatcgaag tgatggctaa gcgtggtatt
     3361 aaaacgatgc tttatggtcc aatgaaacca gttggtttgg agtatcctga ggactacaaa
     3421 ggaccgcgag atggtgaatt taaaacacca tatgctgttg ttcaattacg tcaagataat
     3481 gctgcaggct ctctttataa catagttggt ttccaaactc atcttaagtg gggagaacaa
     3541 aaacgtgtat ttcaaatgat tcctggtctt gaaaatgctg aatttgtccg gtatggtgtt
     3601 atgcatcgca attcttatat ggattcacca aatcttttaa accaaacctt tgctacacgt
     3661 aagaacccaa atcttttctt tgctggacaa atgaccggtg ttgaaggtta tgtagaatcg
     3721 gcagcaagtg gtcttgtagc gggaatcaat gctgtccgtc gcttcaatgg ggagagtgaa
     3781 gtagttttcc cacaaactac tgctattggt gcactgccac attatattac acatacagac
     3841 agcaaacatt tccaaccaat gaatgttaac tttggtatca tcaaagaatt agaaggtcca
     3901 cgaattcgtg ataagaaaga gcgttatgaa gctattgcta cacgagcttt gaaagattta
     3961 gaaaaatttc tcaactattg atatattcat ttgactttgc caacaagcaa ggtcaaattt
     4021 tctatattga tttttaaaaa tgtatgaatg gagtaaatat gcactataaa gaattacttg
     4081 agtcatccga aaaagcttat ttggaaacta atcggtataa acattttaag aatataatca
     4141 cttcagagcg ttatttttca
//
LOCUS       DATRNX010000133         4094 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRNX010000133 DATRNX010000000
VERSION     DATRNX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(108..2372)
                     /gene="glgP"
                     /locus_tag="VBC81_001826"
     CDS             complement(108..2372)
                     /gene="glgP"
                     /locus_tag="VBC81_001826"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6912834.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(2384..3880)
                     /gene="malQ"
                     /locus_tag="VBC81_001827"
     CDS             complement(2384..3880)
                     /gene="malQ"
                     /locus_tag="VBC81_001827"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6912835.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
BASE COUNT         1285 a          780 c          614 g         1415 t
ORIGIN      
        1 taagttctgc tcagttctat ttttaaagtc tacggacaaa aataaatact tatagtttta
       61 ttagacaaga aaaaagtaag gttgaaaacc ttacttttag agacttttta atttgaatgc
      121 caaatatctt gattatattg ttcgattgta cgatctgatg agaagaaccc tgctttagca
      181 atattgtgta taactttctt attccatgat tcataatctt cataatcagc aagcacttgt
      241 tctttaacag caatgtattc tttcaaatca atcaatgtca tgaaccaatc tttacctact
      301 aactcatcat gcaatctctt tagtcgttct gcattaccga gtgacactat atcatcacta
      361 atgataaaat caacagcttc acgaattact ttgtctttat catagtaatc ttttgaacga
      421 taaccagatg tttcataaag attaatgata gtatcagagt ctttaccaaa tgtgtagatg
      481 ttctctttgc cagctaattc agctatttca acatttgccc catccatagt accaagagta
      541 agagcaccat tcaacataaa tttcatgtta cctgtaccag atgcttcttt ggaagccaat
      601 gaaatttgtt ccgaaatatc tgttgcaggg attaactttt ccgctactgt cacattgtaa
      661 ttttctacta aatggacatt aaggtattta ttaacttctg gatcattatt aattaattct
      721 gataagcaga gaattaagtg aatgatatcc tgtgcaatag tataggctgg agcagcttta
      781 ccaccaaaaa taactgttaa tttacgtgat gggaaatgcc cacgtttaat ttctaagtat
      841 ttatgaataa catataaagc attcatttgt tggcgcttat attcgtggaa tcgtttgatt
      901 tgtgtatcaa taattgagta ttcatccaat tcaatacctt tattttcctt aaggtaacgt
      961 ttaagagcca gtttgttttt aaatttaata gcagctaatt tatcatgaac ttcattgcta
     1021 tcagcataag ttaacaattt ttcgagctga gtcgcatcag taagatagct atccccaatt
     1081 agttctttca agtaatctgc taaatcttgg ttagcaaact ccaaccagcg acggaatgta
     1141 ataccgtttg tcttattgtt aaacttatct gggtaaatat catagaaaac ctttaattca
     1201 ctatttttaa ggatttctgt atgtaatgct gctacaccat taacgcttgt tgagaagtgg
     1261 atatccatat gcgccatgtg aacacgtcca gcttcatcaa taatttgaac ctctggattt
     1321 gtttgctctt cacgaatcat ttgatctaat tttttaatga ttgttactag gtggggtact
     1381 acctcattaa gatactctaa aggccatttt tcaagagctt ctgcaaggat ggtatggttt
     1441 gtatagccaa ccatattgcg aacaactgac acagcttcgt caaattcaaa accatgtttt
     1501 tctgtcaata aacgaatcaa ctcagggatg accatagatg ggtgagtgtc attaatttga
     1561 acataagcat attcagctaa atcatgaagg tttgaaccac gctcgattgc ttcatcaatt
     1621 aataattgag cagcatttga gaccatgaaa tattgctgat agatacgtaa taattctcca
     1681 tttttatctg aatcatctgg atacaagaac aaggttaaat tcttcttgat ttctgtctta
     1741 tcaaaagtga tacctttttc aataaggtta taatcaagtc catcaatatc aaaaaggttc
     1801 aaataatttt tagtatcttt cttataacct aatacatcaa ttctatctag gcgtgatttt
     1861 aaagtaaagt cacggaaagg aacatcataa gaaatatctg ttggtactaa ccaagaatta
     1921 ttctcaatcc agtagttagc ttctgcttct tgttgattgt tgcgaaatac ttgtttgaaa
     1981 agcccacagt gatagtttaa accaacacct tcaccattaa tacctaaaga agaaatcgag
     2041 tcaataaaac atgatgctaa acgccctaaa ccaccattac caagtgatgg ttctggctct
     2101 acatcttcta tttcagcaat tgatttacct acaagttcta actctttttt gacatcttta
     2161 taaatcccta agttaatcaa attatttgaa agtaatttac caattagaaa ctcagctgaa
     2221 atatagtata ccttacgttg agagctattt tttgatttag cggcagcttc ttcttttaca
     2281 aaatttaaca attcaacgta aatctcttca tttgttaatt ctgataatac ttttccttgt
     2341 tgtccaacgt aagttgtaaa atttcttgtc atgataatat tccttattta ttccctctat
     2401 tataaatagt tgtaatctct tttaagaatg ctttgcgatt ttctgttaag tcttcttttc
     2461 tcatacgcca ttgccagtta cctccaacgg tatttggcat gttcatacgg ctatctgcag
     2521 gtttatctag tagatcttgc atacatgtaa tcgttgtttg actaacggtt gcatacaagg
     2581 tacgcagtac agtctcagta atgggctcat ttggcaaacg acgcatgtaa ttttctgcat
     2641 aagctttctg ttcgactgta agattttcaa accagccatt aataacttcg ttatcatgag
     2701 ttccagcata tgcaattgta ttttctgtgt agttatgagg aatatcaata ctatttcctg
     2761 ttgtatcata aaaaccgaat tccatgattt tcatacctgg gaaccctgtt ccagccagta
     2821 atctctctgc tctctcatca atatagccta aattctcagc aataataggt aaatcaccta
     2881 atttttcttt gatagttgca aataattctg gaccgggagc tggttgccat gagccatcat
     2941 tagctgtttg ataatctcct cgaatttccc aataatctga aaaaccttta aaatgatcaa
     3001 tacgcaaata gtcatacatc ttaacaccag attgaatacg ataaatccac cagtcaaaat
     3061 ctgactcttt gtggtaatcc caattgtaaa taggattgcc ccaaagctga ccatcgtcag
     3121 aaaaatcatc cgctggtaca ccagcaatag caagtggttg tttgtcgcaa tctaatttaa
     3181 acagttcagg cattgtccaa acttctacac tatcggcaga aacgtagatt ggcatatcac
     3241 cgataatttg aatcccttta tcattagcat attcttttaa ctcaaaccat tgtttgtaaa
     3301 agaaatattg cgttacttca tgatatttta tcacttcact aagcttttga cgatatcctg
     3361 ctaaggcttc ttcttcgcgg cgtataatag ccttgtcatc ccattcttga agcgccttat
     3421 taccaaaatg ttctttgatc gccataaatt cagcaaaatc agttacccat ttttcttctt
     3481 gcaagaaatc agatagcatt ctcgtagctc tctcttcttg caagaaattt ttaactgctt
     3541 tttctaaaac tggacggcgt ttttcaaaca gaccagcata atcaacaact tctggatcct
     3601 gtccaaaact aatattttgg tagtcatctt tcgaaatgaa accttcaagc gttagcaaat
     3661 caaaatcaat taaatgtgta ttaccagcta ctgcagaaaa agactggtaa ggagagtccc
     3721 cgaagcttgt tgttgttaag ggaagaattt gccaaaattt ttgatcagtt tcgaccaaaa
     3781 aatcaacaaa agcgtaggct tctcgtccaa atgttccaat acctaaatca cctggtagag
     3841 aagtgatgtg cattaagaca ccacttgcac gttttttcat attgattctc cttttcgatt
     3901 acttacttta ctcctccatc ttaacgcaag cgattgcata agtcaagcat aatttaaaaa
     3961 aacttctatc gaaaattgat agaagtcttt atatcatagc ttaattatta tttttagaaa
     4021 tatctcggac actttcacgc tcttctaaag taaaaggcac aataatagtc tcactaaaga
     4081 ctttgtctgg agat
//
LOCUS       DATRNX010000134         4052 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRNX010000134 DATRNX010000000
VERSION     DATRNX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(20..787)
                     /locus_tag="VBC81_001828"
     CDS             complement(20..787)
                     /locus_tag="VBC81_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6912836.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(796..1506)
                     /gene="deoD"
                     /locus_tag="VBC81_001829"
     CDS             complement(796..1506)
                     /gene="deoD"
                     /locus_tag="VBC81_001829"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6912837.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            complement(1490..2746)
                     /locus_tag="VBC81_001830"
     CDS             complement(1490..2746)
                     /locus_tag="VBC81_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6912838.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            complement(2748..3557)
                     /locus_tag="VBC81_001831"
     CDS             complement(2748..3557)
                     /locus_tag="VBC81_001831"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6912839.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            complement(3596..4003)
                     /gene="arsC"
                     /locus_tag="VBC81_001832"
     CDS             complement(3596..4003)
                     /gene="arsC"
                     /locus_tag="VBC81_001832"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6912840.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
BASE COUNT         1329 a          798 c          662 g         1263 t
ORIGIN      
        1 tctcagaact gtttttaaat tactcaagta tctttataat aagcttgtta accgtatcgt
       61 agtgaaaatt aacagtatca tactcaacac caattacctt tgccaagaag ataatctggt
      121 ctaaaaatgc ttgagaagaa aattgagttg tacgatatag ttcttgctca tcaaaaggtt
      181 taatcaggtg agccaaagga tttctgactg atttttcgaa ctgtcttaat tgaatgatgg
      241 tcgatttcac ttcttcatca aggtcggtta attgtaaaca ttctgcaagg cttttagaag
      301 tcaagcgact atcgtgccac ctttcagaga atgccaaaag gactggatgg tttgactcct
      361 ttaattcttt aaacttccaa gtatcgtagt gatcatctct tgcattatag atgtaatcat
      421 gcaagtgagg tacttcttgt gcaagtaaaa tcataaatag acgatataaa ataggactaa
      481 cactacggac aaaatctatg attaatccat tttttacttt tgcttctaaa tccatgatgt
      541 aatttgccag caactctaaa tcatacgagt tatgcaaaag ctttatattg tatttttctt
      601 gtaatataac aacttgttct tgatgagcaa aaagaaaatc aatgtttaac tctcgacgct
      661 ctttcatcat ttctaataaa aataataaat ctgagtttaa aggaccatgt tttcgtaata
      721 ataagaaagc attatgataa gaatactgtt ccactaatac tttaagagca gttgaaaaat
      781 ctgtcatttc tatcctcatt cagaaataag tgtttcgagc ccaactttca tcatatcagt
      841 aaatgttgtt tgacgctctt ctgcacttgt atcttcttct ggattattca agttgtctga
      901 aatcgtcatc atagcaagag cattaacatt gtgttgagct gcaagatagt agagagctgc
      961 tgcttccatc tcaatagcat gaacacctag tttacccaaa gccatattac gatctggttg
     1021 gtttgaataa aacacgtctg aagataatac actgcctaca tgagttgtaa tatccatttc
     1081 ctttgcgatg tgataagctt tatccaaaag tttaaaatca gcaatttgcg gaaaatcaaa
     1141 ttcaggccaa tcattacgga taatattaga atttgttgct gctgcttgtg ccaaaacaag
     1201 ttcacgaaca tggatatcag gattaatagc acctgctgtt cctacgcgaa tcaatgtttt
     1261 aaccccataa tcaacaatta gttcgcgagc ataaattgaa attgaaggca tacccatacc
     1321 agttcccata atagaaacac gatgtccttt ataggtacct gtataaccaa acatattgcg
     1381 aactgtatta aaacacaccg catcttctaa aaaattctcg gcaataaatt tcgcacggag
     1441 tggatctcct ggaagtaaaa ttttatctgc aatttcacct tgttttgctt caatatggat
     1501 tgacatataa actcctcatt tttcttataa aataatcata ggcagtagca aaaagtactg
     1561 ctatagttag gggaacaata gttataacag attgaccagt aaaagatatg actaaaccga
     1621 cagctgtcag tggcgctctc attgtaatag ctaaaaaaat agctgcacca accaacatac
     1681 tggttactat cgataggtgg ggaagtaaag gaatagaaat actagctaac aaaaaaccta
     1741 aacaagcacc aatggaaaaa gatggtgtca gtataccgcc ataagcacca gcgtaaagag
     1801 tggataatgt tgctatcatc ttgagaacaa ctaaaatagc gattaagcta actgtggtcc
     1861 ctttaaagac ttcttgaact agggcattac catttcctaa aatctgggga tagattattg
     1921 ataaaacaga tactatcaat gacactactg gcaaacttaa caatattttt atatttttaa
     1981 tacgtctctc tgtcactttt tgatttaaat atctaaagct aatcgcaatc ggagttacac
     2041 ataaaacgat caagaacata aaagctaatg actgtgaatt cagactcatc tttgaaatat
     2101 gataaagagg ggctgtcgaa ataactaatt gagcaatact agctgcacca aaagtagaga
     2161 ctagcaatgt cacaatattt ttcagtgaaa ttcctaaagc taaagtttca aaagcaaaga
     2221 atacactagc taagggtact tgataaactg ctgacaaacc tgcagctgca ccactaacga
     2281 ttaataaacg tttatccagc actttaagtg ctaataaatc cgatattttc ccagcattta
     2341 tggctccaaa ctctcgtggc gctccttctt ttccaatagg accaccagct cctacataaa
     2401 tcaattgcaa aaatgcgtga cctatatgta gccagtaagg tgattttttt acagaaaaat
     2461 ccttaaaaac aacctgttgt ttaatactag ttatttgctt tggcttgatt tgtaagtaat
     2521 accaactaag ggatgctaaa aaggagacta aagtaagtga taacactctt ttaattggtg
     2581 tgacactatt taacaaagac agagtatttt ctctactatc accaaatgct aaccactctg
     2641 taaacattaa tacatagtgc attaagatag ctccaactcc tgccataact gtcataaata
     2701 gaacggctat cataaacttg acagcatagt ataatcgaga aaccatttta aagttcagca
     2761 actaatgatt taactaatcc cttgaaatct tctttaatac gctgagtaac ttcaacgacc
     2821 tcctcatgat tgagctctga ttggaagcca gcggcaaagt tagtaattgc tgaaattcct
     2881 aacactttaa gccctgagtg agctgcaacg atcacctctg gaaccgtgga catacctacc
     2941 gcttgtgcgc ccattgtttg gaatgcacga atttctgcag gtgtttcata agtgggtcct
     3001 gatacaccca agtacacacc ttcttcgagt ttgatattct gtttttcagc aatttggtga
     3061 gctttttgtc gatatgttgc tgtataagca tccgacatgt ctgggaaacg tggtccaaat
     3121 tcttcaaggt tctcacctat gagagggtta gtcccaatca tattgatgtg gtctttgatc
     3181 agcattaaag ttcctggtcc gtatccaatc ccacccgctg cattagtcac aagcacactg
     3241 tggcaagcca atgctctcat gatacgtact gggaaagtaa cgacttccat tgtatttcct
     3301 tcataaaagt gaaaacgacc ttgaagcgct aataccttac ggcctgataa atccccatac
     3361 actaattttc cagcatgacc aactactgtt gactgtcccc aatttgggat gtctgcataa
     3421 tccacaacaa tgggattttc gatttcttca gctaattctc ctaaaccaga gcctaaaata
     3481 agaccaaatt ctggtgctgt gacgcctttt gcttgcaaaa agtctctagt ctcattaatt
     3541 ttttctaata atgtcattta cttacctcct aaactattat tcactataca atatgctata
     3601 aactttggat aatctgccca tcttctttaa caaaatcttt ttccatcttt acaggtaaaa
     3661 tggttaaaat ttcttcacag ggtcggcaga gttttgcacc tttactcgta acaactatgg
     3721 gacgattgat aagaacagga tcttgtatca tcgcatcaat catttcttca tctgtaacgc
     3781 tagaagattc caaattgaat ttctcataag caggaacatc tgttcttaga agttctctca
     3841 cagaaagttt catttctaac aacagttcaa ctagtgccat cttgctaggt ggtgttttca
     3901 gataataaat aatttctggt tcaataccac aatgacgaat aatggcaagt acgttccttg
     3961 atgtcccaca atttgggtta tggtaaatcc taactttttc catcatcttc ctctcaattt
     4021 ttaatgctac aagtgtctta aggaaataaa aa
//
LOCUS       DATRNX010000135         3928 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRNX010000135 DATRNX010000000
VERSION     DATRNX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..764)
                     /locus_tag="VBC81_001833"
     CDS             complement(<1..764)
                     /locus_tag="VBC81_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEO6912841.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNN"
     gene            934..1536
                     /locus_tag="VBC81_001834"
     CDS             934..1536
                     /locus_tag="VBC81_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6912842.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            1583..2989
                     /gene="pepV"
                     /locus_tag="VBC81_001835"
     CDS             1583..2989
                     /gene="pepV"
                     /locus_tag="VBC81_001835"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6912843.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            3086..3670
                     /locus_tag="VBC81_001836"
     CDS             3086..3670
                     /locus_tag="VBC81_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6912844.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            3685..>3928
                     /locus_tag="VBC81_001837"
     CDS             3685..>3928
                     /locus_tag="VBC81_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912845.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRE"
BASE COUNT         1331 a          615 c          774 g         1208 t
ORIGIN      
        1 aaattgtttc cagaatgatc aatgaatgaa aatgtaccag caacaacagc tagtgcaact
       61 gttccgatta cagatataaa taaactggtt aaaataattt tacttagaag acgccactga
      121 cgctttccgc ggctaagttt ccaactctct aaaagtgctt ctttagcatt gtagtcagtt
      181 aaaatggtca gcgggagaaa ataaattaat cgaaggttta agtaacaaat aagcagtaaa
      241 agggtagagt aacctatctt tccaaagact gtctttgtta actcgccaga aataaagtca
      301 ggaattttaa gatgctctaa tatacttgac cttagaccaa ggttagctag aggaattgtc
      361 aaaagtaagt agattgatac ccaaattagc tgtggtccta aaagtgattt tattttttta
      421 ggaaagattg ttagccattt tttgatactg agagaaaatt cagttttcag tatggcaata
      481 gtatagatag cgaactcagt atagatgaac ccaattagga ttacgatata aagtataaag
      541 agtaataaag ctaagggatg attaaaaagc aaagtccaat tgtctttagt cactgcggga
      601 gtatttgata atatcaatat ccgacgaaag ataaaactca gaattccttt tactaaataa
      661 gcaactaaag tttggattaa gataccagtt ccaatgtaaa cccaaaattg attttttaaa
      721 gaggttaagg ttagaaaaaa atcttctttt aatgctcgta gcatgaatag ctcctttttt
      781 atagtcatta gatattgtac cacaagtttg tttgtttttt atggtagttt tcaaaccggt
      841 ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc attttaaaaa gtgatagaat
      901 agtagggaaa atcaaataga ataggaggtt tttatgaaat ttctagaatt aaataagaag
      961 cgccatgctg tgaaacattt taatgataag ccagtcgact ttaaagatgt tagaactgct
     1021 attgaaattg cgacattagc gccatctgct aataatattc agccttggaa gtttgttgtt
     1081 gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg aaagtaattg taaccaaatt
     1141 aatcaagcgc agtatgttat tgctttattt acagatacag atttaggaca gagatcacgg
     1201 aaaatagctc gtattggtcg tagatctctt cctgatgatt taattggtta ttatatggaa
     1261 actctaccac cacgctatgc tttatatagt gaaaaacaaa ctggagagta tctctcttta
     1321 aatgcaggta tcgttgctat gaatttagta cttgccttga cagatcaagg tattagttct
     1381 aatatgattt tagggttcga taaagcgatt accaatgatg tcttagagat agataaacgt
     1441 tttagaccag aaatattaat tactgttggt tatagtgatg aaaaggttga gcctagttat
     1501 cgtttgccag ttgatcatat tattgaaaaa cgttaatttt taagcacaaa ctacataata
     1561 ttttatagaa ggagttgccg taatgacaat tgattttaga gcagaagtag acaaacgcaa
     1621 agacgctttg atggacgatt taataaatct tcttcgtatt aactcggagc gtgatgattc
     1681 acaagctgat gcagagcatc catttggtcc tggacctgta aaagccttgg aatttttcct
     1741 tgagatggct gagcgagatg gctacgaaac aaaaaacgta gataattatg cgggacactt
     1801 tacatttggt caaggcgaag aagaactagg tatttttggg catttagatg ttgttcctgc
     1861 aggcagcggt tgggatacag acccttatga accagttatc aaggataacc gtttatatgc
     1921 tcgagggtca tcagacgata agggaccaac aatggcatgt tattatgctc taaaaattat
     1981 taaagagctt gggctaccaa catctaagaa agttcgtttt gttgtaggaa ctgatgaaga
     2041 atctggatgg ggagatatgg attactactt cgaacatgtt ggtcttccaa aacctgattt
     2101 tggtttctct ccagatgcag aatttccaat tataaatggt gaaaaaggta atatcactga
     2161 atatttacat ttttcaggag aaaataaggg agctgttaga ctgcatagct tttctggcgg
     2221 acttcgcgaa aacatggttc cagaatcagc cacagcacgt ttcacaagtc atttagatca
     2281 gacaacactt ggagctagtc tagctgattt tgcaggtaaa cataatttga aagcagaatt
     2341 atctgtagaa gatgggcagt atatagctac agtttatggt aaatctgctc atggttcaat
     2401 accacaagaa ggtgttaatg gggcgactta tttagctctt tatctaagtc aatttgattt
     2461 tgaaggtcct gctcgtgctt tcttagatgt cacagccaac attattcacg aagacttctc
     2521 aggtgaaaaa cttggagtag cttatgaaga tgactgtatg ggatcgttga gcatgaatgc
     2581 aggtgtcttc cagtttgatg aaactaatga tgataatact atcgctctta atttccgtta
     2641 cccacaaggg acagatgcta aaactatcca aactaagctt gagaaactta acggagttga
     2701 aaaagtgact ctttctgacc atgaacacac accacactat gtacctatgg acgatgaatt
     2761 agtatcaacc ttactagctg tctatgaaaa gcaaactggt cttaaaggac atgaacaggt
     2821 tattggtggg gggacatttg gtcgcttact tgaacggggt gttgcatacg gtgccatgtt
     2881 cccaggagat gaaaacacta tgcatcaagc taatgagtac atgcctttag aaaatatttt
     2941 ccgttcggct gctatctacg cagaagctat ctatgaatta atcaaataaa ataatcctta
     3001 aactaaatat gtgatcaatg ataaagggtg gtgaagacat gaaagtgtct ttgcctcttt
     3061 tcataaggtt agatttggag actttatgac tgacttggaa aaaattatta aagcaataaa
     3121 aagtgattca cagaatcaaa attatacaga aaatggtatt gatcctttgt ttgctgctcc
     3181 taaaacagct aggatcaata ttgttggaca agcacctggt ttaaaaactc aagaagcaat
     3241 actctattgg aaagataaat ctggagatcg tctacgccag tggcttggag ttgatgaaga
     3301 gacattttac cattctggaa aatttgctgt tttgccttta gatttttatt acccaggcaa
     3361 aggaaaatca ggagatttac cccctagaaa aggttttgcg gagaaatggc accctcttat
     3421 tttaaaagaa atgcctaatg ttcaattgac cttgctagtt ggtcaatatg ctcagaaata
     3481 ttatcttgga agctccgcac ataaaaatct aacagaaaca gttaaatctt acaaagacta
     3541 tctacccgat tatttacccc tggttcaccc atcaccgcga aatcaaattt ggctaaagaa
     3601 gaatccatgg tttgaaaaag atctaatcgt tgatttacaa aagatagtag cagatatttt
     3661 aaaagattaa ggataggagt tagtatgaga gataatcatc tacacacgca tttttcctat
     3721 gattgtcaaa cggcatttga ggactatatt aatggtttta caggtgaatt tatcacaaca
     3781 gaacattttg atttatcaaa tccttacacc ggtcaagacg atgttcctga ttatagtgct
     3841 tattgtcaaa aaatagatta tcttaatcag aaatatggaa atcgatttaa aaaaggaatt
     3901 gaaatcggtt attttaaaga tagggaat
//
LOCUS       DATRNX010000136         3926 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRNX010000136 DATRNX010000000
VERSION     DATRNX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..330
                     /locus_tag="VBC81_001838"
     CDS             <1..330
                     /locus_tag="VBC81_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6912846.1"
                     /translation="TIFNLPIPGSIIGLILLFLALKYKIIRLRHIDTVGNFLLANMTI
                     LFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILTIGLVSQWIKKRYEGDYPEIGG
                     KNGNFNE"
     gene            311..1006
                     /locus_tag="VBC81_001839"
     CDS             311..1006
                     /locus_tag="VBC81_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6912847.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIDDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            1075..1698
                     /locus_tag="VBC81_001840"
     CDS             1075..1698
                     /locus_tag="VBC81_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6912848.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(1967..2038)
                     /locus_tag="VBC81_001841"
     tRNA            complement(1967..2038)
                     /locus_tag="VBC81_001841"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2003..2005),aa:Arg,seq:ccg)
     gene            complement(2111..3313)
                     /gene="rpsA"
                     /locus_tag="VBC81_001842"
     CDS             complement(2111..3313)
                     /gene="rpsA"
                     /locus_tag="VBC81_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6912849.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(3439..3510)
                     /locus_tag="VBC81_001843"
     tRNA            complement(3439..3510)
                     /locus_tag="VBC81_001843"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3476..3478),aa:Gln,seq:ttg)
     gene            complement(3516..3596)
                     /locus_tag="VBC81_001844"
     tRNA            complement(3516..3596)
                     /locus_tag="VBC81_001844"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3560..3562),aa:Tyr,seq:gta)
     gene            complement(3657..3887)
                     /locus_tag="VBC81_001845"
     CDS             complement(3657..3887)
                     /locus_tag="VBC81_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6912850.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
BASE COUNT         1271 a          779 c          635 g         1241 t
ORIGIN      
        1 actatattca acttacctat accaggtagt attattggat taatattact attcctagcg
       61 ttaaaataca aaatcatcag gctcaggcat attgatactg tggggaattt tctattagca
      121 aatatgacca ttttattttt accaccagca gttggactga tggagcactt tcaagacata
      181 aagccctatc tttttgggat agctatcatt atcttaggag cgctatttct caatattcta
      241 actattggac tggtatccca atggatcaaa aagagatatg aaggagatta tccagaaatt
      301 ggaggtaaaa atggcaactt taacgaataa ccctatcttt ggtattatgt taaccgtatg
      361 ggcgtattac attggcataa ggatttttag aaaatatcct tcccctgcaa caactccact
      421 tcttttggcg actattttat taattgcttt tttaaaatta acacatatct cctataaaga
      481 ttactacaat ggtggttcat ttctaacgat gttaattacg ccttcaacgg ttgtattggc
      541 tattcctctc tatagaacct ttcatttaat gaagcatcac attaaaagta ttagcattag
      601 catcatctta gcttctgtta tcaacacagt ttttaccgca atagttgcta agttttttgg
      661 tatgaaatat ttcttagcaa tctcactttt cccaaagtcc gtaactacag ctatggccgt
      721 tggaattaca agtaaagcag gtggattagc tactatcact ctggtcgtag ttgttattac
      781 aggcatctta acaagcgttt tggggcctat ttttctaaaa ttattaaaaa tagatgatcc
      841 tgttgctata gggctcgcgc taggaggaac tgggcatgct ataggaactg gacaagcctt
      901 aaaatatggc caagttcaag gcgccatggc tggactagca attggtatca caggaatttg
      961 ctatgttatt gtcagtcctt tagttgcagg cttaatattg aaatgaggaa aagaaaagta
     1021 aacccaaaat aattatgtta taatatttaa aaatagaatt taggataaat aattatgata
     1081 ccgttaaaaa aaataactgt tggaacgatt gtctgtctct catttttggg tttaacagca
     1141 tgctcttctt caaatactca gcaaacctct acttcaaaat caaatgtatc acaacataaa
     1201 aatatcaaag ctgaccacga agaattacgt ttgaaattta ataaagtaaa gttaggggtt
     1261 aaagcaaata actttaaagg aggcactagt ctcgctgaat taaaacaatt atttggtggc
     1321 gaaccaaatg aaaaatttga tacaccagcg ggaaatgtca ctcttaaagg ctatcgttgg
     1381 aatgtcgatg atatctccat cactatacaa ttattaaacg atagtagcat tgttcgttca
     1441 attagtaatt ttaagtttat tcgtgatgcc aacatcacaa caaaagatta taatagttta
     1501 aaaaatggca tgtcatacaa taaagtaaaa gaacttctag gagaacctga tgatatttct
     1561 caagcagttt cttcagataa agaggaacta caggctgcat ggattagtgg catccagtcg
     1621 tcagacagtg acccgggaat taaccttaca tttgaaaatg ataaactcac taacaaacaa
     1681 cagcatggtt taaaataaaa aggcattaac gcctttttat taatacctta attacttatt
     1741 agaaacattt tattacacaa tggtttaatc ttagtacctt aacttagccc cttaatggtc
     1801 atactaacta attatgatac taacttacac agatttcaaa ctgattctcc cttatttgtc
     1861 aaccctagtt ttacttatca gaaatgagga gaatcagtct gtgaggcaag gttggagata
     1921 aactaatata aagcctataa cataaagctt tatggagcat ctattatcca tcttaaggga
     1981 atcgaacccc tatcccaaga accggaatct tgtgtgatat ccattacact aaagatggta
     2041 gtacatgtta cattatagca tactttatca acaaaaacaa aaagacttgc tttagcaagt
     2101 cttttaatta ttataattca atatcaccga ataagtcagc catagagaat ccagtttgtg
     2161 tttctggaag ctcatagtcg cgtttttctt gacgacgtgg acgacgtgga cgtgattgac
     2221 gtttttcttc tttttcacct tctgcttgcg ctggacgctc ttcaagagct ttcattgaaa
     2281 gtgatacacg ttctgcatct gaattaactt caagaacttt aacagtaact tcttgtcctg
     2341 cagataatac gtctttagga ttttcaacac gtttgtgtga aatttgtgaa atgtgtacaa
     2401 gtccatcaat gccaggtaac acttcaacaa aagcaccaaa gtcagtcaag cgttttactt
     2461 taccttcaat aacatcacca gcagcaagtt tttgttcaac gccatcccat gggccaggtg
     2521 tagttgcttt aagtgaaagt gatacacgac cagcttcttc atcaattgaa agtactttaa
     2581 cttcaacttc ttcaccaaca gttacaactg atttaggtga aacattgcgc tcatgtgata
     2641 attcagttac gtgtacaagt ccatcaacac caccaaggtc aatgaaagca ccaaagcttg
     2701 ttaaacgagc aactttacca gttacaactg aacctacttc aatgtttgag aaaacttctt
     2761 tacgtgcagc agctgctgat tcttcaacaa cttcacgacg tgaaaggatg aagcgatttt
     2821 ctgctgcatc aacttctttg attttagcat caaattcttg tccaacaaat ttttcagtgt
     2881 tacgtacaaa acgagtatca atcattgaag ctgggatgaa accacgaagt ccttcaaatt
     2941 caactgaaag tccgccttta acagcacgtg tacctttaac agtaacaact tcaccttcac
     3001 gaccaacaag tttgtcccat gctttacgag cttccaagcg ttttttagat acaaggaaag
     3061 ttactgtatc agtatcttta cctacaactt gacgaagaac aagtacttca actgtgtcgc
     3121 cagctttaac aaaatcattg atatcagcat cacgatcgtt agtcaattca cgaagtgtca
     3181 agacaccttc aacaccagta ccatcgataa caacgtttgc ttgaccgtta tcaactgtta
     3241 aaacttccgc agtgacaacg tcaccaggat tcacttcgct aacactgttt agcaaatctt
     3301 caaattcatt cattctaaaa aatcctccaa caaaagctag ccgagctagc ttttgataac
     3361 aatatatttt ctcaaggtaa cccgcaagga caacaaatat ctctttgacg gtgtcaaaca
     3421 aaagtttgat acctttgact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag
     3481 tcccttgcct taccgcttgg ctatacccca aataatggaa agagagggat tcgaaccccc
     3541 gaacccgaag gagcggattt acagtccgcc acgtttagcc tcttcgctat ctttccgtaa
     3601 caacactgac tattctacca cagatgttgt tagcacgcaa gctttttaaa taaatattag
     3661 cgattaagat tatattcttc aataatattt cccatagctt gatctaaatt tttatagaat
     3721 ccactcactt gctcaccttt aatcacagca aatttagaat ctagctctgc aatttgacct
     3781 acaatttttt ttccaataac gagttggtat ccatcaacct tagttccatt aactgttact
     3841 tgagcgtcta aaatttgaat ttcaattttc ttatcttttt tactcactat taactccaat
     3901 atctctttta ttgtttttta ttgtac
//
LOCUS       DATRNX010000137         3877 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRNX010000137 DATRNX010000000
VERSION     DATRNX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            103..1248
                     /locus_tag="VBC81_001846"
     CDS             103..1248
                     /locus_tag="VBC81_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912851.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1248..1883
                     /locus_tag="VBC81_001847"
     CDS             1248..1883
                     /locus_tag="VBC81_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912852.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1886..2812
                     /locus_tag="VBC81_001848"
     CDS             1886..2812
                     /locus_tag="VBC81_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6912853.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2813..3463
                     /locus_tag="VBC81_001849"
     CDS             2813..3463
                     /locus_tag="VBC81_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912854.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3525..>3877)
                     /locus_tag="VBC81_001850"
     CDS             complement(3525..>3877)
                     /locus_tag="VBC81_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912855.1"
                     /translation="SMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFN
                     IHFFSQVQIRVDEAYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIA
                     ILGGLGFSYSKKQF"
BASE COUNT         1283 a          686 c          668 g         1240 t
ORIGIN      
        1 ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacata ttgccaaagt
       61 tttgatatta ttactataat atagtttgta gaggagaata atatgggcca agaacctatc
      121 atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga agatattaac
      181 cttaaaattt accctggtga tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca
      241 acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct attatttaag
      301 ggaaaagata tctctactat taaccccatt gaattaagac gcagaattgg atatgttatc
      361 caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct tgtaccaaaa
      421 ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat taaattagtt
      481 gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa
      541 cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat ggatgagcct
      601 tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa gtctcttcag
      661 gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc cctcaagtta
      721 gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac acccaatgat
      781 ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga gcgtctcctt
      841 catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc tgtttcaata
      901 actactgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg cgttgactca
      961 cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc tctaagcagt
     1021 aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt ttatgttatg
     1081 gaagacgact tacttagaaa tactgctgag cgtattttaa aacgtggttt aaaatacgct
     1141 ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc cctagttgat
     1201 atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg gtcaatttct
     1261 tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat atctcatttt
     1321 ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta acacgttttc
     1381 ccaaagtagc caaaattatc attgctatag ctagcatgct acaaactata ccaagtttag
     1441 ccttattagc tttaatgatt ccattgtttg gaattggaaa agtcccagct atcgttgctt
     1501 tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg aataatgtca
     1561 atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa tctatatttc
     1621 aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg tctactattt
     1681 atgttattgc ttgggctaca ttagcttctt atataggtgc aggtggactt ggtgatctca
     1741 tttttagcgg gttaaatctt tttcagccca aattaattct agggggcaca attcctgtta
     1801 ttatcctgtc acttattata gattatcttt taggattact ggaaacagct ctaacgccca
     1861 gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac agatatttac
     1921 actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta aaaaacctgg
     1981 tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatca tggcaaatat
     2041 tgtcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa gcaatcttgg
     2101 ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg ctgccacacg
     2161 ttatacagga acagacatca caggaactct tggcttaaaa gctgttaaag accctaaaga
     2221 agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt ggtatcctac
     2281 ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca gacagaataa
     2341 aatcaccaag atctctgatc tcaaaaagtt atcaacaact atgaaggcag gggttgatag
     2401 ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat acggttttga
     2461 attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg aaagtaacaa
     2521 aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg atttagaaat
     2581 tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg tcaacaattc
     2641 tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg gtaaaatcaa
     2701 tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag aaccttcagt
     2761 tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat aaatgaaaca
     2821 aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt atatcttaga
     2881 acaatttatc caccactttt taatttcaat ttacggcgtt attttagctg caattgttgg
     2941 tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg ttatgggaat
     3001 cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta tgttaggtct
     3061 agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc tgccaatcat
     3121 cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg ctgctaaagg
     3181 aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat ctatttctgt
     3241 tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag ccattggtgc
     3301 tttcgtcggt ggaggcggac ttggagatat catcataaga ggaactaatg caacaaatgg
     3361 tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt tctctgattt
     3421 aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa aaacttgtca
     3481 gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa ttgtttttta
     3541 ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac aataaagcta
     3601 acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg cataaacata
     3661 atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc gaccctgatt
     3721 tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa aagaaaacta
     3781 ccactgtaag atatccatat cggtaattca aatagagatg ggaatcccat aactatgact
     3841 cccaaactac tgaatactag catagatagc atagaat
//
LOCUS       DATRNX010000138         3858 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRNX010000138 DATRNX010000000
VERSION     DATRNX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            99..1328
                     /locus_tag="VBC81_001851"
     CDS             99..1328
                     /locus_tag="VBC81_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6912856.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            1426..2199
                     /locus_tag="VBC81_001852"
     CDS             1426..2199
                     /locus_tag="VBC81_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6912857.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
     gene            2408..3769
                     /gene="dnaA"
                     /locus_tag="VBC81_001853"
     CDS             2408..3769
                     /gene="dnaA"
                     /locus_tag="VBC81_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6912858.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1390 a          645 c          674 g         1149 t
ORIGIN      
        1 tatccacaac ttgtggatat ttcctttttt tagcttttaa ggtataatta agaagcttgt
       61 cataagatta gtataaaaat ttacggaggt agctatttgt gaaaaaaaaa ttagtctcat
      121 cacttctaaa gtgttctcta atcattattg ttagctttgc tggtggagca tttgctagtt
      181 ttgtcatgaa tcataatgac aatattccaa atggtggtgt cactaaaact agtaaagtaa
      241 attataataa cataacgcct acaacaaaag ctgttaaaaa ggtacaaaat agtgttgttt
      301 ctgttatcaa ttataaacaa caagagagtc gttctgacct atcagacttc tatagtcatt
      361 tttttggtaa tcaggggggc aacactgata agggcttaca agtttacggt gaaggctctg
      421 gagtcatcta taaaaaagat ggtaaaaatg cctatgttgt cactaataac cacgtcattg
      481 atggggctaa acaaattgaa attcaactag ctgatggctc aaaagcagtt gggaaacttg
      541 ttgggtcaga tacctactct gatttagccg tcgtcaaaat tccatcagat aaagtttcaa
      601 atattgcaga atttgctgat tcatcaaaac tcaacattgg tgaaactgct atagcgatcg
      661 gaagccctct tggaactgag tatgcaaatt ctgtaactca aggtattgta tctagtttaa
      721 aaagaactgt aacaatgact aatgaagaag gacaaacagt ttctacaaat gctatccaga
      781 cggatgctgc tatcaatcct ggtaattcag gtggagcact tatcaatatt gaaggacagg
      841 ttattggaat taattctagt aaaatttctt ctacatcaaa tcaaacctca ggacaatcgt
      901 caggaaatag cgttgaaggt atgggatttg ccattccttc aaatgatgtt gttaagatta
      961 tcaatcaact tgagagtaac ggacaagtag agagacctgc tctaggtatt tctatggctg
     1021 gattaagtaa tttaccatcc gatgttatta gtaaactgaa aatcccaagt aatgttacta
     1081 atggtattgt agtagcatct atccaatctg gcatgccagc tcaaggcaaa ctaaagaaat
     1141 acgatgtcat tactaaagtt gacgataaag aagtagcatc tccaagtgat ttacaaagtt
     1201 tactctatgg ccaccaggta ggggattcca taacagtaac cttttatcgt ggtgaaaata
     1261 aacaaacagt cactataaaa cttactaaaa ctagtaaaga tttagctaaa caacgagcaa
     1321 ataactaaat caattctagt tattttaact aagatatagt aaaattaaag gggttggtca
     1381 gatagaccaa tccctttagt aagtctatag aatctgaggt ctaaaatgga atatttagaa
     1441 acgatcaaca tcaatcatat tgctccaaat ccgtatcaac ctcgcctaga atttaataca
     1501 aaagaactag aagaattagc cgactctatt aaaattaatg gccttattca acccattatt
     1561 gtaagaccgt cagcagtctt tggttacgaa ctggtagccg gagaacgacg gttaagagcc
     1621 gcaaagttag ctaaattgga aagtatacct gcaattataa aatcctataa taatgatgat
     1681 agtatgcaat tagctattgt cgaaaattta caacgatcta atttgtcacc cattgaagag
     1741 gctaaagcct actctcaact tctacaaaag aaaagtatga cacatgaaga gttagcaaaa
     1801 tatatgggaa aatctcgtcc ctatatatca aatacgatac gactactaaa tttaccgcca
     1861 ctaattactt cagctattga agaaggaaaa ctttcctctg gtcatgcgcg tgcactactc
     1921 agtctaccag atgctagtca acaaaaagat tggtatcaga gaatcctcac tgaagatatt
     1981 agtgtaagac gactagagaa gttattaaaa caagaaaaga aaacaaatca taagtcactt
     2041 caaaataaag atgtctttct aaaacatcaa gaaaatgagt tagctcaatt tctaggtagc
     2101 aaagtgaaac ttactattaa taaagatggt gctggcaata taaagattgc tttcaataac
     2161 caagaagaat taaacagaat tatcaacact ctaaaataag ggtgttgatt atttttttat
     2221 ttaatcaaac ttatccacaa ggtattttgc tatttttctg ttgattctct aagcttttct
     2281 aatttccaca gtctgtggaa aactttaatt aacattgttg attttattct tcaacatctg
     2341 tggaaaactt tattttttta tggtacaata taacaataat tatccacaag acaataagga
     2401 agaagctatg acggaaaacg aacaactatt ttggaataga gtactagagc tatctcgttc
     2461 tcaaatagca ccagcagctt atgaattttt tgttctagag gctagactcc tcaaaattga
     2521 acatcaaact gcagttatta ctttagataa cattgaaatg aaaaagctat tctgggaaca
     2581 aaatttgggg cctgttatcc taacagctgg ttttgaaatt ttcaatgctg aaattacagc
     2641 taactatgtc tcaaacgatt tacatttaca agaaactagt ttttctaact accagcaatc
     2701 tagcaacgaa gtaaatactt taccaattag aaaaatcgac tctaacctta aagagaaata
     2761 tacttttgct aattttgttc aaggagatga aaatagatgg gctgtttcag catcaattgc
     2821 tgtagctgat agtcctggca cgacttataa tcctctattt atctgggggg gacctggtct
     2881 aggaaagacg catctactaa atgctattgg aaatcaagtt ttaagagata atccaaacgc
     2941 gagggtttta tacatcactg ctgagaattt tattaatgaa tttgtcagtc atattcgttt
     3001 agattcgatg gaagaattaa aagaaaagtt tcgcaacttg gacttactcc tgattgatga
     3061 tattcagtcg cttgctaaga aaaccttagg ggggacccaa gaggagttct tcaatacttt
     3121 caatgcttta catacaaacg ataaacaaat cgtattgacc agtgaccgaa atccaaatca
     3181 attaaatgat ctagaagaac gtctagtcac gcgctttagt tggggactcc cagtaaatat
     3241 cacaccacct gattttgaaa cacgagttgc tattttaacc aataaaattc aagaatatcc
     3301 ttatgatttt cctcaagata ccattgaata cttagcagga gaatttgatt ccaacgtacg
     3361 tgaattagaa ggagccttga aaaatattag tctagttgct gactttaagc atgctaaaac
     3421 tattacagta gatatagctg cagaagctat cagagcacgt aaaaatgacg gtcctattgt
     3481 tactgttatt cctatagaag aaattcaaat acaagttggt aaattctatg gcgtaactgt
     3541 aaaagagata aaagcaacta aaagaacaca agatattgtc cttgcaagac aggtagccat
     3601 gtacttagct cgtgagatga cagataacag tctccccaaa ataggtaaag aatttggggg
     3661 acgagatcac tcaactgttc tccacgctta taataaaata aaaaatatgg ttgctcaaga
     3721 tgacaactta cgaattgaga tagaaactat caaaaataaa atcaggtagg ctttgtggat
     3781 aagtatcaga aaaacacgag acttttccac aggttgtgaa caagttattt tccttatgaa
     3841 tactaaagtt ataatact
//
LOCUS       DATRNX010000139         3809 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRNX010000139 DATRNX010000000
VERSION     DATRNX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..521
                     /locus_tag="VBC81_001854"
     CDS             <1..521
                     /locus_tag="VBC81_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO6912859.1"
                     /translation="INPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            532..1221
                     /locus_tag="VBC81_001855"
     CDS             532..1221
                     /locus_tag="VBC81_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6912860.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
     gene            1234..2622
                     /locus_tag="VBC81_001856"
     CDS             1234..2622
                     /locus_tag="VBC81_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6912861.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFIFKNSFTTTRLSF
                     FTFIAMNSILLYLLNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYI
                     AVCILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDII
                     NDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSVITYSMKFYKYSHLIAKRFLDITG
                     AIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGRNGRIFRFYKFRSMRVDAEQIKKD
                     LLVHNQMTGLMFKLEDDPRITKIGKFIRKTSIDELPQFYNVLKGDMSLVGTRPPTVDE
                     YEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDEIVKLDVQYINEWSIWSDIKIILL
                     TLKVVLLGTGAK"
     gene            2646..3095
                     /locus_tag="VBC81_001857"
     CDS             2646..3095
                     /locus_tag="VBC81_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6912862.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            3095..3568
                     /locus_tag="VBC81_001858"
     CDS             3095..3568
                     /locus_tag="VBC81_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6912863.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
BASE COUNT         1318 a          521 c          696 g         1274 t
ORIGIN      
        1 tcattaaccc taatacccct aataatagta ttactgcaca agatctacaa gcggggagtt
       61 ttcttgcaaa tgactataag gagattatta cgtctactga cgttctagaa aaagttattt
      121 cttctgaaaa attgaattat ccttcgtctc agttgctaca aaaaataaca gtttctattt
      181 taaaagatac acgtgttatt tcaatatcgg tcgaagatgc taatccaaaa atgtctcaaa
      241 aattagcaaa ttcagttaga gaagcagcag tttcaaaaat caaggcagtt actcaagtag
      301 aggatatcac tactcttgag aagggaaatt tacctaaagc accatcttct cctaatatta
      361 aaaagaatgt actaatcggg tttattgttg gtgcaggatt atcaactatt gttttagtta
      421 ttatggggat tttggatgat cgtgttaata ctgaagaaga tattgaaaag gtcttaggac
      481 ttacttcttt aggtatagta ccagatttga ataaacttta aggagaatat aatgactcgt
      541 ttagaaatag ttgatagcaa gttaagacaa gcaaaaaaaa cagaagaata cttcaatgcg
      601 atccgtacaa acatacagtt tagtggaaag gaaaataaaa ttcttgcaat tgcctctgtt
      661 agggaagggg aaggaaaatc cactacttca acaagtttag ctttatcttt agctcaagca
      721 ggatttaaaa cattattaat tgatgcggat actaggaact ctgttatgtc tggaaccttt
      781 aaagcaactg gaactattaa aggcttgacg aattatttat caggtaatgc agatcttgga
      841 gatattatct gtgaaaccaa tgttcctaga ctgatggtcg ttccttcagg aaaagtacca
      901 ccaaatccaa cagcattact tcagaacgct tattttaata agatgattga agctattaaa
      961 aatatatttg attatattat catcgatact ccacctattg gtttagttgt tgatgccgca
     1021 ataatcgcta atgcttgcga tggttttatt ttagtaaccc aagcaggcag aataaaacgt
     1081 aattatgttg aaaaagcaaa agaacagatg gaacaaagtg gttcaaagtt cttaggtatt
     1141 attcttaata aagttaatga atctgttgct acttacggcg attatggaaa ttacggaaaa
     1201 agggatagaa aaaggaagta agggactctg gtattgaaag aaaaagaaaa tatacaaaag
     1261 attattatag cgatgattca aacagttgta gtttattttt ctgcaagttt gacattaaca
     1321 ttaattactc ccaattttaa aagcaataaa gatttattgt ttgttctatt gatacattat
     1381 attgtttttt atctttctga tttttacaga gacttttgga gtcgtggcta tcttgaagag
     1441 tttaaaatgg tattgaaata cagcttttac tatattttca tatcaagttc attatttttt
     1501 atttttaaaa actcttttac aacgacacga ctttcctttt ttacttttat tgctatgaat
     1561 tcgattttat tatatctatt gaattcattt ttaaaatatt atcgaaaata ttcttacgct
     1621 aagttttcac gagataccaa agttgttttg ataacgaata aggattcttt atcaaaaatg
     1681 acctttagga ataaatacga ccataattat atcgctgtct gtatcttgga ctcctctgaa
     1741 aaggattgtt atgatttgaa acataactcg ttaaggataa taaacaaaga tgctcttact
     1801 tcagagttaa cctgcttaac tgttgatcaa gcttttatta acatacccat tgaattattt
     1861 ggtaaatacc aaatacaaga tattattaat gacattgaag caatgggagt gattgtcaat
     1921 gttaatgtag aggcacttag ctttgataat ataggagaaa agcgaatcca aacttttgaa
     1981 ggatatagtg ttattacata ttctatgaaa ttctataaat atagtcacct tatagcaaaa
     2041 cgatttttgg atatcacggg tgctattata ggtttgctca tatgtggcat tgtggcaatt
     2101 tttctagttc cgcaaatcag aaaagatggt ggaccggcta tcttttctca aaatagagta
     2161 ggtcgtaatg gtaggatttt tagattctat aaattcagat caatgcgagt agatgcagaa
     2221 caaattaaga aagatttatt agttcacaat caaatgacag ggctaatgtt taagttagaa
     2281 gatgatccta gaattactaa aataggaaaa tttattcgaa aaacaagcat agatgagttg
     2341 cctcaattct ataatgtttt aaaaggtgat atgagtttag taggaacacg ccctcccaca
     2401 gttgatgaat atgaaaagta taattcaacg cagaagcgac gccttagttt taagccagga
     2461 atcactggtt tgtggcaaat atctggtaga aataatatta ctgattttga tgaaatcgta
     2521 aagttagatg ttcaatatat caatgaatgg tctatttggt cagatattaa gattattctc
     2581 ctaacactaa aggtagtttt actcgggaca ggagctaagt aaaggtaagg tttgaaagga
     2641 atataatgaa aatttgtctg gttggttcaa gtggtggtca tctagcacac ttgaaccttt
     2701 tgaaacccat ttgggaaaaa gaagataggt tttgggtaac ctttgataaa gaagatgcta
     2761 ggagtattct aagagaagag attgtatatc attgcttctt tccaacaaac cgtaatgtca
     2821 aaaacttggt aaaaaatact attctagctt ttaaggtcct tagaaaagaa agaccagatg
     2881 ttatcatatc atctggtgcc gctgtagcag taccattctt ttatattggt aagttatttg
     2941 gttgtaagac cgtttatata gaggttttcg acaggataga taaaccaact ttgacaggaa
     3001 aattagtgta tcctgtaaca gataaattta ttgttcagtg ggaagaaatg aaaaaagttt
     3061 atcctaaggc aattaattta ggaggaattt tttaatgatt tttgtcacag tggggacaca
     3121 tgaacagcag ttcaaccgtc ttattaaaga agttgataga ttaaaaggga caggtgctat
     3181 tgatcaagaa gtgttcattc aaacgggtta ctcagacttc gaacctcaga attgtcagtg
     3241 gtcaaaattt ctctcatatg atgatatgaa ctcttacatg aaagaagctg agattgttat
     3301 cacacatggc ggcccagcga cgtttatgtc agttatttct ttagggaaat taccagttgt
     3361 tgttcctagg agaaagcagt ttggtgaaca tatcaatgat catcaaatac aatttttaaa
     3421 aaaaattgcc cacctgtatc ccttggcttg gattgaagat gtagatggac ttgcggaagc
     3481 gttgaaaagg aatatagcta cagaaaaata tcagggaaat aatgatatgt tttgtcataa
     3541 attagaaaaa attataggtg aaatatgagg aaatatctag atttagatta ttctttattt
     3601 tatgctcttt gggtacttat tttagtacca aaccaatggt atcagttttt aattattacc
     3661 attatagttc tattattact ttggaagagt gagtttagaa tatctataag caattcttca
     3721 atactatttc tgctttggtt atttatttat ttatttgcaa tactcattag aggtactcaa
     3781 gaggatataa cgtttcagcg atttattgc
//
LOCUS       DATRNX010000140         3362 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRNX010000140 DATRNX010000000
VERSION     DATRNX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            112..1122
                     /gene="manX"
                     /locus_tag="VBC81_001859"
     CDS             112..1122
                     /gene="manX"
                     /locus_tag="VBC81_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6912864.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            1155..1967
                     /locus_tag="VBC81_001860"
     CDS             1155..1967
                     /locus_tag="VBC81_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6912865.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            1982..2893
                     /locus_tag="VBC81_001861"
     CDS             1982..2893
                     /locus_tag="VBC81_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6912866.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            3012..>3362
                     /locus_tag="VBC81_001862"
     CDS             3012..>3362
                     /locus_tag="VBC81_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6912867.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFK"
BASE COUNT          997 a          638 c          676 g         1051 t
ORIGIN      
        1 tatctgttct ataatttcgt tgaaatgaaa aagaatggcc aattttacga ttggaaggag
       61 attattcttc tttatgtcaa cgaaaaaatt ttttaaggag gaagaacaag aatgggtatc
      121 ggtattatta ttgccagcca tggtaaattt gctgaaggta ttcatcaatc aggttcaatg
      181 atctttggtg aacaagaaaa agttcaagtt gtaactttca tgccaaatga aggaccagat
      241 gatttgtatg gacacttcaa caatgctatc gcacaatttg atgctgatga tgaggtgctt
      301 gtgttagctg acctttggag tgggtcacca tttaaccaag caagccgtgt tatgggagaa
      361 aatcctgaac gtaaaatggc tatcatcact ggtctcaatt tgccaatgct tatccaagcc
      421 tatactgagc gtatgatgga cgctaatgca ggtgttgaac aagttgccgc aaatattatt
      481 aaagagtcta aggaaggtat caaagccctt ccagaagaac ttaatcctgt tgtagaagct
      541 actcctgtag caggtgttcc tgcagatgtt cctgcggaag ttaaacaaag tggatctatc
      601 cctgaaggaa ctgttattgg agatggcaag cttaaaatta atcttgctcg tattgacaca
      661 cgtcttttac atggtcaagt tgcaactgca tggactccag catcaaaagc aaaccgtatt
      721 atcgttgctt ctgatgaagt ttctaaagat gaacttcgta aacaattgat taagcaagct
      781 gctcctggag gagtcaaagc aaacgttgtc ccaattagta aattaattga agtagctaaa
      841 gacccacgtt tcggaaacac acgtgccttg atcttatttg aaactgtaca agatgcactc
      901 cgtgcagttg aaggtggtgt ggaaatccct gaattgaacg ttggttcaat ggctcactca
      961 actggtaaaa caatggtaaa caacgttctt tcaatggata aagacgatgt tgctgccttt
     1021 gaaaaattac gtgacttagg tgtatcattt gatgtacgta aagtaccaaa tgacgctaaa
     1081 aagaatttgt ttgatttaat caacaaagca aacgtaaaat aacttattat ctctgaaaac
     1141 gaaaggatat caaaatggcg gatatttcaa ttatctcaat gattttggtc gttatcgttg
     1201 ccttctttgc tggtcttgaa ggtatcctcg accaattcca aatccatcaa cctcttgttg
     1261 cttgtacttt aatcggtctt gtaactggtc acctcgaagc gggtattatt cttggtggta
     1321 gtctccaaat gattgctctt gcttgggcaa atatcggagc tgccgtagca ccagacgctg
     1381 cacttgcctc tgttgctgct gctattattt tggttaaaag tggcgacttc actcaaaaag
     1441 gtatcaactt tgccttctct actgctgtac ctctagctat tgctggactt ttccttacaa
     1501 tgattgttcg tacaatctca actgcacttg ttcacgctgg tgacaaagct gcttctgaag
     1561 gaaacttcgc agcaattgaa cgttttcact ttattgccct ccttctccaa ggattacgta
     1621 ttgcattccc agctgcgctt cttttagcta ttccatcttc ttcagtacaa agtattcttg
     1681 aagcaatgcc tgattggtta aatggtggta tgcaagttgg tggtgccatg gttgttgccg
     1741 ttggttacgc tatggttatc aatatgatgg ctactcgcga agtttggcct ttctttgctc
     1801 ttggatttgc tctagcagct ttgaaccagt taactcttat tgcaatgggt actattggtg
     1861 ttgctatcgc cctcatctac atcagtttat caaaaatggg tggaagcaaa ggtacttcta
     1921 acgcaggttc taacgatcct atcggcgata tcttagagga ctactagaaa ggagctaaac
     1981 aatgactgaa caaattaaat tatcaaaatc agatcgtcaa aaagtttggt ggcgttcaca
     2041 attccttcaa ggttcttgga actatgagcg tatgcaaaat atggggtggg cctacgctct
     2101 tatcccagca ttgaaaaaac tttacactac taaagaagat cgtgctgctg cgcttgagcg
     2161 ccatatggaa ttctttaaca cacatccata cgttgctgct ccaattatcg gtgtaaccct
     2221 tgctcttgag gaagaaaaag caagcggtac acctgtcgaa gacaaagcaa ttcaaggggt
     2281 taaaatcggt atgatgggac ctcttgctgg tattggtgat ccagtattct ggtttactgt
     2341 acgtcctatc ttaggtgctc ttggtgcttc acttgcctct gctggtaata tcctcggacc
     2401 aatcatcttc tttgttggtt ggaacctcat tcgtatgtca ttcttatggt atacacaaga
     2461 gttaggttac aaatcaggta aagaaattac taaagatatg tctggcggta tcctccaaga
     2521 tattactaaa ggtgcttcaa tccttggtat gttcattctt gctgtccttg ttaagcgttg
     2581 ggtagcaatt aactttactg tagaccttcc taaaaagaca ttgtcagaag gggcatacat
     2641 caatttccct aaagaccatg ttagtggaca acaattgcat gatatcctag gacaagttca
     2701 gagtggctta agccttgata aaatgcaacc acaaacactt caaggacaac ttgactcact
     2761 tattcctggt ttagctggat tacttcttac attcttctgt atgtggttac ttaagaagaa
     2821 agtatcacca attacaatta tcatcggttt gtttatcgtt ggtattctag ctcgccttgc
     2881 aggtgtgatg taataaaact aagagatggt tacaatagta ttccatctct tatattttat
     2941 taccaaaaat ccacttagag tcccactatt atgatataat caaactacta taaattaaaa
     3001 agggattagt catggcacaa tcattaaaca aaactgttga atttcaaaca acaggagttt
     3061 cgtatttagg tatggggaat aaagttggaa aatttttagt tggtgaccag gcattagaat
     3121 tctataacga taaaaatgtc aacgactata ttcaaattcc atggacatca atcaatcaaa
     3181 ttggagctaa tgttagtcga aaaaaaatta gtcgtcactt tgaggtcttt acagaccaag
     3241 gaaaattttt atttgcctct aaagactctg gaacgatatt aaagcatgcg agacgacata
     3301 ttggagacga taaagttgtt aagcttccta ccctcatcca gaccatttta aaaatattca
     3361 ag
//
LOCUS       DATRNX010000141         3215 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRNX010000141 DATRNX010000000
VERSION     DATRNX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1258)
                     /locus_tag="VBC81_001863"
     CDS             complement(<1..1258)
                     /locus_tag="VBC81_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6912868.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGL"
     gene            complement(1258..1839)
                     /locus_tag="VBC81_001864"
     CDS             complement(1258..1839)
                     /locus_tag="VBC81_001864"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6912869.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAEAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(1943..2038)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2094..3077)
                     /gene="guaC"
                     /locus_tag="VBC81_001865"
     CDS             complement(2094..3077)
                     /gene="guaC"
                     /locus_tag="VBC81_001865"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6912870.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
BASE COUNT         1105 a          660 c          477 g          973 t
ORIGIN      
        1 gcaatccatt caaaatgata tttaaaacaa cagctgtcag ggttgagata acaataccat
       61 ttgtcaaaaa catttgtaaa gtattaggaa gactaatgaa gagatttgtc tcattaaaac
      121 ctactcctgc tgcaattgat acggctgcaa tgataaagtt atgctcatta tgctcaaaat
      181 caacctgatt aagcattttc atcccttgaa gtgctaccat accaaataaa actaacatag
      241 caccaccaag aactggactt ggaatcattt gagccattgc gccaaattta ggtaacaagc
      301 caagtataac taagaagaga gctgtaaaat atatcggctt acgcgtacgt atcccagata
      361 tctgtaccag tcccacattt tgagaaaaac cagtatatgg aaaggtatta aataagccgc
      421 taagtaatac tgccaatcct tctgaacggt aaccattacg aagtctctta ctgtctaatt
      481 tatcgtttgt aatatctgaa agcgcaaggt aaacacctgt tgattctacc atagaaactg
      541 ttgcaataat acacatcatt aaaatagaag taaattcaaa tctaggagct ccaaaataaa
      601 atggcttcgg aatatgtaca agtggtgctt ctgctaccac agaagcatca actaagccca
      661 taaatgctgc aagaattgta cctgaaatga gtccaataag gattgaaatt gacttcaaaa
      721 agccttttgc gaaaatatta attaataaaa caacaccaat cgttaccagt gataaagtta
      781 aagattgtaa gcttggttct tttgcattat cacccatatt tcccatcgca actggtatta
      841 atgtcaaacc aattgtagta ataactgatc ctgttacaat tggaggaaag aaatttgcaa
      901 ctttagaaaa aatgccagca actaaaacga catatattcc tgaagcaatt aaagctccaa
      961 acatatagcc agaaccttgt tgtgcaccaa taattgataa aggagctaca gactgaaagg
     1021 cacaccctaa tacaactgga agacccactc caaagtgctt acttaatcgt aattgtaata
     1081 aggtggcaat cccacacata aaaatatctg tcgcaataag ataagttagt tgcttagcat
     1141 tataaccaag agcactagca atcatgatag gtacaagaat ggatcctgca tacatagcta
     1201 acaggtgttg caagcctaga agtgctgctt gcgaattact ttgctcatta aatttcatta
     1261 agcatcagcc tccgcaaaaa caactcggcc attttcaaac gctttaatac gagcaagaga
     1321 agtaaccgga acacctgttt tttctaacaa atcacgccca tcttggaaag atttttcaat
     1381 aacgattccg ataccagcaa ccttagcttc agcttgacca ataatttcaa gtaatccttt
     1441 agccgcttga ccgtttgcta aaaagtcatc aatgatgagt acagtatcat cgttagataa
     1501 aaagcgactc acaatagaaa cttgactcgt aacttgcttt gtaaaagaat acacttcagc
     1561 agttaagata ccttcagtca tagtaatgtt cttagctttt ttagcaaata tcattggtac
     1621 gcccaatgct tgagctgcgt acactgctgg cgcaattcca gatgcttcaa ttgtaacaac
     1681 cttcgtaatg ccggcttctt tatatttatc agcaaaaact ttacctattt cctgcattaa
     1741 ctcaaaattt acctgatgag tcaaaaaaga atcaactttt aaaatgttct cacctaaaac
     1801 atcaccatct ttaagaatac gttcttccaa taacttcata ataacccctt cactctttag
     1861 aaaataaaaa aataacttac ctcaatcaga ggacacaaat ctcctctaat ttatcgcttc
     1921 attaacatga accaacaggc aagctattta ttagcttact tattgttaga cattacggtg
     1981 tcttgtagag acgctaagcc aatcctcagc ataaataagt aaatcatata tttaattgtt
     2041 atgtatgata gataactatt gtaacatttt taattaagat ttcaagattt aatttaaatg
     2101 gaatcaccat tccaaataga atttttaaca ataacgtaat caacgtgtct caagctatcc
     2161 aattcttttc cacctgcgta agaaatagaa ctttgcaaat cttgttgcat ttctgtcaaa
     2221 gtatcctcta gtcgcccttt aacaggtaat agaattttct taccttcaac atttttatgt
     2281 tctccctttt gatattcaga agctgagcca tagtattctt taaattgctg tccttctact
     2341 tccacaagtt ttccaggact ttcaaggtga ccagcaaaca gcgaaccaat cattaccata
     2401 ctagcaccaa agcggataga ttttgcaata tccccatgtg ttcgaatacc accatcagca
     2461 ataattggct tgcgagcagc tttagaacac caacgtaagg cagctaattg ccaacctcca
     2521 gtaccaaaac ctgtcttgac tttagtaata caaactttac caggtccaat accaacttta
     2581 gttgcatctg cacctgcatt ttccaattct ctaacagctt caggcgttcc tacatttcca
     2641 gcaataacga aagttcctgg aagctcttgt ttaatgtgtt ggatcatctc tataacactg
     2701 ttagaatggc catgtgcaat atcaatagtg ataaactcag gagcatcttc tttcagactt
     2761 gtcacaaaat catattcata gtctttgacc ccaaccgaaa tcgaagcaat taagccttta
     2821 tcatgcatac gcttgataaa aggtcttctt gcttcttcat taaaacggtg cataatataa
     2881 aaataacctt cacacgctaa tgtttcagct acctcttcat caataatagt ctgcatatta
     2941 gcagggatta ctggaagttt aaaggtatag tttccaagtt tgacacttgt gtcagcttgt
     3001 gagcggctag atataataca cttgtttggt attaattgaa tatcttcata atcaaaaaca
     3061 ggaatatcgt taaacataat ggttctccta atgagtaaaa tgaccttcct ataactttgt
     3121 ataacaaagc atcgctaggt aaaaacatac ctttcctttt taaattatat actttattta
     3181 tttttagtca atttttgatc aatttatttg aataa
//
LOCUS       DATRNX010000142         3206 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRNX010000142 DATRNX010000000
VERSION     DATRNX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(18..860)
                     /locus_tag="VBC81_001866"
     CDS             complement(18..860)
                     /locus_tag="VBC81_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6912871.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            1127..1801
                     /locus_tag="VBC81_001867"
     CDS             1127..1801
                     /locus_tag="VBC81_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6912872.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            1889..2281
                     /gene="gloA"
                     /locus_tag="VBC81_001868"
     CDS             1889..2281
                     /gene="gloA"
                     /locus_tag="VBC81_001868"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6912873.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            2410..>3206
                     /locus_tag="VBC81_001869"
     CDS             2410..>3206
                     /locus_tag="VBC81_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6912874.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWA"
BASE COUNT         1009 a          556 c          553 g         1088 t
ORIGIN      
        1 atttccatct tttcttatta aaatgagaca ttatctggat ctttgggttt aatacctgaa
       61 tctagttgta ttaaggtagc aatatcatct tcgtttaatt gaaaatcaaa aatatcaagg
      121 tttgcttcaa tatttttggg agtcgctgac ttaggtaatg gtaaaaagcc attatcaagt
      181 gaccagcgaa gagctacttg tgcaactgat ttaccatatt tttcagcaat agcttttata
      241 gattcatttt caaaaatagt acctgttcca aatggactgt aagcttctaa taagatatca
      301 tttcctttac agaagcgaac caaatcttct tgagcacaac ctggtgctag gataatttga
      361 ttaaccatag gttttatttc ggcggtttca aataaagcct ctaagtgatg tttcatgaaa
      421 ttcgaaacac caatagcttt tacctttcct tctttataag cttcttccat cgctttccaa
      481 gtgcctgcat tcccagcttt ccacgcatca ttttcacgaa gagcttttgg atttggccaa
      541 tgaatcaaaa gaagatcaat ataatcaaca cctaatttct gcagagattc atcaatagaa
      601 gctttggcta aatgatagtc atgtttgtca ttccaaatct tagttgtcaa aaagatactt
      661 tctcgcgcca aaccactgtc tcttatagca cgtccaacac tatgttcatt accataaatc
      721 tgagcagtat caatatgagt ataaccaact tttaaagcat gagtcactgc tttataagct
      781 tcttcacctt cagttagttg ccatgtccca aaaccaattt taggaatgtt aagagtattc
      841 gatagagtat atgtttccat aacaatctcc ttttctatgt gacaaaaatg aatttcatag
      901 tgaaaaacat ctattttaaa catctaaatg atagtttgat atatggtatt atcccctcct
      961 tatgtaaaat tatataactt tcttcatcaa aaccctatca ttttgttcat tccctttatt
     1021 tttctgacag tcattagaat tttgtagctc tactttgcca cgataccgcc tgctgagatt
     1081 ttttctaaat tgtgatatga tagcattaat aacaggagga tctaaaatga caaaagaaga
     1141 tatcaaaaaa caagttcgaa gagcttttga ttttcgtatg gcgattcgtg tttataataa
     1201 taatgatatt cctaaagaag atatggagta tattttagac actgcttggt tgagtccttc
     1261 ttcagtcggt ttagaaggct ggcgattttt agtactagat agacaaacaa ttgcaaaatt
     1321 tcgagataaa cttaaggaag ttgcttgggg agcacaatat caactagata ctgctagtca
     1381 ctttgtttta ctactagctg aaaaaggtgc ttattataac gctgatagca tgattaacag
     1441 tctcattcga cgtggtctag gtgatccagc cgcccttgag agtaggatac ctctctacaa
     1501 atccttccaa gaaaacgata tgaaaattga ttctgaacgt tctctttggg attggacagc
     1561 aaaacaaact tatatcgcac ttggtaatat gatgacggct gctgctatga tcggcgtaga
     1621 ttcatgccca attgaaggat ttgactatga aaaggtcaat aatattctta gtaaagaagg
     1681 gttaatagac gataagaaag aagctatttc ttgtatggtt tcatttggtt atcgtttgcg
     1741 tgaacctaaa cattcccgtg ctaggaaaga acgtcaagag gtcattactt gggttgagta
     1801 aatagacagc ctaatttata atcaatattt ttgaaaagat aattttattt tgacataata
     1861 cgtttaataa agaaatagga gagagactat gccattttta catacttgta ttcgcgttaa
     1921 agatttggac gcctcaattg ctttttatca agaagcactt ggttttaagg aagttcgtcg
     1981 taatgacttt cctgaaaatc aatttacttt agtctatatg tccctagagg acgaccctag
     2041 ttacgaatta gagttaacct ataactatga tcacgaagct tatgatttag gaaatgggta
     2101 tggtcacatt gctgttggtg tagacgattt ggaaactact tatgatgccc atcagaaagc
     2161 aggttactct gtaacaaaaa tttctggatt accaggtaaa cctaatatgt tctactttat
     2221 tcaagatcca gatggctata aaattgaagt tattcgactc agtcaattta aagctgacta
     2281 ataacatctc atcatattaa gttaaggact gaggcaaaaa cctagtcctt tttatgatta
     2341 ccttataatc tttctatttt ttcccatatt atgttaaaat ttaagcaaga atgatacgga
     2401 gaattacaaa tggctttatt atcagttatt gtcccttgtt ataatgaaca agagactgtc
     2461 tcaacttttt taacagaaat caaaaaagtt gaatccgaaa tggctcgtta cacccatttt
     2521 gaatatattt tcgtaaacga tggttctacc gatcgtacac tcgaacttct taagaaagca
     2581 gctaaacaat ttgacaatgt tcattacctt tcattttcac gccattttgg taaagatgct
     2641 gctttattgg ctggtttaga acatactact ggtgatttta tcactgttat ggatgttgac
     2701 ttgcaagatc cacccacttt attaccagaa atgtacctaa aacttcaaga aggttatgat
     2761 attgtcgcta caaggcgaaa agatcgtaaa ggggagccgt tgatacgctc tctttttgcc
     2821 aagctttttt ataaattaat caatcaagtg tcagatacta aaatggttga tggtgcgcgt
     2881 gattttcgtc tcatgacaaa acaagttgtt gacagtattc tcgagctaaa tgaagtgaat
     2941 cgcttctcaa aagggatttt ttcctggatt ggatatgatg tcgcttatat tagttatgag
     3001 aatcgagaac gaattgcggg taagacctca tggagtttct ttaatctctt aaaatactct
     3061 ctagacggtt tcatcaactt ctcagaaatt ccattagcta tagctacctg gattggaacc
     3121 cttagttcag tactatcact tttagctatc atttttatta taattcgaaa attactattt
     3181 ggggatcccg tttcaggttg ggcaag
//
LOCUS       DATRNX010000143         3185 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRNX010000143 DATRNX010000000
VERSION     DATRNX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..245)
                     /locus_tag="VBC81_001870"
     CDS             complement(<1..245)
                     /locus_tag="VBC81_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6912875.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKT"
     gene            complement(347..1036)
                     /locus_tag="VBC81_001871"
     CDS             complement(347..1036)
                     /locus_tag="VBC81_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6912876.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(1191..1907)
                     /locus_tag="VBC81_001872"
     CDS             complement(1191..1907)
                     /locus_tag="VBC81_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6912877.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(2333..3037)
                     /locus_tag="VBC81_001873"
     CDS             complement(2333..3037)
                     /locus_tag="VBC81_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912878.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
BASE COUNT          995 a          663 c          430 g         1097 t
ORIGIN      
        1 tctgtttttt taaaagtgac tttatagtta tctgaacctt taaaaacagc tttggctact
       61 tcaatatcaa agccagtcag tttcccatcc ttcacataag aaaaaggtgc cgttgttcca
      121 acagttgcaa aattaacttc ttttttgccc gtcttagtag tatgagattt tgagctacaa
      181 gcagctagag acgctactgt cactaaaata attcctgtta cacttaacac tttttttatt
      241 gtcatctttt ttctcctctt taattgtaca cgacatcata acatctttaa gtttttaact
      301 aatacttatc ttctatcgtc tgagagtttt tgctattata tggatgctac gtttcattaa
      361 caaaataacc aaataaggtt tggttttcaa tttggtgaag cattaattct ggatgccatt
      421 gaacacctat aatacattgt tctgttcgac tttcataagc ttctactgtc ccatctctag
      481 gatctctagc actaactatc agaccttcag ctaacagttt aacactttgt ctatgaagag
      541 aattgatctt aaaatcacct gatataaagc cttctagaaa actccctttt tccacattta
      601 aataatgagc aaatccaatg taaggctctt gatagtgatt atcaatagat tgattaagtg
      661 taccaccaag cgccacattt actaactgca tgccacggca aatcgcaaag attggtttat
      721 tttgtttcaa cgcttcttcg actaatgcaa attcaaaaat atcacgcgca agtgaataat
      781 catcactctc aattatcttc tcttcaccat aataggatgg taaaacattt tggccaccag
      841 atatgatcag cttatctatc atctcaacat aagctttggc actttctgct tcacttattg
      901 gtaggataac tggtaacccg cctgcgtttt taactccttc cgaaatatgc ctagataccg
      961 aatcataata ataacctgga atatctgaca tttctctttc atttcctgta ataccaataa
     1021 taggctttgt taacattttg ctccctcatc aaccaataac tgtctaataa ttatcatttt
     1081 acacctaaaa aaactttttg aataatattt tttacctatc acttctttaa caatacctat
     1141 caactctcac aaaaataaaa accatagtat taatactacg gttttatttt ttagaaatct
     1201 gctacattat gataaacttt ttgaacatca tcatctgatt cgagtgcgtc aataagcttt
     1261 tcaaaagttt caaggtcatc accttccaat actacttctg attgaggaat catttcaagt
     1321 tcagtaactt ggaattcttc tacaccatta tcgcgaagtg cttggatacc tttatgaaga
     1381 tctgttgggg cggtataaac tgttattgtt ccctcttctg cttcaacatc atctacgtct
     1441 acatccgctt caagtaattg ttcgaagaca gtgtcagcat catcaccagc aaaaacgatg
     1501 acaccttttt tatcaaataa gtaggatacc gatcctgaag ctcccatatt gccaccgttc
     1561 ttaccgtaag cagtacgtac atttgctgcc gtacggttaa catttgatgt caaagtatcc
     1621 acaataatca ttgaaccatt tggaccaaaa ccttcatagc gtccctctac gaaagtttca
     1681 tctgtgtttc ctttggcttt atcaatcgct ttatcaataa catgctttgg aacttgtgct
     1741 tgcttagcac ggtccaaaac gaattttaga gctgagtttg actcggggtc tggttcacct
     1801 tgctttgcag caacatatat ttcaacaccg aatttagcgt atacttttga gttagcacca
     1861 tctttagcag tctttttggc aacaatattg gcccacttac gtcccattgg aaaatctcct
     1921 ttgatttcat ttttaataac ctgctggctc atattggcaa gcaatcttaa ttttaactct
     1981 attattatag cactttgata gcaattggta aaatggaaat tctatcacgg tatgcagtag
     2041 aattttttag aattgtcaat tttctattaa ggagaaccta ccgagttcct tttataaaaa
     2101 gactgaacaa attttccttt gcccagcctt gacagattcg gtgttatcct gtatcttacg
     2161 ataaagacta agtaagggaa cgttgtaatt ggacttactc ggtaaagcgt agtggttacc
     2221 ataactgcgc ttcttttatt cgattaattc aatagaattg tagcacacta caaaacgaat
     2281 aacgagcttt ttaaagaaaa atataagaaa agccctttta aaggtgcttt tcttatattt
     2341 gtttcaaatc aatcaatttc tttatttctt taatttcgac atccttaaaa ggaaccgttt
     2401 tttcaaactc gcctctatta acttgtttaa atcctagatc tctgtcatta ccaatttgta
     2461 catcaatagt gcataactca actattttct ctctattttt tccttctcta ataaaaaatt
     2521 gtgtaaatat atggtcacaa tagaagcaac atctttccac ttgatacacc tcactaattt
     2581 cagtttgatc aaccggtcgt atgtgataat attgatattt gctgaaaccc ttttgccact
     2641 ttctactcca taggaaaaat ttatcctgct tactatataa cgacacctcc tttccttgga
     2701 aaactgtctc tcccacatga gtcgcaccat caagtggtgc atctttctca tcaaacgaaa
     2761 tctttttctt agacaaaatc gacattatca actgtgcaga acctattgca taaaaaattg
     2821 ttcctacaat aaatactagt aaaattattg ttagtacaat gattccaaaa actttctgtg
     2881 catccattga ggaataccca gacaaattat ataccaatgt atatccacct acagcagtta
     2941 ttgataggca aataaaaaat aatatcaaca caccgtacga agaatccatg atcttacata
     3001 ctaacttttc tgatagaaat ttccatacaa ggcgcacaac acttccctcc ctaaatatat
     3061 taatataata gctatacaca ttaaagtgac catattcgga tattgatcat ctagtctcat
     3121 gtcatcaaaa atcaaaaact caataaaatt ctatgctaca tggctataat tgaagtaagc
     3181 tgagt
//
LOCUS       DATRNX010000144         3176 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRNX010000144 DATRNX010000000
VERSION     DATRNX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..88
                     /locus_tag="VBC81_001874"
     CDS             <1..88
                     /locus_tag="VBC81_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6912879.1"
                     /translation="YELINNSKEELFNRKVIEKIIRTSIENY"
     gene            98..1084
                     /gene="dhaQ"
                     /locus_tag="VBC81_001875"
     CDS             98..1084
                     /gene="dhaQ"
                     /locus_tag="VBC81_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6912880.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            1339..1614
                     /locus_tag="VBC81_001876"
     CDS             1339..1614
                     /locus_tag="VBC81_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912881.1"
                     /translation="MKKNSLYNQLINSEPIGFIDPLTDLGEFDIFQMKFKEPVRYLKN
                     KYSGKPYSPHWQNKIEKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            1756..2034
                     /locus_tag="VBC81_001877"
     CDS             1756..2034
                     /locus_tag="VBC81_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6912882.1"
                     /translation="MTGTQNLNANFEDEKSSMVYYENMVAHYIRLTNHHVRYRVTLLF
                     KNIEMVTRGVGMETQSVEDNQISFDIYIFNVQSGYNINYLTRYSVKEN"
     gene            2044..2244
                     /locus_tag="VBC81_001878"
     CDS             2044..2244
                     /locus_tag="VBC81_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912883.1"
                     /translation="MNSEIRNRKHVYLNDDIIFQLAEISEFLKSSQSTFKQERSPYSQ
                     IIDLRKEEAQAKSVRNSIEKLL"
     gene            2375..>3176
                     /locus_tag="VBC81_001879"
     CDS             2375..>3176
                     /locus_tag="VBC81_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO6912884.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMF"
BASE COUNT         1217 a          402 c          611 g          946 t
ORIGIN      
        1 atatgagtta ataaataact caaaagaaga attatttaat aggaaagtca ttgaaaaaat
       61 tatcagaaca tcaatagaaa attattagga caaggatatg agaaaaacag aacatcataa
      121 aatggaatat gtttttgaaa caataaaagc aatcactaaa actaatccta aagtagagca
      181 aataaaagat ttgcccgcta ttcattattt agggcaatcc gagcaagtaa ttccgatttt
      241 atcaggagga ggcagcggac atgagccggc tcactttgga tatgttggtg agggaatgct
      301 atcggcagcg attagtggtc ctatttttgt accaccatgt gctagtgata ttctagagac
      361 tattagattt atcaatcgag gcaaaggtgt ctttgttatt atcaaaaatt ttgaagctga
      421 tttggaagaa ttttcccaag cgattgaaca ggctcgtcaa gaaggcattc caatcaaata
      481 catcgtttct catgatgaca tctctgttga aacatcaaat tttaaaattc gtcatcgagg
      541 cgttgctggt actgtgttgc tacataaaat tattggacaa gctgctttag aaggagcaag
      601 cttagatgaa ttagaacagc taggactatc tttgacaact tcaatggcta ctttaggtgt
      661 agcaagtaag tcagccacta ttttaggtca gcatcaacct gtttttgatt tagaagaagg
      721 ttacatctca tttggaatcg gtattcatgg tgagccagga taccggacaa tgcctttcgt
      781 gtccatggaa catttggcaa acgaactagt taacaaattg aaaatgaaat tacgttggca
      841 agatggagaa gcattcattc tattgataaa taatcttgga ggaagttcta aaatggagga
      901 attacttttt acaaatgctg tcatggaatt tctggcttta gacgatttac aattaccttt
      961 tattaaaaca ggccacctaa ttacaagttt agatatggct ggtttatcag ttaccttgtg
     1021 tcgtgtaaag gatagtagat ggattgatta cttaaaacat aagacagatg ctagagcttg
     1081 gtgatagaag taagtggtaa gagtttttag aatagaaaat actttaagga gaacagagaa
     1141 atctgttctc tttttgttcg cttaaagatc atagaaaggt gaagagaaca tggaaggtat
     1201 tgaaataata ttagatatag aaacgttagg aatttttcaa gtcatagatc atggtatctc
     1261 tccagatgat agagaaacat atgaacaatt aactttgttt taaataactt aactttatta
     1321 tcttagagga tgttacatat gaaaaagaat agtttatata atcagttaat aaatagtgaa
     1381 ccgataggct ttattgatcc actaaccgac ttgggggaat ttgatatttt tcaaatgaag
     1441 ttcaaagaac ccgttagata cttgaaaaat aaatattcag gaaagcctta ttcaccacat
     1501 tggcagaata aaattgaaaa aatgaggtct catcaagaaa gcctaagaga agaagttaaa
     1561 gaagagattc cgattactag tagggcaaga aatcaagaaa ataatttgag ttgatcttaa
     1621 attgataaaa agttcgactt ttttcctaaa aaatagtgtg ctataagtca attaaaagat
     1681 gtgctatatt tatttaatag atatcgtctc attgaatatc aactaaccgg agaaaattcg
     1741 aatgcaaaaa atttaatgac aggcactcaa aatttaaatg ctaattttga agatgaaaaa
     1801 tcgtcgatgg tatattatga aaatatggtg gctcattata taagactaac aaaccatcat
     1861 gtgagatacc gtgttacact cttatttaaa aatattgaga tggttacaag aggggttggt
     1921 atggaaaccc aaagtgtgga agataatcag atatcttttg atatttatat ttttaatgtg
     1981 cagtcaggat acaacattaa ttatttaaca agatattccg ttaaggaaaa ttaaaggaga
     2041 aaaatgaatt cagaaattcg aaatagaaaa catgtttatt tgaatgatga tattatcttt
     2101 cagttagctg aaatctcaga atttttaaag tcaagtcagt caacatttaa acaggagcgt
     2161 agtccatatt ctcaaataat agacctacga aaagaagaag cacaagctaa aagtgttagg
     2221 aatagcatag aaaaattatt gtaagaaaac agtgatctaa tacaggtaag gaagtatgag
     2281 ttaggctata aaactaagta aaaaaatatg atataatata aaaaattatc gagtaaaata
     2341 aactggaagc gaaacttaag gagatgatta ttaaatgaaa atatgtattt taggattagg
     2401 cgtaataggc acaacttatg gatatatctt tcaaaaagca ggacatcagg ttgaacattt
     2461 agttagagaa agtaaaaagc aagatgttcc attaaagctg gatataacca tgttagatgg
     2521 gcgatataac aataagggag aagaaaaaac agatatatat actgtgaatc ttgcaaaacc
     2581 aaatacaaat tatgacttta tccttttaag tgttgcaggc gggaggataa aagatgccat
     2641 agcaacactt tcacaaaata atattacagg aagtttgtta ttattttgta acttttggaa
     2701 cagccgtgaa gaaatcgaaa aaatggttgg tgattttaaa tatatcattg gatttccgac
     2761 agcaggtggg aatttgaagc agaaaaagtt gaattgtgtt ctgtttgacc atataatgct
     2821 tgaaagtgaa gaaaaagcaa ctatcacaaa ctataaagag ttgttagaat ttctcgaatc
     2881 tgccgacata aaagtagaaa atccttatga tatgatagag tggatatgga ttcatatggc
     2941 aataaatgca ggtgttactt ctactgcagc aagggcagga aaaattgatg atccaaaaca
     3001 acttgcggtt aatcttatga gagataatca tggattgtct atcgcggtaa aatcaataag
     3061 agaaacaatt aaagtagtaa aaaatagagg ggtcgattta agattatata aaaatgaact
     3121 ttttccttat aaaatacctt ctactattgc aggaataatt atgaaacgaa tgttcg
//
LOCUS       DATRNX010000145         3095 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRNX010000145 DATRNX010000000
VERSION     DATRNX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..668
                     /locus_tag="VBC81_001880"
     CDS             <1..668
                     /locus_tag="VBC81_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6912885.1"
                     /translation="QYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFFEGDYR
                     EKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAITDIEYF
                     SLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLATLLLQ
                     VPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNKNYFKK
                     FIE"
     gene            678..1163
                     /locus_tag="VBC81_001881"
     CDS             678..1163
                     /locus_tag="VBC81_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6912886.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            1320..2801
                     /gene="guaB"
                     /locus_tag="VBC81_001882"
     CDS             1320..2801
                     /gene="guaB"
                     /locus_tag="VBC81_001882"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6912887.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            2877..>3095
                     /locus_tag="VBC81_001883"
     CDS             2877..>3095
                     /locus_tag="VBC81_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912888.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKA"
BASE COUNT         1014 a          480 c          622 g          979 t
ORIGIN      
        1 aacaatattt ttatttcaga caactagatg attttaaaca ctttactatt gaacaatttg
       61 accatattgt gagccacatt aaacatcgta cagctctaaa aaatcatacc ttattttttg
      121 aaggtgatta ccgtgaaaaa ctgtttttaa tccagtcagg acatgtaaaa attgaacaat
      181 cagacgcttc tggttctttt atttatacag attatgttag acaagataca gtttttcctt
      241 atggaggtct ctttttggat gatgattacc atttttcagc agtagctatt acggatattg
      301 agtatttttc tctgccaatg gctttatacg aagaatactc tcttcaaaat atcaatcaaa
      361 tgaagcatct ttgtcggaaa tattctaagc tattacgagt tcatgaaata cgacttcgta
      421 atatggtgac ttcaagtgcc agtatgagag ttatccaatc gcttgcaact ctacttttgc
      481 aagttcctac agaaagggga catttacctt ttcctattac aacgattgaa attgccaata
      541 tgagcggaac tactcgcgag acagttagtc atgttttaaa ggaattacgc caaaaagata
      601 tcgttgaaat gaaaggaaag aagcttctct ataataataa aaattatttc aagaaattca
      661 ttgaataagg agtttatatg aataaaaaag aaacaagaca ccaattaatt cgttccttag
      721 tctctgagac taaggtgcgt acacaacatg agttaaggga attactagaa aaaaatggcg
      781 tttcagtgac gcaagcaaca ttatctcgag atatgaagga acttaattta attaaggtta
      841 acgaaagcag tgataatgct acagaaactt attatgagat tcatagtata tctcagaagc
      901 gttgggaaga acggttacgt ttttacatgg aagatgccct agtcatgctg tttccagttc
      961 aaaaccaaat cgttttgaag acactacctg gcttagctca atcttttggc tccatattag
     1021 attcgatttt attacctgag atcttagcaa ctgtctgtgg tgatgatact tgtttgatta
     1081 tttgtaataa tagtgaagaa gctcaaaagt gttttgagaa attaagtcag tatacgccgc
     1141 ctttcttctt ctcaaaaaaa tagaaaatta agaagatttt ttcattactt taaagagcta
     1201 gaagctatta attctatgct ctttttatat tgacaaatga ttttagcgtg atatgatagt
     1261 aacaatataa ttttaatggg attttttata tctactatct aagaaaagag gattttttga
     1321 tgtcaaattg ggatactaag tttttgaaaa aaggttttac ttttgatgat gttctgctta
     1381 ttcctgctga aagccatgtt ttaccaaatg aagttgatat gaatacaaag ttggctgata
     1441 atttgacatt aaacattcca atcattacag ctgcaatgga tacagttaca gatagtaaga
     1501 tggctattgc tattgcacgt gctggtgggc ttggtatcat tcataaaaat atgtcaatag
     1561 ttgatcaagc agaagaagtt cgtaaagtta aacgctcaga aaatggtgtt attattgacc
     1621 cattcttctt gacaccagat aatactgtct ctgaagctga ggaattgatg caaaactatc
     1681 gcattagtgg agttcctatt gtagagacac tcgagaatcg taaattagtt ggaattatta
     1741 ctaaccgtga tatgcgtttc atttctgatt ataaacaact aatctcagag cacatgacaa
     1801 gtcaaaatct tgtaacagct cctattggta cagatcttga aactgcggag cgtattcttc
     1861 atgagcatcg tattgaaaag ctacctttag tagatgatga gggacgtctt tcaggactta
     1921 ttactattaa ggatatcgaa aaagttattg aatttcctaa ggcagcaaaa gatgaatttg
     1981 gacgtctctt agttgcaggt gcagtaggtg taacctctga tacttttgaa cgtgcagaag
     2041 ctttgtttga agcaggtgca gatgctattg tcattgatac agcacatgga cattcagctg
     2101 gtgtgctccg taaaattgct gaaattcgtg ctcacttccc taatcgcaca ttgattgcgg
     2161 gaaatattgc tacagcagaa ggagcgcgtg cactttatga tgcaggtgtg gatgtcgtta
     2221 aagttggtat tggtccaggt tcaatttgta cgactcgtgt cgttgctggg gtaggtgttc
     2281 ctcagattac agctatttac gatgcagcgg ctgttgcgcg tgagtacggt aagactatca
     2341 ttgctgatgg tggtattaaa tattcaggtg atattgtaaa agcacttgca gcaggtggaa
     2401 atgcagttat gctaggttca atgtttgcag gaacagatga agcgccagga gaaacagaaa
     2461 ttttccaagg gcgtaagttc aagacatacc gtggtatggg ctcaattgca gcaatgaaaa
     2521 aaggttcaag cgatcgttat ttccaaggtt ctgtaaacga ggctaacaaa ttggttccag
     2581 aaggtattga aggtcgtgta gcatacaaag gttcagtagc cgatattgtt ttccaaatgc
     2641 ttggtggcat tcgttctggt atgggatatg taggtgcagc gaacataaaa gaacttcatg
     2701 ataatgctca atttgttgaa atgtcaggag ctggtttgaa agaaagccat ccccatgatg
     2761 ttcaaattac aaatgaggca ccaaattatt cagtacacta aaataaggac aaaaaagcat
     2821 cccctatgag gatgcttttt gttgcataaa agaaacaaat atagtaaagt ttttgtatga
     2881 aaaattatgg aaaaatttta aaagagttga gagaagacaa aggtatatcc ctaagttctc
     2941 tagccaaaag tgctcaatta tcaaaatcaa ctctatcacg ttttgaaaat ggtgagacac
     3001 aaataggtat tgataaattt attaaagctt tacaaacgtt ggaggttggg gtaactatca
     3061 atgaagtatc cattctagat tcaaaagtta aggct
//
LOCUS       DATRNX010000146         3062 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRNX010000146 DATRNX010000000
VERSION     DATRNX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            93..728
                     /locus_tag="VBC81_001884"
     CDS             93..728
                     /locus_tag="VBC81_001884"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6912889.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            748..1611
                     /locus_tag="VBC81_001885"
     CDS             748..1611
                     /locus_tag="VBC81_001885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6912890.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            1641..1967
                     /gene="yabA"
                     /locus_tag="VBC81_001886"
     CDS             1641..1967
                     /gene="yabA"
                     /locus_tag="VBC81_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6912891.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            1967..2836
                     /gene="rsmI"
                     /locus_tag="VBC81_001887"
     CDS             1967..2836
                     /gene="rsmI"
                     /locus_tag="VBC81_001887"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6912892.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
BASE COUNT         1063 a          481 c          593 g          925 t
ORIGIN      
        1 ttaaaaatat cagacatgca caatcaagtt gtgggtgtct ttttttgata aattttgata
       61 gaatagtagt aattataaat aaaggatgta ccatgaaaaa aggactaatg atttcttttg
      121 agggcccaga tggtgcgggt aaaacaaccg ttttagaagc tgttttaccc ttgctcagag
      181 aaaaattatc acaagatatt ctcaccacca gagagcctgg tggcgtaact atttcagaag
      241 aaattcgtca tattatttta gatgttaaac acacgcaaat ggataaaaaa acagagctat
      301 tgctttacat ggctgctcga cgccaacatc tagtggaaaa ggtactccca gctctagaag
      361 aagggaaaat agtcctaatg gatcgtttta ttgatagttc tgttgcctat cagggatcag
      421 gaagaggatt agataagtca catataaaat ggcttaatga ctatgcgact gatagtcata
      481 aacctgattt aactttatat tttgatgttc cttctgaagt aggtttagag cgtattcaaa
      541 aaagtgttca aagggaggta aatcgtctag atctcgagca attagatatg catcaacgcg
      601 tccgtcaagg ttaccttgaa ttggcggatt cggagccaaa ccgcattgtg acgattgatg
      661 cctcccaaca gctagatgaa gtaatagctg agacattctc tattatttta gatcgtatca
      721 accaatgaat caggaagtac aaaggtcatg gatttaaagc gaacacaacc caaattgctg
      781 gaaaagttta atactatttt acagtcagat aggatgagtc atgcctatct tttttcggga
      841 aattttgcaa gtctagatat ggcactttat ttagcacaaa gtcagttctg tgaaaagcgc
      901 cagagtggtt taccatgtca agagtgtaga gcctgtcgtt taatcgctaa tggggaattt
      961 tcagatgtta aaatcataga accacagggg caacttatta agatagagac tataaaagag
     1021 ctgactaaag atttttcaag atcaggtttc gaagggaaat cacaggtctt tatcatcaaa
     1081 tattgcgaaa aaatgcatgt caatgcagcc aacagtcttt tgaaatttat tgaagaacct
     1141 caaagttcat cttatgtcat cttattgact aatgatgaaa acaatgtttt gccaacaatt
     1201 aagagtagaa cgcaaatttt tcgttttcca aaacaattgg acatgttggt tcatcaagct
     1261 gaacaagcag gattgctaaa atcacaagct agcttattag ctcaagtagc agacgatcct
     1321 aaacatttag aaatattgtt gactaacaaa aagttattag attacttgaa tttaagtcaa
     1381 cagtttgtga cgactttggc aaaagatagg caaactgctt atttagaagt atctcgcttg
     1441 acatcgcaag ttgtagataa aaatgatcag gcatttgttt ttcaatggct gacaataatg
     1501 ctggctaaag aaggccaact ttatgattta gaaaatactt atagagctca gcaaatgtgg
     1561 aagagtaatg ttagttttca aaatagttta gagtatatgg tgctttctta gaaaataata
     1621 cgcgcagaga ggaagagtga atggataaaa aagacttgtt tgatgccttt gatgattttt
     1681 cacaaaattt attagttggg ttgtctgaaa ttgagactat gaaaaaacaa atccaaaaat
     1741 tattggagga gaatactgtt ctacgcattg aaaatggtaa acttcgtgag cgtctgagcg
     1801 ttattgaagc agaaacagaa actgcagtta aaaattctaa acaaggaaga gaattactcg
     1861 aaggcattta caatgatggc tttcacattt gtaatacttt ctatggtcaa cgtcgtgaaa
     1921 atgacgaaga atgtgctttt tgtattgaat tattatatag agattgatgg aaatgcaagt
     1981 tcaaaaaagt tttaaatcaa atatacatta cggaacactc tatctagtcc caactccaat
     2041 tggtaatcta gatgatatga cttttcgtgc cattaggatt ttaagagaag ttgattttat
     2101 ttgtgcagag gatacacgaa atacgggact tttactcaag cactttgata ttactactaa
     2161 acaaattagt tttcacgaac acaatgctta cgataaaatc tctgggttaa ttgatttgtt
     2221 aaaagaaggg aaatctttag cccaagtatc tgatgcagga atgccctcta tttctgaccc
     2281 aggacatgac cttgtcaagg ctgctattga aggggatatc ccagttgtat ctataccagg
     2341 agctagcgct ggtattactg ctctcatcgc ttcaggttta gctccacaac ctcatatttt
     2401 ttatggcttc ttaccacgta agaaaggtca acaaataact ttctttgaaa caaagcaaga
     2461 ttaccctgaa acacaaatct tttatgagtc accgtttcga gtctctgata cgctaaaaca
     2521 catgaaagag atttacggag atcgccaagt tgttttagta cgcgaattga cgaaactcta
     2581 tgaagagtat caaagaggaa ccattagtca acttttagag catattgaaa aggtccctct
     2641 caaaggtgaa tgcttaatta ttgttgatgg taagagagat accgagcgag tgaaagacag
     2701 tagccaacaa gatccactag tattagtaaa agaatatatc gctaatggtg ataaaactaa
     2761 tcaagcgata aaaaaagtag caaaagaatt taatctcaat agacaagaac tctatgctag
     2821 tttccatgat ttataagtaa taatataaaa aggttgccaa agaaaaattt ggcaaccttt
     2881 tataatctcc gatttagcta ttggaataaa gaagtaagat acttccaatt tccttaaaat
     2941 ttttaatttc agctcttttg aataccattg caaattggat tttatttctg agagtaggtt
     3001 ataaaagatg gtatgctctt attccggttc tttcaggttt tacaaaacat gagagaactt
     3061 ag
//
LOCUS       DATRNX010000147         3022 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRNX010000147 DATRNX010000000
VERSION     DATRNX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            287..745
                     /locus_tag="VBC81_001888"
     CDS             287..745
                     /locus_tag="VBC81_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912893.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEPKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGIWHIYE"
     gene            809..2443
                     /locus_tag="VBC81_001889"
     CDS             809..2443
                     /locus_tag="VBC81_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6912894.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2462..2734
                     /locus_tag="VBC81_001890"
     CDS             2462..2734
                     /locus_tag="VBC81_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912895.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2744..>3022
                     /locus_tag="VBC81_001891"
     CDS             2744..>3022
                     /locus_tag="VBC81_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912896.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIEL"
BASE COUNT         1151 a          486 c          542 g          843 t
ORIGIN      
        1 gaccttgtct tttttctact agataattta ttgtgaaaag ttacttgtaa tatatattta
       61 tttttttcat tgtagaagaa tttatataat aataaatatt aattattaat ttaattaggg
      121 catttaaaat atacaaatga ttttctgttt acatgttaag actaaaacta taaaaatacg
      181 tgaaaatttt gttatactaa aattaatttt ataatgttgg tgaataatag ttaatttcat
      241 tatatacttg atatataaaa tttatattct aagaaaagag aacatgatga gaaaatatat
      301 aaaatggtta attcccatat caatttttgg gatgatactt ggaggctgtc aaatgaatag
      361 tgaacctaaa agtcagtcaa acgaagtaaa aaatagcaag caatcagaag tgaagaaaga
      421 taaaaaaatg acaaaaaaag aacaattagc ctatctcaaa gagcatgagc aagaaatcat
      481 agattatgta aaattacata acaaccaaat tgagtccgtt caattcgatt ggtcaagtgt
      541 aaaagtagaa caaagcggga atggaactcc acaagggggt gattataatc tttcactgag
      601 aggaaagttt aatcatctac aaaattcaaa attaatagtt gatttttatt tagctcataa
      661 aaatgatatc ccaaatatca aatcaatggg aatgctaaat aagccatata tacataaaaa
      721 tggtatttgg cacatttatg aatagcttga atcatcctat ataaagtaat agaattttta
      781 ccacaagcaa ggtagaggag aagaataatt gagtaattgg aacttagata attttgataa
      841 cttacatagc tctttagcag aaagtgcata taatagtcga cctaattcat ttcctgaatt
      901 atttgagact gactcagtaa cagaagtaaa attttctcaa ccctcagaag ataacaaagg
      961 tcaaatcaca cagggtggca ctaacctccc caatgatggt attgtctacc tgcaaccaga
     1021 taagtcctta aaatctattg atgaaaatgt aaaggtcctc attccagatg tcaatggagg
     1081 ctatcacact gagcattatg ttactcattc ttatcaaaaa ggtgtgctta ctgacgataa
     1141 ggcaggtttt aatgcttact acctgtcaga tactgaaaaa atagatagca ctacaaagca
     1201 cacttatcta gccattcgtg gtagtgatgg gattggtcta gatacactaa acgattgggt
     1261 tagtaacaat gcaatgtttg ctgtctctaa caagtatatt ccacaagcga aattggctaa
     1321 taaggcaatg aaagaaaaaa ttgccgagct aaaaggaaaa gcacctggag ctgttatgga
     1381 tattactggt cactcactcg gtacaattgt atcatctcaa gcagtagtta atcttagcta
     1441 tgcagaattg gagaatgttg gtcaagtagt ccttttcgat ggccctgatg tatcaagaag
     1501 cttggaaaaa atggaaggca tttctgctaa aaaaatccaa gaagctggga agcatgttac
     1561 ctactatgtc aatccctttg atatagttag tatgcttaat cgtgaaaagc catggaaaga
     1621 ccaattcggg aaagtaaatg tcattgttcc tagtaaatat acgggtactt ttgatgagca
     1681 atcttctcac gattttggtg ccttccaaat tgatagtagc ggaaatcctt tagttgcttc
     1741 tgaaagctat catccagaat tattaacatc tggtcaagac ttagctagac tcgaaaaaaa
     1801 attcataaag aatcttttag gagagcatta tgattcggat aagctaggac cagtactcaa
     1861 aggcgctctg actggggacc tgactcgact aattgcaaca ttaaaagctt gtggtatcag
     1921 tgtcactgta aacgaagcta aaaaactgta cgataacttc caaaaagact acaaagccat
     1981 aatttccaaa gctaaaaaag atggcctcga gtgggatcgc aaaaacatcg caagcttcca
     2041 tagtaggatc aaatcagcat caggcagtca acggatttta cttagaactc aaatgctcta
     2101 tatggcaacg caattagcag aagcagatgt ttcagaaaaa gtctctgcaa caaaaaaaca
     2161 tattagtgaa gctaaggata cgattgtaga aatatcgaca tccactattt catcagctga
     2221 aataatggct atgcaccttg gccaatcaga agttgatgct ctggtttcag atataaaaat
     2281 gtcaacagtt tggaatgata gcgtagagac ttctgattat gaagcacttg atcactataa
     2341 aacaaaaatg accacattta cgaccaattt agtaacagtt gcacaaaatt taacagctca
     2401 agatgagcaa ctagctggtg atattgtcac aaacttatcc taaaatagag agattaaatg
     2461 aatgtccaaa gaattgaaat tgcccttgaa taaaatgttg aaagaacaag aaagaatagc
     2521 tattaaggca atagttgcta cagcaaaaag cactattacg gatactggaa caaatttaaa
     2581 agcaaccata gagacagatg cccaagacct aaaaaactat gcatccacct ataacgccaa
     2641 tatcacggat ggtcttgaag gccagtcagc tcaagcagca tctgatttct tgacacaact
     2701 tccaaaacca gagttagata atccagtcaa ctaggaggct agtatggtag atacaaaaaa
     2761 actgcaagaa ctcgatcaag agtatgatca aaatctcaga aacatttatc gtaacagaga
     2821 gcaactagag gatgactttc acttgtttat ggcaagaaca gatagcctaa aagagtctgt
     2881 ctatcaagca actttaggtc aaggttggga attaccccaa gaagctcatg cacacctata
     2941 taatatggat gataataagg atacattcat atcagaattc aatgaatata tggaaaagct
     3001 tgaagaaaag gaaatagagc tt
//
LOCUS       DATRNX010000148         2978 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRNX010000148 DATRNX010000000
VERSION     DATRNX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(320..1105)
                     /locus_tag="VBC81_001892"
     CDS             complement(320..1105)
                     /locus_tag="VBC81_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912897.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            complement(1107..1925)
                     /locus_tag="VBC81_001893"
     CDS             complement(1107..1925)
                     /locus_tag="VBC81_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6912898.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            complement(1928..2920)
                     /locus_tag="VBC81_001894"
                     /pseudo
     CDS             complement(1928..2920)
                     /locus_tag="VBC81_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         1076 a          557 c          452 g          893 t
ORIGIN      
        1 atttcatctt tgtcaacact ttcataaatg acatctgctt tttgcttacc ttttatagac
       61 caaaaaacgc tcttagaaag cacatctaag agcgttttta actttatttt ggtaaagata
      121 tggtaattat tgttctatga gggacattat tctcaacact aatagtccct cttcacctga
      181 tatactcccc ttatagtgga cagtaaaaaa acaaaaattc actagacact ataaggggag
      241 tatatcatca ctaacaaagc taagttagta agcctaaaac ttctaaacac tcaaaaaaat
      301 taatggcttg ccttactaat caagaacccg cactctcata tacttctact cctttatgcc
      361 aaagtattag tgataccata aaagaaatca aggaaatcaa gataacccca ccaatattaa
      421 aatagacatt tctatcttgt aagaagtaag cagcgggata ataagctgta aaagcaaatg
      481 gaatgacaaa actaatgatc caacgcaata gattattata tattgcgaca ggatacttag
      541 caaagtcatt aaacatataa aaaatataga taacagcacc agattgtttg gtccaaaaag
      601 cgatacttga cgtagcaatc ttcaaggagg tataaatcaa agttgcaaag gggataataa
      661 atataaataa taatactttt ggcacgatcc agcttatact agatactgtg gtagataaca
      721 gaataaaacc aaccaataac tcccccaatg catctacttg aaaggtttca actaggacat
      781 gaaataaagg actgattggc ctggttaaat acttatcaaa ttctcctttt cgaataagcc
      841 tttgaccaag agcccacaag ttatcaaaga aaaggtgatc aattcctttt gggaggagag
      901 aaaaaccata aataaaagct atttgctgaa aagtccatcc ttctaaagaa gggatatgtt
      961 gaaaaagaac attcagaaat aaaagattta gcccttgtgt taaaaatact cctaaaacgc
     1021 ctacaaaaaa gtctatttta tactccatga tttgcttcat gtattgttta ataaaaataa
     1081 agtgcattcg ctgatatttt gtcatctcaa cctccttgga tagtgatatg cgactgtact
     1141 tttttccata ttaattgaga taaagctacc attactatta gccaaaatat ttgtaaaagt
     1201 aaggcttgca caatttgact gccatcatat ttaccaataa taatcattac tggtgtgtaa
     1261 attaaagatg aaaatggtaa aaatcctaaa atatcagcaa cgattttcgg aaaaaaagta
     1321 agaggaatca agctccccga cataaatgcg actaacgaat tttttaacag gtttgacccc
     1381 cataaatttt taaaaacaaa tgccgagaag ccaaaacaaa tattaaaaaa gaagttaatc
     1441 aaaaaagcta aaataatgct aataatataa aataccacta acactatagc ttgaatcaaa
     1501 tcagtcccca agaagaggcg tacccccgta ataacaagca aaaacggtac ccctatactt
     1561 aaaaagatga gccatctaga accaatctcc atgaaaaggt aggaagcagc aaagtgaact
     1621 ggacgtaaga gtcgcataat aattgaaccg tcttttactt cgtcacctat cataaatgaa
     1681 ctatcagttc ttgttaataa attagtcaca aaactcataa taatatagag aatcatatct
     1741 gacagctgaa aaccttggat taaggattga cttgaggaat cgaaaactgc tttccataga
     1801 taaaatgcaa caaatgctcc tatcacatca cctatcctat aaaggatgaa atcaacacga
     1861 tatgtgatta atccttggat tcctgtacta ataaaaggtt tataacgtct ccaaattctg
     1921 ttcatcctta tagctcattt ctatagaagc gacgaatgat atcttcaata tccgcatctg
     1981 tcattttaag gtcacgtaca gaaaaatctg ctaatgtctg ctgaattatg tcagcagttt
     2041 ggtagcgaga actatcatac tgaatatcta aaactaagtc attacgttta atattaatct
     2101 ccgactttcc aattaaacta gagctgatat gctcttgtcc tgggcgcaaa tcaaaagaaa
     2161 gagttttcat ctttccaaat gtctctttta gctgagaaac tgtgccatca aatatttctt
     2221 gtcctctatc aatcataaaa atacgatgac aaagttgttc aatatcactg aggtcatgtg
     2281 tcgttagtaa aatcgttgtc tcttcttctt gattaatttg tgtgattgca cgtcggatat
     2341 tatctttcac tgaaacgtcc aagccaatgg tgggttcatc taagaaaaga acctttgggt
     2401 tatgtaataa agaagctgcg atatccgctc tcatacgctg tccgagagac aaagttctaa
     2461 caggatcctt tataaagtca tttagttcca aaacctcatt taaaaaagcc atgcgtttgc
     2521 gaaattcttt atcaggaacg tcatagattt cttttaacac tgtataggtt tcttgtagag
     2581 ctaaatccca ccacaactac gtacgttgcc caaaaacgac tccaatatcc ttaacataat
     2641 tttgtcggtt ttcttgtggc aatttgccat caatacggca aaagcccgaa gttggtttta
     2701 aaatcccagt taacatttta attgttgtag attttcctgc accgttcgct ccaataaatc
     2761 cccaaatttg tcctttaggc acatcaaatg ttaaatcttt aacagcttca aaggtgtgtt
     2821 tttcaggacg aagaaatgat tgaaatgctc cttttaaccc tggtgcttta acggttttta
     2881 taaaattttt ctgaaggtga gaaacttcaa tcattgacat attaatacct cttttactca
     2941 acagtatttt tatagcttaa atcagctcga cagttaga
//
LOCUS       DATRNX010000149         2961 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRNX010000149 DATRNX010000000
VERSION     DATRNX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1030)
                     /locus_tag="VBC81_001895"
     CDS             complement(<1..1030)
                     /locus_tag="VBC81_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6912899.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVS"
     gene            complement(1014..1685)
                     /locus_tag="VBC81_001896"
     CDS             complement(1014..1685)
                     /locus_tag="VBC81_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6912900.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(1716..2402)
                     /locus_tag="VBC81_001897"
     CDS             complement(1716..2402)
                     /locus_tag="VBC81_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912901.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(2402..>2961)
                     /locus_tag="VBC81_001898"
     CDS             complement(2402..>2961)
                     /locus_tag="VBC81_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912902.1"
                     /translation="AFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQLEAV
                     SKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAIGTK
                     MSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFLVIA
                     MISSALSIVKVAKIDPVEVINGGGE"
BASE COUNT          951 a          576 c          454 g          980 t
ORIGIN      
        1 tggagacact aatatgtccc ttacaaagaa caattatacg tttaactatt gctagtccta
       61 atccatttcc ttgttgatta tgcgactgat ccgcttggta aaatttatca aatatatgct
      121 gaacatcata ttgagaaatt cccctacctt tatcagaaat aataactctc agataatgat
      181 tattagtttc ttccattcgt acacttaagt ctacaatatc ctcggaatat tttattgcat
      241 tatctaaaag gtttatccaa acttgcatca gcaagtcaga gtttgagtaa acagtatatg
      301 gttttgaatc cagttgaaaa tttatccttt tagcttgcca acgttctgtt aaactaatca
      361 ttgcttgacg aatttgttca tctacacgaa cagaggatag ctccccaata gtctcctgat
      421 tatccacgcg cgacatttgc aacatcgtat cacataatct tgttaacctt aggctctctg
      481 ttttaacaag acctaataac tcactttgtg tttcttcatc taattctgga tcttgtaata
      541 gttctacaat attagctact gcagcaatcg gagtctttag ctcatgagaa acattagtga
      601 taaagtcttt tcgcatatga tccatatgag ataaagaaac aatcatctga ttaatacttt
      661 cagatagctc atccaattca ttataatact cataggattt acgacgatta ctgtttcgat
      721 aaatatgccc ttcaaagtca ccgcctgcaa cattggacac aatatggcta atatctaaaa
      781 ttggttttgt taaatgataa gaaccgtacc acatactaat actagctatc acaaggctta
      841 aaaaaacaac agcgagagcg ctaacttgta atacatgagt agttaagact ccttgaaaaa
      901 aagtaaaaac tattgctgca actagtaaaa gagaagctag gactgagaaa agcatcgtta
      961 acacaaacat taatgtgaaa taaaatctta acggaaaccg aaatcgcctt agcttagtca
     1021 ttttttgcac gataccctac ccctctcacc gtttcaatct taaaatctgg taagtgttct
     1081 acttttcgtc taattttatt gatgcaagca tctaccaccc gctcatctaa atcagtatcc
     1141 attccccaaa tactatccaa tagttctaaa cgtgtgaaaa tcctattagg atagctaagt
     1201 aagtggaaaa gaaccctaaa ttctttctga ggaaatacaa tttcttgctc aaaatatttc
     1261 aagcttaatt catcttcatt aagacaagta ttcccaataa ttagctgatg ttgagctaca
     1321 atattagctc tcctcaagag tgccttaata cgtaaagcta attcttgtaa ctgcacaggc
     1381 ttaaccatat aatcatctgc accaaggcca aacccttttg tcatatcttc catttgtgat
     1441 tttgctgtca taacaatgat tgggacattg taagcagctg acctgagttc ttgaattaat
     1501 tgataaccat ccatcatggg catcatgata tcagttataa tcaattgtac ttcttctgta
     1561 tctagacaat ttagagcttc ttgtccattt gaagcagtga taaattggta gccctcttgt
     1621 gttaattttg tggtaatgat tttttgttga acaatgttat cctctacaac taatatttta
     1681 atcattaaat cacctctagc ttattgtaac acaagttaga agttttcttt aactaatttc
     1741 ccgtccttta aacgataaac ggtatctact aaatctagaa cgcgctcatc gtgtgtcacc
     1801 ataatagctg cagtattatg tgatttaact tcctgacgaa taagttctgt aacttgacgc
     1861 ccacgttctt catcaaggct agctgttggc tcatctgcca agataacctt aggatgatta
     1921 ataaatgctc tagcaattgc tgcgcgttgc ttttgtcctc ccgataattg gttagggtat
     1981 ttatgtgcac actgctctat tcctaaatct gataataatt tattaatttc ttccaagctt
     2041 gttttcttat catagtgttt tgcaaatcga gcctcttgtt gcaactgttc ttggattgtt
     2101 aaataaggaa gcagttgatg actttgaaat ataaaaccta gtgcttcacg gcgcaattgt
     2161 gttctttgtc tttgagacaa atccgtaacg ttctcatgat tgacatacaa actaccttga
     2221 tctgcaccaa gaagtaaccc tgcaatggat aaaagtgtgc ttttacctga acctgagggt
     2281 cccagtatag ctacaaactc acctgcagaa actgataaat ctaaattatc taatattgat
     2341 aaaagctcat ctccatctgg atagtgtttt gagatgtgtt ttaattctaa gattgctgac
     2401 attattctcc tcccccatta ataacttcga ctgggtcgat tttagcaact tttacaattg
     2461 acaaagcact actaatcatt gcaatgacta aaaatacaaa cgatactaga ataatatttt
     2521 gccaattaat aacaaatggc atttgagctg gtaatacata agagagagca atcgccaatc
     2581 gatctccaac aatgatacca aacagtgcca atatgatgac ttgactcaat tgaaataggg
     2641 caatctctga catttttgtc ccaatagctt tcatgacgct aaattcatga cgtttttgaa
     2701 gtgtgatgat gtagaaaaag acacctaaaa taccagctga agctacgaca aggacccata
     2761 aaatcatatt caaagtggat tgttcagcac tataaccagg aatatgttga atgacatctt
     2821 ttttactaac agcttctaat tgagttggta aatgagatat acttttatca tttttcataa
     2881 ctaatgcctg aggtaagaac tggtattgag gattaatctt tttattaatt tcggtataaa
     2941 tgtctttatc aataaatgcc a
//
LOCUS       DATRNX010000150         2945 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRNX010000150 DATRNX010000000
VERSION     DATRNX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..594)
                     /locus_tag="VBC81_001899"
     CDS             complement(<1..594)
                     /locus_tag="VBC81_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6912903.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDIL"
     gene            687..1036
                     /gene="ssrA"
                     /locus_tag="VBC81_001900"
     tmRNA           687..1036
                     /gene="ssrA"
                     /locus_tag="VBC81_001900"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1305..1943
                     /locus_tag="VBC81_001901"
     CDS             1305..1943
                     /locus_tag="VBC81_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6912904.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(2061..>2945)
                     /locus_tag="VBC81_001902"
     CDS             complement(2061..>2945)
                     /locus_tag="VBC81_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6912905.1"
                     /translation="RHDLCQLMMTSKRFKNLTLSYYQAEISLEFEKQFAAMVFTIPNI
                     DYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTILPYFDKVVLSGHSKGG
                     NLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTKQKCIRLLPEESIVGM
                     MLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLKSICFEKTFQQWMAEL
                     KSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFRELDDDKKRLFNKSLK
                     LLVTIFWGAYHDNSRETK"
BASE COUNT         1016 a          509 c          466 g          954 t
ORIGIN      
        1 caaaatatca ccgccataaa tccacaaata gctataaccg atgatagaaa taggtttcgt
       61 tatcataatg attgtcacaa agcgcttaat agacatatta gatagaccgg taatcatcac
      121 catgatgtcc gctggtgaaa tgggagatgc catacaaaag ataaaaaact tttcatatcc
      181 ggatgtttct agcttagact catatttatc aaatgtcttc tgatccataa atagcaaaac
      241 gaattttcgt ccataaaatt tgactaacca aaaaaggatg acacttccga tgataatccc
      301 aatatagtta taaataaagc ctaaggtagg accaaaaatt aagaaacctg ctactgttgt
      361 aacacctcca ggaattactg gaaaaacaat ctgtacaatt tgagccacaa taaaaatcat
      421 tggaccccaa aattcatact tgtgtactag gtcttttaat tcattgctat cattcaaaat
      481 accaagtttg tacaaataaa caactaagac aagggtcgct attaacgcta agacacccaa
      541 agccttaatg accttctgcc aaaagcggta acgttttgat aatttcatat tcatgcttta
      601 atcataacac aactgttaaa tttttgctat tttaaatagt atttgttata ctatattggt
      661 aagtttttat gctttattct acttttgggg tcgttacgga ttcgacaggc attatgaggt
      721 atattttgcg actcatcggc agatgtaaaa tgccagttaa atataactgc aaaaaataca
      781 aattcttacg cattagctgc ctaaaaaaca gcctgcgtga tcttcacaag attgtttgcg
      841 ttttgctaga aggtcttatt tatcagcaaa ctacgtttgg ctactgtcta gttagttaaa
      901 aagagattta tagactcgct atgtgagggc ttgagttatg tgtcatcacc tagttaaatc
      961 aatacataac ctatagttgt agacaaatat attagcagat gtttggacgt gggttcgact
     1021 cccaccggct ccatatcatt ttatcataac ttactataca tacttaaaac cttgttatta
     1081 caaggttttt attttgtttc tttgctcata cattgcccct atcttaggaa aaaaatactt
     1141 ggaacagact ctaacattga attaggccac ctcattagct cactctttgt caatatccga
     1201 gaataagttc tttatcattc taatttatag aatttattaa aaaagataag gtttttagag
     1261 gaaaaaatgt tataatctat ttagcctacg attggaggct agttatggct agtattttaa
     1321 aaaaacattt agagaaacgt ttagagctta aaaatgatcc tatgaggaca gatatgagta
     1381 ctggtgccag ttaccctaaa tatggattct cggtaggaaa gtacacatat ggatatcaac
     1441 aattctttta cgaaggcgtt aacttaaaag aaattggagc attttgctca atagcccaga
     1501 acgttactat tactggtatg aatcatccaa ccgatcacat taccactaac ccttttattt
     1561 actataagtc aaggggattt ataaatgagg atagggcgga cttaatagat gaaaagaaaa
     1621 atggaaaagt tattattggc aacgatgtct ggataggaac taatgtcact atacttccat
     1681 ctgttacaat aggtaatgga gcaattattg aagcaggttc agttattacc aaagacattc
     1741 ctgactatgc tgttgtcgct ggaacacctg ctaaaattat caaatatcgc ttttcagagg
     1801 aagaaataac attattaaat gcatctcagt ggtggaattg gtcagatgaa gctattaagg
     1861 aacatattag cgaattttca gataagaggg aattcttcaa tactctaaaa agtatttctg
     1921 aaaacaaaaa tcataaactc tagaatgact tcatcaaata gcacactaaa atactactca
     1981 gcataatatg agtagtattt tagtatgcta ttttgctagt ttatttagca catgtttaga
     2041 ctaatgcaat tctaggaaag ctacttagtc tctctgctat tatcatgata agctccccaa
     2101 aatatggtta ctaataattt taaagatttg ttaaataacc tttttttatc atcatctagt
     2161 tctctaaagc tatgaaaagc tttcatcatt tttgctctgg aagccaagtt gaagtcatcc
     2221 aaactagata cgccacttga taggaagcta tcaaataata aatcaaaaaa taattttctt
     2281 tcttgacttt ttaattcagc catccactgt tgaaatgtct tttcaaaaca aatacttttt
     2341 agcgatagtc ctgctccagt ctttaaaata gctggttctt gaatttccca agttgtaaca
     2401 tcgtgttgca gaattccacg cgcattgcta ctaataatta gcggctctat atcactataa
     2461 agcatcatac caacaataga ttcttctggt agtaagcgaa tgcacttttg tttagtagtt
     2521 ttatactctg tagtaggtaa caaagttttt tgtagtcctg gggagtcaat aagccaaatc
     2581 aaatcaattt ttgcttttaa atctggcttt gtaaacattg cagcgtacaa ggctaaatta
     2641 ccacccttag aatgacctga gagaactact ttatcaaaat aaggcagtat tgtatttaag
     2701 tatttaatcg ctgaacgatg agccgatatt tctctcatat acgtcaattt aaaatcttct
     2761 ttccaaccaa taaggttggc atcagtacca cgaaatacaa cctgatgata atcaatatta
     2821 ggaatagtaa aaaccatagc tgcaaattgt ttttcaaatt ctaaactaat ctcagcttga
     2881 taataagata aagttaagtt tttaaaacgt ttcgaggtca tcattaactg acataaatca
     2941 tgtcg
//
LOCUS       DATRNX010000151         2825 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRNX010000151 DATRNX010000000
VERSION     DATRNX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(80..1147)
                     /locus_tag="VBC81_001903"
     CDS             complement(80..1147)
                     /locus_tag="VBC81_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912906.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     DILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(1271..1675)
                     /locus_tag="VBC81_001904"
     CDS             complement(1271..1675)
                     /locus_tag="VBC81_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO6912907.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(1697..2443)
                     /locus_tag="VBC81_001905"
     CDS             complement(1697..2443)
                     /locus_tag="VBC81_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6912908.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(2452..>2825)
                     /locus_tag="VBC81_001906"
     CDS             complement(2452..>2825)
                     /locus_tag="VBC81_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912909.1"
                     /translation="TFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVERVS
                     KIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKERFN
                     SALEGLMKTARQLIQDDSNWK"
BASE COUNT          778 a          521 c          493 g         1033 t
ORIGIN      
        1 tatatttaag gcggtagacg gatttgaaaa ccttggttta tcaaggtttt tggttgtttc
       61 gccccaaatt cgccccaaat tatgcgacta aaaaaatatt tttgattttc tcaaaatttc
      121 tctcagccag tgcttccatc tgatgtgtat aaactttcag agtaatatct ggactttcat
      181 gtccaaggag cttagaaatt gtcacaatgt caaccccgtt caaaatcaaa aacgaagcgt
      241 atgtgtgccg taatgagtga tttctgactt tcctacccac aattttctta atcagtttat
      301 tgcaagctga attagataca ccaaagcaca ctctattctt gatattagct tgccaatgat
      361 ttttcttata ttctcgaaga atatctattg tatttgaatc aattggaact tttctttttg
      421 atgactcatt cttcagatct gcaaaatctt gagtattcga gtaatcaaaa ctcttgttta
      481 tatccaaaat gtcatttttg aagtctatat cgctccaggt tagtcccatg acctctgaga
      541 accgcaaacc agtgacagaa agaaggtaga gagtgaaata ggatacatac tggatattct
      601 ctcttgtgag ggataaaaga gccttatact cactttcttc taagaagtca ttttcctcag
      661 ctcttgattc aatttgtgat ttaacctttg catcatcagc aaagttaaaa tgaataactt
      721 gttccctcac agctactttc atagctccct tgatttggta gtggaacttc tcaagagttt
      781 cttgagcata tttctcccca aattcattta gccgtttttg ataatagagt ggtgttatat
      841 ccttaacctt tatatcttta aaataggttt tgatgtgtct aaagttcttt gtgtaggttt
      901 cccaggtctt atcctttaca taaggacgtt tataaacttc tgaccaagtt ttgacaaaat
      961 catatagagt aacttctcca tcggtcagga tattttgaga tagttttttc tcaatatccc
     1021 tagcagctgc ttgagcaagt tttttggttt tgaaaccact cttagacttt tgacggaact
     1081 taccgctctc atctttatat gaaatgcgat attcccagcc attatcacgt tttcgataat
     1141 aagccatttg tttttcacct cattttttga taaaatgggt atagtaaaga gacctactgc
     1201 taaagcaggt ttctcactat ccacaattgc cttacgcttg caccgaccaa agttgagcgt
     1261 agggcttttt ttatttttta actttatttt tcaacgcatt ctcgatagca tttttaagct
     1321 caagaatagc tattttctca tctttactga aggttactgt attttcgtct tttactgcat
     1381 cgaaaacacc gcccttagta tcagatgaac caggataaac caattgtaga tagccaacag
     1441 ttgaactagg ttccttgagt tgataagctg taatttctga taatagaatt gacttttctc
     1501 catctaagcc atgtagcaac atatttgaca tgttgctttt tcttgcgatt ctgatgaagt
     1561 aatcatcaat acgtacaatt gtttttgatt ttttgaattc aaaaacacgt tcgttaggct
     1621 ctgatgtaaa gagttctact tcaatttctt tatcttttcc gccaaataat gccattattg
     1681 ttgtctcctt ttgtttttat tgtaaccgta aaatgttaaa aatctgccct ccaccttgac
     1741 ggtagtttaa aactaacttg agggcgtagc tttcagcata ctgcaagtta tagatgagat
     1801 agttcatcag tctgttgtgt agtgctggtt tgctaatatc tgtttcacgt tgaatatctt
     1861 caaagctctc tctagttgtg agggcttttt tgagtcgttc atctgttagg tagaggattg
     1921 aagccactgt atcagcttct ttctcaattg gaatcagctc tttcgggtac ttctcactag
     1981 cgtttttact catgagagac atatagattg gctgattttc tgtttgatta aggtgacagt
     2041 gtatgtgact aagttcgtgt aaaatcgtga agataacacg tcctttggtg tttgtatgtt
     2101 gattgatata gatgatgtag cgtccaaggt ctaggtcagg gacagtcaat ccagcacagc
     2161 tttcgcagag taccctatca gtataagtaa cggttctatt tgataccagc cctctgtact
     2221 tgatgtcttt atctgtggga tgataatcag gtaattctgg ataacgttct ttcatctcat
     2281 cgtaatcaag aaagttaaag agaataggat agttttgttc aaaatgttta atgacatctt
     2341 gataactaac gttctctttc attttatcaa ttgagcttat tatttgataa gccttattgt
     2401 gatagtcaaa atactgttcc cttgatagcg gtgtgtattt caataagtca cctacttcca
     2461 gttagaatca tcttgaatca attgtctagc tgtcttcatt agaccttcaa gagcagaatt
     2521 gaaccgctct ttttcagaag ctgaaagtcc ttctgtttcc ttacgaaaaa gaacaatagc
     2581 ttgctgagtt agatcatcaa ctgcagtatt atcttggcca tcggcaatct taggattttc
     2641 tgttctaccg agaaggtagt cagtgctgac attgaaataa tctgctattt ttgagacacg
     2701 ctcaacattt ggtgtagatt ttttcatgtt gtatatggta tttctaggaa aatccaactg
     2761 ttcttcaagt tgattgagtg aaataccttg cttgtcagcc aattccttga tcttttcaaa
     2821 tgtaa
//
LOCUS       DATRNX010000152         2794 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRNX010000152 DATRNX010000000
VERSION     DATRNX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            312..1157
                     /locus_tag="VBC81_001907"
     CDS             312..1157
                     /locus_tag="VBC81_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6912910.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(1200..2447)
                     /locus_tag="VBC81_001908"
     CDS             complement(1200..2447)
                     /locus_tag="VBC81_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912911.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          954 a          440 c          550 g          850 t
ORIGIN      
        1 aaaattgaga gattctcatg atagacacag agagatttta atgtggtttc aatttgtcta
       61 atgtaggaat aatcaccagc caaaacaact gttttttgat aagtcataaa aaatccttta
      121 tttatattag ttagatatag taaatataaa tttaaaaact attagtatta ttctatcata
      181 ggtgagtgtg attcagaaat tattattaaa ttaattatgg agtttatata gtacaacata
      241 ttctttttca gaaaaaacag ctatattttg ctatacttta atcaaatatc aatattagaa
      301 agtgattgcc tatgttgtct gttggtttag ttttagaggg tggcggaatg agaggtcttt
      361 atactgctgg agttttagat gcttttctag atgcaggaat aaaaatagat ggtatcgtat
      421 ctgtctctgc gggtgcattg tttggtgtta attttgtatc tagacaacga gagagggctt
      481 tgcgatacaa taaaaagtat ttatcccacc ctgaatatat gagtctaaga tcatggcttc
      541 gaacagggaa ttttgttaat aaagatttca cctattatga agttcctatg aaattggatg
      601 tatttgacga tgaagcattt aaaaaatcaa gtattgattt ttacgtagtt gctacagaga
      661 tgacatctgg taaacctgaa tattttaaaa ttgatagtgt ttttgaacaa atggaaattt
      721 tacgtgctag ttcagcatta ccagtagtct caaagatggt tgattggcag gggaaaaagt
      781 acttagatgg tggtttatct gatagtattc ccgttgattt tgcccgtggt ttaggatttg
      841 acaagttgat tgttgtgatg actaggccgc tcaattatca gaaaaagcct tcaagtggac
      901 gattgtataa aactctgtat aggaaatatc ctaattttgt aaagacagcc tcgaatcggt
      961 accaacagta taataatagt cttgaaaagg tcatgagcct tgaaaaaaca ggcgatctat
     1021 ttgcaattag accgagtaag agcttggtta ttggccgctt agagaagaat ccggataaac
     1081 ttgatagtat ttatcagctt ggtatgaaat atgctaaaag tgtgatgcct gagctgaata
     1141 gttatctaat gaaataaaga aaacagtatt tctcaattga ggaatactgt tttgtaatat
     1201 cagtcagttg gtttgacatc taattgtgtt aaattagtgt gatatttttg acgttttaat
     1261 tgcttaatgc ttagcattga taagaaacta acgatataga gacttgctaa gaaaagcatg
     1321 accatagaga catttgaaac gtctagaatc attccgatga taacagacga aaagccacct
     1381 attgcacgac caacatttaa gataagattg ttagctgttg aacgaatatg atgtggatag
     1441 agacgtgtaa tcatggcacc atagcctgca aacataccat taacaaagaa gccgacaact
     1501 gccccgccta taatcataga aggcattgtt gttgcaaatt gaaagagata gacacaaatt
     1561 gcagatgata gaaggaaaca cccgtatact aaacgcggtc caaatttatc taacaattga
     1621 ccaaatacta gcatgccaag acacattcca aaaatggttg caatcatcca taatgatgaa
     1681 ttcttgacag aaacgtttaa gttggtttgg ataatagttg gtaaccagtt catcatgcca
     1741 aagtatccgg caatctgaac agttgtcata accattagtg ctaggctctg tgtggctaat
     1801 acaggagtat caaataaagc ttttatactg atatctagag gttcatcatc tgcgtctgta
     1861 ttatattgat ctaagatatc tttatcatca atagcaaatt gcatccatag gacgagaact
     1921 ataggaagaa ggccaaagag aaaaagtccc cgccaaccga gtgctggtgc tagccatcct
     1981 gctaagagtg ctgagctaat agatccgact tgaccagcga taccatttag tgaagaaatc
     2041 cggcccattt tattagctgg gacaattcct gccataatgg caatagcgat accatattcg
     2101 ccaccaacgc caattcctgc aatgaagcgc atgatataga gatatgataa attagtagtg
     2161 aagaaaacta aaccagttgc gattgaaaag attagaatcg tccatttgaa aactttgaat
     2221 ttattgtagc gatcagctaa taaaccaaag attagtcctc ccacgagcat tcccaagttg
     2281 gtaattgtcc caatccaacc agcctgtgcc gcagataaat gaagatcagt aatgatagtt
     2341 gacatagaaa atgcaaggaa catgatattt aaatcatcgg ttccggatgc gacaatggca
     2401 gcgattaggg cgcgccaatt attcttattg atatttaatg gtgacataag tctcctcctt
     2461 taatgatggc tcacagacca ccgttattaa atactgtcaa tagaaaaaat tctaaagcag
     2521 agatgtcata gctgcttcaa ctcattataa tgtaaaagaa aaaactcaag ctgctaccct
     2581 tgagttctat aaacgctcgc aaaaatagta ggatttctgt atagaatcct attttggtcg
     2641 cttatatgtc tcacaggaca cggttaaagc attatgtcat ttggaaatag tatagagtaa
     2701 gtttagttta ttgtcaagga taagtgggga gagaacgcta aattctcaaa gtaagttcca
     2761 cctctagtag aaatggaatt tagtctgata aaaa
//
LOCUS       DATRNX010000153         2794 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRNX010000153 DATRNX010000000
VERSION     DATRNX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(31..387)
                     /locus_tag="VBC81_001909"
     CDS             complement(31..387)
                     /locus_tag="VBC81_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6912912.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            complement(454..2772)
                     /locus_tag="VBC81_001910"
     CDS             complement(454..2772)
                     /locus_tag="VBC81_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6912913.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
BASE COUNT          858 a          540 c          437 g          959 t
ORIGIN      
        1 cgttcaacta aatcgaacgg ctaagtagta ttaaaataag aatgttaaaa gagctaatat
       61 aggtaaacca ccctgtttta ataagatttt tttatcaact gtcaaagcac cataaacagc
      121 aactagcaac acattgatta aaaaaatagc tacaatttct tgattctgtg aaatatataa
      181 cccataaagg aggaatagac caatcaagcc gttgtataca ccttgattct taaaaagttt
      241 aataacgggt aaatatgaca actcttcttt agacatacta aaaatcttcc cagtcatatt
      301 tgactgggtg gctaacgtct ccaaatacat aatataaaaa tgttccaaag cagttaatgt
      361 tgcaataatt attgttaaag tagacatatc ttctcctcct aaatactttg acatagcata
      421 tcatactgtt agtctatcac tagatacttt ctattaatct tcttgtttac gataaataag
      481 aagagcaagt atcatcgatg atactaaaaa gattactagc attctccatt gatggttaac
      541 gtctcctgtc aacgagatgg tctcccttaa tcctgaaaca gagtaagtca tcggtaaaaa
      601 tggttgaatt gtttgaaaga acttaggact caattctatt gggtaagttc ctgcgcttga
      661 accaagctgg aataataata ttaataacga caagaaggaa ccatacctat tatcccatcc
      721 cactaaagca gttactaatg ccatcaaagt ccatgctgtc aataggataa taaagtaggt
      781 ttttccaggg taatctggtt taagacctat gaattgaact gcaaaaaata agatagttgc
      841 tgctagagtt gcaatgaatc cattgattaa taatttattt ttagcccaac taaaacgatt
      901 agctggctct ttgccagata aagctttcgc aaaaataaca ttggctgata aagctaccac
      961 catcaacgca actgaaatca tgtatggtgc cattccaaca ccattcgtat caacgctatc
     1021 ataatcttca tgcttgatag tgactgggct agatacagca ttggcattat ccttgtttac
     1081 agaaaccatg gacaactggt ccccagcttt acccaaagaa cttgataatt gagaaacacc
     1141 acctgacaac tctgtcaacc catctgtcaa ctgatgtcct cccccagcaa gcttattaga
     1201 accatctgca attctatcag aaccactagc taattgcccc gctccatctc tcaattgatc
     1261 cgatttacta tctaaagttt gagctccctg acttgctttt tgtgttcctg aaatcaattg
     1321 attggattta ctatctaaag tttctgcacc tttgcgtaat tgccctacag cattcgtata
     1381 atccgtgatt ccttttgtca aggtttgact accttgatat aactgcttag tagccgttgg
     1441 caatttttga attttatcta aatctgagat agcttttaat actaaattgt tagctgttcc
     1501 tgacaaagtt tgaaaatttt gtgagttatt attagaaggg gctgaactag atgcttcact
     1561 aagcacagtt ttcatatttt ctatatttga agaaatattt gctgcaattg tctcagctga
     1621 agtaggtgtt ccagtaaccg ctgaggtaat ttgagattgt tgttcagggg ttaaactttt
     1681 aaaagctgac gttgattgta ccgcagtcat ttgcttttgt ttatcagcag cagccgtatt
     1741 catcaaagcc gttgtacttg attcaatagt tgataaaaaa ttaaataact tactattagt
     1801 atcgctatca gtagttgctg tcgattgtaa atttgtaata atctgattta gattctttaa
     1861 tccatctgaa aggttctgca aagtacgttc ttgctctggt tttagttgag ttgcttgtga
     1921 caattgttgt aaaccaaccg tcaacttttc tgatccttcc accaatgctt gagacttatc
     1981 atctaatttt tgactacctt cactcaactt atgcacacct gttgtgtaag cagtaacacc
     2041 atctgataat gtttctaacc cgttagatag ggttcccaca ccatctgtat aaaggtttat
     2101 tccactattt agtttattag ctcctccaga aaaagtttga ctagagcttg ctaaaacatc
     2161 taaattactt gtcaaggttt ggctcccatt ctgtagctta ttagaaccat ttaataattc
     2221 ttgactccca tcagcagctt tattgatacc tgttttcata gaacccatat ttttaaaaac
     2281 tgattcagta taagtccctg taatagattc tgctacctca tcttttagtt tattagctgc
     2341 tgtctcactc atttttgaag ctacaaaact acgcccctta gtagttttat aagtaatatt
     2401 taacctttct ggtttttctg tcatgagagt tgtcgctttt ctagaaagat ctttaggaaa
     2461 ggtaataacc atataatagt gacccttttc taattctttt tctgctcttt tagctgttgt
     2521 aaaatggaaa tctaagctat catttttaga caaattgtcc tccatatctt tgccaattga
     2581 aatagttttt ccatttaatt tagccgattt atcttggtta acgactgcca caggtaaatt
     2641 tttagtattc ccataaggat cccacattga gcttagaaag ataacattat atagcgttgg
     2701 aatcagtgca accccagcca tcgtaatcca taattttggg ctttttatga ttgcttttaa
     2761 ttcgtctaac attttttctc ctattttgga cata
//
LOCUS       DATRNX010000154         2749 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRNX010000154 DATRNX010000000
VERSION     DATRNX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBC81_001911"
     CDS             complement(77..1459)
                     /locus_tag="VBC81_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912914.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBC81_001912"
     CDS             complement(1467..2681)
                     /locus_tag="VBC81_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6912915.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          936 a          580 c          423 g          810 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tattattcta acaatctgct cttatatag
//
LOCUS       DATRNX010000155         2714 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRNX010000155 DATRNX010000000
VERSION     DATRNX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            91..963
                     /locus_tag="VBC81_001913"
     CDS             91..963
                     /locus_tag="VBC81_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6912916.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            1029..2648
                     /locus_tag="VBC81_001914"
     CDS             1029..2648
                     /locus_tag="VBC81_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912917.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
BASE COUNT          864 a          448 c          502 g          900 t
ORIGIN      
        1 ataaaaatgt tatgactgag ttgtcaatat tatttattga cagctttctc tttttatcat
       61 gttattatta ctaaagttaa ggagcttatc atgtctaaaa aaattaagaa ttttatacta
      121 ttaaatattg gatctctaat tgttgcattt ggtttcaacg ctttctttct tcataatcat
      181 attgtcacgg gggggattag tggtttggca gttagttttc atgaagtatt tggttgggat
      241 tctgcttttt ttattatgat tatcaatatt ccattgctcc ttctttgcta ctttggctta
      301 ggtaaacaaa cctttttaaa aactgtctat ggttcttgga tttttcctgt ttttattaag
      361 ttaacacaaa gtgtaccaac tttgacccac aacccactcc tcgcagcact ttttggaggt
      421 gttattgtag gatgtggttt ggggattgtt ttttggagcg actcttcaac tggtggaacg
      481 gggattatca ttcaattctt aggaaaatat actcctataa gccttggaca aggggttata
      541 ttgattgatg gacttgttac aattgttggt ttcctagctt ttgacagtga tacggttatg
      601 ttttctatta ttgggttgat aactattagt tatattatta atgctatcca aactggattt
      661 acaaccttaa gcactgtctt aatcgtttct caagagcacc aaaaaattaa gacatatatc
      721 aatactgtcg cagatagagg agtaacagaa attcccgtta aagggggata ttctggaact
      781 aatcaaatca tgcttatgac aactattgct ggttatgagt ttgctaaatt acaagaggca
      841 atagcagaaa ttgacgaaac agccttcata acagtaactc caacatcaca agcttctgga
      901 cgtggattta gtcttcaaaa aaatcatgga cgtcttgatg aagacattct tatgccaatg
      961 taatcgattg ataattaaag ctttttctag tagaatagca gatagaatat acacaagagg
     1021 taacaaaatt gcttacagtt tcagatgttt cactacgttt tagtgatcgc aaattatttg
     1081 atgaagttaa cattaacttt acagcaggaa atacctatgg tcttatcgga gcaaatggcg
     1141 ctggtaaatc aactttcctt aaaattttag ctggtgatat tgaaccaaca acgggacata
     1201 ttgcacttgg cccagatgaa cgcctttctg tacttcgcca aaatcatttt gattatgaag
     1261 atgagcgcgt cattgatgtc gttatcatgg gtaatgaaac actttatagt atcatgaaag
     1321 aaaaggatgc tatctacatg aaagaagatt tttctgatga agatggtgtt cgcgctgcgg
     1381 aattagaagg agaatttgct gaactcggtg gatgggaggc tgaaagtgag gcttctcaat
     1441 tactccaaaa tctaaatatt tcagaagagc ttcattatca aaatatgagt gaattagcta
     1501 atggagacaa agttaaagtt ctccttgcaa aagctctctt tggtaaacca gatgtacttc
     1561 tccttgacga gcctaccaat ggacttgata tccagtctat tacttggtta gaagatttct
     1621 tgattgactt tgaaaataca gttattgtag tttcccacga ccgtcatttc ttgaataaag
     1681 tatgtacaca tatggctgac cttgattttg gaaaaattaa actgttcgtc ggcaactatg
     1741 atttctggaa agaatcatca gaattagcag ctcgtttaca agctgatcgt aatgctaaag
     1801 cagaagaaaa aattaaacaa cttcaagaat tcgttgcacg attttcagcg aatgcttcaa
     1861 aatcaaaaca agcaacttct cgtaaaaaaa tgcttgataa aattgaactt gaagagattg
     1921 taccatcaag ccgtaaatac ccattcgtta actttaaagc cgaacgcgaa atgggaaatg
     1981 atttattgac cgttgaaaat ctctctgtca ctattgatgg ggaaaaaatc cttgataata
     2041 tcagtttcat tcttcgtcct ggtgataaaa ctgcacttat tggacaaaat gatatccaaa
     2101 ctactgcact tattcgagca ttgatggggg atatcgaata tgaaggaaca atcaaatggg
     2161 gagtaacaac tagtcgttct taccttccaa aagacaattc acgagacttt gcttctggcg
     2221 agtcaatctt agaatggctt cgtcaatttg ctagtaaaga agaagacgat aataccttct
     2281 tacgtggctt cctaggtcgt atgcttttct ctggagatga ggttaacaaa tctgttaatg
     2341 tcttatctgg gggtgaaaaa gtacgcgtta tgctttcgaa attaatgcta ttaaaatcta
     2401 atgttcttgt tcttgatgat ccaacaaacc acttagattt ggaatctatt tcaagtctca
     2461 atgatggact aaaagatttc aaagaatcta ttatttttgc aagtcatgac cacgagttta
     2521 ttcaaacttt agcgaaccat attattattc tttctaaaaa cggagttatc gatcgtatcg
     2581 atgaaactta cgatgaattt cttgaaaata ccgaagtaca agctaaggtc gctcaactct
     2641 ggaaataatc ctttggcata tctctaacct aataaacgtg tcaagattga aaatcttaaa
     2701 actatattaa tcat
//
LOCUS       DATRNX010000156         2637 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRNX010000156 DATRNX010000000
VERSION     DATRNX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            156..686
                     /locus_tag="VBC81_001915"
     CDS             156..686
                     /locus_tag="VBC81_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912918.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQINGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            778..1001
                     /locus_tag="VBC81_001916"
                     /pseudo
     CDS             778..1001
                     /locus_tag="VBC81_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1333..1809
                     /locus_tag="VBC81_001917"
     CDS             1333..1809
                     /locus_tag="VBC81_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912919.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            2065..2402
                     /locus_tag="VBC81_001918"
                     /pseudo
     CDS             2065..2402
                     /locus_tag="VBC81_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          983 a          348 c          494 g          812 t
ORIGIN      
        1 aagcaattaa atataaaaaa tcctaaaaaa tcatctattt cggttacaaa aacggaaagt
       61 aaagaatatt attatacaat cagtattgat actgattctg agatatattc tatattcgaa
      121 ggtattacat tagaggatgt aaaggagttt tctaaatgag tccaagtgag aaattagata
      181 gaagaattga ttcttcatca gacctctccc agtatgtata tgattttgaa aagcaatata
      241 cagaatatat gaagaagggg tattctgaaa aaaatgcgag tatacaagca cgacgaaccc
      301 tgaatgaaat actacaagaa aataatccag gattgagatc tagcgacgcc atgtatgata
      361 aagaaacagg tattgcagct attgctgtaa aagataaaaa tactggtgag acttatatag
      421 cttatgcggg tactaataag gatgctgatg gggcaaccga ctttatttca gatgcaaata
      481 tcggtgctaa taactctgct tatttaaaac gattaggaga caaagctact agcttttatg
      541 atcgtgttca aattaatggc gataatatta ctgtcacaac aggtcattct tatggtgact
      601 ttctcgcttc tagagtggca attgagctaa ttccattctt tattaacttt agaaaagtta
      661 atgattcatt tcacttttgt tattagcttg tgatcactta taaagtgaca gctagttcat
      721 gaaatttctt aggagttttc tggctttaaa ggtaacctat gatttaaaaa acatctcatg
      781 gaattcaatg cggaatcctc aacagtcttt tgtggactaa cagaagttca ggatgtcgtt
      841 tcggcaggcc ttaactattc tcagaaggtt ttggaaattt caatggtaca tttgatacct
      901 caggtaaggg attagaatgg acaaaaacaa ttcaaacagg tatgaaaaaa cgagaagaga
      961 aaatcaaata ccctcatttt acaattgttg acataaagta attaaacaaa atccggaaaa
     1021 tttatattaa gtaatttttc taaaaagtca tataggcata cttttaaagt tcctctaaac
     1081 ttaataagga agaaacgttt tgaaaaaaga taaatatatt gcattgaatt ggttgtgtgc
     1141 gatagccacg ctatactatt aagttattac aaactatgaa ttacacctca aatgttggag
     1201 ataaaaccta tcgtttgggg tgttttttgg taaaatagaa tgaaaagagt tactctgtgt
     1261 cattactaag ttaaaaaatt atactaactt aattattaac gttttattga ctgagttact
     1321 cttttttaaa aaatgtatat cattccagct ggttactcac ctgaacgttt agactcagaa
     1381 gtccttacag caaagattga agatgatgaa gccttattgc ggaaaattca agaccttaaa
     1441 gatcatttag ataaaaatac caaaatcttg gcttacgctt ttgatggctt ggaagtcaga
     1501 gtgaaagcta gactaagaaa aaatcgtaag aaactcaaaa accttataga attcaatgcc
     1561 aaatctacct cagtctttaa tgaattgaag gattattcaa atgctgtatc gtctggttta
     1621 acctttgtat ctaaaggctt tagtagtttc aatggccagt tctcttcagc ctcaaatctt
     1681 gattggcaaa agactattaa gacaggaatg agaaaacgag aagaagatgc taaacagttg
     1741 agaaaaaaag ctttagaaga ttcaaataag ctcagaaaaa ttgcatcaaa aaggggttac
     1801 tttgaataat gcagttgaat ttgctaaaga acagaacaaa cccctaaaaa taaaaagata
     1861 accatagata aatatggtcg tgtaaaaatg gataaaactt ttctttatcg aaaagctaaa
     1921 gatggaaaat ctgcatatat ttattctgaa gggagtgatc aatataaaga actaactggg
     1981 aaagaatttg atcctaaaac tacaaatatt atgaaaggta gaaaattagg gacagatggt
     2041 aagatatcaa ttgattttgg tgagattagt aactcaggaa aaaatgcagc tattaagtcc
     2101 cttaaaaatg aaagtccttg aattaaatcc caaaaatgct tttaaaggat ggaatgagtt
     2161 aagcaaaata gctaaaactg ggaaagtact aggatggaca ggtaaaattg ctaatattta
     2221 catgaatgga aaaaaagatc tttttgatga taaaacatct tcaatagatc aaaaaatcaa
     2281 aaactttgct ataggttcag gtgtagatgc attgtccggt gcagcaatag ggactttggt
     2341 aggaggacct gtgggaaccg ttgttggagc aggtgcaggg ctggcatttg attttgcttt
     2401 gaattttaaa gttaaggagt tgggtaatga atcaattaca ggttttgcta aaaaaggttt
     2461 aaaaaatctt gcaggaggat taggattttg aaaaaaagta taaatgtaag taggtatcat
     2521 ggacctttaa ttgaatttga ggttgatgca agttatttaa gaattttttc atatatcggt
     2581 ttacctttat cattgttttg gtattttgtt actcataaca gtgatgactt tggaata
//
LOCUS       DATRNX010000157         2562 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRNX010000157 DATRNX010000000
VERSION     DATRNX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            55..1050
                     /locus_tag="VBC81_001919"
     CDS             55..1050
                     /locus_tag="VBC81_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6912920.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            1058..>2562
                     /locus_tag="VBC81_001920"
     CDS             1058..>2562
                     /locus_tag="VBC81_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6912921.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIK"
BASE COUNT          886 a          357 c          469 g          850 t
ORIGIN      
        1 gattcctatt aatggtagaa aagaagcctg ttgtttttta gataggggaa gtgtgtgagg
       61 acatatatta caaacttgaa tggacattca atcactagta cagcacaaat agctcaaaac
      121 atggtaacag atatagcagt aagcttaggt tttcgtgagc tgggaataca ttcttatccg
      181 attgatactg attctcctga ggaaatgagt aagcgtttag atggaatctg ttccggactt
      241 agaaaaaatg atattgtcat atttcagaca cctacatgga acactacaac ttttgatgaa
      301 aaattatttc acaaattaaa aatatttggt gtaaagattg ttatttttat acatgatgtt
      361 gtaccgctaa tgtttgatgg aaatttttat ttgatggata gaactatagc ttattataat
      421 gaagcagatg ttttaatagc ccctagtcaa gcaatggtcg ataagcttca aagttatggc
      481 ttgacagtaa aaaaaatctt agttcaagga atgtgggatc atcccaccaa tattacttta
      541 caagcagtta atcataagaa gcttgttcat tttcctggga acccagagcg gtttaacttc
      601 ataaagaatt ggagaattcc taccgagtta catgtttata ctgaccataa catgcaatta
      661 cccaccactg tggttaaaga gccatatcaa tcggacgaac aattaattat gaaaatgagc
      721 gaagggggat atggcttagt ctggatggat gaccgtgata aacaatatca atctctttat
      781 tgcccttaca aactgggcgc ttatatagca gcaggaattc cagtgattat tcaaaaaggt
      841 attgctaatc aagatataat agaaaaaaat aatttgggat ttattattga aaaaattgat
      901 gatattagta atatagttga aagtactact gaagaggaat atatggaaat tgttagcgat
      961 gtcaggcggt ttaatccttt agttagacaa ggatatttta cgcgaaaact attaaccgat
     1021 gcagtattta gtgcattaaa tagcatgtga ggggaatatg aaagaactag aagaaatcgt
     1081 agttttccct ataaacaaaa gtttggatta cattttggaa aatcattcgt cagttattcg
     1141 ctttggggac ggtgaatttg acattataat gggaaaatcg attccttatc aagattacaa
     1201 cccagagtta gcagataggt tattgcaact tcttcataag caaagtacga atgatctatt
     1261 agtatgtctt ccagatatgt ttcaaaatcg acaaagatat aatcaatcgg cacaactttt
     1321 ttggaaaaat cattttcaaa aatttggcaa tttctatagg caacattgtt atcaagattg
     1381 gtatggttct tcttttattt caaggccata tatggactta caagataagg agtctgctgt
     1441 agaatcattt aaaaaattaa agttactttg ggacaaacgc gatattttgg tagttgaagg
     1501 ggaaacttcg cggtcgggtg taggtaatga tctttttaat aattgcaata gtttaaaaag
     1561 gattatttgt ccttctaaaa atgcttatat aaaatatgat gaaattcttg aggctgttga
     1621 aagatatgct caagggagga taatcttatt gatgctaggt ccgactgcaa aaattttagt
     1681 aagagattta tctgatagag gatttcaagc cattgatatc ggtcatattg tttctgaata
     1741 tgaatggtat aagatgggag ctactcaaaa aataaaattt tctcataaac acaccgctga
     1801 gtttaacgat gatgaaaata ttaatttaat tgacagtcag aattatattg atgagattgt
     1861 taaaacaata actatcgatg atactgaatt cagggatact gaattaatat caatcatact
     1921 tcctgtctat aatatgtctg gttctttatc aaggtgttta gattctatcc ttaggcaagt
     1981 ttactcaact tttgaggtga tcttagtcaa ccatggttct acagatggaa gtggtaatat
     2041 ttgcgagaaa tatgcgatga aggatgagcg tattcgttta tttcatgaag aacatcaagt
     2101 tattacatct gtatggaact tcgcattaga gaaatctaaa gggaagtatg ttacttttgt
     2161 gaatccagat gattttcttg atgagtctta cttaaattat ctttacaata aattaagagc
     2221 taataaatct gatatctcag caaccaccta tacttttttt aatgaagagg aaaaatgctt
     2281 tgtttatttt gcaacagatg ataattattt tgagaaaaca ttctttgctc aagaatggtt
     2341 agagcaagaa catagttacc acgataattt aaaacaaata tttcctctcc tttctatgaa
     2401 gctctttaga aaatcacttt ttaacaatat cacttttcct ttagaagatt taacaggaga
     2461 atacacactg tataatgttt atttgctatc aaggcacatt acattttctc atattggttt
     2521 atacataacc tctcaagttt tctctcatac tgaaataaaa ag
//
LOCUS       DATRNX010000158         2530 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRNX010000158 DATRNX010000000
VERSION     DATRNX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1967)
                     /gene="secA2"
                     /locus_tag="VBC81_001921"
     CDS             complement(<1..1967)
                     /gene="secA2"
                     /locus_tag="VBC81_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6912922.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKD"
     gene            complement(1964..2509)
                     /gene="asp3"
                     /locus_tag="VBC81_001922"
     CDS             complement(1964..2509)
                     /gene="asp3"
                     /locus_tag="VBC81_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6912923.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
BASE COUNT          789 a          500 c          343 g          898 t
ORIGIN      
        1 aaatccttat cattagcaat atcgttaaaa ttatcctgaa taatttttat cacaccatca
       61 accacaatgg aactatcagc tattaattca tttcgtaatt gataaatatt ttcacgctgt
      121 atacgcaagc tctctccgaa cttaatagtt tgctccctag aagatacacc tttatcttca
      181 cttctgagtt gagcattttt aactatttga taaaaaaagc gtttctttct aggtaccata
      241 tagtaatttg aagtttcttt acgagaatat ttttttaaat actttatcac ccattccgga
      301 gaatgagaaa caattaattc atcctctagc gacacataaa attggctaat ccctgggtct
      361 ccttgacgcc ccgaacggcc cctaagctgc cagtccatac gtaaattagg catcctttcc
      421 gttcctataa ctgcaagtcc tcctaattct ctaacccctt cacctagttt aatatctgtt
      481 ccacgaccag caagaactgt tgcacatgtt actgaacctt tttgtcccgc ttcttttata
      541 atttgtgcct ctttaacaac attatcagct gttaaggtat tatgggcaat gccttctcgc
      601 agcaataact ttgaataaat gttagcaatt tcaactgttc cagaaattaa caatactggc
      661 tgacctttac tatgtctttg tttaacatca tctatagtag cttgcaactt ttctggtaag
      721 ctaacatata ttttatcggc aagatctact cgtttgatcg gaaaatttgt tggtataaca
      781 ataacaggca ggtggtaagt ttttataagt tcatcttcag aagattttcc agttcctgtc
      841 atcccagaca accttgggaa catattaaag agactttgat acgttattga agcaattgcc
      901 ctagattcat ttgtcaaagc aacatcttct tttgcttcaa tagcttggtg aatccctgat
      961 tgaagttttg tgccttccat aacacgacct gttgcgttat ctaacaattt tacttcatta
     1021 tttttaacta cataatcata atctctttta tacaaacaat gagcacgcaa tgctaggttt
     1081 atatgtctat ttaattccca atactcatca ttatataatt ctgaaatatt gaagtactgt
     1141 tttgcgtaac ttacaccctt ctcagtaaga tagacaattt tatccttagc tttatggata
     1201 tattcatcat tttccttaag agtgagtaca aaattattag tgataccata gagattagat
     1261 tgtacacgag gatctcccgc tataattaag ggtgtttgag caatatctaa caaaacagag
     1321 tctgcttcat caacgataac aaaatgaaaa ggtcttagaa attgttctag tttattacta
     1381 gccagattgt gaacgagata gtcgaaacct aaggcacctg cagtagtata agtaatatca
     1441 gattcatata cttctctctt aatagtcgca tctgcttctt cttttttatc aaaaacatta
     1501 ataccaactg tcatacctag aaattgatat acttttccca tctctaatgc atctcgtttt
     1561 gccaaataat cattggtcgt cactaagatt gctcccttgc tagttaaagc attcagatac
     1621 aagggcatag ttgcagttaa tgtttttcct tctcccgttt tcatttcagc tatcgttcct
     1681 tgatgaagag caatagcacc cataacctga gcatcatatg gaaacaaacc taaaattctt
     1741 ttattagcct ctcttatcac agcaaaagca tcaacaagaa tatcatctag actttctcca
     1801 cttgaaagtc tattttttaa tattaaagtt tgatggctta actccttatc tgataattga
     1861 gcgtagtaca cttttttatt atttatctcg tttacaattt tttgaattct ttgcaatctc
     1921 aatttattta gaatagagat tttgggttta acaacaactt ttttcatttt ttatccttag
     1981 aaaatgctat caacgttcta ataagttcaa gctctgatgg taagctgctt tcaacataac
     2041 tgtttagttc aataccatct ttttcttctt gagtctcaat tacttcaaat aattctaggt
     2101 aacaaaattc tacacttata caacccgcac caataagaga aatatcataa aaatatgtgt
     2161 catcaggaac tataaaatct gaggagtgat ctcgaataat agaaaaacct attttttctt
     2221 tttgacgatt ataaaaattt atctgaataa tcacgcatcc ctcaggcaca gtagttaatt
     2281 ttgataccaa ctcataggta tgattaggtt ttagtaaggt aagctctggc aacgttctat
     2341 tagcttgaaa attcgtttta gaaagccatt ttttgattgt ttttcctgta gagaatctta
     2401 gattctcaaa aaaaacacga ttatgctcca aatactttat agtcgaacca taaagataaa
     2461 cattgctact atcagtccaa agcaccttct gtattctttt tttaatcact aaacactctc
     2521 ccaaaatcat
//
LOCUS       DATRNX010000159         2444 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRNX010000159 DATRNX010000000
VERSION     DATRNX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(55..705)
                     /locus_tag="VBC81_001923"
     CDS             complement(55..705)
                     /locus_tag="VBC81_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6912924.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITINHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            799..2151
                     /locus_tag="VBC81_001924"
     CDS             799..2151
                     /locus_tag="VBC81_001924"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6912925.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
     gene            complement(2367..2440)
                     /locus_tag="VBC81_001925"
     tRNA            complement(2367..2440)
                     /locus_tag="VBC81_001925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2404..2406),aa:Asn,seq:gtt)
BASE COUNT          857 a          443 c          381 g          763 t
ORIGIN      
        1 ctagctttca tggcaagata aaaaataaaa aaaacggaat taaacctccc gtttttaata
       61 gttatctaat ttcacaaaaa attgactcag atcgctcact aatacattag cctgcgactg
      121 attcattcca aagtatttgt cttcaatagc ccacacatca ataccagctg caatgccagc
      181 tgcaatcccc ttctcactgt cttctacaat aagaatttgc tgtttaggaa tatctaaaag
      241 ttctgctgct ctattataaa tagctggatt aggtttactt tctgaaaatt cctcaccaga
      301 cagaattacc ttaaaatatg tatccagatt aaaactttct aatgctaaca tgatatcatg
      361 tctagtagaa cttgaagcta gacccaaaag atagcctttg tgatgaagct tttcaatgac
      421 tcttttacaa tcctgaaaaa ttaaatcttt gtatggcaag ggattatcct ctttatagcg
      481 actataatct tgttggagct tatcaatatc ccaggtatca tattgatcac ctaaaacaga
      541 tttccaaacc tgcttcatat ttcctccaat aaaaaaattc attggtaaat ggttaattgt
      601 aattccatgt tgttttaaaa aacgctctcg ccttttataa taaaataatt cagtgtcaaa
      661 tagaacacca tccatatcaa atattattgc tttatattta cacatgtaac tattatatca
      721 ttcttttaaa tttagaggta atgacaaatt ttctgacttt tgttataatt tatctaaaat
      781 tcattcttga ggtaacctat gaaagtagtc aaatttggag gaagctcttt agcatcttcc
      841 caacaattat acaaagtact aaatattatc aaatcagact acactagacg gtttattgtc
      901 gtttcagctc cagggaaacg ctacgaagaa gacttaaaaa tgacagatgc tttgatacaa
      961 tattatcaaa attatattaa cggtaaagat attgttaaag accaaacttg gattatcaat
     1021 cgttatcaag aaattattag tgatttgtct ttaggaagca ctattgcaga agaaattact
     1081 cgctctatag agcagctagc ttcacttccc atagaaaata atcagttttt atatgattgt
     1141 tttttagcag ccggtgaaga taacaacgca aagttagttg caacgttttt taatcaaaat
     1201 gacattcctg caagatatgt tcatccaaac gaagcaggaa ttattgtaac taaagaacca
     1261 tgtaatgcac gaattattcc aggaagttat gataagattg agaacttatg tctatacaat
     1321 gaggttcttg ttatccctgg attttttgga gtcacagaag ataaccaaat ttgtaccttt
     1381 tcaagagggg gatctgacat tactggatcc ctaattgcag caggcataaa agcagacctt
     1441 tatgagaact tcacagatgt tgatggtata tttgcagcgc atccaggtgt agttaagaac
     1501 cctcacgcta tccctgagct tacttataaa gaaatgcgtg aattagccta tgcgggtttt
     1561 tcggttttac atgatgaagc tttacttcct gcctatcgtg gcagaatccc tcttgttatt
     1621 aaaaatacaa ataatcccca acagcctggt acaaaaatag ttttaaagca tactcgtagt
     1681 aacatagcag taactgggat cgcttctgat agccgttttg ctagcataaa cgtatctaaa
     1741 tacttaatga atagagaagt aggtttcggc cgaaaagtac tacaaatttt agaggattta
     1801 aatattagtt ttgaacatat gccaactggc atagatgatc tatccattgt cttacgtgaa
     1861 aaagaattga caccaatcaa agaacaagaa atcttaaatt acctaactcg taaactagaa
     1921 gtagattacg ttgacatcca acacaattta tctacaatcg taattgtagg tgaaaatatg
     1981 aaaagtcaga ttggagtcac tgcaacagcg acacaagcct tatcaagaga aaaaatcaat
     2041 atcaccatga tatcacaagg ttcaagcgaa gtctccatta tgttcgttat agacagtaag
     2101 gatgaaaaaa gagctattaa agcactatat gaaacattct tccaaaaata gtacctatta
     2161 cactacttac actattagat agataacaaa tcgtcctaag taagtttact taggacgatt
     2221 ttatttagaa cataggatag ttttttcaat tttaatcgta actacttggt atcactgaca
     2281 aattcggaca attaagatgc tagccgacct taaggaataa taattccaat aaaaaaaggc
     2341 taaccaaagt tagtctcctt tatctactcc gccagtagga ctcgaaccta cgacatcatg
     2401 attaacagtc atgcgctact accaactgag ctatggcgga ttat
//
LOCUS       DATRNX010000160         2208 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRNX010000160 DATRNX010000000
VERSION     DATRNX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..448
                     /locus_tag="VBC81_001926"
     CDS             <1..448
                     /locus_tag="VBC81_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912926.1"
                     /translation="LLSIHHNGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHV
                     LDHFDYGFRKLNLDVEDLKPFETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYA
                     LEILKQLGCKQYSIGSDGHIPEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            617..>2208
                     /locus_tag="VBC81_001927"
     CDS             617..>2208
                     /locus_tag="VBC81_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6912927.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVW"
BASE COUNT          781 a          354 c          412 g          661 t
ORIGIN      
        1 actattgtca atccatcata atggtaggta tgattatctg caagaagaag ctctgaaagt
       61 accaacaaag gagcttttag cagattactt taatcgtatg gaatttgcca taggccgtgt
      121 ggaagcgcac gttttagatc actttgatta tggttttcgt aagttaaact tagatgtaga
      181 agatttaaaa ccgtttgaaa cgcaattgaa gcgcattttc agaaagatgt tatctaaggg
      241 gttagctttt gaactaaata ccaaatccct ttatctatat gggaatgaaa aactttatcg
      301 ctatgcttta gagatactaa aacagcttgg ttgtaaacaa tactctatag gctctgacgg
      361 tcatattcct gaacattttt gttatgaatt tgatagactt caatgtctgc taaaggacta
      421 tcaaattgat gaaaatcatt tgatatgagg aaatttttga taaaaaagct aggcaatatt
      481 gcttagcttt tttgtaatgt cattgatagt tttagtgaaa atttcaaaaa aataaagaaa
      541 tcacttactt gttgcaagcg cttgcgtaaa ttgttatgat tttattggta acaattcatt
      601 aaaaaaggag aatgatatga aaagaaaaga cttatttggt gataaacaaa ctcaatacac
      661 gattagaaag ttaagtgttg gagtagcttc agttgcaaca ggggtatgta tttttcttca
      721 tagtccacag gtatttgctg aagaagtaag tgtttctcct gcaactacag cgattgcaaa
      781 gtcgaatatt aatcaggttg acaaccgaca atctactaat ttaaaagatg acataaactc
      841 aaactctgag acggttgtga caccctcaga tatgccggat accaagcaat tagtatcaga
      901 tgaaactgac actcaaaaag gagtgacaga gccggataag gcgacaagcc tgcttgaaga
      961 aaataaaggt cctgtttcag ataaaaatac cttagattta aaagtggcac catctacatt
     1021 gcaaaatact cccgacaaaa cttctcaagc tataggtgct ccaagtccga ccttgaaagt
     1081 tgctaatcaa gctccacaga ttgaaaatgg ttactttagg ttacatctta aagaattgcc
     1141 tcaaggtcat cctgtagaaa gcactgggct ttggatatgg ggagatgttg atcaaccgtc
     1201 tagtaattgg ccaaatggtg ctatccctat gactaatgct aagaaagatg attacggtta
     1261 ttatgttgat tttaaattat ctgaaaaaca acgaaaacaa atatcttttt taattaataa
     1321 caaagcagga acaaatttaa gcggcgatca tcatattcca ttattacgac ctgagatgaa
     1381 ccaagtttgg attgatgaaa agtacggtat acatacttat cagcccctca aagaagggta
     1441 tgtccgtatt aactatttga gttcatctgg taactatgac cacttatcag catggctctt
     1501 taaagatgtt gcaaccccct caacaacttg gccagatggt agtaattttg tgaatcaagg
     1561 actatatgga aggtatattg atgtaccact gaaaactaat gccaaagaga ttggttttct
     1621 aatcttagat gaaagtaaga caggagatgc agtgaaagtt caacccaacg actatgtttt
     1681 tagagattta gctaaccata accaaatttt tgtaaaagat aaggatccaa aggtttataa
     1741 taatccttat tacattgatc aagtgcagct aaaggatgct caacaaactg atttaacaag
     1801 tattcaagca agttttacaa ctctagatgg ggtagataaa actgaaattt taaaagaatt
     1861 gaaagtgaca gataaaaatc aaaatgctat acaaatttct gatatcactc tcgatactag
     1921 taaatctctt ttaataatca aaggcgactt taatcctaaa caaggtcatt tcaatatatc
     1981 ttataatggt aacaatgtca cgacaaggca atcttgggaa tttaaagacc aactttatgc
     2041 ttatagtgga aatttaggtg cagttctcaa tcaagatggt tcaaaagttg aagccagcct
     2101 ctggtcaccg agtgctgata gtgtcactat gattatttat gacaaagata atcaaaacag
     2161 ggttgtagcg actacccccc ttgtgaaaaa taataaaggt gtttggca
//
LOCUS       DATRNX010000161         2157 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRNX010000161 DATRNX010000000
VERSION     DATRNX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..520
                     /locus_tag="VBC81_001928"
     CDS             <1..520
                     /locus_tag="VBC81_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912928.1"
                     /translation="YFLYFKDSNFRDKVFDVITKSFNNKNQNIKIDIKNQNIKGDNYE
                     SGSSRNDVSGSGNIVGTNIDNGSQVSLSGDISNGSTIIKDSNINNADIHKSVEIPWTD
                     MVEQLNLIINTQLSTADKAIYSELLEISEAEDERKLKNFVNKMSSFFKNDYVQNTITG
                     VLGNVISSILMK"
     gene            538..1836
                     /gene="purB"
                     /locus_tag="VBC81_001929"
     CDS             538..1836
                     /gene="purB"
                     /locus_tag="VBC81_001929"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6912929.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            1989..>2157
                     /locus_tag="VBC81_001930"
     CDS             1989..>2157
                     /locus_tag="VBC81_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912930.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQ"
BASE COUNT          716 a          352 c          467 g          622 t
ORIGIN      
        1 ctattttctt tattttaaag attcgaattt tagagataaa gttttcgatg taattacaaa
       61 atcgttcaat aacaaaaatc aaaatataaa aattgatata aaaaaccaaa atataaaggg
      121 cgataattat gaatctggat ctagtcgaaa tgatgtctca ggtagtggta atatagttgg
      181 aacaaatatt gataacggta gtcaagttag tttatctggg gatatatcaa atggttctac
      241 tatcataaaa gattctaata ttaataatgc cgatatacat aaaagtgtgg aaattccatg
      301 gactgatatg gttgagcagt taaatttaat tattaacact cagctatcta ctgcggataa
      361 agcgatatat tcagaattgt tggaaattag tgaggcagag gatgaaagaa aattgaaaaa
      421 ttttgtcaat aaaatgtcta gtttcttcaa aaatgattat gttcagaata ccataactgg
      481 ggtgttaggc aatgtcattt ctagtatttt aatgaaataa gttaaaggag acaaacaatg
      541 atttcacgtt attcacgccc tgagatggcg gcaatttgga cagaggaaaa taaataccgt
      601 gcttggttgg aggtcgagat tttggctgac gaggcatggg ctgagttggg tgagattcct
      661 aaagaggatg tggctaagat tcgcgagaag gcggattttg acattgaccg cattcttgag
      721 attgagcagg acacgcgtca cgatgtggtg gctttcactc gtgcggtttc tgagacgctt
      781 ggtgaggagc gcaagtgggt gcactacggt ttgacgtcga ctgacgtggt ggacactgcc
      841 tacggttacc tctacaagca ggctaacgac attatccgtc gtgatcttga gaatttcaca
      901 aacatcgtag ctgacaaggc taaggagcac aagttcacca tcatgatggg tcgtacccac
      961 ggtgttcacg ccgagccaac gactttcggt ctcaagttgg cgacttggta cagcgagatg
     1021 aaacgtaata ttgagcgttt tgaacatgct gccgcaggtg tggaagctgg taagatttca
     1081 ggtgccgttg gtaactttgc caacattcct ccatttgtgg aaaaatatgt ctgtgacaaa
     1141 ttgggcatcc gtccgcaaga aatttcaaca caggttcttc cacgtgacct ccacgcagaa
     1201 tattttgcag tacttgcaag catcgcaaca tctatcgaac gtatggcgac agagattcgt
     1261 ggtctgcaaa aatcagaaca acgtgaagtt gaagaattct ttgccaaagg tcagaaaggt
     1321 agctctgcta tgcctcacaa acgcaaccca atcggttcag agaacatgac agggctagcg
     1381 cgcgtgattc gtggtcacat ggtgacagct tatgagaacg tgtcactttg gcatgagcgt
     1441 gacatttcgc actcatcagc tgagcgtatc atcacaccag acacaactat cttgattgac
     1501 tacatgctca accgttttgg caatatcgtt aagaacttga ctgtcttccc ggaaaatatg
     1561 atgcgcaata tggaatcaac ttttggtttg atttatagtc aacgtgttat gctcaaattg
     1621 attgaaaaag gaatgacacg agaagaagct tatgacttag ttcaacctaa gacagcttat
     1681 tcctgggaca atcaagtgga tttcaaacca cttttagaag aagacaccaa agttacctct
     1741 tgtcttacac aggaagaaat tgatgaacta tttaatccga tttattacac aaaacgtgtt
     1801 gatgatattt ttgaaagact aggattagaa aaataaaaaa attccattac tggttaacgt
     1861 ttttttaacc agtaatggaa tttttattga tttaaaagga attaatatta aaaaaacata
     1921 cttaaaatga gtttattatg ataatatata ataagaagaa aaatttagga gttagttttt
     1981 aaaggaaaat gttaaaacat tttggtagta aagtaagaaa tttgagagta actagaaata
     2041 ttactcgcga agatttttgt ggtgatgaaa ctgagttatc tgtaagacaa ttagctagga
     2101 ttgaaagtgg acagtcaatc ccaaacttga ctaaagctca ctacatagct aaacaat
//
LOCUS       DATRNX010000162         2093 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRNX010000162 DATRNX010000000
VERSION     DATRNX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.164"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            409..1262
                     /locus_tag="VBC81_001931"
                     /pseudo
     CDS             409..1262
                     /locus_tag="VBC81_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
     gene            1335..>2093
                     /locus_tag="VBC81_001932"
     CDS             1335..>2093
                     /locus_tag="VBC81_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912931.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYAT"
BASE COUNT          703 a          280 c          374 g          736 t
ORIGIN      
        1 gttcacacca tggggtgttt tttgtgtaat attcaaaaat tgtcctaaag tcatttttaa
       61 aacttctaaa tacttgagaa accattgtaa atttaaataa ctactttaaa tagcacaaag
      121 gcaatgacaa agttagatga gatgattcta ttttaattta taattgaaaa aaagagtata
      181 tgatggagga attttgtatg gtaagtactc aatatatgaa agtaacctta cgagaaaaat
      241 tcccagataa attaacatat gttgatttag aagaacttgt gatatgatat tacaaataaa
      301 agaaaatgac tatatatcag ctcatatatt ggttaaagtt ggtaaatata aattattgaa
      361 attacctcat aagtttacca atgagattac ggttagaaag gaatactaat gtttatatat
      421 ggccctattt ttagggaatt tcgacttaat cgacagtttt ctcttaaaca ggttgcttca
      481 aatgaacttt ctgtttctca gttatcacga tttgagaggg gtgaatcaga tttatcatta
      541 acaaaatttt taggggcttt agaggcaatt gatttatcaa ttagcgaatt tatggataga
      601 gtaaataaat atcaaaaatc tgatcagtaa tgtctcagat ggctcaatat cattatcaaa
      661 gagacgtagc tgggttagaa aagatgattt ctgtggaaga aggtaaacta aaaaaggata
      721 gttcagatat acgttgtcgt ttaaacatag ttctttttag aggaatgata tgtgaatatg
      781 actcctcaag aaaaatgtcg gaagaagacc tttgcttttt gtcggattac ctctttcaaa
      841 aggatagttg ggaaattagt gactatattt tgattggaaa tctttaccgg tattacaaca
      901 caaggcatat ttgccaactt gttaaagagg ttatcaatca aaaggaatat tatcgagata
      961 tttatacaaa ccgcaatgtt gtcgaagcaa ccctcctaaa tgtggtagag acactaatag
     1021 aacggcgcgc tctagaagaa gcaacgtttt ttctagagaa agttgaagca cttttaaata
     1081 atgagcggaa tgcttatcac cgtattattt tactctatga aaaaggattt cttgcctatg
     1141 ctaaagggga tagtcgagga atacaaagca tgaagcaagc tattttttgc ttccaagcaa
     1201 ttggttctaa acatcatgtt gaaaattttc aggaacactt taatagggta acaagattat
     1261 aaaaaatccc atttatggga ttttttaata tttctccttt tggtttgcta taatgtcgaa
     1321 ctaaggaggg aattatgaat attaatggta taaaattact atctagtcga gcagtcagta
     1381 aacttggtga tgtgctttat gattatggaa atagtacttg gattgcttcg ataggtggtc
     1441 taggacaaaa gattttaggt atttatcaga tagtagagtt gttggtttct attgtcctca
     1501 acccttttgg aggcgcctta gctgatcgct ttcaacgaag aaaaatttta ctaataacag
     1561 atgctatctg tgctatcatg tgtttcttac tttcttttat tggagatgat aaagtaatgg
     1621 tgtatggtct aatagttgct aatgctatct tagcggtttc caatgctttt tcttctccgg
     1681 cttataaatc ttatattcct gaaatagtag ataaagctga tatcattacc tataatgcta
     1741 atctggaaac cattgtgcaa attattagtg taagttcccc tgttttaggt ttccttattt
     1801 ttaataattt tggtattagg attactttaa ttgttgatgc gattactttt ttgatctctt
     1861 tcctgtttct ttatgctatt aaagtggaaa gggtacagct ttcaaaacaa gaaaaggtag
     1921 cgataaaaaa tattttagct gatattgcag atggatttac ttacatcaaa aaagaaaaag
     1981 aaattatgtt ttttctaatt attgctgctt tattaaacac tttcctagca atgttcaatt
     2041 atcttttacc ttttactaac tccctattga aaacttcagg tgcttatgca aca
//
LOCUS       DATRNX010000163         2081 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRNX010000163 DATRNX010000000
VERSION     DATRNX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1326
                     /gene="rlmD"
                     /locus_tag="VBC81_001933"
     CDS             <1..1326
                     /gene="rlmD"
                     /locus_tag="VBC81_001933"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6912932.1"
                     /translation="LKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFCQISSVRRNFA
                     EAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQALMKFKPEGY
                     ENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIKDCLVQDRLTQ
                     EMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSKRLDFDDVVIE
                     LVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSLSPRAFYQLNP
                     KQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDIIPEAIQDAKE
                     NALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLLNTILKMPPEK
                     MVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRKV"
     gene            1594..1845
                     /locus_tag="VBC81_001934"
     CDS             1594..1845
                     /locus_tag="VBC81_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912933.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            1842..>2081
                     /locus_tag="VBC81_001935"
     CDS             1842..>2081
                     /locus_tag="VBC81_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912934.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFG"
BASE COUNT          721 a          278 c          426 g          656 t
ORIGIN      
        1 ttaaaaatta aacgaatggg aattaatggt gaaggaatag gtttttataa aaaaacacta
       61 atctttgttc caggggctct caagggagaa gaggtgtttt gtcagatttc ttctgttaga
      121 cgtaattttg cagaagctaa actgttaaag ataaataaaa agtctaaaaa tagggtagat
      181 cccgcttgtt ctatttataa agaatgtggg ggatgccaaa taatgcacct tcaatatgat
      241 aagcagctag agtttaaaac ggatattatt cgacaggctc taatgaaatt taaaccagag
      301 ggatatgaga attatgaaat ccgtaaaacg ataggtatgt cggaacctga acactatcgt
      361 gctaaattac aatttcaagt gcgttcgttt ggagggaatg taaaggcagg tttatatgca
      421 caagggacac atcgattgat tgatatcaaa gattgtttag tgcaagatag gcttactcag
      481 gaaatgataa atcgagttgc agaactttta ggaaaatata aattgcctat ttataatgaa
      541 cgtaaaatag ctggtgttcg tactgttatg attcgacgag cacaagcgag tggagaggtt
      601 caactgattt ttatcacaag taaaagactt gattttgatg atgttgttat tgaattagtt
      661 cgagagtttc ctgagttaaa gacagttgct gtaaatatca acgctagtaa aacaagtgat
      721 atctatggtc aaataacaga agttatttgg ggtcaagaat ccatcaatga agaagtatta
      781 gattatggtt tttcgttatc accacgtgct ttttatcaac ttaatcctaa acaaacacaa
      841 attttatata gtgaggcagt caaggcctta gatgtgaagg aagatgatga tttaattgac
      901 gcttattgtg gagtaggaac gattggactt gcttttgctg gaaaagttaa aagtgttcgt
      961 ggaatggata ttatccctga ggctattcag gatgctaaag agaacgcact tcatatgggt
     1021 tttactaata cacattacga agctggaaag gcagaggata ttatccctag gtggtatagt
     1081 gagggatttc gcgctaatgc gttaatcgtg gatcctcctc gcacaggctt agatgataaa
     1141 ttgctaaata ctatactaaa aatgcctcca gaaaaaatgg tttacgtttc ttgtaataca
     1201 tctacgcttg ctagagactt agtaacatta acaaaagtat accatgttca ctacatccaa
     1261 tctgttgaca tgtttccaca tacagcaagg accgaggctg tagtgaaact acaacgtaag
     1321 gtataataaa gttttctaat ctaaaaataa gagtattttt gggtattctt atttttagat
     1381 agtaatggtg agatattaat atagcatagt ataaaacaca tggtttaagc tatttgatac
     1441 ggaaatttac cttagaaatg gtaaataatt ccattaagta tattcatctt tgcaaatttg
     1501 accttgcctt ttaagtttca ttgggttaaa atagaaaaaa tatttttaac aagatgatag
     1561 aacatcgtaa attaaggttt aaaggaaatg cttatgataa taaattcatt cgataatagt
     1621 agaaaagcga ctattaatcc tgaagatatt aattcaccaa taaaaggttt tccaaagact
     1681 gttattacct gttttgcgcg ggagactttc aatcgcattt tagaagaatt gcctcatcgt
     1741 gaaattgcta gaactagtgt agcaaactta gaaattccca tttatgaatt agaatttaaa
     1801 ggacaaaaga ttgtccttat caagaggttg gtgatgaaga tttgatttgt ttgcatggga
     1861 tgaattgggt agatggtcaa ttggcagctt attatagatt gataccagaa gatgataagg
     1921 tgcatttggg acgtgtgatt gttaatccag actttagaaa aaagggatta ggaaatcaat
     1981 tagtggaata tgagattaaa tttagcgagg caaattaccc taataaacct atttatgctc
     2041 aggcccaagc ttatttacaa gatttttatc aatctttcgg a
//
LOCUS       DATRNX010000164         2045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.166, whole genome shotgun sequence.
ACCESSION   DATRNX010000164 DATRNX010000000
VERSION     DATRNX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..170)
                     /locus_tag="VBC81_001936"
     CDS             complement(<1..170)
                     /locus_tag="VBC81_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6912935.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHV"
     gene            170..574
                     /locus_tag="VBC81_001937"
     CDS             170..574
                     /locus_tag="VBC81_001937"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6912936.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            736..936
                     /locus_tag="VBC81_001938"
     CDS             736..936
                     /locus_tag="VBC81_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6912937.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            958..1218
                     /locus_tag="VBC81_001939"
     CDS             958..1218
                     /locus_tag="VBC81_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6912938.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            1344..1553
                     /locus_tag="VBC81_001940"
     CDS             1344..1553
                     /locus_tag="VBC81_001940"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6912939.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(1726..1887)
                     /locus_tag="VBC81_001941"
     CDS             complement(1726..1887)
                     /locus_tag="VBC81_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO6912940.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT          689 a          358 c          386 g          612 t
ORIGIN      
        1 acatgtatgt taaaacgctt tactgtatct tgcacatgcg tttgactata atgatttatg
       61 acttgttgat atttagtgga agtaatattg caaagtaata tatttcctat tatatgttta
      121 tacgatattc gatattccca cccgttgtcg cgtttacgga aatacgccat tgatatactc
      181 cacattagct aaagaacagg gtgttcaagg ctaccttgat ggaaaaggct ctcttagaga
      241 tatttgtaaa tggtatgata tctcaagtcg ctctgttctc caaaagtgga taaaacggta
      301 tactagtggt gaagacttga aagccactag tagaggatat agccgtatga aacaaggaag
      361 gcaagccaca tttgaagaac gtgtagagat tgttaactac accattgccc atgggaaaga
      421 ctatcaagca gctattgaga agtttggtgt ttcctaccaa caaatttatt cttgggtgcg
      481 taagcttgag aagaatggct cacaaggttt ggttgataga cgtgtgaaag ggttggagag
      541 taggcctgat ttaaccgaga ttgagcaact ttaactcaag attaaacaat tggaggaacg
      601 taatcgtctc ttagaaatcg aggttagttt actaaaaaag ttagaagaca tcaaacgagg
      661 aaacagacgg taagactagg taagcattta gcggagttcc aagtaatcaa gaattattac
      721 gatgaggaat ctaatgtgcc tattcaggcc ttatgccaac tcttgaaggg gtctcgttca
      781 ggctattaca agtggctcaa tcgtcaaaaa acagattttg agacaaaaaa tacaaagcta
      841 atggctaaaa tcaaggaact tcatagactc tacaatggta tcttaggtta tcgccgtatg
      901 acaacattta ttaatcgtca acttgggaca acttaaaaca agaaacggat tcgttgattg
      961 atgaacattc tggggattag ttcagtcatt cgtcgtgtta gccatgcttg tacaaaagct
     1021 ggtgacagat tttacgaaga aaatattctt aatcgtgaat ttacagccac agctcataac
     1081 cagaaatggt gcacagatgt cacctatctt caatacggtc tgggagctaa agcttatctc
     1141 agtgcgatta aagacctgta taacggttct attatcgctt atgagattag tcacaacaat
     1201 gaaatccact tgttatgaag accattaaaa aggggctaga gctcaatcca ggagccacac
     1261 ctatcatcca tagcgattga ggtagtcaat atacttccaa agaataccgt tatatcatac
     1321 aacaagctgg tctgacctta tccatgtccc ggattggcaa atgtattgat aatgcaccaa
     1381 ctgaaagttt ctttgggttt ttcaagactg agtcttacca ccttaagaaa tacaactctt
     1441 atgatgagtt ggtcaatgat gtggcacgtt atatcgaatt ctacaacaca caacgttatc
     1501 aatcaaaatt aaacaatctg actcctctag aattcaggaa tcaggttgca taacttatct
     1561 tttattattt gactgtctac ttgacaggga gccgttcaga ttgcttaacc tttctaaatt
     1621 tgctaaaata gctacaagaa aacgagccat ttaatgctta tttcttatac tgtcttgcct
     1681 cacgctctcc tcgaccaaaa attgagcgtg aggctttttg tttcattaaa cgatgatatt
     1741 tccatattca tcagtttgtt ttccgagagc catcaaagct tcgataaggt cgataattcc
     1801 aggaataaag gtaatactaa aaataatata taaaaaaacc tggcctattt ttcctgcgta
     1861 aaatttatgc gctccaatgc cgcccaaaag aacgttaata aaacataaac tactatgtta
     1921 gcataagact ttatttttac aactgaattt catataaatg gattagagta agggataaaa
     1981 gaaattagca tagctctttt gaaaataaaa aaattaatat aatatggaaa aaattttatt
     2041 tcata
//
LOCUS       DATRNX010000165         1981 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRNX010000165 DATRNX010000000
VERSION     DATRNX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..149
                     /locus_tag="VBC81_001942"
     CDS             <1..149
                     /locus_tag="VBC81_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6912941.1"
                     /translation="GALIIISLVKLSKLKMTKHLSKPVWFLVIIFLNIIGPIAFLILE
                     GNNE"
     gene            146..1048
                     /locus_tag="VBC81_001943"
     CDS             146..1048
                     /locus_tag="VBC81_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912942.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            1045..1806
                     /locus_tag="VBC81_001944"
     CDS             1045..1806
                     /locus_tag="VBC81_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912943.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
BASE COUNT          685 a          271 c          337 g          688 t
ORIGIN      
        1 aaggagcttt aattatcatt tctctagtca aactaagtaa acttaagatg actaaacacc
       61 tttcaaagcc agtttggttt ttggtcatta tatttcttaa tataattggg ccaattgcat
      121 ttcttatttt ggaagggaat aatgaatgaa agctatctca tttcaaaacg ttacaaaatc
      181 ttttggtcct aaaaaaatat taaataatgt ttcttttgat ttagaagaaa atatgattta
      241 tggattcgta ggaccaaatg gagcaggtaa aacaacgaca ataaaaatga ttttaggatt
      301 gttaaaattt gatacgggtt tcataacaat ttttggtaaa aaagttaatt ttggaagaac
      361 agataccaat caactgattg gctatctccc agatgttccc gagtactacg attatatgac
      421 agctctagaa tatcttgatt tatgttctgg tttagcgaga tcaaagcata agctttctaa
      481 caaagaacta cttaggtcag ttggacttga cgataatcac caaaagattg ctacttattc
      541 tcgaggaatg aaacaacgtc ttggattagc ccaagcactg gtccatgatc ctaaaattat
      601 tatttgcgat gaaccaacct ctgcattaga cccgaaaggt cgccaagata ttttagatat
      661 tatttcaaat cttagaggag aaaaaactgt gattttttct acccatattc tctctgatgt
      721 cgaaaaaata tgtgatcacg ttctggtgtt aactaagtgt gggatttatt ctcttgaaga
      781 gttaaaagga aaaaaatcag aggaaaatta ttcggttcgt attttaataa aagtgacaaa
      841 gtctgaagct aaggtactat ctcataacta tcagatagaa aagaaagata atgagtatgc
      901 actgactctc aaaggctcta aaatggataa caaagcagat ttactagctg gtttttacca
      961 agatttagtt tctttaaaga ttagcccatc agctattgaa gtcattgaca atagtttaga
     1021 ggagctttat ttggaggtta ccaaatgaaa tctttagcag taatgttaaa aaaagaatgg
     1081 atggagaatg ttaggacata caaagttata tctattctga ttacttgttc aatatttggt
     1141 atattaggtc ccttaactgc acttatgatg cctgatatta tggcaggaat tttacctaaa
     1201 aaactacagg gagcgatacc agagactacc tatattgatt cttatattca gtattttaaa
     1261 aatatgaatc agcttggttt agttatttta gtatttttat ttagtagtac actgacgcaa
     1321 gaattttcaa aaggaacctt aattaatcta gttacgaagg gattagccaa aaaagttata
     1381 atccttgcta aatttatagt aattaccctt ctatggacag tgtcatactt attaagtgtt
     1441 gtcatccatt ttagttatac gttatattac tttagtaacg aaggaagtca taaattaatg
     1501 gtttacggtg caacatggtt tatcggtatt cttttcataa gcttaatact ctttttttca
     1561 gttctttttc gtaaaacttt agggattttg ttaggttgtc tttttatggt ggtgttatgt
     1621 gtagtatctg gattttttaa aatcagtaaa agctataatc ctatgcttct tatccaaaaa
     1681 caaggagaaa tgttaagtgg aaagtttaaa gttcaagact taatggaacc gagcatatta
     1741 gtgtttttgc ttattataat gacgttatta cttagcatgt acatttttga aaaaagtgaa
     1801 gtttaatttt gataagatat caataaggcg atagctactt tttgaaaaaa taaaaccgaa
     1861 ttaagagctt tgctgctatt tgacttatct agatcaatat ttccaactaa tatgatatcc
     1921 tatgcatatc tcattgcaga aatatacttt aaagtcaaaa taaggaaata tattatggaa
     1981 a
//
LOCUS       DATRNX010000166         1926 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRNX010000166 DATRNX010000000
VERSION     DATRNX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1192
                     /locus_tag="VBC81_001945"
     CDS             <1..1192
                     /locus_tag="VBC81_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6912944.1"
                     /translation="ATTLYGFKSTIDLTNNLNKTASYSEIEMSVVVPKDSKITNIEAV
                     SKLAAPVKNDTSNITDLIEHIKSEKGISITPQKTDSYQDAYNRIKNGDSQAMVLNNAY
                     VSLIELSTPDFKSQIKTIYTYKIKKKINRKNTNHKEGVFNIYISGIDTFGSISTVSRS
                     DVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQYDKLTHAGLYGVETSMKTLENLY
                     DINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSKHGNFDFPVGQVTLNSEQALGFV
                     RERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTKLNSITSQLQTSVQTNMTIDNIN
                     DLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPGAQLYMMSIDQSSLSNAKSKIKN
                     TMEE"
     gene            1198..>1926
                     /locus_tag="VBC81_001946"
     CDS             1198..>1926
                     /locus_tag="VBC81_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912945.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
BASE COUNT          723 a          287 c          319 g          597 t
ORIGIN      
        1 tgctactact ttatatggat ttaagtcaac cattgatttg acaaataatc taaataaaac
       61 tgcttcatac tctgaaattg agatgagtgt agttgtacca aaagattcta aaataaccaa
      121 tatagaagct gtcagcaaat tagccgcacc agttaaaaac gatacttcaa atattactga
      181 tttgatagaa catataaaat cagaaaaagg aatctctatt acaccacaaa aaacagattc
      241 ttaccaggat gcatacaata gaattaaaaa tggtgatagt caagctatgg ttttaaataa
      301 tgcttatgtt agcttaattg aacttagtac ccctgatttt aaatcgcaga taaaaacgat
      361 ttatacttac aaaattaaga aaaaaattaa tcgtaaaaat actaatcata aagaaggggt
      421 atttaatatc tatattagcg gtattgatac ttttggctct atatcaacag tatcaagatc
      481 tgatgtaaat attattatga cggttaatac caatacccac aaagtattgt taacgacaac
      541 accacgagat gcctatgtaa aaattccaga tggtgggggc aatcaatatg ataaattaac
      601 ccatgcaggt ttgtatggtg ttgagacatc aatgaaaaca cttgaaaacc tttacgacat
      661 caaccttgat tattatgcta gaattaattt ttcatcattt ttaaaattaa tagacctctt
      721 gggaggagtg acagtttata acgatcaagc ttttacaagt aaacatggta attttgactt
      781 tcctgttggt caagtaacat tgaattctga gcaggctttg ggctttgtta gagaacgtta
      841 ttctctacaa ggaggcgata acgatagagg tagaaatcaa gaaaaagtga ttgcagctat
      901 tataaataag ttagcttcta gtcagtcagt aacaaaatta aatagcatta cctcacagct
      961 ccaaacgtcc gttcaaacta atatgactat tgataatatt aatgatttga ttaacaatca
     1021 attgtcaact ggacaacgct tcactgtcga gtcacaagca ttaactggtc atggttcaac
     1081 gggtgaactc ccttcatatg caatgccagg agctcaactt tatatgatgt caattgatca
     1141 atctagctta tctaatgcaa aatcaaaaat taagaacaca atggaggaat aacaagaatg
     1201 attgatattc attctcatat cgtttttgat gtagatgatg ggcccaaaac gcttgaagag
     1261 agtttatctt taatagaaga aagttatcga caaggtgtta gaataattgt ttcgacatca
     1321 catagaagaa aaggtatgtt tgaaacacct gaagatatta tcttcaaaaa cttttctata
     1381 gttaaacacg aagcggaaaa aagatttgaa catcttcaaa tcttatatgg tggagaatta
     1441 tactatacaa gtgatatgtt agaaaaactg aagttaaagc aaattccaac tttaaacaat
     1501 acgaaatttg ctttaattga attttctatg caaacttctt ggaaagatat tcatacagct
     1561 ttgtcaaatg ttttaatgct tggtattaca ccagtcgttg cgcatataga gaggtataac
     1621 gctttagaga atcaaaaaga acgggtgaag gaaattatta atatggggtg ttacacacaa
     1681 ataaatagtt tccatatttt gaaacaaaaa ctttttaatg ataagcataa acgctttaag
     1741 aaaagagccc gttatttttt agaggaaaat ttagtgcatt ttgtagcgag tgatatgcat
     1801 aaccttgatg ttagaccgcc atttttagca gaagcttata agattatctg tagagatttc
     1861 ggtaaagaac gtgctaacca actttttatt gaaaatgctc aatctatatt aaaaaaccat
     1921 tacatt
//
LOCUS       DATRNX010000167         1905 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRNX010000167 DATRNX010000000
VERSION     DATRNX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..249)
                     /locus_tag="VBC81_001947"
     CDS             complement(<1..249)
                     /locus_tag="VBC81_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6912946.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVG"
     gene            complement(339..923)
                     /locus_tag="VBC81_001948"
     CDS             complement(339..923)
                     /locus_tag="VBC81_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912947.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            1012..1824
                     /locus_tag="VBC81_001949"
     CDS             1012..1824
                     /locus_tag="VBC81_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6912948.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
BASE COUNT          738 a          341 c          259 g          567 t
ORIGIN      
        1 tccgacaaaa tgcatatgaa tgatatccgt caacatataa aaggtattag gatagaaaaa
       61 aaaccaagca agtgcaaaaa gaccgataag aatttttttc ttaaataaac tagtaaggta
      121 tgcaaaatct aacgctatta gggctagagt catattccaa atcaaatttg gtccctgcaa
      181 atgataatat ttaatcccaa aagaaataac aataaaaaat aaatgtatcg caaatagttt
      241 tactttcatt ctaacctcca attaatatct cattatacaa catattatta aattaaggtt
      301 aaataaactt caaacctaaa agactaaccc tgttgtggtt agtcttccat taatattccc
      361 caaagtaata attgtactaa aaaaaggcaa gacaaggctt taaaatcaag gttagccata
      421 ttagtctgta ttccattaaa aaatgctgac ggatttaaaa tgattccttc taaacctaat
      481 gatcctgatt tttctaagca aaaggcaaaa ctactaaaaa gcattacagg tataacagtt
      541 gctaattgat accttttact aatatcatat gcatttacca atagcttcaa acttagtaaa
      601 gctgaagaga taataaaaag aattgcaaga aggtatgcca tatattgcat aaagtgatta
      661 gactgccaaa tgtcttttgt tgaccactct aagctcacta aactaagaaa aatccctaaa
      721 ctctgtgggt aaaaaaataa aaggcctaaa cttgctaaaa gacgaataaa aatattacga
      781 ctataataag caatgaatcc catctctaac gacaagactg atgccaatag aattaccatc
      841 aaatgatcaa tttttacatg ataataccat atccctaaaa tactgactat ggcaaaaaga
      901 tgactccaaa ttaacttttt cataatccac caacttccat atttccacag ttattatatc
      961 atatttctac tgtctctttt tattgaaatc tatttttgat ataatgtcag catgacaaag
     1021 aaaattatcg ccgtagattt agacggtact ctattacata ataataacac tatttctgac
     1081 tacaccgctg atacacttcg aaaagttcaa gctcaaggac acaaagtaat tattacaacc
     1141 ggacgcccat accgtatggc actcgctcat tatttacgcc tagatttaaa aacaccaatg
     1201 ataaatttta acggtgcttt aactcatatc cccgaaaaaa agtgggcgtt tgagcatagt
     1261 gcaacgattg ataaaaaatt actccttgaa accttaaatt tatcagatgc tatccaagcc
     1321 gattttattg ctagcgagta ccgtaaaaat ttttacatta ctatggataa tcgtgataaa
     1381 attaacccac aattattcgg tgtcaatgaa attactgata aaatggcatt agatgttact
     1441 aaaatcacac gcaatccaaa tgctctattg atgcaaacac gtcataaaga taagtatgag
     1501 ttagctaaag agttacgaca acattttaat cacgaactag aagtagattc ttggggtggt
     1561 ccattgaata tactcgaatt ttcacctaaa ggtgttaaca aagcttatgc tctcaaacat
     1621 ttattaaaat cactcaatct ttcacaagaa aatttgattg cctttggaga tgaacacaat
     1681 gacaccgaga tgctggcctt tgcacataca ggatatgcta tgaaaaatgc tagtccaact
     1741 ctacttccat atgccgacca acaaatccaa tggacaaacg aagaagatgg tgttgctaaa
     1801 actctagaaa aattattatt gtagtatttt caaaaacgat ctctattgaa acagaattct
     1861 aatagaaatc gttttttagg ttttctacta tattattaac aaaaa
//
LOCUS       DATRNX010000168         1898 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.170, whole genome shotgun sequence.
ACCESSION   DATRNX010000168 DATRNX010000000
VERSION     DATRNX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.170"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..>1898)
                     /locus_tag="VBC81_001950"
     CDS             complement(<1..>1898)
                     /locus_tag="VBC81_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6912949.1"
                     /translation="SFLLPFIIIVCILFTKNIYWGSPTTILASDSFHQYVIFNQALRN
                     ILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKNMPDAIYLLTICKVGL
                     IGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMWLDVFILIPLVVLGVD
                     QLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITRNTDSKVAFKQFLHFT
                     FLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMDLFAKNMIGAYDTTKF
                     GSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIASFYITPLDLFWQGMHA
                     PNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILISGFIITFLFKKHYHY
                     LNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVLEMTLNTFYQLNSLND
                     EWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGNDSMKYNYNGISQFSS
                     IRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNLTEYPFDKFGFIRKAQ
                     DKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQLSGLKETYFEHLIPNS
                     VSGQTTLN"
BASE COUNT          722 a          276 c          316 g          584 t
ORIGIN      
        1 tattaagagt agtttgccca gaaacgctat ttggaataag atgctcaaaa tacgtctctt
       61 tcaaaccact taattgattt aaaagagttg tctgattatc caaagtattc acagtaaatg
      121 gtttatcttg atatacttga ttcgttagta tagctaattg actggcaaac tgattttcgt
      181 aaagaatggt ttgtttatct tgagcttttc tgataaaacc aaatttatca aaaggatatt
      241 ctgtaaggtt gtactttaca ccaaatagac tatctgcgat caaagtatta ttttgataac
      301 gtaaatttaa atttgtacca tctgatttaa agccaagtct atcaagcact tgacttgaag
      361 aacgatttct aatagatgaa aattgtgaaa taccattata attatatttc atagagtcgt
      421 tgccggtttg tcctaaccag cgctctgtac ggaagaatgt agagttattt ctctcagtct
      481 ttctgacaag tttgctaatg ctgtgattat atttagcata tccttgccta gatggaaata
      541 cccactcatc gttaagacta tttagttggt aaaaggtgtt taatgtcatc tctaacacaa
      601 caaaaccaat aagaaaggga taaaaaacta atttagggat ttgcttactg acaaaactaa
      661 tagttagaat aatataagct gagaggaatg ttaaggttaa aagaagtaat tcaagattaa
      721 ggtaatgata gtgcttttta aataaaaacg tgattatgaa accacttatt aaaattaaat
      781 tgacccctaa aatctttttc caagaaatgt tgtctaaata ctcaaggcat tcagcagcta
      841 gtagacagat aagtactgaa agtacccaag agtagcgatg aagaaacata tttggagcat
      901 gcatcccttg ccaaaataaa tctagaggtg ttatataaaa actagcaata acaaatatta
      961 gtgaaaatcc atatgctaat ctagtccttc gaggaatttc ctttattgta aagtaaagta
     1021 aagaaaggag taaaggaagt aaacctacat aaatcatagg aatagagcca aattttgttg
     1081 tatcataggc tccaatcata tttttagcaa acaaatccat gtaccaagaa ttttctgtaa
     1141 acatcttcga tacctttgtc aacttttcac catgagtagt taaatcaaaa taagttggca
     1201 aaatcataat gctactagtc ataccagcta aaagagataa aaaggtaaaa tgaagaaatt
     1261 gtttaaatgc aaccttacta tcagtattac gggttatttg tacgatgaag taaaggctag
     1321 taaagatggt agtcataaaa ccaaaataat agttttggat gaataaggct gtcagagaga
     1381 gaaaatgaag tataggtttt ctttcccata gtagctgatc aactcctaaa acaacaaggg
     1441 gaattagaat aaatacatct agccacatat taatttcaat ttggctaatt gagaaactca
     1501 ttaagctata gcaagtagaa attactaaaa gtaagacacg attaacttta caatgacgtt
     1561 tacaaagagt gacaaacatt gacaaaccta tcaaccctac tttacatatt gttaaaaggt
     1621 agatagcatc aggcatattt tttaggttaa agaaataaac tattggggat aaaaaacttc
     1681 ccaaatagta actactcaga gcataaaaat ttaaacctaa accgcttgta aaggtataga
     1741 ataagctgtt tgaaccatga agaatatttc ttaatgcttg attaaagata acatattgat
     1801 gaaagctatc gcttgctaat atggttgttg gacttcccca ataaatattc ttagtaaaga
     1861 ggatacagac tatgattatg aagggaagta gaaagctt
//
LOCUS       DATRNX010000169         1814 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRNX010000169 DATRNX010000000
VERSION     DATRNX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            62..868
                     /locus_tag="VBC81_001951"
     CDS             62..868
                     /locus_tag="VBC81_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6912950.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            951..1454
                     /locus_tag="VBC81_001952"
     CDS             951..1454
                     /locus_tag="VBC81_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6912951.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1558..1734
                     /locus_tag="VBC81_001953"
     CDS             1558..1734
                     /locus_tag="VBC81_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6912952.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          660 a          253 c          356 g          545 t
ORIGIN      
        1 cttgacttat gtataaagat gatttatact tgttcctatc ataataaaag gaagatagtt
       61 tatgtcaaaa aaaataatat taggaatttt atctctttta tctgtcgtta ctttggtggc
      121 gtgtggttca tcagacaaac agctacaaga taaagttgag aaaaaaggga agttagtttt
      181 agcggtgagt ccagattatg ctccctttga gtttaaagcg cttgttaatg ggaaagataa
      241 agttgtcggc gcagatattg agttagctca agctattgct gatgagcttc atgtcaaact
      301 agaggtatct cctatgagtt ttgacaatgt tttatcaagt ttacaaacag gtaaggctga
      361 tatggctatt tctggattat catatactaa agagcgtgct aaagtctatg atttttcaaa
      421 accttattac actacagaaa atgcggttct tgttaggaag tctgatttag gaaaatatac
      481 ttcaatcgaa agccttaaag gtaaaaaaat agcagtacaa aaaggaagta tcgaagaggg
      541 agtttctaag aaatcattca aaaattctca cgtcgtgtca ctgacagcga tgggagaagc
      601 gattagtgag ttaaaatcag gcaaggtaga agctgttgat ttagaaaaac ccgtagctga
      661 aggttacttg gcccagaatc cagaccttgt tcttgctaag tttgccctta aaacaggaga
      721 aggggatgct aaggctgtag ctttacctaa agacagtgga aaaatgttga agactgttaa
      781 caaagtgata aagagacttg aaaaagaaga taaatataaa gtttatattt cagatgcggc
      841 taaattaact ggcaatcaag ttgaataata ttaaaaagcc tagtcatagg cttttttgga
      901 tgttataata tggcttgact aagaataata gcataaaggc agaggaatat atggttcagg
      961 gttcaattaa tattgaaaaa tataaagatt tggcgctcca aaaacaaggc gagcatcgta
     1021 aatttttagc aagtttaaaa aagaaagctc ctaaaaactt agataaaata gtccaacaga
     1081 tacatcaaga ggtttttcaa gaaattgatt gtacagcatg tgctaattgt tgtaaaactt
     1141 tgggtcctct atttacagaa gctgatattt ctaggatttc taaacatttc cggatgaagt
     1201 tatcggcttt tgaagattta tttttagaag tagatgaaga tggagataaa gtatttaaag
     1261 cgatgccttg tccgttttta ggcgatgata acttatgtag tatatacgat gtcagaccta
     1321 aagcttgtcg tgaatttcca catactgatc gtaagaaaat ctatcaaatt aataacttaa
     1381 cgataaaaaa tactcttact tgtccagcgg catacctttt tgtggaaaaa ttgaaatcac
     1441 gtatagggaa ataataaccc agagaaagta tcataaaaat attgacaatc tgcttttatt
     1501 tgggtatcct agtaagtgaa cgtgaaaatg ttacaatatc ttgaggaggt gaaacatatg
     1561 tcaaaaacag tagtacgtaa aaacgaatca cttgacgatg cacttcgtcg tttcaaacgt
     1621 tctgtgacta aagctggtac tcttcaagaa tcacgtaaac gtgaattcta cgaaaaacct
     1681 tctgtaaaac gtaaacgtaa atcagaagca gctcgcaaac gtaaaaaatt ctaattagat
     1741 agaatatgaa aaaacagttc agaattgagc tgttttttca tatattataa ctataagtta
     1801 ataaaaaaac tcgg
//
LOCUS       DATRNX010000170         1730 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRNX010000170 DATRNX010000000
VERSION     DATRNX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1014)
                     /locus_tag="VBC81_001954"
     CDS             complement(<1..1014)
                     /locus_tag="VBC81_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEO6912953.1"
                     /translation="MLKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQL
                     KSVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNN
                     LSGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAK
                     ASVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAF
                     IIIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVV
                     AWLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILG
                     AFLI"
     gene            complement(995..>1730)
                     /locus_tag="VBC81_001955"
     CDS             complement(995..>1730)
                     /locus_tag="VBC81_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6912954.1"
                     /translation="NWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAYFSK
                     YYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLALVF
                     QTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVISLM
                     LEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHIIKQ
                     DSHVFNIIAKKFINDTLKGEIVEKAN"
BASE COUNT          649 a          348 c          245 g          488 t
ORIGIN      
        1 tattaagaat gcacctaaaa tacgcatcaa atggctcacc atggtttgta gaacagttga
       61 tactaactct ttcgggacat tttcataacg ttgatttatt tgactaccta aataatggat
      121 aaagaaatag atacctgcaa agaatggaat aatatagtag gctaaacgat aaagtaataa
      181 ccatgcaacc acagtttctt taggtagtcc ctcggcagca aaccctgtaa atagaactaa
      241 ttcaaaactt cctaatccac cgggaataag ggatacaatc ccgacggcac aaccaataca
      301 aaatagtggt aaaattttat aaactggtag atgaatgccc attaaataac cgataattat
      361 aaacgctgcg gctgcacatc cccattcaaa aaaagaaaca atacctaatt ttatacgagt
      421 actagatggc atatctccga aataatggct tcctttatga ccagaaatcc aataaataac
      481 aggaaaatac atactagcac caattaatac caaataatag taatcaacac tagctttggc
      541 atgaaaaata tggctcatga ttaaggcaat cacactaata aatgacagac cagaaataag
      601 atagggtaaa aaacgagtca cttctcttag gtcactcttc tcttgacctt ttttaccata
      661 aaaagccatg cgcaacccaa tatcgattaa gccaccgaat cctgacaagt tattaaaagt
      721 gttgatacac caactagtct gtaaaatata ccttttgctt ttatctgtac gtaaaagtcc
      781 attcagtaca aagtcatatc cggttgtcgg taagacagcg ataaccccca caaggataat
      841 taagaaaaga ttcattggag ataattgccc aaaaacactc tttagttgct caacagaaat
      901 agttcgtttc aaatgaatca tttcaacaat aataagcact aaaacagata taaaaaatac
      961 aattttaatc acagaagtca gtgatttgac tttttcaatt agctttttca acaatttctc
     1021 ctttcaacgt atcgttgata aacttttttg caataatatt aaaaacatgg gaatcttgct
     1081 taataatgtg atgcccaaag ccaactaaag aataaaactc cccccttgga aaataagaag
     1141 caagtttctt agaatgattt aacttaatta tgtccttatt tccaaccaaa accattacag
     1201 gagttgacac atgctgtaaa tcagctggac taatcttcaa atcctccaac ataagcgaaa
     1261 taacttgagc tttttgcctc atatacggaa agagtttccc taaatagtga aggaatttat
     1321 aggcaatcct tactaaaaga atatcccacc atcgctgacc atgaatagtc aggttccctg
     1381 aattaagcaa aagccctcta accatatctg gaaacatcgt ttgaaaaact aaagctaaat
     1441 tagcaccatc gctatggcct accaatataa ctttatcaat ctctaaatga actaagatat
     1501 cctttaagtc aactgctatt tgcctgaaac taatggtatt tagctttgca tgacttttgc
     1561 catgccctct actatccata acaataactt ggtaatactt agaaaaatat gctatttgct
     1621 tatcaaaata gcgactactt aagctattgc catgtaaaaa aacaatcggt tgaccttgac
     1681 caacgacacg ataataaata tctgaaccat ctttagtatg taaccagttc
//
LOCUS       DATRNX010000171         1722 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRNX010000171 DATRNX010000000
VERSION     DATRNX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..1483)
                     /gene="gtfA"
                     /locus_tag="VBC81_001956"
     CDS             complement(<1..1483)
                     /gene="gtfA"
                     /locus_tag="VBC81_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6912955.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDICKKHEYSYRIASRFLNDKIIENWS"
     gene            complement(1499..>1722)
                     /locus_tag="VBC81_001957"
     CDS             complement(1499..>1722)
                     /locus_tag="VBC81_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912956.1"
                     /translation="DKSWIEEVDSLQQLKGVVTNRGIGQRDIIQEYYKESLNSYYKMG
                     KEIRYSIVKNIMLSTISKASDGSYSIYYV"
BASE COUNT          558 a          333 c          212 g          619 t
ORIGIN      
        1 aactccaatt ttctataatt ttatcattta aaaatctact agctattcta taagaatact
       61 cgtgtttttt acaaatatct ttagaattaa ataaagcaac aatatactga gcaaaagcag
      121 ttgatatttg ttgaggatta tctggtgttt cccttggaat aaggtagcca ttcaaattat
      181 tttcaataaa ggtctgatta ccatacggaa catctaaacc aattatcgga agccccgatc
      241 cgattgcctc catcaaggtt aaaccaaaac cttcactggt agaacccgat aaataaactt
      301 gataatcttt ataaacagaa gataggtttt tatgccccat taatcgtata taattatttg
      361 ccttattttt agcaattatt tcttgtaact tttgccgttc gccaccttca ccataaatat
      421 caaaggttaa ttcaggaatt actttattag cttctaccac agcattaatt aaccaatcaa
      481 tatgtttttc cgaagctaga cgtgagcaag ttaacaacga aaaagctttt ctttcatgcc
      541 actcttttct ctgtaatttt gataaacttc ctacaggaat tgtataaacg accggttcaa
      601 tatttaaata ctttttaaat tgatcaatca acgtcttagt ttgcgtttct gttgaggtga
      661 taaaagcatc tatgctatca gcatttgaaa attgatagtc ataataatta ttccataaaa
      721 tcgtagtttc atcagttgcg ctaacattat aatgctctgc atgaacaaca acagctactt
      781 tagcatttcc tttattcctt agaacagctt gcccaatgcc tgtagagcgg tctaacaaga
      841 ttagatcact attcgttaat tgtaattttt ctaacatgta tccaactaat tcttctttag
      901 aatatagtat agtttctttg aatttaaaaa tactattctc gccatctaca ttttcttcat
      961 atgcaacact tccatcttca ttataaaatg tacgctgata caggcatgga cctatatctt
     1021 cgggagcata atattctgaa aacattttag tatatgagta gtagtctttt ctaacaagct
     1081 ttccccccat aagatactct accctttgga cgatgttact agattcatcg ttaagcataa
     1141 ctgtaactac aatactactt cctttaaaaa aaaatgatat tattttaccg tttttctcag
     1201 tcctatcaat cggtagtgaa aaactttctt tcaattgttg cagtgaataa gaagttgcag
     1261 caattgttaa atcagtaaaa aaagtataaa gccatataat ctcattgtct tcaaagccga
     1321 tatttcgagt aaggtgctct atattttcat tctgaaacat atcagtaaaa ataaatttag
     1381 cttcaacacc taactttcta aaaacttctg aacgatatgc ttgagcatac tctacaccgc
     1441 tacttgccca acctatccct ctattcaaat tataaactac cattaactcc gttttccttt
     1501 atacataata tattgagtaa cttccatcac ttgcttttga aattgtactt aacataatat
     1561 tcttaacgat agaatatctt atttcttttc ccatcttata gtaagaatta agcgattctt
     1621 tataatattc ttgaataata tctcgttgac cgatacctct attggttaca actcccttta
     1681 attgctgtag gctatccact tcttctatcc acgatttgtc aa
//
LOCUS       DATRNX010000172         1546 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRNX010000172 DATRNX010000000
VERSION     DATRNX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            384..731
                     /gene="rplS"
                     /locus_tag="VBC81_001958"
     CDS             384..731
                     /gene="rplS"
                     /locus_tag="VBC81_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6912957.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            842..913
                     /locus_tag="VBC81_001959"
     tRNA            842..913
                     /locus_tag="VBC81_001959"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:875..877,aa:Arg,seq:cct)
     gene            complement(876..1490)
                     /locus_tag="VBC81_001960"
     CDS             complement(876..1490)
                     /locus_tag="VBC81_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912958.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
BASE COUNT          496 a          234 c          291 g          525 t
ORIGIN      
        1 cttaggttag gataatgtct acaagcattt aagtcgcgat ggtgaaatgc attagaagat
       61 aaaaagttga tgtggatttc aactttatgg gaaagagtca ggtgacacga aagctaatat
      121 agaaaacgta gtttaaaaaa tcatataata ttaaatgaat taaactctca aaaatcagaa
      181 aaaagctaac ctagaaacag taaatcaaat ttcaataata tacaaataac tattctaaag
      241 gatgacacct tgttcttcta gataaaatag ctttacaagc aggttaaaat ttggtagaat
      301 tgtgagagtg tgtaatggac gcacaaaaaa cggctaatcc gctgagacaa gtacttaaga
      361 ttagtaagag aaggagaata aaaatgaatc cattaattca aagtttgaca gaaggtcaac
      421 ttcgttctga tatccctgag ttccgtgctg gtgatactgt acgtgttcac gctaaagttg
      481 ttgaaggtac tcgcgaacgt attcagatct ttgaaggtgt tgttatctca cgtaaaggtc
      541 aaggaatctc agaaatgtac acagtacgta aaatttctgg tggtatcggt gtagagcgta
      601 cattcccaat tcacactcct cgtgttgata aaatcgaagt tgttcgttat ggtaaagtac
      661 gtcgtgctaa actttactac ttacgcgcat tgcaaggtaa agctgcacgt attaaagaaa
      721 tccgtcgtta attttgatga tcagatttta aaaatgcttg gttgtttgag gatagtaact
      781 atgttttaaa actggacaac caagacgtaa aaaatctgcc tgtgggcagt ttttttacta
      841 ggtcccctta gttcaatgga tataacaact ccctcctaag gagtaattgc tggttcgatt
      901 ccggcagggg acatattcat tgcatgtaaa tagcggttta gagctatttt gccccaaatc
      961 cgccccaaat ttctctgatt aagtttatcg ttcctatctt tttgttcttg taattgatgt
     1021 gcgtaaactt ctaaagtgat atttaaattc tcgtgatcta aaacttgaga gatggaaatt
     1081 agatagcttg caaatgtatg cctgagagag tgcactcgta cctcgcgacc agttattttt
     1141 cggatagttt tattgactgc attatttgaa agtttgtcga ataatctgtc gtttttattt
     1201 tttgtaaatt catgcaaaaa aaataatgta tcattgtcaa ttggtatatt tctgatacta
     1261 cttttgtttt ttgttggcag gtatctttgg ttgaaatgat aatcccaagt tttattaatt
     1321 gataaatatt tgttagtgta atcaatatca ttaactgtta aacctaaaca ttcagcgaag
     1381 cgcatgccag ttttagcgat gaggtataac gctgcatacg attgatgttg tgatttttct
     1441 ttacaaattt ttatcaagcg taagtattca ttggtttcaa gaaattttat ctttatttac
     1501 gccccttatt ttttgcttta accttagtga ataaacaaaa attttt
//
LOCUS       DATRNX010000173         1535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.176, whole genome shotgun sequence.
ACCESSION   DATRNX010000173 DATRNX010000000
VERSION     DATRNX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            172..581
                     /locus_tag="VBC81_001961"
                     /pseudo
     CDS             172..581
                     /locus_tag="VBC81_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            656..1336
                     /locus_tag="VBC81_001962"
     CDS             656..1336
                     /locus_tag="VBC81_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6912959.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
BASE COUNT          585 a          173 c          282 g          495 t
ORIGIN      
        1 gataattaag gaaggaatat tgattagaaa tgaataattt ggatacacta tattgtaatt
       61 tagcaatgtg caatcatagt actaatggaa aggtagcgac ttcagcttgt tgatttatct
      121 aatgtaaatg agtttttaga attttcatct gattttgaaa ctgtatttca tatgcaacga
      181 tataaagcca ataccacaga agaagcacat tgattttggc gaatgtctgt atgaaatcat
      241 tattaggaat ctatgcgatg attgaaacag aatcaaataa aatgattggt attatagaat
      301 tagaaattag agacgagttt ccagcagaat tcggttatat attaaataaa aattatagtg
      361 gaaaaggtta tatgataaaa gcttgttcaa aattgatgtc aattggattt gaacaactag
      421 atttggaaag aatttatgct cgatttgaac taataataaa aaatctgaaa atgttatgga
      481 atgcctaaga atgcaaaaag aaggtgaacg tcgtcatatt gtaaaaaaat ccaaaaggtg
      541 agtggaaaac cagagcatat tattctattt taaaggaata atattttaaa tagacgacaa
      601 taaaaatttg tgaaagcaga aaaattaaat gagatacaat aggtttaatc aactagtggg
      661 tgatgtcgtt gaaaatttta cggaaggtaa aaatccgaag attgatacac ttaatgggaa
      721 aacagttaga atagaaaaaa taaatccaga tcattttgaa gacttatttc aagtttatgg
      781 agaattgtct actgaagata gtttaactta tatgtctttt agcaaattta atagtaagaa
      841 tgaatttgat gtctttttcc aaactctatt aaaatcagaa gatccttatt atttagctat
      901 tgttgataat aatactggta aagcattagg gactttttca ttaatgcgta ttgatacaaa
      961 aaatagagtt gttgaaatgg gatgggtggt ttattcatca aaactaaaac aaacaaggat
     1021 agcaacagaa gctcaatatt tggttatgaa atatgttttt gaagaactct gttaccgccg
     1081 ttatgagtgg aaatgtgata gtttgaatgc accatctaat aattctgcaa aacgacttgg
     1141 ttttactttt gaaggaactt ttagacaagc tgttgtatat aagggtcgta acagagatac
     1201 aaattggtat tctattttag ataaagaatg gccagagaaa aaaacacggt ttgaaagatg
     1261 gctagatgat tcaaactttg atgtaaatga gtatcaaatc agatcactat caagcattga
     1321 gcagaagaca aaatagagtc attcattaat cactaaaatt tcaaaattac atgttttttt
     1381 agctaacatc ttaaactttg ttaaataaat gtttattaac ttttaaaggt gcatggtagg
     1441 actcaaaatc gggtgtcctc acggacgtgc tagctcgact ttggtcgcgg gcatcaaaga
     1501 aaaaagtcaa gattattatc ttgtcttttt ttcta
//
LOCUS       DATRNX010000174         1510 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRNX010000174 DATRNX010000000
VERSION     DATRNX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..528)
                     /locus_tag="VBC81_001963"
     CDS             complement(<1..528)
                     /locus_tag="VBC81_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6912960.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQA"
     gene            complement(530..1255)
                     /locus_tag="VBC81_001964"
     CDS             complement(530..1255)
                     /locus_tag="VBC81_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912961.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            1327..1510
                     /locus_tag="VBC81_001965"
                     /pseudo
     CDS             1327..1510
                     /locus_tag="VBC81_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          509 a          290 c          193 g          518 t
ORIGIN      
        1 agcctgtttt ctttcctgct catgatttat agctgcatcc cagttcaaat tttgattgtt
       61 ataaaagacc tttaccttgt aagttatcac caaccatttg attattccta gaccaaaaat
      121 taacaatgta atcataaaaa ctgacaagcc taactttatc aaaattggtg atattaatac
      181 taaaaataag gtttgaacaa ccaaccaaaa gatataagtc cgttttttag cagaatttat
      241 aatttccttt attgcttctt cttttataag taaaaattgt ttatcagccg gttctaaata
      301 gctggctatt tcccccatcg ctagtaacat taagataatg attgacacaa tcacgataat
      361 tggccaatgt gtttttggaa agtcttttag taactggcta tactgatata acaaaaaacc
      421 tgacaaaaac attaatacta atacaaaatg atcattaaag acgtatctca aatatttact
      481 attttgagtt aaaaagagac ttcgacgttt attaaataac tttttcatat cacacctctt
      541 ttgtcagagc tatgtagatg tcatttaaat tagcattact gtctccaaaa atagcccgta
      601 attcctctag ggttccaact gcccgtattt ctcctttatg taaaataaca aaacgatcac
      661 acattttttc ggcagaatct aatacgtgtg ttgacatcaa aatagatttc cctttagctt
      721 tttcttctgc tagaagatta atcaaatctg aaatagctag tggatccaaa cctaaaaatg
      781 gttcatcaac aataaataaa cttggagata caacaaaagc acagataatc ataacctttt
      841 gtttcattcc ctttgaaaaa tgcattggaa accagtctaa tttatctgtt agacgaaaca
      901 tctctaatag tttttgcgca cgtgccataa cttcatcggt ggcaatatca tacgccatag
      961 ctaccgtttc taaatgttct ctgagagtta gttcctcata gagacttggt gtctcaggaa
     1021 taaagcctat tttctttcta taaagttctt ggttggcttc aagactaatg ccatcaatgg
     1081 taatatctcc ttgatagggt ctcaaaatac caataatctc gttaatagtg gttgattttc
     1141 ctgcaccatt aagcccaatt aatccaacta actctccatc atttacttca aaagaaatat
     1201 tttttaaaac tgggatatta acataccccc ctgtaacatt ttcaattttt aacattgtca
     1261 ttctctctat ctttgatata ctttattaac tattataaca aaataagaaa gggaaggtct
     1321 attcttatgg ataattgtat cttagaattc ccttatagtt ttgaaaataa ctttaaacct
     1381 attttgtccg cttttacatc taattgaaat gccaagctac cccatgctcc cattatgttt
     1441 acaaaaagca cctccatcac tcatcggaca aaaaaagaca cctcaaaaag gtgtcttttt
     1501 agtatcaata
//
LOCUS       DATRNX010000175         1469 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.178, whole genome shotgun sequence.
ACCESSION   DATRNX010000175 DATRNX010000000
VERSION     DATRNX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..926)
                     /locus_tag="VBC81_001966"
     CDS             complement(<1..926)
                     /locus_tag="VBC81_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912962.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFLFLSAVPGYLIYIGESYSKF"
     gene            complement(1151..>1469)
                     /locus_tag="VBC81_001967"
     CDS             complement(1151..>1469)
                     /locus_tag="VBC81_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912963.1"
                     /translation="GYSDGMKSKLSLICLMISKPKVILLDEPLTAVDVVSSIAIKRLL
                     LELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQFEERLLQVLKGDE
                     YDK"
BASE COUNT          539 a          262 c          221 g          447 t
ORIGIN      
        1 caaaattttg aataggattc accgatatat attagatatc ctggaactgc actcaaaaat
       61 aggaattttg aaaaactatg ctcattcatc ggaatattta tcttttcttt aaaaaatagc
      121 aggaaaagga gttctattcc aaacagaaca cttaaaacag ctaaacgtac caaaattttc
      181 ctttgcaagt atctactcat ccgttttaaa aagatagcgt ttaaatatga aatacctgat
      241 aaattttcaa atagcagggg agaaagttca ctgacattta tatccctatc tttcatttca
      301 atcccaagca gctctatatt atcaatcttt gcaattttct ccttcatcgt ctcatgcgtt
      361 agggtaaggt aggtcaagtg gttaagttta gaataagttt ttaatcgata gtaactaact
      421 aaccatatga taagtcctgc aataccggtg taccatgaaa aaaatggata tggagagaac
      481 tgtttgccaa gaagaaggaa aaagagagaa aggaagactc ctataccaat ccctaagtaa
      541 aataaaatat ttagtaaaga ctctggattc tcttggtctt tacctctacc atataaaggt
      601 aaacagagca ccttgattcc aaggcgacta cctgctaaaa ataaagcaaa agtcaaacca
      661 tgccaaaaag caaagtgatt taaatggaaa aatatgccaa agctcaaact tctaaaaaat
      721 agttcactac tggcttctaa aagttcagcg gattggaaat acctcgatgg ctcaattctg
      781 aaaaggcgaa tcatatgagt ttgttgcata tcattaggtt ctataagttt tatatagaaa
      841 tttatgacaa ttaaaagaga ggtaatcacc atcaatgtta aagttagggt aaaaccttct
      901 tgcggtaaaa tatttaactg cttcatgcca cttctaacaa aaaatgataa aacataacaa
      961 tatagtacgt aacctatgga acgaccaaaa cctcttaaga aaaagaaaat gatacgaata
     1021 aatagtacta caccttttat accatcggtt agggattttt cttcccaacc aggggatttt
     1081 ctgaaggtaa tataaaaaac tattataagc tctagcacta tcaatctgta atatagacca
     1141 tattaatgct ttacttgtca tattcatctc ccttcaacac ttgaagaaga cgctcttcga
     1201 attgttcatg tttacgatca atatctaatg tttggagttt tcctttgtct aatacagcca
     1261 caatatcaca tagatcttct gctaaggcca ttatatgagt tgataatata ataatatgat
     1321 cctcacttaa ttccaacaaa aggcgtttta ttgcaatact tgatacaaca tcaactgcag
     1381 tcagtggctc atctagtaaa attacttttg gcttagaaat catcaggcaa attaatgaga
     1441 gcttactctt cattccatca gaatatcct
//
LOCUS       DATRNX010000176         1456 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.179, whole genome shotgun sequence.
ACCESSION   DATRNX010000176 DATRNX010000000
VERSION     DATRNX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(4..357)
                     /locus_tag="VBC81_001968"
     CDS             complement(4..357)
                     /locus_tag="VBC81_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO6912964.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPYYLILISLLAIGITIKLLRTKTLFSYKRFLKLFWRSGFILTF
                     LFYLGLLVIIFIKVQ"
     gene            complement(418..1323)
                     /locus_tag="VBC81_001969"
     CDS             complement(418..1323)
                     /locus_tag="VBC81_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6912965.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          502 a          194 c          263 g          497 t
ORIGIN      
        1 tttttattgt actttgataa agatgataac caataatcct aggtaaaaaa ggaaagttag
       61 aataaagcca gatctccaaa ataattttaa aaaacgttta taagaaaaaa gagtctttgt
      121 tcgtagaagc tttattgtaa ttccgattgc cagtaatgaa atgagaatca gatagtaagg
      181 taaaagattt tgttttaaca gactagatga tattaatgga attaaaaaaa caaaaatagg
      241 aaaagcaaca tctggaaaca taatgttaaa ttttttaagt ttaaatattt tgacaagaac
      301 ctgtgaaaat atagggatta aaacaagaaa taaagaagca ataattttgt atatcatagg
      361 tctattttat accgattttg gtaagaagta aactgatttt caatagaatt agcgaaacta
      421 cttggttaaa aaagctttat aaagtaggag agcaattcca gataattcaa catctttttt
      481 ataaatcata atgtactctg aaaataactt attcttatga aaatggtaga cattgcactc
      541 tttatccaaa tagcgatgaa tgagagtttg tggaacaaat gttgctccca tccctgacaa
      601 agataggcta gctgctgtag gaacggtact agttgtaatc atctgattta atgacatgtg
      661 atgaagctca tagaattgtg caattgtttt gccaattccg tactctaatg gtgttaggat
      721 aagaggttca ttgtctagaa ctgaaatagg atattcaaaa ggaataatct gtccacgttt
      781 ttcagcgttg tagagttggc tggtggtagg tatcattaaa aacaattcgt cgcggtaaat
      841 agtggttgat gcaattccat ctttataaag gataggagcc atcccaacag ctaggtcaat
      901 atcaccactc gctaccaatt gttcagaaat attttgattg ttttcgagta atttgacaga
      961 aatttctggg tgctccaagt taaatttggg aagtacattt gccagaatag aacttgctaa
     1021 gccagagtta atcccaagtg tgatttctcc ttttttatca gaacggatga gatatagttc
     1081 tttggccatt tgacgctcaa tcatatcaat ttcttttagg tagaatagat atctctgtcc
     1141 agcataagtt aaaaaggttt ggtgtccttg gctctggatg agttttattt ctaactcatt
     1201 ttctaattgc ttgatcactt tgcttaggta aggttgagaa ataaaaaggt gtgcagcggc
     1261 tttagtgatg tttgagtata gctcgatact ctctagatac attaattttt gttgattttt
     1321 caagacttta taaccagttt gatatcctta gattaataat aagttatgat acgttatggc
     1381 tataagttta ctatcatttt gagctaaaat caatctattt aatttagtag tataacatga
     1441 tatattcata actatt
//
LOCUS       DATRNX010000177         1434 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.180, whole genome shotgun sequence.
ACCESSION   DATRNX010000177 DATRNX010000000
VERSION     DATRNX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(1..1341)
                     /locus_tag="VBC81_001970"
     CDS             complement(1..1341)
                     /locus_tag="VBC81_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6912966.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
BASE COUNT          535 a          272 c          228 g          399 t
ORIGIN      
        1 ttatattgct tttctttttc gaagataagt taatcctaca atagctaaga taacaacaac
       61 gataacgaat agaactgcaa attgactacc aattatagct ccatttttcg gaatagctac
      121 ttgtccgatg gtaaaaattt gtgaggctac tgcggttgca aaagctcccg caaattgttg
      181 tagtgtattt aagatactat taccatccgc tgactgtgct ggctttagtt ttgttaaagc
      241 tgtcgtaacc atattacttg ctccaatccc cataccaatt ttagtaagaa tatcaaaggc
      301 taaaagtact acaacaaact tcgcaggaat gaatatagct gtaccaatta aacctatcaa
      361 agaaagggag atacctatca taattggctt aaatgcacca attttatcca ggagataacc
      421 tgcaaaaggg gataaaaaaa ctactgctat tgcacctgga aacataaaca aaccagcttt
      481 ggttgaagtt tgatgtaaaa caagttgcaa gaggttaggt aataagaacg aaagtgctag
      541 aggaatcatt tgaaaagcca ggacgccata caataagatt gagaaatctt tatctttaaa
      601 aacattcaaa ttcaaaaatt ctagctgttt atttaaatag taaaaaataa caaaactaac
      661 aatagctact aataagtaaa aaatagacat cttttcaata gctaaaagaa gggttgccaa
      721 gccaatacct aaactgacaa aggcaggaaa attgagttta tcattaattc ttacttgacg
      781 tttgggaata gaagccaaac ctataaaggt agatatgata agtattggtg ccagaagcat
      841 aaaaatcatc ttccatccta acattcctga aatgacacca ccataagtag gtccaactgc
      901 cggtgctaaa agtgtgacca tagcagctac tcccatcatt agtcctctac gctccatcgg
      961 aatttgtgta agaatgatat gaaacaataa agggagggct aatcctgtcc caacaccttg
     1021 caagactctc cctactaaca atatagctaa attaggggca aagctatcaa ttaaaacacc
     1081 aaataaaaaa agcacatttg ccaatgtaaa aatttgtctc tctgataaat ttttaatcat
     1141 aaatgctgac aaaggcacag tcacagcaac agctaacaaa tttcctgttg tcacccattg
     1201 aataacagcg gggttaaccc caaattcttt catcaaaagc ggaaaagtga cattcataga
     1261 tgtttcaata agaatccctg aaaaacctaa tatagccgtt gtaattatgg caggcataat
     1321 acgccatccc tttttagtca ttaaaatctc cttactttga ttattatcat aaaatagagc
     1381 accccgctaa atcaaggtgc tccatttaat tcattcgaat taaaattaaa ttgt
//
LOCUS       DATRNX010000178         1419 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRNX010000178 DATRNX010000000
VERSION     DATRNX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(239..550)
                     /locus_tag="VBC81_001971"
     CDS             complement(239..550)
                     /locus_tag="VBC81_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6912967.1"
                     /translation="MSNDSIFSGIYSPNSSSQVPTTPQYQAPSPSPQAPTTPQYQAPS
                     PSPQAPTTPQYQAPSPSPQAPTTPQHQAPSPSSQIPSTPNTPSNNFGSGDNGIDFGEG
                     L"
     gene            522..>1419
                     /locus_tag="VBC81_001972"
     CDS             522..>1419
                     /locus_tag="VBC81_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6912968.1"
                     /translation="MPENIESLDIQIWVNKLAKQSVKSVASGVKKSKKGHSKDFGAFV
                     HKLDDIFDFGVINFGLQSNPVKGIKIPPKPKSLKKRIRVLHDNELTIWLEYLEILENN
                     RANRRFKIICNTLLASALRINELLALTIDDLDFMTNEIIVSKTLVWKAGNKNKGIKGE
                     MICKQSPKSDSGNRRVLVPKEILNGLKDFHQEMNNYFEDHRLPTTKLIFPTIYGNYMC
                     DRNERLTLKKRLSSLNLPDYGFHIFRHTHASMMLNAGANWKELQVRMGHKSISTTMDT
                     YAELAPTKKQEAVEIFLNKISEL"
BASE COUNT          498 a          196 c          262 g          463 t
ORIGIN      
        1 aatattgtgt acacacttca aaacttagag agtctttttc actaagaaat tcttgatatg
       61 ttatcattta atttcaccta aaaccactta ataaaagtgg aagaatactt cccataagaa
      121 ttatttatat ttgataatat atcatatttt ataatattat ataactatta attaatcaaa
      181 aagaaccttc aaaggttctt tttatttttc ttcaaaaatt gatataaatt ctattttttt
      241 ataatccctc cccaaaatca attccattgt ctccacttcc aaagttatta gaaggcgtat
      301 taggtgtaga cggtatttgt gagcttggag aaggtgcctg atgttgaggt gttgtcggtg
      361 cttgtggact tggtgaaggt gcctgatatt gaggtgttgt cggtgcttgt ggacttggtg
      421 aaggtgcctg atattgaggt gttgtcggtg cttgtggact tggtgaaggt gcctgatatt
      481 gaggtgttgt cggtacttga gagctagaat tcggagaata tatgcctgaa aatatcgagt
      541 cattagacat ccaaatatgg gttaataaat tagctaaaca gtctgttaaa tcagttgcat
      601 caggtgttaa aaagtcaaaa aaaggacatt ccaaggattt tggagctttc gtccataaac
      661 tcgatgatat tttcgatttt ggagtaataa attttggatt gcaaagcaac cctgtaaagg
      721 gaattaaaat tccgccaaaa ccaaaatcat taaaaaaaag aattagggta ttacatgaca
      781 atgaactaac tatttggtta gaatatttag aaattttaga aaataatcgg gctaatagaa
      841 gatttaaaat aatatgtaat actcttctag catcagcact tagaattaac gagctattag
      901 ctttaacaat tgatgattta gattttatga caaatgaaat tatagtcagc aaaactttag
      961 tgtggaaagc tggtaataag aacaaaggta ttaagggtga gatgatttgt aaacaaagtc
     1021 caaagtcaga ctcgggaaat cgtcgagttc tagttccaaa ggaaattttg aatggattaa
     1081 aagactttca tcaagaaatg aataattatt ttgaagacca taggttacca actacaaaat
     1141 taatttttcc aacaatctat ggaaattata tgtgtgatag aaatgaacgt cttacattaa
     1201 agaaaagatt gtcatcactt aatttacctg attatggctt tcatattttt cgtcatactc
     1261 atgcttccat gatgctaaat gcaggcgcaa actggaaaga gcttcaagtt agaatggggc
     1321 ataaatccat ttctaccact atggatacgt acgcagaatt agcacctaca aaaaaacaag
     1381 aagccgtaga aatttttctg aataaaattt ctgaattaa
//
LOCUS       DATRNX010000179         1412 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.182, whole genome shotgun sequence.
ACCESSION   DATRNX010000179 DATRNX010000000
VERSION     DATRNX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.182"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..324
                     /locus_tag="VBC81_001973"
     CDS             <1..324
                     /locus_tag="VBC81_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912969.1"
                     /translation="LSSVSGNYNNFTVENINQTFEKGDKSLKDIIEIFGKPDTIWVNY
                     VDNQYNTDLYWKKDDTDDSNYIHAEWSEPKDEYDVQSLSGTASNSGTGKFQSFIGLDG
                     FEPAQ"
     gene            432..>1412
                     /locus_tag="VBC81_001974"
     CDS             432..>1412
                     /locus_tag="VBC81_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HEO6912970.1"
                     /translation="MEVKKFSEKDFVNEINKIKQKQFLSQIEQYESYIAPQMRTKGYK
                     RINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLAT
                     MMPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLY
                     VEGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELRDKHEILQLNYDKAKYN
                     LLDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLS
                     SYLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKK"
BASE COUNT          553 a          164 c          253 g          442 t
ORIGIN      
        1 ttatcatcag tatcaggtaa ttataataat tttactgttg aaaacatcaa tcaaactttt
       61 gaaaaaggtg ataaatcttt aaaagatatt attgaaattt ttggaaagcc tgatacaatt
      121 tgggttaatt acgttgacaa tcagtacaat actgatttgt actggaaaaa agatgacaca
      181 gatgattcta attatattca tgcagaatgg tctgagccaa aagatgaata tgatgttcaa
      241 agtttaagtg gcacggcttc aaattcaggt actggcaagt ttcagtcatt cattggatta
      301 gatggatttg agcctgcaca gtaggataaa tagaaagatc acgttattaa atgtggtctt
      361 ttactttaac ttttcgaata aaaaatagca ataatattct aatcctgtaa caaaaattgt
      421 aaaatattat catggaagtt aaaaaattct cggaaaaaga ttttgtaaat gaaataaata
      481 aaataaaaca gaaacaattt ttaagtcaaa ttgaacagta tgaaagctat atcgctcctc
      541 aaatgagaac gaaaggctat aagaggatca atcagtctga acgtacggtt gttttctctt
      601 ttggagaaat aacatttagt aggagtcgct ggacaaatgg ctttgaaact agaataccag
      661 tagatgagtg gttaggtctt gaaaaatata agagatattc aatagaattc ttatatcatg
      721 ttgcaaaatt ggctacaatg atgccttatc gtcaagtttg caaagtaata gatagcactt
      781 tgcaaacaat cataacaaaa gactgtgttt taaaagcagt aaaatttgta gaaaaattgt
      841 taaaagaaaa agaacgctat cgtttttatt tggaagagcc acccgaacgt aaaaaagtga
      901 aaaaactgta tgttgagggt gatggagtca tgattaaaag cacagattct agagaggaaa
      961 gaaggtattt agatttaaca cattttgtta ttcatacagg ctcaaaaaaa gtttctacta
     1021 aaagatatga attgcgggac aagcacgaaa tattacagct taattatgat aaagctaaat
     1081 ataatctttt agattatatt tataataact atgaagtaga tgacgatact attttaatca
     1141 ctaactctga tatgggtaaa ggctatacta gtagagtttt taaggaatta ggaaaagcac
     1201 ttaaggtaaa gaaacatgag catttttggg atatctatca tgttaaagaa aagttaagtt
     1261 cataccttag aaaatatcca attgaattaa ccgattttgc tttagatgcg gtaaaaaaat
     1321 ataattctga taagcttgaa ttagtttttg atactgttga atcactgatt tgtgatgaac
     1381 ttgaagatca agaatttcag aagtttaaga aa
//
LOCUS       DATRNX010000180         1411 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.183, whole genome shotgun sequence.
ACCESSION   DATRNX010000180 DATRNX010000000
VERSION     DATRNX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(100..666)
                     /locus_tag="VBC81_001975"
     CDS             complement(100..666)
                     /locus_tag="VBC81_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6912971.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(659..>1411)
                     /locus_tag="VBC81_001976"
     CDS             complement(659..>1411)
                     /locus_tag="VBC81_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6912972.1"
                     /translation="IREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKERYE
                     QAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALINRAH
                     SYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKLHLL
                     HLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLIGPM
                     WSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
BASE COUNT          408 a          272 c          222 g          509 t
ORIGIN      
        1 tttttatcag actaaattcc atttctacta gaggtggaac ttactttgag aatttagctt
       61 ttatagcaat tatcattttt gctaattgcc taaagatagt tacacctcat tttcatatga
      121 ttgtaacttt tctaccataa ttaaaaaggg gggcgtatta acttgattga gtggttgata
      181 aagcattgta gcgaaattat tctgtggtaa ttgttccacg aatgctatca ccgcatctct
      241 ctcacttttt ccaccatcat gaccataata aaccataagt gatagacgtc cacctacttc
      301 taaaatgtct aaaacgtttt tgattgcctt gatagttgtg tgtggttttg taataactgt
      361 tttatccgct gagggtaagt atcccagatt aaatatagcc gctcttaaag gtgtatgaac
      421 atactgttcc aaattttcat gcccatccaa gataagttca gcgttagaga tcccttgctc
      481 cgtgagcttt gctttcgttt ttgctattgc ttcttcttga atatcaaatg cataaacttt
      541 tttagaactt ttcgctaaaa aaacagtatc attcccattt cccatagttg catcaagcgt
      601 tattgcttct ttatcaataa cttctgctaa aaaatcatga gaaagatgaa ttggtctttt
      661 aatcatgttt tacctcaact ttacatcctt gatatgactg tcttttctcc atttctttgt
      721 caatggcatt aagcacttcc catttattaa ggctccacat cggtccaatt aacatatgac
      781 gtggcgcatc accagtaatt cggtgaataa caatatgttt tggaataatc tccagttggt
      841 cacaaataat cgaaatatag tcttcttgac ttaacaaacg taaacgacct tcatggtaat
      901 ctctctgcat tcttgtatta atcataagat gcagaaggtg taattttata ccctgaatat
      961 cattatccgt aacacatcga cgaacatttt ccaccatcat atcatgtgtc tcccctggga
     1021 gaccatttat taggtgagaa acgatttcta ctttaggagc taattctcgt actctcttaa
     1081 cagttttttt gtaaaggtca tatgaatgtg ctctatttat taatgcagaa gttgcttcat
     1141 aagttgtctg aaggcctaat tctaaagtca catgcattct ttcagaaagt tcagcgagat
     1201 agtatatagt ttcatcaggt aagcaatccg gccttgttcc gatgttgatc ccgataactc
     1261 ctggctcatt aatagcctgt tcgtaacgct ctttaattat ctctaactta gcatgggtat
     1321 tggtaaaatt ttgaaaatag actaagtatt tattaacctc aggccacttt ctatgcatga
     1381 aatcaatttc tttatagaat tgttcacgaa t
//
LOCUS       DATRNX010000181         1407 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.184, whole genome shotgun sequence.
ACCESSION   DATRNX010000181 DATRNX010000000
VERSION     DATRNX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            141..>1407
                     /gene="srr2"
                     /locus_tag="VBC81_001977"
     CDS             141..>1407
                     /gene="srr2"
                     /locus_tag="VBC81_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO6912973.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYN"
BASE COUNT          505 a          216 c          274 g          412 t
ORIGIN      
        1 tatgctattt attgatgatg tttcaagcaa tattttagaa cttgaaataa caaagtagta
       61 ttttgttata gctatatttg agaattctta gaattagaaa agttttttat atagcattat
      121 tttttaaata tggaggaaaa atgttaaaaa agcagtttgg aaactttggt gaaaaatcac
      181 gcaaagttcg agttaaaatg aggaaatcag gtaagcactg ggtcaaaagt gttatgacac
      241 aaattggcta tgttatcctt tcgaggttta gcggtaaaga gaaaagctct aaggttcaaa
      301 ctactagtga agatttaagc agaactaaaa cgtctgctag catcctaaca gcagtcgcag
      361 ctcttggagc tgtagttgga gggacgacag acacaacttc tgtctcagct gaagaaacac
      421 ctactgcaac agaacttaca ggaaatgaaa aaacattagc tactgctgaa actgtagtgg
      481 ttgcgccaga agtaaaaact gtaaactcag attcttcatc acattcaaca agtgaatcac
      541 aatcaatgtc aactagtacc ttgcaatcta ccagcgcctc attatctgcc agtgaatcac
      601 taatggattc tacctctgct tctttatctg agtctagctc gttatcagaa tatagttctt
      661 tatctctatc tagtagtgag agtgtctcag catcagaatc agttcaatca tcagaagcgg
      721 caacgaccgc tagagttcaa ccgagagcga tgagagttgt atctagtgct tcagatatgg
      781 aaactttacc agcagcatta atctctggtg aaggagatgt aacaactgtt caaggacaag
      841 atgtaacaga taagttacaa aatttagata taaagctctc tggaggagtg caagcaaaag
      901 caggtgttat aaatatggat aagtcagaaa gtatgcacat gtctttaaaa ttcactattg
      961 attctgtgaa tagaggtgat acttttgaaa ttaagttatc agataatatc gatacaaatg
     1021 gagcttctaa ctattctatt gtagaaccta taaaatcacc tacgggtgag gtctatgcga
     1081 ctggaattta tgattctcaa aaaaaatcta tagtctatag ttttacagat tttgcggctt
     1141 caaaaaataa tattaatgga atattagata ttccattatg gccagatgat acgacagttc
     1201 aaaacacaaa agaagatgtt cttttttcgg taaaaataaa ggatcaagag gctacaatta
     1261 aagaaacagt gaaatatgat ccaccggtaa gaattgattt tgcaggggga gtaagtgtag
     1321 attctcgaat aaccaacatt gatgatgtgg gaaaaaagat gacttatata agtcaaatta
     1381 atgtagatgg aaaatcactc tataatt
//
LOCUS       DATRNX010000182         1339 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.185, whole genome shotgun sequence.
ACCESSION   DATRNX010000182 DATRNX010000000
VERSION     DATRNX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.185"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(31..1251)
                     /locus_tag="VBC81_001978"
     CDS             complement(31..1251)
                     /locus_tag="VBC81_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6912974.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
BASE COUNT          520 a          264 c          190 g          365 t
ORIGIN      
        1 aaaaccaaag aattttaaat tagatatata ctacacaact ttagttgtta attttcttaa
       61 atatagagat aacaataatc cgaataagaa taaaaaacca aaaaccatta atgtcaattg
      121 ataggaattt ccccatgaat atgtctttga taacaatagg ggcccaaaca gacctgctat
      181 tgcccatgct gttaaactat aaccatgtaa agtagctaat tcctttgttc caaaaatatc
      241 acttagataa gcaggtaata aggaaaaacc tgcaccataa caagtcatta aaatagacat
      301 cgctattgca aatacaatag cattgaatga caaaaataaa ctagaagtca taataaagtt
      361 cacaataaat aatattataa aggtcaacgg gcgtccaatg tagtcagaga gacttgccca
      421 taacagacgt ccaaaaccgt taaatatccc tagtacccct actaataagg ctgcagattc
      481 tgcggaatag cctgctaaat cttgtgccat tggtgaagct gctgatatta aacctaagcc
      541 acacgaaata ttaataaaca aggtcaacca aatgatgtaa aaggtttttg attttatagc
      601 gacgtttgct tttaatccag tgataatttg tgaattcata gcattctttt taccatcgtg
      661 agtcaaaata gttatttttt cctgaggtgg ttgtttaata aattgtgagg caatcatcat
      721 gacaaaaaaa tatactaatc ccaaaatata aaacgtttta cccacaccaa tcctaatcag
      781 taaggattgt gcaagcggac ttgttactaa agaagcaaag ccaaatccca taatagcgaa
      841 tcctgttgct agtcccctcc tatcaggaaa ccatttaata atagtcgata ctggagtaat
      901 ataacctgaa ccaagtccga ttcctcctaa aataccgtat gcaacataca gtaaccataa
      961 ctgctgagtt tcaatggcta agcctgttaa cacattccct gctccatata aaatagcaga
     1021 aatcattccc attatcctag gaccaaaacg ctctactaag tgtcccataa aagctgcaga
     1081 cattcctaga caaaaaatag ccaaactaaa agcgaatgaa actgatgaaa tatcccaacc
     1141 agtctctgag ataattgggt tacgaaacac actccaagca taagttgatc ctagcattaa
     1201 atgtaaaacg actccagaaa ccgcaactac ataacgattt aaatttttca aataatcctc
     1261 ctaaaaacga acttaagttt tatttttaaa aatatagttc gcaataaaca gattatcaaa
     1321 aaactgaata tatcaccag
//
LOCUS       DATRNX010000183         1293 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.186, whole genome shotgun sequence.
ACCESSION   DATRNX010000183 DATRNX010000000
VERSION     DATRNX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.186"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(1..532)
                     /locus_tag="VBC81_001979"
                     /pseudo
     CDS             complement(1..532)
                     /locus_tag="VBC81_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            710..910
                     /locus_tag="VBC81_001980"
     CDS             710..910
                     /locus_tag="VBC81_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6912975.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            952..1140
                     /locus_tag="VBC81_001981"
     CDS             952..1140
                     /locus_tag="VBC81_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6912976.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          450 a          171 c          251 g          421 t
ORIGIN      
        1 gaacctgttt gatattttga taattttttt ggttgatttg tctatgcttt aaatacttga
       61 aaaatgcttc agttacggca ttatcataag gatatctagg tttagaaaaa gaatgcatga
      121 tattgttgtc atcaattatt tttctaaatt ccctggcctt aaattgtgac ccttggtcgg
      181 aatgaaagag aagtgttcct tcaatctttc ttttattaag agctaattct agagtgtcac
      241 atgctaactt tgcatccata cgatgactca atttccaagc aatatatttt ctagaataga
      301 gatcaattat tgcgcagaga tagacatatt tcttatatcc aatagaaata taggtgaagt
      361 cagtagacca tacttgatta ggtttatcag gattaaattt ctggttgagt aagttttgag
      421 gatacgtttt ttgagtcttt ttaagagctg tttttgtctt aacggtagcc attttaggga
      481 gcgccatatt cttcatcaga cggtaaattc ttccttcaga gatttttgtg tcttttaaaa
      541 caggttataa cacacaaagt gttgtctatt tttatagtat acatccatac tcgcataacc
      601 atgagataat cactaaaaca agagtcgcct gacactagcg actcttgttt ttatttgcta
      661 aagaaagata tagatgttat gattaataaa aataataagg aggtgcagta tgtcacaaga
      721 aaaactaaaa tcaaaattag atcaagcaaa aggtggtgct aaagaaggct ttggcaaaat
      781 aaccggtgat aaagagttag aagcaaaagg atttatcgaa aaaacaattg ctaaaggcaa
      841 agaactagca gatgatgcta aagatgctgt tgaaggggca gtagatgctg tcaaagaaaa
      901 actgaagtaa caaataaccg ctcatttaac gagcggttat ttgttatctt tttgtctatc
      961 agaacagata tagatgagtt taaagaagcg attgataaag gctatatttc agggaacaca
     1021 gtagcgatag tgcgtaaaaa cggaaagata tttgattatg tgttactaca cgaagaagtg
     1081 agagaagaag aggttgttac agttgagaga gtgcttgatg tactgaggaa gttatcataa
     1141 taacggacca aaattcgccc caaaaagtat tttaaaatag aataaaataa aaaaatatgc
     1201 tttttattat ggatgtatcg tgtattttaa cctttaatat atcctatttt aatggtagag
     1261 aagttggcag gggacatatt aaaacctttt ttg
//
LOCUS       DATRNX010000184         1289 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.187, whole genome shotgun sequence.
ACCESSION   DATRNX010000184 DATRNX010000000
VERSION     DATRNX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.187"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBC81_001982"
     CDS             212..1288
                     /locus_tag="VBC81_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6912977.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
BASE COUNT          458 a          180 c          217 g          434 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaat
//
LOCUS       DATRNX010000185         1286 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.188, whole genome shotgun sequence.
ACCESSION   DATRNX010000185 DATRNX010000000
VERSION     DATRNX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.188"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            261..1184
                     /locus_tag="VBC81_001983"
     CDS             261..1184
                     /locus_tag="VBC81_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6912978.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          428 a          208 c          213 g          437 t
ORIGIN      
        1 agaaataaat taatcacccg tagaggtgta tgtgagtgtt tcttttggtg acggcgcgaa
       61 tgattagaca ttgtaattct ccaatactta aaaaaacctt aattttactt ggatattata
      121 tcatttttca atacaatatt caacgaaaat aatattggaa taatctgatt atattagcta
      181 aataacattt tcaattgatc tatattgata atttagctga tctaattgaa tttatttaca
      241 ttctagttta aactagaatt atgagaattc aacaattaca atatgttatt aagattgtag
      301 aaactggtag catgaatgaa gccgctaaac aactctacat tacccaacct tcactttcaa
      361 acgctgttag gaaccttgag actgagatgg gtatccaaat ttttattcga aatccaaaag
      421 gaataacgtt aacaaaagat ggtatggaat ttctgtcata tgcgcgacaa attctagaac
      481 aaacagcact tcttgaagag cgttataagg gtgataatac aagtcgtgag ctgttcagcg
      541 tttcttcaca acactatgca tttgtggtta acgcttttgt agctctcttt aatggcacag
      601 acatgaccca atacgaactt tttttacgtg aaactaggac ttgggaaatt attgatgatg
      661 ttaaaaattt ccgttcagag attggtgttc tgtttcttaa tagctataat agagatgtat
      721 taaccaaatt attcgacgat aattccttaa ttgccacaac tctttttact actacacctc
      781 atattttcgt gagcaaatcc aatcccttag ctaatagaaa aaaattaagt atgaaagatt
      841 tagaagatta tccttatctg agttatgatc aaggcctgca taattctttc tacttttctg
      901 aggaaatgat gtctcaaatt cctcacccaa aatccattgt tgtcagtgat agagcaactc
      961 tgtttaattt aatgattgga ttagatggtt atacagtggc aactggtatc ctcaactcaa
     1021 aattgaacgg tgacgaaatt gtcgcaatcc ctcttgacgt ggatgatgtt attgatattg
     1081 tctatatccg acacgacaaa gcaaaccttt caaaaatggg acaaaaattt attgactatt
     1141 tattagagga agtttcgttt aatcataaaa ataaacagtc ctgagattgt tgtactgacc
     1201 cccaaaagtt ggacaattta ttttaagtga aggatttagt tctgtattgc acaggactaa
     1261 gtccttttag ttttgctttg attcgc
//
LOCUS       DATRNX010000186         1267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.189, whole genome shotgun sequence.
ACCESSION   DATRNX010000186 DATRNX010000000
VERSION     DATRNX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.189"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..>1267
                     /locus_tag="VBC81_001984"
     CDS             <1..>1267
                     /locus_tag="VBC81_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6912979.1"
                     /translation="VGCQNNKKVKTDKKVDITAYYYHNNTPQYSVQINAIHLQTALDK
                     VRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGIKNTRQDAIAIVRLLLE
                     KNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVGLTAFENSAETQEYVLK
                     NIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRIPIYSKQLTVRVSPNFV
                     VDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLNSSGDETEQKELLQNSL
                     YKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKKGVIETKRSLISVDNQK
                     QDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEYILGRLNADKKVSLYQP
                     NGFWINTQKTIKSLNKEYLNQNAYHIRGN"
BASE COUNT          496 a          158 c          237 g          376 t
ORIGIN      
        1 tagtaggttg ccaaaataat aaaaaagtaa aaacagataa aaaagtagat attacagctt
       61 attattatca taataatacc ccgcaataca gtgttcagat taatgctatt catttacaaa
      121 cagcattgga taaagtgagg acgggggagt ttgagagcaa agactttaca cactatactt
      181 ttgatagtat caagcggaag taccaagtta gtggtaaaaa agtccttatg gataataact
      241 atagatttgg ttctggtata aaaaatacta gacaggatgc tattgctatt gtaaggttac
      301 tgcttgaaaa gaataaagat tatttaattt acatgaatgg cttagaagag ccaagtgttt
      361 tatataatcc agaaaataaa cgttataagt tcacaaataa caaaggaaaa gcaattggta
      421 atattccagt tggtttaact gcttttgaaa acagcgcaga aactcaagaa tatgtgttga
      481 aaaatattca gaaaaacgaa acgatttatc ttggtaatac ccgagtagat aatcctcgag
      541 taactgttaa taacaaaaaa agagatacta taggagttga atacggtaaa cgtattactt
      601 ataggattcc aatttattca aagcagctga ctgtcagagt tagccctaat tttgtagtag
      661 atagtactaa ttataattac cgactttctc aagcgcctat tattgcggaa gaaggtaaag
      721 agggacgttt gtatccaata gatagcagta ttagtgtcga tggtgataaa attgttttaa
      781 atagtagtgg tgatgaaaca gagcaaaaag aattattaca aaatagcctg tataaacttc
      841 actctattga taagttagac tttgatttga caaatgctga tattaaagag ttagagatta
      901 caggacatgt tgcttcaaaa agagattata atttgtctgt tgctcaggaa aagaaaggcg
      961 tcatagagac taaacggagt ctaatttctg ttgataatca aaaacaagat caagggattt
     1021 ttgtcgagga ttctaaaggc tatataaaga cacctagttt gagcagttat ggtataaatt
     1081 ttgccatgta tgacgacaat caaaatagat tattgcaagg ttcagagtat attttaggaa
     1141 gattgaatgc agataaaaaa gtatctctat atcaacctaa tggcttttgg ataaatactc
     1201 aaaaaacgat taagagttta aataaagaat atcttaacca aaatgcttac catattagag
     1261 gaaataa
//
LOCUS       DATRNX010000187         1212 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.190, whole genome shotgun sequence.
ACCESSION   DATRNX010000187 DATRNX010000000
VERSION     DATRNX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.190"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            358..>1212
                     /gene="ltrA"
                     /locus_tag="VBC81_001985"
     CDS             358..>1212
                     /gene="ltrA"
                     /locus_tag="VBC81_001985"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6912980.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCR"
BASE COUNT          390 a          238 c          288 g          296 t
ORIGIN      
        1 gcgtatatag cggatgaggg ggctataaac tctaaagtcc aaataggtag tcgtaaccta
       61 tatacgtaaa tcacgagagt aaacgaggaa agatgtccga cttatcccgt gaggtcctgt
      121 caagggtaaa acctagtaac aacgaacgac aagaagtcag ccgaacccat agtagtgatg
      181 aagtttctgt aatggaaatg gaacgaaggg gtgaacaact aatcgaagta atcctacttc
      241 acttgtgtct gtaaaacaaa cggtctgata gaactagatg cggttacgta ttgactagag
      301 gaaggttcac caatgtaaga tatcatgcag acaacgaaaa aagaaaggaa tacgcacatg
      361 tcagaattgc tagataagat atcatctcgg aacaatatgc tcgaagctta caagcaagtg
      421 aaatcaaaca aaggttctgc tggtatcgat ggggtcacta tcgagcagat ggatgactat
      481 cttcaccaaa attggcgaga aaccaagaaa ctcatcaaag agaggagcta taaacctcaa
      541 ccggttctca gggttgaaat cccaaaacca aacggaggag ttcgtaacct aggtatcccg
      601 acggctatgg atagaatgat tcagcaggcc atcgttcaag ttttgagtcc actctgcgaa
      661 aaacattttt cagagtatag ctatgggttc agacccaatc gctcctgcga aacagccatt
      721 gttcagctac ttgagtattt aaacgatggc tacgagtgga ttgtggacat tgacttggaa
      781 aagttcttcg atactgttcc gcaagacaga ttgatgtccc tggttcataa tatcattcaa
      841 gatggcgata cggagtcact gattcgtaag tacttccatt cgggagttgt tattaacgga
      901 cagcgacata agactttagt cgggacacct caaggcggga atctatcacc cctcctatct
      961 aatattatgc ttaatgagtt agacaaaggg ttggaaaagc gaggtcttcg ctttgtccgt
     1021 tacgccgatg actgtgtcat cactgtcgga agcgaagcag ctgctaagcg ggtcatgcat
     1081 tcggtcagta gctatattga gaagcgatta gggttgaaag tcaacatgac taagaccaag
     1141 attgtcagac cgaacaaact caaatacctc ggatttggtt tctggaaatc tccaaaaggt
     1201 tggaagtgtc gt
//
LOCUS       DATRNX010000188         1182 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.191, whole genome shotgun sequence.
ACCESSION   DATRNX010000188 DATRNX010000000
VERSION     DATRNX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.191"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(229..312)
                     /locus_tag="VBC81_001986"
     tRNA            complement(229..312)
                     /locus_tag="VBC81_001986"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Leu,seq:caa)
     gene            complement(323..394)
                     /locus_tag="VBC81_001987"
     tRNA            complement(323..394)
                     /locus_tag="VBC81_001987"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Gln,seq:ttg)
     gene            complement(401..473)
                     /locus_tag="VBC81_001988"
     tRNA            complement(401..473)
                     /locus_tag="VBC81_001988"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:His,seq:gtg)
     gene            complement(488..558)
                     /locus_tag="VBC81_001989"
     tRNA            complement(488..558)
                     /locus_tag="VBC81_001989"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Trp,seq:cca)
     gene            complement(565..645)
                     /locus_tag="VBC81_001990"
     tRNA            complement(565..645)
                     /locus_tag="VBC81_001990"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Tyr,seq:gta)
     gene            complement(657..729)
                     /locus_tag="VBC81_001991"
     tRNA            complement(657..729)
                     /locus_tag="VBC81_001991"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Phe,seq:gaa)
     gene            complement(732..805)
                     /locus_tag="VBC81_001992"
     tRNA            complement(732..805)
                     /locus_tag="VBC81_001992"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(769..771),aa:Met,seq:cat)
     gene            complement(816..905)
                     /locus_tag="VBC81_001993"
     tRNA            complement(816..905)
                     /locus_tag="VBC81_001993"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(867..869),aa:Ser,seq:tga)
     gene            complement(919..990)
                     /locus_tag="VBC81_001994"
     tRNA            complement(919..990)
                     /locus_tag="VBC81_001994"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Glu,seq:ttc)
     gene            complement(1001..1074)
                     /locus_tag="VBC81_001995"
     tRNA            complement(1001..1074)
                     /locus_tag="VBC81_001995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1038..1040),aa:Ile,seq:gat)
     gene            complement(1111..1181)
                     /locus_tag="VBC81_001996"
     tRNA            complement(1111..1181)
                     /locus_tag="VBC81_001996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1147..1149),aa:Gly,seq:tcc)
BASE COUNT          331 a          303 c          254 g          294 t
ORIGIN      
        1 ttattacttc agaacctatg acatttagga gtcctgtttg aatttcattt aaatgttgag
       61 tctccactaa ctcctgaaaa atttccttat tatttctgct tgttttaaaa cttcaataac
      121 aattgcaata atgaaaaaca aatataagta attttcagta actttttctt aaaattacca
      181 gtacaaaaca aaaaaagaca aggtttagaa accttgtctt ttaaagtata ccggcggccg
      241 gggtcgaacc ggcacgtccg tgaggacact ggattttgag tccagcgcgt ctgccaattc
      301 cgccacgccg gctatcttaa aactggggta gctggattcg aaccaacgca tgagggagtc
      361 aaagtccctt gccttaccgc ttggctatac cccatgaaaa ggcgagtgat gggaatcgaa
      421 cccacgaatg tcagagccac aatctgatgt gttaaccact tcaccacacc cgccatatta
      481 gaaaaaacac gggcagtagg aatcgaaccc acactgaagg ttttggagac cttagttcta
      541 cctttaaact atgcccgttt actatggaga gagagggatt cgaacccccg aacccgaagg
      601 agcggattta cagtccgccg cgtttagcct cttcgctatc tctcctaagg tataaatggc
      661 gcgagacgga atcgaaccgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
      721 taccgagcct attgcgggag caggatttga acctacgacc ttcgggttat gagcccgacg
      781 agctacctag ctgctccatc ccgcgatatc tttaaaggag gatgtgggat tcgaacccac
      841 gcacgctttt acacgcctga cggttttcaa gaccgttccc ttcagccgga cttgggtaat
      901 cctccatata acaaaaaata gtccgtacgg gattcgaacc cgtgttaccg ccgtgaaaag
      961 gcggtgtctt aaccccttga ccaacggacc atattcttga tgggcacgag tggactcgaa
     1021 ccaccgacct cacgcttatc aggcgtgcgc tctaaccacc tgagctacgc gcccaagcaa
     1081 atgcttggtt tttactttct tataaagtaa agcgggtgac gagaatcgaa ctcgcgacaa
     1141 cagcttggaa ggctgtagtt ttaccactaa actacacccg ct
//
LOCUS       DATRNX010000189         1169 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.192, whole genome shotgun sequence.
ACCESSION   DATRNX010000189 DATRNX010000000
VERSION     DATRNX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.192"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..310)
                     /locus_tag="VBC81_001997"
     CDS             complement(<1..310)
                     /locus_tag="VBC81_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912981.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     G"
     gene            complement(463..651)
                     /locus_tag="VBC81_001998"
     CDS             complement(463..651)
                     /locus_tag="VBC81_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6912982.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(648..941)
                     /locus_tag="VBC81_001999"
     CDS             complement(648..941)
                     /locus_tag="VBC81_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6912983.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            998..1147
                     /locus_tag="VBC81_002000"
                     /pseudo
     CDS             998..1147
                     /locus_tag="VBC81_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          417 a          233 c          108 g          411 t
ORIGIN      
        1 taccagaagc attataataa aagttcccta aatcaacatt caatttacca tcacttttaa
       61 catcatataa aacagtatac ttagcagacc cacctttaag tacaaaactt ttacttttag
      121 tatcaatatt agttactaca aagtttcctt cattatgata aattcctgat tcatcattaa
      181 tttctaaaac taacattttt tcaccatcat atactttata atactttaca tttaacgaat
      241 tagtcgaaca agcaactaaa aatactgtag atatagctaa aatcatcaat cctaataatt
      301 tctttttcat aactaaactt cctttgctat gagaaaatct tttaacctct caacatctct
      361 atcgcttaac ttacctataa ttttaatatt gaacccttta ttctcaacat cataatattg
      421 aatagtatct atccaagaac gcctatttaa atttgctttt gcctaatcaa taacttctaa
      481 atactgactt cttatattgt cagacttatt ttcatattta gtagtaaatc ttaaagtttt
      541 tattacttca tcattaaatt tgactactac agcaggacga acctttgaac ctgtaccatc
      601 agaatattca atacgagaaa ctaatacaga atatacctca atatcactca tttaaaatat
      661 tcctcatatt ctgaaaaatc ttcatctcca aaaaacgcat ttacactttc tggatcacta
      721 gccttaactc gtattccctt taattttgtt tctaccaaat tttgaaattc ttcatcagaa
      781 acagatgatt tattttttat ttcaaaagga atactttgag ttttaacaga ttgaatcaaa
      841 aacattttta ctgctgtgct catatccaac cctaaggatt gaaaaagttc tgtagcatca
      901 tcttttaatt gatcatcaac tcttactgct actgttgaca taatatcacc tcaaatcatt
      961 tttgtataac tattgtatca ctaaatgcaa tacaacgcaa acaaaaaagg aaatcgtctt
     1021 tcccaatgaa gaggctctag aatactatct agtcacacta tttaatgatt acaacttcaa
     1081 gcaagaacaa cgaatccaca aaggttttgg ccaatgtaca gacacacttg aaagcctatt
     1141 tgattaacaa tcccagcttt gccagtgtg
//
LOCUS       DATRNX010000190         1147 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.193, whole genome shotgun sequence.
ACCESSION   DATRNX010000190 DATRNX010000000
VERSION     DATRNX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.193"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            27..467
                     /locus_tag="VBC81_002001"
     CDS             27..467
                     /locus_tag="VBC81_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO6912984.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            460..804
                     /locus_tag="VBC81_002002"
     CDS             460..804
                     /locus_tag="VBC81_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912985.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT          429 a          148 c          210 g          360 t
ORIGIN      
        1 caaatataaa ataggtggtg caaataatga aaaaacatat aaaaagtatc ataccaatag
       61 ttcttattgg tatgatacta ggaggctgtc aaatgaatag tgaacataaa agtcagtata
      121 atgaaacaaa aagtagcaag caatcagaag tgaagaaaga taaaaaaatg acaaaaaaag
      181 aacaattagc ttatctcaaa gagcatgaac aagaaataat tgattttgta aaatctcaga
      241 ataaaaagat agaatctgta caaattgatt ggaatgatgt tcgatggagt aaagggggaa
      301 atggtacacc tcaaggagga ggagagggga ttttactttt tggggagatt aataatgatt
      361 ctgaatcaag ttggagagtt gatattgata tagaaaaagg acggctagac ctaaaaaata
      421 tgtatttagg acaacctata cgaattggag gtaaattatt tgagtaattg gaacttagaa
      481 agttttgata acctatttgc tactttagca gaatcaacat acacaaaaag accaagtaac
      541 tttacatata atgaattgaa tcctaagttg ctacgacaac tgaactcagg tagttctgtt
      601 gaattggatt tctcaaaatc tgttaagaaa ggaaaaaaag ttatcgaagg cggtcagaac
      661 ctccccaatg atggtattgt ctacctgcaa ccagataagt ccttaaaatc tattgatgaa
      721 aaagtagagg ttcgcgttcc tgatgtcaat ggcggttatc actctgagca ttgtgttact
      781 aattcctatc agaaaggtta ctgaaagatg atgaagcagt ggctctataa tcactaaaag
      841 ctcatcattg ctcagtgttt aagttaactt ctaaagctcg aaccttgtta aatcaaggtt
      901 tgtttacttt taaaagtgac attttatttt tctaaaaata aggttatcgg gattcctcat
      961 tctaagttac tttgtttata tgtaccttta cttaaggttt attttcatta tttttaattt
     1021 aatattttgg agtatgtacg ctccattata gtattttcga agacatcttt aaaagaactg
     1081 tagaagttaa aatattttaa cctagtatct aacgtcatat attggtacat agttttaaat
     1141 atccttt
//
LOCUS       DATRNX010000191         1115 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.194, whole genome shotgun sequence.
ACCESSION   DATRNX010000191 DATRNX010000000
VERSION     DATRNX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.194"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..315
                     /locus_tag="VBC81_002003"
     CDS             <1..315
                     /locus_tag="VBC81_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6912986.1"
                     /translation="ELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTG
                     NLDSVTAGEIINILKELAQDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLE
                     VE"
     gene            315..1115
                     /locus_tag="VBC81_002004"
                     /pseudo
     CDS             315..1115
                     /locus_tag="VBC81_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          476 a          132 c          207 g          300 t
ORIGIN      
        1 gaattaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc tggtggtcag
       61 caacaaaggg tagctattgc tagggcactg gtatcagatg ccccaataat actagctgat
      121 gagcctaccg gtaacctaga cagtgttact gctggagaaa taattaatat attaaaggaa
      181 ttagctcaag atagaaataa atgtgtaata gttgtaacac atagtaagga agtagcagat
      241 tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa taaaatgaat
      301 ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa agcttaaaat
      361 cattaattat tatattagtt attctatcaa tggcaacttt aagcatcatt agtttatcta
      421 ttaaagatgc cacagataga gcttctaagg aaacatttgc taatataaca aacagttttt
      481 ctatggaaat aaatagacaa gtaaatccag gaacacctag gggtggagga aatgtaaaag
      541 gtgaagatat taaaaagata tctcaaacaa acagtataga ctcctatgta aaaagaataa
      601 acagcgtcgc agacctagtt gatcatgata taattgaaac tcaagataca cttgcaaatc
      661 aatcacctga aagagcaaag aattttaaga gaacagttat gttaacaggg gttaatgact
      721 cagcaaaaga aacaaaattt gtatcagaag catataagtt agtagaagga aagcatttag
      781 aaaataaaga taaaaataaa attttaatgc ataaagattt ggcaaaaaaa aaacaatctc
      841 aaagttggag ataaaataaa aataaaatct aatttgtttg atgctgacaa tgaaaaagtt
      901 gcaaatgaaa ctgtagaagt agaaattaaa ggtttatttg atggtcataa tagcggtggg
      961 gtaagtgcag cacaagaact atacgaaaat acactaatca ctgacgttca ttcagctgct
     1021 aaagtttatg gtaatacaga agatacagcc gtataccaag atgcaacatt ctttgtaaaa
     1081 ggtgataaga atcttgatag tgtaataaaa gatct
//
LOCUS       DATRNX010000192         1091 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.195, whole genome shotgun sequence.
ACCESSION   DATRNX010000192 DATRNX010000000
VERSION     DATRNX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.195"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..1068
                     /locus_tag="VBC81_002005"
     CDS             <1..1068
                     /locus_tag="VBC81_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6912987.1"
                     /translation="QEWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMT
                     YARYFIPQYISADTVLYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGV
                     MVINNQRWRQENMTIKLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFD
                     MGAAIDGHKFIFDIPITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISS
                     KKVFGLDHPIKTQNYRLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISL
                     LAFENIRLHKTYTPPIIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNS
                     QLVPETVFASIDDLINYLQSI"
BASE COUNT          372 a          194 c          164 g          361 t
ORIGIN      
        1 caagaatggt ttttagctat gaaagatagg gttggacaaa ctggaaatca aattcaggat
       61 gtaaagctct tccatgatca cttatcccca aaatgggaaa ataaaaagct taatcatatt
      121 aattatatga cctatgctcg ttatttcata cctcagtaca tctcagctga tacagtttta
      181 tatcttgact ctgacttagt tgttactact aatttagata acctctttca aatttcacta
      241 gacaatgcat atttagctgc agttccagct ctttttgggc ttggatatgg gtttaatgct
      301 ggagtaatgg taattaacaa ccaacgttgg cgacaagaaa atatgactat taaattaatt
      361 gaaaaaaatc aaaaggaaat tgagaatgcc aacgaagggg atcaaacaat tcttaatcgc
      421 atgtttgaaa atcaggtaat ttatttagat gatacctaca attttcaaat tggttttgat
      481 atgggagctg ctatcgatgg gcataaattt atttttgaca tcccaattac cccactccca
      541 aaaattattc actacatttc gggaatcaaa ccttggcaaa cattatcaaa tatgagactc
      601 cgtgaggtat ggtggcacta taatttactt gaatggtcaa gtatcatatc tagtaaaaaa
      661 gtatttggtt tagaccaccc aattaaaaca caaaattatc gtctcaattt ccttattgtt
      721 acaacttctg attgtatacc atctatctca gaattagtca ctgcccttcc agattgtcta
      781 tttcacattg cagcaccaac agttatgtct gacattctta taagcctatt agcttttgaa
      841 aatatccgcc ttcataaaac ttacactcca cctattattg ataaattatt agcagaagct
      901 gatatttatc ttgatattaa tgtttttgga aaaatggagg gcattctaca gaaaatgtca
      961 caatctgaaa aaccaattct tactacaaga gaatgcaata gccagcttgt tccagaaaca
     1021 gtttttgcat ctatcgatga tttaattaat tatcttcagt ctatataaaa aaattggcat
     1081 ccattcacta t
//
LOCUS       DATRNX010000193         1069 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.196, whole genome shotgun sequence.
ACCESSION   DATRNX010000193 DATRNX010000000
VERSION     DATRNX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.196"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..>1069)
                     /gene="dnaN"
                     /locus_tag="VBC81_002006"
     CDS             complement(<1..>1069)
                     /gene="dnaN"
                     /locus_tag="VBC81_002006"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6912988.1"
                     /translation="ISHKNAIPILSTVKIEVTRDAIILTGSNGQISIENTIPASNENA
                     GLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQHQIVLTSGKSEITLKGKDVDQY
                     PRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTGVHLVISQNKYFKAVATD
                     SHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETVEVFFSDSQMLFRSENIS
                     FYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYLISNATQNGTVRLEIQNE
                     TVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESLKAVKSETVTIRFISPVR
                     PFTLTPGEDTEDFIQLITPVRT"
BASE COUNT          349 a          177 c          185 g          358 t
ORIGIN      
        1 gtacgaacag gagttattaa ttggatgaaa tcttcagtat cttcaccagg ggtcaaagta
       61 aatggacgta ctggagaaat aaatcgaatc gtaactgttt cgctttttac tgcttttaaa
      121 gattcaatta ggtaagttgg attaaaacta atatttaaac tatcaccttt aaggctaaca
      181 gtatccaatt cctcattaac tttaccaact tctggagagt ttacatgagc tgagactgtt
      241 tcattttgaa tttctaaacg aacagtaccg ttctgagttg cattcgaaat taaataagca
      301 cgttccatag catggcgtaa agcatttgta ttaaagataa tttcggtttc aaactgatta
      361 gttaggaggc gatcagtatc agggtagttt ccttcaagca aacgtgtata gaagctgata
      421 ttttcacttc taaataacat ttgactatct gagaagaaaa cctctacagt ttcaatatca
      481 tctgtaaaaa cagccgaaaa ttctcgaagg gatttacttg gaacaaccaa atcaaaatta
      541 ttagccgatt tctctaattg gaaagtgcgt tgactcatac ggtgtgaatc tgtcgcaaca
      601 gccttaaagt atttattttg actgataacc aaatgaacac ctgttaagat tggacggctt
      661 tcttgttggc tagcagcaaa agcagtttca ttaataattg attttaacag tttagtttct
      721 aatgttaatg gagtatctgt tgtcatttcc tgcagacgag ggtattgatc gacatcctta
      781 cctttcaaag taatctctga ttttccacta gtaagaacaa tttgatgttg ttcaatctct
      841 gtaaattcta aagttacatc tggtaaactt gaaataatat taataaagaa accagcttct
      901 aacaaaatag agcctggatt cgttactagt aaacctgcat tttcatttga agcaggaata
      961 gtattttcaa ttgaaatttg tccatttgac cccgttaaaa tgatagcatc tctagtcact
     1021 tctattttaa cagttgaaag gattggaatc gcatttttat gactaatag
//
LOCUS       DATRNX010000194         1059 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.197, whole genome shotgun sequence.
ACCESSION   DATRNX010000194 DATRNX010000000
VERSION     DATRNX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.197"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(12..1034)
                     /locus_tag="VBC81_002007"
     CDS             complement(12..1034)
                     /locus_tag="VBC81_002007"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6912989.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
BASE COUNT          320 a          228 c          177 g          334 t
ORIGIN      
        1 ttagtataga cttaatctac tttataaatc cacccttctg gtgcttcaac atcaccaaac
       61 tggatcccta ctaactcatc atatagttta cgtgtcgctg gaccaacttc tgtctcactg
      121 tagaagacat ggaagtcatc tccgttttga attcctccaa ttggtgaaat aactgcggct
      181 gtaccacatg cgccagcttc tgtgaattta tctaattcgt cgacaaagac atctccttca
      241 atcgcttcca taccaaacct ttctttagca aggtaaagca atgaatattt agtaattgaa
      301 ggtaaaattg aaggactcaa aggggtgata aactgattat ctttagtaat tccaaagaaa
      361 ttagctgctc caacctcttc aattttagta tgtgtcgctg gatcaagata gataacatct
      421 gaaaattgtt tctcatgagc gtatttccca ggaagaagac tagcagcata gttaccacca
      481 acttttgcag ctcctgtacc attcggtgct gctctatcat attctttgga aacaataaag
      541 ttagttggtg ttaaaccacc tttaaaataa ctacctactg gcattgcaaa taccgtaaaa
      601 atatattctt cagcaggttt aactccaata atatctccca caccaattaa taagggacga
      661 atgtataatg ttgcccctgt accatacggt ggaacaaact cctcattagc gcgtacaaca
      721 gatttaaccg ctgcaataaa tttgtctgtc ggaacatgtg gcattaaaag acgatctgct
      781 gttctttgaa gtcgttctgc attttgatct ggacgaaata actgaataga gccatcttta
      841 gttctataag ctttcaatcc ttcaaaagct tgttgcccat agtggagggc aggagaactt
      901 tctgaaatat gtagcgttgc atcatctgtc aacttgccat catcccattt cccatcttta
      961 aaatgcgaaa tgtatcggaa tggtaatttt ctgtaagcaa aacctaaatt atcccaatct
     1021 aaatttactg tcataaggta cctctttctt tgggtttct
//
LOCUS       DATRNX010000195         1046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.198, whole genome shotgun sequence.
ACCESSION   DATRNX010000195 DATRNX010000000
VERSION     DATRNX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.198"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..542
                     /locus_tag="VBC81_002008"
     CDS             <1..542
                     /locus_tag="VBC81_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEO6912990.1"
                     /translation="LQEHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNNGDKG
                     PYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQKLYKKYQNKLPLKALKDKLMQS
                     LTTKGFDYQIGHTVIQNLEIEKDQELEDDLIYKELDKQYQKLSKKHDQYELKQRIINV
                     LMRKGYQYEDIKSALREYL"
     gene            626..>1046
                     /locus_tag="VBC81_002009"
     CDS             626..>1046
                     /locus_tag="VBC81_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6912991.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYI"
BASE COUNT          434 a          161 c          168 g          283 t
ORIGIN      
        1 atcttcaaga acatgatatt gattctaaaa ttattccaca aattattgat aatctaaaaa
       61 gtgagaattg gataaacgat aaaaactacg tgcaatcttt catacagcaa aatctaaata
      121 atggcgataa ggggccatac gtgataaaac aaaaattatt gcaaaaaggt atcaaaagta
      181 aaataattga atctgaactt aaagctataa actttcaaga cttagcatcc aaaataagcc
      241 aaaaactcta taaaaaatat caaaacaaac ttccactaaa agcattaaaa gataaattga
      301 tgcagagctt aactacaaaa ggatttgatt atcagatagg gcataccgta attcaaaacc
      361 ttgaaattga aaaagatcaa gaacttgaag atgacctcat ttataaagaa ttagataaac
      421 aataccaaaa attatcaaaa aaacatgacc agtatgaatt aaagcaacgt ataatcaatg
      481 tcttaatgcg taaaggatac cagtacgaag atatcaaaag tgctttaaga gaatatttat
      541 aagataataa ctctcaaacg atgtatttta cagaaaatta tgataaacta taacagacgt
      601 ataaattgta gaaagttggt aggctatgag attacctaaa gaaggcgact ttattacaat
      661 tcaaagttac aaacatgatg gtagtttaca ccgaacttgg cgtgacacca tggtattaaa
      721 aacaaccgaa aatgccctca ttggtgttaa tgatcatact ttagtaacag aaaatgatgg
      781 tcgacgctgg gtgacacgag agcctgcaat agtatacttt cataaaaaat attggtttaa
      841 cattatcgct atgatacgtg aaactggtgt ctcctactat tgtaatctag caagtccgta
      901 tatcttggat cctgaagcac tcaagtacat cgactacgac cttgatgtca aagtatttgc
      961 agatggtgaa aaaagactac tggatgtgga cgaatatgaa cagcataaag ctcagatgaa
     1021 ctatcctacc gatattgatt atatat
//
LOCUS       DATRNX010000196         1017 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.199, whole genome shotgun sequence.
ACCESSION   DATRNX010000196 DATRNX010000000
VERSION     DATRNX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.199"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(396..614)
                     /locus_tag="VBC81_002010"
                     /pseudo
     CDS             complement(396..614)
                     /locus_tag="VBC81_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          320 a          179 c          138 g          380 t
ORIGIN      
        1 ctgaagtgtg taggcgaggt ctaagtaaag aatttaagcg ttttgataat aaaaaagtga
       61 gaacaataga ttctcactca caattagcct ggtaatttct cacacgattt ggtactgctt
      121 tttttaagtt gggcgatggt gttattttta atatgagtag ctgcactcga atccgattgc
      181 tcaaaagcac ttgtcgctcc attgagatag ctattcagat cagaaataac tggttgaatg
      241 ttttggctga cttctttgat aacttttgat ccagcttcta agtattttca atgttcttga
      301 acccattggt gatctttttt tactaagtca catgctgtta aaatcatcat taagcttatc
      361 ccttccacaa taataatcaa ttttttaaat cctttttatc ttattgattt tttcaatttt
      421 attgctgtct tgacgttgct atcgcaaaca accaactcaa tgcaatatag ttacggtata
      481 catatcaatg taagactcaa gatttttaaa ttcatattat ttgtaggttc tttatcagac
      541 caagctaata ctctttcatt tgaagtaata ttattatttg tatgatattc aagattagct
      601 atttttgatt tcacaaaaaa accttcttct acttttttag actgattctt tcttcccact
      661 tcttcgtatc aatatcatat atttgaagaa ctaaactctc agagtttcca gtcaatttta
      721 gtaataatcc caaaactaat ccttcctaca aaccaacgga taattttatt tcaactggtc
      781 ctggttttaa ttttacttgt gcaatttcgc ttgttatacc aactattgat atcgccgata
      841 ctcctattcc taatgatgcg cacgcatcat tagttaaaca aagtacttta aatcctacag
      901 agggtcattc catcaattaa tggcataggt tttggcacta gaaactgaat atgaattcta
      961 ttatctatta aaaaaatcaa tatcaccaga gtatttataa aacataacga taaaaaa
//
LOCUS       DATRNX010000197          997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.200, whole genome shotgun sequence.
ACCESSION   DATRNX010000197 DATRNX010000000
VERSION     DATRNX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.200"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..975
                     /locus_tag="VBC81_002011"
     CDS             <1..975
                     /locus_tag="VBC81_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6912992.1"
                     /translation="FAVTLLIKCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHI
                     ACVILALAIGTTALKNAIALALLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAK
                     ADYSEMDVIMTKDHKLVVIHDDNLKRLSGMNKDVSKLTLDQVTKIPIHQGYFASYIPS
                     LAEFMKTAQSLDQKIMIELKPYNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEK
                     VEKKLPQLDTGYLIPLHWGTLQNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINR
                     DNLMIRYLQSPVNGIITDKLNLFKVINKDIKNSPNYYQRALQLIDSEG"
BASE COUNT          373 a          177 c          152 g          295 t
ORIGIN      
        1 tttgccgtaa cacttcttat taaatgttta atcataagtc acctattagc agatattaaa
       61 caaaataaaa gtattgttca acaatggaga gcttggaaag gcgtccacca taagcaaaaa
      121 tttaaacaca ttgcttgtgt tattctagct ctagctattg gtaccactgc cttaaaaaat
      181 gctatagctc tggctctctt acaagataac atttcaatta aaaaagaatt aatagcccat
      241 cgtagtgata catcacatgg cgttgaaaat tctattgaag cattaaaagc agcccataaa
      301 gcaaaagcag attattcaga gatggatgtt atcatgacaa aagatcataa attagtagtc
      361 attcatgatg ataatctcaa acgactctct ggtatgaaca aagatgtttc taaactaact
      421 ttagatcaag ttacaaaaat cccaattcac caagggtatt ttgctagtta cattccatca
      481 ttggcagaat ttatgaagac cgctcagtca ctagatcaaa aaatcatgat tgaattgaaa
      541 ccgtataatc aaaacctcga tatctatgcg gatgaattta tcaaagaatt taaagagctt
      601 cgtctatcaa ccaagcataa agtaatgtct ctcaacttga cactcattga aaaggttgag
      661 aaaaaattgc cacaacttga tacaggttac ctaatcccac tacattgggg aacgcttcaa
      721 aatcataacg ttgactttta tgggattgag gaattttcat acaatgattg gattgcatat
      781 ctcgcccaag aatataataa acagctctat gtttggacta ttaaccgtga taaccttatg
      841 atacgttatt tacaaagccc agttaatgga atcatcacag ataaactcaa tcttttcaag
      901 gtcattaata aagatatcaa aaatagccct aattattatc aaagagctct ccaacttatt
      961 gatagcgagg gttaaattaa ataaaaagct tagtaga
//
LOCUS       DATRNX010000198          975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.201, whole genome shotgun sequence.
ACCESSION   DATRNX010000198 DATRNX010000000
VERSION     DATRNX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.201"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            121..432
                     /locus_tag="VBC81_002012"
     CDS             121..432
                     /locus_tag="VBC81_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6912993.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            453..>975
                     /locus_tag="VBC81_002013"
     CDS             453..>975
                     /locus_tag="VBC81_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912994.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFL"
BASE COUNT          370 a          166 c          148 g          291 t
ORIGIN      
        1 aatcatcgtc tatcactctt attttctacc ggtaacttag ttatatttga catctcaact
       61 actttgtatt acaatgtagt taaccagtaa cttgtattac aatatagata aggatataat
      121 atgattccat cgcaaatgct aaaaggttta cttgaaggag ctatcctaaa aatcatacag
      181 caagaggaga cttatgctta tgaaatctca aaaaaattaa attcttatgg ttttggggaa
      241 atttccgaag ggacaatcta ccccattatc ttaaggctac aaagcaataa atatataaaa
      301 ggacaacaaa agccttcccc tcaaggacct aaacgtaaat attatacgtt aaccaatgaa
      361 ggcgtggagc aattagccca atttaaacag aattgggagt cacttagtaa agcattaaac
      421 aaactactct aaaaattgga gaggaaacta gcatggataa actgaaaaaa ttacaaaaag
      481 aaaatacgct attagaagga agaattgata actctaataa ccaaacctat acagatatga
      541 ttgtctattt gcgaggtgct agcattagcc cctaccacca ggagctcatt cgaaatgata
      601 ttgtcaatat gttattagaa gcacaagagc gacaagcaag tttagttagt gtatttggag
      661 aagataggca cgactttata aaccaagtta ttaaatctac acctaaaatc tctaagaaag
      721 aagaaacatt acaacgttgg gatttagcca ttttactact aactatccaa atgattatct
      781 ttttaggagg atatctcatt actgaagcac ttcaacagtc agtaccagat ctaatcccta
      841 ttactctatt agatgttctt tttgcaatct ttatcagtat tattgctgtc aagataactg
      901 ataccataat atatgctaca tataacttcg ataaatcgaa agaaaagaaa tatttctttc
      961 gatatatgtt tctaa
//
LOCUS       DATRNX010000199          939 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.202, whole genome shotgun sequence.
ACCESSION   DATRNX010000199 DATRNX010000000
VERSION     DATRNX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.202"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            135..>939
                     /locus_tag="VBC81_002014"
     CDS             135..>939
                     /locus_tag="VBC81_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6912995.1"
                     /translation="MNKKHKLLKNIEEFKTITQKRLTERGKFPYDTVHSTFEIKDENF
                     IMERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKDSSYSSNINISEDK
                     IKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEAYRARNVDTEYYRA
                     SDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISHAAVRAYYNRSWHI
                     IDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVKYMESN"
BASE COUNT          389 a          120 c          154 g          276 t
ORIGIN      
        1 aaggttatta caaaaacctt atcaatttag aagaaattaa aaatatccaa gctacattaa
       61 aaaaagaaag aaatgaagaa tatgtctttg tcaaattaaa tgggaaaatc gcacatgata
      121 caacagtatt cttgatgaat aaaaaacata aattacttaa aaatatagag gaatttaaga
      181 cgataaccca aaaaaggcta actgagagag gaaagttccc atatgatacc gttcattcta
      241 cgtttgaaat taaggatgaa aactttatca tggaaagatt gaaatcatca gggttatcta
      301 tgggtaaacc agttgattat atgggcgtaa atggtatccc tatttacact aaaacattat
      361 ctatagataa taagtttgcc tttgaaaata attcaaaaga ttcttcatat tcttcaaaca
      421 ttaatattag tgaagataaa ataaaagaaa atgaccaaaa aattctagat ttaatagtta
      481 aatctggagc taataatcaa aacttaacag atgaagaaaa agtaattgct ttcactaaat
      541 atataggtga aataactaat tatgacaatg aagcttacag agctagaaat gtcgacacag
      601 aatattacag agcttcagat ttattttctg taacagaaag aaaacttgct atgtgcgttg
      661 gctatagtgt aacagccgct agagcattta atattatggg gatacctagt tatgtcgttt
      721 ctggtaaaag tcctcaagga atatctcatg cagctgtgag agcgtattat aatagaagtt
      781 ggcatatcat agatatcaca gccagtacat attggaaaaa tggtaattac aaaacaacgt
      841 attccgattt tattaaagaa tactgtatag atggttatga cgtttatgat cccgcaaaaa
      901 caaataatag atttaaagtt aagtatatgg aatcaaatg
//
LOCUS       DATRNX010000200          937 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.203, whole genome shotgun sequence.
ACCESSION   DATRNX010000200 DATRNX010000000
VERSION     DATRNX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.203"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            147..863
                     /locus_tag="VBC81_002015"
     CDS             147..863
                     /locus_tag="VBC81_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6912996.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          346 a          147 c          162 g          282 t
ORIGIN      
        1 ctcaaataag ccattttatt gactattata gtatattaac ttcctgtcaa ttcttttatt
       61 gacaagcctg tctttacaca ctataatata tgatatcaaa ttggtgagta aacactcact
      121 cactgagaga ggagcaaaaa gttatcatga aaaaagtcat cgatttaaaa aaactacaaa
      181 aagcatacgc ctcagaaact gttttaaata atattaattt ggaggtgttt aaaggagaaa
      241 taattggatt aataggaccc tctggagcag ggaaatctac cttgattaaa actatgcttg
      301 gcatggaaaa agcagataag ggaacagctc ttgttcttga tactcaaatg ccagatcgta
      361 atattttaaa tcaaattggc tatatggctc aatctgatgc cttacacgag tctttaactg
      421 gcttagaaaa tttattattc tttggaaaaa tgaaaggtat tcaaaaaact gaattaaaac
      481 agcagataac tcatatttct aaagtagtag atctagaaaa ccaacttgat aaatttgtct
      541 caggttactc aggaggtatg aaaagacggc tttctctagc catcgcccta cttggaaacc
      601 ccacagtttt aatcctagat gaacctaccg ttggaattga tccatccttg aggagaaaaa
      661 tctggcaaga gctaattaat attaaggatg aaggacgttc tatctttatt acaacccacg
      721 ttatggatga agcagaatta acaagtaagg ttgcactact attacgtgga aacattattg
      781 cctttgatac tccattacat ttaaaaaaac aatttaatgt gagtactatt gaggaagttt
      841 tcttaaaagc tgaaggagaa taatcatgag aattattgca ataactgaaa aggttataaa
      901 agaactgttt cgtgataaaa gaacacttgc tatgatg
//
LOCUS       DATRNX010000201          842 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.204, whole genome shotgun sequence.
ACCESSION   DATRNX010000201 DATRNX010000000
VERSION     DATRNX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.204"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(149..703)
                     /gene="raiA"
                     /locus_tag="VBC81_002016"
     CDS             complement(149..703)
                     /gene="raiA"
                     /locus_tag="VBC81_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6912997.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
     gene            complement(780..>842)
                     /locus_tag="VBC81_002017"
     CDS             complement(780..>842)
                     /locus_tag="VBC81_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6912998.1"
                     /translation="IRKLLEEKGIKNIKSFSLAR"
BASE COUNT          239 a          145 c          107 g          351 t
ORIGIN      
        1 acttttttta agttgtttat aaaatgataa tacaatatta ggttcgctta agaactcatt
       61 tagtatacta taatttttta tttgttgtca ataggtttta aaaaaatctc agaaaaaact
      121 ctgagatttt ttaaattatg ttacaaagtt aatttccttt agcttcaatt aaacctagtt
      181 cgccatcttc acgcttgtaa aggacatttg tcgtattatc ttctgcatct gtatagataa
      241 agaaatcatg acctaaaagt tccatttgca acaatgcttc ctcaacatcc attggtttta
      301 gattaacatt cttagtacgt acaattcttt ggcttactgc ttcttcatct ggctcagcct
      361 caaattctgt tgtgaaaact tgacttgctg gaattttttc acgatatttt ttcgcaattt
      421 tagttttatt tttacgaatt tgacgctcaa ttttatcaac aactaagtca attgacccat
      481 acatatcttg tgaaacatct tctgctcgta aagtaataga atctattaag attgttactt
      541 caacttttgc ggtcttctct ctgtatactt tgaggttgac tctagtatct aattcttgtt
      601 cttcattaaa gtatttttca actttagaga gtttggtctc aacatactca cgaatagctt
      661 ctgttacttc gatgttttca ccacgaatac tgtatttaat catatgagta cctctttctt
      721 gcgttgttaa cgctttctat attcttatta taaccgcttt catgaaaatt tgcaagattt
      781 tagcgtgcta atgaaaaact ttttatattt tttatacctt tttcttctaa tagtttcctg
      841 at
//
LOCUS       DATRNX010000202          803 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.205, whole genome shotgun sequence.
ACCESSION   DATRNX010000202 DATRNX010000000
VERSION     DATRNX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.205"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            188..261
                     /locus_tag="VBC81_002018"
     tRNA            188..261
                     /locus_tag="VBC81_002018"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:Arg,seq:tct)
     gene            complement(334..>803)
                     /gene="rlmH"
                     /locus_tag="VBC81_002019"
     CDS             complement(334..>803)
                     /gene="rlmH"
                     /locus_tag="VBC81_002019"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6912999.1"
                     /translation="IITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTPDKAS
                     ISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYSTITFV
                     IGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
BASE COUNT          258 a          142 c          123 g          280 t
ORIGIN      
        1 gttaggtgtt tccttagtac tcttaatatt atatcagaaa aaaaaggttt gtctatactt
       61 ttgttcaata atttttacaa aaaaattgaa cctacttatg agcaaaaaaa tatatttcca
      121 actcataaat agtacatatg aaaacttgac agcctgccta atattttggt agaataacag
      181 agttgatggt tccatagctc agctggatag agcattcgcc ttctaagcga acggtcgcag
      241 gttcgaatcc tgctggaatc agataatgaa agtctagata gattatctag gcttttttat
      301 ttgtttttag cggttgtttc ataaatggaa cgattatttg tggtaaggag tgccttgacg
      361 aatcataaaa gcacgataaa tttgttccat caagactaat ttcataagtt gatgaggtaa
      421 ggttaacagt ccaaaactca ttaaataatc agcacgtttt ttcacttttt gagataaacc
      481 caaactacca ccaataacaa aagtaatagt agagtagccg gatatcattt tttgatcgat
      541 tagatggcta aatgattcag atggcaattg tttcccttct atagccaaac caatgacata
      601 atccctctca cctattttac tcaaaatacg ttccccttct atatctaaaa ttctttgatt
      661 ttcagatata ctagctttat caggtgtctt ttcatcagct agctcaattg tctcaatttt
      721 agaaaagcga ttcagacgtt tctgatactc tgcaacacct tctttaaggt atttctcttt
      781 caattttcca actgtaatta ttt
//
LOCUS       DATRNX010000203          778 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.206, whole genome shotgun sequence.
ACCESSION   DATRNX010000203 DATRNX010000000
VERSION     DATRNX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.206"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..169
                     /locus_tag="VBC81_002020"
     CDS             <1..169
                     /locus_tag="VBC81_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913000.1"
                     /translation="STKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNV
                     NDIEVIYMKKE"
     gene            171..680
                     /locus_tag="VBC81_002021"
     CDS             171..680
                     /locus_tag="VBC81_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913001.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
BASE COUNT          300 a           90 c          156 g          232 t
ORIGIN      
        1 ttctactaaa attaatgaac aattttcgat tagtttttct gaagatagaa ttggtggtaa
       61 acttagagca ttaggatatc aaccgaatga aataggtttt tcaaaggaca tcaatagtaa
      121 taatcaaaat gttaatgata ttgaagtgat ttatatgaag aaagaataga atgatttcag
      181 aagaaagtta cagatattta gttgaggatg cttatcgagt tgattcaaaa aaagtaaaaa
      241 tacctctgaa aagaagtgat attgtaggga acagtgacta tgttattatt gaaccaccta
      301 tagataatac ttcaaatggt atgcaagcta tggtagttgc tccgataaaa gaaggaatga
      361 cagctgaacc agatactagt gaaatagtga ttgtttatgc aggtactaat ttaggtgagc
      421 gtttagacat agctacagat gttgaaatgg ttgctggagg ggatacatat ttacttgctg
      481 atcctaaaac caagactttt aggaaatctc agggaaaatc agcactagag tacgcagaaa
      541 aaatatcgag taaatatcct aattcagaaa ttactacaac cggtcactcc ttaggggaat
      601 cagaggcact atatgtcgct ttaaaaatgg gttggatgaa tgttggttat aatggtccag
      661 atattcataa catgatataa aatgacgaaa tagattatat gaaatctcat ccagaataat
      721 ttagaaaaaa atcgaaaaat atgagttgtt tgagttaaaa ttttaactat ggagatag
//
LOCUS       DATRNX010000204          741 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.207, whole genome shotgun sequence.
ACCESSION   DATRNX010000204 DATRNX010000000
VERSION     DATRNX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.207"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            11..733
                     /locus_tag="VBC81_002022"
     CDS             11..733
                     /locus_tag="VBC81_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6913002.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT          290 a           93 c          149 g          209 t
ORIGIN      
        1 taacatctaa atgagacttg ataaattttt agtagaatgc ggacttggtt cacgtactca
       61 agtcaaatta atattgaaaa aaaagcagat tagtgttaat agtaatagcg agacatctcc
      121 aaaagtacag gtagatgaat atagagatga gattaaatat aatggaactc tagtgtcata
      181 tgaaaagttt gtttattata tgcttcataa acccaaaggt gttatatcag caacggatga
      241 tccttcgcat aaaaccgtat tagatttgct agacaaaact gctcgagata aagctgtttt
      301 tccagtgggg cgtttagata ttgatacaac aggccttttg ttaataacta ataatgggga
      361 attagcgcat aaaatgttat cacctaaaaa acacgttgat aaatgctatg aggcaaaaat
      421 ctcagggata atgacagaag acgatatttt ggcatttgat aaagggatta ttctgaaaga
      481 ttttacttgt ttaccagcat tattagaaat tgtggaagta aatcaggtga agaagcagag
      541 tcttgttaaa atcactatta aggaaggaaa attccatcaa gttaaacgta tggttgctgc
      601 gtgtggcaag gaagtcttag agctaaagcg tctaagtatg ggaaatctac aattagataa
      661 acagctagaa agtggacaat ggagacggct aacaataaaa gaaatagaaa aattagaaaa
      721 acatatgcaa taatataaaa t
//
LOCUS       DATRNX010000205          690 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.210, whole genome shotgun sequence.
ACCESSION   DATRNX010000205 DATRNX010000000
VERSION     DATRNX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.210"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBC81_002023"
     CDS             complement(<1..684)
                     /locus_tag="VBC81_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6913003.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          213 a          130 c           91 g          256 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt
//
LOCUS       DATRNX010000206          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.211, whole genome shotgun sequence.
ACCESSION   DATRNX010000206 DATRNX010000000
VERSION     DATRNX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.211"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBC81_002024"
     tRNA            complement(78..151)
                     /locus_tag="VBC81_002024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBC81_002025"
     tRNA            complement(163..247)
                     /locus_tag="VBC81_002025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBC81_002026"
     tRNA            complement(256..327)
                     /locus_tag="VBC81_002026"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBC81_002027"
     tRNA            complement(361..433)
                     /locus_tag="VBC81_002027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBC81_002028"
     tRNA            complement(444..525)
                     /locus_tag="VBC81_002028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBC81_002029"
     tRNA            complement(535..607)
                     /locus_tag="VBC81_002029"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBC81_002030"
     tRNA            complement(610..680)
                     /locus_tag="VBC81_002030"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATRNX010000207          624 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.214, whole genome shotgun sequence.
ACCESSION   DATRNX010000207 DATRNX010000000
VERSION     DATRNX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.214"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(104..>624)
                     /locus_tag="VBC81_002031"
     CDS             complement(104..>624)
                     /locus_tag="VBC81_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6913004.1"
                     /translation="GFIYPNKKNNKGRKDIFILTNSDQIEHLEVLVHHLSDYHFHIAA
                     YTEMSFKLMSFSQEQNVTLYPNISRTDLDNLFEICDIYFDINHGNEVDDVIRRAFEYN
                     HLIFAFDNTCHNRELVLDSNIISHTTCEQLINLMKNLSGSIMYLLEQQREQTSNETKE
                     RYKEILGGYGNA"
BASE COUNT          199 a          103 c           79 g          243 t
ORIGIN      
        1 taccaaataa aaagtaggtc gcttgtcatg ttcactctct ggttctttat atctaagttt
       61 atcaatactc attttaattt ctttattgag taattcattt cttttaggca tttccatacc
      121 ctcctaatat ttctttataa cgctcttttg tttcattact tgtttgttct ctttgttgct
      181 ctagcaaata cataatggag cctgataaat ttttcattaa atttatcaat tgttcacagg
      241 ttgtgtgaga aatgatattg ctatctaata ctaactctct gttatgacag gtattatcaa
      301 aagcaaagat aaggtgatta tattcaaatg cccttctaat aacatcatca acttcatttc
      361 catgattaat gtcaaaataa atatcacaga tttcaaataa gttgtctaaa tcagttcttg
      421 aaatattcgg atatagcgtt acattttgtt cttggctaaa tgacatcaac ttaaaagaca
      481 tttctgtgta cgctgcaata tgaaaatgat aatcagacaa gtgatgaacc aacacttcta
      541 aatgttcaat ttggtcagag tttgtcaaaa taaaaatatc tttcctaccc ttattgttct
      601 ttttattagg atatataaag ccta
//
LOCUS       DATRNX010000208          620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.215, whole genome shotgun sequence.
ACCESSION   DATRNX010000208 DATRNX010000000
VERSION     DATRNX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.215"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            34..620
                     /locus_tag="VBC81_002032"
                     /pseudo
     CDS             34..620
                     /locus_tag="VBC81_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088186184.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT          218 a           95 c          140 g          167 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga agaaaatgag cgcttgagaa cggagaatgc ctttctaaaa
      481 aagttgaggg atttgcgctt gagggacgaa gccttacaga gcgaacggca gaaacaatta
      541 gagaaatggt cacaggagga ttccgactag acctcttact tgagataact aagatagctc
      601 gtgcaactta ctattatcaa
//
LOCUS       DATRNX010000209          601 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.216, whole genome shotgun sequence.
ACCESSION   DATRNX010000209 DATRNX010000000
VERSION     DATRNX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.216"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(27..518)
                     /locus_tag="VBC81_002033"
     CDS             complement(27..518)
                     /locus_tag="VBC81_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6913005.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT          168 a           85 c          108 g          240 t
ORIGIN      
        1 aaggggcttt tttatattaa gcaatactag attaagtttg ttttcgagtt attagtaata
       61 tctgcgatag caagccaaat ttccatttga taatcagtat cttgtggatg ttgagatgaa
      121 taaatttcta agtttggcga gttagctggt tttagattat tctcttgaaa aaatccttgt
      181 attatttttc gccatgtttc ttgaagactc gatggcattg gacctttaac agaaatgact
      241 gcataggtag cgcttgggat cctaataatt tctaaccctt ttggggcttg gacacttgtc
      301 tctacttgat aagtggcact gtaatcgaaa cgaaaatcag gttgtgtttt acaaatgcca
      361 atggcttgct ggataccttt ttcttttagt tttgatggat tatattgttt atgtaatttt
      421 tcccaaagtt taggacattc ttgatttgac tttacatttt ctaaagttac tccagcaagt
      481 atcattgctg gcttttcaac aatttgaaat gatagcataa aaatctttat tctttatgat
      541 tccttaagta attatagagt cgttaaaata gtttcttttt agaataattg tcttgttagc
      601 a
//
LOCUS       DATRNX010000210          537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.219, whole genome shotgun sequence.
ACCESSION   DATRNX010000210 DATRNX010000000
VERSION     DATRNX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.219"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..>537
                     /locus_tag="VBC81_002034"
     CDS             <1..>537
                     /locus_tag="VBC81_002034"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6913006.1"
                     /translation="ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHD
                     MGHNKSNSHGIDTLIKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDER
                     LPEIISAMRDKDLLLITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFAD
                     ISATVADNFGVDTAMIGES"
BASE COUNT          156 a          109 c          110 g          162 t
ORIGIN      
        1 gccaaccgtc acgactatgc agtatcacca tttgaagata ccgtattaaa taaactagat
       61 caagctggca tcgatactta tgctgttggt aaaatcaatg atattttcaa tggttcaggc
      121 attaatcatg atatgggaca taacaaatcg aatagtcatg gtattgatac tttgataaaa
      181 acaatggggt tatcagagtt tgaaaaaggc ttctccttta ctaatttggt tgactttgat
      241 gctctctatg gtcaccgtcg tgatcctcat ggctatcgtg attgtcttca tgagtttgat
      301 gagcgcctcc cagagatcat ttcagcaatg cgtgacaaag acttactttt gatcactgcc
      361 gatcatggta atgacccaac ttatgcaggt acagaccata caagagaata cattcctctc
      421 ttagcctata gcccatcttt cacaggaaac ggccttatcc cagtagggca tttcgcagat
      481 atttcagcaa cagttgcgga taactttggt gttgatactg cgatgattgg agagagc
//
LOCUS       DATRNX010000211          516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.221, whole genome shotgun sequence.
ACCESSION   DATRNX010000211 DATRNX010000000
VERSION     DATRNX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.221"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..>516
                     /locus_tag="VBC81_002035"
     CDS             <1..>516
                     /locus_tag="VBC81_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO6913007.1"
                     /translation="EKLLVESREGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDL
                     EVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMN
                     QSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIETAKHIQVIKRTLY
                     DNKRDFFELFPV"
BASE COUNT          172 a          115 c          102 g          127 t
ORIGIN      
        1 gaaaaacttt tagttgaatc tagagaagga atccctttga acaaagagac cttctggaaa
       61 atagatagag tgctttctaa tggggtcaag aagggccaac gtatctatca tatcctcaaa
      121 accaacgatc tagaagtgag ttcttcaacc gtttatcgac acatcaagaa aggctaccta
      181 tccatcacac caatcgacct acccagagct gtgaagttca aaaaaaggcg aaagagcaca
      241 ctccctccta ttccaaaagc gattaaagaa gggcgacggt acgaggattt tatagaacac
      301 atgaaccaat cagagctgaa ctcctggctt gaaatggaca cggttattgg acggattggt
      361 ggaaaggtcc ttctcacctt caatgtcgcc ttctgtaact tcatctttgc caaactgatg
      421 gattctaaga cagctatcga aactgctaaa cacatacagg tcattaagag gacactctat
      481 gataacaaga gagacttctt cgaactcttt cctgtt
//
LOCUS       DATRNX010000212          452 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.227, whole genome shotgun sequence.
ACCESSION   DATRNX010000212 DATRNX010000000
VERSION     DATRNX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.227"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(<1..>452)
                     /locus_tag="VBC81_002036"
     CDS             complement(<1..>452)
                     /locus_tag="VBC81_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6913008.1"
                     /translation="KDKAIKSDIQSIYDEHRGNYGYRRIYLELRNRGFVINHKRVQGL
                     MKSMGLTARIRRKRKYASYKGEVGKKADNLIQRQFEGSKPYEKCYTDVTEFALPEGKL
                     YLSPVLDGYNSEIIDFTLSRSPALKQVQTMLERAFPAASYSETILHSD"
BASE COUNT          125 a          106 c           93 g          128 t
ORIGIN      
        1 gtcgctgtgg agaatggttt cgctgtatga agccgcagga aaagccctct caagcatggt
       61 ttgaacctgt ttcaaggcag gagaccgaga cagggtaaaa tcaattatct cactgttata
      121 gccatcaaga acaggcgata gataaagttt cccttcaggt aaagcaaact ccgtcacatc
      181 ggtatagcac ttctcatagg gcttcgaccc ctcaaactgg cgttgaatca ggttatcagc
      241 cttcttacca acctcacctt tgtaagaggc atacttgcgc ttacgacgaa tacgagcagt
      301 caagcccata cttttcatca agccttgcac ccttttgtgg ttgatgacaa agccacgatt
      361 tcgcagttct aaataaatcc gacgatagcc atagtttcct ctatgttcat cataaatgga
      421 ttgaatgtca gatttgattg ctttgtcttt at
//
LOCUS       DATRNX010000213          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.231, whole genome shotgun sequence.
ACCESSION   DATRNX010000213 DATRNX010000000
VERSION     DATRNX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.231"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBC81_002037"
     CDS             <1..380
                     /locus_tag="VBC81_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6913009.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATRNX010000214          395 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.234, whole genome shotgun sequence.
ACCESSION   DATRNX010000214 DATRNX010000000
VERSION     DATRNX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.234"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            74..322
                     /locus_tag="VBC81_002038"
     CDS             74..322
                     /locus_tag="VBC81_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO6913010.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
BASE COUNT          156 a           69 c           61 g          109 t
ORIGIN      
        1 aaatttatct caaaaagtga taaagggttg ccaaaaatca cttaattaga tatacttata
       61 tagttagatt aatatggagg tgaaaacatt ggcaaatatt aaatcagcta tcaaacgcgc
      121 tgaacttaac gttaaacaaa acgaaaaaaa ctcagcacaa aaatcagcta tgcgtactgc
      181 tattaaagca tttgaagcta acccttcaga agaactttac cgcgctgctt cttcaagcat
      241 cgacaaagct gcttctaaag gtttaattca cacaaacaaa gcaagccgcg ataaagcacg
      301 tcttgcaact aaacttggtt aattaaccta gaaaacatcg gatatatcga tgttttttat
      361 ttaatttttt cgtagaataa aagactagga gaaat
//
LOCUS       DATRNX010000215          393 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.denovo.235, whole genome shotgun sequence.
ACCESSION   DATRNX010000215 DATRNX010000000
VERSION     DATRNX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.denovo.235"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            62..>393
                     /locus_tag="VBC81_002039"
     CDS             62..>393
                     /locus_tag="VBC81_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913011.1"
                     /translation="MNSESKSQSNEVKNSKQSAVKKGKKVIKKEQVAYLKKYEKELTE
                     YVKTQNPDISEIKYDWNSVRIVEASNGTPQGREQLLLIWGYANGSELTNFTLNFTLGD
                     NQMPNIDSV"
BASE COUNT          164 a           50 c           74 g          105 t
ORIGIN      
        1 aaaatatgta aaatggttaa ttcccatatc tattattggg atgttactag caggttgtca
       61 aatgaatagt gaatctaaaa gtcagtcaaa cgaagtaaaa aatagcaaac agtcagcagt
      121 aaagaaaggt aaaaaagtaa taaaaaaaga acaagtagct tatctcaaaa agtatgaaaa
      181 agaattaact gaatatgtca aaacccaaaa tccagatatt agcgaaatta agtatgattg
      241 gaatagtgtg cgtatagtgg aagctagtaa tggaacccct caaggcagag agcagctatt
      301 attaatttgg ggttatgcaa atggcagtga attaaccaat tttactctaa atttcacatt
      361 gggtgataat caaatgccta atattgactc agt
//
LOCUS       DATRNX010000216        16863 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.guided.039, whole genome shotgun sequence.
ACCESSION   DATRNX010000216 DATRNX010000000
VERSION     DATRNX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.guided.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            complement(98..379)
                     /locus_tag="VBC81_002040"
     CDS             complement(98..379)
                     /locus_tag="VBC81_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913012.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
     gene            complement(413..799)
                     /locus_tag="VBC81_002041"
     CDS             complement(413..799)
                     /locus_tag="VBC81_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913013.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            complement(784..1464)
                     /locus_tag="VBC81_002042"
     CDS             complement(784..1464)
                     /locus_tag="VBC81_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913014.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            complement(1410..1997)
                     /locus_tag="VBC81_002043"
     CDS             complement(1410..1997)
                     /locus_tag="VBC81_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913015.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            2406..2720
                     /locus_tag="VBC81_002044"
     CDS             2406..2720
                     /locus_tag="VBC81_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6913016.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2736..3122
                     /locus_tag="VBC81_002045"
     CDS             2736..3122
                     /locus_tag="VBC81_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6913017.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            3151..4536
                     /locus_tag="VBC81_002046"
     CDS             3151..4536
                     /locus_tag="VBC81_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6913018.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4539..4691
                     /locus_tag="VBC81_002047"
     CDS             4539..4691
                     /locus_tag="VBC81_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6913019.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4714..5919
                     /locus_tag="VBC81_002048"
     CDS             4714..5919
                     /locus_tag="VBC81_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6913020.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            5962..6183
                     /locus_tag="VBC81_002049"
     CDS             5962..6183
                     /locus_tag="VBC81_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6913021.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6300..6797
                     /locus_tag="VBC81_002050"
     CDS             6300..6797
                     /locus_tag="VBC81_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6913022.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6772..7278
                     /locus_tag="VBC81_002051"
     CDS             6772..7278
                     /locus_tag="VBC81_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6913023.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7262..9709
                     /locus_tag="VBC81_002052"
     CDS             7262..9709
                     /locus_tag="VBC81_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6913024.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9712..11889
                     /locus_tag="VBC81_002053"
     CDS             9712..11889
                     /locus_tag="VBC81_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6913025.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11886..12887
                     /locus_tag="VBC81_002054"
     CDS             11886..12887
                     /locus_tag="VBC81_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6913026.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            12884..13819
                     /locus_tag="VBC81_002055"
     CDS             12884..13819
                     /locus_tag="VBC81_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6913027.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            14064..14180
                     /locus_tag="VBC81_002056"
     CDS             14064..14180
                     /locus_tag="VBC81_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6913028.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14196..16115
                     /gene="tet(M)"
                     /locus_tag="VBC81_002057"
     CDS             14196..16115
                     /gene="tet(M)"
                     /locus_tag="VBC81_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6913029.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            16234..16401
                     /locus_tag="VBC81_002058"
     CDS             16234..16401
                     /locus_tag="VBC81_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6913030.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(16461..16814)
                     /locus_tag="VBC81_002059"
     CDS             complement(16461..16814)
                     /locus_tag="VBC81_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6913031.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         5324 a         3093 c         3376 g         5070 t
ORIGIN      
        1 ccttcatgta aaatgttatg tcttctaatc caacttgatt attttcatat ctataataac
       61 tatcaggata gtaagcttcc tcaacatttt cactcgatta taaatcatca taactacctt
      121 tatttaaatc tacctgtgcc tttacttttt cccaaaattg tcctacaagt ccagtgtcaa
      181 tttggttttt tatcgttttt attgatgatt tagcagtctc agaaatatca tcaatctttt
      241 cactataagt cgtcttaata gaatcagctt cttctactat ttccggggca tggacaaacc
      301 ttactaaagc tttttgaatt tgctttttga attctttgtc ttctttggtt cctttcaata
      361 cagcgcctat taaactcatt tattgcctct ttcactttat agtccatcta aattagtagt
      421 attttcttcc ttctaaacta gaaatgtcat tttctaattt tactcggtct tttagaaact
      481 gtgtccgact attttcctct tggacaatga tagtctgttt atagttttca tattcatcaa
      541 ttaattccat ttgataactt tcaattattc gatttaaact cataaaagtc gaaccatctg
      601 aatcattttg tctcaaaaag tgacttgtct cttcatagac ttcttgtaaa taagtctcta
      661 actcgttctt tttatcctca aggctatata atttatcttc tatctccata ttttcgcgat
      721 gataattatc ttccaatttc cctaggttta tttttagact attaattttg ttttcatttt
      781 gatctagtat tgtgaccatt ccttaaattc tcctgctaat ttggagtcct cttctagcaa
      841 tttttggaca ccagattcaa tttgtttttg aagattagta tatgaagttg tgatctcttg
      901 tgcattagta actttttcat caaagaaagt ttcaattggt gttactatgg tgtcctctct
      961 aactccaccc gcactatatg catcagctaa ttcttctgga cttaaatctg ttataccagg
     1021 aggcatcact ttagtttttg caaatagttc tgatgctttt tctttagcta attcagcatt
     1081 cttcttgata tcatcagaag ctgtttgaga tgccgtggtt aaaccgttag caataaatat
     1141 agcctgttca gaatctaaat atactttttc actatgacta agtttgcctc cagatttttt
     1201 taatttagct ttagcttttt gaaaatgaac ttcttcataa agaacagact gaacagctgg
     1261 tgacttagga tttacgatgt tacctttttc atcttttaac tgaccatcat cagtaaattt
     1321 ccaactctct attttatgac ttttaccatt tacccatttc acggtaccat aacctttatc
     1381 atctagttta ttaaaatcgt tccaccaagt tacattatcc tctttatgtc gaaaattagc
     1441 aaaatattca ggatgcttag ccatgaattt ttttcatctt catttaaagt gctataccta
     1501 aaccatccat taaatactgt agtcgggata tgatatttag cacctaattt tagcatataa
     1561 gcagattgag aatagcctga aagctgagtc acagtccatt tgtcatgtga ctgtacgtct
     1621 tttaaaaatt tatcagctga ttttaactga gtcgaacttt gatgtggttg agcagatgaa
     1681 actgcgccaa ttaaatcttt ataattactt gaatccgttg cggcggaaat aactgcaaca
     1741 ctgttataat ctactttacc cttgataatt ggtgcaactg ccataccctg atagccagac
     1801 atatcgtcag gagaagtttt taatacctta aacgctttac ccgtctttgt tactattaaa
     1861 tttgtttcat tagatttttt tggattagct gttattattt ttccgccatt ttgtttcaca
     1921 tagtttggat ttacactata tacgttctca ccttgattca atctatttaa atcatactcg
     1981 ctataagaat atgtcattat cttttttcct tttctcctgt agaaaactca actaaaatat
     2041 gattttttgt tcttccatat ttttaattta aaaatagcat aaaaatctag ttatccgcat
     2101 aaaaactgga cttatcacac tttatcaagg tcaaaaccac tcaatttact actaatttac
     2161 tacttatgaa tgagctttga tacgacgatt tatccttgaa aagtgaagat ataaagatac
     2221 ttccaataaa atttgaatat ttaataggta gacacttcaa aaaatgaggt gtctattttt
     2281 ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa aatcaagaat caaaaggtcg
     2341 ttccccactc tttaagacca tcaaacattc attcagccaa taaaaaagaa aggataggta
     2401 aaaatatgga acttaaattt gtgattccca acatggaaaa aacattcggc aatttagaat
     2461 ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg acggctaact gtcttatcaa
     2521 gaagctataa tctctattct gatgttcaaa gagcagatga tattgtggtg gtgcttcctg
     2581 ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt gaagttagtc aatccacgta
     2641 ttaccgcaga gggctacaaa atcggcactc gtggttttac aaattacctt ttacatgctg
     2701 acgacatgat aaaagaataa agaaagagag gaaaaatgat gagattagca aatggcattg
     2761 tattagataa agacacgact tttggagaat tgaaattctc tgctctacgt cgtgaagtga
     2821 gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa ggaacgtacc tatgacttaa
     2881 aatccaaagg acaaggacgc atgattcaag taagtattcc tgccagcgtg cctttgaaag
     2941 agtttgatta taacgcacgg gtggaactta tcaatcccat tgcggacacc gttgctactg
     3001 ccacctatca aggagcagat gttgactggt atatcaaggc agacgatatt gtgctgacaa
     3061 aggattctag ttcattcaaa gctcaaccac aagcaaagaa agaaccgaca caagacaaat
     3121 agtcgctagg tagaaaggag actttttcgc atgaaacagc gtggtaaaag gattcgccca
     3181 tctggtaaag atttagtctt tcattttacg atagcgtcac tcctgcctgt tttcctgctg
     3241 gttgtcggac tgtttcatgt gaagacaatc cagcagatca actggcagga ttttaaccta
     3301 tcacaagcag ataagattga cattccctat ttaattatca gtttcagtgt cgcaattctt
     3361 atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct atgatacggt taaacaactt
     3421 taccaccgtc aaaaactggc aaagatgata cttgaaaaca agtggtatga atctgaacag
     3481 gtcaaaacag agggtttctt taaagatagt gctggtcgta caaaggaaaa gataacctac
     3541 ttccctaaaa tgtattatcg acttaaaaat ggcttgatac agatacgggt ggaaatcacg
     3601 ctgggaaaat atcaagacca actcttacac ttggaaaaga aattagagag tggcttgtac
     3661 tgtgagctga cggataaaga gttaaaggat tcctatgtgg aatatacttt gctctatgac
     3721 accatagcca gtcgtatttc tattgatgaa gtagaagcta aagatggtaa acttcgctta
     3781 atgaaaaacg tatggtggga atatgataag ctccctcata tgttgattgc tggtggtaca
     3841 ggtggcggta aaacttactt tatactgaca ctgattgaag ccttgcttca tacagattca
     3901 aaactgtata ttcttgaccc gaaaaatgct gaccttgcgg acttaggttc tgtgatggca
     3961 aatgtctact atagaaaaga agacttgctt tcttgcattg aaacattcta tgaagaaatg
     4021 atgaaacgta gtgaggaaat gaagcagatg aagaactata agactggcaa aaattatgct
     4081 tacttaggtc tcccggcaca cttcttaatc tttgatgaat acgtcgcttt catggaaatg
     4141 ctgggaacaa aagaaaacac cgcagttatg aataagctga aacagattgt catgttaggt
     4201 cgtcaagctg gcttctttct aatactggct tgtcaacgtc cagacgcaaa atatttaggc
     4261 gacggaatcc gtgatcagtt taatttcaga gtggctttag gtcgtatgtc tgaaatgggc
     4321 tatggcatga tgtttggcag tgacgtacaa aaggatttct tcttaaagcg aatcaaaggt
     4381 cgtggctatg ttgatgtagg aacaagtgtc atatcagagt tttatactcc ccttgtacca
     4441 aaaggatatg atttcttgga ggaaattaaa aagttatcca acagcagaca gtccacgcag
     4501 gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt gctggctggt gtggcaatag
     4561 ccacgccagc acttaacccc ccgtatctaa caggggggta caaatcgaca ggaaacagtc
     4621 aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag cgtatgtcaa
     4681 atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact gaatgaacaa
     4741 acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca aaaccgttta
     4801 gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag
     4861 ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa tcccgacgct
     4921 ccccttgaaa tgctgtttga ttatgtaagg attcgctttc cgacaacaga cgtacagcag
     4981 gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga ctatggtttc
     5041 tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc ccatgaactg
     5101 gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga aagctatctt
     5161 ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc tggcggtgtg
     5221 atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttgaatat ccctgtactc
     5281 actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa aagctatcgc
     5341 agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct ctatatcggt
     5401 tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca gtacaagaaa
     5461 aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat
     5521 gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga gcataccgcc
     5581 tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa acctcgttct
     5641 gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga acgattaaaa
     5701 ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct atctcatcaa
     5761 gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta
     5821 aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt gaagcaacag
     5881 tcagtaaaag aacaggacgt gataacaaca aaaaaataac tcaaatacaa attcattgaa
     5941 tatagagagg agaacatttt tatgaatttt ggacaaaacc tttataactg gtttctatca
     6001 aacgctcaat cactggtgct tttagcaatc gttgtgattg gcttgtatct tggcttcaag
     6061 cgtgagttta gcaaactgat tggcttttta attattgcga ttattgcggt tggcttagtc
     6121 ttcaacgctg ctggagtaaa agacatttta ctagagctat tcaatcgcat tattggtgct
     6181 taaataaaac cgttcttttg tggaatataa gtggttttct tatgttccgc aaaggaatgg
     6241 tacaccaaac gaagtgcggt agggattttt gaatctctac aaagaaagga cgtgaatata
     6301 tggacgatat gcaagtctat attgcgaatt taggcaaata caatgagggc gaattggtcg
     6361 gtgcgtggtt tacctttccc attgactttg aggaagtcaa agagaaaatc ggcttgaatg
     6421 atgaatatga ggaatacgcc attcatgact acgagttacc ctttacggtt gacgaataca
     6481 cttccattgg cgaactcaat cgactatggg aaatggtatc ggaattaccc gaagaattac
     6541 aatcggagct atctgctctg ctcactcatt tttcaagcat tgaagaacta agcgaacatc
     6601 aagaggatat tatcattcat tccgattgtg atgatatgta tgacgtggca cgctactaca
     6661 ttgaagaaac gggtgcttta ggcgaagtac cagctagtct tcaaaactat attgattatc
     6721 aagcctatgg tcgggattta gacctttcag gaacgtttat ctcaaccaat catgggattt
     6781 ttgaaatcgt ctattaaatc tgtcggtaca ttactactgg cagattttct attttacggg
     6841 gtggctcaat cagctacccc tattttttat gaaaggattg attacatgaa gaaaatacga
     6901 agctatacca gtatctggtc tgtggaaaag gtactgtatt ctatcaatga ttttagactt
     6961 ccgtttccca taacctttac gcaaatgaca tggtttgtcg tgtcactctt tgcagtgatg
     7021 atacttggca acttgccccc tctttccatg atagagggag catttctcaa atactttggg
     7081 attcctgtgg ctttcacatg gtttatgtct acaaaaactt ttgatggtaa aaagccttat
     7141 ggatttttga agtctgtcat tgcttatgca ctgcgaccaa agctgaccta tgcaggaaaa
     7201 aaagtaacgc ttggcagaaa ccagccacaa gaagccatta cagcagttag gagtgaattt
     7261 tatggcatat ccaattaaat acattgaaaa caatctcgtc tggaataaag acggggaatg
     7321 ttatgcttac tatgagcttg ttccttacaa ttactcattt ctaagtccag aacagaaaat
     7381 acaagtgcat gattctttca gacagcttat cgcacaaaat cgtgatggca aaattcatgc
     7441 tttacaaatc agtacagaat ccagcatacg ttctgcacaa gagcgttcca aaaatgaagt
     7501 cactggcaag ctcaaagcgg ttgcctatga caaaatcgac caacagacag acgctttaat
     7561 atccatgatt ggcgaaaatc aagtgaacta ccgtttcttt atcggcttta agttgcttct
     7621 caacgatcag gagttttcta tgaaaagtct taccgttgaa gcaaaaaatg ctttgtctga
     7681 ttttgtctat gatgtgaacc ataagctgat gggcgatttt gttagtatga gtaatgatga
     7741 aatcctgcgt tttcagaaga tggaaaagct cttagaaaat aaaatctctc gtcgtttcaa
     7801 aatccgcagg ttagataagg acgacttcgg ctatctgatt gaacaccttt acggacagac
     7861 aggcactgcc tatgaagagt atgagtacca tctatcaaag aaaaagctgg ataatgaaac
     7921 gctgattaaa tactatgact tgattaagcc tactcgctgt ttggtggaag aaaaacagcg
     7981 atatttgaaa atccagcagg aagatgaaac cgtctatgta gcttacttta ccattaacag
     8041 cattgtcgga gaactggact tcccgtcctc tgaaatcttc tactaccagc aacagcaatt
     8101 tacattcccg attgatacgt caatgaatgt ggaaattgta gcgaatcgta aagccctatc
     8161 tactgtccgc aataaaaaga aagaactgaa agacttggat aaccacgctt ggcaaagtga
     8221 taatgaaacc agctccaatg tggcggaagc tctggaaagt gtgaatgagc tggaaaccaa
     8281 tttagaccaa agcaaggaat ctatgtacaa gctgtcttat gtggtaaggg tatcagcaaa
     8341 tgatcttgac gaactcaaac gtcgttgtaa tgaagtgaaa gatttttatg acgatttaag
     8401 cgtaaaactg gtacgaccat ttggggatat gctcggctta catgaagaat ttttacctgc
     8461 cagcaagcgt tatatgaatg attatattca atacgtgacc tctgatttcc tcgctggttt
     8521 aggttttggt gctactcaaa tgctggggga aaatgagggg atttatgttg gctacagctt
     8581 agatactgga cgcaatgtct atctgaaacc tgctcttgcc agtcaagggg ttaagggttc
     8641 agtaaccaat gcgttagcgt cggcttttgt tggttcgctg ggtggtggta aatcctttgc
     8701 gaataacctt atcgtctatt atgcggtgct ttatggggca caagcagtga ttgtagaccc
     8761 aaaagcagaa cgtggcagat ggaaagaaac cttgccagag atttcccatg aaatcaatat
     8821 cgtcactctg acttctgatg agaaaaacaa aggcttactt gacccttatg tgattatgaa
     8881 aaatcccaaa gattctgaat cactggctat tgatattctg acattcctta cggggatttc
     8941 ctctcgtgat ggggaacgct tcccaatcct tagaaaagcc attcgtgcag taaccaatag
     9001 tgaagtacga gggttgatga aagtgattga ggaattacgg gttgagaata cgccactaag
     9061 taccagtata gccgaccata tcgaaagttt tacagactat gactttgcac atttattatt
     9121 cagtaatggt tatgtggagc agtctatcag cttagaaaaa caactgaaca ttatacaggt
     9181 tgcggacttg gtacttcccg acaaggaaac ttcctttgag gaatatacca ctatggagct
     9241 tttatccgtt gctatgctga ttgtcattag tacctttgct ttagacttta tccatacaga
     9301 ccgaagcatt ttcaagattg tagatttaga cgaagcatgg agctttttac aggtagcaca
     9361 aggaaaaaca ctatctatga agctggttcg ggctggtcgt gctatgaacg ctggggtata
     9421 tttcgtgacc caaaatacag acgacctctt agatgaaaaa ctgaaaaata acctcggctt
     9481 aaaatttgca tttcgttcca ctgaccttaa cgagattaaa aagaccttag ccttttttgg
     9541 tgtagaccca gaggacgaaa acaatcagaa gcgattgcgt gatttggaaa acgggcaatg
     9601 ccttatcagt gatttatatg gtcgtgtcgg tgtgatacag ttccaccctg tatttgaaga
     9661 actgctccat gcctttgata ccagaccacc tgtgcgaaaa gaggtgtaaa tgtgaaacca
     9721 tcaatagtaa acagaataaa atcaaactgg acgctgaaac gtctaggtaa agtggcaatg
     9781 acagtggctt tcacacttgt gattgccatt tttcttttag ccatgctggg aacggtggtt
     9841 caagctgcgg gcttggtaga tgatacggtc aatgtggcaa atgaatacag ccgataccca
     9901 cttgaaaact atcaactgga tttttatgtg gataatagct ggggctggct tccgtggaac
     9961 tggtcggacg ggattggaaa acaggtcatg tatggactat atgccattac caattttatt
    10021 tggacaatca gtttgtatgt ttccaatgcg acaggttact tagtacagga agcctattcc
    10081 ttagacttca tttccgctac agcagattcc attggtaaga atatgcagac cttagctggt
    10141 gtgagtgcaa acggattttc aacagagggt ttctatgttg gattcctctt actcttgatt
    10201 ttggttcttg gggtttatgt tgcctatacg ggactgataa agagagaaac cacaaaggca
    10261 attcatgcca ttatgaattt tgtgctggtg tttatcctat cggcttcctt tattgcctac
    10321 gctcccgact acattaaaaa aatcaatgac ttttcatcag acatcagtaa tgccagttta
    10381 tcacttggca cgaagattgt catgccccat tccgatagtc aaggcaagga cagcgtggac
    10441 ttaatcagag atagcctgtt ttccatacag gttcagcaac cgtggctact gcttcaatac
    10501 aacagttcag acattgaaag tatcggtatt gaccgtgtgg aaagcctgct ctccaccagc
    10561 ccagattcca acaatggcga agacagagaa aaaattgttg cggaagaaat tgaagacaga
    10621 agcaatacca atctaaccat tacaaagacc attaaccgtt taggtacagt cttcttccta
    10681 tttgtcttca atattgggat ttccatattt gtattcctat taacaggaat catgattttc
    10741 tcgcaggtac tttttatcat ctatgctatg tttctgcctg tgagctttat tttaagcatg
    10801 attccatcat ttgatggtat gtcaaaacga gccataacaa agctctttaa taccattttg
    10861 acacgagctg gaatcacatt gattattacg acagcattta gtatttcaac catgctctat
    10921 accttatcgg ctggttatcc gttctttttg attgcttttc tacagattgt gacctttgca
    10981 ggaatctact tcaagctggg cgatttaatg agtatgtttt ctctacagag taacgattct
    11041 caaagtgtgg gaagtcgtgt gatgagaaaa cctcgtatgc ttatgcacgc tcacatgcac
    11101 cgtctacagc ggaaacttgg acgttccatg actactctag gggctgggtc tgccattgtt
    11161 acaggtaaaa aaggacagtc gggttcgggg agttctgcaa ggacacaagc agatcactcc
    11221 cgaccagacg gaaaggaaaa atcaacactt ggaaaacgta tcggtcaaac catcggtaca
    11281 gtagctgata ccaaagacag aatggtagac actgctagtg gtttgaaaga acaggttaaa
    11341 gatttgccga ccaatgcaag atatgcagta tatcaaggaa aatccaaagt aaaagagaat
    11401 gtccgtgatt taaccagtag tatttctcaa accaaagcgg acagagccag tggacgcaag
    11461 gaacagcagg aacaaaggcg aaaaaccatt gcgaagcgtc gctctgaaat ggaacaggtc
    11521 aaacagaaaa aacagcctgc ttcttctgtt catgaaagac cgactacaag acaagaacaa
    11581 tatcatgatg aacagacctc aaaacagtct aatattcaga cttcatataa ggaatctcaa
    11641 caagccaaac aagagcgtcc agcagttaag tccgattttt caagtccaaa agtggaacgc
    11701 caaggcaata ccgttcaaga aaaaaccgtt caaaagccag caacttcaac cactacagca
    11761 gatagaactt cacaacgtcc aatcacaaaa gaacgtccgt ctactgttca aagagtacca
    11821 ctacaaaata caagaagtag accaccaatc aaaaccgcca ccattaagaa agtcggtaag
    11881 aaaccatgaa gttgaaaact ttagtgattg gtggttctgg attattcttg atggtcttct
    11941 cactgcttct gtttgttgcc attttatttt cagatgaaca ggacagcgga atttccaata
    12001 ttcattatgg aggtgtgaat gtttccgcag aagtgctggc tcataagcct atggtagaaa
    12061 aatatgccaa agaatatggc gttgaagaat atgtcaacat acttcttgcg attatacagg
    12121 tggaatcggg cggtactgcg gaagatgtta tgcagtcctc ggaatccctc ggtcttccac
    12181 ctaattcatt gagtacagaa gaatccatta agcaaggtgt gaagtatttc agtgaattat
    12241 tagccagtag cgaaaggctc agtgtagatt tagaatcggt tatccagtcc tacaattatg
    12301 gtggtggttt cttagggtat gtggctaatc gtggaaataa atataccttt gaactggctc
    12361 aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc ttaccccaat cccatagcca
    12421 tacctatcaa tgggggctgg cgatacaact atggcaatat gttttatgtg caactggtaa
    12481 cgcagtatct tgtcacaaca gagtttgatg atgatacggt acaagccatc atggacgaag
    12541 cactgaaata tgagggctgg cgatacgttt acggtggagc ttccccgact acttcttttg
    12601 attgtagcgg actgacacaa tggacgtatg gaaaagctgg aattaactta ccacgaaccg
    12661 cacaacagca atatgatgtg acccagcata tcccactatc ggaagcacaa gctggcgatt
    12721 tggttttctt tcattctacc tataacgctg gctcttatat tactcatgtt gggatatacc
    12781 ttggcaataa ccgtatgttt catgcaggcg acccaatcgg ttatgccgac ttaacaagcc
    12841 cctactggca acagcattta gtgggagcag gacgaatcaa acaatgagaa aggaagattt
    12901 aatgatgaaa tttagaaaaa atcagaataa agaaaaacag ataccaaagg aaaagaaacc
    12961 tcgtgtctac tataaggtca atcctcataa aaaggttgtg attgccttgt gggtactttt
    13021 agggcttagt ttcagctttg cgatattcaa gcactttaca gctatagata ctcatactat
    13081 tcacgaaaca actatcatag aaaaggaata cgttgatact catcatgtag aaaattttgt
    13141 agagaacttt gcgaaagtct actattcatg ggagcaatcc gataagtcca ttgataatcg
    13201 aatggaaagt ctaaaaggct atctgacaga tgaacttcaa gctctcaatg ttgatacagt
    13261 acgcaaagat attcctgtat cgtcttctgt aagaggattt cagatatgga cggtagagcc
    13321 aactggcgac aatgagttta atgtaaccta cagtgtagac cagctcatta cagagggaga
    13381 aaatacaaag accgtccact ctgcttatat agtgagtgtc tatgtagatg gttctggaaa
    13441 tatggtactg gttaagaatc cgaccattac caacatacct aagaaatcaa gttataaacc
    13501 aaaagccatt gaaagtgagg ggacggttga ttccattaca accaatgaaa tcaatgagtt
    13561 tttaacgacg ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt
    13621 gaatgacggg atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc
    13681 tattcacaat cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca
    13741 gcagaccaaa gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa
    13801 ttggaagatt atagaataac aaatattggt acattattac agctattttg taatcacgta
    13861 ctctctttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta
    13921 agtatctatt taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa
    13981 tttgatagtg ggaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc
    14041 cagtttaaga atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt
    14101 gtatgcctat ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt
    14161 atgccctttt gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt
    14221 tagctcatgt tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag
    14281 cgattacaga attaggaagc gtggacagag gtacaacgaa aacggataat acgcttttag
    14341 aacgtcagag aggaattaca attcagacgg cgataacctc ttttcagtgg aaaaatacta
    14401 aggtgaacat catagacacg ccaggacata tggatttttt agcagaagta tatcgttcat
    14461 tatcagtatt agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa
    14521 ctcgtatatt gtttcatgca cttaggaaaa taggtattcc cacaatcttt tttatcaata
    14581 agattgacca aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt
    14641 ctgcggaaat tgtaatcaaa cagaaggtag aactgcatcc taatatgcgt gtaatgaact
    14701 ttaccgaatc tgaacaatgg gatatggtaa tagaaggaaa tgattacctt ttggagaaat
    14761 atacgtctgg gaaattattg gaagcattag aactcgaaca agaggaaagc ataagatttc
    14821 ataattgttc cctgttccct gtttatcacg gaagtgcaaa aaacaatata gggattgata
    14881 accttataga agtgattacg aataaatttt attcatcaac acatcgaggt cagtctgaac
    14941 tttgcggaaa agttttcaaa attgagtatt cggaaaaaag acagcgtctt gcatatatac
    15001 gtctttatag tggcgtactg catttgcgag attcggttag aatatcggaa aaggaaaaaa
    15061 taaaaattac agaaatgtat acttcaataa atggtgaatt atgtaaaatc gataaggctt
    15121 attccgggga aattgttatt ttgcagaatg agtttttgaa gttaaatagt gttcttggag
    15181 atacaaagct attgccacag agagagagaa ttgaaaatcc cctccctctg ctgcaaacga
    15241 ctgttgaacc gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct
    15301 ccgacagtga cccgcttctg cgatattatg tggattctgc gacacatgaa atcatacttt
    15361 ctttcttagg gaaagtacaa atggaagtga cttgtgctct gctgcaagaa aagtatcatg
    15421 tggagataga aataaaagag cctacagtca tttatatgga aagaccgtta aaaaaagcag
    15481 agtataccat tcacatcgaa gttccaccga atcctttctg ggcttccatt ggtctatctg
    15541 tagcacagct tccattaggg agcggagtac agtatgagag ctcggtttct cttggatact
    15601 taaatcaatc gtttcaaaat gcagttatgg aggggatacg ctatggctgt gaacaaggat
    15661 tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc
    15721 ctgttagtac cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa
    15781 aaaaagctgg aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg
    15841 aatatctttc acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc
    15901 aattgaaaaa taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat
    15961 atcgtagtga tttaacttcc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag
    16021 ggtaccatgt tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag
    16081 ataaagtacg atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata
    16141 tggtttcttg ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag
    16201 gaggagatag ttattataaa ctacaagtgg atattgtgtc ctgtatgtgg aaataaaaca
    16261 cgattaaaga taagggaaga tactgaatta aaaaaattcc ccctctattg tccgaaatgc
    16321 agacaagaaa atttaattga aataaagcag ttcaaagtaa ctgtgattac agagccagac
    16381 gcaaagacgc agagccgata aaatgagatt aatacaatct cattttatcg gctctttccg
    16441 ttatgtatgg attcttttaa ttagtcttcg atgtttcttg cttcgttgat accgctggct
    16501 aaagattcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt ctctatctgt
    16561 aatcgtcggg tgctttttac caagttatta gcaggtaaga aaaattcatc aacggaaaca
    16621 tgaagtaacg atacaaggtc ataaagaact tgtatgctgg ggtgttgccc tttattttca
    16681 atattagtta agtaccgtgg gtcaatttca atcaatgctc ccacttgttc acgagttaaa
    16741 cctcgtttca atcgagcttc tttaatggct aaaccaaagg ctctaaaatc atatttatct
    16801 tcttttttac gcatagtaga ccacctctat acattttatt gttcctactg aattaaaaac
    16861 agg
//
LOCUS       DATRNX010000217         2670 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699723-rid18552253.guided.157, whole genome shotgun sequence.
ACCESSION   DATRNX010000217 DATRNX010000000
VERSION     DATRNX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699723
            Sequence Read Archive: SRR7789209
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790077.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790077.1
            Genome Coverage       :: 125x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:19:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,063
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,962
            CDSs (with protein)               :: 1,962
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 40 of 60
            Pseudo Genes (incomplete)         :: 7 of 60
            Pseudo Genes (internal stop)      :: 22 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699723-rid18552253.guided.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-05-14"
                     /collected_by="RMPRU"
     gene            <1..173
                     /locus_tag="VBC81_002060"
     CDS             <1..173
                     /locus_tag="VBC81_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6913032.1"
                     /translation="MKKPHACVIKETGKKANQWKVLRVGADIKIQCTNCQHVIMMSRY
                     DFERKLKKVLQP"
     gene            779..1252
                     /gene="dfrF"
                     /locus_tag="VBC81_002061"
     CDS             779..1252
                     /gene="dfrF"
                     /locus_tag="VBC81_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEO6913033.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1359..1889
                     /locus_tag="VBC81_002062"
     CDS             1359..1889
                     /locus_tag="VBC81_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6913034.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            2435..>2670
                     /locus_tag="VBC81_002063"
     CDS             2435..>2670
                     /locus_tag="VBC81_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HEO6913035.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITSHKV"
BASE COUNT          962 a          343 c          556 g          809 t
ORIGIN      
        1 aaatgaaaaa acctcatgct tgtgtaatta aagaaactgg taaaaaggct aatcaatgga
       61 aggtactgag agtaggagct gatattaaaa ttcagtgtac taactgtcag catgtgatta
      121 tgatgagtcg ttatgatttt gagcgaaaat taaaaaaagt actgcaacca tgaaagtcct
      181 tttaaacaaa taattatagc tatatttgca accaatactt tcctaaataa ttgctagtat
      241 gccgttggaa aattagctat tctaatgtta tcgaaagaag aaaggtggcc attgacaatg
      301 acgaaagttg cagaacagtt gtttattttt cggtaagtca tatcaaaatg ggaaaatggt
      361 gtttcccaaa caaaaagata ttaagaaaca gcccatgttt acctgcttgc aaacaaaaga
      421 atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg
      481 tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag tctatggttt
      541 gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata
      601 agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag
      661 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
      721 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
      781 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
      841 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
      901 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
      961 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
     1021 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
     1081 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
     1141 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
     1201 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
     1261 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
     1321 taagacaaat tcctgtttgt atttataaaa gagaaaaaat gagaaaagta gttttattta
     1381 ttgccatgag ccttgacgga tatattgcgg acggtaatgg tggagtagct tggctaaatg
     1441 gtcatggaaa tgacaatgaa aacatcgaca cttatacaga atttaccaaa gatatagata
     1501 ctgtcttaat ggggtggaat acttctcatc aggttgttac tgaactttct ccgcaagaat
     1561 gggtatataa taaatttaca acttatgtat taacacataa agagtgcaat tcttcccaac
     1621 aaattcattt tacaagtgaa aatcctgttt tattattgga acggctaaaa caagaagcag
     1681 gaaaggatat ttggatttgt ggcggagcaa atcttgttca gcagttggta agcaaaaata
     1741 tgattgataa atattacatt tctgttattc ctactctgtt aggaaatggg gttcgtcttt
     1801 tgggcaacat agacagggaa ataaagttaa ggctttgtaa aacacagact tataacggaa
     1861 taaccgattt gatttatatg cgtagataac tagagagaaa cagaaaagct gttaaaccga
     1921 cggagcgatc cgaccacgtt ggcgggaaaa tccttaagat ttttccgcca tgcttgcaag
     1981 ggggcggttc gcttcggcgg ttcggcgagc ttgcgagccg ttcaaagccc ccgttatcta
     2041 acaagggggg cacaaaaaac aagaaacact atacactaac aagcggaaaa cgctgtattt
     2101 ataaggcaaa accccatttt tacagtagtg atataaattg tacctatatc actatgaaaa
     2161 ttgaatagta aatatgagaa agacagttga tttcaaaaaa taagaaattg attgtcgttt
     2221 tcttttgcag aaatttaagt gcaggcaatt atggtagttg tcgtaattgc tgtcagctca
     2281 gtgtttatca ctgatggtgt gaaagattta cggttggaga tgttcaataa gattattggt
     2341 gcttaattat ttttattgta gtgctgatat gagaatgtta taattggggt ataaaacata
     2401 caaataaatt tgctgaaaaa tgaggtatca aaatatggaa aatgaattac tggaaaactt
     2461 aggaagactt cacacaactg aacttggcgt tattagaatt aaaaagaact tatctttgaa
     2521 tgtagaaaat gtaattgaat ggtgcaaaga aaaaattagc ctttctaatg cggaaatcac
     2581 aagaaaagga aaaaactggt atataacgat tgataattgt attataacgg taaatgcgta
     2641 cagttatacc gttatcactt ctcacaaggt
//
